研究者基本情報

• 博士（獣医学）, Zagazig大学

• https://researchmap.jp/YaserElewa

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201701020463950360
• https://www.vetmed.hokudai.ac.jp/organization/anat/index.html
• https://l-station.cris.hokudai.ac.jp/research/2934/

• 30782221

• 201701020463950360

• immunotherapy
• Mucosa associated lymphoid tissue
• Bone disorder
• Reproductive disorders and infertility
• Kidney diseases
• Lymphocytes
• Mediatinal fat-associated Lymphoid clusters
• Innate immunity
• autoimmunity
• Respiratory diseases

• ライフサイエンス, 細胞生物学, Role of Mediastinal Fat associated lymphoid clusters in respiratory diseases Pathogenesis in human and animals

• 博士課程, 獣医学院

研究活動情報

• Integrative Metabolomic, Network Pharmacology, and Experimental Evidence for Lepidium sativum Seed Extract as a Natural Modulator of Pulmonary Fibrosis via the ncNRFR/Let-7d Regulatory Pathway
  Ibrahim M. Alanazi; Hebatallah H. Abo Nahas; Doaa I. Mohamed; Nora Hosny; Yaser H. A. Elewa; Manisha Agarwal; Ibrahim Abdel Aziz Ibrahim; Alaa Hisham Falemban; Ghazi A. Bamagous; Emad Rashad Sindi; Tarek A. Yousef; Sanchaita Rajkhowa; Maha Alsunbul; Essa M. Saied
  Pharmaceuticals, 2025年11月28日
  研究論文（学術雑誌）
• IMMUNOHISTOCHEMICAL AND HISTOMORPHOMETRIC RELATED SMALL INTESTINE CHANGES ASSOCIATED WITH SODIUM BUTYRATE SUPPLEMENT IN CHICKENS
  Mohamed A.M. Alsafy; Samar M. Ez Elarab; Islam A. Abdellatif; Yaser H. Elewa; Heba A. Basha; Naglaa F. Bassuoni; Samir A. El-Gendy; Mohamed A. Abumandour; Catrin Rutland; Karam Roshdy
  Slovenian Veterinary Research, 62, 3, 209, 22, University of Ljubljana, 2025年09月30日
  研究論文（学術雑誌）, This study evaluated the effects of sodium butyrate (SB) supplementation on small intestine development in broiler chickens. Periodic acid-Schiff (PAS) and immunohistochemistry were used to undertake histological examinations of the duodenum, jejunum, and ileum. Duodenum, jejunum, and ileum histomorphometric data (villus length, crypt depth, goblet cell count), and interleukin-22 (IL-22) and toll-like receptor 8 (TLR8) immuno-stained area tissue coverage were quantified in control and SB supplemented groups. The histological changes in the SB supplemented group compared to the control group were as follows: There were increased villi lengths, widths, and crypt depths in the small intestine (duodenum, jejunum, and ileum). Increased numbers of goblet cells were observed, especially in the ileum. In addition, the lymphoid tissue within the small intestine was significantly larger (cross-section area=SB 34.8±0.5m2 vs control 13.2±0.5m2) and presented with more lymphoid nodules and more diffuse lymphoid tissue in the tunica submucosa, in the SB supplemented group compared to controls. Chickens do not have lymph nodes, therefore the mucosal-associated lymphoid tissue plays a major immunological role. Significant immunohistochemistry expression of IL-22 and TLR8 proteins were observed in the intestinal epithelial layer of the small intestine, which may play a role in protecting against many pathogens and gastrointestinal cancers.Imunohistokemične in histomorfometrične spremembe tankega črevesa, povezane z dodatkom natrijevega butirata pri piščancihIzvleček: Raziskava je ocenila učinke dodajanja natrijevega butirata (SB) na razvoj tankega črevesa pri piščancih brojlerjih. Za histološke preiskave dvanajstnika, teščega in vitega črevesa sta bili uporabljeni periodična kislina-Schiff (PAS) in imunohistokemija. Histomorfometrični podatki za dvanajstnik, tešče in vito črevo (dolžina resic, globina kripte, število čašastih celic) ter imunohistokemična obarvanost tkiva z interlevkinom-22 (IL-22) in Tollu podobnim receptorjem 8 (TLR8) so bili izmerjeni v kontrolni skupini in skupini, ki je prejemala dodatek SB. Histološke spremembe v skupini z dodatkom SB so bile v primerjavi s kontrolno skupino naslednje: v tankem črevesu (dvanajstniku, teščem in vitem črevu) so se povečale dolžine, širine in globine kript. Povečano je bilo število čašastih celic, zlasti v vitem črevesu. Poleg tega je bilo limfatično tkivo v tankem črevesu v skupini z dodatkom SB v primerjavi s kontrolno bistveno večje (površina prečnega prereza = SB 34,8 ± 0,5 m2, pri kontrolni skupini pa 13,2 ± 0,5 m2) in je pokazalo več limfatičnih vozličev ter bolj razpršeno limfatično tkivo v podsluznici. Piščanci nimajo bezgavk, zato ima limfatično tkivo sluznice črevesa pomembno imunološko vlogo. V epiteliju tankega črevesa smo opazili značilno imunohistokemično izražanje proteinov IL-22 in TLR8, kar lahko ima vlogo pri zaščiti pred številnimi patogeni in rakom prebavil.Ključne besede: IL-22; TLR8; črevesne resice; natrijev butirat; čašaste celice; limfatični vozliči
• Copper Chelation by Penicillamine Protects Against Doxorubicin-Induced Cardiomyopathy by Suppressing FDX1-Mediated Cuproptosis
  Mohammad El-Nablaway; Hany M. A. Sonpol; Yaser Hosny Ali Elewa; Mohamed A. M. Ali; Mohamed Adel; Eman Serry Zayed; Maha Alhelf; Manar A. Didamoony; Amal Fahmy Dawood; Eman M. Embaby; Khaled S. El-Bayoumi; Wesam S. El-Saeed
  Biomolecules, 2025年09月15日
  研究論文（学術雑誌）
• Mesenchymal Stem Cells Mitigate Sterile Splenic Inflammation and Histoarchitectural Alterations in Hypothyroid Rats
  Arigue A Dessouky; Nesma I El-Naseery; Yaser H A Elewa; Eman I El-behery; Enas El-Hady
  Microscopy and Microanalysis, 31, 5, Oxford University Press (OUP), 2025年09月03日
  研究論文（学術雑誌）, Abstract
  
  Sterile inflammation (SI) is a common pathology in numerous diseases, as a cause or consequence. Hypothyroidism (HT) is an inflammatory condition with complications stemming from excess cytokine release. A thyroidectomy-induced HT rat model was used to evaluate the impact of human umbilical cord blood–derived mesenchymal stem cells (hUCB-MSCs) on splenic inflammation and histoarchitecture. Thirty male Wistar rats were allocated equally as Sham, HT and HT+hUCB-MSCs groups. HT was confirmed by serum-free thyroxin 3, thyroxin 4 and thyroid stimulating hormone levels. Splenic homogenates were analyzed for oxidative stress and inflammation markers. Histopathological and immunohistochemical analyses were conducted to assess splenic stromal and parenchymal elements, high mobility group box 1 (HMGB1), toll-like receptor 4 (TLR4), pro-inflammatory cytokine immune expression, in addition to the innate and adaptive immune cell populations and their proliferative and apoptotic activities. Correlations between HMGB1 level and various parameters were evaluated. hUCB-MSCs significantly downregulated HMGB1, TLR4 and pro-inflammatory cytokine immune expression and attenuated immune cell activation through increasing antioxidant status, attenuating lipid peroxidation, and modulating cellular proliferation and apoptosis, thus protecting the spleen against HT-induced structural damage and immune cell overactivation. Collectively, our results demonstrate the therapeutic efficacy of hUCB-MSCs in mitigating HT-induced SI.
• Harnessing the composition of lipid nanoparticles to selectively deliver mRNA to splenic immune cells for anticancer vaccination
  Mahmoud A. Younis; Yusuke Sato; Yaser H. A. Elewa; Hideyoshi Harashima
  Drug Delivery and Translational Research, Springer Science and Business Media LLC, 2025年03月07日
  研究論文（学術雑誌）
• Autophagy modulators in type 2 diabetes: A new perspective
  Ayah Talal Zaidalkilani; Hayder M. Al‐kuraishy; Esraa H. Fahad; Ali I. Al‐Gareeb; Yaser Hosny Ali Elewa; Mahmoud Hosny Zahran; Athanasios Alexiou; Marios Papadakis; Ammar AL‐Farga; Gaber El‐Saber Batiha
  Journal of Diabetes, 2024年12月
  研究論文（学術雑誌）
• Kynurenine pathway in type 2 diabetes: Role of metformin
  Zainah Al‐Qahtani; Hayder M. Al‐kuraishy; Naif H. Ali; Yaser Hosny Ali Elewa; Gaber El‐Saber Batiha
  Drug Development Research, 2024年08月
  研究論文（学術雑誌）
• Expression Patterns of Grainyhead-Like 2 and Ovo-Like 2 in Mouse Mammary Gland Development During Pregnancy, Lactation, and Weaning
  Elfeky, M.; Matsuoka, S.; Yamamoto, I.; Elewa, Y.H.A.; Nakamura, K.; Suzuki, H.; Kamikawa, A.; Okamatu-Ogura, Y.; Kimura, K.
  Microscopy and Microanalysis, 30, 3, 2024年07月04日
  研究論文（学術雑誌）
• Sleep disorders cause Parkinson's disease or the reverse is true: Good GABA good night.
  Hayder M Al-Kuraishy; Ali I Al-Gareeb; Ali K Albuhadily; Yaser Hosny Ali Elewa; Ammar Al-Farga; Faisal Aqlan; Mahmoud Hosny Zahran; Gaber El-Saber Batiha
  CNS neuroscience & therapeutics, 30, 3, e14521, 2024年03月, ［国際誌］
  英語, 研究論文（学術雑誌）, BACKGROUND: Parkinson's disease (PD) is a progressive neurodegenerative brain disease due to degeneration of dopaminergic neurons (DNs) presented with motor and non-motor symptoms. PD symptoms are developed in response to the disturbance of diverse neurotransmitters including γ-aminobutyric acid (GABA). GABA has a neuroprotective effect against PD neuropathology by protecting DNs in the substantia nigra pars compacta (SNpc). It has been shown that the degeneration of GABAergic neurons is linked with the degeneration of DNs and the progression of motor and non-motor PD symptoms. GABA neurotransmission is a necessary pathway for normal sleep patterns, thus deregulation of GABAergic neurotransmission in PD could be the potential cause of sleep disorders in PD. AIM: Sleep disorders affect GABA neurotransmission leading to memory and cognitive dysfunction in PD. For example, insomnia and short sleep duration are associated with a reduction of brain GABA levels. Moreover, PD-related disorders including rigidity and nocturia influence sleep patterns leading to fragmented sleep which may also affect PD neuropathology. However, the mechanistic role of GABA in PD neuropathology regarding motor and non-motor symptoms is not fully elucidated. Therefore, this narrative review aims to clarify the mechanistic role of GABA in PD neuropathology mainly in sleep disorders, and how good GABA improves PD. In addition, this review of published articles tries to elucidate how sleep disorders such as insomnia and REM sleep behavior disorder (RBD) affect PD neuropathology and severity. The present review has many limitations including the paucity of prospective studies and most findings are taken from observational and preclinical studies. GABA involvement in the pathogenesis of PD has been recently discussed by recent studies. Therefore, future prospective studies regarding the use of GABA agonists in the management of PD are suggested to observe their distinct effects on motor and non-motor symptoms. CONCLUSION: There is a bidirectional relationship between the pathogenesis of PD and sleep disorders which might be due to GABA deregulation.
• In vivo investigation of the anti-liver fibrosis impact of Balanites aegyptiaca/ chitosan nanoparticles
  Zein, N.; Yassin, F.; Ayoub, H.G.; Elewa, Y.H.A.; Mohamed, S.K.A.; Mahmoud, M.H.; Elfeky, M.; Batiha, G.E.-S.; Zahran, M.H.
  Biomedicine and Pharmacotherapy, 172, 2024年
  研究論文（学術雑誌）
• Diosgenin alleviates D-galactose-induced oxidative stress in rats’ brain and liver targeting aging and apoptotic marker genes
  El-Far, A.H.; Elghaity, M.M.; Mohamed, S.A.; Noreldin, A.E.; Elewa, Y.H.A.; Al Jaouni, S.K.; Alsenosy, A.A.
  Frontiers in Molecular Biosciences, 11, 2024年
  研究論文（学術雑誌）
• Morphological and Prognostic Values of Skin Lesions in Patients with COVID‐19
  Mahmoud A. Rageh; Ibrahim H. E. Yousef; Yaser Hosny Ali Elewa; Mofreh Mansour; Omar AbdelHady Omar Ahmed; Sameh Fawzy Fahmy; Ahmad Saeed Aladl; Mohamed Amer; Emad El Rewiny; Manar Elsayed Sallam; Amr Mohammad Ammar; Salma S. Mohammed; Ahmed Shawky
  Dermatology Research and Practice, 2024年01月
  研究論文（学術雑誌）
• Impact of intravenous/intranasal polyinosinic-polycytidylic acid administration on the mediastinal fat-associated lymphoid clusters and lung tissue in healthy mice.
  Yaser Hosny Ali Elewa; Alaa M Khalifa; Mahmoud Hosny Zahran
  Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 250, 152158, 152158, 2023年10月, ［国際誌］
  英語, 研究論文（学術雑誌）, BACKGROUND: Polyinosinic-polycytidylic acid (pIC) is a synthetic analog of double-stranded RNA. It is used as a synthetic adjuvant to induce an adaptive immune response. However, the effect of pIC on the development of mediastinal fat-associated lymphoid clusters (MFALCs) that regulate intrathoracic hemostasis has remained unidentified. METHODS: We investigated the impact of intranasal (i.n.) administration (pIC i.n. group) and intravenous (i.v.) administration (pIC i.v. group) of pIC on both MFALCs and lung tissue. RESULTS: Compared with the control phosphate-buffered saline (PBS) groups, both pIC-administered groups displayed a significant increase in the MFALC size (particularly in the pIC i.n. group), area of MFALC high endothelial venules (HEVs), area of lymphatic vessels (LVs), number of proliferating cells (particularly in the pIC i.v. group), and number of immune cells (B220+ B-lymphocytes, CD3+ T-lymphocytes, Iba1+ macrophages, and Gr-1+ granulocytes) in both MFALCs and lung tissues. In addition, a positive correlation was detected between MFALC size and proliferating cells, immune cell population, LVs, and HEVs within MFALCs in both groups. Except for the proliferating cell and B-lymphocyte populations in the i.n. administered group and granulocyte populations in both i.n. and i.v. administered routes, such correlations were significant. CONCLUSION: In all, our data indicate that local or systemic administration of pIC induces the development of MFALCs and can be used as an immunostimulant therapeutic strategy.
• Calbindin Has a Potential Spatiotemporal Correlation with Proliferation and Apoptosis in the Postnatal Rat Kidney
  Mahmoud, S.F.; Elewa, Y.H.; Nomir, A.G.; Rashwan, A.M.; Noreldin, A.E.
  Microscopy and Microanalysis, 29, 5, 2023年09月29日
  研究論文（学術雑誌）
• Reprogramming activated hepatic stellate cells by siRNA-loaded nanocarriers reverses liver fibrosis in mice
  Mahmoud A. Younis; Yusuke Sato; Yaser H.A. Elewa; Hideyoshi Harashima
  Journal of Controlled Release, 361, 592, 603, 2023年09月
  研究論文（学術雑誌）
• Harnessing Topology and Stereochemistry of Glycidylamine‐Derived Lipid Nanoparticles for in Vivo mRNA Delivery to Immune Cells in Spleen and Their Application for Cancer Vaccination
  Mahmoud M. Abd Elwakil; Ryota Suzuki; Alaa M. Khalifa; Rania M. Elshami; Takuya Isono; Yaser H.A. Elewa; Yusuke Sato; Takashi Nakamura; Toshifumi Satoh; Hideyoshi Harashima
  Advanced Functional Materials, Wiley, 2023年07月26日
  研究論文（学術雑誌）, Abstract
  
  mRNA lipid nanoparticles (LNPs) have reached an inflection point and are now paving the way for a new wave of precision therapies. The design of nonhepatocyte RNA delivery systems without targeting ligands, however, remains a challenge. It is reported that the development of ligand‐free glycidylamine (GA) derivatives containing LNPs (GA‐LNPs) that preferentially deliver mRNA to immune cells in the spleen. Notably, it is demonstrated that the stereochemistry of GA‐lipids has a significant impact on their self‐assembly and in vitro and in vivo RNA delivery efficiency and tropism. This impact is dependent on the monomeric structure of GA and number of stereogenic centers. Furthermore, the nonlinear topology of GA lipid derivatives induced a sevenfold improvement in mRNA delivery efficiency. The top‐performing estriol‐GA05‐30 LNPs elicited strong antitumor activity in a therapeutic and prophylactic cancer model and are well tolerated in mice. These results highlight the significance of the chemistry of ionizable lipids for extrahepatic RNA delivery and indicated a promising direction for the development of next‐generation mRNA immunotherapies.
• Apitoxin alleviates methyl mercury-induced peripheral neurotoxicity in male rats by regulating dorsal root ganglia neuronal degeneration and oxidative stress
  Moustafa S. Abdelhamid; Khlood M. El.Bohi; Mohamed H. Sherif; Manar S. Abdelhamid; Mohamed M. Abdel-Daim; Yaser H.A. Elewa; Mohamed M.M. Metwally; Ghadeer M. Albadrani; Agnieszka Najda; Shereen El. Abdel-Hamid; Ehsan H. Abu-Zeid
  Biomedicine & Pharmacotherapy, 161, 114521, 114521, Elsevier BV, 2023年05月
  研究論文（学術雑誌）
• Parkinson’s Disease Risk and Hyperhomocysteinemia: The Possible Link
  Hayder M. Al-kuraishy; Ali I. Al-Gareeb; Yaser Hosny Ali Elewa; Mahmoud Hosny Zahran; Athanasios Alexiou; Marios Papadakis; Gaber El-Saber Batiha
  Cellular and Molecular Neurobiology, 43, 6, 2743, 2759, Springer Science and Business Media LLC, 2023年04月19日, ［国際誌］
  英語, 研究論文（学術雑誌）, Abstract
  
  Parkinson’s disease (PD) is one of the most common degenerative brain disorders caused by the loss of dopaminergic neurons in the substantia nigra (SN). Lewy bodies and -synuclein accumulation in the SN are hallmarks of the neuropathology of PD. Due to lifestyle changes and prolonged L-dopa administration, patients with PD frequently have vitamin deficiencies, especially folate, vitamin B6, and vitamin B12. These disorders augment circulating levels of Homocysteine with the development of hyperhomocysteinemia, which may contribute to the pathogenesis of PD. Therefore, this review aimed to ascertain if hyperhomocysteinemia may play a part in oxidative and inflammatory signaling pathways that contribute to PD development. Hyperhomocysteinemia is implicated in the pathogenesis of neurodegenerative disorders, including PD. Hyperhomocysteinemia triggers the development and progression of PD by different mechanisms, including oxidative stress, mitochondrial dysfunction, apoptosis, and endothelial dysfunction. Particularly, the progression of PD is linked with high inflammatory changes and systemic inflammatory disorders. Hyperhomocysteinemia induces immune activation and oxidative stress. In turn, activated immune response promotes the development and progression of hyperhomocysteinemia. Therefore, hyperhomocysteinemia-induced immunoinflammatory disorders and abnormal immune response may aggravate abnormal immunoinflammatory in PD, leading to more progression of PD severity. Also, inflammatory signaling pathways like nuclear factor kappa B (NF-κB) and nod-like receptor pyrin 3 (NLRP3) inflammasome and other signaling pathways are intricate in the pathogenesis of PD. In conclusion, hyperhomocysteinemia is involved in the development and progression of PD neuropathology either directly via induction degeneration of dopaminergic neurons or indirectly via activation of inflammatory signaling pathways.
• Microenvironmental Changes in Mediastinal Fat-associated Lymphoid Clusters and Lungs in Early and Late Stages of Metastatic Lung Cancer Induction
  Yaser Hosny Ali Elewa; Mahmoud M Abd Elwakil; Hideyoshi Harashima; Sherif Kh A Mohamed; Mahmoud Hosny Zahran
  Microscopy and Microanalysis, 29, 3, 1228, 1243, Oxford University Press (OUP), 2023年04月01日, ［国際誌］
  英語, 研究論文（学術雑誌）, Abstract
  
  The prognosis of metastatic lung melanoma (MLM) has been reported to be poor. An increasing number of studies have reported the function of several immune cells in cancer regression. Although the function of mediastinal fat-associated lymphoid clusters (MFALCs) in the progression of inflammatory lung lesions has been previously reported, the association between MLM progression and MFALCs development has remained unexplored. Herein, we compared the microenvironmental changes in the lungs and MFALCs among phosphate-buffered saline (PBS) and cancer groups at early (1 week) and late (2 weeks) stages following the intravenous injection of B16-F10 melanoma cells into C57BL/6 mice. Except for lung CD4+ helper T-cells and Iba1+ macrophage populations of early stage, we observed a significant increase in the proliferating and immune cell (CD20+ B-lymphocytes, CD3+ T-lymphocytes, CD8+ cytotoxic T-cells, CD16+ natural killer (NK) cells populations, area of high endothelial venules, and lung lymphatic vessels in cancer groups at both the stages as compared with the PBS groups. Furthermore, a significant positive correlation was observed between immune cell populations in MFALCs and the lungs (B- and T-lymphocytes, and NK cells in both stages). Collectively, our findings suggest a promising cancer therapeutic strategy via targeting immune cells in MFALCs.
• CHICKEN AIR SACS AND MESENTERY: A HISTOMORPHOMETRICAL AND IMMUNOLOGICAL STUDY
  Yaser Hosny Ali Elewa; Md Rashedul Islam; Sherif Kh. A. Mohamed
  SLOVENIAN VETERINARY RESEARCH, 60, 25-Suppl, University of Ljubljana, 2023年02月26日
  研究論文（学術雑誌）, Fat-associated lymphoid clusters (FALCs) are novel lymphoid tissues that have been reported in the mesenteric and mediastinal fat tissue of mouse and human. It plays role in the progression of respiratory and intestinal inflammation and parasitic infestations. However, their occurrence in the chicken air sacs and mesenteric adipose tissue has not yet been identified. Here we investigated the occurrence and distribution of FALCs in the air sacs (cervical, clavicular, thoracic, and abdominal) and mesenteric adipose tissue of healthy chicken. The latter air sacs and mesentery were immediately harvested after anesthesia and cutting the chicken heads then fixed in 4% paraformaldehyde fixative solution for histopathological examination. The degree of FALCs development among different specimens was measured and statistically analyzed. Our results revealed lymphoid clusters associating with the adipose tissues in mesentery, and air sacs (clavicular, thoracic, and abdominal), but not the cervical one. Interestingly, the thoracic air sacs showed significant higher FALCs size in comparison to that of other air sac types and the mesentery. Our findings suggested other possible immunological role of the air sacs and mesentery that could have impact on the progression of air sacculitis and mesenteritis- associate diseases. However, further investigations are required for clarification of air sacs and mesenteric FALCs in the progressions of respiratory and digestive tract diseases.
• Human umbilical cord blood-derived mesenchymal stem cells restored hematopoiesis by improving radiation induced bone marrow niche remodeling in rats
  El-Naseery, N.I.; Elewa, Y.H.A.; El-behery, E.I.; Dessouky, A.A.
  Annals of Anatomy, 250, 2023年
  研究論文（学術雑誌）
• Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches
  Shaimaa M. Saafan; Shymaa A. Mohamed; Ahmed E. Noreldin; Fayza A. El Tedawy; Yaser H. A. Elewa; Reda S. Fadly; Soad Khalil Al Jaouni; Ali H. El-Far; Abdelwahab A. Alsenosy
  Food & Function, 14, 12, 5728, 5751, Royal Society of Chemistry (RSC), 2023年
  研究論文（学術雑誌）, Oxidative stress results from the imbalance between reactive oxygen species (ROS) production and antioxidant defence and is primarily involved in aging.
• Self-homing nanocarriers for mRNA delivery to the activated hepatic stellate cells in liver fibrosis
  Mahmoud A. Younis; Yusuke Sato; Yaser H.A. Elewa; Yasuhiro Kon; Hideyoshi Harashima
  Journal of Controlled Release, 353, 685, 698, Elsevier BV, 2023年01月
  研究論文（学術雑誌）
• Barhi date (Phoenix dactylifera) extract ameliorates hepatocellular carcinoma in male rats.
  Nabila Zein; Yaser Hosny Ali Elewa; Mohammed K Alruwaili; Michel Dewaard; Mohammed Alorabi; Sarah M Albogami; Gaber El-Saber Batiha; Mahmoud Hosny Zahran
  Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 156, 113976, 113976, 2022年12月, ［国際誌］
  英語, 研究論文（学術雑誌）, Hepatocellular carcinoma (HCC) is a malignant tumor with limited treatment options. Given this fact, it may be important to develop new molecular targeted therapies from natural products, especially those which are primary sources of effective anticancer drugs with distinct mechanisms. Moreover, the complementary use of traditional herbs or fruit may increase the possibility of finding curative options for cancer. Here we explore the anticancer effects and possible molecular mechanism of Barhi date extract using an HCC rat model. Thirty two male albino rats were arbitrarily allocated into four groups: a negative control group (NCG); a positive control group (PCG), which received CCl4 (1 ml/kg b.wt./ i.p.) twice a week for three months; a Barhi date extract (400 mg/kg b.wt./day/orally) treatment group (DTG) during the third month of CCl4 administration; and a cisplatin (1.5 mg/kg b.wt./ i.p.) treatment group ( CTG) during the third month of CCl4 administration. After treatment we performed biochemical analyses of all groups to assess relative eukaryotic initiation factor 2 alpha (eIF2α), extracellular signal-regulated kinases (ERKs), protein kinase RNA-like endoplasmic reticulum kinase (PERK), poly (ADP-ribose) polymerase (PARP), and CASPASE 3 protein content, and examined expression of the genes phosphatase and tensin homolog (PTEN) and protein kinase B (AKT). We also performed an immunohistochemistry assay for alpha-fetoprotein (AFP). Our data showed higher PARP and CASPASE3 levels and liver enzymes (alanine aminotransferase [ALT], aspartate aminotransferase [AST], and alkaline phosphatase [ALP]) in the PCG compared to the DTG and the cisplatin treatment group CTG. However, we also found a significant decrease in PTEN in the PCG relative to both the DTG and the CTG. We conclude that the anti-tumor activity of Barhi date extract may be mediated by the inhibition of cell proliferation and apoptosis via the ERK /PARP/caspase3 pathway and the AKT/ PTEN signaling pathways.
• Cellular infiltration, cytokines, and histopathology of skin lesions associated with different clinical forms and stages of naturally occurring lumpy skin disease in cattle
  Yassien Badr; Ahmed E. Noreldin; Yaser Hosny Ali Elewa; Mohamed S. Ahmed; Yasuo Inoshima; Nabil M. Baker; Waleed Nabih Aamer; Osama M. Abas; Mohamed Nayel; Md. Matiur Rahman; Emad Elgendy; Asmaa G. Saleh; Mahmoud S. El-neweshy
  Comparative Immunology, Microbiology and Infectious Diseases, 90-91, 101894, 101894, Elsevier BV, 2022年11月, ［国際誌］
  英語, 研究論文（学術雑誌）, Lumpy skin disease (LSD) caused by the Capripoxvirus LSD virus which infects cattle, leading to a serious disease characterized by fever and the eruption of skin nodules all over the surface of the body. Our understanding of the pathogenesis of this disease is still incomplete, particularly the immunopathological alterations occurring in the skin nodules of infected animals. Therefore, we collected skin nodules from naturally infected cattle with different forms of the disease, both in the early stage of clinical infection and after disease progression. The skin samples were examined both histopathologically and immunohistochemically using a variety of antibodies targeting immune cellular markers and cytokines. As a result, the dermatohistopathology revealed orthokeratotic hyperkeratosis, vasculitis, epidermal microvesicles, and cellules claveleuses of Borrel in the early stage of infection, with the severity of the lesions correlating with the severity of the clinical disease. Meanwhile, late-stage samples had epidermal hyperkeratosis as well as dermal lymphocytic and histiocytic infiltrations. The predominant cellular infiltrates in the cutaneous lesions of early-stage LSD samples were interferon (IFN)-γ+ cells and CD4+ T lymphocytes with few macrophage lineage cells. However, in the late-stage samples, numerous Iba-1+ macrophages, with few IFN-γ+ cells and CD4+ T lymphocytes, were detected. Our findings indicate that IFN-γ+ cells, CD4+ T lymphocytes, and macrophages play a key role in the immunity against natural LSD virus infection and imply that cutaneous vasculopathy associated with LSD virus infection is an immune-mediated lesion. The current study contributes to our understanding of the pathogenesis of LSD.
• 全身性自己免疫異常がマウス骨格筋の外傷性損傷とその再生に与える影響について
  馬杉 実里; 市居 修; 大谷 祐紀; 難波 貴志; Elewa Yaser Hosny Ali; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 165回, [A2P, 07], (公社)日本獣医学会, 2022年09月
  日本語
• マウス急性腎障害期尿の尿路上皮バリアと免疫への作用
  市居 修; 難波 貴志; 大谷 祐紀; Elewa Yaser Hosny Ali; 中村 鉄平; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 165回, [A2P, 05], (公社)日本獣医学会, 2022年09月
  日本語
• BXSB/MpJ-Yaaマウスの病態からみる全身免疫と頭部粘膜免疫の関連
  平石 真也; 市居 修; 中村 鉄平; Elewa Yaser Hosny Ali; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 165回, [A2P, 13], (公社)日本獣医学会, 2022年09月
  日本語
• 妊娠期自己免疫疾患モデルマウスの胎盤と妊孕性の関連
  山中 里紗; 市居 修; 中村 鉄平; 大谷 祐紀; 難波 貴志; Hosny Ali Elewa Yaser; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 165回, [A3P, 06], (公社)日本獣医学会, 2022年09月
  日本語
• コットンラットの系統差比較が解き明かすヒト下咽頭梨状窩瘻の由来
  中村 鉄平; 細谷 実里奈; 市居 修; 渡邉 敬文; Elewa Yaser Hosny Ali; 入江 隆夫; 篠原 明男; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 165回, [A3P, 10], (公社)日本獣医学会, 2022年09月
  日本語
• Ureteral morphology and pathology during urolithiasis in cats.
  Osamu Ichii; Kazuhisa Oyamada; Hazuki Mizukawa; Nozomu Yokoyama; Takashi Namba; Yuki Otani; Yaser Hosny Ali Elewa; Noboru Sasaki; Teppei Nakamura; Yasuhiro Kon
  Research in veterinary science, 151, 10, 20, 2022年07月11日, ［国際誌］
  英語, 研究論文（学術雑誌）, Cats exhibit high susceptibility to urinary organ-related diseases. We investigated the healthy ureter morphologies and compared these with ureters that were surgically resected distal to a urolithiasis obstruction in cats. Healthy ureters (total length 9.88 ± 0.38 cm) developed adventitia composed of collagen fibers (ADCF), containing a longitudinal muscular layer, toward the distal segment. The healthy ureter was the smallest in the middle segment (4.71-6.90 cm from the urinary bladder) with significantly decreased luminal and submucosal areas compared to those in the proximal segment. Diseased cats exhibited a high incidence of calcium oxalate urolithiasis with renal dysfunction, regardless of age, sex, and body size. Diseased ureters showed increased perimeters, inflammation, and decreased nerves in ADCF. Collagen fibers were increased in the submucosal area, intermuscular spaces, and ADCF, particularly near the obstructed lesion. The mean resected ureter length was 5.66 ± 0.49 cm, suggesting a high obstruction risk in the middle segment. The middle segment also increased the cross-sectional area of the ureter and ADCF, regardless of the distance from the obstructed lesion. The ureters in several cases either lacked the transitional epithelium, or exhibited transitional epithelial hyperplasia, and some of these formed the mucosal folds. In conclusion, we demonstrated the following characteristics and histopathological features of cat ureters: decreases in the ureter size, lumen area, and submucosa area from proximal to middle segment in healthy; ADCF changes in urolithiasis, including increased connective tissues with inflammation and decreased nerves. These data are important to understand the pathogenesis of feline ureteral obstruction.
• Genital organ-associated lymphoid tissues arranged in a ring in the mucosa of cow vaginal vestibules.
  Tsolmon Chuluunbaatar; Osamu Ichii; Md Abdul Masum; Takashi Namba; Md Rashedul Islam; Yuki Otani; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Research in veterinary science, 145, 147, 158, 2022年07月, ［国際誌］
  英語, 研究論文（学術雑誌）, Female reproductive tracts are equipped with local and mucosal immune systems; however, structural information remains unclear for farm animals. In this study, the mucosa-associated lymphoid tissue-like structures in cow reproductive tracts were described. Vaginal vestibule (VV) and external parts of the genital organ, including the clitoris and vulva, were morphologically analyzed. Whole-mount specimens revealed several hematoxylin-positive spots arranged in a ring in the mucosa. Histologically, these spots were aggregated immune cells and defined as genital organ-associated lymphoid tissues (GOALTs). GOALTs were composed of lymphatic follicles (LFs) or diffuse lymphoid tissues (DLTs) at different depths of lamina propria. LFs frequently contained germinal centers. Scattered lymphocytes occupied the border area between follicles and epithelium, whereas DLTs had indefinite shapes. GOALTs contained immune cells and high endothelial venules. B cells were dominant both in LFs and DLTs. Abundant collagenous fibers were stretched across VV lamina propria, whereas reticular fibers were primarily observed in the DLT rather than LF. The epithelium covering of GOALTs was partially or fully disrupted by the invasion of immune cells toward the VV lumen. These findings suggest GOALTs function as a "genital lymphoid ring" as in Waldeyer's pharyngeal ring and act as immunological gate systems in cow reproductive tracts.
• Modified foreign body reaction to silicone imbedded in subcutaneous tissues by different mouse systemic immune conditions.
  Tomohiro Yamakawa; Osamu Ichii; Teppei Nakamura; Takashi Namba; Yaser Hosny Ali Elewa; Md Abdul Masum; Yuki Otani; Takanori Nishimura; Yasuhiro Kon
  Journal of biomedical materials research. Part A, 110, 12, 1921, 1931, 2022年06月30日, ［国際誌］
  英語, 研究論文（学術雑誌）, Foreign body reaction (FBR) causes unexpected adverse effects due to implanted materials in humans and animals. Inflammation and subsequent fibrosis during FBR seems to be affected by recipient immunity, such as the balance of T helper (Th) response that has the potential to regulate FBR-related macrophage function. Here, the immunological effects of FBR on subcutaneously imbedded silicone tubes (ST) at 8 weeks were investigated histologically by comparing Th1-biased C57BL/6N, Th2-biased MRL/MpJ, and autoimmune disease-prone MRL/MpJ-Faslpr/lpr . Tissue surrounding ST (TSS) was analyzed at day (D) 7 and 14 (reaction phase) or D35 (stability phase) after surgery. In all strains, the TSS was composed of a thin layer (TL) containing fibrous tissues and loose connective tissues formed outside the TL. Few lymphocytes and mast cells, several neutrophils, and numerous macrophages infiltrated the TSS. Active vascularization was observed at D14 in all strains. For the examined indices, M1-type macrophage density in the TSS of C57BL/6N mice was significantly higher at D14 compared to other strains. No significant strain difference relating to M2-type macrophages was detected, suggesting the effects of Th1-biased immunity on FBR-related inflammation. Collagen fibers in the TSS increased in density and became stable with age in all strains. In particular, MRL/MpJ-Faslpr/lpr showed progressive fibrotic features. Serum autoantibody levels in MRL/MpJ-Faslpr/lpr mice were inversely correlated with M1-type macrophage density. These data from MRL/MpJ-Faslpr/lpr mice suggested modifications of FBR-related inflammation and fibrosis by autoimmune abnormalities. The results provide crucial insights into the pathological modification of FBR by recipient immunity and emphasize its clinicopathological importance in humans and animals.
• Oral supplementation of policosanol alleviates carbon tetrachloride-induced liver fibrosis in rats
  Nabila Zein; Fathy Yassin; Shaza Makled; Saqer S. Alotaibi; Sarah M. Albogami; Gomaa Mostafa-Hedeab; Gaber El-Saber Batiha; Yaser Hosny Ali Elewa
  Biomedicine & Pharmacotherapy, 150, 113020, 113020, Elsevier BV, 2022年06月, ［国際誌］
  英語, 研究論文（学術雑誌）, Liver fibrosis is a prevalent liver disease that requires rapid and effective treatment prior to its progression to cirrhosis and liver damage. Recently, several reports have investigated the efficacy of phytotherapy using natural herbal extracts rather than synthetic drugs to treat several liver diseases. Policosanol is a herbal extract used to treat patients with cardiovascular. However, its therapeutic effect on liver fibrosis is still unknown. Therefore, the present study aimed to assess the potential antifibrotic effect of policosanol compared to silymarin and the possible underlying molecular mechanisms. Rats were categorized into four groups; negative control group "NCG", the fibrotic group "FG", silymarin treated group "STG", and policosanol treated group "PTG". Serum liver enzymes, oxidative stress markers, angiogenic growth factors, and pro-inflammatory cytokines were measured biochemically. The relative mRNA expressions of liver caspase-3 and alpha-smooth muscle actin (α-SMA) were assessed. Immunohistochemical staining was carried out using anti- α-SMA, and anti-caspase-3 antibodies. Compared to NCG, the FG group demonstrated a significant decrease in the level of serum liver enzymes "GSH, TAC, and SDF. Nevertheless, it demonstrateda significant increase in the level of pro-inflammatory cytokines "Il-6, TNF"; oxidative stress markers "NO, MDA", and angiogenic growth factors "VEGF and PDGF" and the expression of α-SMA, and Caspase-3. Interestingly, the values of these measurements were restored to normal levels in the treated groups, particularly the PTG. In conclusion, our data revealed the beneficial effects of co-administration of policosanol or silymarin on the fibrotic liver rat model and thus could be a promising natural therapeutic drug.
• Histopathological Impact of Bleomycin on Lung Injury and Development of Mediastinal Fat-Associated Lymphoid Clusters in the Lymphoproliferative Mouse Model.
  Yaser Hosny Ali Elewa; Osamu Ichii; Sherif Kh A Mohamed; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 1, 15, 2022年05月23日, ［国際誌］
  英語, 研究論文（学術雑誌）, The purpose of this study is to elucidate the impact of bleomycin on the degree of lung injury and development of mediastinal fat-associated lymphoid clusters (MFALCs) in the lymphoproliferative mouse model (MRL/MpJ-Faslpr/lpr “Lpr”) and its control strain (MRL/MpJ “MpJ”). We analyzed immune cells, the degree of proliferation, lymphatic vessels (LVs), and high endothelial venules (HEVs) in lungs and MFALCs in Lpr and MpJ mice on the 7th and 21st days following intranasal instillation of either bleomycin (BLM group) or PBS (PBS group). The BLM group showed a significant increase in the size of MFALCs, lung injury score, and positive area ratios of LVs, HEVs, and immune cells (especially macrophages, B- and T-lymphocytes) on both days 7 and 21. Interestingly, the lungs in the BLM group on day 21 showed higher collagen deposition and cellular infiltration in MpJ and Lpr, respectively. Moreover, significant positive correlations were observed between the size of MFALCs and lung injury. In conclusion, BLM could exert lung fibrosis or lymphoproliferative infiltration in chronic stages in MpJ and Lpr, respectively, and this varied effect could be due to the variations in the degree of immune cell proliferation and the development of LVs and HEVs among the studied strains.
• The Ameliorative Effect of Dexamethasone on the Development of Autoimmune Lung Injury and Mediastinal Fat-Associated Lymphoid Clusters in an Autoimmune Disease Mouse Model.
  Yaser Hosny Ali Elewa; Md Abdul Masum; Sherif Kh A Mohamed; Md Rashedul Islam; Teppei Nakamura; Osamu Ichii; Yasuhiro Kon
  International journal of molecular sciences, 23, 8, 2022年04月18日, ［国際誌］
  英語, 研究論文（学術雑誌）, In our previous study, we revealed the ameliorative therapeutic effect of dexamethasone (Dex) for Lupus nephritis lesions in the MRL/MpJ-Fas lpr/lpr (Lpr) mouse model. The female Lpr mice developed a greater number of mediastinal fat-associated lymphoid clusters (MFALCs) and inflammatory lung lesions compared to the male mice. However, the effect of Dex, an immunosuppressive drug, on both lung lesions and the development of MFALCs in Lpr mice has not been identified yet. Therefore, in this study, we compared the development of lung lesions and MFALCs in female Lpr mice that received either saline (saline group "SG") or dexamethasone (dexamethasone group "DG") in drinking water as a daily dose along with weekly intraperitoneal injections for 10 weeks. Compared to the SG group, the DG group showed a significant reduction in the levels of serum anti-dsDNA antibodies, the size of MFALCs, the degree of lung injury, the area of high endothelial venules (HEVs), and the number of proliferating and immune cells in both MFALCs and the lungs. A significant positive correlation was observed between the size of MFALCs and the cellular aggregation in the lungs of Lpr mice. Therefore, this study confirmed the ameliorative effect of Dex on the development of lung injury and MFALCs via their regressive effect on both immune cells' proliferative activity and the development of HEVs. Furthermore, the reprogramming of MFALCs by targeting immune cells and HEVs may provide a therapeutic strategy for autoimmune-disease-associated lung injury.
• Ghrelin and Jacalin Localization in Developing Rat Kidney
  Rashwan, A.M.; Noreldin, A.E.; Mahmoud, S.F.; Elewa, Y.H.A.; Nomir, A.G.
  Journal of Morphological Sciences, 39, 2022年
  研究論文（学術雑誌）
• Close Association Between Altered Urine-urothelium Barrier and Tertiary Lymphoid Structure Formation in the Renal Pelvis During Nephritis.
  Osamu Ichii; Marina Hosotani; Md Abdul Masum; Taro Horino; Teppei Nakamura; Takashi Namba; Yuki Otani; Yaser Elewa; Yasuhiro Kon
  Journal of the American Society of Nephrology : JASN, 33, 1, 88, 107, 2021年10月22日, ［国際誌］
  英語, 研究論文（学術雑誌）, Background: Kidneys with chronic inflammation develop tertiary lymphoid structures (TLSs). Infectious pyelonephritis is characterized by renal pelvis (RP) inflammation. However, the pathological features of TLSs, including their formation and association with non-infectious nephritis, are unclear. Methods: RPs from humans and mice that were healthy or had non-infectious chronic nephritis, were analyzed for TLS development, and the mechanism of TLS formation investigated using urothelium or lymphoid structure cultures. Results: Regardless of infection, TLSs in the RP, termed urinary tract-associated lymphoid structures (UTALSs), formed in humans and mice with chronic nephritis. Moreover, urine played a unique role in UTALS formation. Specifically, we identified urinary IFN-γ as a candidate factor affecting urothelial barrier integrity because it alters occludin expression. In a nephritis mouse model, urine leaked from the lumen of the RP into the parenchyma. In addition, urine immunologically stimulated UTALS-forming cells via cytokine (IFN-γ, TNF-α) and chemokine (CXCL9, CXCL13) production. CXCL9 and CXCL13 were expressed in UTALS stromal cells and urine stimulation specifically induced CXCL13 in cultured fibroblasts. Characteristically, type XVII collagen (BP180), a candidate autoantigen of bullous pemphigoid, was ectopically localized in the urothelium covering UTALSs and associated with UTALS development by stimulating CXCL9 or IL-22 induction via the TNF-α/FOS/JUN pathway. Notably, UTALS development indices were positively correlated with chronic nephritis development. Conclusion: TLS formation in the RP is possible and altered urine-urothelium barrier-basedUTALS formation may represent a novel mechanism underlying the pathogenesis of chronic nephritis, regardless of urinary tract infection.
• Albendazole-loaded cubosomes interrupt the ERK1/2-HIF-1α-p300/CREB axis in mice intoxicated with diethylnitrosamine: A new paradigm in drug repurposing for the inhibition of hepatocellular carcinoma progression
  Sameh Saber; Mohamed Nasr; Ahmed S. Saad; Ahmed A.E. Mourad; Naglaa A. Gobba; Ahmed Shata; Abdel-Moneim Hafez; Ramy N. Elsergany; Heba I. Elagamy; Eman El-Ahwany; Noha A. Amin; Samuel Girgis; Yaser H.A. Elewa; Mohamed H. Mahmoud; Gaber El-Saber Batiha; Magdy Abou El-Rous; Islam Kamal; Mohamed M.Y. Kaddah; Ahmed E. Khodir
  Biomedicine & Pharmacotherapy, 142, 112029, 112029, Elsevier BV, 2021年10月
  研究論文（学術雑誌）
• ヤギ腎臓における血管系リンパ組織の分類(Clarification of vasculature-associated lymphoid tissue in goat kidney)
  Masum Md. Abdul; 市居 修; Elewa Yaser Hosny Ali; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 164回, [AO, 25], (公社)日本獣医学会, 2021年09月
  英語
• 牛の腟前庭にみられたリンパ陰部輪(Genital Lymphoid Ring found in the vaginal vestibule of cow)
  Chuluunbaatar Tsolmon; 市居 修; Masum Md. Abdul; Elewa Yaser Hosny Ali; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 164回, [AO, 38], (公社)日本獣医学会, 2021年09月
  英語
• Interleukin-36受容体を欠損した自己免疫性腎炎モデルマウスの腎表現型解析
  難波 貴志; 市居 修; 岡村 匡史; 中野 堅太; 中村 鉄平; 大谷 祐紀; Abdul Masum Md.; Hosny Ali Elewa Yaser; 昆 泰寛
  日本獣医学会学術集会講演要旨集, 164回, [AO, 27], (公社)日本獣医学会, 2021年09月
  日本語
• Morphological Features of the Testis among Autoimmune Mouse Model and Healthy Strains
  Zeinab Shouman; Hany E. Marei; Ahmed Abd-Elmaksoud; Mohamed Kassab; Takashi Namba; Md. Abdul Masum; Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  Microscopy and Microanalysis, 27, 5, 1209, 1217, Oxford University Press (OUP), 2021年08月05日
  研究論文（学術雑誌）, Abstract
  
  Autoimmune diseases play a critical role in the progression of infertility in both sexes and their severity has been reported to increase with age. However, few reports have discussed their effect on the morphological features of the testis. Therefore, we compared the morphological alterations in the testes of autoimmune model mice (MRL/MpJ-Fas^lpr) and the control strain (MRL/MpJ) with those of their background strain (C57BL/6N) at 3 and 6 months. Furthermore, we analyzed the changes in spermatocytes, Sertoli cells, immune cells, and Zonula occludens-1 junctional protein by immunohistochemical staining. The MRL/MpJ-Fas^lpr mice showed a significant increase in the serum Anti-double stranded DNA antibody level, relative spleen weight, and seminiferous luminal area when compared with other studied two strains. In contrast, a significant decrease in the relative testis weight, and numbers of both Sertoli, meiotic spermatocyte was observed in MRL/MpJ-Fas^lpr and MRL/MpJ mice compared with C57BL/6N mice especially at 6 months. Similarly, Zonula occludens-1 junctional protein positive cells showed a significant decrease in the same strains at 6 months. However, no immune cell infiltration could be observed among the studied three strains. Our findings suggest that the increase in autoimmune severity especially with age could lead to infertility through loss of spermatogenic and Sertoli cells, rather than the disturbance of the blood–testis barrier.
• Bee venom Apis mellifera lamarckii rescues blood brain barrier damage and neurobehavioral changes induced by methyl mercury via regulating tight junction proteins expression in rat cerebellum
  Ehsan H. Abu-Zeid; Bouthaina A. Khalifa; Yaser H.A. Elewa; Ahmed H. Arisha; Tamer A. Ismail; Basma M. Hendam; Shereen El Abdel-Hamid
  Food and Chemical Toxicology, 154, Elsevier Ltd, 2021年08月01日
  英語, 研究論文（学術雑誌）
• Altered Renal Pathology in an Autoimmune Disease Mouse Model After Induction of Diabetes Mellitus.
  Shiori Hiramatsu; Osamu Ichii; Takashi Namba; Yuki Otani; Teppei Nakamura; Md Abdul Masum; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27, 4, 897, 909, 2021年08月, ［国際誌］
  英語, 研究論文（学術雑誌）, Diabetes mellitus (DM) is a predisposing factor for renal disorder progression and is referred to as diabetic kidney disease (DKD). However, there are no reports of DKD with an underlying autoimmune disorder. In this study, we compared the pathophysiological changes caused by DM induction after streptozotocin (STZ) injection in comparison with that in a control group receiving citrate buffer (CB) in the autoimmune disease model mice “BXSB/MpJ-Yaa” (Yaa) and the wild-type strain BXSB/MpJ. Both strains showed hyperglycemia after 12 weeks of STZ injection. Interestingly, the Yaa group developed membranous and proliferative glomerulonephritis, which tended to be milder glomerular lesions in the STZ group than in the CB group, as indicated by a decreased mesangial area and ameliorated albuminuria. Statistically, the indices for hyperglycemia and autoimmune abnormalities were negatively and positively correlated with the histopathological parameters for mesangial matrix production and glomerular proliferative lesions, respectively. STZ treatment induced renal tubular anisonucleosis and dilations in both strains, and they were more severe in Yaa. Significantly decreased cellular infiltration was observed in the Yaa group compared to the CB group. Thus, in DKD related to autoimmune nephritis, hyperglycemia modifies its pathology by decreasing the mesangial area and interstitial inflammation and aggravating renal tubular injury.
• Mesenchymal stem cells enhance AQP1 expression in the sublingual salivary gland of ovariectomized menopausal rat model
  Nesma I. El-naseery; Yaser H.A. Elewa; Mona A.A. Arafa; W. S. Sabbah; Arigue A. Dessouky
  Annals of Anatomy, 236, Elsevier GmbH, 2021年07月01日
  英語, 研究論文（学術雑誌）
• Compartmentalization of interleukin 36 subfamily according to inducible and constitutive expression in the kidneys of a murine autoimmune nephritis model
  Takashi Namba; Osamu Ichii; Teppei Nakamura; Md Abdul Masum; Yuki Otani; Marina Hosotani; Yaser Hosny Ali Elewa; Yasuhiro Kon
  CELL AND TISSUE RESEARCH, 2021年07月, ［国際誌］
  英語, 研究論文（学術雑誌）
• Spatiotemporal histological changes observed in mouse subcutaneous tissues during the foreign body reaction to silicone
  Sao Oe; Md. Abdul Masum; Osamu Ichii; Takanori Nishimura; Teppei Nakamura; Takashi Namba; Yuki Otani; Yasuhide Nakayama; Yaser Hosny Ali Elewa; Yasuhiro Kon
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 109, 7, 1220, 1231, 2021年07月, ［国際誌］
  英語, 研究論文（学術雑誌）
• Possible Crosstalk of the Immune Cells within the Lung and Mediastinal Fat-Associated Lymphoid Clusters in the Acute Inflammatory Lung Asthma-Like Mouse Model.
  Yaser Hosny Ali Elewa; Mahmoud Mansour Abd Elwakil; Osamu Ichii; Teppei Nakamura; Sherif Kh A Mohamed; Yasuhiro Kon
  International journal of molecular sciences, 22, 13, 2021年06月26日, ［国際誌］
  英語, 研究論文（学術雑誌）, Recently, we clarified the function of mediastinal fat-associated lymphoid clusters (MFALCs) in the progression of several respiratory diseases. However, their role has not yet been identified in the lung asthmatic condition. Hence, we compared the immune cells in lung and MFALCs of C57BL/6N mice on days 3 and 7 following intranasal instillation of either papain (papain group "PG") or phosphate buffer saline (PBS) (vehicle group "VG"). The PG showed significantly prominent MFALCs, numerous goblet cells (GCs), and higher index ratios of different immune cells (macrophages, natural helper cells (NHC), B- and T-lymphocytes) within the MFALCs and lung than in the VG on both days 3 and 7. Interestingly, a tendency of decreased size of MFALCs and a significant reduction in the number of GCs and immune cells were observed within the MFALCs and lung in the PG on day 7 than on day 3. Furthermore, the quantitative parameters of these immune cells in MFALCs were significantly and positively correlated with the size of MFALCs and immune cells in the lung. This suggested that the possible crosstalk between immune cells within MFALCs and the lung could play a critical role in the progression and recovery of the acute inflammatory lung asthma.
• Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart
  Ali H. El-Far; Yaser H. A. Elewa; Elsayeda-Zeinab A. Abdelfattah; Abdel-Wahab A. Alsenosy; Mustafa S. Atta; Khalid M. Abou-Zeid; Soad K. Al Jaouni; Shaker A. Mousa; Ahmed E. Noreldin
  International Journal of Molecular Sciences, 22, 13, 6839, 6839, MDPI AG, 2021年06月25日
  研究論文（学術雑誌）, D-galactose (D-gal) administration causes oxidative disorder and is widely utilized in aging animal models. Therefore, we subcutaneously injected D-gal at 200 mg/kg BW dose to assess the potential preventive effect of thymoquinone (TQ) and curcumin (Cur) against the oxidative alterations induced by D-gal. Other than the control, vehicle, and D-gal groups, the TQ and Cur treated groups were orally supplemented at 20 mg/kg BW of each alone or combined. TQ and Cur effectively suppressed the oxidative alterations induced by D-gal in brain and heart tissues. The TQ and Cur combination significantly decreased the elevated necrosis in the brain and heart by D-gal. It significantly reduced brain caspase 3, calbindin, and calcium-binding adapter molecule 1 (IBA1), heart caspase 3, and BCL2. Expression of mRNA of the brain and heart TP53, p21, Bax, and CASP-3 were significantly downregulated in the TQ and Cur combination group along with upregulation of BCL2 in comparison with the D-gal group. Data suggested that the TQ and Cur combination is a promising approach in aging prevention.
• Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (Sigmodon hispidus).
  Md Rashedul Islam; Osamu Ichii; Teppei Nakamura; Takao Irie; Akio Shinohara; Md Abdul Masum; Yuki Otani; Takashi Namba; Tsolmon Chuluunbaatar; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Animals : an open access journal from MDPI, 11, 6, 2021年06月12日, ［国際誌］
  英語, 研究論文（学術雑誌）, Most mammalian ovarian follicles contain only a single oocyte having a single nucleus. However, two or more oocytes and nuclei are observed within one follicle and one oocyte, respectively, in several species, including cotton rat (CR, Sigmodon hispidus). The present study compared ovarian histology, focusing on folliculogenesis, between two inbred CR strains, HIS/Hiph and HIS/Mz. At 4 weeks of age, ovarian sections from both the strains were analyzed histologically. Multi-oocyte follicles (MOFs) and double-nucleated oocytes (DNOs) were observed in all stages of developing follicles in HIS/Hiph, whereas HIS/Mz had MOFs up to secondary stages and lacked DNOs. The estimated total follicles in HIS/Mz were almost half that of HIS/Hiph, but interstitial cells were well developed in HIS/Mz. Furthermore, immunostaining revealed no clear strain differences in the appearance of oocytes positive for Ki67, PCNA, and p63 in MOF or DNOs; no cell death was observed in these oocytes. Ultrastructural analysis revealed more abundant mitochondrial clouds in oocytes of HIS/Hiph than HIS/Mz. Thus, we clarified the strain differences in the CR ovary. These findings indicate that early events during folliculogenesis affect the unique ovarian phenotypes found in CRs, including MOFs or DNOs, and their strain differences.
• Ameliorative Role of Cerium Oxide Nanoparticles Against Fipronil Impact on Brain Function, Oxidative Stress, and Apoptotic Cascades in Albino Rats
  Norhan Elshony; Atef M. K. Nassar; Yasser S. El-Sayed; Dalia Samak; Ahmed Noreldin; Lamiaa Wasef; Hamida Saleh; Yaser H. A. Elewa; Shereen E. Tawfeek; Abdullah A. Saati; Gaber El-Saber Batiha; Michał Tomczyk; Masakazu Umezawa; Hazem M. Shaheen
  Frontiers in Neuroscience, 15, Frontiers Media SA, 2021年05月14日
  研究論文（学術雑誌）, Fipronil (FIP) is an N-phenylpyrazole insecticide that is used extensively in public health and agriculture against a wide range of pests. Exposure to FIP is linked to negative health outcomes in humans and animals including promoting neuronal cell injury, which results in apoptosis through the production of reactive oxygen species (ROS). Therefore, the purpose of the current study was to investigate the neuroprotective effects of cerium oxide nanoparticles (CeNPs) on neuronal dysfunction induced by FIP in albino rats. Male rats were randomly classified into four groups: control, FIP (5 mg/kg bwt), CeNPs (35 mg/kg bwt), and FIP + CeNPs (5 (FIP) + 35 (CeNPs) mg/kg bwt), which were treated orally once daily for 28 consecutive days. Brain antioxidant parameters, histopathology, and mRNA expression of genes related to brain function were evaluated. The results revealed oxidative damage to brain tissues in FIP-treated rats indicated by the elevated levels of malondialdehyde (MDA) and nitric oxide (NO) levels and reduced activities of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx). On the other hand, the FIP’s group that was treated with CeNPs showed decrease in MDA and NO levels and increase in SOD and GPx enzymes activity. Besides, FIP-treated rats showed decreased butyrylcholinesterase (BuChE) activity in comparison to the FIP + CeNPs group. Moreover, FIP caused up-regulation of the expression of neuron-specific enolase (NSE), caspase-3, and glial fibrillary acidic protein (GFAP) but down-regulation of B-cell lymphoma-2 (BCL-2) expression. But the FIP + CeNPs group significantly down-regulated the GFAP, NSE, and caspase-3 and up-regulated the gene expression of BCL-2. Additionally, the FIP-treated group of rats had clear degenerative lesions in brain tissue that was reversed to nearly normal cerebral architecture by the FIP + CeNPs treatment. Immunohistochemical examination of brain tissues of rats-treated with FIP showed abundant ionized calcium-binding adaptor molecule 1 (Iba-1) microglia and caspase-3 and apoptotic cells with nearly negative calbindin and synaptophysin reaction, which were countered by FIP + CeNPs treatment that revealed a critical decrease in caspase-3, Iba-1 reaction with a strong calbindin positive reaction in most of the Purkinje cells and strong synaptophysin reaction in the cerebrum and cerebellum tissues. Based on reported results herein, CeNPs treatment might counteract the neurotoxic effect of FIP pesticide via an antioxidant-mediated mechanism.
• Unique localization of disseminated pancreas in the oesophagus of catfish (clarias gariepinus) with reference to sexual dimorphism
  Mayada W. Karkit; Hoda F. Salem; Mohammad H. Bareedy; Yaser H. A. Elewa
  Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia, 50, 3, 594, 603, Blackwell Publishing Ltd, 2021年05月01日
  英語, 研究論文（学術雑誌）
• Aging-Related Functional and Structural Changes in Renal Tissues: Lesson from a Camel Model
  Asmaa F. Khafaga; Yaser H. A. Elewa; Mustafa S. Atta; Ahmed E. Noreldin
  Microscopy and Microanalysis, 27, 3, 566, 578, Oxford University Press (OUP), 2021年03月10日
  研究論文（学術雑誌）, Abstract
• Ultra-small lipid nanoparticles encapsulating sorafenib and midkine-siRNA selectively-eradicate sorafenib-resistant hepatocellular carcinoma in vivo
  Mahmoud A. Younis; Ikramy A. Khalil; Yaser H. A. Elewa; Yasuhiro Kon; Hideyoshi Harashima
  JOURNAL OF CONTROLLED RELEASE, 331, 335, 349, 2021年03月
  英語, 研究論文（学術雑誌）
• Unique histological features of the tail skin of cotton rat (Sigmodon hispidus) related to caudal autotomy.
  Marina Hosotani; Teppei Nakamura; Osamu Ichii; Takao Irie; Yuji Sunden; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Takashi Mishima; Yasuhiro Kon
  Biology open, 10, 2, 2021年02月19日, ［国際誌］
  英語, 研究論文（学術雑誌）, Caudal autotomy in rodents is an evolutionarily acquired phenomenon enabling escape from predators, by discarding the tail skin after traumatic injuries. The histological mechanisms underlying caudal autotomy seem to differ among species. Cotton rats (Sigmodon hispidus), which are important laboratory rodents for human infectious diseases, possess a fragile tail. In this study, we compared the tail histology of cotton rats with that of laboratory rats (Rattus norvegicus), which have no fragility on their tail, to elucidate the process of rodent caudal autotomy. First, the cotton rats developed a false autotomy characterized by loss of the tail sheath with the caudal vertebrae remaining without tail regeneration. Second, we found the fracture plane was continuous from the interscale of the tail epidermis to the dermis, which was lined with an alignment of E-cadherin+ cells. Third, we found an obvious cleavage plane between the dermis and subjacent tissues of the cotton-rat tail, where the subcutis was composed of looser, finer, and fragmented collagen fibers compared with those of the rat. Additionally, the cotton-rat tail was easily torn, with minimum bleeding. The median coccygeal artery of the cotton rat had a thick smooth muscle layer, and its lumen was filled with the peeled intima with fibrin coagulation, which might be associated with reduced bleeding following caudal autotomy. Taken together, we reveal the unique histological features of the tail relating to the caudal autotomy process in the cotton rat, and provide novel insights to help clarify the rodent caudal autotomy mechanism.
• Novel lipid combination for delivery of plasmid DNA to immune cells in the spleen
  Seigo Kimura; Ikramy A. Khalil; Yaser H.A. Elewa; Hideyoshi Harashima
  Journal of Controlled Release, 330, 753, 764, Elsevier BV, 2021年02月
  研究論文（学術雑誌）
• Pathological Alternations of Mediastinal Fat-Associated Lymphoid Cluster and Lung in a Streptozotocin-Induced Diabetic Mouse Model.
  Yaser H A Elewa; Osamu Ichii; Teppei Nakamura; Yasuhiro Kon
  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27, 1, 187, 200, 2021年02月, ［国際誌］
  英語, 研究論文（学術雑誌）, Diabetes is a devastating global health problem and is considered a predisposing factor for lung injury progression. Furthermore, previous reports of the authors revealed the role of mediastinal fat-associated lymphoid clusters (MFALCs) in advancing respiratory diseases. However, no reports concerning the role of MFALCs on the development of lung injury in diabetes have been published. Therefore, this study aimed to examine the correlations between diabetes and the development of MFALCs and the progression of lung injury in a streptozotocin-induced diabetic mouse model. Furthermore, immunohistochemical analysis for immune cells (CD3+ T-lymphocytes, B220+ B-lymphocytes, Iba1+ macrophages, and Gr1+ granulocytes), vessels markers (CD31+ endothelial cells and LYVE-1+ lymphatic vessels "LVs"), and inflammatory markers (TNF-α and IL-5) was performed. In comparison to the control group, the diabetic group showed lung injury development with a significant increase in MFALC size, immune cells, LVs, and inflammatory marker, and a considerable decrease of CD31+ endothelial cells in both lung and MFALCs was observed. Furthermore, the blood glucose level showed significant positive correlations with MFALCs size, lung injury, immune cells, inflammatory markers, and LYVE-1+ LVs in lungs and MFALCs. Thus, we suggest that the development of MFALCs and LVs could contribute to lung injury progression in diabetic conditions.
• Osteoblast-activating peptide exhibits a specific distribution pattern in mouse ovary and may regulate ovarian steroids and local calcium levels
  Noreldin, A.E.; Gewaily, M.S.; Saadeldin, I.M.; Abomughaid, M.M.; Khafaga, A.F.; Elewa, Y.H.
  American Journal of Translational Research, 13, 6, 2021年
  研究論文（学術雑誌）
• Engineered ε-decalactone lipomers bypass the liver to selectively in vivo deliver mRNA to the lungs without targeting ligands
  Mahmoud M. Abd Elwakil; Tianle Gao; Takuya Isono; Yusuke Sato; Yaser H. A. Elewa; Toshifumi Satoh; Hideyoshi Harashima
  Materials Horizons, Royal Society of Chemistry (RSC), 2021年
  研究論文（学術雑誌）,

  Engineered lipomers coming from sustainable sources can efficiently bypass the liver to deliver a genetic message to the lungs after systemic administration without targeting ligands.

• Anatomy and histology of the foramen of ovarian bursa opening to the peritoneal cavity and its changes in autoimmune disease-prone mice.
  Marina Hosotani; Osamu Ichii; Teppei Nakamura; Takashi Namba; Md Rashedul Islam; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Yasuhiro Kon
  Journal of anatomy, 238, 1, 73, 85, 2021年01月, ［国際誌］
  英語, 研究論文（学術雑誌）, The ovarian bursa is a small peritoneal cavity enclosed by the mesovarium and mesosalpinx, which surrounds the ovaries and oviductal infundibulum in mammals. The ovarian bursa is considered as the structure facilitating the transport of ovulated oocytes into the oviduct. Our previous study revealed reduced oocyte pick-up function in the oviduct of lupus-prone MRL/MpJ-Faslpr / lpr mouse, suggesting the possibility of an escape of ovulated oocytes into the peritoneal cavity, despite the presence of an almost complete ovarian bursa in the mouse. In this study, we revealed anatomical and histological characteristics of the ovarian bursa in C57BL/6 N, MRL/MpJ, and MRL/MpJ-Faslpr / lpr mice. All strains had the foramen of ovarian bursa (FOB), with a size of approximately 0.04 to 0.12 cm2 , surrounded by the ligament of ovarian bursa (LOB), which is part of the mesosalpinx. The LOB was partially lined with the cuboidal mesothelial cells and consisted of a thick smooth muscle layer in all strains. In 6-month-old MRL/MpJ-Faslpr / lpr mice, in which the systemic autoimmune abnormality deteriorated and oocyte pick-up function was impaired, the size of the FOB tended to be larger than that of other strains. Additionally, in MRL/MpJ-Faslpr / lpr mice at 6 months of age, there was infiltration by numerous immune cells in the mesosalpinx suspending the isthmus; however, the LOB prevented severe inflammation and showed deposition of collagen fibers. These results not only indicate that the FOB is a common structure within mice, but also imply the physiological function of the LOB and its role in maintaining the microenvironment around the ovary, as well as regulating healthy reproduction.
• Dual Effect of Bleomycin on Histopathological Features of Lungs and Mediastinal Fat-Associated Lymphoid Clusters in an Autoimmune Disease Mouse Model.
  Yaser Hosny Ali Elewa; Osamu Ichii; Teppei Nakamura; Yasuhiro Kon
  Frontiers in immunology, 12, 665100, 665100, 2021年, ［国際誌］
  英語, 研究論文（学術雑誌）, Mediastinal fat-associated lymphoid clusters (MFALCs) are novel immune clusters that function in the pathogenesis of bleomycin (BLM)-induced pneumonitis in a C57BL/6 mouse model. However, we lack literature on the effects of BLM in an autoimmune disease mouse model (AIDM). In the present study, BLM sulfate (BLM group) or phosphate-buffered saline (PBS group) were intranasally administered in BXSB/MpJ-Yaa (Yaa) AIDM and its wild-type strains (BXSB/MpJ "BXSB") and the histopathology of MFALCs and lungs were examined on days 7 and 21 days. Immunohistochemical analysis was performed to detect lymphatic vessels (LVs), high endothelial venules (HEVs), proliferating, and immune cells. Furthermore, the mRNA expression of Yaa locus genes (TLR7, TLR8, Arhgap6, Msl3, and Tceanc) was detected in the lung tissues. Here, we show a dual effect of BLM on intra-thoracic immune hemostasis among Yaa AIDM and its corresponding wild-type strain (BXSB mice). The BLM group of BXSB mice displayed significantly higher values of lung injury scores (LIS) and size of MFALCs as compared with the corresponding PBS group. However, an opposite effect was detected in Yaa mice. Furthermore, Yaa mice displayed decreased serum autoantibody titers and downregulated expression of TLR7, TLR8, Msl3, and Tceanc in the lungs following BLM administration, especially on day 21. Interestingly, significant positive correlations were detected in both strains between the LIS and the size of MFALCs, LVs, HEVs, and proliferating cells. Conclusively, our findings revealed a crucial function of HEVs on the extent of lung injury and the development of MFALCs in BLM-administered Yaa AIDM and control BXSB mice with dual effects. Moreover, our data suggest that down regulation of Yaa locus genes could contribute as an important attributing factor leading to decrease in the degree of autoimmunity and lung injury in AIDM. Therefore, we suggest that genetic background contributes to BLM diversity among AIDM and the wild-type strain. Targeting some genes or venules could provide novel therapeutic approaches for some autoimmune-associated respiratory diseases via controlling the MFALCs development.
• The potential ameliorative impacts of cerium oxide nanoparticles against fipronil-induced hepatic steatosis
  Lamiaa Wasef; Atef M. K. Nassar; Yasser S. El-Sayed; Dalia Samak; Ahmed Noreldin; Norhan Elshony; Hamida Saleh; Yaser H. A. Elewa; Shaimaa M. A. Hassan; Abdullah A. Saati; Helal F. Hetta; Gaber El-Saber Batiha; Masakazu Umezawa; Hazem M. Shaheen
  SCIENTIFIC REPORTS, 11, 1, 2021年01月
  英語, 研究論文（学術雑誌）
• Unique morphological characteristics in the ovary of cotton rat (Sigmodon hispidus).
  Md Rashedul Islam; Osamu Ichii; Teppei Nakamura; Takao Irie; Md Abdul Masum; Marina Hosotani; Yuki Otani; Yaser Hosny Ali Elewa; Yasuhiro Kon
  The Journal of reproduction and development, 66, 6, 529, 538, 2020年12月22日, ［国内誌］
  英語, 研究論文（学術雑誌）, Cotton rats (Sigmodon hispidus, CRs) are commonly used as animal models in biomedical research. However, the reproductive characteristics and ovarian development in the CRs has not been widely investigated. We have previously shown that female CRs, in particular, show several unique phenotypes associated with the urogenital system, such as chronic kidney disease and pyometra. Our investigation revealed unique morphologies in CR ovaries, particularly in oocytes. Cotton rat ovaries at 6-8 weeks of age were obtained from the Hokkaido Institute of Public Health, and their sections analyzed by light microscopy and transmission electron microscopy. Although the general histology and folliculogenesis of CR ovaries were similar to those of other experimental rodents, multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) were also observed. Although MOFs were found at all stages of follicular development, a greater frequency of MOFs was observed in the primary and secondary stages. However, DNOs tended to be frequently observed in primordial follicles. Almost all MOF oocytes and a few DNOs possessed a clear zona pellucida, expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and Forkhead box protein 2, a representative marker of oocytes and follicular epithelial cells. Thus, our investigations revealed the unique phenotypes of the CR ovary. As MOFs and DNOs are occasionally observed in human patients with infertility, the CR would be a useful animal model to study for gaining a better understanding of folliculogenesis and oocytogenesis, as well as their abnormalities in humans and other animals.
• Comparative Features of the Upper Alimentary Tract in the Domestic Fowl (Gallus gallus domesticus) and Kestrel (Falco tinnunculus): A Morphological, Histochemical, and Scanning Electron Microscopic Study
  Abdallah A. Alsanosy; Ahmed E. Noreldin; Yaser H. A. Elewa; Sahar F. Mahmoud; Mohamed A. Elnasharty; Asmaa Aboelnour
  Microscopy and Microanalysis, 27, 1, 201, 214, Oxford University Press (OUP), 2020年12月10日
  研究論文（学術雑誌）, Abstract
  
  The avian alimentary tract has evolved into different histologic structures to accommodate the physical and chemical features of several food types and flight requirements. We compared the esophagus, proventriculus, and gizzard of the domestic fowl,Gallus gallus domesticus(GGD) and kestrels,Falco tinnunculus(FT) using immunohistochemistry and scanning electron microscopy with various stains and lectins [Dolichos biflorusagglutinin (DBA) andRicinus communisagglutinin I (RCA₁₂₀)], andα-smooth muscle actin (α-SMA). The esophagus of GGD demonstrated thickened epithelium, muscularis mucosae, and inner circular longitudinal tunica muscularis layers; moderate outer longitudinal tunica muscularis layers; and a true crop. In contrast, the esophagus of FT showed a thin epithelium, no muscularis mucosae, moderate inner longitudinal and thick outer circular tunica muscularis layers, and no true crop. In the proventriculus, the nature of the secretion in GGD was neutral, but that of FT was acidic and neutral. In the gizzard, the muscle coat of GGD byα-SMA had no muscularis mucosae, unlike FT, which had muscularis mucosae. In summary, there are many histologic differences between GGD and FT to meet their different physiologic needs, such as feeding.
• Hypoxia-Preconditioned Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Mitigate Hypoglycemic Testicular Injury Induced by Insulin in Rats
  Arigue A. Dessouky; Zienab A. Gouda; Mona A.A. Arafa; Yaser H.A. Elewa; Amany M. Abo-Ouf; Eman M. Askar
  Cells Tissues Organs, 209, 2-3, 83, 100, S. Karger AG, 2020年10月28日
  研究論文（学術雑誌）, Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypoxia-preconditioned human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on hypoglycemic testicular injury. We examined 56 testes from 28 animals: 7 rats with insulin-induced hypoglycemia (HG group), 7 hypoglycemic rats which received an intratesticular injection of hUCB-MSCs (HG-MSC group), and 14 untreated control rats. Testosterone level, testicular catalase (CAT) activity, and malondialdehyde (MDA) level were analyzed. Immunostaining for specific testicular germ and somatic cell markers was performed. Proliferating and apoptotic cells were detected by anti-PCNA and anti-caspase-3, respectively. Morphometrical data were statistically analyzed. The hypoglycemic rats showed a significant decrease in testosterone level and CAT activity and a significant increase in MDA production. Examination of histological structure and protein expression of diverse germ cell markers revealed collapsed tubules that were lined by degenerated germ cells, decreased lactate dehydrogenase type C immune expression, as well as decreased proliferating and increased apoptotic cells number in hypoglycemic testes. Injection of MSCs improved testicular biochemical parameters, preserved germ cells and somatic cells, and decreased apoptosis. In conclusion, hypoxia-preconditioned hUCB-MSCs attenuate rat testicular injury caused by insulin-induced hypoglycemia. Avoidance and rapid management of hypoglycemia are necessary to avoid significant testicular injury.
• Chemo-Protective Potential of Cerium Oxide Nanoparticles against Fipronil-Induced Oxidative Stress, Apoptosis, Inflammation and Reproductive Dysfunction in Male White Albino Rats
  Hamida Saleh; Atef M. K. Nassar; Ahmed E. Noreldin; Dalia Samak; Norhan Elshony; Lamiaa Wasef; Yaser H. A. Elewa; Shaimaa M. A. Hassan; Abdullah A. Saati; Helal F. Hetta; Gaber El-Saber Batiha; Masakazu Umezawa; Hazem M. Shaheen; Yasser S. El-Sayed
  MOLECULES, 25, 15, 2020年08月
  英語, 研究論文（学術雑誌）
• Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats
  Ali H. El-Far; Mohamed A. Lebda; Ahmed E. Noreldin; Mustafa S. Atta; Yaser H. A. Elewa; Mohamed Elfeky; Shaker A. Mousa
  International Journal of Molecular Sciences, 21, 12, 4348, 4348, MDPI AG, 2020年06月18日
  研究論文（学術雑誌）, Aging is an oxidative stress-associated process that progresses with age. Our aim is to delay or attenuate these oxidative alterations and to keep individuals healthy as they age using natural compounds supplementation. Therefore, we conducted the present study to investigate the protective potentials of quercetin against D-galactose (D-gal)-associated oxidative alterations that were induced experimentally in male Wistar rats. Forty-five rats were randomly allocated into five groups of nine rats each. The groups were a control group that was reared on a basal diet and injected subcutaneously with 120 mg D-gal dissolved in physiological saline solution (0.9% NaCl) per kg body weight daily and quercetin-treated groups that received the same basal diet and subcutaneous daily D-gal injections were supplemented orally with 25, 50, and 100 mg of quercetin per kg body weight for 42 days. Pancreatic and renal samples were subjected to histopathological, immunohistochemical, and relative mRNA expression assessments. Aging (p53, p21, IL-6, and IL-8), apoptotic (Bax, CASP-3, and caspase-3 protein), proliferative (Ki67 protein), antiapoptotic (Bcl2 and Bcl2 protein), inflammatory (NF-κB, IL-1β, and TNF-α), antioxidant (SOD1), and functional markers (GCLC and GCLM genes and insulin, glucagon, and podocin proteins) were determined to evaluate the oxidative alterations induced by D-gal and the protective role of quercetin. D-gal caused oxidative alterations of the pancreas and kidneys observed via upregulations of aging, apoptotic, and inflammatory markers and downregulated the antiapoptotic, proliferative, antioxidant, and functional markers. Quercetin potentially attenuated these aging-related oxidative alterations in a dose-dependent manner. Finally, we can conclude that quercetin supplementation is considered as a promising natural protective compound that could be used to delay the aging process and to maintain human health.
• BXSB/MpJ-Yaa mouse model of systemic autoimmune disease shows increased apoptotic germ cells in stage XII of the seminiferous epithelial cycle
  Yuki Otani; Osamu Ichii; Md. Abdul Masum; Junpei Kimura; Teppei Nakamura; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and Tissue Research, 381, 1, 203, 216, 2020年04月04日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review.
  Gaber El-Saber Batiha; Amany Magdy Beshbishy; Lamiaa G Wasef; Yaser H A Elewa; Ahmed A Al-Sagan; Mohamed E Abd El-Hack; Ayman E Taha; Yasmina M Abd-Elhakim; Hari Prasad Devkota
  Nutrients, 12, 3, 2020年03月24日, ［国際誌］
  英語, 研究論文（学術雑誌）, Medicinal plants have been used from ancient times for human healthcare as in the form of traditional medicines, spices, and other food components. Garlic (Allium sativum L.) is an aromatic herbaceous plant that is consumed worldwide as food and traditional remedy for various diseases. It has been reported to possess several biological properties including anticarcinogenic, antioxidant, antidiabetic, renoprotective, anti-atherosclerotic, antibacterial, antifungal, and antihypertensive activities in traditional medicines. A. sativum is rich in several sulfur-containing phytoconstituents such as alliin, allicin, ajoenes, vinyldithiins, and flavonoids such as quercetin. Extracts and isolated compounds of A. sativum have been evaluated for various biological activities including antibacterial, antiviral, antifungal, antiprotozoal, antioxidant, anti-inflammatory, and anticancer activities among others. This review examines the phytochemical composition, pharmacokinetics, and pharmacological activities of A. sativum extracts as well as its main active constituent, allicin.
• The Pharmacological Activity, Biochemical Properties, and Pharmacokinetics of the Major Natural Polyphenolic Flavonoid: Quercetin.
  Gaber El-Saber Batiha; Amany Magdy Beshbishy; Muhammad Ikram; Zohair S Mulla; Mohamed E Abd El-Hack; Ayman E Taha; Abdelazeem M Algammal; Yaser Hosny Ali Elewa
  Foods (Basel, Switzerland), 9, 3, 2020年03月23日, ［国際誌］
  英語, 研究論文（学術雑誌）, Flavonoids are a class of natural substances present in plants, fruits, vegetables, wine, bulbs, bark, stems, roots, and tea. Several attempts are being made to isolate such natural products, which are popular for their health benefits. Flavonoids are now seen as an essential component in a number of cosmetic, pharmaceutical, and medicinal formulations. Quercetin is the major polyphenolic flavonoid found in food products, including berries, apples, cauliflower, tea, cabbage, nuts, and onions that have traditionally been treated as anticancer and antiviral, and used for the treatment of allergic, metabolic, and inflammatory disorders, eye and cardiovascular diseases, and arthritis. Pharmacologically, quercetin has been examined against various microorganisms and parasites, including pathogenic bacteria, viruses, and Plasmodium, Babesia, and Theileria parasites. Additionally, it has shown beneficial effects against Alzheimer's disease (AD), and this activity is due to its inhibitory effect against acetylcholinesterase. It has also been documented to possess antioxidant, antifungal, anti-carcinogenic, hepatoprotective, and cytotoxic activity. Quercetin has been documented to accumulate in the lungs, liver, kidneys, and small intestines, with lower levels seen in the brain, heart, and spleen, and it is extracted through the renal, fecal, and respiratory systems. The current review examines the pharmacokinetics, as well as the toxic and biological activities of quercetin.
• Altered ciliary morphofunction in the oviductal infundibulum of systemic autoimmune disease-prone MRL/MpJ-Faslpr/lpr mice
  Hosotani M; Ichii O; Nakamura T; Masum MA; Otani Y; Elewa YHA; Kon Y
  Cell and Tissue Research, 380, 3, 627, 641, 2020年02月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, According to our previous reports, impaired oocyte pickup was observed in the oviductal infundibulum of an autoimmune disease (AD) mouse model, suggesting a relationship between female infertility and AD. This study examines the relationship between AD and infundibulum morphofunction by focusing on the epithelial cilia. Healthy MRL/MpJ and AD-prone MRL/MpJ-Faslpr/lpr mice were examined at 3 and 6 months of age, representing early and late disease stages, respectively. Oocyte pickup indices decreased with AD progression indicated by splenomegaly, autoantibody production and increased T cell counts of infundibulum mucosa in MRL/MpJ-Faslpr/lpr mice. Ciliary beating frequency (CBF) and height in the infundibulum were faster and higher in MRL/MpJ-Faslpr/lpr mice than in MRL/MpJ mice at the early AD stages, although the absolute CBF values were lower at the late AD stage. At the late stage, ciliary height did not differ between mouse lines but the morphological index of cilia beating direction indicated randomized patterns in MRL/MpJ-Faslpr/lpr mice. The tracheal mucosa was also examined as a representative example of cilia morphology; its CBF decreased at the late AD stage in MRL/MpJ-Faslpr/lpr; however, there were no AD-related morphological changes. Our results demonstrate altered cilia motility in systemic and reproductive organs, with such morphological changes of the infundibulum likely impairing function, including oocyte pickup.
• Prevalence, virulence attributes, and antibiogram of Bordetella avium isolated from turkeys in Egypt
  Eldin, W.F.S.; Abd-El Samie, L.K.; Darwish, W.S.; Elewa, Y.H.A.
  Tropical Animal Health and Production, 52, 1, 2020年
  研究論文（学術雑誌）
• Histopathological changes in tear-secreting tissues and cornea in a mouse model of autoimmune disease
  Hiraishi, M.; Masum, M.A.; Namba, T.; Otani, Y.; Elewa, Y.H.A.; Ichii, O.; Kon, Y.
  Experimental Biology and Medicine, 245, 12, 2020年
  研究論文（学術雑誌）
• Stannius Corpuscles in African Catfish (Clarias gariepinus): Histological and Ultrastructure Studies
  Mayada Wahid Karkit; Yaser Hosny Ali Elewa; Hoda Foad Salem; Mohammad Hafez Bareedy
  Advances in Animal and Veterinary Sciences, 7, 6, 11, Nexus Academic Publishers, 2020年
  英語, 研究論文（学術雑誌）
• Aging-associated immunosenescence via alterations in splenic immune cell populations in rat
  Nesma I. El-naseery; Hanaa S.E. Mousa; Ahmed E. Noreldin; Ali H. El-Far; Yaser H.A. Elewa
  Life Sciences, 241, 117168, 117168, Elsevier BV, 2020年01月
  研究論文（学術雑誌）
• Unique Running Pattern and Mucosal Morphology Found in the Colon of Cotton Rats.
  Tsolmon Chuluunbaatar; Osamu Ichii; Teppei Nakamura; Takao Irie; Takashi Namba; Md Rashedul Islam; Yuki Otani; Md Abdul Masum; Yuko Okamatsu-Ogura; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in physiology, 11, 587214, 587214, 2020年, ［国際誌］
  英語, 研究論文（学術雑誌）, Cotton rats are one of the experimental rodents used for testing different infectious and non-infectious diseases, including gastrointestinal tract pathology. However, their intestinal morphological characteristics are still poorly understood. Here, we clarified the anatomical and histological characteristics of the cecum and ascending colon (AC) of young (1-3-month old), adult (4-6-month old), and old (10-12-month old) cotton rats. The large intestine (LI) in cotton rats is composed of the cecum, AC, transverse and descending colons, and rectum, and is similar to that of other mammals. The AC begins with a double or triple spiral loop-like flexure (SLLF) and ends with a coupled horseshoe-like flexure (HSLF). A single longitudinal mucosal fold (SLMF) was found at the beginning of the AC along the mesentery line and developed with age. Furthermore, the SLMF contained several lymphatic nodules (LNs), indicating their role in digestive and immunological functions. Small and large protuberant LNs were found in the cecum and SLLF, respectively, whereas thin and flat LNs were observed in the HSLF and transverse colon, respectively. Regarding sex-related differences, adult females had a significantly longer AC with a higher number of SLLFs compared to males. The SLMF length and LN number were also longer and higher, respectively, in adult females compared to adult males. These are crucial findings, indicating the presence of sex-related differences in the morphology of the LI in cotton rats, and ours is the first study to discover a sex difference in the mammalian LI lining. Our study clarified the unique morphology of the LI in cotton rats, which could serve as the principal model for elucidating species-specific digestive tract functions and gastrointestinal disorders.
• Vasculature-Associated Lymphoid Tissue: A Unique Tertiary Lymphoid Tissue Correlates With Renal Lesions in Lupus Nephritis Mouse Model.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Yuki Otani; Takashi Namba; Yasuhiro Kon
  Frontiers in immunology, 11, 595672, 595672, 2020年, ［国際誌］
  英語, 研究論文（学術雑誌）, Lupus nephritis (LN) is a common complication in young patients and the most predominant cause of glomerulonephritis. Infiltrating immune cells and presence of immunocomplexes in the kidney are hallmarks of LN, which is closely associated with renal lesions (RLs). However, their regulatory mechanism in the kidney remains unclear, which is valuable for prevention of RL development. Here, we show the development of vasculature-associated lymphoid tissue (VALT) in LN, which is related to renal inflammatory cytokines, indicating that VALT is a unique tertiary lymphoid tissue. Transcriptomic analysis revealed different chemokines and costimulatory molecules for VALT induction and organization. Vascular and perivascular structures showed lymphoid tissue organization through lymphorganogenic chemokine production. Transcriptional profile and intracellular interaction also demonstrated antigen presentation, lymphocyte activity, clonal expansion, follicular, and germinal center activity in VALT. Importantly, VALT size was correlated with infiltrating immune cells in kidney and RLs, indicating its direct correlation with the development of RLs. In addition, dexamethasone administration reduced VALT size. Therefore, inhibition of VALT formation would be a novel therapeutic strategy against LN.
• Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus).
  Md Rashedul Islam; Osamu Ichii; Teppei Nakamura; Takao Irie; Md Abdul Masum; Yuki Otani; Takashi Namba; Tsolmon Chuluunbaatar; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in physiology, 11, 601927, 601927, 2020年, ［国際誌］
  英語, 研究論文（学術雑誌）, The reproductive characteristics and ovarian development in cotton rats (Sigmodon hispidus, CRs) are unclear, although CRs are commonly used as animal models in biomedical research. We previously reported that young (6-8 weeks) CRs showed multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) in different stages of follicles. The developmental changes in neonatal CR ovaries were investigated in the present study and were compared with our findings in previous studies of unique phenotypes, particularly in oocytes. CR ovaries at postnatal days (PND) 0, 4, and 7 were obtained from the Hokkaido Institute of Public Health. Samples were analyzed by light and transmission electron microscopy. The general histology and folliculogenesis in CR ovaries were similar to those in other experimental rodents. However, DNOs were observed in all age categories and were frequently observed in primordial follicles, whereas MOFs started to develop from PND4 with greater frequency in primary follicles. Almost all developing follicles expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and forkhead box L2, which are representative markers of oocytes and follicular epithelial cells, respectively. Ki-67 staining demonstrated the proliferative activity of granulosa cells, but not of oocytes, in follicles. Moreover, rapid folliculogenesis of CR due to a small number of apoptotic oocytes was suggested, based on results of the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, confirming the formation of DNOs or MOFs. These findings clarify the development of unique phenotypes of neonatal CR ovaries and support it as a useful model to better understand folliculogenesis and oocytogenesis as well as their abnormalities in humans and other animals.
• Podocyte Injury Through Interaction Between Tlr8 and Its Endogenous Ligand miR-21 in Obstructed and Its Collateral Kidney.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Frontiers in immunology, 11, 606488, 606488, 2020年, ［国際誌］
  英語, 研究論文（学術雑誌）, While chronic kidney disease is prevalent in adults, obstructive nephropathy (ON) has been reported in both young and old patients. In ON, tubulointerstitial lesions (TILs) have been widely investigated, but glomerular lesions (GLs) have been largely neglected. Here, we show a novel mechanism underlying GL development in ON in young and old mice. TILs develop earlier than GLs owing to infiltration of inflammatory cells in the tubulointerstitium, but GLs develop following the activation of Toll-like receptor 8 (Tlr8) even though the absence of inflammatory cells infiltrating the glomerulus. TLR8 and interleukin 1 beta (IL1β) proteins colocalize with reducing podocyte function markers (PFMs), indicating the activation of TLR8 signaling in injured podocytes. Furthermore, glomerular and serum levels of miR-21, an endogenous ligand for Tlr8, were higher in the ON mouse model than in the sham control. The glomerular expression of Tlr8 positively correlates with miR-21 and the downstream cytokines Il1b and Il6 and negatively correlated with PFMs (Nphs1 and Synpo). We also show the colocalization of TLR8 and IL1β proteins with reducing PFMs in both obstructed and collateral kidney of young and old mice. Furthermore, in vitro study results revealed higher expression of Tlr8 and its downstream cytokines in glomeruli from obstructed kidneys following treatment with miR-21 mimic than in the control. In conclusion, the overexpression of Tlr8 may serve as a plausible mechanism underlying GL development in ON through podocyte injury.
• Spleen selective enhancement of transfection activities of plasmid DNA driven by octaarginine and an ionizable lipid and its implications for cancer immunization
  Seigo Kimura; Ikramy A. Khalil; Yaser H.A. Elewa; Hideyoshi Harashima
  Journal of Controlled Release, 313, 70, 79, Elsevier BV, 2019年11月
  研究論文（学術雑誌）
• Age-related changes in bone morphology, function, and cell populations in inbred C57BL/6N mice
  Takashi Namba; Osamu Ichii; Teppei Nakamura; Md Abdul Masum; Yuki Otani; Saori Otsuka-Kanazawa; Yaser Hosny Ali Elewa; Yasuhiro Kon
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 67, 4, 313, 317, 2019年11月
  英語, 研究論文（学術雑誌）
• Age-related glomerular lesions with albuminuria in male cotton rats.
  Osamu Ichii; Teppei Nakamura; Takao Irie; Yuki Otani; Marina Hosotani; Md Abdul Masum; Rashedul Md Islam; Taro Horino; Yuji Sunden; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Histochemistry and cell biology, 153, 1, 27, 36, 2019年10月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, The increased prevalence of aging-related chronic kidney disease (CKD) among humans is a problem worldwide. Aged cotton rats (Sigmodon hispidus) are considered novel model animals for studying CKD, especially as the females develop severe tubulointerstitial lesions with anemia. To investigate the renal pathologic features in aged male cotton rats and their characteristic glomerular injuries, the animals were divided into young, adult, old-aged, and advanced-aged groups (1-4, 5-8, 9-12, and 13-17 months, respectively) and pathologically analyzed. Anemia and renal dysfunction, as indicated by hematologic and serologic parameters, were significantly milder in the advanced-aged males than in the old-aged females. The males had increased urinary albumin-to-creatinine ratios from the old-age period, with the advanced-aged males having significantly higher levels than those in the old-aged females and young males. The old-aged females did not show clear glomerular injuries, whereas the advanced-aged males showed membranous lesions characterized by irregular and thickened glomerular basement membranes (GBMs). Characteristically, several large-sized projections from the GBM toward the podocytes were observed by microscopy, and podocytes covering these projections effaced their foot processes. The advanced-aged males showed aging-related IgG immune-complex depositions in the paramesangial regions and along the GBM. Furthermore, the positive reaction for podocin (a podocyte molecule) was granulated along the GBM. Thus, we clarified the albuminuria associated with altered glomerular structures in advanced-aged cotton rats, and that these phenotypes were closely associated with aging. These data help to clarify the aging-related pathogenesis of glomerular injury.
• Profiling of cellular immune responses to Mycoplasma pulmonis infection in C57BL/6 and DBA/2 mice
  Boonyarattanasoonthorn Tussapon; Elewa Yaser; Hosny Ali; Tag-El-Din-Hassan; Hassan T; Morimatsu Masami; Agui Takashi
  INFECTION GENETICS AND EVOLUTION, 73, 55, 65, 2019年09月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Slc:Wistar/ST rats develop unilateral thyroid dysgenesis: A novel animal model of thyroid hemiagenesis
  Teppei Nakamura; Michael Schubert; Osamu Ichii; Yuji Sunden; Yaser Hosny Ali Elewa; Tomoji Yoshiyasu; Hideki Hattori; Osamu Tatsumi; Yasuhiro Kon; Ken-ichi Nagasaki
  PLOS ONE, 14, 8, e0221939, 2019年08月29日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Immune-associated renal disease found in caspase 3-deficient mice
  Takashi Suzuki; Osamu Ichii; Teppei Nakamura; Taro Horino; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Cell and Tissue Research, 379, 2, 323, 335, 2019年08月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Histopathological features of the proper gastric glands in FVB/N-background mice carrying constitutively-active aryl-hydrocarbon receptor
  Ai Dantsuka; Osamu Ichii; Annika Hanberg; Yaser Hosny Ali Elewa; Saori Otsuka-Kanazawa; Teppei Nakamura; Yasuhiro Kon
  BMC Gastroenterology, 19, 1, 102, 102, Springer Science and Business Media {LLC}, 2019年06月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, BACKGROUND: Aryl-hydrocarbon receptor (AhR) is a multiple ligand-activated transcription factor that has important roles in xenobiotic, physiological, or pathological functions. Transgenic mice systemically expressing constitutively-active AhR (CA-AhR) have been created to mimic activated AhR signaling in vivo. However, their detailed histopathological features are unclear. In the present study, we generated CA-AhR-expressing FVB/N mice (FVB-CA-AhR mice) and clarified their phenotypes in detail. METHODS: Male and female FVB-CA-AhR and wild-type mice were histopathologically examined from 6 to 33 weeks of age. RESULTS: Among the systemic organs, only the stomachs in FVB-CA-AhR mice showed pathological changes including cystic structures beneath the serosa; in addition, stomach weights increased with age. Histopathologically, cystic structures and alcian blue-positive metaplasia were observed in the mucosa of the proper gastric glands, and these two histometric parameters were positively correlated. Furthermore, proliferating cells shifted from the isthmus to the base of the glands, and parietal cells decreased. Age-related histopathological changes were clearer in females than in males. Importantly, in FVB-CA-AhR mice, intramucosal cysts developed as extramucosal cysts beneath the serosa, penetrating the lamina muscularis mucosae and the muscularis propria. Their incidence reached 100% in 28-week-old male mice and 33-week-old female mice. Extramucosal cysts contained alcian blue-, Griffonia simplicifolia lectin II-, or trefoil factor 2-positive cells, suggesting a stomach origin for the cysts and spasmolytic polypeptide-expressing metaplasia-like lesions. CONCLUSIONS: Disease onset occurred earlier in FVB-CA-AhR mice than previously reported in C57BL/6-derived CA-AhR mice. Importantly, the histopathological features were partly similar with gastritis cystica profunda in humans and animals. Excessive activation of AhR signaling aggravated abnormalities in the gastric mucosa and were affected by both genetic- and sex-related factors.
• Lung-Endothelium-Targeted Nanoparticles Based on a pH-Sensitive Lipid and the GALA Peptide Enable Robust Gene Silencing and the Regression of Metastatic Lung Cancer
  Mahmoud M. Abd Elwakil; Ikramy A. Khalil; Yaser H. A. Elewa; Kenji Kusumoto; Yusuke Sato; Nour Shobaki; Yasuhiro Kon; Hideyoshi Harashima
  Advanced Functional Materials, 29, 18, Wiley-VCH Verlag, 2019年05月02日
  英語, 研究論文（学術雑誌）
• Feature Article: Altered morpho-functional features of bones in autoimmune disease-prone BXSB/MpJ-Yaa mice
  Takashi Namba; Osamu Ichii; Teppei Nakamura; Md Abdul Masum; Yuki Otani; Saori Otsuka-Kanazawa; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Experimental Biology and Medicine, 244, 5, 153537021983281, 153537021983281, 2019年04月28日, ［査読有り］
  英語, 研究論文（学術雑誌）
• MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment
  Hosotani, M.; Ichii, O.; Nakamura, T.; Masum, M.A.; Otani, Y.; Otsuka-Kanazawa, S.; Elewa, Y.H.A.; Kon, Y.
  Reproduction, Fertility and Development, 31, 4, 760, 773, 2019年04月, ［査読有り］
  研究論文（学術雑誌）
• Food Yellow4 reprotoxicity in relation to localization of DMC1 and apoptosis in rat testes: Roles of royal jelly and cod liver oil.
  Yaser H A Elewa; Amany Abdel-Rahman Mohamed; Azza A A Galal; Nesma I El-Naseery; Osamu Ichii; Yasuhiro Kon
  Ecotoxicology and environmental safety, 169, 696, 706, 2019年03月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, Food Yellow 4 (FY4) is a lemon-yellow-colored synthetic organic azo dye, which is used widely for imparting pleasant and attractive appearance to foods and cosmetics. The present study aimed at evaluating the possible mechanism underlying the FY4-induced reprotoxicity in rats, and the potential supportive role of royal jelly (RJ) or cod liver oil (CLO), which is a natural remedy with several pharmacological benefits, against induced toxicity. Forty-eight male rats were divided into different groups-the control group, the CLO group (0.4 mL/kg), the RJ group (300 mg/kg), the FY4 group (500 mg/kg b.w.), and the co-treated groups (FY4 + CLO or FY4 + RJ). Semen analysis, serum hormones, and enzyme activities were estimated. Immunohistochemical staining was performed using anti-PCNA, anti-Sox 9, anti-STRA8, anti-DMC1, and anti-ssDNA antibody. The FY4 group exhibited a significant decrease in sperm concentration and motility percentage (%) and a substantial reduction in the TES and LH levels. Testicular LDH, ACP, and SDH were observed to be inhibited. Furthermore, co-localization of DMC1 and ssDNA, which reflected apoptotic induction in the leptotene and zygotene spermatocytes, respectively, was observed to have markedly elevated in the FY4 treated rats, with fewer PCNA-positive and SOX9-positive cells and higher ssDNA-positive cells in the seminiferous epithelium in comparison to the control groups. Interestingly, co-treatment with CLO or RJ exhibited healthy sperms and restored their features, activated the enzyme production, and raised the levels of sexual hormones. In addition, both RJ and CLO restored the features of the testicular tissue as observed under a light microscope, and limited the apoptosis as observed through antibody staining. Collectively, the results of the present study revealed that the co-administration of RJ or CLO with FY4 improved the biochemical, hormonal, and structural aspects of the testicular tissue in rats. Therefore, CLO and RJ may be considered promising agents that would be able to improve the testicular structure and function in the FY4-exposed individuals.
• Induced expression of Toll-like receptor 9 in peritubular capillary endothelium correlates with the progression of tubulointerstitial lesions in autoimmune disease-prone mice.
  M A Masum; O Ichii; Y H A ELewa; Y Kon
  Lupus, 28, 3, 324, 333, 2019年03月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, BACKGROUND: Toll-like receptor (Tlr) 9 is capable of recognizing exogenous and/or endogenous nucleic acids and plays a crucial role in innate and adaptive immunity. Recently, we showed that Tlr9 is overexpressed in podocytes, a component of the blood-urine barrier (BUB), in glomeruli of autoimmune glomerulonephritis (AGN) model mice. This study investigated the activation of peritubular capillary (PTC) endothelial cells (ECs), a component of the BUB in the tubulointerstitium, through overexpressing Tlr9, and the subsequent development of tubulointerstitial lesions (TILs) in AGN model mice. METHODS: Lupus-prone BXSB/MpJ-Yaa (Yaa) and BXSB/MpJ (BXSB) mice were used as an AGN model and control, respectively. In addition to histopathological and ultrastructural techniques, protein and mRNA levels were also evaluated. The relationship between Tlr9 and TIL indices was analyzed by statistical correlation analysis. RESULTS: Yaa mice developed TILs and showed strong Tlr9 mRNA expression in PTC ECs at 24 weeks (wks) of age. However, BXSB mice showed no TIL but faint expression of Tlr9 mRNA at 8 and 24 wks of age. Tlr9 protein localization on PTC was almost absent in BXSB mice at both ages but intense expression was found in Yaa mice only at 24 wks of age. Relative mRNA expression of Tlr9 and its putative downstream cytokines, including interleukin 1 beta ( Il1b), Il6, interferon gamma ( Ifng), and tumor necrosis factor alpha ( Tnf) was markedly increased in isolated tubulointerstitium from Yaa mice at 24 wks of age. Furthermore, electron microscopy examination revealed PTC injury and TIL in Yaa mice at 24 wks. The expression level of Tlr9 in the tubulointerstitium was correlated with inflammatory cells in TILs, injured PTC, Ilb and Tnf expression, and damaged tubules ( P < 0.05 and 0.01). CONCLUSION: Induced expression of Tlr9 in ECs correlates with PTC injury and the development of TILs in lupus-prone AGN model mice.
• Large expert-curated database for benchmarking document similarity detection in biomedical literature search
  Brown, P.; Zhou, Y.; Tan, A.-C.; El-Esawi, M.A.; Liehr, T.; Blanck, O.; Gladue, D.P.; Almeida, G.M.F.; Cernava, T.; Sorzano, C.O.; Yeung, A.W.K.; Engel, M.S.; d Chandrasekaran, A.R.; Muth, T.; Staege, M.S.; Daulatabad, S.V.; Widera, D.; Zhang, J.; Meule, A.; Honjo, K.; Pourret, O.; Yin, C.-C.; Zhang, Z.; Cascella, M.; Flegel, W.A.; Goodyear, C.S.; van Raaij, M.J.; Bukowy-Bieryllo, Z.; Campana, L.G.; Kurniawan, N.A.; Lalaouna, D.; H{\"u}ttner, F.J.; Ammerman, B.A.; Ehret, F.; Cobine, P.A.; Tan, E.-C.; Han, H.; Xia, W.; McCrum, C.; Dings, R.P.M.; Marinello, F.; Nilsson, H.; Nixon, B.; Voskarides, K.; Yang, L.; Costa, V.D.; Bengtsson-Palme, J.; Bradshaw, W.; Grimm, D.G.; Kumar, N.; Martis, E.; Prieto, D.; Sabnis; S.C. and Amer; S.E.D.R.; d Liew; A.W.C.; Perco, P.; Rahimi, F.; Riva, G.; Zhang, C.; Devkota, H.P.; Ogami, K.; Basharat, Z.; Fierz, W.; Siebers, R.; Tan, K.H.; Boehme, K.A.; Brenneisen, P.; Brown, J.A.L.; Dalrymple, B.P.; Harvey, D.J.; Ng, G.; Werten, S.; Bleackley, M.; Dai, Z.; Dhariwal, R.; Gelfer, Y.; Hartmann, M.D.; Miotla, P.; Tamaian, R.; Govender, P.; Gurney-Champion, O.J.; Kauppila, J.H.; Zhang, X.; Echeverr{\'i}a, N.; Subhash, S.; Sallmon, H.; Tofani, M.; Bae, T.; Bosch, O.; Cu{\'i}v, P.O.; Danchin, A.; Diouf, B.; Eerola, T.; Evangelou, E.; Filipp, F.; Klump, H.; Kurgan, L.; Smith, S.S.; Terrier, O.; Tuttle, N.; Ascher, D.B.; Janga, S.C.; Schulte, L.N.; Becker, D.; Browngardt, C.; Bush, S.J.; Gaullier, G.; Ide, K.; Meseko, C.; Werner, G.D.A.; Zaucha, J.; Al-Farha, A.A.; Greenwald, N.F.; Popoola, S.I.; Rahman, S.; Xu, J.; Yang, S.Y.; Hiroi, N.; Alper, O.M.; Baker, C.I.; Bitzer, M.; Chacko, G.; Debrabant, B.; Dixon, R.; Forano, E.; Gilliham, M.; Kelly, S.; Klempnauer, K.-H.; Lidbury, B.A.; Lin, M.Z.; Lynch, I.; Ma, W.; Maibach, E.W.; Mather, D.E.; Nandakumar, K.S.; Ohgami, R.S.; Parchi, P.; Tressoldi, P.; Xue, Y.; Armitage, C.; Barraud, P.; Chatzitheochari, S.; Coelho, L.P.; Diao, J.; Doxey, A.C.; Gobet, A.; Hu, P.; Kaiser, S.; Mitchell, K.M.; Salama, M.F.; Shabalin, I.G.; Song, H.; Stevanovic, D.; Yadollahpour, A.; Zeng, E.; Zinke, K.; Alimba, C.G.; Beyene, T.J.; Cao, Z.; Chan, S.S.; Gatchell, M.; Kleppe, A.; Piotrowski, M.; Torga, G.; Woldesemayat, A.A.; Cosacak, M.I.; Haston, S.; Ross, S.A.; Williams, R.; Wong, A.; Abramowitz, M.K.; Effiong, A.; Lee, S.; Abid, M.B.; Agarabi, C.; Alaux, C.; Albrecht, D.R.; Atkins, G.J.; Beck, C.R.; Bonvin, A.M.J.J.; Bourke, E.; Brand, T.; Braun, R.J.; Bull, J.A.; Cardoso, P.; Carter, D.; Delahay, R.M.; Ducommun, B.; Duijf, P.H.G.; Epp, T.; Eskelinen, E.-L.; Fallah, M.; Farber, D.B.; Fernandez-Triana, J.; Feyerabend, F.; Florio, T.; Friebe, M.; Furuta, S.; Gabrielsen, M.; Gruber, J.; Grybos, M.; Han, Q.; Heinrich, M.; Helanter{\"a}, H.; Huber, M.; Jeltsch, A.; Jiang, F.; Josse, C.; Jurman, G.; Kamiya, H.; de Keersmaecker, K.; Kristiansson, E.; de Leeuw, F.-E.; Li, J.; Liang, S.; Lopez-Escamez, J.A.; Lopez-Ruiz, F.J.; Marchbank, K.J.; Marschalek, R.; Mart{\'i}n, C.S.; Miele, A.E.; Montagutelli, X.; Morcillo, E.; Nicoletti, R.; Niehof, M.; O{'}Toole, R.; Ohtomo, T.; Oster, H.; Palma, J.-A.; Paterson, R.; Peifer, M.; Portilla, M.; Portillo, M.C.; Pritchard, A.L.; Pusch, S.; Raghava, G.P.S.; Roberts, N.J.; Ross, K.; Schuele, B.; Sergeant, K.; Shen, J.; Stella, A.; Sukocheva, O.; Uversky, V.N.; Vanneste, S.; Villet, M.H.; Viveiros, M.; Vorholt, J.A.; Weinstock, C.; Yamato, M.; Zabetakis, I.; Zhao, X.; Ziegler, A.; Aizat, W.M.; Atlas, L.; Bridges, K.M.; Chakraborty, S.; Deschodt, M.; Domingues, H.S.; Esfahlani, S.S.; Falk, S.; Guisado, J.L.; Kane, N.C.; Kueberuwa, G.; Lau, C.L.; Liang, D.; Liu, E.; Luu, A.M.; Ma, C.; Ma, L.; Moyer, R.; Norris, A.D.; Panthee, S.; Parsons, J.R.; Peng, Y.; Pinto, I.M.; Reschke, C.R.; Sillanp{\"a},{\"a}, E.; Stewart, C.J.; Uhle, F.; Yang, H.; Zhou, K.; Zhu, S.; Ashry, M.; Bergsland, N.; Berthold, M.; Chen, C.-E.; Colella, V.; Cuypers, M.; Eskew, E.A.; Fan, X.; Gajda, M.; Gonz{\'a}lezlez-Prendes, R.; Goodin, A.; Graham, E.B.; Groen, E.J.N.; Guti{\'e}rrez-Sacrist{\'a}n, A.; Habes, M.; Heffler, E.; Higginbottom, D.B.; Janzen, T.; Jayaraman, J.; Jibb, L.A.; Jongen, S.; Kinyanjui, T.; Koleva-Kolarova, R.G.; Li, Z.; Liu, Y.-P.; Lund, B.A.; Lussier, A.A.; Ma, L.; Mier, P.; Moore, M.D.; Nagler, K.; Orme, M.W.; Pearson, J.A.; Prajapati, A.S.; Saito, Y.; Tr{\"o}der, S.E.; Uchendu, F.; Verloh, N.; Voutchkova, D.D.; Abu-Zaid, A.; Bakkach, J.; Baumert, P.; Dono, M.; Hanson, J.; Herbelet, S.; Hobbs, E.; Kulkarni, A.; Kumar, N.; Liu, S.; Loft, N.D.; Reddan, T.; Senghore, T.; Vindin, H.; Xu, H.; Bannon, R.; Chen, B.; Cheung, J.T.K.; Cooper, J.; Esnakula, A.K.; Feghali, K.A.; Ghelardi, E.; Gnasso, A.; Horbar, J.; Lai, H.M.; Li, J.; Ma, L.; Ma, R.; Pan, Z.; Peres, M.A.; Pranata, R.; Seow, E.; Sydes, M.; Testoni, I.; Westermair, A.L.; Yang, Y.; Afnan, M.; Albiol, J.; Albuquerque, L.G.; Amir, S.; Amiya, E.; Amorim, R.M.; An, Q.; Andersen, S.U.; Aplin, J.D.; Argyropoulos, C.; Asmann, Y.W.; Assaeed, A.M.; Atanasov, A.G.; Atchison, D.A.; Avery, S.V.; Avillach, P.; Baade, P.D.; Backman, L.; Badie, C.; Baldi, A.; Ball, E.; Bardot, O.; Barnett, A.G.; Basner, M.; Batra, J.; Bazanova, O.M.; Beale, A.; Beddoe, T.; Bell, M.L.; Berezikov, E.; Berners-Price, S.; Bernhardt, P.; Berry, E.; Bessa, T.B.; Billington, C.; Birch, J.; Blakely, R.D.; Blaskovich, M.A.T.; Blum, R.; Boelaert, M.; Bogdanos, D.; Bosch, C.; Bourgoin, T.; Bouvard, D.; Boykin, L.M.; Bradley, G.; Braun, D.; Brownlie, J.; Br{\"u}hl, A.; Burt, A.; Butler, L.M.; Byrareddy, S.N.; Byrne, H.J.; Cabantous, S.; Calatayud, S.; Candal, E.; Carlson, K.; Casillas, S.; Castelvetro, V.; Caswell, P.T.; Cavalli, G.; Cerovsky, V.; Chagoyen, M.; Chen, C.-S.; Chen, D.F.; Chen, H.; Chen, H.; Chen, J.-T.; Chen, Y.; Cheng, C.; Cheng, J.; Chinapaw, M.; Chinopoulos, C.; Cho, W.C.S.; Chong, L.; Chowdhury, D.; Chwalibog, A.; Ciresi, A.; Cockcroft, S.; Conesa, A.; Cook, P.A.; Cooper, D.N.; Coqueret, O.; Corea, E.M.; Costa, A.; Costa, E.; Coupland, C.; Crawford, S.Y.; Cruz, A.D.; Cui, H.; Cui, Q.; Culver, D.C.; D{'}Angiulli, A.; Dahms, T.E.S.; Daigle, F.; Dalgleish, R.; Danielsen, H.E.; Darras, S.; Davidson, S.M.; Day, D.A.; Degirmenci, V.; Demaison, L.; Devriendt, K.; Ding, J.; Dogan, Y.; Dong, X.C.; Donner, C.F.; Dressick, W.; Drevon, C.A.; Duan, H.; Ducho, C.; Dumaz, N.; and; Dwarakanath, B.S.; Ebell, M.H.; Eisenhardt, S.; Elkum, N.; Engel, N.; Erickson, T.B.; Fairhead, M.; Faville, M.J.; Fejzo, M.S.; Festa, F.; Feteira, A.; Flood-Page, P.; Forsayeth, J.; Fox, S.A.; Franks, S.J.; Frentiu, F.D.; Frilander, M.J.; Fu, X.; Fujita, S.; Galea, I.; Galluzzi, L.; Gani, F.; Ganpule, A.P.; Garc{\'i}a-Alix, A.; Gedye, K.; Giordano, M.; Giunta, C.; Gleeson, P.A.; Goarant, C.; Gong, H.; Gora, D.; Gough, M.J.; Goyal, R.; Graham, K.E.; Grande-P{\'e}rez, A.; Graves, P.M.; Greidanus, H.; Grice, D.; Grunau, C.; Gumulya, Y.; Guo, Y.; Gurevich, V.V.; Gusev, O.; Hacker, E.; Hage, S.R.; Hagen, G.; Hahn, S.; Haller, D.M.; Hammerschmidt, S.; Han, J.; Han, R.; Handfield, M.; Hapuarachchi, H.C.; Harder, T.; Hardingham, J.E.; Heck, M.; Heers, M.; Hew, K.F.; Higuchi, Y.; Hilaire, C.S.; Hilton, R.; Hodzic, E.; Hone, A.; Hongoh, Y.; Hu, G.; Huber, H.P.; Hueso, L.E.; Huirne, J.; Hurt, L.; Idborg, H.; Ikeo, K.; Ingley, E.; Jakeman, P.M.; Jensen, A.; Jia, H.; Jia, H.; Jia, S.; Jiang, J.; Jiang, X.; Jin, Y.; Jo, D.; Johnson, A.M.; Johnston, M.; Jonscher, K.R.; Jorens, P.G.; Jorgensen, J.O.L.; Joubert, J.W.; Jung, S.-H.; Junior, A.M.; Kahan, T.; Kamboj, S.K.; Kang, Y.-K.; Karamanos, Y.; Karp, N.A.; Kelly, R.; Kenna, R.; Kennedy, J.; Kersten, B.; Khalaf, R.A.; Khalid, J.M.; Khatlani, T.; Khider, T.; Kijanka, G.S.; King, S.R.B.; Kluz, T.; Knox, P.; Kobayashi, T.; Koch, K.-W.; Kohonen-Corish, M.R.J.; Kong, X.; Konkle-Parker, D.; Korpela, K.M.; Kostrikis, L.G.; Kraiczy, P.; Kratz, H.; Krause, G.; Krebsbach, P.H.; Kristensen, S.R.; Kumari, P.; Kunimatsu, A.; Kurdak, H.; Kwon, Y.D.; Lachat, C.; Lagisz, M.; Laky, B.; Lammerding, J.; Lange, M.; Larrosa, M.; Laslett, A.L.; Laverman, G.D.; Leclair, E.E.; Lee, K.-W.; Lee, M.-Y.; Lee, M.-S.; Li, G.; Li, J.; Lieb, K.; Lim, Y.Y.; Lindsey, M.L.; Line, P.-D.; Liu, D.; Liu, F.; Liu, H.; Liu, H.; Lloyd, V.K.; Lo, T.-W.; Locci, E.; Loidl, J.; Lorenzen, J.; Lorkowski, S.; Lovell, N.H.; Lu, H.; Lu, W.; Lu, Z.; Luengo, G.S.; Lundh, L.-G.; Lysy, P.A.; Mabb, A.; Mack, H.G.; Mackey, D.A.; Mahdavi, S.R.; Maher, P.; Maher, T.; Maity, S.N.; Malgrange, B.; Mamoulakis, C.; Mangoni, A.A.; Manke, T.; Manstead, A.S.R.; Mantalaris, A.; Marsal, J.; Marschall, H.-U.; Martin, F.L.; Martinez-Raga, J.; Martinez-Salas, E.; Mathieu, D.; Matsui, Y.; Maza, E.; McCutcheon, J.E.; McKay, G.J.; McMillan, B.; McMillan, N.; Meads, C.; Medina, L.; Merrick, B.A.; Metzger, D.W.; Meunier, F.A.; Michaelis, M.; Micheau, O.; Mihara, H.; Mintz, E.M.; Mizukami, T.; Moalic, Y.; Mohapatra, D.P.; Monteiro, A.; Montes, M.; Moran, J.V.; Morozov, S.Y.; Mort, M.; Murai, N.; Murphy, D.J.; Murphy, S.K.; Murray, S.A.; Naganawa, S.; Nammi, S.; Nasios, G.; Natoli, R.M.; Nguyen, F.; Nicol, C.; van Nieuwerburgh, F.; Nilsen, E.B.; Nobile, C.J.; O{'}Mahony, M.; Ohlsson, S.; Olatunbosun, O.; Olofsson, P.; Ortiz, A.; Ostrikov, K.; Otto, S.; Outeiro, T.F.; Ouyang, S.; Paganoni, S.; Page, A.; Palm, C.; Paradies, Y.; Parsons, M.H.; Parsons, N.; Pascal, P.; Paul, E.; Peckham, M.; Pedemonte, N.; Pellizzon, M.A.; Petrelli, M.; Pichugin, A.; Pinto, C.J.C.; Plevris, J.N.; Pollesello, P.; Polz, M.; Ponti, G.; Porcelli, P.; Prince, M.; Quinn, G.P.; Quinn, T.J.; Ramula, S.; Rappsilber, J.; Rehfeldt, F.; Reiling, J.H.; Remacle, C.; Rezaei, M.; Riddick, E.W.; Ritter, U.; Roach, N.W.; Roberts, D.D.; Robles, G.; Rodrigues, T.; Rodriguez, C.; Roislien, J.; Roobol, M.J.; Rowe, A.; Ruepp, A.; van Ruitenbeek, J.; Rust, P.; Saad, S.; Sack, G.H.; Santos, M.; Saudemont, A.; Sava, G.; Schrading, S.; Schramm, A.; Schreiber, M.; Schuler, S.; Schymkowitz, J.; Sczyrba, A.; Seib, K.L.; Shi, H.-P.; Shimada, T.; Shin, J.-S.; Shortt, C.; Silveyra, P.; Skinner, D.; Small, I.; Smeets, P.A.M.; So, P.-W.; Solano, F.; Sonenshine, D.E.; Song, J.; Southall, T.; Speakman, J.R.; Srinivasan, M.V.; Stabile, L.P.; Stasiak, A.; Steadman, K.J.; Stein, N.; Stephens, A.W.; Stewart, D.I.; Stine, K.; Storlazzi, C.; Stoynova, N.V.; Strzalka, W.; Suarez, O.M.; Sultana, T.; Sumant, A.V.; Summers, M.J.; Sun, G.; Tacon, P.; Tanaka, K.; Tang, H.; Tanino, Y.; Targett-Adams, P.; Tayebi, M.; Tayyem, R.; Tebbe, C.C.; Telfer, E.E.; Tempel, W.; Teodorczyk-Injeyan, J.A.; Thijs, G.; Thorne, S.; Thrift, A.G.; Tiffon, C.; Tinnefeld, P.; Tjahjono, D.H.; Tolle, F.; Toth, E.; Del Tredici, A.L.; Tsapas, A.; Tsirigotis, K.; Turak, A.; Tzotzos, G.; Udo, E.E.; Utsumi, T.; Vaidyanathan, S.; Vaillant, M.; Valsesia, A.; Vandenbroucke, R.E.; Veiga, F.H.; Vendrell, M.; Vesk, P.A.; Vickers, P.; Victor, V.M.; Villemur, R.; Vohl, M.-C.; Voolstra, C.R.; Vuillemin, A.; Wakelin, S.; Waldron, L.; Walsh, L.J.; Wang, A.Y.; Wang, F.; Wang, Y.; Watanabe, Y.; Weigert, A.; Wen, J.-C.; Wham, C.; White, E.P.; Wiener, J.; Wilharm, G.; Wilkinson, S.; Willmann, R.; Wilson, C.; Wirth, B.; Wojan, T.R.; Wolff, M.; Wong, B.M.; Wu, T.-W.; Wuerbel, H.; Xiao, X.; Xu, D.; Xu, J.W.; Xu, J.; Xue, B.; Yalcin, S.; Yan, H.; Yang, E.-C.; Yang, S.; Yang, W.; Ye, Y.; Ye, Z.-Q.; Yli-Kauhaluoma, J.; Yoneyama, H.; Yu, Y.; Yuan, G.-C.; Yuh, C.-H.; Zaccolo, M.; Zeng, C.; Zevnik, B.; Zhang, C.; Zhang, L.; Zhang, Y.; Zhang, Y.; Zhang, Z.; Zhang, Z.-Y.; Zhao, Y.; Zhou, M.; Zuberbier, T.; Aanei, C.M.; Ahmad, R.; Al-Lawama, M.; Alanio, A.; Allardyce, J.; Alonso-Caneiro, D.; Atack, J.M.; Baier, D.; Bansal, A.; Benezeth, Y.; Berbesque, C.; Berrevoet, F.; Biedermann, P.H.W.; Bijleveld, E.; Bittner, F.; Blombach, F.; van den Bos, W.; Boudreau, S.A.; Bramoweth, A.D.; Braubach, O.; Cai, Y.; Campbell, M.; Cao, Z.; Catry, T.; Chen, X.; Cheng, S.; Chung, H.-J.; Ch{\'a}vez-Fumagalli, M.A.; Conway, A.; Costa, B.M.; Cyr, N.; Dean, L.T.; Denzel, M.S.; Dlamini, S.V.; Dudley, K.J.; Dufies, M.; Ecke, T.; Eckweiler, D.; Eixarch, E.; El-Adawy, H.; Emmrich, J.V.; Eustace, A.J.; Falter-Wagner, C.M.; Farhoudi, R.; Fuss, J.; Gao, J.; Gill, M.R.; Gloyn, L.; Goggs, R.; Govinden, U.; Greene, G.; Greiff, V.; Grundle, D.S.; Gr{\"u}neberg, P.; Gumede, N.; Haore, G.; Harrison, P.; Hoenner, X.; Hojsgaard, D.; Hori, H.; Ikonomopoulou, M.P.; Jeurissen, P.; Johnson, D.M.; Kabra, D.; Kamagata, K.; Karmakar, C.; Kasian, O.; Kaye, L.K.; Khan, M.M.; Kim, Y.-M.; Kish, J.K.; Kobold, S.; Kohanbash, G.; Kohls, G.; Kugler, J.-M.; Kumar, G.; Lacy-Colson, J.; Latif, A.; Lauschke, V.M.; Li, B.; Lim, C.J.; Liu, F.; Liu, X.; Lu, J.-J.; Lu, Q.; Mahavadi, P.; Marzocchi, U.; McGarrigle, C.A.; van Meerten, T.; Min, R.; Moal, I.; Molari, M.; Molleman, L.; Mondal, S.R.; van de Mortel, T.; Moss, W.N.; Moultos, O.A.; Mukherjee, M.; Nakayama, K.; Narayan, E.; Navaratnarajah; Neumann, P.-A.; Nie, J.; Nie, Y.; Niemeyer, F.; Nolan, F.; Nwaiwu, O.; Oldenmenger, W.H.; Olumayede, E.; Ou, J.; Pallebage-Gamarallage, M.; Pearce, S.P.; Pelkonen, T.; Pelleri, M.C.; Pereira, J.L.; Pheko, M.; Pinto, K.A.; Piovesan, A.; Pluess, M.; Podolsky, I.M.; Prescott, J.; Qi, D.; Qi, X.; Raikou, V.D.; Ranft, A.; Rhodes, J.; Rotge, J.-Y.; Rowe, A.D.; Saggar, M.; Schuon, R.A.; Shahid, S.; Shalchyan, V.; Shirvalkar, P.; Shiryayev, O.; Singh, J.; Smout, M.J.; Soares, A.; Song, C.; Srivastava, K.; Srivastava, R.K.; Sun, J.; Szabo, A.; Szymanski, W.; Tai, C.N.P.; Takeuchi, H.; Tanadini-Lang, S.; Tang, F.; Tao, W.; Theron, G.; Tian, C.F.; Tian, Y.-S.; Tuttle, L.M.; Valenti, A.; Verlot, P.; Walker, M.; Wang, J.; Welter, D.; Winslade, M.; Wu, D.; Wu, Y.-R.; Xiao, H.; Xu, B.; Xu, J.; Xu, Z.; Yang, D.; Yang, M.; Yankilevich, P.; You, Y.; Yu, C.; Zhan, J.; Zhang, G.; Zhang, K.; Zhang, T.; Zhang, Y.; Zhao, G.; Zhao, J.; Zhou, X.; Zhu, Z.; Ajani, P.A.; Anazodo, U.C.; Bagloee, S.A.; Bail, K.; Bar, I.; Bathelt, J.; Benkeser, D.; Bernier, M.L.; Blanchard, A.M.; Boakye, D.W.; Bonatsos, V.; Boon, M.H.; Bouboulis, G.; Bromfield, E.; Brown, J.; Bul, K.C.M.; Burton, K.J.; Butkowski, E.G.; Carroll, G.; Chao, F.; Charrier, E.E.; Chen, X.; Chen, Y.-C.; Chenguang; Choi, J.R.; Christoffersen, T.; Comel, J.C.; Cosse, C.; Cui, Y.; van Dessel, P.; Dhaval; Diodato, D.; Duffey, M.; Dutt, A.; Egea, L.G.; El-Said, M.; Faye, M.; Fernandez-Fernandez, B.; Foley, K.G.; Founou, L.L.; Fu, F.; Gadelkareem, R.A.; Galimov, E.; Garip, G.; Gemmill, A.; Gouil, Q.; Grey, J.; Gridneva, Z.; Grothe, M.J.; Gr{\'e}bert, T.; Guerrero, F.; Guignard, L.; Haenssgen, M.J.; Hasler, D.; Holgate, J.Y.; Huang, A.; Hulse-Kemp, A.M.; Jean-Quartier, C.; Jeon, S.-M.; Jia, Y.; Jutzeler, C.; Kalatzis, P.; Karim, M.; Karsay, K.; Keitel, A.; Kempe, A.; Keown, J.R.; Khoo, C.M.; Khwaja, N.; Kievit, R.A.; Kosanic, S.; Koutoukidis, D.A.; Kramer, P.; Kumar, D.; Kira?, N.; Lanza, G.; Le, T.D.; Leem, J.W.; Leightley, D.; Leite, A.; Lercher, L.; Li, Y.; Lim, R.; Lima, L.R.A.; Lin, L.; Ling, T.; Liu, Y.; Liu, Z.; Lu, Y.; Lum, F.M.; Luo, H.; Machhi, J.; Macleod, A.; Macwan, I.; Madala, H.R.; Madani, N.; de Maio, N.; Makowiecki, K.; Mallinson, D.J.; Margelyte, R.; Maria, C.; Markonis, Y.; Marsili, L.; Mavoa, S.; McWilliams, L.; Megersa, M.; Souto-Maior, C.; Menichetti, J.; Mercieca-Bebber, R.; Miller, J.J.; Minde, D.-P.M.; Minges, A.; Mishra, E.; Mishra, V.R.; Moores, C.; Morrice, N.; Moskalensky, A.E.; Navarin, N.; Negera, E.; Nolet, P.; Nordberg, A.; Nord{\'e}n, R.; Nowicki, J.P.; Olova, N.; Olszewski, P.; Onzima, R.; Pan, C.-L.; Park, C.; Park, D.I.; Park, S.; Patil, C.D.; Pedro, S.A.; Perry, S.R.; Peter, J.; Peterson, B.M.; Pezzuolo, A.; d; Pozdnyakov, I.; Qian, S.; Qin, L.; Rafe, A.; Raote, I.; Raza, A.; Rebl, H.; Refai, O.; Regan, T.; Richa, T.; Richardson, M.F.; Robinson, K.R.; Rossoni, L.; Rouet, R.; Safaei, S.; Schneeberger, P.H.H.; Schwotzer, D.; Sebastian, A.; Selinski, J.; Seltmann, S.; Sha, F.; Shalev, N.; Shang, J.-L.; Singer, J.; Singh, M.; Smith, T.; Solomon-Moore, E.; Song, L.; Soraggi, S.; Stanley, R.; Steckhan, N.; Strobl, F.; Subissi, L.; Supriyanto, I.; Surve, C.R.; Suzuki, T.; Syme, C.; S{\"o}relius, K.; Tang, Y.; Tantawy, M.; Tennakoon, S.; Teseo, S.; Toelzer, C.; Tomov, N.; Tovar, M.; Tran, L.; Tripathi, S.; Tuladhar, A.M.; Ukubuiwe, A.C.; Ung, C.O.L.; Valgepea, K.; Vatanparast, H.; Vidal, A.; Wang, F.; Wang, Q.; Watari, R.; Webster, R.; Webster, R.; Wei, J.; Wibowo, D.; Wingenbach, T.S.H.; Xavier, R.M.; Xiao, S.; Xiong, P.; Xu, S.; Xu, S.; Yao, R.; Yao, W.; Yin, Q.; Yu, Y.; Zaitsu, M.; Zeineb, Z.; Zhan, X.-Y.; Zhang, J.; Zhang, R.; Zhang, W.; Zhang, X.; Zheng, S.; Zhou, B.; Zhou, X.; Ahmad, H.; Akinwumi, S.A.; Albery, G.F.; Alhowimel, A.; Ali, J.; Alshehri, M.; Alsuhaibani, M.; Anikin, A.; Azubuike, S.O.; Bach-Mortensen, A.; Baltiansky, L.; Bartas, M.; Belachew, K.Y.; Bhardwaj, V.; Binder, K.; Bland, N.S.; Boah, M.; Bullen, B.; Calabr{\`o}, G.E.; Callahan, T.J.; Cao, B.; Chalmers, K.; Chang, W.; Che, Z.; Chen, A.T.Y.; Chen, H.; Chen, H.; Chen, Y.; Chen, Z.; Choi, Y.; Chowdhury, M.A.K.; Christensen, M.R.; Cooke, R.S.C.; Cottini, M.; Covington, N.V.; Cunningham, C.; Delarocque, J.; Devos, L.; Dhar, A.R.; Ding, K.-F.; Dong, K.; Dong, Z.; Dreyer, N.; Ekstrand, C.; Fardet, T.; Feleke, B.E.; Feurer, T.; Freitas, A.; Gao, T.; Gebremedhin; Giganti, F.; Grabowski, P.; Guerra-Mora, J.R.; Guo, C.; Guo, X.; Gupta, H.; He, S.; Heijne, M.; Heinemann, S.; Hogrebe, A.; Huang, Z.; Iskander-Rizk, S.; Iyer, L.M.; Jahan, Y.; James, A.S.; Joel, E.; Joffroy, B.; J{\'e}gousse, C.; Kambondo, G.; Karnati, P.; Kaya, C.; Ke, A.; Kelly, D.; Kickert, R.; Kidibule, P.E.; Kieselmann, J.P.; Kim, H.J.; Kitazawa, T.; Lamberts, A.; Li, Y.; Liang, H.; Linn, S.N.; Litfin, T.; Liusuo, W.; Lygirou, V.; Mahato, A.K.; Mai, Z.-M.; Major, R.W.; Mali, S.; Mallis, P.; Mao, W.; Marvin-Dowle, K.; Mason, L.D.; Merideth, B.; Merino-Plaza, M.J.; Merlaen, B.; Messina, R.; Mishra, A.K.; Muhammad, J.; Musinguzi, C.; Nanou, A.; Naqash, A.; Nguyen, J.T.; Nguyen, T.T.H.; Ni, D.; Nida; Notcovich, S.; Ohst, B.; Ollivier, Q.R.; Osses, D.F.; Peng, X.; Plantinga, A.; Pulia, M.; Rafiq, M.; Raman, A.; Raucher-Ch{\'e}n{\'e; Rawski, R.; Ray, A.; Razak, L.A.; Rudolf, K.; Rusch, P.; Sadoine, M.L.; Schmidt, A.; Schurr, R.; Searles, S.; Sharma, S.; Sheehan, B.; Shi, C.; Shohayeb, B.; Sommerlad, A.; Strehlow, J.; Sun, X.; Sundar, R.; Taherzadeh, G.; Tahir, N.D.M.; Tang, J.; Testa, J.; Tian, Z.; Tingting, Q.; Verheijen, G.P.; Vickstrom, C.; Wang, T.; Wang, X.; Wang, Z.; Wei, P.; Wilson, A.; d; Wyar; Yassine, A.-A.; Yousefzadeh, A.; Zare, A.; Zeng, Z.; Zhang, C.; Zhang, H.; Zhang, L.; Zhang, T.; Zhang, W.; Zhang, Z.; Zhou, J.; Zhu, D.; Adamo, V.; Adeyemo, A.A.; Aggelidou, M.; Al-Owaifeer, A.M.; Al-Riyami, A.Z.; Alzghari, S.K.; Andersen, V.; Angus, K.; Asaduzzaman, M.; Asady, H.; Ato, D.; Bai, X.; Baines, R.L.; Ballantyne, M.; Ban, B.; Beck, J.; Ben-Nafa, W.; Black, E.; Blancher, A.; Blankstein, R.; Bodagh, N.; Borges, P.; Brooks, A.; Brox-Ponce, J.; Brunetti, A.; Canham, C.D.; Carninci, P.; Carvajal, R.; Chang, S.C.; Chao, J.; Chatterjee, P.; Chen, H.; Chen, L.; Chen, Y.-C.; Chhatriwalla, A.K.; Chikowe, I.; Chuang, T.-J.; Collevatti, R.G.; Cornejo, D.A.V.; Cuenda, A.; Dao, M.; Dauga, D.; Deng, Z.; Devkota, K.; Doan, L.V.; Elewa, Y.H.A.; Fan, D.; Faruk, M.; Feifei, S.; Ferguson, T.S.; Fleres, F.; Foster, E.J.; Foster, S.; Furer, T.; Gao, Y.; Garcia-Rivera, E.J.; Gazdar, A.; George, R.B.; Ghosh, S.; Gianchecchi, E.; Gleason, J.M.; Hackshaw, A.; Hall, A.; Hall, R.; Harper, P.; Hogg, W.E.; Huang, G.; Hunter, K.E.; Ijzerman, A.P.; Jesus, C.; Jian, G.; Lewis, J.S.; Kanj, S.S.; Kaur, H.; Kelly, S.; Kheir, F.; Kichatova, V.S.; Kiyani, M.; Klein, R.; Kovesi, T.; Kraschnewski, J.L.; Kumar, A.P.; Labutin, D.; Lazo-Langner, A.; Leclercq, G.; Li, M.; Li, Q.; Li, T.; Li, Y.; Liao, W.-T.; Liao, Z.-Y.; Lin, J.; Lizer, J.; Lobreglio, G.; Lowies, C.; Lu, C.; Majeed, H.; Martin, A.; Martinez-Sobrido, L.; Meresh, E.; Middelveen, M.; Mohebbi, A.; Mota, J.; Mozaheb, Z.; Muyaya, L.; Nandhakumar, A.; Ng, S.H.X.; Obeidat, M.; Oh, D.-H.; Owais, M.; Pace-Asciak, P.; Panwar, A.; Park, C.; Patterson, C.; Penagos-Tabaree, F.; Pianosi, P.T.; Pinzi, V.; Pridans, C.; Psaroulaki, A.; Pujala, R.K.; Pulido-Arjona, L.; Qi, P.-F.; Rahman, P.; Rai, N.K.; Rassaf, T.; Refardt, J.; Ricciardi, W.; Riess, O.; Rovas, A.; Sacks, F.M.; Saleh, S.; Sampson, C.; Schmutz, A.; Sepanski, R.; Sharma, N.; Singh, M.; Spearman, P.; Subramaniapillai, M.; Swali, R.; Tan, C.M.; Tellechea, J.I.; Thomas, L.-M.; Tong, X.; Vavvas, D.G.; Veys, R.; Vitriol, V.; Wang, H.-D.; Wang, J.; Wang, J.; Waugh, J.; Webb, S.A.; Williams, B.A.; Workman, A.D.; Xiang, T.; Xie, L.-X.; Xu, J.; Xu, T.; Yang, C.; Yoon, J.G.; Yuan, C.M.; Zaritsky, A.; Zhang, Y.; Zhao, H.; Zuckerman, H.; Lyu, R.; Pullan, W.
  Database, 2019, 2019年
  研究論文（学術雑誌）
• The efficacy of chronic zinc oxide nanoparticles using on testicular damage in the streptozotocin-induced diabetic rat model
  Eman I. El-behery; Nesma I. El-naseery; Hanaa M. El-Ghazali; Yaser H.A. Elewa; Eman A.A. Mahdy; Enas El-Hady; Mervat M.H. Konsowa
  Acta Histochemica, 121, 1, 84, 93, Elsevier GmbH, 2019年01月01日
  英語, 研究論文（学術雑誌）
• Slc:Hartley guinea pigs frequently possess duplication of the caudal vena cava
  Teppei NAKAMURA; Miyuki NORIMURA; Kanako SUMI; Osamu ICHII; Yaser Hosny Ali ELEWA; Yasuhiro KON; Osamu TATSUMI; Hideki HATTORI; Tomoji YOSHIYASU; Ken-ichi NAGASAKI
  Experimental Animals, 68, 4, 465, 470, 2019年, ［査読有り］
  英語, 研究論文（学術雑誌）
• Corrigendum: Urinary exosome-derived microRNAs reflecting the changes in renal function in cats [Front. Vet. Sci., 5, (2018), (289)] doi: 10.3389/fvets.2018.00289
  Ichii, O.; Ohta, H.; Horino, T.; Nakamura, T.; Hosotani, M.; Mizoguchi, T.; Morishita, K.; Nakamura, K.; Sasaki, N.; Takiguchi, M.; Sato, R.; Oyamada, K.; Elewa, Y.H.A.; Kon, Y.
  Frontiers in Veterinary Science, 6, JAN, 2, 2019年, ［査読有り］
  研究論文（学術雑誌）
• Overexpression of toll-like receptor 9 correlates with podocyte injury in a murine model of autoimmune membranoproliferative glomerulonephritis
  Md. Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Teppei Nakamura; Yuki Otani; Marina Hosotani; Yasuhiro Kon
  Autoimmunity, 1, 13, Informa {UK} Limited, 2018年12月, ［査読有り］
  研究論文（学術雑誌）
• Morphofunctional analysis of antigen uptake mechanisms following sublingual immunotherapy with beads in mice
  Yaser Hosny Ali Elewa; Tatsuya Mizoguchi; Osamu Ichii; Teppei Nakamura; Yasuhiro Kon
  PLOS ONE, 13, 12, e0201330, Public Library of Science ({PLoS}), 2018年12月, ［査読有り］
  研究論文（学術雑誌）
• An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model.
  Nesma Ibraheim El-Naseery; Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 220, 9, 20, 2018年11月, ［国際誌］
  英語, 研究論文（学術雑誌）, The current study was conducted on a menopause rat model induced by ovariectomy to assess the histological and immunohistochemical alterations in the parotid glands and to verify the efficiency of human umbilical cord derived-mesenchymal stromal cell (hUCB-MSCs) in treating this condition. Eighteen adult female rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX) and OVX injected with hUCB-MSCs (OVX+hUCB-MSCs). At 3months post-ovariectomy, the salivary flow rate and size of the parotid glands were measured. The parotid glands were histologically investigated via H&E stained sections. Furthermore, immunohistochemical analysis for human CD105, human CD34, proliferating cell nuclear antigen (PCNA), single strand DNA (ssDNA), caspase 3, aquaporin (AQP)1, α-smooth muscle actin (α-SMA) and mouse CD34 were performed. The OVX group showed interstitial hemorrhage, dispersed acini and intracytoplasmic vacuoles in the acinar cells. Furthermore, immunohistochemical staining revealed a significant decrement in the number of ssDNA positive apoptotic cells, but a significant increment of PCNA positive proliferating cells, AQP1 positive blood capillaries, α-SMA positive myoepithelial cells and endogenous CD34 positive hematopoietic progenitor cells in the OVX+hUCB-MSCs group as compared with the OVX group. These findings suggest a potential regenerative therapy of MSCs to injured parotid gland structures. However, further investigations are required to illustrate the mechanism of hUCB-MSCs mediated parotid gland regeneration.

  
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• Restricted localization of ultimobranchial body remnants and parafollicular cells in the one-humped camel (Camelus dromedarius).
  Teppei Nakamura; Yaser Hosny Ali Elewa; Osamu Ichii; Marina Hosotani; Wael A M Ghonimi; Osamu Tatsumi; Ken-Ichi Nagasaki; Yasuhiro Kon
  The Journal of veterinary medical science, 80, 9, 1368, 1372, 2018年09月13日, ［国内誌］
  英語, 研究論文（学術雑誌）

  
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• Abnormal Morphology of Distal Tubular Epithelial Cells Is Regulated by Genetic Factors Derived from Mouse Chromosome 12.
  Osamu Ichii; Teppei Nakamura; Taro Horino; Akira Yabuki; Yaser H A Elewa; Yasuhiro Kon
  The American journal of pathology, 188, 9, 2120, 2138, 2018年09月, ［国際誌］
  英語, 研究論文（学術雑誌）, The distal tubule (DT) helps regulate blood pressure and electrolytes. We describe a novel, autosomal recessive, morphofunctional DT abnormality in inbred mice evident as columnar alternations and age-related cystic changes. This abnormality developed in both sexes of DBA/2Cr. Similar phenotypes were observed in A/J, C3H/He, DBA/1J, and FVB/N strains, but not in AKR/N, BALB/c, or C57BL/6N strains. In DBA/2Cr, abnormal DT localized to straight and convoluted segments and showed IL-36α DT injury marker expression. However, DT epithelial proliferation, examined by bromodeoxyuridine incorporation, was not remarkably altered with the progression of abnormality. Abnormal DT epithelial cells in DBA/2Cr displayed elongated primary cilia, loose intercellular adhesions, and numerous vesicles with altered localization of CD9, Na+/K+ATPase, and E-cadherin, indicating altered cell function, adhesion, and polarity. DBA/2Cr-type D12Mit182-D12Mit83 was identified as a candidate locus designated DBA/2 renal cyst (drecy). Within drecy, the gene regulated by estrogen in breast cancer protein (Greb1) transcript variant 2 was significantly up-regulated in DBA/2Cr kidney versus C57BL/6N. Greb1 localized to DT cytoplasm in C57BL/6 and to cytoplasm and nucleus in DBA/2Cr. Greb1-overexpressing M-1 kidney cells showed an altered epithelial-mesenchyme phenotype. B6.D2-(D12Mit182-D12Mit83) congenic mice carrying drecy did not show DT abnormalities, whereas DBA/2Cr × B6.D2-(D12Mit182-D12Mit83) mice did. Identification of this novel DT abnormality regulated by a DBA/2Cr mouse chromosome 12-derived locus and additional genetic factors improve the understanding of DT pathogenesis.

  
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• Spirulina platensis attenuates the associated neurobehavioral and inflammatory response impairments in rats exposed to lead acetate
  Samah R. Khalil; Hesham A. Khalifa; Sabry M. Abdel-Motal; Hesham H. Mohammed; Yaser H.A. Elewa; Hend Atta Mahmoud
  Ecotoxicology and Environmental Safety, 157, 255, 265, Academic Press, 2018年08月15日
  英語, 研究論文（学術雑誌）

  
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• Cotton rat (Sigmodon hispidus) develops metabolic disorders associated with visceral adipose inflammation and fatty pancreas without obesity.
  Nakamura T; Ichii O; Irie T; Kouguchi H; Sotozaki K; Chihara M; Sunden Y; Nagasaki KI; Tatsumi O; Elewa YHA; Kon Y
  Cell Tissue Res, 375, 2, 483, 492, 2018年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Protective effects of miswak (salvadora persica) against experimentally induced gastric ulcers in rats
  Lebda, M.A.; El-Far, A.H.; Noreldin, A.E.; Elewa, Y.H.A.; Al Jaouni, S.K.; Mousa, S.A.
  Oxidative Medicine and Cellular Longevity, 2018, 6703296, 2018年07月09日
  英語, 研究論文（学術雑誌）
• Modified scanning electron microscopy reveals pathological crosstalk between endothelial cells and podocytes in a murine model of membranoproliferative glomerulonephritis.
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Teppei Nakamura; Yuki Otani; Marina Hosotani; Yasuhiro Kon
  Scientific reports, 8, 1, 10276, 10276, 2018年07月06日, ［国際誌］
  英語, 研究論文（学術雑誌）, This study evaluated endothelial cells and podocytes, both being primary components of the glomerular filtration barrier, in the progression of membranoproliferative glomerulonephritis (MPGN) using modified scanning electron microscopy (mSEM) analysis. BXSB/MpJ-Yaa model mice exhibited autoimmune-mediated MPGN characterised by elevated serum autoantibody levels, albuminuria, renal dysfunctional parameters, and decreased glomerular endothelial fenestrations (EF) and podocyte foot process (PFP) effacement with immune cell infiltration. Similar to transmission electron microscopy, mSEM revealed a series of pathological changes in basement membrane and densities of EF and PFP in BXSB/MpJ-Yaa compared with control BXSB/MpJ at different stages. Further, immunopositive area of endothelial marker (CD34), podocyte functional molecules (Nephrin, Podocin, Synaptopodin, and Wilms' tumour 1 (WT1)), and vascular endothelial growth factor A (VEGF A) significantly decreased in the glomerulus of BXSB/MpJ-Yaa compared with BXSB at final stage. The indices of glomerular endothelial injuries (EF density and immunopositive area of CD34 and VEGF A) and podocyte injuries (PEP density and immunopositive area of podocyte functional molecules) were also significantly correlated with each other and with indices of autoimmune disease and renal dysfunction. Thus, our results elucidated the pathological crosstalk between endothelial cells and podocytes in MPGN progression and the usefulness of mSEM for glomerular pathological analysis.

  
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• Cotton rats (Sigmodon hispidus) possess pharyngeal pouch remnants originating from different primordia
  Teppei Nakamura; Osamu Ichii; Takao Irie; Tatsuya Mizoguchi; Akio Shinohara; Hirokazu Kouguchi; Yuji Sunden; Saori Otsuka-Kanazawa; Yaser Hosny Ali Elewa; Chihiro Koshimoto; Ken-Ichi Nagasaki; Yasuhiro Kon
  Histology and Histopathology, 33, 6, 555, 565, 2018年06月01日
  英語, 研究論文（学術雑誌）
• Immunohistochemical localization of osteoblast activating peptide in the mouse kidney
  Ahmed E. Noreldin; Yaser Hosny Ali Elewa; Yasuhiro Kon; Katsuhiko Warita; Yoshinao Z. Hosaka
  Acta Histochemica, 120, 4, 323, 328, 2018年05月01日
  英語, 研究論文（学術雑誌）

  
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• Close pathological correlations between chronic kidney disease and reproductive organ-associated abnormalities in female cotton rats.
  Osamu Ichii; Teppei Nakamura; Takao Irie; Hirokazu Kouguchi; Kozue Sotozaki; Taro Horino; Yuji Sunden; Yaser Hosny Ali Elewa; Yasuhiro Kon
  Experimental biology and medicine (Maywood, N.J.), 243, 5, 418, 427, 2018年03月, ［国際誌］
  英語, 研究論文（学術雑誌）, Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious diseases. We previously clarified that cotton rats, particularly females, developed chronic kidney disease characterized by cystic lesions, inflammation, and fibrosis. The present study investigated female-associated factors for chronic kidney disease development in cotton rats. Notably, female cotton rats developed separation of the pelvic symphysis and hypertrophy in the vaginal parts of the cervix with age, which strongly associated with pyometra. The development of pyometra closely associated with the deterioration of renal dysfunction or immunological abnormalities was indicated by blood urea nitrogen and serum creatinine or spleen weight and serum albumin/globulin ratio, respectively. These parameters for renal dysfunction and immunological abnormalities were statistically correlated. These phenotypes found in the female reproductive organs were completely inhibited by ovariectomy. Further, the female cotton rats with pyometra tended to show more severe chronic kidney disease phenotypes and immunological abnormalities than those without pyometra; these changes were inhibited in ovariectomized cotton rats. With regard to renal histopathology, cystic lesions, inflammation, and fibrosis were ameliorated by ovariectomy. Notably, the immunostaining intensity of estrogen receptor α and estrogen receptor β were weak in the healthy kidneys, but both estrogen receptors were strongly induced in the renal tubules showing cystic changes. In conclusion, the close correlations among female reproductive organ-associated abnormalities, immunological abnormalities, and renal dysfunction characterize the chronic kidney disease features of female cotton rats. Thus, the cotton rat is a unique rodent model to elucidate the pathological crosstalk between chronic kidney disease and sex-related factors. Impact statement The increasing number of elderly individuals in the overall population has led to a concomitant age-related increase in chronic kidney disease. Moreover, the global prevalence of patients with chronic kidney disease is gradually increasing, which poses a serious public health problem. The limited number of spontaneous chronic kidney disease animal models, which resemble chronic kidney disease pathogenesis in elderly individuals, is a major limitation in the development of experimental and curative medicines for chronic kidney disease. This pathological study clarified that sex-related factors, including hormones, and abnormalities of the female reproductive system, such as pyometra, are closely associated with chronic kidney disease development by using cotton rats ( Sigmodon hispidus). Further, ovariectomy inhibited the phenotypes of the female reproductive system, immunological abnormalities, and chronic kidney disease. Thus, this laboratory rodent serves as a novel and useful spontaneous chronic kidney disease model to elucidate the candidate disease factors and the pathogenesis of chronic kidney disease both in human and experimental medicine.

  
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• Histopathological correlations between mediastinal fat-associated lymphoid clusters and the development of lung inflammation and fibrosis following bleomycin administration in mice
  Yaser Hosny Ali Elewa; Osamu Ichii; Kensuke Takada; Teppei Nakamura; Md. Abdul Masum; Yasuhiro Kon
  Frontiers in Immunology, 9, 271, Frontiers Media S.A., 2018年02月15日
  英語, 研究論文（学術雑誌）

  
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• Ovarian mast cells migrate toward ovary-fimbria connection in neonatal MRL/MpJ mice.
  Teppei Nakamura; Masataka Chihara; Osamu Ichii; Saori Otsuka-Kanazawa; Ken-Ichi Nagasaki; Yaser Hosny Ali Elewa; Osamu Tatsumi; Yasuhiro Kon
  PloS one, 13, 4, e0196364, 2018年, ［国際誌］
  英語, 研究論文（学術雑誌）
• Possible role of Arthrospira platensis in reversing oxidative stress-mediated liver damage in rats exposed to lead
  Samah R. Khalil; Walaa M. Elhady; Yaser H.A. Elewa; Noura E. Abd El-Hameed; Sozan A. Ali
  Biomedicine and Pharmacotherapy, 97, 1259, 1268, Elsevier Masson SAS, 2018年01月01日
  英語, 研究論文（学術雑誌）

  
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• Pigeon and fowl hearts: A comparative morphological and immunohistochemical (Troponin T) study
  Yaser H.A. Elewa; Sherif Kh.A. Mohamed; Attia A.A. Moselhy; Dina M.M. AlSadek; Haitham A. Badr
  Slovenian Veterinary Research, 55, 393, 403, University of Ljubljana - Veterinary Faculty, 2018年
  英語, 研究論文（学術雑誌）
• Urinary exosome-derived microRNAs reflecting the changes in renal function in cats
  Ichii, O.; Ohta, H.; Horino, T.; Nakamura, T.; Hosotani, M.; Mizoguchi, T.; Morishita, K.; Nakamura, K.; Sasaki, N.; Takiguchi, M.; Sato, R.; Oyamada, K.; Elewa, Y.H.A.; Yasuhiro, K.
  Frontiers in Veterinary Science, 5, NOV, 289, 2018年, ［査読有り］
  研究論文（学術雑誌）
• Autoimmune abnormality affects ovulation and oocyte-pick-up in MRL/MpJ-Fas(lpr/lpr) mice
  M. Hosotani; O. Ichii; T. Nakamura; S. O. Kanazawa; Y. Hosny Ali Elewa; Y. Kon
  LUPUS, 27, 1, 82, 94, 2018年01月, ［査読有り］
  英語, 研究論文（学術雑誌）
• IL-36α Regulates Tubulointerstitial Inflammation in the Mouse Kidney.
  Ichii O; Kimura J; Okamura T; Horino T; Nakamura T; Sasaki H; Elewa YHA; Kon Y
  Frontiers in Immunology, 8, 1346, 2017年10月
  英語, 研究論文（学術雑誌）

  
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• Local CD34-positive capillaries decrease in mouse models of kidney disease associating with the severity of glomerular and tubulointerstitial lesions
  Md Abdul Masum; Osamu Ichii; Yaser Hosny Ali Elewa; Teppei Nakamura; Yasuhiro Kon
  BMC NEPHROLOGY, 18, 280, 2017年09月
  英語, 研究論文（学術雑誌）

  
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• Effects of a mixture of medetomidine, midazolam and butorphanol on anesthesia and blood biochemistry and the antagonizing action of atipamezole in hamsters
  Teppei Nakamura; Naoya Karakida; Ai Dantsuka; Osamu Ichii; Yaser Hosny Ali Elewa; Yasuhiro Kon; Ken-ichi Nagasaki; Hideki Hattori; Tomoji Yoshiyasu
  JOURNAL OF VETERINARY MEDICAL SCIENCE, 79, 7, 1230, 1235, 2017年07月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11
  Osamu Ichii; Masataka Chihara; Shin-Hyo Lee; Teppei Nakamura; Saori Otsuka-Kanazawa; Taro Horino; Yaser Hosny Ali Elewa; Yasuhiro Kon
  AUTOIMMUNITY, 50, 2, 114, 124, 2017年03月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Sex-related differences in autoimmune-induced lung lesions in MRL/MpJ-fas(lpr) mice are mediated by the development of mediastinal fat-associated lymphoid clusters
  Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  AUTOIMMUNITY, 50, 5, 306, 316, 2017年, ［査読有り］
  英語, 研究論文（学術雑誌）
• Degenerative and regenerative features of myofibers differ among skeletal muscles in a murine model of muscular dystrophy
  Teppei Ikeda; Osamu Ichii; Saori Otsuka-Kanazawa; Teppei Nakamura; Yaser Hosny Ali Elewa; Yasuhiro Kon
  JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 37, 4-5, 153, 164, 2016年10月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Rapid specimen preparation to improve the throughput of electron microscopic volume imaging for three-dimensional analyses of subcellular ultrastructures with serial block-face scanning electron microscopy
  Truc Quynh Thai; Huy Bang Nguyen; Sei Saitoh; Bao Wu; Yurika Saitoh; Satoshi Shimo; Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon; Takashi Takaki; Kensuke Joh; Nobuhiko Ohno
  MEDICAL MOLECULAR MORPHOLOGY, 49, 3, 154, 162, 2016年09月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes
  Osamu Ichii; Teppei Nakamura; Takao Irie; Hirokazu Kouguchi; Daisuke Nakamura; Saori Nakamura; Shinobu Sato; Keisuke Yokoyama; Taro Horino; Yuji Sunden; Yaser Hosny Ali Elewa; Yasuhiro Kon
  HISTOCHEMISTRY AND CELL BIOLOGY, 146, 3, 351, 362, 2016年09月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Spatiotemporal distribution of extracellular matrix changes during mouse duodenojejunal flexure formation
  Sawa Onouchi; Osamu Ichii; Teppei Nakamura; Yaser Hosny Ali Elewa; Yasuhiro Kon
  CELL AND TISSUE RESEARCH, 365, 2, 367, 379, 2016年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• MRL/MpJ mice show unique pathological features after experimental kidney injury
  Daichi Shiozuru; Osamu Ichii; Junpei Kimura; Teppei Nakamura; Yaser Hosny Ali Elewa; Saori Otsuka-Kanazawa; Yasuhiro Kon
  HISTOLOGY AND HISTOPATHOLOGY, 31, 2, 189, 204, 2016年02月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Comparative analysis of mediastinal fat-associated lymphoid cluster development and lung cellular infiltration in murine autoimmune disease models and the corresponding normal control strains
  Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon
  IMMUNOLOGY, 147, 1, 30, 40, 2016年01月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Genetic factors derived from the MRL/MpJ mouse function to maintain the integrity of spermatogenesis after heat exposure
  M. Chihara; T. Nakamura; S. Otsuka-Kanazawa; O. Ichii; Y. H. A. Elewa; Y. Kon
  ANDROLOGY, 3, 5, 991, 999, 2015年09月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Comparative protective effects of royal jelly and cod liver oil against neurotoxic impact of tartrazine on male rat pups brain
  Amany Abdel-Rahman Mohamed; Azza A. A. Galal; Yaser H. A. Elewa
  ACTA HISTOCHEMICA, 117, 7, 649, 658, 2015年, ［査読有り］
  英語, 研究論文（学術雑誌）
• Characterization of mouse mediastinal fat-associated lymphoid clusters
  Yaser Hosny Ali Elewa; Osamu Ichii; Saori Otsuka; Yoshiharu Hashimoto; Yasuhiro Kon
  CELL AND TISSUE RESEARCH, 357, 3, 731, 741, 2014年09月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Structural Changes of Goat Parotid Salivary Gland: Pre- and Post-Weaning Periods
  Y. H. A. Elewa; O. Ichii; S. Otsuka; Y. Hashimoto; Y. Kon
  ANATOMIA HISTOLOGIA EMBRYOLOGIA, 43, 4, 265, 272, 2014年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Antidotal impact of extra virgin olive oil against genotoxicity, cytotoxicity and immunotoxicity induced by hexavalent chromium in rat
  Khalil, S.; Awad, A.; Elewa, Y.
  International Journal of Veterinary Science and Medicine, 1, 2, 2013年
  研究論文（学術雑誌）
• Ovarian cysts in MRL / MpJ mice are derived from the extraovarian rete: a developmental study.
  Lee SH; Ichii O; Otsuka S; Elewa YH; Namiki Y; Hashimoto Y; Kon Y
  Journal of anatomy, 219, 6, 743, 755, 2011年12月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Erratum: Cytoarchitectural differences of myoepithelial cells among goat major salivary glands (Veterinary Research Communications DOI: 10.1007/s11259-010-9427-9)
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abuel-Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shin-Hyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon
  Veterinary Research Communications, 34, 8, 727, 2010年12月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Cytoarchitectural differences of myoepithelial cells among goat major salivary glands
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abu Al Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shin-Hyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon
  VETERINARY RESEARCH COMMUNICATIONS, 34, 6, 557, 567, 2010年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Structural characteristics of goat (Capra hircus) parotid salivary glands
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abuel-Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shinhyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon
  JAPANESE JOURNAL OF VETERINARY RESEARCH, 58, 2, 121, 135, 2010年08月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Dietary supplementation with arachidonic acid but not eicosapentaenoic or docosahexaenoic acids alter lipids metabolism in C57BL/6J mice
  Sameh Magdeldin; Yaser Elewa; Takako Ikeda; Junko Ikei; Ying Zhang; Bo Xu; Masaaki Nameta; Hidehiko Fujinaka; Yutaka Yoshida; Eishin Yaoita; Tadashi Yamamoto
  GENERAL PHYSIOLOGY AND BIOPHYSICS, 28, 3, 266, 275, 2009年09月
  英語, 研究論文（学術雑誌）

  
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• MFALCs reprogramming: Innovative therapeutic strategy of lung cancer following Controlled immunotherapy Delivery.”
  Y. H. A. Elewa, The AAPS-ESPS congress “Towards a New Horizon of Physiology in Africa”. Mercury Hotel, Ismailia, Egypt. Keynote speaker. “, 2025年02月
• Novel mediastinal immune clusters driving lung injury pathogenesis following inflammation or diabetic induction.”
  Y. H. A. Elewa, The 44th Annual Conference of Ain Shams University Medical Congress. Triumph Katameya Hotel, New Cairo, Egypt., 2024年10月
• Immunostimulant impact of intravenous/intranasal polyinosinic–polycytidylic acid (Poly I:C) administration on the mediastinal fat-associated lymphoid clusters and lung tissue in healthy mice
  Y. H. A. Elewa, The 11th Sapporo Summer Symposium for One Health (SaSSOH 2023) Hokkaido University, Sapporo, Hokkaido, Japan., 2023年09月
  英語, その他
• Novel Fat Associated Lymphoid clusters with special focus on the CHICKEN AIR SACS AND MESENTERY: A HISTOMORPHOMETRICAL AND IMMUNOLOGICAL STUDY
  Y. H. A. Elewa; Md Rashedul Islam; Sherif Kh; A. Mohamed, The 17th College Scientific Conference of Faculty of Veterinary medicine, Zagazig University “Veterinary Medicine and One Health Concept”. October 12th-15th, 2022, Hawaii Paradise, Hurghada, Egypt., 2022年10月
• Mediastinal fat associated lymphoid clusters: A novel immune structure controlling respiratory diseases intrathoracic immune hemostasis
  Y. H. A. Elewa; O. Ichii; Y. Kon, The 5th international Conference of Medical Analysis, Toxins, and IVF”. February 11th, 2022; Damanhur; Behera, Egypt. Keynote speaker., 2022年02月
• ヤギとブタの膣前庭で見出される生殖器関連リンパ組織【JST・京大機械翻訳】
  TSOLMON Chuluunbaatar; TSOLMON Chuluunbaatar; 市居修; 市居修; 難波貴志; ABDUL Masum Md.; ALI ELEWA Yaser Hosny; ALI ELEWA Yaser Hosny; 昆泰寛, 日本獣医学会学術集会講演要旨集, 165th (CD-ROM), 2022年
• マウス肛門直腸粘膜で見いだされたリンパ組織とその形態に影響する全身因子【JST・京大機械翻訳】
  RUBEL Md Zahir Uddin; RUBEL Md Zahir Uddin; 市居修; 市居修; 難波貴志; MASUM Md. Abdul; Chuluunbaatar Tsolmon; Chuluunbaatar Tsolmon; ALI ELEWA Yaser Hosny; ALI ELEWA Yaser Hosny; 昆泰寛, 日本獣医学会学術集会講演要旨集, 165th (CD-ROM), 2022年
• マウス全身免疫状態の違いによる異物応答の修飾
  山川 智大; 市居 修; 難波 貴志; 大谷 祐紀; 中村 鉄平; Abdul Masum Md.; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 8], 2021年09月
  (公社)日本獣医学会, 日本語
• 慢性腎炎における尿と腎盤上皮の病態連関
  市居 修; 細谷 実里奈; Masum Md.Abdul; 堀野 太郎; 中村 鉄平; 難波 貴志; 大谷 祐紀; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 26], 2021年09月
  (公社)日本獣医学会, 日本語
• ウシの膣前庭に見いだされた生殖器リンパ輪【JST・京大機械翻訳】
  TSOLMON Chuluunbaatar; TSOLMON Chuluunbaatar; 市居修; ABDUL Masum Md.; ABDUL Masum Md.; HOSNY Ali Elewa Yaser; HOSNY Ali Elewa Yaser; 昆泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 38], 2021年09月
  (公社)日本獣医学会, 英語
• ヤギ腎臓における脈管構造関連リンパ組織の解明【JST・京大機械翻訳】
  MASUM Md Abdul; MASUM Md Abdul; 市居修; 市居修; ALI ELEWA Yaser Hosny; ALI ELEWA Yaser Hosny; 昆泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 25], 2021年09月
  (公社)日本獣医学会, 英語
• Interleukin-36受容体を欠損した自己免疫性腎炎モデルマウスの腎表現型解析
  難波 貴志; 市居 修; 岡村 匡史; 中野 堅太; 中村 鉄平; 大谷 祐紀; Abdul Masum Md.; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 164回, [AO, 27], 2021年09月
  (公社)日本獣医学会, 日本語
• ネコ尿管の形態と尿管結石症による変化
  市居 修; 小山田 和央; 水川 葉月; 中村 鉄平; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 180, 180, 2020年10月
  (公社)日本獣医学会, 日本語
• 自己免疫疾患モデルマウスを用いた糖尿病性腎臓病の病態解明アプローチ
  平松 栞; 市居 修; 難波 貴志; 大谷 祐紀; Masum Md. Abdul; 中村 鉄平; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 181, 181, 2020年10月
  (公社)日本獣医学会, 日本語
• 自己免疫疾患モデルマウスの解析からみる腎臓内IL-36群の機能的棲み分け
  難波 貴志; 市居 修; 中村 鉄平; 大谷 祐紀; Masum Md. Abdul; Yaser Elewa Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 180, 180, 2020年10月
  (公社)日本獣医学会, 日本語
• コットンラットの尾の組織学的特徴および自切機序の解析
  中村 鉄平; 細谷 実里奈; 市居 修; 入江 隆夫; 寸田 祐嗣; Elewa Yaser Hosny Ali; 三嶋 隆; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 163回, 177, 177, 2020年10月
  (公社)日本獣医学会, 日本語
• マウスの実験的閉塞性腎症におけるToll様受容体8シグナル伝達を介した有足細胞傷害【JST・京大機械翻訳】
  MASUM Md. Abdul; MASUM Md. Abdul; 市居修; ELEWA Yaser Hosny Ali; ELEWA Yaser Hosny Ali; 昆泰寛, 日本獣医学会学術集会講演要旨集, 163回, 181, 181, 2020年10月
  (公社)日本獣医学会, 英語
• Uncaria tomentosa (Willd. ex Schult.) DC.: A review on chemical constituents and biological activities
  Gaber El Saber Batiha; Amany Magdy Beshbishy; Lamiaa Wasef; Yaser H.A. Elewa; Mohamed E.Abd El-Hack; Ayman E. Taha; Adham Abdullah Al-Sagheer; Hari Prasad Devkota; Vincenzo Tufarelli, Applied Sciences (Switzerland), 10, 8, 2020年04月
  書評論文,書評,文献紹介等
• Possible role of the novel mediastinal fat associated lymphoid clusters in human, animals and poultry respiratory diseases progression
  Y. H. A. Elewa; O. Ichii, S; T. Nakamura; Y. Kon, The 4th international scientific Veterinary Medicine Conference” Veterinary Medicine: locomotive for development”. October 10-13, 2019, Hurghada, Egypt. Keynote speaker., 2019年10月
• Elucidation of the pathogenesis of lung asthma development and recovery: Possible immune cells’ crosstalk within lung and mediastinal fat associated lymphoid clusters in papain induced lung asthma mice model
  Y. H. A. Elewa; O. Ichii, S; T. Nakamura; Y. Kon. T, The 7th Congress of Asian Association of Veterinary Anatomists (Asian AVA). September 26-27, 2019, Jeju, Korea. Keynote speaker., 2019年09月
• コットンラット(Sigmodon hispidus)卵巣の独特な形態学的特徴(Unique morphological characteristics in the ovary of cotton rat(Sigmodon hispidus))
  Islam Md Rashedul; 市居 修; 中村 鉄平; 入江 隆夫; Masum Md Abdul; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 341, 341, 2019年08月
  (公社)日本獣医学会, 英語
• 自己免疫疾患モデルマウスにみられる涙液産生組織の形態学的変化
  平石 真也; 市居 修; 中村 鉄平; 難波 貴志; Hosny Ali Elewa Yaser; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 162回, 336, 336, 2019年08月
  (公社)日本獣医学会, 日本語
• 新生子MRL/MpJマウスにおいて卵巣マスト細胞は卵巣-卵巣采結合部の方向に遊走する : 局在変化が明らかにするマスト細胞の新たな役割 (特集 マスト細胞研究の新展開)—Ovarian mast cells migrate toward the ovary-fimbria connection in neonatal MRL/MpJ mice : a novel role of mast cells unveiled by their localization changes
  中村 鉄平; 市居 修; Yaser Hosny Ali Elewa; 昆 泰寛, 臨床免疫・アレルギー科 = Clinical immunology & allergology / 臨床免疫・アレルギー科編集委員会 編, 71, 6, 603, 607, 2019年06月
  科学評論社, 日本語
• マウス生体内異物反応の経時観察-組織局所の炎症と線維化の関連性-
  大江紗央; 市居修; 難波貴志; 大谷祐紀; MD. ABDUL Masum; 中村鉄平; 中村鉄平; YASER HOSNY Ali Elewa; 西邑隆徳; 中山泰秀; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019年
• 加齢マウスの尿生殖器における局所リンパ組織の探索
  山下渚; 市居修; 大谷裕紀; MD. ABDUL Masum; YASER HOSNY Ali Elewa; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019年
• 動物の尿路関連リンパ組織(UTALT)に発現する分子群
  市居修; 中村鉄平; 中村鉄平; 堀野太郎; 細谷実里奈; YASER HOSNY Ali Elewa; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019年
• コットンラットの下咽頭梨状窩瘻を構成する細胞の組織化学的特徴
  中村鉄平; 中村鉄平; 市居修; 入江隆夫; 寸田祐嗣; 細谷実里奈; 長崎健一; YASER HOSNY Ali Elewa; 三嶋隆; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019年
• 自己免疫疾患モデルマウスで変化する腎臓内Interleukin 1 familyの発現バランス
  難波貴志; 市居修; 中村鉄平; 中村鉄平; YASER HOSNY Ali Elewa; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019年
• マウスの卵巣嚢-腹腔連絡路(卵巣嚢孔)
  細谷実里奈; 市居修; 中村鉄平; 中村鉄平; 難波貴志; YASER HOSNY Ali Elewa; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019年
• 精巣摘出が自己免疫疾患モデルマウスの病態形成へ与える影響
  大谷祐紀; 市居修; YASER HOSNY Ali Elewa; 中村鉄平; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019年
• “Lymphoid tissues appearing in the urinary tract of animals (Urinary tract-associated lymphoid tissue, UTALT)”.
  O. Ichii; T. Nakamura; R. Ono; T. Horino; Y. H. A. Elewa; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, 2018年09月
  日本語
• “Morphological characteristics of the dromedary thyroid gland - and distribution of C cells”.
  T. Nakamura; O. Ichii; Y. H. A. Elewa; W. A. M. Ghonimi; K. Nagasaki; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, 2018年09月
  日本語
• “New findings of kidney disease - Kidney lesions found in Casp3 deficient mice”.
  T. Suzuki; O. Ichii; T. Nakamura; Y. H. A. Elewa; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, 2018年09月
  日本語
• “Morphological and functional changes observed in bone of autoimmune disease model”.
  T. Namba; O. Ichii; Y. H. A. Elewa; T. Nakamura; J. Kimura; Y. Otani; Md. A. Masum; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, 2018年09月
  日本語
• Investigation for meiotic apoptosis of spermatocyte in autoimmune disease model mouse.
  エレワ ヤセル, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan., 161th, 2018年09月
  日本語
• Effect of systemic autoimmune abnormality in MRL/MpJ-Faslpr/lpr mice on the ciliary function.
  M. Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 161th Meeting of the Japanese Society of Veterinary Sciences. Ibaraki, Tsukuba-shi, Japan, 161th, 2018年09月
  日本語
• “Mediastinal fat-associated lymphoid cluster and lung asthma development in papain induced lung asthma mice model
  Y. H. A. Elewa; T. Nakamura; O. Ichii; Y. Kon, The 64th annual meeting of the Japanese the Anatomical Society (Oral Presentation). Aomori, Japan., 64th, 2018年09月
  英語
• “Overexpression of Toll-like receptor 9 correlates with podocyte injury found in murine autoimmune glomerulonephritis.
  エレワ ヤセル, The 6th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2018. Sapporo, Japan., 161回, 294, 294, 2018年08月
  (公社)日本獣医学会, 英語
• “Glomerular lesions seen in male cotton rats (Sigmodon hispidus)”
  T. Nakamura; O. Ichii; T. Irie; H. Kouguchi; Sunden, Masum; M. A., Y; Y. H. A. Elewa; Y. Kon, The 11th Annual Meeting of the Japanese Society of Veterinary Renal Urology., 11th, 2018年08月
  日本語
• 自己免疫疾患モデルマウスの骨に観察される形態機能的変化
  難波 貴志; 市居 修; 中村 鉄平; 木村 純平; 大谷 祐紀; Masum Md. Abdul; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 285, 285, 2018年08月
  (公社)日本獣医学会, 日本語
• ウシの眼関連リンパ組織について
  小千田 圭吾; 市居 修; 中村 鉄平; 池田 哲平; 山下 祐輔; 長澤 裕哉; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 288, 288, 2018年08月
  (公社)日本獣医学会, 日本語
• MRL/MpJ-Fas lpr/lprマウスの全身自己免疫異常が線毛運動機能に与える影響
  細谷 実里奈; 市居 修; Elewa Yaser Hosny Ali; 中村 鉄平; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 296, 296, 2018年08月
  (公社)日本獣医学会, 日本語
• 腎疾患の新知見 Casp3欠損マウスにみられる腎病変
  鈴木 崇史; 市居 修; 中村 鉄平; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 294, 294, 2018年08月
  (公社)日本獣医学会, 日本語
• 自己免疫疾患モデルマウスで増加する減数分裂特異的精母細胞アポトーシスの原因探索
  大谷 祐紀; 市居 修; Elewa Yaser Hosny Ali; 木村 純平; Masum Md. Abdul; 中村 鉄平; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 295, 295, 2018年08月
  (公社)日本獣医学会, 日本語
• 動物の尿路に出現するリンパ組織(Urinary tract-associated lymphoid tissue, UTALT)
  市居 修; 中村 鉄平; 細谷 実里奈; 堀野 太郎; Elewa Yaser Hosny Ali; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 294, 294, 2018年08月
  (公社)日本獣医学会, 日本語
• ヒトコブラクダ甲状腺の形態学的特徴 鰓後体遺残とC細胞の分布
  中村 鉄平; 市居 修; Elewa Ali Elewa Yaser; 細谷 実里奈; Ghonimi Wael A.M.; 辰巳 治; 長崎 健一; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 161回, 292, 292, 2018年08月
  (公社)日本獣医学会, 日本語
• Local CD34-positive capillary decreased with the progression of lesion in respective area of kidney in mice.
  Md. A. Masum; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 17th International Conference on Nephrology and Urology. London, UK. 13th March 2018., 17th, 2018年03月
  英語
• “Dual effects of bleomycin on the intra-thoracic immune hemostasis and lung injury in autoimmune disease model mice.”
  Y. H. A. Elewa; O. Ichii, S; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Kuching, Sarawak, Malaysia., 6th, P-18, 2017年12月
  英語
• BXSB/MpJ-Yaa MICE SHOW THE INCREASE OF APOPTOTIC SPERMATOCYTES IN STAGE Ⅻ SEMINIFEROUS TUBULES.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Kuching, Sarawak, Malaysia. October 12-13, 2017., 6th, 2017年10月
  英語
• ABNOLMALITY OF OVULATION AND OOCYTE-PICK-UP IN MRL/MpJ-Faslpr/lpr MICE.
  Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; S. Otsuka-Kanazawa; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia, 6th, 2017年10月
  英語
• IL-1F6/IL-36α SIGNALING REGULATES RENAL INFLAMMATION.
  O. Ichii; J. Kimura; T. Okamura; T. Horino; T. Nakamura; H. Sasaki; H. A. Elewa; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia, 6th, 2017年10月
  英語
• FAT ACCUMULATION CORRELATES WITH DIABETES MELLITUS IN MALE COTTON RATS (SIGMODON HISPIDUS).
  T. Nakamura; O. Ichii; T. Irie; H. Kouguchi; Y. Sunden; K.-I. Nagasaki; Y. H. A. Elewa; Y. Kon, The 6th Congress of Asian Association of Veterinary Anatomists, Malaysia, 2017年10月
• “Mechanism and abnormality of ovulation and picking up of mammalian oocytes-Immune factors affecting the molecular morphology of the oviduct.”
  M. Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 5th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2017. Sapporo, Japan., 5th, 2017年09月
  英語
• “Loss of endothelial fenestration correlates with podocyte injury in the mouse model of membranoproliferative glomerulonephritis.”
  Md. A. Masum; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 5th Sapporo Summer Seminar for One Health (SASSOH). September 20-21, 2017. Sapporo, Japan., 5th, 2017年09月
  英語
• “The unique phenotype in ovulation and corpus luteum formation in MRL/MpJ mouse.”
  M. Hosotani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 160th Meeting of the Japanese Society of Veterinary Sciences, September 13-16, 2017. Kagoshima, Japan., 160th, 2017年09月
  日本語
• Histopathological features observed in the testis of autoimmune-prone BXSB/MpJ-Yaa mice.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 5th Sapporo Summer Seminar for One Health., 5th, 2017年09月
  英語
• Apoptosis of spermatocytes seen in BXSB / MpJ-Yaa mice - association with autoimmune abnormality.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 160th Meeting of the Japanese Society of Veterinary Sciences., 160th, 2017年09月
  日本語
• “Dual effects of bleomycin on the intra-thoracic immune hemostasis and lung injury in autoimmune disease model mice”.
  Y. H. A. Elewa; T. Nakamura; O. Ichii; Y. Kon, The 63th annual meeting of the Japanese the Anatomical Society., 63th, 2017年09月
  英語
• Caspase3欠損マウスからみる細胞死―自己免疫連関異常の新たなエビデンス
  市居修; 堀野太郎; 大田寛; 鈴木崇史; 中村鉄平; 中村鉄平; ELEWA Yaser; Hosny Ali; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160th, 310, 310, 2017年08月30日
  (公社)日本獣医学会, 日本語
• BXSB/MpJ‐Yaaマウスにみられる精母細胞のアポトーシス―自己免疫異常との関連性―
  大谷祐紀; 市居修; ELEWA Yaser; Hosny Ali; 木村純平; MASUM Md. Abdul; 中村鉄平; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160th, 312, 312, 2017年08月30日
  (公社)日本獣医学会, 日本語
• 雌性コットンラットの胸腺にみられる加齢性変化
  中村鉄平; 中村鉄平; 市居修; 入江隆夫; 篠原明雄; 孝口裕一; 越本知大; 長崎健一; 寸田祐嗣; ELEWA Yaser Hosny; 辰巳治; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160th, 309, 309, 2017年08月30日
  (公社)日本獣医学会, 日本語
• MRL/MpJマウスの排卵―黄体形成過程にみられる特異な表現型
  細谷実里奈; 市居修; ELEWA Yaser; Hosny Ali; 中村鉄平; 中村鉄平; 昆泰寛, 日本獣医学会学術集会講演要旨集, 160th, 313, 313, 2017年08月30日
  (公社)日本獣医学会, 日本語
• “Pathological correlations between endothelial cells and podocytes in autoimmune-related injury of the mouse glomerulus.”
  Md. A. Masum; O. Ichii; Y. H. A. Elewa; T. Nakamura; Y. Kon, The 160th Meeting of the Japanese Society of Veterinary Sciences, September 13-16, 2017. Kagoshima, Japan., 160回, 310, 310, 2017年08月
  (公社)日本獣医学会, 英語
• Elucidation of mammalian reproductive - autoimmune coupling mechanism and analysis of pathological conditions caused by its failure.
  Y. Otani; O. Ichii; Y. H. A. Elewa; T. Nakamura, The 35th Scientific annual joint meeting of Hokkaido Veterinary Anatomy, 35th, 2017年08月
  日本語
• “CD34-positive capillary decreased with the progression of kidney disease in mouse models.”
  Md. A. Masum; Y. H. A. Elewa; T. Nakamura; O. Ichii; Y. Kon, The 159th Meeting of the Japanese Society of Veterinary Sciences, September 6-8, 2016. Kanagawa, Japan., 159th, 2016年09月
  英語
• “Histopathological correlations between the mediastinal fat associated lymphoid clusters and bleomycin-induced pneumonitis in mice.”
  Y. H. A. Elewa; Md. A. Masum; T. Nakamura; O. Ichii; Y. Kon, The 159th Meeting of the Japanese Society of Veterinary Sciences. September 6-8, 2016. Kanagawa, Japan., 159回, 302, 302, 2016年08月
  (公社)日本獣医学会, 英語
• “Histopathological evaluation of the possible protective role of royal jelly and cod liver oil against reproductive toxicity of tartrazine in adult male albino rats.”
  Y. H. A. Elewa; A. Moustafa; A.A. Mohamed; A. A.A. Galal; O. Ichii; Y. Kon, The 8th international Toxicology symposium in Africa. Cairo, Egypt., 8th, 2016年08月
  英語
• マウスにおける縦隔脂肪関連リンパクラスターとブレオマイシン誘発性肺炎との病理組織学的関連(Histopathological correlations between the mediastinal fat associated lymphoid clusters and bleomycin-induced pneumonitis in mice)
  Ali Elewa Yaser Hosny; Masum Md.Abdul; 中村 鉄平; 市居 修; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 159回, 302, 302, 2016年08月
  (公社)日本獣医学会, 英語
• マウス腎臓病モデルにおける疾患進行に伴うCD34陽性毛細血管の減少(CD34-positive capillary decreased with the progression of kidney disease in mouse models)
  Masum Md.Abdul; Yaser Hosny Ali Elewa; 中村 鉄平; 市居 修; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 159回, 308, 308, 2016年08月
  (公社)日本獣医学会, 英語
• 自己免疫疾患モデルMRL/MpJ-Faslprマウスにみられる卵子のキャッチアップ障害
  細谷 実里奈; 中村 鉄平; Hosny Ali Elewa Yaser; 市居 修; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 159回, 314, 314, 2016年08月
  (公社)日本獣医学会, 日本語
• “Sex-related difference of the mediastinal fat associated lymphoid clusters in MRL/MpJ-Faslpr autoimmune disease model mice.”
  Y. H. A. Elewa; O. Ichii; Y. Kon, The 11th Scientific Vet. Med. (second international) conference. El-Ain-El Sokhna, Egypt., 11th, 2016年03月
• Rapid specimen preparation to improve throughput of electron microscopic volume imaging
  THAI Truc Quynh; NGUYEN Huy Bang; SAITOH Sei; SAITOH Yurika; SHIMO Satoshi; ELEWA Yaser; Hosny Ali; ICHII Osamu; KON Yasuhiro; TAKAKI Takashi; JOH Kensuke; OHNO Nobuhiko, 日本顕微鏡学会関東支部講演会予稿集, 40th, 26, 2016年
  英語
• A Novel Mouse Mediastinal Fat-associated Lymphoid Tissue and Its Relationship with Autoimmune Disease
  Y. H. A. Elewa; O. Ichii; S. Otsuka-Kanazaw; Y. Kon, The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA). Bali, Indonesia, 5th, 2015年02月
  英語
• マウス縦隔の脂肪関連リンパ球集積の性状解析(Characterization of mouse mediastinal fat-associated lymphoid clusters)
  Elewa Yaser; Hosny Ali; 市居 修; 大塚 沙織; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 157回, 330, 330, 2014年08月
  (公社)日本獣医学会, 英語
• Ovarian cysts in MRL/MpJ mice are derived from the extraovarian rete: a developmental study (vol 219, pg 743, 2011)
  Shin-Hyo Lee; Osamu Ichii; Saori Otsuka; Yaser Hosny Ali Elewa; Yuka Namiki; Yoshiharu Hashimoto; Yasuhiro Kon, JOURNAL OF ANATOMY, 222, 3, 396, 396, 2013年03月
  英語
• Cytoarchitectural differences of myoepithelial cells among goat major salivary glands (vol 34, pg 557, 2010)
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abuel-Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shin-Hyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon, VETERINARY RESEARCH COMMUNICATIONS, 34, 8, 727, 727, 2010年12月
  英語, その他
• ヤギの大唾液腺における離乳前・後期のアクアポリンファミリー発現の比較解析(Comparative analysis of the expression of aquaporin family between pre- and post-weaning periods in goat major salivary glands)
  Yaser Elewa; 市居 修; 大塚 沙織; 金澤 智則; 李 愼曉; 並木 由佳; 昆 泰寛; 橋本 善春, 日本獣医学会学術集会講演要旨集, 150回, 160, 160, 2010年09月
  (公社)日本獣医学会, 英語
• Structural characteristics of goat (Capra hircus) parotid salivary glands
  Yaser Hosny Elewa; Mohammad Hafez Bareedy; Ahmed Awad Abuel-Atta; Osamu Ichii; Saori Otsuka; Tomonori Kanazawa; Shinhyo Lee; Yoshiharu Hashimoto; Yasuhiro Kon, JAPANESE JOURNAL OF VETERINARY RESEARCH, 58, 2, 121, 135, 2010年08月
  英語
• ヤギ耳下腺の年齢関連の構造変化(Age-related structural changes of parotid salivary glands in goat)
  Elewa Yaser; 橋本 善春; 市居 修; 大塚 沙織; 金澤 智則; 昆 泰寛, 日本獣医学会学術集会講演要旨集, 149回, 211, 211, 2010年03月
  (公社)日本獣医学会, 英語

• Japanese Society for promotion and Sciences (JSPS)- Alumni, Egypt
• Egyptian Society of Veterinary medical sciences
• Egyptian Society of Histology and Cytology
• 日本解剖学会
• 日本獣医学会

• MFALCs免疫細胞のリプログラミング：慢性閉塞性肺疾患（COPD）の革新的治療戦略
  科学研究費助成事業
  2025年04月01日 - 2028年03月31日
  エレワ ヤセル
  日本学術振興会, 基盤研究(C), 北海道大学, 25K11423
• ウシのリンパ陰部輪の発見 ―粘膜ワクチン投与部位としての有効性―
  科学研究費助成事業
  2019年04月01日 - 2024年03月31日
  昆 泰寛; エレワ ヤセル; 市居 修; 中村 鉄平
  鼻咽頭、腸管、気管などの外界と接する部位には粘膜関連リンパ組織が存在し、病原体を含む異物に対し粘膜免疫を活性化させ、さらに全身の免疫反応制御に深く関わっている。昨年度までに、小動物（マウス、コットンラット）や中大動物（ウシ、ヤギ、ミニブタ、イヌ）の生殖器を形態学的に解析し、中大動物の腟前庭部位にリンパ球の散在性あるいは濾胞性集簇が認められることを明らかにし、「生殖器関連リンパ組織（GOALT）」と定義した。
  本年度はウシのモデルとしてヤギを用い、腟前庭にみられるGOALTの構造を中心に精査した。ヤギ（ザーネン種、n＝6、非妊娠、1歳以上）の腟前庭を形態学的に観察した。ホールマウント標本では、腟前庭粘膜全体に大小様々なヘマトキシリン陽性スポットが認められ、組織学的にはリンパ濾胞（LF）や散在性リンパ組織（DLT）からなるリンパ組織（LT）だった。免疫染色においては、腟前庭GOALTのLFで、免疫細胞と高内皮細静脈が観察された。GOALTではCD20陽性B細胞が他の免疫細胞と比較して多く存在し、主にLFの胚中心部に認められた。CD3陽性T細胞とIba1陽性マクロファージは、胚中心およびLFと腟前庭上皮の境界部に観察された。さらに、走査型電子顕微鏡観察では、腟前庭GOALTの直上に位置する重層上皮が部分的に破壊されていた。また、GOALT内の免疫細胞や赤血球は結合組織線維間に局在していた。
  ウシと同様、ヤギの腟前庭におけるGOALTはリンパ咽頭輪と形態学的類似性を示し、生殖器における免疫ゲートを担うと考えられた。また、GOALTが直接腟前庭の管腔内と交通する構造は特徴的であり、産業動物の感染症発生機序の解明や粘膜ワクチン接種の開発において重要な知見となる。
  日本学術振興会, 基盤研究(B), 北海道大学, 19H03113
• 縦隔関連リンパ組織に着目したヒト呼吸器・心血管疾患の革新的な治療戦略研究
  2020年04月 - 2023年04月
  基盤研究(C), 研究代表者
• アダムからイブを作る ー雄ゲノムのみによる個体作出の挑戦ー
  科学研究費助成事業
  2019年06月28日 - 2022年03月31日
  昆 泰寛; エレワ ヤセル; 市居 修; 中村 鉄平
  精巣に出現する卵細胞の意義を解明する、すなわち精子形成細胞が卵細胞に形質転換する機構を検証し、ついには雄ゲノムのみによる個体の作出に挑戦する。本研究では、MRL/MpJマウスの精巣に出現する卵母細胞の分離法を検討し、効率良くかつ形態も保持されたより多くの精巣内卵母細胞を回収する方法を模索した。精巣内卵細胞は用手法で最も効率よく分離され、その形態も維持された。一方、精巣内卵細胞と卵巣内卵細胞の機能的差異の考察や培養法の更なる検討が必要だと考えられた。本研究を発展させることで精巣内卵細胞の受精能や胚発生能が明らかとなり、動物の繁殖、特に家畜産生、生殖工学や希少動物個体保護等に応用されることを望む。
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 19K22409
• 尿路関連リンパ組織の発見から展開する新たな腎泌尿器免疫学
  科学研究費助成事業
  2019年06月28日 - 2022年03月31日
  市居 修; 昆 泰寛; エレワ ヤセル; 中村 鉄平
  局所で発達するリンパ組織は、感染防御において重要な役割を果たす。特に近年、粘膜関連リンパ組織の役割が注目されており、眼、気道や腸管等で発見されている。我々はマウスの腎泌尿器を精査し、新たな免疫関連組織“尿路関連リンパ組織, Urinary Tract-Associated Lymphoid Structure, UTALS”を見出した。これまでUTALSに関する情報は少なく、本研究はその機能や病態意義を追究した。
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 19K22352
• 若手研究(B)
  2017年04月 - 2020年04月
  エレワ ヤセル
  若手研究(B), 自然リンパ球を産生する新たな免疫器官－縦隔内脂肪関連リンパ 組織の機能とその異常－, 研究代表者, 競争的資金
• 自然リンパ球を産生する新たな免疫器官－縦隔内脂肪関連リンパ組織の機能とその異常－
  科学研究費助成事業
  2017年04月01日 - 2020年03月31日
  エレワ ヤセル
  報告者らは、MRL/MpJ-fas lprマウスにおける自己免疫性肺病変の性差を明らかにし、その縦隔関連リンパ組織（MFALCs）との関連性を示し、科学論文として公表した（Elewa et al. 2017, Autoimmunity)。加えて、ブレオマイシン投与マウスの肺病変の病理発生を明らかにし、それらはMFALCsの形態変化と関連することを明らかにし、科学論文として公表した（Elewa et al. 2018, Front. Immunol.）。一方、国内外の研究者と共同研究を進め、筆頭著者として共著論文を公表し(Elewa et al. 2019)、また30篇以上の共著論文を公表した。
  日本学術振興会, 若手研究(B), 北海道大学, 17K15388