研究者基本情報

• https://researchmap.jp/sasakiyuki1120

• https://orcid.org/0000-0002-1953-4366
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202101012774500407

• 00923369

• https://orcid.org/0000-0002-1953-4366

• 202101012774500407

• 唇顎口蓋裂
• 頭蓋顎顔面外科
• 脈管奇形
• 血管腫
• 抗老化
• 老化細胞
• 悪性黒色腫
• 腫瘍溶解性ウイルス
• 羊膜由来間葉系幹細胞
• 間葉系幹細胞
• Klotho
• 再生医療
• 創傷治癒

• ライフサイエンス, 形成外科学

研究活動情報

• 2024年04月, 日本形成外科学会, 2023年度学術奨励賞優秀賞

• Ultra-deep targeted next-generation sequencing with peripheral blood in patients with vascular malformations: a cohort of 40 patients
  Shintaro Mitamura; Kosuke Ishikawa; Kanako C. Hatanaka; Tatsuro Saito; Yuki Sasaki; Takahiro Miura; Taku Maeda; Emi Funayama; Naoki Murao; Satoru Sasaki; Yutaka Hatanaka
  Molecular Biology Reports, 2026年12月
  研究論文（学術雑誌）
• Combined unilateral coronal and intraoral approach with zygomatic arch osteotomy for a dumbbell-shaped atypical lipomatous tumor of the temporal region extending into the infratemporal fossa
  Kosuke Ishikawa; Satoshi Kano; Yuki Sasaki; Keisuke Tada; Masahiro Hojo; Taku Maeda
  Auris Nasus Larynx, 2026年08月
  研究論文（学術雑誌）
• Super-Passive Alveolar Correcting Equipment (SPACE): a novel presurgical cleft device with 5-year outcomes following one-stage repair
  Akihiko Oyama; Emi Funayama; Toru Okamoto; Takahiiro Miura; Yuki Sasaki; Noriko Nishizawa; Satoko Imai; Taku Maeda; Kosuke Ishikawa; Masahiro Hojo; Yuhei Yamamoto
  Journal of Cranio-Maxillofacial Surgery, 2026年08月
  研究論文（学術雑誌）
• Multimodal therapy with percutaneous sclerotherapy, partial excision, and pulsed dye laser for lip arteriovenous malformations: a case series
  K. Ishikawa; S. Mitamura; Y. Sasaki; T. Maeda; N. Murao; S. Sasaki
  International Journal of Oral and Maxillofacial Surgery, 2026年07月
  研究論文（学術雑誌）
• Topical amnion-derived mesenchymal stem cell conditioned medium attenuates chronic ultraviolet B-induced skin photodamage in hairless mice.
  Shintaro Mitamura; Kosuke Ishikawa; Hiroko Takahashi; Yuki Sasaki; Shunsuke Ohnishi; Taku Maeda
  Photochemistry and photobiology, 2026年05月10日, ［国際誌］
  英語, 研究論文（学術雑誌）, Ultraviolet B (UVB) exposure induces epidermal hyperplasia, wrinkle formation, and stress-associated responses; however, practical topical countermeasures remain limited. This exploratory proof-of-concept study evaluated whether topical amnion-derived mesenchymal stem cell conditioned medium (AMSC-CM) attenuates chronic UVB-induced photodamage in hairless mice. Male Hos:HR-1 hairless mice were exposed to escalating UVB doses three times per week for 10 weeks and received no topical treatment, α-minimum essential medium (αMEM) vehicle gel, or AMSC-CM gel immediately after each irradiation session; a nonirradiated group served as a control. Wrinkle severity was graded on endpoint dorsal photographs by a masked evaluator, and skin sections were analyzed for epidermal and dermal thickness and epidermal p21 immunoreactivity. UVB exposure increased wrinkle scores, epidermal thickness, and epidermal p21 positivity relative to nonirradiated controls. Compared with UVB alone, AMSC-CM significantly reduced wrinkle severity (mean score 2.00 vs. 4.50; Holm-adjusted p = 0.035). By contrast, epidermal thickness and epidermal p21 positivity showed directional but nonsignificant reductions after Holm correction. These findings support wrinkle attenuation as the primary statistically supported outcome of this exploratory proof-of-concept study and suggest that topical AMSC-CM may modulate chronic UVB-induced photodamage under controlled dosimetry, while secondary histological and p21-related observations remain preliminary.
• Flap Selection in Pressure Ulcer Reconstruction Using a Longitudinal Archive of Ten Representative Cases for the Board Certification Examination of the Japan Society of Plastic and Reconstructive Surgery
  Kosuke Ishikawa; Yuki Sasaki; Takahiro Miura; Masahiro Hojo; Toshihiko Hayashi; Akihiko Oyama; Hiroshi Furukawa; Chu Kimura; Emi Funayama; Taku Maeda
  International Journal of Surgical Wound Care, 2025年12月01日
  研究論文（学術雑誌）
• Extracellular matrix modulating effects of amnion‐derived mesenchymal stem cells on aging skin wounds in α‐Klotho knockout mice
  Yuki Sasaki; Shunsuke Ohnishi; Hiroko Takahashi; Kosuke Ishikawa; Takahiro Miura; Emi Funayama; Naoto Okubo; Yuhei Yamamoto; Taku Maeda
  Geriatrics & Gerontology International, 25, 5, 701, 708, Wiley, 2025年04月14日
  研究論文（学術雑誌）, Aim
  
  Wounds in the elderly are frequently recalcitrant and chronic as a result of the effects of skin aging and associated complications. The objective of this study is to utilize an α‐Klotho knockout (KO) mice wound model to assess the capacity of amnion‐derived mesenchymal stem cells (AMSCs) to facilitate wound healing in aging skin.
  
  Methods
  
  AMSCs were applied topically to the wound after extraction and gelatinization of the conditioned medium (CM). Animal experiments were performed with two distinct mouse strains: α‐Klotho KO mice and wild‐type mice. Full‐thickness skin defect models with a diameter of 8 mm were created by incising the skin on the left and right sides of the dorsum. On day 8 after wound creation, the mice were sacrificed, and wound tissue was collected for analysis through histological and immunohistochemical evaluations, as well as through quantitative polymerase chain reaction.
  
  Results
  
  The topical application of CM gel to wounds of α‐Klotho KO mice demonstrated that wound healing was significantly higher than that observed in control, reaching the wound closure rate of wild‐type mice on day 8. Additionally, gene expression analysis of wound tissue indicated that AMSC‐CM may regulate extracellular matrix formation and fibrosis. Moreover, histological analysis indicated that AMSC‐CM may facilitate wound contraction of aging skin wounds of α‐Klotho KO mice by inducing myofibroblast differentiation and promoting granulation and collagen formation, which are the primary components of the extracellular matrix.
  
  Conclusions
  
  AMSC‐CM may facilitate wound healing in aging skin of α‐Klotho KO mice by regulating the extracellular matrix. Geriatr Gerontol Int 2025; 25: 701–708.
• Pitfalls in Ultrasound Diagnosis of Vascular Malformations: A Retrospective Review of 14 Nonvascular Tumors Treated as Vascular Malformations
  Shintaro Mitamura; Kosuke Ishikawa; Yuki Sasaki; Naoki Murao; Satoru Sasaki
  Diagnostics, 2025年02月19日
  研究論文（学術雑誌）
• Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitor Roxadustat Accelerates Wound Healing in a Mouse Hind limb Lymphedema Model.
  Kosuke Ishikawa; Yoshitada Hoshino; Masayuki Osawa; Emi Funayama; Takahiro Miura; Masahiro Hojo; Yuki Sasaki; Satoru Sasaki; Yuhei Yamamoto; Taku Maeda
  Advances in wound care, 2025年01月24日, ［国際誌］
  英語, 研究論文（学術雑誌）, Objective: Drugs regulating hypoxia-inducible factor (HIF)-1α have not been investigated for wound healing in lymphedema. Therefore, we examined the effects of drug modulation of HIF-1α activity for wound healing in our previously developed mouse model of nonirradiated hind limb lymphedema. Approach: Mouse hind limb lymphedema models (n = 17) and a sham group (n = 6) were created using 8- to 10-week-old male C57BL/6N mice. Mice with hind limb lymphedema were randomized into experimental groups receiving roxadustat, 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1), or dimethyl sulfoxide and were given intraperitoneal injections every 2 days for up to 2 weeks. Four days after the surgery, an 8-mm diameter full-thickness skin wound was created in the hind limb. The number of days required for wound closure and the percentage of wounds closed were measured. Skin samples taken at wound creation were evaluated by histological and molecular analysis. Results: Administration of roxadustat accelerated wound healing, whereas YC-1 delayed it, with a significant decrease and increase in skin thickness, respectively. The relative mRNA expression of Hif1α, matrix metalloproteinase-3, and interleukin-6 was significantly higher in the roxadustat group and that of metalloproteinase-9 was significantly lower in the roxadustat group compared with the control group. Innovation: This study is the first to demonstrate delayed wound healing in a mouse model of hind limb lymphedema and the first to demonstrate the promotion of significant wound healing through the use of roxadustat. Conclusion: Roxadustat exerts wound-healing effects and may promote the regulation of extracellular matrix remodeling via gene expression in hind limb lymphedema wound models.
• Synergistic anti-tumor effects of oncolytic virus and anti-programmed cell death protein 1 antibody combination therapy: For suppression of lymph node and distant metastasis in a murine melanoma model
  Yuki Sasaki; Taku Maeda; Masahiro Hojo; Takahiro Miura; Kosuke Ishikawa; Emi Funayama; Kazufumi Okada; Yuhei Yamamoto
  Biochemical and Biophysical Research Communications, 740, 151011, 151011, Elsevier BV, 2024年12月
  研究論文（学術雑誌）
• Fluoroscopy- and Endoscopy-Guided Transoral Sclerotherapy Using Foamed Polidocanol for Oropharyngolaryngeal Venous Malformations in a Hybrid Operation Room: A Case Series
  Kosuke Ishikawa; Taku Maeda; Emi Funayama; Naoki Murao; Takahiro Miura; Yuki Sasaki; Dongkyung Seo; Shintaro Mitamura; Shunichi Oide; Yuhei Yamamoto; Satoru Sasaki
  Journal of Clinical Medicine, 2024年04月18日
  研究論文（学術雑誌）
• Intranodal lymphangiography with lipiodol as a diagnostic and therapeutic approach for spontaneous cervical chyle leak
  Yuki Sasaki; Yusuke Sakuhara; Satoru Sasaki; Taku Maeda; Yuhei Yamamoto; Kosuke Ishikawa
  Clinical Case Reports, 11, 12, Wiley, 2023年12月19日
  研究論文（学術雑誌）, Key Clinical Message
  
  We present a case of spontaneous cervical chyle leak that showed as left‐sided neck swelling. Spontaneous chyle leak is extremely rare. Lymphangiography with lipiodol is useful as a diagnostic and therapeutic approach for chyle leak.
• Partially involuting congenital hemangioma with pyogenic granuloma‐induced rapid progression following incisional biopsy in infancy: A case report
  Yuki Sasaki; Kosuke Ishikawa; Takahiro Miura; Emi Funayama; Yuhei Yamamoto; Taku Maeda
  Clinical Case Reports, 11, 10, Wiley, 2023年10月17日
  研究論文（学術雑誌）, Key Clinical Message
  
  We present a case of partially involuting congenital hemangioma (PICH) that showed rapid growth with ulceration after incisional biopsy. PICH does not typically grow after birth. However, if growth occurs due to stimuli like biopsy, it is essential to consider the possibility of pyogenic granuloma complication.
• Targeted next-generation sequencing for detection of PIK3CA mutations in archival tissues from patients with Klippel–Trenaunay syndrome in an Asian population
  Yuki Sasaki; Kosuke Ishikawa; Kanako C. Hatanaka; Yumiko Oyamada; Yusuke Sakuhara; Tadashi Shimizu; Tatsuro Saito; Naoki Murao; Tomohiro Onodera; Takahiro Miura; Taku Maeda; Emi Funayama; Yutaka Hatanaka; Yuhei Yamamoto; Satoru Sasaki
  Orphanet Journal of Rare Diseases, 18, 1, Springer Science and Business Media LLC, 2023年09月04日
  研究論文（学術雑誌）, Abstract
  
  Background
  
  Klippel–Trenaunay syndrome (KTS) is a rare slow-flow combined vascular malformation with limb hypertrophy. KTS is thought to lie on the PIK3CA-related overgrowth spectrum, but reports are limited. PIK3CA encodes p110α, a catalytic subunit of phosphatidylinositol 3-kinase (PI3K) that plays an essential role in the PI3K/AKT/mammalian target of rapamycin (mTOR) signaling pathway. We aimed to demonstrate the clinical utility of targeted next-generation sequencing (NGS) in identifying PIK3CA mosaicism in archival formalin-fixed paraffin-embedded (FFPE) tissues from patients with KTS.
  
  Results
  
  Participants were 9 female and 5 male patients with KTS diagnosed as capillaro-venous malformation (CVM) or capillaro-lymphatico-venous malformation (CLVM). Median age at resection was 14 years (range, 5–57 years). Median archival period before DNA extraction from FFPE tissues was 5.4 years (range, 3–7 years). NGS-based sequencing of PIK3CA achieved an amplicon mean coverage of 119,000x. PIK3CA missense mutations were found in 12 of 14 patients (85.7%; 6/8 CVM and 6/6 CLVM), with 8 patients showing the hotspot variants E542K, E545K, H1047R, and H1047L. The non-hotspot PIK3CA variants C420R, Q546K, and Q546R were identified in 4 patients. Overall, the mean variant allele frequency for identified PIK3CA variants was 6.9% (range, 1.6–17.4%). All patients with geographic capillary malformation, histopathological lymphatic malformation or macrodactyly of the foot had PIK3CA variants. No genotype–phenotype association between hotspot and non-hotspot PIK3CA variants was found. Histologically, the vessels and adipose tissues of the lesions showed phosphorylation of the proteins in the PI3K/AKT/mTOR signaling pathway, including p-AKT, p-mTOR, and p-4EBP1.
  
  Conclusions
  
  The PI3K/AKT/mTOR pathway in mesenchymal tissues was activated in patients with KTS. Amplicon-based targeted NGS could identify low-level mosaicism from low-input DNA extracted from FFPE tissues, potentially providing a diagnostic option for personalized medicine with inhibitors of the PI3K/AKT/mTOR signaling pathway.
• 皮膚欠損用創傷被覆材Sorbactコンプレスを用いた広範囲熱傷デブリードマン後の局所療法
  石川 耕資; 北條 正洋; 星野 善允; 石井 浩子; 石井 陸; 佐々木 雄輝; 徐 東經; 松田 識郁; 三浦 隆洋; 前田 拓; 舟山 恵美; 山本 有平
  熱傷, 49, 2, 103, 103, (一社)日本熱傷学会, 2023年06月
  日本語
• Effectiveness and safety of percutaneous sclerotherapy using absolute ethanol and/or polidocanol for maxillofacial venous malformations involving the masticatory muscles: A case series
  Kosuke Ishikawa; Satoru Sasaki; Hiroshi Furukawa; Taku Maeda; Takahiro Miura; Yuki Sasaki; Yuhei Yamamoto; Emi Funayama
  Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 135, 3, 355, 362, Elsevier BV, 2023年03月
  研究論文（学術雑誌）
• 出血を伴う舌動静脈奇形に対し腸鉗子による血流コントロールを併用してポリドカノール硬化療法を行った一例
  佐々木 雄輝; 石川 耕資; 村尾 尚規; 西尾 卓哉; 福田 摩莉佳; 箱崎 茉衣; 櫻井 優; 佐々木 了
  日本頭蓋顎顔面外科学会学術集会プログラム・抄録集, 40回, 141, 141, (一社)日本頭蓋顎顔面外科学会, 2022年11月
  日本語
• 耳後部に発生した好酸球性血管リンパ球増殖症の一例
  本田 進; 松田 識郁; 石井 陸; 木村 中; 佐々木 雄輝; 前田 拓; 田中 敏
  日本形成外科学会会誌, 42, 6, 361, 361, (一社)日本形成外科学会, 2022年06月
  日本語
• 口腔咽頭静脈奇形に対するハイブリッド手術室を使用した内視鏡補助下透視下硬化療法の検討
  石川 耕資; 村尾 尚規; 佐々木 雄輝; 西尾 卓哉; 福田 摩莉佳; 三田村 真太郎; 竹田 朋弘; 藤田 宗純; 佐々木 了
  日本頭蓋顎顔面外科学会学術集会プログラム・抄録集, 39回, 118, 118, (一社)日本頭蓋顎顔面外科学会, 2021年11月
  日本語
• Lingual Arteriovenous Malformation With Bleeding Treated With Polidocanol Foam Sclerotherapy Using Intestinal Forceps to Control Blood Flow.
  Yuki Sasaki; Satoru Sasaki; Kosuke Ishikawa
  The Journal of craniofacial surgery, 33, 3, e292-e293, 2021年09月10日, ［国際誌］
  英語, 研究論文（学術雑誌）, ABSTRACT: Arteriovenous malformation (AVM) sometimes causes hemorrhage that can be fatal. We report a case of AVM of the tongue with bleeding that was treated by semi-emergent sclerotherapy with polidocanol. A 33-year-old woman presented with Schobinger stage III AVM of the tongue. Sclerotherapy with 3% polidocanol foam was performed under general anesthesia using curved intestinal forceps to clamp the root of the tongue for control of blood flow. Postoperatively, there was no further bleeding from the lesion. Three subsequent sclerotherapy sessions with polidocanol were performed, and there was a marked reduction in the size of the lesion. The lesion has remained well controlled in the year since the last sclerotherapy session.

• 足底Malignant mixed tumor of the skin再発の1例
  佐々木雄輝; 前田拓; 北條正洋; 石川耕資; 舟山恵美; 山本有平; 林利彦, 日本形成外科学会総会・学術集会プログラム・抄録集, 66th, 2023年
• 胼胝として受診した足底部Malignant mixed tumor of the skinの1例
  佐々木雄輝; 前田拓; 北條正洋; 三浦隆洋; 石川耕資; 舟山恵美; 山本有平; 林利彦, 日本創傷外科学会総会・学術集会プログラム・抄録集, 15th, 2023年
• 低酸素誘導因子活性調整薬を用いたマウス後肢リンパ浮腫モデルにおける創傷治癒の検討
  石川耕資; 星野善允; 前田拓; 佐々木雄輝; 北條正洋; 三浦隆洋; 大澤昌之; 舟山恵美; 山本有平, 日本形成外科学会基礎学術集会プログラム・抄録集, 32nd, 2023年
• 【まずはここから!四肢のしこり診療ガイド】脈管奇形、血管性腫瘍
  佐々木 雄輝; 石川 耕資; 佐々木 了, PEPARS, 181, 20, 33, 2022年01月
  (株)全日本病院出版会, 日本語
• 遊離広背筋弁で再建した頭蓋骨に及ぶIII度熱傷の1例
  竹田 朋弘; 石川 耕資; 林 利彦; 前田 拓; 石井 陸; 佐々木 雄輝; 北條 正洋; 三浦 隆洋; 大澤 昌之; 舟山 恵美; 山本 有平, 熱傷, 47, 3, 114, 121, 2021年09月
  (一社)日本熱傷学会, 日本語
• 下顎癌再建後の上半身熱傷の治療経験
  石川 耕資; 伊藤 梨里; 草島 英梨香; 三浦 隆洋; 北條 正洋; 星野 善允; 石井 陸; 佐々木 雄輝; 竹田 朋弘; 山本 有平, 熱傷, 47, 1, 36, 36, 2021年03月
  (一社)日本熱傷学会, 日本語
• 遊離広背筋弁による再建を要した頭部III度熱傷の1例
  竹田 朋弘; 石川 耕資; 林 利彦; 前田 拓; 伊藤 梨里; 北條 正洋; 石井 陸; 佐々木 雄輝; 山本 有平, 熱傷, 47, 1, 36, 36, 2021年03月
  (一社)日本熱傷学会, 日本語
• 遊離筋皮弁で再建した糖尿病足潰瘍症例の検討
  齋藤 典子; 堀内 勝己; 川嶋 邦裕; 吉田 哲憲; 西尾 卓哉; 佐々木 雄輝; 徐 東經, 形成外科, 63, 8, 1027, 1034, 2020年08月
  遊離筋皮弁移植を行った糖尿病足潰瘍24例(男23例、女1例、平均年齢57.9歳)について検討した。初診時のHbA1c値は平均9.3%で、使用した皮弁は遊離前外側大腿皮弁15例、遊離広背筋弁9例であり、CT、血管造影による血流評価で虚血を認めた12例のうち、3例は血管内治療を、他の9例はバイパス手術を先行し、動脈では端側吻合を行った。その結果、皮弁は虚血のない12例、血管内治療後に再建した3例、バイパス術後に再建した7例の計22例(92%)で生着した。残りの2例は糖尿病性腎症による透析例で、バイパス血管を移植床にして遊離筋弁移植術を行い、いずれも一旦は生着したもののバイパス血管の閉塞により広背筋弁が壊死した。皮弁の壊死には石灰化よりも虚血が深く関与していると考えられ、術前には血流評価を行い、虚血例では筋皮弁手術に先行して血行再建を行うことが重要であることが示唆された。, 克誠堂出版(株), 日本語
• 膝周囲の虚血性潰瘍に対する自家静脈移植と遊離広背筋移植の同時再建術に際して術中血流評価を行った1例
  齋藤 典子; 堀内 勝己; 川嶋 邦裕; 吉田 哲憲; 村尾 尚規; 西尾 卓哉; 佐々木 雄輝, 日本形成外科学会会誌, 40, 7, 360, 366, 2020年07月
  症例は71歳男性で、当科受診7ヵ月前、打撲を契機に右下腿瘢痕に潰瘍が出現した。近医整形外科を経て、前医形成外科を受診した。同医での造影CTにてバイパス血管の血流は保たれているものの、膝窩動脈の閉塞、膝周囲の血流不全を認めた。大腿切断によるADLの著明な低下が危惧されたため下肢温存を目的に当科を紹介された。膝周囲の血流改善と潰瘍閉鎖目的に、自家静脈移植術と遊離広背筋弁手術を計画した。また、本術式により遊離筋弁を含めた膝周囲の血流が増加することで足部への血流が低下する可能性があるため、血流計を用いて術中に血流評価を行う方針とした。自家静脈移植後、移植自家静脈、胸背動脈、膝窩動脈の血流が保たれていることを確認し、遊離広背筋弁手術を行った。術後2年3ヵ月現在、筋弁・分層植皮とも良好に経過し、自力歩行で外来通院している。, (一社)日本形成外科学会, 日本語
• 当院における洗浄液周期的自動注入機能付き陰圧創傷治療システムの使用経験
  本田 進; 木村 中; 佐々木 雄輝; 北條 正洋, 道南医学会大会並びに総会プログラム・抄録集, 71回, 74, 74, 2018年11月
  道南医学会, 日本語
• 重症下肢虚血に対する遠位バイパス手術と遊離筋皮弁のハイブリッド治療
  堀内 勝己; 齋藤 典子; 檀浦 裕; 佐々木 雄輝; 村尾 尚規; 齋藤 達弥; 川嶋 邦弘; 吉田 哲憲, 日本下肢救済・足病学会誌, 10, 2, 161, 161, 2018年07月
  日本下肢救済・足病学会, 日本語
• 手稲渓仁会病院形成外科における眼窩骨折に対するスーパーフィクソーブの使用経験
  大澤 昌之; 七戸 龍司; 佐々木 雄輝; 山本 有平, 口腔顎顔面外傷, 16, 2, 81, 81, 2017年10月
  日本口腔顎顔面外傷学会, 日本語

• 2026年06月 - 現在
  日本人類遺伝学会
• 2026年06月 - 現在
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