Chelatase activity of magnesium dechelatase associated with chlorophyll degradation
Soma Sato, Mitsuaki Hirose, Hitoshi Tamiaki, Hisashi Ito
FEBS Letters, Wiley, 2025年06月17日, ［査読有り］, ［責任著者］
研究論文（学術雑誌）, Metalated tetrapyrrole molecules play crucial roles in respiration, photosynthesis, and biocatalysis. In this study, the chelatase activity of the bacterial magnesium dechelatase homologous gene, which encodes an enzyme that extracts the central magnesium ion from chlorophyll, was demonstrated. The recombinant protein inserted metal ions, such as zinc, into methyl pyropheophorbide a, a synthetic derivative of chlorophyll a. Mutation analysis and structural modeling suggested H32, D34, and D62 as key residues involved in coordinating metal ions and facilitating proton extraction from methyl pyropheophorbide a. Chelatase activity was also found in the recombinant plant magnesium dechelatase protein. The bacterial gene complemented Escherichia coli ferrochelatase mutants. These findings provide novel insights into the mechanisms underlying chelation reactions.

Activity examination of plant Mg-dechelatase and its bacterial homolog in plants and in vitro
Saki Ando, Ryouichi Tanaka, Hisashi Ito
Plant Physiology and Biochemistry, 2024年10月, ［査読有り］, ［責任著者］
研究論文（学術雑誌）

Structural Characterization of the Chlorophyllide a Oxygenase (CAO) Enzyme Through an In Silico Approach
Dey, D., Tanaka, R., Ito, H.
Journal of Molecular Evolution, 91, 2, 2023年, ［査読有り］, ［責任著者］
研究論文（学術雑誌）

Heterologous complementation systems verify the mosaic distribution of three distinct protoporphyrinogen IX oxidase in the cyanobacterial phylum
Ryoya Kohata, HyunSeok Lim, Yuki Kanamoto, Akio Murakami, Yuichi Fujita, Ayumi Tanaka, Wesley Swingley, Hisashi Ito, Ryouichi Tanaka
Journal of Plant Research, 136, 1, 107, 115, Springer Science and Business Media LLC, 2022年11月10日, ［査読有り］
英語, 研究論文（学術雑誌）

Poplar leaf abscission through induced chlorophyll breakdown by Mg-dechelatase
Hisashi Ito, Hideyuki Saito, Manabu Fukui, Ayumi Tanaka, Keita Arakawa
Plant Science, 324, 111444, 111444, Elsevier BV, 2022年11月, ［査読有り］, ［責任著者］
研究論文（学術雑誌）

Crystal structure and reaction mechanism of a bacterial Mg-dechelatase homolog from the Chloroflexi Anaerolineae.
Debayan Dey, Masayoshi Nishijima, Ryouichi Tanaka, Genji Kurisu, Hideaki Tanaka, Hisashi Ito
Protein science : a publication of the Protein Society, 31, 10, e4430, 2022年10月, ［査読有り］, ［責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Chlorophyll degradation plays a myriad of physiological roles in photosynthetic organisms, including acclimation to light environment and nutrient remobilization during senescence. Mg extraction from chlorophyll a is the first and committed step of the chlorophyll degradation pathway. This reaction is catalyzed by the Mg-dechelatase enzyme encoded by Stay-Green (SGR). The reaction mechanism of SGR protein remains elusive since metal ion extraction from organic molecules is not a common enzymatic reaction. Additionally, experimentally derived structural information about SGR or its homologs has not yet been reported. In this study, the crystal structure of the SGR homolog from Anaerolineae bacterium was determined using the molecular replacement method at 1.85 Å resolution. Our previous study showed that three residues-H32, D34, and D62 are essential for the catalytic activity of the enzyme. Biochemical analysis involving mutants of D34 residue further strengthened its importance in the functioning of the dechelatase. Docking simulation also revealed the interaction between the D34 side chain and central Mg ion of chlorophyll a. Structural analysis showed the arrangement of D34/H32/D62 in the form of a catalytic triad that is generally found in hydrolases. The probable reaction mechanism suggests that deprotonated D34 side chain coordinates and destabilizes Mg, resulting in Mg extraction. Besides, H32 possibly acts as a general base catalyst and D62 facilitates H32 to be a better proton acceptor. Taken together, the reaction mechanism of SGR partially mirrors the one observed in hydrolases.

Characterization of photosystem II assembly complexes containing ONE-HELIX PROTEIN1 in Arabidopsis thaliana.
Hanaki Maeda, Koharu Takahashi, Yoshifumi Ueno, Kei Sakata, Akari Yokoyama, Kozue Yarimizu, Fumiyoshi Myouga, Kazuo Shinozaki, Shin-Ichiro Ozawa, Yuichiro Takahashi, Ayumi Tanaka, Hisashi Ito, Seiji Akimoto, Atsushi Takabayashi, Ryouichi Tanaka
Journal of plant research, 135, 2, 361, 376, 2022年03月, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, The assembly process of photosystem II (PSII) requires several auxiliary proteins to form assembly intermediates. In plants, early assembly intermediates comprise D1 and D2 subunits of PSII together with a few auxiliary proteins including at least ONE-HELIX PROTEIN1 (OHP1), OHP2, and HIGH-CHLOROPHYLL FLUORESCENCE 244 (HCF244) proteins. Herein, we report the basic characterization of the assembling intermediates, which we purified from Arabidopsis transgenic plants overexpressing a tagged OHP1 protein and named the OHP1 complexes. We analyzed two major forms of OHP1 complexes by mass spectrometry, which revealed that the complexes consist of OHP1, OHP2, and HCF244 in addition to the PSII subunits D1, D2, and cytochrome b559. Analysis of chlorophyll fluorescence showed that a major form of the complex binds chlorophyll a and carotenoids and performs quenching with a time constant of 420 ps. To identify the localization of the auxiliary proteins, we solubilized thylakoid membranes using a digitonin derivative, glycodiosgenin, and separated them into three fractions by ultracentrifugation, and detected these proteins in the loose pellet containing the stroma lamellae and the grana margins together with two chlorophyll biosynthesis enzymes. The results indicated that chlorophyll biosynthesis and assembly may take place in the same compartments of thylakoid membranes. Inducible suppression of the OHP2 mRNA substantially decreased the OHP2 protein in mature Arabidopsis leaves without a significant reduction in the maximum quantum yield of PSII under low-light conditions, but it compromised the yields under high-light conditions. This implies that the auxiliary protein is required for acclimation to high-light conditions.

Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions.
Koki Fukura, Ayumi Tanaka, Ryouichi Tanaka, Hisashi Ito
Journal of plant physiology, 266, 153535, 153535, 2021年09月25日, ［査読有り］, ［責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, During leaf senescence, chlorophyll a and b are degraded through several enzymatic reactions, including chlorophyll b reductase, 7-hydroxymethyl chlorophyll a reductase, and Mg-dechelatase. Considering that the intermediates of the chlorophyll breakdown pathway are highly photoreactive, cooperative and efficient reactions of chlorophyll metabolic enzymes may protect chloroplasts from potential photo-oxidative damage. Here, we investigated the sub-organellar localization and cooperative reactions of the enzymes involved in the chlorophyll breakdown pathway by the fractionation of thylakoid membranes and enzymatic assays using recombinant proteins. We found that these enzymes were enriched in the grana margin fraction. Furthermore, we found that chlorophyll b reductase and Mg-dechelatase efficiently catabolized chlorophylls bound to the chlorophyll-protein complexes when these two enzymes were mixed. These results suggest that the co-localization of chlorophyll catabolic enzymes enables efficient chlorophyll breakdown. The results from this study highlight a key step forward in the investigation of the photosystem breakdown process.

Distribution and functional analysis of the two types of 8-vinyl reductase involved in chlorophyll biosynthesis in marine cyanobacteria.
Haruka Suehiro, Ryouichi Tanaka, Hisashi Ito
Archives of microbiology, 203, 6, 3565, 3575, 2021年08月, ［査読有り］, ［責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, In the chlorophyll biosynthesis pathway, the 8-vinyl group of the chlorophyll precursor is reduced to an ethyl group by 8-vinyl reductase. Two isozymes of 8-vinyl reductase have been described in oxygenic photosynthetic organisms: one encoded by BciA and another by BciB. Only BciB contains an [Fe-S] cluster and most cyanobacteria harbor this form; whereas a few contain BciA. Given this disparity in distribution, cyanobacterial BciA has remained largely overlooked, which has limited understanding of chlorophyll biosynthesis in these microorganisms. Here, we reveal that cyanobacterial BciA encodes a functional 8-vinyl reductase, as evidenced by measuring the in vitro activity of recombinant Synechococcus and Acaryochloris BciA. Genomic comparison revealed that BciB had been replaced by BciA during evolution of the marine cyanobacterium Synechococcus, and coincided with replacement of Fe-superoxide dismutase (SOD) with Ni-SOD. These findings imply that the acquisition of BciA confers an adaptive advantage to cyanobacteria living in low-iron oceanic environments.

Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis.
Ying Chen, Wataru Yamori, Ayumi Tanaka, Ryouichi Tanaka, Hisashi Ito
Plant science : an international journal of experimental plant biology, 307, 110902, 110902, 2021年06月, ［査読有り］, ［責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, During leaf senescence, the degradation of photosystems and photosynthetic pigments proceeds in a coordinated manner, which would minimize the potential photodamage to cells. Both photosystem I and II are composed of core complexes and peripheral antenna complexes, with the former binding chlorophyll a and the latter binding chlorophyll a and b. Although the degradation of peripheral antenna complexes is initiated by chlorophyll degradation, it remains unclear whether the degradation of core complexes and chlorophyll is coordinated. In this study, we examined the degradation of peripheral antenna and core complexes in the Arabidopsis sgr1/sgr2/sgrl triple mutant, lacking all the isoforms of chlorophyll a:Mg2+ dechelatase. In this mutant, the degradation of peripheral antenna complexes and photosystem I core complexes was substantially retarded, but the core complexes of photosystem II were rapidly degraded during leaf senescence. On the contrary, the photosynthetic activity declined at a similar rate as in the wild type plants. These results suggest that the degradation of photosystem II core complexes is regulated independently of the major chlorophyll degradation pathway mediated by the dechelatase. The study should contribute to the understanding of the complex molecular mechanisms underlying the degradation of photosystems, which is an essential step during leaf senescence.

Insights into the structure and function of the rate-limiting enzyme of chlorophyll degradation through analysis of a bacterial Mg-dechelatase homolog
Debayan Dey, Dipanjana Dhar, Helena Fortunato, Daichi Obata, Ayumi Tanaka, Ryouichi Tanaka, Soumalee Basu, Hisashi Ito
Computational and Structural Biotechnology Journal, 19, 5333, 5347, 2021年01月, ［査読有り］, ［責任著者］
研究論文（学術雑誌）

Natural variations at the Stay-Green gene promoter control lifespan and yield in rice cultivars.
Dongjin Shin, Sichul Lee, Tae-Heon Kim, Jong-Hee Lee, Joonheum Park, Jinwon Lee, Ji Yoon Lee, Lae-Hyeon Cho, Jae Young Choi, Wonhee Lee, Ji-Hwan Park, Dae-Woo Lee, Hisashi Ito, Dae Heon Kim, Ayumi Tanaka, Jun-Hyeon Cho, You-Chun Song, Daehee Hwang, Michael D Purugganan, Jong-Seong Jeon, Gynheung An, Hong Gil Nam
Nature communications, 11, 1, 2819, 2819, 2020年06月04日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Increased grain yield will be critical to meet the growing demand for food, and could be achieved by delaying crop senescence. Here, via quantitative trait locus (QTL) mapping, we uncover the genetic basis underlying distinct life cycles and senescence patterns of two rice subspecies, indica and japonica. Promoter variations in the Stay-Green (OsSGR) gene encoding the chlorophyll-degrading Mg++-dechelatase were found to trigger higher and earlier induction of OsSGR in indica, which accelerated senescence of indica rice cultivars. The indica-type promoter is present in a progenitor subspecies O. nivara and thus was acquired early during the evolution of rapid cycling trait in rice subspecies. Japonica OsSGR alleles introgressed into indica-type cultivars in Korean rice fields lead to delayed senescence, with increased grain yield and enhanced photosynthetic competence. Taken together, these data establish that naturally occurring OsSGR promoter and related lifespan variations can be exploited in breeding programs to augment rice yield.

In vitro enzymatic activity assays implicates the existence of the chlorophyll cycle in chlorophyll b-containing cyanobacteria.
Lim H, Tanaka A, Tanaka R, Ito H
Plant & cell physiology, 2019年08月, ［査読有り］, ［責任著者］

Horizontal transfer of promiscuous activity from non-photosynthetic bacteria contributed to evolution of chlorophyll degradation pathway.
Obata D, Takabayashi A, Tanaka R, Tanaka A, Ito H
Molecular biology and evolution, 2019年08月, ［査読有り］, ［責任著者］

Jasmonate production through chlorophyll a degradation by Stay-Green in Arabidopsis thaliana.
Ono K, Kimura M, Matsuura H, Tanaka A, Ito H
Journal of plant physiology, 238, 53, 62, 2019年07月, ［査読有り］, ［責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Leaf color change through chlorophyll degradation is a characteristic symptom of senescence. Magnesium removal from chlorophyll a is the initial step in chlorophyll a degradation, in a reaction catalyzed by Stay-Green (SGR). Arabidopsis thaliana has three SGR homologs, SGR1, SGR2, and SGR-like. When SGR1 is overexpressed, both chlorophyll a and b are degraded and leaves turn yellow. This process is visually identical to senescence, suggesting that SGR1 overexpression affects various physiological processes in plants. To examine this possibility, gene expression associated with chlorophyll metabolism and senescence was analyzed following dexamethasone-inducible SGR1 introduction into Arabidopsis. When SGR1 was overexpressed following 18 h of dexamethasone treatment, genes involved in chlorophyll degradation were upregulated, as were senescence-associated genes. These observations suggested that chlorophyll a degradation promotes senescence. As jasmonate is the plant hormone responsible for senescence and was expected to be involved in the regulation of gene expression after dexamethasone treatment, the level of jasmonoyl-isoleucine, the active form of jasmonate, was measured. The jasmonoyl-isoleucine level increased slightly after 10 h of SGR1 overexpression, and this increase became significant after 18 h. These observations suggest that jasmonate is produced through chlorophyll a degradation and affects the promotion of senescence.

Mg-dechelatase is involved in the formation of photosystem II but not in chlorophyll degradation in Chlamydomonas reinhardtii.
Chen Y, Shimoda Y, Yokono M, Ito H, Tanaka A
The Plant journal : for cell and molecular biology, 97, 6, 1022, 1031, 2019年03月, ［査読有り］, ［責任著者］

Mg-dechelation of chlorophyll a by Stay-Green activates chlorophyll b degradation through expressing Non-Yellow Coloring 1 in Arabidopsis thaliana
Tomoaki Sato, Yousuke Shimoda, Kaori Matsuda, Ayumi Tanaka, Hisashi Ito
Journal of Plant Physiology, 222, 94, 102, Elsevier GmbH, 2018年03月01日, ［査読有り］, ［責任著者］
英語, 研究論文（学術雑誌）

The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II
Kaori Kohzuma, Yutaka Sato, Hisashi Ito, Ayako Okuzaki, Mai Watanabe, Hideki Kobayashi, Michiharu Nakano, Hiroshi Yamatani, Yu Masuda, Yumi Nagashima, Hiroyuki Fukuoka, Tetsuya Yamada, Akira Kanazawa, Keisuke Kitamura, Yutaka Tabei, Masahiko Ikeuchi, Wataru Sakamoto, Ayumi Tanaka, Makoto Kusaba
PLANT PHYSIOLOGY, 173, 4, 2138, 2147, 2017年04月, ［査読有り］
英語, 研究論文（学術雑誌）

Chlorophyll a is a favorable substrate for Chlamydomonas Mg-dechelatase encoded by STAY-GREEN
Kaori Matsuda, Yousuke Shimoda, Ayumi Tanaka, Hisashi Ito
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 109, 365, 373, 2016年12月, ［査読有り］, ［責任著者］
英語, 研究論文（学術雑誌）

Simultaneous regulation of antenna size and photosystem I/II stoichiometry in Arabidopsis thaliana
Ting Jia, Hisashi Ito, Ayumi Tanaka
PLANTA, 244, 5, 1041, 1053, 2016年11月, ［査読有り］, ［責任著者］
英語, 研究論文（学術雑誌）

Arabidopsis STAY-GREEN, Mendel's Green Cotyledon Gene, Encodes Magnesium-Dechelatase
Yousuke Shimoda, Hisashi Ito, Ayumi Tanaka
PLANT CELL, 28, 9, 2147, 2160, 2016年09月, ［査読有り］
英語, 研究論文（学術雑誌）

Chlorophyll b degradation by chlorophyll b reductase under high-light conditions
Rei Sato, Hisashi Ito, Ayumi Tanaka
PHOTOSYNTHESIS RESEARCH, 126, 2-3, 249, 259, 2015年12月, ［査読有り］
英語, 研究論文（学術雑誌）

Functional characterization of the FNT family nitrite transporter of marine picocyanobacteria
Shin-Ichi Maeda, Akio Murakami, Hisashi Ito, Ayumi Tanaka, Tatsuo Omata
Life, 5, 1, 432, 446, MDPI AG, 2015年02月09日, ［査読有り］
英語, 研究論文（学術雑誌）

Accumulation of the NON-YELLOW COLORING 1 protein of the chlorophyll cycle requires chlorophyll b in Arabidopsis thaliana
Ting Jia, Hisashi Ito, Xueyun Hu, Ayumi Tanaka
PLANT JOURNAL, 81, 4, 586, 596, 2015年02月, ［査読有り］
英語, 研究論文（学術雑誌）

The Chlorophyll b Reductase NOL Participates in Regulating the Antenna Size of Photosystem II in Arabidopsis Thaliana.　　　　　　　　　　　　　　　
Jia Ting, 伊藤寿, 田中歩
Procedia Chemistry, 14, 422, 427, 2015年, ［査読有り］
英語, 研究論文（学術雑誌）

Evolution of a New Chlorophyll Metabolic Pathway Driven by the Dynamic Changes in Enzyme Promiscuous Activity
Hisashi Ito, Ayumi Tanaka
PLANT AND CELL PHYSIOLOGY, 55, 3, 593, 603, 2014年03月, ［査読有り］
英語, 研究論文（学術雑誌）

Protein co-migration database (PCoM -DB) for Arabidopsis thylakoids and Synechocystis cells
Atsushi Takabayashi, Ryosuke Kadoya, Masayoshi Kuwano, Katsunori Kurihara, Hisashi Ito, Ryouichi Tanaka, Ayumi Tanaka
SPRINGERPLUS, 2, 1, 148, 2013年, ［査読有り］
英語, 研究論文（学術雑誌）

Conversion of chlorophyll b to chlorophyll a precedes magnesium dechelation for protection against necrosis in Arabidopsis
Yousuke Shimoda, Hisashi Ito, Ayumi Tanaka
PLANT JOURNAL, 72, 3, 501, 511, 2012年11月, ［査読有り］
英語, 研究論文（学術雑誌）

Chlorophyll b Reductase Plays an Essential Role in Maturation and Storability of Arabidopsis Seeds
Saori Nakajima, Hisashi Ito, Ryouichi Tanaka, Ayumi Tanaka
PLANT PHYSIOLOGY, 160, 1, 261, 273, 2012年09月, ［査読有り］
英語, 研究論文（学術雑誌）

Luminescence of singlet oxygen in photosystem II complexes isolated from cyanobacterium Synechocystis sp. PCC6803 containing monovinyl or divinyl chlorophyll a.
Tomo T, Kusakabe H, Nagao R, Ito H, Tanaka A, Akimoto S, Mimuro M, Okazaki S
Biochimica et biophysica acta, 1817, 1299, 1305, 8, 2012年08月, ［査読有り］

Luminescence of singlet oxygen in photosystem II complexes isolated from cyanobacterium Synechocystis sp PCC6803 containing monovinyl or divinyl chlorophyll a
Tatsuya Tomo, Hayato Kusakabe, Ryo Nagao, Hisashi Ito, Ayumi Tanaka, Seiji Akimoto, Mamoru Mimuro, Shigetoshi Okazaki
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1817, 8, 1299, 1305, 2012年08月, ［査読有り］
英語, 研究論文（学術雑誌）

クロロフィル合成系の多様性はいかにして生まれたか? (解説特集 光合成を支えるテトラピロール代謝の多様性)
伊藤 寿, 田中 歩, 田中 亮一
光合成研究, 22, 2, 98, 105, 日本光合成学会, 2012年08月
日本語

珪藻のクロロフィル合成系の解析
伊藤寿, 田中歩
藻類, 60, 2, 117, 2012年07月10日
日本語

Alterations in photosynthetic pigments and amino acid composition of D1 protein change energy distribution in photosystem II.
Yokono M, Tomo T, Nagao R, Ito H, Tanaka A, Akimoto S
Biochimica et biophysica acta, 1817, 754, 759, 5, 2012年05月, ［査読有り］

Alterations in photosynthetic pigments and amino acid composition of D1 protein change energy distribution in photosystem II
Makio Yokono, Tatsuya Tomo, Ryo Nagao, Hisashi Ito, Ayumi Tanaka, Seiji Akimoto
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1817, 5, 754, 759, 2012年05月, ［査読有り］
英語, 研究論文（学術雑誌）

Chlorophyll Metabolism in Photosynthetic Organisms
Tanaka, R., Takabayashi, A., Ito, H., Tanaka, A.
Handbook of Porphyrin Science: With Applications to Chemistry, Physics, Materials Science, Engineering, Biology and Medicine: Volume 16 - 20: Synthetic Developments: Part I, 16-20, 2012年
研究論文（学術雑誌）

Evolution of a divinyl chlorophyll-based photosystem in Prochlorococcus
Hisashi Ito, Ayumi Tanaka
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108, 44, 18014, 18019, 2011年11月, ［査読有り］
英語, 研究論文（学術雑誌）

Identification of the 7-Hydroxymethyl Chlorophyll a Reductase of the Chlorophyll Cycle in Arabidopsis
Miki Meguro, Hisashi Ito, Atsushi Takabayashi, Ryouichi Tanaka, Ayumi Tanaka
PLANT CELL, 23, 9, 3442, 3453, 2011年09月, ［査読有り］
英語, 研究論文（学術雑誌）

Participation of Chlorophyll b Reductase in the Initial Step of the Degradation of Light-harvesting Chlorophyll a/b-Protein Complexes in Arabidopsis
Yukiko Horie, Hisashi Ito, Makoto Kusaba, Ryouichi Tanaka, Ayumi Tanaka
JOURNAL OF BIOLOGICAL CHEMISTRY, 284, 26, 17449, 17456, 2009年06月, ［査読有り］
英語, 研究論文（学術雑誌）

Replacement of chlorophyll with di-vinyl chlorophyll in the antenna and reaction center complexes of the cyanobacterium Synechocystis sp PCC 6803: Characterization of spectral and photochemical properties
Tatsuya Tomo, Seiji Akimoto, Hisashi Ito, Tohru Tsuchiya, Michitaka Fukuya, Ayumi Tanaka, Mamoru Mimuro
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1787, 3, 191, 200, 2009年03月, ［査読有り］
英語, 研究論文（学術雑誌）

Replacement of chlorophyll with di-vinyl chlorophyll in the antenna and reaction center complexes of the cyanobacterium Synechocystis sp. PCC 6803: characterization of spectral and photochemical properties.
Tomo T, Akimoto S, Ito H, Tsuchiya T, Fukuya M, Tanaka A, Mimuro M
Biochimica et biophysica acta, 1787, 3, 191, 200, 2009年03月, ［査読有り］

Identification of a novel vinyl reductase gene essential for the biosynthesis of monovinyl chlorophyll in Synechocystis sp PCC6803
Hisashi Ito, Makio Yokono, Ryouichi Tanaka, Ayumi Tanaka
JOURNAL OF BIOLOGICAL CHEMISTRY, 283, 14, 9002, 9011, 2008年04月, ［査読有り］
英語, 研究論文（学術雑誌）

Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence
Makoto Kusaba, Hisashi Ito, Ryouhei Morita, Shuichi Iida, Yutaka Sato, Masaru Fujimoto, Shinji Kawasaki, Ryouichi Tanaka, Hirohiko Hirochika, Minoru Nishimura, Ayumi Tanaka
PLANT CELL, 19, 4, 1362, 1375, 2007年04月, ［査読有り］
英語, 研究論文（学術雑誌）

High light inhibits chlorophyll biosynthesis at the level of 5-aminolevulinate synthesis during de-etiolation in cucumber (Cucumis sativus) cotyledons
D. Aarti, R. Tanaka, H. Ito, A. Tanaka
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 83, 1, 171, 176, 2007年01月, ［査読有り］
英語, 研究論文（学術雑誌）

海産藻類の光合成系と生産性の再評価 海洋性ラン藻ProchlorococcusとSynechococcusの光合成と進化
伊藤寿, 永田望, 田中歩
月刊海洋, 38, 6, 417, 424, 2006年06月01日
日本語

クロロフィルの生合成
田中 歩, 田中 亮一, 伊藤 寿, 蛋白質核酸酵素, 41, 2, 136, 145, 1996年02月
共立出版, 日本語