Sep. 2023, Japanese Society for Plant Biotechnology, The JSPB Excellent Paper Award　　　　　　　　　　　　　　　
3-Phynyllactic acid is converted to phenylacetic acid andinduces auxin-responsive root growth in Arabidopsis plants.
Yuko Maki, Hiroshi Soejima, Tamizi Sugiyama, Masaaki K. Watahiki, Takeo Sato, Junji Yamaguchi

Aug. 2018, Japanese Society for Plant Cell and Molecular Biology, Studies on environmental adaptation in response to nutrients in plants　　　　　　　　　　　　　　　
The JSPCMB Award for Distinguished Research
YAMAGUCHI Junji

Citrate pretreatment promotes rice (<i>Oryza sativa</i> L.) coleoptile elongation under submergence
Akio Kubo, Miho Sanagi, Yuko Maki, Ryosuke Koyari, Futoshi Sakuma, Junji Yamaguchi, Takeo Sato
Plant Biotechnology, 42, 1, 57, 64, Japanese Society for Plant Cell and Molecular Biology, 25 Mar. 2025
Scientific journal

Histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 proteins affect plant growth under nitrogen deficient conditions in Arabidopsis thaliana
Linnan Jie, Miho Sanagi, Yongming Luo, Haruna Maeda, Yoichiro Fukao, Yukako Chiba, Shuichi Yanagisawa, Junji Yamaguchi, Junpei Takagi, Takeo Sato
PLANT BIOTECHNOLOGY, 40, 1, 93, 98, Mar. 2023
English, Scientific journal

Deubiquitinating enzymes UBP12 and UBP13 regulate carbon/nitrogen-nutrient stress responses by interacting with the membrane-localized ubiquitin ligase ATL31 in Arabidopsis
Yongming Luo, Shigetaka Yasuda, Junpei Takagi, Yoko Hasegawa, Yukako Chiba, Junji Yamaguchi, Takeo Sato
Biochemical and Biophysical Research Communications, Elsevier BV, Oct. 2022
Scientific journal

Conjugates of 3-phenyllactic acid and tryptophan enhance root-promoting activity without adverse effects in Vigna angularis.
Yuko Maki, Hiroshi Soejima, Tamizi Sugiyama, Takeo Sato, Junji Yamaguchi, Masaaki K Watahiki
Plant biotechnology (Tokyo, Japan), 39, 2, 173, 177, 25 Jun. 2022, [Domestic magazines]
English, Scientific journal, 3-Phenyllactic acid (PLA) is a common secondary product of Lactobacillus sp. and promotes adventitious-root formation in Azuki beans (Vigna angularis). Root promotion activity of PLA is synergistically enhanced by tryptophan (Trp). In this study, stereoisomers of PLA and Trp amide conjugates and their alkyl esters were synthesized to investigate the structure-activity relationships on root-promotion activity. The rooting activity of D-PLA-L-Trp conjugate shows more than 40 times higher than that of the mixture of D-PLA and L-Trp. Modification of PLA-Trp with ethyl ester showed the highest activity at 3,400 times of a mixture of D-PLA and L-Trp. However, L-or D-PLA-D-Trp conjugate and the isopropyl ester of PLA-Trp conjugates, both lost the root promotion activity and implicated that a requirement for steric structure for PLA related root promotion mechanism. Unlike auxin substances, which are commonly used as rooting agents that displayed high activity in low concentrations, PLA-Trp ethyl ester exhibited far less phytotoxicity at high concentration of 1 mM, despite its high rooting activity. Innovation of PLA-Trp ethyl ester may be expected for agricultural aspects with low environmental impact.

3-Phenyllactic acid is converted to phenylacetic acid and induces auxin-responsive root growth in Arabidopsis plants.
Yuko Maki, Hiroshi Soejima, Tamizi Sugiyama, Masaaki K Watahiki, Takeo Sato, Junji Yamaguchi
Plant biotechnology (Tokyo, Japan), 39, 2, 111, 117, 25 Jun. 2022, [Domestic magazines]
English, Scientific journal, Many microorganisms have been reported to produce compounds that promote plant growth and are thought to be involved in the establishment and maintenance of symbiotic relationships. 3-Phenyllactic acid (PLA) produced by lactic acid bacteria was previously shown to promote root growth in adzuki cuttings. However, the mode of action of PLA as a root-promoting substance had not been clarified. The present study therefore investigated the relationship between PLA and auxin. PLA was found to inhibit primary root elongation and to increase lateral root density in wild-type Arabidopsis, but not in an auxin signaling mutant. In addition, PLA induced IAA19 promoter fused β-glucuronidase gene expression, suggesting that PLA exhibits auxin-like activity. The inability of PLA to promote degradation of Auxin/Indole-3-Acetic Acid protein in a yeast heterologous reconstitution system indicated that PLA may not a ligand of auxin receptor. Using of a synthetic PLA labeled with stable isotope showed that exogenously applied PLA was converted to phenylacetic acid (PAA), an endogenous auxin, in both adzuki and Arabidopsis. Taken together, these results suggest that exogenous PLA promotes auxin signaling by conversion to PAA, thereby regulating root growth in plants.

Deubiquitinating enzymes UBP12 and UBP13 stabilize the brassinosteroid receptor BRI1.
Yongming Luo, Junpei Takagi, Lucas Alves Neubus Claus, Chao Zhang, Shigetaka Yasuda, Yoko Hasegawa, Junji Yamaguchi, Libo Shan, Eugenia Russinova, Takeo Sato
EMBO reports, 23, 4, e53354, 05 Apr. 2022, [International Magazine]
English, Scientific journal, Protein ubiquitination is a dynamic and reversible post-translational modification that controls diverse cellular processes in eukaryotes. Ubiquitin-dependent internalization, recycling, and degradation are important mechanisms that regulate the activity and the abundance of plasma membrane (PM)-localized proteins. In plants, although several ubiquitin ligases are implicated in these processes, no deubiquitinating enzymes (DUBs), have been identified that directly remove ubiquitin from membrane proteins and limit their vacuolar degradation. Here, we discover two DUB proteins, UBP12 and UBP13, that directly target the PM-localized brassinosteroid (BR) receptor BR INSENSITIVE1 (BRI1) in Arabidopsis. BRI1 protein abundance is decreased in the ubp12i/ubp13 double mutant that displayed severe growth defects and reduced sensitivity to BRs. UBP13 directly interacts with and effectively removes K63-linked polyubiquitin chains from BRI1, thereby negatively modulating its vacuolar targeting and degradation. Our study reveals that UBP12 and UBP13 play crucial roles in governing BRI1 abundance and BR signaling activity to regulate plant growth.

The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis.
Yoko Hasegawa, Thais Huarancca Reyes, Tomohiro Uemura, Anirban Baral, Akari Fujimaki, Yongming Luo, Yoshie Morita, Yasushi Saeki, Shugo Maekawa, Shigetaka Yasuda, Koki Mukuta, Yoichiro Fukao, Keiji Tanaka, Akihiko Nakano, Junpei Takagi, Rishikesh P Bhalerao, Junji Yamaguchi, Takeo Sato
The Plant cell, 34, 4, 1354, 1374, 29 Mar. 2022, [International Magazine]
English, Scientific journal, Ubiquitination is a post-translational modification involving the reversible attachment of the small protein ubiquitin to a target protein. Ubiquitination is involved in numerous cellular processes, including the membrane trafficking of cargo proteins. However, the ubiquitination of the trafficking machinery components and their involvement in environmental responses are not well understood. Here, we report that the Arabidopsis thaliana trans-Golgi network/early endosome localized SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein SYP61 interacts with the transmembrane ubiquitin ligase ATL31, a key regulator of resistance to disrupted carbon (C)/nitrogen/(N)-nutrient conditions. SYP61 is a key component of membrane trafficking in Arabidopsis. The subcellular localization of ATL31 was disrupted in knockdown mutants of SYP61, and the insensitivity of ATL31-overexpressing plants to high C/low N-stress was repressed in these mutants, suggesting that SYP61 and ATL31 cooperatively function in plant responses to nutrient stress. SYP61 is ubiquitinated in plants, and its ubiquitination level is upregulated under low C/high N-nutrient conditions. These findings provide important insights into the ubiquitin signaling and membrane trafficking machinery in plants.

Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis.
Miho Sanagi, Shoki Aoyama, Akio Kubo, Yu Lu, Yasutake Sato, Shogo Ito, Mitsutomo Abe, Nobutaka Mitsuda, Masaru Ohme-Takagi, Takatoshi Kiba, Hirofumi Nakagami, Filip Rolland, Junji Yamaguchi, Takato Imaizumi, Takeo Sato
Proceedings of the National Academy of Sciences of the United States of America, 118, 19, 11 May 2021, [International Magazine]
English, Scientific journal, Nitrogen (N) is an essential nutrient that affects multiple plant developmental processes, including flowering. As flowering requires resources to develop sink tissues for reproduction, nutrient availability is tightly linked to this process. Low N levels accelerate floral transition; however, the molecular mechanisms underlying this response are not well understood. Here, we identify the FLOWERING BHLH 4 (FBH4) transcription factor as a key regulator of N-responsive flowering in Arabidopsis Low N-induced early flowering is compromised in fbh quadruple mutants. We found that FBH4 is a highly phosphorylated protein and that FBH4 phosphorylation levels decrease under low N conditions. In addition, decreased phosphorylation promotes FBH4 nuclear localization and transcriptional activation of the direct target CONSTANS (CO) and downstream florigen FLOWERING LOCUS T (FT) genes. Moreover, we demonstrate that the evolutionarily conserved cellular fuel sensor SNF1-RELATED KINASE 1 (SnRK1), whose kinase activity is down-regulated under low N conditions, directly phosphorylates FBH4. SnRK1 negatively regulates CO and FT transcript levels under high N conditions. Together, these results reveal a mechanism by which N levels may fine-tune FBH4 nuclear localization by adjusting the phosphorylation state to modulate flowering time. In addition to its role in flowering regulation, we also showed that FBH4 was involved in low N-induced up-regulation of nutrient recycling and remobilization-related gene expression. Thus, our findings provide insight into N-responsive growth phase transitions and optimization of plant fitness under nutrient-limited conditions.

3-Phenyllactic acid, a root-promoting substance isolated from Bokashi fertilizer, exhibits synergistic effects with tryptophan.
Yuko Maki, Hiroshi Soejima, Toru Kitamura, Tamizi Sugiyama, Takeo Sato, Masaaki K Watahiki, Junji Yamaguchi
Plant biotechnology (Tokyo, Japan), 38, 1, 9, 16, 25 Mar. 2021, [Domestic magazines]
English, Scientific journal, Bokashi fertilizer, an organic fertilizer made of plant residue, has been used in Japan not only to fertilize plants but to regulate their growth. Lactic acid bacteria have been found to play an important role in the fermentation process of Bokashi, but the relationship between these bacteria and plant growth activity has not been clarified. Using the adzuki rooting assay, this study identified 3-phenyllactic acid (PLA) produced by lactic acid bacteria as a root promoting compound in Bokashi. PLA showed synergistic effect with tryptophan, but no stem elongation activity. Lactic acid bacteria produced equal quantities of the L- and D-forms of PLA, which have similar root promoting activity. PLA did not significantly affect the amount of endogenous indole-3-acetic acid (IAA), although the chemical structure of PLA is highly similar to that of L-2-aminooxy-3-phenypropionic acid (L-AOPP), which inhibits IAA biosynthesis. These results indicate that the root promoting activity of PLA is not simply due to its increase in the amount of active auxin.

Effect of circularly polarized light on germination, hypocotyl elongation and biomass production of arabidopsis and lettuce: Involvement of phytochrome B.
Enkhsukh Lkhamkhuu, Kazunori Zikihara, Hitomi Katsura, Satoru Tokutomi, Takafumi Hosokawa, Yoshihisa Usami, Mitsuyoshi Ichihashi, Junji Yamaguchi, Kenji Monde
Plant biotechnology (Tokyo, Japan), 37, 1, 57, 67, Japanese Society for Plant Cell and Molecular Biology, 25 Mar. 2020, [Domestic magazines]
English, Scientific journal, Circular dichroism (CD), defined as the differential absorption of left- and right-handed circularly polarized light (CPL), is a useful spectroscopic technique for structural studies of biological systems composed of chiral molecules. The present study evaluated the effects of CPL on germination, hypocotyl elongation and biomass production of Arabidopsis and lettuce. Higher germination rates were observed when Arabidopsis and lettuce seedlings were irradiated with red right-handed CPL (R-CPL) than with red left-handed CPL (L-CPL). Hypocotyl elongation was effectively inhibited when Arabidopsis and lettuce seedlings were irradiated with red R-CPL than with red L-CPL. This difference was not observed when a phytochrome B (phyB) deficient mutant of Arabidopsis was irradiated, suggesting that inhibition of elongation by red R-CPL was mediated by phyB. White R-CPL induced greater biomass production by adult Arabidopsis plants, as determined by their fresh shoot weight, than white L-CPL. To determine the molecular basis of these CPL effects, CD spectra and the effect of CPL on the photoreaction of a sensory module of Arabidopsis phyB were measured. The red light-absorbing form of phyB showed a negative CD in the red light-absorbing region, consistent with the results of germination, inhibition of hypocotyl elongation and biomass production. L-CPL and R-CPL, however, did not differ in their ability to induce the interconversion of the red light-absorbing and far-red light-absorbing forms of phyB. These findings suggest that these CPL effects involve phyB, along with other photoreceptors and the photosynthetic process.

Proteome Analysis of 14-3-3 Targets in Tomato Fruit Tissues.
Yongming Luo, Yu Lu, Junji Yamaguchi, Takeo Sato
Methods in molecular biology (Clifton, N.J.), 2139, 289, 296, 2020, [International Magazine]
English, Scientific journal, Tomato is a major crop plant and an important constituent of the human diet. Exclusive features such as bearing fleshy fruits and undergoing a phase transition from partially photosynthetic to fully heterotrophic metabolism make tomato fruit a model system for fruit development studies. Although the tomato genome has been completely sequenced, functional proteomics studies are still at their starting stage. Proteomics technologies, especially the combination of multiple approaches, provide a very powerful tool to accurately identify functional proteins and investigate certain sets of proteins in more detail. The direct binding of plant 14-3-3 proteins to their multiple target proteins modulates the functions of the latter, suggesting that these 14-3-3 proteins are directly involved in various physiological pathways. This chapter outline methods for the identification of 14-3-3 protein complexes in tomato fruit tissues. These methods include detailed protocols for protein extraction, coimmunoprecipitation, SDS-PAGE, SYPRO Ruby staining, in-gel trypsin digestion, and LC-MS/MS analysis for 14-3-3 interactomics.

Protein Phosphorylation Dynamics Under Carbon/Nitrogen-Nutrient Stress and Identification of a Cell Death-Related Receptor-Like Kinase in Arabidopsis.
Xingwen Li, Miho Sanagi, Yu Lu, Yuko Nomura, Sara Christina Stolze, Shigetaka Yasuda, Yusuke Saijo, Waltraud X Schulze, Regina Feil, Mark Stitt, John E Lunn, Hirofumi Nakagami, Takeo Sato, Junji Yamaguchi
Frontiers in plant science, 11, 377, 377, 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Nutrient availability, in particular the availability of sugar [carbon (C)] and nitrogen (N), is important for the regulation of plant metabolism and development. In addition to independent utilization of C and N nutrients, plants sense and respond to the balance of C and N nutrients (C/N-nutrient) available to them. High C/low N-nutrient stress has been shown to arrest early post-germinative growth while promoting progression to senescence in Arabidopsis. Although several signaling components of the C/N-nutrient response have been identified, the inclusive molecular basis of plant C/N-nutrient response remains unclear. This proteome analysis evaluated phosphorylation dynamics in response to high C/low N-nutrient stress. Phosphoproteomics under conditions of C/N-nutrient stress showed a global change in the phosphorylation status of proteins, including plasma membrane H+-ATPase, carbon and nitrogen metabolic enzymes and signaling proteins such as protein kinases and transcription factors. Further analyses suggested that SNF1-related protein kinase 1 (SnRK1) is involved in primary C/N-nutrient signal mediation via the transcriptional regulation of C/N-regulatory kinases. We also identified a leucine-rich repeat receptor-like kinase with extracellular malectin-like domain, named as LMK1, which was shown to possess cell death induction activity in plant leaves. These results provide important insight into the C/N-nutrient signaling pathways connecting nutrition stress to various cellular and physiological processes in plants.

Identification of Arabidopsis CCR4-NOT Complexes with Pumilio RNA-Binding Proteins, APUM5 and APUM2.
Toshihiro Arae, Kotone Morita, Riko Imahori, Yuya Suzuki, Shigetaka Yasuda, Takeo Sato, Junji Yamaguchi, Yukako Chiba
Plant & cell physiology, 60, 9, 2015, 2025, 01 Sep. 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, CCR4/CAF1 are widely conserved deadenylases in eukaryotes. They form a large complex that includes NOT1 as a scaffold protein and various NOT proteins that are core components of multiple levels of gene expression control. The CCR4-NOT complex also contains several RNA-binding proteins as accessory proteins, which are required for target recognition by CCR4/CAF1 deadenylases. AtCCR4a/b, orthologs of human CCR4 in Arabidopsis, have various physiological effects. AtCCR4 isoforms are likely to have specific target mRNAs related to each physiological effect; however, AtCCR4 does not have RNA-binding capability. Therefore, identifying factors that interact with AtCCR4a/b is indispensable to understand its function as a regulator of gene expression, as well as the target mRNA recognition mechanism. Here, we identified putative components of the AtCCR4-NOT complex using co-immunoprecipitation in combination with mass spectrometry using FLAG-tagged AtCCR4b and subsequent verification with a yeast two-hybrid assay. Interestingly, four of 11 AtCAF1 isoforms interacted with both AtCCR4b and AtNOT1, whereas two isoforms interacted only with AtNOT1 in yeast two-hybrid assays. These results imply that Arabidopsis has multiple CCR4-NOT complexes with various combinations of deadenylases. We also revealed that the RNA-binding protein Arabidopsis Pumilio 5 and 2 interacted with AtCCR4a/b in the cytoplasm with a few foci.

Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis.
Yongming Luo, Shoki Aoyama, Yoichiro Fukao, Yukako Chiba, Takeo Sato, Junji Yamaguchi
Plant biotechnology (Tokyo, Japan), 36, 2, 107, 112, Japanese Society for Plant Cell and Molecular Biology, 25 Jun. 2019, [Domestic magazines]
English, Scientific journal, As major components of the ubiquitin system, ubiquitin ligases mediate the transfer of ubiquitin to specific target substrates, thereby playing important roles in regulating a wide range of cellular processes. The Arabidopsis Tóxicos en Levadura (ATL) family is a group of plant-specific RING-type ubiquitin ligases with N-terminal transmembrane-like domains. To date, 91 ATL isoforms have been identified in the Arabidopsis genome, with some reported to regulate plant responses to environmental stresses. However, the functions of most ATLs remain unclear. This study showed that ATL8 is a sugar starvation response gene and that ATL8 expression was significantly increased by sugar starvation conditions but repressed by exogenous sugar supply. The ATL8 protein was found to possess ubiquitin ligase activity in vitro and to localize to membrane-bound compartments in plant cells. In addition, Starch Synthase 4 was identified as a putative interactor with ATL8, suggesting that ATL8 may be involved in modulating starch accumulation in response to sugar availability. These findings suggest that ATL8 functions as a membrane-localized ubiquitin ligase likely to be involved in the adaptation of Arabidopsis plants to sugar starvation stress.

Sugar-responsive transcription factor bZIP3 affects leaf shape in Arabidopsis plants.
Miho Sanagi, Yu Lu, Shoki Aoyama, Yoshie Morita, Nobutaka Mitsuda, Miho Ikeda, Masaru Ohme-Takagi, Takeo Sato, Junji Yamaguchi
Plant biotechnology (Tokyo, Japan), 35, 2, 167, 170, Japanese Society for Plant Cell and Molecular Biology, 25 Jun. 2018, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Sugars are essential for plant metabolism, growth and development. Plants must therefore manage their growth and developmental processes in response to sugar availability. Sugar signaling pathways constitute a complicated molecular network and are associated with global transcriptional regulation. However, the molecular mechanisms underlying sugar signaling remain largely unclear. This study reports that the protein basic-region leucine zipper 3 (bZIP3) is a novel sugar-responsive transcription factor in Arabidopsis plants. The expression of bZIP3 was rapidly repressed by sugar. Genetic analysis indicated that bZIP3 expression was modulated by the SNF1-RELATED KINASE 1 (SnRK1) pathway. Moreover, transgenic plants overexpressing bZIP3 and dominant repressor form bZIP3-SRDX showed aberrant shaped cotyledons with hyponastic bending. These findings suggest that bZIP3 plays a role in plant responses to sugars and is also associated with leaf development.

Nitrate Reductase Modulation in Response to Changes in C/N Balance and Nitrogen Source in Arabidopsis.
Thais Huarancca Reyes, Andrea Scartazza, Antonio Pompeiano, Andrea Ciurli, Yu Lu, Lorenzo Guglielminetti, Junji Yamaguchi
Plant & cell physiology, 59, 6, 1248, 1254, Oxford University Press, 01 Jun. 2018, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Membrane-localized ubiquitin ligase ATL15 functions in sugar-responsive growth regulation in Arabidopsis
Shoki Aoyama, Saki Terada, Miho Sanagi, Yoko Hasegawa, Yu Lu, Yoshie Morita, Yukako Chiba, Takeo Sato, Junji Yamaguchi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 491, 1, 33, 39, Sep. 2017, [Peer-reviewed]
English, Scientific journal

Co-ordinated Regulations of mRNA Synthesis and Decay during Cold Acclimation in Arabidopsis Cells
Toshihiro Arae, Shiori Isai, Akira Sakai, Katsuhiko Mineta, Masami Yokota Hirai, Yuya Suzuki, Shigehiko Kanaya, Junji Yamaguchi, Satoshi Naito, Yukako Chiba
PLANT AND CELL PHYSIOLOGY, 58, 6, 1090, 1102, Jun. 2017, [Peer-reviewed]
English, Scientific journal

Arabidopsis CBL-Interacting Protein Kinases Regulate Carbon/Nitrogen-Nutrient Response by Phosphorylating Ubiquitin Ligase ATL31
Shigetaka Yasuda, Shoki Aoyama, Yoko Hasegawa, Takeo Sato, Junji Yamaguchi
MOLECULAR PLANT, 10, 4, 605, 618, Apr. 2017, [Peer-reviewed]
English, Scientific journal

Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis
Takeo Sato, Shugo Maekawa, Mineko Konishi, Nozomi Yoshioka, Yuki Sasaki, Haruna Maeda, Tetsuya Ishida, Yuki Kato, Junji Yamaguchi, Shuichi Yanagisawa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 483, 1, 380, 386, Jan. 2017, [Peer-reviewed]
English, Scientific journal

Effect of carbon/nitrogen ratio on carbohydrate metabolism and light energy dissipation mechanisms in Arabidopsis thaliana
Thais Huarancca Reyes, Andrea Scartazza, Yu Lu, Junji Yamaguchi, Lorenzo Guglielminetti
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 105, 195, 202, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Characterization of ubiquitin ligase SlATL31 and proteomic analysis of 14-3-3 targets in tomato fruit tissue (Solanum lycopersicum L.)
Yu Lu, Shigetaka Yasuda, Xingwen Li, Yoichiro Fukao, Takayuki Tohge, Alisdair R. Fernie, Chiaki Matsukura, Hiroshi Ezura, Takeo Sato, Junji Yamaguchi
JOURNAL OF PROTEOMICS, 143, 254, 264, Jun. 2016, [Peer-reviewed]
English, Scientific journal

P4-1-15 硝酸シグナル応答型転写因子NLPはBT群の遺伝子発現制御を介して側根伸長を制御する（ポスター，4-1 植物の多量栄養素，2016年度佐賀大会）
前川 修吾, 吉岡 希, 小西 美稲子, 石田 哲也, 加藤 祐樹, 佐々木 勇樹, 佐藤 長緒, 山口 淳二, 柳澤 修一
日本土壌肥料学会講演要旨集, 62, 55, 55, 一般社団法人 日本土壌肥料学会, 2016
Japanese

Integration of C/N-nutrient and multiple environmental signals into the ABA signaling cascade
Yu Lu, Junji Yamaguchi, Takeo Sato
PLANT SIGNALING & BEHAVIOR, 10, 12, e1048940, Dec. 2015, [Peer-reviewed]
English

Erratum: ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis (Journal of Experimental Botany (2015) 66:9 (2763-2771))
Yu Lu, Yuki Sasaki, Xingwen Li, Izumi C. Mori, Takakazu Matsuura, Takashi Hirayama, Takeo Sato, Junji Yamaguchi
Journal of Experimental Botany, 66, 15, 4851, Oxford University Press, 01 Aug. 2015, [Peer-reviewed]
English, Scientific journal

ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis (vol 66, pg 2763, 2015)
Yu Lu, Yuki Sasaki, Xingwen Li, Izumi C. Mori, Takakazu Matsuura, Takashi Hirayama, Takeo Sato, Junji Yamaguchi
JOURNAL OF EXPERIMENTAL BOTANY, 66, 15, 4851, 4851, Aug. 2015, [Peer-reviewed]
English

AtCCR4a and AtCCR4b are Involved in Determining the Poly(A) Length of Granule-bound starch synthase 1 Transcript and Modulating Sucrose and Starch Metabolism in Arabidopsis thaliana
Yuya Suzuki, Toshihiro Arae, Pamela J. Green, Junji Yamaguchi, Yukako Chiba
PLANT AND CELL PHYSIOLOGY, 56, 5, 863, 874, May 2015, [Peer-reviewed]
English, Scientific journal

ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis
Yu Lu, Yuki Sasaki, Xingwen Li, Izumi C. Mori, Takakazu Matsuura, Takashi Hirayama, Takeo Sato, Junji Yamaguchi
JOURNAL OF EXPERIMENTAL BOTANY, 66, 9, 2763, 2771, May 2015, [Peer-reviewed]
English, Scientific journal

The Arabidopsis ubiquitin ligase ATL31 is transcriptionally controlled by WRKY33 transcription factor in response to pathogen attack
Thais Huarancca Reyes, Shugo Maekawa, Takeo Sato, Junji Yamaguchi
PLANT BIOTECHNOLOGY, 32, 1, 11, +, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Pale-Green Phenotype of atl31 atl6 Double Mutant Leaves Is Caused by Disruption of 5-Aminolevulinic Acid Biosynthesis in Arabidopsis thaliana
Shugo Maekawa, Atsushi Takabayashi, Thais Huarancca Reyes, Hiroko Yamamoto, Ayumi Tanaka, Takeo Sato, Junji Yamaguchi
PLOS ONE, 10, 2, e0117662, Feb. 2015, [Peer-reviewed]
English, Scientific journal

Exploration of substrates for a membrane-localized ubiquitin ligase in plant innate immunity
Yasuda Shigetaka, Hasegawa Yoko, Kadota Yasuhiro, Fukao Yoichiro, Sato Takeo, Yamaguchi Junji
Abstracts for Annual Meeting of Japanese Proteomics Society, 2015, 232, 232, Japanese Proteomics Society (Japan Human Proteome Organisation), 2015
Japanese

Proteomic Analysis of the 26S Proteasome Reveals Its Direct Interaction with Transit Peptides of Plastid Protein Precursors for Their Degradation
Kaori Sako, Yuki Yanagawa, Tomoyuki Kanai, Takeo Sato, Motoaki Seki, Masayuki Fujiwara, Yoichiro Fukao, Junji Yamaguchi
JOURNAL OF PROTEOME RESEARCH, 13, 7, 3223, 3230, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Phosphorylation of Arabidopsis Ubiquitin Ligase ATL31 Is Critical for Plant Carbon/Nitrogen Nutrient Balance Response and Controls the Stability of 14-3-3 Proteins
Shigetaka Yasuda, Takeo Sato, Shugo Maekawa, Shoki Aoyama, Yoichiro Fukao, Junji Yamaguchi
JOURNAL OF BIOLOGICAL CHEMISTRY, 289, 22, 15179, 15193, May 2014, [Peer-reviewed]
English, Scientific journal

Regulation of senescence under elevated atmospheric CO2 via ubiquitin modification
Shoki Aoyama, Yu Lu, Junji Yamaguchi, Takeo Sato
Plant Signaling and Behavior, 9, 5, e28839, Landes Bioscience, 16 Apr. 2014, [Peer-reviewed]
English

The Carbon/Nitrogen Regulator ARABIDOPSIS TOXICOS EN LEVADURA31 Controls Papilla Formation in Response to Powdery Mildew Fungi Penetration by Interacting with SYNTAXIN OF PLANTS121 in Arabidopsis
Shugo Maekawa, Noriko Inada, Shigetaka Yasuda, Yoichiro Fukao, Masayuki Fujiwara, Takeo Sato, Junji Yamaguchi
PLANT PHYSIOLOGY, 164, 2, 879, 887, Feb. 2014, [Peer-reviewed]
English, Scientific journal

Ubiquitin Ligase ATL31 Functions in Leaf Senescence in Response to the Balance Between Atmospheric CO2 and Nitrogen Availability in Arabidopsis
Shoki Aoyama, Thais Huarancca Reyes, Lorenzo Guglielminetti, Yu Lu, Yoshie Morita, Takeo Sato, Junji Yamaguchi
PLANT AND CELL PHYSIOLOGY, 55, 2, 293, 305, Feb. 2014, [Peer-reviewed]
English, Scientific journal

The Arabidopsis transcriptional repressor ERF9 participates in resistance against necrotrophic fungi
Yosuke Maruyama, Natsuko Yamoto, Yuya Suzuki, Yukako Chiba, Ken-ichi Yamazaki, Takeo Sato, Junji Yamaguchi
PLANT SCIENCE, 213, 79, 87, Dec. 2013, [Peer-reviewed]
English, Scientific journal

Proteomics Analysis Reveals a Highly Heterogeneous Proteasome Composition and the Post-translational Regulation of Peptidase Activity under Pathogen Signaling in Plants
Hui H. Sun, Yoichiro Fukao, Sakiko Ishida, Hiroko Yamamoto, Shugo Maekawa, Masayuki Fujiwara, Takeo Sato, Junji Yamaguchi
JOURNAL OF PROTEOME RESEARCH, 12, 11, 5084, 5095, Nov. 2013, [Peer-reviewed]
English, Scientific journal

Changes in mRNA stability associated with cold stress in arabidopsis cells
Yukako Chiba, Katsuhiko Mineta, Masami Y. Hirai, Yuya Suzuki, Shigehiko Kanaya, Hiro Takahashi, Hitoshi Onouchi, Junji Yamaguchi, Satoshi Naito
Plant and Cell Physiology, 54, 2, 181, 194, Oxford University Press, 2013, [Peer-reviewed]
English, Scientific journal

The Arabidopsis ubiquitin ligases ATL31 and ATL6 control the defense response as well as the carbon/nitrogen response
Shugo Maekawa, Takeo Sato, Yutaka Asada, Shigetaka Yasuda, Midori Yoshida, Yukako Chiba, Junji Yamaguchi
PLANT MOLECULAR BIOLOGY, 79, 3, 217, 227, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Arabidopsis RPT2a, 19S Proteasome Subunit, Regulates Gene Silencing via DNA Methylation
Kaori Sako, Yuko Maki, Tomoyuki Kanai, Eriko Kato, Shugo Maekawa, Shigetaka Yasuda, Takeo Sato, Masaaki K. Watahiki, Junji Yamaguchi
PLOS ONE, 7, 5, e37086, May 2012, [Peer-reviewed]
English, Scientific journal

Proteomics approach to study metabolic regulation mediated by ubiquitin-proteasome system in Arabidopsis　　　　　　　　　　　　　　　
Yasuda S, Maekawa S, Sato T, Yamaguchi J
Frontiers of Agriculture Proteome Research, 39, 44, 2012, [Peer-reviewed]

Sugar-inducible RPT2a, a subunit of 26S proteasome, participates in sugar response in Arabidopsis
Sun Huihui, Sako Kaori, Suzuki Yuya, Maekawa Shugo, Yasuda Shigetaka, Chiba Yukako, Sato Takeo, Yamaguchi Junji
PLANT BIOTECHNOLOGY, 29, 3, 279, 284, 2012, [Peer-reviewed]
English

Identification of 14-3-3 proteins as a target of ATL31 ubiquitin ligase, a regulator of the C/N response in Arabidopsis
Takeo Sato, Shugo Maekawa, Shigetaka Yasuda, Yukie Domeki, Kuni Sueyoshi, Masayuki Fujiwara, Yoichiro Fukao, Derek B. Goto, Junji Yamaguchi
PLANT JOURNAL, 68, 1, 137, 146, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture
Emiko Okubo-Kurihara, Takumi Higaki, Yukio Kurihara, Natsumaro Kutsuna, Junji Yamaguchi, Seiichiro Hasezawa
JOURNAL OF PLANT RESEARCH, 124, 3, 395, 403, May 2011
English, Scientific journal

Arabidopsis thaliana 26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance
Takuya Sakamoto, Takehiro Kamiya, Kaori Sako, Junji Yamaguchi, Mutsumi Yamagami, Toru Fujiwara
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75, 3, 561, 567, Mar. 2011, [Peer-reviewed]
English, Scientific journal

The Arabidopsis NSL2 negatively controls systemic acquired resistance via hypersensitive response
Yutaka Asada, Masako Yamamoto, Tomokazu Tsutsui, Junji Yamaguchi
PLANT BIOTECHNOLOGY, 28, 1, 9, 15, 2011
English, Scientific journal

Carbon and nitrogen metabolism regulated by ubiquitin-proteasome system.
Plant and Signaling Behavior, 6, 1465, 1468, 2011

Carbon and nitrogen metabolism regulated by the ubiquitin-proteasome system
Takeo Sato, Shugo Maekawa, Shigetaka Yasuda, Junji Yamaguchi
Plant Signaling and Behavior, 6, 10, 1465, 1468, Landes Bioscience, 2011, [Peer-reviewed]
English, Scientific journal

Studies on Plant Ubiquitin-Proteasome System by Proteomics Approaches—our Case Study on Metabolic Regulation—
Yamaguchi Junji, Satoh Takeo
Abstracts for Annual Meeting of Japanese Proteomics Society, 2011, 64, 64, Japanese Proteomics Society (Japan Human Proteome Organisation), 2011
Japanese

Control of endoreduplication of trichome by RPT2a, a subunit of the 19S proteasome in Arabidopsis
Kaori Sako, Yuko Maki, Kumiko K. Imai, Takashi Aoyama, Derek B. Goto, Junji Yamaguchi
JOURNAL OF PLANT RESEARCH, 123, 5, 701, 706, Sep. 2010
English, Scientific journal

How does the plant proteasome control leaf size?
Kaori Sako, Junji Yamaguchi
Plant Signaling and Behavior, 5, 9, 1119, 1120, 9, Sep. 2010, [Peer-reviewed]
English, Scientific journal

In planta observation of live fluorescent plant endoparasitic nematodes during early stages of infection
佐久間 太, 植原 健人, 近藤 則夫, 山口 淳二
Nematological research, 40, 1, 15, 19, 日本線虫学会, 2010
English, 内部寄生性植物線虫の感染初期の研究は重要であるが、寄主植物根内の線虫を生きたまま観察できないことが障害となっていた。そこで、二酢酸フルオレセイン（FDA）の0.001％溶液を用い、生きたネコブセンチュウとネグサレセンチュウを蛍光標識して、非破壊的な蛍光観察を行った。その結果、FDAで処理したネコブセンチュウは生物活性を保っており、寄主のトマト根内で感染場所が検出できることが示された。実験系として用いたシロイヌナズナでは、寄主植物根の中に侵入した線虫を落射蛍光顕微鏡下で直接観察することができ、FDA由来の蛍光が処理後少なくとも4日間検出された。FDAで処理したネグサレセンチュウも容易に寄主植物に感染したが、ネグサレセンチユウは検出可能な自己蛍光を示したため、非破壊的な検出のためのFDA処理は必要ないことが分かった。この報告は、線虫感染の初期段階において、高度な時空間分析に基づく非破壊的解析が可能であることを実証した。

CNI1/ATL31, a RING-type ubiquitin ligase that functions in the carbon/nitrogen response for growth phase transition in Arabidopsis seedlings.
Sato T, Maekawa S, Yasuda S, Sonoda Y, Katoh E, Ichikawa T, Nakazawa M, Seki M, Shinozaki K, Matsui M, Goto DB, Ikeda A, Yamaguchi J
The Plant journal : for cell and molecular biology, 60, 5, 852, 864, Dec. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal, Plants are able to sense and respond to changes in the balance between carbon (C) and nitrogen (N) metabolite availability, known as the C/N response. During the transition to photoautotrophic growth following germination, growth of seedlings is arrested if a high external C/N ratio is detected. To clarify the mechanisms for C/N sensing and signaling during this transition period, we screened a large collection of FOX transgenic plants, overexpressing full-length cDNAs, for individuals able to continue post-germinative growth under severe C/N stress. One line, cni1-D (carbon/nitrogen insensitive 1-dominant), was shown to have a suppressed sensitivity to C/N conditions at both the physiological and molecular level. The CNI1 cDNA encoded a predicted RING-type ubiquitin ligase previously annotated as ATL31. Overexpression of ATL31 was confirmed to be responsible for the cni1-D phenotype, and a knock-out of this gene resulted in hypersensitivity to C/N conditions during post-germinative growth. The ATL31 protein was confirmed to contain ubiquitin ligase activity using an in vitro assay system. Moreover, removal of this ubiquitin ligase activity from the overexpressed protein resulted in the loss of the mutant phenotype. Taken together, these data demonstrated that CNI1/ATL31 activity is required for the plant C/N response during seedling growth transition.

DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis
Tomokazu Tsutsui, Wataru Kato, Yutaka Asada, Kaori Sako, Takeo Sato, Yutaka Sonoda, Satoshi Kidokoro, Kazuko Yamaguchi-Shinozaki, Masanori Tamaoki, Keita Arakawa, Takanari Ichikawa, Miki Nakazawa, Motoaki Seki, Kazuo Shinozaki, Minami Matsui, Akira Ikeda, Junji Yamaguchi
JOURNAL OF PLANT RESEARCH, 122, 6, 633, 643, Nov. 2009
English, Scientific journal

Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Yutaka Sonoda, Kaori Sako, Yuko Maki, Naoko Yamazaki, Hiroko Yamamoto, Akira Ikeda, Junji Yamaguchi
PLANT JOURNAL, 60, 1, 68, 78, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Arabidopsis MBF1s Control Leaf Cell Cycle and its Expansion
Takuto Tojo, Kenichi Tsuda, Takeshi Yoshizumi, Akira Ikeda, Junji Yamaguchi, Minami Matsui, Ken-ichi Yamazaki
PLANT AND CELL PHYSIOLOGY, 50, 2, 254, 264, Feb. 2009
English, Scientific journal

Arabidopsis CAD1 negatively controls plant immunity mediated by both salicylic acid-dependent and -independent signaling pathways
Tomokazu Tsutsui, Yutaka Asada, Masanori Tamaoki, Akira Ikeda, Junji Yamaguchi
PLANT SCIENCE, 175, 4, 604, 611, Oct. 2008
English, Scientific journal

Promoter analysis of OsAMT1;2 and OsAMT1;3 implies their distinct roles in nitrogen utilization in rice
Shan-Guo Yao, Yutaka Sonoda, Tomokazu Tsutsui, Hidemitsu Nakamura, Hiroaki Ichikawa, Akira Ikeda, Junji Yamaguchi
BREEDING SCIENCE, 58, 3, 201, 207, Sep. 2008
English, Scientific journal

alpha-amylase production is induced by sulfuric acid in rice aleurone cells
Shin-ichiro Mitsunaga, Midori Kobayashi, Satoe Fukui, Kayoko Fukuoka, Osamu Kawakami, Junji Yamaguchi, Masahiro Ohshima, Toshiaki Mitsui
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 45, 12, 922, 925, Dec. 2007
English, Scientific journal

SHA1, a novel RING finger protein, functions in shoot apical meristem maintenance in Arabidopsis
Yutaka Sonoda, Shan-Guo Yao, Kaori Sako, Takeo Sato, Wataru Kato, Masa-aki Ohto, Takanari Ichikawa, Minami Matsui, Junji Yamaguchi, Akira Ikeda
PLANT JOURNAL, 50, 4, 586, 596, May 2007, [Peer-reviewed]
English, Scientific journal

Isolation and characterization of sugar insensitive mutant in Arabidopsis
Takeo Sato, Wataru Kato, Yutaka Sonoda, Takanari Ichikawa, Miki Nakazawa, Miki Fujita, Motoaki Seki, Kazuo Shinozaki, Minami Matsui, Akira Ikeda, Junji Yamaguchi
PLANT AND CELL PHYSIOLOGY, 48, S132, S132, 2007, [Peer-reviewed]
English

Salicylic acid and a chitin elicitor both control expression of the CAD1 gene involved in the plant immunity of Arabidopsis
Tolllokazu Tsutsui, Chlzuko Morita-Yamamuro, Yutaka Asada, Ellchl Minami, Naoto Shibuya, Aklra Ikeda, Junji Yamaguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70, 9, 2042, 2048, Sep. 2006
English, Scientific journal

Differential expression of two fructokinases in Oryza sativa seedlings grown under aerobic and anaerobic conditions
L. Guglielminetti, A. Morita, J. Yamaguchi, E. Loreti, P. Perata, A. Alpi
JOURNAL OF PLANT RESEARCH, 119, 4, 351, 356, Jul. 2006
English, Scientific journal

A transposon-induced spontaneous mutation results in low beta-amylase content in rice
H Saika, M Nakazono, A Ikeda, J Yamaguchi, S Masaki, M Kanekatsu, K Nemoto
PLANT SCIENCE, 169, 1, 239, 244, Jul. 2005
English, Scientific journal

The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain
C Morita-Yamamuro, T Tsutsui, M Sato, H Yoshioka, M Tamaoki, D Ogawa, H Matsuura, T Yoshihara, A Ikeda, Uyeda, I, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 46, 6, 902, 912, Jun. 2005, [Peer-reviewed]
English, Scientific journal

The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain.
MORITA‐YAMAMURO Chizuko, MORITA‐YAMAMURO Chizuko, TSUTSUI Tomokazu, SATO Masanao, YOSHIOKA Hirofumi, TAMAOKI Masanori, OGAWA Daisuke, MATSUURA Hideyuki, YOSHIHARA Teruhiko, IKEDA Akira, UYEDA Ichiro, YAMAGUCHI Junji, YAMAGUCHI Junji
Plant Cell Physiology, 46, 6, 902, 912, 2005

Measurement of the Concentration of Bioactive Gibberellin in Germinating Rice Seed Using the α-Amylase Induction from Aleurone Cells
MITSUNAGA Shin-ichiro, KAWAKAMI Osamu, YAMAGUCHI Junji, MITSUI Toshiaki
Journal of Applied Glycoscience, 52, 4, 399, 402, The Japanese Society of Applied Glycoscience, 2005
Japanese, Measurement of the concentrations of bioactive gibberellin (GA) in germinating cereal seeds is important to grasp the molecular mechanisms of storage starch degradation following the activation of aleurone cells. We investigated a simple method for the estimation of bioactive GA in germinating rice seeds using α-amylase secreted by the aleurone. The α-amylase enzyme activities released from aleurone in response to GA application were plotted against the applied GA concentrations and the amount of α-amylase proteins were visualized on nitrocellulose filter using polyclonal antibodies to compare with those synthesized in germinating seeds. We could define the bioactive GA concentration in a rice seedling ranged from 0.1 to 1 nM and identify the 42 kDa α-amylase isoform, which appears only when the internal GA condition becomes higher than 1 nM. This isoform will be a good index for the analysis of signaling cascades mediated by high concentrations of GA.

The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain.
C Yamamuro, T Tsutsui, Y Asada, H Yoshioka, M Tamaoki, D Ogawa, H Matsuura, T Yoshihara, A Ikeda, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 46, S120, S120, 2005, [Peer-reviewed]
English, Scientific journal

Knock-out of the plastid ribosomal protein S21 causes impaired photosynthesis and sugar-response during germination and seedling development in Arabidopsis thaliana
C Morita-Yamamuro, T Tsutsui, A Tanaka, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 45, 6, 781, 788, Jun. 2004
English, Scientific journal

Low sugar status promotes endogenous ABA level and ABA sensitivity in Arabidopsis
Chizuko Morita-Yamamuro, Paolo Vernieri, Junji Yamaguchi
Plant Biotechnology, 21, 1, 9, 14, Japanese Society for Plant Cell and Molecular Biology, 2004
English, Scientific journal

Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice
Y Sonoda, A Ikeda, S Saiki, T Yamaya, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 44, 12, 1396, 1402, Dec. 2003
English, Scientific journal

Distinct expression and function of three ammonium transporter genes (OsAMT1;1-1;3) in rice
Y Sonoda, A Ikeda, S Saiki, N von Wiren, T Yamaya, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 44, 7, 726, 734, Jul. 2003
English, Scientific journal

Gibberellins are not required for rice germination under anoxia
E Loreti, J Yamaguchi, A Alpi, P Perata
PLANT AND SOIL, 253, 1, 137, 143, Jun. 2003
English, Scientific journal

Characterization of rice functional monosaccharide transporter, OsMST5
B Ngampanya, A Sobolewska, T Takeda, K Toyofuku, J Narangajavana, A Ikeda, J Yamaguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 67, 3, 556, 562, Mar. 2003
English, Scientific journal

Novel gene encoding a Ca2+-binding protein and under hexokinase-dependent sugar regulation
S Otsuki, A Ikeda, T Sunako, S Muto, J Yazaki, K Nakamura, F Fujii, K Shimbo, Y Otsuka, K Yamamoto, K Sakata, T Sasaki, N Kishimoto, S Kikuchi, J Yamaguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 67, 2, 347, 353, Feb. 2003
English, Scientific journal

Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants
A Suenaga, K Moriya, Y Sonoda, A Ikeda, N von Wiren, T Hayakawa, J Yamaguchi, T Yamaya
PLANT AND CELL PHYSIOLOGY, 44, 2, 206, 211, Feb. 2003
English, Scientific journal

Sugar modulation of alpha-amylase genes under anoxia
E Loreti, J Yamaguchi, A Alpi, P Perata
ANNALS OF BOTANY, 91, 2, 143, 148, Jan. 2003
English, Scientific journal

Sugar Transporters Involved in Flowering and Grain Development of Rice
NGAMPANYA Budsaraporn, TAKEDA Taito, NARANGAJAVANA Jarunya, IKEDA Akira, YAMAGUCHI Junji
Journal of Applied Glycoscience, 50, 2, 237, 240, The Japanese Society of Applied Glycoscience, 2003
English, Sucrose and hexoses derived from sucrose breakdown are importance for early grain development in cereals. These assimilates were used to promote division and elongation, differentiation of the cells and deposition in storage organs. OsMST5 (Oryza sativa monosaccharide transporter 5), OsSUT1 (Oryza sativa sucrosetransporter 1) and OsSUT2 (Oryza saliva sucrose transporter 2) have been cloned from rice. All mRNAs of these functional sugar transporters were detected during flowering and grain filling stage. The OsMST5 and OsSUT2 mRNAs were exclusively detectable before the pollinating stage by Northern analysis and the OsSUT2 mRNA was localized in archespores and pollen mother cells by in situ mRNA detection, indicating that these gene products are involved in import of sucrose to promote pollen development at the early stage. Moreover, the OsSUT1 whiclh is known to be phloem companion cell-specific expression in source organs, was also expressed in the sink organs as well as modified aleurone cells of the developing endosperm. These results indicatethat the OsSUT1 plays a role in re-uptake of sucrose from endosperm cavity to accumulate starch in grain filling stage.

The slender rice mutant, with constitutively activated gibberellin signal transduction, has enhanced capacity for abscisic acid level
A Ikeda, Y Sonoda, P Vernieri, P Perata, H Hirochika, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 43, 9, 974, 979, Sep. 2002
English, Scientific journal

Functional analysis of rice ammonium transporter gene OsAMT1;3(Abstracts of The 37th Annual Meeting of the Japanese Society for Chemical Regulation of Plants)
Saiki Satomi, Sonoda Yutaka, Ikeda Akira, Yamaya Tomoyuki, Yamaguchi Junji
Regulation of Plant Growth & Development, 37, 2, 221, 221, The Japanese Society for Chemical Regulation of Plants, 2002
Japanese

slender rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8
A Ikeda, M Ueguchi-Tanaka, Y Sonoda, H Kitano, M Koshioka, Y Futsuhara, M Matsuoka, J Yamaguchi
PLANT CELL, 13, 5, 999, 1010, May 2001
English, Scientific journal

Sugar transporters involved in flowering and grain development of rice
T Takeda, K Toyofuku, C Matsukura, J Yamaguchi
JOURNAL OF PLANT PHYSIOLOGY, 158, 4, 465, 470, Apr. 2001
English, Scientific journal

Polymorphism in rice amylases at an early stage of seed germination
S Mitsunaga, O Kawakami, T Numata, J Yamaguchi, K Fukui, T Mitsui
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 65, 3, 662, 665, Mar. 2001
English, Scientific journal

Sugar uptake and transport in rice embryo. Expression of companion cell-specific sucrose transporter (OsSUT1) induced by sugar and light
C Matsukura, T Saitoh, T Hirose, R Ohsugi, P Perata, J Yamaguchi
PLANT PHYSIOLOGY, 124, 1, 85, 93, Sep. 2000, [Peer-reviewed]
English, Scientific journal

Characterization and expression of monosaccharide transporters (OsMSTs) in rice
K Toyofuku, M Kasahara, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 41, 8, 940, 947, Aug. 2000, [Peer-reviewed]
English, Scientific journal

Distribution and characterization of enzymes causing starch degradation in rice (Oryza sativa Cv. Koshihikari)
Motoko Awazuhara, Atsuko Nakagawa, Junji Yamaguchi, Toru Fujiwara, Hiroaki Hayashi, Keiko Hatae, Mitsuo Chino, Atsuko Shimada
Journal of Agricultural and Food Chemistry, 48, 2, 245, 252, Feb. 2000, [Peer-reviewed]
English, Scientific journal

Glucose modulates the abscisic acid-inducible Rab16A gene in cereal embryos
K Toyofuku, E Loreti, P Vernieri, A Alpi, P Perata, J Yamaguchi
PLANT MOLECULAR BIOLOGY, 42, 3, 451, 460, Feb. 2000, [Peer-reviewed]
English, Scientific journal

Expression of α-amylase isoforms and the RAmy1A gene in rice (Oryza sativa L.) during seed germination, and its relationship with coleoptile length in submerged soil
J. Huang, K. Toyofuku, J. Yamaguchi, S. Akita
Plant Production Science, 3, 1, 32, 37, Crop Science Society of Japan, 2000, [Peer-reviewed]
English, Scientific journal

Characterization of isoforms of hexose kinases in rice embryo
L Guglielminetti, P Perata, A Morita, E Loreti, J Yamaguchi, A Alpi
PHYTOCHEMISTRY, 53, 2, 195, 200, Jan. 2000, [Peer-reviewed]
English, Scientific journal

Glucose repression of a-amylase in barley embryos is independent of GAMYB transcription.
Loreti;E, C., Matsukura, F., Gubler, A., Alpi, J., Yamaguchi, P., Perata
Plant Molecular Biology, 44, 85-90, 90, Jan. 2000, [Peer-reviewed]
English, Scientific journal

Transverse vein differentiation associated with gas space formation - Fate of the middle cell layer in leaf sheath development of rice
C Matsukura, M Kawai, K Toyofuku, RA Barrero, H Uchimiya, J Yamaguchi
ANNALS OF BOTANY, 85, 1, 19, 27, Jan. 2000, [Peer-reviewed]
English, Scientific journal

Differences in sugar regulation between two isoforms of alpha-amylase in suspension-cultured cells of rice.　　　　　　　　　　　　　　　
N. Geshi, T. Mitsui, T. Akazawa, J. Yamaguchi
J. Appl. Glycosci., 46, 2, 111, 119, Dec. 1999, [Peer-reviewed]
English, Scientific journal

Auxin and morphogenesis of rice plant root. Analysis of GASR1 gene and protein.
池田 亮, 山口 淳二
Root Research, 8, 1, 9, 12, Japanese Society for Root Research, 08 Mar. 1999
Japanese

Reducing-agents-mediated solubilization and activation of debranching enzyme (pullulanase) in rice flour
Wakako Takeuchi, Hironori Masui, Junji Yamaguchi
Bioscience, Biotechnology and Biochemistry, 63, 3, 510, 514, 01 Jan. 1999, [Peer-reviewed]
English, Scientific journal

Changes in α-amylase isoforms during emergence of rice in submerged soil
Jirong Huang, Junji Yamaguchi, Shigemi Akita
Plant Production Science, 2, 1, 12, 13, Crop Science Society of Japan, 1999, [Peer-reviewed]
English, Scientific journal

Sugar repression of a-amylase genes in rice embryos.　　　　　　　　　　　　　　　
Yamaguchi;J, K., Toyofuku, A., Morita, A., Ikeda, C., Matsukura, P., Perata
8th International Symposium on Pre-harvest Sprouting in Cereals, 136-145, Jan. 1999
English, International conference proceedings

Characterization of beta-amylase and its deficiency in various rice cultivars
J Yamaguchi, S Itoh, T Saitoh, A Ikeda, T Tashiro, Y Nagato
THEORETICAL AND APPLIED GENETICS, 98, 1, 32, 38, Jan. 1999, [Peer-reviewed]
English, Scientific journal

Analysis of embryo-specific alpha-amylase using isolated mature rice embryos
J Yamaguchi
BREEDING SCIENCE, 48, 4, 365, 370, Dec. 1998, [Peer-reviewed]
English, Scientific journal

Detection of proteins binding to the promoter region DNA using a nonradioactive gel-retardation assay
S Mitsunaga, K Fukui, H Ohyama, J Yamaguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62, 9, 1812, 1814, Sep. 1998, [Peer-reviewed]
English, Scientific journal

Promotion of leaf sheath growth by gibberellic acid in a dwarf mutant of rice
C Matsukura, S Itoh, K Nemoto, E Tanimoto, J Yamaguchi
PLANTA, 205, 2, 145, 152, Jun. 1998, [Peer-reviewed]
English, Scientific journal

Promoter elements required for sugar-repression of the RAmy3D gene for alpha-amylase in rice
K Toyofuku, T Umemura, J Yamaguchi
FEBS LETTERS, 428, 3, 275, 280, May 1998, [Peer-reviewed]
English, Scientific journal

Sugar sensing and alpha-amylase gene repression in rice embryos
T Umemura, P Perata, Y Futsuhara, J Yamaguchi
PLANTA, 204, 4, 420, 428, Apr. 1998, [Peer-reviewed]
English, Scientific journal

Temporal and spatial expression of the alpha-amylase gene during seed germination in rice and barley
N Sugimoto, G Takeda, Y Nagato, J Yamaguchi
PLANT AND CELL PHYSIOLOGY, 39, 3, 323, 333, Mar. 1998, [Peer-reviewed]
English, Scientific journal

Functional dissection of a sugar-repressed alpha-amylase gene (RAmylA) promoter in rice embryos
A Morita, T Umemura, M Kuroyanagi, Y Futsuhara, P Perata, J Yamaguchi
FEBS LETTERS, 423, 1, 81, 85, Feb. 1998, [Peer-reviewed]
English, Scientific journal

Analysis of embryo-specific α-amylase using isolated mature rice embryos
J. Yamaguchi
Breeding Science, 48, 4, 365, 370, Japanese Society of Breeding, 1998, [Peer-reviewed]
English, Scientific journal

Effect of anoxia on gibberellic acid-induced protease and beta-amylase processing in barley seeds
E Loreti, L Guglielminetti, J Yamaguchi, S Gonzali, A Alpi, P Perata
JOURNAL OF PLANT PHYSIOLOGY, 152, 1, 44, 50, Jan. 1998, [Peer-reviewed]
English, Scientific journal

Starch degradation during gibberellin-induced leaf sheath growth in rice.　　　　　　　　　　　　　　　
Matsukura;C, J., Yamaguchi, 松倉, 千昭
Rice Genetics Newsletter, 15, 114-117, Jan. 1998, [Peer-reviewed]
English, Scientific journal

Sugar repression of a gibberellin-dependent signaling pathway in barley embryos.
Perata;P, C., Matsukura, P., Vernieri, J., Yamaguchi
Plant Cell, 9, 2197-2208, 2208, Jan. 1997, [Peer-reviewed]
English, Scientific journal

The first leaf growth promoted by gibberellins in dwarf mutant of rice.　　　　　　　　　　　　　　　
Matsukura;C, J., Yamaguchi, 松倉, 千昭
Rice Genetics Newsletter, 14, 153-155, Jan. 1997, [Peer-reviewed]
English, Scientific journal

Physicochemical and serological characterization of rice alpha-amylase isoforms and identification of their corresponding genes
T Mitsui, J Yamaguchi, T Akazawa
PLANT PHYSIOLOGY, 110, 4, 1395, 1404, Apr. 1996, [Peer-reviewed]
English, Scientific journal

The same nuclear proteins bind to the 5′-flanking regions of genes for the rice seed storage protein: 16 kDa albumin, 13 kDa prolamin and type II glutelin
Masayuki Nakase, Takehisa Yamada, Takahiro Kira, Junji Yamaguchi, Naohito Aoki, Ryo Nakamura, Tsukasa Matsuda, Takahiro Adachi
Plant Molecular Biology, 32, 4, 621, 630, Springer Netherlands, 1996, [Peer-reviewed]
English, Scientific journal

Changes in α-amylase activity during plant regeneration from rice calli
Toshinori Abe, Masumi Kudo, Yasuhiro Oka, Junji Yamaguchi, Takeo Sasahara
Journal of Plant Physiology, 149, 5, 592, 598, Elsevier GmbH, 1996, [Peer-reviewed]
English, Scientific journal

Amylolytic activities in cereal seeds under aerobic and anaerobic conditions
Lorenzo Guglielminetti, Junji Yamaguchi, Pierdomenico Perata, Amedeo Alpi
Plant Physiology, 109, 3, 1069, 1076, American Society of Plant Biologists, 1995, [Peer-reviewed]
English, Scientific journal

DEVELOPMENTAL AND HORMONAL-REGULATION OF RICE ALPHA-AMYLASE(RAMY1A)-GUSA FUSION GENES IN TRANSGENIC RICE SEEDS
K ITOH, J YAMAGUCHI, N HUANG, RL RODRIGUEZ, T AKAZAWA, K SHIMAMOTO
PLANT PHYSIOLOGY, 107, 1, 25, 31, Jan. 1995, [Peer-reviewed]
English, Scientific journal

SEQUENCE-SPECIFIC INTERACTIONS OF A NUCLEAR-PROTEIN FACTOR WITH THE PROMOTER REGION OF A RICE GENE FOR ALPHA-AMYLASE, RAMY3D
S MITSUNAGA, RL RODRIGUEZ, J YAMAGUCHI
NUCLEIC ACIDS RESEARCH, 22, 11, 1948, 1953, Jun. 1994, [Peer-reviewed]
English, Scientific journal

IDENTIFICATION AND CHARACTERIZATION OF GIBBERELLIN-INSENSITIVE MUTANTS SELECTED FROM AMONG DWARF MUTANTS OF RICE
S MITSUNAGA, T TASHIRO, J YAMAGUCHI
THEORETICAL AND APPLIED GENETICS, 87, 6, 705, 712, Jan. 1994, [Peer-reviewed]
English, Scientific journal

EFFECT OF ANOXIA ON THE INDUCTION OF ALPHA-AMYLASE IN CEREAL SEEDS
P PERATA, N GESHI, J YAMAGUCHI, T AKAZAWA
PLANTA, 191, 3, 402, 408, Aug. 1993, [Peer-reviewed]
English, Scientific journal

INDUCTION OF ALPHA-AMYLASE IS REPRESSED BY UNICONAZOLE, AN INHIBITOR OF THE BIOSYNTHESIS OF GIBBERELLIN, IN A DWARF MUTANT OF RICE, WAITO-C
S MITSUNAGA, J YAMAGUCHI
PLANT AND CELL PHYSIOLOGY, 34, 2, 243, 249, Mar. 1993, [Peer-reviewed]
English, Scientific journal

EFFECT OF ANOXIA ON STARCH BREAKDOWN IN RICE AND WHEAT SEEDS
P PERATA, J POZUETAROMERO, T AKAZAWA, J YAMAGUCHI
PLANTA, 188, 4, 611, 618, Nov. 1992, [Peer-reviewed]
English, Scientific journal

COMPLEMENTARY-DNA SEQUENCE OF AN ADENYLATE TRANSLOCATOR FROM ARABIDOPSIS-THALIANA
A SAINTGUILY, PY LIM, C CHEVALIER, J YAMAGUCHI, T AKAZAWA
PLANT PHYSIOLOGY, 100, 2, 1069, 1071, Oct. 1992, [Peer-reviewed]
English, Scientific journal

ARTIFACTUAL DETECTION OF ADP-DEPENDENT SUCROSE SYNTHASE IN CRUDE PLANT-EXTRACTS
P PERATA, J POZUETAROMERO, J YAMAGUCHI, T AKAZAWA
FEBS LETTERS, 309, 3, 283, 287, Sep. 1992, [Peer-reviewed]
English, Scientific journal

Isolation and Characterization of Nuclei from Rice Embryos
Junji Yamaguchi, Poh-Yam Lim, Takashi Akazawa, Kazunii Aratani
Cell Structure and Function, 17, 2, 87, 92, 1992, [Peer-reviewed]
English, Scientific journal

Nucleotide sequence of a cDNA for catalase from Arabidopsis thaliana
Christian Chevalier, Junji Yamaguchi, Peter McCourt
Plant Physiology, 99, 4, 1726, 1728, American Society of Plant Biologists, 1992, [Peer-reviewed]
English, Scientific journal

The preparation of high-molecular-weight DNA from rice and its analysis by pulsed-field gel electrophoresis
Shoji Hatano, Junji Yamaguchi, Atsushi Hirai
Plant Science, 83, 1, 55, 64, 1992, [Peer-reviewed]
English, Scientific journal

ADPG FORMATION BY THE ADP-SPECIFIC CLEAVAGE OF SUCROSE-REASSESSMENT OF SUCROSE SYNTHASE
J POZUETAROMERO, J YAMAGUCHI, T AKAZAWA
FEBS LETTERS, 291, 2, 233, 237, Oct. 1991, [Peer-reviewed]
English, Scientific journal

ANALYTICAL STUDIES ON THE MICROBODY TRANSITION .8. BIOSYNTHESIS AND INTRACELLULAR-TRANSPORT OF GLYOXYSOMAL MALATE-DEHYDROGENASE IN GERMINATING PUMPKIN COTYLEDONS
J YAMAGUCHI, H MORI, M NISHIMURA
FEBS LETTERS, 213, 2, 329, 332, Mar. 1987, [Peer-reviewed]
English, Scientific journal

Distribution of 59- and 55-kDa catalase in dark- and light-grown pumpkin and various other plant tissues
Junji Yamaguchi, Mikio Nishimura, Takashi Akazawa
Plant and Cell Physiology, 28, 2, 219, 226, Japanese Society of Plant Physiologists, Mar. 1987, [Peer-reviewed]
English

PURIFICATION AND CHARACTERIZATION OF HEME-CONTAINING LOW-ACTIVITY FORM OF CATALASE FROM GREENING PUMPKIN COTYLEDONS
J YAMAGUCHI, M NISHIMURA, T AKAZAWA
EUROPEAN JOURNAL OF BIOCHEMISTRY, 159, 2, 315, 322, Sep. 1986, [Peer-reviewed]
English, Scientific journal

ANALYTICAL STUDIES ON MICROBODY TRANSITION .5. IMMUNOCYTOCHEMICAL ANALYSIS SHOWS THAT GLYOXYSOMES ARE DIRECTLY TRANSFORMED TO LEAF PEROXISOMES DURING GREENING OF PUMPKIN COTYLEDONS
M NISHIMURA, J YAMAGUCHI, H MORI, T AKAZAWA, S YOKOTA
PLANT PHYSIOLOGY, 81, 1, 313, 316, May 1986
English

ANALYTICAL STUDIES ON MICROBODY TRANSITION .3. PURIFICATION OF GLYOXYSOMAL CATALASE AND IMMUNOCHEMICAL COMPARISON OF GLYOXYSOMAL AND LEAF PEROXISOMAL CATALASE IN GERMINATING PUMPKIN COTYLEDONS
J YAMAGUCHI, M NISHIMURA
PLANT PHYSIOLOGY, 74, 2, 261, 267, 1984
English, Scientific journal

ANALYTICAL STUDIES ON THE MICROBODY TRANSITION .4. MATURATION OF CATALASE PRECURSOR PROCEEDS TO A DIFFERENT EXTENT IN GLYOXYSOMES AND LEAF PEROXISOMES OF PUMPKIN COTYLEDONS
J YAMAGUCHI, M NISHIMURA, T AKAZAWA
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 81, 15, 4809, 4813, 1984
English, Scientific journal

Methods for elucidation of plant senescence in response to C/N-nutrient balance
Shoki Aoyama, Junji Yamaguchi, Takeo Sato, Methods in Molecular Biology, 1744, 151, 159, 2018, [Peer-reviewed]
Humana Press Inc., English

P022 Anthranilic acid and its derivatives promote rooting specifically to adventitious root
Maki Yuko, Soejima Hiroshi, Sato Takeo, Watahiki Masaaki, Yamaguchi Junji, 植物化学調節学会研究発表記録集, 50, 40, 40, 01 Oct. 2015
The Japanese Society for Chemical Regulation of Plants, Japanese

4-1-7 シロイヌナズナにおける硝酸シグナル応答型転写因子NLPの新規標的遺伝子BT群の機能解析(4-1 植物の多量栄養素,2015年度京都大会)
前川 修吾, 吉岡 希, 小西 美稲子, 石田 哲也, 加藤 祐樹, 佐々木 勇樹, 佐藤 長緒, 山口 淳二, 柳澤 修一, 日本土壌肥料学会講演要旨集, 61, 50, 50, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

4-1-9 植物の炭素/窒素栄養バランス応答を制御する膜局在型ユビキチンリガーゼATL31の機能解析(4-1 植物の多量栄養素,2015年度京都大会)
佐藤 長緒, HUARANCCA REYES Thais, 植村 知博, 深尾 陽一朗, 山口 淳二, 日本土壌肥料学会講演要旨集, 61, 50, 50, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

4-1-10 大気中二酸化炭素と窒素栄養バランスに応答した老化制御におけるユビキチンリガーゼATL31の機能解析(4-1 植物の多量栄養素,2015年度京都大会)
青山 翔紀, Reyes Thais Huarancca, Guglielminetti Lorenzo, 陸 宇, 森田 嘉恵, 佐藤 長緒, 山口 淳二, 日本土壌肥料学会講演要旨集, 61, 51, 51, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

植物免疫における膜局在型ユビキチンリガーゼATL31のリン酸化とその機能解析
安田盛貴, 長谷川陽子, 門田康弘, 深尾陽一朗, 佐藤長緒, 山口淳二, 日本植物生理学会年会要旨集, 56th, 2015

Exploration of substrates for a membrane-localized ubiquitin ligase in plant innate immunity
安田盛貴, 長谷川陽子, 門田康弘, 深尾陽一朗, 佐藤長緒, 山口淳二, 日本プロテオーム学会大会プログラム・抄録集, 2015 (Web), 2015

Assay for proteasome-dependent protein degradation and ubiquitinated proteins
Takeo Sato, Kaori Sako, Junji Yamaguchi, Methods in Molecular Biology, 1072, 655, 663, 2014, [Peer-reviewed]
Humana Press Inc., English

C/Nバランス調節による植物の代謝・成長戦略
佐藤長緒, 山口淳二, 化学と生物, 51, 11, 763, 772, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry, Japanese

32. Phenyllactic acid, root-promoting substance isolated from Bokashi, exhibits synergistic effect with Tryptophan
Maki Yuko, Soejima Hiroshi, Kitamura Toru, Sugiyama Tamizi, Yamaguchi Junji, 植物化学調節学会研究発表記録集, 47, 49, 49, 05 Oct. 2012
The Japanese Society for Chemical Regulation of Plants, Japanese

The new findings of ubiquitin-proteasome system from yeast to higher organisms
YAMAGUCHI Junji, HATAKEYAMA Shigetsugu, 生化學, 84, 6, 407, 408, 25 Jun. 2012
Japanese

シロイヌナズナ26Sプロテアソームを介したDNAメチル化制御機構の解析
佐古香織, 金井知行, 加藤絵里子, 綿引雅昭, 山口淳二, 日本植物生理学会年会要旨集, 52nd, 169, 11 Mar. 2011
Japanese

低温ストレス応答に関わるmRNA分解速度の調節
千葉由佳子, 千葉由佳子, 峯田克彦, 平井優美, 鈴木悠也, 山口淳二, GREEN Pamela J, 内藤哲, 日本植物生理学会年会要旨集, 52nd, 246, 11 Mar. 2011
Japanese

P12-6 植物の低温ストレス応答機構とRNA制御(ポスター紹介,12.植物の代謝成分と農産物の品質,2010年度北海道大会)
千葉 由佳子, 峯田 克彦, 平井 優美, 山口 淳二, Green Pamela J., 内藤 哲, 日本土壌肥料学会講演要旨集, 56, 95, 95, 07 Sep. 2010
一般社団法人日本土壌肥料学会, Japanese

26Sプロテアソームサブユニットを介した遺伝子発現制御機構の解析
眞木祐子, 佐古香織, 綿引雅昭, GOTO Derek, 山口淳二, 日本植物生理学会年会要旨集, 51st, 114, 12 Mar. 2010
Japanese

Towards understanding how plant cell function is changed during root-knot nematode parasitism
Goto Naoko, Maruyama Yosuke, Noorul Amin Arshana Nor, Asamizu Erika, Ezura Hiroshi, Osaki Mitsuru, Yamaguchi Junji, Goto Derek, Plant and Cell Physiology Supplement, 2010, 945, 945, 2010
Root-knot nematodes (RKNs) are a major parasite of plants, causing approximately 5% loss of total agriculture world-wide. RKN are sedentary endoparasites that establish a single permanent feeding site within plant roots as a juvenile, and then spend their whole remaining life-cycle at this single site. This is achieved by inducing normal plant cells to change into a new specialised cell-type, a Giant Cell (GC), that functions as a nutrient transfer cell. The pathway for formation of a GC from a normal plant cell remains unclear, mainly due to the difficulties in directly studying the early infection stages hidden within the host root. Using the tomato variety Micro-Tom as a model system, we have initiated a forward genetics approach to identify host genes involved in RKN-induced GC formation and are also studying the early GC cells directly. Lab home page: http://gotolab.cris.hokudai.ac.jp, The Japanese Society of Plant Physiologists

タバコの液胞膜型ショ糖トランスポーター(NtSUT4)の同定と機能の生理学的解析
大窪(栗原) 恵美子, 桧垣匠, 桧垣匠, 栗原志夫, 朽名夏麿, 朽名夏麿, 山口淳二, 馳澤盛一郎, 馳澤盛一郎, 日本植物生理学会年会要旨集, 51st, 2010

シロイヌナズナDEAR1はDREBドメインとEARモチーフを持つ転写抑制因子であり,低温応答と病原体抵抗性を制御する
筒井友和, 加藤航, 矢元奈津子, 浅田裕, 城所聡, 篠崎和子, 玉置雅紀, 池田亮, 山口淳二, 日本植物生理学会年会要旨集, 49th, 2008

Functions of plant proteasome
Sako Kaori, Sonoda Yutaka, Ikeda Akira, Yamaguchi Junji, Regulation of Plant Growth & Development, 42, 1, 54, 60, 2007
26Sプロテアソームは,様々な細胞制御に関与する「ユビキチン・プロテアソームシステムによるタンパク質の能動的分解」の実質を担う巨大タンパク質複合体である.シロイヌナズナでは,このユビキチン・プロテアソームシステムに関連する遺伝子群が全ゲノムの5%以上を占める.これは,ゲノムワイドでみた植物の特徴の一つであり,植物の示す優れた環境適応能力の一翼を担う分子機構と考えられている.本稿では,ユビキチン・プロテアソームシステムおよび植物プロテアソームの概要について記した後,19S複合体(19Sプロテアソーム)を構成するサブユニット群の機能の多様性について解説する.植物では,サブユニット間だけでなく,サブユニットのパラログ分子間でも明確な機能の違いが生じる.この具体例として,著者らが行っているRPT2に焦点をあてた研究例を紹介する.RPT2はエンドリデュプリケーション(細胞分裂を伴わないDNA複製)制御に関与することが明らかとなった.細胞サイズ制御の問題とあわせて解説する., The Japanese Society for Chemical Regulation of Plants, Japanese

Salicylic acid and a chitin elicitor both control expression of the CAD1 gene involved in the plant immunity of Arabidopsis
Tolllokazu Tsutsui, Chlzuko Morita-Yamamuro, Yutaka Asada, Ellchl Minami, Naoto Shibuya, Aklra Ikeda, Junji Yamaguchi, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70, 9, 2042, 2048, Sep. 2006
English

Loss-of-function mutation of rice hexokinase OsHXK1 does not affect sugar regulation of α-amylase RAmy3D　　　　　　　　　　　　　　　
Rice Genetics Newsletter, 21, 90, 92, 2004

Ammonium Uptake and Assimilation in Rice
Yamaguchi J., Sonoda Y., Iwata A., Ikeda A., Tsutsui T., Morita-Yamamuro C., Yao S.-G., Matsuhashi S., Fujimaki S., Sakamoto K., Arakawa K., Kume T., JAERI-Review, 2004, 025, 122, 124, 2004
独立行政法人日本原子力研究開発機構, English

炭素源分配の制御機構--C/Nバランスによる制御を中心として (植物の代謝コミュニケーション--植物分子生理学の新展開) -- (クロストーク)
池田 亮, 園田 裕, 山口 淳二, 蛋白質核酸酵素, 48, 15, 1980,2103, 2112, Nov. 2003
共立出版, Japanese

Short-distance and Long-distance Transport of Photosynthetic Assimilates
YAMAGUCHI Junji, Journal of Applied Glycoscience, 50, 4, 91, 92, 20 Oct. 2003
The Japanese Society of Applied Glycoscience, Japanese

Gibberellin signaling factors : all about DELLA family(Recent trends of plant hormone research I Perception and signal transduction Preface)
Ikda Akira, Yamamuro Chizuko, Yamaguchi Junji, Regulation of Plant Growth & Development, 38, 1, 36, 47, 30 May 2003
The Japanese Society for Chemical Regulation of Plants, Japanese

炭素源分配の制御機構－C/Nバランスによる制御を中心として－　　　　　　　　　　　　　　　
蛋白質核酸酵素, 48, 2103, 2112, 2003

Negative-feedback regulation of ammonium uptake and translocation in rice under ambient and elevated CO2 conditions　　　　　　　　　　　　　　　
JAERI-Review, 2003, 033, 126, 128, 2003

Gibberellin signaling factors : all about DELLA family(Recent trends of plant hormone research I Perception and signal transduction Preface)
Ikda Akira, Yamamuro Chizuko, Yamaguchi Junji, Regulation of Plant Growth & Development, 38, 1, 36, 47, 2003
The Japanese Society for Chemical Regulation of Plants, Japanese

Functional analysis of rice ammonium transporter gene OsAMT1;3
Saiki Satomi, Sonoda Yutaka, Ikeda Akira, Yamaya Tomoyuki, Yamaguchi Junji, 植物化学調節学会研究発表記録集, 37, 131, 131, 31 Oct. 2002
We have already isolated two ammonium transporter genes OsAMT1;1,and 1;2 from rice(Oryza sativa L.) and characterized their distinct functions. Furthermore, we isolated an additional ammonium transporter gene OsAMT1;3 and attempted to characterize the gene. OsAMT1;3 gene, as same as OsAMT1;1 and 1;2,was classified into AMT1 gene family, and hydrophobic profile of the OsAMT1;3 -deduced amino acid sequence revealed that the translation product is an integrated protein having 10-11 membrane spanning domains. We examined expression pattern of OsAMT1;3 by RT-PCR method. OsAMT1;3 transcript was detected only in root, and the transcript was higher level under nitrogen deficiency. When rice with pretreatment under nitrogen deficiency was supplied with either NH_4^+ or NO_3^-, OsAMT1;3 expression was repressed by the both treatments compared with that under the nitrogen deficiency. Thus, these results indicate that the OsAMT1;3 gene expression is regulated under nitrogen status in plants and/or rhizosphere, and the protein is involved in the uptake of NH_4^+ under nitrogen deficiency. OsAMT1;3 was functionally complement a yeast mutant which is deficient in NH_4^+ uptake, indicating that the gene encodes functional ammonium transporter., The Japanese Society for Chemical Regulation of Plants, Japanese

イネのアンモニウムトランスポーターの機能解析
池田 亮, 園田 裕, 斎木 里文, 山口 淳二, 根の研究 = Root research, 11, 2, 93, 93, 26 Jun. 2002
Japanese

機能性食品としての発芽玄米を用いた糖質とデンプン分解酵素の変動に関するプロファイリング
山口 淳二, 竹内 若子, 升井 洋至, 年報, 2002, 317, 324, 2002
飯島記念食品科学振興財団, Japanese

根のアンモニウムイオン取り込みに関与する遺伝子群
斎木里文, 園田裕, 池田亮, 山口淳二, 農業および園芸, 77, 1, 39, 47, 2002
養賢堂, Japanese

Characterization of OsSUT2 cDNA expressed before flowering stage of rice　　　　　　　　　　　　　　　
Rice Genetics Newsletter, 19, 49, 52, 2002

高圧処理米粉におけるデンプン分解酵素について : 近畿支部 第28回研究発表会講演要旨
升井 洋至, 竹内 若子, 山口 淳二, 日本調理科学会誌, 34, 4, 116, 116, 2001
日本調理科学会, Japanese

EXPRESSION OF A NOVEL GENE ENCODING Ca^<2+>-BINDING PROTEIN IS INDUCED BY SUGARS :
OTSUKI Shigeo, MATSUKURA Chiaki, MUTO Shoshi, YAMAGUCHI Junji, Plant and cell physiology, 42, s102, 2001
Japanese Society of Plant Physiologists, English

CHARACTERIZATION OF RICE MONOSACCHARIDE TRANSPORTER, OSMST3 :
TOYOFUKU Kyoko, TANIMOTO Eiichi, FUKUSHIMA Kazuhiko, YAMAGUCHI Junji, Plant and cell physiology, 42, s211, 2001
Japanese Society of Plant Physiologists, English

Molecular characterization of sugar transporters involved in flowering and grain development of rice :
Takeda Taito, Toyofuku Kyoko, Sobolewska Anna, Matsukura Chiaki, Yamaguchi Junji, Plant and cell physiology, 42, s211, 2001
Japanese Society of Plant Physiologists, English

植物の物質輸送と根における窒素源の取り込み
池田 亮, 園田 裕, 山谷 知行, 山口 淳二, 根の研究 = Root research, 9, 4, 190, 191, 20 Dec. 2000
Japanese

Functional analysis of ammomium transporter in rice
SONODA Y., IKEDA A., ISHIWATA A., YAMAGUCHI J., 育種学研究 = Breeding research, 2, 2, 62, 62, 25 Sep. 2000
Japanese

Analysis of sugar transporters during seed development in rice
TAKEDA T., TOYOFUKU K., YAMAGUCHI J., 育種学研究 = Breeding research, 2, 2, 240, 240, 25 Sep. 2000
Japanese

イネの根に特異的なアンモニウムトランスポーター遺伝子の機能解析
園田 裕, 池田 亮, 山口 淳二, 根の研究 = Root research, 9, 2, 96, 96, 22 Jun. 2000
Japanese

Expression of the α-Amylase Gene RAmy3D in Rice (Oryza sativa L.) under Aerobic, Hypoxic and Anoxic Conditions
HUANG Jirong, YAMAGUCHI Junji, AKITA Shigemi, Plant production science, 3, 3, 213, 218, 01 Jun. 2000
Expression of the α-amylase gene RAmy3D was studied in aerobic, hypoxic, and anoxic conditions using the rice cultivars Suweon 287 and Nipponbare by in situ hybridization. In the aerobic condition, the initial expression of RAmy3D in the scutellar epithelium and its later expression in the aleurone layer were consistent with the previous reports. However, hypoxia delayed the expression of RAmy3D in the scutellar epithelium, and blocked expression in the aleurone layer. In anoxia, RAmy3D expression in cv.Nipponbare was similar to that in hypoxia, but that in cv.Suweon 287 was significantly suppressed 4 d after seeding. These results suggest that the expression of the RAmy3D gene is also affected by anoxia. Considering the results of our previous report together, RAmy3D-encoded isoforms in anoxia may be mainly regulated at the post-transcriptional level. A possible process in the regulation of RAmy3D expression in germinating seeds is discussed., The Crop Science Society of Japan, English

Expression of genes involved in sugar metabolism and sugar transport in vascular bundle in rice leaf.
MATSUKURA C., HIROSE T., OHSUGI R., YAMAGUCHI J., 育種学研究 = Breeding research, 2, 1, 67, 67, Apr. 2000
Japanese

Constitutive gibberellin response mutant, slender rice is caused by null mutation of SLR gene, an orthologue of RHT-1, Green revolution gene.
IKEDA A., UEGUCHI-TANAKA M., SONODA Y., KITANO H., FUTSUHARA Y., MATSUOKA M., YAMAGUCHI J., 育種学研究 = Breeding research, 2, 1, 100, 100, Apr. 2000
Japanese

Enriched rice! New aspects of plant metabolic engineering for improving sugar sensors
OTSUKI Shigeo, YAMAGUCHI Junji, バイオサイエンスとインダストリー = Bioscience & industry, 58, 4, 38, 39, 01 Apr. 2000
Japanese

植物の根に関する諸問題(79)根の側根形成と窒素の取り込みに関する遺伝子群
池田 亮, 園田 裕, 山口 淳二, 農業および園芸, 75, 2, 301, 308, Feb. 2000
養賢堂, Japanese

イネ登熟種子における糖輸送-デンプン蓄積の制御機構--糖誘導型カルシウム結合性タンパク質OsSUR1の機能解析
山口 淳二, 年報, 2000, 108, 110, 2000
飯島記念食品科学振興財団, Japanese

FUNCTIONAL ANALYSIS OF HEXOKINASE IN RICE :
SUNAKO Tomomi, MORITA Akiyoshi, YAMAGUCHI Junji, Plant and cell physiology, 41, s72, 2000
Japanese Society of Plant Physiologists, English

MOLECULAR MECHANISMS FOR DETERMINATION OF PLANT HEIGHT BY GIBBERELLINS :
IKEDA Akira, MATSUKURA Chiaki, YAMAGUCHI Junji, Plant and cell physiology, 41, s2, 2000
Japanese Society of Plant Physiologists, English

CONSTITUTIVE GIBBERELLIN RESPONSE MUTANT, SLENDER RICE IS CAUSED BY NULL MUTATION OF SLR GENE, AN ORTHOLOGUE OF GAI/RGA/RHT/D8 :
IKEDA Akira, TANAKA Miyako, SONODA Yutaka, KITANO Hidemi, FUTSUHARA Yuzo, MATSUOKA Makoto, YAMAGUCHI Junji, Plant and cell physiology, 41, s197, 2000
Japanese Society of Plant Physiologists, English

ANALYSES OF SUGAR TRANSPORT VIA VASCULAR SYSTEM IN RICE :
MATSUKURA Chiaki, HIROSE Tatsuro, OHSUGI Ryu, YAMAGUCHI Junji, Plant and cell physiology, 41, s28, 2000
Japanese Society of Plant Physiologists, English

オーキシンとイネの根の形態形成 : GASR1 遺伝子・タンパク質の解析
池田 亮, 蓬原 雄三, 山口 淳二, 根の研究 = Root research, 8, 4, 136, 136, 14 Dec. 1999
Japanese

植物の篩管輸送と糖転流 スクローストランスポーターはどのように発現し機能するか
松倉 千昭, 山口 淳二, 化学と生物, 37, 10, 639, 642, 25 Oct. 1999
学会出版センタ-, Japanese

イネの恒常的ジベレリン反応性突然変異体,"slender mutant"の形質発現と遺伝様式
池田 亮, 田中 繁男, 山口 淳二, 名城大学農学部学術報告, 35, 7, 13, Mar. 1999
名城大学農学部, Japanese

イネの恒常的ジベレリン感受性突然変異体,"slender mutant"におけるジベレリンシグナル伝達系の解析
池田 亮, 田中 繁男, 山口 淳二, 名城大学農学部学術報告, 35, 15, 22, Mar. 1999
名城大学農学部, Japanese

ISOFORM AND cDNA CHARACTERIZATION OF HEXOSE KINASES IN RICE EMBRYO
MORITA Akiyoshi, GUGLIELMINETTI Lorenzo, PERATA Pierdomenico, LORETI Elena, ALPI Amedio, YAMAGUCHI Junji, Plant and cell physiology, 40, s115, s115, Mar. 1999
English

MOLECULAR MECHANISMS OF SUGAR TRANSPORT 2 -FUNCTIONAL ANALYSIS OF MONOSACCHARIDE TRANSPORTER cDNAs OF RICE USING YEAST MUTAMT-
TOYOFUKU Kyoko, KASAHARA Michihiro, YAMAGUCHI Junji, Plant and cell physiology, 40, s170, s170, Mar. 1999
English

SLENDER RICE, A CONSTITUTIVE GIBBERELLIN RESPONSE MUTANT
IKEDA Akira, UEGUCHI-TANAKA Miyako, KITANO Hidemi, KOSHIOKA Masaji, FUTSUHARA Yuzo, MATSUOKA Makoto, YAMAGUCHI Junji, Plant and cell physiology, 40, s50, s50, Mar. 1999
English

TRANSVERSE VEIN DIFFERENTIATION ASSOCIATED WITH THE AIR SPACE FORMATION - CELL FATE OF MIDDLE LAYER IN LEAF SHEATH DEVELOPMENT OF RICE -
MATSUKURA Chiaki, KAWAI Maki, TOYOFUKU Kyoko, BARRERO Robert A., UCHIMIYA Hirofumi, YAMAGUCHI Junji, Plant and cell physiology, 40, s15, s15, Mar. 1999
English

CHARACTERIZATION OF SLENDER RICE, A CONSTITUTIVE GIBBERELLIN RESPONSE MUTANT OF RICE
IKEDA Akira, KITANO Hidemi, FUTSUHARA Yuzo, YAMAGUCHI Junji, Plant and cell physiology, 39, S70, S70, May 1998
English

REGULATORY MECHANISMS OF SUGAR TRANSPORT -EXPRESSION OF SUGAR TRANSPORTERS USING ISOLATED RICE EMBRYOS-
TOYOFUKU Kyoko, SAITOH Toshikazu, MATSUKURA Chiaki, HIROSE Tatsuro, OHSUGI Ryu, YAMAGUCHI Junji, Plant and cell physiology, 39, S131, S131, May 1998
English

CHARACTERIZATION OF GASR1 GENE RELATED TO MODE OF ACTION OF AUXIN FOR ROOT MORPHOGENESIS IN RICE
IKEDA Akira, FUTSUHARA Yuzo, YAMAGUCHI Junji, Plant and cell physiology, 39, S42, S42, May 1998
English

Isoforms of α-amylase in rice seeds germinated in submerged soil and low temperature
HUANG Jirong, YAMAGUCHI Junji, AKITA Shigemi, Japanese Journal of Crop Science, 67, 94, 95, 02 Apr. 1998
The Crop Science Society of Japan, English

高等植物における細胞分裂と生理機能の制御機構の分子遺伝学的解析
安積 良隆, 鈴木 秀穂, 山口 淳二, 川上 直人, 年報, 97, 9, 13, Mar. 1998
生き物は成長するために細胞分裂し細胞増殖しなければならない。しかしそれも植物の場合にはその時期が適切でなければならない。移動能力のある動物の場合は受精と同時に発生を開始し、できるだけ速やかに成長することが生き残るためにもっとも大事なことといえる。しかし移動能力のない植物の場合は適切な時期に発生や成長をしなければ、枯死する運命が待ち受けている。成長に不利な時期を耐え、生き残るために、植物は発生や成長の時期を制御したり、種子を形成して休眠する。そして適当な時期が到来すると発芽し発生を開始する。生殖を行うために花を形成するのにも時期が大切である。さらに植物に特有な現象であるがセネッセンスと呼ばれる現象がある。これは環境がその植物の成長に不適切で個体を維持するのが困難であったり不利であったりする場合に、その個体の一部あるいは大部分の生理機能を低下させたり枯死させたりし、環境が回復するのを待つというものである。このように植物はその生活環の様々な場面で細胞分裂や生理機能の調節を行っている。我々は i) 種子の発芽の誘導機構、ii) 栄養成長から生殖成長への転換(花器官形成)の誘導機構、iii) 緑葉におけるセネッセンスの誘導機構の 3 つの現象に着目し分子遺伝学の手法を用いて解析を行う。, 神奈川大学, Japanese

Regulation of α-Amylase Gene Expression : -As a Tool to Study on Tranduction Mechanisms for Plant Hormonal-and Sugar-Signaling-
TOYOFUKU Kyoko, Shin-ichiro MITSUNAGA, YAMAGUCHI Jyunji, Chemical Regulation of Plants, 32, 2, 132, 143, 25 Dec. 1997
The Japanese Society for Chemical Regulation of Plants, Japanese

11 Transcriptional regulation of rice α-amylase gene, Ramy1A
Morita Akiyoshi, Toyofuku Kyoko, Perata Pierdomenico, Yamaguchi Junji, 植物化学調節学会研究発表記録集, 32, 27, 27, 28 Nov. 1997
The Japanese Society for Chemical Regulation of Plants, Japanese

12 Regulation of cell-wall extensibility by gibberellin: Comparative studies on visco-elastic parameters of creep extension among gibberellin-/auxin-induced elongation of leaf sheath/stem and GA-/acid-induced elongation of roots.
E. Tanimoto, R. Yamamoto, J. Yamaguchi, T. Matsukura, 植物化学調節学会研究発表記録集, 32, 29, 30, 28 Nov. 1997
The Japanese Society for Chemical Regulation of Plants, Japanese

10 Sugar repression of gibberellic acid-induced α-amylase genes in barly seeds. : possible crosstalk between hormone and sugar regulation of gene expression
Perata Pierdomenico, Matsukura Chiaki, Vernieri Paolo, Yamaguchi Junji, 植物化学調節学会研究発表記録集, 32, 25, 25, 28 Nov. 1997
The Japanese Society for Chemical Regulation of Plants, Japanese

78 The expression patterns of α- and β-amylase genes in germinating rice seeds under low temperature and hypoxia condition.
Yoon Byeong-Sung, Nemoto Keisuke, Yamaguchi Junji, Akita Shigemi, Japanese Journal of Crop Science, 66, 1, 156, 157, 02 Apr. 1997
The Crop Science Society of Japan, Japanese

SUGAR SENSING AND α-AMYLASE GENE REPRESSION IN RICE EMBRYOS
UEMURA Taka-aki, PERATA Pierdomenico, FUTSUHARA Yuzo, YAMAGUCHI Junji, Plant and cell physiology, 38, s137, Mar. 1997
English

1 Leaf sheath elongation stimulated by gibberellin application in rice
C. Matsukura, S. Itoh, A. Ikeda, Perata Pierdomenico, J. Yamaguchi, 植物化学調節学会研究発表記録集, 31, 13, 14, 04 Oct. 1996
The Japanese Society for Chemical Regulation of Plants, Japanese

ANALYSIS OF GIBBERELLIN-INDUCED ELONGATION OF LEAF SHEATH OF RICE
ITOH Shin-ichi, MATSUOKA Makoto, YAMAGUCHI Junji, Plant and cell physiology, 37, 144, 144, Mar. 1996
English

28 Difference in the Relationship between Seedling Emergence and Amylolytic Activity among Rice Cultivars.
Yoon Byeong-Sung, Yamaguchi Junji, Akita Shigemi, Japanese Journal of Crop Science, 65, 2, 55, 56, 1996
The Crop Science Society of Japan, Japanese

Polymorphism of Nuclear DNA from Various Rice Cultivars, as Detected by Staining of Gels after Pulsed-field Gel Electrophoresis
Hatano Shoji, Yamaguchi Junji, Tsutsumi Nobuhiro, Hirai Atsushi, Breeding science, 45, 2, 205, 209, 01 Jun. 1995
A new method was developed for preparation of highmolecularweight (HMW) DNA from rice using young or mature leaves as source material. Compared with the ori-ginal method, in which germ' tissues were used as source material (Hatano et al., 1992), the new method is simpler and faster because the procedure for isolation of germ tissues is no longer necessary. Moreover, even if a limited supply of seeds is expected, HMW DNA can be prepared from mature leaves derived from a smaller number of seeds. Therefore, the method is suitable for screening genomes of a number of varieties of rice for analysis by pulsedfield gel electrophoresis. HMW DNA from rice varieties that included nine Japonica cultivars, one Indica cultivar, and one strain of Oryza puuctata, a wild rice was prepared by the newly developed method. Each of the samples was digested with restriction enzymes and fragments were separated by pulsedfield gel electrophoresis. After staining of gels, a microfine banding pattern was visualized over a smeared background in each lane. Each pattern was specific for a specific variety of rice. Furthermore, although similarities were found among the Japonica cultivars tested, few common microbands were observed among the Indica cultivar, the strain of Oryza punctata and the group of Japonica cultivars tested. These observa-tions indicate that a high degree of polymorphism of nuc-lear DNA among rice varieties can be detected by simple staining of gels after pulsed-field gel electrophoresis., Japanese Society of Breeding, English

ANALYTICAL STUDIES ON STARCH BREAKDOWNIN CEREAL SEEDINGS - STARCH DEGRADING ENZYMES DURING ANOXICGERMINATION OF RICE, BARLEY AND WHEAT
YAMAGUCHI Junji, GUGLIELMINETTI Lorenzo, MASUI Hironori, ALPI Amedeo, PERATA Pierdomenico, Plant and cell physiology, 36, S20, Mar. 1995
English

ANALYSIS ON MODE OF ACTION OF GIBBERELLIN USING SPECIFIC INDEX, INDUCTION OF ALPHA-AMYLASE
S MITSUNAGA, J YAMAGUCHI, NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 67, 2, 156, 159, Feb. 1993
Japanese, Book review

Analysis on Mode of Action of Gibberellin Using Specific Index, Induction of α-Amylase
Shin-Ichiro Mitsunaga, Junji Yamaguchi, Nippon Nogeikagaku Kaishi, 67, 2, 156, 159, 1993
English

種子貯蔵デンプン分解系
山口 淳二, 植物細胞工学, 5, 184, 192, 1993
Japanese

ENZYMATIC MECHANISMS OF STARCH SYNTHESIS IN RIPENING RICE GRAINS .8. DISTINCT PROFILES OF ADP-SPECIFIC AND UDP-SPECIFIC SUCROSE SYNTHASES IN DEVELOPING RICE GRAINS
PY LIM, P PERATA, J POZUETAROMERO, T AKAZAWA, J YAMAGUCHI, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 56, 4, 695, 696, Apr. 1992
English, Introduction scientific journal

Germination-genes induced by gibberellins
J. Yamaguchi, S. Mitsunaga, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 37, 7, 1239, 1248, 1992
Japanese, Book review

発芽過程-ジベレリンによって発現される遺伝子群　　　　　　　　　　　　　　　
山口淳二, 光永伸一郎, 蛋白質核酸酵素, 37, 243, 252, 1992
Japanese

Introduction to the experiments of genetic engineering. XVIII. Regulatory mechanisms of gene expression in higher plants.
YAMAGUCHI Junji, AKAZAWA Takashi, RADIOISOTOPES, 38, 4, p229, 236, Apr. 1989
Japan Radioisotope Association, Japanese

2Ap02 イネ α-アミラーゼ遺伝子の転写調節機構 イネ形質転換系を用いたシス活性化因子の検索
山口 淳二, 梅村 賢司, 林 誠, 赤沢 堯, 日本植物生理学会年会およびシンポジウム : 講演要旨集, 29, 307, 307, 1989
日本植物生理学会, Japanese

1Dp-8 マイクロボディ変換機構の解析 : 組織化学的アプローチ
山口 淳二, 森 仁志, 西村 幹夫, 赤沢 尭, 横田 貞記, 日本植物生理学会年会とシンポジウム講演要旨集, 26, 127, 127, 1986
日本植物生理学会, Japanese

脂肪性種子子葉におけるマイクロボディ変換機構の解析　　　　　　　　　　　　　　　
山口 淳二, 化学と生物, 23, 491, 493, 1985
Japanese

1Ba-5 カタラーゼ生合成機構 : カタラーゼ前駆体の精製とその諸性質
山口 淳二, 西村 幹夫, 赤沢 堯, 日本植物生理学会年会とシンポジウム講演要旨集, 24, 74, 74, 1984
日本植物生理学会, Japanese

3Dp-1 マイクロボディ変換機構の解析 (4)カボチャ子葉におけるグリコール酸酸化酵素の___- ___-生合成
西村 幹夫, 山口 淳二, 赤沢 尭, 日本植物生理学会年会とシンポジウム講演要旨集, 23, 301, 301, 1983
日本植物生理学会, Japanese

3Dp-2 マイクロボディ変換機構の解析 (5)カボチャ子葉グリオキシゾームおよびパーオキシゾームカタラーゼのタンパク化学的比較
山口 淳二, 西村 幹夫, 赤沢 尭, 日本植物生理学会年会とシンポジウム講演要旨集, 23, 302, 302, 1983
日本植物生理学会, Japanese

3Ba-7 マイクロボディ変換機構の解析 (2)カボチャ子葉カタラーゼの精製と免疫化学的性質
山口 淳二, 西村 幹夫, 赤沢 堯, 日本植物生理学会年会およびシンポジウム : 講演要旨集, 22, 214, 214, 1982
日本植物生理学会, Japanese

Plant Proteomics: Methods in Molecular Biology, vol. 1072　　　　　　　　　　　　　　　
Sato T, Sako K, Yamaguchi J, Chapter 45 Assay for proteasome-dependent protein degradation and ubiquitinated proteins
Humana Press, 2013, 9781627036306, 655-663, [Contributor]

Frontiers in Agriculture Proteome Research　　　　　　　　　　　　　　　
Yasuda S, Maekawa S, Sato T, Yamaguchi J, Proteomics approach to study metabolic regulation mediated by ubiquitin-proteasome system in Arabidopsis
NARO Institute of Crop Science, 2012, 9784904633991, 39-44, [Contributor]

植物の百科事典　　　　　　　　　　　　　　　
山口淳二, 炭素代謝
朝倉書店, 2009, 9784254171372, 9, [Contributor]

Botanical encyclopedia　　　　　　　　　　　　　　　
Asakura, 2009

種子の科学とバイオテクノロジー　　　　　　　　　　　　　　　
佐藤長緒, 山口淳二, 種子デンプンの分解
学会出版センター, 2009, 9784762230592, 88-91, [Contributor]

バイオとナノの融合Ⅰ　　　　　　　　　　　　　　　
園田 裕, 佐藤長緒, 山崎直子, 佐古香織, 池田 亮, 山口淳二, プロテアソームを介した細胞制御-高等植物の細胞サイズ制御を中心として
北海道大学出版会, 2007, 239-255, [Contributor]

朝倉植物生理学講座第２巻代謝　　　　　　　　　　　　　　　
武田泰斗, 山口淳二, デンプンの合成と分解
朝倉書店, 2001, 4254176562, 94-102, [Contributor]

新農学大事典　　　　　　　　　　　　　　　
山口淳二, 松倉千昭, 発芽
養賢堂, 2001, [Contributor]

植物ホルモンと細胞の形　　　　　　　　　　　　　　　
山口淳二, 松倉千昭, α-アミラーゼの誘導
学会出版センター, 1998, 4762218707, 43-52, [Contributor]

植物化学調節実験マニュアル　　　　　　　　　　　　　　　
山口 淳二, アリューロン層細胞を用いたα-アミラーゼ誘導検定法
全国農村教育協会, 1997, 4881370634, 2-7, [Contributor]

種子のバイオサイエンス　　　　　　　　　　　　　　　
山口 淳二, 種子デンプン（炭水化物）の合成と分解
学会出版センター, 1995, 476228789X, 87-93, [Contributor]

酵素実験法（１）　　　　　　　　　　　　　　　
山口淳二, 赤沢堯, 植物酵素の精製
1993, 4567243609, 80-87, [Contributor]

遺伝子工学実験　　　　　　　　　　　　　　　
山口 淳二, 植物細胞における遺伝子クローンの発現
日本アイソトープ協会, 1991, 143-150, [Contributor]

新生化学実験講座 Vol 1　　　　　　　　　　　　　　　
山口淳二, 赤沢堯, 植物組織と抽出法
東京化学同人, 1990, 4807910477, 20-27, Japanese, [Joint work]

栄養シグナルによる膜交通制御と植物成長最適化機構の解明
科学研究費助成事業 基盤研究(B)
01 Apr. 2021 - 31 Mar. 2024
山口 淳二, 佐藤 長緒, 高木 純平
日本学術振興会, 基盤研究(B), 北海道大学, 21H02150

Studies on pathogen recognition and functional expression for plant immunity mediated by ubiquitin modification
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2018 - 31 Mar. 2021
Yamaguchi Junji
In this study, we focused on the function of ATL31, a membrane-localized ubiquitin ligase conserved in plants, and investigated the signal transduction mechanism regulating pathogen resistance in plants. Our biochemical analyzes demonstrated that ATL31’s ubiquitin ligase activity was changed in response to MAMPs. We also found the specific ubiquitin chain type signals are involved in the ATL31-mediating pathogen attack signals in plan cells.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 18H02162

植物膜タンパク質のユビキチン修飾と機能発現制御機構の解明
科学研究費補助金(新学術領域研究(研究領域提案型))
2015 - 2016
山口 淳二
本研究では「植物膜タンパク質のユビキチン修飾と機能発現制御機構の解明」を目指し，3研究課題に取組んでいる。H28年度は以下のような成果を得た。
植物に特有の膜局在型ユビキチンリガーゼATL遺伝子族は，標的となる膜タンパク質をユビキチン化することで，膜タンパク質の安定化，細胞内局在等の制御に関与する。また，その性質から，細胞膜を介した環境シグナルの受容・伝達制御に深く関わる。申請者は，基幹代謝制御に関与するATL31の発見以来，一貫してATL族の機能解明を進めてきた。本申請では，ATL31による細胞膜上の環境シグナル受容と伝達制御機構の全容解明を目指す。また， ATL31の膜局在性に着目し，細胞の内と外の情報受容・伝達制御にATL31がどのように関与するかについて検討した。
ATL31はユビキチンリガーゼ活性に依存的してC/N応答を制御するが，これまでの解析でATL31の細胞内局在性がユビキチンリガーゼ活性依存的に変化することを発見し，ATL31の細胞内局在性がその機能と密接に関係することが示唆されていた。ATL31のユビキチン化標的を調べ得るためIP-MS解析による相互作用因子の探索を行ったところ，SNAREタンパク質が複数同定された。SNAREタンパク質群はSANRE複合体を形成することで膜融合を促進する膜交通制御の鍵因子である。その中でもSYP61は重要で，受容体やトランスポーター，細胞壁合成装置などの様々なタンパク質の小胞を介した輸送制御の中心を担っている。興味深いことに，ATL31はこのSYP61と相互作用し，ユビキチンリガーゼ活性依存的にSYP61の細胞内局在性を変化させることを発見した。これらの結果から，ATL31がSYP61を介して膜交通に作用することでC/N応答を制御する可能性が示唆された。
文部科学省, 新学術領域研究(研究領域提案型), 北海道大学, Principal investigator, Competitive research funding, 15H01167

Regulation of environmental signalling mediated by membrane-localized plant ubiquitin ligase ATL31
Grants-in-Aid for Scientific Research
2014 - 2016
YAMAGUCHI Junji, SAKO Kaori, MAEKAWA Shugo
Arabidopsis ATL31 is a membrane-localized ubiquitin ligase that functions in not only C/N-nutrient response but defense response. We identified SYP121 as a novel ATL31 interactor. SYP121 is an essential factor for plant resistance against powdery mildew fungus and positively regulates the formation of cell wall apposition, called papillae, at the fungal entry site. We finally indicated that ATL31 plays an important role in basal immunity by papilla formation through association with SYP121. The molecular basis of C/N-nutrient signaling also remains unclear. We identified three calcineurin B-like (CBL)-interacting protein kinases (CIPKs) as key regulators of the C/N-nutrient response. Further analyses showed that these CIPKs are required for ATL31 phosphorylation and stabilization, which mediates the degradation of 14-3-3 in response to C/N conditions in Arabidopsis. These findings provide new insights into C/N-nutrient signaling mediated by protein phosphorylation.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 26292188

Flowering regulation in response to C/N nutrient availability
Grants-in-Aid for Scientific Research
2014 - 2015
YAMAGUCHI Junji
Carbon (C) and nitrogen (N) are essential elements for metabolism and relative balance of the availability, called as C/N balance, which must be tightly coordinated for the optimal growth in plants. Especially, phase transition such as flowering is suggested to be affected by C/N balance. Although there are a lot of findings of flowering regulation system in response to environmental cues, the relationship between flowering and C/N nutrient status is still unclear. To elucidate the molecular mechanism of C/N-inducible flowering, we performed C/N-responsible flowering analysis using an atmospheric CO2 manipulation system combined with hydroponic culture to regulate N levels. We found that high C/low N condition promotes the flowering of Arabidopsis plant under long-day condition. In this meeting, we will present analyses of gene expression, metabolites, proteome and discuss about flowering regulation system by C/N-nutrient availability.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 26660046

ユビキチン修飾による膜交通と葉緑体形成制御機構の解明
科学研究費補助金(新学術領域研究(研究領域提案型))
2013 - 2014
山口 淳二
本研究では「ユビキチン修飾による膜交通と葉緑体形成制御機構の解明」を目指し，3研究課題に取組んでいる。H26年度は以下のような成果を得た。
本年度の研究実施計画
計画２）ユビキチン修飾による膜交通-植物免疫制御機構の解析： ATL31と相互作用するSNAREタンパク質SYP121は，植物の病害抵抗性と深いかかわりを持つ。糸状菌であるうどん粉病菌を植物に感染させると，SYP121欠損変異植物syp121-1では野生型（WT）と較べて抵抗性が劇的に減少するのに対し，ATL31過剰発現植物ATL31oxでは，抵抗性が上昇する。この抵抗性には，パピラという細胞壁の肥厚化が関与している。パピラは，カロース等の細胞壁成分がゴルジ体からの小胞輸送により感染箇所に分泌されることで形成される。解析の結果，i) 膜局在型ATL31によるSNAREタンパク質のユビキチン化→ii) パピラ形成部位の決定→iii) 細胞壁の肥厚化（パピラ形成）→iv) 病原体抵抗性の亢進，という一連の膜交通-植物免疫シグナル伝達制御反応に関する仮説を証明し，論文発表した（Plant Physiol. 2014）。
計画３）ユビキチン修飾による葉緑体形成制御機構の解析: 植物プロテアソームと相互作用するタンパク質のプロテオーム解析により，複数の葉緑体局体タンパク質が同定された。詳細な研究解析の結果，i) プロテアソームサブユニットRPT2aは，複数の葉緑体タンパク質移行トランジットペプチド（以下cTP）と相互作用すること，また，ii) LTA2のような葉緑体タンパク質は，ユビキチンリガーゼAtCHIPと相互作用すること，等を証明し，論文発表した（J. Proteome Res, 2014）。これにより，ユビキチン修飾による新たな制御機構-タンパク質のオルガネラ移行制御-の可能性を示唆した。
文部科学省, 新学術領域研究(研究領域提案型), 北海道大学, Principal investigator, Competitive research funding, 25112501

ユビキチンカスケードが関わるC/Nバランスと高CO2応答の全容解明
科学研究費補助金(新学術領域研究(研究領域提案型))
2012 - 2012
山口 淳二
本研究計画では，高CO2条件下におけるC/N応答制御の分子ネットワーク全容解明を目指している。今年度は，計画1），2）のタンパク質相互作用に着目したC/Nシグナル伝達の分子基盤解析に加えて，3）CO2/Nによる植物の成長制御に関しても「花成」および「老化」に着目した生理学的解析を進めた。
1)詳細な生化学的解析と形質転換植物の解析を実施し，ATL31と14-3-3との相互作用と機能にリン酸化が必須であることを証明し，また，そのキナーゼの同定を進めた[J. Bio. Chem. 2014]。
2)ATL31がSNAREタンパク質SYP121との相互作用によりパピラ形成を制御し，うどん粉病耐性に関与することを証明した[Plant Physiol. 2014]。
3) ATL31と14-3-3等関連遺伝子導入植物を用いて，高CO2条件下で応答実験を実施した。高CO2（700 ppm）条件下では，野生型において老化の促進が観察されるが，ATL31過剰発現体ではその抑制が，またATL31KO変異体ではその亢進が観察できた。これにより，ATL31がC/N応答だけでなく，CO2/N応答にも関与することを証明した。また，その老化に関与する転写因子の同定に成功した[Plant Cell Physiol. 2014; Plant Signal. Behav. 2014]。
文部科学省, 新学術領域研究(研究領域提案型), 北海道大学, Principal investigator, Competitive research funding, 24114701

Studies for biosynthesis, transport, secreation of glycoprotein in higher plant.
Grants-in-Aid for Scientific Research
1987 - 1988
AKAZAWA Takashi, KOBAYASHI Hirokazu, TAKABE Tetsuko
Since rice seed -amylase is shown to be a typical Asn-N-linked secretory glycoprotein, the excised rice seedlings have been proved to be a feasible material to study the mechanism of the biosynthesis as well as the intracellular transport of the secretory protein in plant cells. It has been shown that -amylase molecules bearing both high mannose-type and complex-type oligosaccharide chains are secreted from the rice scutellar tissues, and a hypothetical mechanism concerning the stryctural modification of the oligosaccharide moiety linked the -amylase polypeptide chain in the golgi compartment has been proposed.
In order to further study the detailed enzymic machineries for rice -amylase biosynthesis, we have been currently employing the liquid suspension-cultured cells. Using this suspension-cultured cells, we got several data as follows.
1. Oligosaccharide structure in secreted -amylase is (GlcNAc)_2Man_3GlcNAc_2 or GlcNAcMan_3GlcNAc_2. These structures are plant specific complex type oligosaccharide.
2. Complex type oligosaccharide chain in secreted -amylase is produced from the precursor containing high-mannose type oligosaccharide chain.
3. When the oligosaccharide modification is inhibited by some environmental factors, secreted -amylase has high-mannose type oligosaccharide chain.
Japan Society for the Promotion of Science, Grant-in-Aid for General Scientific Research (B), Nagoya University, 62470119