芳村 毅 (ヨシムラ タケシ)
水産科学研究院 海洋生物資源科学部門 海洋環境科学分野 | 准教授 |
Last Updated :2024/12/06
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研究者番号
- 20371536
Researcher ID
- M-1778-2017
J-Global ID
■研究活動情報
論文
- Kelp dissolved organic carbon release is seasonal and annually enhanced during senescence
Andrew Kalani Carlson, Takeshi Yoshimura, Isao Kudo
Journal of Phycology, 2024年, [査読有り]
研究論文(学術雑誌), Macroalgae influence local and global biogeochemical cycles through their production of dissolved organic carbon (DOC). Yet, data remain scarce and annualized estimates are typically based on high growth periods without considering seasonal variability. Although the mechanisms of active exudation and passive leakage need clarifying, ecophysiological stress is known to enhance DOC release. Therefore, DOC leakage from seasonally senescent macroalgae may be overlooked. This study focuses on the annual kelp Saccharina japonica var. religiosa (class Phaeophyceae) from Oshoro Bay, Hokkaido, Japan. Three years (2020–2022) of seasonal data were collected and analyzed, with least squares mean DOC release rates established for kelp (n = 88) across 16 incubation experiments (t ≥ 4 d, DOC samples ≥1 · d−1) under different photosynthetically active radiation (PAR) treatments (200, 400, 1200, or 1500 μmol photons · m−2 · s−1). Differences in PAR, dry weight biomass (g DW), sea surface temperature, or salinity could not explain DOC release-rate variability, which was high between individual kelp. Instead, there were significant intra-annual differences, with mean DOC release rates (mg C · g−1 DW · d−1 ± standard error between n kelp) higher during the autumn “late decay” period (0.71 ± 0.10, n = 27) compared to the winter “early growth” period (0.14 ± 0.025, n = 10) and summer “early decay” period (0.25 ± 0.050, n = 24). This relationship between seasonal senescence and macroalgal DOC release is further evidence that long-term, place-based studies of DOC dynamics are essential and that global extrapolations are premature. - Contribution of ammonium oxidizing archaea and bacteria to intensive nitrification during summer in Mutsu Bay, Japan
Akari Aizawa, Yuka Watanabe, Kaori Hashioka, Aya Kadoya, Satoru Suzuki, Takeshi Yoshimura, Isao Kudo
Regional Studies in Marine Science, 63, 2023年10月, [査読有り]
研究論文(学術雑誌), Nitrification, in which ammonium is oxidized to nitrate via nitrite, is a regeneration process of bioavailable nitrogen. Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) carry out the ammonium oxidation. Here we measured the ammonium oxidation rates (AORs), distinguishing those of AOA (VA) and AOB (VB), for two years in Mutsu Bay, a subarctic semi-enclosed bay. Nitrate and ammonium were depleted in the upper layer in summer, but a high concentration of nitrate was observed at the bottom (below 40 m), of up to 7.8 μM with a strong vertical gradient within several meters of the seafloor. A High AOR of up to 0.17 μM d−1 were observed in the bottom waters. The AOA were responsible for most of the total AOR, but the VB exceeded the VA just above the seafloor (0.3 m) in September. Removal of the suspended solids (SS) did not change the AOA's oxidation activity but eliminated the AOB's. This finding suggested that AOA were free-living whereas AOB were attached to the SS's surface. It is also suggested that the sediment was this SS's source. When ammonia mono-oxygenase, amoA, of AOA and AOB, was quantified, the copy number of AOA-amoA was an order of magnitude higher than that of AOB-amoA throughout the study period. A significant correlation (p < 0.05) was found between the AOB-amoA copy number and VB whereas no correlation was found between the AOA-amoA copy number and VA suggesting the AOA-amoA copy number did not always represent active cells. Ammonia oxidation was occurring near the bottom waters of Mutsu Bay during the summer, and it was accompanied by a dynamic balance reflecting the difference in the AOA's and AOB's lifestyles, free-living and particle-associated, respectively. - 汽水湖尾駮沼の塩分再現性から得られた沼・海洋間の水交換
坪野 考樹, 津旨 大輔, 三角 和弘, 芳村 毅
土木学会論文集B3(海洋開発), 78, 2, I_625, I_630, Japan Society of Civil Engineers, 2022年05月, [査読有り]
日本語, 研究論文(学術雑誌) - 海水標準物質の現状と将来展望-栄養塩CRMをはじめとして-
村田昌彦, 青山道夫, 青山道夫, チョン千香子, 三浦勉, 藤井武史, 光田均, 北尾隆, 笹野大輔, 中野俊也, 永井直樹, 児玉武稔, 葛西広海, 清本容子, 瀬藤聡, 小埜恒夫, 横川真一朗, 有井康博, 曽根知実, 石川賀子, 芳村毅, 内田裕, 田中辰弥, 粥川洋平, 脇田昌英
海の研究, 29, 5, 2020年, [査読有り]
研究論文(学術雑誌) - Mechanisms of radiocesium depuration in Sebastes cheni derived by simulation analysis of measured 137Cs concentrations off southern Fukushima 2014–2016
Takashi Ishimaru, Yutaka Tateda, Daisuke Tsumune, Michio Aoyama, Yasunori Hamajima, Nobue Kasamatsu, Manabu Yamada, Takeshi Yoshimura, Takuji Mizuno, Jota Kanda
Journal of Environmental Radioactivity, 203, 200, 209, Elsevier BV, 2019年07月, [査読有り], [国際誌]
英語, 研究論文(学術雑誌), The cesium depuration mechanisms were studied in Japanese rockfish Sebastes cheni off Fukushima, in which the radiocesium level remains higher than in other teleost. Samples were collected approximately 5 km south from the nuclear power plant during 2014-2016, and the 137Cs concentrations in fish, stomach content and prey species were measured. The stable cesium content in fish was also analyzed and compared with fish age which was determined by annual ring analysis in otoliths. The 137Cs concentrations in the dominant prey species, mysids and brown shrimp, were several Bq kg-w.w.-1; indicating that transfer via the food chain was substantial compared to that from seawater during the study period. The 137Cs concentrations in S. cheni decreased from 2014 to 2016 due to the metabolic excretion and the rate of decrease in its diet. Biokinetic model analyses confirmed the slower turnover of stable cesium in S. cheni, represented as a biological half-life (Tb1/2) of 140-215 d, and was associated with stable Cs levels in food of 5-7 ng g-w.w.-1. The 137Cs levels in S. cheni were also simulated, which showed that the 137Cs depuration in fish exposed to the initial contaminated plume in 2011 resulted from slower metabolic excretion, while the 137Cs levels in fish born after 2012 could be regarded as equilibrated with the environmental levels of 137Cs. Furthermore, the simulation results suggest that 137Cs depuration in S. cheni population was also caused by the alternation of generation, which can be substantial by the addition of new year class population hatched after 2012 that were not contaminated by the initial contaminated plume from the 2011 accident. - Sunlight caused interference in outdoor N, N-diethyl-p-phenylenediamine colorimetric measurement for residual chlorine and the solution for on-site work
Takeshi Yoshimura
Ecotoxicology and Environmental Safety, 169, 640, 644, 2019年03月01日, [査読有り]
英語, 研究論文(学術雑誌), Chlorination is the most common method to control water qualities, in some case on-site outdoor measurements are required to measure easily-decaying residual chlorine concentration appropriately without delay. In this study sunlight-induced unexpected colour development (UCD) of N, N-diethyl-p-phenylenediamine (DPD) colorimetric measurement was studied under several sun exposure conditions. The colour development level was evaluated with reference to chlorine concentration (mg/L) and relationships between colour development rate (mg/L min) and intensities of solar were investigated. UCD was found to be related to both exposure intensity and time. By means of exposure experiment under specific wavelength of ultraviolet (UV), it was confirmed that both middle and short wavelength of UV radiation being responsible for such an unexpected measurement. Consequently, a simple device was designed using three commercially available anti-UV films, one of which could effectively prevent the UCD from direct sun exposure. - Impact of CO2 on the elemental composition of the particulate and dissolved organic matter of marine diatoms emerged after nitrate depletion
Koji Sugie, Takeshi Yoshimura, Masahide Wakita
Limnology and Oceanography, Wiley, 2018年09月, [査読有り]
研究論文(学術雑誌) - Dynamics of particulate and dissolved organic and inorganic phosphorus during the peak and declining phase of an iron-induced phytoplankton bloom in the eastern subarctic Pacific
Takeshi Yoshimura, Jun Nishioka, Hiroshi Ogawa, Atsushi Tsuda
JOURNAL OF MARINE SYSTEMS, 177, 1, 7, ELSEVIER SCIENCE BV, 2018年01月, [査読有り]
英語, 研究論文(学術雑誌), Phosphorus (P) is an essential element for all organisms and thus the P cycle plays a key role in determining the dynamics of lower trophic levels in marine ecosystems. P in seawater occurs conceptually in particulate and dissolved organic and inorganic (POP, PIP, DOP, and DIP, respectively) pools and clarification of the dynamics in these P pools is the basis to assess the biogeochemical cycle of P. Despite its importance, behaviors of each P pool with phytoplankton dynamics have not been fully examined. We measured the four operationally defined P pools (POPop, PIPop, DOPop, and SRP) during an iron-induced phytoplankton bloom (as part of the subarctic ecosystem response to iron enrichment study (SERIES)) in the eastern subarctic Pacific in summer 2002. During our observations of the iron-enriched patch from day 15 to day 26 after the iron infusion, chlorophyll-alpha concentration in the surface layer decreased from 6.3 to 1.2 mu g L-1, indicating the peak through decline phase of the phytoplankton bloom. At the bloom peak, P was partitioned into POPop, PIPop, and DOPop in proportions of 60, 27, and 13%, respectively. While chlorophyll-alpha and POPop showed similar temporal variations during the declining phase, PIPop showed a different peak timing with a 2 day delay compared to POPop, resulting in a rapid change in the relative proportion of PIPop to total particulate P (TPP = POPop + PIPop) at the peak (25%) and during the declining phase of the bloom (50%). A part of POPop was replaced by PIPop just after slowing down of phytoplankton growth. This process may have a significant role in the subsequent regeneration of P. We conclude that measurement of TPP alone is insufficient to show the interaction between P and phytoplankton dynamics and fractionation of TPP into POPop and PIPop provides useful insights to clarify the biogeochemical cycle of P. - Response of Spring Diatoms to CO2 Availability in the Western North Pacific as Determined by Next-Generation Sequencing
Hisashi Endo, Koji Sugie, Takeshi Yoshimura, Koji Suzuki
PLOS ONE, 11, 4, PUBLIC LIBRARY SCIENCE, 2016年04月, [査読有り]
英語, 研究論文(学術雑誌), Next-generation sequencing (NGS) technologies have enabled us to determine phytoplankton community compositions at high resolution. However, few studies have adopted this approach to assess the responses of natural phytoplankton communities to environmental change. Here, we report the impact of different CO2 levels on spring diatoms in the Oyashio region of the western North Pacific as estimated by NGS of the diatom-specific rbcL gene (DNA), which encodes the large subunit of RubisCO. We also examined the abundance and composition of rbcL transcripts (cDNA) in diatoms to assess their physiological responses to changing CO2 levels. A short-term (3-day) incubation experiment was carried out on-deck using surface Oyashio waters under different pCO(2) levels (180, 350, 750, and 1000 mu atm) in May 2011. During the incubation, the transcript abundance of the diatom-specific rbcL gene decreased with an increase in seawater pCO(2) levels. These results suggest that CO2 fixation capacity of diatoms decreased rapidly under elevated CO2 levels. In the high CO2 treatments (750 and 1000 mu atm), diversity of diatom-specific rbcL gene and its transcripts decreased relative to the control treatment (350 mu atm), as well as contributions of Chaetocerataceae, Thalassiosiraceae, and Fragilariaceae to the total population, but the contributions of Bacillariaceae increased. In the low CO2 treatment, contributions of Bacillariaceae also increased together with other eukaryotes. These suggest that changes in CO2 levels can alter the community composition of spring diatoms in the Oyashio region. Overall, the NGS technology provided us a deeper understanding of the response of diatoms to changes in CO2 levels in terms of their community composition, diversity, and photosynthetic physiology. - Effects of high CO2 levels on the ecophysiology of the diatom Thalassiosira weissflogii differ depending on the iron nutritional status
Koji Sugie, Takeshi Yoshimura
ICES JOURNAL OF MARINE SCIENCE, 73, 3, 680, 692, OXFORD UNIV PRESS, 2016年02月, [査読有り]
英語, 研究論文(学術雑誌), Iron availability in seawater, namely the concentration of dissolved inorganic iron ([Fe']), is affected by changes in pH. Such changes in the availability of iron should be taken into account when investigating the effects of ocean acidification on phytoplankton ecophysiology because iron plays a key role in phytoplankton metabolism. However, changes in iron availability in response to changes in ocean acidity are difficult to quantify specifically using natural seawater because these factors change simultaneously. In the present study, the availability of iron and carbonate chemistry were manipulated individually and simultaneously in the laboratory to examine the effect of each factor on phytoplankton ecophysiology. The effects of various pCO(2) conditions (similar to 390, similar to 600, and similar to 800 mu atm) on the growth, cell size, and elemental stoichiometry (carbon [C], nitrogen [N], phosphorus [P], and silicon [Si]) of the diatom Thalassiosira weissflogii under high iron ([Fe'] = similar to 240 pmol l(-1)) and low iron ([Fe'] = similar to 24 pmol l(-1)) conditions were investigated. Cell volume decreased with increasing pCO(2), whereas intracellular C, N, and P concentrations increased with increasing pCO(2) only under high iron conditions. Si:C, Si:N, and Si:P ratios decreased with increasing pCO(2). It reflects higher production of net C, N, and P with no corresponding change in net Si production under high pCO(2) and high iron conditions. In contrast, significant linear relationships between measured parameters and pCO(2) were rarely detected under low iron conditions. We conclude that the increasing CO2 levels could affect on the biogeochemical cycling of bioelements selectively under the iron-replete conditions in the coastal ecosystems. - DPD法での海水中の残留塩素測定における珪藻Coscinodiscus wailesiiの存在による計測妨害
芳村 毅, 柳川 敏治, 藤原 淳, 村井 圭次, 野方 靖行, 古田 岳志, 小林 卓也
Sessile Organisms, 33, 2, 38, 43, 日本付着生物学会, 2016年, [査読有り]
日本語, Chlorination of seawater is a common practice to prevent biofouling on maritime structures. To enable control and reduce the environmental impacts of chlorine we measure the residual chlorine in wastewaters. In routine measurements of residual chlorine using N,N-diethyl-p-phenylenediamine (DPD), we found significant color development by DPD in non-chlorinated waters when large-sized phytoplankton appeared in high abundance. Through screening 27 microalgal isolates in laboratory culture, the diatom Coscinodiscus wailesii was shown to be the only species that causes the DPD color development in the procedure for total residual chlorine, although not for free residual chlorine, despite no chlorine additions to the culture medium. The result was also confirmed in field samples. Since two other species of genus Coscinodiscus did not show a similar result, this is considered to be highly species-specific. The color development was prevented by removing the C. wailesii cells using a 100 µm mesh cell strainer. Coscinodiscus wailesii did not react with 3,3′-dimethylbenzidine (o-tolidine), an alternative reagent to measure residual chlorine. We conclude that C. wailesii produces some materials which can oxidize DPD to produce the red color, and C. wailesii blooms in the field interfere with measurements of residual chlorine concentrations in natural and waste seawater. - Primary productivity and nitrogen assimilation with identifying the contribution of urea in Funka Bay, Japan
Isao Kudo, Takatsugu Hisatoku, Takeshi Yoshimura, Yoshiaki Maita
ESTUARINE COASTAL AND SHELF SCIENCE, 158, 12, 19, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2015年06月, [査読有り]
英語, 研究論文(学術雑誌), Primary production is supported by utilization of several forms of nitrogen (N), such as nitrate, ammonium, and urea. Nevertheless, only few studies have measured the concentration and uptake of urea despite its importance as a nitrogenous nutrient for phytoplankton. We measured primary productivity monthly at four depths within the euphotic zone using a clean technique and the C-13 method by a 24 h in situ mooring incubation over a year in Funka Bay, a subarctic coastal area in Japan, to make better updated estimates (re-evaluation) of annual primary production. Nitrogenous (N) nutrient assimilation rates (nitrate, ammonium and urea) were also measured to elucidate the relative contributions of these nutrients to autotrophic production and to distinguish between new and regenerated production. The estimated annual primary production was 164 g C m(-2), which was 40-60% higher than the previously reported values in the bay. Use of a clean technique and more frequent measurement during the spring bloom may have contributed to the higher rates. The production during the spring bloom was 56.5 g C m(-2), accounting for 35% of the annual production. The maximum daily productivity occurred in the bloom at 1.4 g C m(-2) d(-1), which is one of the highest values among the world embayments. The annual primary production in the bay was classified as mesotrophic state based on the classification by Cloern et al. (2014). The assimilation rate of nitrate was maximal at 54 nmol N L-1 h(-1) during the bloom. During the post-bloom periods with nitrate depleted conditions, assimilation rates of ammonium and urea increased and accounted for up to 85% of the total N assimilation. The assimilation rate of urea was almost comparable to that of ammonium throughout the year. Taking urea into account, the f-ratio ranged from 0.15 under the nitrate-depleted conditions to 0.8 under the spring bloom conditions. These ratios were overestimated by 50% and 10%, respectively, if urea uptake was eliminated. We provide a valuable data for the primary production dataset in the world's ecosystems, and show that urea plays an important role in supporting regenerated production during late spring and summer. (C) 2015 Elsevier Ltd. All rights reserved. - Effects of CO2 and iron availability on rbcL gene expression in Bering Sea diatoms
H. Endo, K. Sugie, T. Yoshimura, K. Suzuki
BIOGEOSCIENCES, 12, 7, 2247, 2259, COPERNICUS GESELLSCHAFT MBH, 2015年, [査読有り]
英語, 研究論文(学術雑誌), Iron (Fe) can limit phytoplankton productivity in approximately 40% of the global ocean, including in high-nutrient, low-chlorophyll (HNLC) waters. However, there is little information available on the impact of CO2-induced seawater acidification on natural phytoplankton assemblages in HNLC regions. We therefore conducted an on-deck experiment manipulating CO2 and Fe using Fe-deficient Bering Sea water during the summer of 2009. The concentrations of CO2 in the incubation bottles were set at 380 and 600 ppm in the non-Fe-added (control) bottles and 180, 380, 600, and 1000 ppm in the Fe-added bottles. The phytoplankton assemblages were primarily composed of diatoms followed by haptophytes in all incubation bottles as estimated by pigment signatures throughout the 5-day (control) or 6-day (Fe-added treatment) incubation period. At the end of incubation, the relative contribution of diatoms to chlorophyll a biomass was significantly higher in the 380 ppm CO2 treatment than in the 600 ppm treatment in the controls, whereas minimal changes were found in the Fe-added treatments. These results indicate that, under Fe-deficient conditions, the growth of diatoms could be negatively affected by the increase in CO2 availability. To further support this finding, we estimated the expression and phylogeny of rbcL (which encodes the large subunit of RuBisCO) mRNA in diatoms by quantitative reverse transcription polymerase chain reaction (PCR) and clone library techniques, respectively. Interestingly, regardless of Fe availability, the transcript abundance of rbcL decreased in the high CO2 treatments (600 and 1000 ppm). The present study suggests that the projected future increase in seawater pCO(2) could reduce the RuBisCO transcription of diatoms, resulting in a decrease in primary productivity and a shift in the food web structure of the Bering Sea. - Organic matter production response to CO2 increase in open subarctic plankton communities: Comparison of six microcosm experiments under iron-limited and -enriched bloom conditions
Takeshi Yoshimura, Koji Sugie, Hisashi Endo, Koji Suzuki, Jun Nishioka, Tsuneo Ono
DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS, 94, 1, 14, PERGAMON-ELSEVIER SCIENCE LTD, 2014年12月, [査読有り]
英語, 研究論文(学術雑誌), Increase in seawater pCO(2) and the corresponding decrease in pH caused by the increasing atmospheric CO2 concentration (i.e., ocean acidification) may affect organic matter production by phytoplankton communities. Organic matter production forms the basis of marine food webs and plays a crucial role in oceanic CO2 uptake through the biological carbon pump, and hence will potentially affect future marine ecosystem dynamics. However, responses of organic matter production in open ocean plankton ecosystems to CO2 increase have not been fully examined. We conducted on-deck microcosm experiments using high nutrient, low chlorophyll (HNLC) waters in the western subarctic Pacific and oceanic Bering Sea basin in summer 2008 and 2009, respectively, to examine the impacts of elevated CO2 on particulate and dissolved organic matter (i.e., POM and DOM, respectively) production. Iron deficient natural plankton communities were incubated for 7-14 days under multiple CO2 levels with and without iron enrichments (hereafter +Fe and -Fe treatments, respectively). By combining with our previous experiments at two sites, we created a comprehensive dataset on responses of organic matter production to CO2 increase during macronutrient replete conditions in HNLC waters. Significant differences in net particulate organic carbon production among CO2 treatments were observed only in the -Fe treatments, whereas that in net dissolved organic carbon production were mainly observed in the +Fe treatments, suggesting that CO2 may affect different processes depending on the Fe nutritional status. However, impacts of CO2 were not consistent among experiments and were much smaller than the consistent positive effects of Fe enrichment. In contrast, no significant differences among the CO2 treatments were observed for organic carbon partitioning into POM and DOM, and carbon to nitrogen ratio of net produced POM. We conclude that CO2 does not play a primary role, but could have secondary effects on controlling the organic matter production under macronutrient replete conditions in HNLC waters. On the other hand, in a nutrient-depleted, declining phase of the phytoplankton bloom induced by Fe enrichment, carbon overconsumption was found in an experiment with elevated CO2 conditions suggesting that CO2 impacts might become more significant in such environments. (C) 2014 Elsevier Ltd. All rights reserved. - Dissolved organic phosphorus production and decomposition during open ocean diatom blooms in the subarctic Pacific
Takeshi Yoshimura, Jun Nishioka, Hiroshi Ogawa, Kenshi Kuma, Hiroaki Saito, Atsushi Tsuda
MARINE CHEMISTRY, 165, 46, 54, ELSEVIER SCIENCE BV, 2014年10月, [査読有り]
英語, 研究論文(学術雑誌), A significant part of phosphorus (P) in seawater is found in the dissolved organic matter (DOM) fraction as DOP, which plays a key role in the marine biogeochemical cycle of P. The DOM pool size changes with biological activity, but DOP production and decomposition processes, unlike carbon (C) and nitrogen, have been only infrequently studied during phytoplankton blooms when rapid production and accumulation of organic matter occurs. We observed the DOP dynamics during two phytoplankton blooms dominated by centric diatoms, the first induced by an in situ mesoscale iron enrichment experiment in the western subarctic Pacific in summer 2001 (SEEDS) and the second that occurred naturally in spring 2003 in the Oyashio region. DOP concentration increased with the buildup of phytoplankton biomass with DOP/chlorophyll-a production ratios (mol/g) of 0.0027 +/- 0.0004 and 0.0044 +/- 0.0010 for the SEEDS and Oyashio blooms, respectively. During the SEEDS and Oyashio blooms the amount of net DOP production corresponded to (4.9 +/- 0.7) % and (4.5 +/- 0.6) % of the consumed soluble reactive P, and (5.5 +/- 0.8) % and (13 +/- 3) % of the newly accumulated organic P was partitioned into DOP, respectively. Seawater culture bottle experiments showed that newly produced DOP during the bloom development was decomposed by free living bacteria over a time scale of a month even under soluble reactive P available conditions. C:P for the decomposed DOM (molar ratio of 78-88) showed a similar value to in situ net produced DOM (66) and POM (83) but much lower than that for the bulk DOM (395-706), suggesting that the composition of the freshly produced DOM with high lability differs significantly from the bulk DOM. (C) 2014 Elsevier B.V. All rights reserved. - Effects of CO2 and iron availability on rbcL gene expression in Bering Sea diatoms
H. Endo, K. Sugie, T. Yoshimura, K. Suzuki
Biogeosciences Discussions, 11, 12, 18105, Copernicus {GmbH}, 2014年 - Impacts of elevated CO2 on particulate and dissolved organic matter production: microcosm experiments using iron-deficient plankton communities in open subarctic waters
Takeshi Yoshimura, Koji Suzuki, Hiroshi Kiyosawa, Tsuneo Ono, Hiroshi Hattori, Kenshi Kuma, Jun Nishioka
JOURNAL OF OCEANOGRAPHY, 69, 5, 601, 618, SPRINGER, 2013年10月, [査読有り]
英語, 研究論文(学術雑誌), Response of phytoplankton to increasing CO2 in seawater in terms of physiology and ecology is key to predicting changes in marine ecosystems. However, responses of natural plankton communities especially in the open ocean to higher CO2 levels have not been fully examined. We conducted CO2 manipulation experiments in the Bering Sea and the central subarctic Pacific, known as high nutrient and low chlorophyll regions, in summer 2007 to investigate the response of organic matter production in iron-deficient plankton communities to CO2 increases. During the 14-day incubations of surface waters with natural plankton assemblages in microcosms under multiple pCO(2) levels, the dynamics of particulate organic carbon (POC) and nitrogen (PN), and dissolved organic carbon (DOC) and phosphorus (DOP) were examined with the plankton community compositions. In the Bering site, net production of POC, PN, and DOP relative to net chlorophyll-a production decreased with increasing pCO(2). While net produced POC:PN did not show any CO2-related variations, net produced DOC:DOP increased with increasing pCO(2). On the other hand, no apparent trends for these parameters were observed in the Pacific site. The contrasting results observed were probably due to the different plankton community compositions between the two sites, with plankton biomass dominated by large-sized diatoms in the Bering Sea versus ultra-eukaryotes in the Pacific Ocean. We conclude that the quantity and quality of the production of particulate and dissolved organic matter may be altered under future elevated CO2 environments in some iron-deficient ecosystems, while the impacts may be negligible in some systems. - Effects of pCO2 and iron on the elemental composition and cell geometry of the marine diatom Pseudo-nitzschia pseudodelicatissima (Bacillariophyceae)
Koji Sugie, Takeshi Yoshimura
JOURNAL OF PHYCOLOGY, 49, 3, 475, 488, WILEY-BLACKWELL, 2013年06月, [査読有り]
英語, 研究論文(学術雑誌), Partial pressure of CO2 (pCO2) and iron availability in seawater show corresponding changes due to biological and anthropogenic activities. The simultaneous change in these factors precludes an understanding of their independent effects on the ecophysiology of phytoplankton. In addition, there is a lack of data regarding the interactive effects of these factors on phytoplankton cellular stoichiometry, which is a key driving factor for the biogeochemical cycling of oceanic nutrients. Here, we investigated the effects of pCO2 and iron availability on the elemental composition (C, N, P, and Si) of the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle by dilute batch cultures under 4 pCO2 (similar to 200, similar to 380, similar to 600, and similar to 800atm) and five dissolved inorganic iron (Fe; similar to 5, similar to 10, similar to 20, similar to 50, and similar to 100pmol center dot L-1) conditions. Our experimental procedure successfully overcame the problems associated with simultaneous changes in pCO2 and Fe by independently manipulating carbonate chemistry and iron speciation, which allowed us to evaluate the individual effects of pCO2 and iron availability. We found that the C:N ratio decreased significantly only with an increase in Fe, whereas the C:P ratio increased significantly only with an increase in pCO2. Both Si:C and Si:N ratios decreased with increasing pCO2 and Fe. Our results indicate that changes in pCO2 and iron availability could influence the biogeochemical cycling of nutrients in future oceans with high- CO2 levels, and, similarly, during the time course of phytoplankton blooms. Moreover, pCO2 and iron availability may also have affected oceanic nutrient biogeochemistry in the past, as these conditions have changed markedly over the Earth's history. - Culture of the hydrocarbon producing microalga Botryococcus braunii strain Showa: Optimal CO2, salinity, temperature, and irradiance conditions
Takeshi Yoshimura, Shigeru Okada, Masaki Honda
BIORESOURCE TECHNOLOGY, 133, 232, 239, ELSEVIER SCI LTD, 2013年04月, [査読有り]
英語, 研究論文(学術雑誌), Specific growth rates and hydrocarbon contents of Botryococcus braunii strain Showa were measured under a wide range of CO2, salinity, temperature, and irradiance conditions. The bubbling CO2 concentration of 0.2-5% and no addition of salinity were favorable conditions for growth. The strain cannot grow at 5 degrees C and above 35 degrees C under any irradiance levels. Maximum specific growth rate of 0.5 day(-1) (doubling time of 1.4 days), the highest value reported for B. braunii in the past studies, was observed at 30 degrees C and 850 mu mol photons m(-2) s(-1). Since hydrocarbon productivity, shown as the product of hydrocarbon content and specific growth rate, increased with the increasing specific growth rate, we conclude that more efficient hydrocarbon production by the mass culture of strain Showa can be achieved by maintaining higher specific growth rate based on the culture conditions presented in this study. (C) 2013 Elsevier Ltd. All rights reserved. - Appropriate bottles for storing seawater samples for dissolved organic phosphorus (DOP) analysis: A step toward the development of DOP reference materials
Takeshi Yoshimura
LIMNOLOGY AND OCEANOGRAPHY-METHODS, 11, APR, 239, 246, AMER SOC LIMNOLOGY OCEANOGRAPHY, 2013年04月, [査読有り]
英語, 研究論文(学術雑誌), Reference materials (RMs) are essential to ensure comparability of oceanographic chemical measurements. Since dissolved organic matter (DOM) plays a significant role in bioactive element cycles such as carbon (C), nitrogen (N), and phosphorus (P), measurements of dissolved organic C, N, and P (DOC, DON, and DOP) have been paid much attention. Although RMs that target DOC and DON analyses have been developed, no comparable studies have been conducted for DOP analysis. This study examined the selection of the most appropriate bottle to store DOP samples to develop an RM for DOP. Natural seawater samples were stored in bottles made from various plastic materials and glass, and the temporal change in DOP and DOC concentrations were measured during a storage period of months to a year in the dark under 25 degrees C. I found that severe contaminations of DOP and/or DOC occurred in some bottle types. Whereas DOP concentrations were unchanged in many bottle types, the concentrations of both DOP and DOC were stable only in glass and perfluoroalkoxy (PFA) bottles. I conclude that these two bottle types have potential as an appropriate sample bottle for RMs of DOP, this is because colloids formed via DOC contamination during storage can potentially bond with phosphate in the sample and thus inorganic phosphate may be measured as DOP contamination. - Synergistic effects of pCO2 and iron availability on nutrient consumption ratio of the Bering Sea phytoplankton community
K. Sugie, H. Endo, K. Suzuki, J. Nishioka, H. Kiyosawa, T. Yoshimura
Biogeosciences Discussions, 10, 3, 4331, Copernicus {GmbH}, 2013年03月 - Wet extraction of hydrocarbons from Botryococcus braunii by dimethyl ether as compared with dry extraction by hexane
Hideki Kanda, Peng Li, Takeshi Yoshimura, Shigeru Okada
FUEL, 105, 535, 539, ELSEVIER SCI LTD, 2013年03月, [査読有り]
英語, 研究論文(学術雑誌), We propose the use of liquid dimethyl ether (DME) as a solvent for extracting hydrocarbons and lipids from Botryococcus braunii Race B paste. The extraction yields and main elements of the extracts obtained from the B. braunii by our technique is nearly the same as by hexane Soxhlet extraction on dried mass. A qualitative analysis by silica gel thin layer chromatography suggested that DME also effectively extracted the specific hydrocarbons, botryococcenes from the wet B. braunii cells. Gas chromatography/mass spectrometry of DME extracts indicated the presence of a large amount of C32-C34 botryococcenes-the main components of B. braunii Race B. In contrast, hexane extracted not only C32-C34, but also small amounts of C30 and C31 botryococcenes-the minor components. The result shows that extraction differences between DME and hexane are not important. (C) 2012 Elsevier Ltd. All rights reserved. - Synergistic effects of p CO2 and iron availability on nutrient consumption ratio of the Bering Sea phytoplankton community
K. Sugie, H. Endo, K. Suzuki, J. Nishioka, H. Kiyosawa, T. Yoshimura
Biogeosciences, 10, 10, 6309, 6321, Copernicus {GmbH}, 2013年, [査読有り]
英語, 研究論文(学術雑誌), Little is known concerning the effect of CO2 on phytoplankton ecophysiological processes under nutrient and trace element-limited conditions, because most CO2 manipulation experiments have been conducted under elements-replete conditions. To investigate the effects of CO2 and iron availability on phytoplankton ecophysiology, we conducted an experiment in September 2009 using a phytoplankton community in the iron limited, high-nutrient, low-chlorophyll (HNLC) region of the Bering Sea basin . Carbonate chemistry was controlled by the bubbling of the several levels of CO2 concentration (180, 380, 600, and 1000 ppm) controlled air, and two iron conditions were established, one with and one without the addition of inorganic iron. We demonstrated that in the iron-limited control conditions, the specific growth rate and the maximum photochemical quantum efficiency (<
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m) of photosystem (PS) II decreased with increasing CO2 levels, suggesting a further decrease in iron bioavailability under the high-CO2 conditions. In addition, biogenic silica to particulate nitrogen and biogenic silica to particulate organic carbon ratios increased from 2.65 to 3.75 and 0.39 to 0.50, respectively, with an increase in the CO2 level in the iron-limited controls. By contrast, the specific growth rate, <
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m values and elemental compositions in the iron-added treatments did not change in response to the CO2 variations, indicating that the addition of iron canceled out the effect of the modulation of iron bioavailability due to the change in carbonate chemistry. Our results suggest that high-CO2 conditions can alter the biogeochemical cycling of nutrients through decreasing iron bioavailability in the iron-limited HNLC regions in the future. © Author(s) 2013. - Synergistic effects of pCO(2) and iron availability on nutrient consumption ratio of the Bering Sea phytoplankton community
K. Sugie, H. Endo, K. Suzuki, J. Nishioka, H. Kiyosawa, T. Yoshimura
BIOGEOSCIENCES, 10, 10, 6309, 6321, COPERNICUS GESELLSCHAFT MBH, 2013年, [査読有り]
英語, 研究論文(学術雑誌), Little is known concerning the effect of CO2 on phytoplankton ecophysiological processes under nutrient and trace element-limited conditions, because most CO2 manipulation experiments have been conducted under elements-replete conditions. To investigate the effects of CO2 and iron availability on phytoplankton ecophysiology, we conducted an experiment in September 2009 using a phytoplankton community in the iron limited, high-nutrient, low-chlorophyll (HNLC) region of the Bering Sea basin. Carbonate chemistry was controlled by the bubbling of the several levels of CO2 concentration (180, 380, 600, and 1000 ppm) controlled air, and two iron conditions were established, one with and one without the addition of inorganic iron. We demonstrated that in the iron-limited control conditions, the specific growth rate and the maximum photochemical quantum efficiency (F-v/F-m) of photosystem (PS) II decreased with increasing CO2 levels, suggesting a further decrease in iron bioavailability under the high-CO2 conditions. In addition, biogenic silica to particulate nitrogen and biogenic silica to particulate organic carbon ratios increased from 2.65 to 3.75 and 0.39 to 0.50, respectively, with an increase in the CO2 level in the iron-limited controls. By contrast, the specific growth rate, F-v/F-m values and elemental compositions in the iron-added treatments did not change in response to the CO2 variations, indicating that the addition of iron canceled out the effect of the modulation of iron bioavailability due to the change in carbonate chemistry. Our results suggest that high-CO2 conditions can alter the biogeochemical cycling of nutrients through decreasing iron bioavailability in the iron-limited HNLC regions in the future. - Effects of CO2 and iron availability on phytoplankton and eubacterial community compositions in the northwest subarctic Pacific
H. Endo, T. Yoshimura, T. Kataoka, K. Suzuki
Journal of Experimental Marine Biology and Ecology, 439, 160, 175, 2013年01月, [査読有り]
英語, 研究論文(学術雑誌), On-deck CO2-Fe-manipulated incubation experiments were conducted using surface seawater collected from the Western Subarctic Gyre of the NW Pacific in the summer of 2008 to elucidate the impacts of ocean acidification and Fe enrichment on the abundance and community composition of phytoplankton and eubacteria in the study area. During the incubation, excluding the initial period, the mean partial pressures of CO2 in non-Fe-added bottles were 230, 419, 843, and 1124μatm, whereas those in Fe-added treatments were 152, 394, 791, and 1008μatm. Changes in the abundance and community composition of phytoplankton were estimated using HPLC pigment signatures with the program CHEMTAX and flow cytometry. A DGGE fingerprint technique targeting 16S rRNA gene fragments was also used to estimate changes in eubacterial phylotypes during incubation. The Fe addition induced diatom blooms, and subsequently stimulated the growth of heterotrophic bacteria such as Roseobacter, Phaeobacter, and Alteromonas in the post-bloom phase. In both the Fe-limited and Fe-replete treatments, concentrations of 19'-hexanoyloxyfucoxanthin, a haptophyte marker, and the cell abundance of coccolithophores decreased at higher CO2 levels (750 and 1000ppm), whereas diatoms exhibited little response to the changes in CO2 availability. The abundances of Synechococcus and small eukaryotic phytoplankton (<
10μm) increased at the higher CO2 levels. DGGE band positions revealed that Methylobacterium of Alphaproteobacteria occurred solely at lower CO2 levels (180 and 380ppm) during the post-bloom phase. These results suggest that increases in CO2 level could affect not only the community composition of phytoplankton but also that of eubacteria. As these microorganisms play critical roles in the biological carbon pump and microbial loop, our results indicate that the progression of ocean acidification can alter the biogeochemical processes in the study area. © 2012 Elsevier B.V. - 海洋酸性化が植物プランクトンの動態および物質循環に及ぼす影響
杉江 恒二, 芳村 毅, Koji Sugie, Takeshi Yoshimura, 財, 電力中央研究所環境科学研究所, 財)電力中央研究所環境科学研究所, Central Research Institute of Electric Power Industry, Central Research Institute of Electric Power Industry
海の研究 = Umi no Kenkyu (Oceanography in Japan), 20, 5, 101, 148, 日本海洋学会, 2011年09月15日
日本語, 海洋酸性化が海洋生物に及ぼす影響に関する研究が近年勢力的に行われている。本総説では,先ず,地球史における海水のpHの変遷と現代の海洋酸性化とを対比しながら植物プランクトンの動態について考察した。続いて,海洋酸性化の実験方法および植物プランクトンの生理生態と物質循環に及ぼす影響に関して近年の報告を中心にまとめ,以下の課題を抽出した。(1)過去の海洋酸性化の研究において亜寒帯や寒帯および外洋性の単離培養株が用いられていないこと,生息域や生活環に基づく実験が行われていないことは,自然環境における海洋酸性化の影響を把握する上での知識の欠如となっている。(2)pHの変化によって鉄と錯形成をする有機配位子の化学形態や2価鉄の濃度が変化するため,それらが生態系に及ぼす影響を評価する必要がある。(3)pHの低下により植物プランクトン細胞の有機炭素:リン比は増加する傾向,有機炭素:窒素比はほとんど変化しない傾向にある。一方では,pHの低下が溶存有機物およびケイ素の動態に与える影響には未解明な点が多いため,研究を促進させる必要がある。(4)pHの低下と鉄などの微量元素の利用性との複合作用がシアノバクテリアの窒素固定速度に及ぼす影響を明らかにし,窒素循環過程の理解を深化させる必要がある。Increasing atmospheric CO_2 over the Anthropocene due to human activities has reduced seawater pH and it continues as long as burning fossil fuels. In this review, we summarize and discuss the methodology of ocean acidification study and the effects of ocean acidification on phytoplankton and biogeochemical cycle of nutrients. In addition, we compare the pH variability and phytoplankton dynamics over the past 60 million years with the recent phenomenon of ocean acidification. We found that: (1) there is a significant lack of knowledge on the effect of ocean acidification because previous studies were not examined using subpolar to polar phytoplankton species and also not considered their habitat and life cycle. (2) We should study the effect of pH on dissolved inorganic Fe(II) bioavailability relative to dissolved inorganic Fe(III) species and the availability of Fe(III)-ligand complex. The role of Fe(II) and chemical structure of the ligand are also important issues. (3) Particulate organic carbon to phosphorus ratio increased in response to the decrease in pH whereas particulate organic carbon to nitrogen ratio was unaffected by pH. However, there is a lack of knowledge with regard to the effect of ocean acidification on dissolved organic matter and silicic acid dynamics. (4) We should study further the interactive effects of pH and trace metals especially for iron on N_2 fixation rate by cyanobacteria to improve our knowledge about nitrogen cycle. - Seasonal phosphorus depletion and microbial responses to the change in phosphorus availability in a subarctic coastal environment
Takeshi Yoshimura, Isao Kudo
MARINE CHEMISTRY, 126, 1-4, 182, 192, ELSEVIER SCIENCE BV, 2011年09月, [査読有り]
英語, 研究論文(学術雑誌), Temporal variations of phosphorus (P) pools (soluble reactive P (SRP), dissolved organic P (DOP), and particulate P (PP)), alkaline phosphatase activity (APA), and orthophosphate uptake were measured in Funka Bay to describe the annual P cycle in the bay and determine the phytoplankton and bacterial responses to the change in P availability. Observations over 4 years demonstrated that SRP concentrations in the surface waters decreased from the winter maximum of 1.0 mu mol L(-1) to 0.3 mu mol L(-1) at the end of the spring phytoplankton bloom, and then to under the detection limit by around August. The DOP concentration was relatively constant at 0.1 mu mol L(-1) throughout the year with higher concentrations up to 0.3 mu mol L(-1) just after the peak of the spring bloom. PP concentrations varied around 0.1 mu mol L(-1) with higher concentrations up to 0.6 mu mol L(-1) during the high chlorophyll a concentration period. SRP comprised 85% of the total P pool prior to the spring bloom, but the contribution decreased to less than 30% in the post-bloom period and DOP dominated the P pool in the surface. Most of the measured APA was less than 0.1 nmol L(-1) min(-1) in the whole water column, but exceptionally high values up to 1.4 nmol L(-1) min(-1) were observed in low SRP surface waters during summer. When SRP decreased from 0.3 mu mol L(-1) in spring to under the detection limit in summer, turnover time for orthophosphate at 10 m depth decreased from 61 days to 4.2 h. The high APA and short turnover time for orthophosphate suggested that microbes in the surface mixed layer experienced severe P stress in summer. Under severely P stressed conditions, APA was detected mostly in the >10 mu m fraction, suggesting that large phytoplankton would acquire P from DOP to overcome the P stress. This was supported by the result that large phytoplankton were outcompeted by the 0.2-1 mu m sized microbes for orthophosphate uptake. (C) 2011 Elsevier B.V. All rights reserved. - Oceanic iron supply mechanisms which support the spring diatom bloom in the Oyashio region, western subarctic Pacific
Jun Nishioka, Tsuneo Ono, Hiroaki Saito, Keiichiro Sakaoka, Takeshi Yoshimura
JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS, 116, 2, AMER GEOPHYSICAL UNION, 2011年02月, [査読有り]
英語, 研究論文(学術雑誌), Multiyear (2003-2008) time series observations along the A line provided information on the temporal variability of the dissolved iron (diss-Fe) concentration in the Oyashio region of the western subarctic Pacific, and the data indicated that there was an annual cycle in the concentration of surface diss-Fe occurring every year. Diss-Fe was supplied into the surface water in this region every winter and supports the spring phytoplankton bloom after development of the thermocline. The diss-Fe concentration was drawn down during the phytoplankton bloom period and was depleted in summer in some water masses. Then diss-Fe increased from autumn to winter with the increasing depth of the surface mixed layer. The high diss-Fe concentrations in the surface layer in winter were controlled by mesoscale oceanic intrinsic processes, such as vertical winter mixing and horizontal Fe-rich intermediate water transport. Difference in magnitude of the winter mixing processes among different water masses caused the heterogeneous distribution of diss-Fe concentration in the surface layer. Moreover, the vertical section profiles along a cross-Oyashio transect showed the occurrence of Fe-rich intermediate water, and upward transport of materials from the intermediate water to the surface layer via tidal and winter mixing processes are important mechanisms to explain the high winter surface diss-Fe concentrations. Additionally, the substantially higher diss-Fe/NO3 ratio in the winter surface layer in this studied area other than the high-nutrient low-chlorophyll region indicates that the winter surface water in the Oyashio and the Oyashio-Kuroshio transition zone has a high potential to stimulate phytoplankton growth. - Oceanic iron supply mechanisms which support the spring diatom bloom in the Oyashio region, western subarctic Pacific
Jun Nishioka, Tsuneo Ono, Hiroaki Saito, Keiichiro Sakaoka, Takeshi Yoshimura
Journal of Geophysical Research: Oceans, 116, 2, Blackwell Publishing Ltd, 2011年, [査読有り]
英語, 研究論文(学術雑誌), Multiyear (2003-2008) time series observations along the A line provided information on the temporal variability of the dissolved iron (diss-Fe) concentration in the Oyashio region of the western subarctic Pacific, and the data indicated that there was an annual cycle in the concentration of surface diss-Fe occurring every year. Diss-Fe was supplied into the surface water in this region every winter and supports the spring phytoplankton bloom after development of the thermocline. The diss-Fe concentration was drawn down during the phytoplankton bloom period and was depleted in summer in some water masses. Then diss-Fe increased from autumn to winter with the increasing depth of the surface mixed layer. The high diss-Fe concentrations in the surface layer in winter were controlled by mesoscale oceanic intrinsic processes, such as vertical winter mixing and horizontal Fe-rich intermediate water transport. Difference in magnitude of the winter mixing processes among different water masses caused the heterogeneous distribution of diss-Fe concentration in the surface layer. Moreover, the vertical section profiles along a cross-Oyashio transect showed the occurrence of Fe-rich intermediate water, and upward transport of materials from the intermediate water to the surface layer via tidal and winter mixing processes are important mechanisms to explain the high winter surface diss-Fe concentrations. Additionally, the substantially higher diss-Fe/NO3 ratio in the winter surface layer in this studied area other than the high-nutrient low-chlorophyll region indicates that the winter surface water in the Oyashio and the Oyashio-Kuroshio transition zone has a high potential to stimulate phytoplankton growth. Copyright © 2011 by the American Geophysical Union. - Oceanic iron supply mechanisms which supprt the spring diatom bloom in the Oyashio region, western subarctic pacific
Nishioka J, Ono T, Saito H, Sakaoka K, Yoshimura T
Journal of Geophysical Research, 116, C2, C02021, 2011年, [査読有り]
英語, 研究論文(学術雑誌) - 夏季ベーリング海における植物プランクトン群集へのCO2と鉄の効果がSiとNの利用比に及ぼす影響.
杉江恒二, 遠藤寿, 鈴木光次, 芳村毅
月間海洋, 43, 12, 735, 741, 2011年
日本語 - Iron nutritional status of the phytoplankton assemblage in the Okhotsk Sea during summer
Takeshi Yoshimura, Jun Nishioka, Takeshi Nakatsuka
DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS, 57, 11, 1454, 1464, PERGAMON-ELSEVIER SCIENCE LTD, 2010年11月, [査読有り]
英語, 研究論文(学術雑誌), To clarify the iron (Fe) nutritional status of the phytoplankton assemblage in the Okhotsk Sea we conducted incubation experiments in summer 2006 Replicate surface seawater samples with the natural plankton community were incubated with three treatments Fe enrichment addition of the strong Fe chelator siderophore desferriferrioxime B (DFB) which strips Fe from the biologically accessible pool and as a control no addition To prevent macronutrient limitation we added surplus nutrients to all treatments At all 4 stations in Sakhalin Bay near the mouth of the Amur River and around the east of Sakhalin Island net specific growth rate showed no significant difference between the control and +Fe treatment and was repressed in +DFB treatment both in large- and small-sized phytoplankton These findings indicate that these waters contain sufficient bioavailable Fe and that the Amur River plume which is transported by the east Sakhalin current is a major source of the Fe In the Bussol Strait net specific growth rate in the control was significantly higher than +DFB treatment suggesting a supply of bioavailable Fe through intense vertical mixing at this site Iron enrichment treatment stimulated the net specific growth rate of large-sized phytoplankton indicating that Fe still limits the growth for the large-sized phytoplankton assemblage but not for small-sized phytoplankton in this area An Index of Fe availability was defined to quantify the degree of ambient Fe availability in each station and it revealed the spatial variability of ambient Fe availabilities among the sites (C) 2010 Elsevier Ltd All rights reserved - Impacts of elevated CO2 on organic carbon dynamics in nutrient depleted Okhotsk Sea surface waters
Takeshi Yoshimura, Jun Nishioka, Koji Suzuki, Hiroshi Hattori, Hiroshi Kiyosawa, Yutaka W. Watanabe
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 395, 1-2, 191, 198, ELSEVIER SCIENCE BV, 2010年11月, [査読有り]
英語, 研究論文(学術雑誌), Increasing CO2 in seawater (i.e. ocean acidification) may have various and potentially adverse effects on phytoplankton dynamics and hence the organic carbon dynamics. We conducted a CO2 manipulation experiment in the Sea of Okhotsk in summer 2006 to investigate the response of the organic carbon dynamics. During the 14-day incubation of nutrient depleted and 200 mu atm in situ pCO(2) surface water with a natural plankton assemblage under 150, 280, 480, and 590 mu atm pCO(2), the amount of net dissolved organic carbon accumulation was significantly lower at >480 mu atm pCO(2) than at 150 mu atm pCO(2), while differences in net particulate organic carbon accumulation between the treatments were small and did not show a clear relationship with the pCO(2). This is the first report to show a decreased net organic carbon production of natural plankton community under elevated pCO(2). Phytoplankton pigment analysis suggests that the relative contribution of fucoxanthin-containing phytoplankton such as diatoms to the phytoplankton biomass was lower at >280 mu atm pCO(2) than at 150 mu atm pCO(2). Different pCO(2) conditions may alter the organic carbon dynamics through changes in plankton processes. We conclude that the continuing increase in atmospheric CO2 in a time scale from the last half century to the end of this century has potential to affect the carbon cycle in nutrient depleted subpolar surface waters. (C) 2010 Elsevier B.V. All rights reserved. - The annual cycle of surface iron and the source of iron supporting the spring diatom bloom in the Oyashio region, western subarctic Pacific
Nishioka J, Ono T, Saito H, Sakaoka K, Yoshimura T, Matoba
AGU Fall Meeting Abstracts, 1, 0331, 2010年, [査読有り] - Dynamics and elemental stoichiometry of carbon, nitrogen, and phosphorus in particulate and dissolved organic pools during a phytoplankton bloom induced by in situ iron enrichment in the western subarctic Pacific (SEEDS-II)
Takeshi Yoshimura, Hiroshi Ogawa, Keiri Imai, Takafumi Aramaki, Yukihiro Nojiri, Jun Nishioka, Atsushi Tsuda
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 56, 26, 2863, 2874, PERGAMON-ELSEVIER SCIENCE LTD, 2009年12月, [査読有り]
英語, 研究論文(学術雑誌), The dynamics of organic carbon (C), nitrogen (N), and phosphorus (P) were examined during an in situ mesoscale iron-enrichment experiment in the western North Pacific in the summer of 2004. We separately determined the production of particulate organic matter (POM) and dissolved organic matter (DOM) and their subsequent removal during the bloom decline. As the iron-induced phytoplankton bloom progressed (days 0-14), POM increased in the surface mixed layer, while DOM did not increase significantly. The molar ratios for C:N, C:P, and N:P of the newly produced POM were estimated to be 4.9,190, and 37 in the surface mixed layer, whereas the dissolved inorganic nitrogen to soluble reactive phosphorus drawdown ratio was 17. Preferential remineralization of P over C and N from the POM was postulated during the developing phytoplankton bloom. During the bloom decline (days 16-25), surface POM decreased with a similar C:N of 5.2. The N:P ratio of surface DOM increased during the bloom decline. Below the surface mixed layer, DOC and DON increased moderately after the peak of the bloom. The time-series variation of DOC and DON was not identical. The C, N, and P dynamics through the accumulation and removal of POM and DOM were complex. Grazing by mesozooplankton during the experiment may have played a significant role in the uncoupling of the dynamics of C, N, and P. (C) 2009 Elsevier Ltd. All rights reserved. - Biogeochemical cycling of N and Si during the mesoscale iron-enrichment experiment in the western subarctic Pacific (SEEDS-II)
Hiroaki Saito, Atsushi Tsuda, Yukihiro Nojiri, Takafumi Aramaki, Hiroshi Ogawa, Takeshi Yoshimura, Keiri Imai, Isao Kudo, Jun Nishioka, Tsuneo Ono, Koji Suzuki, Shigenobu Takeda
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 56, 26, 2852, 2862, PERGAMON-ELSEVIER SCIENCE LTD, 2009年12月, [査読有り]
英語, 研究論文(学術雑誌), Biogeochemical cycles of N and Si were examined in the surface mixed layer during the mesoscale iron-enrichment (IE) experiment in the high-nutrient low-chlorophyll (HNLC) western subarctic Pacific (SEEDS-II). Although the IEs increased nitrate uptake, silicic acid utilization was not stimulated. The nitrate drawdown in the iron-patch (IN-patch, 140.3 mmol m(-2) in the surface mixed layer, 0-30 in) was only 25% of the initial inventory, which was 1/3-2/5 of the previous IE experiments in the subarctic Pacific. This relatively weak response of nutrient drawdown to IEs was due to the high biomass of mesozooplankton (MZ) dominated by copepod Neocalanus; plumchrus. Feeding of MZ (247.2 mmol m(-2) during Day 0-21 from the first IE) in the IN-patch was higher than the nitrate drawdown and prevented further development of the phytoplankton bloom. In the later period of the experiment (Day 14-21) the increase in the feeding activity and resultant decrease in phytoplankton biomass induced the accumulation of dissolved organic nitrogen (DON) and ammonium. Among total growth of MZ (81.6 mmol N m(-2)), 89% (72.8 mmol N m(-2)) was transported to the depth by the ontogenetic downward migration of N. plumchrus. Although silicic acid drawdown was not increased by the IEs, Si export flux increased by 2.7 times. The increase in Si export was also due to the increase in MZ, which egested faecal pellets with higher Si:N ratio and faster sinking speed than diatoms. The export efficiency (78% of new production) and total amount of export flux (143.8 mmol N m(-2) 1392 mmol C m(-2)) were highest records within the IE experiments despite weak responses of nutrient drawdown to the IE. During SEEDS-II, the high biomass of MZ reduced the phytoplankton response and nutrient drawdown to the IEs but via grazing and ontogenetic vertical migration accelerated the export flux as well as accumulations of dissolved forms of N. Results of the present and previous IE experiments indicate that the ecosystem and biogeochemical responses to IEs in the HNLC region are quite sensitive to the ecosystem components, especially for grazers of diatoms such as copepods and heterotrophic dinoflagellates. More attention needs to be paid to the ecosystem components and their biogeochemical functions as well as physical and chemical properties of the ecosystems in order to hindcast or forecast the impacts of changes in atmospheric iron deposition. (C) 2009 Elsevier Ltd. All rights reserved. - Impacts of elevated CO2 on phytoplankton community composition and organic carbon dynamics in nutrient-depleted Okhotsk Sea surface waters
Yoshimura T., Nishioka J., Suzuki K., Hattori H., Kiyosawa H., Watanabe Y. W.
Biogeosciences Discussions, 6, 2, 4143, 4163, Copernicus Publications, 2009年04月15日
英語, Impacts of the increasing CO2 in seawater (i.e. ocean acidification) on phytoplankton physiology may have various and potentially adverse effects on phytoplankton dynamics and the carbon cycle. We conducted a CO2 manipulation experiment in the Sea of Okhotsk in summer 2006 to investigate the response of the phytoplankton assemblage and dynamics of organic carbon. During the 14-day incubation of nutrient-depleted surface water with a natural phytoplankton assemblage under 150, 280, 480, and 590μatm pCO2, the relative abundance of fucoxanthin-containing phytoplankton such as diatoms and prymnesiophytes decreased with increasing pCO2. The amount of DOC accumulation also decreased with increasing pCO2, while differences in POC accumulation between the treatments were small and did not show a clear trend with the pCO2. Change in the phytoplankton community composition under different pCO2 conditions will alter the organic carbon dynamics as found in the present experiment. Compared to results in the literature from nutrient-replete conditions indicating a potential enhancement of phytoplankton production with elevated pCO2, the present results indicated a different physiological response of phytoplankton under nutrient-depleted conditions. These results indicate that the continuing increase in atmospheric CO2 can significantly affect the structure of marine ecosystems and carbon cycle in nutrient-depleted subpolar surface waters. - Evidence for the grazing hypothesis: Grazing reduces phytoplankton responses of the HNLC ecosystem to iron enrichment in the western subarctic pacific (SEEDS II)
Atsushi Tsuda, Shigenobu Takeda, Hiroaki Saito, Jun Nishioka, Isao Kudo, Yukihiro Nojiri, Koji Suzuki, Mitsuo Uematsu, Mark L. Wells, Daisuke Tsumune, Takeshi Yoshimura, Tatsuo Aono, Takafumi Aramaki, William P. Cochlan, Maki Hayakawa, Keiri Imai, Tomoshi Isada, Yoko Iwamoto, William K. Johnson, Sohiko Kameyama, Shungo Kato, Hiroshi Kiyosawa, Yoshiko Kondo, Maurice Levasseur, Ryuji J. Machida, Ippei Nagao, Fumiko Nakagawa, Takahiro Nakanish, Seiji Nakatsuka, Akira Narita, Yoshifumi Noiri, Hajime Obata, Hiroshi Ogawa, Kenji Oguma, Tsuneo Ono, Tomofumi Sakuragi, Motoki Sasakawa, Mitsuhide Sato, Akifumi Shimamoto, Hyoe Takata, Charles G. Trick, Yutaka W. Watanabe, Chi Shing Wong, Naoki Yoshie
JOURNAL OF OCEANOGRAPHY, 63, 6, 983, 994, SPRINGER, 2007年12月, [査読有り]
英語, 研究論文(学術雑誌), mesoscale iron-enrichment study (SEEDS II) was carried out in the western subarctic Pacific in the summer of 2004. The iron patch was traced for 26 days, which included observations of the development and the decline of the bloom by mapping with sulfur hexafluoride. The experiment was conducted at almost the same location and the same season as SEEDS (previous iron-enrichment experiment). However, the results were very different between SEEDS and SEEDS II. A high accumulation of phytoplankton biomass (similar to 18 mg chl m(-3)) was characteristic of SEEDS. In contrast, in SEEDS II, the surface chlorophyll-a accumulation was lower, 0.8 to 2.48 mg m(-3), with no prominent diatom bloom. Photosynthetic competence in terms of F-v/F-m for the total phytoplankton community in the surface waters increased after the iron enrichments and returned to the ambient level by day 20. These results suggest that the photosynthetic physiology of the phytoplankton assemblage was improved by the iron enrichments and returned to an iron-stressed condition during the declining phase of the bloom. Pico-phytoplankton (< 2 mu m) became dominant in the chlorophyll-a size distribution after the bloom. We observed a nitrate drawdown of 3.8 mu M in the patch (day 21), but there was no difference in silicic acid concentration between inside and outside the patch. Mesozooplankton (copepod) biomass was three to five times higher during the bloom-development phase in SEEDS II than in SEEDS. The copepod biomass increased exponentially. The grazing rate estimation indicates that the copepod grazing prevented the formation of an extensive diatom bloom, which was observed in SEEDS, and led to the change to a pico-phytoplankton dominated community towards the end of the experiment. - Iron supply to the western subarctic Pacific: Importance of iron export from the Sea of Okhotsk
Jun Nishioka, Tsuneo Ono, Hiroaki Saito, Takeshi Nakatsuka, Shigenobu Takeda, Takeshi Yoshimura, Koji Suzuki, Kenshi Kuma, Shigeto Nakabayashi, Daisuke Tsumune, Humio Mitsudera, W. Keith Johnson, Atsushi Tsuda
Journal of Geophysical Research: Oceans, 112, 10, Blackwell Publishing Ltd, 2007年10月08日, [査読有り]
英語, 研究論文(学術雑誌), Iron is an essential nutrient and plays an important role in the control of phytoplankton growth (Martin et al., 1989). Atmospheric dust has been thought to be the most important source of iron, supporting annual biological production in the western subarctic Pacific (WSP) (Duce and Tindale, 1991
Moore et al., 2002). We argue here for another source of iron to the WSP. We found extremely high concentrations of dissolved and particulate iron in the Okhotsk Sea Intermediate Water (OSIW) and the North Pacific Intermediate Water (NPIW), and water ventilation processes in this region probably control the transport of iron through the intermediate water layer from the continental shelf of the Sea of Okhotsk to wide areas of the WSP. Additionally, our time series data in the Oyashio region of the WSP indicate that the pattern of seasonal changes in dissolved iron concentrations in the surface-mixed layer was similar to that of macronutrients, and that deep vertical water mixing resulted in higher winter concentrations of iron in the surface water of this region. The estimated dissolved iron supply from the iron-rich intermediate waters to the surface waters in the Oyashio region was comparable to or higher than the reported atmospheric dust iron input and thus a major source of iron to these regions. Our data suggest that the consideration of this source of iron is essential in our understanding of spring biological production and biogeochemical cycles in the western subarctic Pacific and the role of the marginal sea. Copyright 2007 by the American Geophysical Union. - Nutrient regeneration at bottom after a massive spring bloom in a subarctic coastal environment, Funka Bay, Japan
Isao Kudo, Takeshi Yoshimura, Choon-Weng Lee, Mitsuru Yanada, Yoshiaki Maita
JOURNAL OF OCEANOGRAPHY, 63, 5, 791, 801, SPRINGER, 2007年10月, [査読有り]
英語, 研究論文(学術雑誌), Nutrient regeneration and oxygen consumption after a spring bloom in Funka Bay were studied on monthly survey cruises from February to November 1998 and from March to December 1999. A high concentration of ammonium (more than 4 mu mol 1-1) was observed near the bottom (80-90 m) after April. Phosphate and silicate gradually accumulated and dissolved oxygen decreased in the same layer. Salinity near the bottom did not change until summer, leading to the presumption that the system in this layer is semi-closed, so regenerated nutrients were preserved until September. Nitrification due to the oxidation of ammonium to nitrate was observed after June. Nitrite, an intermediate product, was detected at 4-7 mu mol L(-1) in June and July 1999. Assuming that decomposition is a first order reaction, the rate constant for decomposition of organic nitrogen was determined to be 0.014 and 0.008 P in 1998 and 1999, respectively. The ammonium oxidation rate increased rapidly when the ambient ammonium concentration exceeded 5,mu mol L-1. We also performed a budget calculation for the regeneration process. The total amount of N regenerated in the whole water column was 287.4 mmol N m(-2) in 4 months, which is equal to 22.8 gC m(-2), assuming the Redfield C to N ratio. This is 34% of the primary production during the spring bloom and is comparable to the export production of 25 gC m-2 measured by a sediment trap at 60 m. - Iron supply to the western subarctic Pacific: Importance of iron export from the Sea of Okhotsk
Jun Nishioka, Tsuneo Ono, Hiroaki Saito, Takeshi Nakatsuka, Shigenobu Takeda, Takeshi Yoshimura, Koji Suzuki, Kenshi Kuma, Shigeto Nakabayashi, Daisuke Tsumune, Humio Mitsudera, W. Keith Johnson, Atsushi Tsuda
JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS, 112, C10, AMER GEOPHYSICAL UNION, 2007年10月, [査読有り]
英語, 研究論文(学術雑誌), Iron is an essential nutrient and plays an important role in the control of phytoplankton growth ( Martin et al., 1989). Atmospheric dust has been thought to be the most important source of iron, supporting annual biological production in the western subarctic Pacific (WSP) (Duce and Tindale, 1991; Moore et al., 2002). We argue here for another source of iron to the WSP. We found extremely high concentrations of dissolved and particulate iron in the Okhotsk Sea Intermediate Water (OSIW) and the North Pacific Intermediate Water (NPIW), and water ventilation processes in this region probably control the transport of iron through the intermediate water layer from the continental shelf of the Sea of Okhotsk to wide areas of the WSP. Additionally, our time series data in the Oyashio region of the WSP indicate that the pattern of seasonal changes in dissolved iron concentrations in the surface-mixed layer was similar to that of macronutrients, and that deep vertical water mixing resulted in higher winter concentrations of iron in the surface water of this region. The estimated dissolved iron supply from the iron-rich intermediate waters to the surface waters in the Oyashio region was comparable to or higher than the reported atmospheric dust iron input and thus a major source of iron to these regions. Our data suggest that the consideration of this source of iron is essential in our understanding of spring biological production and biogeochemical cycles in the western subarctic Pacific and the role of the marginal sea. - Distributions of particulate and dissolved organic and inorganic phosphorus in North Pacific surface waters
Takeshi Yoshimura, Jun Nishioka, Hiroaki Saito, Shigenobu Takeda, Atsushi Tsuda, Mark L. Wells
MARINE CHEMISTRY, 103, 1-2, 112, 121, ELSEVIER SCIENCE BV, 2007年01月, [査読有り]
英語, 研究論文(学術雑誌), Several operationally defined fractions of phosphorus (P) were measured along a surface water transect in the North Pacific. The P content in all fractions was found to increase northward from the edge of the subtropical to the subarctic region. Particulate organic P (POP) concentrations increased from 9 to 110 nmol L-1, whereas the particulate inorganic P (PIP) concentrations increased from 1 to 13 nmol L-1. A significant correlation between POP, PIP and chlorophyll a suggested that these P pools are associated directly or indirectly with phytoplankton cells. PIP comprised 10-20% of the total particulate P pool across the transect, indicating it is an important component of the marine P cycle in this region. Dissolved non-reactive P (assumed to consist predominantly of non-reactive organic P compounds, thus referred to as DOP) concentration increased from 0.10 to 0.22 mu mol L-1, whereas soluble reactive P (SRP) concentration increased from 0.01 to 1.42 mu mol L-1 along the transect. The proportion of DOP and SRP varied widely, with a large proportion of DOP in areas with low total dissolved P concentrations in lower latitudes and a large proportion of SRP in areas with high total dissolved P concentrations in higher latitudes. High demand for DOP in the lower latitudinal region would diminish the concentration of this pool relative to higher latitudinal regions where SRP is more abundant and would be preferentially utilized. The availability of SRP could have a significant impact on the concentration and probably on the composition of DOP. We show that P fractionation provides an important insight for discussing the marine P cycle. (c) 2006 Elsevier B.V. All rights reserved. - Nutrient and phytoplankton dynamics during the stationary and declining phases of a phytoplankton bloom induced by iron-enrichment in the eastern subarctic Pacific
Hiroaki Saito, Atsushi Tsuda, Yukihiro Nojiri, Jun Nishioka, Shigenobu Takeda, Hiroshi Kiyosawa, Isao Kudo, Yoshifumi Noiri, Tsuneo Ono, Yousuke Taira, Koji Suzuki, Takeshi Yoshimura, Philip W. Boyd
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 53, 20-22, 2168, 2181, PERGAMON-ELSEVIER SCIENCE LTD, 2006年, [査読有り]
英語, 研究論文(学術雑誌), This paper reports on the variations in nutrient concentrations and phytoplankton dynamics during the stationary and declining phases of a phytoplankton bloom induced by a mesoscale iron-enrichment conducted in the high-nutrient low-chlorophyll (HNLC) eastern subarctic Pacific. During the 26-d sampling period, the main pycnocline was located between 30 and 45 m with a shallow pycnocline developing at 10 m, 19 d after the first iron-enrichment. The iron-induced bloom dominated by diatoms peaked during days 15 and 18, a period of high chlorophyll a concentrations (ca. 5 mg m(-3)), and declined thereafter. Nitrogenous nutrients and phosphate were not depleted during the whole experiment. In contrast, silicic acid and iron concentrations became very low during the stationary phase of the diatom bloom (days 15-18) and F(v)/F(m) declined. These observations suggest that silicic acid and iron limitation probably prevented further development of the diatom bloom. The decline in chlorophyll a concentrations during days 19-24 was mostly due to the decrease in diatom abundance. On the other hand, cell abundances of pico- and nanophytoplankton exhibited little change until day 24. In the layer located between the main and the shallow pycnocline (10-30 m), ammonium and silicic acid concentration increased during days 19-26, suggesting recycling of these nutrients. The amount of silicic acid recycled during that period was estimated at 71.3-99.2 mmol m(-2), while the dissolution rate of biogenic silica (BSi) was estimated to be 5.9-9.2% d(-1) in the upper 50 m of the water column. These results show that the development of a shallow pycnocline during the experiment accelerated the iron and silicic acid depletion in the upper mixed layer and influenced the recycling of the organic matter assimilated during the iron-induced bloom in the ocean surface. (c) 2006 Elsevier Ltd. All rights reserved. - The uptake and export of silicon and nitrogen in HNLC waters of the NE Pacific Ocean
FA Whitney, DW Crawford, T Yoshimura
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 52, 7-8, 1055, 1067, PERGAMON-ELSEVIER SCIENCE LTD, 2005年, [査読有り]
英語, 研究論文(学術雑誌), The high-nitrate, low-chlorophyll (HNLC) waters of the Gulf of Alaska tend towards silicate rather than nitrate depletion as phytoplankton utilize nutrients during summer. This tendency is enhanced when iron supply is elevated through natural inputs such as from coastally generated rnesoscale eddies or through artificial enrichment as was carried out in an in situ experiment in July 2002. However, ship-board incubations with iron enrichment demonstrate nitrate rather than silicate depletion for these waters. The difference between in situ and in vitro experiments occurs at least in part because deck incubations do not allow export of particulate Si and N. Due to the more efficient recycling of nitrogen and carbon, export favours the removal of silicon from the upper ocean (the Si pump). Previous measurements at Ocean Station Papa (50 degrees N, 145 degrees W) show that similar to 25% of the Si, but only similar to 7% of the C and similar to 4% of the N utilized during spring growth, is exported to a depth of 200 m. These results in the Gulf of Alaska agree with the present understanding of phytoplankton controls in other HNLC regions and show that any estimates of carbon export from iron enrichment should be based on Si- rather than N-limitation. (c) 2005 Elsevier Ltd. All rights reserved. - Effect of iron enrichment on the dynamics of transparent exopolymer particles in the western subarctic Pacific
N Ramaiah, S Takeda, K Furuya, T Yoshimura, J Nishioka, T Aono, Y Nojiri, K Imai, Kudo, I, H Saito, A Tsuda
PROGRESS IN OCEANOGRAPHY, 64, 2-4, 253, 261, PERGAMON-ELSEVIER SCIENCE LTD, 2005年, [査読有り]
英語, Dynamics of transparent exopolymer particles (TEP) was studied during the first in situ iron-enrichment experiment conducted in the western subarctic Pacific in July-August 2001, with the goal of evaluating the contribution of TEP to vertical flux as a result of increased primary production following iron enrichment in open ocean ecosystems. Subsequent to the enhancement of phytoplankton production, we observed increase in TEP concentration in the surface layer and sedimentation of organic matter beneath it. Vertical profiles of TEP, chlorophyll a (Chl a) and particulate organic carbon (POC) were obtained from six depths between 5 and 70 m, from a station each located inside and outside the enriched patch. TEP and total mass flux were estimated from the floating sediment traps deployed at 200 m depth. Chl a and TEP concentrations outside the patch varied from 0.2 to 1.9 μ g L-1 and 40-60 μ g XG equiv. L-1, respectively. Inside the patch, Chl a increased drastically from day 7 reaching the peak of 19.2 μ g L-1 on day 13, which coincided with the TEP peak of 189 μ g XG equiv. L-1. TEP flux in the sediment trap increased from 41 to 88 mg XG equiv. m(-2) d(-1), with 8-14% contribution of TEP to total mass flux. This forms the basic data set on ambient concentrations of TEP in the western subarctic Pacific, and evaluation of the effect of iron enrichment on TEP. © 2005 Elsevier Ltd. All rights reserved. - The decline and fate of an iron-induced subarctic phytoplankton bloom
PW Boyd, CS Law, CS Wong, Y Nojiri, A Tsuda, M Levasseur, S Takeda, R Rivkin, PJ Harrison, R Strzepek, J Gower, RM McKay, E Abraham, M Arychuk, J Barwell-Clarke, W Crawford, D Crawford, M Hale, K Harada, K Johnson, H Kiyosawa, Kudo, I, A Marchetti, W Miller, J Needoba, J Nishioka, H Ogawa, J Page, M Robert, H Saito, A Sastri, N Sherry, T Soutar, N Sutherland, Y Taira, F Whitney, SKE Wong, T Yoshimura
NATURE, 428, 6982, 549, 553, NATURE PUBLISHING GROUP, 2004年04月, [査読有り]
英語, 研究論文(学術雑誌), Iron supply has a key role in stimulating phytoplankton blooms in high-nitrate low-chlorophyll oceanic waters(1-5). However, the fate of the carbon fixed by these blooms, and how efficiently it is exported into the ocean's interior, remains largely unknown(1-5). Here we report on the decline and fate of an iron-stimulated diatom bloom in the Gulf of Alaska. The bloom terminated on day 18, following the depletion of iron and then silicic acid, after which mixed-layer particulate organic carbon (POC) concentrations declined over six days. Increased particulate silica export via sinking diatoms was recorded in sediment traps at depths between 50 and 125 m from day 21, yet increased POC export was not evident until day 24. Only a small proportion of the mixed-layer POC was intercepted by the traps, with more than half of the mixed-layer POC deficit attributable to bacterial remineralization and mesozooplankton grazing. The depletion of silicic acid and the inefficient transfer of iron-increased POC below the permanent thermocline have major implications both for the biogeochemical interpretation of times of greater iron supply in the geological past(6,7), and also for proposed geo-engineering schemes to increase oceanic carbon sequestration(3,8). - Size-fractionated iron distributions and iron-limitation processes in the subarctic NW Pacific
Jun Nishioka, Shigenobu Takeda, Isao Kudo, Daisuke Tsumune, Takeshi Yoshimura, Kenshi Kuma, Atsushi Tsuda
GEOPHYSICAL RESEARCH LETTERS, 30, 14, AMER GEOPHYSICAL UNION, 2003年07月, [査読有り]
英語, 研究論文(学術雑誌), Comparison of vertical profiles of size-fractionated iron between the western and eastern subarctic North Pacific clearly showed higher labile particulate iron concentrations towards the west and this result strongly supports the higher iron supply in the western region. Additionally, the results of the SEEDS experiment, the first meso-scale iron enrichment experiment in the subarctic North Pacific, clearly showed that artificially enriched iron in the dissolved fraction (mainly in colloidal fraction) was rapidly transformed to suspended labile particulate iron during phytoplankton growth and was retained in the surface mixed layer. Probably, this same rapid transformation process occurs naturally after sporadic atmospheric iron supply and the labile particulate iron is retained in the western region. Furthermore, this transformation process reduces dissolved concentration of iron and its bioavailability. Therefore, the transformation process is important for understanding how phytoplankton became iron limited and the biogeochemical iron cycle in the western subarctic North Pacific. - A mesoscale iron enrichment in the western Subarctic Pacific induces a large centric diatom bloom
A Tsuda, S Takeda, H Saito, J Nishioka, Y Nojiri, Kudo, I, H Kiyosawa, A Shiomoto, K Imai, T Ono, A Shimamoto, D Tsumune, T Yoshimura, T Aono, A Hinuma, M Kinugasa, K Suzuki, Y Sohrin, Y Noiri, H Tani, Y Deguchi, N Tsurushima, H Ogawa, K Fukami, K Kuma, T Saino
SCIENCE, 300, 5621, 958, 961, AMER ASSOC ADVANCEMENT SCIENCE, 2003年05月, [査読有り]
英語, 研究論文(学術雑誌), We have performed an in situ test of the iron limitation hypothesis in the subarctic North Pacific Ocean. A single enrichment of dissolved iron caused a large increase in phytoplankton standing stock and decreases in macronutrients and dissolved carbon dioxide. The dominant phytoplankton species shifted after the iron addition from pennate diatoms to a centric diatom, Chaetoceros debilis, that showed a very high growth rate, 2.6 doublings per day. We conclude that the bioavailability of iron regulates the magnitude of the phytoplankton biomass and the key phytoplankton species that determine the biogeochemical sensitivity to iron supply of high-nitrate, low-chlorophyll waters. - Seasonal variations in nutrients and a factor limiting phytoplankton growth in Lake Ohnuma
Takeshi Yoshimura, Isao Kudo
Japanese Journal of Limnology, 62, 3, 205, 217, Japanese Society of Limnology, 2001年, [査読有り]
日本語, 研究論文(学術雑誌), Nutrient concentrations and alkaline phosphatase activity were measured in Lake Ohnuma from June 1995 to March 1997. Sufficient amounts of dissolved inorganic nitrogen remained throughout the year, whereas soluble reactive phosphorus concentrations were extremely low (close to the detection limit). The N:P ratio in the standing stock of nutrients ranged 74-3000 (by atoms), far richer in nitrogen than the Redfield ratio. Chlorophyll a concentrations were high (5-22 μ g L-1) during icefree seasons, but the elemental composition of phytoplankton exhibited a moderate phosphorus deficiency. A high seasonal variability in alkaline phosphatase activity suggested that not only was phytoplankton growth highly limited by phosphorus, but that the degree of this limitation varied. Alkaline phosphatase activity served as a simple and an effective indicator for judging the phosphorus status of phytoplankton. - Exhaustion of nitrate terminates a phytoplankton bloom in Funka Bay, Japan: change in SiO4,: NO3 consumption rate during the bloom
Kudo, I, T Yoshimura, M Yanada, K Matsunaga
MARINE ECOLOGY PROGRESS SERIES, 193, 45, 51, INTER-RESEARCH, 2000年, [査読有り]
英語, 研究論文(学術雑誌), Time series observations over 36 h were carried out in Funka Bay, Japan, to complement a 6 yr nutrient-dynamics study during a diatom spring bloom, especially focusing on nitrate and silicate as limiting nutrients. At the beginning of the study, nutrient concentrations were high (NO3 = 10 mu M, SiO4 = 20 mu M and PO4 = 0.8 mu M) Nitrate was depleted first during the night while silicate and phosphate remained at 10 and 0.3 mu M, respectively. High nitrate reductase activity was observed the following morning, indicating the assimilation of nitrate in situ by the dominant phytoplankter, Chaetoceros socialis. The consumption ratio of SiO4:NO3 increased by 3 times from 0.83 before the peak to 2.5 after the peak of the bloom. This change was also obvious in the elemental composition of phytoplankton, showing an increase in Si:C and Si:N ratios by a factor of 2 to 4 after the peak of the bloom, while C:N ratio remained constant at around 7. As a consequence of this change in the consumption rate of SiO4 and NO3, both silicate and nitrate were finally depleted in the photic zone. - Change in the water quality in Lake Ohnuma, Hokkaido, Japan: A comparison of 1977 and 1996
Takeshi Yoshimura, Isao Kudo, Mitsuru Yanada, Katsuhiko Matsunaga
Limnology, 1, 1, 63, 68, Springer Japan, 2000年, [査読有り]
英語, 研究論文(学術雑誌), Annual variations in nutrients, algal biomass, and primary production were investigated in Lake Ohnuma, Japan, in 1996 in order to compare them with 1977. Chlorophyll a concentrations gradually increased after the ice melted and reached a maximal value of 20.7 μg1-1 in August. Phosphate concentrations in the lake were close to the detection limit throughout the study period, whereas sufficient nitrate remained even in the productive summer season. In contrast, in the summer of 1977, both nutrients were exhausted, and primary production was less than 0.2g Cm-2 day-1. Primary production in 1996 ranged from 0.4 to 5.8g Cm-2 day-1, which was 2 to 30 times higher than 20 years ago. These results indicate that the lake has become eutrophic in the last two decades. A comparison of the nutrients in the inflowing river between 1977 and 1996 indicated that nitrate and ammonium concentrations were markedly elevated in the rivers, and therefore the nitrogen loading to the lake tripled.
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日本語 - 珪藻の栄養状態によってCO2の変化が生元素動態に及ぼす影響は変わる
杉江恒二, 芳村毅, 脇田昌英, 日本海洋学会大会講演要旨集, 2017, 2017年 - 水温およびCO2分圧の増加に対する夏季の日本海沿岸域の植物プランクトン群集組成の変化
芳村毅, 野尻幸宏, 大森裕子, 谷本浩志, 清沢弘志, 吉川貴志, 日本海洋学会大会講演要旨集, 2017, 2017年 - 福島第一原子力発電所事故による放出した放射性物質の沿岸域における5年間の挙動
津旨大輔, 坪野考樹, 芳村毅, 三角和弘, 立田穣, 青山道夫, アイソトープ・放射線研究発表会要旨集, 53rd, 53, 2016年06月20日
日本語 - 夏季の日本海沿岸域のプランクトン群集に対する水温およびCO2分圧の増加の影響評価
芳村毅, 野尻幸宏, 野尻幸宏, 大森裕子, 大森裕子, 谷本浩志, 清沢弘志, 吉川貴志, 日本海洋学会大会講演要旨集, 2016, 2016年 - 東電福島第一原発事故起源の放射性セシウムの日本沿岸での長期挙動と微細構造
青山道夫, 芳村毅, 津旨大輔, 浜島靖典, 日本海洋学会大会講演要旨集, 2016, 2016年 - 海洋酸性化と温暖化の複合ストレスに因る亜寒帯北太平洋のプランクトン群集動態の変化
杉江恒二, 亀山宗彦, 芳村毅, 内田裕, 西岡純, 原田尚美, 村田昌彦, 日本海洋学会大会講演要旨集, 2016, 2016年 - 福島県沿岸での海水及び魚筋肉中放射性セシウムとトリチウム濃度
青山道夫, 石丸隆, 立田穣, 津旨大輔, 芳村毅, 浜島靖典, 玉利俊哉, 水野拓治, 日本海洋学会大会講演要旨集, 2015, 2015年 - 沿岸域のプランクトン群集に対する水温およびCO2分圧の増加の影響評価
芳村毅, 野尻幸宏, 堀田公明, 日本海洋学会大会講演要旨集, 2015, 2015年 - 福島県および茨城県沿岸での海水及び魚筋肉中の放射性セシウムとトリチウム濃度
青山道夫, 浜島靖典, 立田穣, 津旨大輔, 芳村毅, 石丸隆, 玉利俊哉, 水野拓治, アイソトープ・放射線研究発表会要旨集, 52nd, 2015年 - 二酸化炭素分圧の変化が春季親潮域における珪藻rbcL遺伝子の発現と系統組成に与える影響
遠藤寿, 遠藤寿, 鈴木光次, 鈴木光次, 杉江恒二, 杉江恒二, 芳村毅, 日本海洋学会大会講演要旨集, 2015, 2015年 - 海洋酸性化に対する生物応答の解明(その3)―複数種の植物プランクトン単離株による培養実験―
芳村毅, 杉江恒二, 電力中央研究所環境科学研究所研究報告, V13017, 19P, 3,1-13, 2014年04月
電力中央研究所, 日本語 - 海洋酸性化に対する生物応答の解明(その4)―複数地点の外洋域プランクトン群集による培養実験―
芳村毅, 杉江恒二, 鈴木光次, 西岡純, 電力中央研究所環境科学研究所研究報告, V13018, 25P, 3,1-19, 2014年04月
電力中央研究所, 日本語 - 円石藻類の増殖に対する水温およびCO2分圧の増加の影響評価
芳村毅, 野尻幸宏, 日本海洋学会大会講演要旨集, 2014, 2014年 - 東電福島第一原子力発電所事故で海洋に放出された核分裂生成物および中性子放射化生成物の挙動
青山道夫, 浜島靖典, 芳村毅, 本多牧生, 喜多村稔, HULT Mikael, 日本地球化学会年会講演要旨集, 60th, 2013年 - 植物プランクトンの増殖に対するCO2分圧増加の影響の評価
芳村毅, 杉江恒二, 杉江恒二, 津旨大輔, 日本海洋学会大会講演要旨集, 2013, 2013年 - rbcL遺伝子からみた春季親潮域の珪藻類に対するCO2の効果
遠藤寿, 鈴木光次, 杉江恒二, 杉江恒二, 芳村毅, 日本海洋学会大会講演要旨集, 2013, 2013年 - 微細藻類によるバイオ燃料生産のための基礎的検討(その2)―炭化水素生産藻Botryococcus braunii Showa株の増殖モデルの構築―
本多正樹, 芳村毅, 岡田茂, 電力中央研究所環境科学研究所研究報告, V11021, 19P, 13,巻頭1-3, 2012年06月
電力中央研究所, 日本語 - 微細藻類によるバイオ燃料生産のための基礎的検討(その1)―炭化水素生産藻Botryococcus braunii Showa株の安定的培養法の確立と増殖特性の把握―
芳村毅, 本多正樹, 岡田茂, 電力中央研究所環境科学研究所研究報告, V11013, 22P, 16,巻頭1-3, 2012年06月
電力中央研究所, 日本語 - 海洋酸性化に対する生物応答の解明(その2)―単離植物プランクトン株を用いた培養試験―
杉江恒二, 芳村毅, 電力中央研究所環境科学研究所研究報告, V11049, 19P, 13,巻頭1-3, 2012年05月
電力中央研究所, 日本語 - 海洋酸性化に対する生物応答の解明(その2)単離植物プランクトン株を用いた培養試験
杉江 恒二, 芳村 毅, 電力中央研究所報告. 研究報告. 電力中央研究所環境科学研究所 編, 0, 11049, 1, 13,巻頭1-3, 2012年05月
電力中央研究所, 日本語 - 潮汐混合とオホーツク海・ベーリング海の物理・化学・生物過程―白鳳丸KH09‐4航海・おしょろ丸・クロモフ2006/2007シンセシス―pCO2と鉄濃度の違いが夏季ベーリング海の植物プランクトン群集によるSiとNの利用比に与える影響
杉江恒二, 遠藤寿, 鈴木光次, 芳村毅, 月刊海洋, 43, 12, 735, 741, 2011年12月01日
日本語 - Oceanic iron supply mechanisms which support the spring diatom bloom in the Oyashio region, western subarctic Pacific (vol 116, C04007, 2011)
Jun Nishioka, Tsuneo Ono, Hiroaki Saito, Keiichiro Sakaoka, Takeshi Yoshimura, JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS, 116, 2011年04月, [査読有り]
AMER GEOPHYSICAL UNION, 英語, その他 - 観測されたSF6濃度をデータ同化する拡散モデルと水塊追跡への適用
坪野考樹, 津旨大輔, 芳村毅, 西岡純, 水工学論文集(CD-ROM), 55, ROMBUNNO.204, 2011年02月22日
日本語 - Fe*を用いた親潮域中層におけるオホーツク海からの鉄移送量の評価
西岡純, 小埜恒夫, 齊藤宏明, 坂岡桂一郎, 芳村毅, 日本海洋学会大会講演要旨集, 2010, 73, 2010年08月25日
日本語 - 観測されたSF6濃度を用いたグリーン関数による拡散モデルの制御変数の推定
坪野考樹, 津旨大輔, 芳村毅, 西岡純, 水工学論文集(CD-ROM), 54, ROMBUNNO.189, 2010年02月22日
日本語 - 海洋酸性化に対する生物応答の解明―プランクトン群集を用いた培養試験―
芳村毅, 杉江恒二, 西岡純, 電力中央研究所環境科学研究所研究報告, V09005, 21P, 15,巻頭1〜3, 2009年11月
電力中央研究所, 日本語 - グリーン関数法でSF6濃度結果を拡散方程式にデータ同化する手法を用いた水塊移動の推定
坪野考樹, 津旨大輔, 芳村毅, 西岡純, 水工学論文集(CD-ROM), 53, ROMBUNNO.249, 2009年02月22日
日本語 - 大気中CO2濃度の増加がもたらす海洋酸性化―海洋プランクトンを通じてCO2吸収に与える影響に関する文献調査―
芳村毅, 津旨大輔, 電力中央研究所環境科学研究所研究報告, V06019, 27P, 21,巻頭1〜4, 2007年05月
電力中央研究所, 日本語 - 鉄の効果に着目した海洋中炭素吸収量の変化予測
津旨大輔, 仲敷憲和, 吉田義勝, 芳村毅, BRYAN Frank O, LINDSAY Keith, MOORE Keith J, DONEY Scott C, 電力中央研究所環境科学研究所研究報告, V06010, 20P, 14,巻頭1〜4, 2007年04月
電力中央研究所, 日本語 - 海洋への鉄散布による生物的CO2固定(その2)―鉄散布実験における水塊追跡手法の改良―
津旨大輔, 芳村毅, 西岡純, 電力中央研究所環境科学研究所研究報告, V05026, 19P, 16,巻頭1〜3, 2006年08月
電力中央研究所, 日本語 - 海域毎の鉄散布に対する植物プランクトン増殖の応答性の違いとその要因の検討
西岡純, 津旨大輔, 芳村毅, 電力中央研究所環境科学研究所研究報告, V04033, 24P, 22,巻頭1〜4, 2005年06月
電力中央研究所, 日本語 - 北太平洋における海洋鉄散布による生物的炭素固定量の評価
芳村毅, 西岡純, 津旨大輔, 電力中央研究所環境科学研究所研究報告, V04020, 17P, 12,巻頭1〜3, 2005年06月
電力中央研究所, 日本語 - 親潮海域春季ブルーム期における亜酸化窒素の挙動
今村正裕, 西岡純, 芳村毅, 斉藤宏明, 小埜恒夫, 日本海洋学会大会講演要旨集, 2005, 2005年 - 北西部北太平洋亜寒帯と親潮域における鉄,カドミウム及び栄養塩の挙動
高田兵衛, 久万健志, 西岡純, 芳村毅, 緑川貴, 木村完, 山口篤, 高木省吾, 坂岡桂一郎, 日本海洋学会大会講演要旨集, 2005, 149, 2005年
日本語 - 西部北太平洋での鉄散布実験における溶存有機物の挙動解析
芳村毅, 西岡純, 電力中央研究所環境科学研究所研究報告, V04002 (Web), 2004年 - 海洋の生物的炭素固定における溶存有機物の役割に関する文献調査
芳村毅, 西岡純, 電力中央研究所我孫子研究所報告, U03004, 20P, 15,巻頭1〜4, 2003年05月
電力中央研究所, 日本語 - 噴火湾における河川からの栄養塩負荷量およびそれらが湾内の基礎生産に与える影響
芳村 毅, 工藤 勲, Takasi Yoshimura, Isao Kudo, Graduate Scholl of Fisheries Science Hokkaido University, Graduate Scholl of Fisheries Science Hokkaido University, 海の研究 = Umi no Kenkyu (Oceanography in Japan), 12, 2, 185, 193, 2003年03月05日
河川から噴火湾への栄養塩負荷量を明らかにするために,噴火湾に流人する8河川および発電所排水路1か所において,流量および栄養塩濃度を一年にわたり観測した。その結果,河川流量は雪解け時の4月に特異的に高い明瞭な季節変化を示した。また,河川水中の栄養塩濃度は河川によって大きく異なったが,全河川の加重平均濃度は硝酸塩が24μM,アンモニウム塩が2.9μM,リン酸塩が0.30μM,ケイ酸塩が270μMであり. DIN:DIP比が非常に高いことが明らかとなった。河川から噴火湾への一年間の淡水負荷量は2.1×10^9m^3であり,溶存無機窒素,リン酸塩,ケイ酸塩負荷量はそれぞれ57×l0^6mol,0.6.3×10^6mol. 570x10^6molだった。これらの栄養塩負荷量が噴火湾の基礎生産に与える影響は総生産を考慮した場合は非常に小さなものだった。しかしながら,新生産を夏期のリン酸塩の減少量から見積もった結果,河川由来の栄養塩が噴火湾の夏期の新生産の最大10%を支えていることが明らかとなった。Water discharge and milrient concentrations were investigated in eight, rivers and a power plant drainage in order to reveal the nutrient, loadings through rivers into Funka Bay. The river water discharge changed seasonally with a remarkable peak in April. The volume-weighted mean concentrations for all rivers of nitrate, ammonium, phosphate and silicate were 24μM, 2.9μM, 0.30μM, and 270μM, respectively. Annual nutrient loadings of dissolved inorganic nitrogen, phosphate and silicate were 57x10^9mol 0.63×l0^6mol, and 570×l0^6mol, respectively. The river waters had higher DIN:DIP ratios than surface waters in Funka Bay, indicating that the former was highly enriched in nitrogen compared with phosphorus. The contributions of riverine nutrient, loadings to primary production in Funka Bay were only 1% when total annual production was considered. In terms of newproduction, however, riverine nutrient, loadings seem to support a significant part. (10%) of summer new production in Funka Bay., 日本海洋学会, 日本語 - 海洋への鉄散布による生物的CO2固定―鉄散布手法および観測手法と海洋プランクトン生態系の応答
西田純, 津旨大輔, 芳村毅, 武田重信, 津田敦, 電力中央研究所我孫子研究所報告, U02017, 20P, 2003年01月
日本語
所属学協会
Works(作品等)
- 北海道サロマ湖でのホタテガイ養殖を支援する環境調査
LASBOS Moodle, 芳村毅, 2020年08月15日 - 現在
共同研究・競争的資金等の研究課題
- 海洋物質循環の基準値であるレッドフィールド比を再考する
科学研究費助成事業
2021年04月01日 - 2024年03月31日
芳村 毅, 工藤 勲, 今井 圭理
海洋によるCO2の吸収・固定量は植物プランクトンによる炭素と栄養素の利用比率によって変化します。その比率としてレッドフィールド比が良く知られており,気候変化を予測する生態系モデルにおいて固定値として使われていますが,実測データの蓄積は十分ではありません。また,プランクトン群集はサイズの異なる多くの種で形成されますが,それらをひとまとめにした比率しか議論されてきませんでした。海洋環境の変化によりプランクトン群集組成が変化すると予測されていますが,その変化が生態系に与える影響を評価できません。そこで,本研究は有機物粒子のサイズ分画手法を確立したうえで,各サイズ画分の主要4元素組成比(炭素:窒素:リン:ケイ素)に関するデータを取得します。
研究一年目の2021年度は海水中の有機物粒子をサイズ毎に分画するための手法を検討しました。撹拌式セルを用いた限外ろ過システムを応用した「限外ろ過方式」と,孔径の異なるフィルターに連続的に通過させる「逐次ろ過方式」を並行して検討しました。その結果,限外ろ過方式はフィルター上に粒子が捕捉されてしまい,有機物粒子量を過小評価することがわかりました。有機物粒子のサイズ分画手法としては逐次ろ過方式が適していることがわかりました。逐次ろ過方式はリンおよびケイ素の分析試料を作成するのに適していますが,炭素および窒素を分析するためにはフィルター上に集めた有機物粒子を液体に懸濁させて回収する必要があります。フィルターに付着する有機物粒子を完全に回収する手法を検討した結果,超音波洗浄を利用することで達成できることがわかりました。これらの検討により,孔径の異なるメンブレンフィルターを用いた逐次ろ過方式によって有機物粒子を三つにサイズ分画し,超音波洗浄を活用して回収した有機物粒子の主要4元素を分析することで,各サイズ画分の元素組成比を分析するための道筋が完成しました。
日本学術振興会, 基盤研究(C), 北海道大学, 21K12219 - ドローンと船舶の同時観測で明らかにする河川水が沿岸域で駆動する物質循環
科学研究費助成事業
2021年04月01日 - 2024年03月31日
木田 新一郎, 田中 潔, 芳村 毅, 伊佐田 智規
日本学術振興会, 基盤研究(B), 九州大学, 21H03591 - 陸奥湾における持続的な二枚貝養殖のための貧栄養化のメカニズム解明と対策提言
科学研究費助成事業
2018年04月01日 - 2021年03月31日
工藤 勲, 芳村 毅
ホタテガイ垂下養殖漁業が行われている青森県陸奥湾で、近年栄養塩濃度が減少する「貧栄養化」が起こっていることが明らかとなった。本研究は、持続的な生物生産活動の維持を脅かしかねない海域の「貧栄養化」のメカニズムを解明し、貧栄養化の効果的な方策を提言することを目的として行われた。夏季に栄養塩は枯渇状態にあり、この時期の基礎生産(ホタテガイの餌生産)は栄養塩が不足していた。 海底耕耘により海底に埋没した栄養塩の回帰効果があることが確認され、その量は海水中に存在する栄養塩の10-20%に相当すると見積もられた。このことから「貧栄養化」の対策として海底耕耘は有効な手段であることが明らかになった。
日本学術振興会, 基盤研究(C), 北海道大学, 18K05776 - 海洋生態系における放射性物質の移行・濃縮状況の把握
科学研究費助成事業
2012年06月28日 - 2017年03月31日
神田 穣太, 喜多村 稔, 西川 淳, 青野 辰雄, 山口 篤, 高木 省吾, 土屋 光太郎, 立田 穣, 林 敏史, 野田 明, 渡辺 豊, 茂木 正人, 田中 祐志, 守屋 繁春, 小林 卓也, 芳村 毅, 石丸 隆, 五十嵐 敏, 須賀 次郎, 山川 紘, 大津 秀暁, 伊藤 友加里, 高澤 伸江, 内山 香織, 久保 篤史, 今井 圭理
海洋生態系における福島第一原子力発電所事故由来の放射性物質について、以下の研究を行った。1)福島沿岸域において生物と環境の放射性セシウムについて、年に2回の船舶観測による経時的なデータセットを得た。また外洋域の動物プランクトンについても複数の海域で時系列データを得た。2)岩礁性の底生魚に重点を置いて、生態系への移行経路を検討した結果、懸濁・沈降粒子や堆積物の有機物画分等からの移行は小さいことを確認した。3)現場観測データを用い、生態系内の放射性セシウム推移をモデルにより再現し、海洋生物の放射能レベルの今後の推移について検討した。
日本学術振興会, 新学術領域研究(研究領域提案型), 東京海洋大学, 24110005 - 比較可能性がとれた海水中栄養塩濃度の全球分布及び総量に関する研究
科学研究費助成事業
2011年05月31日 - 2014年03月31日
青山 道夫, 村田 昌彦, 芳村 毅, 日置 昭治, 内田 裕, ハイド デイビット, ディクソン アンドリュー, 小杉 如央
海洋での栄養塩の正確な空間分布を明らかにするための基礎となるデータセット作成を行った。栄養塩標準を用い分析の不確かさが0.2%より良い測点間の比較可能性が確保されている航海を6航海行うとともに複数のデータセットから栄養塩データが存在している航海を抽出した。これらのデータから、栄養塩標準を使用し比較可能性が明示的に確保されている航海を基準として、全球243点で交点での解析をおこない栄養塩濃度を補正した後、さらに当初の予定にはなかった溶存酸素量のデータについても栄養塩データを同様な処理を行い、深度面で整合性のある0.5度メッシュ、平均50m厚み136層の格子点データセットを作成した。
日本学術振興会, 基盤研究(S), 23221003 - 北太平洋亜寒帯海域の表層酸性化が炭酸カルシウム殻のプランクトンにおよぼす影響予測
科学研究費助成事業
2011年 - 2013年
服部 寛, 佐々木 洋, 芳村 毅, 小埜 恒夫
地球温暖化が原因となる海洋酸性化が植物プランクトン、特に炭酸カルシウム殻をもつ円石藻類に対する影響を調べるための酸性化予測実験を行った。現場における分布密度の把握と酸性化予測実験の試料分析は全て終わっていないが、これまでの結果では現存量が多い円石藻類の種ほど酸性化の影響を受けると推定出来る結果を得ていて、現在この原因の理論的背景を構築している段階である。
北太平洋と比較を目的に南極海で酸性化実験を実施しする機会に恵まれ、現在はその試料の解析も行なっている。南極海では植物プランクトン現存量で優先する珪藻類が多いことから、円石藻類の応答に加え、珪藻類にも着目して、試料の分析を勧めている。
日本学術振興会, 基盤研究(C), 東海大学, 23510013 - 海水中のCO2濃度の増加が植物プランクトンの増殖と有機物生産に与える影響の解明
科学研究費助成事業
2010年 - 2012年
芳村 毅
単離株を用いた室内培養実験と現場群集を用いた船上培養実験により,CO2分圧の上昇が植物プランクトンの比増殖速度と元素組成比や有機物生産に与える影響を調査した。CO2分圧の上昇に対する応答は種特異的であり,水温・光量の条件によっても変化することが明らかとなった。現場群集への影響は顕著ではないが,CO2増加に対する明確なトレンドを示すケースがあった。CO2分圧の上昇は実海域での植物プランクトンの動態を変化させる要因となり得ることがわかった。
日本学術振興会, 若手研究(A), Central Research Institute of Electric Power Industry, 22681004 - 北太平洋亜寒帯域の鉄濃度が生物生産と二酸化炭素収支に及ぼす影響に関する観測研究
科学研究費助成事業
2004年 - 2006年
小埜 恒夫, 葛西 広海, 西岡 純, 渡辺 豊, 芳村 毅
通常のCTDを利用したクリーン採水技術を確立し、親潮域および混合域において年数回の鉄の反復断面観測を実施した。世界で初めて栄養塩と同一の時空間分解能で得られた、鉄濃度周年変動データの解析から、以下の事があきらかになった。
1]親潮域表層における溶存鉄の各月の海域平均濃度は、主要栄養塩と定性的に同じ季節変動パターンを示すだけでなく、硝酸と定量的に一定比(ΔDFe/ΔN=27x10^<-6>[mol/mol])を保って増減している。表層鉄濃度のピークは冬期の最大混合深度期(1-3月)であり、黄砂飛来時期(4月)とは明瞭に異なる。
2]親潮表層の冬期溶存鉄濃度はアラスカ湾より遙かに高いが、両者の亜表層域の鉄濃度も同様に異なる為に、両海域の冬期表層鉄濃度は、亜表層からのエントレインメントと鉛直渦拡散を仮定した同じモデルで再現可能である。親潮表層の溶存鉄:硝酸費も同様にアラスカ湾より高く、北太平洋中層水(NPIW)のそれと同一値をしめす。
3]これらの事から、西部北太平洋表層に存在する鉄は数年-数十年程度の平均滞留時間を持っており、したがって周年-経年変動のスケールでは、鉄の動態は主要栄養塩と同様であることが推定される。つまり大気ダストからの鉄の供給は従来の定説に反してそれほど重要ではなく、生物による取り込みと中層での再分解、そして鉛直混合による表層への回帰という海洋内部での再循環だけで、ほぼ加不足なく溶存鉄の周年変動は説明出来る。
4]春季ブルーム終了時の表層溶存鉄濃度がほぼ0.25nMで一定となる事から、ブルーム形成ケイ藻種の成育限界鉄濃度が0.25nM程度と高く、このためブルーム期には硝酸でなく鉄が主な制限栄養素になっている事が推定される。一方周年を通じた硝酸・溶存鉄プロットは鉄側に切片を持つ事から、鉄も硝酸も低濃度となる夏期には硝酸が親潮表層の制限栄養素となっている。
日本学術振興会, 基盤研究(B), 独立行政法人水産総合研究センター, 16310019