Oct. 2018, 伊藤太郎学術賞　　　　　　　　　　　　　　　

Oct. 2017, 斎藤眞賞　　　　　　　　　　　　　　　
寳金 清博

Feb. 2013, 公益信託美原脳血管障害研究振興基金, 美原賞　　　　　　　　　　　　　　　
宝金 清博

Sep. 2007, 北海道医師会賞・北海道知事賞　　　　　　　　　　　　　　　
寳金 清博

Apr. 1999, 日本脳卒中の外科学会賞（鈴木賞）　　　　　　　　　　　　　　　

Dec. 1993, 第22回かなえ医学奨励賞　　　　　　　　　　　　　　　

A feasibility pilot study comparing tablets and smartphones for an app-based speed training program in older people: an open-label, randomized controlled PROBE trial
Yuki Takakura, Mika Otsuki, Ryo Takagi, Kiyohiro Houkin
Cognitive Processing, Springer Science and Business Media LLC, 06 Mar. 2025
Scientific journal

Intracerebral transplantation of MRI-trackable autologous bone marrow stromal cells for patients with subacute ischemic stroke.
Masahito Kawabori, Satoshi Kuroda, Hideo Shichinohe, Kaoru Kahata, Souichi Shiratori, Satoshi Ikeda, Taisuke Harada, Kenji Hirata, Khin Khin Tha, Masato Aragaki, Shunsuke Terasaka, Yoichi M Ito, Naoki Nishimoto, Shunsuke Ohnishi, Ichiro Yabe, Kohsuke Kudo, Kiyohiro Houkin, Miki Fujimura
Med (New York, N.Y.), 18 Mar. 2024, [International Magazine]
English, Scientific journal, BACKGROUND: Ischemic stroke is one of the leading causes of death and neurological disability worldwide, and stem cell therapy is highly expected to reverse the sequelae. This phase 1/2, first-in-human study evaluated the safety, feasibility, and monitoring of an intracerebral-transplanted magnetic resonance imaging (MRI)-trackable autologous bone marrow stromal cell (HUNS001-01) for patients with subacute ischemic stroke. METHODS: The study included adults with severe disability due to ischemic stroke. HUNS001-01 cultured with human platelet lysates and labeled with superparamagnetic iron oxide was stereotactically transplanted into the peri-infarct area 47-64 days after ischemic stroke onset (dose: 2 or 5 × 107 cells). Neurological and radiographic evaluations were performed throughout 1 year after cell transplantation. The trial was registered at UMIN Clinical Trial Registry (number UMIN000026130). FINDINGS: All seven patients who met the inclusion criteria successfully achieved cell expansion, underwent intracerebral transplantation, and completed 1 year of follow-up. No product-related adverse events were observed. The median National Institutes of Health Stroke Scale and modified Rankin scale scores before transplantation were 13 and 4, which showed improvements of 1-8 and 0-2, respectively. Cell tracking proved that the engrafted cells migrated toward the infarction border area 1-6 months after transplantation, and the quantitative susceptibility mapping revealed that cell signals at the migrated area constantly increased throughout the follow-up period up to 34% of that of the initial transplanted site. CONCLUSIONS: Intracerebral transplantation of HUNS001-01 was safe and well tolerated. Cell tracking shed light on the therapeutic mechanisms of intracerebral transplantation. FUNDING: This work was supported by the Japan Agency for Medical Research and Development (AMED; JP17bk0104045 and JP20bk0104011).

Posterior cerebral artery involvement in unilateral moyamoya disease is exclusively ipsilateral and influenced by RNF213 mutation gene dose: The SUPRA Japan study: PCA involvement in unilateral moyamoya.
Yohei Mineharu, Yasushi Takagi, Akio Koizumi, Takaaki Morimoto, Takeshi Funaki, Tomohito Hishikawa, Yoshio Araki, Hitoshi Hasegawa, Jun C Takahashi, Satoshi Kuroda, Kiyohiro Houkin, Susumu Miyamoto
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 33, 2, 107513, 107513, Feb. 2024, [International Magazine]
English, Scientific journal, OBJECTIVES: The characteristics and clinical implications of posterior cerebral artery (PCA) involvement in unilateral moyamoya disease (U-MMD), such as laterality, frequency of the RNF213 p.R4810K mutation, and clinical outcomes, have not been well studied. POPULATION AND METHODS: We analyzed a cohort of 93 patients with U-MMD who participated in the SUPRA Japan study. Clinical characteristics and radiological examinations were collected from medical records. The presence of the p.R4810K mutation was determined using a TaqMan assay. The clinical outcome was assessed using the modified Rankin Scale (mRS). Univariate and multivariate logistic regression analyses were performed to assess the associations. RESULTS: Among the patients with U-MMD, PCA involvement was observed in 60.0 % (3/5) of patients with homozygous mutation, 11.3 % (7/62) of those with heterozygous mutation, and 3.8 % (1/26) of those with wild type, showing a significant linear trend (p < 0.001 for trend). PCA involvement was observed exclusively on the same side as the affected anterior circulation. Dyslipidemia and cerebral infarction at initial onset were independently associated with mRS ≥1. Hypertension was associated with mRS ≥1 and it was also linked to infarction at initial onset, suggesting a potential confounding effect. Although PCA involvement showed a trend for higher mRS, it was not statistically significant. CONCLUSIONS: Our findings indicate a gene dose effect of the p.R4810K mutation on PCA involvement, with the homozygous state showing the most significant effect. Both genetic and modifiable factors such as dyslipidemia may influence the progression of U-MMD.

Allogeneic Stem Cell Therapy for Acute Ischemic Stroke
Kiyohiro Houkin, Toshiya Osanai, Shinichiro Uchiyama, Kazuo Minematsu, Akihiko Taguchi, Katsuhiko Maruichi, Yoshimasa Niiya, Katsuyuki Asaoka, Yoshihiro Kuga, Katsumi Takizawa, Koichi Haraguchi, Shinichi Yoshimura, Kazumi Kimura, Koji Tokunaga, Atsuo Aoyama, Fusao Ikawa, Chikanori Inenaga, Tatsuya Abe, Atsushi Tominaga, Shinichi Takahashi, Kohsuke Kudo, Miki Fujimura, Taku Sugiyama, Masaki Ito, Masahito Kawabori, David C. Hess, Sean I. Savitz, Teruyuki Hirano, Kiyohiro Houkin, Toshiya Osanai, Katsuhiko Maruichi, Yoshimasa Niiya, Katsuyuki Asaoka, Katsumi Takizawa, Kouichi Haraguchi, Rokuya Tanikawa, Akira Tempaku, Yusuke Shimoda, Masanori Isobe, Kenji Kamiyama, Masafumi Ohtaki, Norihito Shimamura, Junta Moroi, Aiki Marushima, Shinichi Takahashi, Takao Urabe, Teruyuki Hirano, Kazumi Kimura, Kazuo Kitagawa, Hidetoshi Kasuya, Yoshikane Izawa, Yasuyuki Iguchi, Koichi Oki, Koichi Kato, Yoshihisa Yamano, Satoshi Kuroda, Atsushi Sato, Chikanori Inenaga, Keizo Yasui, Kazunori Toyoda, Shinichi Yoshimura, Nobuyuki Sakai, Yoshihiro Kuga, Atsuo Aoyama, Fusao Ikawa, Koji Tokunaga, Atsushi Tominaga, Yasushi Takagi, Masahiro Yasaka, Tatsuya Abe, Takayuki Matsuo, Toshiro Yonehara, Tadashi Terasaki, Hideki Matsuoka
JAMA Neurology, American Medical Association (AMA), 16 Jan. 2024
Scientific journal, Importance

Cell therapy is a promising treatment approach for stroke and other diseases. However, it is unknown whether MultiStem (HLCM051), a bone marrow–derived, allogeneic, multipotent adult progenitor cell product, has the potential to treat ischemic stroke.

Objective

To assess the efficacy and safety of MultiStem when administered within 18 to 36 hours of ischemic stroke onset.

Design, Setting, and Participants

The Treatment Evaluation of Acute Stroke Using Regenerative Cells (TREASURE) multicenter, double-blind, parallel-group, placebo-controlled phase 2/3 randomized clinical trial was conducted at 44 academic and clinical centers in Japan between November 15, 2017, and March 29, 2022. Inclusion criteria were age 20 years or older, presence of acute ischemic stroke (National Institutes of Health Stroke Scale [NIHSS] score of 8-20 at baseline), confirmed acute infarction involving the cerebral cortex and measuring more than 2 cm on the major axis (determined with diffusion-weighted magnetic resonance imaging), and a modified Rankin Scale (mRS) score of 0 or 1 before stroke onset. Data analysis was performed between May 9 and August 15, 2022.

Exposure

Patients were randomly assigned to either intravenous MultiStem in 1 single unit of 1.2 billion cells or intravenous placebo within 18 to 36 hours of ischemic stroke onset.

Main Outcomes and Measures

The primary end points were safety and excellent outcome at day 90, measured as a composite of a modified Rankin Scale (mRS) score of 1 or less, a NIHSS score of 1 or less, and a Barthel index score of 95 or greater. The secondary end points were excellent outcome at day 365, mRS score distribution at days 90 and 365, and mRS score of 0 to 1 and 0 to 2 at day 90. Statistical analysis of efficacy was performed using the Cochran-Mantel-Haenszel test.

Results

This study included 206 patients (104 received MultiStem and 102 received placebo). Their mean age was 76.5 (range, 35-95) years, and more than half of patients were men (112 [54.4%]). There were no between-group differences in primary and secondary end points. The proportion of excellent outcomes at day 90 did not differ significantly between the MultiStem and placebo groups (12 [11.5%] vs 10 [9.8%], P = .90; adjusted risk difference, 0.5% [95% CI, −7.3% to 8.3%]). The frequency of adverse events was similar between treatment groups.

Conclusions and Relevance

In this randomized clinical trial, intravenous administration of allogeneic cell therapy within 18 to 36 hours of ischemic stroke onset was safe but did not improve short-term outcomes. Further research is needed to determine whether MultiStem therapy for ischemic stroke has a beneficial effect in patients who meet specific criteria, as indicated by the exploratory analyses in this study.

Trial Registration

ClinicalTrials.gov Identifier: NCT02961504

A validation study for wide-range remote assessment of cognitive functions in the healthy older Japanese population: a pilot randomised crossover trial.
Yuki Takakura, Mika Otsuki, Ryo Takagi, Kiyohiro Houkin
BMC geriatrics, 23, 1, 575, 575, 19 Sep. 2023, [International Magazine]
English, Scientific journal, BACKGROUND: The assessment of a wide range of cognitive functions using video teleconference (VTC) systems cannot be applied in practice yet. We aimed to determine the feasibility and reliability of previously unvalidated remote cognitive function tests in Japan using common information and communication technology (ICT) devices, software, and VTC systems compared with face-to-face (FTF) assessment. METHODS: The sample consisted of 26 participants from senior citizens clubs and an employment service centre in Sapporo Japan, including 11 females and 15 males (age averaged 78.6 ± 6.8 years). Tests included the RCPM, Story recall, 10/36 spatial recall, selective reminding test, SDMT, PASAT, FAB, TMT-A, TMT-B, visual cancellation task, digit span, tapping span. The experimental design was a counterbalanced crossover randomised controlled trial. Intraclass correlations (ICCs), paired-samples t-tests, Cohen's Kappa (κ) coefficients, and Wilcoxon signed-rank test were calculated to compare the scores between VTC and FTF assessments. RESULTS: All ICCs were significant and ranged from 0.47 (RCPM time) to 0.92 (RCPM score and PASAT), with a mean ICC of 0.75. Digit span using Cohen's Kappa (κ) coefficient was significant, but the tapping span was not. Paired samples t-test showed statistically significant differences in SDMT, RCPM time, and cancellation time. CONCLUSIONS: The results suggest that remote video conference-based neuropsychological tests even using familiar devices and software may be able to assess a wide range of cognitive functions in the Japanese older population. As for the processing speed tasks, we need to create our own standards for the remote condition. For the tapping span, we should consider increasing the number of trials.

Dual Antiplatelet Therapy With Cilostazol for Secondary Prevention in Lacunar Stroke: Subanalysis of the CSPS.com Trial.
Yasuhiro Nishiyama, Kazumi Kimura, Toshiaki Otsuka, Kazunori Toyoda, Shinichiro Uchiyama, Haruhiko Hoshino, Nobuyuki Sakai, Yasushi Okada, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Kazuo Minematsu, Masayasu Matsumoto, Teiji Tominaga, Hidekazu Tomimoto, Yasuo Terayama, Satoshi Yasuda, Takenori Yamaguchi
Stroke, 54, 3, 697, 705, Mar. 2023, [International Magazine]
English, Scientific journal, BACKGROUND: The effectiveness of long-term dual antiplatelet therapy (DAPT) to prevent recurrent strokes in patients with lacunar stroke remains unclarified. Therefore, this study aimed to compare and to elucidate the safety and effectiveness of DAPT and single antiplatelet therapy (SAPT) in preventing recurrence in chronic lacunar stroke. METHODS: CSPS.com (Cilostazol Stroke Prevention Study for Antiplatelet Combination) was a prospective, multicenter, randomized controlled trial. In this prespecified subanalysis, 925 patients (mean age, 69.5 years; 69.4% men) with lacunar stroke were selected from 1884 patients with high-risk noncardioembolic stroke, enrolled in the CSPS.com trial after 8 to 180 days following stroke. Patients were randomly assigned to receive either SAPT or DAPT using cilostazol and were followed for 0.5 to 3.5 years. The primary efficacy outcome was the first recurrence of ischemic stroke. The safety outcomes were severe or life-threatening bleeding. RESULTS: The DAPT group receiving cilostazol and either aspirin or clopidogrel and SAPT group receiving aspirin or clopidogrel alone comprised 464 (50.2%) and 461 (49.8%) patients, respectively. Ischemic stroke occurred in 12 of 464 patients (1.84 per 100 patient-years) in the DAPT group and 31 of 461 patients (4.42 per 100 patient-years) in the SAPT group, during follow-up. After adjusting for multiple potential confounding factors, ischemic stroke risk was significantly lower in the DAPT group than in the SAPT group (hazard ratio, 0.43 [95% CI, 0.22-0.84]). The rate of severe or life-threatening hemorrhage did not differ significantly between the groups (2 patients [0.31 per 100 patient-years] versus 6 patients [0.86 per 100 patient-years] in the DAPT and SAPT groups, respectively; hazard ratio, 0.36 [95% CI, 0.07-1.81]). CONCLUSIONS: In patients with lacunar stroke, DAPT using cilostazol had significant benefits in reducing recurrent ischemic stroke incidence compared with SAPT without increasing the risk of severe or life-threatening bleeding. REGISTRATION: URL: https://www. CLINICALTRIALS: gov; Unique identifier: NCT01995370. URL: https://www.umin.ac.jp/ctr; Unique identifier: UMIN000012180.

Cost-effectiveness of seven-days-per-week rehabilitation schedule for acute stroke patients.
Yasuhiro Morii, Kagari Abiko, Toshiya Osanai, Jiro Takami, Takumi Tanikawa, Kensuke Fujiwara, Kiyohiro Houkin, Katsuhiko Ogasawara
Cost effectiveness and resource allocation : C/E, 21, 1, 12, 12, 01 Feb. 2023, [International Magazine]
English, Scientific journal, BACKGROUND: Rehabilitation is an essential medical service for patients who have suffered acute stroke. Although the effectiveness of 7-days-per-week rehabilitation schedule has been studied in comparison with 5- or 6-days-per-week rehabilitation schedule, its cost-effectiveness has not been analyzed. In this research, to help formulate more cost-effective medical treatments for acute stroke patients, we analyzed the cost-effectiveness of 7-days-per-week rehabilitation for acute stroke from public health payer's perspective, and public healthcare and long-term care payer's perspective in Japan. METHODS: Cost-effectiveness of 7-days-per-week rehabilitation for acute stroke patients was analyzed based on the result from a previous study using a Japanese database examining the efficacy of 7-days-per-week rehabilitation. Cost utility analysis was conducted by comparing 7-days-per-week rehabilitation with 5- or 6-days-per-week rehabilitation, with its main outcome incremental cost-effectiveness ratio (ICER) calculated by dividing estimated incremental medical and long-term care costs by incremental quality-adjusted life years (QALY). The costs were estimated using the Japanese fee table and from published sources. The time horizon was 5 years, and Markov modeling was used for the analysis. RESULTS: The ICER was $6339/QALY from public health payer's perspective, lower than 5,000,000 Yen/QALY (approximately US$37,913), which was the willingness-to-pay used for the cost-effectiveness evaluation in Japan. The 7-day-per-week rehabilitation was dominant from public healthcare and long-term care payer's perspective. The result of sensitivity analysis confirmed the results. CONCLUSION: The results indicated that 7-days-per-week rehabilitation for acute stroke rehabilitation was likely to be cost-effective.

高齢者における脳トレアプリの効果的な使用条件の検討 PROBE法での無作為化比較試験
高倉 祐樹, 大槻 美佳, 高木 諒, 寳金 清博
老年精神医学雑誌, 33, 増刊II, 356, 356, (株)ワールドプランニング, Nov. 2022
Japanese

高齢者における脳トレアプリの効果的な使用条件の検討 PROBE法での無作為化比較試験　　　　　　　　　　　　　　　
高倉 祐樹, 大槻 美佳, 高木 諒, 寳金 清博
Dementia Japan, 36, 4, 802, 802, (一社)日本認知症学会, Oct. 2022
Japanese

Impact of RNF213 c.14576G>A Variant on the Development of Direct and Indirect Revascularization in Pediatric Moyamoya Disease
Masahito Kawabori, Masaki Ito, Ken Kazumata, Kikutaro Tokairin, Kanako C. Hatanaka, Susumu Ishikawa, Kiyohiro Houkin, Miki Fujimura
Cerebrovascular Diseases, 52, 2, 171, 176, S. Karger AG, 05 Sep. 2022
Scientific journal, Background: Superficial temporal artery (direct) and encephalomyosynangiosis (indirect) revascularization may develop after combined bypass surgery in pediatric patients with moyamoya disease (MMD). However, arterial development varies widely among patients, and the underlying mechanisms remain unknown. Objectives: We evaluated the relationship between the development of donor arteries after bypass surgery in pediatric patients with MMD and the MMD-susceptibility gene variant c.14576G>A of ring finger protein (RNF) 213. Methods: The data of pediatric patients with MMD (age <16 years at the time of surgery) treated with combined bypass surgery between September 2013 and April 2019 were consecutively analyzed. Quantitative measurements of the superficial temporal artery (STA), deep temporal artery (DTA), and middle meningeal artery (MMA) diameters with magnetic resonance angiography (MRA) source imaging were performed preoperatively and at 6–12 months postoperatively. The postoperative caliber change ratios (CCRs) were calculated. The relationship between CCRs and RNF213 c.14576G>A status was examined. Results: Forty-eight hemispheres from 28 pediatric patients with MMD were examined. Three hemispheres belonged to patients with the AA genotype; 33 to patients with the AG genotype (AA/AG group); and 12 to patients with the GG genotype (GG group; wild type). The CCRs for the DTA were significantly higher in patients with RNF213 variant (AA/AG group; 2.5 ± 0.1) than in the GG group (2.0 ± 0.2) (p = 0.03), whereas the CCRs for the STA were significantly higher in the GG (1.6 ± 0.1) than in the AA/AG group (1.3 ± 0.6) (p = 0.02). There was no significant difference in the CCRs for the MMA and basilar artery between the groups. Other factors, including sex, age, and MRA grading, were not associated with the development of specific bypass development. Conclusions: The extent of collateral development associated with direct or indirect bypass was found to differ between the genotypes of the RNF213 c.14576G>A associated with pediatric MMD. This genetic variant correlates with the development of the disease and affects revascularization after bypass surgery in pediatric patients with MMD.

脳深部刺激療法術後4年の経過に関する12例の検討　　　　　　　　　　　　　　　
白井 慎一, 江口 克紀, 山崎 和義, 松島 理明, 加納 崇裕, 笹森 徹, 平田 健司, 大槻 美佳, 北川 まゆみ, 寳金 清博, 佐々木 秀直, 藤村 幹, 矢部 一郎
パーキンソン病・運動障害疾患コングレスプログラム・抄録集, 16回, 80, 80, Movement Disorder Society of Japan (MDSJ), Jul. 2022
Japanese

脳深部刺激療法術後4年の経過に関する12例の検討　　　　　　　　　　　　　　　
白井 慎一, 江口 克紀, 山崎 和義, 松島 理明, 加納 崇裕, 笹森 徹, 平田 健司, 大槻 美佳, 北川 まゆみ, 寳金 清博, 佐々木 秀直, 藤村 幹, 矢部 一郎
パーキンソン病・運動障害疾患コングレスプログラム・抄録集, 16回, 80, 80, Movement Disorder Society of Japan (MDSJ), Jul. 2022
Japanese

Analysis of induced pluripotent stem cell clones derived from a patient with mosaic neurofibromatosis type 2.
Yukitomo Ishi, Takumi Era, Sayaka Yuzawa, Michinari Okamoto, Ryosuke Sawaya, Hiroaki Motegi, Shigeru Yamaguchi, Shunsuke Terasaka, Kiyohiro Houkin, Miki Fujimura
American journal of medical genetics. Part A, 18 Feb. 2022, [International Magazine]
English, The diagnosis of mosaicism is challenging in patients with neurofibromatosis type 2 (NF2) subset due to low variant allele frequency. In this study, we generated induced pluripotent stem cells (iPSCs) were generated from a patient clinically diagnosed with NF2 based on multiple schwannomas, including bilateral vestibular schwannomas and meningiomas. Genetic analysis of the patient's mononuclear cells (MNCs) from peripheral blood failed to detect NF2 alteration but successfully found p.Q65X (c.193C>T) mutation in all separate tumors with three intracranial meningiomas and one intraorbital schwannoma, and confirming mosaicism diagnosis in NF2 alteration using deep sequencing. Five different clones with patient-derived iPSCs were established from MNCs in peripheral blood, which showed sufficient expression of pluripotent markers. Genetic analysis showed that one of five generated iPSC lines from MNCs had the same p.Q65X mutation as that found in NF2. There was no significant difference in the expression of genes related to NF2 between iPSC clones with the wild-type and mutant NF2. In this case, clonal expansion of mononuclear bone marrow-derived stem cells recapitulated mosaicism's genetic alteration in NF2. Patient-derived iPSCs from mosaic NF2 would contribute to further functional research of NF2 alteration.

Impact of RNF213 founder polymorphism (p.R4810K) on the postoperative development of indirect pial synangiosis after direct/indirect combined revascularization surgery for adult Moyamoya disease.
Masaki Ito, Masahito Kawabori, Taku Sugiyama, Kikutaro Tokairin, Ryota Tatezawa, Haruto Uchino, Ken Kazumata, Kiyohiro Houkin, Miki Fujimura
Neurosurgical review, 45, 3, 2305, 2313, 07 Feb. 2022, [International Magazine]
English, Scientific journal, Direct superficial temporal artery (STA) to middle cerebral artery (MCA) anastomosis combined with indirect pial synangiosis provides favorable surgical collaterals for Moyamoya disease (MMD), especially in adults; however, factors leading to the development of each direct and indirect collateral are not well documented.We aimed to investigate the association between RNF213 founder polymorphism (p.R4810K) and each direct and indirect collateral development. By qualitative and quantitative evaluations of direct and indirect surgical collaterals using time-of-flight MR angiography, postoperative development of each type of bypass was evaluated independently into two categories. Multivariate logistic regression analysis was performed to study the contributing factors for the development of each surgical collateral. Excellent development of postoperative direct and indirect bypass was observed in 65 hemispheres (70%) by qualitative evaluation, which was confirmed by quantitative evaluation. Multivariate logistic regression analysis of excellent indirect bypass development revealed a significant positive correlation with the p.R4810K (odds ratio, OR4.0; 95%-confidence interval, CI 1.2-16), advanced MR angiographic stage (OR9.5; 95%CI 1.7-73), and preoperative middle meningeal artery caliber (OR6.8; 95%CI 1.8-35), but a significant negative correlation was found with the excellent direct bypass development (OR0.17; 95%CI 0.03-0.75). No significant correlation was observed between excellent direct bypass development and the p.R4810K (OR0.95; 95%CI 0.37-2.4).In conclusion, excellent development of indirect collaterals after STA-MCA anastomosis combined with indirect pial synangiosis occurs more frequently in adult MMD with the RNF213 founder polymorphism, suggesting a role of the p.R4810K variant for marked in-growth of indirect collaterals and the utility of preoperative genetic analysis.

Cystic Intracranial Recurrence of Olfactory Neuroblastoma without Accumulation on Fluorine-18-fluorodeoxyglucose Positron Emission Tomography.
Yukitomo Ishi, Shigeru Yamaguchi, Kanako C Hatanaka, Emi Takakuwa, Hiroaki Motegi, Taishi Honda, Hiroyuki Kobayashi, Shunsuke Terasaka, Akihiro Homma, Miki Fujimura, Kiyohiro Houkin
Acta medica Okayama, 76, 1, 93, 98, Feb. 2022, [Domestic magazines]
English, A 66-year-old man underwent multimodal treatment for olfactory neuroblastoma (ONB). When he was 72 years old, a cystic intracranial lesion without accumulation on fluorine-18-fluorodeoxyglucose positron emission tomography was detected. Surgical resection was performed when the patient was 73 years old. The pathological examination revealed recurrence of ONB, and the patient underwent focal irradiation. At age 81, he presented with a second recurrence in the right occipital lobe with radiological and pathological findings similar to the prior recurrence. This case suggests that pathological confirmation should be considered in cases with atypical radiological findings following the treatment of ONB.

Intravenous transplantation of amnion-derived mesenchymal stem cells promotes functional recovery and alleviates intestinal dysfunction after spinal cord injury.
Soichiro Takamiya, Masahito Kawabori, Kazuyoshi Yamazaki, Sho Yamaguchi, Aki Tanimori, Koji Yamamoto, Shunsuke Ohnishi, Toshitaka Seki, Kotaro Konno, Khin Khin Tha, Daigo Hashimoto, Masahiko Watanabe, Kiyohiro Houkin, Miki Fujimura
PloS one, 17, 7, e0270606, 2022, [International Magazine]
English, Scientific journal, Spinal cord injury (SCI) is often accompanied by gastrointestinal dysfunction due to the disconnection of the spinal autonomic nervous system. Gastrointestinal dysfunction reportedly upregulates intestinal permeability, leading to bacterial translocation of the gut microbiome to the systemic circulation, which further activates systemic inflammation, exacerbating neuronal damage. Mesenchymal stem cells (MSC) reportedly ameliorate SCI. Here, we aimed to investigate their effect on the associated gastrointestinal dysfunction. Human amnion-derived MSC (AMSCs) were intravenously transplanted one day after a rat model of midthoracic SCI. Biodistribution of transplanted cells, behavioral assessment, and histological evaluations of the spinal cord and intestine were conducted to elucidate the therapeutic effect of AMSCs. Bacterial translocation of the gut microbiome was examined by in situ hybridization and bacterial culture of the liver. Systemic inflammations were examined by blood cytokines, infiltrating immune cells in the spinal cord, and the size of the peripheral immune tissue. AMSCs released various neurotrophic factors and were mainly distributed in the liver and lung after transplantation. AMSC-transplanted animals showed smaller spinal damage and better neurological recovery with preserved neuronal tract. AMSCs transplantation ameliorated intestinal dysfunction both morphologically and functionally, which prevented translocation of the gut microbiome to the systemic circulation. Systemic inflammations were decreased in animals receiving AMSCs in the chronic phase. Intravenous AMSC administration during the acute phase of SCI rescues both spinal damage and intestinal dysfunction. Reducing bacterial translocation may contribute to decreasing systemic inflammation.

The prognostic improvement of add-on bevacizumab for progressive disease during concomitant temozolomide and radiation therapy in patients with glioblastoma and anaplastic astrocytoma
Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Michinari Okamoto, Hiroyuki Kobayashi, Kenji Hirata, Yoshitaka Oda, Shinya Tanaka, Shunsuke Terasaka, Kiyohiro Houkin
Journal of Neurosurgical Sciences, 64, 6, 502, 508, Edizioni Minerva Medica, 01 Dec. 2021
English, Scientific journal

脳血管障害の病態評価における脳画像の有用性 もやもや病の周術期画像マーカーとRNF213遺伝子多型の関連　　　　　　　　　　　　　　　
内野 晴登, 伊東 雅樹, 東海林 菊太郎, 舘澤 諒大, 川堀 真人, 数又 研, 宝金 清博, 藤村 幹
脳循環代謝, 33, 1, 70, 70, (一社)日本脳循環代謝学会, Nov. 2021
Japanese

RNF213創始者変異は成人もやもや病複合血行再建術後の間接バイパス路発達に寄与する　　　　　　　　　　　　　　　
伊東 雅基, 川堀 真人, 杉山 拓, 東海林 菊太郎, 舘澤 諒大, 内野 晴登, 数又 研, 寳金 清博, 藤村 幹
脳循環代謝, 33, 1, 94, 94, (一社)日本脳循環代謝学会, Nov. 2021
Japanese

RNF213創始者変異は成人もやもや病複合血行再建術後の間接バイパス路発達に寄与する　　　　　　　　　　　　　　　
伊東 雅基, 川堀 真人, 杉山 拓, 東海林 菊太郎, 舘澤 諒大, 内野 晴登, 数又 研, 寳金 清博, 藤村 幹
脳循環代謝, 33, 1, 94, 94, (一社)日本脳循環代謝学会, Nov. 2021
Japanese

Dual Antiplatelet Therapy Using Cilostazol in Patients With Stroke and Intracranial Arterial Stenosis.
Shinichiro Uchiyama, Kazunori Toyoda, Katsuhiro Omae, Ryotaro Saita, Kazumi Kimura, Haruhiko Hoshino, Nobuyuki Sakai, Yasushi Okada, Kortaro Tanaka, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Masanori Isobe, Kazuo Minematsu, Masayasu Matsumoto, Teiji Tominaga, Hidekazu Tomimoto, Yasuo Terayama, Satoshi Yasuda, Takenori Yamaguchi
Journal of the American Heart Association, 10, 20, e022575, 19 Oct. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Background Long-term benefit of dual antiplatelet therapy (DAPT) over single antiplatelet therapy (SAPT) for the prevention of recurrent stroke has not been established in patients with intracranial arterial stenosis. We compared the efficacy and safety of DAPT with cilostazol and clopidogrel or aspirin to those of SAPT with clopidogrel or aspirin in patients with intracranial arterial stenosis, who were recruited to the Cilostazol Stroke Prevention Study for Antiplatelet Combination trial, a randomized controlled trial in high-risk Japanese patients with ischemic stroke. Methods and Results We compared the vascular and hemorrhagic events between DAPT and SAPT in patients with ischemic stroke and symptomatic or asymptomatic intracranial arterial stenosis of at least 50% in a major intracranial artery. Patients were placed in two groups: 275 were assigned to receive DAPT and 272 patients SAPT. The risks of ischemic stroke (hazard ratio [HR], 0.47; 95% CI, 0.23-0.95); and composite of stroke, myocardial infarction, and vascular death (HR, 0.48; 95% CI, 0.26-0.91) were lower in DAPT than SAPT, whereas the risk of severe or life-threatening bleeding (HR, 0.72; 95% CI, 0.12-4.30) did not differ between the 2 treatment groups. Conclusions DAPT using cilostazol was superior to SAPT with clopidogrel or aspirin for the prevention of recurrent stroke and vascular events without increasing bleeding risk among patients with intracranial arterial stenosis after stroke. Registration URL: https://www.clinicaltrials.gov; Unique identifier: NCT01995370.

妄想対象が「死別した近親者」ではない養生(nurturing)行動の発現機序　　　　　　　　　　　　　　　
高倉 祐樹, 大槻 美佳, 中川 賀嗣, 寳金 清博
Dementia Japan, 35, 4, 635, 635, (一社)日本認知症学会, Oct. 2021
Japanese

Efficacy of Carotid Artery Stenting Performed under General Anesthesia with Somatosensory Evoked Potential Monitoring.
Masayuki Gekka, Toshiya Osanai, Takeshi Aoki, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin, Miki Fujimura
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 30, 10, 106007, 106007, Oct. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

Correlation of active contact location with weight gain after subthalamic nucleus deep brain stimulation: a case series.
Katsuki Eguchi, Shinichi Shirai, Masaaki Matsushima, Takahiro Kano, Kazuyoshi Yamazaki, Shuji Hamauchi, Toru Sasamori, Toshitaka Seki, Kenji Hirata, Mayumi Kitagawa, Mika Otsuki, Tohru Shiga, Kiyohiro Houkin, Hidenao Sasaki, Ichiro Yabe
BMC neurology, 21, 1, 351, 351, 13 Sep. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

Genetic and nongenetic factors for contralateral progression of unilateral moyamoya disease: the first report from the SUPRA Japan Study Group.
Yohei Mineharu, Yasushi Takagi, Akio Koizumi, Takaaki Morimoto, Takeshi Funaki, Tomohito Hishikawa, Yoshio Araki, Hitoshi Hasegawa, Jun C Takahashi, Satoshi Kuroda, Kiyohiro Houkin, Susumu Miyamoto
Journal of neurosurgery, 136, 4, 1, 10, 10 Sep. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: Although many studies have analyzed risk factors for contralateral progression in unilateral moyamoya disease, they have not been fully elucidated. The aim of this study was to examine whether genetic factors as well as nongenetic factors are involved in the contralateral progression. METHODS: The authors performed a multicenter cohort study in which 93 cases with unilateral moyamoya disease were retrospectively reviewed. The demographic features, RNF213 R4810K mutation, lifestyle factors such as smoking and drinking, past medical history, and angiographic findings were analyzed. A Cox proportional hazards model was used to find risk factors for contralateral progression. RESULTS: Contralateral progression was observed in 24.7% of cases during a mean follow-up period of 72.2 months. Clinical characteristics were not significantly different between 63 patients with the R4810K mutation and those without it. Cox regression analysis showed that the R4810K mutation (hazard ratio [HR] 4.64, p = 0.044), childhood onset (HR 7.21, p < 0.001), male sex (HR 2.85, p = 0.023), and daily alcohol drinking (HR 4.25, p = 0.034) were independent risk factors for contralateral progression. CONCLUSIONS: These results indicate that both genetic and nongenetic factors are associated with contralateral progression of unilateral moyamoya disease. The findings would serve to help us better understand the pathophysiology of moyamoya disease and to manage patients more appropriately.

Dual Antiplatelet Therapy Using Cilostazol With Aspirin or Clopidogrel: Subanalysis of the CSPS.com Trial.
Haruhiko Hoshino, Kazunori Toyoda, Katsuhiro Omae, Noriyuki Ishida, Shinichiro Uchiyama, Kazumi Kimura, Nobuyuki Sakai, Yasushi Okada, Kortaro Tanaka, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Masanori Isobe, Kazuo Minematsu, Masayasu Matsumoto, Teiji Tominaga, Hidekazu Tomimoto, Yasuo Terayama, Satoshi Yasuda, Takenori Yamaguchi
Stroke, 52, 11, STROKEAHA121034378, 3439, 18 Aug. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND AND PURPOSE: Although dual antiplatelet therapy (DAPT) with aspirin and clopidogrel reduces the recurrence of ischemic stroke while significantly increasing the bleeding events compared with monotherapy, the CSPS.com trial (Cilostazol Stroke Prevention Study combination) showed that DAPT using cilostazol was more effective without the bleeding risk. In the CSPS.com trial, aspirin or clopidogrel was used as the underlying antiplatelet drug. The effectiveness and safety of each combination were examined and clarified. METHODS: In the CSPS.com trial, a multicenter, open-label, randomized controlled study, patients with high-risk, noncardioembolic ischemic stroke 8 to 180 days after onset treated with aspirin or clopidogrel alone at the discretion of the physician in charge were recruited. Patients were randomly assigned to receive either monotherapy or DAPT using cilostazol and followed for 0.5 to 3.5 years. The primary efficacy outcome was first recurrence of ischemic stroke. The safety outcome was severe or life-threatening bleeding. The analysis was based on the underlying antiplatelet agents. RESULTS: A total of 763 patients taking aspirin and 1116 taking clopidogrel were included in the intention-to-treat analysis. Although the clopidogrel group had more risk factors than the aspirin group, the primary efficacy outcome and safety outcome did not differ significantly between the 2 groups. In the aspirin group, the primary efficacy outcome and safety outcome did not differ significantly between the DAPT group and the aspirin-monotherapy group. In the clopidogrel group, the primary end point occurred at a rate of 2.31 per 100 patient-years in the DAPT group and 5.19 per 100 patient-years in the clopidogrel-monotherapy group (hazard ratio, 0.447 [95% CI, 0.258-0.774]). Safety outcome did not differ significantly between groups (0.51 per 100 patient-years versus 0.71 per 100 patient-years, respectively; hazard ratio, 0.730 [95% CI, 0.206-2.588]). CONCLUSIONS: The combination of cilostazol and clopidogrel significantly reduced the recurrence of ischemic stroke without increasing the bleeding risk in noncardioembolic, high-risk patients. REGISTRATION: URL: http://www.clinicaltrials.gov; Unique identifier: NCT01995370. URL: https://www.umin.ac.jp/ctr/; Unique identifier: UMIN000012180.

Analyzing Cost-Effectiveness of Allocating Neurointerventionist for Drive and Retrieve System for Patients with Acute Ischemic Stroke.
Yasuhiro Morii, Toshiya Osanai, Kensuke Fujiwara, Takumi Tanikawa, Kiyohiro Houkin, Songzi Gu, Katsuhiko Ogasawara
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 30, 8, 105843, 105843, Aug. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

Chronic deep brain stimulation reduces cortical β-γ phase amplitude-coupling in patients with Parkinson's disease.
Katsuki Eguchi, Shinichi Shirai, Masaaki Matsushima, Takahiro Kano, Tomohiro Ichikawa, Kazuyoshi Yamazaki, Shuji Hamauchi, Toru Sasamori, Toshitaka Seki, Mayumi Kitagawa, Hideaki Shiraishi, Kiyohiro Houkin, Hidenao Sasaki, Ichiro Yabe
Parkinsonism & related disorders, 89, 148, 150, Aug. 2021, [Peer-reviewed], [International Magazine]
English

Clinical Outcome of Cytoreductive Surgery Prior to Bevacizumab for Patients with Recurrent Glioblastoma: A Single-center Retrospective Analysis.
Shigeru Yamaguchi, Hiroaki Motegi, Yukitomo Ishi, Michinari Okamoto, Ryosuke Sawaya, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
Neurologia medico-chirurgica, 61, 4, 245, 252, 15 Apr. 2021, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Intracerebral Transplantation of Autologous Bone Marrow Stem Cell (BMSC) for Subacute Ischemic Stroke, Phase 1 Clinical Trial (RAINBOW Trial)
Masahito Kawabori, Hideo Shichinohe, Satoshi Kuroda, Kiyohiro Houkin
STROKE, 52, Mar. 2021, [Peer-reviewed]
English

Novel risk factors and management of brain sag after brain tumor surgery
Hiroaki Motegi, Shigeru Yamaguchi, Michinari Okamoto, Yukitomo Ishi, Shogo Endo, Sadahiro Kaneko, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
INTERDISCIPLINARY NEUROSURGERY-ADVANCED TECHNIQUES AND CASE MANAGEMENT, 23, Mar. 2021, [Peer-reviewed]
English, Scientific journal

Immersive 3-Dimensional Virtual Reality Modeling for Case-Specific Presurgical Discussions in Cerebrovascular Neurosurgery.
Taku Sugiyama, Tod Clapp, Jordan Nelson, Chad Eitel, Hiroaki Motegi, Naoki Nakayama, Tsukasa Sasaki, Kikutaro Tokairin, Masaki Ito, Ken Kazumata, Kiyohiro Houkin
Operative neurosurgery (Hagerstown, Md.), 20, 3, 289, 299, 16 Feb. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

Vulnerability to shear stress caused by altered peri-endothelial matrix is a key feature of Moyamoya disease.
Muneaki Matsuo, Satomi Nadanaka, Minami Soga, Taku Sugiyama, Shota Serigano, Kenjiro Shimano, Fumio Ichinose, Takuji Nakamura, Toshiyuki Maeda, Kiyohiro Houkin, Takumi Era, Hiroshi Kitagawa
Scientific reports, 11, 1, 1552, 1552, 15 Jan. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

慢性期頭部外傷患者に対する間葉系幹細胞(SB623)脳内投与の治験成績(STEMTRA研究)　　　　　　　　　　　　　　　
川堀 真人, 今井 英明, 安原 隆雄, 末永 潤, 中村 元, 唐沢 康暉, 宝金 清博
日本脳神経外傷学会プログラム・抄録集, 44回, 59, 59, (一社)日本脳神経外傷学会, Jan. 2021
Japanese

The Japan Neurosurgical Database: Statistics Update 2018 and 2019
Koji IIHARA, Nobuhito SAITO, Michiyasu SUZUKI, Isao DATE, Yukihiko FUJII, Kiyohiro HOUKIN, Tooru INOUE, Toru IWAMA, Takakazu KAWAMATA, Phyo KIM, Hiroyuki KINOUCHI, Haruhiko KISHIMA, Eiji KOHMURA, Kaoru KURISU, Keisuke MARUYAMA, Yuji MATSUMARU, Nobuhiro MIKUNI, Susumu MIYAMOTO, Akio MORITA, Hiroyuki NAKASE, Yoshitaka NARITA, Ryo NISHIKAWA, Kazuhiko NOZAKI, Kuniaki OGASAWARA, Kenji OHATA, Nobuyuki SAKAI, Hiroaki SAKAMOTO, Yoshiaki SHIOKAWA, Jun C. TAKAHASHI, Keisuke UEKI, Toshihiko WAKABAYASHI, Koji YOSHIMOTO, Hajime ARAI, Teiji TOMINAGA
Neurologia medico-chirurgica, Japan Neurosurgical Society, 2021
Scientific journal

Corrigendum to "Evaluation of Novel Stereotactic Cannula for Stem Cell Transplantation against Central Nervous System Disease".
Masahito Kawabori, Aki Tanimori, Shinri Kitta, Hideo Shichinohe, Kiyohiro Houkin
Stem cells international, 2021, 9796010, 9796010, 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

Mesenchymal Stem Cell Sheet Promotes Functional Recovery and Palliates Neuropathic Pain in a Subacute Spinal Cord Injury Model.
Kazuyoshi Yamazaki, Masahito Kawabori, Toshitaka Seki, Soichiro Takamiya, Kotaro Konno, Masahiko Watanabe, Kiyohiro Houkin, Miki Fujimura
Stem cells international, 2021, 9964877, 9964877, 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

CAN HIGH LEVEL SERUM HCG-B BE CONSIDERED EQUIVALENT TO A DIAGNOSIS OF CHORIOCARCINOMA IN PRIMARY CENTRAL NERVOUS SYSTEM GERM-CELL TUMOR?
Hiroaki Motegi, Shigeru Yamaguchi, Yukitomo Ishi, Michinari Okamoto, Akihiro Iguchi, Yuko Cho, Minako Sugiyama, Atsushi Manabe, Kiyohiro Houkin
NEURO-ONCOLOGY, 22, 329, 330, Dec. 2020, [Peer-reviewed]
English

DIAGNOSTIC CAPABILITY OF CSF-PLAP ON INTRACRANIAL GERM CELL TUMOR
Michinari Okamoto, Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Yukayo Terashita, Shinsuke Hirabayashi, Minako Sugiyama, Yuko Cho, Akihiro Iguchi, Atushi Manabe, Kiyohiro Houkin
NEURO-ONCOLOGY, 22, 334, 334, Dec. 2020, [Peer-reviewed]
English

INITIAL MANAGEMENT OF HYDROCEPHALUS IN THE PEDIATRIC AND YOUNG-ADULT PATIENTS WITH BRAIN TUMORS; THE EFFICACY OF LONG-TERM INDWELLING EXTERNAL VENTRICULAR DRAINAGE
Shigeru Yamaguchi, Hiroaki Motegi, Yukitomo Ishi, Michinari Okamoto, Akihiro Iguchi, Yuko Cho, Minako Sugiyama, Atushi Manabe, Kiyohiro Houkin
NEURO-ONCOLOGY, 22, 461, 461, Dec. 2020, [Peer-reviewed]
English

脳梗塞に対する新規治療の開発-血栓回収療法時代のアンメットメディカルニーズと医薬品開発戦略- 主幹動脈閉塞脳梗塞患者に対する幹細胞脳内移植治験(RAINBOW研究)　　　　　　　　　　　　　　　
川堀 真人, 七戸 秀夫, 黒田 敏, 宝金 清博
脳循環代謝, 32, 1, 52, 52, (一社)日本脳循環代謝学会, Nov. 2020
Japanese

脳梗塞急性期患者への自家BMSC脳内投与による再生治療(Phase 1)とPhase 2に向けた取り組み　　　　　　　　　　　　　　　
川堀 真人, 七戸 秀夫, 黒田 敏, 宝金 清博
脳循環代謝, 32, 1, 60, 60, (一社)日本脳循環代謝学会, Nov. 2020
Japanese

脳神経細胞治療・遺伝子治療の実践 脳出血慢性期モデルに対する骨髄幹細胞+足場材製剤(CellSaic)の有効性　　　　　　　　　　　　　　　
高宮 宗一朗, 川堀 真人, 七戸 秀夫, 中村 健太郎, 北橋 宗, 岩澤 玲子, 古川 友子, 宝金 清博
脳循環代謝, 32, 1, 68, 68, (一社)日本脳循環代謝学会, Nov. 2020
Japanese

ラット亜急性期脊髄損傷モデルに対する骨髄間葉系幹細胞シート移植の治療効果検証　　　　　　　　　　　　　　　
山崎 和義, 川堀 真人, 関 俊隆, 高宮 宗一朗, 今野 幸太郎, 渡辺 雅彦, 宝金 清博
脳循環代謝, 32, 1, 82, 82, (一社)日本脳循環代謝学会, Nov. 2020
Japanese

Neuroprotective effects of combination therapy of regional cold perfusion and hemoglobin-based oxygen carrier administration on rat transient cerebral ischemia.
Yasuhiro Ito, Takeo Abumiya, Teruyuki Komatsu, Ryosuke Funaki, Masayuki Gekka, Kota Kurisu, Taku Sugiyama, Masahito Kawabori, Toshiya Osanai, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
Brain research, 1746, 147012, 147012, 01 Nov. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Clinical Trials of Stem Cell Therapy for Cerebral Ischemic Stroke.
Masahito Kawabori, Hideo Shichinohe, Satoshi Kuroda, Kiyohiro Houkin
International journal of molecular sciences, 21, 19, 1, 21, 06 Oct. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

FTY720 Protects Against Ischemia-Reperfusion Injury by Preventing the Redistribution of Tight Junction Proteins and Decreases Inflammation in the Subacute Phase in an Experimental Stroke Model.
Zifeng Wang, Kei Higashikawa, Hironobu Yasui, Yuji Kuge, Yusuke Ohno, Akio Kihara, Yenari A Midori, Kiyohiro Houkin, Masahito Kawabori
Translational stroke research, 11, 5, 1103, 1116, Oct. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Vascular Smooth Muscle Cell Derived from IPS Cell of Moyamoya Disease - Comparative Characterization with Endothelial Cell Transcriptome.
Kikutaro Tokairin, Shuji Hamauchi, Masaki Ito, Ken Kazumata, Taku Sugiyama, Naoki Nakayama, Masahito Kawabori, Toshiya Osanai, Kiyohiro Houkin
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 29, 12, 105305, 105305, 23 Sep. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Specific clinical features and one-stage revascularization surgery for moyamoya disease with severe cerebral ischemia in the territory of posterior cerebral artery.
Hisayasu Saito, Daina Kashiwazaki, Haruto Uchino, Shusuke Yamamoto, Kiyohiro Houkin, Satoshi Kuroda
Acta neurochirurgica, 163, 2, 583, 592, 14 Sep. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Diagnostic Capability of Cerebrospinal Fluid-Placental Alkaline Phosphatase Value in Intracranial Germ Cell Tumor.
Michinari Okamoto, Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Takashi Mori, Takayuki Hashimoto, Yukayo Terashita, Shinsuke Hirabayashi, Minako Sugiyama, Akihiro Iguchi, Yuko Cho, Atsushi Manabe, Kiyohiro Houkin
Oncology, 99, 1, 1, 9, 09 Sep. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Responses of immune organs following cerebral ischemic stroke.
Chengbo Tan, Zifeng Wang, Miao Zheng, Songji Zhao, Hideo Shichinohe, Kiyohiro Houkin
Journal of Nippon Medical School = Nippon Ika Daigaku zasshi, 88, 3, 228, 237, 31 Aug. 2020, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

FTY720 Attenuates Neuropathic Pain after Spinal Cord Injury by Decreasing Systemic and Local Inflammation in a Rat Spinal Cord Compression Model.
Kazuyoshi Yamazaki, Masahito Kawabori, Toshitaka Seki, Soichiro Takamiya, Takahiro Tateno, Kotaro Konno, Masahiko Watanabe, Kiyohiro Houkin
Journal of neurotrauma, 37, 15, 1720, 1728, 01 Aug. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Microsurgical Findings of Pial Arterial Feeders in Intracranial Dural Arteriovenous Fistulae: A Case Series.
Michinari Okamoto, Taku Sugiyama, Naoki Nakayama, Satoshi Ushikoshi, Ken Kazumata, Toshiya Osanai, Kikutaro Tokairin, Yusuke Shimoda, Kiyohiro Houkin
Operative neurosurgery (Hagerstown, Md.), 19, 6, 691, 700, 27 Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Clinical Trials of Stem Cell Treatment for Spinal Cord Injury.
Kazuyoshi Yamazaki, Masahito Kawabori, Toshitaka Seki, Kiyohiro Houkin
International journal of molecular sciences, 21, 11, 02 Jun. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

The Japan Neurosurgical Database: Overview and Results of the First-year Survey.
Koji Iihara, Teiji Tominaga, Nobuhito Saito, Michiyasu Suzuki, Isao Date, Yukihiko Fujii, Kazuhiro Hongo, Kiyohiro Houkin, Amami Kato, Yoko Kato, Takakazu Kawamata, Phyo Kim, Hiroyuki Kinouchi, Eiji Kohmura, Kaoru Kurisu, Keisuke Maruyama, Nobuhiro Mikuni, Susumu Miyamoto, Akio Morita, Hiroyuki Nakase, Yoshitaka Narita, Ryo Nishikawa, Kazuhiko Nozaki, Kuniaki Ogasawara, Kenji Ohata, Nobuyuki Sakai, Hiroaki Sakamoto, Yoshiaki Shiokawa, Yukihiko Sonoda, Jun C Takahashi, Keisuke Ueki, Toshihiko Wakabayashi, Takamitsu Yamamoto, Kazunari Yoshida, Takamasa Kayama, Hajime Arai
Neurologia medico-chirurgica, 60, 4, 165, 190, 15 Apr. 2020, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The Japan Neurosurgical Database (JND) is a prospective observational study registry established in 2017 by the Japan Neurosurgical Society (JNS) to visualize real-world clinical practice, promote science, and improve the quality of care and neurosurgery board certification in Japan. We summarize JND's aims and methods, and describes the 2018 survey results. The JND registered in-hospital patients' clinical data mainly from JNS training institutions in 2018. Caseload, patient demographics, and in-hospital outcomes of the overall cohort and a neurosurgical subgroup were examined according to major classifications of main diagnosis. Neurosurgical caseload per neurosurgeon in training in core hospitals in 2018 was calculated as an indicator of neurosurgical training. Of 523,283 cases (male 55.3%) registered from 1360 participating institutions, the neurosurgical subgroup comprised of 33.9%. Among the major classifications, cerebrovascular diseases comprised the largest proportion overall and in the neurosurgical subgroup (53.1%, 41.0%, respectively), followed by neurotrauma (19.1%, 25.5%), and brain tumor (10.4%, 12.8%). Functional neurosurgery (6.4%, 3.7%), spinal and peripheral nerve disorders (5.1%, 10.1%), hydrocephalus/developmental anomalies (2.9%, 5.3%), and encephalitis/infection/inflammatory and miscellaneous diseases (2.9%, 1.6%) comprised smaller proportions. Most patients were aged 70-79 years in the overall cohort and neurosurgical subgroup (27.8%, 29.4%). Neurotrauma and cerebrovascular diseases in the neurosurgical subgroup comprised a higher and lower proportion, respectively, than in the overall cohort in elderly patients (e.g. 80 years, 46.9% vs. 33.5%, 26.8% vs. 54.4%). The 2018 median neurosurgical caseload per neurosurgeon in training was 80.7 (25-75th percentile 51.5-117.5). These initial results from 2018 reveal unique aspects of neurosurgical practice in Japan.

Combined structural and diffusion tensor imaging detection of ischemic injury in moyamoya disease: relation to disease advancement and cerebral hypoperfusion.
Ken Kazumata, Kikutaro Tokairin, Masaki Ito, Haruto Uchino, Taku Sugiyama, Masahito Kawabori, Toshiya Osanai, Khin Khin Tha, Kiyohiro Houkin
Journal of neurosurgery, 134, 4, 1, 10, 03 Apr. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

[Embolization of Arteriovenous Fistulae of the Maxillary Artery after Le Fort I Osteotomy:A Case Report].
Shuho Gotoh, Toshiya Osanai, Satoshi Ushikoshi, Kota Kurisu, Yusuke Shimoda, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 48, 4, 335, 340, Apr. 2020, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, BACKGROUND: Le Fort I osteotomy, one of the most common techniques applied to correct jaw deformities, is generally considered operatively safe. However, a few articles reported that this procedure can lead to formation of arteriovenous fistulae(AVF)involving the maxillary artery infrequently. CASE REPORT: A man in his 40s with a jaw deformity underwent Le Fort I osteotomy. Two days later, he noticed pulsating tinnitus in his right ear. Angiography revealed AVF between the proximal part of the maxillary artery(first segment)and the pterygoid plexus. On further evaluation, AVF were embolized with n-butyl-2-cyanoacrylate(NBCA). Obliteration of AVF was confirmed on the final angiography. The tinnitus resolved shortly after the embolization. CONCLUSION: Embolization with NBCA is a promising treatment for maxillary AVF after Le Fort I osteotomy.

Predictive factors for acute thrombogenesis occurring immediately after bypass procedure for moyamoya disease.
Takeshi Mikami, Hime Suzuki, Ryo Ukai, Katsuya Komatsu, Yukinori Akiyama, Masahiko Wanibuchi, Kiyohiro Houkin, Nobuhiro Mikuni
Neurosurgical review, 43, 2, 609, 617, Apr. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Extracranial-to-intracranial (EC-IC) bypass surgery is an effective treatment for patients with moyamoya disease and other conditions. Some patients with moyamoya disease have a risk of acute thrombogenesis at the anastomotic site just after bypass surgery. The purpose of this study was to study risk factors of acute thrombogenesis and determine effective countermeasures. This study included 48 patients (66 EC-IC bypass procedures) with moyamoya disease and 52 controls (54 procedures) without moyamoya disease. The development of acute thrombogenesis was compared between the moyamoya disease and control groups. In the moyamoya disease group, clinical and radiological characteristics were assessed with respect to acute thrombogenesis. In the patients with acute thrombogenesis, causes of technical problems were retrospectively examined. The incidence of acute thrombogenesis was significantly higher in the moyamoya disease group than those in the control group. In the moyamoya disease group, acute thrombogenesis was observed in seven patients. In the moyamoya disease group, the magnetic resonance angiography (MRA) scores were significantly higher in patients with acute thrombogenesis than those in the patients without acute thrombogenesis. In the multivariate analysis, the predictive factor of acute thrombogenesis in moyamoya disease was a high MRA score (odds ratio, 2.336; p = 0.009). During EC-IC bypass surgery for moyamoya disease, acute thrombogenesis should be considered to obtain a high patency rate, particularly in patients with high MRA scores. Acute thrombogenesis will not influence morbidity if proper countermeasures are followed; therefore, the prediction and recognition of white thrombus are important for a successful bypass surgery.

A register-based SAH study in Japan: high incidence rate and recent decline trend based on lifestyle.
Fusao Ikawa, Akio Morita, Takeo Nakayama, Yoshihito Goto, Nobuyuki Sakai, Koji Iihara, Yoshiaki Shiokawa, Isao Date, Kazuhiko Nozaki, Hiroyuki Kinouchi, Kiyohiro Houkin, Nobuhito Saito, Teiji Tominaga, Michiyasu Suzuki, Susumu Miyamoto, Kaoru Kurisu, Hajime Arai
Journal of neurosurgery, 134, 3, 1, 9, 27 Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: Japan has been reported to have the highest (and increasing) incidence of subarachnoid hemorrhage (SAH) in the world. However, there has never been a report on the nationwide incidence rate and recent trends for SAH in Japan. In this register-based study, the authors aimed to clarify the estimated nationwide SAH incidence rate and the recent trend in SAH incidence in Japan and the reasons for any changes in this trend. METHODS: The authors compiled data from the Japanese Ministry of Health, Labour and Welfare and from the records of the Japan Neurosurgical Society. They reviewed the age-standardized nationwide SAH mortality rate, the estimated age-standardized SAH incidence rate according to the age-standardized SAH mortality rate, and the estimated crude SAH incidence rate, including the 95% confidence intervals, from 2003 to 2015. The trends in the number of treatments for unruptured and ruptured cerebral aneurysms, as well as the prevalence of hypertension, current smoking status, and use of cholesterol-lowering drugs, were assessed. The estimated treatment rate for unruptured cerebral aneurysms (UCAs) was also calculated along with the 95% confidence interval. RESULTS: The estimated age-standardized nationwide SAH incidence rate significantly declined from 31.34 cases (95% CI 31.32-31.34) to 27.63 (95% CI 27.59-27.63; decrease by 11.8%) per 100,000 person-years. This decline was based on the 2010 population as the reference from 2003 through 2015 and a case-fatality rate of SAH that was assumed to decrease by 1% annually from 44% in 2003 to 32% in 2015. According to sensitivity analysis, the change rate of the estimated age-standardized SAH incidence rate ranged from -56.69% to 23.27%, with a mean of -30.91% (SD 20.52%), and there were decline trends in 92% of all trends. The estimated nationwide crude SAH incidence rate also showed a significant decline from 23.79 (95% CI 23.78-23.79) to 20.25 (95% CI 20.24-20.25; decrease by 14.9%) per 100,000 person-years. The trend in treatment numbers for UCAs increased significantly (p < 0.0001) from 2003 through 2015; however, the estimated treatment rate for UCAs was only 0.19 (95% CI 0.19-0.20) to 0.51 (95% CI 0.50-0.51) among all UCA patients. The prevalence of hypertension (males, p = 0.0003; females, p < 0.0001) and current smoking status (males, p < 0.0001; females, p = 0.0002) declined significantly from 2003 through 2015, while the use of cholesterol-lowering drugs increased significantly (males, p < 0.0001; females, p = 0.0005) during the same period. CONCLUSIONS: The estimated nationwide SAH incidence rate in Japan was higher than rates in other countries, although it has declined recently. An improving lifestyle may have contributed to the declining rate of SAH incidence in Japan.

Late (5-20 years) outcomes after STA-MCA anastomosis and encephalo-duro-myo-arterio-pericranial synangiosis in patients with moyamoya disease.
Satoshi Kuroda, Naoki Nakayama, Shusuke Yamamoto, Daina Kashiwazaki, Haruto Uchino, Hisayasu Saito, Emiko Hori, Naoki Akioka, Naoya Kuwayama, Kiyohiro Houkin
Journal of neurosurgery, 134, 3, 1, 8, 13 Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Impact of cortical hemodynamic failure on both subsequent hemorrhagic stroke and effect of bypass surgery in hemorrhagic moyamoya disease: a supplementary analysis of the Japan Adult Moyamoya Trial.
Jun C Takahashi, Takeshi Funaki, Kiyohiro Houkin, Satoshi Kuroda, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
Journal of neurosurgery, 134, 3, 1, 6, 13 Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Constant current stimulation may improve apraxia of eyelid opening induced by deep brain stimulation
Eguchi K, Yabe I, Shirai S, Iwata I, Matsushima M, Yamazaki K, Hamauchi S, Sasamori T, Seki T, Houkin K, Sasaki H
Interdisciplinary Neurosurgery, 19, 100565, 100565, Mar. 2020, [Peer-reviewed]
English, Scientific journal

Discovery of a new pyrimidine synthesis inhibitor eradicating glioblastoma-initiating cells.
Smile Echizenya, Yukiko Ishii, Satoshi Kitazawa, Tadashi Tanaka, Shun Matsuda, Eriko Watanabe, Masao Umekawa, Shunsuke Terasaka, Kiyohiro Houkin, Tomohisa Hatta, Tohru Natsume, Yoshimasa Maeda, Shin-Ichi Watanabe, Shinji Hagiwara, Toru Kondo
Neuro-oncology, 22, 2, 229, 239, 20 Feb. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Glioblastoma-initiating cells (GICs) comprise a tumorigenic subpopulation of cells that are resistant to radio- and chemotherapies and are responsible for cancer recurrence. The aim of this study was to identify novel compounds that specifically eradicate GICs using a high throughput drug screening approach. METHODS: We performed a cell proliferation/death-based drug screening using 10 560 independent compounds. We identified dihydroorotate dehydrogenase (DHODH) as a target protein of hit compound 10580 using ligand-fishing and mass spectrometry analysis. The medical efficacy of 10580 was investigated by in vitro cell proliferation/death and differentiation and in vivo tumorigenic assays. RESULTS: Among the effective compounds, we identified 10580, which induced cell cycle arrest, decreased the expression of stem cell factors in GICs, and prevented tumorigenesis upon oral administration without any visible side effects. Mechanistic studies revealed that 10580 decreased pyrimidine nucleotide levels and enhanced sex determining region Y-box 2 nuclear export by antagonizing the enzyme activity of DHODH, an essential enzyme for the de novo pyrimidine synthesis. CONCLUSION: In this study, we identified 10580 as a promising new drug against GICs. Given that normal tissue cells, in particular brain cells, tend to use the alternative salvage pathway for pyrimidine synthesis, our findings suggest that 10580 can be used for glioblastoma therapy without side effects.Key Points1.  Chemical screening identified 10580 as a novel GIC-eliminating drug that targets DHODH, an essential enzyme for the de novo pyrimidine synthesis pathway. 2. Compound 10580 induced cell cycle arrest, apoptosis, and differentiation in GICs.

Crossed cerebellar diaschisis as an indicator of severe cerebral hyperperfusion after direct bypass for moyamoya disease.
Haruto Uchino, Ken Kazumata, Masaki Ito, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
Neurosurgical review, 44, 1, 599, 605, 19 Feb. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Association of the FGFR1 mutation with spontaneous hemorrhage in low-grade gliomas in pediatric and young adult patients.
Yukitomo Ishi, Shigeru Yamaguchi, Kanako C Hatanaka, Michinari Okamoto, Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
Journal of neurosurgery, 134, 3, 1, 9, 14 Feb. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: The authors aimed to investigate genetic alterations in low-grade gliomas (LGGs) in pediatric and young adult patients presenting with spontaneous hemorrhage. METHODS: Patients younger than 30 years of age with a pathological diagnosis of World Health Organization (WHO) grade I or II glioma and who had undergone treatment at the authors' institution were retrospectively examined. BRAF V600E, FGFR1 N546/K656, IDH1 R132, IDH2 R172, and KIAA1549-BRAF (K-B) fusion genetic alterations were identified, and the presence of spontaneous tumoral hemorrhage was recorded. RESULTS: Among 66 patients (39 with WHO grade I and 27 with grade II tumors), genetic analysis revealed K-B fusion in 18 (27.3%), BRAF V600E mutation in 14 (21.2%), IDH1/2 mutation in 8 (12.1%), and FGFR1 mutation in 4 (6.1%). Spontaneous hemorrhage was observed in 5 patients (7.6%); 4 of them had an FGFR1 mutation and 1 had K-B fusion. Univariate analysis revealed a statistically significant association of an FGFR1 mutation and a diencephalic location with spontaneous hemorrhage. Among 19 diencephalic cases including the optic pathway, hypothalamus, and thalamus, an FGFR1 mutation was significantly associated with spontaneous hemorrhage (p < 0.001). Four FGFR1 mutation cases illustrated the following results: 1) a 2-year-old female with pilomyxoid astrocytoma (PMA) harboring the FGFR1 K656E mutation presented with intraventricular hemorrhage (IVH); 2) a 6-year-old male with PMA harboring FGFR1 K656E and D652G mutations presented with intratumoral hemorrhage (ITH); 3) a 4-year-old female with diffuse astrocytoma harboring FGFR1 K656M and D652G mutations presented with IVH; and 4) a young adult patient with pilocytic astrocytoma with the FGFR1 N546K mutation presented with delayed ITH and IVH after 7 years of observation. CONCLUSIONS: Although the mechanism remains unclear, the FGFR1 mutation is associated with spontaneous hemorrhage in pediatric and young adult LGG.

Induction of large cerebral aneurysms by intraperitoneal administration of β-aminopropionitrile fumarate in male rats.
Yusuke Shimoda, Naoki Nakayama, Takuya Moriwaki, Takeo Abumiya, Masahito Kawabori, Kota Kurisu, Masayuki Gekka, Masaaki Hokari, Yasuhiro Ito, Kiyohiro Houkin
Journal of neurosurgical sciences, 04 Feb. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: It is necessary and useful to obtain an experimental model which steadily and rapidly induces aneurysms for investigation of the pathogenesis of cerebral aneurysm. We attempted to examine whether intraperitoneal administration of β-aminopropionitrile fumarate (BAPN-F) with additional treatments of induced hypertension and hemodynamic stress could steadily and rapidly induce aneurysms in male rats. METHODS: Seven-week-old male Sprague-Dawley rats pretreated with ligation of left common carotid and bilateral posterior renal arteries were administrated BAPN-F intraperitoneally. Induction rate and size of aneurysms was investigated with varying dose and duration of BAPN-F administration (low dose; 400 mg/kg/week for 4 or 8 weeks and high dose; 2800 mg/kg/week for 8 or 12 weeks). RESULTS: Induction rate in the high-dose groups was significantly higher (p<0.01) than that in the low-dose groups. Making comparisons between 8 and 12 weeks of the high-dose groups, while there was no difference in induction rate (8 weeks; 85.2% vs 12 weeks; 76.9%), aneurysmal size was larger in 12 weeks (8 weeks; 127.5 μm, vs 12 weeks; 181.7 μm in terms of median) but lethal intrathoracic hemorrhage was increased in 12 weeks (8 weeks; 7.4% vs 12 weeks; 30.8%). Induction rate of large aneurysm was 22.2% and 30.8% in 8 and 12 weeks of the high-dose groups, respectively. CONCLUSIONS: High-dose BAPN-F administration can cause high-frequency aneurysmal induction. Although there was the difference in size and mortality rate based on administration duration, intraperitoneal administration of 2800 mg/kg/week BAPN-F for 8 weeks would be suitable for aneurysmal induction.

Reversible Cerebral Angiopathy after Viral Infection in a Pediatric Patient with Genetic Variant of RNF213.
Ikuma Echizenya, Kikutaro Tokairin, Masahito Kawabori, Ken Kazumata, Kiyohiro Houkin
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 29, 2, 104549, 104549, Feb. 2020, [Peer-reviewed], [International Magazine]
English, Ring finger protein (RNF) 213 is known as a susceptibility gene for moyamoya disease (MMD), which is characterized by bilateral carotid folk stenosis. Cerebral angiopathy after viral infection has been known to present angiographical appearance resembling MMD, however its pathogenesis and genetic background are not well known. We report a case of reversible cerebral angiopathy after viral infection in a pediatric patient with genetic variant of RNF213 mutation. The patient had developed a severe headache after hand, foot, and mouth disease. Magnetic resonance imaging and magnetic resonance angiography (MRA) performed 2-3 weeks after disease onset revealed bilateral carotid folk stenosis and an old cerebral infarction in the left putamen. The patient's headache spontaneously resolved and the follow-up MRA showed a complete spontaneous resolution of the arterial stenosis after 9 months. We were able to determine genetic predisposition to angiopathy by identifying the RNF213 c.14576G>A (rs112735431, p.R4859K) mutation. Based on the present case, we hypothesize that an RNF213 variant might play an important role for the onset of postviral cerebral angiopathy.

Complication rate, cure rate, and long-term outcomes of microsurgery for intracranial dural arteriovenous fistulae: a multicenter series and systematic review.
Taku Sugiyama, Naoki Nakayama, Satoshi Ushikoshi, Ken Kazumata, Michinari Okamoto, Masaki Ito, Toshiya Osanai, Yusuke Shimoda, Kazuki Uchida, Daisuke Shimbo, Yasuhiro Ito, Katsuyuki Asaoka, Toshitaka Nakamura, Satoshi Kuroda, Kiyohiro Houkin
Neurosurgical review, 44, 1, 435, 450, 02 Jan. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

ラット脊髄損傷モデルにおける骨髄間葉系幹細胞シートによる治療効果の検証
山崎和義, 川堀真人, 川堀真人, 関俊隆, 高宮宗一朗, 寳金清博, 寳金清博
日本脳神経外傷学会プログラム・抄録集, 43rd, 2020

膠芽腫の治療成績:MEPを中心とした術中モニタリングによる術後performance status温存の重要性
山口秀, 茂木洋晃, 伊師雪友, 岡本迪成, 澤谷亮佑, 寳金清博
日本脳腫瘍の外科学会プログラム・抄録集, 25th, 2020

Long-Term Clinical Outcome and Prognosis After Thrombectomy in Patients With Concomitant Malignancy.
Sogo Oki, Masahito Kawabori, Sumire Echizenya, Yusuke Shimoda, Daisuke Shimbo, Toshiya Osanai, Kazuki Uchida, Kiyohiro Houkin
Frontiers in neurology, 11, 572589, 572589, 2020, [International Magazine]
English, Scientific journal

Evaluation of Novel Stereotactic Cannula for Stem Cell Transplantation against Central Nervous System Disease.
Masahito Kawabori, Aki Tanimori, Shinri Kitta, Hideo Shichinohe, Kiyohiro Houkin
Stem cells international, 2020, 4085617, 4085617, 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Cell therapy for central nervous system (CNS) disorders is beginning to prove its safety and efficiency. Intraparenchymal transplantation can be an option for cell delivery; however, one concern regarding this method is that the transplantation cannula may cause additional brain injuries. These include vessel damage, which results in brain hemorrhage, and clogging of the cannula by brain debris and/or cell clusters, which requires replacement of the cannula or forced injection causing jet flow of the cell suspension. We compared cannulas for cell delivery used in clinical trials, the Pittsburg and Mizuho cannulas, to a newly designed one, MK01, to assess their usability. MK01 has a spherical-shaped tip with a fan-like open orifice on the side of the cannula, which prevents vessel damage, clogging of brain debris, and jet flow phenomenon. We compared the extent of rat cervical and abdominal arterial damage with the cannula, the amount of debris in the cannula, the force needed to cause jet flow, and cell viability. While the viability of cells passed through the cannulas was almost the same among cannulas (approximately 95%), the Pittsburg cannula caused cervical arterial injury and subsequent hemorrhage, as it required a significantly smaller force to penetrate the arterial wall. Moreover, the Pittsburg cannula, but not the Mizuho and MK01 cannulas, showed high frequency of brain debris in the needle tip (approximately 80%) after brain puncture. While jet flow of the injection liquid was observed even when using smaller forces in the Pittsburg and Mizuho cannulas, MK01 constantly showed low jet flow occurrence. Thus, MK01 seems to be safer than the previously reported cannulas, although further investigation is necessary to validate its safety for clinical use.

FTY720 (Fingolimod) Ameliorates Brain Injury through Multiple Mechanisms and is a Strong Candidate for Stroke Treatment.
Zifeng Wang, Masahito Kawabori, Kiyohiro Houkin
Current medicinal chemistry, 27, 18, 2979, 2993, 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, FTY720 (Fingolimod) is a known sphingosine-1-phosphate (S1P) receptor agonist that exerts strong anti-inflammatory effects and was approved as the first oral drug for the treatment of multiple sclerosis by the US Food and Drug Administration (FDA) in 2010. FTY720 is mainly associated with unique functional "antagonist" and "agonist" mechanisms. The functional antagonistic mechanism is mediated by the transient down-regulation and degradation of S1P receptors on lymphocytes, which prevents lymphocytes from entering the blood stream from the lymph node. This subsequently results in the development of lymphopenia and reduces lymphocytic inflammation. Functional agonistic mechanisms are executed through S1P receptors expressed on the surface of various cells including neurons, astrocytes, microglia, and blood vessel endothelial cells. These functions might play important roles in regulating anti-apoptotic systems, modulating brain immune and phagocytic activities, preserving the Blood-Brain-Barrier (BBB), and the proliferation of neural precursor cells. Recently, FTY720 have shown receptor-independent effects, including intracellular target bindings and epigenetic modulations. Many researchers have recognized the positive effects of FTY720 and launched basic and clinical experiments to test the use of this agent against stroke. Although the mechanism of FTY720 has not been fully elucidated, its efficacy against cerebral stroke is becoming clear, not only in animal models, but also in ischemic stroke patients through clinical trials. In this article, we review the data obtained from laboratory findings and preliminary clinical trials using FTY720 for stroke treatment.

Simplified Dural Reconstruction Procedure Using Biocompatible Polyglycolic Acid Felt with Autologous Abdominal Fat Grafts after a Transpetrosal Approach.
Shigeru Yamaguchi, Shunsuke Terasaka, Michinari Okamoto, Yukitomo Ishi, Hiroaki Motegi, Hiroyuki Kobayashi, Kiyohiro Houkin
World neurosurgery, 132, e710-e715, E715, Dec. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Inflammation promotes progression of thrombi in intracranial thrombotic aneurysms.
Hime Suzuki, Takeshi Mikami, Tomoaki Tamada, Ryo Ukai, Yukinori Akiyama, Akinori Yamamura, Kiyohiro Houkin, Nobuhiro Mikuni
Neurosurgical review, 43, 6, 1565, 1573, 04 Nov. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Development of Hemorrhage-prone Anastomoses in Asymptomatic Moyamoya Disease-A Comparative Study with Japan Adult Moyamoya Trial.
Shusuke Yamamoto, Takeshi Funaki, Miki Fujimura, Jun C Takahashi, Haruto Uchino, Kiyohiro Houkin, Teiji Tominaga, Susumu Miyamoto, Satoshi Kuroda
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 28, 11, 104328, 104328, Nov. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Association of cognitive function with cerebral blood flow in children with moyamoya disease.
Ken Kazumata, Kikutaro Tokairin, Taku Sugiyama, Masaki Ito, Haruto Uchino, Toshiya Osanai, Masahito Kawabori, Naoki Nakayama, Kiyohiro Houkin
Journal of neurosurgery. Pediatrics, 1, 7, 11 Oct. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: The cognitive effects of main cerebral artery occlusive lesions are unclear in children with moyamoya disease (MMD). The authors aimed to investigate cognitive function in the presurgical phase of pediatric patients with MMD with no apparent brain lesions. METHODS: In this prospective, observational, single-center study, 21 children (mean age 10 ± 3.0 years, range 5-14 years) diagnosed with MMD at Hokkaido University Hospital between 2012 and 2018 were enrolled. A cross-sectional evaluation of intellectual ability was performed using the Wechsler Intelligence Scale for Children-Fourth Edition at the initial diagnosis. rCBF was measured using [123I] N-isopropyl p-iodoamphetamine/SPECT. The associations among clinical factors, disease severity, regional cerebral blood flow (rCBF), and intelligence test scores were also examined. RESULTS: The mean full-scale intelligence quotient (FIQ) was 101.8 ± 12.5 (range 76-125) in children with no apparent brain lesions. A significant difference in the intelligence scale index score was observed, most frequently (42.9%) between working memory index (WMI) and verbal comprehension index (VCI; VCI - WMI > 11 points). Regional CBF was significantly reduced both in the left and right medial frontal cortices (left: 61.3 ± 5.3 ml/100 g/min, right 65.3 ± 5.3 ml/100 g/min; p < 0.001) compared to the cerebellum (77.8 ± 6.8 ml/100 g/min). There was a significant association of rCBF in the left dorsolateral prefrontal cortex (DLPFC) with FIQ (r = 0.46, p = 0.034), perceptual reasoning index (PRI; r = 0.44, p = 0.045), and processing speed index (PSI; r = 0.44, p = 0.045). There was an association between rCBF of the left medial frontal cortex and PSI (r = 0.49, p = 0.026). Age of onset, family history, ischemic symptoms, and angiographic severity were not associated with poor cognitive performance. CONCLUSIONS: Although average intellectual ability was not reduced in children with MMD, the association of reduced rCBF in the left DLPFC and medial frontal cortex with FIQ, PRI, and PSI suggests mild cognitive dysfunction due to cerebral hypoperfusion.

A Rare Case of Idiopathic Temporal Muscle Abscess in a Nine-month-old Infant.
Sogo Oki, Masahito Kawabori, Hiroaki Motegi, Shigeru Yamaguchi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
Internal medicine (Tokyo, Japan), 58, 18, 2699, 2702, 15 Sep. 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Temporal muscle abscess in children usually occurs from acute otitis media, and rapid progression and concomitant infectious disease often make it easy to diagnose. We report a rare case of a nine-month-old infant who showed right temporal mass with no evidence of infection. Computed tomography showed an osteolytic round mass, and magnetic resonance imaging revealed heterogenous enhancement with a high apparent diffusion coefficient. Malignant tumor was first suspected, but an open biopsy revealed the swelling to be temporal muscle abscess. It should be noted that temporal abscess may mimic the features of a malignant tumor, and multiple examinations should be performed for an accurate diagnosis.

A Pilot Study on Measuring Tissue Motion During Carotid Surgery Using Video-Based Analyses for the Objective Assessment of Surgical Performance.
Taku Sugiyama, Toshitaka Nakamura, Yasuhiro Ito, Kikutaro Tokairin, Ken Kazumata, Naoki Nakayama, Kiyohiro Houkin
World journal of surgery, 43, 9, 2309, 2319, Sep. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: The 'gentle' handling of tissue (i.e., 'respect for tissue') is a fundamental aspect of surgical performance and learning. To date, there have been no methodological assessments that quantitatively measure 'gentleness.' Therefore, the aims of this study were (1) to propose a novel metric for gentle surgical maneuvers, (2) to validate the feasibility of this methodology, and (3) to explore safer surgical techniques through this methodology. METHODS: Using surgical video-based motion software, the motion of the carotid artery around plaque was analyzed and quantified during a carotid endarterectomy. Kinematic parameters (minimum and maximum acceleration, and maximum and mean velocity) were compared among the surgical tasks and techniques, as well as between novice and expert surgeons. RESULTS: The surgical tasks of dissecting the common carotid artery, passing the proximal vessel loops, and ligating vessels showed the highest absolute values of kinematic parameters. Dissections perpendicular to the line of the internal carotid artery tended to show higher kinematic parameters than those in the parallel direction, with blunt dissections typically higher than sharp dissections. The kinematic parameters of novice surgeons were significantly higher than those of experts, and receiver operating curve analysis showed a strong discriminative power. CONCLUSION: This study shows that tissue motion parameters could be a novel and feasible surrogate marker for the objective assessment on the 'gentleness' of surgical performance. Future studies should be performed to further elucidate the relationship on the direct correlation between tissue kinematic data and clinical outcomes or surgical adverse events.

Cost Effectiveness of Drive and Retrieve System in Hokkaido for Acute Ischemic Stroke Patient Treatment Using Geographic Information System.
Yasuhiro Morii, Toshiya Osanai, Tomoki Ishikawa, Kensuke Fujiwara, Takumi Tanikawa, Kiyohiro Houkin, Eiichi Kobayashi, Katsuhiko Ogasawara
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 28, 8, 2292, 2301, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND AND PURPOSE: Although endovascular thrombectomy combined with recombinant tissue-type plasminogen activator is effective for treatment of acute ischemic stroke, regional disparities in implementation rates of those treatments have been reported. Drive and retrieve system, where a qualified neurointerventionist travels to another primary stroke center for endovascular thrombectomy, has been practiced in parts of Hokkaido, Japan. This study aims to simulate the cost effectiveness of the drive and retrieve system, which can be a method to enhance equality and cost effectiveness of treatments for acute ischemic stroke. MATERIALS AND METHODS: The number of patients who had acute ischemic stroke in 2015 is estimated. Those patients are generated according to the population distribution, and thereafter patient transport time is analyzed in the 3 scenarios (1) 60-minute drive scenario, (2) 90-minute drive scenario, in which the drive and retrieve system operates within 60-minute or 90-minute driving distance (3) without the system, using geographic information system. Incremental cost-effectiveness rate, quality-adjusted life years, and medical and nursing care costs are estimated from the analyzed transport time. FINDINGS: The incremental cost-effectiveness rate by implementing the system was dominant. Cost reductions of $213,190 in 60-minute drive scenario, and $247,274 in the 90-minute scenario were expected, respectively. Such benefits are the most significant in Soya, Emmon, Rumoi, and Kamikawahokubu medical areas. CONCLUSIONS: The drive and retrieve system could enhance regional equality and cost effectiveness of ischemic stroke treatments in Hokkaido, which can be achieved using existing resources. Further studies are required to clarify its cost effectiveness from hospital perspective.

Characteristics of Moyamoya Disease Based on National Registry Data in Japan.
Yoko Sato, Ken Kazumata, Eiji Nakatani, Kiyohiro Houkin, Yasuhiro Kanatani
Stroke, 50, 8, 1973, 1980, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Investigation of the Neuropathic Pain Caused by Syringomyelia Associated with Chiari I Malformation.
Toshitaka Seki, Shuji Hamauchi, Masayoshi Yamazaki, Kazutoshi Hida, Shunsuke Yano, Kiyohiro Houkin
Asian spine journal, 13, 4, 648, 653, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Study Design: Retrospective cohort study. Purpose: To investigate the correlation between the syrinx morphology and neuropathic pain caused by syringomyelia associated with Chiari I malformation. Overview of Literature: Neuropathic pain caused by syringomyelia is refractory and markedly impairs the patient. Methods: We examined 24 patients with neuropathic pain caused by syringomyelia associated with Chiari I malformation. We statistically analyzed the illness duration and age at surgery between patients with and without neuropathic pain. Additionally, we classified the morphology of the syringes into deviated (D), enlarged (E), central (C), and bulkhead (B) types using T2-weighted axial imaging. Moreover, we investigated the correlation between syrinx morphology and neuropathic pain. A Mann-Whitney U-test was performed to compare between the presence or absence of neuropathic pain and the presence or absence of type D syringes. Results: The median age at surgery was 27.5 years, and the median illness duration was 24 months. Among the 24 patients, 11 had preoperative neuropathic pain, one of which was free of neuropathic pain during the final follow-up period. Among patients with neuropathic pain, the syringes' preoperative morphology was type D in nine patients and types E and C in one patient each. No patient exhibited type B morphology. Among patients without neuropathic pain, the preoperative morphology of the syringes was type D in three patients, type E in seven patients, and types C and B in two patients each. For types D and E, a correlation between neuropathic pain and syrinx morphology was observed. Moreover, type D was associated with significant neuropathic pain in both preoperative and postoperative states. Conclusions: This study showed a correlation between the morphological features of the syringes and the occurrence of neuropathic pain in patients with syringomyelia associated with Chiari I malformation.

Efficacy of 'drive and retrieve' as a cooperative method for prompt endovascular treatment for acute ischemic stroke.
Toshiya Osanai, Yasuhiro Ito, Satoshi Ushikoshi, Takeshi Aoki, Masahito Kawabori, Kensuke Fujiwara, Katsuhiko Ogasawara, Kikutaro Tokairin, Katsuhiko Maruichi, Naoki Nakayama, Ken Kazumata, Kota Ono, Kiyohiro Houkin
Journal of neurointerventional surgery, 11, 8, 757, 761, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Outcomes of endovascular treatment for acute ischemic stroke depend on the time interval from onset to reperfusion. Although the centralized 'mothership' method is considered preferable, the required transportation time increases the risk that a patient with a stroke may not receive intravenous or endovascular therapy. In contrast, 'drive and retrieve' describes a system wherein doctors from comprehensive stroke centers travel to primary stroke centers and provide endovascular treatment for acute ischemic stroke. OBJECTIVE: To describe the drive and retrieve system and verify the effects of this new collaboration on outcomes in patients with acute ischemic stroke among facilities. METHODS: This non-randomized, single-arm study retrospectively analyzed patients who met the inclusion criteria for endovascular treatment provided through a drive and retrieve system. Among the 122 patients treated by this system, we analyzed the time of onset to recanalization as the primary outcome. We also analyzed the efficacy of the drive and retrieve system using geographic information system analysis. RESULTS: The median time from onset to recanalization was 229 min (IQR 170-307 min, 95% CI 201 to 252 min). The upper limit of the 95% CI for the time from onset to recanalization was shorter than the median times reported in two previous trials. Geographic information system analysis revealed an upward trend in the population coverage rate in each secondary medical area after the drive and retrieve method was introduced. CONCLUSION: The drive and retrieve method may be an effective form of cooperation between facilities located within 1 hour of a comprehensive stroke center.

Contrecoup Injury-Induced Middle Meningeal Arteriovenous Fistula Detected by Time-of-Flight Magnetic Resonance Angiography and Magnetic Resonance Arterial Spin Labeling: Case Report and Review of the Literature.
Kikutaro Tokairin, Toshiya Osanai, Ken Kazumata, Ryosuke Sawaya, Kiyohiro Houkin
World neurosurgery, 127, 79, 84, Jul. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Middle meningeal arteriovenous fistula (MM-AVF) is rare; however, it will sometimes be followed by intracranial hemorrhage or progressive symptoms caused by abnormal shunt flow. Radiological examination and endovascular treatment of this condition have recently advanced; thus, we have described the pathogenesis, clinical features, and appropriate diagnostic and therapeutic management of MM-AVF. We also reviewed the reported data of the past 35 years, including 30 cases of MM-AVF. CASE DESCRIPTION: We report the case of 24-year-old man who had presented with right tinnitus who had experienced previous head trauma on the opposite side to the tinnitus ear. Time-of-flight magnetic resonance angiography and magnetic resonance arterial spin labeling findings were suggestive of MM-AVF, and catheter angiography confirmed MM-AVF with shunt flow draining into the cavernous sinus. Endovascular transarterial embolization was performed, and the MM-AVF was embolized successfully using detachable coils and n-butyl-2-cyanoacrylate. The tinnitus disappeared completely immediately after the treatment. CONCLUSIONS: MM-AVF is caused, not only by coup injury, but also by contrecoup injury. Time-of-flight magnetic resonance angiography and magnetic resonance arterial spin labeling are useful for detecting MM-AVF. Endovascular transarterial embolization is an effective and safe treatment.

Correlation between magnetic resonance imaging characteristics and BRAF alteration status in individuals with optic pathway/hypothalamic pilocytic astrocytomas.
Yukitomo Ishi, Shigeru Yamaguchi, Michiharu Yoshida, Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
Journal of neuroradiology = Journal de neuroradiologie, 48, 4, 266, 270, 20 Jun. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Dual antiplatelet therapy using cilostazol for secondary prevention in patients with high-risk ischaemic stroke in Japan: a multicentre, open-label, randomised controlled trial.
Kazunori Toyoda, Shinichiro Uchiyama, Takenori Yamaguchi, J Donald Easton, Kazumi Kimura, Haruhiko Hoshino, Nobuyuki Sakai, Yasushi Okada, Kortaro Tanaka, Hideki Origasa, Hiroaki Naritomi, Kiyohiro Houkin, Keiji Yamaguchi, Masanori Isobe, Kazuo Minematsu
The Lancet. Neurology, 18, 6, 539, 548, Jun. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Although dual antiplatelet therapy with aspirin and clopidogrel reduces early recurrence of ischaemic stroke, with long-term use this type of therapy is no longer effective and the risk of bleeding increases. Given that cilostazol prevents stroke recurrence without increasing the incidence of serious bleeding compared with aspirin, we aimed to establish whether dual antiplatelet therapy involving cilostazol is safe and appropriate for long-term use. METHODS: In a multicentre, open-label, randomised controlled trial across 292 hospitals in Japan, patients with high-risk non-cardioembolic ischaemic stroke identified on MRI were randomly assigned to two groups in a 1:1 ratio to receive monotherapy with either oral aspirin (81 or 100 mg, once per day) or clopidogrel (50 or 75 mg, once per day) alone, or a combination of cilostazol (100 mg, twice per day) with aspirin or clopidogrel. Randomisation was done centrally (using block randomisation with a block size of six per each participating hospital) through a web-based registration system and was done by EPS Corporation. The patients were required to have at least 50% stenosis of a major intracranial or extracranial artery or two or more of the vascular risk factors. Trial medication was continued for half a year or longer, for a maximum of 3·5 years. The primary efficacy outcome was the rate of first recurrence of symptomatic ischaemic stroke. Safety outcomes were severe or life-threatening bleeding; any adverse events; serious adverse events; and any bleeding events. Efficacy analyses were done in the intention-to-treat population and safety analyses were done in the as-treated population. This trial was registered with ClinicalTrials.gov (number NCT01995370) and UMIN Clinical Trials Registry (number 000012180). FINDINGS: Participants were recruited from Dec 13, 2013, to March 31, 2017. 932 patients assigned to the dual therapy group and 947 patients assigned to the monotherapy group were included in the intention-to-treat analysis. The trial was stopped after the enrolment of 1884 patients of an anticipated 4000 patients because of the delay in recruitment. Ischaemic stroke recurred in 29 (3%) of 932 patients (annualised rate 2·2%) on dual therapy including cilostazol and 64 (7%) of 947 patients (annualised rate 4·5%) on monotherapy during a median 1·4 years follow-up (hazard ratio [HR] 0·49, 95% CI 0·31-0·76, p=0·0010). Severe or life-threatening bleeding occurred in eight patients (annualised rate 0·6%) on dual therapy and 13 patients (annualised rate 0·9%) on monotherapy (HR 0·66, 95% CI 0·27-1·60, p=0·35). Occurrence of any type of adverse event was similar between the groups (255 [28%] of 910 patients in the dual therapy group vs 219 [24%] of 921 patients in the monotherapy group); as was occurrence of serious adverse events (87 [10%] vs 142 [15%]) and bleeding events (38 [4%] vs 33 [4%]). Gastrointestinal bleeding, which affected nine (<1%) of 910 patients in the monotherapy group and nine (<1%) of 921 patients in the dual therapy group, was the most common type of bleeding. INTERPRETATION: The combination of cilostazol with aspirin or clopidogrel had a reduced incidence of ischaemic stroke recurrence and a similar risk of severe or life-threatening bleeding compared with treatment with aspirin or clopidogrel alone in patients at high risk for recurrent ischaemic stroke. FUNDING: Otsuka Pharmaceutical.

Identification of PEPT2 as an important candidate molecule in 5-ALA-mediated fluorescence-guided surgery in WHO grade II/III gliomas.
Chongxian Hou, Shigeru Yamaguchi, Yukitomo Ishi, Shunsuke Terasaka, Hiroyuki Kobayashi, Hiroaki Motegi, Kanako C Hatanaka, Kiyohiro Houkin
Journal of neuro-oncology, 143, 2, 197, 206, Jun. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, PURPOSE: 5-aminolevulinic acid (5-ALA) fluorescence-guided surgery (FGS) appears to be a promising treatment for glioma. However, 5-ALA-mediated fluorescence cannot always be detected in grade II/III gliomas. We hypothesized that gene expression patterns in the Protoporphyrin IX (PpIX) synthesis pathway may be associated with intraoperative fluorescence status of grade II/III gliomas, and then attempted to identify the key molecule of 5-ALA-mediated fluorescence. METHODS: Using 50 surgically obtained specimens, which were diagnosed as grade II and III gliomas, we analyzed gene expression within the PpIX synthesis pathway to identify candidate molecules according to intraoperative 5-ALA-mediated fluorescence status. The most likely candidate gene was selected and confirmed by protein expression analysis. To evaluate the biological function of the molecule in PpIX synthesis, functional analysis was performed using specific, small interference (si)RNA in the SW-1783 human grade III glioma cell line. RESULTS: Among the genes involved in the porphyrin synthesis pathway, the mRNA expression of Peptide transporter 2 (PEPT2) in FGS fluorescence-positive gliomas was significantly higher than that in fluorescence-negative gliomas. Protein expression of PEPT2 was also significantly higher in the fluorescence-positive gliomas, which was confirmed by western blot analysis and immunofluorescence analysis. The siRNA-mediated downregulation of the mRNA and protein expression of PEPT2 led to decreased PpIX fluorescence intensity, as confirmed by fluorescence spectrum analysis. CONCLUSIONS: The results suggest PEPT2 is an important candidate molecule in 5-ALA-mediated FGS in grade II/III gliomas. As the overexpression of PEPT2 was associated with higher PpIX fluorescence intensity, PEPT2 may improve fluorescence-guided resection in grade II/III gliomas.

Strategy and effect of repeat bypass surgery for anterior/posterior circulation in refractory moyamoya disease.
Haruto Uchino, Daina Kashiwazaki, Naoki Akioka, Masaki Koh, Naoya Kuwayama, Kiyohiro Houkin, Satoshi Kuroda
Journal of neurosurgery, 132, 6, 1, 11, 31 May 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: In this study the authors aimed to describe clinical features, surgical techniques, and long-term outcomes of repeat bypass surgery required for a certain subset of patients with moyamoya disease. METHODS: The authors retrospectively reviewed a total of 22 repeat bypass surgeries for 20 patients (age range 1-69 years) performed during the last 20 years at their institutions. The patients were classified into 2 groups. Group A included 10 patients who underwent repeat bypass surgery for anterior circulation due to insufficient revascularization on the ipsilateral side. Group B included 10 patients who underwent repeat bypass surgery for posterior circulation due to the involvement of the posterior cerebral artery (PCA) after successful initial surgery for anterior circulation. RESULTS: Preoperative symptoms included headache in 3 patients, transient ischemic attack in 10, cerebral infarction in 3, and intracranial hemorrhage in 4 patients. Intervals between the initial bypass surgery and repeat bypass surgery were 0.3-30 years (median 3 years). In group A, superficial temporal artery to middle cerebral artery (MCA) anastomosis and indirect bypass were performed on 7 hemispheres. Only indirect bypass was performed on 3 hemispheres because of the lack of suitable donor or recipient arteries. In group B, occipital artery (OA) to PCA anastomosis and indirect bypass were conducted on 4 hemispheres, and OA-MCA anastomosis and indirect bypass on 1 hemisphere. Only indirect bypass was conducted on 7 hemispheres because of the lack of suitable recipient arteries. All 22 repeat bypass surgeries were successfully conducted. During follow-up periods (median 4 years), none of the patients suffered repeat stroke except 1 patient who died of recurrent intracerebral hemorrhage 3 years after repeat bypass surgery for anterior circulation. CONCLUSIONS: Repeat bypass surgery was feasible and effective to reduce further incidence of headache attack, transient ischemic attack, and ischemic/hemorrhagic stroke in moyamoya disease patients. Through precise radiological analysis, surgical procedures should be planned to yield maximal therapeutic effects.

Intrinsic development of choroidal and thalamic collaterals in hemorrhagic-onset moyamoya disease: case-control study of the Japan Adult Moyamoya Trial.
Miki Fujimura, Takeshi Funaki, Kiyohiro Houkin, Jun C Takahashi, Satoshi Kuroda, Yasutake Tomata, Teiji Tominaga, Susumu Miyamoto
Journal of neurosurgery, 130, 5, 1453, 1459, 01 May 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: This study was performed to identify the angiographic features of hemorrhagic-onset moyamoya disease (MMD) in comparison with those of patients with ischemic-onset MMD. METHODS: This case-control study compared the data set of the Japan Adult Moyamoya (JAM) Trial with the angiographic data of adult patients with ischemic-onset MMD. The authors analyzed angiograms obtained at onset, classifying the collaterals into 3 subtypes: lenticulostriate anastomosis, thalamic anastomosis, and choroidal anastomosis. They then compared the extent of these collaterals, as indicated by the collateral development grade from 0 to 2 in each subtype, between the JAM Trial group and the ischemic-onset group. They also compared the involvement of the posterior cerebral artery (PCA) and Suzuki's angiographic staging between each group. RESULTS: Among 89 ischemic-onset patients, 103 symptomatic hemispheres in 80 patients were analyzed and compared with 75 hemorrhagic hemispheres from the JAM Trial. The hemorrhagic-onset patients showed a significantly higher proportion of thalamic anastomosis (p = 0.043) and choroidal anastomosis (< 0.001), as indicated by grade 2 in each subtype, compared with ischemic-onset patients. Suzuki's angiographic staging was significantly higher in the hemorrhagic group (< 0.038). There was no difference in the extent of lenticulostriate anastomosis and PCA involvement between the groups. CONCLUSIONS: In adult MMD, the characteristic pattern of the abnormal vascular networks at the base of the brain is different between each onset type. In light of the more prominent development of thalamic and choroidal anastomosis in the JAM Trial group in the present study, development of these collaterals, especially the choroidal collateral extending beyond the lateral ventricle, may play a critical role in hemorrhagic presentation in MMD.Clinical trial registration no. C000000166 (http://www.umin.ac.jp/ctr/index.htm).

Mollaret Meningitis with a High Level of Cytokines in the Cerebrospinal Fluid Successfully Treated by Indomethacin.
Masahito Kawabori, Kota Kurisu, Yoshimasa Niiya, Yuzuru Ohta, Shoji Mabuchi, Kiyohiro Houkin
Internal medicine (Tokyo, Japan), 58, 8, 1163, 1166, 15 Apr. 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, A rare case of Mollaret meningitis characterized by four recurrent episodes of aseptic meningitis during a three-year period is reported. The patient showed a high fever and severe headache accompanied by a high level of cerebrospinal fluid (CSF) cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). The symptoms and high CSF cytokines were resolved immediately after introducing indomethacin treatment. Reactivation of the latent virus is considered to be the cause of this rare disease, and indomethacin is believed to inhibit the periodic abnormal generation of eicosanoid in the brain, resulting in a reduction in the fever and subsequent inflammation.

Estimation of the number of feeding arteries of spinal arteriovenous malformations by using three-dimensional digital subtraction angiography.
Soichiro Takamiya, Toshiya Osanai, Toshitaka Seki, Noriyuki Fujima, Kazutoshi Hida, Takeshi Asano, Kazuyoshi Yamazaki, Shuji Hamauchi, Toru Sasamori, Kota Ono, Shunsuke Terasaka, Kiyohiro Houkin
European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 28, 4, 842, 848, Apr. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, PURPOSE: Spinal angiography is the gold standard for evaluation or diagnosis of spinal arteriovenous malformations (AVMs). However, some feeding arteries might be overlooked when multiple feeders exist. This study aimed to retrospectively review cases of spinal intra-dural AVMs, which were identified by three-dimensional digital subtraction angiography (3D-DSA), and attempted to estimate the number of feeding arteries. METHODS: We retrospectively reviewed patients with spinal intra-dural AVMs who underwent 3D-DSA at Hokkaido University Hospital from January 2005 to December 2016. We selected 9 patients in whom we could obtain data of multi-planar reconstruction of 3D-DSA. We measured the computed tomography (CT) values of feeding arteries and draining veins. The CT values represented the averages of maximum CT values of 5 continuous axial slices. The ratio of the CT value of feeders to that of drainers (F/D ratio) was calculated. The correlation between the F/D ratio and the number of feeders was examined with Pearson's correlation coefficient. RESULTS: The average number of feeders was 2.3 (1-4), and the number of feeders was significantly positively correlated with the F/D ratio (r = 0.855, P = .003). CONCLUSIONS: We conclude that the number of feeding arteries of spinal intra-dural AVMs can be estimated by using the F/D ratio obtained from 3D-DSA. These slides can be retrieved under Electronic Supplementary Material.

Effect of choroidal collateral vessels on de novo hemorrhage in moyamoya disease: analysis of nonhemorrhagic hemispheres in the Japan Adult Moyamoya Trial.
Takeshi Funaki, Jun C Takahashi, Kiyohiro Houkin, Satoshi Kuroda, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
Journal of neurosurgery, 132, 2, 408, 414, 08 Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: Following hemorrhagic stroke in moyamoya disease, de novo intracranial hemorrhage can occur in the previously unaffected nonhemorrhagic hemisphere. In the present analysis the authors intended to determine whether the presence in the nonhemorrhagic hemisphere of choroidal collateral vessels, which have been the focus of attention as a source of bleeding, affects the risk of de novo hemorrhage. METHODS: The subject of focus of the present cohort study was the nonhemorrhagic hemispheres of adult patients with hemorrhagic moyamoya disease enrolled in the Japan Adult Moyamoya Trial and allocated to the nonsurgical arm. The variable of interest was the presence of choroidal collaterals (also termed choroidal anastomoses), identified with baseline angiography and represented by a connection (anastomosis) between the anterior or posterior choroidal arteries and the medullary arteries. The outcome measure was de novo hemorrhage during the 5-year follow-up period, assessed in all nonhemorrhagic hemispheres. The incidence of de novo hemorrhage in the collateral-positive and -negative groups was compared. RESULTS: Choroidal collaterals were present in 15 of 36 (41.7%) nonhemorrhagic hemispheres analyzed. The overall annual risk of de novo hemorrhage was 2.0%. Three de novo hemorrhages occurred in the collateral-positive group, whereas no hemorrhage occurred in the collateral-negative group. The annual risk of de novo hemorrhage was significantly higher in the collateral-positive group than in the collateral-negative group (5.8% per year vs 0% per year; p = 0.017). All hemorrhage sites corresponded to the distribution of choroidal collaterals. CONCLUSIONS: The present preliminary results suggest that the presence of choroidal collaterals affects the risk of de novo hemorrhage in the nonhemorrhagic hemisphere, subject to verification in larger studies. Further studies are needed to determine the optimal treatment strategy for nonhemorrhagic hemispheres and asymptomatic patients.

Rivaroxaban administration after acute ischemic stroke: The RELAXED study
Masahiro Yasaka, Kazuo Minematsu, Kazunori Toyoda, Etsuro Mori, Teruyuki Hirano, Toshimitsu Hamasaki, Hiroshi Yamagami, Takehiko Nagao, Shinichi Yoshimura, Shinichiro Uchiyama, Takenori Yamaguchi, Yasushi Okada, Satoshi Okuda, Kazumi Kimura, Norio Tanahashi, Yasuo Terayama, Yoichiro Hashimoto, Yasuhiro Hasegawa, Kiyohiro Houkin, Masayasu Matsumoto, Kazuo Kitagawa, Masahiro Yasaka, Ken Nagata, Shigeru Nogawa, Yoshiaki Kumon, Takeshi Kimura, Yutaka Furukawa, Tomohiro Sakamoto, Makoto Sakai, Kohsuke Kudo, Toshinori Hirai, Shotai Kobayashi
PLOS ONE, 14, 2, Feb. 2019, [Peer-reviewed]
English, Scientific journal

High rebleeding risk associated with choroidal collateral vessels in hemorrhagic moyamoya disease: analysis of a nonsurgical cohort in the Japan Adult Moyamoya Trial.
Takeshi Funaki, Jun C Takahashi, Kiyohiro Houkin, Satoshi Kuroda, Shigekazu Takeuchi, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
Journal of neurosurgery, 130, 2, 337, 673, 01 Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVEChoroidal collateral vessels typical of moyamoya disease have received attention as a potential bleeding source. The authors' previous angiographic cross-sectional analysis suggested a possible association between choroidal collaterals and posterior hemorrhage, indicating a high risk for rebleeding. The present longitudinal analysis is intended to determine whether choroidal collaterals are a predictor of rebleeding in hemorrhagic moyamoya disease.METHODSThe Japan Adult Moyamoya Trial group designed an ancillary cohort study using 5-year follow-up data on 37 patients included in the nonsurgical arm of the original randomized controlled trial and compared the rebleeding rate of those with and those without choroidal collaterals, represented by the connection between the anterior or posterior choroidal arteries and the medullary arteries. An expert panel determined whether a choroidal collateral was present in each patient through the measurement of baseline angiography studies. The rebleeding rate comparison was adjusted for age, diagnosis of hypertension, and involvement of the posterior cerebral artery.RESULTSChoroidal collaterals were present in 21 patients (56.8%). The rebleeding rate was 13.1% per year in the collateral-positive group as compared with 1.3% in the negative group (p = 0.008, log-rank test). The adjusted hazard ratio for rebleeding in the collateral-positive group relative to the negative group remained statistically significant (HR 11.10, 95% CI 1.37-89.91). Radiographic assessment of the collateral-positive group revealed good correspondence between the distribution of collaterals and rebleeding sites.CONCLUSIONSResults of this study suggest that choroidal collaterals are a bleeding source with a high risk for hemorrhagic recurrence and a predictor of rebleeding in hemorrhagic moyamoya disease.

Long-Term Evaluation of Combination Treatment of Single Agent HD-MTX Chemotherapy up to Three Cycles and Moderate Dose Whole Brain Irradiation for Primary CNS Lymphoma.
Hiroyuki Kobayashi, Shigeru Yamaguchi, Hiroaki Motegi, Sadahiro Kaneko, Shogo Endou, Rikiya Onimaru, Shunsuke Terasaka, Kiyohiro Houkin
Journal of chemotherapy (Florence, Italy), 31, 1, 35, 41, Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, High-dose methotrexate (HD-MTX)-based chemotherapy in combination with whole brain radiotherapy (WBRT) has been a common therapy for primary central nervous system lymphoma (PCNSL). The aim of this study was to evaluate the survival benefit of a minimized cycle of HD-MTX monotherapy prior to WBRT. A maximum of three cycles of HD-MTX was combined with a WBRT dose of 30 Gy and an additional localized boost was administered where remnant was observed. A total of 54 patients with newly diagnosed PCNSL were enrolled in this study. The objective response rate for HD-MTX was 80% and the median overall survival was 58.4 months. Responders to HD-MTX demonstrated better survival than patients with resistance. The concentration of MTX in serum and cerebrospinal fluid was not related the chemotherapeutic response. This study demonstrated the efficacy of HD-MTX prior to WBRT and indicated that three cycles of HD-MTX monotherapy may be sufficient in combination with radiotherapy.

SONODYNAMIC THERAPY FOR MALIGNANT GLIOMA USING 220-KHZ TRANSCRANIAL MAGNETIC RESONANCE IMAGING-GUIDED FOCUSED ULTRASOUND AND 5-AMINOLEVULINIC ACID
Michiharu Yoshida, Hiroyuki Kobayashi, Shunsuke Terasaka, Shogo Endo, Shigeru Yamaguchi, Hiroaki Motegi, Rachmilevitch Itay, Shiji Suzuki, Omer Brokman, Yeruham Shapira, Kenji Moriyama, Yumiko Kawase, Toshiaki Akahane, Yasutaka Kato, Hajime Kamada, Kiyohiro Houkin
ULTRASOUND IN MEDICINE AND BIOLOGY, 45, 2, 526, 538, Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

ラット亜急性期脊髄損傷モデルにおける骨髄間葉系幹細胞シートによる治療効果の検証
山崎和義, 川堀真人, 関俊隆, 七戸秀夫, 高宮宗一郎, 濱内祝嗣, 寳金清博, 寳金清博
日本分子脳神経外科学会プログラム・抄録集, 20th, 2019

脳梗塞亜急性期に対する自家骨髄間質細胞の脳内投与 第一相治験:RAINBOW研究
川掘真人, 七戸秀夫, 黒田敏, 寳金清博
脳循環代謝(Web), 31, 1, 2019

ラット脊髄損傷モデルにおけるFTY720の治療効果の検証
山崎和義, 川堀真人, 関俊隆, 濱内祝嗣, 寳金清博
日本脊髄外科学会プログラム・抄録集, 34th, 2019

成人脊髄係留症候群:当院12症例の検討
高宮宗一朗, 関俊隆, 濱内祝嗣, 山崎和義, 飛彈一利, 小柳泉, 寳金清博
日本脊髄外科学会プログラム・抄録集, 34th, 2019

もやもや病(ウイリス動脈輪閉塞症)の診断,治療に関する研究
寳金清博
もやもや病(ウイリス動脈輪閉塞症)の診断、治療に関する研究 平成30年度 総括研究報告書(Web), 2019

もやもや病(ウイリス動脈輪閉塞症)の診断,治療に関する研究 日本もやもやレジストリ構築の進捗状況
数又研, 寳金清博
もやもや病(ウイリス動脈輪閉塞症)の診断、治療に関する研究 平成30年度 総括研究報告書(Web), 2019

術前にCTガイド下経皮的嚢胞穿刺を行ったperineural cystの一例
濱内祝嗣, 関俊隆, 森田亮, 木村輔, 阿保大介, 寳金清博
日本脊髄外科学会プログラム・抄録集, 34th, 2019

下錐体静脈洞サンプリングにおけるアクセスルートMR venography
東海林菊太郎, 長内俊也, 藤間憲幸, 牛越聡, 茂木洋晃, 伊師雪友, 寳金清博
脳血管内治療(Web), 4, Supplement, 2019

北海道での理想的な血栓回収療法提供体制の確立に向けた取り組み
長内俊也, 東海林菊太郎, 今井哲秋, 寳金清博, 小笠原克彦
脳血管内治療(Web), 4, Supplement, 2019

脊髄神経膠腫の分子診断と予後
伊師雪友, 関俊隆, 高宮宗一郎, 山崎和義, 濱内祝嗣, 山口秀, 寳金清博, 飛騨一利, 石田雄介, 小田義崇, 田中仲哉, 茂木洋晃, 岡本迪成
日本脳腫瘍学会プログラム・抄録集, 37th, 2019

Postoperative Intracerebral Hemorrhage After Combined Revascularization Surgery in Moyamoya Disease: Profiles and Clinical Associations.
Kikutaro Tokairin, Ken Kazumata, Haruto Uchino, Masaki Ito, Kota Ono, Ryota Tatezawa, Takafumi Shindo, Masahito Kawabori, Naoki Nakayama, Kiyohiro Houkin
World neurosurgery, 120, e593-e600, e600, Dec. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: In combined revascularization surgery for patients with moyamoya disease, intracerebral hemorrhage (ICH) during the postoperative acute phase is a rarely observed but severe complication. Its clinical features remain unclear because of its low incidence rate. The aim of this study was to clarify the clinical characteristics of immediate postoperative ICH. METHODS: The frequency, onset timing, and hematoma location of patients who demonstrated immediate postoperative ICH were investigated in 201 consecutive surgeries performed in 134 patients. Associations between immediate postoperative ICH and demographics, clinical presentation type, perioperative blood pressure (BP), and neuroimaging data were analyzed. RESULTS: Postoperative ICH was observed in 6 cases (2.99%; mean age, 46.0 ± 7.6 years). The onset timing of ICH was within 24 hours after surgery in most patients (83.3%). Hematomas were located at the subcortical lesion beneath the anastomosed cortex (n = 5) and caudate head (n = 1). Three cases (50.0%) required hematoma evacuation. A higher age at surgery was associated with postoperative ICH (P = 0.046). In adult cases (132 surgeries, 65.7%), hemorrhagic presentation at onset (P = 0.0027) and an increase in BP from pre- to postoperative stage (systolic BP increase: P = 0.0058, diastolic BP increase: P = 0.0274) were significantly associated with postoperative ICH. CONCLUSIONS: The results suggest that older patients, with hemorrhagic presentation and greater postoperative BP increase, should be carefully managed to avoid postoperative ICH. Immediate hematoma evacuation may be effective in preventing devastating outcomes after postoperative ICH.

The validity of the Acute Stroke Assessment using rapid Pseudo-continuous arterial spin labeling (ASAP-ASL) method for acute thrombectomy.
Daisuke Oura, Masahito Kawabori, Yoshimasa Niiya, Motoyuki Iwasaki, Shinpei Satoh, Takumi Yokohama, Shoji Mabuchi, Kiyohiro Houkin
Journal of neurosurgical sciences, 21 Nov. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Recent clinical trials demonstrated the efficacy of thrombectomy for ischemic stroke against acute large vessel occlusion (LVO). To overcome the problem with excessive examination time for diagnosis of cerebral perfusion and/or the use of contrast agent to determine penumbra, we adopted a new magnetic resonance imaging technique named Acute Stroke Assessment using rapid Pseudo-continuous arterial spin labeling (ASAP-ASL) method. METHODS: The study included healthy volunteers and clinical patients. The signal to noise ratio (SNR) and acquisition time were compared with various numbers of signal average (NSA) of rapid pseudo-continuous arterial spin labeling (pCASL) using the 10-mm thick slice width and narrow scan range focusing the level of basal ganglia by healthy volunteers. After applying clinically acceptable protocol for ASAP-ASL, we then checked image qualities and an accuracy of the method by comparing with the angiographical imaging obtained from the clinical patients regarding the degree of consistency. RESULTS: NSA were compared between two and fourteen, and 10 NSA was decided to be introduced for clinical use (1 minutes and 17 second) for obtaining clinically acceptable image, which was shorter than the time required for ordinary whole brain pCASL (approximately 5 minutes). In the clinical study, the occlusion site estimated by ASAP-ASL showed high correlation with that of digital subtraction angiography (κ = 0.63-0.79). CONCLUSIONS: ASAP-ASL method requires approximately one minutes to obtain clinically relevant brain perfusion imaging which can successfully identify ischemic region in LVO patients.

Intraoperative real-time identification of abnormal vessels within the bright field by superselective arterial injection of saline and its slow-motion recording using a high frame rate digital camera during surgical treatment of spinal arteriovenous shunts: technical note.
Shuji Hamauchi, Toshiya Osanai, Toshitaka Seki, Masahito Kawabori, Michinari Okamoto, Kazutoshi Hida, Kiyohiro Houkin
Journal of neurosurgery. Spine, 29, 5, 576, 581, 01 Nov. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, The authors describe a novel method of observing blood flow in abnormal vessels with slow-motion video during surgical treatment of spinal arteriovenous shunts. The method is based on the use of superselective angiography with saline for visualizing abnormal vessels in bright field and commercially available high frame rate digital camera for recording slow-motion video.

Electrocardiogram-Triggered Angiography Non-Contrast-Enhanced (TRANCE) Imaging to Assess Access Route Before Diagnostic Cerebral Angiography.
Toshiya Osanai, Ken Kazumata, Satoshi Kobayashi, Noriyuki Fujima, Kota Kurisu, Yuusuke Shimoda, Kiyohiro Houkin
World neurosurgery, 119, 237, 241, Nov. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Electrocardiogram (ECG)-triggered angiography non-contrast-enhanced (TRANCE) imaging is useful for investigating peripheral vessel diseases; however, its efficacy for access route assessment in cerebral angiography has yet to be reported. Therefore we aimed to evaluate the efficacy of TRANCE imaging in the assessment of the access route before diagnostic subtraction angiography for cerebral vascular disorders. METHODS: TRANCE imaging was performed in all patients undergoing catheter angiography for disease diagnosis at our institute between April 2014 and March 2015. This study included 31 patients (14 men, 17 women) and investigated potential changes in the planned puncture site before and during the procedure (including the reason for the change) as our main outcome. RESULTS: TRANCE was successfully conducted in all of the patients recruited. TRANCE images led to an alteration in the approach vessel for 5 cases. The mean procedure time for angiography was <60 minutes. The median total number of catheters and guidewires used during catheter angiography was 1 (range: 1-3) and 1 (range: 1-2), respectively. There were no catheter angiography-related complications. CONCLUSIONS: TRANCE imaging before diagnostic angiography is useful because it allows detailed assessment of the access route. This practice may reduce procedure time, thus resulting in fewer complications.

Direct common carotid artery puncture for acute thrombectomy against ischemic stroke.
Masahito Kawabori, Toshiya Osanai, Shuho Goto, Motoyuki Iwasaki, Yoshimasa Niiya, Shoji Mabuchi, Kiyohiro Houkin
Journal of neurosurgical sciences, 62, 5, 612, 614, Oct. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Identification of plaque location using intraoperative indocyanine green during carotid endarterectomy for patient with near occlusion.
Masahito Kawabori, Yoshimasa Niiya, Motoyuki Iwasaki, Shoji Mabuchi, Kiyohiro Houkin
Journal of neurosurgical sciences, 65, 4, 397, 401, 25 Sep. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Trends of Antiplatelet Therapy for the Management of Moyamoya Disease in Japan: Results of a Nationwide Survey.
Oki K, Katsumata M, Izawa Y, Takahashi S, Suzuki N, Houkin K, Research Committee on, Spontaneous Occlusion of Circle of Willis, Moyamoya disease
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 27, 12, 3605, 3612, Sep. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: The efficacy and safety of antiplatelet drugs in the treatment of moyamoya disease remain unclear. This study reports results of a nationwide survey conducted in 2016 on the trends of antiplatelet therapy for moyamoya disease in Japan. METHODS: Data were obtained through questionnaires related to treatment policies regarding antiplatelet drugs from each specialized stroke management department of 765 hospitals in Japan. Data were also compared between experienced facilities (defined as facilities managing more than 10 cases per year) and those less experienced (not more than 10 cases per year) to determine experts' opinion. RESULTS: Of the 389 departments in 375 hospitals that responded, 330 departments provided medical care for moyamoya disease. Regarding ischemic stroke, numerous departments considered the use of antiplatelet drugs "in principle" (218 departments). After surgery for ischemic moyamoya disease, the use of antiplatelet drugs for a certain period of time was the most popular opinion (74 departments). Regarding asymptomatic moyamoya disease, majority departments reported no use of APDs "in principle" (256 departments). The experienced facilities reported "no use of antiplatelet drugs" more frequently than those less experienced for treating asymptomatic moyamoya disease. In moyamoya disease, aspirin was the most commonly used antiplatelet drugs followed by cilostazol and clopidogrel. CONCLUSIONS: This survey revealed details of treatment policies, and the selection of antiplatelet drugs widely varied across facilities. Further prospective studies are necessary to improve the current unclear situation regarding the use of antiplatelet drugs for the management of moyamoya disease.

Novel Hemoglobin-Based Oxygen Carrier Bound With Albumin Shows Neuroprotection With Possible Antioxidant Effects.
Masayuki Gekka, Takeo Abumiya, Teruyuki Komatsu, Ryosuke Funaki, Kota Kurisu, Daisuke Shimbo, Masato Kawabori, Toshiya Osanai, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
Stroke, 49, 8, 1960, 1968, Aug. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Background and Purpose- A hemoglobin-albumin cluster, 1 core of hemoglobin covalently bound with 3 shell albumins, designated as HemoAct was developed as a hemoglobin-based oxygen carrier. We aim to investigate neuroprotection by HemoAct in transient cerebral ischemia and elucidate its underlying mechanisms. Methods- Male rats were subjected to 2-hour transient middle cerebral artery occlusion and were then administered HemoAct transarterially at the onset of reperfusion. Neurological and pathological findings were examined after 24 hours of reperfusion to identify neuroprotection by HemoAct. Intermittent measurements of cortical blood flow and oxygen content were performed, and a histopathologic analysis was conducted on rats during the early phase of reperfusion to assess the therapeutic mechanism of HemoAct. In addition, the antioxidant effects of HemoAct were examined in hypoxia/reoxygenation-treated rat brain microvascular endothelial cells. Results- Neurological deterioration, infarct and edema development, and the activation of MMP-9 (matrix metalloprotease-9) and lipid peroxidation after 24 hours of reperfusion were significantly ameliorated by the HemoAct treatment. Reductions in blood flow and tissue partial oxygen pressure in the cortical penumbra after 6 hours of reperfusion were significantly ameliorated by the HemoAct treatment. The histopathologic analysis of the cortical penumbra revealed that HemoAct in HemoAct-treated rats showed superior microvascular perfusion with the mitigation of microvascular narrowing changes than autologous erythrocytes in nontreated rats. Although HemoAct extravasated into the ischemic core with serum protein, it did not induce an increase in serum extravasation or reactive oxygen species production in the ischemic core. In vitro experiments with rat brain microvascular endothelial cells revealed that HemoAct significantly suppressed cellular reactive oxygen species production in hypoxia/reoxygenation-treated cells, similar to albumin. Conclusions- HemoAct exerted robust neuroprotection in transient cerebral ischemia. Superior microvascular perfusion with an oxygen delivery capability and possible antioxidant effects appear to be the underlying neuroprotective mechanisms.

Myelocystocele mimicking myelomeningocele: A case report and review of the literature.
Takamiya S, Seki T, Ikeda T, Shinada SI, Hamauchi S, Terasaka S, Houkin K
World neurosurgery, 119, 172, 175, Aug. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Myelocystoceles, which are classified as closed neural tube defects, are usually covered by skin and rarely complicated by hydrocephalus. We encountered an unusual case of a terminal myelocystocele with hydrocephalus with clinical characteristics of a myelomeningocele. CASE DESCRIPTION: Severe hydrocephalus and a lumbosacral lesion were detected in the fetus of a gravid 34-year-old woman. Cesarean section was performed at 37 weeks. The neonate presented with a lumbosacral mass with a partial skin defect. As myelomeningocele was suspected, the neonate underwent surgery on the day of birth. The intraoperative findings pointed to a myelocystocele rather than a myelomeningocele. After insertion of a ventriculoperitoneal shunt, the neonate was discharged without any neurologic deficits. CONCLUSIONS: Presence of skin abnormalities, hydrocephalus, and lumbosacral mass strongly suggests a diagnosis of myelomeningocele. However, such cases should be differentiated from myelocystocele, especially when associated with severe hydrocephalus.

Circulating miRNome profiling in Moyamoya disease-discordant monozygotic twins and endothelial microRNA expression analysis using iPS cell line.
Uchino H, Ito M, Kazumata K, Hama Y, Hamauchi S, Terasaka S, Sasaki H, Houkin K
BMC medical genomics, 11, 1, 72, 72, Aug. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Moyamoya disease (MMD) is characterized by progressive stenosis of intracranial arteries in the circle of Willis with unknown etiology even after the identification of a Moyamoya susceptible gene, RNF213. Recently, differences in epigenetic regulations have been investigated by a case-control study in MMD. Here, we employed a disease discordant monozygotic twin-based study design to unmask potential confounders. METHODS: Circulating genome-wide microRNA (miRNome) profiling was performed in MMD-discordant monozygotic twins, non-twin-MMD patients, and non-MMD healthy volunteers by microarray followed by qPCRvalidation, using blood samples. Differential plasma-microRNAs were further quantified in endothelial cells differentiated from iPS cell lines (iPSECs) derived from another independent non-twin cohort. Lastly, their target gene expression in the iPSECs was analyzed. RESULTS: Microarray detected 309 plasma-microRNAs in MMD-discordant monozygotic twins that were also detected in the non-twin cohort. Principal component analysis of the plasma-microRNA expression level demonstrated distinct 2 groups separated by MMD and healthy control in the twin- and non-twin cohorts. Of these, differential upregulations of hsa-miR-6722-3p/- 328-3p were validated in the plasma of MMD (absolute log2 expression fold change (logFC) > 0.26 for the twin cohort; absolute logFC > 0.26, p < 0.05, and q < 0.15 for the non-twin cohort). In MMD derived iPSECs, hsa-miR-6722-3p/- 328-3p showed a trend of up-regulation with a 3.0- or higher expression fold change. Bioinformatics analysis revealed that 41 target genes of miR-6722-3p/- 328-3p were significantly down-regulated in MMD derived iPSECs and were involved in STAT3, IGF-1-, and PTEN-signaling, suggesting a potential microRNA-gene expression interaction between circulating plasma and endothelial cells. CONCLUSIONS: Our MMD-discordant monozygotic twin-based study confirmed a novel circulating microRNA signature in MMD as a potential diagnostic biomarker minimally confounded by genetic heterogeneity. The novel circulating microRNA signature can contribute for the future functional microRNA analysis to find new diagnostic and therapeutic target of MMD.

THE DEVELOPMENT AND EVALUATION OF A NEW SIMPLE MODIFIED ANEURYSM CLIP FOR SMALL ANIMAL SCI MODELS
Kazuyoshi Yamazaki, Toshitaka Seki, Masahito Kawabori, Syuji Hamauchi, Izumi Koyanagi, Kiyohiro Houkin
JOURNAL OF NEUROTRAUMA, 35, 16, A168, A168, Aug. 2018, [Peer-reviewed]
English

The prognostic improvement of add-on bevacizumab for progressive disease during concomitant temozolomide and radiation therapy in the patients with glioblastoma and anaplastic astrocytoma.
Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Michinari Okamoto, Hiroyuki Kobayashi, Kenji Hirata, Yoshitaka Oda, Shinya Tanaka, Shunsuke Terasaka, Kiyohiro Houkin
Journal of neurosurgical sciences, 64, 6, 502, 508, 09 Jul. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Interdisciplinary Prevention and Management of Wound-Related Complications in Extracranial-to-Intracranial Bypass Surgery
Rintaro Yokoyama, Takeshi Mikami, Ryo Ukai, Katsuya Komatsu, Yusuke Kimura, Hime Suzuki, Toshimi Honma, Toru Hirano, Tamotsu Saito, Ken Yamashita, Takatoshi Yotsuyanagi, Kiyohiro Houkin, Nobuhiro Mikuni
WORLD NEUROSURGERY, 115, 247, 253, Jul. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Development of moyamoya disease after non-herpetic acute limbic encephalitis: A case report
Yasuhiro Takahashi, Takeshi Mikami, Hime Suzuki, Katsuya Komatsu, Daisuke Yamamoto, Shun Shimohama, Kiyohiro Houkin, Shintaro Sugita, Tadashi Hasegawa, Nobuhiro Mikuni
Journal of Clinical Neuroscience, 53, 250, 253, Churchill Livingstone, 01 Jul. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Surgical outcomes of pediatric patients with asymptomatic tethered cord syndrome
Toshitaka Seki, Kazutoshi Hida, Shunsuke Yano, Kiyohiro Houkin
Asian Spine Journal, 12, 23, 551, 555, Korean Society of Spine Surgery, 01 Jun. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Treatment evaluation of acute stroke for using in regenerative cell elements (TREASURE) trial: Rationale and design.
Toshiya Osanai, Kiyohiro Houkin, Shinichiro Uchiyama, Kazuo Minematsu, Akihiko Taguchi, Shunsuke Terasaka
International journal of stroke : official journal of the International Stroke Society, 13, 4, 444, 448, Jun. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Rationale MultiStem® (HLM051) is one of the promising allogenic cell products for acute ischemic stroke with strong evidence. A previous phase 2 randomized, double-blind, placebo-controlled, multicenter dose-escalation trial showed the safety of MultiStem® for acute ischemic stroke, with a time window beyond that of rt-PA and endovascular thrombectomy. We aim to obtain stronger evidence and to show the efficacy of the MultiStem® for treatment of ischemic stroke. Sample size Estimated sample size is 220 (110 patients per group), which has 90% power at 5% significance level. Methods and design TREASURE is a randomized, double-blind, placebo-controlled, multicenter phase 2/3 trial. The trial will be done at 31 medical centers in Japan. Patients with acute ischemic stroke including motor or speech deficit defined by a National Institution of Health Stroke Scale (NIHSS) score of 8-20 at baseline will be randomized 1:1 to receive a single intravenous infusion of MultiStem® or placebo within 18-36 h of stroke onset. Study outcomes Primary outcome in this study is the proportion of patients with an excellent outcome at day 90 defined by the functional assessment. Trial registration ClinicalTrials.gov (NCT02961504). Conclusion The TREASURE trial will provide a novel treatment option and expand the therapeutic window for patients with stroke if the results are positive.

[18F]DPA-714 PET imaging shows immunomodulatory effect of intravenous administration of bone marrow stromal cells after transient focal ischemia.
Chengbo Tan, Songji Zhao, Kei Higashikawa, Zifeng Wang, Masahito Kawabori, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Naoyuki Ukon, Hironobu Yasui, Nagara Tamaki, Yuji Kuge, Hideo Shichinohe, Kiyohiro Houkin
EJNMMI research, 8, 1, 35, 35, 02 May 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: The potential application of bone marrow stromal cell (BMSC) therapy in stroke has been anticipated due to its immunomodulatory effects. Recently, positron emission tomography (PET) with [18F]DPA-714, a translocator protein (TSPO) ligand, has become available for use as a neural inflammatory indicator. We aimed to evaluate the effects of BMSC administration after transient middle cerebral artery occlusion (MCAO) using [18F]DPA-714 PET. The BMSCs or vehicle were administered intravenously to rat MCAO models at 3 h after the insult. Neurological deficits, body weight, infarct volume, and histology were analyzed. [18F]DPA-714 PET was performed 3 and 10 days after MCAO. RESULTS: Rats had severe neurological deficits and body weight loss after MCAO. Cell administration ameliorated these effects as well as the infarct volume. Although weight loss occurred in the spleen and thymus, cell administration suppressed it. In both vehicle and BMSC groups, [18F]DPA-714 PET showed a high standardized uptake value (SUV) around the ischemic area 3 days after MCAO. Although SUV was increased further 10 days after MCAO in both groups, the increase was inhibited in the BMSC group, significantly. Histological analysis showed that an inflammatory reaction occurred in the lymphoid organs and brain after MCAO, which was suppressed in the BMSC group. CONCLUSIONS: The present results suggest that BMSC therapy could be effective in ischemic stroke due to modulation of systemic inflammatory responses. The [18F]DPA-714 PET/CT system can accurately demonstrate brain inflammation and evaluate the BMSC therapeutic effect in an imaging context. It has great potential for clinical application.

Characteristics of Symptomatic Intracerebral Hemorrhage in Patient Receiving Direct Oral Anticoagulants: Comparison with Warfarin.
Masahito Kawabori, Yoshimasa Niiya, Motoyuki Iwasaki, Shoji Mabuchi, Hiroyuki Ozaki, Koji Matsubara, Kiyohiro Houkin
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 27, 5, 1338, 1342, May 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Direct oral coagulants (DOAC) have been shown to decrease the frequency of intracerebral hemorrhage (ICH) compared with warfarin. However, the precise characteristics, such as the size and locations of the hemorrhage, and outcome and onset time of ICH in patient taking DOAC are not fully elucidated. METHODS: We retrospectively analyzed the characteristics of symptomatic patients with ICH taking either DOAC or warfarin between January 2012 and December 2015. RESULTS: Out of 400 consecutive patients with ICH, 15 patients were DOAC-ICH and 24 patients were warfarin-ICH. DOAC-ICH was observed in 6 patients with 10 mg of rivaroxaban, 5 patients with 15 mg of rivaroxaban, and 1 patient with 10 mg of apixaban, 5 mg of apixaban, 30 mg of edoxaban, and 60 mg of edoxaban. Prothrombin time was well controlled in most of the warfarin-ICH patients (83.3%). The locations of ICH were similar in both groups; however, median ICH volume was significantly smaller in DOAC-ICH patients than in warfarin-ICH patients (P < .01) and ICH around basal ganglia seemed to show great difference between the groups. DOAC-ICH patients showed better neurological outcome at the time of discharge than warfarin patients (P < .01), and the ratio of good prognosis was significantly higher in the DOAC-ICH patients than in the warfarin-ICH patients (P < .01). The onset of warfarin-ICH was frequently observed in the morning and evening, whereas DOAC-ICH did not show any specific onset time. CONCLUSIONS: Patients with DOAC-ICH showed smaller ICH volume and better clinical outcomes than patients with warfarin-ICH, and DOAC-ICH did not show any specific onset peak.

Accelerating Cell Therapy for Stroke in Japan: Regulatory Framework and Guidelines on Development of Cell-Based Products.
Kiyohiro Houkin, Hideo Shichinohe, Koji Abe, Teruyo Arato, Mari Dezawa, Osamu Honmou, Nobutaka Horie, Yasuo Katayama, Kohsuke Kudo, Satoshi Kuroda, Tomohiro Matsuyama, Ichiro Miyai, Izumi Nagata, Kuniyasu Niizuma, Ken Sakushima, Masanori Sasaki, Norihiro Sato, Kenji Sawanobori, Satoshi Suda, Akihiko Taguchi, Teiji Tominaga, Haruko Yamamoto, Toru Yamashita, Toshiki Yoshimine
Stroke, 49, 4, e145-e152, e152, Apr. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Angiographic features of hemorrhagic moyamoya disease with high recurrence risk: a supplementary analysis of the Japan Adult Moyamoya Trial.
Takeshi Funaki, Jun C Takahashi, Kiyohiro Houkin, Satoshi Kuroda, Shigekazu Takeuchi, Miki Fujimura, Yasutake Tomata, Susumu Miyamoto
Journal of neurosurgery, 128, 3, 777, 784, Mar. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE In this paper, the authors set out to identify the angiographic features of moyamoya disease with posterior hemorrhage, which is a strong predictor of rebleeding. METHODS This cross-sectional study used the data set of the Japan Adult Moyamoya Trial (clinical trial registration no.: C000000166 [ www.umin.ac.jp/ctr/index.htm ]). The panel designed the ancillary measurement of angiography at onset, classifying the collateral vessels into 3 subtypes: lenticulostriate anastomosis, thalamic anastomosis, and choroidal anastomosis. The association between each collateral and the hemorrhage site (anterior vs posterior) was assessed in the hemorrhagic hemisphere by using multivariate adjustment for potential confounders, including age, sex, and involvement of the posterior cerebral artery (PCA). The association was confirmed through topographical analysis of bleeding points. RESULTS Among the 80 participants, 75 hemorrhagic hemispheres of 75 patients were analyzed. Lenticulostriate anastomosis was detected in 21 (28.0%) hemorrhagic hemispheres, thalamic anastomosis in 22 (29.3%), and choroidal anastomosis in 35 (46.7%). Choroidal anastomosis was a factor associated with posterior hemorrhage (OR 2.77 [95% CI 1.08-7.10], p = 0.034) and remained statistically significant after the multivariate adjustment (OR 2.66 [95% CI 1.00-7.07], p = 0.049). PCA involvement was also associated with posterior hemorrhage in both univariate and multivariate analyses. Topographical analysis revealed good correspondence between bleeding points associated with positive choroidal anastomosis and the anatomical distribution of the choroidal arteries, including the thalamus and the wall of the atrium. CONCLUSIONS Choroidal anastomosis and PCA involvement are characteristic of posterior hemorrhage in moyamoya disease. Choroidal anastomosis might be considered a potential source of posterior hemorrhage at high risk of rebleeding.

Adult-Onset Orbital Sinus Pericranii with T2 Hyperintensity Lesion: A Case Report
Toshiya Osanai, Kiyohiro Houkin
Case Reports in Neurology, 10, 1, 112, 117, S. Karger AG, 14 Feb. 2018, [Peer-reviewed]
English, Scientific journal

Factors influencing cerebral aneurysm obliteration and reliability of indocyanine green video-angiography
Masayuki Gekka, Naoki Nakayama, Haruto Uchino, Kiyohiro Houkin
Acta Neurochirurgica, 160, 2, 269, 276, Springer-Verlag Wien, 01 Feb. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke.
Michiyuki Miyamoto, Kentaro Nakamura, Hideo Shichinohe, Tomohiro Yamauchi, Masaki Ito, Hisayasu Saito, Masahito Kawabori, Toshiya Osanai, Tasuku Sasaki, Kiyohiro Houkin, Satoshi Kuroda
Stem cells international, 2018, 4829534, 4829534, 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke. However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits. We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke. BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo). A BMSC-RCP sponge construct was transplanted onto the ipsilateral intact neocortex 7 days after MCAo. A BMSC suspension or vehicle was transplanted into the ipsilateral striatum. Rat motor function was serially evaluated and histological analysis was performed 5 weeks after transplantation. The results showed that BMSCs could proliferate well in the RCP sponge and the BMSC-RCP sponge significantly promoted functional recovery, compared with the vehicle group. Histological analysis revealed that the RCP sponge provoked few inflammatory reactions in the host brain. Moreover, some BMSCs migrated to the peri-infarct area and differentiated into neurons in the BMSC-RCP sponge group. These findings suggest that the RCP sponge may be a promising candidate for animal protein-free scaffolds in cell therapy for ischemic stroke in humans.

国際臨床医学会 大きな第二歩へ
寳金清博, 寳金清博, 寳金清博, 寳金清博, 寳金清博
国際臨床医学会雑誌(Web), 2, 1, 2018

頭蓋内硬膜動静脈瘻に対する4D-MRAの検討
東海林菊太郎, 長内俊也, 藤間憲幸, 牛越聡, 寳金清博
脳血管内治療(Web), 3, Supplement, 2018

Accessibility to Tertiary Stroke Centers in Hokkaido, Japan: Use of Novel Metrics to Assess Acute Stroke Care Quality.
Kensuke Fujiwara, Toshiya Osanai, Eiichi Kobayashi, Takumi Tanikawa, Ken Kazumata, Kikutaro Tokairin, Kiyohiro Houkin, Katsuhiko Ogasawara
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 27, 1, 177, 184, Jan. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Both the accessibility and availability of stroke specialists are major determinants of patient outcomes following acute ischemic stroke (AIS). The purpose of this study was to implement novel metrics to assess the accessibility of tertiary stroke centers as well as to evaluate regional disparities in stroke specialists. METHODS: Using network analysis in a geographic information system, we calculated areas within 30- and 60-minute travel times to facilities providing intravenous recombinant tissue-type plasminogen activator and mechanical thrombectomy. We further evaluated the accessibility for the proportion of the population aged 65 years or older that resided outside of these areas. Uniformity in the geographical distribution of stroke specialists was then evaluated using optimal statistical analysis. RESULTS: Accessibility varied widely from region to region, with low accessibility being concentrated in rural areas with low population density. Accessibility to facilities providing mechanical thrombectomy was especially low, and 17.8% of elderly individuals lived ≥60 minutes from treatment facilities. In addition, the distribution of stroke specialists was uneven compared with the distribution of hospital beds and full-time medical doctors. CONCLUSION: The results of this study revealed regional disparities in the spatial accessibility to treatment facilities, as well as in the distribution of stroke specialists in Hokkaido. These findings provide useful information that could be employed to appropriately allocate resources toward the formation of a medical supply system for patients with AIS.

Intraoperative Visualization of a Spinal Arachnoid Cyst Using Pyoktanin Blue
Soichiro Takamiya, Toshitaka Seki, Kazuyoshi Yamazaki, Toru Sasamori, Kiyohiro Houkin
World Neurosurgery, 109, 18, 23, Elsevier Inc., 01 Jan. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Berlin Grading System Can Stratify the Onset and Predict Perioperative Complications in Adult Moyamoya Disease
Daina Kashiwazaki, Naoki Akioka, Naoya Kuwayama, Kiyohiro Houkin, Marcus Czabanka, Peter Vajkoczy, Satoshi Kuroda
NEUROSURGERY, 81, 6, 986, 991, Dec. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Superior Microvascular Perfusion of Infused Liposome-Encapsulated Hemoglobin Prior to Reductions in Infarctions after Transient Focal Cerebral Ischemia
Daisuke Shimbo, Takeo Abumiya, Kota Kurisu, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Hideki Nakamura, Hiroshi Shimuzu, Kiyohiro Houkin
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 26, 12, 2994, 3003, Dec. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Novel insights into symptomatology of moyamoya disease in pediatric patients: survey of symptoms suggestive of orthostatic intolerance
Haruto Uchino, Ken Kazumata, Masaki Ito, Naoki Nakayama, Kiyohiro Houkin
JOURNAL OF NEUROSURGERY-PEDIATRICS, 20, 5, 485, 488, Nov. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Diencephalic pediatric low-grade glioma harboring the BRAF V600E mutation presenting with various morphologies in sequential biopsy specimens
Yukitomo Ishi, Kanako C. Hatanaka, Shigeru Yamaguchi, Hiromi Fujita, Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
BRAIN TUMOR PATHOLOGY, 34, 4, 165, 171, Oct. 2017, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Double Crush of L5 Spinal Nerve Root due to L4/5 Lateral Recess Stenosis and Bony Spur Formation of Lumbosacral Transitional Vertebra Pseudoarticulation: A Case Report and Review.
Iwasaki M, Akiyama M, Koyanagi I, Niiya Y, Ihara T, Houkin K
NMC case report journal, 4, 4, 121, 125, Oct. 2017, [Peer-reviewed], [Domestic magazines]
English, We present a case of double-crushed L5 nerve root symptoms caused by inside and outside of the spinal canal with spur formation of the lumbosacral transitional vertebra (LSTV). A 78-year-old man presented with 7-year history of moderate paresis of his toe and left leg pain when walking. Magnetic resonance imaging (MRI) revealed spinal stenosis at the L3/4 and 4/5 spinal levels and he underwent wide fenestration of both levels. Leg pain disappeared and 6-min walk distance (6MWD) improved after surgery, however, the numbness in his toes increased and 6MWD decreased 9 months after surgery. Repeated MR and 3D multiplanar reconstructed computed tomography (CT) images showed extraforaminal impingement of the L5 root by bony spur of the left LSTV. He underwent second decompression surgery of the L5/S via the left sided Wiltse approach, resulting in the improvement of his symptoms. The impingement of L5 spinal nerve root between the transverse process of the fifth lumbar vertebra and the sacral ala is a rare entity of the pathology called "far-out syndrome (FOS)". Especially, the bony spur formation secondary to the anomalous articulation of the LSTV (LSPA) has not been reported. These articulations could be due to severe disc degeneration, following closer distance and contact between the transverse process and the sacral ala. To our knowledge, this is the first report describing a case with this pathology and may be considered in cases of failed back surgery syndromes (FBSS) of the L5 root symptoms.

Research on advanced intervention using novel bone marrOW stem cell (RAINBOW): a study protocol for a phase I, open-label, uncontrolled, dose-response trial of autologous bone marrow stromal cell transplantation in patients with acute ischemic stroke
Hideo Shichinohe, Masahito Kawabori, Hiroaki Iijima, Tuyoshi Teramoto, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Shunsuke Terasaka, Teruyo Arato, Kiyohiro Houkin
BMC NEUROLOGY, 17, 1, 179, 179, Sep. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Ten-Year Retrospective Study on the Management of Spinal Arteriovenous Lesions: Efficacy of a Combination of Intraoperative Digital Subtraction Angiography and Intraarterial Dye Injection
Toshiya Osanai, Kazutoshi Hida, Takeshi Asano, Toshitaka Seki, Toru Sasamori, Kiyohiro Houkin
WORLD NEUROSURGERY, 104, 841, 847, Aug. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Prevalence of and risk factors for enlarged perivascular spaces in adult patients with moyamoya disease
Tomoyoshi Kuribara, Takeshi Mikami, Katsuya Komatsu, Hime Suzuki, Hirofumi Ohnishi, Kiyohiro Houkin, Nobuhiro Mikuni
BMC NEUROLOGY, 17, 1, 149, 149, Aug. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Regional transarterial hypothermic infusion in combination with endovascular thrombectomy in acute ischaemic stroke with cerebral main arterial occlusion: protocol to investigate safety of the clinical trial
Kikutaro Tokairin, Toshiya Osanai, Takeo Abumiya, Ken Kazumata, Kota Ono, Kiyohiro Houkin
BMJ OPEN, 7, 8, e016502, Aug. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effect of Headache on the Pathologic Findings of Unruptured Cerebral Saccular Aneurysms
Masaaki Hokari, Naoki Nakayama, Yusuke Shimoda, Kiyohiro Houkin
WORLD NEUROSURGERY, 103, 431, 441, Jul. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Pathophysiological consideration of medullary streaks on FLAIR imaging in pediatric moyamoya disease
Hime Suzuki, Takeshi Mikami, Tomoyoshi Kuribara, Kazuhisa Yoshifuji, Katsuya Komatsu, Yukinori Akiyama, Hirofumi Ohnishi, Kiyohiro Houkin, Nobuhiro Mikuni
JOURNAL OF NEUROSURGERY-PEDIATRICS, 19, 5, 560, 566, May 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Efficacy and safety of non-suture dural closure using a novel dural substitute consisting of polyglycolic acid felt and fibrin glue to prevent cerebrospinal fluid leakage-A non-controlled, open-label, multicenterclinical trial-
Shunsuke Terasaka, Toshiaki Taoka, Satoshi Kuroda, Nobutaka Mikuni, Toru Nishi, Hiroyuki Nakase, Yukihiko Fujii, Yasuhiko Hayashi, Jun-ichi Murata, Ken-ichiro Kikuta, Toshihiko Kuroiwa, Sachie Shimokawa, Kiyohiro Houkin
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 28, 5, 69, 69, May 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Direct Anastomosis Using Occipital Artery for Additional Revascularization in Moyamoya Disease After Combined Superficial Temporal Artery-Middle Cerebral Artery and Indirect Bypass
Ken Kazumata, Hiroyasu Kamiyama, Hisayasu Saito, Katsuhiko Maruichi, Masaki Ito, Haruto Uchino, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
OPERATIVE NEUROSURGERY, 13, 2, 213, 222, Apr. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Surgical Outcome of Cerebral Aneurysm Clipping Treated with Immunosuppressants: Report of 11 Cases and Review of the Literature
Masaaki Hokari, Naoki Nakayama, Ken Kazumata, Toshiya Osanai, Hideo Shichinohe, Takeo Abumiya, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 57, 3, 122, 127, Mar. 2017, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Application of cell sheet technology to bone marrow stromal cell transplantation for rat brain infarct
Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Satoshi Kuroda
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 11, 2, 375, 381, Feb. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Development of Database and Genomic Medicine for von Hippel-Lindau Disease in Japan
Shunsaku Takayanagi, Akitake Mukasa, Hirofumi Nakatomi, Hiroshi Kanno, Jun-ichi Kuratsu, Ryo Nishikawa, Kazuhiko Mishima, Atushi Natsume, Toshihiko Wakabayashi, Kiyohiro Houkin, Shunsuke Terasaka, Masahiro Yao, Nobuo Shinohara, Taro Shuin, Nobuhito Saito
NEUROLOGIA MEDICO-CHIRURGICA, 57, 2, 59, 65, Feb. 2017, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Proposal for a Prospective Registry for Moyamoya Disease in Japan
Ken Kazumata, Masaki Ito, Haruto Uchino, Hiroshi Nishihara, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 57, 2, 66, 72, Feb. 2017, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Synergistic Interactions Between Direct and Indirect Bypasses in Combined Procedures: The Significance of Indirect Bypasses in Moyamoya Disease.
Uchino H, Kim JH, Fujima N, Kazumata K, Ito M, Nakayama N, Kuroda S, Houkin K
Neurosurgery, 80, 2, 201, 209, Feb. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal, Background: Whether additional indirect bypasses effectively contribute to revascularization in combined procedures remains unclear in patients with moyamoya disease. Objective: To evaluate the longitudinal changes associated with combined procedures while following up pediatric and adult patients long term and to assess whether any other clinical factors or hemodynamic parameters affected these changes to determine an optimal surgical strategy. Methods: We studied 58 hemispheres in 43 adults and 39 hemispheres in 26 children who underwent combined revascularization for moyamoya disease. To evaluate bypass development, we assessed the sizes of the superficial temporal artery and middle meningeal artery using magnetic resonance angiography. Multivariate analysis determined the effects of multiple variables on bypass development. Results: Indirect bypass (middle meningeal artery) development occurred in 95% and 78% of the pediatric and adult hemispheres, respectively. Of these, dual development of direct and indirect bypasses occurred in 54% of the pediatric hemispheres and in 47% of the adult hemispheres. Reciprocal superficial temporal artery regression occurred in 28% of the hemispheres during the transition from the postoperative acute phase to the chronic phase during indirect bypass development. Good indirect bypass development was associated with adult hemispheres at Suzuki stage 4 or greater (odds ratio, 7.4; 95% confidence interval, 1.4-39.4; P = .02). Disease onset type and preoperative hemodynamic parameters were not considered predictors for the development of surgical revascularization. Conclusion: Simultaneous direct and indirect bypass development was most frequently observed, regardless of patient age and hemodynamic status. Applying indirect bypass as an adjunct to direct bypass could maximize revascularization in adults and children.

Synergistic Interactions Between Direct and Indirect Bypasses in Combined Procedures: The Significance of Indirect Bypasses in Moyamoya Disease
Haruto Uchino, Jae-Hoon Kim, Noriyuki Fujima, Ken Kazumata, Masaki Ito, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
NEUROSURGERY, 80, 2, 201, 208, Feb. 2017, [Peer-reviewed]
English, Scientific journal

病院到着前の情報からtPA静注療法もしくは血栓回収療法を必要とする症例の予測因子に関する検討
東海林菊太郎, 東海林菊太郎, 長内俊也, 川堀真人, 川堀真人, 新谷好正, 寳金清博
脳血管内治療(Web), 2, Supplement, 2017

Functional bio-imaging
Hisayasu Saito, Michiyuki Miyamoto, Hideo Shichinohe, Kiyohiro Houkin, Satoshi Kuroda
Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials, 111, 119, Springer Japan, 01 Jan. 2017, [Peer-reviewed]
English, In book

Cell culture
Masaki Ito, Kiyohiro Houkin
Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials, 49, 72, Springer Japan, 01 Jan. 2017, [Peer-reviewed]
English, In book

In vivo cell tracking techniques for applications in central nervous system disorders
Taku Sugiyama, Satoshi Kuroda, Kiyohiro Houkin
Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials, 101, 110, Springer Japan, 01 Jan. 2017, [Peer-reviewed]
English, In book

Cell therapy for ischemic stroke with bone marrow stromal cells
Satoshi Kuroda, Hideo Shichinohe, Kiyohiro Houkin
Cell Therapy against Cerebral Stroke: Comprehensive Reviews for Translational Researches and Clinical Trials, 15, 25, Springer Japan, 01 Jan. 2017, [Peer-reviewed]
English, In book

Health-related quality of life in patients with spinal dural arteriovenous fistulae
Toru Sasamori, Kazutoshi Hida, Toshiya Osanai, Shunsuke Yano, Toshitaka Seki, Kiyohiro Houkin
NEUROSURGICAL REVIEW, 40, 1, 83, 86, Jan. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

[Utility of Intraoperative Catheter Myelography during Intrathecal Baclofen Pump Implantation:Report of 2 Cases].
Takamiya S, Sasamori T, Hamauchi S, Seki T, Kano T, Yabe I, Sasaki H, Houkin K
No shinkei geka. Neurological surgery, 45, 1, 53, 58, Jan. 2017, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, We report two patients in whom the intrathecal baclofen(ITB)catheter was located in the subdural space, although we had confirmed good outflow of spinal fluid from the spinal catheter. Patient 1 was a woman in her 30s with spastic quadriplegia due to subarachnoid hemorrhage. An ITB pump was implanted, and a good outflow of spinal fluid from the spinal catheter was observed during the surgery. Postoperatively, her spasticity did not improve. Catheter myelography revealed that the spinal catheter was located in the subdural space. Using intraoperative catheter myelography, we corrected the position of the catheter. Patient 2 was a man in his 20s diagnosed with adrenoleukodystrophy. An ITB therapy was performed to improve his spastic gait. Intraoperative catheter myelography showed that the spinal catheter was located in the subdural space, although there was good outflow of spinal fluid from the catheter. Our experience suggests that the outflow of spinal fluid alone should not be used to determine the location of the spinal catheter. Intraoperative catheter myelography is useful for the correct placement of the spinal catheter in the subarachnoid space.

Meandering flow void around the splenium in moyamoya disease
Takeshi Mikami, Tomoyoshi Kuribara, Katsuya Komatsu, Yusuke Kimura, Masahiko Wanibuchi, Kiyohiro Houkin, Nobuhiro Mikuni
NEUROLOGICAL RESEARCH, 39, 8, 702, 708, 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

A prospective, multicentre, single-arm clinical trial of bevacizumab for patients with surgically untreatable, symptomatic brain radiation necrosis†.
Motomasa Furuse, Naosuke Nonoguchi, Toshihiko Kuroiwa, Susumu Miyamoto, Yoshiki Arakawa, Jun Shinoda, Kazuhiro Miwa, Toshihiko Iuchi, Koji Tsuboi, Kiyohiro Houkin, Shunsuke Terasaka, Yusuke Tabei, Hideo Nakamura, Motoo Nagane, Kazuhiko Sugiyama, Mizuhiko Terasaki, Tatsuya Abe, Yoshitaka Narita, Nobuhito Saito, Akitake Mukasa, Kuniaki Ogasawara, Takaaki Beppu, Toshihiro Kumabe, Tadashi Nariai, Naohiro Tsuyuguchi, Eiji Nakatani, Shoko Kurisu, Yoko Nakagawa, Shin-Ichi Miyatake
Neuro-oncology practice, 3, 4, 272, 280, Dec. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Brain radiation necrosis (BRN) can be a complication of radiotherapy for primary and secondary brain tumors, as well as head and neck tumors. Since vascular endothelial growth factor (VEGF) is also a vascular permeability factor in the brain, bevacizumab, a humanized antibody that inhibits VEGF, would be expected to reduce perilesional edema that often accompanies BRN. METHODS: Patients with surgically untreatable, symptomatic BRN refractory to conventional medical treatments (eg, corticosteroid, anticoagulants, or hyperbaric oxygen therapy) were enrolled. We judged that a major cause of perilesional edema with a lesion-to-normal brain ratio ≤1.8 on 11C-methionine or ≤2.5 on 18F-boronophenylalanine PET was BRN, not tumor recurrence, and 6 cycles of biweekly bevacizumab (5 mg/kg) were administered. The primary endpoint was a ≥30% reduction from the patients' registration for perilesional edema continuing for ≥1 month. RESULTS: Of the 41 patients enrolled, 38 were fully eligible for the response assessment. The primary endpoint was achieved in 30 of the 38 (78.9%) patients at 3.0 months (median) after enrollment. Sixteen patients (42.1%) experienced improvement of their Karnofsy Performance Score. Corticosteroid use could be reduced in 29 patients (76.3%). Adverse events at grade ≥3 occurred in 10 patients (24.4%). CONCLUSIONS: Bevacizumab treatment offers certain clinical benefits for patients with surgically untreatable, symptomatic BRN. The determination of BRN using amino-acid PET, not biopsy, is adequate and less invasive for determining eligibility to receive bevacizumab.

Change in F-18-Fluoromisonidazole PET Is an Early Predictor of the Prognosis in the Patients with Recurrent High-Grade Glioma Receiving Bevacizumab Treatment
Shigeru Yamaguchi, Kenji Hirata, Takuya Toyonaga, Kentaro Kobayashi, Yukitomo Ishi, Hiroaki Motegi, Hiroyuki Kobayashi, Tohru Shiga, Nagara Tamaki, Shunsuke Terasaka, Kiyohiro Houkin
PLOS ONE, 11, 12, e0167917, Dec. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Increased Blood Viscosity in Ischemic Stroke Patients with Small Artery Occlusion Measured by an Electromagnetic Spinning Sphere Viscometer
Koji Furukawa, Takeo Abumiya, Keiji Sakai, Miki Hirano, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Kazutoshi Hida, Kiyohiro Houkin
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 25, 11, 2762, 2769, Nov. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Transarterial regional hypothermia provides robust neuroprotection in a rat model of permanent middle cerebral artery occlusion with transient collateral hypoperfusion
Kota Kurisu, Takeo Abumiya, Masaki Ito, Masayuki Gekka, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
BRAIN RESEARCH, 1651, 95, 103, Nov. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

A significant correlation between delayed cure after microvascular decompression and positive response to preoperative anticonvulsant therapy in patients with hemifacial spasm
Shunsuke Terasaka, Katsuyuki Asaoka, Shigeru Yamaguchi, Hiroyuki Kobayashi, Hiroaki Motegi, Kiyohiro Houkin
NEUROSURGICAL REVIEW, 39, 4, 607, 612, Oct. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Reliability of the Size Evaluation Method for Meningiomas: Maximum Diameter, ABC/2 Formula, and Planimetry Method
Yukitomo Ishi, Shunsuke Terasaka, Shigeru Yamaguchi, Michiharu Yoshida, Shogo Endo, Hiroyuki Kobayashi, Kiyohiro Houkin
WORLD NEUROSURGERY, 94, 80, 88, Oct. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Utility of noncontrast-enhanced time-resolved four-dimensional MR angiography with a vessel-selective technique for intracranial arteriovenous malformations
Noriyuki Fujima, Toshiya Osanai, Yukie Shimizu, Atsushi Yoshida, Taisuke Harada, Naoki Nakayama, Kohsuke Kudo, Kiyohiro Houkin, Hiroki Shirato
JOURNAL OF MAGNETIC RESONANCE IMAGING, 44, 4, 834, 845, Oct. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Cellular Functions and Gene and Protein Expression Profiles in Endothelial Cells Derived from Moyamoya Disease-Specific iPS Cells
Shuji Hamauchi, Hideo Shichinohe, Haruto Uchino, Shigeru Yamaguchi, Naoki Nakayama, Ken Kazumata, Toshiya Osanai, Takeo Abumiya, Kiyohiro Houkin, Takumi Era
PLOS ONE, 11, 9, e0163561, Sep. 2016, [Peer-reviewed]
English, Scientific journal

Measurement of human blood viscosity by an electromagnetic spinning sphere viscometer.
Koji Furukawa, Takeo Abumiya, Keiji Sakai, Miki Hirano, Toshiya Osanai, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Toshimitsu Aida, Kiyohiro Houkin
Journal of medical engineering & technology, 40, 6, 285, 92, Aug. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, We herein applied an electromagnetic spinning sphere (EMS) viscometer to the measurement of human blood viscosity for the first time. We collected blood samples from 100 healthy outpatient volunteers in order to analyse viscosity dependence on blood cell parameters and on the shear rate with a simple approximation formula [ηi (γ)\, = Ai γ(- pi) + η0]. Viscosity dependence on blood cell parameters was relatively high at a high shear rate, but became lower as the shear rate decreased. The approximation formula with appropriate parameters of Ai and pi nearly faithfully reproduced actual blood rheological behaviour with a standard deviation of 1.5%. The distributions of Ai and pi values were broad, suggesting that the pattern of viscosity dependence on the shear rate varied with individual differences. The results obtained using the EMS viscometer suggest that blood viscosity values are individual-specific and actual individual measurements are important for understanding rheological conditions.

Treatment of Recurrent Intracranial Aneurysms After Clipping: A Report of 23 Cases and a Review of the Literature
Masaaki Hokari, Ken Kazumara, Naoki Nakayama, Satoshi Ushikoshi, Taku Sugiyama, Katsunori Asaoka, Kazuki Uchida, Daisuke Shimbo, Koji Itamoto, Yuka Yokoyama, Masanori Isobe, Tetsuaki Imai, Toshiya Osanai, Kiyohiro Houkin
WORLD NEUROSURGERY, 92, 434, 444, Aug. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Transarterial Regional Brain Hypothermia Inhibits Acute Aquaporin-4 Surge and Sequential Microvascular Events in Ischemia/Reperfusion Injury
Kota Kurisu, Takeo Abumiya, Hideki Nakamura, Daisuke Shimbo, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Hiroshi Shimizu, Kiyohiro Houkin
NEUROSURGERY, 79, 1, 125, 134, Jul. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Edaravone Reduces Hyperperfusion-Related Neurological Deficits in Adult Moyamoya Disease Historical Control Study
Haruto Uchino, Naoki Nakayama, Ken Kazumata, Satoshi Kuroda, Kiyohiro Houkin
STROKE, 47, 7, 1930, 1932, Jul. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

[Efficiency of Embosphere in the Pre-Operative Embolization of Meningioma:Clinical Experience].
Yusuke Shimoda, Toshiya Osanai, Syunsuke Terasaka, Hiroyuki Kobayashi, Shigeru Yamaguchi, Shogo Endo, Kanako Hatanaka, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 44, 7, 555, 60, Jul. 2016, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, Embosphere^® was approved in Japan for embolizing intracranial arteries for the reduction of intraoperative bleeding in January 2014. Until August 2015, we performed embolization for four meningiomas using Embosphere^®. We performed an initial evaluation of all the cases, including evaluation of the clinical courses, change in the maximum tumor diameters and volumes, alteration in the appearance on magnetic resonance imaging(MRI), amount of intraoperative bleeding, complications, and histopathological findings. After embolization, the maximum tumor diameters and volumes slightly decreased on MRI, whereas the signal change on diffusion-weighted imaging(DWI)or fluid-attenuated inversion recovery(FLAIR)varied in each case. One case demonstrated a partial signal change on DWI one day after the initial procedure, and another case demonstrated a decrease in perifocal edema on FLAIR. Among our patients, least bleeding was recorded at 6 days after the embolization. Histopathological analysis revealed coagulative necrotic lesions in two cases. The von Kossa stain was used to distinguish Embosphere^® from the psammoma body. One case involved a thromboembolic complication of the retinocentral artery caused by an Embosphere^® of 100-300 μm. Our early-stage experience suggests that an Embosphere^® of 300-500 μm should be used for safe embolization before resection to avoid thromboembolic complications because an Embosphere^® of 100-300 μm can pass through the dangerous anastomosis. Our present strategy was to resect the tumor approximately seven days after the embolization using Embosphere^®. However, further studies and discussion on the size of Embosphere^®, and the interval between pre-operative embolization and surgical removal are needed.

Primary pineal rhabdomyosarcoma successfully treated by high-dose chemotherapy followed by autologous peripheral blood stem cell transplantation: case report
Yukitomo Ishi, Shigeru Yamaguchi, Akihiro Iguchi, Yuko Cho, Junjiro Ohshima, Kanako C. Hatanaka, Emi Takakuwa, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
JOURNAL OF NEUROSURGERY-PEDIATRICS, 18, 1, 41, 45, Jul. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Clinical impact of targeted amplicon sequencing for meningioma as a practical clinical-sequencing system
Sayaka Yuzawa, Hiroshi Nishihara, Shigeru Yamaguchi, Hiromi Mohri, Lei Wang, Taichi Kimura, Masumi Tsuda, Mishie Tanino, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin, Norihiro Sato, Shinya Tanaka
MODERN PATHOLOGY, 29, 7, 708, 716, Jul. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

小児脊髄係留症候群の治療成績
関 俊隆, 飛騨 一利, 矢野 俊介, 笹森 徹, 濱内 祝嗣, 寶金 清博
小児の脳神経, 41, 1, 126, 126, (一社)日本小児神経外科学会, May 2016
Japanese

Development of a nonintermediate-incision ventriculoperitoneal shunt procedure using a nasogastric feeding tube for infant patients with hydrocephalus: technical note
Shuji Hamauchi, Toshitaka Seki, Toru Sasamori, Kiyohiro Houkin
JOURNAL OF NEUROSURGERY-PEDIATRICS, 17, 5, 540, 543, May 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Familial moyamoya disease in two Turkish siblings with same polymorphism in RNF213 gene but different clinical features
Ayse Kacar Bayram, Ebru Yilmaz, Huseyin Per, Masaki Ito, Haruto Uchino, Selim Doganay, Kiyohiro Houkin, Ekrem Unal
CHILDS NERVOUS SYSTEM, 32, 3, 569, 573, Mar. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

De novo arteriovenous malformation in a patient with hereditary hemorrhagic telangiectasia.
Yusuke Shimoda, Toshiya Osanai, Naoki Nakayama, Satoshi Ushikoshi, Masaaki Hokari, Hideo Shichinohe, Takeo Abumiya, Ken Kazumata, Kiyohiro Houkin
Journal of neurosurgery. Pediatrics, 17, 3, 330, 5, Mar. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant systemic disorder characterized by the enlargement of capillaries, recurrent nosebleeds, and multiple arteriovenous malformations (AVMs). Although cerebral AVMs are traditionally considered to be congenital lesions, some reports have described de novo AVMs, which suggests that the authors believed them to be dynamic conditions. In this article, the authors describe the case of a 5-year-old boy with HHT in whom a de novo cerebral AVM was detected after a negative MRI result at 5 months. To the authors' knowledge, this is the first report of a de novo AVM in a patient with HHT. In patients with a family history of HHT, de novo AVMs are possible, even when no lesions are detected at the first screening. Therefore, regular screenings need to be performed, and the family should be informed that AVMs could still develop despite normal MRI results.

Long-term outcomes after surgical and endovascular treatment of spinal dural arteriovenous fistulae
Toru Sasamori, Kazutoshi Hida, Shunsuke Yano, Takeshi Asano, Toshitaka Seki, Kiyohiro Houkin
EUROPEAN SPINE JOURNAL, 25, 3, 748, 754, Mar. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

A Novel Large Cerebral Aneurysm Model in Rats with Intraperitoneal Administration of Beta-Amino Propio Nitril-Fumarate.
Shimoda Yusuke, Moriwaki Takuya, Nakayama Naoki, Abumiya Takeo, Kazumata Ken, Shichinohe Hideo, Houkin Kiyohiro
STROKE, 47, Feb. 2016, [Peer-reviewed]

New Clinical Application of Measurement of Blood Viscosity With an Electromagnetically Spinning Sphere Viscometer in Acute Ischemic Stroke Patients
Abumiya Takeo, Furukawa Koji, Sakai Keiji, Hirano Miki, Osanai Toshiya, Shichinohe Hideo, Nakayama Naoki, Kazumata Ken, Aida Toshimitsu, Houkin Kiyohiro
STROKE, 47, Feb. 2016, [Peer-reviewed]

The Next Generation of Autologous Bone Marrow Stromal Cell Transplantation Against Stroke
Shichinohe Hideo, Tan Chengbo, Saito Hisayasu, Miyamoto Michiyuki, Hamauchi Shuji, Abumiya Takeo, Nakayama Naoki, Kazumata Ken, Houkin Kiyohiro, Kuroda Satoshi
STROKE, 47, Feb. 2016, [Peer-reviewed]

Trans-arterial Regional Hypothermia Provides Robust Neuroprotection in Rat Permanent Middle Cerebral Artery Occlusion Model
Kurisu Kota, Abumiya Takeo, Ito Masaki, Shichinohe Hideo, Nakayama Naoki, Kazumata Ken, Osanai Toshiya, Houkin Kiyohiro
STROKE, 47, Feb. 2016, [Peer-reviewed]

Effects of Surgery and Antiplatelet Therapy in Ten-Year Follow-Up from the Registry Study of Research Committee on Moyamoya Disease in Japan
Satoshi Yamada, Koichi Oki, Yoshiaki Itoh, Satoshi Kuroda, Kiyohiro Houkin, Teiji Tominaga, Susumu Miyamoto, Nobuo Hashimoto, Norihiro Suzuki
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 25, 2, 340, 349, Feb. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Successful Acute Endovascular Therapy of Cerebral Embolism for a Patient with Ventricle-assist Device: Case Report
Kobayashi Satoshi, Osanai Toshiya, Miyamoto Michiyuki, Shimoda Yusuke, Ushikochi Satoshi, Ooka Tomonori, Matsui Yoshiro, Hatanaka Kanako, Houkin Kiyohiro
Journal of Neuroendovascular Therapy, The Japanese Society for Neuroendovascular Therapy, 2016
English, Objective: Ventricular assist devices (VAD) are used as a bridge to heart transplantation. Cerebral embolism can develop in patients with VADs despite a strict anticoagulation therapy. We report two cases of successful acute endovascular therapy with Penumbra system for cerebral embolism.Case Presentations: Case 1: A 20-year-old woman with a VAD presented with right facial palsy and right monoparesis. 3D-computed tomography angiography (3D-CTA) demonstrated left middle cerebral artery (MCA) occlusion. We successfully performed endovascular recanalization of the left MCA with Thrombolysis in Cerebral Infarction (TICI) Grade3 perfusion, and the patient's symptoms significantly improved. The patient's condition improved to nearly the pre-treatment state. However, due to repeated strokes, the patient developed disturbances of consciousness, aphasia, and complete paralysis of the left lower limb. Furthermore, the infection worsened, and the patient eventually developed a circulatory disorder of the VAD and died. Case 2: A 34-year-old man with VAD presented with sudden disturbances of consciousness and right hemiparesis. Computed tomography (CT) scan revealed no early abnormalities. We performed endovascular recanalization of the left internal carotid artery (IC) top with TICI 3 perfusion. After endovascular treatment, CT scan did not reveal a cerebral infarction. The patient eventually achieved an National Institutes of Health Stroke Scale (NIHSS) score of 0 point and received a heart transplant. In case 2, we pathologically analyzed the retrieved thrombus and confirmed the thrombus to be mainly composed of fibrin. There were no complications of hemorrhage in either case.Conclusion: A suction-type thrombectomy device is believed to be the first choice for patients with VAD.

脳梗塞に対する自家骨髄間質細胞移植
七戸秀夫, 寳金清博
脳循環代謝(Web), 28, 1, 2016

脳梗塞の細胞治療と『革新的医薬品・医療機器・再生医療製品実用化促進事業』
寳金清博
レギュラトリーサイエンス学会誌, 6, Supplement, 2016

Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
Chengbo Tan, Hideo Shichinohe, Zifeng Wang, Shuji Hamauchi, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Tsuneo Ito, Kohsuke Kudo, Shigeru Takamoto, Kiyohiro Houkin
Stem Cells International, 2016, Hindawi Limited, 2016, [Peer-reviewed]
English, Scientific journal

Postoperative Acute Submandibular Sialadenitis after Neurosurgery: Two Case Reports and a Review of the Literature.
Uchino H, Motegi H, Kobayashi H, Kamoshima Y, Kazumata K, Terasaka S, Houkin K
NMC case report journal, 3, 1, 1, 4, Jan. 2016, [Peer-reviewed], [Domestic magazines]
English, This article details two case reports and reviews the extant literature concerning acute postoperative submandibular sialadenitis occurring in the side contralateral to the operated site after neurosurgery. Although its precise pathogenesis remains to be elucidated, it is likely related to intraoperative compression of the submandibular gland and surrounding tissues, caused by head positioning and the endotracheal tube. Submandibular swelling rapidly deteriorates after surgery, and emergent airway protection is required in most cases in order to avoid fatal airway obstruction. To avoid serious sequelae, we should be aware of acute submandibular sialadenitis that occurs contralateral to the surgical side, which, although rare, is more probable after posterior fossa surgery. When it occurs, early airway protection is crucial, and the following conservative treatment could provide a good prognosis.

In Reply: A Survey of Chronic Pain Due to Spinal Dural Arteriovenous Fistulae
Toru Sasamori, Kazutoshi Hida, Toshiya Osanai, Shunsuke Yano, Toshitaka Seki, Kiyohiro Houkin
NEUROSURGERY, 78, 1, E162, E162, Jan. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Application of endoscopic ultrasonography to intraventricular lesions
Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Shigeru Yamaguchi, Yukitomo Ishi, Yasuhiro Ito, Kiyohiro Houkin
ACTA NEUROCHIRURGICA, 158, 1, 87, 92, Jan. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Ultrasound-guided Femoral Artery Access for Minimally Invasive Neuro-intervention and Risk Factors for Access Site Hematoma
Kota Kurisu, Toshiya Osanai, Ken Kazumata, Naoki Nakayama, Takeo Abumiya, Hideo Shichinohe, Yusuke Shimoda, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 56, 12, 745, 752, 2016, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Cell Therapy for Stroke: Review of Previous Clinical Trials and Introduction of Our New Trials
Hideo Shichinohe, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 56, 10, 592, 596, 2016, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Clinical factors for prognosis and treatment guidance of spinal cord astrocytoma
Toshitaka Seki, Kazutoshi Hida, Shunsuke Yano, Takeshi Aoyama, Izumi Koyanagi, Toru Sasamori, Shuji Hamauch, Kiyohiro Houkin
Asian Spine Journal, 10, 4, 748, 754, Korean Society of Spine Surgery, 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Lateral position of the external carotid artery: A rare variation to be recognized during carotid endarterectomy
Masaki Ito, Yoshimasa Niiya, Masashi Kojima, Hiroyuki Itosaka, Motoyuki Iwasaki, Ken Kazumata, Shoji Mabuchi, Kiyohiro Houkin
Acta Neurochirurgica, Supplementum, 123, 115, 122, Springer-Verlag Wien, 2016, [Peer-reviewed], [International Magazine]
English, In book

Endoscopic transsphenoidal surgery using pedicle vascularized nasoseptal flap for cholesterol granuloma in petrous apex: A technical note
Yukitomo Ishi, Hiroyuki Kobayashi, Hiroaki Motegi, Shogo Endo, Shigeru Yamaguchi, Shunsuke Terasaka, Kiyohiro Houkin
NEUROLOGIA I NEUROCHIRURGIA POLSKA, 50, 6, 504, 510, 2016, [Peer-reviewed]
English, Scientific journal

Surgical outcome of children and adolescents with tethered cord syndrome
Toshitaka Seki, Kazutoshi Hida, Shunsuke Yano, Toru Sasamori, Shuji Hamauch, Izumi Koyanagi, Kiyohiro Houkin
Asian Spine Journal, 10, 5, 940, 944, Korean Society of Spine Surgery, 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
Chengbo Tan, Hideo Shichinohe, Zifeng Wang, Shuji Hamauchi, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Tsuneo Ito, Kohsuke Kudo, Shigeru Takamoto, Kiyohiro Houkin
STEM CELLS INTERNATIONAL, 2016, 6104780, 6104780, 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Significance of the Hemorrhagic Site for Recurrent Bleeding Prespecified Analysis in the Japan Adult Moyamoya Trial
Jun C. Takahashi, Takeshi Funaki, Kiyohiro Houkin, Tooru Inoue, Kuniaki Ogasawara, Jyoji Nakagawara, Satoshi Kuroda, Keisuke Yamada, Susumu Miyamoto
STROKE, 47, 1, 37, 43, Jan. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

[Efficacy of Stent-Assisted Coil Embolization for a Dissecting Aneurysm of the Cervical Internal Carotid Artery Caused by a Systemic Vascular Disease: A Case Report].
Soichiro Takamiya, Toshiya Osanai, Satoshi Ushikoshi, Kota Kurisu, Yusuke Shimoda, Yasuhiro Ito, Yukitomo Ishi, Masaaki Hokari, Naoki Nakayama, Ken Kazumata, Takeo Abumiya, Hideo Shichinohe, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 44, 1, 39, 45, Jan. 2016, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, Systemic vascular diseases such as fibromuscular dysplasia, Ehlers-Danlos syndrome, Marfan syndrome, and Behçet's disease are known to cause spontaneous dissecting aneurysms of the cervical internal carotid artery. These diseases are generally associated with vascular fragility; therefore, invasive treatments are avoided in many cases of dissecting aneurysms, and a conservative approach is used for the primary disease. Surgical or intravascular treatment may be chosen when aneurysms are progressive or are associated with a high risk of hemorrhage; however, there is no consensus on which treatment is better. We report a case of a dissecting aneurysm of the cervical internal carotid artery in a patient with suspected Behçet's disease, which was treated using stent-assisted coil embolization. A man in his 40's, with suspected Behçet's disease, presented with an enlarged dissecting aneurysm of the right cervical internal carotid artery. The lesion was present for approximately 10 years. We performed stent-assisted coil embolization for the lesion. Post-surgery, no aneurysms were detected with carotid artery echography. Our case report suggests that stent-assisted coil embolization is a promising treatment for dissecting aneurysms of the cervical internal carotid artery. In addition, the procedure demonstrates the utility of carotid artery echograms for examining recanalization after stent-assisted coil embolization.

Reappraisal of Microsurgical Revascularization for Anterior Circulation Ischemia in Patients with Progressive Stroke.
Taku Sugiyama, Ken Kazumata, Katsuyuki Asaoka, Toshiya Osanai, Daisuke Shimbo, Kazuki Uchida, Yuka Yokoyama, Naoki Nakayama, Kouji Itamoto, Kiyohiro Houkin
World neurosurgery, 84, 6, 1579, 88, Dec. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Intravenous thrombolysis using tissue plasminogen activator and endovascular treatment for acute ischemic stroke is becoming an established standard therapy. However, there is no consensus in the treatment of patients who are suffering from progressive neurologic symptoms in the later stages. The purpose of this study was to evaluate the safety and efficacy of microsurgical revascularization in such patients with progressive stroke. METHODS: We retrospectively reviewed the clinical and radiological records of 14 consecutive patients with progressive stroke who underwent emergency open surgery for anterior circulation occlusion within 7 days after onset. Surgical candidates were carefully selected on the basis of symptom severity, diffusion-weighted imaging, and perfusion study. Superficial temporal artery to middle cerebral artery bypass was applied for atherosclerotic occlusion, and microsurgical embolectomy was applied for embolic occlusion. RESULTS: Superficial temporal artery to middle cerebral artery bypass was performed in 12 patients, microsurgical embolectomy in 1, and the combination of these modalities in 1. As a result, complete revascularization was achieved in all patients. The National Institutes of Health Stroke Scale scores significantly improved after surgery (at third postoperative day, P < 0.05; at 14th postoperative day, P < 0.01). A favorable outcome (modified Rankin Scale 0-2) was achieved in 12 of the 14 (85.7%) patients. Minor intracerebral hemorrhage occurred in 1 patient and hyperperfusion syndrome occurred in 1 patients; however, the patients subsequently recovered without additional treatment. CONCLUSIONS: Microsurgical revascularization is a feasible treatment option for patients with progressive stroke due to anterior circulation major vessel occlusion.

脊髄硬膜動静脈瘻に対する血管内治療の現状と塞栓術成功因子の検討
栗栖 宏多, 長内 俊也, 飛彈 一利, 浅野 剛, 牛越 聡, 関 俊隆, 笹森 徹, 寶金 清博
JNET: Journal of Neuroendovascular Therapy, 9, 6, S316, S316, (NPO)日本脳神経血管内治療学会, Nov. 2015
Japanese

Risk of rupture of unruptured cerebral aneurysms in elderly patients
Tomohito Hishikawa, Isao Date, Koji Tokunaga, Shinjiro Tominari, Kazuhiko Nozaki, Yoshiaki Shiokawa, Kiyohiro Houkin, Yuichi Murayama, Toshihiro Ishibashi, Hiroyuki Takao, Toshikazu Kimura, Takeo Nakayama, Akio Morita
NEUROLOGY, 85, 21, 1879, 1885, Nov. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

キャンサーボードが機能した1症例　　　　　　　　　　　　　　　
遠藤 将吾, 寺坂 俊介, 小林 浩之, 山口 秀, 茂木 洋晃, 金子 貞洋, 井口 晶裕, 長 祐子, 大島 淳二郎, 寺坂 友佳代, 杉山 未奈子, 橋本 孝之, 鬼丸 力也, 清水 伸一, 有賀 正, 白戸 博樹, 寳金 清博
日本小児血液・がん学会雑誌, 52, 3, 343, 343, (NPO)日本小児血液・がん学会, Oct. 2015
Japanese

A novel application of four-dimensional magnetic resonance angiography using an arterial spin labeling technique for noninvasive diagnosis of Moyamoya disease
Haruto Uchino, Masaki Ito, Noriyuki Fujima, Ken Kazumata, Kazuyoshi Yamazaki, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
CLINICAL NEUROLOGY AND NEUROSURGERY, 137, 105, 111, Oct. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Ceacam1L Modulates STAT3 Signaling to Control the Proliferation of Glioblastoma-Initiating Cells
Sadahiro Kaneko, Yuka Nakatani, Tatsuya Takezaki, Takuichiro Hide, Daisuke Yamashita, Naoki Ohtsu, Takanori Ohnishi, Shunsuke Terasaka, Kiyohiro Houkin, Toru Kondo
CANCER RESEARCH, 75, 19, 4224, 4234, Oct. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Cerebral aneurysms associated with segmental dilative arteriopathy of the circle of Willis
Yoshifumi Horita, Takeshi Mikami, Kiyohiro Houkin, Nobuhiro Mikuni
Surgical Neurology International, 6, Suppl 9, S291, S294, Medknow Publications, 01 Sep. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

PORPHYRIN DERIVATIVES-MEDIATED SONODYNAMIC THERAPY FOR MALIGNANT GLIOMAS IN VITRO
Shogo Endo, Nobuki Kudo, Shigeru Yamaguchi, Koki Sumiyoshi, Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
ULTRASOUND IN MEDICINE AND BIOLOGY, 41, 9, 2458, 2465, Sep. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Inside Intramural Thrombus Formation with Inflammatory Reactions is Relevant to the Rupture of Cerebral Aneurysms
Shimoda Yusuke, Nakayama Naoki, Hokari Masaaki, Abumiya Takeo, Shichinohe Hideo, Kazumata Ken, Houkin Kiyohiro
JOURNAL OF NEUROSURGERY, 123, 2, A526, Aug. 2015, [Peer-reviewed]

Surgical Outcomes for Cervical Carotid Artery Stenosis: Treatment Strategy for Bilateral Cervical Carotid Artery Stenosis.
Masaaki Hokari, Naoki Nakayama, Ken Kazumata, Toshiya Osanai, Toshitaka Nakamura, Hiroshi Yasuda, Satoshi Ushikoshi, Hideo Shichinohe, Takeo Abumiya, Satoshi Kuroda, Kiyohiro Houkin
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 24, 8, 1768, 74, Aug. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Carotid endarterectomy (CEA) and carotid stenting (CAS) are beneficial procedures for patients with high-grade cervical carotid stenosis. However, it is sometimes difficult to manage patients with bilateral carotid stenosis. To decide the treatment strategy, one of the most important questions is whether contralateral stenosis increases the risk of patients undergoing CEA. METHODS: This retrospective study included 201 patients with carotid stenosis who underwent a total of 219 consecutive procedures (CEA 189/CAS 30). We retrospectively analyzed outcomes in patients with carotid stenosis who were treated with either CEA or CAS and evaluated whether or not contralateral lesions increases the risk of patients undergoing CEA or CAS. Furthermore, we retrospectively verified our treatment strategy for bilateral carotid stenosis. RESULTS: The incidences of perioperative complications were 5.3% in the CEA patients and 6.7% in the CAS patients, respectively. There was no significant difference between these 2 groups. The existences of contralateral occlusion and/or contralateral stenosis were not associated with perioperative complications in both the groups. There were 32 patients with bilateral severe carotid stenosis (>50%). Of those, 13 patients underwent bilateral revascularizations; CEA followed by CEA in 8, CEA followed by CAS in 3, CAS followed by CEA + coronary artery bpass grafting in 1, and CAS followed by CAS in 1. CONCLUSIONS: Our date showed that the existence of contralateral carotid lesion was not associated with perioperative complications, and most of our cases with bilateral carotid stenosis initially underwent CEA.

Risk factors for adverse events after implantation of BCNU wafers in high-grade gliomas
Michiharu Yoshida, Shigeru Yamaguchi, Yukitomo Ishi, Shogo Endo, Hiroaki Motegi, Hiroyuki Kobayashi, Katsuyuki Asaoka, Yuuta Kamoshima, Shunsuke Terasaka, Kiyohiro Houkin
Neurological Surgery, 43, 7, 603, 610, Igaku-Shoin Ltd, 01 Jul. 2015, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

A Survey of Chronic Pain Due to Spinal Dural Arteriovenous Fistulae.
Toru Sasamori, Kazutoshi Hida, Toshiya Osanai, Shunsuke Yano, Toshitaka Seki, Kiyohiro Houkin
Neurosurgery, 77, 1, 113, 8, Jul. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Chronic pain due to spinal dural arteriovenous fistulae (SDAVF) during follow-up is a serious issue because it can affect patients' quality of life. The severity of posttreatment chronic leg pain in patients with SDAVF is unclear. OBJECTIVE: To investigate the prevalence, severity, and characteristics of chronic leg pain in patients with SDAVF and to examine the clinical factors associated with chronic pain. METHODS: We conducted a survey of their chronic posttreatment leg pain in 49 SDAVF patients. It consisted of a numeric rating scale and a neuropathic pain symptom inventory (NPSI). Of the 49 patients, 32 (65.3%) completed the questionnaire. There were 24 males and 8 females whose mean age was 69.1 years. The mean follow-up period was 86.3 months. RESULTS: Chronic leg pain was reported by 26 (81.3%) of 32 patients; 14 experienced pain before treatment and 12 had new-onset posttreatment pain. The pain was reported as moderate to severe by 21 (80.8%) of 26 patients; the mean numeric rating scale and NPSI scores were 6.0 ± 2.3 and 17.4 ± 10.3, respectively. The NPSI subscores were significantly higher for spontaneous pain and paresthesia/dysesthesia than for paroxysmal pain. We found no significant clinical factors related to chronic posttreatment pain; on magnetic resonance imaging scans we identified spinal cord atrophy in 8 patients with chronic pain. CONCLUSION: Most of our SDAVF patients reported moderate to severe chronic leg pain characterized by spontaneous pain and paresthesia/dysesthesia. Spinal cord atrophy on magnetic resonance imaging scans was a characteristic in patients with chronic pain.

Pathological findings of saccular cerebral aneurysms-impact of subintimal fibrin deposition on aneurysm rupture
Masaaki Hokari, Naoki Nakayama, Hiroshi Nishihara, Kiyohiro Houkin
NEUROSURGICAL REVIEW, 38, 3, 531, 540, Jul. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Prediction model for 3-year rupture risk of unruptured cerebral aneurysms in Japanese patients
Shinjiro Tominari, Akio Morita, Toshihiro Ishibashi, Tomosato Yamazaki, Hiroyuki Takao, Yuichi Murayama, Makoto Sonobe, Masahiro Yonekura, Nobuhito Saito, Yoshiaki Shiokawa, Isao Date, Teiji Tominaga, Kazuhiko Nozaki, Kiyohiro Houkin, Susumu Miyamoto, Takaaki Kirino, Kazuo Hashi, Takeo Nakayama
ANNALS OF NEUROLOGY, 77, 6, 1050, 1059, Jun. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Short-, middle- and long-term safety of superparamagnetic iron oxide-labeled allogeneic bone marrow stromal cell transplantation in rat model of lacunar infarction
Chengbo Tan, Hideo Shichinohe, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Masaaki Hokari, Shuji Hamauchi, Kiyohiro Houkin
NEUROPATHOLOGY, 35, 3, 197, 208, Jun. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

脊髄動静脈瘻手術における術中MEPモニタリングの有用性
山本 雅史, 関 俊隆, 笹森 徹, 飛騨 一利, 奥原 浩之, 村上 望, 田中 小枝子, 川口 朋香, 中根 進児, 渋谷 斉, 重松 明男, 清水 力, 寶金 清博
脊髄外科, 29, 1, 42, 46, (一社)日本脊髄外科学会, Apr. 2015
Japanese

Neuroprotective effects of cilostazol are mediated by multiple mechanisms in a mouse model of permanent focal ischemia
Hideo Shichinohe, Chengbo Tan, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Masaaki Hokari, Kiyohiro Houkin, Satoshi Kuroda
BRAIN RESEARCH, 1602, 53, 61, Mar. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Therapeutic Effects of Human Multilineage-Differentiating Stress Enduring (MUSE) Cell Transplantation into Infarct Brain of Mice
Tomohiro Yamauchi, Yasumasa Kuroda, Takahiro Morita, Hideo Shichinohe, Kiyohiro Houkin, Mari Dezawa, Satoshi Kuroda
PLOS ONE, 10, 3, e0116009, Mar. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Cognitive dysfunction survey of the japanese patients with moyamoya disease (Cosmo-japan study): Study protocol
Yasushi Takagi, Susumu Miyamoto, COSMO-Japan Study Group, Kiyohiro Houkin, Joji Nakagawara, Kuniaki Ogasawara, Teiji Tominaga, Yoshikazu Okada, Tadashi Nariai, Satoshi Kuroda, Yukihiko Fujii, Toshihiko Wakabayashi, Kazuo Yamada, Susumu Miyamoto, Hiroyuki Nakase, Koji Iihara, Toshio Matsushima, Izumi Nagata, Toshiya Murai, Tomohisa Okada, Yasushi Takagi
Neurologia Medico-Chirurgica, 55, 3, 199, 203, Japan Neurosurgical Society, 20 Feb. 2015, [Peer-reviewed]
English, Scientific journal

骨転移をきたした小脳膠芽腫の1例　　　　　　　　　　　　　　　
湊川 英樹, 森 崇, 鬼丸 力也, 小野寺 俊輔, 白土 博樹, 東海林 菊太郎, 吉田 道春, 小林 浩之, 寳金 清博, 菅野 宏美, 田中 伸哉
Japanese Journal of Radiology, 33, Suppl., 4, 4, (公社)日本医学放射線学会, Feb. 2015
Japanese

Prediction of Postoperative Hyperperfusion by Intraoperative Assessment of Indocyanine Green Videoangiography in Moyamoya Disease
Uchino Haruto, Ito Masaki, Kazumata Ken, Nakayama Naoki, Kuroda Satoshi, Houkin Kiyohiro
STROKE, 46, Feb. 2015, [Peer-reviewed]

Cilostazol Can Attenuate Oxidative Stress by Suppressing Nox2 on Neurons in Mice Permanent Focal Ischemia
Shichinohe Hideo, Kuroda Satoshi, Tan Chengbo, Abumiya Takeo, Nakayama Naoki, Kazumata Ken, Hokari Masaaki, Houkin Kiyohiro
STROKE, 46, Feb. 2015, [Peer-reviewed]

Inside Intramural Thrombus Formation with Inflammatory Reactions Is Relevant to the Rupture of Cerebral Aneurysms
Shimoda Yusuke, Nakayama Naoki, Hokari Masaaki, Abumiya Takeo, Shichinohe Hideo, Kazumata Ken, Houkin Kiyohiro
STROKE, 46, Feb. 2015, [Peer-reviewed]

Post-ischemic Trans-arterial Regional Hypothermia Therapy Attenuates Microvascular Narrowing due to Astrocyte End-feet Swelling and Inhibits Subsequent Inflammatory Reactions in Ischemia Reperfusion Injury
Kurisu Kota, Abumiya Takeo, Nakamura Hideki, Shimbo Daisuke, Kazumata Ken, Nakayama Naoki, Shichinohe Hideo, Hokari Masaaki, Osanai Toshiya, Shimizu Hiroshi, Houkin Kiyohiro
STROKE, 46, Feb. 2015, [Peer-reviewed]

Utility of 4D-MRA to Measure Blood Flow Velocity in Cerebral Major Arteries
Abumiya Takeo, Fujima Noriyuki, Kudo Kohsuke, Ishi Yukitomo, Gekka Masayuki, Shichinohe Hideo, Kazumata Ken, Nakayama Naoki, Houkin Kiyohiro
STROKE, 46, Feb. 2015, [Peer-reviewed]

Combined use of 18 F-FDG PET and corticosteroid for diagnosis of deep-seated primary central nervous system lymphoma without histopathological confirmation
Shigeru Yamaguchi, Kenji Hirata, Sadahiro Kaneko, Hiroyuki Kobayashi, Tohru Shiga, Kentaro Kobayashi, Rikiya Onimaru, Hiroki Shirato, Nagara Tamaki, Shunsuke Terasaka, Kiyohiro Houkin
ACTA NEUROCHIRURGICA, 157, 2, 187, 194, Feb. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Chronic Ischemia Alters Brain Microstructural Integrity and Cognitive Performance in Adult Moyamoya Disease
Ken Kazumata, Khin Khin Tha, Hisashi Narita, Ichiro Kusumi, Hideo Shichinohe, Masaki Ito, Naoki Nakayama, Kiyohiro Houkin
STROKE, 46, 2, 354, 360, Feb. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Comparison of postoperative volume status and hemodynamics between surgical clipping and endovascular coiling in patients after subarachnoid hemorrhage
Tatsushi Mutoh, Ken Kazumata, Yuka Yokoyama, Tatsuya Ishikawa, Yasuyuki Taki, Shunsuke Terasaka, Kiyohiro Houkin
Journal of Neurosurgical Anesthesiology, 27, 1, 7, 15, Lippincott Williams and Wilkins, 12 Jan. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Bone Marrow Stromal Cells Rescue Ischemic Brain by Trophic Effects and Phenotypic Change Toward Neural Cells
Hideo Shichinohe, Takeshi Ishihara, Koji Takahashi, Yoshikazu Tanaka, Michiyuki Miyamoto, Tomohiro Yamauchi, Hisayasu Saito, Hiroshi Takemoto, Kiyohiro Houkin, Satoshi Kuroda
NEUROREHABILITATION AND NEURAL REPAIR, 29, 1, 80, 89, Jan. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

キャンサーボードが機能した1症例
遠藤将吾, 寺坂俊介, 小林浩之, 山口秀, 茂木洋晃, 金子貞洋, 井口晶裕, 長祐子, 大島淳二郎, 寺坂友佳代, 杉山未奈子, 橋本孝之, 鬼丸力也, 清水伸一, 有賀正, 白戸博樹, 寳金清博
日本小児血液・がん学会雑誌(Web), 52, 3, 2015

Surgical outcomes of high-grade spinal cord gliomas
Toshitaka Seki, Kazutoshi Hida, Syunsuke Yano, Takeshi Aoyama, Izumi Koyanagi, Kiyohiro Houkin
Asian Spine Journal, 9, 6, 935, 941, Korean Society of Spine Surgery, 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Unilateral Moyamoya Phenomenon with a String-of-beads Appearance in an Elderly Patient with the c.14576G > A Heterozygous Variant of RNF213
Junya Aoki, Kensaku Shibazaki, Masaki Ito, Naoki Saji, Junichi Uemura, Kiyohiro Houkin, Kazumi Kimura
INTERNAL MEDICINE, 54, 8, 971, 974, 2015, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Cerebral aneurysms associated with segmental dilative arteriopathy of the circle of Willis.
Horita Y, Mikami T, Houkin K, Mikuni N
Surgical neurology international, 6, Suppl 9, S291, 4, 2015, [Peer-reviewed]

Predictive Factors for Epilepsy in Moyamoya Disease
Takeshi Mikami, Satoko Ochi, Kiyohiro Houkin, Yukinori Akiyama, Masahiko Wanibuchi, Nobuhiro Mikuni
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 24, 1, 17, 23, Jan. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Platelet Lysate and Granulocyte-Colony Stimulating Factor Serve Safe and Accelerated Expansion of Human Bone Marrow Stromal Cells for Stroke Therapy
Tomohiro Yamauchi, Hisayasu Saito, Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Satoshi Kuroda
TRANSLATIONAL STROKE RESEARCH, 5, 6, 701, 710, Dec. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Successful acute endovascular therapy of cerebral embolism for a patient with ventricular assist device: A case report
Satoshi Kobayashi, Michiyuki Miyamoto, Shinichiro Shinada, Yukitomo Ishi, Yusuke Shimoda, Kazuyoshi Yamazaki, Satoshi Ushikoshi, Tomonori Ooka, Yoshiro Matsui, Kiyohiro Houkin
Neurological Surgery, 42, 11, 1057, 1062, Igaku-Shoin Ltd, 01 Nov. 2014, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Surgical Management of Combined Intramedullary Arteriovenous Malformation and Perimedullary Arteriovenous Fistula within the Hybrid Operating Room after Five Years of Performing Focus Fractionated Radiotherapy: Case Report
Masayuki Gekka, Toshitaka Seki, Kazutoshi Hida, Toshiya Osanai, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 54, 11, 936, 940, Nov. 2014, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Pathological Considerations for Unruptured Dissecting Aneurysm in the Posterior Inferior Cerebellar Artery: Case Report.
Miyamoto M, Nakayama N, Hokari M, Kuroda S, Takikawa S, Houkin K
NMC case report journal, 1, 1, 9, 11, Oct. 2014, [Peer-reviewed], [Domestic magazines]
English, Because of the wide spread of magnetic resonance image (MRI), it may be increasing to find a dissecting aneurysm (DA) of the posterior inferior cerebellar artery (PICA) that causes headache without associated hemorrhage or infarction. Generally, surgical treatment might be considered in cases of DA with hemorrhage. However, the treatment of an unruptured DA with headache or infarction has not been well established. This is the first report regarding the pathology of an intact DA of the PICA that presents with headache only, and the pathological findings showed interesting figures. A 44-year-old man with an unruptured DA of the left PICA presented with sudden left occipital headache. MRI revealed no hemorrhage or infarction. Magnetic resonance angiography (MRA) showed growth of the DA 12 days after the onset of headache. Therefore, surgery was performed to prevent aneurysmal rupture. This DA aneurysm was trapped and removed after an occipital artery (OA)-PICA anastomosis was performed. The surgery was performed without complication. Pathological findings showed folding of the internal elastic lamina, and the true lumen was torn by the false lumen. The dissection reached the adventitia and the wall had numerous macrophages. Pathological findings might help understanding the etiology of DAs and inflammation might play an important role in DAs.

Intraoperative assessment of cortical perfusion by indocyanine green videoangiography in surgical revascularization for moyamoya disease
Haruto Uchino, Ken Kazumata, Masaki Ito, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
ACTA NEUROCHIRURGICA, 156, 9, 1753, 1760, Sep. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effects of Surgical Revascularization on Cerebral Oxygen Metabolism in Patients With Moyamoya Disease An O-15-Gas Positron Emission Tomographic Study
Satoshi Kuroda, Daina Kashiwazaki, Kenji Hirata, Tohru Shiga, Kiyohiro Houkin, Nagara Tamaki
STROKE, 45, 9, 2717, 2721, Sep. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

The frequency of postoperative stroke in moyamoya disease following combined revascularization: a single-university series and systematic review
Ken Kazumata, Masaki Ito, Kikutaro Tokairin, Yasuhiro Ito, Kiyohiro Houkin, Naoki Nakayama, Satoshi Kuroda, Tatsuya Ishikawa, Hiroyasu Kamiyama
JOURNAL OF NEUROSURGERY, 121, 2, 432, 440, Aug. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells
Hiroaki Motegi, Yuuta Kamoshima, Shunsuke Terasaka, Hiroyuki Kobayashi, Kiyohiro Houkin
NEUROPATHOLOGY, 34, 4, 378, 385, Aug. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

The diagnostic role of F-18-FDG PET for primary central nervous system lymphoma
Shigeru Yamaguchi, Kenji Hirata, Hiroyuki Kobayashi, Tohru Shiga, Osamu Manabe, Kentaro Kobayashi, Hiroaki Motegi, Shunsuke Terasaka, Kiyohiro Houkin
ANNALS OF NUCLEAR MEDICINE, 28, 7, 603, 609, Aug. 2014, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Impact of cervical internal carotid clamping and radial artery graft bypass on cortical arterial perfusion pressure during craniotomy
Ken Kazumata, Hiroyasu Kamiyama, Tatsuya Ishikawa, Toshitaka Nakamura, Shunsuke Terasaka, Kiyohiro Houkin
NEUROSURGICAL REVIEW, 37, 3, 493, 500, Jul. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Thalamic arteriovenous malformation fed by the artery of Percheron originating from the contralateral posterior cerebral artery in a child
Hiroaki Motegi, Shunsuke Terasaka, Hideaki Shiraishi, Kiyohiro Houkin
CHILDS NERVOUS SYSTEM, 30, 7, 1313, 1315, Jul. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Spatial Relationship between Cerebral Microbleeds, Moyamoya Vessels, and Hematoma in Moyamoya Disease
Ken Kazumata, Daisuke Shinbo, Masaki Ito, Hideo Shichinohe, Satoshi Kuroda, Naoki Nakayama, Kiyohiro Houkin
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 23, 6, 1421, 1428, Jul. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Irregular Neointimal Lining with Prominent Proliferative Activity After Carotid Patch Angioplasty: An Autopsy Case Report
Masaki Ito, Yoshimasa Niiya, Haruto Uchino, Naoki Nakayama, Shoji Mabuchi, Kiyohiro Houkin
WORLD NEUROSURGERY, 82, 1-2, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Prognostic Factors for Survival in Patients with High-Grade Meningioma and Recurrence-Risk Stratification for Application of Radiotherapy
Shigeru Yamaguchi, Shunsuke Terasaka, Hiroyuki Kobayashi, Katsuyuki Asaoka, Hiroaki Motegi, Hiroshi Nishihara, Hiromi Kanno, Rikiya Onimaru, Yoichi M. Ito, Hiroki Shirato, Kiyohiro Houkin
PLOS ONE, 9, 5, e97108, May 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Utility of Early Post-treatment Single-Photon Emission Computed Tomography Imaging to Predict Outcome in Stroke Patients Treated with Intravenous Tissue Plasminogen Activator
Takeo Abumiya, Masahito Katoh, Takuya Moriwaki, Masami Yoshino, Takeshi Aoki, Hiroyuki Imamura, Toshimitsu Aida, Hideo Scichinohe, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 23, 5, 896, 901, May 2014, [Peer-reviewed]
English, Scientific journal

Clinicopathologic Study of Pineal Parenchymal Tumors of Intermediate Differentiation
Tamio Ito, Hiromi Kanno, Ken-ichi Sato, Mitsuteru Oikawa, Yoshimaru Ozaki, Hirohiko Nakamura, Shunsuke Terasaka, Hiroyuki Kobayashi, Kiyohiro Houkin, Kanako Hatanaka, Jyun-ichi Murata, Shinya Tanaka
WORLD NEUROSURGERY, 81, 5-6, 783, 789, May 2014, [Peer-reviewed]
English, Scientific journal

Regression of dural arteriovenous fistulae after venous flow reconstructive surgery in a case with hemangiopericytoma at the confluence of sinuses.
Kota Kurisu, Hiroaki Motegi, Toshiya Osanai, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin
Case reports in neurology, 6, 2, 207, 12, 2, May 2014, [Peer-reviewed], [International Magazine]
English, BACKGROUND: The mechanism by which acquired dural arteriovenous fistula (dAVF) develops is still unclear. Few cases have been reported with both dAVF and intracranial tumors, and in these few cases the authors have proposed that induced venous hypertension may lead to the pathogenesis of dAVF. We experienced a case of intrasinusoidal hemangiopericytoma (HPC) with dAVF development. In addition to its rare pathology and tumor location, this case showed regression of dAVF immediately after tumor removal. CASE REPORT: The patient was a 23-year-old man who developed progressively worse headaches and papilledema. The HPC was located entirely inside the confluence of the sinuses (CoS) and resulted in venous sinus occlusion. Cerebral angiography demonstrated a dAVF located in the straight sinus, upstream of the occluded CoS, which was fed by the dural branch of the posterior cerebral artery. After the endovascular embolization of the tumor feeders, subsequent surgery included venous reconstruction in addition to tumor excision. Although the dAVF was not treated with an endovascular procedure or surgery, postoperative angiography revealed complete disappearance of the dAVF. CONCLUSION: We conclude that venous reconstructive surgery greatly contributed to the immediate regression of the dAVF. When planning the treatment strategy for such cases, it should be remembered that acquired dAVF may regress due to the normalization of venous hypertension.

Successful removal of a metastatic gastrointestinal stromal tumor in the craniovertebral junction using an occipital artery to posterior inferior cerebellar artery bypass
Yukitomo Ishi, Naoki Nakayama, Hiroyuki Kobayashi, Shigeru Yamaguchi, Shunsuke Terasaka, Kiyohiro Houkin
Case Reports in Neurology, 6, 2, 139, 143, S. Karger AG, 16 Apr. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

A case of cervical juxtafacet cyst with extensive rim enhancement on Gd-DTPA MRI
Toru Sasamori, Kazutoshi Hida, Kimio Anzai, Shunsuke Yano, Yasutaka Kato, Shinya Tanaka, Hisatoshi Saito, Kiyohiro Houkin
CLINICAL IMAGING, 38, 2, 199, 201, Mar. 2014, [Peer-reviewed]
English, Scientific journal

Post-ischemic intra-arterial infusion of liposome-encapsulated hemoglobin can reduce ischemia
Daisuke Shimbo, Takeo Abumiya, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Kiyohiro Houkin
BRAIN RESEARCH, 1554, 59, 66, Mar. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Changing Treatment Strategy From Clipping to Radial Artery Graft Bypass and Parent Artery Sacrifice in Patients With Ruptured Blister-Like Internal Carotid Artery Aneurysms
Ken Kazumata, Naoki Nakayama, Toshitaka Nakamura, Hiroyasu Kamiyama, Shunsuke Terasaka, Kiyohiro Houkin
NEUROSURGERY, 10, 1, 66, 72, Mar. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Hemorrhagic onset of hemangioblastoma located in the dorsal medulla oblongata presenting with tako-tsubo cardiomyopathy and neurogenic pulmonary edema: A case report
Masayuki Gekka, Shigeru Yamaguchi, Ken Kazumata, Hiroyuki Kobayashi, Hiroaki Motegi, Shunsuke Terasaka, Kiyohiro Houkin
Case Reports in Neurology, 6, 1, 68, 73, S. Karger AG, 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Intradural arachnoid cyst associated with syringomyelia: A case report
Yukitomo Ishi, Takeshi Aoyama, Kota Kurisu, Kazutoshi Hida, Kiyohiro Houkin
Neurological Surgery, 42, 5, 467, 472, Igaku-Shoin Ltd, 2014, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Clinical significance of STA-MCA double anastomosis for hemodynamic compromise in post-JET/COSS era
Satoshi Kuroda, Masahito Kawabori, Kenji Hirata, Tohru Shiga, Daina Kashiwazaki, Kiyohiro Houkin, Nagara Tamaki
ACTA NEUROCHIRURGICA, 156, 1, 77, 83, Jan. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Increased expression of vascular endothelial growth factor and its receptor in enlarging brain arteriovenous malformations - a case report
Daina Kashiwazaki, Rina Kobayashi, Kiyohiro Houkin, Satoshi Kuroda
BRITISH JOURNAL OF NEUROSURGERY, 28, 1, 119, 121, Jan. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

SPIOでラベリングした骨髄間質細胞,脳梗塞再生医療のソースとしての長期安全性
譚成博, 七戸秀夫, 寳金清博
再生医療, 13, 2014

Platelet lysateで培養した骨髄間質細胞移植による脳梗塞再生医療
七戸秀夫, 寳金清博
血液事業, 36, 4, 2014

脊髄硬膜動静脈瘻患者における痛みの質的評価
笹森徹, 関俊隆, 矢野俊介, 飛騨一利, 齋藤久壽, 寳金清博
日本脊髄外科学会プログラム・抄録集, 29th, 2014

特発性脊髄空洞症の臨床像
伊師雪友, 関俊隆, 飛騨一利, 笹森徹, 寳金清博
日本脊髄外科学会プログラム・抄録集, 29th, 2014

ピオクタニンを用いたperineural cystの1手術例
東海林菊太郎, 東海林菊太郎, 飛騨一利, 関俊隆, 矢野俊介, 寳金清博
日本脊髄外科学会プログラム・抄録集, 29th, 2014

脊髄硬膜動静脈瘻患者における痛み,生活の質の調査
笹森徹, 関俊隆, 矢野俊介, 飛騨一利, 齋藤久壽, 寳金清博
日本脊髄外科学会プログラム・抄録集, 29th, 2014

Bioimaging in Cell Transplantation Therapy for Stroke
七戸秀夫, 寳金清博, 黒田敏
日本磁気共鳴医学会雑誌, 34, 2, 2014

Steps to the Clinical Testing of Bone Marrow Stromal Cell Transplantation for Stroke
七戸秀夫, 寳金清博
神経治療学, 31, 3, 2014

自家骨髄細胞移植による脳梗塞再生医療をめざして:日本発の前臨床/臨床試験ガイドラインの必要性
七戸秀夫, 寳金清博
再生医療, 13, 2014

脳梗塞に対する細胞治療,その神経保護効果について
七戸秀夫, 寳金清博, 黒田敏
日本脳循環代謝学会総会プログラム・抄録号, 26th, 2014

SPIOでラベリングした他家骨髄間質細胞の脳内投与による安全性
譚成博, 七戸秀夫, 鐙谷武雄, 中山若樹, 数又研, 穂刈正昭, 浜内祝嗣, 寳金清博
日本脳循環代謝学会総会プログラム・抄録号, 26th, 2014

新規医薬品・医療機器等の創出,難治性疾患の治療法の開発および最適な治療法の確立に関する研究 研究事業 1.新規培養法による自己骨髄間質細胞による脳梗塞の再生医療
寳金清博
新規医薬品・医療機器等の創出、難治性疾患の治療法の開発および最適な治療法の確立に関する研究 平成25年度 総括・分担研究報告書, 2014

治療戦略を目指した研究 再生・移植基礎研究 脳虚血後の神経再生
七戸秀夫, 寳金清博
日本臨床, 72, 2014

Intracranial anaplastic ependymoma with extraneural metastasis
遠藤将吾, 寺坂俊介, 茂木洋晃, 寳金清博
小児の脳神経, 38, 6, 2014

マウス局所脳虚血モデルに対する,シロスタゾールのROS産生抑制作用を介した脳保護効果
七戸秀夫, 鐙谷武雄, 中山若樹, 数又研, 穂刈正昭, 譚成博, 寳金清博, 黒田敏
日本脳循環代謝学会総会プログラム・抄録号, 26th, 2014

Ivy sign, misery perfusion, and asymptomatic moyamoya disease: FLAIR imaging and 15O-gas positron emission tomography
Sandra Vuignier, Masaki Ito, Kota Kurisu, Ken Kazumata, Naoki Nakayama, Hideo Shichinohe, Tohru Shiga, Jozsef Zoltan Kiss, Nagara Tamaki, Kiyohiro Houkin
Acta Neurochirurgica, 155, 11, 2097, 2104, 11, Nov. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Usefulness of FMISO-PET for Glioma Analysis
Hiroyuki Kobayashi, Kenji Hirata, Shigeru Yamaguchi, Shunsuke Terasaka, Tohru Shiga, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 53, 11, 773, 778, Nov. 2013, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Effective Surgical Revascularization Improves Cerebral Hemodynamics and Resolves Headache in Pediatric Moyamoya Disease
Masahito Kawabori, Satoshi Kuroda, Naoki Nakayama, Kenji Hirata, Toru Shiga, Kiyohiro Houkin, Nagara Tamaki
WORLD NEUROSURGERY, 80, 5, 612, 619, Nov. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

I-123-Iomazenil Single Photon Emission Computed Tomography Visualizes Recovery of Neuronal Integrity by Bone Marrow Stromal Cell Therapy in Rat Infarct Brain
Hisayasu Saito, Keiichi Magota, Songji Zhao, Naoki Kubo, Yuji Kuge, Hideo Shichinohe, Kiyohiro Houkin, Nagara Tamaki, Satoshi Kuroda
STROKE, 44, 10, 2869, 2874, Oct. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Long-term prognosis in patients with clipped unruptured cerebral aneurysmsaEuro center dot increased cerebrovascular events in patients with surgically treated unruptured aneurysms
Masaaki Hokari, Satoshi Kuroda, Naoki Nakayama, Kiyohiro Houkin, Tatsuya Ishikawa, Hiroyasu Kamiyama
NEUROSURGICAL REVIEW, 36, 4, 567, 571, Oct. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Totally Ossified Metaplastic Spinal Meningioma
Chang Il Ju, Kazutoshi Hida, Tomohiro Yamauchi, Kiyohiro Houkin
JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, 54, 3, 257, 260, Sep. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Diagnosis of moyamoya disease on magnetic resonance imaging: are flow voids in the basal ganglia an essential criterion for definitive diagnosis?
Takeshi Mikami, Toshiya Sugino, Shunya Ohtaki, Kiyohiro Houkin, Nobuhiro Mikuni
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 22, 6, 862, 8, Aug. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal, Flow voids in the basal ganglia cannot always be recognized on magnetic resonance imaging, even in patients with typical moyamoya disease. In this report, flow voids in the basal ganglia and cisternal flow voids of the sylvian valley were evaluated in patients with moyamoya disease, and their diagnostic value was verified. A total of 41 consecutive patients with moyamoya disease were included in this analysis. The number of flow voids in the basal ganglia and the sylvian valley were counted on each side by 3 observers. Then the numbers of flow voids were compared between the patients with moyamoya disease and controls. The patients with moyamoya disease had a significantly higher mean number of flow voids in the basal ganglia and the sylvian valley (P < .0001); however, the number of flow voids in the basal ganglia was 0 or 1 in 69 sides (28.0%) in patients with moyamoya disease. Comparative analysis using the area under the receiver operating curve indicated that the evaluation of flow voids in the sylvian valley was significantly superior method to that in the basal ganglia (P < .0001). The cutoff value for the number of cisternal flow voids in the sylvian valley for the diagnosis of moyamoya disease was 6. Based on these findings, we recommend a definitive diagnosis of moyamoya disease should include assessment for abnormal vessels around the terminal portions of the internal carotid arteries.

Arterial Spin-Labeling Magnetic Resonance Imaging After Revascularization of Moyamoya Disease
Toshiya Sugino, Takeshi Mikami, Kei Miyata, Kengo Suzuki, Kiyohiro Houkin, Nobuhiro Mikuni
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 22, 6, 811, 816, Aug. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Assessment of moyamoya disease using multidetector row computed tomography.
Toshiya Sugino, Takeshi Mikami, Shunya Ohtaki, Tohru Hirano, Satoshi Iihoshi, Kiyohiro Houkin, Nobuhiro Mikuni
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 22, 5, 644, 9, Jul. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal, The recent introduction of multidetector row computed tomography (MDCT) scanners has enabled high-resolution 3-dimensional reconstruction. The purpose of this study was to establish a method to evaluate moyamoya disease using computed tomography angiography (CTA), specifically MDCT. Twenty-four patients (48 sides total) with moyamoya disease diagnosed by magnetic resonance angiography (MRA) were evaluated by means of CTA using MDCT by 3 independent observers, and the resulting 144 sides were analyzed. CTA and MRA were compared in terms of the steno-occlusive changes exhibited in each vessel. CTA and MRA scores were assigned on the basis of the severity of occlusive changes in the internal carotid artery, middle cerebral artery, anterior cerebral artery, and posterior cerebral artery. CTA scores were significantly correlated with MRA scores (P < .0001), and the 2 scores were in complete agreement in 57 sides (39.6%). The mean CTA score was significantly lower than the mean MRA score (P < .0001). Compared with CTA, MRA overestimated occlusion in 115 of the 576 vessels assessed. The mean MRA score was significantly higher in the overestimation group than in the good correlation group (P < .0001). CTA had a significantly higher rate of detection of moyamoya-affected vessels (P = .0001). Our data indicate that CTA using MDCT is a more reliable technique than MRA for diagnosing moyamoya disease. The ability to perform CTA quickly is a significant benefit for patients with moyamoya disease, particularly in pediatric and emergency cases.

A modified Essen stroke risk score for predicting recurrent cardiovascular events: development and validation
Shigeki Sumi, Hideki Origasa, Kiyohiro Houkin, Yasuo Terayama, Shinichiro Uchiyama, Hiroyuki Daida, Hiroshi Shigematsu, Shinya Goto, Kortaro Tanaka, Susumu Miyamoto, Kazuo Minematsu, Masayasu Matsumoto, Yasushi Okada, Motoki Sato, Norihiro Suzuki
INTERNATIONAL JOURNAL OF STROKE, 8, 4, 251, 257, Jun. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Primary intracranial yolk sac tumor in the posterior fossa: Case report of a child with Down syndrome
Shogo Endo, Hiroyuki Kobayashi, Shunsuke Terasaka, Akihiro Iguchi, Yuko Cho, Junjiro Ohshirma, Kanako Kubota, Kiyohiro Houkin
CLINICAL NEUROLOGY AND NEUROSURGERY, 115, 6, 811, 813, Jun. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Reversible isolated accessory nerve palsy due to a large thrombosed vertebral aneurysm
Hisayasu Saito, Satoshi Kuroda, Shunsuke Terasaka, Takeshi Asano, Naoki Nakayama, Kiyohiro Houkin
Case Reports in Neurology, 5, 2, 135, 138, 2, May 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Sarpogrelate Dilates Cerebral Arteries in the Absence of Exogenous Serotonin
Maiko Kawamura, Masanori Ishiguro, Takashi Nagamine, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 53, 5, 291, 298, May 2013, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Thoracolumbar intramedullary subependymoma with multiple cystic formation: a case report and review
Motoyuki Iwasaki, Kazutoshi Hida, Takeshi Aoyama, Kiyohiro Houkin
EUROPEAN SPINE JOURNAL, 22, S317, S320, May 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

IDH1 mutation as a potential novel biomarker for distinguishing pseudoprogression from true progression in patients with glioblastoma treated with temozolomide and radiotherapy
Hiroaki Motegi, Yuuta Kamoshima, Shunsuke Terasaka, Hiroyuki Kobayashi, Shigeru Yamaguchi, Mishie Tanino, Junichi Murata, Kiyohiro Houkin
BRAIN TUMOR PATHOLOGY, 30, 2, 67, 72, Apr. 2013, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Primary rhabdoid tumor with low grade glioma component of the central nervous system in a young adult
Shogo Endo, Shunsuke Terasaka, Shigeru Yamaguchi, Hitoshi Ikeda, Tsutomu Kato, Hiroyuki Kobayashi, Shinya Tanaka, Kiyohiro Houkin
NEUROPATHOLOGY, 33, 2, 185, 191, Apr. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Timing and cell dose determine therapeutic effects of bone marrow stromal cell transplantation in rat model of cerebral infarct
Masahito Kawabori, Satoshi Kuroda, Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Yuji Kuge, Nagara Tamaki
NEUROPATHOLOGY, 33, 2, 140, 148, Apr. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Semiquantitative analysis of indocyanine green videoangiography for cortical perfusion assessment in superficial temporal artery to middle cerebral artery anastomosis
Haruto Uchino, Toshitaka Nakamura, Kiyohiro Houkin, Jun-ichi Murata, Hisatoshi Saito, Satoshi Kuroda
ACTA NEUROCHIRURGICA, 155, 4, 599, 605, Apr. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Microvascular decompression for hemifacial spasm associated with the vertebral artery.
Takeshi Mikami, Yoshihiro Minamida, Yukinori Akiyama, Masahiko Wanibuchi, Toshiya Sugino, Kiyohiro Houkin, Nobuhiro Mikuni
Neurosurgical review, 36, 2, 303, 8, Apr. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal, In patients with vascular compression syndromes, the preoperative recognition of the cranial nerves and compressed vessels will contribute to improved surgical results. The aim of this study was to clarify the pathophysiology of hemifacial spasm (HFS) associated with the vertebral artery (VA) and to assess the value of preoperative imaging. Fifty-three consecutive patients with HFS underwent microvascular decompression (MVD). Of these, 18 cases of HFS were associated with the VA; this case series was compared with the remaining non-VA-associated HFS. For preoperative assessment, magnetic resonance imaging (MRI) was performed. Since January 2006, fusion imaging has been performed by combining MRI and computed tomography angiography. Of the 18 cases of VA-associated HFS, 17 (94.4 %) were on the left side; this was significantly higher than in the non-VA-associated HFS cases (p < 0.0001). The VA was attached to the root exit zone (REZ) directly in three cases. In the other 15 cases, the VA was compressing the REZ indirectly as a result of other intervening vessels. In all cases, preoperative imaging fully simulated the status of the REZ. The symptom disappeared in 17 cases (94.4 %) after MVD, and there was no significant difference in the surgical results between the VA-associated group and the non-VA-associated group (p = 0.9925). HFS associated with VA is not a rare condition. Preoperative recognition is thus important to the improvement of surgical results. Fusion imaging is useful to determine the status of the REZ, especially in indirect VA-associated HFS.

難治性慢性硬膜下血腫で再発した嗅神経芽細胞腫の一例
伊師 雪友, 寺坂 俊介, 茂木 洋晃, 栗栖 宏多, 小林 浩之, 寳金 清博, 山田 洋介, 畑中 佳奈子
北海道医学雑誌, 88, 2-3, 111, 111, 北海道医学会, Apr. 2013, [Peer-reviewed]
Japanese

くも膜下出血と脳動脈瘤の最新情報 未破裂脳動脈瘤の治療適応と合併症
中山若樹, 寳金清博
Clin Neurosci, 31, 4, 408, 410, 01 Apr. 2013
Japanese

骨髄間質細胞移植によるラクナ梗塞モデルの中枢神経再生
七戸秀夫, 黒田敏, 宮本倫行, 山内朋裕, 斎藤久泰, 新保大輔, 数又研, 中山若樹, 寶金清博
再生医療, 12, 237, 28 Feb. 2013
Japanese

Strategy for the treatment of large-giant aneurysms in the cavernous portion of the internal carotid artery
Shunya Ohtaki, Takeshi Mikami, Satoshi Iihoshi, Kei Miyata, Tadashi Nonaka, Kiyohiro Houkin, Nobuhiro Mikuni
Neurological Surgery, 41, 2, 107, 115, 2, Feb. 2013, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Long-term clinical and radiological results of endovascular internal trapping in vertebral artery dissection
Daina Kashiwazaki, Satoshi Ushikoshi, Takeshi Asano, Satoshi Kuroda, Kiyohiro Houkin
NEURORADIOLOGY, 55, 2, 201, 206, Feb. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Incidence, Locations, and Longitudinal Course of Silent Microbleeds in Moyamoya Disease A Prospective T2*-Weighted MRI Study
Satoshi Kuroda, Daina Kashiwazaki, Tatsuya Ishikawa, Naoki Nakayama, Kiyohiro Houkin
STROKE, 44, 2, 516, 518, Feb. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Post-ischemic Intra-arterial Infusion of Liposome-encapsulated Hemoglobin Can Reduce Ischemic Reperfusion Injury
Shimbo Daisuke, Abumiya Takeo, Shichinohe Hideo, Nakayama Naoki, Kazumata Ken, Houkin Kiyohiro, Ishizuka Takanobu
STROKE, 44, 2, Feb. 2013, [Peer-reviewed]

Predictors Of Effective Indirect Bypass In Adult Moyamoya Disease
Miyamoto Michiyuki, Kuroda Satoshi, Nakayama Naoki, Kazumata Ken, Houkin Kiyohiro
STROKE, 44, 2, Feb. 2013, [Peer-reviewed]

Impaired Cerebrovascular Reactivity to Acetazolamide in Parent Arterial Territory of Major Artery Aneurysms in Adult Patients with Moyamoya Disease
Ito Masaki, Houkin Kiyohiro, Niiya Yoshimasa, Uchino Haruto, Mabuchi Shoji, Nakayama Naoki, Kazumata Ken, Shichinohe Hideo, Sugiyama Taku, Ishii Nobuaki, Nomura Mikio
STROKE, 44, 2, Feb. 2013, [Peer-reviewed]

Bone marrow stromal cell transplantation enhances recovery of motor function after lacunar stroke in rats
Hideo Shichinohe, Tomohiro Yamauchi, Hisayasu Saito, Kiyohiro Houkin, Satoshi Kuroda
Acta Neurobiologiae Experimentalis, 73, 3, 354, 363, 3, 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Translational challenge for bone marrow stroma cell therapy after stroke
Satoshi Kuroda, Kiyohiro Houkin
Frontiers of Neurology and Neuroscience, 32, 62, 68, S. Karger AG, 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Validity of Dual MRI and F-18-FDG PET Imaging in Predicting Vulnerable and Inflamed Carotid Plaque
Hisayasu Saito, Satoshi Kuroda, Kenji Hirata, Keiichi Magota, Tohru Shiga, Nagara Tamaki, Daisuke Yoshida, Satoshi Terae, Naoki Nakayama, Kiyohiro Houkin
CEREBROVASCULAR DISEASES, 35, 4, 370, 377, 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Transformation from asymptomatic to symptomatic of lower cervical spinal dural arteriovenous fistula.
Toru Sasamori, Kazutoshi Hida, Takeshi Asano, Toshiya Osanai, Syunsuke Yano, Takeshi Aoyama, Yoshinobu Iwasaki, Kiyohiro Houkin
Neurologia medico-chirurgica, 53, 2, 103, 6, 2, 2013, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, A 60-year-old woman presented with a spinal dural arteriovenous fistula (SDAVF) located in the lower cervical region, which had been asymptomatic for 56 months after the initial detection. She underwent embolization of the SDAVF when she became symptomatic, but her neurological recovery was only partial. Cervical SDAVF is rare but sometimes discovered in an asymptomatic state. The natural history and validity of preventive treatment for asymptomatic SDAVF have not been fully established. Her unfavorable outcome demonstrates the potential advantages of adequate treatment at an early stage even for asymptomatic SDAVF.

Clinical features of patients bearing central nervous system hemangioblastoma in von Hippel-Lindau disease
Hiroshi Kanno, Jun-ichi Kuratsu, Ryo Nishikawa, Kazuhiko Mishima, Atushi Natsume, Toshihiko Wakabayashi, Kiyohiro Houkin, Shunsuke Terasaka, Taro Shuin
ACTA NEUROCHIRURGICA, 155, 1, 1, 7, Jan. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Bone marrow stromal cell transplantation enhances recovery of local glucose metabolism after cerebral infarction in rats: A serial 18F-FDG PET study
Michiyuki Miyamoto, Satoshi Kuroda, Songji Zhao, Keiichi Magota, Hideo Shichinohe, Kiyohiro Houkin, Yuji Kuge, Nagara Tamaki
Journal of Nuclear Medicine, 54, 1, 145, 150, Jan. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

北海道版脳卒中地域連携クリティカルパス「脳卒中あんしん連携ノート」の施行状況
齊藤正樹, 板本孝治, 中村正弘, 三國信啓, 中川原譲二, 三浦哲嗣, 寳金清博, 深津恵美
北海道医報, 1134, 2013

脊髄動静脈奇形と脊髄硬膜動静脈瘻における病態と外科治療
飛騨一利, 長内俊也, 青山剛, 矢野俊介, 関敏孝, 岩崎喜信, 寳金清博
脳神経外科ジャーナル, 22, Supplement, 2013

Platelet lysateで培養した骨髄間質細胞移植による脳梗塞再生医療
七戸秀夫, 寳金清博
血液事業, 36, 2, 2013

モヤモヤ病と脳循環代謝-Sense of Wonder-
寳金清博
脳循環代謝, 25, 1, 2013

骨髄間質細胞移植による脳梗塞再生医療:治療機序の分子細胞レベルでの検討
七戸秀夫, 山内朋裕, 斎藤久泰, 寳金清博, 黒田敏
脳循環代謝, 25, 1, 2013

自家骨髄間質細胞移植による脳梗塞再生医療をめざして
七戸秀夫, 寳金清博
神経治療学, 30, 5, 2013

嗅神経芽細胞腫に対する治療成績と治療戦略
茂木洋晃, 寺坂俊介, 小林浩之, 本間明宏, 加納里志, 畠山博充, 水町貴諭, 福田諭, 寳金清博
日本脳腫瘍の外科学会プログラム・抄録集, 18th, 2013

松果体部に生じた原発性横紋筋肉腫の一例
伊師雪友, 寺坂俊介, 山崎和義, 茂木洋晃, 小林浩之, 寳金清博, 井口晶裕, 長祐子, 大島淳二郎, 佐藤智信
日本脳腫瘍の外科学会プログラム・抄録集, 18th, 2013

脳梗塞治療のための自己骨髄間質細胞移植
七戸秀夫, 寳金清博
週刊日本医事新報, 4663, 2013

初期治療に難渋した未熟奇形腫の1例
寺坂俊介, 小林浩之, 茂木洋晃, 金子貞洋, 遠藤将吾, 寳金清博, 石津桂, 白土博樹
日本小児血液・がん学会雑誌, 50, 2, 2013

5年の経過で髄腔外への転移を認めた退形成性上衣腫の1症例
遠藤将吾, 寺坂俊介, 金子貞洋, 小林浩之, 浅岡克行, 鴨嶋雄大, 山口秀, 茂木洋晃, 畑中佳奈子, 寳金清博
小児の脳神経, 38, 1, 2013

Intraoperative Dual Monitoring During Carotid Endarterectomy Using Motor Evoked Potentials and Near-Infrared Spectroscopy
Haruto Uchino, Toshitaka Nakamura, Satoshi Kuroda, Kiyohiro Houkin, Jun-ichi Murata, Hisatoshi Saito
WORLD NEUROSURGERY, 78, 6, 651, 657, Dec. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Inadequate Communication Between Patients With Unruptured Cerebral Aneurysms and Neurosurgeons
Makoto Saito, Yoshimitsu Takahashi, Yayoi Yoshimura, Ayako Shima, Akio Morita, Kiyohiro Houkin, Takeo Nakayama, Kazuhiko Nozaki
NEUROLOGIA MEDICO-CHIRURGICA, 52, 12, 873, 877, Dec. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Spontaneous Echo Contrast and Thrombus Formation at the Carotid Bifurcation After Carotid Endarterectomy
Masahito Kawabori, Tetsuyuki Yoshimoto, Masaki Ito, Shin Fujimoto, Taisei Mikami, Mutsuko Muraki, Sadao Kaneko, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 52, 12, 885, 891, Dec. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Experience of I-123-iomazenil SPECT study for crossed cerebellocerebral diaschisis: Report of two cases
Kota Kurisu, Masahito Kawabori, Yoshimasa Niiya, Yuzuru Ohta, Naoki Nakayama, Satoshi Kuroda, Shoji Mabuchi, Kiyohiro Houkin
CLINICAL NEUROLOGY AND NEUROSURGERY, 114, 9, 1274, 1276, Nov. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Bilateral Chronic Subdural Hematomas of the Posterior Fossae -Case Report-
Kota Kurisu, Masahito Kawabori, Yoshimasa Niiya, Yuzuru Ohta, Shoji Mabuchi, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 52, 11, 822, 825, Nov. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

人工酸素運搬体Liposome‐encapsulated hemoglobinを用いた虚血再灌流傷害の治療戦略
新保大輔, 鐙谷武雄, 七戸秀夫, 中山若樹, 数又研, 宝金清博, 石塚隆伸
脳循環代謝, 24, 1, 178, 178, (一社)日本脳循環代謝学会, Nov. 2012
Japanese

Predictors and Clinical Features of Postoperative Hyperperfusion after Surgical Revascularization for Moyamoya Disease A Serial Single Photon Emission CT/Positron Emission Tomography Study
Haruto Uchino, Satoshi Kuroda, Kenji Hirata, Tohru Shiga, Kiyohiro Houkin, Nagara Tamaki
STROKE, 43, 10, 2610, 2616, Oct. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Transplanted bone marrow stromal cells protect neurovascular units and ameliorate brain damage in stroke-prone spontaneously hypertensive rats
Masaki Ito, Satoshi Kuroda, Taku Sugiyama, Katsuhiko Maruichi, Masahito Kawabori, Naoki Nakayama, Kiyohiro Houkin, Yoshinobu Iwasaki
NEUROPATHOLOGY, 32, 5, 522, 533, Oct. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Hemorrhagic onset of rhabdoid meningioma after initiating treatment for infertility
Hiroaki Motegi, Hiroyuki Kobayashi, Shunsuke Terasaka, Nobuaki Ishii, Masaki Ito, Daisuke Shimbo, Kanako Kubota, Kiyohiro Houkin
BRAIN TUMOR PATHOLOGY, 29, 4, 240, 244, Oct. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Systematic review of complications for proper informed consent (4) cerebrospinal fluid shunts for hydrocephalus and related disorders
Masaki Ito, Kiyohiro Houkin, Hisayasu Saito, Daisuke Shimbo, Hiroaki Motegi, Masahito Kawabori, Michiyuki Miyamoto, Tomohiro Yamauchi
Neurological Surgery, 40, 10, 923, 945, 10, Oct. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Hemophilic Pseudotumor of the Temporal Bone With Conductive Hearing Loss -Case Report-
Daina Kashiwazaki, Shunsuke Terasaka, Yuuta Kamoshima, Kanako Kubota, Takeshi Asano, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 52, 10, 745, 747, Oct. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Radiation induced intraparenchymal meningioma occurring 6 years after CNS germinoma: Case report
Yuuta Kamoshima, Shunsuke Terasaka, Hiroyuki Kobayashi, Sadahiro Kaneko, Kanako Kubota, Shinya Tanaka, Kiyohiro Houkin
CLINICAL NEUROLOGY AND NEUROSURGERY, 114, 7, 1077, 1080, Sep. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Favorable Outcome After Radical Resection and Subsequent Local Irradiation of Malignant Peripheral Nerve Sheath Tumor in the Cervical Spine -Case Report-
Toru Sasamori, Kazutoshi Hida, Shunsuke Yano, Takeshi Aoyama, Takeshi Asano, Kanako Kubota, Manabu Ito, Kuniyoshi Abumi, Yoshinobu Iwasaki, Hisatoshi Saito, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 52, 9, 670, 674, Sep. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

[The Multi-detector-row CT angiography for diagnosis of spinal dural arteriovenous fistula].
Toshiya Osanai, Kazutoshi Hida, Takeshi Asano, Takeshi Aoyama, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 40, 8, 695, 703, 8, Aug. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, PURPOSE: Multi-detector-row CT angiography (MDCTA) is useful for diagnosis of spinal arteriovenous (AV) shunt. So we reported the role and pitfall of MDCTA based on our experience. SUBJECTS: From January 2007 to September 2011, 57 cases used MDCTA in our institution. We investigated 22 case of spinal dural AVF and 5 cases of epidural AVF. METHODS: All cases were performed by Toshiba Aquilion 64. FOV was the whole spine, curved planar reconstruction along the spinal canal, sagittal and axial view obtained by multi-planar reconstruction. RESULTS: MDCTA detected dilated the perimedullary vein in all cases and identified the level of segmental artery matching the result of DSA in 16 cases of spinal dural AVF (73%) and 1 cases of epidural AVF (20%). CONCLUSION: MDCTA is useful for screening, and identifying the shunt point, but care needs to be taken concerning the particular pitfall of MDCTA.

Bilateral abducens nerve palsies in treated cases of subarachnoid hemorrhage
Hisayasu Saito, Naoki Nakayama, Shugo Takikawa, Satoshi Ushikoshi, Daisuke Shinbo, Satoshi Kuroda, Kiyohiro Houkin
Neurological Surgery, 40, 8, 717, 722, 8, Aug. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Systematic review of complications for proper informed consent (3) surgery for unruptured middle cerebral artery aneurysm
Masahito Kawabori, Ken Kazumata, Kosuke Ohnishi, Taku Sugiyama, Masaki Itoh, Naoki Nakayama, Kiyohiro Houkin
Neurological Surgery, 40, 8, 731, 740, 8, Aug. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Pituitary Apoplexy Manifesting as Massive Intracerebral Hemorrhage -Case Report-
Kota Kurisu, Masahito Kawabori, Yoshimasa Niiya, Yuzuru Ohta, Shoji Mabuchi, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 52, 8, 587, 590, Aug. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

[A novel adherent culture method of glioblastoma cells expressing CD133 using collagen-1-coated plates].
Motegi H, Kamoshima Y, Terasaka S, Kobayashi H, Houkin K
[Hokkaido igaku zasshi] The Hokkaido journal of medical science, 87, 4-5, 147, 151, 4-5, Aug. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, BACKGROUND AND AIMS: Glioblastoma is one of the most malignant brain tumors, causing death within two years despite maximal tumor resection and concurrent radio-chemotherapy. Tumor stem cells are thought to be closely related to tumor progression and recurrence and are attractive therapeutic targets. It is common to culture cell lines in serum-free medium with growth factors to stimulate spheres of enriched tumor stem cells. To avoid spontaneous differentiation and cell death in the sphere environment, Pollard et al. formulated an adherent culture method using laminin-coated plates. We here evaluated collagen-1-coated plates, which are superior to laminin-coated plates in handling and cost, as an alternative adherent culture method of CD133 expressing glioblastoma cells. MATERIALS AND METHODS: We cultured the human glioblastoma cell line, U87MG, under serum contained medium (SCM) or serum free medium with EGF, FGF2, LIF, B27 and N2 supplements (SFM) in non-coated, laminin or collagen-1-coated plates. The growth morphology in various cultures was evaluated, and the number of living cells in each plate on day 5 after cell seeding was calculated. The RNA expression level of CD133 as a stem cell marker in each plate was examined. Semi-quantitative measurement of CD133 positive cells by immunocytochemistry was performed. RESULTS: In collagen-1-coated plates with SFM, cell lines were cultured in an adherent monolayer. Cell proliferation was statistically encouraged in collagen-1-coated plates. Both laminin-coated plates and collagen-1-coated plates with SFM enhanced the RNA expression of CD133 compared to non-coated plates with SCM. Immunocytochemistry showed a statistically significant increase of CD133 positive cells in collagen-1-coated plates with SFM. CONCLUSION: Collagen-1-coated plates with SFM was used for cell morphology and cell proliferation of CD133 expression in the U87MG malignant glioma cell line.

クリッピング後長期間経過した後に再発した前交通動脈瘤とその臨床的意義
穂刈正昭, 中山若樹, 黒田敏, 長内俊也, 濱内祝嗣, 遠藤将吾, 宝金清博
脳神経外科速報, 22, 8, 938, 942, (株)メディカ出版, Aug. 2012
Japanese

Giant Calcified Thrombosed Varices Secondary to a Pial Arteriovenous Fistula Associated With Hereditary Hemorrhagic Telangiectasia -Case Report of Surgical Removal-
Taku Sugiyama, Naoki Nakayama, Shunsuke Terasaka, Satoshi Kuroda, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 52, 7, 506, 509, Jul. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

[The utility and efficacy of preoperative embolization for hypervascular tumor by NBCA].
Toshiya Osanai, Takeshi Asano, Shunsuke Terasaka, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 40, 7, 599, 606, 7, Jul. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, Preoperative embolization for hypervascular tumors is typically performed using particle agents such as polyvinyl alcohol, gelfoam powder, and fibrin glue. Furthermore, n-butyl-2-cyanoacrylate (NBCA) is used in patients with cerebral arteriovenous malformation (AVM) or an AV shunt. However, few reports have described the efficacy of NBCA in treating hypervascular tumors. Here, we report cases of hypervascular tumors in which preoperative embolization was performed using NBCA. We also discuss the difference between the efficacy of a liquid agent and a particle agent for hypervascular tumor embolization. We analyzed 10 cases encountered at our institution since 2004 in which preoperative embolization was performed using NBCA. In all cases, NBCA was injected through the tumor-feeding artery. In eight of these cases, preoperative embolization decreased intraoperative bleeding and markedly reduced the tumor stain. In the remaining two cases, complications occurred but without any permanent sequel. Thus, NBCA is useful for preoperative embolization.

Primary clear cell carcinoma of the skull base and paranasal cavity: A case report
Kengo Suzuki, Masahiko Wanibuchi, Yukinori Akiyama, Jun Ikeda, Yoshihiro Minamida, Tadashi Hasegawa, Kiyohiro Houkin, Nobuhiro Mikuni
Neurological Surgery, 40, 7, 617, 621, 7, Jul. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

[Treatment strategy of a patient who has multiple cerebral aneurysms caused by subarachnoid hemorrhage: possibility of coil embolization for aortic aneurysms].
Hokari M, Nagauchi T, Nakayama N, Hamauchi S, Ito Y, Shimoda Y, Houkin K
No shinkei geka. Neurological surgery, 40, 651, 655, 7, Jul. 2012, [Peer-reviewed]

[Treatment strategy of a patient with multiple cerebral aneurysms and subarachnoid hemorrhage: a case with both coil embolization suitable aneurysm and the unsuitable one].
Masaaki Hokari, Toshiya Osanai, Naoaki Nakayama, S Hamauchi, Yasuhiro Ito, Yusuke Shimoda, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 40, 7, 651, 5, Jul. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

神経・筋疾患の病態と診断・治療(I)脳動静脈奇形
中山若樹, 宝金清博
月刊医学と薬学, 68, 1, 30, 40, (株)自然科学社, Jul. 2012
Japanese

A case of vein of galen aneurysmal malformation with a newly developed dural arteriovenous fistula after successful embolization
Takeshi Asano, Satoshi Kuroda, Kiyohiro Houkin, Daisuke Yoshida, Kazutoshi Cho, Hideaki Shiraishi, Shinji Saito
Neurological Surgery, 40, 6, 519, 525, 6, 10 Jun. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

[Surgery for unruptured anterior cerebral artery aneurysms].
Sugiyama T, Houkin K, Ito M, Hokari M, Nakayama N, Kazumata K, Kuroda S
No shinkei geka. Neurological surgery, 40, 6, 555, 565, 6, Jun. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, In Japan, a large number of surgical treatments for unruptured intracranial aneurysms have been performed. On the other hands, it is known that surgical treatment for unruptured intracranial aneurysms is the most frequent source of lawsuits in the neurosurgical field. Neurosurgeons have the duty to disclose all risks and consequences of a proposed surgical procedure to each patient. Moreover, precise knowledge of surgical risks would be helpful in preventing complications from occurring. However, it is almost impossible that a single surgeon or an institute have experiences with all possible surgical complications because there is limitation of the number of surgery. In this review series, we attempted an exhaustive bibliographic survey of the possible surgical complications including exceptional ones, and then, determined the frequency of each complications as far as possible quantitatively. In this paper, we focused on the complications of surgical treatment for anterior cerebral artery aneurysms.

Intracerebral, but not intravenous, transplantation of bone marrow stromal cells enhances functional recovery in rat cerebral infarct: An optical imaging study
Masahito Kawabori, Satoshi Kuroda, Taku Sugiyama, Masaki Ito, Hideo Shichinohe, Kiyohiro Houkin, Yuji Kuge, Nagara Tamaki
NEUROPATHOLOGY, 32, 3, 217, 226, Jun. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

A cranial reconstruction using an autologous split calvarial bone combined with a free graft of temporal loose areolar tissue
Yuuta Kamoshima, Shunsuke Terasaka, Akihiko Oyama, Takehiro Warabi, Yusuke Shimoda, Kiyohiro Houkin
Neurological Surgery, 40, 5, 407, 412, 5, 10 May 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Review of Past Research and Current Concepts on the Etiology of Moyamoya Disease
Kiyohiro Houkin, Masaki Ito, Taku Sugiyama, Hideo Shichinohe, Naoki Nakayama, Ken Kazumata, Satoshi Kuroda
NEUROLOGIA MEDICO-CHIRURGICA, 52, 5, 267, 277, May 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Bypass Surgery for Moyamoya Disease -Concept and Essence of Surgical Techniques
Satoshi Kuroda, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 52, 5, 287, 294, May 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Transient Crossed Cerebellar Diaschisis Due to Cerebral Hyperperfusion Following Surgical Revascularization for Moyamoya Disease -Case Report
Masaaki Hokari, Satoshi Kuroda, Yusuke Simoda, Haruto Uchino, Kenji Hirata, Tohru Shiga, Naoki Nakayama, Kiyohiro Houkin, Nagara Tamaki
NEUROLOGIA MEDICO-CHIRURGICA, 52, 5, 350, 353, May 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

[Subclavian artery stenting using gadolinium contrast medium in a case with iodine allergy].
Michiyuki Miyamoto, Takeshi Asano, Toshiya Osanai, Shogo Endo, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 40, 5, 415, 20, 5, May 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, The authors reported a subclavian artery stenting (SAS) using gadolinium contrast medium. The patient was a 65-year-old female who presented dizziness and right upper extremity pain with movement. Digital subtraction angiography revealed right subclavian artery occlusion with subclavian steal phenomenon. We tried to treat this lesion using SAS. However, iodinated contrast medium caused the allergy in this patient and the treatment was discontinued. Therefore, SAS was performed with gadolinium contrast medium. Using gadolinium contrast medium, it is possible to confirm large arteries like innominate artery and subclavian artery. The stenting procedure was performed without complication. The usage of gadolinium contrast medium has the limit and some strategies are important to reduce the usage of gadolinium contrast medium in SAS. First, PercuSurge GuardWire® was placed in the right internal carotid artery to confirm the anatomy, to decide working angle, and to treat the common carotid artery in case of dissection. Second, a "U" shaped guide wire was placed in the distal end from the brachial artery. Guide wire from femoral side was able to pass the lesion at midpoint of the "U" shaped one. SAS using gadolinium contrast medium may be an alternative treatment if a patient with subclavian artery stenosis or occlusion is allergic to iodinated contrast medium.

F-18-Fluoromisonidazole positron emission tomography may differentiate glioblastoma multiforme from less malignant gliomas
Kenji Hirata, Shunsuke Terasaka, Tohru Shiga, Naoya Hattori, Keiichi Magota, Hiroyuki Kobayashi, Shigeru Yamaguchi, Kiyohiro Houkin, Shinya Tanaka, Yuji Kuge, Nagara Tamaki
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 39, 5, 760, 770, May 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

One-Year Atherothrombotic Vascular Events Rates in Outpatients with Recent Non-Cardioembolic Ischemic Stroke: The EVEREST (Effective Vascular Event REduction after STroke) Registry
Norihiro Suzuki, Motoki Sato, Kiyohiro Houkin, Yasuo Terayama, Shinichiro Uchiyama, Hiroyuki Daida, Hiroshi Shigematsu, Shinya Goto, Kortaro Tanaka, Hideki Origasa, Susumu Miyamoto, Kazuo Minematsu, Masayasu Matsumoto, Yasushi Okada
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 21, 4, 245, 253, May 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Systematic review of complications for proper informed consent (1) surgery for unruptured internal carotid artery aneurysm
Kiyohiro Houkin, Masaki Ito, Michiyuki Miyamoto, Masaaki Hokari, Ken Kazumata, Naoki Nakayama, Satoshi Kuroda
Neurological Surgery, 40, 4, 365, 375, 4, 10 Apr. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

合併症のシステマティック・レビュー—適切なInformed Consentのために(1)未破裂内頚動脈瘤の外科治療
寳金清博, 伊東雅基, 宮本倫行, 穂刈正昭, 数又研, 中山若樹, 黒田敏
Neurol Surg, 40, 4, 365-375, 10 Apr. 2012
Japanese

The Impact of Extent of Resection and Histological Subtype on the Outcome of Adult Patients with High-grade Gliomas
Shigeru Yamaguchi, Hiroyuki Kobayashi, Shunsuke Terasaka, Nobuaki Ishii, Jun Ikeda, Hiromi Kanno, Hiroshi Nishihara, Shinya Tanaka, Kiyohiro Houkin
JAPANESE JOURNAL OF CLINICAL ONCOLOGY, 42, 4, 270, 277, Apr. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Impact of ageing on biological features of bone marrow stromal cells (BMSC) in cell transplantation therapy for CNS disorders: functional enhancement by granulocyte-colony stimulating factor (G-CSF).
Yasuhiro Chiba, Satoshi Kuroda, Toshiya Osanai, Hideo Shichinohe, Kiyohiro Houkin, Yoshinobu Iwasaki
Neuropathology : official journal of the Japanese Society of Neuropathology, 32, 2, 139, 48, 2, Apr. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal, This study was designed to clarify the effects of donor age on biological features of bone marrow stromal cells (BMSC), one of the candidates for cell transplantation therapy for CNS disorders, because many aged patients might require such therapy. This study was also aimed to test whether ex vivo treatments with granulocyte-colony stimulating factor (G-CSF) could modify biological properties of BMSC from aged donors and enhance its therapeutic effects in an animal model of traumatic brain injury. The BMSC were harvested from young (6-week-old) and aged (100-week-old) rats. The ageing significantly increased the senescence-associated β-galactosidase (SA-β-gal) activity of the cultured BMSC, and decreased their proliferative capacity and production of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). As the next step, the rats were subjected to brain freezing injury by applying liquid nitrogen onto the neocortex through the thinned skull. The 6-week BMSC, 100-week BMSC, G-CSF-treated 100-week BMSC or vehicle were stereotactically injected into the ipsilateral striatum at 7 days post-injury. Transplantation of the 6-week BMSC, but not 100-week BMSC, significantly improved locomotor function. However, treatment of the 100-week BMSC with 0.1 µmol of G-CSF significantly improved their proliferation activity and growth factor production, and recovered therapeutic effects in the injured brain. In conclusion, donor age may largely determine biological aspects of BMSC. G-CSF may contribute to improve the outcome of BMSC transplantation therapy for CNS disorders in aged patients.

A case of surgical treatment for giant hemangioblastoma in the dorsal medulla oblongata
Yuuta Kamoshima, Shunsuke Terasaka, Yusuke Shimoda, Hiroyuki Kobayashi, Satoshi Kuroda, Takeshi Asano, Shigeru Yamaguchi, Junichi Murata, Kiyohiro Houkin
Neurological Surgery, 40, 3, 229, 234, 3, 10 Mar. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

A case of direct carotid-cavernous fistula presenting with subarachnoid hemorrhage
Takeshi Asano, Kiyohiro Houkin, Takuya Moriwaki, Yoshimasa Niiya, Shyouji Mabuchi
Neurological Surgery, 40, 3, 235, 239, 3, 10 Mar. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Early Diagnosis and Surgical Revascularization for a Predictive Case of Moyamoya Disease in a Boy Born to a Moyamoya Mother
Hongyan Han, Satoshi Kuroda, Yusuke Shimoda, Kiyohiro Houkin
JOURNAL OF CHILD NEUROLOGY, 27, 3, 408, 413, Mar. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

甲状腺機能亢進症に合併した類もやもや病
三上 毅, 杉野 寿哉, 菅野 彩, 宝金 清博, 三國 信啓
脳卒中, 34, 2, 82, 88, (一社)日本脳卒中学会, Mar. 2012
Japanese

Visualization of the Superparamagnetic Iron Oxide (SPIO)-Labeled Bone Marrow Stromal Cells Using a 3.0-T MRI-a Pilot Study for Clinical Testing of Neurotransplantation
Hideo Shichinohe, Satoshi Kuroda, Kohsuke Kudo, Masaki Ito, Masahito Kawabori, Michiyuki Miyamoto, Mitsuhiro Nakanishi, Satoshi Terae, Kiyohiro Houkin
Translational Stroke Research, 3, 1, 99, 106, Mar. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Analyses of IDH1 mutation and MGMT promoter methylation status for 5 cases of long-term survivors with glioblastoma
Yuuta Kamoshima, Hiroaki Motegi, Shunsuke Terasaka, Hiroyuki Kobayashi, Shigeru Yamaguchi, Junichi Murata, Shinya Tanaka, Kiyohiro Houkin
Neurological Surgery, 40, 2, 129, 135, 2, 10 Feb. 2012, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Reoxygenation of Glioblastoma Multiforme Treated with Fractionated Radiotherapy Concomitant with Temozolomide: Changes Defined by F-18-fluoromisonidazole Positron Emission Tomography: Two Case Reports
Takuhito Narita, Hidefumi Aoyama, Kenji Hirata, Shunsuke Onodera, Tohru Shiga, Hiroyuki Kobayashi, Junichi Murata, Shunsuke Terasaka, Shinya Tanaka, Kiyohiro Houkin
JAPANESE JOURNAL OF CLINICAL ONCOLOGY, 42, 2, 120, 123, Feb. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Therapeutic effects of intra-arterial delivery of bone marrow stromal cells in traumatic brain injury of rats--in vivo cell tracking study by near-infrared fluorescence imaging.
Toshiya Osanai, Satoshi Kuroda, Taku Sugiyama, Masahito Kawabori, Masaki Ito, Hideo Shichinohe, Yuji Kuge, Kiyohiro Houkin, Nagara Tamaki, Yoshinobu Iwasaki
Neurosurgery, 70, 2, 435, 44, 2, Feb. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: A noninvasive and effective route of cell delivery should be established to yield maximal therapeutic effects for central nervous system (CNS) disorders. OBJECTIVE: To elucidate whether intra-arterial delivery of bone marrow stromal cells (BMSCs) significantly promotes functional recovery in traumatic brain injury (TBI) in rats. METHODS: Rat BMSCs were transplanted through the ipsilateral internal carotid artery 7 days after the onset of cortical freezing injury. The BMSCs were labeled with fluorescent dye, and in vivo optical imaging was employed to monitor the behaviors of cells for 4 weeks after transplantation. Motor function was assessed for 4 weeks, and the transplanted BMSCs were examined using immunohistochemistry. RESULTS: In vivo optical imaging and histologic analysis clearly demonstrated that the intra-arterially injected BMSCs were engrafted during the first pass without systemic circulation, and the transplanted BMSCs started to migrate from the cerebral capillary bed to the injured CNS tissue within 3 hours. Intra-arterial BMSC transplantation significantly promoted functional recovery after cortical freezing injury. A subgroup of BMSCs expressed the phenotypes of neurons, astrocytes, and endothelial cells around the injured neocortex 4 weeks after transplantation. CONCLUSION: Intra-arterial transplantation may be a valuable option for prompt, noninvasive delivery of BMSCs to the injured CNS tissue, enhancing functional recovery after TBI. In vivo optical imaging may provide important information on the intracerebral behaviors of donor cells by noninvasive, serial visualization.

Giant frontal mucocele occurring 32 years after frontal bone fracture: A case report
Yuuta Kamoshima, Shunsuke Terasaka, Yuji Nakamaru, Dai Takagi, Satoshi Fukuda, Kiyohiro Houkin
Case Reports in Neurology, 4, 1, 34, 37, 1, Jan. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Neurohypophyseal germinoma with abundant fibrous tissue
Shunsuke Terasaka, Masahito Kawabori, Hiroyuki Kobayashi, Junichi Murata, Hiromi Kanno, Shinya Tanaka, Kiyohiro Houkin
BRAIN TUMOR PATHOLOGY, 29, 1, 58, 62, Jan. 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Cerebrovascular Accident in Patients with Ventricular Assist Device
Shimoda Yusuke, Nakayama Naoki, Hokari Masaaki, Shitinohe Hideo, Kuroda Satoshi, Hokin Kiyohiro, Sakakibara Mamoru, Tsutsui Hiroyuki, Ooka Tomonori, Matsui Yoshiro
CEREBROVASCULAR DISEASES, 34, 109, 110, 2012, [Peer-reviewed]

Small but Severe Residual Hypoperfusion Relates to Symptomatic Hemorrhage Even after Early Perfusion Improvement in Tissue Plasminogen Activator Therapy
Takeo Abumiya, Masahito Katoh, Takuya Moriwaki, Masami Yoshino, Takeshi Aoki, Hiroyuki Imamura, Toshimitsu Aida, Naoki Nakayama, Kiyohiro Houkin
CEREBROVASCULAR DISEASES, 34, 5-6, 446, 447, 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

多発性頭蓋骨縫合早期癒合における導出静脈の同定における超音波検査の有用性
下田祐介, 黒田敏, 小山明彦, 浜内祝嗣, 山崎和義, 寳金清博
小児の脳神経, 37, 2, 2012

骨髄間質細胞移植による中枢神経再生医療のトランスレーショナルリサーチ
七戸秀夫, 黒田敏, 山内朋裕, 齋藤久泰, 新保大輔, 寳金清博
脳循環代謝, 24, 1, 2012

骨髄間質細胞移植による“Nursing Effect”-神経栄養因子の分泌による神経保護作用
七戸秀夫, 石原武, 黒田敏, 高橋浩治, 田中嘉一, 宮本倫行, 山内朋裕, 斎藤久泰, 武本浩, 寳金清博
再生医療, 11, 2012

Blood Flow Metabolism Mismatch Triggers Postoperative Hyperperfusion after Carotid Endarterectomy: A Serial SPECT/PET Study
Shimbo Daisuke, Kuroda Satoshi, Nakayama Naoki, Kazumata Ken, Hirata Kenji, Shiga Tohru, Houkin Kiyohiro, Tamaki Nagara
CEREBROVASCULAR DISEASES, 34, 19, 2012, [Peer-reviewed]

Full Exposure of Ruptured Cerebral Aneurysm Preceding Clip Application: A Novel Surgical Concept and Its Advantages in 118 Case Series
Nakayama Naoki, Houkin Kiyohiro, Ishikawa Tatsuya, Hokari Masaaki, Kazumata Ken, Ito Masaki, Sugiyama Taku, Yasuda Hiroshi
CEREBROVASCULAR DISEASES, 34, 20, 2012, [Peer-reviewed]

Topography of Hemorrhage and Microbleeds in Adult Moyamoya Disease
Kazumata Ken, Houkin Kiyohiro, Nakayama Naoki, Shichinohe Hideo, Osanai Toshiya, Shinpo Daisuke, Saito Hisayasu
CEREBROVASCULAR DISEASES, 34, 32, 2012, [Peer-reviewed]

Endovascular Treatment of Ruptured Arterial Dissection of the Vertebral Artery
Osanai Toshiya, Ushikoshi Satoshi, Ken Kazumata, Nakayama Naoki, Houkin Kiyohiro
CEREBROVASCULAR DISEASES, 34, 92, 2012, [Peer-reviewed]

Irregular Neointimal Lining with Prominent Proliferative Activity in a Patient Underwent Patch Angioplasty for Carotid Endarterectomy - An Autopsy Case Report
Ito Masaki, Niiya Yoshimasa, Uchino Haruto, Itosaka Hiroyuki, Shichinohe Hideo, Kazumata Ken, Nakayama Naoki, Mabuchi Syoji, Houkin Kiyohiro
CEREBROVASCULAR DISEASES, 34, 102, 2012, [Peer-reviewed]

骨髄間質細胞移植は脳梗塞周囲の神経受容体機能を改善させる―¹²³I‐iomazenil SPECTによる検討　　　　　　　　　　　　　　　
齋藤久泰, 黒田敏, 宮本倫行, 山内朋裕, 七戸秀夫, 趙松吉, 孫田惠一, 久保直樹, 久下裕司, 玉木長良, 寶金清博
脳循環代謝, 24, 1, 216, 2012
English, Research society

脳梗塞をターゲットとした骨髄間質細胞移植―新たな培養・移植・イメージング技術による展開　　　　　　　　　　　　　　　
黒田敏, 宮本倫行, 山内朋裕, 斉藤久泰, 伊東雅基, 川堀真人, 杉山拓, 千葉泰弘, 七戸秀夫, 宝金清博, 久下裕司, 趙松吉, 玉木長良
再生医療, 11, 136, 2012
Japanese, Research society

A possible mechanism of isolated oculomotor nerve palsy by apoplexy of pituitary adenoma without cavernous sinus invasion: a report of two cases
Hiroyuki Kobayashi, Masahito Kawabori, Shunsuke Terasaka, Junichi Murata, Kiyohiro Houkin
ACTA NEUROCHIRURGICA, 153, 12, 2453, 2456, Dec. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Peritoneal catheter placement into the suprahepatic space: A report of 2 cases with abdominal complications of ventriculoperitoneal shunts
Yukitomo Ishi, Masaki Ito, Shunsuke Terasaka, Hiroaki Motegi, Daisuke Shinbo, Sadahiro Kaneko, Kiyohiro Houkin
Neurological Surgery, 39, 11, 1061, 1066, 11, 10 Nov. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

A successful treatment for a case of idiopathic syringomyelia with syrinx located in the anterior horn
Kota Kurisu, Kazutoshi Hida, Takeshi Aoyama, Kiyohiro Houkin
Neurological Surgery, 39, 11, 1079, 1084, 11, 10 Nov. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

A case of hemifacial spasm in a juglar foramen tumor patient treated by microvascular decompression
Yuuta Kamoshima, Shunsuke Terasaka, Yusuke Shimoda, Satoshi Fukuda, Kiyohiro Houkin
Neurological Surgery, 39, 11, 1099, 1103, 11, 10 Nov. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Bone Marrow-Derived Endothelial Progenitor Cells Participate in the Initiation of Moyamoya Disease
Taku Sugiyama, Satoshi Kuroda, Naoki Nakayama, Shinya Tanaka, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 51, 11, 767, 773, Nov. 2011, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Giant Skull Base Atypical Meningioma Presenting With Rapidly Progressive Impaired Consciousness Caused by Severe Venous Congestion-Case Report
Shigeru Yamaguchi, Shunsuke Terasaka, Hiroyuki Kobayashi, Katsuyuki Asaoka, Junichi Murata, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 51, 11, 789, 792, Nov. 2011, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Giant Cell Tumor of the Frontal Bone in a Girl-Case Report
Yuuta Kamoshima, Yutaka Sawamura, Tetsuaki Imai, Hiroshi Furukawa, Kanako Kubota, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 51, 11, 798, 800, Nov. 2011, [Peer-reviewed]
English, Scientific journal

慢性血行力学的脳虚血病変に対するEC‐IC bypassの長期成績—PETを用いた治療選択における10年間の検討—
川堀真人, 黒田敏, 中山若樹, 石川達哉, 宝金清博
脳循環代謝, 23, 1, 155, 155, (一社)日本脳循環代謝学会, Nov. 2011
Japanese

高度に脳灌流圧が低下した領域における脳血液量の意義—OEFとAcetazolamideに対するCBV反応性
伊東雅基, 黒田敏, 穂刈正昭, 中山若樹, 志賀哲, 玉木長良, 宝金清博
脳循環代謝, 23, 1, 117, 117, (一社)日本脳循環代謝学会, Nov. 2011
Japanese

もやもや病における血行再建術後過還流—PET/SPECTを用いた検討
内野晴登, 黒田敏, 中山若樹, 宝金清博
脳循環代謝, 23, 1, 157, 157, (一社)日本脳循環代謝学会, Nov. 2011
Japanese

頚動脈内膜剥離術後の過灌流の予測因子—acetazolamideか?OEFか?
新保大輔, 黒田敏, 中山若樹, 宝金清博
脳循環代謝, 23, 1, 123, 123, (一社)日本脳循環代謝学会, Nov. 2011
Japanese

ヨード過敏症を有する右鎖骨下動脈CTOに対してstent治療を行った1例
宮本倫行, 長内俊也, 浅野剛, 中山若樹, 黒田敏, 宝金清博
J Neuroendovascular Ther, 5, 4, 219, 219, (NPO)日本脳神経血管内治療学会, Nov. 2011
Japanese

骨髄間質細胞移植は脳梗塞後の局所糖代謝を改善する―小動物用PET/CTによる検討　　　　　　　　　　　　　　　
宮本倫行, 黒田敏, 七戸秀夫, 伊東雅基, 川堀真人, 趙松吉, 孫田恵一, 久下裕司, 玉木長良, 宝金清博
脳循環代謝, 23, 1, 142, 142, (一社)日本脳循環代謝学会, Nov. 2011
Japanese

A case of metastatic choroid plexus tumor from cholangiocellular carcinoma
Kota Kurisu, Yuuta Kamoshima, Shunsuke Terasaka, Hiroyuki Kobayashi, Kanako Kubota, Kiyohiro Houkin
Neurological Surgery, 39, 10, 991, 997, 10, 10 Oct. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

A case of symptomatic pituitary metastases from renal cell carcinoma
Yuuta Kamoshima, Shunsuke Terasaka, Shuji Hamauchi, Hiroyuki Kobayashi, Shogo Endo, Yukiko Kanno, Kimihiko Kimachi, Kiyohiro Houkin
Neurological Surgery, 39, 10, 999, 1004, 10, 10 Oct. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Xanthogranuloma of the Sellar Region of Children: Series of Five Cases and Literature Review
Yuuta Kamoshima, Yutaka Sawamura, Hiroaki Motegi, Kanako Kubota, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 51, 10, 689, 693, Oct. 2011, [Peer-reviewed]
English

Midsubtemporal Ridge as a Predictor of the Lateral Loop Formed by the Maxillary Nerve and Mandibular Nerve: A Cadaveric Morphological Study
Masahiko Wanibuchi, Gen Murakami, Taro Yamashita, Yoshihiro Minamida, Takanori Fukushima, Allan H. Friedman, Mineko Fujimiya, Kiyohiro Houkin
NEUROSURGERY, 69, 1 Suppl Operative, 95, 98, Sep. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Sonodynamic therapy using water-dispersed TiO2-polyethylene glycol compound on glioma cells: Comparison of cytotoxic mechanism with photodynamic therapy
Shigeru Yamaguchi, Hiroyuki Kobayashi, Takuhito Narita, Koki Kanehira, Shuji Sonezaki, Nobuki Kudo, Yoshinobu Kubota, Shunsuke Terasaka, Kiyohiro Houkin
ULTRASONICS SONOCHEMISTRY, 18, 5, 1197, 1204, Sep. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Validity of Bone Marrow Stromal Cell Expansion by Animal Serum-Free Medium for Cell Transplantation Therapy of Cerebral Infarct in Rats—A Serial MRI Study
Masaki Ito, Satoshi Kuroda, Taku Sugiyama, Hideo Shichinohe, Yukari Takeda, Mitsufumi Nishio, Takao Koike, Kiyohiro Houkin
Translational Stroke Research, 2, 3, 294, 306, Springer Science and Business Media LLC, Sep. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal, This study was aimed to test the hypothesis that human bone marrow stromal cells (hBMSC) expanded in fetal calf serum (FCS)-free, platelet lysate (PL)-containing medium would retain their capacity of migration, survival, and neural differentiation when transplanted into the infarct brain, using serial in vivo magnetic resonance imaging (MRI). Cell growth kinetic analysis revealed that hBMSC maintain their proliferative activity when cultured either in conventional FCS-containing medium or FCS-free, PL-containing medium. Subsequently, hBMSC were labeled with a superparamagnetic iron oxide agent and were stereotactically transplanted into the ipsilateral striatum of rats at 7 days after permanent middle cerebral artery occlusion. Serial MRI performed over 8 weeks revealed that they retain their migratory capacity towards the cerebral infarct. Moreover, double fluorescence immunohistochemistry also revealed that they preserve their capacity of differentiation into the neural cells in the peri-infarct area. The hBMSC expanded in the FCS-free, PL-containing medium retain their capacity of migration, survival, and differentiation when stereotactically transplanted into the infarct brain. The present findings strongly suggest the clinical utility of PL as a substitute to expand autologous hBMSC for cerebral infarct in the future.

Biological Features of Human Bone Marrow Stromal Cells (hBMSC) Cultured with Animal Protein-Free Medium-Safety and Efficacy of Clinical Use for Neurotransplantation
Hideo Shichinohe, Satoshi Kuroda, Taku Sugiyama, Masaki Ito, Masahito Kawabori, Mitsufumi Nishio, Yukari Takeda, Takao Koike, Kiyohiro Houkin
Translational Stroke Research, 2, 3, 307, 315, Sep. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Intramedullary Cavernous Angiomas of the Spinal Cord : Clinical Characteristics of 13 Lesions
AOYAMA Takeshi, HIDA Kazutoshi, HOUKIN Kiyohiro
Neurologia medico-chirurgica, 51, 8, 561, 566, 15 Aug. 2011, [Domestic magazines]
English, Scientific journal

The repair of traumatic cerebrospinal fluid leak using temporal loose areolar connective tissue insertion into the frontal sinus and pericranial flap covering: A case report
Yuuta Kamoshima, Shunsuke Terasaka, Shuji Hamauchi, Sadahiro Kaneko, Hiroki Murakami, Kiyohiro Houkin
Neurological Surgery, 39, 8, 783, 788, 8, 10 Aug. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Primary Central Nervous System Mucosa-Associated Lymphoid Tissue Lymphoma : Case Report
KAMOSHIMA Yuuta, SAWAMURA Yutaka, SUGIYAMA Taku, YAMAGUCHI Shigeru, HOUKIN Kiyohiro, KUBOTA Kanako
Neurologia medico-chirurgica, 51, 7, 527, 530, 15 Jul. 2011, [Domestic magazines]
English, Scientific journal

Threshold levels of intracranial pressure as determinants of survival from diffuse brain injury. An observational study
Kei Miyata, Takeshi Mikami, Yasufumi Asai, Kazuhisa Mori, Izumi Koyanagi, Nobuhiro Mikuni, Kiyohiro Houkin
Neurological Surgery, 39, 7, 657, 662, 7, 10 Jul. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Association of Recurrent Cerebral Infarction With Adenosine Diphosphate- and Collagen-Induced Platelet Aggregation in Patients Treated With Ticlopidine and/or Aspirin
Takeo Abumiya, Kiyohiro Houkin
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 20, 4, 319, 323, Jul. 2011, [Peer-reviewed]
English, Scientific journal

Surgical approach for the tuberculum sellae meningioma: High and low?
Shunsuke Terasaka, Hiroyuki Kobayashi, Kiyohiro Houkin
Neurological Surgery, 39, 6, 553, 562, 6, 10 Jun. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Spontaneous disappearance of intracranial arteriovenous malformation after living-donor liver transplantation: A case report
Yusuke Shimoda, Satoshi Kuroda, Daina Kashiwazaki, Takeshi Asano, Ken-Ichiro Yamashita, Masahiko Taniguchi, Tomomi Suzuki, Tsuyoshi Shimamura, Hiroyuki Furukawa, Naoki Nakayama, Kiyohiro Houkin
Neurological Surgery, 39, 6, 589, 594, 6, 10 Jun. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

A case of ectopic pituitary adenoma occurring in the sphenoid sinus
Yuuta Kamoshima, Shunsuke Terasaka, Kazuki Uchida, Satoshi Ushikoshi, Keiryu Sou, Kiyohiro Houkin
Neurological Surgery, 39, 6, 601, 605, 6, 10 Jun. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Intravenous administration of auto serum-expanded autologous mesenchymal stem cells in stroke
Osamu Honmou, Kiyohiro Houkin, Takuya Matsunaga, Yoshiro Niitsu, Sumio Ishiai, Rie Onodera, Stephen G. Waxman, Jeffery D. Kocsis
BRAIN, 134, Pt 6, 1790, 1807, Jun. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Cranial reconstruction using autologous split calvarial bone combined with calcium phosphate bone cement: A case report
Yuuta Kamoshima, Shunsuke Terasaka, Akihiko Oyama, Kiyohiro Houkin
Neurological Surgery, 39, 5, 491, 495, 5, 10 May 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Sacral dural arteriovenous fistula: Report of 4 cases
Toru Sasamori, Kazutoshi Hida, Takeshi Asano, Takeshi Aoyama, Tomohiro Yamauchi, Motoyuki Iwasaki, Shunsuke Yano, Yoshinobu Iwasaki, Kiyohiro Houkin
Neurological Surgery, 39, 5, 505, 511, 5, 10 May 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

[A rare case of diffuse astrocytoma complicated with giant pigmented hairy nevi, suspected neurocutaneous melanosis].
Yuuta Kamoshima, Yutaka Sawamura, Nobuharu Saitoh, Masahito Kawabori, Kiyohiro Houkin, Kanako Kubota
No shinkei geka. Neurological surgery, 39, 5, 479, 83, 5, May 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, A 1-year-old female infant presented with congenital giant, hairy and pigmented nevi. MRI scan as screening test revealed a cerebellar tumor. A diagnosis of provisional neurocutaneous melanosis was made on the basis of the patient's MRI and physical findings. At her 6 years of age, MRI revealed the tumor grown up to 3 cm diameter in 5 years. The cerebellar tumor was removed partially using the occipital transtentorial approach for tissue diagnosis. The color of the cerebellar tumor was whitish and contained neither benign nor malignant melanocyte. Pathological examination revealed diffuse astrocytoma. Finally residual cerebellar tumor was totally removed at a second surgical resection. To our knowledge, this is the first patient to be reported with astrocytoma complicated giant skin nevus except neurocutaneous syndrome cases.

仙骨部硬膜動静脈瘻の4例
笹森 徹, 飛騨 一利, 浅野 剛, 青山 剛, 山内 朋裕, 岩崎 素之, 矢野 俊介, 岩崎 喜信, 寳金 清博
Neurological Surgery, 39, 5, 505, 511, (株)医学書院, May 2011
Japanese

Conus perimedullary arteriovenous fistula with multiple shunt points including the cauda equina: A case report
Hisayasu Saito, Kazutoshi Hida, Takeshi Asano, Shunsuke Yano, Takeshi Aoyama, Yoshinobu Iwasaki, Kiyohiro Houkin
Neurological Surgery, 39, 4, 375, 380, 4, 10 Apr. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

中大脳動脈瘤の術野展開とclipping work
中山若樹, 石川達哉, 宝金清博, 黒田敏, 上山博康
脳神経外科ジャーナル, 20, Supplement, 46, 10 Apr. 2011
Japanese

[Cranioplasty using autologous calvarial bone graft in combination with titanium mesh plates for patients with a history of bone infections after initial cranioplasty].
Yuuta Kamoshima, Yutaka Sawamura, Kunihiro Kawashima, Toshiya Osanai, Daina Kashiwazaki, Tomohiro Yamauchi, Kiyohiro Houkin
No shinkei geka. Neurological surgery, 39, 4, 351, 8, 4, Apr. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, We present a modified method for reconstruction of calvarial bone defects for patients with a history of infectious complications. Three patients who had experienced implanted bone infections underwent reconstruction of calvarial bone defect. For reconstruction of the calvarial bone defects, autologous split calvarial bone grafts were used to cover the calvarial bone defect. The full or half layered fronto-parietal bone used as implants were fixed with titanium mini-plates for primary bone defect site, while the new bone defect site caused by getting autologous bone graft were covered with titanium mesh plates assisted by residual half layered calvarias. The average follow-up span of patients was 64 months. Evaluated clinical and radiologic results are stable, showing no measurable side effects. Split calvarial bone graft in combination with titanium mesh plates is recommended in patients with a history of infection or high risk of infection.

Intraoperative Indocyanine Green Videoangiography for Spinal Vascular Lesions: Case Report
Tomohiro Murakami, Izumi Koyanagi, Takahisa Kaneko, Satoshi Iihoshi, Kiyohiro Houkin
NEUROSURGERY, 68, 3, E241, E245, Mar. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Intraoperative Indocyanine Green Videoangiography for Spinal Vascular Lesions: Case Report
Tomohiro Murakami, Izumi Koyanagi, Takahisa Kaneko, Satoshi Iihoshi, Kiyohiro Houkin
NEUROSURGERY, 68, Mar. 2011, [Peer-reviewed]
English, Scientific journal

The Incidence And Mechanisms Of Postoperative Cerebral Infarction After Clipping Surgery For Non-ruptured Intracranial Aneurysms - A Multivariate Analysis Study
Miyamoto Michiyuki, Kuroda Satoshi, Nakayama Naoki, Hokari Masaaki, Yasuda Hiroshi, Saito Hisatoshi, Houkin Kiyohiro
STROKE, 42, 3, E172, Mar. 2011, [Peer-reviewed]

Validity of F-18-fluorodeoxyglucose PET in Identifying Inflamed and Vulnerable Carotid Plaque - A Radiological and Pathological Study
Kuroda Satoshi, Maruichi Katsuhiko, Nakayama Naoki, Houkin Kiyohiro, Hirata Kenji, Shiga Tohru, Tamaki Nagara
STROKE, 42, 3, E183, Mar. 2011, [Peer-reviewed]

Invasive paranasal sinus fungal infection developing orbital apex syndrome and causing internal carotid artery infiltration: Reports of 3 cases
Taku Sugiyama, Satoshi Kuroda, Naoki Nakayama, Kiyohiro Houkin
Neurological Surgery, 39, 2, 155, 161, 2, 10 Feb. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Flow diversion by double-overlapping-stent for fusiform vertebral artery aneurysm: A case report
Haruto Uchino, Takeshi Asano, Naoki Nakayama, Satoshi Kuroda, Kiyohiro Houkin
Neurological Surgery, 39, 1, 59, 63, 1, Jan. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Susceptibility-weighted Imaging and Magnetic Resonance Angiography during Migraine Attack: A Case Report
Yusuke Shimoda, Kohsuke Kudo, Satoshi Kuroda, Yuri Zaitsu, Nobuyuki Fujima, Satoshi Terae, Makoto Sasaki, Kiyohiro Houkin
MAGNETIC RESONANCE IN MEDICAL SCIENCES, 10, 1, 49, 52, 2011, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Multiple infectious intracranial lesions of Mycobacterium genavense in an immunocompromised patient
Haruto Uchino, Shunsuke Terasaka, Shigeru Yamaguchi, Hiroyuki Kobayashi, Kagari Kawai, Kanako Kubota, Shinji Ooe, Kiyohiro Houkin
Brain and Nerve, 63, 1, 79, 83, 1, 01 Jan. 2011, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

日本脳卒中協会主催PCEC/PSLS講習の意義
大里俊明, 大里俊明, 齊藤正樹, 齊藤正樹, 齊藤正樹, 奈良理, 奈良理, 宮田圭, 宮田圭, 浅井康文, 浅井康文, 中川原譲二, 中川原譲二, 寳金清博, 寳金清博
日本脳神経外科救急学会プログラム・抄録集, 16th (CD-ROM), 2011

解剖を中心とした脳神経手術手技 鞍結節髄膜腫に対する手術到達法の選択:高位か低位か?
寺坂俊介, 小林浩之, 寳金清博
Neurological Surgery, 39, 6, 2011

急速に進行する虚血性もやもや病に対する早期血行再建術の有用性
栗栖宏多, 黒田敏, 中山若樹, 内野晴登, 中村俊孝, 寶金清博
脳循環代謝, 23, 1, 157, 2011
Japanese

もやもや病術後症例に対する追加脳血行再建術—その実際と脳循環代謝
黒田敏, 中山若樹, 平田健司, 志賀哲, 玉木長良, 宝金清博
脳循環代謝, 22, 1, 78, 78, (一社)日本脳循環代謝学会, Nov. 2010
Japanese

小児モヤモヤ病における頭痛と脳血行動態の関係について
川堀真人, 黒田敏, 中山若樹, 宝金清博
脳循環代謝, 22, 1, 80, 80, (一社)日本脳循環代謝学会, Nov. 2010
Japanese

Fracture of the cervical spine in ankylosing spondylitis. A case report
Takahisa Kaneko, Izumi Koyanagi, Tomohiro Murakami, Kiyohiro Houkin
Neurological Surgery, 38, 9, 839, 843, 9, 10 Sep. 2010, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Glioblastoma simultaneously present with adjacent meningioma: case report and review of the literature
Kengo Suzuki, Hiroyuki Momota, Akiko Tonooka, Hiroko Noguchi, Kouhei Yamamoto, Masahiko Wanibuchi, Yoshihiro Minamida, Tadashi Hasegawa, Kiyohiro Houkin
JOURNAL OF NEURO-ONCOLOGY, 99, 1, 147, 153, Aug. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor-induced neurite outgrowth in PC12 cells.
Toshiya Sugino, Mitsuhisa Maruyama, Masaya Tanno, Atsushi Kuno, Kiyohiro Houkin, Yoshiyuki Horio
FEBS letters, 584, 13, 2821, 6, 13, 02 Jul. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal, SIRT1, a NAD(+)-dependent protein deacetylase, is known to have neural functions. However, despite its cytoplasmic expression in some neural cells, its cytoplasmic function, if any, is unknown. Here we found that PC12 (pheochromocytoma) cells expressed SIRT1 in the cytoplasm. Nerve growth factor (NGF)-induced neurite outgrowth of these cells was promoted by activators of SIRT1, while inhibitors of SIRT1 or SIRT1-siRNA significantly inhibited it. The overexpression of a mutant SIRT1 that localised to the cytoplasm but not the nucleus enhanced the NGF-dependent neurite outgrowth, and a cytoplasmic dominant-negative SIRT1 suppressed it. Thus, cytoplasmic SIRT1 increases the NGF-induced neurite outgrowth of PC12 cells.

Pathogenesis of syringomyelia associated with Chiari type 1 malformation: review of evidences and proposal of a new hypothesis
Izumi Koyanagi, Kiyohiro Houkin
NEUROSURGICAL REVIEW, 33, 3, 271, 284, Jul. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Pulsatile wall movement of spinal arachnoid cyst deteriorates spinal cord symptoms: report of three cases
Takeo Baba, Izumi Koyanagi, Kazuhisa Yoshifuji, Tomohiro Murakami, Kiyohiro Houkin
ACTA NEUROCHIRURGICA, 152, 7, 1245, 1249, Jul. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Intravenous administration of mesenchymal stem cells derived from bone marrow after contusive spinal cord injury improves functional outcome
Misuzu Osaka, Osamu Honmou, Tomohiro Murakami, Tadashi Nonaka, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
BRAIN RESEARCH, 1343, 226, 235, Jul. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor-induced neurite outgrowth in PC12 cells
Toshiya Sugino, Mitsuhisa Maruyama, Masaya Tanno, Atsushi Kuno, Kiyohiro Houkin, Yoshiyuki Horio
FEBS LETTERS, 584, 13, 2821, 2826, Jul. 2010, [Peer-reviewed]
English, Scientific journal

Novel bypass surgery for moyamoya disease using pericranial flap: Its impacts on cerebral hemodynamics and long-term outcome
Satoshi Kuroda, Kiyohiro Houkin, Tatsuya Ishikawa, Naoki Nakayama, Yoshinobu Iwasaki
Neurosurgery, 66, 6, 1093, 1101, 6, Jun. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Stem cell therapy for cerebral ischemia
Osamu Honmou, Kiyohiro Houkin
Neurological Surgery, 38, 6, 515, 520, 6, Jun. 2010, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Angiographic analysis of variations and anomalous origin of the anterior choroidal artery and posterior communicating artery
Satoshi Iihoshi, Tadashi Nonaka, Kei Miyata, Kiyohiro Houkin
Neurological Surgery, 38, 6, 523, 530, 6, Jun. 2010, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Therapeutic time window of mesenchymal stem cells derived from bone marrow after cerebral ischemia
Katsuya Komatsu, Osamu Honmou, Junpei Suzuki, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
BRAIN RESEARCH, 1334, 84, 92, Jun. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Skull base training and education using an artificial skull model created by selective laser sintering
Masahiko Wanibuchi, Masafumi Ohtaki, Takanori Fukushima, Allan H. Friedman, Kiyohiro Houkin
ACTA NEUROCHIRURGICA, 152, 6, 1055, 1060, Jun. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Novel Bypass Surgery for Moyamoya Disease Using Pericranial Flap: Its Impacts on Cerebral Hemodynamics and Long-term Outcome
Satoshi Kuroda, Kiyohiro Houkin, Tatsuya Ishikawa, Naoki Nakayama, Yoshinobu Iwasaki
NEUROSURGERY, 66, 6, 1093, 1101, Jun. 2010, [Peer-reviewed]
English, Scientific journal

小児もやもや病の外科治療—今後の課題
黒田敏, 笹森徹, 川堀真人, 中山若樹, 宝金清博, 石川達哉
小児の脳神経, 35, 2, 183, 183, (一社)日本小児神経外科学会, Apr. 2010
Japanese

Cysteinyl leukotriene receptor antagonists inhibit tumor metastasis by inhibiting capillary permeability
Masako Nozaki, Masanobu Yoshikawa, Kunihiko Ishitani, Hiroyuki Kobayashi, Kiyohiro Houkin, Kohzoh Imai, Yoichiro Ito, Takamura Muraki
Keio Journal of Medicine, 59, 1, 10, 18, 1, Mar. 2010, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Practical Decision-Making in the Treatment of Unruptured Cerebral Aneurysm in Japan: The U-CARE Study
Yukinori Akiyama, Kiyohiro Houkin, Kazuhiko Nozaki, Nobuo Hashimoto
CEREBROVASCULAR DISEASES, 30, 5, 491, 499, 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Therapeutic benefits of human mesenchymal stem cells derived from bone marrow after global cerebral ischemia
Wei Zheng, Osamu Honmou, Kei Miyata, Kuniaki Harada, Junpei Suzuki, He Liu, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
BRAIN RESEARCH, 1310, 8, 16, Jan. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Hyperperfusion syndrome after clipping an unruptured cerebral aneurysm: two case reports.
Toshiya Sugino, Masafumi Ohtaki, Masahiko Wanibuchi, Sonen Kin, Kiyohiro Houkin
Neurologia medico-chirurgica, 50, 4, 306, 9, 4, 2010, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, A 68-year-old woman presented with severe headache 9 days after undergoing successful clipping of a right middle cerebral artery aneurysm. Postoperative imaging revealed increased perfusion and diffuse edema in the right frontotemporal cortex. A 57-year-old woman exhibited perseveration soon after undergoing successful clipping of an anterior communicating artery aneurysm. Postoperative imaging studies revealed increased perfusion and diffuse edema in the left frontal and insular cortex. The symptoms and diffuse edema gradually resolved in both patients. These two cases of hyperperfusion syndrome occurred in a series of 190 patients treated by clipping of unruptured cerebral aneurysms. Hyperperfusion syndrome is a rare complication following aneurysm surgery, especially surgery for unruptured cerebral aneurysms without temporary clipping.

脳虚血に対する幹細胞移植
本望修, 本望修, 寳金清博
Neurological Surgery, 38, 6, 2010

多椎間後方固定を必要とした腰椎破壊性脊椎関節症の1例
村上友宏, 小柳泉, 金子高久, 寳金清博, 恩田敏之, 八巻稔明
日本脊髄外科学会プログラム・抄録集, 25th, 2010

脳卒中教育としてのNIH stroke scaleの卒前医学教育とOSCE導入,卒業試験実施報告
齊藤正樹, 山本和利, 寳金清博, 米増保之, 高橋明, 小松克也, 小松克也, 三上毅, 三上毅, 今井富裕, 矢坂正弘, 岡田靖, 佐藤利夫, 下濱俊
日本神経学会総会プログラム・抄録集, 51st, 2010

穿通性頭蓋底損傷について
三上毅, 秋山幸功, 宮田圭, 南田善弘, 寳金清博
日本脳神経外科救急学会プログラム・抄録集, 15th, 2010

Preoperative and postoperative MRA
Takeshi Mikami, Satoshi Iihoshi, Kiyohiro Houkin
Moyamoya Disease Update, 150, 157, Springer Japan, 2010, [Peer-reviewed]
English, In book

Overview of image diagnosis of moyamoya disease
Kiyohiro Houkin, Satoshi Iihoshi, Takeshi Mikami
Moyamoya Disease Update, 141, 149, Springer Japan, 2010, [Peer-reviewed]
English, In book

BDNF-Hypersecreting Human Mesenchymal Stem Cells Promote Functional Recovery, Axonal Sprouting, and Protection of Corticospinal Neurons after Spinal Cord Injury
Masanori Sasaki, Christine Radtke, Andrew M. Tan, Peng Zhao, Hirofumi Hamada, Kiyohiro Houkin, Osamu Honmou, Jeffery D. Kocsis
JOURNAL OF NEUROSCIENCE, 29, 47, 14932, 14941, Nov. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Prior Ethanol Injection Promotes Brain Edema after Traumatic Brain Injury
Ryuichi Katada, Yoko Nishitani, Osamu Honmou, Shunichiro Okazaki, Kiyohiro Houkin, Hiroshi Matsumoto
JOURNAL OF NEUROTRAUMA, 26, 11, 2015, 2025, Nov. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

QUANTITATIVE ANALYSIS OF ADVERSE EVENTS IN NEUROSURGERY
Kiyohiro Houkin, Takeo Baba, Yoshihiro Minamida, Tadashi Nonaka, Izumi Koyanagi, Satoshi Iiboshi
NEUROSURGERY, 65, 3, 587, 594, Sep. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Cerebral Ischemia Originating from Rotational Vertebral Artery Occlusion Caused by C5/6 Spondylotic Changes: A Case Report
Misuzu Ohsaka, Masayoshi Takigami, Izumi Koyanagi, Sangnyon Kim, Kiyohiro Houkin
NEUROLOGICAL SURGERY, 37, 8, 797, 802, Aug. 2009, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Spinal cord infarct as the initial clinical presentation of intravascular malignant lymphomatosis
He Liu, Izumi Koyanagi, Hideki Chiba, Masahiro Wanibuchi, Osamu Honmou, Toshiaki Yamaki, Kiyohiro Houkin
JOURNAL OF CLINICAL NEUROSCIENCE, 16, 4, 570, 573, Apr. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Reversible Hyperintense Lesion after Balloon Occlusion Test
Koichi Haraguchi, Kiyohiro Houkin, Izumi Koyanagi, Tadashi Nonaka, Takeo Baba
JOURNAL OF NEUROIMAGING, 19, 2, 174, 178, Apr. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Therapeutic benefits of angiogenetic gene-modified human mesenchymal stem cells after cerebral ischemia
Kentaro Toyama, Osamu Honmou, Kuniaki Harada, Junpei Suzuki, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
EXPERIMENTAL NEUROLOGY, 216, 1, 47, 55, Mar. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Susceptibility-weighted magnetic resonance imaging for the detection of cerebral microhemorrhage in patients with traumatic brain injury.
Akiyama Y, Miyata K, Harada K, Minamida Y, Nonaka T, Koyanagi I, Asai Y, Houkin K
Neurologia medico-chirurgica, 49, 3, 97, 99, 3, Mar. 2009, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The sensitivity of susceptibility-weighted magnetic resonance (MR) imaging was compared with conventional MR sequences, including T(2)*-weighted imaging, and computed tomography for the detection of cerebral hemorrhages in 15 patients with head injury. Susceptibility-weighted imaging detected a mean of 76+/-52 (total 1132) hypointense spotty lesions, compared to a mean of 21+/-19 (total 316) detected by T(2)*-weighted imaging (p<0.0001, paired t-test). Susceptibility-weighted imaging is extremely sensitive for the visualization and detection of microhemorrhages.

Susceptibility-Weighted Magnetic Resonance Imaging for the Detection of Cerebral Microhemorrhage in Patients With Traumatic Brain Injury
Yukinori Akiyama, Kei Miyata, Kuniaki Harada, Yoshihiro Minamida, Tadashi Nonaka, Izumi Koyanagi, Yasufumi Asai, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 49, 3, 97, 99, Mar. 2009, [Peer-reviewed]
English, Scientific journal

ウイリス動脈輪閉塞症における病態・治療に関する研究 3DCT-Angioと3T-MRAによるもやもや病診断について
飯星智史, 寳金清博
ウィリス動脈輪閉塞症における病態・治療に関する研究 平成20年度 総括・分担研究報告書, 2009

Stroke Teamによる,NIHSSとt-PAに関する医学部卒前教育と卒業試験の実施
齊藤正樹, 米増保之, 山本和利, 姉川敬裕, 高橋明, 岡村耕一, 矢坂正弘, 岡田靖, 下濱俊, 寳金清博
日本脳神経外科救急学会プログラム・抄録集, 14th, 2009

後交通動脈と前脈絡叢動脈の起始に関する2症例の検討
飯星智史, 宮田圭, 斉藤正樹, 野中雅, 小柳泉, 寳金清博
日本神経放射線学会プログラム・抄録集, 38th, 2009

Suprasellar Hemangioblastoma
Shiro Miyata, Takeshi Mikami, Yoshihiro Minamida, Yukinori Akiyama, Kiyohiro Houkin
JOURNAL OF NEURO-OPHTHALMOLOGY, 28, 4, 325, 326, Dec. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Moyamoya disease: current concepts and future perspectives
Satoshi Kuroda, Kiyohiro Houkin
LANCET NEUROLOGY, 7, 11, 1056, 1066, Nov. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Current knowledge on the genetic factors involved in moyamoya disease
Hirokuni Hashikata, Wanyang Liu, Yohei Mineharu, Kayoko Inoue, Katsunobu Takenaka, Hidetoshi Ikeda, Kiyohiro Houkin, Satoshi Kuroda, Kenji Kikuchi, Mitsuru Kimura, Takuyuu Taki, Makoto Sonobe, Sadahiko Ban, Hidekazu Nogaki, Akira Handa, Kenichiro Kikuta, Yasushi Takagi, Kazuhiko Nozaki, Nobuo Hashimoto, Akio Koizumi
Brain and Nerve, 60, 11, 1261, 1269, 11, Nov. 2008, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

もやもや病に対するSTA‐MCA anastomosis+EDMAPSの治療成績
黒田敏, 中山若樹, 石川達哉, 宝金清博, 岩崎喜信
脳循環代謝, 20, 1, 153, 153, (一社)日本脳循環代謝学会, Nov. 2008
Japanese

Optimization of a therapeutic protocol for intravenous injection of human mesenchymal stem cells after cerebral ischemia in adult rats
Yoshinori Omori, Osamu Honmou, Kuniaki Harada, Junpei Suzuki, Kiyohiro Houkin, Jeffery D. Kocsis
BRAIN RESEARCH, 1236, 30, 38, Oct. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Interstitial spinal-cord oedema in syringomyelia associated with Chiari type 1 malformations
Y. Akiyama, I. Koyanagi, K. Yoshifuji, T. Murakami, T. Baba, Y. Minamida, T. Nonaka, K. Houkin
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 79, 10, 1153, 1158, Oct. 2008, [Peer-reviewed]
English, Scientific journal

Radiological findings and clinical course of conus lipoma: Implications for surgical treatment
Izumi Koyanagi, Kazutoshi Hida, Yoshinobu Iwasaki, Toyohiko Isu, Masami Yoshino, Tomohiro Murakami, Kazuhisa Yoshifuji, Kiyohiro Houkin
NEUROSURGERY, 63, 3, 546, 551, Sep. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Novel epidemiological features of moyamoya disease
T. Baba, K. Houkin, S. Kuroda
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 79, 8, 900, 904, Aug. 2008, [Peer-reviewed]
English, Scientific journal

Autosomal dominant moyamoya disease maps to chromosome 17q25.3.
Mineharu Y, Liu W, Inoue K, Matsuura N, Inoue S, Takenaka K, Ikeda H, Houkin K, Takagi Y, Kikuta K, Nozaki K, Hashimoto N, Koizumi A
Neurology, 70, 24 Pt 2, 2357, 2363, Jun. 2008, [Peer-reviewed]
English

Evaluation of carotid plaque composition by computed tomographic angiography and black blood magnetic resonance images
K. Haraguchi, K. Houkin, I. Koyanagi, T. Nonaka, T. Baba
MINIMALLY INVASIVE NEUROSURGERY, 51, 2, 91, 94, Apr. 2008, [Peer-reviewed]
English, Scientific journal

Evaluation of Carotid Plaque Composition by Computed Tomography Angiography and Black Blood Magnetic Resonance Image
K. Haraguchi, K. Houkin, I. Koyanagi, T. Nonaka, T. Baba
INTERVENTIONAL NEURORADIOLOGY, 14, 1, 39, 43, Mar. 2008, [Peer-reviewed]
English, Scientific journal

Therapeutic benefits by human mesenchymal stem cells (hMSCs) and Ang-1 gene-modified hMSCs after cerebral ischemia
Toshiyuki Onda, Osamu Honmou, Kuniaki Harada, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 28, 2, 329, 340, Feb. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Moyamoya disease presenting with subarachnoid hemorrhage localized over the frontal cortex: case report.
Toshiya Osanai, Satoshi Kuroda, Naoki Nakayama, Tomohiro Yamauchi, Kiyohiro Houkin, Yoshinobu Iwasaki
Surgical neurology, 69, 2, 197, 200, 2, Feb. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: In moyamoya disease, intracranial bleeding is known to occur because of the rupture of saccular aneurysms in the circle of Willis or because of the rupture of dilated, fragile moyamoya vessels. The former causes subarachnoid hemorrhage (SAH), and the latter causes intracerebral or intraventricular hemorrhage. CASE DESCRIPTION: In this report, we describe the case of a 34-year-old woman with moyamoya disease who suddenly developed headache and jacksonian seizure. Plain computed tomographic scans on admission revealed SAH localized over the left frontal cortex. The patient was diagnosed with moyamoya disease on cerebral angiography. However, no aneurysm was found on cerebral angiography. Positron emission tomography showed the reduction of CBF and its reactivity to acetazolamide and the elevation of CBV in the left hemisphere. She underwent STA to MCA anastomosis and indirect synangiosis. Intraoperative observations revealed that the pial arterioles were markedly dilated on the brain surface. The CBF in the left hemisphere significantly improved after surgery. The patient has experienced no further episode of cerebral ischemia or intracranial bleeding. CONCLUSIONS: Subarachnoid hemorrhage of unknown cause is quite rare in moyamoya disease. Based on the findings in the present case, the dilated collateral arteries on the brain surface may rupture and cause SAH over the cerebral cortex, which is the third cause of intracranial bleeding in patients with persistent cerebral ischemia due to moyamoya disease.

Autosomal dominant moyamoya disease maps to chromosome 17q25.3. Neurology. 2008 Jun 10;70(24 Pt 2):2357-63. Epub 2008 May 7.　　　　　　　　　　　　　　　
Mineharu Y, Liu W, Inoue K, Matsuura N, Inoue S, Takenaka K, Ikeda H, Houkin K, Takagi Y, Kikuta K, Nozaki K, Hashimoto N, Koizumi A
2008, [Peer-reviewed]
English

Clinical manifestations and surgical results for paraclinoid cerebral aneurysms presenting with visual symptoms
Tadashi Nonaka, Koichi Haraguchi, Takeo Baba, Izumi Koyanagi, Kiyohiro Houkin
SURGICAL NEUROLOGY, 67, 6, 612, 619, Jun. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Mesenchymal stem cells derived from peripheral blood protects against ischemia
Ryo Ukai, Osamu Honmou, Kuniaki Harada, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
JOURNAL OF NEUROTRAUMA, 24, 3, 508, 520, Mar. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Transorbital intracranial penetrating injury from impaling on an earpick
Taro Yamashita, Takeshi Mikami, Takeo Baba, Yoshihiro Minamida, Toshiya Sugino, Izumi Koyanagi, Tadashi Nonaka, Kiyohiro Houkin
JOURNAL OF NEURO-OPHTHALMOLOGY, 27, 1, 48, 49, Mar. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

[Surgical site infections in neurosurgery: perioperative management].
Minamida Y, Houkin K
Nihon rinsho. Japanese journal of clinical medicine, 65 Suppl 3, 573, 578, Mar. 2007, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Magnetic resonance lactate and lipid signals in rat brain after middle cerebral artery occlusion model
Kuniaki Harada, Osamu Honmou, He Liu, Michio Bando, Kiyohiro Houkin, Jeffery D. Kocsis
BRAIN RESEARCH, 1134, 1, 206, 213, Feb. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Free Flap Transfer for the Treatment of Intractable Postcraniotomy Subdural Empyemas and Epidural Abscesses
Takeshi Mikami, Yoshihiro Minamida, Toshiya Sugino, Izumi Koyanagi, Takatoshi Yotsuyanagi, Kiyohiro Houkin
Operative Neurosurgery, 60, suppl_2, ONS, 83, 01 Feb. 2007, [Peer-reviewed]
Scientific journal

NOVEL BIPOLAR FORCEPS WITH PROTEIN REPELLENCE USING GOLD–POLYTETRAFLUOROETHYLENE COMPOSITE FILM
Takeshi Mikami, Yoshihiro Minamida, Izumi Koyanagi, Kiyohiro Houkin
Operative Neurosurgery, 60, 157, 161, Feb. 2007, [Peer-reviewed]
Scientific journal

Delayed thromboembolic infarction associated with reconfiguration of guglielmi detachable coils - Case report -
Koichi Haraguchi, Kiyohiro Houkin, Tadashi Nonaka, Takeo Baba
NEUROLOGIA MEDICO-CHIRURGICA, 47, 2, 79, 82, Feb. 2007, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Free flap transfer for the treatment of intractable postcraniotomy subdural empyemas and epidural abscesses
Takeshi Mikami, Yoshihiro Minamida, Toshiya Sugino, Izumi Koyanagi, Takatoshi Yotsuyanagi, Kiyohiro Houkin
NEUROSURGERY, 60, 2, 83, 87, Feb. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Novel bipolar forceps with protein repellence using gold-polytetrafluoroethylene composite film
Takeshi Mikami, Yoshihiro Minamida, Izumi Koyanagi, Kiyohiro Houkin
NEUROSURGERY, 60, 2, 157, 160, Feb. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Moyamoya disease and pregnancy: A prospective study
Kuroda Satoshi, Ishikawa Tatsuya, Nakayama Naoki, Houkin Kiyohiro, Iwasaki Yoshinobu
STROKE, 38, 2, 525, 526, Feb. 2007, [Peer-reviewed]

Alveolar soft part sarcoma in brain with cardiac metastasis. A case report
Yukinori Akiyama, Takeo Baba, Yukihiro Ibayashi, Yasufumi Asai, Kiyohiro Houkin
International Journal of Cardiology, 114, 3, E93, E95, 3, 18 Jan. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Anatomical variations in pneumatization of the anterior clinoid process
Takesm Mikami, Yoshimro Minamma, Immi Koyanagi, Takeo Baba, Kiyomro Houkin
JOURNAL OF NEUROSURGERY, 106, 1, 170, 174, Jan. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

脳梗塞に対する骨髄幹細胞治療
本望修, 寳金清博
脳卒中, 29, 2, 2007

Low Grade Gliomaの治療方針
秋山幸功, 南田善弘, 小柳泉, 野中雅, 寳金清博
日本脳神経外科学会総会抄録集(CD-ROM), 66th, 2007

Neural differentiation potential of peripheral blood- and bone-marrow-derived precursor cells
Sangnyon Kim, Osamu Honmou, Kazunori Kato, Tadashi Nonaka, Kiyohiro Houkin, Hirufumi Amada, Jeffery D. Kocsis
BRAIN RESEARCH, 1123, 1, 27, 33, Dec. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

[Stereotactic removal and thrombolysis of intracerebral hematoma].
Haraguchi K, Houkin K
Nihon rinsho. Japanese journal of clinical medicine, 64 Suppl 8, 361, 363, Nov. 2006, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Intravenous administration of glial cell line-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in the adult rat
Yoshifumi Horita, Osamu Honmou, Kuniaki Harada, Kiyohiro Houkin, Hirofumi Hamada, Jeffery D. Kocsis
JOURNAL OF NEUROSCIENCE RESEARCH, 84, 7, 1495, 1504, Nov. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

Optimization of three-dimensional time-of-flight magnetic resonance angiography of the intracranial arteries
Kuniaki Harada, Osamu Honmou, Yoshihiro Odawara, Michio Bando, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 46, 11, 523, 528, Nov. 2006, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Marked saggital sinus dilatation and thrombi without thrombosis in a patient with systemic lupus erythematosus
Susumu Chiba, Takeshi Koizumi, Hiroyuki Matsumoto, Misuzu Ohsaka, Tadashi Nonaka, Kiyohiro Houkin
CLINICAL RHEUMATOLOGY, 25, 6, 911, 913, Nov. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

[A cell therapy for stroke].
Honmou O, Houkin K
Nihon rinsho. Japanese journal of clinical medicine, 64 Suppl 7, 664, 666, Oct. 2006, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

False-negative cerebral infarction on diffusion magnetic resonance imaging
Yukinori Akiyama, Yasufumi Asai, Kiyohiro Houkin
AMERICAN JOURNAL OF EMERGENCY MEDICINE, 24, 6, 746, 748, Oct. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

Neuroprotection by PIGF gene-modified human mesenchymal stem cells after cerebral ischaemia
H. Liu, Osamu Honmou, K. Harada, K. Nakamura, K. Houkin, H. Hamada, J. D. Kocsis
Brain, 129, 10, 2734, 2745, Oct. 2006, [Peer-reviewed]
English, Scientific journal

Lhermitte-Duclos disease with cervical paraspinal arteriovenous fistula - Case report
Yukinori Akiyama, Jun Ikeda, Yukihiro Ibayashi, Tadashi Nonaka, Yasufumi Asai, Kiyohiro Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 46, 9, 446, 449, Sep. 2006, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Cerebrospinal fluid leak due to inner ear malformations
Takeshi Mikami, Yoshihiro Minamida, Tetsuo Himi, Kiyohiro Houkin
NEUROLOGICAL SURGERY, 34, 9, 933, 937, Sep. 2006, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Primary intraosseous cavernous hemangioma in anterior clinoid process
Taro Yamashita, Takeshi Mikami, Yoshihiro Minamida, Takeo Baba, Kiyohiro Houkin
NEUROLOGICAL SURGERY, 34, 8, 833, 837, Aug. 2006, [Domestic magazines]
Japanese, Scientific journal

STA‐MCAバイパス術後のhyperperfusion:発生状況とその発生因子の検討
中山若樹, 黒田敏, 石川達哉, 志賀哲, 宝金清博, 玉木長良, 岩崎喜信
脳循環代謝, 18, 2, 135, 135, (一社)日本脳循環代謝学会, Aug. 2006
Japanese

Intravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
T. Honma, O. Honmou, S. Iihoshi, K. Harada, K. Houkin, H. Hamada, J. D. Kocsis
EXPERIMENTAL NEUROLOGY, 199, 1, 56, 66, May 2006, [Peer-reviewed]
English, Scientific journal

Clinical features of familial moyamoya disease
R Nanba, S Kuroda, M Tada, T Ishikawa, K Houkin, Y Iwasaki
CHILDS NERVOUS SYSTEM, 22, 3, 258, 262, Mar. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

A case of moyamoya disease presenting with chorea
W Zheng, M Wanibuchi, T Onda, H Liu, Koyanagi, I, K Fujimori, K Houkin
CHILDS NERVOUS SYSTEM, 22, 3, 274, 278, Mar. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

Cerebral oxygen metabolism and neuronal integrity in patients with impaired vasoreactivity attributable to occlusive carotid artery disease
S Kuroda, T Shiga, K Houkin, T Ishikawa, C Katoh, N Tamaki, Y Iwasaki
STROKE, 37, 2, 393, 398, Feb. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

Acute necrotizing encephaloplathy associated with human herpesvirus-6 infection
M Ohsaka, K Houkin, M Takigami, Koyanagi, I
PEDIATRIC NEUROLOGY, 34, 2, 160, 163, Feb. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

Neuronal cell injury in patients after cardiopulmonary resuscitation: evaluation by diffusion-weighted imaging and magnetic resonance spectroscopy
H Kano, K Houkin, K Harada, Koyanagi, I, S Nara, Y Itou, H Imaizumi, Y Asai, M Saitou
NEUROSURGICAL REVIEW, 29, 1, 88, 92, Jan. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

鼻内視鏡手術における未固定凍結遺体を用いたトレーニングシステム
南田善弘, 三上毅, 寳金清博, 村上弦
日本内分泌学会雑誌, 82, Suppl. (June), 2006

脳血管障害総論 XI.治療動向 開発・研究中の遺伝子治療,再生医療 各論 脳梗塞の細胞療法(神経再生治療)-自家骨髄細胞の静脈内投与-
本望修, 寳金清博
日本臨床, 64, 2006

Superior sagittal sinus thrombosis in a case of longstanding systemic lupus erythematosus
Misuzu Ohsaka, Tadashi Nonaka, Shinichi Oka, Yoshihiro Minamida, Takeshi Mikami, Kiyohiro Houkin
Brain and Nerve, 58, 1, 57, 61, Jan. 2006, [Peer-reviewed]
Japanese, Scientific journal

Risk factors of postprocedural hypotension following carotid artery stenting
T Nonaka, S Oka, S Miyata, T Baba, T Mikami, K Houkin
INTERVENTIONAL NEURORADIOLOGY, 12, 205, 210, Jan. 2006, [Peer-reviewed]
English, Scientific journal

The 5-hydroxytryptamine2A receptor antagonist sarpogrelate hydrochloride inhibits acute platelet aggregation in injured endothelium
K. Houkin, N. Nakayama, T. Nonaka, I. Koyanagi
Journal of International Medical Research, 34, 1, 65, 72, Cambridge Medical Publications, 2006, [Peer-reviewed]
English, Scientific journal

Entrapment neuropathy of the optic nerve due to hyperostosis associated with congenital anemia - Case report
T Baba, Y Minamida, T Mikami, Koyanagi, I, K Houkin
JOURNAL OF NEUROSURGERY, 103, 5, 917, 919, Nov. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Incidence and clinical features of disease progression in adult moyamoya disease
S Kuroda, T Ishikawa, K Houkin, R Nanba, M Hokari, Y Iwasaki
STROKE, 36, 10, 2148, 2153, Oct. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Cranial nerve assessment in posterior fossa tumors with fast imaging employing steady-state acquisition (FIESTA)
T Mikami, Y Minamida, T Yamaki, Koyanagi, I, T Nonaka, K Houkin
NEUROSURGICAL REVIEW, 28, 4, 261, 266, Oct. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Inadequate website disclosure of surgical outcome of intracranial aneurysms - Survey of 1225 sites in Japan
K Houkin, T Nonaka, S Oka, Koyanagi, I
NEUROLOGIA MEDICO-CHIRURGICA, 45, 9, 448, 453, Sep. 2005, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Acquired factor VIII inhibitors in a patient associated with neurosurgery: Case report and review of the literature
T Mikami, N Masauzi, J Niwa, K Houkin
NEUROSURGERY, 57, 3, 595, 595, Sep. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Prediction of prolonged postprocedural hypotension after carotid artery stenting
Tadashi Nonaka, Shinichi Oka, Kei Miyata, Takeshi Mikami, Izumi Koyanagi, Kiyohiro Houkin, Kazuhisa Yoshifuji, Toshio Imaizumi
Neurosurgery, 57, 3, 472, 476, 3, Sep. 2005, [Peer-reviewed]
English, Scientific journal

Prediction of prolonged postprocedural hypotension after carotid artery stenting
T Nonaka, S Oka, K Miyata, T Mikami, L Koyanagi, K Houkin, K Yoshifuji, T Imaizumi
NEUROSURGERY, 57, 3, 472, 476, Sep. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Metastatic renal cell carcinoma mimicking pituitary adenoma - Case report
H Liu, T Yamaki, S Oka, Koyanagi, I, K Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 45, 8, 418, 422, Aug. 2005, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Surgical management of the recurrence and regrowth of craniopharyngiomas
Y Minamida, T Mikami, K Hashi, K Houkin
JOURNAL OF NEUROSURGERY, 103, 2, 224, 232, Aug. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Clinical features and pathomechanisms of syringomyelia associated with spinal arachnoiditis
Koyanagi, I, Y Iwasaki, K Hida, K Houkin
SURGICAL NEUROLOGY, 63, 4, 350, 355, Apr. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Carotid rete mirabile associated with subarachnoid hemorrhage - Case report
T Mikami, A Takahashi, K Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 45, 4, 201, 204, Apr. 2005, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

BDNF産生ヒト間葉系幹細胞の経静脈的移植による脳梗塞治療の検討
野村 達史, 本望 修, 原田 邦明, 劉 赫, 寶金 清博, 中村 公則, 濱田 洋文
脳卒中, 27, 1, 203, 203, (一社)日本脳卒中学会, Apr. 2005
Japanese

Mesenchymal stem cells (MSC) as therapeutic cytoreagents for gene therapy
H Hamada, M Kobune, K Nakamura, Y Kawano, K Kato, O Honmou, K Houkin, T Matsunaga, YN Niitsu
CANCER SCIENCE, 96, 3, 149, 156, Mar. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Novel stent/CEA strategy for patients with bilateral carotid artery stenosis and coronary artery disease: Report of 2 cases
H Shichinohe, S Kuroda, T Asano, S Ushikoshi, T Ishikawa, K Houkin, T Murashita, Y Iwasaki
NEUROLOGICAL SURGERY, 33, 2, 149, 153, Feb. 2005, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Novel magnetic resonance angiography stage grading for Moyamoya disease
K Houkin, N Nakayama, S Kuroda, T Nonaka, T Shonai, T Yoshimoto
CEREBROVASCULAR DISEASES, 20, 5, 347, 354, 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Mesenchymal stem cells that produce neurotrophic factors reduce ischemic damage in the rat middle cerebral artery occlusion model
K Kurozumi, K Nakamura, T Tamiya, Y Kawano, K Ishii, M Kobune, S Hirai, H Uchida, K Sasaki, Y Ito, K Kato, O Honmou, K Houkin, Date, I, H Hamada
MOLECULAR THERAPY, 11, 1, 96, 104, Jan. 2005, [Peer-reviewed]
English, Scientific journal

Combined surgical and endovascular treatment of infected pseudoaneurysm after carotid endarterectomy - Case report
H Yasuda, S Kuroda, S Ushikoshi, T Kato, K Houkin, Y Iwasaki, H Abe
NEUROLOGIA MEDICO-CHIRURGICA, 45, 1, 37, 40, Jan. 2005, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Sequence analysis and bioinformatics analysis of chromosome 17q25 in familial moyamoya disease
R Nanba, M Tada, S Kuroda, K Houkin, Y Iwasaki
CHILDS NERVOUS SYSTEM, 21, 1, 62, 68, Jan. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

I.v. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
T Nomura, O Honmou, K Harada, K Houkin, H Hamada, JD Kocsis
NEUROSCIENCE, 136, 1, 161, 169, 2005, [Peer-reviewed]
English, Scientific journal

Increased expression of hepatocyte growth factor in cerebrospinal fluid and intracranial artery in moyamoya disease
R Nanba, S Kuroda, T Ishikawa, K Houkin, Y Iwasaki
STROKE, 35, 12, 2837, 2842, Dec. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Autologous transplantation of expanded neural precursor cells into the demyelinated monkey spinal cord
S Oka, O Honmou, Y Akiyama, M Sasaki, K Houkin, K Hashi, JD Kocsis
BRAIN RESEARCH, 1030, 1, 94, 102, Dec. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Stereotactic irradiation for intracranial arteriovenous malformation using stereotactic radiosurgery or hypofractionated stereotactic radiotherapy
TC Chang, H Shirato, H Aoyama, S Ushikoshi, N Kato, S Kuroda, T Ishikawa, K Houkin, Y Iwasaki, K Miyasaka
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 60, 3, 861, 870, Nov. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Spinal canal size in ossification of the posterior longitudinal ligament of the cervical spine
Koyanagi, I, H Imamura, S Fujimoto, K Hida, Y Iwasaki, K Houkin, DJ Maiman
SURGICAL NEUROLOGY, 62, 4, 286, 291, Oct. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

How does angiogenesis develop in pediatric moyamoya disease after surgery? A prospective study with MR angiography
K Houkin, N Nakayama, S Kuroda, T Ishikawa, T Nonaka
CHILDS NERVOUS SYSTEM, 20, 10, 734, 741, Oct. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Superior oblique myokymia caused by vascular compression
M Hashimoto, K Ohtsuka, Y Suzuki, Y Minamida, K Houkin
JOURNAL OF NEURO-OPHTHALMOLOGY, 24, 3, 237, 239, Sep. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

In vivo quantification of the metabolites in normal brain and brain tumors by proton MR spectroscopy using water as an internal standard
ZY Tong, T Yamaki, K Harada, K Houkin
MAGNETIC RESONANCE IMAGING, 22, 7, 1017, 1024, Sep. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Diacylglycerol kinase gamma serves as an upstream suppressor of Rac1 and lamellipodium formation
S Tsushima, M Kai, K Yamada, S Imai, K Houkin, H Kanoh, F Sakane
JOURNAL OF BIOLOGICAL CHEMISTRY, 279, 27, 28603, 28613, Jul. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Consequence of intellectual property and consortium project in neurosurgery
Osamu Honmou, Kiyohiro Houkin
Neurological Surgery, 32, 6, 571, 577, 6, Jun. 2004, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

[Surgical treatment for cerebral aneurysm after incomplete Guglielmi detachable coil embolization].
Satoshi Kuroda, Satoshi Ushikoshi, Yoshimasa Niiya, Toshiya Osanai, Tatsuya Ishikawa, Shugo Takikawa, Kiyohiro Houkin, Yoshinobu Iwasaki
No shinkei geka. Neurological surgery, 32, 6, 645, 50, 6, Jun. 2004, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, The frequency of surgical treatment for intracranial aneurysms that have been incompletely treated by endovascular coiling will increase in time. The authors describe their experience in the following 3 patients. There was one non-ruptured aneurysm and two ruptured ones. The intervals between coiling and surgery were 1, 5, and 10 months. Surgery was indicated because of partial treatment and/or growth of residual neck. Observation during surgery revealed that coils were exposed to the subarachnoid space at the tip of the aneurysm in the cases of ruptured aneurysms. When the residual neck was large enough for the size of the clip blades, there was no need to remove the coils. The coils in the neck were able to be removed when the interval between coiling and surgery was very short, but could not be removed when the interval was prolonged. Intraoperative angiography and reconstructive bypass surgery may be essential in these complex cases. As pointed out previously, the operative approach should be determined by the need for coil removal and the duration since coiling.

Reduced blood flow and preserved vasoreactivity characterize oxygen hypometabolism due to incomplete infarction in occlusive carotid artery diseases
S Kuroda, T Shiga, T Ishikawa, K Houkin, T Narita, C Katoh, N Tamaki, Y Iwasaki
JOURNAL OF NUCLEAR MEDICINE, 45, 6, 943, 949, Jun. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Gradual recovery from dyslexia and related serial magnetoencephalographic changes in the lexicosemantic centers after resection of a mesial temporal astrocytoma - Case report
K Kamada, Y Sawamura, F Takeuchi, K Houkin, H Kawaguchi, Y Iwasaki, S Kuriki
JOURNAL OF NEUROSURGERY, 100, 6, 1101, 1106, Jun. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

In vivo quantification of the metabolites in normal brain and brain tumors by proton MR spectroscopy using water as an internal standard
ZY Tong, T Yamaki, K Harada, K Houkin
MAGNETIC RESONANCE IMAGING, 22, 5, 735, 742, Jun. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Determinants of intellectual outcome after surgical revascularization in pediatric moyamoya disease: a multivariate analysis
S Kuroda, K Houkin, T Ishikawa, N Nakayama, J Ikeda, N Ishii, H Kamiyama, Y Iwasaki
CHILDS NERVOUS SYSTEM, 20, 5, 302, 308, May 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

A therapeutic window for intravenous administration of autologous bone marrow after cerebral ischemia in adult rats
S Iihoshi, O Honmou, K Houkin, K Hashi, JD Kocsis
BRAIN RESEARCH, 1007, 1-2, 1, 9, May 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

ラット脳梗塞モデルに対する経静脈的骨髄幹細胞移植療法
本間 敏美, 本望 修, 原田 邦明, 備前 明子, 劉 赫, 野村 達史, 中村 公則, 濱田 洋文, 寶金 清博
脳卒中, 26, 1, 185, 185, (一社)日本脳卒中学会, Mar. 2004
Japanese

Pitfalls in cerebral revascularization surgery
Kiyohiro Houkin
International Congress Series, 1259, C, 313, 320, 01 Feb. 2004, [Peer-reviewed]
English, Scientific journal

Familial moyamoya disease - Clinical features and current study
R Nanba, S Kuroda, T Ishikawa, Y Iwasaki, M Tada, H Kiyohiro
NEUROLOGICAL SURGERY, 32, 1, 7, 16, Jan. 2004, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Source localization of the re-build up phenomenon in pediatric moyamoya disease - a dipole distribution analysis using MEG and SPECT
F Qiao, S Kuroda, K Kamada, K Houkin, Y Iwasaki
CHILDS NERVOUS SYSTEM, 19, 10-11, 760, 764, Nov. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Evaluation of blood flow in carotid artery stenosis using B-flow sonography
T Mikami, A Takahashi, K Houkin
NEUROLOGIA MEDICO-CHIRURGICA, 43, 11, 528, 532, Nov. 2003, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Identification and Characterization of Two Splice Variants of Human Diacylglycerol Kinase η
Tomohiro Murakami, Fumio Sakane, Shin-Ichi Imai, Kiyohiro Houkin, Hideo Kanoh
Journal of Biological Chemistry, 278, 36, 34364, 34372, 36, 05 Sep. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Histone H2AX sensitizes glioma cells to genotoxic stimuli by recruiting DNA double-strand break repair proteins
H Momota, S Ichimiya, N Kondo, T Kikuchi, T Torigoe, T Yamaki, K Houkin, N Sato
INTERNATIONAL JOURNAL OF ONCOLOGY, 23, 2, 311, 315, Aug. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Telomerized human multipotent mesenchymal cells can differentiate into hematopoietic and cobblestone area-supporting cells
M Kobune, Y Kawano, Y Ito, H Chiba, K Nakamura, H Tsuda, K Sasaki, H Dehari, H Uchida, O Honmou, S Takahashi, A Bizen, R Takimoto, T Matsunaga, J Kato, K Kato, K Houkin, Y Niitsu, H Hamada
EXPERIMENTAL HEMATOLOGY, 31, 8, 715, 722, Aug. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

間葉系幹細胞(MSC)を用いた浸潤性グリオーマ遺伝子治療の開発　　　　　　　　　　　　　　　
中村 公則, 伊藤 克礼, 河野 豊, 黒住 和彦, 佐々木 勝則, 備前 明子, 本望 修, 宝金 清博, 濱田 洋文
日本癌学会総会記事, 62回, 325, 325, 日本癌学会, Aug. 2003
Japanese

A case of sinonasal carcinoma with intracranial invasion treated by a multidisciplinary team
S Kin, M Wanibuschi, Y Minamida, T Yamaki, S Tanabe, K Houkin
NEUROLOGICAL SURGERY, 31, 6, 677, 682, Jun. 2003, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Visualization of the eloquent motor system by integration of Meg, functional, and anisotropic diffusion-weighted MRI in functional neuronavigation
K Kamada, K Houkin, F Takeuchi, N Ishii, J Ikeda, Y Sawamura, S Kuriki, H Kawaguchi, Y Iwasaki
SURGICAL NEUROLOGY, 59, 5, 353, 362, May 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Subclavian steal syndrome associated with the right aortic arch in a patient with Peutz-Jeghers syndrome: Case report
M Hokari, S Kuroda, K Furukawa, K Houkin, Y Iwasaki
NEUROLOGICAL SURGERY, 31, 3, 281, 286, Mar. 2003, [Domestic magazines]
Japanese, Scientific journal

Role of Chlamydia pneumoniae-infected macrophages in atherosclerosis developments of the carotid artery
S Kuroda, T Kobayashi, N Ishii, J Ikeda, Y Shinohe, K Houkin, Y Iwasaki, K Nagashima
NEUROPATHOLOGY, 23, 1, 1, 8, Mar. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Calcium-induced mitochondrial swelling and cytochrome c release in the brain: its biochemical characteristics and implication in ischemic neuronal injury
T Kobayashi, S Kuroda, M Tada, K Houkin, Y Iwasaki, H Abe
BRAIN RESEARCH, 960, 1-2, 62, 70, Jan. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Transplantation of mesenchymal stem cells derived from the bone marrow into the demyelinated spinal cord
A Bizen, O Honmou, M Inoue, S Iihoshi, K Houkin, K Hashi
MOLECULAR MECHANISMS AND EPOCHAL THERAPEUTICS OF ISCHEMIC STROKE AND DEMENTIA, 1252, 471, 475, 2003, [Peer-reviewed]
English, International conference proceedings

Clinical significance of posterior cerebral artery stenosis/occlusion in moyamoya disease
S Kuroda, T Ishikawa, K Houkin, Y Iwasaki
NEUROLOGICAL SURGERY, 30, 12, 1295, 1300, Dec. 2002, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Three cases of successful stenting for radiation-induced carotid arterial stenosis
J Kitamura, S Kuroda, S Ushikoshi, K Furukawa, T Asano, H Shichinohe, K Houkin, Y Iwasaki, H Saitoh, K Mitsumori
NEUROLOGICAL SURGERY, 30, 10, 1097, 1102, Oct. 2002, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal

Rapid identification of the primary motor area by using magnetic resonance axonography
K Kamada, K Houkin, Y Iwasaki, F Takeuchi, S Kuriki, K Mitsumori, Y Sawamura
JOURNAL OF NEUROSURGERY, 97, 3, 558, 567, Sep. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

Surgical treatment of intracranial dural arteriovenous fistulas
S Ushikoshi, K Houkin, S Kuroda, T Asano, Y Iwasaki, K Miyasaka, H Abe
SURGICAL NEUROLOGY, 57, 4, 253, 261, Apr. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

Angioplasty with stenting for intracranial vertebral and basilar artery stenosis: Three technical case reports and literature review
S Yano, K Houkin, S Abe, H Saitoh, S Kuroda, T Asano, S Ushikoshi, Y Iwasaki
NEUROLOGICAL SURGERY, 29, 11, 1081, 1090, Nov. 2001, [Peer-reviewed]
Japanese, Scientific journal

Effects of surgical revascularization on peripheral artery aneurysms in Moyamoya disease: Report of three cases
S Kuroda, K Houkin, H Kamiyama, H Abe
NEUROSURGERY, 49, 2, 463, 467, Aug. 2001, [Peer-reviewed]
English, Scientific journal

もやもや病に対する脳血行再建術と長期予後
黒田敏, 宝金清博, 中山若樹, 成田拓人, 上山博康, 岩崎喜信
小児の脳神経, 26, 4, 325, 325, (一社)日本小児神経外科学会, Aug. 2001
Japanese

もやもや病における血行再建術後の血行動態変化 MRAを用いた前向き調査による検討
中山若樹, 宝金清博, 黒田敏, 岩崎喜信
小児の脳神経, 26, 4, 271, 271, (一社)日本小児神経外科学会, Aug. 2001
Japanese

脳梗塞における拡散テンソルの経時的変化
中山若樹, 宝金清博, 斎藤久寿
脳循環代謝, 13, 2, 106, 107, (一社)日本脳循環代謝学会, Jul. 2001
Japanese

小児モヤモヤ病における知的機能障害 WISC‐R下位項目別の障害度と脳血流障害との関連
中山若樹, 黒田敏, 宝金清博, 斎藤伸治, 岩崎喜信, 阿部弘
脳卒中, 23, 1, 90, 90, (一社)日本脳卒中学会, Mar. 2001
Japanese

Frontal lobe infarction due to hemodynamic change after surgical revascularization in moyamoya disease - Two case reports
S Kuroda, K Houkin, M Nunomura, H Abe
NEUROLOGIA MEDICO-CHIRURGICA, 40, 6, 315, 320, Jun. 2000, [Peer-reviewed]
English, Scientific journal

Neovascularization (angiogenesis) after revascularization in Moyamoya disease. Which technique is most useful for Moyamoya disease?
K Houkin, S Kuroda, T Ishikawa, H Abe
ACTA NEUROCHIRURGICA, 142, 3, 269, 276, 2000, [Peer-reviewed]
English, Scientific journal

Aggravation of Brainstem Symptoms Caused by a Large Superior Cerebellar Artery Aneurysm after Embolization by Guglielmi Detachable Coils: Case Report
Satoshi Ushikoshi, Yoichi Kikuchi, Kiyohiro Houkin, Kazuo Miyasaka, Hiroshi Abe
Neurologia Medico-Chirurgica, 39, 7, 524, 529, 1999, [Peer-reviewed]
English, Scientific journal

Intra-operative premature rupture of the cerebral aneurysms. Analysis of the causes and management
K Houkin, S Kuroda, A Takahashi, S Takikawa, T Ishikawa, T Yoshimoto, K Itamato
ACTA NEUROCHIRURGICA, 141, 12, 1255, 1263, 1999, [Peer-reviewed]
English, Scientific journal

Vascular reconstruction using interposed small vessels
K Houkin, T Ishikawa, S Kuroda, H Abe
NEUROSURGERY, 43, 3, 501, 505, Sep. 1998, [Peer-reviewed]
English, Scientific journal

Effectiveness of fibrin glue for preventing postoperative extradural fluid leakage
Tetsuyuki Yoshimoto, Yutaka Sawamura, Kiyohiro Houkin, Hiroshi Abe
Neurologia Medico-Chirurgica, 37, 12, 886, 890, Dec. 1997, [Peer-reviewed]
English, Scientific journal

Childhood ischemic stroke caused by fibromuscular dysplasia of the intracranial artery -Case report-
Adnan Abd Rahman Zurin, Kiyohiro Houkin, Tsuyoshi Asano, Tatsuya Ishikawa, Hiroshi Abe
Neurologia Medico-Chirurgica, 37, 7, 542, 545, Jul. 1997, [Peer-reviewed]
English, Scientific journal

Differentiation of cerebral radiation necrosis from tumor recurrence by proton magnetic resonance spectroscopy
Kyousuke Kamada, Kiyohiro Houkin, Hiroshi Abe, Yutaka Sawamura, Takeshi Kashiwaba
Neurologia Medico-Chirurgica, 37, 3, 250, 256, Mar. 1997, [Peer-reviewed]
English, Scientific journal

Pathological consideration of a ''blister-like'' aneurysm at the superior wall of the internal carotid artery: Case report
T Ishikawa, N Nakamura, K Houkin, M Nomura
NEUROSURGERY, 40, 2, 403, 405, Feb. 1997, [Peer-reviewed]
English, Scientific journal

Angiogenic factors in moyamoya disease
T Yoshimoto, K Houkin, A Takahashi, H Abe
STROKE, 27, 12, 2160, 2165, Dec. 1996, [Peer-reviewed]
English, Scientific journal

Dissociation of vasoreactivity to acetazolamide and hypercapnia - Comparative study in patients with chronic occlusive major cerebral artery disease
K Kazumata, N Tanaka, T Ishikawa, S Kuroda, K Houkin, K Mitsumori
STROKE, 27, 11, 2052, 2058, Nov. 1996, [Peer-reviewed]
English, Scientific journal

Cerebral hypoxia after hyperventilation causes ''re-build-up'' phenomenon and TIA in childhood moyamoya disease - A near-infrared spectroscopy study
S Kuroda, K Houkin, Y Hoshi, M Tamura, K Kazumata, H Abe
CHILDS NERVOUS SYSTEM, 12, 8, 448, 452, Aug. 1996, [Peer-reviewed]
English, Scientific journal

Transient ipsilateral blood flow reduction in the thalamus and cerebral cortex after pontine infarction
S Kuroda, K Houkin, H Abe, K Mitsumori
NEURORADIOLOGY, 38, 3, 239, 242, Apr. 1996, [Peer-reviewed]
English, Scientific journal

Magnetic resonance imaging of cold injury-induced brain edema in rats
Kiyohiro Houkin, Hiroshi Abe, Yuji Hashiguchi, Shigemi Seri
Neurologia Medico-Chirurgica, 36, 2, 72, 77, Feb. 1996, [Peer-reviewed]
English, Scientific journal

Proton MR spectroscopy in patients with unilateral temporal lobe epilepsy
S. Kawaguchi, K. Kamada, K. Itamoto, H. Ohtuki, K. Isonishi, N. Nakayama, M. Shimoyama, K. Tokuda, M. Koiwa, T. Kashiwaba, K. Houkin
Brain and Nerve, 47, 1, 43, 48, 1995, [Peer-reviewed]
Japanese, Scientific journal

Proton MR Spectroscopy in Patients with Unilateral Temporal Lobe Epilepsy.
KAWAGUCHI SUSUMU, KAMADA KYOSUKE, ITAMOTO KOJI, OTSUKI HIROYUKI, ISONISHI KATSUYOSHI, NAKAYAMA NAOKI, SHIMOYAMA MITSUO, TOKUDA KOICHI, HOKIN KIYOHIRO
Brain Nerve, 47, 1, 43-48, Jan. 1995
Japanese

A HIGH-FIELD MAGNETIC-RESONANCE-IMAGING STUDY OF EXPERIMENTAL VASOGENIC BRAIN EDEMA AND ITS RESPONSE TO AVS - 1,2-BIS(NICOTINAMIDO)-PROPANE
K KAMADA, K HOUKIN, Y IWASAKI, H ABE
BRAIN EDEMA IX, 60, 491, 493, 1994, [Peer-reviewed]
English, International conference proceedings

Anatomical analysis of the sources of somatosensory evoked fields (SEFs) using 3D-MRI
K. Kamada, O. Oshiro, F. Takeuchi, S. Kuriki, K. Houkin, H. Abe
Brain and Nerve, 44, 3, 265, 270, 1992, [Peer-reviewed]
Japanese, Scientific journal

REBOUND ALKALOSIS AND PERSISTENT LACTATE - MULTINUCLEAR (H-1, C-13, P-31) NMR SPECTROSCOPIC STUDIES IN RATS
T NAKADA, K HOUKIN, K HIDA, IL KWEE
MAGNETIC RESONANCE IN MEDICINE, 18, 1, 9, 14, Mar. 1991, [Peer-reviewed]
English, Scientific journal

Temporary Occlusion of Parent Artery at Internal Carotid Aneurysm Surgery:-Significance of Exposure of Common Carotid Artery at the Neck of Patient-
NAKAGAWA Yoku, OHSATO Takao, NUNOMURA Mitsuru, IBARA Hiroshi, TADA Mitsuhiro, KOBAYASHI Nobumitsu, HOUKIN Kiyohiro, ISU Toyohiko
Surgery for Cerebral Stroke, 19, 1, 143, 147, The Japanese Society on Surgery for Cerebral Stroke, 1991
Japanese, Temporary clipping of the parent artery in acute-stage aneurysm surgery was popularized by the late Prof. Jiro Suzuki. However, it is not rare that the internal carotid artery (ICA) proximal to the aneurysm cannot easily be identified even during surgery for ICA aneurysms of the common portion. In addition, the difficulty of identification of the ICA proximal to the aneurysm is not always predicted before the operation.
In this paper, authors emphasize that exposure of the common carotid artery in the neck of patients is highly recommended even in aneurysm surgery of the ICA (excluding the cavernous or opthalmic portion) in the cases shown below;
(1) when a ruptured aneurysm protrude posterioly, is located dorsally, or shows up small or large on the angiogram.
(2) when it is not possible to separate the aneurysm from the posterior communicating or anterior choroidal artery on the angiogram.
(3) when younger neurosurgeons perform the operation.

Report on a case of cranial metastasis of thyroid carcinoma.
MURATA Junichi, UENO Kazuyoshi, KOYANAGI Izumi, HOKIN Kiyohiro, TADA Mitsuhiro
Japanese Journal of National Medical Services, 40, 5, 400, 403, Japanese Society of National Medical Services, 1986
Japanese, 甲状腺癌はしばしば血行性転移を来すが, 頭蓋骨転移はまれである. 今回我々は甲状腺腫瘍の手術をしてから8年後に後頭部の皮下腫瘤に気づき, 翌年当科にて頭蓋骨腫瘍の診断で腫瘤を摘出し, 病理学的に甲状腺癌の転移と診断した1例を経験したので報告する.
患者は64才女性. 昭和50年に甲状腺片葉摘除術を受けている. 58年6月ころ, 後頭部の皮下腫瘤に気づき, 59年9月当科受診した. 神経学的陽性所見はなく, 頭部単純写にて後頭部正中に径8cm, 左前頭部に径1cmの骨欠損を認めた. 放射線学的に脳実質内に異常を認めなかつた. 外頸動脈のembolizationを行い, 翌日後頭部の腫瘍を全摘した. 病理診断は甲状腺濾胞癌の転移であつた.
文献上, 甲状腺癌の頭蓋骨転移で大きな腫瘤を形成した症例は少なく, これを全摘しえた例は本症例が初であると思われる.

Spinal epidural and subdural hemorrhage in hemophilia A. Case report　　　　　　　　　　　　　　　
K. Houkin, H. Abe, Y. Iwasaki
Nervous System in Children, 10, 1, 1, 5, 1985
Japanese, Scientific journal

iPS細胞を用いた神経疾患の病態解析と創薬 疾患特異的iPS細胞を用いたもやもや病の病態解析　　　　　　　　　　　　　　　
松尾 宗明, 灘中 里美, 曽我 美南, 江良 択実, 島野 健仁郎, 北川 裕之, 杉山 拓, 宝金 清博, 脳と発達, 56, Suppl., S121, S121, May 2024
(一社)日本小児神経学会, Japanese

もやもや病のエピゲノム病態修飾因子-血漿microRNA-探索
伊東雅基, 内野晴登, 東海林菊太郎, 杉山拓, 川堀真人, 岩田育子, 矢部一郎, 寳金清博, 藤村幹, 日本分子脳神経外科学会プログラム・抄録集, 24th, 2024

Comprehensive validation of circulating microRNA signatures for Moyamoya disease
伊東雅基, 内野晴登, 東海林菊太郎, 杉山拓, 川堀真人, 岩田育子, 矢部一郎, 寶金清博, 藤村幹, 月刊メディカル・サイエンス・ダイジェスト, 50, 6, 2024

成人もやもや病複合血行再建術後バイパス路発達におけるRNF213創始者変異の意義
伊東雅基, 川堀真人, 杉山拓, 東海林菊太郎, 舘澤諒大, 内野晴登, 数又研, 寳金清博, 藤村幹, Mt. Fuji Workshop on CVD, 39th, 2022

成人もやもや病に対する直接・間接複合血行再建術後のバイパス路発達の術前要因
伊東雅基, 内野晴登, 川堀真人, 杉山拓, 数又研, 宝金清博, 藤村幹, 日本分子脳神経外科学会プログラム・抄録集, 22nd, 2022

機械学習による歩行動画からのパーキンソン病患者UPDRSスコアの予測
江口 克紀, 白井 慎一, 松島 理明, 加納 崇裕, 山崎 和義, 濱内 祝嗣, 笹森 徹, 関 俊隆, 北川 まゆみ, 大槻 美佳, 寳金 清博, 佐々木 秀直, 矢部 一郎, パーキンソン病・運動障害疾患コングレスプログラム・抄録集, 15回, 75, 75, Jul. 2021
Movement Disorder Society of Japan (MDSJ), Japanese

日本学術会議in北海道 : 「ふるさと」から学術を考える (特集 地方学術会議)
寳金 清博, 学術の動向 = Trends in the sciences, 26, 6, 24, 27, Jun. 2021
日本学術協力財団, Japanese

治療法の再整理とアップデートのために専門家による私の治療 くも膜下出血
杉山 拓, 寳金 清博, 日本医事新報, 5067, 38, 39, Jun. 2021
(株)日本医事新報社, Japanese

パーキンソン病における両側STN-DBS後の体重増加と刺激位置との関連についての検討
江口 克紀, 矢部 一郎, 白井 慎一, 松島 理明, 加納 崇裕, 山崎 和義, 濱内 祝嗣, 笹森 徹, 関 俊隆, 北川 まゆみ, 大槻 美佳, 寳金 清博, 佐々木 秀直, パーキンソン病・運動障害疾患コングレスプログラム・抄録集, 14回, 83, 83, Feb. 2021
Movement Disorder Society of Japan (MDSJ), Japanese

RNF213創始者変異は成人もやもや病複合血行再建術後の間接バイパス路発達に寄与する
伊東雅基, 川堀真人, 杉山拓, 東海林菊太郎, 舘澤諒大, 内野晴登, 数又研, 寳金清博, 藤村幹, 脳循環代謝(Web), 33, 1, 2021

私の治療 くも膜下出血
杉山拓, 寳金清博, 週刊日本医事新報, 5067, 2021

Weight gain after subthalamic deep brain stimulation correlates with active contacts’position
江口克紀, 白井慎一, 山崎和義, 濱内祝嗣, 松島理明, 加納崇裕, 笹森徹, 平田健司, 関俊隆, 志賀哲, 大槻美佳, 寳金清博, 佐々木秀直, 矢部一郎, 日本定位・機能神経外科学会プログラム・抄録集(Web), 60回, 97, 97, Dec. 2020
(一社)日本定位・機能神経外科学会, Japanese

Cell Therapy against CNS Diseases : Current Status and Future Perspectives
川堀真人, 川堀真人, 七戸秀夫, 七戸秀夫, 黒田敏, 寳金清博, 脳神経外科ジャーナル, 29, 11, 777, 783, Nov. 2020
The Japanese Congress of Neurological Surgeons, Japanese

地域連携クリティカルパスが適用された急性心筋梗塞患者における予後規定要因の検討
小山 雅之, 樋室 伸顕, 佐藤 琢真, 簑島 暁帆, 竹内 利治, 橋本 暁佳, 齋藤 重幸, 三浦 哲嗣, 寳金 清博, 大西 浩文, 日本循環器病予防学会誌, 55, 3, 224, 224, Nov. 2020
(一社)日本循環器病予防学会, Japanese

DeFrictorによる経動脈的塞栓術についての検討　　　　　　　　　　　　　　　
長内 俊也, 東海林 菊太郎, 今井 哲秋, 杉山 拓, 中山 若樹, 数又 研, 寳金 清博, 脳血管内治療, 5, Suppl., 68, 68, Nov. 2020
(NPO)日本脳神経血管内治療学会, Japanese

パーキンソン病に対する深部脳刺激療法後の脳波β位相-広域γ振幅カップリングの変化に関する検討
江口 克紀, 矢部 一郎, 白井 慎一, 山崎 和義, 濱内 祝嗣, 松島 理明, 加納 崇裕, 笹森 徹, 関 俊隆, 大槻 美佳, 寳金 清博, 佐々木 秀直, 臨床神経生理学, 48, 5, 507, 507, Oct. 2020
(一社)日本臨床神経生理学会, Japanese

パーキンソン病に対する深部脳刺激療法後の脳波β位相-広域γ振幅カップリングの変化に関する検討
江口 克紀, 矢部 一郎, 白井 慎一, 山崎 和義, 濱内 祝嗣, 松島 理明, 加納 崇裕, 笹森 徹, 関 俊隆, 大槻 美佳, 寳金 清博, 佐々木 秀直, 臨床神経生理学, 48, 5, 534, 534, Oct. 2020
(一社)日本臨床神経生理学会, Japanese

Le FortI型骨切り術に合併したmaxillary動静脈瘻に対し血管内塞栓術を行った1例
後藤 秀輔, 長内 俊也, 牛越 聡, 栗栖 宏多, 下田 祐介, 寳金 清博, Neurological Surgery, 48, 4, 335, 340, Apr. 2020
(株)医学書院, Japanese

Cell therapy against cerebrovascular disease-Current status and future perspectives
川堀真人, 川堀真人, 七戸秀夫, 七戸秀夫, 黒田敏, 寳金清博, 臨床画像, 36, 4月増刊, 141, 150, Apr. 2020
(株)メジカルビュー社, Japanese

Le FortI型骨切り術に合併したmaxillary動静脈瘻に対し血管内塞栓術を行った1例
後藤 秀輔, 長内 俊也, 牛越 聡, 栗栖 宏多, 下田 祐介, 寳金 清博, Neurological Surgery, 48, 4, 335, 340, Apr. 2020
(株)医学書院, Japanese

化学療法導入直後に急激な嚢胞性増大を示した卵黄嚢腫瘍主体の頭蓋内胚細胞腫瘍の1例
澤谷 亮佑, 山口 秀, 伊師 雪友, 茂木 洋晃, 小林 浩之, 杉山 未奈子, 長 祐子, 井口 晶裕, 大塚 拓也, 今本 鉄平, 高桑 恵美, 寳金 清博, 脳神経外科速報, 30, 3, 300, 305, Mar. 2020
(株)メディカ出版, Japanese

Regenerative medicine using mesenchymal stem cell against central nervous system disease
川堀 真人, 寳金 清博, 医学のあゆみ, 272, 10, 1044, 1050, Mar. 2020
医歯薬出版(株), Japanese

化学療法導入直後に急激な嚢胞性増大を示した卵黄嚢腫瘍主体の頭蓋内胚細胞腫瘍の1例　　　　　　　　　　　　　　　
澤谷 亮佑, 山口 秀, 伊師 雪友, 茂木 洋晃, 小林 浩之, 杉山 未奈子, 長 祐子, 井口 晶裕, 大塚 拓也, 今本 鉄平, 高桑 恵美, 寳金 清博, 脳神経外科速報, 30, 3, 300, 305, Mar. 2020
(株)メディカ出版, Japanese

大学病院の医療事故調査制度への対応 本制度の10の課題
中村 伸理子, 有賀 徹, 寳金 清博, 嘉山 孝正, 橋本 重厚, 堤 晴彦, 中島 勧, 岩瀬 博太郎, 米村 滋人, 櫻井 淳, 三木 保, 吉田 謙一, 大嶽 浩司, 小林 弘幸, 坂本 哲也, 大磯 義一郎, 中島 和江, 水谷 渉, 全国医学部長病院長会議大学病院の医療事故対策委員会, 日本医師会雑誌, 148, 11, 2213, 2218, Feb. 2020
(公社)日本医師会, Japanese

経鼻内視鏡手術を下支えする鞍底再建技術の変遷とその成果
小林浩之, 寺坂俊介, 茂木洋晃, 山口秀, 伊師雪友, 浅岡克行, 宝金清博, 宝金清博, 日本間脳下垂体腫瘍学会プログラム・抄録集, 30th, 2020

Cell therapy against central nervous disorders – Current and future perspectives –
Kawabori Masahito, Shichinohe Hideo, Kuroda Satoshi, Houkin Kiyohiro, Neurological Therapeutics, 37, 3, 386, 390, 2020

Stem cell therapy for central nervous system disorders have launched from basic research to the clinical trial phase. Many clinical trials are currently executed in Japan including ischemic stroke, traumatic brain injury, Parkingson's disease, and spinal cord injury. The preliminary data from these trials are promising and there is a strong expectation both from the medical and patient point of view. However, there are still so many unsolved questions which require further experimental procedures. The questions include cell types, transplantation route, transplantation timing and cell dosages. This article will highlight the basic knowledge of stem cell and the preliminary result of clinical trials launched in Japan.

, Japanese Society of Neurological Therapeutics, Japanese

パーキンソン病に対する深部脳刺激療法後の脳波β位相-広域γ振幅カップリングの変化に関する検討
江口克紀, 矢部一郎, 白井慎一, 山崎和義, 濱内祝嗣, 松島理明, 加納崇裕, 笹森徹, 関俊隆, 大槻美佳, 寳金清博, 佐々木秀直, 臨床神経生理学(Web), 48, 5, 2020

パーキンソン病に対する深部脳刺激療法後の脳波β位相-広域γ振幅カップリングの変化に関する検討
江口克紀, 矢部一郎, 白井慎一, 山崎和義, 濱内祝嗣, 松島理明, 加納崇裕, 笹森徹, 関俊隆, 大槻美佳, 寳金清博, 佐々木秀直, 臨床神経生理学(Web), 48, 5, 2020

DeFrictorによる経動脈的塞栓術についての検討
長内俊也, 東海林菊太郎, 今井哲秋, 杉山拓, 中山若樹, 数又研, 寳金清博, 脳血管内治療(Web), 5, Supplement, 2020

The influence of surgical intervention for high-dose Methotrexate chemotherapy in the patients with primary central nervous system lymphoma
岡本迪成, 山口秀, 伊師雪友, 茂木洋晃, 小林浩之, 寺坂俊介, 寳金清博, 日本脳腫瘍学会プログラム・抄録集, 38th, 2020