Dec. 2024, 浜松電子工学奨励会, 高柳研究奨励賞　　　　　　　　　　　　　　　
超高速かつ超広視野な回折限界集光技術の開発
渋川 敦史

Feb. 2021, 公益財団法人コニカミノルタ科学技術振興財団, 令和２年度コニカミノルタ画像科学奨励賞　　　　　　　　　　　　　　　
世界最速一次元空間光変調器の開発と光波面シェイピングへの応用
渋川 敦史

Large-volume focus control at 10 MHz refresh rate via fast line-scanning amplitude-encoded scattering-assisted holography
Atsushi Shibukawa, Ryota Higuchi, Gookho Song, Hideharu Mikami, Yuki Sudo, Mooseok Jang
Nature Communications, 15, 1, Springer Science and Business Media LLC, 08 Apr. 2024, [Peer-reviewed], [Lead author]
Scientific journal, Abstract

The capability of focus control has been central to optical technologies that require both high temporal and spatial resolutions. However, existing varifocal lens schemes are commonly limited to the response time on the microsecond timescale and share the fundamental trade-off between the response time and the tuning power. Here, we propose an ultrafast holographic focusing method enabled by translating the speed of a fast 1D beam scanner into the speed of the complex wavefront modulation of a relatively slow 2D spatial light modulator. Using a pair of a digital micromirror device and a resonant scanner, we demonstrate an unprecedented refresh rate of focus control of 31 MHz, which is more than 1,000 times faster than the switching rate of a digital micromirror device. We also show that multiple micrometer-sized focal spots can be independently addressed in a range of over 1 MHz within a large volume of 5 mm × 5 mm × 5.5 mm, validating the superior spatiotemporal characteristics of the proposed technique – high temporal and spatial precision, high tuning power, and random accessibility in a three-dimensional space. The demonstrated scheme offers a new route towards three-dimensional light manipulation in the 100 MHz regime.

Detection of Membrane Potential-Dependent Rhodopsin Fluorescence Using Low-Intensity Light Emitting Diode for Long-Term Imaging
Shiho Kawanishi, Keiichi Kojima, Atsushi Shibukawa, Masayuki Sakamoto, Yuki Sudo
ACS Omega, 8, 4826, 4834, American Chemical Society (ACS), 25 Jan. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal

Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization.
Masuzu Kikuchi, Keiichi Kojima, Shin Nakao, Susumu Yoshizawa, Shiho Kawanishi, Atsushi Shibukawa, Takashi Kikukawa, Yuki Sudo
Scientific reports, 11, 1, 14765, 14765, 20 Jul. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Microbial rhodopsins are photoswitchable seven-transmembrane proteins that are widely distributed in three domains of life, archaea, bacteria and eukarya. Rhodopsins allow the transport of protons outwardly across the membrane and are indispensable for light-energy conversion in microorganisms. Archaeal and bacterial proton pump rhodopsins have been characterized using an Escherichia coli expression system because that enables the rapid production of large amounts of recombinant proteins, whereas no success has been reported for eukaryotic rhodopsins. Here, we report a phylogenetically distinct eukaryotic rhodopsin from the dinoflagellate Oxyrrhis marina (O. marina rhodopsin-2, OmR2) that can be expressed in E. coli cells. E. coli cells harboring the OmR2 gene showed an outward proton-pumping activity, indicating its functional expression. Spectroscopic characterization of the purified OmR2 protein revealed several features as follows: (1) an absorption maximum at 533 nm with all-trans retinal chromophore, (2) the possession of the deprotonated counterion (pKa = 3.0) of the protonated Schiff base and (3) a rapid photocycle through several distinct photointermediates. Those features are similar to those of known eukaryotic proton pump rhodopsins. Our successful characterization of OmR2 expressed in E. coli cells could build a basis for understanding and utilizing eukaryotic rhodopsins.

Green-Sensitive, Long-Lived, Step-Functional Anion Channelrhodopsin-2 Variant as a High-Potential Neural Silencing Tool
Keiichi Kojima, Natsuki Miyoshi, Atsushi Shibukawa, Srikanta Chowdhury, Masaki Tsujimura, Tomoyasu Noji, Hiroshi Ishikita, Akihiro Yamanaka, Yuki Sudo
The Journal of Physical Chemistry Letters, 11, 15, 6214, 6218, American Chemical Society (ACS), 22 Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Anion channelrhodopsin-2 (GtACR2) was identified from the alga Guillardia theta as a light-gated anion channel, providing a powerful neural silencing tool for optogenetics. To expand its molecular properties, we produced here GtACR2 variants by strategic mutations on the four residues around the retinal chromophore (i.e., R129, G152, P204, and C233). After the screening with the Escherichia coli expression system, we estimated spectral sensitivities and the anion channeling function by using the HEK293 expression system. Among the mutants, triple (R129M/G152S/C233A) and quadruple (R129M/G152S/P204T/C233A) mutants showed the significantly red-shifted absorption maxima (λmax = 498 and 514 nm, respectively) and the long-lived channel-conducting states (the half-life times were 3.4 and 5.4 s, respectively). In addition, both mutants can be activated and inactivated by different wavelengths, representing their step-functional ability. We nicknamed the quadruple mutant "GLaS-ACR2" from its green-sensitive, long-lived, step-functional properties. The unique characteristics of GLaS-ACR2 suggest its high potential as a neural silencing tool.

The Unlimited Potential of Microbial Rhodopsins as Optical Tools　　　　　　　　　　　　　　　
K. Kojima, A. Shibukawa, Y. Sudo
Biochemistry, 59, 3, 218, 229, Dec. 2019, [Peer-reviewed], [Invited]
English, Scientific journal

Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination　　　　　　　　　　　　　　　
T. Yamanashi, M. Maki, K. Kojima, A. Shibukawa, T. Tsukamoto, S. Chowdhury, A. Yamanaka, S. Takagi, Y. Sudo
Scientific Reports, 9, 7863, May 2019, [Peer-reviewed]
English, Scientific journal

Photochemical Characterization of a New Heliorhodopsin from the Gram-Negative Eubacterium Bellilinea caldifistulae (BcHeR) and Comparison with Heliorhodopsin-48C12　　　　　　　　　　　　　　　
A. Shibukawa, K. Kojima, Y. Nakajima, Y. Nishimura, S. Yoshizawa, Y. Sudo
Biochemistry, 58, 26, 2934, 2943, May 2019, [Peer-reviewed], [Lead author]
English, Scientific journal

Virtual phase conjugation based optical tomography for single-shot three-dimensional imaging　　　　　　　　　　　　　　　
Yuta Goto, Atsushi Okamoto, Atsushi Shibukawa, Kazuhisa Ogawa, Akihisa Tomita
Optics Express, 26, 3779, 3790, Feb. 2018, [Peer-reviewed]
English, Scientific journal

Wavefront engineering with disorder-engineered metasurfaces
Mooseok Jang†, Yu Horie†, Atsushi Shibukawa†, Joshua Brake, Yan Liu, Seyedeh Mahsa Kamali, Amir Arbabi, HaowenRuan, Andrei Faraon, Changhuei Yang
Nature Photonics, 12, 84, 90, Feb. 2018, [Peer-reviewed], [Lead author]
English, Scientific journal

Glare suppression by coherence gated negation
Edward Haojiang Zhou, Atsushi Shibukawa, Joshua Brake, Haowen Ruan, Changhuei Yang
OPTICA, 3, 10, 1107, 1113, Oct. 2016, [Peer-reviewed]
English, Scientific journal

Towards new applications using capillary waveguides
Nicolino Stasio†, Atsushi Shibukawa†, Ioannis N, Papadopoulos, Salma Farahi, Olivier Simandoux, Jean-Pierre Huignard, Emmanuel Bossy, Christophe Moser, Demetri Psaltis
BIOMEDICAL OPTICS EXPRESS, 6, 12, 4619, 4631, Dec. 2015, [Peer-reviewed], [Lead author]
English, Scientific journal

Two-channel algorithm for single-shot, high-resolution measurement of optical wavefronts using two image sensors
Jin Nozawa, Atsushi Okamoto, Atsushi Shibukawa, Masanori Takabayashi, Akihisa Tomita
APPLIED OPTICS, 54, 29, 8644, 8652, Oct. 2015, [Peer-reviewed]
English, Scientific journal

Background light reduction method with a double phase conjugate mirror and a phase plate for optical inter-satellite communications
Kaori Nishimaki, Atsushi Okamoto, Atsushi Shibukawa, Akihisa Tomita, Yoshihisa Takayama, Masatoshi Bunsen
JAPANESE JOURNAL OF APPLIED PHYSICS, 53, 8, 08MB13, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Dynamically Reconfigurable Characteristics of a Double Phase Conjugate Mirror Using Sn2P2S6 Crystals and Their Application to Optical Inter-Satellite Communication
Kaori Nishimaki, Atsushi Okamoto, Atsushi Shibukawa, Masanori Takabayashi, Akihisa Tomita, Yoshihisa Takayama
OPTICAL REVIEW, 21, 3, 415, 424, May 2014, [Peer-reviewed]
English, Scientific journal

Digital phase conjugate mirror by parallel arrangement of two phase-only spatial light modulators
Atsushi Shibukawa, Atsushi Okamoto, Yuta Goto, Satoshi Honma, Akihisa Tomita
OPTICS EXPRESS, 22, 10, 11918, 11929, May 2014, [Peer-reviewed], [Lead author]
English, Scientific journal

Study on ultra-high density holographic memory using optical complex-amplitude control technique　　　　　　　　　　　　　　　
Atsushi Shibukawa
Hokkaido University, Mar. 2014, [Peer-reviewed], [Invited]
Japanese, Doctoral thesis

Spatial cross modulation method using a random diffuser and phase-only spatial light modulator for constructing arbitrary complex fields
Atsushi Shibukawa, Atsushi Okamoto, Masanori Takabayashi, Akihisa Tomita
OPTICS EXPRESS, 22, 4, 3968, 3982, Feb. 2014, [Peer-reviewed], [Lead author]
English, Scientific journal

Reconfigurable spatial mode conversion using a phase-type spatial light modulator　　　　　　　　　　　　　　　
Tomohiro Maeda, Atsushi Okamoto, Yuki Hirasaki, Atsushi Shibukawa, Akihisa Tomita
2014 OptoElectronics and Communication Conference, OECC 2014 and Australian Conference on Optical Fibre Technology, ACOFT 2014, 517, 518, IEEE Computer Society, 2014
English, International conference proceedings

Double-Referential Holography and Spatial Quadrature Amplitude Modulation
Keisuke Zukeran, Atsushi Okamoto, Masanori Takabayashi, Atsushi Shibukawa, Kunihiro Sato, Akihisa Tomita
JAPANESE JOURNAL OF APPLIED PHYSICS, 52, 9, 09LD13, Sep. 2013, [Peer-reviewed]
English, Scientific journal

Digital Image Diffusion Technique for Suppressing Interpage Crosstalk in Holographic Data Storage
Atsushi Shibukawa, Atsushi Okamoto, Yuta Wakayama, Akihisa Tomita, Hisatoshi Funakoshi, Kunihiro Sato
JAPANESE JOURNAL OF APPLIED PHYSICS, 52, 9, 09LD03, Sep. 2013, [Peer-reviewed], [Lead author]
English, Scientific journal

Multilayer Collinear Holographic Memory for Ultra-High Density Recording　　　　　　　　　　　　　　　
Atsushi Okamoto, Atsushi Shibukawa
光学, 40, 11, 591, 593, Nov. 2011, [Invited]
Japanese, Scientific journal

Multilayer Collinear Holographic Memory with Movable Random Phase Mask
Atsushi Shibukawa, Atsushi Okamoto, Akihisa Tomita, Masanori Takabayashi, Kunihiro Sato
JAPANESE JOURNAL OF APPLIED PHYSICS, 50, 9, 09ME10, Sep. 2011, [Peer-reviewed]
English, Scientific journal

Evaluation of the performance in multilayer collinear holographic memory with movable random phase mask
Atsushi Shibukawa, Atsushi Okamoto, Akihisa Tomita, Masanori Takabayashi, Kunihiro Sato, Masatoshi Bunsen
Optics InfoBase Conference Papers, Optical Society of America (OSA), 2011
English, International conference proceedings

High-speed full motion analysis based on FFT-BPM for collinear holographic memory
Hisatoshi Funakoshi, Atsushi Okamoto, Masanori Takabayashi, Yuta Wakayama, Atsushi Shibukawa
Optics InfoBase Conference Papers, Optical Society of America (OSA), 2011
English, International conference proceedings

Double-referential collinear holographic memory and spatial quadrature amplitude modulation　　　　　　　　　　　　　　　
Atsushi Okamoto, Keisuke Zukeran, Atsushi Shibukawa, Masanori Takabayashi
Optics InfoBase Conference Papers, Optical Society of American (OSA), 2011
English, International conference proceedings

Basic Operation Simulation of Novel 3D Display Based on High-speed Analysis Method for Volume Hologram
舟越 久敏, 岡本 淳, 渋川 敦史, 電子情報通信学会技術研究報告 = IEICE technical report : 信学技報, 115, 459, 309, 313, 22 Feb. 2016
電子情報通信学会, Japanese

Highly accurate spatial mode generation using spatial cross modulation method for mode division multiplexing
Hiroki Sakuma, Atsushi Okamoto, Atsushi Shibukawa, Yuta Goto, Akihisa Tomita, Next-Generation Optical Communication: Components, Sub-Systems, and Systems V, 13 Feb. 2016
SPIE

High-resolution and simultaneous measurement along the depth direction using virtual phase conjugation for optical tomography
Yuta Goto, Atsushi Okamoto, Atsushi Shibukawa, Akihisa Tomita, Masanori Takabayashi, 2015 20th Microoptics Conference (MOC), Oct. 2015
IEEE

Optical tomography using a random diffuser and digital phase conjugation
Yuta Goto, Atsushi Okamoto, Atsushi Shibukawa, Akihisa Tomita, Kunihiro Sato, Optical Tomography and Spectroscopy of Tissue XI, 05 Mar. 2015
SPIE

Numerical analysis of optical tomographic imaging using virtual phase conjugation
後藤 優太, 岡本 淳, 渋川 敦史, 電子情報通信学会技術研究報告 = IEICE technical report : 信学技報, 114, 460, 9, 14, 23 Feb. 2015
電子情報通信学会, Japanese

Numerical analysis of optical tomographic imaging using virtual phase conjugation
後藤 優太, 岡本 淳, 渋川 敦史, 電子情報通信学会技術研究報告 = IEICE technical report : 信学技報, 114, 459, 9, 14, 23 Feb. 2015
電子情報通信学会, Japanese

3次元表示に向けた空間クロスモジュレーション法
菅悠太, 岡本淳, 渋川敦史, 富田章久, 舟越久敏, 応用物理学会秋季学術講演会講演予稿集(CD-ROM), 75th, 2014

Recording and Reconstruction of 38-Level Spatial Quadrature-Amplitude Modulation Signal in Double-Referential Holographic Memory
ZUKERAN Keisuke, OKAMOTO Atsushi, TAKABAYASHI Masanori, SHIBUKAWA Atsushi, TOMITA Akihisa, ITE Technical Report, 37, 10, 1, 7, 15 Feb. 2013
We propose a double-referential holographic memory (DRHM) that can detect phase modulation signal by just entering reference beam into recording medium without additional system for generation and irradiation of the beam to interfere with signal beam. In this technique, by highly-accurate and simplified complex amplitude detection, high multi-level phase modulation signal and spatial quadrature-amplitude modulation (SQAM) signal become available, so it is expected that storage capacity and data transfer rate are greatly increased. In this paper, we describe the basic operation of the DRHM and evaluate multiple recording/reconstruction characteristics by numerical simulation. Besides, the method for furthermore improving reconstruction quality is newly proposed and its effectiveness is demonstrated., The Institute of Image Information and Television Engineers, Japanese

Wavefront shaping-assisted subcellular-resolution focus scanning over centimeter-scale field-of-view　　　　　　　　　　　　　　　
Atsushi Shibukawa
The 13th Asia-Pacific Laser Symposium 2025, 16 May 2025, English, Invited oral presentation
12 May 2025 - 17 May 2025, [Invited]

Wavefront shaping-assisted multi-regional two-photon microscopy for recording distributed neuronal activity across the mouse cortex　　　　　　　　　　　　　　　
Hyojeong Shon, Atsushi Shibukawa, Hideharu Mikami
Focus on microscopy 2025, 15 Apr. 2025, English, Poster presentation

Accelerating complex wavefront shaping with line-scanning holography using digital micromirror devices　　　　　　　　　　　　　　　
Mooseok Jang, Atsushi Shibukawa
SPIE Photonics West, English, Invited oral presentation
25 Jan. 2025 - 30 Jan. 2025, [Invited]

Ultra-wide field-of-view optical focus control with high-speed complex wavefront shaping　　　　　　　　　　　　　　　
Atsushi Shibukawa
The 85th JSAP Autumn Meeting 2024, Sep. 2024, English, Invited oral presentation
16 Sep. 2024 - 20 Sep. 2024, [Invited]

Wavefront shaping at 10 MHz refresh rate by exploiting the scan speed of a resonant scanner　　　　　　　　　　　　　　　
Atsushi Shibukawa, Yuki Sudo, Hideharu Mikami, Mooseok Jang
The Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR) 2024, Aug. 2024, English, Invited oral presentation
[Invited]

Random-access focus scanning at 10MHz refresh rate by a scattering-assisted lens based on an ultrafast 1D spatial light modulation　　　　　　　　　　　　　　　
Atsushi Shibukawa, Yuki Sudo, Hideharu Mikami, Mooseok Jang
Optics & Photonics Japan, 28 Nov. 2023, Japanese, Nominated symposium
27 Nov. 2023 - 29 Nov. 2023, [Invited]

Development of ultra-large field-of-view focusing lens by complex wavefront compensation towards mouse brain cortex-wide imaging　　　　　　　　　　　　　　　
Atsushi Shibukawa, Hyojeong Shon, Hideharu Mikami
Optics & Photonics Japan, 27 Nov. 2023, Japanese, Poster presentation
27 Nov. 2023 - 29 Nov. 2023

LED光源を用いたアーキロドプシン3（AR3）による長時間膜電位計測法の確立　　　　　　　　　　　　　　　
川西 志歩, 小島 慧一, 渋川 敦史, 坂本 雅行, 須藤 雄気
第43回生体膜と薬物の相互作用シンポジウム, 07 Oct. 2022, English

LED光源を用いた微生物型ロドプシンAR3による膜電位の長時間イメージング　　　　　　　　　　　　　　　
川西 志歩, 小島 慧一, 渋川 敦史, 坂本 雅行, 須藤 雄気
第60回日本生物物理学会年会, 29 Sep. 2022, English, Poster presentation

Megahertz Wavefront Control through Scattering Media　　　　　　　　　　　　　　　
Atsushi Shibukawa, Hideharu Mikami, Mooseok Jang, Yuki Sudo
The 22nd RIES-Hokudai International Symposium, 06 Dec. 2021, English, Poster presentation

コンプレックス波面整形による光散乱との共生　　　　　　　　　　　　　　　
渋川敦史
コニカミノルタカレッジ「先端光学」, 02 Dec. 2021, Japanese, Public discourse
[Invited]

Ultrafast wavefront control involving scattering media　　　　　　　　　　　　　　　
Atsushi Shibukawa, Jang Mooseok, Hideharu Mikami, Yuki Sudo
International Symposium on Imaging, Sensing, and Optical Memory 2021, 04 Oct. 2021, English, Invited oral presentation
[Invited]

LED光源を用いたアーキロドプシン3由来の膜電位感受性蛍光の観察　　　　　　　　　　　　　　　
川西志歩, 小島慧一, 渋川敦史, 坂本雅行, 須藤雄気
第12回日本生物物理学会 中国四国支部大会, 22 May 2021, Japanese, Poster presentation