Oct. 2018, 一般財団法人バイオインダストリー協会, バイオインダストリー奨励賞　　　　　　　　　　　　　　　
配列制御型ポリエステル生合成系の開発
松本謙一郎

2016, the Minister of Education, Culture, Sports, Science and Technology, Prizes for Science and Technology　　　　　　　　　　　　　　　
The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology
Ken'ichiro Matsumoto

2008, The Society of Polymer Science, Japan, Award for Encouragement of Research in Polymer Science　　　　　　　　　　　　　　　
Ken'ichiro Matsumoto

18-Crown-6 ether increases molecular weight of poly(3-hydroxybutyrate) synthesized in Escherichia coli　　　　　　　　　　　　　　　
Seiya Tanaka, Hiroshi Kikukawa, HirotsuguNakai, Shin-ichiHachisuka, Ken'ichiro Matsumoto
Journal of Bioscience and Bioengineering, in press, Jan. 2026, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Single-cell ATP monitoring in Escherichia coli engineered for polyhydroxybutyrate production using the fluorescent biosensor QUEEN
Wataru Fuji, Ayaka Kajikawa, Shin-ichi Hachisuka, Hiroya Tomita, Hiroshi Kikukawa, Hiromi Imamura, Ken'ichiro Matsumoto
Journal of Bioscience and Bioengineering, Jan. 2026, [Peer-reviewed], [Last author]
English, Scientific journal

Biosynthesis of poly(6-hydroxyhexanoate) [poly(ε-caprolactone)] using engineered polyhydroxyalkanoate synthetic system in Escherichia coli
Kengo Yanagawa, Shin-ichi Hachisuka, Haruno Kusumoto, Kazuki Yamamoto, Shoko Furukawa, Mamoru Sasaki, Kyogo Iseki, Naoya Nakagawa, Shogo Nakano, Hiroshi Kikukawa, Ken’ichiro Matsumoto
Journal of Bioscience and Bioengineering, Dec. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Aprotic polyether-induced decrease in the molecular weight of polyhydroxyalkanoate provides insights into the mechanism of action of chain-transfer agents
Seiya Tanaka, Hiroshi Kikukawa, Hirotsugu Nakai, Shuzo Arai, Ayaka Kajikawa, Shin-ichi Hachisuka, Ken’ichiro Matsumoto
Polymer Degradation and Stability, 242, 111656, Dec. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Microbial degradation of the artificial polyhydroxyalkanoate poly(2-hydroxybutyrate-co-3-hydroxybutyrate) with random and block sequences using soil bacteria
Shin-ichi Hachisuka, Ami Iguchi, Hiroshi Kikukawa, Hiroya Tomita, Toshihiko Ooi, Ken'ichiro Matsumoto
Journal of Applied Microbiology, 136, 6, lxaf123, 02 Jun. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Identification of an Extracellular Poly(3-hydroxybutyrate) Depolymerase in the Genus Alteromonas and Its Phylogenetic Distribution Among Alteromonas Species
Kyogo Iseki, Shin-ichi Hachisuka, Hiroshi Kikukawa, Takeharu Tsuge, Ken’ichiro Matsumoto
Marine Biotechnology, 27, 95, Jun. 2025, [Peer-reviewed], [Last author]
English, Scientific journal

Tacticity difference in the incorporation of 4-hydroxyalkanoate units with 2-, 3-, or 4-methyl side chains into polyhydroxyalkanoate (PHA) using the engineered PHA synthase PhaCAR
Ken’ichiro Matsumoto, Nagi Isobe, Hiroya Tomita
Polymer Degradation and Stability, 239, 111422, May 2025, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Insights into widespread disturbance in gene expression and severe growth inhibition observed in transgenic rice producing polyhydroxybutyrate
Hiroaki Shimada, Astuo Kawamura, Miki Ogasawara, Aya Tamaki, Tetsuya Yamazaki, Yohei Igarashi, Sota Hara, Chiaki Yamagiwa, Hiroshi Teramura, Hiroaki Kusano, Ken’ichiro Matsumoto
Plant Biotechnology, 42, 1, 41, 49, Japanese Society for Plant Cell and Molecular Biology, 25 Mar. 2025, [Peer-reviewed]
English, Scientific journal

Creation of sequence-regulating polyhydroxyalkanoate (PHA) synthases with improved thermostability using a full consensus design for the biosynthesis of 2-hydroxyalkanoate-based PHA block copolymers
Shoko Furukawa, Naoya Nakagawa, Shin-ichi Hachisuka, Shogo Nakano, Hiroya Tomita, Hiroshi Kikukawa, Ken'ichiro Matsumoto
Polymer Degradation and Stability, 111295, 111295, Elsevier BV, Feb. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Utilization of duckweed-derived biomass for polyhydroxybutyrate production in Escherichia coli via dual enzymatic saccharification using amylase and cellulase complex
Toshihiko Ooi, Rikako Sato, Ken'ichiro Matsumoto
Bioscience, Biotechnology, and Biochemistry, 88, 12, 1496, 1499, Oxford University Press (OUP), 12 Sep. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, Abstract

Duckweed is a rapid-growing plant with a high starch and low lignin content. The duckweed was saccharified via dual enzymatic treatment using amylase and cellulase complex. The duckweed-derived glucose was utilized for polyhydroxybutyrate (PHB) production in engineered Escherichia coli. In addition, the low concentration supplementation of the duckweed extract promoted cell growth compared to the analytical grade glucose.

Biosynthesis of High Toughness Poly(3‐Hydroxypropionate)‐Based Block Copolymers With Poly(D‐2‐Hydroxybutyrate) and Poly(D‐Lactate) Segments Using Evolved Monomer Sequence‐Regulating Polyester Synthase
Tomoya Kawakami, Hiroya Tomita, Phan Thi Hien, Ken'ichiro Matsumoto
Biopolymers, 115, 6, Wiley, 08 Aug. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, ABSTRACT

This study synthesized poly(3‐hydroxypropionate) [P(3HP)]‐containing polyhydroxyalkanoate (PHA) block copolymers, P(3HP)‐b‐P[2‐hydroxybutyrate (2HB)] and P(3HP)‐b‐P(D‐lactate) (PDLA), using Escherichia coli. The cells expressing an evolved sequence‐regulating PHA synthase, PhaC_ARNDFH, and propionyl‐CoA transferase were cultured with the supplementation of the corresponding monomer precursors in the medium. The block structure of P(3HP)‐b‐PDLA was confirmed by proton nuclear magnetic resonance analysis and solvent fractionation. The molecular weights of the polymers were in the range of 0.8–2.8 × 10⁵. The solvent‐cast polymer films were subjected to isothermal treatment to promote phase separation and crystallization and were subsequently melt‐quenched to produce an amorphous phase. The melt‐quenched P(3HP)‐b‐P(2HB) film exhibited a high elongation at break (1153%), resulting in a toughness of 181 MJ/m³. The solvent‐cast film of P(3HP)‐b‐65 mol% PDLA exhibited partial elastic deformation, in which the P(3HP) phase functioned as a soft segment. The melt‐quenching of the polymer resulted in embrittlement presumably due to the high lactate fraction. Overall, the P(3HP)‐based block copolymers exhibited several mechanical properties depending on the higher‐order structure of the polymer and the properties of the P(2‐hydroxyalkanoate) segments. This study findings show that P(3HP)‐b‐P(2HB) and P(3HP)‐b‐PDLA can function excellently if their microstructures are properly controlled.

Engineering of the Long-Main-Chain Monomer-Incorporating Polyhydroxyalkanoate Synthase PhaCAR for the Biosynthesis of Poly[ (R)-3-hydroxybutyrate-co-6-hydroxyhexanoate]
Yuka Hozumi, Shin-ichi Hachisuka, Hiroya Tomita, Hiroshi Kikukawa, Ken’ichiro Matsumoto
Biomacromolecules, 25, 2973, 2979, 13 May 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Toward the production of block copolymers in microbial cells: achievements and perspectives
Ken’ichiro Matsumoto
Applied Microbiology and Biotechnology, 108, 164, Jan. 2024, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
Scientific journal

(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha
Shizuru Ishihara, Izumi Orita, Ken’ichiro Matsumoto, Toshiaki Fukui
Applied Microbiology and Biotechnology, Springer Science and Business Media LLC, 29 Sep. 2023, [Peer-reviewed]
English, Scientific journal, Abstract

Bacterial polyhydroxyalkanoates (PHAs) are promising bio-based biodegradable polyesters. It was recently reported that novel PHA block copolymers composed of (R)-3-hydroxybutyrate (3HB) and (R)-2-hydroxybutyrate (2HB) were synthesized by Escherichia coli expressing PhaC_AR, a chimeric enzyme of PHA synthases derived from Aeromonas caviae and Ralstonia eutropha. In this study, the sequence-regulating PhaC_AR was applied in the natural PHA-producing bacterium, R. eutropha. During the investigation, (R/S)-2HB was found to exhibit strong growth inhibitory effects on the cells of R. eutropha. This was probably due to formation of excess 2-ketobutyrate (2KB) from (R/S)-2HB and the consequent l-valine depletion caused by dominant l-isoleucine synthesis attributed to the excess 2KB. Deletion analyses for genes of lactate dehydrogenase homologs identified cytochrome-dependent d-lactate dehydrogenase (Dld) and [Fe-S] protein-dependent l-lactate dehydrogenase as the enzymes responsible for sensitivity to (R)-2HB and (S)-2HB, respectively. The engineered R. eutropha strain (phaC_AR⁺, ldhA_Cd-hadA_Cd⁺ encoding clostridial (R)-2-hydroxyisocaproate dehydrogenase and (R)-2-hydoroxyisocaproate CoA transferase, ∆dld) synthesized PHA containing 10 mol% of 2HB when cultivated on glucose with addition of sodium (RS)-2HB, and the 2HB composition in PHA increased up to 35 mol% by overexpression phaC_AR. The solvent fractionation and NMR analyses showed that the resulting PHAs were most likely to be block polymers consisting of P(3HB-co-3HV) and P(2HB) segments, suggesting that PhaC_AR functions as the sequence-regulating PHA synthase independently from genetic and metabolic backgrounds of the host cell.

Key points

(R/S)-2-hydroxubutyrates (2HB) caused l-valine deletion in Ralstonia eutropha(R)- and (S)-lactate/2HB dehydrogenases functional in R. eutropha were identifiedThe engineered R. eutropha synthesized block copolymers of 2HB-containing polyhydroxyalkanoates on glucose and 2HB Graphical Abstract

Biosynthesis of High-Molecular-Weight Poly(d-lactate)-Containing Block Copolyesters Using Evolved Sequence-Regulating Polyhydroxyalkanoate Synthase PhaC_AR
Hien Thi Phan, Shoko Furukawa, Koto Imai, Hiroya Tomita, Takuya Isono, Toshifumi Satoh, Ken’ichiro Matsumoto
ACS Sustainable Chemistry and Engineering, 11, 30, 11123, 11129, American Chemical Society (ACS), 17 Jul. 2023, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Real-time NMR analysis of polyhydroxyalkanoate synthase reaction that synthesizes block copolymer comprising glycolate and 3-hydroxybutyrate
Kengo Yanagawa, Ayaka Kajikawa, Sayaka Sakakibara, Hiroyuki Kumeta, Hiroya Tomita, Ken'ichiro Matsumoto
Biophysical Chemistry, 296, 107001, 107001, Elsevier BV, May 2023, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Heat-Press Technique for Coating Ammonium Nitrate Granules with Biodegradable Hydrophobic Polymer Blend of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) and Poly(ε-caprolactone)
Chinonso Anthony Ezema, Toshihiko Ooi, Ken’ichiro Matsumoto
ACS Agricultural Science and Technology, 2, 6, 1179, 1186, American Chemical Society (ACS), 23 Nov. 2022, [Peer-reviewed]
Scientific journal

Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR
Keigo Satoh, Tomoya Kawakami, Nagi Isobe, Loïc Pasquier, Hiroya Tomita, Manfred Zinn, Ken’ichiro Matsumoto
Microbial Cell Factories, 21, 1, 84, Springer Science and Business Media LLC, May 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, Abstract

Background

Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence. The development of PhaC_AR, a unique sequence-regulating PHA synthase, has enabled the spontaneous biosynthesis of PHA block copolymers. PhaC_AR synthesizes both a block copolymer poly(2-hydroxybutyrate)-b-poly(3-hydroxybutyrate) [P(2HB)-b-P(3HB)], and a random copolymer, poly(3HB-co-3-hydroxyhexanoate), indicating that the combination of monomers determines the monomer sequence. Therefore, in this study, we explored the substrate scope of PhaC_AR and the monomer sequences of the resulting copolymers to identify the determinants of the monomer sequence. PhaC_AR is a class I PHA synthase that is thought to incorporate long-main-chain hydroxyalkanoates (LMC HAs, > C₃ in the main [backbone] chain). Thus, the LMC monomers, 4-hydroxy-2-methylbutyrate (4H2MB), 5-hydroxyvalerate (5HV), and 6-hydroxyhexanoate (6HHx), as well as 2HB, 3HB, and 3-hydroxypropionate (3HP) were tested.

Results

Recombinant Escherichia coli harboring PhaC_AR, CoA transferase and CoA ligase genes was used for PHA production. The medium contained the monomer precursors, 2HB, 3HB, 3HP, 4H2MB, 5HV, and 6HHx, either individually or in combination. As a result, homopolymers were obtained only for 3HB and 3HP. Moreover, 3HB and 3HP were randomly copolymerized by PhaC_AR. 3HB-based binary copolymers P(3HB-co-LMC HA)s containing up to 2.9 mol% 4H2MB, 4.8 mol% 5HV, or 1.8 mol% 6HHx were produced. Differential scanning calorimetry analysis of the copolymers indicated that P(3HB-co-LMC HA)s had a random sequence. In contrast, combining 3HP and 2HB induced the synthesis of P(3HP)-b-P(2HB). Similarly, P(2HB) segment-containing block copolymers P(3HB-co-LMC HA)-b-P(2HB)s were synthesized. Binary copolymers of LMC HAs and 2HB were not obtained, indicating that the 3HB or 3HP unit is essential to the polymer synthesis.

Conclusion

PhaC_AR possesses a wide substrate scope towards 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates. 3HB or 3HP units are essential for polymer synthesis using PhaC_AR. The presence of a 2HB monomer is key to synthesizing block copolymers, such as P(3HP)-b-P(2HB) and P(3HB-co-LMC HA)-b-P(2HB)s. The copolymers that did not contain 2HB units had a random sequence. This study’s results provide insights into the mechanism of sequence regulation by PhaC_AR and pave the way for designing PHA block copolymers., 32094019

Directed Evolution of Sequence-Regulating Polyhydroxyalkanoate Synthase to Synthesize a Medium-Chain-Length–Short-Chain-Length (MCL–SCL) Block Copolymer
Hien Thi Phan, Yumi Hosoe, Maureen Guex, Masayoshi Tomoi, Hiroya Tomita, Manfred Zinn, Ken’ichiro Matsumoto
Biomacromolecules, 23, 3, 1221, 1231, American Chemical Society (ACS), 14 Mar. 2022, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal, 32094019

Biosynthesis of poly(glycolate-co-3-hydroxybutyrate-co-3-hydroxyhexanoate) in Escherichia coli expressing sequence-regulating polyhydroxyalkanoate synthase and medium-chain-length 3-hydroxyalkanoic acid coenzyme A ligase
Hiroya Tomita, Keigo Satoh, Christopher T Nomura, Ken'ichiro Matsumoto
Bioscience, Biotechnology, and Biochemistry, 86, 2, 217, 223, Oxford University Press (OUP), 24 Jan. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, ABSTRACT

Chimeric polyhydroxyalkanoate synthase PhaCAR is characterized by the capacity to incorporate unusual glycolate (GL) units and spontaneously synthesize block copolymers. The GL and 3-hydroxybutyrate (3HB) copolymer synthesized by PhaCAR is a random-homo block copolymer, poly(GL-ran-3HB)-b-poly(3HB). In the present study, medium-chain-length 3-hydroxyhexanoate (3HHx) units were incorporated into this copolymer using PhaCAR for the first time. The coenzyme A (CoA) ligase from Pseudomonas oleovorans (AlkK) serves as a simple 3HHx-CoA supplying route in Escherichia coli from exogenously supplemented 3HHx. NMR analyses of the obtained polymers revealed that 3HHx units were randomly connected to 3HB units, whereas GL units were heterogeneously distributed. Therefore, the polymer is composed of 2 segments: P(3HB-co-3HHx) and P(GL-co-3HB-co-3HHx). The thermal and mechanical properties of the terpolymer indicate no contiguous P(3HB) segments in the material, consistent with the NMR results. Therefore, PhaCAR synthesized the novel block copolymer P(3HB-co-3HHx)-b-P(GL-co-3HB-co-3HHx), which is the first block polyhydroxyalkanoate copolymer comprising 2 copolymer segments., 32094019

Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
Yuki Kageyama, Hiroya Tomita, Takuya Isono, Toshifumi Satoh, Ken’ichiro Matsumoto
Scientific Reports, 11, 1, 22446, 22446, Springer Science and Business Media LLC, Dec. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, The first polyhydroxyalkanoate (PHA) block copolymer poly(2-hydroxybutyrate-b-3-hydroxybutyrate) [P(2HB-b-3HB)] was previously synthesized using engineered Escherichia coli expressing a chimeric PHA synthase PhaC_AR with monomer sequence-regulating capacity. In the present study, the physical properties of the block copolymer and its relevant random copolymer P(2HB-ran-3HB) were evaluated. Stress–strain tests on the P(88 mol% 2HB-b-3HB) film showed an increasing stress value during elongation up to 393%. In addition, the block copolymer film exhibited slow contraction behavior after elongation, indicating that P(2HB-b-3HB) is an elastomer-like material. In contrast, the P(92 mol% 2HB-ran-3HB) film, which was stretched up to 692% with nearly constant stress, was stretchable but not elastic. The differential scanning calorimetry and wide-angle X-ray diffraction analyses indicated that the P(2HB-b-3HB) contained the amorphous P(2HB) phase and the crystalline P(3HB) phase, whereas P(2HB-ran-3HB) was wholly amorphous. Therefore, the elasticity of P(2HB-b-3HB) can be attributed to the presence of the crystalline P(3HB) phase and a noncovalent crosslinked structure by the crystals. These results show the potential of block PHAs as elastic materials.

Evolution of polyhydroxyalkanoate synthesizing systems toward a sustainable plastic industry
Seiichi Taguchi, Ken'ichiro Matsumoto
POLYMER JOURNAL, 53, 1, 67, 79, Jan. 2021, [Peer-reviewed]
English, Scientific journal

Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli
Shuzo Arai, Sayaka Sakakibara, Robin Mareschal, Toshihiko Ooi, Manfred Zinn, Ken'ichiro Matsumoto
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 8, 612991, Dec. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal, 32094019

Isolation of poly[d-lactate (LA)-co-3-hydroxybutyrate)]-degrading bacteria from soil and characterization of d-LA homo-oligomer degradation by the isolated strains
Chiaki Hori, Tomohiro Sugiyama, Kodai Watanabe, Jian Sun, Yuu Kamada, Toshihiko Ooi, Takuya Isono, Toshifumi Satoh, Shin-ichiro Sato, Seiichi Taguchi, Ken'ichiro Matsumoto
Polymer Degradation and Stability, 179, 109231, 109231, Elsevier BV, Sep. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Synergy of valine and threonine supplementation on poly(2-hydroxybutyrate-block-3-hydroxybutyrate) synthesis in engineered Escherichia coli expressing chimeric polyhydroxyalkanoate synthase
Maho Sudo, Chiaki Hori, Toshihiko Ooi, Shoji Mizuno, Takeharu Tsuge, Ken'ichiro Matsumoto
Journal of Bioscience and Bioengineering, 129, 3, 302, 306, Elsevier BV, Mar. 2020, [Peer-reviewed], [Corresponding author], [Domestic magazines]
English, Scientific journal, The engineered chimeric polyhydroxyalkanoate (PHA) synthase PhaCAR is composed of N-terminal portion of Aeromonas caviae PHA synthase and C-terminal portion of Ralstonia eutropha (Cupriavidus necator) PHA synthase. PhaCAR has a unique and useful capacity to synthesize the block PHA copolymer poly(2-hydroxybutyrate-block-3-hydroxybutyrate) [P(2HB-b-3HB)] in engineered Escherichia coli from exogenous 2HB and 3HB. In the present study, we initially attempted to incorporate the amino acid-derived 2-hydroxyalkanoate (2HA) units using PhaCAR and the 2HA-CoA-supplying enzymes lactate dehydrogenase (LdhA) and CoA transferase (HadA). Cells harboring the genes for PhaCAR, LdhA, and HadA, as well as for the 3HB-CoA-supplying enzymes β-ketothiolase and acetoacetyl-CoA reductase, were cultivated with supplementation of four hydrophobic amino acids, i.e., leucine, valine (Val), isoleucine (Ile), and phenylalanine, in the medium. No hydrophobic amino acid-derived monomers were incorporated into the polymer, which was most likely because of the strict substrate specificity of PhaCAR; however, P(2HB-co-3HB) was unexpectedly produced with Val supplementation. The copolymer was likely P(2HB-b-3HB) based on proton nuclear magnetic resonance analysis. Based on the endogenous pathways in E. coli, 2HB units are likely derived from threonine (Thr) through deamination and dihydroxylation. In fact, dual supplementation with Thr and Val showed synergy on the 2HB fraction of the polymer. Val supplementation promoted the 2HB synthesis likely by inhibiting the metabolism of 2-ketobutyrate into Ile and/or activating Thr dehydratase. In conclusion, the LdhA/HadA/PhaCAR pathway served as the system for the synthesis of P(2HB-b-3HB) from biomass-derived carbon sources.

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli.
Matsumoto K, Saito J, Yokoo T, Hori C, Nagata A, Kudoh Y, Ooi T, Taguchi S
Journal of bioscience and bioengineering, 128, 3, 302, 306, Sep. 2019, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Increased Production and Molecular Weight of Artificial Polyhydroxyalkanoate Poly(2-hydroxybutyrate) Above the Glass Transition Temperature Threshold.
Matsumoto K, Kageyama Y
Frontiers in bioengineering and biotechnology, 7, 177, Jul. 2019, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

High-cell density culture of poly(lactate-co-3-hydroxybutyrate)-producing Escherichia coli by using glucose/xylose-switching fed-batch jar fermentation.
Chiaki Hori, Takashi Yamazaki, Greg Ribordy, Kenji Takisawa, Ken'ichiro Matsumoto, Toshihiko Ooi, Manfred Zinn, Seiichi Taguchi
Journal of bioscience and bioengineering, 127, 6, 721, 725, Jun. 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)] is produced in engineered Escherichia coli harboring the genes encoding an LA-polymerizing enzyme (LPE) and monomer-supplying enzymes. In this study, high cell-density fed-batch jar fermentation was developed using xylose and/or glucose as the carbon source. Fed-batch fermentation was initially performed with 20 g/L sugar during the batch phase for 24 h, and subsequent sugar feeding from 24 to 86 h. The feeding rate was increased in a stepwise manner. When xylose alone was used for cultivation, the cells produced the polymer at 11.6 g/L, which was higher than the 4.3 g/L obtained using glucose as the sole carbon source. However, in the first 24 h the growth in the glucose culture was greater than in the xylose culture. Based on these results, glucose was used for cell growth (at the initial stage) and xylose was used for polymer production (at the feeding stage). As expected, in the glucose/xylose switching fermentation method, polymer production was significantly enhanced, eventually reaching 26.7 g/L. The enhanced polymer production obtained by using xylose was presumably due to overflow metabolism. In fact, during xylose feeding, acetic acid excretion was greater than that in case of the glucose grown culture, suggesting the channeling of the metabolic flux from acetyl-CoA towards polymer production over into the tricarboxylic acid cycle in the xylose-fed cultures. Therefore, this sequential glucose/xylose feed strategy is potentially useful for production of acetyl-CoA derived compounds in E. coli.

Biosynthesis of novel lactate-based polymers containing medium-chain-length 3-hydroxyalkanoates by recombinant Escherichia coli strains from glucose.
Goto S, Hokamura A, Shiratsuchi H, Taguchi S, Matsumoto K, Abe H, Tanaka K, Matsusaki H
Journal of bioscience and bioengineering, 127, 7, 7, 11, Feb. 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Novel lactate (LA)-based polymers containing medium-chain-length 3-hydroxyalkanoates (MCL-3HA) were produced in fadR-deficient Escherichia coli strains from glucose as the sole carbon source. The genes encoding LA and 3-hydroxybutyrate (3HB) monomers supplying enzymes [propionyl-CoA transferase (PCT), d-lactate dehydrogenase (D-LDH), β-ketothiolase (PhaA), and NADPH-dependent acetoacetyl-CoA reductase (PhaB)], MCL-3HA monomers supplying enzymes [ (R)-3-hydroxyacyl-ACP thioesterase (PhaG) and (R)-3-hydroxyacyl (3HA)-CoA ligase] via fatty acid biosynthesis pathway, and modified polyhydroxyalkanoate (PHA) synthase [PhaC1(STQK)] of Pseudomonas sp. 61-3 were introduced into E. coli LS5218. This resulted in the synthesis of a novel LA-based copolymer, P(LA-co-3HB-co-3HA). 1H-nuclear magnetic resonance (NMR) analysis revealed the composition of P(LA-co-3HB-co-3HA) to be 19.7 mol% LA (C3), 74.9 mol% 3HB (C4), and 5.4 mol% MCL-3HA units of C8 and C10. Furthermore, the recombinant E. coli CAG18497 strain carrying these genes, excluding the phaAB genes, accumulated P(92.0% LA-co-3HA) with a novel monomer composition containing C3, C8, C10, and C12. 13C-NMR analysis showed the existence of LA-3HA sequence in the polymer. The solvent cast film of P(92.0% LA-co-3HA) exhibited transparency similar to poly(lactic acid).

Influence of Unusual Co-substrates on the Biosynthesis of Medium-Chain-Length Polyhydroxyalkanoates Produced in Multistage Chemostat.
Nils Hanik, Camila Utsunomia, Shuzo Arai, Ken'ichiro Matsumoto, Manfred Zinn
Frontiers in bioengineering and biotechnology, 7, 301, 301, 2019, [International Magazine]
English, Scientific journal, A two-stage chemostat cultivation was used to investigate the biosynthesis of functionalized medium-chain-length polyhydroxyalkanoate (mcl-PHA) in the β-oxidation weakened strain of Pseudomonas putida KTQQ20. Chemostats were linked in sequence and allowed separation of biomass production in the first stage from the PHA synthesis in the second stage. Four parallel reactors in the second stage provided identical growth conditions and ensured that the only variable was the ratio of decanoic acid (C10) to an unusual PHA monomer precursor, such as 10-undecenoic acid (C11:1) or phenylvaleric acid (PhVA). Obtained PHA content was in the range of 10 to 25 wt%. When different ratios of C10 and C11:1 were fed to P. putida, the produced PHA had a slightly higher molar ratio in favor of C11:1-based 3-hydroxy-10-undecenoate. However, in case of PhVA a significantly lower incorporation of 3-hydroxy-5-phenylvalerate over 3-hydroxydecanoate took place when compared to the ratio of their precursors in the feed medium. A result that is explained by a less efficient uptake of PhVA compared to C10 and a 24% lower yield of polymer from the aromatic fatty acid ( y P H A - M P h V A = 0.25). In addition, PHA isolated from cultivations with PhVA resulted in the number average molecular weight M n ¯ two times lower than the PHA produced from C10 alone. Detection of products from PhVA metabolism in the culture supernatant showed that uptaken PhVA was not entirely converted into PHA, thus explaining the difference in the yield polymer from substrate. It was concluded that PhVA or its related metabolites increased the chain transfer rate during PHA biosynthesis in P. putida KTQQ20, resulting in a reduction of the polymer molecular weight.

Enhancement of lactate fraction in poly(lactate-co-3-hydroxybutyrate) synthesized by Escherichia coli harboring the D-lactate dehydrogenase gene from Lactobacillus acetotolerans HT
Saki Goto, Naoyuki Suzuki, Ken'ichiro Matsumoto, Seiichi Taguchi, Kenji Tanaka, Hiromi Matsusaki
The Journal of General and Applied Microbiology, 65, 4, 204, 208, Microbiology Research Foundation, 2019, [Peer-reviewed]
Scientific journal

In Vitro Analysis of d-Lactyl-CoA-Polymerizing Polyhydroxyalkanoate Synthase in Polylactate and Poly(lactate- co-3-hydroxybutyrate) Syntheses.
Matsumoto K, Iijima M, Hori C, Utsunomia C, Ooi T, Taguchi S
Biomacromolecules, 19, 7, 2889, 2895, Jul. 2018, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Site-directed saturation mutagenesis of polyhydroxylalkanoate synthase for efficient microbial production of poly[ (R)-2-hydroxybutyrate]
Chiaki Hori, Kenta Oishi, Ken'ichiro Matsumoto, Seiichi Taguchi, Toshihiko Ooi
Journal of Bioscience and Bioengineering, 125, 6, 632, 636, Elsevier B.V., 01 Jun. 2018, [Peer-reviewed]
English, Scientific journal

Enhanced production of lactate-based polyesters in Escherichia coli from a mixture of glucose and xylose by Mlc-mediated catabolite derepression.
Ryosuke Kadoya, Ken'ichiro Matsumoto, Kenji Takisawa, Toshihiko Ooi, Seiichi Taguchi
Journal of bioscience and bioengineering, 125, 4, 365, 370, Apr. 2018, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Lignocellulose-utilizing biorefinery is a promising strategy for the sustainable production of value-added products such as bio-based polymers. Simultaneous consumption of glucose and xylose in Escherichia coli was achieved by overexpression of the gene encoding Mlc, a multiple regulator of glucose and xylose uptake. This catabolite derepression gave the enhancement in the production of poly (15 mol% lactate-co-3-hydroxybutyrate), up to 65% from 50% (wild-type strain) in the cellular contents, of the Mlc-overexpressing strain of E. coli on a mixture of glucose and xylose as carbon sources. Microscopic analysis indicated that the Mlc-overexpressing strain showed the enlargement of cell volume in the presence and absence of polymer production, consequently making an expanded volumetric space available for enhanced polymer accumulation. The enhanced polymer production by the catabolite derepression was also reproducible using the biomass, Miscanthus×giganteus (hybrid Miscanthus), which was cultivated in the farm of Hokkaido University.

Dynamic Changes of Intracellular Monomer Levels Regulate Block Sequence of Polyhydroxyalkanoates in Engineered Escherichia coli
Ken'Ichiro Matsumoto, Chiaki Hori, Ryunosuke Fujii, Masahiro Takaya, Takashi Ooba, Toshihiko Ooi, Takuya Isono, Toshifumi Satoh, Seiichi Taguchi
Biomacromolecules, 19, 2, 662, 671, 12 Feb. 2018, [Peer-reviewed], [Lead author]
English, Scientific journal

Incorporation of Glycolate Units Promotes Hydrolytic Degradation in Flexible Poly(glycolate-co-3-hydroxybutyrate) Synthesized by Engineered Escherichia coli
Ken'ichiro Matsumoto, Tetsufumi Shiba, Yukikazu Hiraide, Seiichi Taguchi
ACS BIOMATERIALS SCIENCE & ENGINEERING, 3, 12, 3058, 3063, Dec. 2017, [Peer-reviewed]
English, Scientific journal

Investigation of the Escherichia coli membrane transporters involved in the secretion of D-lactate-based oligomers by loss-of-function screening
Camila Utsunomia, Chiaki Hori, Ken'ichiro Matsumoto, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124, 6, 635, 640, Dec. 2017, [Peer-reviewed]
English, Scientific journal

Synthesis of lactate (LA)-based poly(ester-urethane) using hydroxyl-terminated LA-based oligomers from a microbial secretion system
Camila Utsunomia, Tatsuya Saito, Ken'ichiro Matsumoto, Chiaki Hori, Takuya Isono, Toshifumi Satoh, Seiichi Taguchi
JOURNAL OF POLYMER RESEARCH, 24, 10, 167, 167, Sep. 2017, [Peer-reviewed]
English, Scientific journal

In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay)
Ken'ichiro Matsumoto, Kurato Yamazaki, Shun Kawakami, Daichi Miyoshi, Toshihiko Ooi, Shigeki Hashimoto, Seiichi Taguchi
SCIENTIFIC REPORTS, 7, 12136, 12136, Sep. 2017, [Peer-reviewed]
English, Scientific journal

Microbial secretion of lactate-enriched oligomers for efficient conversion into lactide: A biological shortcut to polylactide
Camila Utsunomia, Ken'ichiro Matsumoto, Sakiko Date, Chiaki Hori, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124, 2, 204, 208, Aug. 2017, [Peer-reviewed]
English, Scientific journal

Genome-wide screening of transcription factor deletion targets in Escherichia coli for enhanced production of lactate-based polyesters
Ryosuke Kadoya, Yu Kodama, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123, 5, 535, 539, May 2017, [Peer-reviewed]
English, Scientific journal

Effect of acetate as a co-feedstock on the production of poly(lactate-co-3-hydroxyalkanoate) by pflA-deficient Escherichia coli RSC10
Lucia Salamanca-Cardona, Ryan A. Scheel, Kouhei Mizuno, N. Scott Bergey, Arthur J. Stipanovic, Ken'ichiro Matsumoto, Seiichi Taguchi, Christopher T. Nomura
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123, 5, 547, 554, May 2017, [Peer-reviewed]
English, Scientific journal

Xylose-based hydrolysate from eucalyptus extract as feedstock for poly(lactate-co-3-hydroxybutyrate) production in engineered Escherichia coli
Kenji Takisawa, Toshihiko Ooi, Ken'ichiro Matsumoto, Ryosuke Kadoya, Seiichi Taguchi
PROCESS BIOCHEMISTRY, 54, 102, 105, Mar. 2017, [Peer-reviewed]
English, Scientific journal

Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants
Takeshi Yoshizumi, Miwa Yamada, Mieko Higuchi-Takeuchi, Ken'ichiro Matsumoto, Seiichi Taguchi, Minami Matsui, Keiji Numata
PLANT BIOTECHNOLOGY, 34, 1, 39, 43, Mar. 2017, [Peer-reviewed]
English, Scientific journal

Microbial Secretion of D-Lactate-Based Oligomers
Camila Utsunomia, Ken'ichiro Matsumoto, Seiichi Taguchi
ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 5, 3, 2360, 2367, Mar. 2017, [Peer-reviewed]
English, Scientific journal

Co-crystallization phenomena in biosynthesized isotactic poly [ (R)-lactate-co-(R)-2-hydroxybutyratels with various lactate unit ratios
Taizo Kabe, Ken'ichiro Matsumoto, Satsuki Terai, Takaaki Hikima, Masaki Takata, Masahiro Miyake, Seiichi Taguchi, Tadahisa Iwata
POLYMER DEGRADATION AND STABILITY, 132, 137, 144, Oct. 2016, [Peer-reviewed]
English, Scientific journal

Consolidated bioprocessing of poly(lactate-co-3-hydroxybutyrate) from xylan as a sole feedstock by genetically-engineered Escherichia coli
Lucia Salamanca-Cardona, Ryan A. Scheel, Norman Scott Bergey, Arthur J. Stipanovic, Ken'ichiro Matsumoto, Seiichi Taguchi, Christopher T. Nomura
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122, 4, 406, 414, Oct. 2016, [Peer-reviewed]
English, Scientific journal

Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars
Jian Sun, Camila Utsunomia, Shohei Sasaki, Ken'ichiro Matsumoto, Toshihiko Yamada, Toshihiko Ooi, Seiichi Taguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80, 4, 818, 820, Apr. 2016, [Peer-reviewed]
English, Scientific journal

Molecular weight-dependent degradation of D-lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp C34 and Alcaligenes faecalis T1
Jian Sun, Ken'ichiro Matsumoto, Yuta Tabata, Ryosuke Kadoya, Toshihiko Ooi, Hideki Abe, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99, 22, 9555, 9563, Nov. 2015, [Peer-reviewed]
English, Scientific journal

Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli
Ryosuke Kadoya, Yu Kodama, Ken'ichiro Matsumoto, Seiichi Taguchi
BIOENGINEERED, 6, 5, 307, 311, Sep. 2015, [Peer-reviewed]
English, Scientific journal

Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase
Toshinori Yokoo, Ken'ichiro Matsumoto, Takashi Ooba, Kenjiro Morimoto, Seiichi Taguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79, 6, 986, 988, Jun. 2015, [Peer-reviewed]
English, Scientific journal

MtgA Deletion-Triggered Cell Enlargement of Escherichia coli for Enhanced Intracellular Polyester Accumulation
Ryosuke Kadoya, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
PLOS ONE, 10, 6, e0125163, e0125163, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Enhanced cellular content and lactate fraction of the poly(lactate-co-3-hydroxybutyrate) polyester produced in recombinant Escherichia coli by the deletion of sigma factor RpoN
Ryosuke Kadoya, Yu Kodama, Ken'ichiro Matsumoto, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119, 4, 427, 429, Apr. 2015, [Peer-reviewed]
English, Scientific journal

Enzymatic characterization of a depolymerase from the isolated bacterium Variovorax sp. C34 that degrades poly(enriched lactate-co-3-hydroxybutyrate)
Jian Sun, Ken'ichiro Matsumoto, John Masani Nduko, Toshihiko Ooi, Seiichi Taguchi
Polymer Degradation and Stability, 110, 44, 49, Elsevier BV, Dec. 2014, [Peer-reviewed]
Scientific journal

Improved production of poly(lactic acid)-like polyester based on metabolite analysis to address the rate-limiting step
Ken'ichiro Matsumoto, Kota Tobitani, Shunsuke Aoki, Yuyang Song, Toshihiko Ooi, Seiichi Taguchi
AMB EXPRESS, 4, 1, 1, 5, Nov. 2014, [Peer-reviewed]
English, Scientific journal

Engineering Escherichia coli for Improved Production of Short-Chain-Length-co-Medium-Chain-Length Poly[ (R)-3-hydroxyalkanoate] (SCL-co-MCL PHA) Copolymers from Renewable Nonfatty Acid Feedstocks
Ryan C. Tappel, Wenyang Pan, N. Scott Bergey, Qin Wang, Ivory L. Patterson, Obiajulu A. Ozumba, Ken'ichiro Matsumoto, Seiichi Taguchi, Christopher T. Nomura
ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 2, 7, 1879, 1887, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Deletion of the pflA gene in Escherichia coli LS5218 and its effects on the production of polyhydroxyalkanoates using beechwood xylan as a feedstock
Lucia Salamanca-Cardona, Ryan A. Scheel, Benjamin R. Lundgren, Arthur J. Stipanovic, Ken’ichiro Matsumoto, Seiichi Taguchi, Christopher T. Nomura
Bioengineered, 5, 5, 284, 287, 20 Jun. 2014, [Peer-reviewed]
English, Scientific journal

Enhanced production of poly(lactate-co-3-hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter
John Masani Nduko, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98, 6, 2453, 2460, Mar. 2014, [Peer-reviewed]
English, Scientific journal

Enzyme and metabolic engineering for the production of novel biopolymers: crossover of biological and chemical processes
Ken'ichiro Matsumoto, Seiichi Taguchi
CURRENT OPINION IN BIOTECHNOLOGY, 24, 6, 1054, 1060, Dec. 2013, [Peer-reviewed]
English, Scientific journal

Biosynthetic polyesters consisting of 2-hydroxyalkanoic acids: current challenges and unresolved questions
Ken'ichiro Matsumoto, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97, 18, 8011, 8021, Sep. 2013, [Peer-reviewed]
English, Scientific journal

One-Pot Microbial Production, Mechanical Properties, and Enzymatic Degradation of Isotactic P[ (R)-2-hydroxybutyrate] and Its Copolymer with (R)-Lactate
Ken'ichiro Matsumoto, Satsuki Terai, Ayako Ishiyama, Jian Sun, Taizo Kabe, Yuyang Song, John Masani Nduko, Tadahisa Iwata, Seiichi Taguchi
BIOMACROMOLECULES, 14, 6, 1913, 1918, Jun. 2013, [Peer-reviewed]
English, Scientific journal

Engineering of class i lactate-polymerizing polyhydroxyalkanoate synthases from Ralstonia eutropha that synthesize lactate-based polyester with a block nature
Anna Ochi, Ken'Ichiro Matsumoto, Takashi Ooba, Kohei Sakai, Takeharu Tsuge, Seiichi Taguchi
Applied Microbiology and Biotechnology, 97, 8, 3441, 3447, Apr. 2013, [Peer-reviewed]
English, Scientific journal

Efficient (R)-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase
Ken'ichiro Matsumoto, Takehiro Okei, Inori Honma, Toshihiko Ooi, Hirobumi Aoki, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97, 1, 205, 210, Jan. 2013, [Peer-reviewed]
English, Scientific journal

Single-step production of polyhydroxybutyrate from starch by using alpha-amylase cell-surface displaying system of Corynebacterium glutamicum
Yuyang Song, Ken'ichiro Matsumoto, Tsutomu Tanaka, Akihiko Kondo, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115, 1, 12, 14, Jan. 2013, [Peer-reviewed]
English, Scientific journal

Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme
John Masani Nduko, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
METABOLIC ENGINEERING, 15, 159, 166, Jan. 2013, [Peer-reviewed]
English, Scientific journal

Directed evolution and structural analysis of nadph-dependent acetoacetyl coenzyme A(acetoacetyl-CoA) reductase from ralstonia eutropha reveals two mutations responsible for enhanced kinetics
Ken'ichiro Matsumoto, Yoshikazu Tanaka, Tsuyoshi Watanabe, Ren Motohashi, Koji Ikeda, Kota Tobitani, Min Yao, Isao Tanaka, Seiichi Taguchi
Applied and Environmental Microbiology, 79, 19, 6134, 6139, 19, 2013, [Peer-reviewed]
English, Scientific journal

Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production
Yuyang Song, Ken'ichiro Matsumoto, Miwa Yamada, Aoi Gohda, Christopher J. Brigham, Anthony J. Sinskey, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 93, 5, 1917, 1925, Mar. 2012, [Peer-reviewed]
English, Scientific journal

Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural
John Masani Nduko, Wakako Suzuki, Ken'ichiro Matsumoto, Hirokazu Kobayashi, Toshihiko Ooi, Atsushi Fukuoka, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113, 1, 70, 72, Jan. 2012, [Peer-reviewed]
English, Scientific journal

Biological Lactate-Polymers Synthesized by One-Pot Microbial Factory: Enzyme and Metabolic Engineering
John Masani Nduko, Ken'ichiro Matsumoto, Seiichi Taguchi
BIOBASED MONOMERS, POLYMERS, AND MATERIALS, 1105, 213, 235, 2012, [Peer-reviewed]
English, International conference proceedings

Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase
Ken'ichiro Matsumoto, Ayako Ishiyama, Kohei Sakai, Tetsufumi Shiba, Seiichi Taguchi
JOURNAL OF BIOTECHNOLOGY, 156, 3, 214, 217, Dec. 2011, [Peer-reviewed]
English, Scientific journal

Chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA) incorporating 2-hydroxybutyrate by wild-type class I PHA synthase from Ralstonia eutropha
Xuerong Han, Yasuharu Satoh, Toshifumi Satoh, Ken'ichiro Matsumoto, Toyoji Kakuchi, Seiichi Taguchi, Tohru Dairi, Masanobu Munekata, Kenji Tajima
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92, 3, 509, 517, Nov. 2011, [Peer-reviewed]
English, Scientific journal

Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli
Miwa Yamada, Ken'ichiro Matsumoto, Shu Uramoto, Ren Motohashi, Hideki Abe, Seiichi Taguchi
JOURNAL OF BIOTECHNOLOGY, 154, 4, 255, 260, Jul. 2011, [Peer-reviewed]
English, Scientific journal

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor
Ken'ichiro Matsumoto, Kei Kitagawa, Sung-Jin Jo, Yuyang Song, Seiichi Taguchi
JOURNAL OF BIOTECHNOLOGY, 152, 4, 144, 146, Apr. 2011, [Peer-reviewed]
English, Scientific journal

Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes
Ken'ichiro Matsumoto, Kenjiro Morimoto, Aoi Gohda, Hiroald Shimada, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111, 4, 485, 488, Apr. 2011, [Peer-reviewed]
English, Scientific journal

Biosynthesis of a lactate (LA)-based polyester with a 96 mol% la fraction and its application to stereocomplex formation
Fumi Shozui, Ken'Ichiro Matsumoto, Ren Motohashi, Jian Sun, Toshifumi Satoh, Toyoji Kakuchi, Seiichi Taguchi
Polymer Degradation and Stability, 96, 4, 499, 504, Apr. 2011, [Peer-reviewed]
English, Scientific journal

Chemo-microbial conversion of cellulose into polyhydroxybutyrate through ruthenium-catalyzed hydrolysis of cellulose into glucose
Ken'ichiro Matsumoto, Hirokazu Kobayashi, Koji Ikeda, Tasuku Komanoya, Atsushi Fukuoka, Seiichi Taguchi
BIORESOURCE TECHNOLOGY, 102, 3, 3564, 3567, Feb. 2011, [Peer-reviewed]
English, Scientific journal

A New Pathway for Poly(3-hydroxybutyrate) Production in Escherichia coli and Corynebacterium glutamicum by Functional Expression of a New Acetoacetyl-coenzyme A Synthase
Ken'ichiro Matsumoto, Miwa Yamada, Chean Ring Leong, Sung-Jin Jo, Tomohisa Kuzuyama, Seiichi Taguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75, 2, 364, 366, Feb. 2011, [Peer-reviewed]
English, Scientific journal

A Novel Factor FLOURY ENDOSPERM2 Is Involved in Regulation of Rice Grain Size and Starch Quality
Kao-Chih She, Hiroaki Kusano, Kazuyoshi Koizumi, Hiromoto Yamakawa, Makoto Hakata, Tomohiro Imamura, Masato Fukuda, Natsuka Naito, Yumi Tsurumaki, Mitsuhiro Yaeshima, Tomohiko Tsuge, Ken'ichiro Matsumoto, Mari Kudoh, Eiko Itoh, Shoshi Kikuchi, Naoki Kishimoto, Junshi Yazaki, Tsuyu Ando, Masahiro Yano, Takashi Aoyama, Tadamasa Sasaki, Hikaru Satoh, Hiroaki Shimada
PLANT CELL, 22, 10, 3280, 3294, Oct. 2010, [Peer-reviewed]
English, Scientific journal

Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) Terpolymers Using a Chimeric PHA Synthase in Recombinant Ralstonia eutropha and Pseudomonas putida
Jian Sun, Fumi Shozui, Miwa Yamada, Ken'ichiro Matsumoto, Kazuma Takase, Seiichi Taguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74, 8, 1716, 1718, Aug. 2010, [Peer-reviewed]
English, Scientific journal

A New Beneficial Mutation in Pseudomonas sp 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template
Fumi Shozui, Jian Sun, Yuyang Song, Miwa Yamada, Kohei Sakai, Ken'ichiro Matsumoto, Kazuma Takase, Seiichi Taguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74, 8, 1710, 1712, Aug. 2010, [Peer-reviewed]
English, Scientific journal

Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction of beta-oxidation in Escherichia coli LS5218
Fumi Shozui, Ken'ichiro Matsumoto, Ren Motohashi, Miwa Yamada, Seiichi Taguchi
POLYMER DEGRADATION AND STABILITY, 95, 8, 1340, 1344, Aug. 2010, [Peer-reviewed]
English, Scientific journal

Quick and efficient method for genetic transformation of biopolymer-producing bacteria
Qin Wang, Alexander P. Mueller, Chean Ring Leong, Ken'ichiro Matsumoto, Seiichi Taguchi, Christopher T. Nomura
Journal of Chemical Technology and Biotechnology, 85, 6, 775, 778, Jun. 2010, [Peer-reviewed]
English, Scientific journal

Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus
Ken'ichiro Matsumoto, Yuichi Mukai, Daiki Ogata, Fumi Shozui, John Masani Nduko, Seiichi Taguchi, Toshihiko Ooi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 86, 5, 1431, 1438, May 2010, [Peer-reviewed]
English, Scientific journal

Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides: Case study on engineered apidaecins
Ken'ichiro Matsumoto, Yoshitake Orikasa, Kenta Ichinohe, Shigeki Hashimoto, Toshihiko Ooi, Seiichi Taguchi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 395, 1, 7, 10, Apr. 2010, [Peer-reviewed]
English, Scientific journal

Adjustable Mutations in Lactate (LA)-Polymerizing Enzyme for the Microbial Production of LA-Based Polyesters with Tailor-Made Monomer Composition
Miwa Yamada, Ken'ichiro Matsumoto, Kotaro Shimizu, Shu Uramoto, Takanori Nakai, Fumi Shozui, Seiichi Taguchi
BIOMACROMOLECULES, 11, 3, 815, 819, Mar. 2010, [Peer-reviewed]
English, Scientific journal

Biosynthesis of novel terpolymers poly(lactate-co-3-hydroxybutyrate-co-3-hydroxyvalerate)s in lactate-overproducing mutant Escherichia coli JW0885 by feeding propionate as a precursor of 3-hydroxyvalerate
Fumi Shozui, Ken'ichiro Matsumoto, Takanori Nakai, Miwa Yamada, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85, 4, 949, 954, Jan. 2010, [Peer-reviewed]
English, Scientific journal

Enzymatic and whole-cell synthesis of lactate-containing polyesters: toward the complete biological production of polylactate
Ken'ichiro Matsumoto, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85, 4, 921, 932, Jan. 2010, [Peer-reviewed]
English, Scientific journal

A unique post-translational processing of an exo-beta-1,3-glucanase of Penicillium sp KH10 expressed in Aspergillus oryzae
Toshihiko Ooi, Hirokazu Sato, Ken'ichiro Matsumoto, Seiichi Taguchi
PROTEIN EXPRESSION AND PURIFICATION, 67, 2, 126, 131, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Engineering of polyhydroxyalkanoate synthase by Ser477X/Gln481X saturation mutagenesis for efficient production of 3-hydroxybutyrate-based copolyesters
Fumi Shozui, Ken'ichiro Matsumoto, Takahiro Sasaki, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84, 6, 1117, 1124, Oct. 2009, [Peer-reviewed]
English, Scientific journal

The Hydrophobicity in a Chemically Modified Side-Chain of Cysteine Residues of Thanatin Is Related to Antimicrobial Activity against Micrococcus luteus
Yoshitake Orikasa, Kenta Ichinohe, Junki Saito, Shigeki Hashimoto, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73, 7, 1683, 1684, Jul. 2009, [Peer-reviewed]
English, Scientific journal

A novel superior factor widely controlling the rice grain quality
Kao-Chih She, Hiroaki Kusano, Kazuyoshi Koizumi, Hiromoto Yamakawa, Makoto Hakata, Tomohiro Imamura, Masato Fukuda, Natsuka Naito, Yumi Tsurumaki, Ken'ichiro Matsumoto, Mari Kudoh, Eiko Itoh, Shoshi Kikuchi, Naoki Kishimoto, Junshi Yazaki, Tsuyu Ando, Masahiro Yano, Takashi Aoyama, Tadamasa Sasaki, Hikaru Satoh, Hiroaki Shimada
Nature Precedings, Springer Science and Business Media LLC, 26 May 2009
Scientific journal, Synthesis of storage starch and protein accumulation is the main action of endosperm organogenesis in term of the economic importance of rice. This event is strongly disturbed by abiotic stresses such as high temperature; thus, the upcoming global warming will cause a crisis with a great impact on food production^1,2^. The enzymes for the protein storage and starch synthesis pathway should work in concert to carry out the organogenesis of rice endosperm^3-5^, but the regulatory mechanism is largely unknown. Here we show that a novel regulatory factor, named OsCEO1, acts as the conductor of endosperm organogenesis during the rice grain filling stage. The physiological properties of floury-endosperm-2 (flo2) mutants showed many similarities to symptoms of grains developed under high-temperature conditions, suggesting important roles of the responsible gene in sensitivity to high-temperature stress. Our map-based cloning identified the responsible gene for the flo2 mutant, OsCEO1, which has no homology to any genes of known function. The OsCEO1 belongs to a novel conserved gene family and encodes a protein composed of 1,720 amino acid residues containing a TPR (tetratricopeptide repeat) motif, which is considered to mediate a protein-protein interaction. The yeast two-hybrid analysis raised an unknown protein showing homology to a late embryogenesis abundant protein and a putative basic helix-loop-helix protein as candidates for the direct interactor for OsCEO1, whereas no enzyme genes for the synthesis of storage substances were detected. The flo2 mutant exhibited reduced expression of several genes for putative regulatory proteins as well as many enzymes involved in storage starch and proteins. These results suggest that OsCEO1 is a superior conductor of the novel regulatory cascade of endosperm organogenesis and may have important roles in the response to high-temperature stress.

Comparative Enzymatic Analysis of Azoreductases from Bacillus sp B29
Toshihiko Ooi, Takeshi Shibata, Ken'ichiro Matsumoto, Shinichi Kinoshita, Seiichi Taguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73, 5, 1209, 1211, May 2009, [Peer-reviewed]
English, Scientific journal

Chimeric Enzyme Composed of Polyhydroxyalkanoate (PHA) Synthases from Ralstonia eutropha and Aeromonas caviae Enhances Production of PHAs in Recombinant Escherichia coli
Ken'ichiro Matsumoto, Kazuma Takase, Yoko Yamamoto, Yoshiharu Doi, Seiichi Taguchi
BIOMACROMOLECULES, 10, 4, 682, 685, Apr. 2009, [Peer-reviewed]
English, Scientific journal

Microbial Production of Lactate-Enriched Poly[ (R)-lactate-co-(R)-3-hydroxybutyrate] with Novel Thermal Properties
Miwa Yamada, Ken'ichiro Matsumoto, Takanori Nakai, Seiichi Taguchi
BIOMACROMOLECULES, 10, 4, 677, 681, Apr. 2009, [Peer-reviewed]
English, Scientific journal

Production of Short-Chain-Length/Medium-Chain-Length Polyhydroxyalkanoate (PHA) Copolymer in the Plastid of Arabidopsis thaliana Using an Engineered 3-Ketoacyl-acyl Carrier Protein Synthase III
Ken'ichiro Matsumoto, Takaaki Murata, Rina Nagao, Christopher T. Nomura, Satoshi Arai, Yuko Arai, Kazuma Takase, Hideo Nakashita, Seiichi Taguchi, Hiroaki Shimada
BIOMACROMOLECULES, 10, 4, 686, 690, Apr. 2009, [Peer-reviewed]
English, Scientific journal

Dual production of poly (3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum
Sung-Jin Jo, Chean Ring Leong, Ken'ichiro Matsumoto, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 107, 4, 409, 411, Apr. 2009, [Peer-reviewed]
English, Scientific journal

Kinetic Analysis of Engineered Polyhydroxyalkanoate Synthases with Broad Substrate Specificity
Ken'ichiro Matsumoto, Fumi Shozui, Yasuharu Satoh, Kenji Tajima, Masanobu Munekata, Seiichi Taguchi
POLYMER JOURNAL, 41, 3, 237, 240, 2009
English, Scientific journal

A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme
Seiichi Taguchi, Miwa Yamadaa, Ken'ichiro Matsumoto, Kenji Tajima, Yasuharu Satoh, Masanobu Munekata, Katsuhiro Ohno, Katsunori Kohda, Takashi Shimamura, Hiromi Kambe, Shusei Obata
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105, 45, 17323, 17327, Nov. 2008, [Peer-reviewed]
English, Scientific journal

FabG mediates polyhydroxyalkanoate production from both related and nonrelated carbon sources in recombinant Escherichia coli LS5218
Christopher T. Nomura, Tomoyo Tanaka, Tenai E. Eguen, Alexandria S. Appah, Ken'ichiro Matsumoto, Seiichi Taguchi, C. Leo Ortiz, Yoshiharu Doi
BIOTECHNOLOGY PROGRESS, 24, 2, 342, 351, Mar. 2008, [Peer-reviewed]
English, Scientific journal

Improvement of poly(3-hydroxybutyrate) [P(3HB)] production in Corynebacterium glutamicum by codon optimization, point mutation and gene dosage of P(3HB) biosynthetic genes
Sung-Jin Jo, Ken'ichiro Matsumoto, Chean Ring Leong, Toshihiko Ooi, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104, 6, 457, 463, Dec. 2007, [Peer-reviewed]
English, Scientific journal

Synthesis of short-chain-length/medium-chain-length polyhydroxyalkanoate (PHA) copolymers in peroxisome of the transgenic Arabidopsis thaliana harboring the PHA synthase gene from Pseudomonas sp 61-3
Ken'ichiro Matsumoto, Yuko Arai, Rina Nagao, Takaaki Murata, Kazuma Takase, Hideo Nakashita, Seiichi Taguchi, Hiroaki Shimada, Yoshiharu Doi
JOURNAL OF POLYMERS AND THE ENVIRONMENT, 14, 4, 369, 374, Oct. 2006, [Peer-reviewed]
English, Scientific journal

In vivo and in vitro characterization of Ser477X mutations in polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp 61-3: Effects of beneficial mutations on enzymatic activity, substrate specificity, and molecular weight of PHA
Ken'ichiro Matsumoto, Emi Aoki, Kazuma Takase, Yoshiharu Doi, Seiichi Taguchi
BIOMACROMOLECULES, 7, 8, 2436, 2442, Aug. 2006, [Peer-reviewed]
English, Scientific journal

Enhancement of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production in the transgenic Arabidopsis thaliana by the in vitro evolved highly active mutants of polyhydroxyalkanoate (PHA) synthase from Aeromonas caviae
K Matsumoto, R Nagao, T Murata, Y Arai, T Kichise, H Nakashita, S Taguchi, H Shimada, Y Doi
BIOMACROMOLECULES, 6, 4, 2126, 2130, Jul. 2005, [Peer-reviewed]
English, Scientific journal

Synergistic effects of Glu130Asp substitution in the type II polyhydroxyalkanoate (PHA) synthase: Enhancement of PHA production and alteration of polymer molecular weight
K Matsumoto, K Takase, E Aoki, Y Doi, S Taguchi
BIOMACROMOLECULES, 6, 1, 99, 104, Jan. 2005, [Peer-reviewed]
English, Scientific journal

Alteration of substrate chain-length specificity of type II synthase for polyhydroxyalkanoate biosynthesis by in vitro evolution: in vivo and in vitro enzyme assays
K Takase, K Matsumoto, S Taguchi, Y Doi
BIOMACROMOLECULES, 5, 2, 480, 485, Mar. 2004, [Peer-reviewed]
English, Scientific journal

Biosynthesis of biodegradable polyester: Current research and future direction
K Matsumoto, Y Doi
JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN, 61, 5, 489, 495, May 2003, [Peer-reviewed]
Japanese, Scientific journal

Biosynthesis of biodegradable polyesters from renewable carbon sources by recombinant bacteria
S Taguchi, H Matsusaki, K Matsumoto, K Takase, K Taguchi, Y Doi
POLYMER INTERNATIONAL, 51, 10, 899, 906, Oct. 2002
English, Scientific journal

Isolation and characterization of polyhydroxyalkanoates inclusions and their associated proteins in Pseudomonas sp 61-3
K Matsumoto, H Matsusaki, K Taguchi, M Seki, Y Doi
BIOMACROMOLECULES, 3, 4, 787, 792, Jul. 2002, [Peer-reviewed]
English, Scientific journal

Direct observation of polyhydroxyalkanoate chains by atomic force microscopy
K Sudesh, Z Gan, K Matsumoto, Y Doi
ULTRAMICROSCOPY, 91, 1-4, 157, 164, May 2002, [Peer-reviewed]
English, Scientific journal

Poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) copolymer from sugars by recombinant Ralstonia eutropha harboring the phaC1Ps and the phaGPs genes of Pseudomonas sp. 61-3
K. Matsumoto, S. Nakae, K. Taguchi, H. Matsusaki, M. Seki, Y. Doi
Biomacromolecules, 2, 3, 934, 939, 3, 2001, [Peer-reviewed]
English, Scientific journal

Cloning and characterization of the pseudomonas sp. 61-3 phaG gene involved in polyhydroxyalkanoate biosynthesis
K. Matsumoto, H. Matsusaki, S. Taguchi, M. Seki, Y. Doi
Biomacromolecules, 2, 1, 142, 147, 1, 2001, [Peer-reviewed]
English, Scientific journal

Real-time NMR Analysis of Polyhydroxyalkanoate Synthase Reaction That Synthesizes Block Copolymer of Glycolate and 3-Hydroxybutyrate
Yanagawa Kengo, Kajikawa Ayaka, Sakakibara Sayaka, Kumeta Hiroyuki, Tomita Hiroya, Matsumoto Ken’ichiro, Journal of Environmental Biotechnology, 23, 1, 39, 43, 2023
Japan Sciety for Environmental Biotechnology, Japanese

Real-time NMR Analysis of Polyhydroxyalkanoate Synthase Reaction That Synthesizes Block Copolymer of Glycolate and 3-Hydroxybutyrate
柳川謙吾, 梶川彩香, 榊原早也果, 久米田博之, 冨田宏矢, 冨田宏矢, 松本謙一郎, Journal of Environmental Biotechnology (Web), 23, 1, 2023

ブロック型微生物ポリエステルの生合成と物性解析
松本 謙一郎, 生物工学会誌, 100, 9, 489, 493, 25 Sep. 2022
公益社団法人 日本生物工学会, Japanese

バイオプラスチックPHAのブロック共重合体合成酵素の進化工学的機能拡張
松本 謙一郎, バイオサイエンスとインダストリー / バイオサイエンスとインダストリー編集委員会 編, 80, 5, 394, 396, 2022
バイオインダストリー協会, Japanese

Basic Knowledge for Utilization of Biobased and/or Biodegradable Plastics
松本謙一郎, 塗装工学, 56, 1, 2021

生分解性プラスチックの積極的な活用に向けて
松本謙一郎, グリーンテクノ情報, 15, 4, 2020

配列制御型ポリエステル生合成系の開発
松本謙一郎, バイオサイエンスとインダストリー, 77, 3, 2019

LC-MSによる酵素活性測定法
松本謙一郎, 生物工学会誌, 97, 8, 505, 505, 2019
(公社)日本生物工学会, Japanese

Shortcut process development of polylactide production inspired by discovery of lactate oligomers secretion
Ken'ichiro MATSUMOTO, Ayaka HIROE, Seiichi TAGUCHI, Bioscience and Industry(Japan Bioindustry Association), 76, 6, 462, 466, 2018
バイオインダストリー協会, Japanese, Introduction research institution

Effect of monomeric composition on the thermal, mechanical and crystalline properties of poly[ (R)-lactate-co-(R)-3-hydroxybutyrate]
Daisuke Ishii, Kenji Takisawa, Ken'ichiro Matsumoto, Toshihiko Ooi, Takaaki Hikima, Masaki Takata, Seiichi Taguchi, Tadahisa Iwata, POLYMER, 122, 169, 173, Jul. 2017
English

Front-Line Polymer Science : Polymers Synthesized from Plant Biomass
大井俊彦, 松本謙一郎, 田口精一, 高分子, 66, 6, 296, 300, 2017
高分子学会, Japanese, Introduction research institution

乳酸ベースバイオプラスチックの創製と「風船型」微生物工場の誕生 (コンバーティングとホワイトバイオ)
門屋 享介, 滝沢 憲治, 松本 謙一郎, 大井 俊彦, 田口 精一, コンバーテック, 44, 4, 125, 127, Apr. 2016
加工技術研究会, Japanese, Introduction research institution

合成生物学的手法により創製した多元ポリ乳酸の物性および生分解性
門屋亨介, 門屋亨介, 滝沢憲治, 滝沢憲治, USTUNOMIA Camila, 石井大輔, 石井大輔, 松本謙一郎, 松本謙一郎, 大井俊彦, 大井俊彦, 岩田忠久, 岩田忠久, 田口精一, 田口精一, 高分子学会予稿集(CD-ROM), 65, 2, 2016

酵素進化工学による高活性化ポリ(2-ヒドロキシ酪酸)[P(2HB)]重合酵素の創出
大石健太, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一, 日本農芸化学会北海道支部講演会講演要旨, 2016, 2016

キシロースを炭素源とした乳酸ポリマーを生産する組換え大腸菌の高密度培養
山崎貴史, RIBORDY Greg, 松本謙一郎, 大井俊彦, 大井俊彦, ZINN Manfred, 田口精一, 田口精一, 日本農芸化学会北海道支部講演会講演要旨, 2016, 2016

Production of poly(lactate-co-hydroxybutyrate) from Miscanthus-derived sugars
UTSUNOMIA Camila, SUN Jian, MATSUMOTO Ken’ichiro, OOI Toshihiko, OOI Toshihiko, TAGUCHI Seiichi, TAGUCHI Seiichi, 日本農芸化学会大会講演要旨集(Web), 2015, 2015

Characterization of the substrate specificity of a poly(enriched lactate-co-3-hydroxybutyrate) depolymerase
SUN Jian, MATSUMOTO Ken’ichiro, KADOYA Ryosuke, TABATA Yuta, TABATA Yuta, OOI Toshihiko, OOI Toshihiko, ABE Hideki, ABE Hideki, TAGUCHI Seiichi, TAGUCHI Seiichi, 日本農芸化学会大会講演要旨集(Web), 2015, 2015

Miscanthus × giganteus hydrolysate as the carbon source for the microbial production of poly(lactate-co-3-hydroxybutyrate)
UTSUNOMIA Camila, SUN Jian, MATSUMOTO Ken’ichiro, OOI Toshihiko, OOI Toshihiko, TAGUCHI Seiichi, TAGUCHI Seiichi, 日本農芸化学会北海道支部講演会講演要旨, 2015, 2015

ゲノム変異導入法を用いた資源循環型微生物生産ポリマー高生産化株の創成
門屋亨介, 門屋亨介, 松本謙一郎, 大井俊彦, 田口精一, 田口精一, 日本農芸化学会北海道支部講演会講演要旨, 2015, 2015

2P-174 The creation of high efficiency bioplastic producing host strain by genome engineering
Kadoya Ryosuke, Matsumoto Ken'ichiro, Ooi Toshihiko, Taguchi Seiichi, 日本生物工学会大会講演要旨集, 67, 218, 218, 2015
日本生物工学会, Japanese

2P-175 Degradation mechanism of poly(lactate-co-3-hydroxybutyrate) by depolymerase from soil bacterium Variovorax sp. C34
Camila Utsunomia, Jian Sun, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi, 日本生物工学会大会講演要旨集, 67, 218, 218, 2015
日本生物工学会, English

2P-176 Production of lactate-base polymer from herbaceous plant biomass
Ooi Toshihiko, Utsunomia Camila, Matsumoto Ken'ichiro, Taguchi Seiichi, 日本生物工学会大会講演要旨集, 67, 218, 218, 2015
日本生物工学会, Japanese

乳酸ベースバイオポリマー生産のための最適培養条件の検討
滝沢憲治, 大井俊彦, 松本謙一郎, 田口精一, 日本農芸化学会北海道支部講演会講演要旨, 2015, 2015

高分子量2‐ヒドロキシブタン酸ベースポリマーの生合成および物性解析
TAKAYA MASAHIRO, OBA TAKASHI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 66th, 68, 68, 05 Aug. 2014
日本生物工学会, Japanese

ゲノム工学的手法を用いた微生物ポリマー高生産化宿主の創成
KADOYA RYOSUKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 66th, 67, 67, 05 Aug. 2014
日本生物工学会, Japanese

二酸化炭素からプラスチックを合成する人工生命システムの創製―最短の合成ルートは?―
MATSUMOTO KEN'ICHIRO, YOKOYAMA TOSHINORI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 66th, 16, 16, 05 Aug. 2014
日本生物工学会, Japanese

グリコール酸ポリマーの生合成経路の構築
YOKOO TOSHINORI, HIRAIDE YUKIKAZU, TAGUCHI SEIICHI, MATSUMOTO, KEN'ICHIRO, 日本生物工学会大会講演要旨集, 66th, 68, 68, 05 Aug. 2014
日本生物工学会, Japanese

乳酸ポリマー合成コリネ菌の代謝解析に基づく高生産化
TOBITANI KOTA, SASAMORI TETSUYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 66th, 67, 67, 05 Aug. 2014
日本生物工学会, Japanese

Lactobacillus acetotolerans HTの乳酸脱水素酵素遺伝子を利用した乳酸ユニットを含む生分解性プラスチックの生合成
GOTO SAKI, SATO MISAKI, TANAKA KENJI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, MATSUSAKI HIROMI, 日本生物工学会大会講演要旨集, 66th, 78, 78, 05 Aug. 2014
日本生物工学会, Japanese

進化工学的手法によるポリヒドロキシアルカン酸合成酵素の乳酸重合能力の強化
AOKI SHUNSUKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 66th, 67, 67, 05 Aug. 2014
日本生物工学会, Japanese

生合成乳酸ベースポリマーの高生産化を目指した宿主大腸菌の遺伝子改変
KODAMA YU, KADOYA RYOSUKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 66th, 66, 66, 05 Aug. 2014
日本生物工学会, Japanese

組換え大腸菌を用いたグリコール酸ベースポリマーの生合成と酵素分解性
HIRAIDE YUKIKAZU, SHIBA TETSUFUMI, SU JIAN, OI TOSHIHIKO, TAGUCHI SEIICHI, MATSUMOTO, KEN'ICHIRO, 日本生物工学会大会講演要旨集, 66th, 67, 67, 05 Aug. 2014
日本生物工学会, Japanese

微生物産生ポリ[ (R)‐ラクテート‐co‐(R)‐2‐ヒドロキシブチレート]の結晶性および熱的性質
KABE TAIZO, IWATA TADAHISA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, MARUBAYASHI HIRONORI, HIKIMA TAKAAKI, TAKATA MASAKI, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.2K21, 09 May 2014
Japanese

バイオマスから高効率で乳酸プラスチックを生産する微生物工場の開発とポリマー物性評価
MATSUMOTO KEN'ICHIRO, MAEDA MICHIHISA, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2014, 4SY03-6 (WEB ONLY), 05 Mar. 2014
Japanese

微生物を利用したキラルP(2‐ヒドロキシ酪酸)の合成とポリマー解析
MIYAKE MASAHIRO, MATSUMOTO KEN'ICHIRO, TERAI SATSUKI, KABE TAIZO, IWATA TADAHISA, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2014, 2A01P07 (WEB ONLY), 05 Mar. 2014
Japanese

植物バイオマス由来の単糖混合物を炭素源としたバイオポリエステルの生産
OI TOSHIHIKO, SASAKI SHOHEI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2014, 2A01P08 (WEB ONLY), 05 Mar. 2014
Japanese

微生物産生ポリエステルの熱分解特性と紡糸条件に関する研究
加部泰三, 加部泰三, 加部泰三, 松本謙一郎, 田口精一, 田口精一, 柘植丈晴, 柘植丈晴, 引間孝明, 高田昌樹, 岩田忠久, 岩田忠久, 岩田忠久, 高分子学会予稿集(CD-ROM), 63, 2, 2014

1P-202 Enzymatic characterization of a polymer degrading enzyme purified from an isolated bacterium :
Jian Sun, John Nduko, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi, 日本生物工学会大会講演要旨集, 66, 68, 68, 2014
日本生物工学会, English

2S-Ba02 Microbial plastic factory driven by renewable carbon sources :
Seiichi Taguchi, Ken'ichiro Matsumoto, 日本生物工学会大会講演要旨集, 66, 85, 85, 2014
日本生物工学会, English

ポリ[ (R)‐2‐ヒドロキシブチレート]の基礎物性評価と構造解析の試み
MAKINO KYOHEI, KABE TAIZO, TAKEMURA AKIO, IWATA TADAHISA, TAGUCHI SEIICHI, MATSUMOTO KEN'ICHIRO, HIKIMA TAKAAKI, TAKATA MASAKI, MARUBAYASHI HIRONORI, 繊維学会予稿集, 69, 1 (CD-ROM), ROMBUNNO.2P250, 2014
Japanese

コリネ菌の細胞表層提示技術を用いたデンプンからの乳酸様ポリマーの生産
KADOYA KYOSUKE, SONG YUYANG, TOBITANI KOTA, MATSUMOTO KEN'ICHIRO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2013, 38, 27 Nov. 2013
Japanese

抗菌スペクトルを改変した抗菌ペプチド「アピデシン」特殊変異体の創成とその抗菌活性
YAMAZAKI KURATO, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2013, 37, 27 Nov. 2013
Japanese

組換えコリネ型細菌によるポリ乳酸様ポリマー生産の増強
TOBITANI KOTA, SASAMORI TETSUYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2013, 37, 27 Nov. 2013
Japanese

微生物重合系に基づく乳酸ポリマーからの拡張
TAGUCHI SEIICHI, MATSUMOTO, KEN'ICHIRO, 高分子学会予稿集(CD-ROM), 62, 2, ROMBUNNO.2X03, 28 Aug. 2013
Japanese

2‐ヒドロキシブタン酸ベースポリマーの微生物合成とその物性解析
MIYAKE MASAHIRO, TERAI SATSUKI, MATSUMOTO KEN'ICHIRO, KABE TAIZO, IWATA TADAHISA, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 65th, 238, 238, 25 Aug. 2013
日本生物工学会, Japanese

非可食性植物バイオマスからのP(3HB)の微生物生産
OI TOSHIHIKO, SASAKI SHOHEI, NDUKO JOHN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 65th, 237, 237, 25 Aug. 2013
日本生物工学会, Japanese

糖を利用した組換え大腸菌内でのグリコール酸ベースポリマーの生産
SHIBA TETSUFUMI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 65th, 237, 237, 25 Aug. 2013
日本生物工学会, Japanese

デンプンを炭素源に用いたポリ乳酸様ポリマーの合成
KADOYA RYOSUKE, SONG YUYANG, TOBITANI KOTA, MATSUMOTO KEN'ICHIRO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 65th, 238, 238, 25 Aug. 2013
日本生物工学会, Japanese

植物バイオマスを用いた組換え大腸菌による共重合ポリエステルの生産
SASAKI SHOHEI, OI TOSHIHIKO, NDUKO JOHN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 65th, 237, 237, 25 Aug. 2013
日本生物工学会, Japanese

ポリヒドロキシアルカン酸重合酵素の機能改変によるポリマー構造制御
MATSUMOTO KEN'ICHIRO, OCHI ANNA, OBA TAKASHI, TAKAYA MASAHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 65th, 237, 237, 25 Aug. 2013
日本生物工学会, Japanese

不飽和モノマーを導入した2‐ヒドロキシブタン酸ベースポリマーの生合成とその応用
CHOI YOONGYOO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 65th, 238, 238, 25 Aug. 2013
日本生物工学会, Japanese

Metabolic Chemistry of Microbial Production of Lactic Acid and Lactate-based Polyesters
MATSUMOTO Ken'ichiro, NDUKO John Masani, TAGUCHI Seiichi, KAGAKU TO SEIBUTSU, 51, 7, 448, 456, 01 Jul. 2013
生物史上初めて乳酸が微生物細胞内でポリマーの形で合成された．自然界になかった"乳酸重合酵素"の開発がもたらしたブレークスルーである．これを契機に，伝統的な"乳酸発酵"から"乳酸ポリマー発酵"という新しい概念が誕生することになる．本稿では，「炭素モル濃度」という尺度を導入した「メタボリックケミストリー」の切り口から，両発酵の化学量論的考察を加えた．今後，メタボロミクス分野で議論するうえで一つの化学的指標を与えるであろう．, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Japanese

Ralstonia eutropha由来PHA重合酵素の改変による2‐ヒドロキシブタン酸重合能力の強化
OCHI ANNA, OBA TAKASHI, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2013, 4C12A05 (WEB ONLY), 05 Mar. 2013
Japanese

PhaB高活性体を用いた光学活性(R)‐3‐ヒドロキシブタン酸生産の向上
OI TOSHIHIKO, HONMA IORI, WATANABE TSUYOSHI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2013, 4C12A08 (WEB ONLY), 05 Mar. 2013
Japanese

PLAを蓄積した大腸菌の透過型電子顕微鏡観察
WAKABAYASHI AIKO, SATO MICHIO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, MAEDA MICHIHISA, 日本顕微鏡学会関東支部講演会予稿集, 37th, 41, 05 Mar. 2013
Japanese

微生物ポリエステル合成系酵素の機能改変と多元ポリ乳酸の創製
MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2013, 4SY06-5 (WEB ONLY), 05 Mar. 2013
Japanese

動力学的解析および立体構造に基づいた進化型アセトアセチル‐CoAレダクターゼの高活性化メカニズムの解明
WATANABE TSUYOSHI, MATSUMOTO KEN'ICHIRO, MOTOHASHI REN, IKEDA KOJI, TANAKA YOSHIKAZU, MIN YAO, TANAKA ISAO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2013, 4C12A09 (WEB ONLY), 05 Mar. 2013
Japanese

改変型重合酵素による2‐ヒドロキシブタン酸ベースポリマーの生合成と物性解析
AOKI SHUNSUKE, OBA TAKASHI, OCHI ANNA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2013, 4C12A10 (WEB ONLY), 05 Mar. 2013
Japanese

Corynebacterium glutamicumをプラットフォームとしたポリ乳酸様ポリマー合成系の効率化のための代謝工学および培養工学的アプローチ
TOBITANI KOTA, SONG YUYANG, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2013, 4C12A12 (WEB ONLY), 05 Mar. 2013
Japanese

バイオプラ最前線 微生物はどこまでのポリエステルを合成できるか
松本 謙一郎, 田口 精一, バイオプラジャーナル, 12, 4, 12, 17, 01 Feb. 2013
日本バイオプラスチック協会 ; 2007-, Japanese, Introduction research institution

Microbial plastic factory: Synthesis and properties of the new lactate-based biopolymers
John Masani Nduko, Ken'Ichiro Matsumoto, Seiichi Taguchi, ACS Symposium Series, 1144, 175, 197, 2013, [Peer-reviewed]
American Chemical Society, English

Development of Integrated Process for Microbial Bioplastic Production from Plant Biomass
大井俊彦, 大井俊彦, 松本謙一郎, 門屋亨介, 門屋亨介, 田口精一, 田口精一, 高分子論文集, 70, 12, 675, 683, 2013
Society of Polymer Science, Japanese, Book review

可変領域への変異導入とその組み合わせによる抗菌ペプチド「アピデシン」の抗菌スペクトル改変
MIYAKE MASAHIRO, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2012, 25, 01 Nov. 2012
Japanese

セルロース系糖質バイオマスを原料とした高乳酸分率ポリマーの効率的生産
SASAKI KATSUHEI, NDUKO JOHN MASANI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2012, 26, 01 Nov. 2012
Japanese

進化工学的改変による高活性PhaBの速度論的解析とPHB生産性の向上
WATANABE TAKESHI, SO IKUYO, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2012, 27, 01 Nov. 2012
Japanese

Ralstonia eutropha由来ポリヒドロキシアルカン酸重合酵素の改変によるClass I乳酸重合酵素の創出
OCHI ANNA, OBA TAKASHI, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2012, 26, 01 Nov. 2012
Japanese

乳酸重合活性を有するClass Iポリヒドロキシアルカン酸(PHA)重合酵素の創出
OBA TAKASHI, OCHI ANNA, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 64th, 27, 27, 25 Sep. 2012
日本生物工学会, Japanese

CoA転移酵素を用いた大腸菌による3‐ヒドロキシブタン酸の生産
OI TOSHIHIKO, MATSUMOTO KEN'ICHIRO, OKEI TAKEHIRO, HONMA INORI, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 64th, 26, 26, 25 Sep. 2012
日本生物工学会, Japanese

2‐ヒドロキシブタン酸ベース新奇バイオプラスチックの微生物合成と物性解析
TERAI SATSUKI, ISHIYAMA AYAKO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 64th, 27, 27, 25 Sep. 2012
日本生物工学会, Japanese

糖質バイオマスから多様なポリエステルを生産するコリネ菌微生物工場の開発
SONG YUYANG, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 64th, 27, 27, 25 Sep. 2012
日本生物工学会, Japanese

Microbial plastic factory: Synthesis and properties of the new lactate-based and related biopolymers
John M. Nduko, Ken'Ichiro Matsumoto, Yuyang Song, Seiichi Taguchi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 244, Aug. 2012, [Peer-reviewed]
English, Summary international conference

Biosynthesis and properties of advanced biopolymers containing lactate by metabolically engineered bacteria
John M. Nduko, Ken'ichiro Matsumoto, Seiichi Taguchi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 244, Aug. 2012, [Peer-reviewed]
English, Summary international conference

Corynebacterium glutamicumの細胞表層提示技術を用いたデンプンからのポリヒドロキシブタン酸(PHB)の直接生産
TSUDA HARUNA, SONG YUYANG, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, KONDO AKIHIKO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2012, 2C01P05 (WEB ONLY), 05 Mar. 2012
Japanese

組換え大腸菌による(R)‐3‐ヒドロキシブタン酸の生産
OI TOSHIHIKO, MATSUMOTO KEN'ICHIRO, OKEI TAKEHIRO, HONMA INORI, WATANABE TSUYOSHI, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2012, 2C01P07 (WEB ONLY), 05 Mar. 2012
Japanese

アセトアセチルCoAレダクターゼ高活性変異体の解析とそれを用いたCorynebacterium glutamicumによるPHB生産の増強
WATANABE TSUYOSHI, SONG YUYANG, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2012, 2C01P06 (WEB ONLY), 05 Mar. 2012
Japanese

進化工学による新規乳酸重合酵素の探索
OCHI ANNA, OBA TAKASHI, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2012, 2C01P08 (WEB ONLY), 05 Mar. 2012
Japanese

抗菌ペプチド「アピデシン」の可変領域への変異導入とその抗菌活性への影響
MIYAKE MASAHIRO, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集(Web), 2012, 4A07A01 (WEB ONLY), 05 Mar. 2012
Japanese

Direct conversion of glucose into lactate-based polyesters using engineered Corynebacterium glutamicum as a whole cell catalyst
Yuyang Song, Ken'ichiro Matsumoto, Miwa Yamada, Aoi Gohda, Christopher J. Brigham, Anthony J. Sinskey, Seiichi Taguchi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 243, Mar. 2012, [Peer-reviewed]
English, Summary international conference

Bioconversion of the hemicellulosic sugar, xylose, into lactate-based polyesters using recombinant Escherichia coli
John M. Nduko, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 243, Mar. 2012, [Peer-reviewed]
English, Summary international conference

Flavin-binding of azoreductase: Direct evidences for dual-binding property of apo-azoreductase with FMN and FAD
Toshihiko Ooi, Daiki Ogata, Ken'ichiro Matsumoto, Gen Nakamura, Jian Yu, Min Yao, Masaya Kitamura, Seiichi Taguchi, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 74, 3-4, 204, 208, Feb. 2012
English

組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成と物性解析
斯波哲史, 石山絢子, 松本謙一郎, 田口精一, 日本農芸化学会大会講演要旨集(Web), 2012, 2012

Biosynthesis of novel lactate(LA)-based polyester incorporating unsaturated monomer unit by using recombinant Escherichia coli.
SUN Jian, SHOZUI Fumi, MATSUMOTO Ken’ichiro, TAGUCHI Seiichi, 日本農芸化学会大会講演要旨集(Web), 2012, 2012

Microbial Plastic Factory: metabolic-enzyme engineering
NDUKO John Masani, MATSUMOTO Ken’ichiro, TAGUCHI Seiichi, 日本農芸化学会大会講演要旨集(Web), 2012, 2012

バイオリファイナリーの今,そして未来 Microbial Plastic Factory-ポリ乳酸から多元ポリ乳酸の時代へ-
松本謙一郎, 田口精一, 生物工学会誌, 90, 7, 411, 414, 2012
公益社団法人日本生物工学会, Japanese

Lucky Enzyme
MATSUMOTO Ken'ichiro, 高分子, 60, 9, 683, 683, 01 Sep. 2011
Japanese

真菌に対する抗菌ペプチドThanatin誘導体の作用評価
OSHIMA YU, SAITO JUNKI, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 63rd, 149, 149, 25 Aug. 2011
日本生物工学会, Japanese

進化工学的手法による高乳酸分率のポリ(乳酸‐co‐3‐ヒドロキシブタン酸)が合成可能な乳酸重合酵素の探索
TERAI SATSUKI, SHOZUI FUMI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 63rd, 232, 232, 25 Aug. 2011
日本生物工学会, Japanese

組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成
ISHIYAMA AYAKO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 63rd, 232, 232, 25 Aug. 2011
日本生物工学会, Japanese

進化型PhaBによる組換えタバコにおけるPHB生産性の増強
OBA TAKASHI, EGUCHI AYA, MORIMOTO KENJIRO, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 63rd, 232, 232, 25 Aug. 2011
日本生物工学会, Japanese

進化工学的改変により得られたアセトアセチル‐CoAレダクターゼ高活性変異体の解析
WATANABE TSUYOSHI, HAYASAKA SATOSHI, IKEDA KOJI, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 63rd, 231, 231, 25 Aug. 2011
日本生物工学会, Japanese

抗菌ペプチドThanatin誘導体の酵母様真菌に対する抗菌活性
OSHIMA YU, SAITO JUNKI, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2011, 22, 05 Mar. 2011
Japanese

PHA生合成系におけるモノマー供給酵素アセトアセチル‐CoAレダクターゼの高活性変異体の酵素反応解析
WATANABE TAKESHI, HAYASAKA SATOSHI, IKEDA KOJI, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2011, 4, 05 Mar. 2011
Japanese

乳酸ベース共重合体P(lactate‐co‐3‐hydroxybutyrate‐co‐3‐hydroxyvalerate)の生合成および物性評価
SON KEN, MOTOHASHI REN, SHOZUI AYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2011, 4, 05 Mar. 2011
Japanese

抗菌ペプチド「アピデシン」のN末端領域分子デザインによる高活性変異体の作製と抗菌スペクトル評価
WAKABAYASHI MASAAKI, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2011, 21, 05 Mar. 2011
Japanese

高活性化PhaBの導入による組換えタバコでのPHB生産性の向上
OBA TAKASHI, EGUCHI AYA, MORIMOTO KENJIRO, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2011, 4, 05 Mar. 2011
Japanese

高乳酸分率のポリ(乳酸‐co‐3‐ヒドロキシブタン酸)[P(LA‐co‐3HB)]が合成可能な乳酸重合酵素の探索
TERAI SATSUKI, SHOZUI AYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2011, 5, 05 Mar. 2011
Japanese

グルコースを炭素源とした乳酸ベースポリマーの微生物生産
AIDA AOI, SO IKUYO, MATSUMOTO KEN'ICHIRO, YAMADA MIWA, TAGUCHI SEIICHI, 高分子学会北海道支部研究発表会講演要旨集, 45th, 77, 01 Feb. 2011
Japanese

Poly(lactate‐co‐3‐hydroxybutyrate‐co‐3‐hydroxyvalerate)の生合成および物性評価
WATANABE TAKESHI, MOTOHASHI TADASHI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 高分子学会北海道支部研究発表会講演要旨集, 45th, 26, 01 Feb. 2011
Japanese

Efficient xylose utilization in microbial production of lactate-based polymer
MASANI Nduko John, MATSUMOTO Ken’ichiro, TAGUCHI Seiichi, 高分子学会北海道支部研究発表会講演要旨集, 45th, 2011

2Ka02 Biosynthesis of poly(lactate-co-3-hydroxybutyrate) in endotoxin-free Corynebacterium glutamicum expressing lactatepolymerizing enzyme :
Song Yuyang, Gohda Aoi, Matsumoto Ken'ichiro, Yamada Miwa, Taguchi Seiichi, 日本生物工学会大会講演要旨集, 63, 187, 187, 2011
日本生物工学会, English

2Ka03 Production of lactate-based polyesters from xylose in Escherichia coli :
Nduko John Masani, Matsumoto Ken'ichiro, Ooi Toshihiko, Taguchi Seiichi, 日本生物工学会大会講演要旨集, 63, 187, 187, 2011
日本生物工学会, English

2Bp13 Biosynthesis of flexible lactate-based copolymers incorporating 3-hydroxyvalerate unit in recombinant Escherichia coli by feeding of propionate :
Sun Jian, Motohashi Ren, Shozui Fumi, Matsumoto Ken'ichiro, Taguchi Seiichi, 日本生物工学会大会講演要旨集, 63, 123, 123, 2011
日本生物工学会, English

組換え大腸菌を利用した(R)‐3‐ヒドロキシ酪酸の生産
OKEI GAKUYO, HONMA INORI, MATSUMOTO KEN'ICHIRO, WATANABE TAKESHI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2011, 8, 2011
Japanese

乳酸およびグリコール酸ベース新奇バイオプラスチックの微生物生産
SHIBA TETSUSHI, ISHIYAMA AYAKO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2011, 9, 2011
Japanese

高乳酸分率ポリ(乳酸‐co‐3‐ヒドロキシブタン酸)合成のための乳酸重合酵素の進化工学的改変
SAKAI KOHEI, TERAI SATSUKI, SHOZUI AYA, OCHI ANNA, MATSUMOTO, KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2011, 8, 2011
Japanese

アミノ酸残基置換による抗菌ペプチド「アピデシン」の抗菌スペクトルの改変
IWAMURA YUTA, YAMAGATA KYOKO, WAKABAYASHI MASAAKI, MATSUMOTO, KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会北海道支部講演会講演要旨, 2011, 1, 2011
Japanese

One-pot Production of Lactate-Based Polyesters Using Engineered Microbes Expressing Lactate-Polymerizing Enzyme
Ken'ichiro Matsumoto, Seiichi Taguchi, KOBUNSHI RONBUNSHU, 68, 5, 271, 280, 2011
Japanese

Production of Polyhydroxyalkanoate Copolymers in Transgenic Plants Expressing Engineered Enzymes
松本謙一郎, 高分子論文集, 68, 8, 562, 569, 2011
Japanese

ポリヒドロキシアルカン酸生合成系のモノマー供給酵素acetoacetyl‐CoA reductaseの進化工学的改変
HAYASAKA SATOSHI, MOTOHASHI REN, SHOZUI FUMI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 62nd, 165, 165, 25 Sep. 2010
日本生物工学会, Japanese

組換え大腸菌を用いたポリ(乳酸‐co‐3‐ヒドロキシブタン酸‐co‐3‐ヒドロキシヘキサン酸)の生産
SHOZUI FUMI, MOTOHASHI REN, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 62nd, 166, 166, 25 Sep. 2010
日本生物工学会, Japanese

N末分子デザインによる抗菌ペプチド「アピデシン」の高活性体創出
YAMAGATA KYOKO, HASHIMOTO SHIGEKI, ORIKASA YOSHITAKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 62nd, 178, 178, 25 Sep. 2010
日本生物工学会, Japanese

抗菌ペプチドThanatinのシステイン部位に導入されたアルキル基の側鎖長が抗菌活性に及ぼす影響
SAITO JUNKI, ORIKASA YOSHITAKE, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 62nd, 178, 178, 25 Sep. 2010
日本生物工学会, Japanese

Pseudomonas sp.61‐3由来PHA重合酵素の新規高活性変異体の取得と機能解析
SAKAI KOHEI, SHOZUI FUMI, MATSUMOTO KEN'ICHIRO, TAKASE KAZUMA, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 62nd, 165, 165, 25 Sep. 2010
日本生物工学会, Japanese

新奇かつデザイナブルなバイオポリエステル合成のための重合酵素の機能改変
MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本蛋白質科学会年会プログラム・要旨集, 10th, 38, 15 May 2010
Japanese

領域指定変異による抗菌ペプチド”Apidaecin”変異体の作製とその抗菌活性評価
岩村雄太, 山形享子, 折笠善丈, 橋本茂樹, 松本謙一郎, 大井俊彦, 田口精一, 日本農芸化学会大会講演要旨集, 2010, 249, 05 Mar. 2010
Japanese

ジャーファーメンターを用いた乳酸ベースポリマーの微生物発酵生産
URAMOTO SHU, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, ABE HIDEKI, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2010, 292, 05 Mar. 2010
Japanese

乳酸重合酵素への新規変異導入による乳酸コポリマーの分率制御
YAMADA MIWA, SHIMIZU KOTARO, URAMOTO SHU, NAKAI TAKANORI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2010, 292, 05 Mar. 2010
Japanese

ルテニウム触媒によるセルロース糖化物を用いたポリヒドロキシブタン酸(PHB)の微生物生産
IKEDA KOJI, MATSUMOTO KEN'ICHIRO, KOMANOYA TASUKU, KOBAYASHI HIROKAZU, OI TOSHIHIKO, FUKUOKA ATSUSHI, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2010, 292, 05 Mar. 2010
Japanese

3‐ヒドロキシブタン酸ベースバイオポリマーの組成制御を目指したモノマー供給酵素の進化工学的改変
SHOZUI AYA, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本農芸化学会大会講演要旨集, 2010, 292, 05 Mar. 2010
Japanese

立体選択的乳酸ベースポリマーのバイオ合成と構造解析
YAMADA MIWA, NAKAI TAKANORI, SHIMIZU KOTARO, URAMOTO SHU, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 高分子学会北海道支部研究発表会講演要旨集, 44th, 48, 26 Jan. 2010
Japanese

3‐ヒドロキシ吉草酸ユニットを含む新規乳酸ベース3元共重合体の微生物合成
MASAMIZU FUMI, NAKAI TAKANORI, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 高分子学会北海道支部研究発表会講演要旨集, 44th, 49, 26 Jan. 2010
Japanese

Improving the monomer-supplying function of FabH for polyhydroxyalkanoate production through site-specific mutagenesis
MUELLER Alexander P, MATSUMOTO Ken’ichiro, TAGUCHI Seiichi, NOMURA Christopher T, 日本生物工学会シンポジウム講演要旨集, 2010, 2010

担持ルテニウム触媒によるセルロース糖化反応を利用したポリヒドロキシブタン酸(PHB)の微生物生産
MATSUMOTO KEN'ICHIRO, IKEDA KOJI, KOMANOYA TASUKU, KOBAYASHI HIROKAZU, FUKUOKA ATSUSHI, TAGUCHI SEIICHI, 日本生物工学会シンポジウム講演要旨集, 2010, 29, 2010
Japanese

乳酸ポリマー生産用微生物工場の開発
TAGUCHI SEIICHI, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, NAKAI TAKANORI, SHIMIZU KOTARO, 高分子学会予稿集(CD-ROM), 58, 2 Disk1, ROMBUNNO.1U-18, 01 Sep. 2009
Japanese

抗菌ペプチドApidaecinの高活性化のための一戦略:細胞内導入量の向上
YAMAGATA KYOKO, ICHINOHE KENTA, ORIKASA YOSHITAKE, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 61st, 36, 36, 25 Aug. 2009
日本生物工学会, Japanese

Bacillus sp.B29由来アゾ還元酵素基質複合体の結晶構造解析
OGATA DAIKI, TAGUCHI SEIICHI, MATSUMOTO KEN'ICHIRO, FUJIWARA TAKAAKI, TANAKA ISAO, YAO MIN, OI TOSHIHIKO, 日本生物工学会大会講演要旨集, 61st, 62, 62, 25 Aug. 2009
日本生物工学会, Japanese

抗菌ペプチドThanatinおよびその高活性化学修飾体の細菌に対する作用
ORIKASA YOSHITAKE, SAITO JUNKI, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 61st, 36, 36, 25 Aug. 2009
日本生物工学会, Japanese

組換え大腸菌を用いた新規乳酸ベース3元共重合体の生産
SHOZUI FUMI, NAKAI TAKANORI, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, 日本生物工学会大会講演要旨集, 61st, 191, 191, 25 Aug. 2009
日本生物工学会, Japanese

乳酸ベースポリエステルの微生物発酵生産
山田美和, 松本謙一郎, 田島健二, 佐藤慶治, 棟方正信, 幸田勝典, 嶋村隆, 神戸浩美, 小畑充生, 田口精一, 日本農芸化学会大会講演要旨集, 2009, 331, 05 Mar. 2009
Japanese

乳酸ベースポリエステルの生合成における培養条件の検討
清水康多郎, 浦本周, 山田美和, 松本謙一郎, 田口精一, 日本農芸化学会大会講演要旨集, 2009, 332, 05 Mar. 2009
Japanese

嫌気条件下における乳酸ベースポリエステルの微生物発酵生産
中井孝憲, 山田美和, 松本謙一郎, 田口精一, 日本農芸化学会大会講演要旨集, 2009, 331, 05 Mar. 2009
Japanese

組換え微生物を用いた乳酸ベースポリマーの発酵生産
山田美和, 中井孝憲, 清水康多郎, 浦本周, 松本謙一郎, 田口精一, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 57, 03 Feb. 2009
Japanese

酵素の進化工学を応用した新規バイオポリマー生産系の開発
松本謙一郎, 山田美和, 中井孝憲, 清水康多郎, 浦本周, 田口精一, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 14, 03 Feb. 2009
Japanese

PHB生合成系酵素群の発現効率化の組換え体タバコにおけるPHB生産性への影響
森本健二郎, 合田葵, 松本謙一郎, 島田浩章, 田口精一, 日本農芸化学会大会講演要旨集, 2009, 2009

Microbial Production of Lactate-based Polyesters
MATSUMOTO Ken’ichiro, YAMADA Miwa, NAKAI Takanori, SHIMIZU Koutaro, TAGUCHI Seiichi, 高分子学会予稿集(CD-ROM), 58, 1 Disk1, 2009

嫌気的条件下における乳酸ベースポリマーの微生物発酵生産
浦本周, 中井孝憲, 山田美和, 松本謙一郎, 田口精一, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2009, 15, 2009
Japanese

Pseudomonas sp.61‐3由来PHA重合酵素変異体による多様な分子量をもつPHAの合成
正瑞文, 清水康多郎, 佐々木雄大, 折笠善丈, 松本謙一郎, 大井俊彦, 田口精一, 日本農芸化学会大会講演要旨集, 2008, 42, 05 Mar. 2008
Japanese

Pseudomonas sp.61‐3由来PHA重合酵素変異体による組換え大腸菌体内でのPHA共重合体の蓄積率とポリマー分子量の相関
正瑞文, 清水康多郎, 佐々木雄大, 折笠善丈, 松本謙一郎, 田口精一, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 39, 29 Jan. 2008
Japanese

1Cp03 Production of poly(3-hydroxybutyrate) through a new biosynthetic pathway utilizing malonyl-CoA as an intermediate :
Leong Chean Ring, Matsumoto Ken'ichiro, Ooi Toshihiko, Kuzuyama Tomohisa, Taguchi Seiichi, 日本生物工学会大会講演要旨集, 20, 93, 93, 2008
日本生物工学会, English

1Fp08 Crystallization and X-ray analysis of an azoreductase from Bacillus sp. B29
OGATA Daiki, TAGUCHI Seiichi, MATSUMOTO Ken'ichiro, YAO Min, OOI Toshihiko, 日本生物工学会大会講演要旨集, 20, 154, 154, 2008
日本生物工学会, Japanese

1S1p03 Bioplastic Production using Artificially Evolved Enzymes : Bacteria to Plants
MATSUMOTO Ken'ichiro, SHIMADA Hiroaki, TAGUCH Seiichi, 日本生物工学会大会講演要旨集, 20, 4, 4, 2008
日本生物工学会, Japanese

食品包装の動向 酵素進化工学を応用したバイオポリマー生産システムの開発
松本謙一郎, 食品の包装, 39, 2, 2008

Microbial Factory for the Production of Bioplastics
山田美和, 松本謙一郎, 田口精一, 繊維学会誌, 64, 11, P365, P370, 2008
Japanese

遺伝子組換え植物による生分解性プラスチック生産法の開発。　　　　　　　　　　　　　　　
松本謙一郎, 島田浩章, 田口精一, エコマテリアル研究会, 5, Jul. 2007
Japanese

Production of polyhydroxyalkanoate copolymer in plastids of Arabidopsis thaliana　　　　　　　　　　　　　　　
Ken'ichiro Matsumoto, Satoshi Arai, Christopher Nomura, Takaaki Murata, Rina Nagao, Hiroaki Shimada, ICPMB2007, International Congress on Plant Mitochondrial Biology, 不明, Jun. 2007
English

Pseudomonas sp.61-3由来PHA重合酵素の推定基質ポケット近傍のアミノ酸残基群の配列と機能の相関
佐々木雄大, 正瑞文, 松本謙一郎, 田口精一, 日本農芸化学会大会講演要旨集, 2007, 2007

2E17-2 Enhancement of polyhydroxybutyrate (PHB) production by Corynebacterium glutamicum using evolutionary engineering
JO Sung-Jin, LEONG Chean Ring, MATSUMOTO Ken'ichiro, OOI Toshihiko, TAGUCHI Seiichi, 日本生物工学会大会講演要旨集, 19, 125, 125, 2007
日本生物工学会, Japanese

2E17-1 Correlation between molecular weight of PHA and amino acid residues in hypothetical substrate binding pocket of Pseudomonas sp. 61-3 PHA synthase
SHOZUI Fumi, SHIMIZU Kotaro, SASAKI Takahiro, MATSUMOTO Ken'ichiro, TAGUCHI Seiichi, 日本生物工学会大会講演要旨集, 19, 124, 124, 2007
日本生物工学会, Japanese

PHA synthase engineering toward super biocatalysts for custom-made biopolymers
MATSUMOTO Kenichiro, TAGUCHI Seiichi, Bioscience & industry, 65, 7, 334, 339, 2007
バイオインダストリー協会, Japanese

Production of polyhydroxybutylate in cultured transgenic-rice cells　　　　　　　　　　　　　　　
Hiroaki Shimada, Atsuo Kawamura, Ken'ichiro Matsumoto, 8th International Congress of Plant Moleculat Biology, 不明, Aug. 2006
English

Production of polyhydrpxyalkanoate (PHA) copolymer in transgenic plants and development of plant tissue-specific PHA production system　　　　　　　　　　　　　　　
Ken'ichiro Matsumoto, Takaaki Murata, Christopher T. Nomura, Atuso Kawamura, Miho Yoshida, Satoshi Arai, Rina Nagao, Yuko Arai, Seiichi Taguchi, Hiroaki Shimada, International Symposium on Biological polymer 2006, 不明, Aug. 2006
English

Production of polyhydroxyalkanoate (PHA) in the transgenic plants　　　　　　　　　　　　　　　
Ken'ichiro Matsumoto, Atsuo Kawamura, Miho Yoshida, Satoshi Arai, Takaaki Murata, Rina Nagao, Hioraki Shimada, 20th IUBMB & 11th FAOBMB, 不明, Jun. 2006
English

遺伝子組換えイネによるポリヒドロキシアルカン酸の合成　　　　　　　　　　　　　　　
川村温夫, 松本謙一郎, 島田浩章, 育種学研究, 8, 別冊1, 不明, Mar. 2006
Japanese

Creation of Eco‐friendly Plastics by Biotechnological Application: From Microbial Production to Production in Plants
山田美和, 松本謙一郎, 田口精一, オレオサイエンス, 5, 11, 523, 532, 01 Nov. 2005
Japanese

進化工学的手法により改変されたPseudomonas sp.61‐3由来Polyhydroxyalkanoate(PHA)合成酵素(PhaC1)の精製と解析
佐藤康治, 勝田直介, 田島健次, 中沢浩二, 松本謙一郎, 高瀬和真, 田口精一, 土肥義治, 陳方正信, 日本農芸化学会大会講演要旨集, 2005, 237, 05 Mar. 2005
Japanese

Pseudomonas sp.61-3のポリヒドロキシアルカン酸生合成遺伝子の解析
磯田美子, 川口桃子, 宮村ゆかり, 松本謙一郎, 土肥義治, 松崎弘美, 日本農芸化学会大会講演要旨集, 2005, 2005

3-ketoacyl-ACP synthase III(FabH)遺伝子を用いた遺伝子組換えシロイヌナズナによるポリヒドロキシアルカン酸共重合体の合成
松本謙一郎, 松本謙一郎, 村田隆明, 長尾里奈, 野村クリストファー, 新井祐子, 高瀬和真, 仲下英雄, 田口精一, 土肥義治, 島田浩章, 日本分子生物学会年会講演要旨集, 28th, 2005

バイオミディア 植物が作るプラスチック
松本謙一郎, 生物工学会誌, 83, 9, 2005

人工進化型polyhydroxyalkanoate(PHA) synthaseのkinetic analysis
松本謙一郎, 佐藤康治, 田島健次, 棟方正信, 高瀬和真, 土肥義治, 田口精一, 高分子学会予稿集(CD−ROM), 53, 2 Disk1, 3PB178, 01 Sep. 2004
Japanese

2B11-5 Composition control of PHA copolymer by molecular evolutionary engineering of Pseudomonas sp. 61-3 PHA synthase
AOKI Emi, TAKASE Kazuma, MATSUMOTO Ken'ichiro, DOI Yoshiharu, TAGUCHI Seiichi, 日本生物工学会大会講演要旨集, 16, 122, 122, 2004
日本生物工学会, Japanese

3C10-5 Enhancement of polyhydroxyalkanoates (PHA) production in transgenic Arabidopsis thaliana by the highly active mutated PHA synthase generated by in vitro evolution
MATSUMOTO Ken'ichiro, ARAI Yuko, NAGAO Rina, MURATA Takaaki, TAKASE Kazuma, TAGUCHI Seiichi, SHIMADA Hiroaki, DOI Yoshiharu, KICHISE Tomoyasu, NAKASHITA Hideo, 日本生物工学会大会講演要旨集, 16, 145, 145, 2004
日本生物工学会, Japanese

In Vitro Analysis of Altered Substrate Specificity of Polyhydroxyal-kanoate (PHA) synthases which were generated by Means of PCR-mediated mutagenesis of PHA synthase gene
Matsumoto Kenichiro, Takase Kazuma, Taguchi Seiichi, Doi Yoshiharu, 日本生物工学会大会講演要旨集, 15, 67, 67, 2003
日本生物工学会, Japanese

Characterization of PHAs and their granule-associated proteins in Pseudomonas sp. 61-3
Isoda Yoshiko, Matsumoto Kenichiro, Doi Yoshiharu, Matsusaki Hiromi, 日本生物工学会大会講演要旨集, 15, 207, 207, 2003
日本生物工学会, Japanese

Biosynthesis of Biodegradable Polyester: Current Research and Future Direction
松本謙一郎, 土肥義治, 有機合成化学協会誌, 61, 5, 489, 495, 2003
Society of Synthetic Organic Chemistry, Japanese

Pseudomonas sp.61-3のポリヒドロキシアルカン酸結合タンパク質の解析
松本謙一郎, 松崎弘美, 田口一徳, 関実, 土肥義治, 高分子学会予稿集, 51, 14, 2002

1031 Analysis of PHA synthesis related genes in Pseudomonas sp. 61-3 and recognition of PHA composition by PHA inclusion associated proteins
Fujino Kanako, Matsumoto Ken'ichiro, Doi Yoshiharu, Matsusaki Hiromi, 日本生物工学会大会講演要旨集, 14, 194, 194, 2002
日本生物工学会, Japanese

527 Characterization of PHA inclusions and their associated proteins in Pseudomonas sp. 61-3
Matsumoto Ken'ichiro, Matsusaki Hiromi, Taguchi Kazunori, Doi Yoshiharu, 日本生物工学会大会講演要旨集, 14, 79, 79, 2002
日本生物工学会, Japanese

de novo脂肪酸合成系を経由するポリエステル生合成経路の解析と応用
松本謙一郎, 関実, 松崎弘美, 田口一徳, 田口精一, 土肥義治, 高分子学会予稿集, 50, 5, 2001

生分解性プラスチック生合成のための脂肪酸合成系酵素遺伝子の解析
松本謙一郎, 関実, 松崎弘美, 田口精一, 土肥義治, 高分子学会予稿集, 49, 5, 2000

Real-time NMR Analysis of Polyhydroxyalkanoate Synthase Reaction That Synthesizes Block Copolymer of Glycolate and 3-Hydroxybutyrate
Yanagawa Kengo, Kajikawa Ayaka, Sakakibara Sayaka, Kumeta Hiroyuki, Tomita Hiroya, Matsumoto Ken’ichiro
Japan Sciety for Environmental Biotechnology, 2023, Japanese

Poly(lactic acid): Synthesis, Structures, Properties, Processing, Applications, and End of Life, 2nd Edition　　　　　　　　　　　　　　　
Tadahisa Iwata, Hideki Abe, Yoshihiro Kikkawa, Ken’ichiro Matsumoto, Part of Chapter 22
Wiley, Jun. 2022, 9781119767442, [Contributor]

Expanding artificial polyhydroxyalkanoates: cross-point of PLA biosynthesis and block copolymerization　　　　　　　　　　　　　　　
Ken'ichiro Matsumoto
The international symposium on biopolymers, 21 Oct. 2024, English, Invited oral presentation
20 Oct. 2024 - 23 Oct. 2024, [Invited]

Mechanistic analysis of polyhydroxyalkanoate block copolymerization　　　　　　　　　　　　　　　
柳川謙吾, 冨田宏矢, 蜂須賀真一, 菊川寛史, 松本謙一郎
日本農芸化学会大会講演要旨集(Web), 2024
2024 - 2024

Analysis of energy metabolism in the polyhydroxyalkanoate production process by microscopic observation method　　　　　　　　　　　　　　　
富士航至, 梶川彩香, 蜂須賀真一, 冨田宏矢, 菊川寛史, 今村博臣, 松本謙一郎
日本農芸化学会大会講演要旨集(Web), 2024
2024 - 2024

Mechanistic analysis of the enzymatic synthesis of polyhydroxyalkanoates with block sequence　　　　　　　　　　　　　　　
柳川謙吾, 冨田宏矢, 蜂須賀真一, 菊川寛史, 松本謙一郎
日本生物工学会大会講演要旨集, 2024
2024 - 2024

長主鎖モノマーを含む非天然型ポリヒドロキシアルカン酸の律速段階解析に基づく代謝工学的改変　　　　　　　　　　　　　　　
田近誠也, 蜂須賀真一, 菊川寛史, 松本謙一郎
日本栄養・食糧学会北海道支部大会講演要旨集, 05 Aug. 2023, Japanese, Poster presentation
05 Aug. 2023 - 05 Aug. 2023

ブロック配列型微生物ポリエステルの酵素分解系の構築　　　　　　　　　　　　　　　
伊関叶互, 蜂須賀真一, 菊川寛史, 松本謙一郎
日本栄養・食糧学会北海道支部大会講演要旨集, 05 Aug. 2023, Japanese, Poster presentation
05 Aug. 2023 - 05 Aug. 2023

ポリ乳酸セグメントを含む新規ブロック共重合体の微生物合成と物性制御　　　　　　　　　　　　　　　
今井昂渡, 蜂須賀真一, 菊川寛史, 冨田宏矢, 松本謙一郎
日本栄養・食糧学会北海道支部大会講演要旨集, 05 Aug. 2023, Japanese, Poster presentation
05 Aug. 2023 - 05 Aug. 2023

ポリヒドロキシアルカン酸生合成過程におけるエネルギー代謝動態観察　　　　　　　　　　　　　　　
富士航至, 梶川彩香, 冨田宏矢, 蜂須賀真一, 菊川寛史, 今村博臣, 松本謙一郎
日本栄養・食糧学会北海道支部大会講演要旨集, 05 Aug. 2023, Japanese, Poster presentation
05 Aug. 2023 - 05 Aug. 2023

Versatile aliphatic polyester biosynthesis system for producing random and block copolymers using engineered enzymes　　　　　　　　　　　　　　　
Ken'ichiro Matsumoto
ETH-Hokudai Joint symposium, 08 Mar. 2023, English, Nominated symposium
[Invited]

2-ヒドロキシブタン酸ベースポリマーの微生物分解性の解析　　　　　　　　　　　　　　　
井口綾実, 蜂須賀真一, 菊川寛史, 冨田宏矢, 大井俊彦, 松本謙一郎
日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2023
2023 - 2023

中鎖ヒドロキシアルカン酸CoA化酵素AlkKの基質特異性のin vivo/in vitro解析　　　　　　　　　　　　　　　
佐々木守, 佐藤圭悟, 柳川謙吾, 山本一輝, 蜂須賀真一, 菊川寛史, 冨田宏矢, 松本謙一郎
日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2023
2023 - 2023

非天然長主鎖モノマーを含むポリヒドロキシアルカン酸生合成のための重合酵素PhaC_ARの進化工学的改変　　　　　　　　　　　　　　　
穂積侑伽, 蜂須賀真一, 菊川寛史, 冨田宏矢, 松本謙一郎
日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2023
2023 - 2023

ポリヒドロキシアルカン酸合成系を利用したスクリーニングによるRuBisCOの高活性多重変異体の獲得　　　　　　　　　　　　　　　
芥川柚月, 渡邉秀平, 藤原悠暉, 蜂須賀真一, 菊川寛史, 蘆田弘樹, 松本謙一郎
日本栄養・食糧学会北海道支部大会講演要旨集, 2023
2023 - 2023

粘弾性PHAブロック共重合体の 合成と生分解　　　　　　　　　　　　　　　
松本謙一郎
生物工学会2022年年次大会シンポジウム, 18 Oct. 2022, Japanese, Invited oral presentation
17 Oct. 2022 - 20 Oct. 2022, [Invited]

微生物の中にプラスチック工場を作る　　　　　　　　　　　　　　　
北鍾総会, 08 Oct. 2022, Japanese, Public discourse
[Invited]

Biosynthesis and properties of sequence-regulated artificial polyhydroxyalkanoates　　　　　　　　　　　　　　　
Ken'ichiro Matsumoto
International Symposium on Biological Polymers, 14 Sep. 2022, English, Keynote oral presentation
[Invited]

進化分子工学による新規バイオ プラスチックの微生物合成　　　　　　　　　　　　　　　
松本謙一郎
トランスフォーマティブ化学生命融合研究大学院プログラム, 27 Apr. 2022, Japanese, Public discourse
[Invited]

Biosynthesis of polyhydroxyalkanoate block copolymer by engineered Ralstonia eutropha　　　　　　　　　　　　　　　
石原静流, 折田和泉, 松本謙一郎, 福居俊昭
日本農芸化学会大会講演要旨集(Web), 2022
2022 - 2022

Engineering of Ralstonia eutropha for biosynthesis of sequence-regulated copolyesters potentially useful as novel biodegradable plastics　　　　　　　　　　　　　　　
石原静流, 折田和泉, 松本謙一郎, 福居俊昭
日本生物工学会大会講演要旨集, 2022
2022 - 2022

Biosynthesis of elastic and biodegradable polyhydroxyalkanoate block copolymers　　　　　　　　　　　　　　　
松本謙一郎
日本生物工学会大会講演要旨集, 2022
2022 - 2022

改変型重合酵素を用いたグリコール酸ベースポリマー合成のin vitro解析　　　　　　　　　　　　　　　
山本一輝, 柳川謙吾, 冨田宏矢, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

改変型重合酵素PhaCARによる軟質性グリコール酸ポリマーの生合成　　　　　　　　　　　　　　　
田中聖也, 佐藤圭悟, 冨田宏矢, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

中鎖モノマー認識に向けたポリヒドロキシアルカン酸合成酵素の基質特異性の改変　　　　　　　　　　　　　　　
向井太一, HIEN Phan Thi, 冨田宏矢, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

(R)-2-ヒドロキシブタン酸ベースポリマー分解菌の探索　　　　　　　　　　　　　　　
井口綾実, 冨田宏矢, 大井俊彦, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

組み換え大腸菌を利用したポリヒドロキシアルカン酸生合成系スクリーニングによる光合成細菌由来RuBisCO高機能変異体の獲得　　　　　　　　　　　　　　　
渡邉秀平, 藤原悠輝, 永田暁洋, 工藤悠希, 松本直己, 齋藤樹里, 喜多幸, 冨田宏矢, 蘆田弘樹, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

配列制御型PHA酸合成酵素の基質による配列制御性の解析　　　　　　　　　　　　　　　
田近誠也, 佐藤圭悟, 冨田宏矢, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

配列制御型ポリヒドロキシアルカン酸合成酵素による新規ブロック共重合体の生合成および物性解析　　　　　　　　　　　　　　　
川上智也, 冨田宏矢, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

機能改変型ポリヒドロキシアルカン酸合成酵素のモノマー配列制御の特性解析　　　　　　　　　　　　　　　
富士航至, HIEN Phan Thi, 細江有美, MUREEN Guex, 友居昌慶, 冨田宏矢, MANFRED Zinn, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

High-throughput screening of highly active RuBisCO mutants using polyhydroxyalkanoate biosynthetic system　　　　　　　　　　　　　　　
渡邉秀平, 藤原悠暉, 永田暁洋, 工藤悠希, 松本直己, 齋藤樹里, 喜多幸, 冨田宏矢, 蘆田弘樹, 松本謙一郎
日本生物工学会大会講演要旨集, 2022
2022 - 2022

配列制御型ポリエステル合成酵素を用いたエラストマー様共重合体の生合成　　　　　　　　　　　　　　　
今井昂渡, 影山友基, 冨田宏矢, 磯野拓也, 佐藤敏文, 松本謙一郎
日本農芸化学会東北支部大会プログラム・講演要旨集, 2022
2022 - 2022

Biosynthesis of sequence-regulated and flexible glycolate-containing polymer by medium-chain-length monomer introduction.　　　　　　　　　　　　　　　
田中聖也, 冨田宏矢, 佐藤圭悟, NOMURA Christopher, 松本謙一郎
日本農芸化学会大会講演要旨集(Web), 2022
2022 - 2022

モノマー配列が制御された生分解性ポリエステルの微生物合成　　　　　　　　　　　　　　　
松本謙一郎
日本高分子学会講演会ポリマーフロンティア２１, 15 Oct. 2021, Japanese, Invited oral presentation
15 Oct. 2021 - 15 Oct. 2021, [Invited]

微生物分解性プラスチックの開発と活用法　　　　　　　　　　　　　　　
松本謙一郎
北海道経済連合会 環境・エネルギーセミナー, 18 Jan. 2021, Japanese, Public discourse
[Invited]

組み換え大腸菌による4-ヒドロキシアルカン酸を含むブロック共重合体PHAの生合成　　　　　　　　　　　　　　　
磯部凪, 富田宏矢, 松本謙一郎
日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2021
2021 - 2021

Biosynthesis of glycolic acid-containing polyhydroxyalkanoates using sequence-controlled enzymes　　　　　　　　　　　　　　　
穂積侑伽, 冨田宏矢, 荒井修造, 榊原早也果, MARESCHAL Robin, 大井俊彦, ZINN Manfred, 松本謙一郎
日本生物工学会大会講演要旨集, 2021
2021 - 2021

Biosynthesis of polyhydroxyalkanoates with block sequence by recombinant Ralstonia eutropha　　　　　　　　　　　　　　　
石原静流, 折田和泉, 松本謙一郎, 福居俊昭
日本農芸化学会大会講演要旨集(Web), 2021
2021 - 2021

Biosynthesis of random-homo block copolymer poly[glycolate-ran-3-hydroxybutyrate (3HB)]-b-poly(3HB) using sequence-regulating chimeric polyhydroxyalkanoate synthase in Escherichia coli　　　　　　　　　　　　　　　
柳川謙吾, 荒井修造, 榊原早也果, MARESCHAL Robin, 大井俊彦, ZINN Manfred, 松本謙一郎
日本農芸化学会大会講演要旨集(Web), 2021
2021 - 2021

Biosynthesis of polyhydroxyalkanoate using RuBisCO pathway and its application to evaluation of RuBisCO　　　　　　　　　　　　　　　
藤原悠暉, 齋藤樹理, 永田暁洋, 大潟祥梧, 二宮太樹, 谷口遼, 松本直己, 堀千明, 大井俊彦, 蘆田弘樹, 松本謙一郎
日本農芸化学会大会講演要旨集(Web), 2021
2021 - 2021

微生物産生ブロック共重合体ポリマーの構造機能相関の解析　　　　　　　　　　　　　　　
佐藤 圭悟、影山 友基、藤井 隆之輔、松本 謙一郎
日本農芸化学会2020年度大会, 27 Mar. 2020, Japanese, Oral presentation

新規配列制御型グリコール酸ポリマーの生合成と構造解析　　　　　　　　　　　　　　　
梶川彩香、榊原早也果、荒井修造、大井俊彦、堀千明、松本謙一郎
日本農芸化学会2020年度大会, 27 Mar. 2020, Japanese, Oral presentation

Culture conditions for biosynthesis of novel lactate-based polymers　　　　　　　　　　　　　　　
岡本沙樹, 西上明花, 後藤早希, 松本謙一郎, 阿部英喜, 田口精一, 田中賢二, 松崎弘美, 松崎弘美
日本農芸化学会大会講演要旨集(Web), 2020
2020 - 2020

ブロックポリヒドロキシアルカン酸の微生物合成と構 造・物性解析　　　　　　　　　　　　　　　
松本 謙一郎, 影山 友基, 堀 千明, 大井 俊彦, 磯野 拓也, 佐藤 敏文, 田口 精一
第68回高分子討論会, 25 Sep. 2019, Japanese, Oral presentation

Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) 反応を経由したグリコール酸ポリマー生合成系とその応用　　　　　　　　　　　　　　　
大潟祥梧、永田暁洋、齋藤樹理、横尾俊憲、工藤悠希、堀千明、大井俊彦、田口精一、松本謙一郎
第71回日本生物工学会大会, 16 Sep. 2019, Japanese, Oral presentation

Ribulose 1,5-bisphosphate carboxylase/oxygenase(RubisCO)反応を経由したグリコール酸ポリマー生合成系構築とその応用　　　　　　　　　　　　　　　
大潟祥梧, 永田暁洋, 齋藤樹理, 横尾俊憲, 工藤悠希, 堀千明, 大井俊彦, 田口精一, 松本謙一郎
日本生物工学会大会講演要旨集, 2019
2019 - 2019

非天然ポリエステル生合成系の開発と配列制御型ポリマーへの展開　　　　　　　　　　　　　　　
松本謙一郎, 松本謙一郎, 松本謙一郎, 堀千明, 大井俊彦, 大井俊彦, 磯野拓也, 佐藤敏文, 石井大輔, 石井大輔, 岩田忠久, 岩田忠久, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2019
2019 - 2019

ルビスコ経路を利用した組換え大腸菌におけるグリコール酸ポリマー生合成系の構築　　　　　　　　　　　　　　　
永田暁洋, 齋藤樹理, 横尾俊憲, 工藤悠希, 堀千明, 大井俊彦, 田口精一, 松本謙一郎, 松本謙一郎
日本農芸化学会大会講演要旨集(Web), 2019
2019 - 2019

X線繊維回折法によるポリ[ (R)-2-ヒドロキシブタン酸]の結晶構造解析　　　　　　　　　　　　　　　
丸林弘典, 金子はるひ, 木村聡, 木村聡, 松本謙一郎, 松本謙一郎, 田口精一, 田口精一, 岩田忠久, 岩田忠久, 岩田忠久
繊維学会予稿集, 2018
2018 - 2018

オリゴ酪酸およびオリゴ乳酸とポリマー分解酵素間の相互作用の分子動力学シミュレーションを用いた検討　　　　　　　　　　　　　　　
織田耕平, 鎌田佑, 松本謙一郎, 大井俊彦, 田口精一, 佐藤信一郎
化学系学協会北海道支部冬季研究発表会(Web), 2018
2018 - 2018

ポリ[ (R)-2-ヒドロキシブタン酸]の結晶構造解析　　　　　　　　　　　　　　　
丸林弘典, 金子はるひ, 木村聡, 木村聡, 松本謙一郎, 松本謙一郎, 田口精一, 田口精一, 岩田忠久, 岩田忠久, 岩田忠久
繊維学会予稿集, 2018
2018 - 2018

組換え大腸菌による乳酸ベースオリゴマーの分泌生産と高分子合成への応用　　　　　　　　　　　　　　　
松本謙一郎, UTSUNOMIA Camila, 堀千明, 田口精一
日本農芸化学会大会講演要旨集(Web), 2018
2018 - 2018

転写因子欠失株を用いた乳酸ベースポリマー生産大腸菌のリモデリング　　　　　　　　　　　　　　　
門屋亨介, 門屋亨介, 児玉悠, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2018
2018 - 2018

透明性を有する新規乳酸ベースポリマーの生合成　　　　　　　　　　　　　　　
後藤早希, 龍野菜々美, 松本謙一郎, 阿部英喜, 田口精一, 田中賢二, 松崎弘美, 松崎弘美
日本農芸化学会大会講演要旨集(Web), 2018
2018 - 2018

モノマー組成制御された乳酸ベースオリゴマーの分泌生産　　　　　　　　　　　　　　　
廣江綾香, 廣江綾香, 増永凛, 佐藤千絵, 松本謙一郎, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2018
2018 - 2018

グルコース/キシロース切替え流加法による乳酸ベースポリマー生産組換え大腸菌の高密度培養　　　　　　　　　　　　　　　
山崎貴史, RIBORDY Greg, 堀千明, 堀千明, 松本謙一郎, 松本謙一郎, 大井俊彦, 大井俊彦, ZINN Manfred, 田口精一, 田口精一
日本生物工学会大会講演要旨集, 2018
2018 - 2018

植物バイオマス原料から微生物合成する多元ポリ乳酸の部材化　　　　　　　　　　　　　　　
田口精一, 田口精一, 田口精一, 廣江綾香, 廣江綾香, 石井大輔, 石井大輔, 大井俊彦, 大井俊彦, 松本謙一郎, 松本謙一郎, 岩田忠久, 岩田忠久
高分子学会予稿集(CD-ROM), 2018
2018 - 2018

動的配列制御に基づくブロック共重合ポリエステル生合成系の発見とポリマー構造解析
松本謙一郎, 藤井隆之輔, 堀千明, 大井俊彦, 磯野拓也, 佐藤敏文, 田口精一
高分子学会予稿集(CD-ROM), 2018, Japanese
2018 - 2018

Direct secretory production of D-lactate oligomers by engineered Escherichia coli: a shortcut in the process of polylactide production　　　　　　　　　　　　　　　
UTSUNOMIA Camila, MATSUMOTO Ken’ichiro, HORI Chiaki, TAGUCHI Seiichi, MATSUMOTO Ken’ichiro, HORI Chiaki, TAGUCHI Seiichi
日本生物工学会大会講演要旨集, 2017
2017 - 2017

配向結晶化フィルム及び単結晶を用いたポリ[ (R)-2-ヒドロキシブチレート]の結晶構造および高次構造解析　　　　　　　　　　　　　　　
金子はるひ, 石井大輔, 石井大輔, 石井大輔, 木村聡, 木村聡, 加部泰三, 加部泰三, 加部泰三, 加部泰三, 引間孝明, 高田昌樹, 松本謙一郎, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一, 岩田忠久, 岩田忠久, 岩田忠久
繊維学会予稿集, 2017
2017 - 2017

転写因子欠失株を用いた乳酸ベースポリマー生産大腸菌のリモデリング　　　　　　　　　　　　　　　
門屋亨介, 門屋亨介, 児玉悠, 松本謙一郎, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一
日本生物工学会大会講演要旨集, 2017
2017 - 2017

Lactyl-CoA重合における乳酸重合酵素の反応機構解析　　　　　　　　　　　　　　　
飯島翠, 松本謙一郎, 松本謙一郎, 堀千明, 堀千明, 田口精一, 田口精一, 田口精一, 大井俊彦, 大井俊彦
日本生物工学会大会講演要旨集, 2017
2017 - 2017

組換え大腸菌による新規モノマー組成からなる乳酸ベースポリマーの生合成　　　　　　　　　　　　　　　
後藤早希, 外村彩夏, 田口精一, 松本謙一郎, 阿部英喜, 田中賢二, 松崎弘美, 松崎弘美
日本生物工学会大会講演要旨集, 2017
2017 - 2017

ポリ(乳酸-co-3-ヒドロキシ酪酸)フィルムの柔軟性に及ぼす結晶性と熱物性の影響　　　　　　　　　　　　　　　
石井大輔, 石井大輔, 石井大輔, 石井大輔, 滝沢憲治, 滝沢憲治, 松本謙一郎, 松本謙一郎, 大井俊彦, 大井俊彦, 引間孝明, 高田昌樹, 岩田忠久, 岩田忠久, 岩田忠久, 田口精一, 田口精一, 田口精一
日本生物工学会大会講演要旨集, 2017
2017 - 2017

パルプ製造廃液を炭素源とした乳酸ベースポリマーの生産　　　　　　　　　　　　　　　
大井俊彦, 大井俊彦, 滝沢憲治, 滝沢憲治, 松本謙一郎, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2017
2017 - 2017

非天然ポリステル合成のための重合酵素の高活性化　　　　　　　　　　　　　　　
松本謙一郎, 松本謙一郎, 大石健太, 大井俊彦, 大井俊彦, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2017
2017 - 2017

組換え大腸菌による新規乳酸ベースポリマーの生合成　　　　　　　　　　　　　　　
後藤早希, 留野菜月, 外村彩夏, 田口精一, 松本謙一郎, 阿部英喜, 田中賢二, 松崎弘美, 松崎弘美
日本農芸化学会大会講演要旨集(Web), 2017
2017 - 2017

組換え大腸菌による乳酸ポリマー生産のためのキシロースを炭素源とした高密度培養　　　　　　　　　　　　　　　
滝沢憲治, 滝沢憲治, RIBORDY Greg, 山崎貴史, 松本謙一郎, 大井俊彦, 大井俊彦, ZINN Manfred, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2017
2017 - 2017

新規の生分解性乳酸ベースポリマーの生合成　　　　　　　　　　　　　　　
後藤早希, 外村彩夏, 田口精一, 松本謙一郎, 阿部英喜, 田中賢二, 松崎弘美
化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集, 2017
2017 - 2017

昆虫由来抗菌ペプチドの高活性化と作用機序解明のための新アプローチ　　　　　　　　　　　　　　　
松本謙一郎, 山崎蔵亨, 川上駿, 三吉大地, 堀千明, 田口精一, 田口精一, 大井俊彦, 橋本茂樹
日本生物工学会大会講演要旨集, 2017, (公社)日本生物工学会, Japanese
2017 - 2017

抗菌ペプチド・アピデシンおよび高活性変異体の作用機構解析　　　　　　　　　　　　　　　
川上駿, 山崎蔵亨, 三吉大地, 大井俊彦, 橋本茂樹, 堀千明, 田口精一, 田口精一, 松本謙一郎
日本生物工学会大会講演要旨集, 2017, (公社)日本生物工学会, Japanese
2017 - 2017

Mechanistic analysis of poly(lactate-co-3-hydroxybutyrate) degradation by soil bacterium depolymerase　　　　　　　　　　　　　　　
UTSUNOMIA Camila, SUN Jian, MATSUMOTO Ken’ichiro, OOI Toshihiko, OOI Toshihiko, TAGUCHI Seiichi, TAGUCHI Seiichi
日本農芸化学会大会講演要旨集(Web), 2016
2016 - 2016

Escherichia coliを用いた乳酸ベースバイオポリマー生産の検討　　　　　　　　　　　　　　　
滝沢憲治, 大井俊彦, 松本謙一郎, 田口精一
農業食料工学会年次大会講演要旨, 2016
2016 - 2016

加水分解性を有するグリコール酸ベースポリマーの微生物合成　　　　　　　　　　　　　　　
工藤悠希, 平出幸和, 大井俊彦, 大井俊彦, 田口精一, 田口精一, 松本謙一郎, 松本謙一郎
日本農芸化学会大会講演要旨集(Web), 2016
2016 - 2016

ジャーファメンターを用いた組換えE.coliによる乳酸ベースバイオポリマー生産法の確立　　　　　　　　　　　　　　　
滝沢憲治, 滝沢憲治, 大井俊彦, 大井俊彦, 松本謙一郎, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2016
2016 - 2016

バイオマスを利用した非天然バイオプラスチックの生合成　　　　　　　　　　　　　　　
松本謙一郎, 松本謙一郎, 門屋亨介, 門屋亨介, 滝沢憲治, 滝沢憲治, 大井俊彦, 大井俊彦, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2016
2016 - 2016

組換え大腸菌による乳酸ユニットを含む新規モノマー組成からなる生分解共重合ポリエステルの生合成　　　　　　　　　　　　　　　
後藤早希, 中上美歩, 外村彩夏, 田口精一, 松本謙一郎, 田中賢二, 松崎弘美, 松崎弘美
日本農芸化学会大会講演要旨集(Web), 2016
2016 - 2016

ゲノム工学を用いた物質生産微生物工場開発の新戦略　　　　　　　　　　　　　　　
門屋亨介, 門屋亨介, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一
日本農芸化学会大会講演要旨集(Web), 2016
2016 - 2016

乳酸ユニットを含む新規モノマー組成からなる生分解性プラスチックの生合成　　　　　　　　　　　　　　　
後藤早希, 外村彩夏, 田口精一, 松本謙一郎, 阿部英喜, 田中賢二, 松崎弘美
化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集, 2016
2016 - 2016

ポリ乳酸およびポリヒドロキシ酪酸単分子溶液の分子動力学シミュレーション:バイオポリエステル分解酵素の機構解明を目指して　　　　　　　　　　　　　　　
鎌田佑, 水谷駿介, 佐藤信一郎, 佐藤信一郎, 覚知豊次, 覚知豊次, 孫健, 松本謙一郎, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一
高分子学会北海道支部研究発表会講演要旨集, 2016
2016 - 2016

ポリ[ (R)-2-ヒドロキシブチレート]の基礎物性,結晶構造および酵素分解性　　　　　　　　　　　　　　　
牧野恭平, 岩田忠久, 岩田忠久, 岩田忠久, 加部泰三, 加部泰三, 加部泰三, 加部泰三, 松本謙一郎, 松本謙一郎, 田口精一, 田口精一, 引間孝明, 高田昌樹
高分子学会予稿集(CD-ROM), 2016
2016 - 2016

ポリ(乳酸-co-3-ヒドロキシ酪酸)の結晶化挙動および力学物性におけるモノマー組成の影響　　　　　　　　　　　　　　　
石井大輔, 石井大輔, 石井大輔, 岩田忠久, 岩田忠久, 岩田忠久, 滝沢憲治, 滝沢憲治, 松本謙一郎, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一, 引間孝明, 高田昌樹
高分子学会予稿集(CD-ROM), 2016
2016 - 2016

ポリ(乳酸-co-3-ヒドロキシ酪酸)ナノファイバーの作製と細胞培養基材への応用　　　　　　　　　　　　　　　
石井大輔, 石井大輔, 木村聡, 岩田忠久, 岩田忠久, 松本謙一郎, 松本謙一郎, 田口精一, 田口精一
繊維学会予稿集, 2016
2016 - 2016

ゲノム工学を用いた細胞内蓄積型物質生産に有益な宿主の開発　　　　　　　　　　　　　　　
門屋亨介, 門屋亨介, 松本謙一郎, 大井俊彦, 田口精一, 田口精一
日本ゲノム微生物学会年会要旨集, 2016
2016 - 2016

木質抽出液の加水分解物を原料とした乳酸ベースポリマー生産　　　　　　　　　　　　　　　
滝沢憲治, 滝沢憲治, 大井俊彦, 大井俊彦, 松本謙一郎, 田口精一, 田口精一
日本農芸化学会北海道支部講演会講演要旨, 2016
2016 - 2016

転写因子欠失株を用いた乳酸ベースポリマー生産大腸菌のリモデリング　　　　　　　　　　　　　　　
門屋亨介, 門屋亨介, 児玉悠, 松本謙一郎, 大井俊彦, 大井俊彦, 田口精一, 田口精一
日本農芸化学会北海道支部講演会講演要旨, 2016
2016 - 2016

乳酸ユニットを含む新規モノマー組成からなる生分解共重合ポリエステルの生合成
後藤 早希, 外村 彩夏, 西村 綾乃, 田口 精一, 松本 謙一郎, 田中 賢二, 松崎 弘美
日本生物工学会大会講演要旨集, Sep. 2015, (公社)日本生物工学会, Japanese
Sep. 2015 - Sep. 2015

遺伝子組換え大腸菌を用いた乳酸ベースポリマーの生合成
川上 恭平, 廣江 綾香, 松本 謙一郎, 田口 精一, 柘植 丈治
日本生物工学会大会講演要旨集, Sep. 2015, (公社)日本生物工学会, Japanese
Sep. 2015 - Sep. 2015

乳酸ポリマーを高蓄積する微生物工場の創製とポリマー高機能化
MATSUMOTO KEN'ICHIRO, UTSUNOMIA CAMILA, KADOYA RYOSUKE, KADOYA RYOSUKE, SUN JIAN, NDUKO JOHN M, ISHII DAISUKE, ISHII DAISUKE, KABE TAIZO, KABE TAIZO, KOIZUMI ARISA, OI TOSHIHIKO, OI TOSHIHIKO, IWATA TADAHISA, IWATA TADAHISA, TAGUCHI SEIICHI, TAGUCHI SEIICHI
高分子学会予稿集(CD-ROM), 25 Aug. 2015, Japanese
25 Aug. 2015 - 25 Aug. 2015

ポリ[ (R)‐2‐ヒドロキシブチレート]に対する結晶構造解析の試み　　　　　　　　　　　　　　　
MAKINO KYOHEI, KIMURA SATOSHI, TAKEMURA AKIO, KABE TAIZO, IWATA TADAHISA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, HIKIMA TAKAAKI, TAKATA MASAKI
繊維学会予稿集, 08 Jun. 2015, Japanese

様々な新規微生物産性ポリエステルの熱物性と光学特性　　　　　　　　　　　　　　　
KABE TAIZO, DANJIYO TAKAHIRO, IWATA TADAHISA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, TSUGE TAKEHARU, NOBUKAWA SHOGO, YAMAGUCHI MASAYUKI
高分子学会予稿集(CD-ROM), 12 May 2015, Japanese

ポリ[ (R)‐2‐ヒドロキシブチレート]の結晶構造解析　　　　　　　　　　　　　　　
MAKINO KYOHEI, KIMURA SATOSHI, TAKEMURA AKIO, KABE TAIZO, IWATA TADAHISA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, HIKIMA TAKAAKI, TAKATA MASAKI
高分子学会予稿集(CD-ROM), 12 May 2015, Japanese

組換え大腸菌による糖からの生分解性共重合ポリエステルの生合成　　　　　　　　　　　　　　　
HOKAMURA AYAKA, NISHIMURA AYANO, GOTO SAKI, WAKIDA IZUMI, TAGUCHI SEIICHI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TANAKA KENJI, MATSUSAKI HIROMI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015, Japanese

組換え大腸菌を用いた高光学活性(R)‐3‐ヒドロキシブタン酸の生産におけるCoA転移酵素高活性変異体の効果　　　　　　　　　　　　　　　
MIYOSHI DAICHI, SETO NOZOMI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015, Japanese

組換え大腸菌を利用した高光学純度(S)‐3‐ヒドロキシブタン酸の生産　　　　　　　　　　　　　　　
SETO NOZOMI, OI TOSHIHIKO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015, Japanese

耐酸性乳酸菌Lactobacillus acetotolerans HTの乳酸脱水素酵素遺伝子のクローニングと生分解性共重合ポリエステルの生合成　　　　　　　　　　　　　　　
GOTO SAKI, HOKAMURA AYAKA, TAGUCHI SEIICHI, MATSUMOTO KEN'ICHIRO, TANAKA KENJI, MATSUSAKI HIROMI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015, Japanese

大腸菌転写制御因子欠失株を用いたP(LA‐co‐3HB)の高生産化　　　　　　　　　　　　　　　
KODAMA YU, KADOYA RYOSUKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015, Japanese

組換え大腸菌によるグリコール酸ベースポリマー合成とポリマーの物性評価　　　　　　　　　　　　　　　
HIRAIDE YUKIKAZU, SHIBA TETSUFUMI, SUN JIAN, OI TOSHIHIKO, TAGUCHI SEIICHI, MATSUMOTO, KEN'ICHIRO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015, Japanese

組み換え体大腸菌で生産する資源循環型微生物ポリマーの高生産化に関与する新規遺伝子の探索　　　　　　　　　　　　　　　
KADOYA RYOSUKE, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015, Japanese

3-ヒドロキシ酪酸および乳酸をモノマーユニットに含む生分解性ポリエステルナノファイバーの作製と特性解析　　　　　　　　　　　　　　　
石井大輔, 石井大輔, 加部泰三, 加部泰三, NDUKO John, 松本謙一郎, 松本謙一郎, 田口精一, 田口精一, 木村聡, 岩田忠久, 岩田忠久
高分子学会予稿集(CD-ROM), 2015
2015 - 2015

配向結晶性フィルム及び単結晶を用いたポリ[ (R)-2-ヒドロキシブチレート]の結晶構造解析　　　　　　　　　　　　　　　
牧野恭平, 木村聡, 竹村彰夫, 加部泰三, 岩田忠久, 松本謙一郎, 田口精一, 引間孝明, 高田昌樹
高分子学会予稿集(CD-ROM), 2015
2015 - 2015

組換え大腸菌を用いたグリコール酸ベースポリマーの生合成および加水分解性評価　　　　　　　　　　　　　　　
平出幸和, 斯波哲史, 田口精一, 松本謙一郎
日本農芸化学会北海道支部講演会講演要旨, 2015
2015 - 2015

組換え大腸菌を用いたグリコール酸ベースポリマーの生合成と酵素分解性　　　　　　　　　　　　　　　
HIRAIDE YUKIKAZU, SHIBA TETSUFUMI, SU JIAN, OI TOSHIHIKO, TAGUCHI SEIICHI, MATSUMOTO, KEN'ICHIRO
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

生合成乳酸ベースポリマーの高生産化を目指した宿主大腸菌の遺伝子改変　　　　　　　　　　　　　　　
KODAMA YU, KADOYA RYOSUKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

進化工学的手法によるポリヒドロキシアルカン酸合成酵素の乳酸重合能力の強化　　　　　　　　　　　　　　　
AOKI SHUNSUKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

Lactobacillus acetotolerans HTの乳酸脱水素酵素遺伝子を利用した乳酸ユニットを含む生分解性プラスチックの生合成　　　　　　　　　　　　　　　
GOTO SAKI, SATO MISAKI, TANAKA KENJI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, MATSUSAKI HIROMI
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

乳酸ポリマー合成コリネ菌の代謝解析に基づく高生産化　　　　　　　　　　　　　　　
TOBITANI KOTA, SASAMORI TETSUYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

グリコール酸ポリマーの生合成経路の構築　　　　　　　　　　　　　　　
YOKOO TOSHINORI, HIRAIDE YUKIKAZU, TAGUCHI SEIICHI, MATSUMOTO, KEN'ICHIRO
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

二酸化炭素からプラスチックを合成する人工生命システムの創製―最短の合成ルートは?―　　　　　　　　　　　　　　　
MATSUMOTO KEN'ICHIRO, YOKOYAMA TOSHINORI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

ゲノム工学的手法を用いた微生物ポリマー高生産化宿主の創成　　　　　　　　　　　　　　　
KADOYA RYOSUKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

高分子量2‐ヒドロキシブタン酸ベースポリマーの生合成および物性解析　　　　　　　　　　　　　　　
TAKAYA MASAHIRO, OBA TAKASHI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese

微生物産生ポリ[ (R)‐ラクテート‐co‐(R)‐2‐ヒドロキシブチレート]の結晶性および熱的性質　　　　　　　　　　　　　　　
KABE TAIZO, IWATA TADAHISA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, MARUBAYASHI HIRONORI, HIKIMA TAKAAKI, TAKATA MASAKI
高分子学会予稿集(CD-ROM), 09 May 2014, Japanese

植物バイオマス由来の単糖混合物を炭素源としたバイオポリエステルの生産　　　　　　　　　　　　　　　
OI TOSHIHIKO, SASAKI SHOHEI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014, Japanese

微生物を利用したキラルP(2‐ヒドロキシ酪酸)の合成とポリマー解析　　　　　　　　　　　　　　　
MIYAKE MASAHIRO, MATSUMOTO KEN'ICHIRO, TERAI SATSUKI, KABE TAIZO, IWATA TADAHISA, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014, Japanese

バイオマスから高効率で乳酸プラスチックを生産する微生物工場の開発とポリマー物性評価　　　　　　　　　　　　　　　
MATSUMOTO KEN'ICHIRO, MAEDA MICHIHISA, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014, Japanese

ポリ[ (R)‐2‐ヒドロキシブチレート]の基礎物性評価と構造解析の試み　　　　　　　　　　　　　　　
MAKINO KYOHEI, KABE TAIZO, TAKEMURA AKIO, IWATA TADAHISA, TAGUCHI SEIICHI, MATSUMOTO KEN'ICHIRO, HIKIMA TAKAAKI, TAKATA MASAKI, MARUBAYASHI HIRONORI
繊維学会予稿集, 2014, Japanese

組換えコリネ型細菌によるポリ乳酸様ポリマー生産の増強　　　　　　　　　　　　　　　
TOBITANI KOTA, SASAMORI TETSUYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 27 Nov. 2013, Japanese

抗菌スペクトルを改変した抗菌ペプチド「アピデシン」特殊変異体の創成とその抗菌活性　　　　　　　　　　　　　　　
YAMAZAKI KURATO, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 27 Nov. 2013, Japanese

コリネ菌の細胞表層提示技術を用いたデンプンからの乳酸様ポリマーの生産　　　　　　　　　　　　　　　
KADOYA KYOSUKE, SONG YUYANG, TOBITANI KOTA, MATSUMOTO KEN'ICHIRO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 27 Nov. 2013, Japanese

微生物重合系に基づく乳酸ポリマーからの拡張　　　　　　　　　　　　　　　
TAGUCHI SEIICHI, MATSUMOTO, KEN'ICHIRO
高分子学会予稿集(CD-ROM), 28 Aug. 2013, Japanese

不飽和モノマーを導入した2‐ヒドロキシブタン酸ベースポリマーの生合成とその応用　　　　　　　　　　　　　　　
CHOI YOONGYOO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese

ポリヒドロキシアルカン酸重合酵素の機能改変によるポリマー構造制御　　　　　　　　　　　　　　　
MATSUMOTO KEN'ICHIRO, OCHI ANNA, OBA TAKASHI, TAKAYA MASAHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese

植物バイオマスを用いた組換え大腸菌による共重合ポリエステルの生産　　　　　　　　　　　　　　　
SASAKI SHOHEI, OI TOSHIHIKO, NDUKO JOHN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese

デンプンを炭素源に用いたポリ乳酸様ポリマーの合成　　　　　　　　　　　　　　　
KADOYA RYOSUKE, SONG YUYANG, TOBITANI KOTA, MATSUMOTO KEN'ICHIRO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese

糖を利用した組換え大腸菌内でのグリコール酸ベースポリマーの生産　　　　　　　　　　　　　　　
SHIBA TETSUFUMI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese

非可食性植物バイオマスからのP(3HB)の微生物生産　　　　　　　　　　　　　　　
OI TOSHIHIKO, SASAKI SHOHEI, NDUKO JOHN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese

2‐ヒドロキシブタン酸ベースポリマーの微生物合成とその物性解析　　　　　　　　　　　　　　　
MIYAKE MASAHIRO, TERAI SATSUKI, MATSUMOTO KEN'ICHIRO, KABE TAIZO, IWATA TADAHISA, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese

Corynebacterium glutamicumをプラットフォームとしたポリ乳酸様ポリマー合成系の効率化のための代謝工学および培養工学的アプローチ　　　　　　　　　　　　　　　
TOBITANI KOTA, SONG YUYANG, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese

改変型重合酵素による2‐ヒドロキシブタン酸ベースポリマーの生合成と物性解析　　　　　　　　　　　　　　　
AOKI SHUNSUKE, OBA TAKASHI, OCHI ANNA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese

動力学的解析および立体構造に基づいた進化型アセトアセチル‐CoAレダクターゼの高活性化メカニズムの解明　　　　　　　　　　　　　　　
WATANABE TSUYOSHI, MATSUMOTO KEN'ICHIRO, MOTOHASHI REN, IKEDA KOJI, TANAKA YOSHIKAZU, MIN YAO, TANAKA ISAO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese

微生物ポリエステル合成系酵素の機能改変と多元ポリ乳酸の創製　　　　　　　　　　　　　　　
MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese

PLAを蓄積した大腸菌の透過型電子顕微鏡観察　　　　　　　　　　　　　　　
WAKABAYASHI AIKO, SATO MICHIO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI, MAEDA MICHIHISA
日本顕微鏡学会関東支部講演会予稿集, 05 Mar. 2013, Japanese

PhaB高活性体を用いた光学活性(R)‐3‐ヒドロキシブタン酸生産の向上　　　　　　　　　　　　　　　
OI TOSHIHIKO, HONMA IORI, WATANABE TSUYOSHI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese

Ralstonia eutropha由来PHA重合酵素の改変による2‐ヒドロキシブタン酸重合能力の強化　　　　　　　　　　　　　　　
OCHI ANNA, OBA TAKASHI, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese

Ralstonia eutropha由来ポリヒドロキシアルカン酸重合酵素の改変によるClass I乳酸重合酵素の創出　　　　　　　　　　　　　　　
OCHI ANNA, OBA TAKASHI, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 01 Nov. 2012, Japanese

進化工学的改変による高活性PhaBの速度論的解析とPHB生産性の向上　　　　　　　　　　　　　　　
WATANABE TAKESHI, SO IKUYO, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 01 Nov. 2012, Japanese

セルロース系糖質バイオマスを原料とした高乳酸分率ポリマーの効率的生産　　　　　　　　　　　　　　　
SASAKI KATSUHEI, NDUKO JOHN MASANI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 01 Nov. 2012, Japanese

可変領域への変異導入とその組み合わせによる抗菌ペプチド「アピデシン」の抗菌スペクトル改変　　　　　　　　　　　　　　　
MIYAKE MASAHIRO, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 01 Nov. 2012, Japanese

糖質バイオマスから多様なポリエステルを生産するコリネ菌微生物工場の開発　　　　　　　　　　　　　　　
SONG YUYANG, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2012, Japanese

2‐ヒドロキシブタン酸ベース新奇バイオプラスチックの微生物合成と物性解析　　　　　　　　　　　　　　　
TERAI SATSUKI, ISHIYAMA AYAKO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2012, Japanese

CoA転移酵素を用いた大腸菌による3‐ヒドロキシブタン酸の生産　　　　　　　　　　　　　　　
OI TOSHIHIKO, MATSUMOTO KEN'ICHIRO, OKEI TAKEHIRO, HONMA INORI, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2012, Japanese

乳酸重合活性を有するClass Iポリヒドロキシアルカン酸(PHA)重合酵素の創出　　　　　　　　　　　　　　　
OBA TAKASHI, OCHI ANNA, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2012, Japanese

Microbial Plastic Factory : ポリ乳酸から多元ポリ乳酸の時代へ(バイオリファイナリーの今,そして未来,<特集>バイオ技術10年の軌跡,創立90周年記念特別企画)　　　　　　　　　　　　　　　
松本 謙一郎, 田口 精一
生物工学会誌 : seibutsu-kogaku kaishi, 25 Jul. 2012, Japanese

抗菌ペプチド「アピデシン」の可変領域への変異導入とその抗菌活性への影響　　　　　　　　　　　　　　　
MIYAKE MASAHIRO, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2012, Japanese

進化工学による新規乳酸重合酵素の探索　　　　　　　　　　　　　　　
OCHI ANNA, OBA TAKASHI, SAKAI KOHEI, MATSUMOTO KEN'ICHIRO, TSUGE TAKEHARU, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2012, Japanese

進化工学的改変により得られたアセトアセチル‐CoAレダクターゼ高活性変異体の解析　　　　　　　　　　　　　　　
WATANABE TSUYOSHI, HAYASAKA SATOSHI, IKEDA KOJI, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2011, Japanese

進化型PhaBによる組換えタバコにおけるPHB生産性の増強　　　　　　　　　　　　　　　
OBA TAKASHI, EGUCHI AYA, MORIMOTO KENJIRO, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2011, Japanese

組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成　　　　　　　　　　　　　　　
ISHIYAMA AYAKO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2011, Japanese

進化工学的手法による高乳酸分率のポリ(乳酸‐co‐3‐ヒドロキシブタン酸)が合成可能な乳酸重合酵素の探索　　　　　　　　　　　　　　　
TERAI SATSUKI, SHOZUI FUMI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2011, Japanese

真菌に対する抗菌ペプチドThanatin誘導体の作用評価　　　　　　　　　　　　　　　
OSHIMA YU, SAITO JUNKI, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2011, Japanese

One-pot Production of Lactate-Based Polyesters Using Engineered Microbes Expressing Lactate-Polymerizing Enzyme　　　　　　　　　　　　　　　
MATSUMOTO Ken'ichiro, TAGUCHI Seiichi
KOBUNSHI RONBUNSHU, 25 May 2011, Japanese

高乳酸分率のポリ(乳酸‐co‐3‐ヒドロキシブタン酸)[P(LA‐co‐3HB)]が合成可能な乳酸重合酵素の探索　　　　　　　　　　　　　　　
TERAI SATSUKI, SHOZUI AYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2011, Japanese

高活性化PhaBの導入による組換えタバコでのPHB生産性の向上　　　　　　　　　　　　　　　
OBA TAKASHI, EGUCHI AYA, MORIMOTO KENJIRO, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2011, Japanese

抗菌ペプチド「アピデシン」のN末端領域分子デザインによる高活性変異体の作製と抗菌スペクトル評価　　　　　　　　　　　　　　　
WAKABAYASHI MASAAKI, IWAMURA YUTA, MATSUMOTO KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2011, Japanese

乳酸ベース共重合体P(lactate‐co‐3‐hydroxybutyrate‐co‐3‐hydroxyvalerate)の生合成および物性評価　　　　　　　　　　　　　　　
SON KEN, MOTOHASHI REN, SHOZUI AYA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2011, Japanese

PHA生合成系におけるモノマー供給酵素アセトアセチル‐CoAレダクターゼの高活性変異体の酵素反応解析　　　　　　　　　　　　　　　
WATANABE TAKESHI, HAYASAKA SATOSHI, IKEDA KOJI, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2011, Japanese

抗菌ペプチドThanatin誘導体の酵母様真菌に対する抗菌活性　　　　　　　　　　　　　　　
OSHIMA YU, SAITO JUNKI, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2011, Japanese

Poly(lactate‐co‐3‐hydroxybutyrate‐co‐3‐hydroxyvalerate)の生合成および物性評価　　　　　　　　　　　　　　　
WATANABE TAKESHI, MOTOHASHI TADASHI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
高分子学会北海道支部研究発表会講演要旨集, 01 Feb. 2011, Japanese

グルコースを炭素源とした乳酸ベースポリマーの微生物生産　　　　　　　　　　　　　　　
AIDA AOI, SO IKUYO, MATSUMOTO KEN'ICHIRO, YAMADA MIWA, TAGUCHI SEIICHI
高分子学会北海道支部研究発表会講演要旨集, 01 Feb. 2011, Japanese

アミノ酸残基置換による抗菌ペプチド「アピデシン」の抗菌スペクトルの改変　　　　　　　　　　　　　　　
IWAMURA YUTA, YAMAGATA KYOKO, WAKABAYASHI MASAAKI, MATSUMOTO, KEN'ICHIRO, HASHIMOTO SHIGEKI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 2011, Japanese

高乳酸分率ポリ(乳酸‐co‐3‐ヒドロキシブタン酸)合成のための乳酸重合酵素の進化工学的改変　　　　　　　　　　　　　　　
SAKAI KOHEI, TERAI SATSUKI, SHOZUI AYA, OCHI ANNA, MATSUMOTO, KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 2011, Japanese

乳酸およびグリコール酸ベース新奇バイオプラスチックの微生物生産　　　　　　　　　　　　　　　
SHIBA TETSUSHI, ISHIYAMA AYAKO, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 2011, Japanese

組換え大腸菌を利用した(R)‐3‐ヒドロキシ酪酸の生産　　　　　　　　　　　　　　　
OKEI GAKUYO, HONMA INORI, MATSUMOTO KEN'ICHIRO, WATANABE TAKESHI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会北海道支部講演会講演要旨, 2011, Japanese

Pseudomonas sp.61‐3由来PHA重合酵素の新規高活性変異体の取得と機能解析　　　　　　　　　　　　　　　
SAKAI KOHEI, SHOZUI FUMI, MATSUMOTO KEN'ICHIRO, TAKASE KAZUMA, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2010, Japanese

抗菌ペプチドThanatinのシステイン部位に導入されたアルキル基の側鎖長が抗菌活性に及ぼす影響　　　　　　　　　　　　　　　
SAITO JUNKI, ORIKASA YOSHITAKE, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2010, Japanese

N末分子デザインによる抗菌ペプチド「アピデシン」の高活性体創出　　　　　　　　　　　　　　　
YAMAGATA KYOKO, HASHIMOTO SHIGEKI, ORIKASA YOSHITAKE, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2010, Japanese

組換え大腸菌を用いたポリ(乳酸‐co‐3‐ヒドロキシブタン酸‐co‐3‐ヒドロキシヘキサン酸)の生産　　　　　　　　　　　　　　　
SHOZUI FUMI, MOTOHASHI REN, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2010, Japanese

ポリヒドロキシアルカン酸生合成系のモノマー供給酵素acetoacetyl‐CoA reductaseの進化工学的改変　　　　　　　　　　　　　　　
HAYASAKA SATOSHI, MOTOHASHI REN, SHOZUI FUMI, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Sep. 2010, Japanese

新奇かつデザイナブルなバイオポリエステル合成のための重合酵素の機能改変　　　　　　　　　　　　　　　
MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本蛋白質科学会年会プログラム・要旨集, 15 May 2010, Japanese

3‐ヒドロキシブタン酸ベースバイオポリマーの組成制御を目指したモノマー供給酵素の進化工学的改変　　　　　　　　　　　　　　　
SHOZUI AYA, MOTOHASHI REN, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2010, Japanese

ルテニウム触媒によるセルロース糖化物を用いたポリヒドロキシブタン酸(PHB)の微生物生産　　　　　　　　　　　　　　　
IKEDA KOJI, MATSUMOTO KEN'ICHIRO, KOMANOYA TASUKU, KOBAYASHI HIROKAZU, OI TOSHIHIKO, FUKUOKA ATSUSHI, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2010, Japanese

乳酸重合酵素への新規変異導入による乳酸コポリマーの分率制御　　　　　　　　　　　　　　　
YAMADA MIWA, SHIMIZU KOTARO, URAMOTO SHU, NAKAI TAKANORI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2010, Japanese

ジャーファーメンターを用いた乳酸ベースポリマーの微生物発酵生産　　　　　　　　　　　　　　　
URAMOTO SHU, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, ABE HIDEKI, OI TOSHIHIKO, TAGUCHI SEIICHI
日本農芸化学会大会講演要旨集, 05 Mar. 2010, Japanese

3‐ヒドロキシ吉草酸ユニットを含む新規乳酸ベース3元共重合体の微生物合成　　　　　　　　　　　　　　　
MASAMIZU FUMI, NAKAI TAKANORI, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
高分子学会北海道支部研究発表会講演要旨集, 26 Jan. 2010, Japanese

立体選択的乳酸ベースポリマーのバイオ合成と構造解析　　　　　　　　　　　　　　　
YAMADA MIWA, NAKAI TAKANORI, SHIMIZU KOTARO, URAMOTO SHU, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
高分子学会北海道支部研究発表会講演要旨集, 26 Jan. 2010, Japanese

担持ルテニウム触媒によるセルロース糖化反応を利用したポリヒドロキシブタン酸(PHB)の微生物生産　　　　　　　　　　　　　　　
MATSUMOTO KEN'ICHIRO, IKEDA KOJI, KOMANOYA TASUKU, KOBAYASHI HIROKAZU, FUKUOKA ATSUSHI, TAGUCHI SEIICHI
日本生物工学会シンポジウム講演要旨集, 2010, Japanese

乳酸ポリマー生産用微生物工場の開発　　　　　　　　　　　　　　　
TAGUCHI SEIICHI, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, NAKAI TAKANORI, SHIMIZU KOTARO
高分子学会予稿集(CD-ROM), 01 Sep. 2009, Japanese

組換え大腸菌を用いた新規乳酸ベース3元共重合体の生産　　　　　　　　　　　　　　　
SHOZUI FUMI, NAKAI TAKANORI, YAMADA MIWA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2009, Japanese

抗菌ペプチドThanatinおよびその高活性化学修飾体の細菌に対する作用　　　　　　　　　　　　　　　
ORIKASA YOSHITAKE, SAITO JUNKI, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2009, Japanese

Bacillus sp.B29由来アゾ還元酵素基質複合体の結晶構造解析　　　　　　　　　　　　　　　
OGATA DAIKI, TAGUCHI SEIICHI, MATSUMOTO KEN'ICHIRO, FUJIWARA TAKAAKI, TANAKA ISAO, YAO MIN, OI TOSHIHIKO
日本生物工学会大会講演要旨集, 25 Aug. 2009, Japanese

抗菌ペプチドApidaecinの高活性化のための一戦略:細胞内導入量の向上　　　　　　　　　　　　　　　
YAMAGATA KYOKO, ICHINOHE KENTA, ORIKASA YOSHITAKE, HASHIMOTO SHIGEKI, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TAGUCHI SEIICHI
日本生物工学会大会講演要旨集, 25 Aug. 2009, Japanese

Microbial Factory for the Production of Bioplastics　　　　　　　　　　　　　　　
YAMADA MIWA, MATSUMOTO KEN'ICHIRO, TAGUCHI SEIICHI
Fiber, 10 Nov. 2008, Japanese

PHA synthase engineering toward super biocatalysts for custom-made biopolymers　　　　　　　　　　　　　　　
MATSUMOTO Kenichiro, TAGUCHI Seiichi
Bioscience & industry, 01 Jul. 2007, Japanese

Biosynthesis of sequence-regulated biodegradable polyesters and analysis of the mechanism of synthesis
Grants-in-Aid for Scientific Research
01 Apr. 2020 - 31 Mar. 2025
松本 謙一郎, 冨田 宏矢, 大井 俊彦
微生物産生ポリエステルであり生分解性プラスチックとして利用できるポリヒドロキシアルカン酸（PHA）の新規構造制御法を開発した。PHAの構造制御は、材料物性に直結する重要な技術課題である。申請者が独自に見出したPHA合成酵素により、非天然モノマーであるグリコール酸を含むブロック共重合体をポリマーを得ることができた。得られた共重合体は、同一分子内にランダム共重合体とホモポリマーセグメントを有する、特殊な配列構造を有していた。本成果はFrontiers in Bioengineering and Biotechnology誌で発表した。さらに、ブロック共重合体の化学構造の拡張に取り組んだ。使用している配列制御型重合酵素は、3-hydroxyhexanoate (3HHx)モノマーに弱い活性を有する。3HHxは材料に柔軟性を付与する性質がある事が知られ、PHAの重要な構成成分の一つである。そこで、進化工学的手法を用いて3HHxモノマーに対する活性が向上した変異体を創出した。こうして得られた新たな重合酵素を用いることで、3HHxモノマーから構成される新規ブロック共重合体を合成することができた。さらに本成果を構造類縁体である6-hydroxyhexanoate (6HHx)に応用し、これまでで最も6HHx分率の高いポリマーを合成することができた。これらの成果はBiomacromolecules誌で発表した。獲得した優良変異体を用いて、D型ポリ乳酸を含むブロック共重合体の合成にも初めて成功した（ACS Sus Chem Eng）。ブロック配列の合成機構を解明するため、独自に開発したNMR法およびreal-time NMR法を用いたin vitro解析を行った。その結果、２つのセグメントが合成される順序を明らかにした。本成果はBiophysical Chemistry誌で発表した。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23K20394

Biosynthesis of sequence-regulated polyesters and analysis of synthetic mechanism
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2020 - 31 Mar. 2025
松本 謙一郎, 大井 俊彦
本研究課題は、微生物産生ポリエステルであり生分解性プラスチックとして利用できるポリヒドロキシアルカン酸（PHA）の構造制御に関するものである。PHAの構造制御は、材料物性に直結する重要な技術課題である。PHAは、通常共重合体の合成においてはランダム共重合体しか作ることができないが、申請者はブロック共重合体を合成できる方法を独自に見出している。この合成手法を応用することで、非天然モノマーであるグリコール酸を含むポリマーを得ることができた。得られた共重合体は、同一分子内にランダム共重合体と単独重合体の構造を有する、特殊な配列構造を有していた。ランダム共重合体部分のグリコール酸分率は推定約70モル％に達し。本結果は、これまで全く例のない特殊な構造を持つPHAの合成を可能にするものであり、かつ、グリコール酸分率の高い構造を合成する新規な方法を与えた。グリコール酸ポリマーは高い加水分解性を持つことで知られることから、PHAに加水分解性を付与する効果が期待された。これらの成果はFrontiers in Bioengineering and Biotechnology誌に発表した。さらに、ブロック共重合体の化学構造の拡張に取り組んだ。使用している配列制御型重合酵素は、3-hydroxyhexanoate (3HHx)モノマーに弱い活性を有する。3HHxは材料に柔軟性を付与する性質がある事が知られ、PHAの重要な構成成分の一つである。そこで、進化工学的手法を用いて3HHxモノマーに対する活性が向上した変異体を創出した。こうして得られた新たな重合酵素を用いることで、3HHxモノマーから構成される新規ブロック共重合体を合成することができた。本成果はBiomacromolecules誌に発表した。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 20H04368

Microbial production of elastic polymers with ordered structure
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2017 - 31 Mar. 2020
Matsumoto Ken'ichiro
Bacterial polyester polyhydroxyalkanoates (PHAs) are biobased and biodegradable plastic, which are commercially used in Japan since 2019. To further expand the use of the material, the regulation of physical properties is an important research target. The conventional but effective strategy is random copolymerization, which increases the resistance to impact. We developed a novel method to control the monomer sequence of the polymer. In fact, the obtained sequence-regulated PHAs exhibited superior properties to the conventional polymers. These results indicated the usefulness of the sequence-regulating technique of PHA for creating the next-generation materials.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 17H01902

Creation of new biodegradability controlled plastics based on the degradation mechanism
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2017 - 31 Mar. 2020
Ooi Toshihiko
Marine pollution caused by petroleum-derived plastics has become a global concern. Among them, biodegradable plastics that can be decomposed in the natural environment into water and carbon dioxide are attracting attention. In this research project, we developed a newly developed bioplastic, lactic acid copolymer [P (LA-co-3HB)], which is a polymer molecule composed of lactic acid (LA) and 3-hydroxybutanoic acid (3-HB). And the degradability by environmental microorganisms was evaluated.
First, we investigated the microbial production method of lactic acid copolymer and established a culture method with controlled lactic acid content. The obtained copolymer were formed into a film to evaluate the degradation in the environment, and a phylogenetic analysis of the environmental microorganisms involved in the degradation was performed.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 17K00619

Efficient production of high optical pure hydroxy acid based on the regeneration of coenzyme A
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2014 - 31 Mar. 2017
Ooi Toshihiko, Taguchi Seiichi
Optical purity of building block are essential for the chemical synthesis of bioactive compounds, such as antibiotics and pheromone. Chemical synthesis of optically active compounds are not so easy because of no optical selective synthesis. We developed basic technology of the biosynthesis method for optically active [R]-3-hydroxybutylate (3HB) with high purity using engineered microbiological system.
We have newly developed the quantifying analytical method for intermediate compounds of [R}-3HB biosynthesis and found that the improvement of the flux to Acetyl-CoA is effective to produce [R]-3HB production. On the basis this results Escherichi coli JM109 was selected as a high glucose consumption strains. Using this strain the concentrations of glucose and acetate into the medium was optimized. Productivity of high optically pure [R]-3HB was archived to 15g/l in optimized culture condition. In addition newly cultivation technology was futher developed for the efficient production.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 26420791

Microbial production of elastic polymers with ordered structure
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2014 - 31 Mar. 2017
Matsumoto Ken'ichiro
Microbial polyesters are useful material, which are produced from renewable biomass. For the practical use of the material, polymer properties are critical factors. It is well-known that microbial copolyesters are random polymer. However, we found a condition that generated copolymer with low randomness. This study addressed optimization of the polymer production, structure and property analysis, and kinetic analysis of relevant enzymes. As the results, the copolymer, in which the monomer composition was regulated, was successfully synthesized with good yield. The copolymers exhibit transparent and flexible properties. The enzymatic activity of relevant enzymes accounted for the intracellular intermediate levels. Overall, the study contributed to the significantly improved the efficiency of the polymer production system, and provided insight into the mechanism of the polymer synthesis.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 26281043

Establishment of the next generation microbial factory for lactate-based polymers by utilizing biomass
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2011 - 31 Mar. 2014
TAGUCHI SEIICHI, MATSUMOTO Ken'ichiro, SATOH Toshifumi
We have constructed the microbial system for the lactate-based polymers by using our developed lactate-polymerizing enzyme. The copolymers have been synthesized by achieving the range control of lactate fraction, from 0 to 50 mol% in Escherichia coli and 50 to nearly 100 mol% in Corynebacterium glutamicum. Among them, lactate polymer with 30 mol% lactate fraction was produced up to 11 g/L. In terms of polymer properties, copolymers gained flexibility with increasing lactate fraction compared to the rigid hopolymers such as PLA. Also, microbial polymers were demonstrated to be synthesized by using the enzymatically hydrolyzed sugars from the plant biomass.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23310059

Microbial production of unusual biopolyesters
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)
01 Apr. 2011 - 31 Mar. 2014
MATSUMOTO KENICHIRO
Bacterial polyhydroxyalkanoates (PHAs) are intracellular storage material, which can be used as a biobased plastic. The most common PHA is P(3-hydroxybutyrate), which has been found in a variety of bacteria and produced with good yield. However, the brittleness of the polymer has limited the practical use of the material. This study addressed this problem by using engineered polymer synthetic systems, which are capable synthesizing unusual PHAs. For examples, the isotactic P(2-hydroxybutyrate) was efficiently produced using engineered Escherichia coli. The microbial process had an advantage over the chemical polymerization in that the enzymatic polymerization is capable of synthesizing isotactic polymer from inexpensive racemic precursor. The polyester exhibited excellent transparency and flexibility, which differed from conventional PHAs. The glycolate-based polyester also produced using E. coli. This was the first case for the intracellular polymerization of glycolate.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (A), Hokkaido University, Principal investigator, Competitive research funding, 23681015

Development of microbial factory for bioplastic production adapting inedible biomass hydrolysates
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2011 - 2013
OOI Toshihiko, TAGUCHI Seiichi, KEN'ICHRO Matsumoto
Lignocellulosic biomass is mainly composed of glucose and xylose as the major sugars. The use of glucose for the biopolymer production has been demonstrated to be efficient whereas, the use of xylose is inefficient. However efficient utilization of both sugars is essential. In this study, methods for the efficient utilization of xylose were developed and enhanced to obtain high productivities of biopolymers. For P(LA-co-3HB) production lactic acid supplementation into the cell contributed to the inprovement of polymer yield from xylose. In addition xylose was found to be superiorb to glucose in giving higher LA fraction in the copolymer. The uptake of xylose by GatC which is galactitol transporter also effective to produce the copolymer. The approach for high yield production of biopolymers in combination with the lignocellulosic-derived mix sugars have a potential for the establishment of biorifinary for the production of variety of biopolymers.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 23580452

Production of lactate polymer in the transgenic plants
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
2009 - 2010
MATSUMOTO Kenichiro
Polylactic acid is a biobased polyester, which is synthesized from fermentative lactate. We attempted to synthesize lactate-incorporated polymer using the transgenic plants. To meet this goal, the gene encoding the monomer supplying enzymes and polymerase were introduced into plants. The expression of these genes and polyester synthesis were confirmed.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 21710087

Establishment of the all biosynthetic system for new monomers-contained biopolymers as a basis for enzyme evolutionary engineering
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2008 - 2010
TAGUCHI Seiichi, SATOH Toshifumi, MATSUMOTO Ken'ichiro
Microbial production system for lactate (LA) polymers with high performances has been established. This new microbial factory can be driven by renewable biomass resources. A break-through of this system was a discovery of "LA-Polymerizing Enzyme (LPE)". LPE was created through the study on the engineering of microbial polymer PHA synthase. LA-based polymer was synthesized as an one-pot manner in Escherichia coli by introduction of the LPE gene from glucose. Also, LA fraction in the polymer has been enriched up to 47% from 6% by combination of metabolic and enzyme engineering. Furthermore, LA-based polymers exhibited distinguishable properties from the counterpart homopolymers, PLA and PHB.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 20310050

Analysis of growth inhibition of polyester-producing transgenic plants for efficient polyester production
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
2007 - 2008
MATSUMOTO Kenichiro
バイオプラスチック(PHA)の合成遺伝子を導入した遺伝子組換えイネは、細胞内にPHA を作ることができる。しかしPHA を蓄積することにより、植物の生育が悪くなることが知られていた。
そこで、その原因を解明するため、組換え株と野生株の遺伝子の働きを網羅的に解析し、組換え株の中で遺伝子の働きにどのような変化が起こったのかを調べた。
その結果、組換え株で影響を受けていると考えられる遺伝子を数個見つけることができた。
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 19710076

Analysis of a regulation system by a non-coding RNA that is involved in trans-splicing
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2005 - 2007
SHIMADA Hiroaki, KIKUCHI Shoshi, MATSUMOTO Ken'Ichiro
The gene for a protein kinase, SPK, is divided into two parts. The region for the first part, corresponding to the exon 1, is independently expressed and may function as a non-coding RNA. We found a novel gene encoding an unidentified protein, OsMacl, on the opposite strand of the SPK exon 1 region, suggesting that the exon 1 RNA is a natural antisense RNA and may regulate this protein gene. The mRNA for OsMacl contained a long 5' untranslated sequence and there were several small ORFs, upstream ORF (uORF) , in this region. Since OsMacl was predicted to be localized in the cell membrane, this protein was suggested to be function as somewhat involved in external signals. The reported assay using the GUS gene revealed that the GUS gene downstream the uORFs of OsMacl was efficiently translated. These results suggest that there are some unknown regulation system using such uORFs.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Tokyo University of Science, Coinvestigator not use grants, Competitive research funding, 17310115

生分解性プラスチックを合成する遺伝子組換え植物の創出
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
2004 - 2005
松本 謙一郎
ポリヒドロキシアルカン酸(PHA)は微生物が合成する貯蔵物質であり、生分解性を有するプラスチックとして利用できることから、環境低負荷型のエコ材料として期待されている。当研究課題では、微生物のPHA生合成系遺伝子群を植物に導入することにより、太陽光と二酸化炭素からPHAを直接生産するシステムの開発を目指した。これにより、PHAの実用化への最大の障壁である生産コストが削減できる可能性がある。PHAはモノマー組成に依存して物性が変化することが知られており、炭素数が4(短鎖)のモノマーユニットを主成分とし、少量の炭素数が6以上(中鎖)のモノマーユニットを含む共重合体は、柔軟性がよく幅広い応用範囲が期待できる。しかし、植物細胞内には有効な中鎖のモノマー供給系が存在しないため、共重合体の合成は非常に困難であった。そこで当研究室グループで作成された新規な中鎖モノマー供給酵素である大腸菌由来変異型3-ketoacyl-ACP synthase III (FabH)を用いて、植物の葉緑体内での共重合体の合成を試みた。Pseudomonas sp.61-3及びWautersia eutropha由来のPHA生合成系遺伝子群と変異FabH遺伝子を導入したシロイヌナズナの組換え株は、PHA生合成系遺伝子群のみを導入した組換え株と比較して約2倍のPHAを蓄積した。さらにごく微量ではあるが、炭素数が6〜14のモノマーユニットを含む短鎖・中鎖共重合体が合成された。これより、植物の葉緑体内で中鎖のモノマーを供給する代謝経路の構築に成功した。また、変異型FabHは中鎖よりも短鎖モノマーの供給能力のほうが高いことが分かった。
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), 独立行政法人理化学研究所->東京理科大学, Principal investigator, Competitive research funding, 16710059