Hinou Hiroshi
| Faculty of Advanced Life Science Advanced Transdisciplinary Science Drug Discovery Research | Professor |
Last Updated :2025/12/04
■Researcher basic information
Researchmap personal page
Home Page URL
Researcher ID
- A-4395-2012
J-Global ID
Research Keyword
Research Field
- Life sciences, Bioorganic chemistry
- Nanotechnology/Materials, Synthetic organic chemistry
- Nanotechnology/Materials, Analytical chemistry
- Nanotechnology/Materials, Chemical biology
- Life sciences, Pharmaceuticals - chemistry and drug development
- Life sciences, Bacteriology
- Nanotechnology/Materials, Biochemistry
- Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Applied biofunctional and bioprocess engineering
- Nanotechnology/Materials, Nanobioscience
- Nanotechnology/Materials, Green/sustainable/environmental chemistry
- Environmental science/Agricultural science, Environmental effects of chemicals
Educational Organization
- Bachelor's degree program, School of Science
- Master's degree program, Graduate School of Life Science
- Doctoral (PhD) degree program, Graduate School of Life Science
■Career
Career
- Apr. 2025 - Present
Head, Biological Science (Macromolecular Functions), School of Science, Hokkaido University - Jan. 2019 - Present
Hokkaido University, Faculty of Advanced Life Science, Professor - Apr. 2019 - Mar. 2025
北海道大学 先端生命科学研究院 次世代物質生命科学研究センター副センタ―長 - Apr. 2013 - Dec. 2018
Hokkaido University, Faculty of Advanced Life Science, Associate Professor - Oct. 2007 - Mar. 2013
Hokkaido University, Faculty of Advanced Life Science, Assistant Professor - Feb. 2010 - Sep. 2010
Institute for Research in Biomedicine (IRB barcelona), Visiting researcher - 2006 - 2009
産業技術総合研究所創薬シーズ探索研究ラボ 大学等非常勤研究員 - 2005 - 2007
北海道大学理学研究科 助手 - 2005 - 2006
産業技術総合研究所糖鎖工学研究センター 大学等非常勤研究員 - 2003 - 2005
産業技術総合研究所糖鎖工学研究センター糖鎖自動合成チーム 研究員 - 2002 - 2003
理化学研究所細胞制御化学研究室 共同研究員 - 2002 - 2003
Nihon University, School of Pharmacy - 2000 - 2002
東京理科大学理学部化学科 非常勤講師 - 2000 - 2002
理化学研究所細胞制御化学研究室 協力研究員 - 1999 - 2000
理化学研究所有機合成化学研究室 Junior Research Associate
Educational Background
- Apr. 1997 - Mar. 2000, Saitama University, Graduate School of Science and Engineering, Division of Biological and Environmental Sciences, Japan
- Apr. 1995 - Mar. 1997, Saitama University, Graduate School of Science and Engineering, Division of Applied Chemistry, Japan
- 1995, Saitama University, Faculty of Engineering, 応用化学・環境化学工学科, Japan
Committee Memberships
- Apr. 2021 - Present
日本質量分析学会, 日本質量分析学会北海道談話会・研究会 世話人, Society - Apr. 2021 - Present
高分子学会, 代議員, Society - Apr. 2020 - Present
日本化学会, 北海道支部幹事, Society - Jul. 2019 - Present
日本糖質学会, 評議員 - 2014 - 2023
GFRG, GFRGシンポジウム運営委員, Society - Sep. 2022 - Sep. 2022
高分子学会, 高分子学会高分子討論会実行委員, Society - Sep. 2018 - Sep. 2018
高分子学会, 高分子討論会会場責任者, Society - 2012 - 2014
高分子学会, 「高分子」編集委員会、若手連携委員, Society - Aug. 2012
高分子学会, 高分子若手会・サマーユニバーシティ運営委員, Society - Aug. 2011
高分子学会, 高分子若手会・サマーユニバーシティ運営委員, Society - Jul. 2007 - Jul. 2007
高分子学会, 第５３回 高分子夏季大学実行委員, Society - Feb. 2007
高分子学会, 第41回北海道支部研究発表会実行委員, Society - 2007
高分子学会, 第42回北海道支部研究発表会実行委員, Society - Sep. 2006 - Sep. 2006
繊維学会, 第３７回繊維学会夏季セミナー実行委員, Society
■Research activity information
Awards
- Mar. 2023, John Wiley & Sons, Inc., Top Downloaded Article
Top Downloaded Article
Shogo Urakami;Hiroshi HINOU, 40247315 - Mar. 2022, Analysis & Sensing., Most accessed 03/2022
- 2011, Best Poster Award
- 2011, Very Important Paper (VIP)
- 2007, 高分子研究奨励賞
Japan - 2007, Poster Presentation Award
Papers
- Upregulation of Sulfated N-Glycans in Serum as Predictive Biomarkers for Early-Stage Breast Cancer
Dereje G. Feleke, Bryan M. Montalban, Solomon T. Gizaw, Hiroshi Hinou
International Journal of Molecular Sciences, 22 May 2025, [Peer-reviewed], [Invited], [Last author, Corresponding author]
English, Scientific journal - Characteristic Fragmentation Behavior of Linear and Cyclic O-Linked Glycopeptides and Their Peptide Skeletons in MALDI-TOF/TOF MS
Kohki Fukushi, Shogo Urakami, Hiroshi Hinou
Molecules, 30, 3, 711, 05 Feb. 2025, [Peer-reviewed], [Invited], [Last author, Corresponding author]
English, Scientific journal, 48686282 - MALDI O-antigen glycotyping of Y. pseudotuberculosis using DAN/DHB/K matrix
Shogo Urakami, Hiroshi Hinou
BBA Advances, 7, 100131, 2025, [Peer-reviewed], [Invited], [Last author, Corresponding author]
English, Scientific journal, 48686282 - Integration of MALDI glycotyping and NMR analysis to uncover an O-antigen substructure from pathogenic Escherichia coli O111.
June Chelyn Lee, Shogo Urakami, Hiroshi Hinou
International journal of biological macromolecules, 282, 137178, Elsevier BV, 04 Nov. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Escherichia coli O111 serotype is a critical pathogenic E. coli strain that causes severe, potentially fatal complications. Despite its reported variation, only one structure of the O-antigen polysaccharide from E. coli O111 has been reported. Here, a substructure of the O-antigen from E. coli O111 was characterized using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and NMR analysis. MALDI glycotyping revealed differing O-antigen repeating unit masses of Δm/z 787 and 828 in the E. coli strains and lipopolysaccharides from the O111 serogroup. This variation was caused by the replacement of the hexose residue with hexosamine in the repeating units, which was further confirmed by LIFT-TOF/TOF analysis. Structural elucidation of the O111 substructure by NMR analysis further demonstrated replacement of the hydroxyl group with an N-acetyl group on the terminal glucose residue of the O-antigen pentasaccharide repeating unit. To our knowledge, this study is the first to provide a detailed structural analysis of a new O-antigen substructure from the E. coli O111 serogroup. - MALDI glycotyping of O-antigens from a single colony of gram-negative bacteria.
Shogo Urakami, Hiroshi Hinou
Scientific reports, 14, 1, 12719, 12719, 03 Jun. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Polypeptide-targeted MALDI-TOF MS for microbial species identification has revolutionized microbiology. However, no practical MALDI-TOF MS identification method for O-antigen polysaccharides, a major indicator for epidemiological classification within a species of gram-negative bacteria, is available. We describe a simple MALDI glycotyping method for O-antigens that simultaneously identifies the molecular mass of the repeating units and the monosaccharide composition of the O-antigen. We analyzed the Escherichia coli O1, O6, and O157-type strains. Conventional species identification based on polypeptide patterns and O-antigen polysaccharide typing can be performed in parallel from a single colony using our MALDI-TOF MS workflow. Moreover, subtyping within the same O-antigen and parallel colony-specific O-antigen determination from mixed strains, including the simultaneous identification of multiple strains-derived O-antigens within selected colony, were performed. In MALDI glycotyping of two Enterobacteriaceae strains, a Citrobacter freundii strain serologically cross-reactive with E. coli O157 gave a MALDI spectral pattern identical to E. coli O157. On the other hand, an Edwardsiella tarda strain with no reported O-antigen cross-reactivity gave a MALDI spectral pattern of unknown O-antigen repeating units. The method described in this study allows the parallel and rapid identification of microbial genera, species, and serotypes of surface polysaccharides using a single MALDI-TOF MS instrument. - Sulfated N-glycans Upregulation in Sera Predicts Early-Stage Breast Cancer in Patients
Feleke D. G., Montalban B. M., Gizaw S. T., Hiroshi H.
MedRxiv, 28 Mar. 2024, [Last author, Corresponding author]
Scientific journal - Glycoblotting-Based Ovo-Sulphoglycomics Reveals Phosphorylated N-Glycans as a Possible Host Factor of AIV Prevalence in Waterfowls
Bryan M. Montalban, Hiroshi Hinou
ACS Infectious Diseases, 09 Feb. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 40247315 - Sodium-Doped 3-Amino-4-hydroxybenzoic Acid: Rediscovered Matrix for Direct MALDI Glycotyping of O-Linked Glycopeptides and Intact Mucins
Shogo Urakami, Hiroshi Hinou
International Journal of Molecular Sciences, 28 Nov. 2023, [Peer-reviewed], [Invited], [Last author, Corresponding author]
Scientific journal - DHB Matrix with Additives for Direct MALDI Mass Spectrometry of Carbohydrates and Glycoconjugates
Hiroshi Hinou
Trends in Glycoscience and Glycotechnology, 35, 204, E19, E22, 25 Mar. 2023, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
English, Scientific journal - Altering the Modular Architecture of Galectins Affects its Binding with Synthetic α‐Dystroglycan O‐Mannosylated Core M1 Glycoconjugates In situ
Lareno L. Villones, Anna-Kristin Ludwig, Seiya Kikuchi, Rika Ochi, Shin-Ichiro Nishimura, Hans-Joachim Gabius, Herbert Kaltner, Hiroshi Hinou
ChemBioChem, Wiley, 09 Mar. 2023, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal - Glycoblotting enables seamless and straightforward workflow for MALDI-TOF/MS-based sulphoglycomics of N- and O-glycans
Bryan M. Montalban, Hiroshi Hinou
Proteomics, 2023, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 40247315 - Structural and molecular insight into antibody recognition of dynamic neoepitopes in membrane tethered MUC1 of pancreatic cancer cells and secreted exosomes
Hajime Wakui, Yasuhiro Yokoi, Chieko Horidome, Toyoyuki Ose, Min Yao, Yoshikazu Tanaka, Hiroshi Hinou, Shin-Ichiro Nishimura
RSC Chemical Biology, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed]
Scientific journal, We unveil for the first time that pancreatic cancer cells (PANC-1) and secreted exosomes express MUC1 bearing cancer-relevant dynamic epitopes recognized specifically by an anti-MUC1 antibody (SN-131). - Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples
Shogo Urakami, Hiroshi Hinou
ACS Omega, 7, 43, 39280, 39286, 01 Nov. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 40247315 - Exploring the In situ pairing of human galectins toward synthetic O-mannosylated core M1 glycopeptides of α-dystroglycan
Hiroshi Hinou
Scientific Reports, 12, 1, 23 Oct. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 40247315 - BOA/DHB/Na: An Efficient UV-MALDI Matrix for High-Sensitivity and Auto-Tagging Glycomics
Erina Barada, Hiroshi Hinou
International Journal of Molecular Sciences, 23, 20, 12510, 22 Oct. 2022, [Peer-reviewed], [Invited], [Last author, Corresponding author]
English, Scientific journal, 40247315 - Generation 6 polyamidoamine dendrimer provides an ideal nanoparticular platform for enzyme-assisted synthesis of glycopeptides having bulky and complex glycans
Takahiko Matsushita, Hiroshi Hinou, Shin-Ichiro Nishimura
Chemistry Letters, 51, 1044, 1048, The Chemical Society of Japan, 16 Sep. 2022, [Peer-reviewed]
Scientific journal - Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
Kouta Shiratori, Yasuhiro Yokoi, Hajime Wakui, Nozomi Hirane, Michiru Otaki, Hiroshi Hinou, Tohru Yoneyama, Shingo Hatakeyama, Satoshi Kimura, Chikara Ohyama, Shin-Ichiro Nishimura
RSC advances, 12, 33, 21385, 21393, 21 Jul. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases. The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC. To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers. Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls. Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls. The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling. - Glycan‐Selective MALDI In‐Source Decay Analysis of Intact Glycoproteins
Shogo Urakami, Hiroshi Hinou
Analysis & Sensing, 2, 2, e20210004, Wiley, Mar. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 36102632 - Click Synthesis of Size- and Shape-Tunable Star Polymers with Functional Macrocyclic Cores for Synergistic DNA Complexation and Delivery.
Ana I Carbajo-Gordillo, José L Jiménez Blanco, Juan M Benito, Hugo Lana, Gema Marcelo, Christophe Di Giorgio, Cédric Przybylski, Hiroshi Hinou, Valentín Ceña, Carmen Ortiz Mellet, Francisco Mendicuti, Conchita Tros de Ilarduya, José M García Fernández
Biomacromolecules, 21, 12, 5173, 5188, 14 Dec. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, The architectural perfection and multivalency of dendrimers have made them useful for biodelivery via peripheral functionalization and the adjustment of dendrimer generations. Modulation of the core-forming and internal matrix-forming structures offers virtually unlimited opportunities for further optimization, but only in a few cases this has been made compatible with strict diastereomeric purity over molecularly diverse series, low toxicity, and limited synthetic effort. Fully regular star polymers built on biocompatible macrocyclic platforms, such as hyperbranched cyclodextrins, offer advantages in terms of facile synthesis and flexible compositions, but core elaboration in terms of shape and function becomes problematic. Here we report the synthesis and characterization of star polymers consisting of functional trehalose-based macrocyclic cores (cyclotrehalans, CTs) and aminothiourea dendron arms, which can be efficiently synthesized from sequential click reactions of orthogonal monomers, display no cytotoxicity, and efficiently complex and deliver plasmid DNA in vitro and in vivo. When compared with some commercial cationic dendrimers or polymers, the new CT-scaffolded star polymers show better transfection efficiencies in several cell lines and structure-dependent cell selectivity patterns. Notably, the CT core could be predefined to exert Zn(II) complexing or molecular inclusion capabilities, which has been exploited to synergistically boost cell transfection by orders of magnitude and modulate the organ tropism in vivo. - A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes (vol 11, pg 4999, 2020)
Hajime Wakui, Yoshikazu Tanaka, Toyoyuki Ose, Isamu Matsumoto, Koji Kato, Yao Min, Taro Tachibana, Masaharu Sato, Kentaro Naruchi, Fayna Garcia Martin, Hiroshi Hinou, Shin-Ichiro Nishimura
CHEMICAL SCIENCE, 11, 46, 12588, 12589, Dec. 2020, [Peer-reviewed]
English, Scientific journal - Amplified Detection of Breast Cancer Autoantibodies Using MUC1-Based Tn Antigen Mimics.
Pablo A Guillen-Poza, Elena M Sánchez-Fernández, Gerard Artigas, José Manuel García Fernández, Hiroshi Hinou, Carmen Ortiz Mellet, Shin-Ichiro Nishimura, Fayna Garcia-Martin
Journal of medicinal chemistry, 63, 15, 8524, 8533, 13 Aug. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, In many human carcinomas, mucin-1 (MUC1) is overexpressed and aberrantly glycosylated, resulting in the exposure of previously hidden antigens. This generates new patient antibody profiles that can be used in cancer diagnosis. In the present study, we focused on the MUC1-associated Tn antigen (α-O-GalNAc-Ser/Thr) and substituted the GalNAc monosaccharide by a glycomimic to identify MUC1-based glycopeptides with increased antigenicity. Two different glycopeptide libraries presenting the natural Tn antigen or the sp2-iminosugar analogue were synthesized and evaluated with anti-MUC1 monoclonal antibodies in a microarray platform. The most promising candidates were tested with healthy and breast cancer sera aiming for potential autoantibody-based biomarkers. The suitability of sp2-iminosugar glycopeptides to detect anti-MUC1 antibodies was demonstrated, and serological experiments showed stage I breast cancer autoantibodies binding with a specific unnatural glycopeptide with almost no healthy serum interaction. These results will promote further studies on their capabilities as early cancer biomarkers. - A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes
Hajime Wakui, Yoshikazu Tanaka, Toyoyuki Ose, Isamu Matsumoto, Koji Kato, Yao Min, Taro Tachibana, Masaharu Sato, Kentaro Naruchi, Fayna Garcia Martin, Hiroshi Hinou, Shin-Ichiro Nishimura
CHEMICAL SCIENCE, 11, 19, 4999, 5006, May 2020, [Peer-reviewed]
English, Scientific journal - Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.
Shun Hayakawa, Takahiko Matsushita, Yasuhiro Yokoi, Hajime Wakui, Fayna Garcia-Martin, Hiroshi Hinou, Koji Matsuoka, Kazuhiro Nouso, Toshiya Kamiyama, Akinobu Taketomi, Shin-Ichiro Nishimura
Biochemistry, 59, 12, 1221, 1241, 31 Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy. This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs). NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs. We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region. Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension. An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC). Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients. Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy. - Synthesis, conformational analysis and in vivo assays of an anti-cancer vaccine that features an unnatural antigen based on an sp2-iminosugar fragment
Iris A. Bermejo, Claudio D. Navo, Jorge Castro-López, Ana Guerreiro, Ester Jiménez-Moreno, Elena M. Sánchez Fernández, Fayna García-Martín, Hiroshi Hinou, Shin-Ichiro Nishimura, José M. García Fernández, Carmen Ortiz Mellet, Alberto Avenoza, Jesús H. Busto, Gonçalo J. L. Bernardes, Ramón Hurtado-Guerrero, Jesús M. Peregrina, Francisco Corzana
Chemical Science, 11, 15, 3996, 4006, Royal Society of Chemistry (RSC), 2020, [Peer-reviewed]
English, Scientific journal,An anti-cancer vaccine based on an unnatural antigen with an sp2-iminosugar fragment.
- Aniline derivative/DHB/alkali metal matrices for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides
Hiroshi Hinou
International Journal of Mass Spectrometry, Sep. 2019, [Peer-reviewed], [Lead author, Last author, Corresponding author]
English, Scientific journal - Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles.
Sho Hideshima, Hiroki Hayashi, Hiroshi Hinou, Shunsuke Nambuya, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Shin-Ichiro Nishimura, Yoshihiro Sakoda, Tetsuya Osaka
Scientific reports, 9, 1, 11616, 11616, 12 Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Pandemic influenza, triggered by the mutation of a highly pathogenic avian influenza virus (IFV), has caused considerable damage to public health. In order to identify such pandemic IFVs, antibodies that specifically recognize viral surface proteins have been widely used. However, since the analysis of a newly discovered virus is time consuming, this delays the availability of suitable detection antibodies, making this approach unsuitable for the early identification of pandemic IFVs. Here we propose a label-free semiconductor-based biosensor functionalized with sialic-acid-containing glycans for the rapid identification of the pandemic IFVs present in biological fluids. Specific glycans are able to recognize wild-type human and avian IFVs, suggesting that they are useful in discovering pandemic IFVs at the early stages of an outbreak. We successfully demonstrated that a dual-channel integrated FET biosensing system, which were modified with 6'-sialyllactose and 3'-sialyllactose for each gate area, can directly and specifically detect human H1N1 and avian H5N1 IFV particles, respectively, present in nasal mucus. Furthermore, to examine the possibility of identifying pandemic IFVs, the signal attributed to the detection of Newcastle disease virus (NDV) particles, which was selected as a prime model of a pandemic IFV, was clearly observed from both sensing gates. Our findings suggest that the proposed glycan-immobilized sensing system could be useful in identifying new pandemic IFVs at the source of an outbreak. - Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.
Hiroshi Hinou
BMC cancer, 19, 1, 588, 17 Jun. 2019, [Peer-reviewed]
Scientific journal - Synthetic glycopeptides reveal specific binding pattern and conformational change at O-mannosylated position of α-dystroglycan by POMGnT1 catalyzed GlcNAc modification.
Hiroshi Hinou
Bioorganic & medicinal chemistry, 27, 13, 2822, 2831, 06 May 2019, [Peer-reviewed], [Lead author, Corresponding author]
Scientific journal - Novel matrix system for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides
Hiroshi Hinou
Abstracts of Papers of the American Chemical Society, 2019
Scientific journal - Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species
Jurgen T. Sanes, Hiroshi Hinou, Yuan Chuan Lee, Shin-Ichiro Nishimura
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 67, 1, 531, 540, Jan. 2019, [Peer-reviewed]
English, Scientific journal - Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.
Hiroshi Hinou
PloS one, 13, 12, e0209515, 28 Dec. 2018, [Peer-reviewed]
Scientific journal - Synthetic Glycopeptides Allow for the Quantitation of Scarce Nonfucosylated IgG Fc N-Glycans of Therapeutic Antibody
Hiroshi Hinou
ACS Medicinal Chemistry Letters, 9, 9, 889, 894, Sep. 2018, [Peer-reviewed]
Scientific journal - Synthetic glycopeptides as a designated standard in focused glycoproteomics to discover serum cancer biomarkers
K. Yogesh, Toshiya Kamiyama, Chikara Ohyama, Tohru Yoneyama, Kazuhiro Nouso, Satoshi Kimura, Hiroshi Hinou, Shin-Ichiro Nishimura
MEDCHEMCOMM, 9, 8, 1351, 1358, Aug. 2018, [Peer-reviewed]
English, Scientific journal - Further applications of classical amide coupling reagents: Microwave-assisted esterification on solid phase
Risa Takayama, Shun Hayakawa, Hiroshi Hinou, Fernando Albericio, Fayna Garcia-Martin
JOURNAL OF PEPTIDE SCIENCE, 24, 8-9, e3111, Aug. 2018, [Peer-reviewed]
English, Scientific journal - The Use of Fluoroproline in MUC1 Antigen Enables Efficient Detection of Antibodies in Patients with Prostate Cancer
Victor J. Somovilla, Iris A. Bermejo, Ines S. Albuquerque, Nuria Martinez-Saez, Jorge Castro-Lopez, Fayna Garcia-Martin, Ismael Companon, Hiroshi Hinou, Shin-Ichiro Nishimura, Jesus Jimenez-Barbero, Juan L. Asensio, Alberto Avenoza, Jesus H. Busto, Ramon Hurtado-Guerrero, Jesus M. Peregrina, Goncalo J. L. Bernardes, Francisco Corzana
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 139, 50, 18255, 18261, Dec. 2017, [Peer-reviewed]
English, Scientific journal - Glycopeptides as Targets for Dendritic Cells: Exploring MUC1 Glycopeptides Binding Profile toward Macrophage Galactose-Type Lectin (MGL) Orthologs
Gerard Artigas, Joao T. Monteiro, Hiroshi Hinou, Shin-Ichiro Nishimura, Bernd Lepenies, Fayna Garcia-Martin
JOURNAL OF MEDICINAL CHEMISTRY, 60, 21, 9012, 9021, Nov. 2017, [Peer-reviewed]
English, Scientific journal - An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein
Takayuki Furukawa, Hiroshi Hinou, Seiji Takeda, Hitoshi Chiba, Shin-Ichiro Nishimura, Shu-Ping Hui
CHEMBIOCHEM, 18, 19, 1903, 1909, Oct. 2017, [Peer-reviewed]
English, Scientific journal - Chemical Synthesis Demonstrates That Dynamic O-Glycosylation Regulates the Folding and Functional Conformation of a Pivotal EGF12 Domain of the Human NOTCH1 Receptor
Shun Hayakawa, Yasuhiro Yokoi, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 56, 33, 4379, 4391, Aug. 2017, [Peer-reviewed]
English, Scientific journal - Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays
Gerard Artigas, Hiroshi Hinou, Fayna Garcia-Martin, Hans-Joachim Gabius, Shin-Ichiro Nishimura
CHEMISTRY-AN ASIAN JOURNAL, 12, 1, 159, 167, Jan. 2017, [Peer-reviewed], [Corresponding author]
English, Scientific journal - Glycoblotting method allows for rapid and efficient glycome profiling of human Alzheimer's disease brain, serum and cerebrospinal fluid towards potential biomarker discovery
Solomon T. Gizaw, Tetsu Ohashi, Masakazu Tanaka, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1860, 8, 1716, 1727, Aug. 2016, [Peer-reviewed]
English, Scientific journal - Convergent Solid-Phase Synthesis of Macromolecular MUC1 Models Truly Mimicking Serum Glycoprotein Biomarkers of Interstitial Lung Diseases
Naoki Ohyabu, Kiyoshi Kakiya, Yasuhiro Yokoi, Hiroshi Hinou, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138, 27, 8392, 8395, Jul. 2016, [Peer-reviewed]
English, Scientific journal - Solid-phase synthesis of C-mannosylated glycopeptide on WSXWS motif of human erythropoietin receptor
Hiroshi Hinou, Yuya Abe, Shun Hayakawa, Kentaro Naruchi, Naoki Fujitani, Shin-Ichiro Nishimura
TETRAHEDRON LETTERS, 57, 7, 791, 795, Feb. 2016, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Synthetic Human NOTCH1 EGF Modules Unraveled Molecular Mechanisms for the Structural and Functional Roles of Calcium Ions and O-Glycans in the Ligand-Binding Region
Shun Hayakawa, Ryosuke Koide, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 55, 5, 776, 787, Feb. 2016, [Peer-reviewed]
English, Scientific journal - Novel beta-galactosynthase-beta-mannosynthase dual activity of beta-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates
R. S. Tan, H. Hinou, S. -I. Nishimura
RSC ADVANCES, 6, 56, 50833, 50836, 2016, [Peer-reviewed]
English, Scientific journal - Effects of the multiple O-glycosylation states on antibody recognition of the immunodominant motif in MUC1 extracellular tandem repeats
Shobith Rangappa, Gerard Artigas, Risho Miyoshi, Yasuhiro Yokoi, Shun Hayakawa, Fayna Garcia-Martin, Hiroshi Hinou, Shin-Ichiro Nishimura
MEDCHEMCOMM, 7, 6, 1102, 1122, 2016, [Peer-reviewed]
English, Scientific journal - Large-Scale Glycomics of Livestock: Discovery of Highly Sensitive Serum Biomarkers Indicating an Environmental Stress Affecting Immune Responses and Productivity of Holstein Dairy Cows
Ibrahim F. Rehan, Koichiro Ueda, Tomohiro Mitani, Maho Amano, Hiroshi Hinou, Tetsu Ohashi, Seiji Kondo, Shin-Ichiro Nishimura
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 63, 48, 10578, 10590, Dec. 2015, [Peer-reviewed]
English, Scientific journal - The Quest for Anticancer Vaccines: Deciphering the Fine-Epitope Specificity of Cancer-Related Monoclonal Antibodies by Combining Microarray Screening and Saturation Transfer Difference NMR
Helena Coelho, Talcahiko Matsushita, Gerard Artigas, Hiroshi Hinou, F. Javier Canada, Richard Lo-Man, Claude Leclerc, Eurico J. Cabrita, Jesus Jimenez-Barbero, Shin-Ichiro Nishimura, Fayna Garcia-Martin, Filipa Marcelo
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 137, 39, 12438, 12441, Oct. 2015, [Peer-reviewed]
English, Scientific journal - A comprehensive glycome profiling of Huntington's disease transgenic mice
Solomon T. Gizaw, Toshiaki Koda, Maho Amano, Keiko Kamimura, Tetsu Ohashi, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1850, 9, 1704, 1718, Sep. 2015, [Peer-reviewed]
English, Scientific journal - Rapid Endolysosomal Escape and Controlled Intracellular Trafficking of Cell Surface Mimetic Quantum-Dots-Anchored Peptides and Glycopeptides
Roger S. Tan, Kentaro Naruchi, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura
ACS CHEMICAL BIOLOGY, 10, 9, 2073, 2086, Sep. 2015, [Peer-reviewed]
English, Scientific journal - A comprehensive glycome profiling of Huntington's disease transgenic mice.
Gizaw ST, Koda T, Amano M, Kamimura K, Ohashi T, Hinou H, Nishimura SI
Biochimica et biophysica acta, 1850, 9, 1704, 1718, Apr. 2015, [Peer-reviewed] - Fast Epitope Mapping for the Anti-MUC1 Monoclonal Antibody by Combining a One-Bead-One-Glycopeptide Library and a Microarray Platform
Fayna Garcia-Martin, Takahiko Matsushita, Hiroshi Hinou, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 20, 48, 15891, 15902, Nov. 2014, [Peer-reviewed]
English, Scientific journal - A straightforward protocol for the preparation of high performance microarray displaying synthetic MUC1 glycopeptides.
Matsushita T, Takada W, Igarashi K, Naruchi K, Miyoshi R, Garcia-Martin F, Amano M, Hinou H, Nishimura S
Biochimica et biophysica acta, 1840, 3, 1105, 1116, 3, Mar. 2014, [Peer-reviewed]
Scientific journal - A straightforward protocol for the preparation of high performance microarray displaying synthetic MUC1 glycopeptides
Takahiko Matsushita, Wataru Takada, Kota Igarashi, Kentaro Naruchi, Risho Miyoshi, Fayna Garcia-Martin, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1840, 3, 1105, 1116, Mar. 2014, [Peer-reviewed]
English, Scientific journal - Synthesis of neoglycosphingolipid from methoxyamino-functionalized ceramide
Junya Ishida, Hiroshi Hinou, Kentaro Naruchi, Shin-Ichiro Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 24, 4, 1197, 1200, Feb. 2014, [Peer-reviewed]
English, Scientific journal - Potential usage for in vivo lectin screening in live animals utilizing cell surface mimetic glyco-nanoparticles, phosphorylcholine-coated quantum dots (PC-QDs)
Maho Amano, Hiroshi Hinou, Risho Miyoshi, Shin-Ichiro Nishimura
Methods in Molecular Biology, 1200, 361, 369, Humana Press Inc., 2014, [Peer-reviewed], [Invited]
English, Scientific journal - 糖タンパク質の糖鎖を付け替える 均一な糖鎖構造をもつ糖タンパク質の効率的合成
HINO HIROSHI, NISHIMURA SHIN'ICHIRO
化学, 69, 1, 74, 75, 2014, [Invited], [Lead author, Corresponding author]
Japanese - A New Approach for the Synthesis of Hyperbranched N-Glycan Core Structures from Locust Bean Gum
Ravi H. Kumar, Kentaro Naruchi, Risho Miyoshi, Hiroshi Hinou, Shin-Ichiro Nishimura
ORGANIC LETTERS, 15, 24, 6278, 6281, Dec. 2013, [Peer-reviewed]
English, Scientific journal - A novel approach for the parallel synthesis of glycopeptides by combining solid-phase peptide synthesis and dendrimer-supported enzymatic modifications
Takahiko Matsushita, Seiji Handa, Kentaro Naruchi, Fayna Garcia-Martin, Hiroshi Hinou, Shin-Ichiro Nishimura
Polymer Journal, 45, 8, 854, 862, Aug. 2013, [Invited]
English, Scientific journal - Attomolar Detection of Influenza A Virus Hemagglutinin Human H1 and Avian H5 Using Glycan-Blotted Field Effect Transistor Biosensor
Sho Hideshima, Hiroshi Hinou, Daisuke Ebihara, Ryosuke Sato, Shigeki Kuroiwa, Takuya Nakanishi, Shin-Ichiro Nishimura, Tetsuya Osaka
ANALYTICAL CHEMISTRY, 85, 12, 5641, 5644, Jun. 2013, [Peer-reviewed]
English, Scientific journal - Microwave-Assisted Solid-Phase Synthesis of Antifreeze Glycopeptides
Ryukou Izumi, Takahiko Matsushita, Naoki Fujitani, Kentaro Naruchi, Hiroki Shimizu, Sakae Tsuda, Hiroshi Hinou, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 19, 12, 3913, 3920, Mar. 2013, [Peer-reviewed]
English, Scientific journal - Glycoblotting-based high throughput protocol for the structural characterization of hyaluronan degradation products during enzymatic fragmentation
Takayuki Furukawa, Misaki Arai, Fayna Garcia-Martin, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura
GLYCOCONJUGATE JOURNAL, 30, 2, 171, 182, Feb. 2013, [Peer-reviewed]
English, Scientific journal - Macrocyclic Mechanism-Based Inhibitor for Neuraminidases
Hirokazu Kai, Hiroshi Hinou, Kentaro Naruchi, Takahiko Matsushita, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 19, 4, 1364, 1372, Jan. 2013, [Peer-reviewed], [Corresponding author]
English, Scientific journal - Site-Specific Conformational Alteration Induced by Sialylation of MUC1 Tandem Repeating Glycopeptides at an Epitope Region for the Anti-KL-6 Monoclonal Antibody
Takahiko Matsushita, Naoki Ohyabu, Naoki Fujitani, Kentaro Naruchi, Hiroki Shimizu, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 52, 2, 402, 414, Jan. 2013, [Peer-reviewed]
English, Scientific journal - Molecular shuttle between extracellular and cytoplasmic space allows for monitoring of GAG biosynthesis in human articular chondrocytes
Hiroshi Hinou
Biochimica et Biophysica Acta (BBA) - General Subjects, 1820, 9, 1391, 1398, Sep. 2012, [Peer-reviewed]
Scientific journal - Aglycone-focused randomization of 2-difluoromethylphenyl-type sialoside suicide substrates for neuraminidases
Hirokazu Kai, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY, 20, 8, 2739, 2746, Apr. 2012, [Peer-reviewed], [Corresponding author]
English, Scientific journal - Strict Stereocontrol by 2,4-O-Di-tert-butylsilylene Group on beta-Glucuronylations
Takayuki Furukawa, Hiroshi Hinou, Shin-Ichiro Nishimura
ORGANIC LETTERS, 14, 8, 2102, 2105, Apr. 2012, [Peer-reviewed], [Corresponding author]
English, Scientific journal - An efficient protocol for the solid-phase synthesis of glycopeptides under microwave irradiation
Fayna Garcia-Martin, Hiroshi Hinou, Takahiko Matsushita, Shun Hayakawa, Shin-Ichiro Nishimura
ORGANIC & BIOMOLECULAR CHEMISTRY, 10, 8, 1612, 1617, 2012, [Peer-reviewed]
English, Scientific journal - H-bonding promotion of peptide solubility and cyclization by fluorinated alcohols
Hiroshi Hinou, Kei Hyugaji, Fayna Garcia-Martin, Shin-Ichiro Nishimura, Fernando Albericio
RSC ADVANCES, 2, 7, 2729, 2731, 2012, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - A potential glucuronate glycosyl donor with 2-O-acyl-6,3-lactone structure: efficient synthesis of glycosaminoglycan disaccharides
Takayuki Furukawa, Hiroshi Hinou, Ken Shimawaki, Shin-Ichiro Nishimura
TETRAHEDRON LETTERS, 52, 43, 5567, 5570, Oct. 2011, [Peer-reviewed], [Corresponding author]
English, Scientific journal - Importance of Sialic Acid Residues Illuminated by Live Animal Imaging Using Phosphorylcholine Self-Assembled Monolayer-Coated Quantum Dots
Tatsuya Ohyanagi, Noriko Nagahori, Ken Shimawaki, Hiroshi Hinou, Tadashi Yamashita, Akira Sasaki, Takashi Jin, Toshihiko Iwanaga, Masataka Kinjo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133, 32, 12507, 12517, Aug. 2011, [Peer-reviewed]
English, Scientific journal - A Strategy for Neuraminidase Inhibitors Using Mechanism-Based Labeling Information
Hiroshi Hinou, Risho Miyoshi, Yasuaki Takasu, Hirokazu Kai, Masaki Kurogochi, Shingo Arioka, Xiao-Dong Gao, Nobuaki Miura, Naoki Fujitani, Shinya Omoto, Tomokazu Yoshinaga, Tamio Fujiwara, Takeshi Noshi, Hiroko Togame, Hiroshi Takemoto, Shin-Ichiro Nishimura
CHEMISTRY-AN ASIAN JOURNAL, 6, 4, 1048, 1056, Apr. 2011, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - An Efficient Approach for the Characterization of Mucin-Type Glycopeptides: The Effect of O-Glycosylation on the Conformation of Synthetic Mucin Peptides
Ryo Hashimoto, Naoki Fujitani, Yasuhiro Takegawa, Masaki Kurogochi, Takahiko Matsushita, Kentaro Naruchi, Naoki Ohyabu, Hiroshi Hinou, Xiao Dong Gao, Naomi Manri, Hiroyuki Satake, Akihito Kaneko, Takeshi Sakamoto, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 17, 8, 2393, 2404, Feb. 2011, [Peer-reviewed]
English, Scientific journal - Toward Green and Sustainable Chemical Glycosylation: Enhanced Lewis Acidity of Recyclable Solid Super Acid Catalyst, SO4/ZrO2 by CaCl2 Doping
Hiroshi Hinou, Naohiro Saito, Takahiro Maeda, Masao Matsuda, Yuichi Kamiya, Shin-Ichiro Nishimura
JOURNAL OF CARBOHYDRATE CHEMISTRY, 30, 7-9, 575, 586, 2011, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Glycoblotting-Assisted O-Glycomics: Ammonium Carbamate Allows for Highly Efficient O-Glycan Release from Glycoproteins
Yoshiaki Miura, Kentaro Kato, Yasuhiro Takegawa, Masaki Kurogochi, Jun-ichi Furukawa, Yasuro Shinohara, Noriko Nagahori, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura
ANALYTICAL CHEMISTRY, 82, 24, 10021, 10029, Dec. 2010, [Peer-reviewed]
English, Scientific journal - Artificial Golgi Apparatus: Globular Protein-like Dendrimer Facilitates Fully Automated Enzymatic Glycan Synthesis
Takahiko Matsushita, Izuru Nagashima, Masataka Fumoto, Takashi Ohta, Kuriko Yamada, Hiroki Shimizu, Hiroshi Hinou, Kentaro Naruchi, Takaomi Ito, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132, 46, 16651, 16656, Nov. 2010, [Peer-reviewed]
English, Scientific journal - Chemical Synthesis, Folding, and Structural Insights into O-Fucosylated Epidermal Growth Factor-like Repeat 12 of Mouse Notch-1 Receptor
Kazumi Hiruma-Shimizu, Kensaku Hosoguchi, Yan Liu, Naoki Fujitani, Takashi Ohta, Hiroshi Hinou, Takahiko Matsushita, Hiroki Shimizu, Ten Feizo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132, 42, 14857, 14865, Oct. 2010, [Peer-reviewed]
English, Scientific journal - A new class of mechanism-based inhibitors for Trypanosoma cruzi trans-sialidase and their influence on parasite virulence
Sebastiao T. Carvalho, Mauro Sola-Penna, Isadora A. Oliveira, Samuel Pita, Arlan S. Goncalves, Bianca C. Neves, Francisco R. Sousa, Leonardo Freire-de-Lima, Masaki Kurogochi, Hiroshi Hinou, Shin-Ichiro Nishimura, Lucia Mendonca-Previato, Jose O. Previato, Adriane R. Todeschini
GLYCOBIOLOGY, 20, 8, 1034, 1045, Aug. 2010, [Peer-reviewed]
English, Scientific journal - An Efficient Approach to the Discovery of Potent Inhibitors against Glycosyltransferases
Kensaku Hosoguchi, Takahiro Maeda, Jun-ichi Furukawa, Yasuro Shinohara, Hiroshi Hinou, Mitsuaki Sekiguchi, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF MEDICINAL CHEMISTRY, 53, 15, 5607, 5619, Aug. 2010, [Peer-reviewed]
English, Scientific journal - Unexpected Tolerance of Glycosylation by UDP-GalNAc:Polypeptide alpha-N-Acetylgalactosaminyltransferase Revealed by Electron Capture Dissociation Mass Spectrometry: Carbohydrate as Potential Protective Groups
Yayoi Yoshimura, Takahiko Matsushita, Naoki Fujitani, Yasuhiro Takegawa, Haruhiko Fujihira, Kentarou Naruchi, Xiao-Dong Gao, Naomi Manri, Takeshi Sakamoto, Kentaro Kato, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 49, 28, 5929, 5941, Jul. 2010, [Peer-reviewed]
English, Scientific journal - Potent inhibitor scaffold against Trypanosoma cruzi trans-sialidase
Shingo Arioka, Masahiro Sakagami, Rie Uematsu, Hiroto Yamaguchi, Hiroko Togame, Hiroshi Takemoto, Hiroshi Hinou, Shin-ichiro Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY, 18, 4, 1633, 1640, Feb. 2010, [Peer-reviewed]
English, Scientific journal - An Efficient Strategy for the Exploration of Specific Inhibitors of Sialyltransferases
Hiroshi Hinou
Molecular Imaging for Integrated Medical Therapy and Drug Development, 2010
Scientific journal - An Essential Epitope of Anti-MUC1 Monoclonal Antibody KL-6 Revealed by Focused Glycopeptide Library
Naoki Ohyabu, Hiroshi Hinou, Takahiko Matsushita, Ryukou Izumi, Hiroki Shimizu, Keiko Kawamoto, Yoshito Numata, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131, 47, 17102, 17109, Dec. 2009, [Peer-reviewed]
English, Scientific journal - Microwave Effect for Glycosylation Promoted by Solid Super Acid in Supercritical Carbon Dioxide
Hiroshi Hinou, Naohiro Saito, Masato Ogawa, Takahiko Maeda, Shin-Ichiro Nishimura
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 10, 12, 5285, 5295, Dec. 2009, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
English, Scientific journal - Functional Neoglycopeptides: Synthesis and Characterization of a New Class of MUC1 Glycoprotein Models Having Core 2-Based O-Glycan and Complex-Type N-Glycan Chains
Takahiko Matsushita, Reiko Sadamoto, Naoki Ohyabu, Hideki Nakata, Masataka Fumoto, Naoki Fujitani, Yasuhiro Takegawa, Takeshi Sakamoto, Masaki Kurogochi, Hiroshi Hinou, Hiroki Shimizu, Takaomi Ito, Kentarou Naruchi, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura
BIOCHEMISTRY, 48, 46, 11117, 11133, Nov. 2009, [Peer-reviewed]
English, Scientific journal - One-pot synthesis of cyclic antifreeze glycopeptides
Masakazu Hachisu, Hiroshi Hinou, Manabu Takamichi, Sakae Tsuda, Shuhei Koshidaa, Shin-Ichiro Nishimura
CHEMICAL COMMUNICATIONS, 13, 1641, 1643, 2009, [Peer-reviewed]
English, Scientific journal - An Efficient Strategy for the Explortion of Specific Inhibitors of Sialyltransferases
Kensku Hosoghchi, Hiroshi Hinou, Shin-Ichiro Nishimura
Proceeding of Molecular Imaging for Integrated Medical Therapy and Drug Development, 294, 301, 2009, [Invited]
English - A new glycosylation method part 3: study of microwave effects at low temperatures to control reaction pathways and reduce byproducts
Hiroki Shimizu, Yayoyi Yoshimura, Hiroshi Hinou, Shin-Ichiro Nishimura
TETRAHEDRON, 64, 43, 10091, 10096, Oct. 2008, [Peer-reviewed]
English, Scientific journal - One-pot solid-phase glycoblotting and probing by transoximization for high-throughput glycomics and glycoproteomics
Hideyuki Shimaoka, Hiromitsu Kuramoto, Jun-ichi Furukawa, Yoshiaki Miura, Masaki Kurogochi, Yoko Kita, Hiroshi Hinou, Yasuro Shinohara, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 13, 6, 1664, 1673, 2007, [Peer-reviewed]
English, Scientific journal - Structural analysis of O-glycopeptides employing negative- and positive-ion multi-stage mass spectra obtained by collision-induced and electron-capture dissociations in linear ion trap time-of-flight mass spectrometry
Kisaburo Deguchi, Hiroki Ito, Takashi Baba, Atsumu Hirabayashi, Hiroaki Nakagawa, Masataka Fumoto, Hiroshi Hinou, Shin-Ichiro Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21, 5, 691, 698, 2007, [Peer-reviewed]
English, Scientific journal - Highly efficient and versatile synthesis of proteoglycan core structures from 1,6-anhydro-beta-lactose as a key starting material
Ken Shimawaki, Yoshinori Fujisawa, Fumihiro Sato, Naoki Fujitani, Masaki Kurogochi, Hiroko Hoshi, Hiroshi Hinou, Shin-Ichiro Nishiinura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 46, 17, 3074, 3079, 2007, [Peer-reviewed]
English, Scientific journal - Construction and structural characterization of versatile lactosaminoglycan-related compound library for the synthesis of complex glycopeptides and glycosphingolipids
Kentarou Naruchi, Tomoki Hamamoto, Masaki Kurogochi, Hiroshi Hinou, Hiroki Shimizu, Takahiko Matsushita, Naoki Fujitani, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF ORGANIC CHEMISTRY, 71, 26, 9609, 9621, Dec. 2006, [Peer-reviewed]
English, Scientific journal - Construction of MUC1 related compound library
Naoki Ohyabu, Takahiko Matsushita, Hiroshi Hinou, Ryuko Izumi, Hiroki Shimizu, Hirosato Kondo, Shin-Ichiro Nishimura
GLYCOBIOLOGY, 16, 11, 1152, 1152, Nov. 2006, [Peer-reviewed]
English - Structural analysis of O-glycopeptides employing negative- and positive-ion MSn spectra acquired by nanoHPLC/ESI-linear ion trap time-of-flight mass spectrometer
Hiroki Ito, Kisaburo Deguchi, Kuriko Yamada, Shinji Nagai, Masataka Fumoto, Hiroshi Hinou, Hiroaki Nakagawa, Yasuro Shinohara, Shin-Ichiro Nishimura
GLYCOBIOLOGY, 16, 11, 1136, 1136, Nov. 2006, [Peer-reviewed]
English - Construction of highly glycosylated mucin-type glycopeptides based on microwave-assisted solid-phase syntheses and enzymatic modifications
T Matsushita, H Hinou, M Fumoto, M Kurogochi, N Fujitani, H Shimizu, SI Nishimura
JOURNAL OF ORGANIC CHEMISTRY, 71, 8, 3051, 3063, Apr. 2006, [Peer-reviewed]
English, Scientific journal - Glycosidation promoted by a reusable solid superacid in supercritical carbon dioxide
Xue-Bing Li, Masato Ogawa, Toshiki Monden, Takahiro Maeda, Eri Yamashita, Mariko Naka, Masao Matsuda, Hiroshi Hinou, Shin-Ichiro Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 45, 34, 5652, 5655, 2006, [Peer-reviewed]
English, Scientific journal - Mechanism-based inhibitors to probe transitional states of glycoside hydrolases
Hiroshi Hinou, Masaki Kurogochi, Shin-Ichiro Nishimura
GLYCOBIOLOGY, 415, 202, +, 2006, [Peer-reviewed]
English, In book - Characterization of Vibrio cholerae neuraminidase by a novel mechanism-based fluorescent labeling reagent
H Hinou, M Kurogochi, H Shimizu, SI Nishimura
BIOCHEMISTRY, 44, 35, 11669, 11675, Sep. 2005, [Peer-reviewed], [Lead author]
English, Scientific journal - Combinatorial synthesis of MUC1 glycopeptides: Polymer blotting facilitates chemical and enzymatic synthesis of highly complicated mucin glycopeptides
M Fumoto, H Hinou, T Ohta, T Ito, K Yamada, A Takimoto, H Kondo, H Shimizu, T Inazu, Y Nakahara, SI Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 127, 33, 11804, 11818, Aug. 2005, [Peer-reviewed]
English, Scientific journal - A novel glycosylation concept; microwave-assisted acetal-exchange type glycosylations from methyl glycosides as donors
Y Yoshimura, H Shimizu, H Hinou, SI Nishimura
TETRAHEDRON LETTERS, 46, 28, 4701, 4705, Jul. 2005, [Peer-reviewed]
English, Scientific journal - Rapid microwave-assisted solid-phase glycopeptide synthesis
T Matsushita, H Hinou, M Kurogochi, H Shimizu, SI Nishimura
ORGANIC LETTERS, 7, 5, 877, 880, Mar. 2005, [Peer-reviewed]
English, Scientific journal - High-throughput protein glycomics: Combined use of chemoselective glycoblotting and MALDI-TOF/TOF mass spectrometry
SI Nishimura, K Niikura, M Kurogochi, T Matsushita, M Fumoto, H Hinou, R Kamitani, H Nakagawa, K Deguchi, N Miura, K Monde, H Kondo
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 44, 1, 91, 96, 2005, [Peer-reviewed]
English, Scientific journal - Molecular transporter between polymer platforms: Highly efficient chemoenzymatic glycopeptide synthesis by the combined use of solid-phase and water-soluble polymer supports
M Fumoto, H Hinou, T Matsushita, M Kurogochi, T Ohta, T Ito, K Yamada, A Takimoto, H Kondo, T Inazu, SI Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 44, 17, 2534, 2537, 2005, [Peer-reviewed]
English, Scientific journal - Systematic syntheses and inhibitory activities of bisubstrate-type inhibitors of sialyltransferases
H Hinou, XL Sun, Y Ito
JOURNAL OF ORGANIC CHEMISTRY, 68, 14, 5602, 5613, Jul. 2003, [Peer-reviewed], [Lead author]
English, Scientific journal - Efficient strategy for convergent synthesis of trans-fused polycyclic ethers based on an intramolecular SmI2-promoted cyclization of iodo ester
K Kawamura, H Hinou, G Matsuo, T Nakata
TETRAHEDRON LETTERS, 44, 28, 5259, 5261, Jul. 2003, [Peer-reviewed]
English, Scientific journal - Glyco-silicon functional materials. Part 6. Synthesis of a useful anomeric thioacetate of an N-acetyllactosamine derivative and its application
K Matsuoka, T Ohtawa, H Hinou, T Koyama, Y Esumi, SI Nishimura, K Hatano, D Terunuma
TETRAHEDRON LETTERS, 44, 18, 3617, 3620, Apr. 2003, [Peer-reviewed]
English, Scientific journal - Bisubstrate-type inhibitor of sialyltransferases
H Hinou, XL Sun, Y Ito
TETRAHEDRON LETTERS, 43, 50, 9147, 9150, Dec. 2002, [Peer-reviewed], [Lead author]
English, Scientific journal - Disaccharides as starting materials for the synthesis of valuable compounds
H Hinou
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, 12, 65, 185, 190, May 2000, [Invited], [Lead author, Last author, Corresponding author]
English, Scientific journal - Efficient convergent synthesis of a trans-fused 6-6-6-6-membered tetracyclic ether ring system
G Matsuo, H Hinou, H Koshino, T Suenaga, T Nakata
TETRAHEDRON LETTERS, 41, 6, 903, 906, Feb. 2000, [Peer-reviewed]
English, Scientific journal - Synthesis of amphiphilic chitopentaose and chitoheptaose derivatives using a common disaccharidic synthon as the chain elongation unit
H Hinou, A Umino, K Matsuoka, D Terunuma, S Takahashi, Y Esumi, H Kuzuhara
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 73, 1, 163, 171, Jan. 2000, [Peer-reviewed], [Lead author]
English, Scientific journal - Novel synthesis of L-iduronic acid using trehalose as the disaccharidic starting material
Hiroshi Hinou, Hidehiro Kurosawa, Koji Matsuoka, Daiyo Terunuma, Hiroyoshi Kuzuhara
Tetrahedron Letters, 40, 8, 1501, 1504, 19 Feb. 1999, [Peer-reviewed], [Lead author]
English, Scientific journal - Synthetic conversion of cellobiose into the glycal-type monomers and their polymerization
Y Ogawa, H Hinou, K Matsuoka, D Terunuma, H Kuzuhara
TETRAHEDRON LETTERS, 39, 32, 5789, 5792, Aug. 1998, [Peer-reviewed]
English, Scientific journal - Efficient conversion of a 1,6-anhydro chitobiose derivative into the corresponding tetradecyl beta-glycoside derivative by means of participation of a neighboring tetradecanamide group
A Umino, H Hinou, K Matsuoka, D Terunuma, S Takahashi, H Kuzuhara
JOURNAL OF CARBOHYDRATE CHEMISTRY, 17, 2, 231, 239, 1998, [Peer-reviewed]
English, Scientific journal - Synthesis of Amphiphilic Chitoheptaose Derivative
Hiroshi Hinou, Atsushi Umino, Koji Matsuoka, Daiyo Terunuma, Shunya Takahashi, Yasuaki Esumi, Hiroyoshi Kuzuhara
Tetrahedron Letters, 38, 46, 8041, 8044, Elsevier BV, Nov. 1997, [Peer-reviewed], [Lead author]
English, Scientific journal
Other Activities and Achievements
- How to Analyze the Diversity of Glycans: Functional Glycotyping and Structural Glycotyping
比能洋, 化学と生物, 62, 8, Aug. 2024, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
Japanese, Introduction scientific journal - Y. pseudotuberculosis O-antigen rapid typing by MALDI-MS
浦上彰吾, 比能洋, 日本糖質学会年会要旨集, 43rd, 2024 - Analysis of glycan structures on envelope proteins of different species of tick-borne viruses
小林(仁尾)純子, LEE June Chelyn, 比能洋, 平野港, 好井健太朗, 日本ウイルス学会学術集会プログラム・予稿集(Web), 71st, 2024 - 黄体機能マーカーとしてのガレクチンおよびシアル酸転移酵素の発現解析
小林純子, 比能洋, 日本解剖学会総会・全国学術集会抄録集(CD-ROM), 129th, 2024 - Y. pseudotuberculosis O-antigen analysis by MALDI glycotyping
浦上彰吾, 比能洋, 質量分析総合討論会講演要旨集, 72nd, 2024 - O antigen identification by MALDI-MS
浦上彰吾, 比能洋, 日本細菌学雑誌(Web), 78, 1, 2023 - O-glycan analysis of intact glycoprotein by MALDI-ISD MS
浦上彰吾, 比能洋, 質量分析総合討論会講演要旨集, 71st, 2023 - Structural analysis of AFGP by MALDI-TOF MS
福士航生, 深栖啓, 浦上彰吾, 比能洋, 質量分析総合討論会講演要旨集, 71st, 2023 - Development of O Antigen Rapid Typing by MALDI-TOF MS
浦上彰吾, 比能洋, Journal of the Mass Spectrometry Society of Japan, 70, 4, 2022, [Peer-reviewed], [Invited], [Last author]
Report scientific journal, 40247315 - Development of a rapid and simple method for bacterial O antigen identification by MALDI-TOF MS
浦上彰吾, 比能洋, 日本糖質学会年会要旨集, 41st, 2022 - MALDI MSによる簡便・迅速な糖タンパク質糖鎖解析
浦上彰吾, 比能洋, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022 - Development of O antigen rapid typing by MALDI-TOF MS
浦上彰吾, 比能洋, 質量分析総合討論会講演要旨集, 70th, 2022 - シアリル化糖鎖・複合糖質の直接MALDI-TOFMS解析法
比能洋, 日本糖質学会年会要旨集, 38th, 2019 - がん細胞の動的糖鎖修飾の解析によるバイオマーカーの探索
高山理沙, GEBREHIWOT Abrha Gebreselema, 加藤芳規, MARTIN Fayna Maria Garcia, 比能洋, 西村紳一郎, 日本化学会春季年会講演予稿集(CD-ROM), 97th, 2017 - Glycoblotting型糖ペプチドマイクロアレイを用いた構造活性相関研究
比能洋, 比能洋, 日本糖質学会年会要旨集, 36th, 2017 - グライコブロッティング法に基づくリポソーム及びリポたんぱく質中酸化脂質の迅速解析
古川貴之, 比能洋, 武田晴治, 千葉仁志, 西村紳一郎, HUI Shu-Ping, JSBMS Letters, 42, Supplement, 2017 - グライコブロッティング法によるリポタンパク質中の脂質アルデヒド分析
古川貴之, 比能洋, 武田晴治, 千葉仁志, 西村紳一郎, 惠淑萍, 臨床化学, 46, 2017 - Glycoblotting型マイクロアレイによる合成糖ペプチドの相互作用解析
比能洋, 比能洋, 高分子学会予稿集(CD-ROM), 65, 2, 2016 - 脂質アルデヒド類の新規分析法-グライコブロッティング法によるアプローチ:合成標品からリポソームまで
古川貴之, 惠淑萍, 比能洋, 西村紳一郎, 千葉仁志, 臨床化学, 45, 2016 - O-mannosylated glycan induced conformational alteration of alpha-dystroglycan fragment
Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015
English, Summary international conference - Structural insight into glycosylated human Notch1 EGF12 analogs
Shun Hayakawa, Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015
English, Summary international conference - Intracellular traffic of cell surface mimetic quantum dots-anchored glycopeptides
Shin-Ichiro Nishimura, Roger Tan, Kentaro Naruchi, Maho Amano, Hiroshi Hinou, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015
English, Summary international conference - 糖尿病モデルマウスの比較糖鎖解析
坂西裕介, 比能洋, 天野麻穂, 幸田敏明, 上村佳子, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 49th, 2015 - アルコールを用いたペプチド環化反応のpKa依存性について
酒井隆史, 比能洋, 西村紳一郎, 高分子学会予稿集(CD-ROM), 64, 1, 2015 - ブロッティング技術に基づく脂質アルデヒド類の網羅的定量解析
古川貴之, 惠淑萍, 比能洋, 西村紳一郎, 千葉仁志, 日本未病システム学会学術総会抄録集, 22nd, 2015 - Glycoblotting法を用いた牛乳中遊離オリゴ糖の網羅的定量解析
山本祐輔, 古川貴之, 惠淑萍, 比能洋, 西村紳一郎, 千葉仁志, 日本未病システム学会学術総会抄録集, 22nd, 2015 - グライコブロッティング法を用いた脂質アルデヒドの定量測定法の開発
古川貴之, 惠淑萍, 比能洋, 西村紳一郎, 千葉仁志, 臨床化学, 44, 2015 - タウタンパクフラグメントの合成および立体構造解析
横井康広, 早川瞬, 比能洋, 西村紳一郎, 高分子学会予稿集(CD-ROM), 64, 1, 2015 - CMPアジド化シアル酸の合成と糖タンパク質末端の改変
飯田優香, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 49th, 2015 - MRM法によるヒトIgG糖ペプチドフラグメントのキャラクタリゼーション
半村和基, 三好利尚, 成地健太郎, 比能洋, 西村紳一郎, 高分子学会予稿集(CD-ROM), 64, 1, 2015 - DETECTION OF HEMAGGLUTININ DISSOCIATED FROM INFLUENZA VIRUS USING GLYCAN-IMMOBILIZED FIELD EFFECT TRANSISTOR
南部谷俊介, 前川達洋, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 中西卓也, 逢坂哲彌, 逢坂哲彌, Chemical Sensors, 82nd, Supplement A, 2015 - Glycan-diversity focused glycopeptide library
HINOU Hiroshi, PEPTIDE NEWSLETTER JAPAN, 98, 2, 5, 2015, [Invited], [Lead author, Last author, Corresponding author]
Japanese, Introduction other - 量子ドットに提示したCMPシアル酸のライブセルイメージング
平根望巳, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 48th, 2014 - O-Mannose型糖鎖を有する糖ペプチドライブラリの構築及び機能解析に関する研究
菊地星矢, 松下隆彦, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 48th, 2014 - ヒトIgG1抗体に存在するBisect型N-Glycanの効率合成と糖ペプチド合成への応用
石川明香里, RAVI KUMAR H.V., 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 48th, 2014 - Highly efficient synthesis of hyperbranched N-glycans from locust bean gum
H. V. Ravi Kuma, Kentaro Naruchi, Risho Miyoshi, Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 246, Sep. 2013
English, Summary international conference - High-Throughput Antigen Profiling for MUC1 Glycoprotein Antibody
F. Garcia-Martin, T. Matsushita, H. Hinou, S-I Nishimura, BIOPOLYMERS, 100, 3, 269, 269, May 2013
English, Summary international conference - ケイ素保護基を用いた糖ペプチドの迅速固相合成法
藤井宏圭, 松下隆彦, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013 - 糖残基を有するヒトNotch-1EGF-12ドメインの合成と配座解析
脇本行彦, 松下隆彦, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013 - O-Mannose型糖鎖を有するαジストログリカン糖ペプチドライブラリの合成と相互作用解析
菊地星矢, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 93rd, 3, 2013 - O-マンノース含有ジストログリカン関連糖ペプチドの合成と相互作用解析
比能洋, 比能洋, 菊地星矢, 松下隆彦, 西村紳一郎, 西村紳一郎, 高分子学会予稿集(CD-ROM), 62, 1, 2013 - ムチン型糖タンパク質におけるTTXモチーフのNMR構造解析
早川瞬, 松下隆彦, GARCIA-MARTIN Fayna, 成地健太郎, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013 - O-Mannose型糖鎖を有するαジストログリカン糖ペプチドライブラリの合成と相互作用解析
菊地星矢, 比能洋, 比能洋, 松下隆彦, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013 - Autoantibodies to cancer-associated MUC1 fragments in healthy human sera discovered by high performance glycopeptide microarray platform
Hiroshi Hinou, Abstracts of Papers of the American Chemical Society, 246, 2013, [Peer-reviewed] - Rapid Discovery of MUC1 Epitopes by Glycopeptide Combinatorial Chemistry
GARCIA-MARTIN Fayna, MATSUSHITA Takahiko, HINOU Hiroshi, NISHIMURA Shin-Ichiro, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 67, 68, 01 Mar. 2012
English - 糖鎖捕捉反応を用いた新規糖脂質ライブラリの構築と溶液中における構造解析
石田純也, 比能洋, 西村紳一郎, 日本薬学会年会要旨集, 132nd, 2, 2012 - 1C4配座を有する新奇グルクロン酸糖供与体に関する研究
古川貴之, 比能洋, 西村紳一郎, 日本薬学会年会要旨集, 132nd, 2, 2012 - ムチン型糖ペプチドにおけるTTXモチーフのNMR構造解析
早川瞬, 松下隆彦, 比能洋, 西村紳一郎, 日本薬学会年会要旨集, 132nd, 2, 2012 - グライコブロッティング法を用いた異なる種類のヒアルロン酸分解酵素生成ヒアルロン酸断片の同定
荒井みさき, 古川貴之, ガルシア ファイナ, 比能洋, 天野麻穂, 西村紳一郎, 日本薬学会年会要旨集, 132nd, 3, 2012 - An efficient protocol for the solid-phase synthesis of glycopeptides under microwave irradiation
Fayna Garcia-Martin, Hiroshi Hinou, Takahiko Matsushita, Shun Hayakawa, Shin-Ichiro Nishimura, ORGANIC & BIOMOLECULAR CHEMISTRY, 10, 8, 1612, 1617, 2012
English - Exploration of Disease-Specific Epitopes by using MUC1 Glycopeptide Microarray
Wataru Takada, Takahiko Matsushita, Hiroshi Hinou, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 21, 11, 1509, 1510, Nov. 2011
English, Summary international conference - Importance of Sialic Acid Residues Illuminated by Live Animal Imaging Using Phosphorylcholine Self-Assembled Monolayer-Coated Quantum Dots
Tatsuya Ohyanagi, Noriko Nagahori, Ken Shimawaki, Hiroshi Hinou, Tadashi Yamashita, Akira Sasaki, Takashi Jin, Toshihiko Iwanaga, Masataka Kinjo, Shin-Ichiro Nishimura, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133, 32, 12507, 12517, Aug. 2011
English - Novel Coupling Method for the Incorporation of 'Complex' Amino acid
GARCIA-MARTIN Fayna, HINOU Hiroshi, MATSUSHITA Takahiko, NISHIMURA Shin-Ichiro, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2010, 204, 204, 01 Mar. 2011
English - 新規糖脂質ライブラリの構築と構造解析
石田純也, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 45th, 2011 - シアリダーゼ阻害剤の探索技術に関する研究
三好利尚, 甲斐宏一, 高須康明, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 45th, 2011 - 新規糖脂質類縁体ライブラリの構築と構造解析
石田純也, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 91st, 3, 2011 - 環状構造を有する新奇シアリダーゼ自殺基質型阻害剤の開発
甲斐宏一, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 91st, 3, 2011 - 配座変換を鍵戦略とした新規グルクロン酸及びイズロン酸糖供与体の開発
古川貴之, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 91st, 4, 2011 - 疾患特異的エピトープ探索を指向したMUC1糖ペプチドマイクロアレイの開発
松下隆彦, 高田渉, 比能洋, 西村紳一郎, 日本糖質学会年会要旨集, 30th, 2011 - Chemo-enzymatic synthesis of mucin-type glycopeptides using sugar-protective groups and ppGalNAcT-2
Yayoi Yoshimura, Takahiko Matsushita, Naoki Fujitani, Fujihira Haruhiko, Yasuhiro Takegawa, Naomi Manri, Akihito Kaneko, Takeshi Sakamoto, Xiao-Dong Gao, Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 239, Mar. 2010
English, Summary international conference - 新奇シアリダーゼ自殺基質型阻害剤の開発
甲斐宏一, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 90th, 3, 2010 - 高反応性かつ高立体選択性を有する新規グルクロン酸供与体の開発
古川貴之, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 90th, 4, 2010 - ppGalNAcT阻害剤に関する研究
阿部皓基, 比能洋, 比能洋, 古川潤一, 前田哲宏, 細口健作, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009 - シアリダーゼ阻害剤の新規骨格の探索
佐藤公法, 比能洋, 比能洋, 高須康明, 黒河内政樹, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009 - ヒトシアリダーゼの発現と機能解析
奥山力, 高暁冬, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009 - Development of environment-conscious process for glycosylation-Improvement of solid super acid promoter, ZrO2/SO42--
齋藤直裕, 前田哲宏, 松田匡雄, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 1, 2009 - Research on the development of novel structures-based Influenza virus sialidase inhibitors
佐藤公法, 比能洋, 高須康明, 黒河内政樹, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009 - Study of an Inhibitor of ppGalNAcTs
阿部皓基, 比能洋, 古川潤一, 前田哲宏, 細口健作, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009 - Regulation of Glycan Biosynthetic Mechanism by Glycomimics
日向寺慧, 羽藤愛美, 天野麻穂, FENG Fei, 三浦嘉晃, 篠原康郎, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009 - Solid phase glycopeptide synthesis by using Si protecting group
川崎慶, 嶋脇健, 松下隆彦, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009 - 糖ペプチドチップを用いた糖ペプチド-タンパク質間相互作用の解析
松下隆彦, 越智里香, 五十嵐幸太, 比能洋, 西村紳一郎, 繊維学会予稿集, 64, 1, 2009 - mechanism-based labeling of proteins using suicide substrate
古川貴之, 比能洋, FENG Fei, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009 - Structual study of glycosylated EGF12 on mouse Notch-1 receptor
細口健作, 蛭間(清水)和美, 藤谷直樹, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009 - α-ジストログリカン糖ペプチドの効率的合成および機能解析
越智里香, 比能洋, 五十嵐幸太, 松下隆彦, 西村紳一郎, 日本薬学会年会要旨集, 129th, 2, 2009 - ケイ素保護体アミノ酸ビルディングブロックを用いた糖ペプチド固相合成
川崎慶, 嶋脇健, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009 - O-マンノース型糖ペプチドの効率的合成および機能解析
越智里香, 比能洋, 五十嵐幸太, 松下隆彦, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009 - ペプチド型プローブの迅速探索法
古川貴之, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009 - 環状不凍糖タンパク質の合成および不凍活性
八須匡和, 高道学, 比能洋, 清水弘樹, 津田栄, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009 - 癌関連糖ペプチドライブラリーの構築と抗体エピトープマッピングへの応用
松下隆彦, 比能洋, 泉龍孝, 西村紳一郎, 大藪巨樹, 川本敬子, 沼田義人, 十亀弘子, 武本浩, 近藤裕郷, メディシナルケミストリーシンポジウム講演要旨集, 28th, 2009 - NMRによるリニア不凍糖タンパク質(AFGP)のコンフォメーション解析
泉龍孝, 藤谷直樹, 松下隆彦, 比能洋, 高道学, 西宮佳志, 津田栄, 西村紳一郎, 日本糖質学会年会要旨集, 29th, 2009 - 新規不凍糖タンパク質誘導体の合成と性質
八須匡和, 比能洋, 高道学, 三浦信明, 清水弘樹, 津田栄, 西村紳一郎, 西村紳一郎, 日本糖質学会年会要旨集, 29th, 2009 - Mechanism-based probing, characterization, and inhibitor design of glycosidases and glycosyltransferases
Hiroshi Hinou, Shin-Ichiro Nishimura, Current Topics in Medicinal Chemistry, 9, 1, 106, 116, 2009, [Peer-reviewed], [Lead author]
1, English, Introduction scientific journal, 10270150 - シアリダーゼ自殺基質におけるアグリコンフォーカスドライブラリーの作製と評価
甲斐宏一, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 2008 - 多糖類誘導体の整形外科領域での高次利用
比能洋, 西村紳一郎, 岩崎倫政, 三浪明男, 繊維学会予稿集, 63, 1/2, 2008 - 酵素の動的構造に着目したシアリダーゼ阻害剤の開発研究
高須康明, 黒河内政樹, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 2008 - フォーカスドライブラリを用いた特異的シアル酸転移酵素阻害剤の探索
細口健作, 古川潤一, 前田哲宏, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 2008 - 大規模高速糖鎖構造解析法の開発と応用
天野麻穂, 比能洋, 古川潤一, 篠原康郎, 西村紳一郎, 日本化学会講演予稿集, 88th, 2, 2008 - O-マンノース型糖ペプチドの効率的合成および機能解析に関する研究
越智里香, 比能洋, 五十嵐幸太, 西村紳一郎, 日本化学会講演予稿集, 88th, 2, 2008 - 腫瘍マーカーに対する糖鎖修飾が誘導する立体構造変化
藤谷直樹, 黒河内政樹, GAO Xiao-Dong, 松下隆彦, 比能洋, 篠原康郎, 西村紳一郎, Abstracts. Annual Meeting of the NMR Society of Japan, 46th, 2007 - ポリラクトサミン含有複合糖質のライブラリー構築と構造解析
成地健太郎, 浜本智樹, 黒河内政樹, 松下隆彦, 藤谷直樹, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 41st, 2007 - Construction of tumor - Related gkycpeptided library
Shin Ichiro Nishimura, Hiroshi Hinou, Hiroki Shimizu, Takahiko Matsushita, Naoki Oyabu, Masataka Fumoto, Hirosato Kondo, Polymer Preprints, Japan, 55, 5508, 01 Dec. 2006 - Efficient syntheses and characterizations of MUC1 related high-molecular weight glycopeptides
Takahiko Matsushita, Toshiki Ohyabu, Toshiki Ohyabu, Hiroshi Hinou, Hiroki Shimizu, Shin Ichiro Nishimura, Shin Ichiro Nishimura, Polymer Preprints, Japan, 55, 1857, 19 Oct. 2006 - CARB 63-One-pot solid phase glycoblotting and labeling by trans-oximization for high-throughput glycomics and glycoproteomics
Hideyuki Shimaoka, Jun-ichi Furukawa, Yoshiaki Miura, Masaki Kurogochi, Hiroshi Hinou, Yasuro Shinohara, Kisaburo Deguchi, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 232, Sep. 2006
English, Summary international conference - ダイナミックな作用機序に基づく糖転移酵素阻害剤の分子設計法
西村紳一郎, 西村紳一郎, 高谷健二, 前田哲宏, 細口健作, 古川潤一, 比能洋, 日本糖質学会年会要旨集, 26th, 2006 - 癌関連糖ペプチドライブラリの構築
西村紳一郎, 比能洋, 清水弘樹, 松下隆彦, 大藪巨樹, 麓昌高, 近藤裕郷, 高分子学会予稿集(CD-ROM), 55, 2 Disk1, 2006 - MUC1関連高分子糖ペプチドの効率的合成と機能評価
松下隆彦, 大藪巨樹, 大藪巨樹, 比能洋, 清水弘樹, 西村紳一郎, 西村紳一郎, 高分子学会予稿集(CD-ROM), 55, 1 Disk1, 2006 - 酵素連続反応による糖鎖自動合成法 糖鎖自動合成装置Golgiの開発
西村紳一郎, 清水弘樹, 比能洋, 産総研TODAY, 6, 2, 2006 - 自殺基質によるシアリダーゼ解析
比能洋, 黒河内政樹, 秋場聡, 武川泰啓, 西村紳一郎, 日本糖質学会年会要旨集, 26th, 2006 - 糖鎖自動合成装置"Golgi"の開発と糖ペプチド合成システムへの展開 (第23回 バイオテクノロジー シンポジウム 予稿集) -- (講演会の部)
比能 洋, バイオテクノロジ-シンポジウム予稿集, 23, 15, 18, 22 Nov. 2005
バイオテクノロジ-開発技術研究組合, Japanese - 糖ペプチド合成用プライマーの開発と糖ペプチドライブラリーへの応用 (第23回 バイオテクノロジー シンポジウム 予稿集) -- (ポスターセッションの部 糖鎖エンジニアリングプロジェクト/糖鎖構造解析技術開発)
麓 昌高, 比能 洋, 清水 弘樹, バイオテクノロジ-シンポジウム予稿集, 23, 164, 166, 22 Nov. 2005
バイオテクノロジ-開発技術研究組合, Japanese - 糖ペプチド合成用プライマーの開発と糖ペプチドライブラリーへの応用
麓昌高, 麓昌高, 比能洋, 比能洋, 清水弘樹, 太田尚志, 太田尚志, 松下隆彦, 岩田一道, 山田久里子, 山田久里子, 伊藤孝臣, 大藪巨樹, 大藪巨樹, 瀧本明生, 近藤裕郷, 稲津敏行, 中原義昭, 西村紳一郎, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 23rd, 2005 - 糖鎖自動合成装置“Golgi”用糖転移酵素の開発と利用
岩田一道, 太田尚志, 太田尚志, 山田久里子, 山田久里子, 戸田篤志, 戸田篤志, 比能洋, 西村紳一郎, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 23rd, 2005 - 糖転移酵素Och1pの機能解析に関する研究
清水弘樹, 坂本雅博, 坂本雅博, 千葉靖典, 喜多島敏彦, 地神芳文, 比能洋, 西村紳一郎, 西村紳一郎, 日本農芸化学会大会講演要旨集, 2005, 2005 - Development and application of the water-soluble primer for rapid and efficient glycopeptides synthesis
麓昌高, 比能洋, 太田尚志, 伊藤孝臣, 瀧本明生, 近藤裕郷, 稲津敏行, 中原義昭, 西村紳一郎, 日本化学会講演予稿集, 85th, 2, 2005 - Development of a Mechanism-based Inhibitor of Neuraminidases
比能洋, 黒河内政樹, 長堀紀子, 西村紳一郎, 日本化学会講演予稿集, 85th, 2, 2005 - Solid-phase glycopeptide synthesis under Microwave irradiation
松下隆彦, 比能洋, 清水弘樹, 西村紳一郎, 日本化学会講演予稿集, 85th, 2, 2005 - 光学活性スルホキシドを用いる不斉Favorskii転位反応を利用した光学活性化合物の合成
三浦基文, 鳥山正晴, 比能洋, 浅見覚, 鈴木孝, 佐藤毅, 本橋重康, 日本薬学会年会要旨集, 125th, 4, 2005 - 糖鎖自動合成装置“Golgi”の開発
山田久里子, 山田久里子, 太田尚志, 太田尚志, 岩田一道, 麓昌高, 麓昌高, 大藪巨樹, 大藪巨樹, 比能洋, 清水弘樹, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 23rd, 2005 - 糖鎖化学の最先端技術
シーエムシー出版, HH003053, 2005, [Invited]
Japanese - 糖鎖自動合成装置“Golgi”にむけた糖ペプチド合成用プライマーの開発
岩田一道, 麓昌高, 比能洋, 太田尚志, 松下隆彦, 山田久里子, 伊藤孝臣, 瀧本明生, 稲津敏行, バイオテクノロジーシンポジウム予稿集, 22nd, 2004 - 不斉Favorskii転位反応を用いた光学活性アミンの合成
大東彩子, 比能洋, 浅見覚, 鳥山正晴, 鈴木孝, 本橋重康, 日本薬学会年会要旨集, 124th, 2, 2004 - 糖鎖自動合成装置“Golgi”による複合糖質の合成
山田久里子, 戸田篤志, 西口進, 岩田一道, 麓昌高, 比能洋, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 22nd, 2004 - 糖鎖自動合成技術の開発研究および,糖ペプチドライブラリー合成への展開
比能洋, 太田尚志, 山田久里子, 西口進, 戸田篤志, 岩田一道, 地神芳文, 松下隆彦, 新間陽一, 高分子学会予稿集(CD-ROM), 53, 2 Disk1, 2004 - 糖鎖自動合成法に関する基礎研究(X)
西口進, 戸田篤志, 山田久里子, 太田尚志, 比能洋, 新間陽一, 地神芳文, 西村紳一郎, 高分子学会予稿集(CD-ROM), 53, 2 Disk1, 2004 - PTPase阻害薬を用いた小児悪性腫ようへの応用
宮本智子, 浅見覚, 堀川あすか, 大東彩子, 比能洋, 鳥山正晴, 本橋重康, 鈴木孝, 日本薬学会年会要旨集, 124th, 4, 2004 - 糖鎖自動合成装置“Golgi”用固定化糖転移酵素の開発
戸田篤志, 山田久里子, 比能洋, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 22nd, 2004 - 二基質型シアル酸転移酵素阻害剤の系統的合成
比能洋, 本橋重康, 伊藤幸成, 日本薬学会年会要旨集, 123rd, 2, 2003 - 二基質型シアル酸転移酵素阻害剤の合成研究
比能洋, 孫学龍, 本橋重康, 伊藤幸成, 次世代を担う有機化学シンポジウム講演要旨集, 1st, 2003 - 不斉Favorskii転位反応を用いた光学活性カルボン酸の合成
吉原久美子, 比能洋, 鳥山正晴, 本橋重康, 日本薬学会年会要旨集, 123rd, 2, 2003 - Synthetic Study of Bisubstrate-Type Sialyltransferase Inhibitors. (4).
比能洋, 伊藤幸成, 日本化学会講演予稿集, 81st, 2, 2002 - 二基質結合型シアル酸転移酵素阻害剤の合成研究
比能洋, SUN X-L, 伊藤幸成, 日本糖質学会年会要旨集, 23rd, 2002 - Synthetic Studies on Bisubstrate Type Slalyltransferase Inhibitors.
比能洋, SUN X-L, 伊藤幸成, 日本化学会講演予稿集, 79th, 2, 2001 - 二基質結合型シアル酸転移酵素阻害剤の合成研究 (2)
比能洋, SUN X L, 伊藤幸成, 日本糖質学会年会要旨集, 22nd, 2001 - Development of a convergent method for the construction of polycyclic ethers.
松尾剛, 上田剛, 比能洋, 越野広雪, 中田忠, 日本化学会講演予稿集, 79th, 2, 2001 - 2基質結合型シアル酸転移酵素阻害剤の合成
比能洋, 生体分子の化学講演要旨集, 5th, 2001 - Efficient convergent synthesis of a trans-fused tetracyclic ether ring system.
松尾剛, 比能洋, 越野広雪, 末永俊朗, 中田忠, 日本化学会講演予稿集, 78th, 2, 2000 - 多環状エーテルの高効率的合成法の開発
中田忠, 松尾剛, 堀伸行, 松倉弘子, 比能洋, 反応と合成の進歩シンポジウム講演要旨集, 26th, 2000 - Novel Synthesis of L-Iduronic Acid Using Trehalose as Starting Material.
比能洋, 黒沢英弘, 松岡浩司, 照沼大陽, 葛原弘美, 日本化学会講演予稿集, 76th, 2, 1999 - Chemical Conversion of Trehalose into L-idose-containing Disaccharides.
比能洋, 黒沢英弘, 松岡浩司, 照沼大陽, 葛原弘美, 日本化学会講演予稿集, 74th, 2, 1998 - Synthesis of amphiphatic chitoheptasaccharide derivative.
比能洋, 海野篤, 松岡浩司, 照沼大陽, 葛原弘美, 糖質シンポジウム講演要旨集, 19th, 1997 - Synthesis of amphipathic chitooligosaccharide derivatives. I. Glycosidation reactions utilyzing a long chain acylamino group.
海野篤, 比能洋, 松岡浩司, 照沼大陽, 葛原弘美, 日本化学会講演予稿集, 72nd, 2, 1997 - Synthesis of amphipathic chitooligosaccharide derivatives. II. Synthesis of tri-, penta-, and hepta-saccharide derivatives having a pair of long hydrophobic chains.
比能洋, 海野篤, 松岡浩司, 照沼大陽, 葛原弘美, 日本化学会講演予稿集, 72nd, 2, 1997 - Synthesis of Chitobiose Derivatives with Micell Formation Potency.
比能洋, 海野篤, 松岡浩司, 照沼大陽, 葛原弘美, キチン・キトサン研究, 3, 2, 1997 - Studies on the Synthesis of Chitooligosaccharide Derivatives as Potential Bioactive Micelle.
比能洋, 海野篤, 松岡浩司, 照沼大陽, 葛原弘美, 日本化学会講演予稿集, 70th, 2, 1996
Books and other publications
Lectures, oral presentations, etc.
- Glycomics Focused Glycomic Biomarker Discovery and Identification (Glycotyping)
Hiroshi Hinou
ION Conference, Addis Ababa, Ethiopia, 01 Sep. 2025, English, Invited oral presentation
01 Sep. 2025 - 02 Sep. 2025, 48686282, [Invited] - Glycotyping: A highway of chemical glycobiology
Hiroshi Hinou
Special Lecture of Carbohydrate Chemistry Dpt. Chem. IIT Bombay, 31 May 2025, English, Public discourse
48686282, [Invited] - MALDI Glycotyping: New strategy for diagnosis and biotyping
Hiroshi Hinou
One-Day International Conference on "Science and Technology for a Sustainable Tomorrow: Innovation and Pioneering Breakthroughs, 30 May 2025, English, Keynote oral presentation
48686282, [Invited] - Functional and Structural Glycotyping starting from Automated glycosynthesizer and nalizer
Hiroshi Hinou
NATIONAL LEVEL SCI-TECH FEST-5.0, 29 May 2025, English, Invited oral presentation
48686282, [Invited] - Structural and functional glycotyping: the cutting edge of molecular medicine
Hiroshi Hinou
International Conference on New Challenges and Innovations in Molecular Medicine, 28 May 2025, English, Invited oral presentation
48686282, [Invited] - Glycotyping: Frontier Glycoscience Starting from Automated Glycosynthesis and Glycosequencing Study
Hifroshi Hinou
Double Conference Session of “Chemical Biology” & “Peptides4Life”, 11 Feb. 2025, English, Nominated symposium
[Invited] - Frontier Molecular Life Science Network Driven by Glycotyping
Hiroshi Hinou
JSPS Core-to-Core Program Ethiopia–India–Japan "Frontier Molecular Life Science Network Driven by Glycotyping", 06 Sep. 2024, English, Nominated symposium - Structural and functional glycotyping using MALDI-MS and microarray
Hiroshi Hinou
I GlycoForum: La Rioja-Euskadi-Hokkaido,, 07 Sep. 2023, English, Nominated symposium
40247315, [Invited] - Glycotyping: Old and New Concept of Glycoscience
Hiroshi Hinou
108th Indian Science Congress, 06 Jan. 2023, English, Keynote oral presentation
04 Jan. 2023 - 07 Jan. 2023, 40247315, [Invited] - MALDI Glycotyping of Glycoproteins, Biofluids, and Bacterial O-antigens
Hiroshi Hinou
International Congress on Pure and Applied Chemistry (ICPAC) Kota Kinabalu 2022, 25 Nov. 2022, English, Invited oral presentation
40247315, [Invited] - 糖鎖選択的イオン化技術（質量分析）、糖鎖機能探索技術（マイクロアレイ）
比能洋
イノベーション・ジャパン2021, 23 Aug. 2021, Japanese, Public symposium
23 Aug. 2021 - 19 Sep. 2021, 29295082 - MALDI-TOFMSによる糖質解析 ～前処理工程をどれだけ簡素化できるか～
比能 洋
質量分析学会第29回北海道談話会, 05 Feb. 2021, Invited oral presentation
[Invited] - 化学者から見た糖鎖研究
比能 洋
異分野meetup week2020☆研究者コミュニティサロン☆“糖鎖研究について語ろう！, 02 Dec. 2020, Nominated symposium
[Invited] - マイクロアレイおよび質量分析による糖鎖・複合糖質の機能及び構造解析
比能 洋
畜産とキチンキトサン勉強会, 16 Nov. 2020, Invited oral presentation
[Invited] - Chemical Glycobiology (Starting from Glycoconjugate Syntheses)
Hiroshi Hinou
107th Indian Science Congress, 06 Jan. 2020, English, Invited oral presentation
29295082, [Invited] - 糖ペプチドの迅速合成・品質管理・利用に関する基盤技術の構築
比能 洋
糖化学フォーラム, 26 Oct. 2019, Invited oral presentation
[Invited] - 糖を介して見る生命の世界
比能 洋
北大道新アカデミー, 10 Oct. 2019, Public discourse - Novel matrix system for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides
Hiroshi Hinou
Japanese-American Symposium on Applied & Translational Glycosciences, ACS Fall 2019 National Meeting, 27 Aug. 2019, English, Invited oral presentation
29295082, [Invited] - Microarray and NMR Study of Core M1 Glycan Modified α-Dystroglycan Fragments
Hiroshi Hinou
International Congress on Pure and Applied Chemistry 2018, 08 Mar. 2018, English, Invited oral presentation
29295082, [Invited] - グライコブロッティング法に基づくリポソーム及びリポたんぱく質中酸化脂質の迅速解析
古川貴之, 比能洋, 武田晴治, 千葉仁志, 西村紳一郎, HUI Shu‐Ping
JSBMS Letters, 25 Aug. 2017, Japanese - がん細胞の動的糖鎖修飾の解析によるバイオマーカーの探索
高山理沙, GEBREHIWOT Abrha Gebreselema, 加藤芳規, MARTIN Fayna, Maria Garcia, 比能洋, 西村紳一郎
日本化学会春季年会講演予稿集(CD-ROM), 03 Mar. 2017, Japanese - Glycoblotting型マイクロアレイによる合成糖ペプチドの相互作用解析
比能洋, 比能洋
高分子学会予稿集(CD-ROM), 24 Aug. 2016, Japanese, Invited oral presentation
[Invited], [Domestic Conference] - Glycoblotting法を用いた牛乳中遊離オリゴ糖の網羅的定量解析
山本祐輔, 古川貴之, 惠淑萍, 比能洋, 西村紳一郎, 千葉仁志
日本未病システム学会学術総会抄録集, 10 Sep. 2015, Japanese - MRM法によるヒトIgG糖ペプチドフラグメントのキャラクタリゼーション
半村和基, 三好利尚, 成地健太郎, 比能洋, 西村紳一郎
高分子学会予稿集(CD-ROM), 12 May 2015, Japanese - DETECTION OF HEMAGGLUTININ DISSOCIATED FROM INFLUENZA VIRUS USING GLYCAN-IMMOBILIZED FIELD EFFECT TRANSISTOR
南部谷俊介, 前川達洋, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 中西卓也, 逢坂哲彌
Chem Sens, 15 Mar. 2015, Japanese - 糖鎖固定化電界効果トランジスタによるインフルエンザウイルスから分離したヘマグルチニンの検出
南部谷俊介, 前川達洋, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 中西卓也, 逢坂哲彌
電気化学会大会講演要旨集(CD-ROM), 09 Mar. 2015, Japanese - Synthesis and Application of Bicyclic Carbohydrates
Hiroshi Hinou
International Symposium on Chemical Biology-Drug Discovery, 10 Jan. 2014, English, Invited oral presentation
10270150, [Invited] - ヒトIgG1抗体に存在するBisect型N‐Glycanの効率合成と糖ペプチド合成への応用
ISHIKAWA AKARI, RAVI KUMAR H.V, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
高分子学会北海道支部研究発表会講演要旨集, 2014, Japanese - O‐Mannose型糖鎖を有する糖ペプチドライブラリの構築及び機能解析に関する研究
KIKUCHI SEIYA, MATSUSHITA TAKAHIKO, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
高分子学会北海道支部研究発表会講演要旨集, 2014, Japanese - 量子ドットに提示したCMPシアル酸のライブセルイメージング
HIRANE NOZOMI, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
高分子学会北海道支部研究発表会講演要旨集, 2014, Japanese - New Strategy for Peptide Cyclization H-bonding effect of fluorinated alcohol
Hiroshi Hinou
Special Seminar;at Chemistry Department of Wane;State University, 06 Sep. 2013, English, Public discourse
10270149, [Invited] - O‐マンノース含有ジストログリカン関連糖ペプチドの合成と相互作用解析
HINO HIROSHI, KIKUCHI SEIYA, MATSUSHITA TAKAHIKO, NISHIMURA SHIN'ICHIRO
高分子学会予稿集(CD-ROM), 14 May 2013, Japanese, Invited oral presentation
[Invited], [Domestic Conference] - O‐Mannose型糖鎖を有するαジストログリカン糖ペプチドライブラリの合成と相互作用解析
KIKUCHI SEIYA, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, Mar. 2013, Japanese, Oral presentation - 糖残基を有するヒトNotch‐1EGF‐12ドメインの合成と配座解析
WAKIMOTO YUKIHIKO, MATSUSHITA TAKAHIKO, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
高分子学会北海道支部研究発表会講演要旨集, 2013, Japanese - O‐Mannose型糖鎖を有するαジストログリカン糖ペプチドライブラリの合成と相互作用解析
KIKUCHI SEIYA, HINO HIROSHI, MATSUSHITA TAKAHIKO, NISHIMURA SHIN'ICHIRO
高分子学会北海道支部研究発表会講演要旨集, 2013, Japanese, Oral presentation - ムチン型糖タンパク質におけるTTXモチーフのNMR構造解析
HAYAKAWA SHUN, MATSUSHITA TAKAHIKO, GARCIA-MARTIN FAYNA, NARUCHI KENTARO, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
高分子学会北海道支部研究発表会講演要旨集, 2013, Japanese, Oral presentation - ケイ素保護基を用いた糖ペプチドの迅速固相合成法
FUJII HIROYOSHI, MATSUSHITA TAKAHIKO, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
高分子学会北海道支部研究発表会講演要旨集, 2013, Japanese - リバースグライコブロッティング法を利用した腎癌糖タンパク質マーカーの探索(Exploration of novel diagnosis markers of renal cancer utilized by reverse glycoblotting method)
天野 麻穂, 畠山 真吾, 三好 利尚, 比能 洋, 大山 力, 西村 紳一郎
日本癌学会総会記事, Aug. 2012, English - グライコブロッティング法を用いた異なる種類のヒアルロン酸分解酵素生成ヒアルロン酸断片の同定
荒井みさき, 古川貴之, ガルシア ファイナ, 比能洋, 天野麻穂, 西村紳一郎
日本薬学会年会要旨集, 05 Mar. 2012, Japanese - Rapid Discovery of MUC1 Epitopes by Glycopeptide Combinatorial Chemistry
GARCIA-MARTIN Fayna, MATSUSHITA Takahiko, HINOU Hiroshi, NISHIMURA Shin-Ichiro
Peptide science : proceedings of the ... Japanese Peptide Symposium, 01 Mar. 2012, English - Novel Coupling Method for the Incorporation of 'Complex' Amino acid
GARCIA-MARTIN Fayna, HINOU Hiroshi, MATSUSHITA Takahiko, NISHIMURA Shin-Ichiro
Peptide science : proceedings of the ... Japanese Peptide Symposium, 01 Mar. 2011, English - Peptide Cyclization in Fluorinated Alcohol: Toward Cyclic AFGP
Hiroshi Hinou
Seminario Excepcional de Grupo-Chemistry and Molecular Pharmacology Programme, 17 Sep. 2010, English, Public discourse
10270149, [Invited] - Novel Strategies for Neuraminidase Inhibitors using Suicide Substrates
Hiroshi Hinou
Barcelona Biomed Seminer-Chemistry and Molecular Pharmacology Programme, 02 Sep. 2010, English, Public discourse
10270150, [Invited] - 癌関連糖ペプチドライブラリーの構築と抗体エピトープマッピングへの応用
松下隆彦, 比能洋, 泉龍孝, 西村紳一郎, 大藪巨樹, 川本敬子, 沼田義人, 十亀弘子, 武本浩, 近藤裕郷
メディシナルケミストリーシンポジウム講演要旨集, 10 Nov. 2009, Japanese - NMRによるリニア不凍糖タンパク質(AFGP)のコンフォメーション解析
泉龍孝, 藤谷直樹, 松下隆彦, 比能洋, 高道学, 西宮佳志, 津田栄, 西村紳一郎
日本糖質学会年会要旨集, 10 Aug. 2009, Japanese - Regulation of Glycan Biosynthetic Mechanism by Glycomimics
HYUGAJI SATOSHI, HATO MEGUMI, AMANO MAHO, FENG FEI, MIURA YOSHIAKI, SHINOHARA YASURO, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 13 Mar. 2009, Japanese - Research on the development of novel structures-based Influenza virus sialidase inhibitors
SATO KIMINORI, HINO HIROSHI, TAKASU YASUAKI, KUROGOUCHI MASAKI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 13 Mar. 2009, Japanese - Structual study of glycosylated EGF12 on mouse Notch-1 receptor
HOSOGUCHI KENSAKU, HIRUMA(SHIMIZU, KAZUMI, FUJITANI NAOKI, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 13 Mar. 2009, Japanese - mechanism-based labeling of proteins using suicide substrate
FURUKAWA TAKAYUKI, HINO HIROSHI, FENG FEI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 13 Mar. 2009, Japanese - Development of environment-conscious process for glycosylation-Improvement of solid super acid promoter, ZrO2/SO42--
SAITO NAOHIRO, MAEDA TETSUHIRO, MATSUDA MASAO, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 13 Mar. 2009, Japanese - Solid phase glycopeptide synthesis by using Si protecting group
KAWASAKI KEI, SHIMAWAKI KEN, MATSUSHITA TAKAHIKO, HINO HIROSHI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 13 Mar. 2009, Japanese - Study of an Inhibitor of ppGalNAcTs
ABE HIROKI, HINO HIROSHI, FURUKAWA JUN'ICHI, MAEDA TAKAHIRO, HOSOGUCHI KENSAKU, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 13 Mar. 2009, Japanese - 環状不凍糖タンパク質の合成および不凍活性
八須匡和, 高道学, 比能洋, 清水弘樹, 津田栄, 西村紳一郎, 西村紳一郎
高分子学会北海道支部研究発表会講演要旨集, 03 Feb. 2009, Japanese - シアリダーゼ阻害剤の新規骨格の探索
佐藤公法, 比能洋, 比能洋, 高須康明, 黒河内政樹, 西村紳一郎, 西村紳一郎
高分子学会北海道支部研究発表会講演要旨集, 03 Feb. 2009, Japanese - ppGalNAcT阻害剤に関する研究
阿部皓基, 比能洋, 比能洋, 古川潤一, 前田哲宏, 細口健作, 西村紳一郎
高分子学会北海道支部研究発表会講演要旨集, 03 Feb. 2009, Japanese - フォーカスドライブラリを用いた特異的シアル酸転移酵素阻害剤の探索
細口健作, 古川潤一, 前田哲宏, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎
高分子学会北海道支部研究発表会講演要旨集, 29 Jan. 2008, Japanese - 酵素の動的構造に着目したシアリダーゼ阻害剤の開発研究
高須康明, 黒河内政樹, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎
高分子学会北海道支部研究発表会講演要旨集, 29 Jan. 2008, Japanese - シアリダーゼ自殺基質におけるアグリコンフォーカスドライブラリーの作製と評価
甲斐宏一, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎
高分子学会北海道支部研究発表会講演要旨集, 29 Jan. 2008, Japanese - 腫瘍マーカーに対する糖鎖修飾が誘導する立体構造変化
藤谷直樹, 黒河内政樹, GAO Xiao‐Dong, 松下隆彦, 比能洋, 篠原康郎, 西村紳一郎
Abstr Annu Meet NMR Soc Jpn, 11 Sep. 2007, Japanese - ポリラクトサミン含有複合糖質のライブラリー構築と構造解析
成地健太郎, 浜本智樹, 黒河内政樹, 松下隆彦, 藤谷直樹, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎
高分子学会北海道支部研究発表会講演要旨集, 06 Feb. 2007, Japanese - Construction of tumor - Related gkycpeptided library
Shin Ichiro Nishimura, Hiroshi Hinou, Hiroki Shimizu, Takahiko Matsushita, Naoki Oyabu, Masataka Fumoto, Hirosato Kondo
Polymer Preprints, Japan, 01 Dec. 2006
Combined use of a solid-phase chemical synthesis of peptide and enzymatic synthesis of carbohydrate on water soluble polymer allowed for the construction of complicated glycopeptide library which contributes to functional analysis of the glycopeptides. This study was supported by NEDO. - Efficient syntheses and characterizations of MUC1 related high-molecular weight glycopeptides
Takahiko Matsushita, Toshiki Ohyabu, Toshiki Ohyabu, Hiroshi Hinou, Hiroki Shimizu, Shin Ichiro Nishimura, Shin Ichiro Nishimura
Polymer Preprints, Japan, 19 Oct. 2006
The mucin-type glycoprotein MUC1 is known to be aberrant glycosylated in various cancer cells. We report the efficient method for the syntheses of MUC1-related glycopeptides having different sugar chains based on core2-type trisaccharide structure by combination of microwave-assisted solid-phase syntheses and enzymatic sugar elongation. Characterizations by MUC1 antibody assay will also be discussed. - ダイナミックな作用機序に基づく糖転移酵素阻害剤の分子設計法
西村紳一郎, 西村紳一郎, 高谷健二, 前田哲宏, 細口健作, 古川潤一, 比能洋
日本糖質学会年会要旨集, 28 Jul. 2006, Japanese - MUC1関連高分子糖ペプチドの効率的合成と機能評価
松下隆彦, 大藪巨樹, 大藪巨樹, 比能洋, 清水弘樹, 西村紳一郎, 西村紳一郎
高分子学会予稿集(CD-ROM), 10 May 2006, Japanese - 糖鎖自動合成装置“Golgi”用糖転移酵素の開発と利用
岩田一道, 太田尚志, 太田尚志, 山田久里子, 山田久里子, 戸田篤志, 戸田篤志, 比能洋, 西村紳一郎, 西村紳一郎
バイオテクノロジーシンポジウム予稿集, 22 Nov. 2005, Japanese - 糖ペプチド合成用プライマーの開発と糖ペプチドライブラリーへの応用
麓昌高, 麓昌高, 比能洋, 比能洋, 清水弘樹, 太田尚志, 太田尚志, 松下隆彦, 岩田一道, 山田久里子, 山田久里子, 伊藤孝臣, 大藪巨樹, 大藪巨樹, 瀧本明生, 近藤裕郷, 稲津敏行, 中原義昭, 西村紳一郎, 西村紳一郎
バイオテクノロジーシンポジウム予稿集, 22 Nov. 2005, Japanese - 糖鎖自動合成装置“Golgi”の開発
山田久里子, 山田久里子, 太田尚志, 太田尚志, 岩田一道, 麓昌高, 麓昌高, 大藪巨樹, 大藪巨樹, 比能洋, 清水弘樹, 西村紳一郎
バイオテクノロジーシンポジウム予稿集, 22 Nov. 2005, Japanese - 糖ペプチド合成用プライマーの開発と糖ペプチドライブラリーへの応用 (第23回 バイオテクノロジー シンポジウム 予稿集) -- (ポスターセッションの部 糖鎖エンジニアリングプロジェクト/糖鎖構造解析技術開発)
麓 昌高, 比能 洋, 清水 弘樹
バイオテクノロジ-シンポジウム予稿集, 22 Nov. 2005, Japanese - 糖鎖自動合成装置"Golgi"の開発と糖ペプチド合成システムへの展開 (第23回 バイオテクノロジー シンポジウム 予稿集) -- (講演会の部)
比能 洋
バイオテクノロジ-シンポジウム予稿集, Nov. 2005, Japanese
[Invited] - Solid-phase glycopeptide synthesis under Microwave irradiation
MATSUSHITA TAKAHIKO, HINO HIROSHI, SHIMIZU HIROKI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 11 Mar. 2005, Japanese - Development of a Mechanism-based Inhibitor of Neuraminidases
HINO HIROSHI, KUROGOUCHI MASAKI, NAGAHORI NORIKO, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 11 Mar. 2005, Japanese - Development and application of the water-soluble primer for rapid and efficient glycopeptides synthesis
FUMOTO MASATAKA, HINO HIROSHI, OTA TAKASHI, ITO TAKAOMI, TAKIMOTO AKIO, KONDO HIROSATO, INAZU TOSHIYUKI, NAKAHARA YOSHIAKI, NISHIMURA SHIN'ICHIRO
日本化学会講演予稿集, 11 Mar. 2005, Japanese - 光学活性スルホキシドを用いる不斉Favorskii転位反応を利用した光学活性化合物の合成
三浦基文, 鳥山正晴, 比能洋, 浅見覚, 鈴木孝, 佐藤毅, 本橋重康
日本薬学会年会要旨集, 05 Mar. 2005, Japanese - 糖転移酵素Och1pの機能解析に関する研究
清水弘樹, 坂本雅博, 坂本雅博, 千葉靖典, 喜多島敏彦, 地神芳文, 比能洋, 西村紳一郎, 西村紳一郎
日本農芸化学会大会講演要旨集, 05 Mar. 2005, Japanese - 糖鎖自動合成装置“Golgi”による複合糖質の合成
山田久里子, 戸田篤志, 西口進, 岩田一道, 麓昌高, 比能洋, 西村紳一郎
バイオテクノロジーシンポジウム予稿集, 04 Nov. 2004, Japanese - 糖鎖自動合成装置“Golgi”にむけた糖ペプチド合成用プライマーの開発
岩田一道, 麓昌高, 比能洋, 太田尚志, 松下隆彦, 山田久里子, 伊藤孝臣, 瀧本明生, 稲津敏行
バイオテクノロジーシンポジウム予稿集, 04 Nov. 2004, Japanese - 糖鎖自動合成装置"Golgi"にむけた糖ペプチド合成用プライマーの開発 (第22回バイオテクノロジーシンポジウム予稿集) -- (ポスターセッションの部 糖鎖エンジニアリングプロジェクト/糖鎖構造解析技術開発)
岩田 一道, 麓 昌高, 比能 洋
バイオテクノロジ-シンポジウム予稿集, 04 Nov. 2004, Japanese - 糖鎖自動合成法に関する基礎研究(X)
西口進, 戸田篤志, 山田久里子, 太田尚志, 比能洋, 新間陽一, 地神芳文, 西村紳一郎
高分子学会予稿集(CD-ROM), 01 Sep. 2004, Japanese - 糖鎖自動合成技術の開発研究および,糖ペプチドライブラリー合成への展開
比能洋, 太田尚志, 山田久里子, 西口進, 戸田篤志, 岩田一道, 地神芳文, 松下隆彦, 新間陽一
高分子学会予稿集(CD-ROM), Sep. 2004, Japanese, Invited oral presentation
[Invited], [Domestic Conference] - PTPase阻害薬を用いた小児悪性腫ようへの応用
宮本智子, 浅見覚, 堀川あすか, 大東彩子, 比能洋, 鳥山正晴, 本橋重康, 鈴木孝
日本薬学会年会要旨集, 05 Mar. 2004, Japanese - PTPase阻害薬を用いた小児悪性腫瘍への応用
宮本 智子, 浅見 覚, 堀川 あすか, 大東 彩子, 比能 洋, 鳥山 正晴, 本橋 重康, 鈴木 孝
日本薬学会年会要旨集, Mar. 2004, Japanese - PTPase阻害剤による小児悪性腫瘍への応用
浅見 覚, 宮本 智子, 伊藤 リエ, 堀川 あすか, 鶴見 洋子, 三宅 宗晴, 大東 彩子, 比能 洋, 鳥山 正晴, 木村 由美子, 秋久 俊博, 佐藤 毅, 本橋 重康, 陳 基明, 麦島 秀雄, 鈴木 孝
小児がん, Nov. 2003, Japanese - 糖鎖エンジニアリングプロジェクト/糖鎖構造解析技術開発 糖鎖自動合成装置のさらなる改良に向けて
西村紳一郎, 比能洋
バイオテクノロジーシンポジウム予稿集, Nov. 2003, Japanese, Invited oral presentation
[Invited], [Domestic Conference] - 二基質型シアル酸転移酵素阻害剤の合成研究
比能洋, 孫学龍, 本橋重康, 伊藤幸成
次世代を担う有機化学シンポジウム講演要旨集, May 2003, Japanese, Invited oral presentation
[Invited], [Domestic Conference] - Synthetic Study of Bisubstrate-Type Sialyltransferase Inhibitors. (4).
HINO HIROSHI, ITO YUKISHIGE
日本化学会講演予稿集, 11 Mar. 2002, Japanese - 2基質結合型シアル酸転移酵素阻害剤の合成
比能洋
生体分子の化学講演要旨集, Nov. 2001, Japanese, Invited oral presentation
[Invited], [Domestic Conference] - Synthetic Studies on Bisubstrate Type Slalyltransferase Inhibitors.
HINO HIROSHI, SUN X-L, ITO YUKISHIGE
日本化学会講演予稿集, 15 Mar. 2001, Japanese - Development of a convergent method for the construction of polycyclic ethers.
MATSUO GO, UEDA TSUYOSHI, HINO HIROSHI, KOSHINO HIROYUKI, NAKATA TADASHI
日本化学会講演予稿集, 15 Mar. 2001, Japanese - Efficient convergent synthesis of a trans-fused tetracyclic ether ring system.
MATSUO GO, HINO HIROSHI, KOSHINO HIROYUKI, SUENAGA TOSHIRO, NAKATA TADASHI
日本化学会講演予稿集, 15 Mar. 2000, Japanese - Novel Synthesis of L-Iduronic Acid Using Trehalose as Starting Material.
HINO HIROSHI, KUROSAWA HIDEHIRO, MATSUOKA KOJI, TERUNUMA DAIYO, KUZUHARA HIROYOSHI
日本化学会講演予稿集, 15 Mar. 1999, Japanese - Chemical Conversion of Trehalose into L-idose-containing Disaccharides.
HINO HIROSHI, KUROSAWA HIDEHIRO, MATSUOKA KOJI, TERUNUMA DAIYO, KUZUHARA HIROYOSHI
日本化学会講演予稿集, Mar. 1998, Japanese - Synthesis of amphiphatic chitoheptasaccharide derivative.
HINO HIROSHI, UMINO ATSUSHI, MATSUOKA KOJI, TERUNUMA DAIYO, KUZUHARA HIROYOSHI
糖質シンポジウム講演要旨集, Jul. 1997, Japanese - Synthesis of Chitobiose Derivatives with Micell Formation Potency.
HINO HIROSHI, UMINO ATSUSHI, MATSUOKA KOJI, TERUNUMA DAIYO, KUZUHARA HIROMI
キチン・キトサン研究, 15 May 1997, Japanese - Synthesis of amphipathic chitooligosaccharide derivatives. II. Synthesis of tri-, penta-, and hepta-saccharide derivatives having a pair of long hydrophobic chains.
HINO HIROSHI, UMINO ATSUSHI, MATSUOKA KOJI, TERUNUMA DAIYO, KUZUHARA HIROYOSHI
日本化学会講演予稿集, Mar. 1997, Japanese - Synthesis of amphipathic chitooligosaccharide derivatives. I. Glycosidation reactions utilyzing a long chain acylamino group.
UMINO ATSUSHI, HINO HIROSHI, MATSUOKA KOJI, TERUNUMA DAIYO, KUZUHARA HIROYOSHI
日本化学会講演予稿集, Mar. 1997, Japanese - Studies on the Synthesis of Chitooligosaccharide Derivatives as Potential Bioactive Micelle.
HINO HIROSHI, UMINO ATSUSHI, MATSUOKA KOJI, TERUNUMA DAIYO, KUZUHARA HIROYOSHI
日本化学会講演予稿集, Mar. 1996, Japanese
Courses
- Bio-Organic Chemistry
Hokkaido University
Oct. 2024 - Present - Transdisciplinary Life Science/Soft Matter Science
Hokkaido University
Apr. 2023 - Present - The World of Science and Technology Frontier in Life Science
Hokkaido University
Oct. 2020 - Present - 科学・技術の世界（はじめての生命科学II-研究の最前線-）
北海道大学
Feb. 2020 - 実験生物科学
北海道大学 - 有機化学実験
東京理科大学 - 化学実験
日本大学 - 糖鎖生物学
北海道大学 - 生体高分子学基礎実験
北海道大学 - 生体高分子学実験
北海道大学 - 有機化学演習
北海道大学 - 応用生物有機化学
北海道大学 - 生物系のための有機化学
北海道大学 - 基礎有機化学
北海道大学 - 環境と人間(生命科学の最前線)
北海道大学 - 科学・技術の世界（はじめての生命科学から「医」への展開）
北海道大学
Affiliated academic society
Research Themes
- Frontier Molecular Life Science Network Driven by Glycotyping
JSPS Core-to-Core Program Asia-Africa Type
Apr. 2024 - Mar. 2027
JSPS, Principal investigator, JPJSCCB20240004 - Development of MALDI glycotyping technology
Grants-in-Aid for Scientific Research
01 Apr. 2022 - 31 Mar. 2026
比能 洋, 小林 純子
本年度は糖鎖選択的イオン化技術の基盤強化を図るとともに、測定対象分子の拡張のための基盤構築と実証研究を実施した。また、昨年度までに構築した微生物O抗原の迅速分析技術の検体数拡大を図ると共に、糖鎖選択敵的イオン化技術を活用したMALDIイメージングのための基盤構築を行った。まず、糖鎖選択的イオン化技術の理論実証のため、1991年に世界初の糖鎖MALDI-MS解析用マトリックスとして報告された3-Amino-4-hydroxybenzoic Acid (AHBA)に着目した直接糖鎖解析技術を開発した。代表者は2,5-dihydroxybenzoic acid (DHB)とアニリン誘導体混合物を基盤とした糖鎖選択的イオン化技術を開発したが、AHBAはDHBとアニリンの構造を併せ持つ分子である。しかし、AHBAはDHBと比較すると糖鎖のイオン化効率が低いためMALDIマトリックスとしての利用されなくたった。我々はAHBAが代表者の研究コンセプト基盤となったペプチド等より糖鎖選択性の高いイオン化能を有していることを見出し、アルカリ金属添加により、その特性を先鋭化した。さらに、レーザー照射に伴うイオン生成量が低いことを活用し、強レーザー照射によるインソース分解を組み合わせた糖タンパク質糖鎖直接解析に最適であることを見出し、無処理生物ムチンからのO-結合型（ムチン型）糖鎖構造パターンの直接解析に世界で初めて成功した。また、MALDIグリコタイピング対象拡大のため、従来の糖鎖捕捉技術glycoblottingと弱イオン交換技術を高度に融合した硫酸化およびリン酸化糖鎖の迅速識別分析技術の構築に成功した。さらに、水鳥卵白糖鎖のリン酸化と硫酸化に着目した大規模グライコミクス研究を行い、糖鎖の硫酸化とリン酸化が水鳥を自然宿主とするインフルエンザウイルスの水鳥種間罹患率と相関することを見出した。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23K23458 - Development of MALDI glycotyping technology targeting a wide range of biological samples.
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2022 - 31 Mar. 2026
比能 洋, 小林 純子
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 22H02191 - 黄体の機能制御に重要な糖鎖の探索～alpha2,6シアル酸修飾に注目して
科学研究費助成事業 基盤研究(C)
Apr. 2021 - Mar. 2024
小林 純子, 工藤 正尊, 比能 洋
黄体は妊娠の成立と維持に必須なプロゲステロンを産生する内分泌組織で、排卵後の卵胞壁細胞より形成される。妊娠が成立しない場合、ヒトでは、黄体は1週間ほど機能したのち、自発的に退行する。一方、妊娠が成立すると、胎盤より産生されるヒト絨毛性ゴナドトロピン（hCG）の作用により、黄体は退行を免れ妊娠黄体となって妊娠初期の数か月間機能を維持する。マウスやウシなどの動物では、子宮由来の因子が黄体の退行を誘導するが、ヒトでは子宮由来の因子は卵巣周期に影響を与えない。ヒトでは、黄体内で産生される因子が自発的に黄体の退行を制御すると考えられるがそのメカニズムは不明な点が多く残されている。
我々は、ヒト黄体では、beta-galactosideを認識するガレクチンのうち、galectin-1が機能黄体に、galectin-3が退行黄体に発現しており、ガレクチンと糖鎖との相互作用が機能制御に重要な役割を果たすことを報告してきた。ガレクチンと糖鎖との結合を阻害するalpha2,6シアル酸修飾は退行黄体で増加することから、ヒト黄体の機能制御に重要な役割を果たすと考えられるが、alpha2,6シアル酸修飾された糖鎖をもつタンパク質やその機能の詳細は明らかでない。
本研究では、ヒト黄体細胞において、退行時にalpha2,6シアル酸修飾をうける糖タンパク質を同定し、その機能を明らかにするとともに、質量分析イメージング装置を用いた切片上で糖鎖を検出できる技術の確立を目指し、黄体の機能制御に重要な糖鎖を探索することを目的とする。
日本学術振興会, 基盤研究(C), 北海道大学, 21K06727 - Antibodies targeting dynamic epitopes generated by posttranslational protein glycosylation
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
01 Apr. 2019 - 31 Mar. 2023
西村 紳一郎, 田中 良和, 比能 洋, 尾瀬 農之
申請者らの独創的なアイデアおよび開発戦略によって作製されたED抗体（epitope-defined antibody）を用いて、がん領域におけるアンメットメディカルニーズに応え得る新たながん治療薬の開発を目的として本研究課題を設定した。2019年度（初年度）は多くのがん細胞表面に高発現してがんの増殖や転移を促進することが広く知られるMUC1の細胞外ドメインに生成する多様な動的エピトープを標的とするED抗体である抗MUC1モノクローナル抗体（SN-101, SN-121, およびSN-131）に関して以下の各項目に進展があった。（１）SN-101の作製、特性、構造および機能に関する基礎的研究の成果について論文を発表した（H. Wakui, et al., A straightforward approach to antibodies recognizing cancer specific glycopeptidic neoepitopes, Chemical Science, 2020, in press）。本論文は水溶液中でユニークなコンフォメーションを示す糖ペプチドエピトープの糖鎖とペプチド領域を同時に認識して結合する抗MUC1抗体として構造が示された世界初の事例であり、ED-抗体の概念と基本的な製法を記載した最初の論文としての意義が大きい。また、（２）SN-121およびSN-131についても同様の構造機能解析が進んでおり、SN-131の結晶構造解析を完了した。これらの抗体の高付加価値化を目指してエピトープ認識領域での変異導入による機能改変の試みが開始されている。さらに、（３）SN-131に新たな機能（がん細胞障害活性）を付与するため遺伝子工学的手法により新たな二重特異性抗体の作製を行い、MUC1発現がん細胞を標的として特異的な細胞障害活性を示す新規SN-131キメラ抗体の開発に成功した。以上のように本年度はED-抗体開発戦略の有効性とdynamic epitope理論に基づく新しい抗体デザインに関する基本原理である「抗原構造形成における新たな分子機構」の普遍性を証明する様々な研究が極めて順調に進行している。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 19H00918 - Analysis of immunological properties of mannose-based molecules and its action mechanisms.
Grants-in-Aid for Scientific Research
01 Apr. 2019 - 31 Mar. 2022
Toda Masako
The aim of this study was to identify immunological function of mannose-based molecules and its action mechanisms. Among α-mannooligosaccharides, long chain molecules such as α-Man-(1→6)-Man4 inhibited lipopolysaccharide (LPS)-stimulated activation of bone marrow derived murine dendritic cells (BMDC). In contrast, among β-mannooligosaccharides, β-Man-(1→4)-Man activated BMDC via engagement of toll-like receptor 4. We also found that α-mannan from yeast promotes anti-inflammatory responses (e.g., IL-10 production) in BMDC when it was stimulated with LPS. Metabolome analysis showed that α-mannan altered levels of many metabolites in BMDC, e.g., an increased level of lactic acid, the final product of glycolysis, and a reduced level of succinic acid, an inflammatory metabolite of TCA cycle. The results suggest that α-mannan induces reprogram of cellular metabolism and thereby promotes anti-inflammatory response in BMDC.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Tohoku University, 19H02902 - Correlation analysis of antigen glycosylation with immune response to infectious diseases
A-STEP
Apr. 2021 - Mar. 2022
Hiroshi Hinou
JST, Hokkaido Univ. - Innovation of manufacturing and processing technology of chitosan oligosaccharides by mass control
A-STEP
Nov. 2020 - Mar. 2022
Hiroshi Hinou
JST, Principal investigator - Multidimensional investigation of Glycopeptide-lectin interaction
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2017 - 31 Mar. 2020
Hinou Hiroshi
Regarding the interaction between glycopeptides and lectins, mucin-type glycopeptides and O-mannose-type glycopeptides were used as models for interaction analysis by microarray and conformational analysis by NMR. In mucin-type glycopeptides, hydrogen bonds and hydrophobic interactions between glycans and peptides are strongly involved in conformations of both sugar chains and peptides. Both of the glycan and peptide moiety is markedly restricted near the binding sites of the glycan on the peptides. It was found that the binding property of lectin changes with the conformational change. On the other hand, it was confirmed that the O-mannose type glycopeptide does not cause conformational restriction of glycan, but that the conformational restriction of peptides occurs with the addition of a specific sugar residue. Corresponding to this peptide conformational change, the NMR signal and lectin binding pattern also altered.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 17K05920 - Exploration of Novel Markers on Cancer and Development of a Technology for the Diagnosis Utilized by Comprehensive Glycomics
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S)
31 May 2013 - 31 Mar. 2018
Nishimura Shin-Ichiro
To establish the first disease-related glycome database, we have challenged the large-scale glycomics using more than 3500 patient’s sera provided from clinical teams. An innovative “automated glycan analytical system” facilitated rapid and large-scale serum glycomics and quantitative glycan profiling toward new biomarker discovery. For example, we succeeded in the discovery of novel N-glycan biomarkers as a significant recurrence factor based on whole serum glycan profiling of 369 patients of hepatocellular carcinoma(HCC)(Hepatology 2013 57, 2314-2325; Hepatology 2014 59, 355-356), N-glycans as promising prognostic biomarkers in renal cell carcinoma(The Prostate 2014 74, 1521-1529), and N-glycans associated significantly with metastatic castration-resistant status in prostate cancer(J. Urology 2014 191, 805-813). These results allowed the construction of the first database of the disease-related glycome feasible for the development of new diagnostic and therapeutic technologies.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (S), Hokkaido University, 25220206 - ２糖資源を活用した２糖アミノ酸調製法
A-STEP機能検証フェーズ（旧・地域産学バリュープログラム）(A-STEP機能検証フェーズ)
Jun. 2015 - Mar. 2016
比能洋
科学技術振興機構(JST), 北海道大学, Principal investigator - Control of membrane fouling based on structures of polysaccharides revealed by application of glyco-blotting
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2012 - 31 Mar. 2015
KIMURA Katsuki, HINOU Hiroshi, AIZAWA Tomoyasu
Enrichment of polysaccharides by glyco-blotting, in which polysaccharides are specifically purified via interactions between the aldehydes in the polysaccharides and aminooxy groups on glycoblotting beads, enabled MALDI-TOF/MS analysis at a high resolution. Structures of polysaccharides extracted from fouled membranes used in a pilot-scale MBR and those in the supernatant of the mixed liquor suspension in the MBR were investigated. It was demonstrated that the overlap between polysaccharides found in the supernatants and those extracted from the fouled membrane was limited, implying that polysaccharides that dominate in supernatants may not be important in membrane fouling in MBRs. Analysis using a bacterial carbohydrate database suggested that capsular polysaccharides (CPS) and/or lipo-polysaccharides (LPS) play an important role in the evolution of membrane fouling in MBRs.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 24360212 - Study for rapid synthesis and mechanism elucidation of cyclic antifreeze glycopeptide
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2011 - 31 Mar. 2014
HINOU HIROSHI
Cyclic anti-freeze glydopeptide (AFGP) found by our group has different conformation from linear AFGP found from polar fish. To elucidate the structural feature of the cyclic AFGP, we developed novel cyclization method for peptide and glycopeptide by use of hydrogen-bonding property of fluorinated alcohols. Pure cyclic AFGP was obtained by the new method in high yield from solution of corresponding corresponding linear precursor. Interestingly, common cyclization method using DPPA induce C-terminal epimerization. NMR and X-ray crystallographic experiments for cyclic AFGP and corresponding cyclic peptide skeleton revealed anti-parallel beta-sheet structure of the both compound, and glycan modification induce a replacement of amino acid position in the structure.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 23350074 - Development of novel compound of function selective labeling reagent for proteins and its systematic use
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)
2007 - 2009
HINOU Hiroshi
I have tried to develop a novel method of inhibitor design of such enzymes exemplified to a neuraminidase Vibrio cholerae neuraminidase (VCNA) of which crystal structure was reported in 1994 and most potent inhibitor was reported in 1971. As a result, a potent inhibitor for Vibrio cholerae neuramnidase was developed using mechanism-based labeling information of key amino acid residues, design of a skeleton of focused library based on labeling information, screening of the focused library, and elimination of promiscuous inhibitor by detergent-based assay to afford potent (Ki=73nM) and selective inhibitor among viral, bacterial, and mammal neuraminidases.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (A), Hokkaido University, Principal investigator, Competitive research funding, 19685015 - Function search of glycoconjugates by selective laser desorption ionization mass spectronmeter using metal nanoparticle
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
2005 - 2007
NISIMURA Shin-ichitro, NAGAHORI Noriko, HINOU Hiroshi
this research was performed to understand the functions of glycoconjugates at the molecular level, that is to say, to develop a new methodology for hunting and identifying the partner molecules with target carbohydrates by using the gold colloidal nanoparticle and the mass spectrometer.
in this study, the modification of nanoparticles was performed using specific affinity with a metallic surface and thiol group. Strong interaction of carbohydrate-displaying nanoparticle with a partner molecule is expected, because several hundred of carbohydrate molecules are displayed onto a surface of nanoparticle (carbohydrate cluster effect). In addition, the system that could display any carbohydrates was built by using chemoselective reaction with aminooxy1 group and a reducing end of carbohydrate. A library of carbohydrate presentation nano particle was made using this system. In addition, the surfaces of the nanoparticles were modified with suicide substrate type carbohydrate derivatives that could anchor the target proteins with covalent bonds. The presentation of carbohydrates on the nanoparticles was evaluated by MALDI-TOF MS using that the laser cleaved metal-thiol bond. In order to reduce the non-specific adhesion of proteins, a thiol compound with zwitterionic head group was designed and synthesized. All carbohydrate-displaying metal nanoparticles prepared in this study showed high water solubility and stability.
The highly water-soluble nanoparticles could be directly subjected to a various biochemical assays and MALDI-TOF MS measurement after the capture of partner molecules. Incubate nanoparticle and sample solution containing partner molecules, and wash the particle with buffer, then the particle was just applied to SDS-PAGE. The protein identification was possible according to the ordinary method of proteomics. In this way, a high sensitivity by reduced sample loss and a promotion of efficiency by simplification of activity process were achieved, compared with traditional approaches such as the affinity chromatography which needed elution by a competing carbohydrate.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 17205015 - 糖鎖・糖ペプチド自動合成システムを活用した創薬シーズの探索研究
2006
Competitive research funding - 酵素阻害剤の開発
2000
Competitive research funding - 天然有機資源の有効活用
1994
Competitive research funding
Industrial Property Rights
- MＡＬＤＩ－ＴＯＦＭＳによる複合糖質高分子糖鎖の直接解析方法およびそれに用いるための固体マトリックス組成物
Patent right, 比能 洋, 北海道大学
特願2021-000822, 2021
特許7594307, 26 Nov. 2024
36102632 - 未修飾シアリル化複合糖質および糖ペプチドのリフレクトロンモードＭＡＬＤＩ－ＴＯＦおよびＴＯＦ／ＴＯＦ質量分析のためのアニリン誘導体またはアミノオキシ基含有芳香族誘導体／ＤＨＢ／アルカリ金属マトリックス組成物
Patent right, 比能 洋
特願PCT/JP2020/002624, Jan. 2020 - Novel matrix for MALDI-TOFMS of unmodified sialylated compounds
Patent right, Hiroshi Hinou, Hokkaido University
特願2019-011457, 25 Jan. 2019 - 糖化合物固定化半導体センシングデバイス及び生物学的物質の検出方法
Patent right, 逢坂 哲彌, 秀島 翔, 黒岩 繁樹, 西村 紳一郎, 比能 洋, 逢坂 哲彌
特願2012-191701, 31 Aug. 2012
特開2013-152211, 08 Aug. 2013
特許第6019944号
14 Oct. 2016
201603006914839016 - 抗MUC1抗体
Patent right, 西村 紳一郎, 比能 洋, 沼田 義人, 小野田 順二, 内藤 正一, 大藪 巨樹, 塩野義製薬株式会社, 国立大学法人北海道大学
特願2010-535828, 28 Oct. 2009
特許第5773352号
10 Jul. 2015
201503005608873550 - 糖化合物固定化半導体センシングデバイス及び生物学的物質の検出方法
Patent right, 逢坂 哲彌, 秀島 翔, 黒岩 繁樹, 西村 紳一郎, 比能 洋, 学校法人早稲田大学
特願2012-191701, 31 Aug. 2012
特開2013-152211, 08 Aug. 2013
201303031273044877 - O−結合型糖アミノ酸
Patent right, 西村 紳一郎, 比能 洋, 西口 進, 浜本 智樹, 独立行政法人産業技術総合研究所, 国立大学法人北海道大学
特願2005-264181, 12 Sep. 2005
特開2006-111618, 27 Apr. 2006
特許第4942014号
09 Mar. 2012
201303039837534498 - 抗MUC1抗体
Patent right, 西村 紳一郎, 比能 洋, 沼田 義人, 小野田 順二, 内藤 正一, 大藪 巨樹, 塩野義製薬株式会社, 国立大学法人北海道大学
JP2009068531, 28 Oct. 2009
WO2010-050528, 06 May 2010
201203074594144478 - O−マンノース型糖鎖結合アミノ酸及びそれを用いた糖ペプチドの製造方法
Patent right, 西村 紳一郎, 比能 洋, 国立大学法人 北海道大学
特願2008-059657, 10 Mar. 2008
特開2009-215207, 24 Sep. 2009
200903095867572860 - シアリダーゼ阻害剤
Patent right, 西村 紳一郎, 比能 洋, 国立大学法人北海道大学, 塩野義製薬株式会社
JP2008060639, 11 Jun. 2008
WO2009-004899, 08 Jan. 2009
201003067533838050 - シアリダーゼ阻害剤
Patent right, 西村 紳一郎, 比能 洋, 国立大学法人 北海道大学
特願2007-133705, 21 May 2007
特開2008-285448, 27 Nov. 2008
200903075477968851 - 銀被覆粒子およびその用途
Patent right, 岩田 一道, 比能 洋, 西村 紳一郎, 独立行政法人産業技術総合研究所
JP2006310186, 23 May 2006
WO2006-126504, 30 Nov. 2006
200903067315142698 - ノイラミニダーゼ阻害剤
Patent right, 西村 紳一郎, 比能 洋, 近藤 裕郷, 藤原 一彦, 独立行政法人産業技術総合研究所, 国立大学法人 北海道大学, 塩野義製薬株式会社, 住友ベークライト株式会社
JP2005023162, 16 Dec. 2005
WO2006-064914, 22 Jun. 2006
200903022388768940 - O−結合型糖アミノ酸
Patent right, 西村 紳一郎, 比能 洋, 西口 進, 浜本 智樹, 独立行政法人産業技術総合研究所, 国立大学法人 北海道大学, 東洋紡績株式会社, ヤマサ醤油株式会社
特願2005-264181, 12 Sep. 2005
特開2006-111618, 27 Apr. 2006
200903084311417074 - ムチン型ペプチドの合成法とMUC1関連糖ペプチド
Patent right, 西村 紳一郎, 比能 洋, 麓 昌高, 独立行政法人産業技術総合研究所, 塩野義製薬株式会社
JP2005016975, 14 Sep. 2005
WO2006-030840, 23 Mar. 2006
200903006544391902 - O−アセチル化糖アミノ酸
Patent right, 西村 紳一郎, 比能 洋, 独立行政法人産業技術総合研究所
特願2004-250954, 30 Aug. 2004
特開2006-063055, 09 Mar. 2006
200903025121684320 - 糖ペプチドの製造方法
Patent right, 西村 紳一郎, 比能 洋, 松下 隆彦, 独立行政法人産業技術総合研究所
特願2004-213351, 21 Jul. 2004
特開2006-028141, 02 Feb. 2006
200903000287255557
