Sep. 2020, Institute for the Advancement of Higher Education, Excellent teacher　　　　　　　　　　　　　　　
Biology II
Shigeyuki Koshikawa

Nov. 2016, The Zoological Society of Japan, Narishige Zoological Science Award　　　　　　　　　　　　　　　
Mechanism and evolution of color pattern formation in a polka-dotted fruit fly, Drosophila guttifera
Shigeyuki Koshikawa

Aug. 2016, Society of Evolutionary Studies, Japan, Young Scientist Initiative Award　　　　　　　　　　　　　　　
昆虫の新奇形質に関する発現機構の解明
Shigeyuki Koshikawa

Apr. 2014, Kyoto University, Hakubi Researcher (5th batch)　　　　　　　　　　　　　　　
Shigeyuki Koshikawa

Thermal plasticity of wing size and wing spot size in Drosophila guttifera
Yuichi Fukutomi, Aya Takahashi, Shigeyuki Koshikawa
Development Genes and Evolution, 233, 77, 89, Dec. 2023, [Peer-reviewed], [Last author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal

Cis-regulatory evolution underlying the changes in wingless expression pattern associated with wing pigmentation of Drosophila
Takumi Karasawa, Namoho Saito, Shigeyuki Koshikawa
FEBS Letters, 597, 14, 1837, 1847, Jul. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal

Photoreceptor genes in a trechine beetle, Trechiama kuznetsovi, living in the upper hypogean zone
Takuma Niida, Yuto Terashima, Hitoshi Aonuma, Shigeyuki Koshikawa
Zoological Letters, 9, 9, 12 May 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

ショウジョウバエの翅の模様形成メカニズムとその進化　　　　　　　　　　　　　　　
柄澤匠, 越川滋行
科学, 92, 11, 992, 996, 01 Nov. 2022, [Invited], [Last author, Corresponding author]
Japanese, Scientific journal

昆虫の翅模様の進化発生生物学　　　　　　　　　　　　　　　
古関将斗, 越川滋行
生体の科学, 73, 4, 364, 367, 公益財団法人金原一郎記念医学医療振興財団／医学書院, 15 Aug. 2022, [Invited], [Last author, Corresponding author], [Domestic magazines]
Japanese, Scientific journal

Construction of a massive genetic resource by transcriptome sequencing and genetic characterization of Megasyllis nipponica (Annelida: Syllidae)
Yoshinobu Hayashi, Kohei Oguchi, Mayuko Nakamura, Shigeyuki Koshikawa, Toru Miura
Genes & Genetic Systems, 01 Jun. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal

Diversity of melanin synthesis genes in insects
Ryo Futahashi, Shigeyuki Koshikawa, Genta Okude, Mizuko Osanai-Futahashi
Advances in Insect Physiology, 62, 339, 376, Elsevier, 18 Apr. 2022, [Peer-reviewed], [Invited], [International Magazine]
English, In book

Hormone-related genes heterochronically and modularly regulate neotenic differentiation in termites
Kohei Oguchi, Shigeyuki Koshikawa, Toru Miura
Developmental Biology, 485, 70, 79, 04 Mar. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal

シロアリの幼形生殖虫分化における内分泌関連遺伝子の 異時的・部位特異的な制御
小口 晃平, 越川 滋行, 三浦 徹
比較内分泌学, 44, 176, e0044, 2022, [Peer-reviewed], [Invited]
Japanese, Scientific journal

DrosoPhyla: resources for Drosophilid Phylogeny and Systematics
Cédric Finet, Victoria A Kassner, Antonio B Carvalho, Henry Chung, Jonathan P Day, Stephanie Day, Emily K Delaney, Francine C De Ré, Héloïse D Dufour, Eduardo Dupim, Hiroyuki F Izumitani, Thaísa B Gautério, Jessa Justen, Toru Katoh, Artyom Kopp, Shigeyuki Koshikawa, Ben Longdon, Elgion L Loreto, Maria D S Nunes, Komal K B Raja, Mark Rebeiz, Michael G Ritchie, Gayane Saakyan, Tanya Sneddon, Machiko Teramoto, Venera Tyukmaeva, Thyago Vanderlinde, Emily E Wey, Thomas Werner, Thomas M Williams, Lizandra J Robe, Masanori J Toda, Ferdinand Marlétaz
Genome Biology and Evolution, evab179, Oxford University Press (OUP), 03 Aug. 2021, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal,
The vinegar fly Drosophila melanogaster is a pivotal model for invertebrate development, genetics, physiology, neuroscience, and disease. The whole family Drosophilidae, which contains over 4,400 species, offers a plethora of cases for comparative and evolutionary studies. Despite a long history of phylogenetic inference, many relationships remain unresolved among the genera, subgenera and species groups in the Drosophilidae. To clarify these relationships, we first developed a set of new genomic markers and assembled a multilocus dataset of 17 genes from 704 species of Drosophilidae. We then inferred a species tree with highly supported groups for this family. Additionally, we were able to determine the phylogenetic position of some previously unplaced species. These results establish a new framework for investigating the evolution of traits in fruit flies, as well as valuable resources for systematics.

Mechanism of Color Pattern Formation in Insects
Yuichi Fukutomi, Shigeyuki Koshikawa
Pigments, Pigment Cells and Pigment Patterns, 367, 384, 06 Jul. 2021, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

Transcriptome analysis reveals wingless regulates neural development and signaling genes in the region of wing pigmentation of a polka-dotted fruit fly.
Yuichi Fukutomi, Shu Kondo, Atsushi Toyoda, Shuji Shigenobu, Shigeyuki Koshikawa
The FEBS journal, 288, 1, 115, 126, 04 Jan. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, How evolutionary novelties have arisen is one of the central questions in evolutionary biology. Pre-existing gene regulatory networks or signaling pathways have been shown to be co-opted for building novel traits in several organisms. However, the structure of entire gene regulatory networks and evolutionary events of gene co-option for emergence of a novel trait are poorly understood. In this study, to explore the genetic and molecular bases of the novel wing pigmentation pattern of a polka-dotted fruit fly (Drosophila guttifera), we performed de novo genome sequencing and transcriptome analyses. As a result, we comprehensively identified the genes associated with the pigmentation pattern. Furthermore, we revealed that 151 of these associated genes were positively or negatively regulated by wingless, a master regulator of wing pigmentation. Genes for neural development, Wnt signaling, Dpp signaling, and effectors (such as enzymes) for melanin pigmentation were included among these 151 genes. None of the known regulatory genes that regulate pigmentation pattern formation in other fruit fly species were included. Our results suggest that the novel pigmentation pattern of the polka-dotted fruit fly might have emerged through multi-step co-options of multiple gene regulatory networks, signaling pathways, and effector genes, rather than recruitment of one large gene circuit.

No evidence for contribution of sexually monomorphic wing pigmentation pattern to mate choice in Drosophila guttifera
Takuma Niida, Shigeyuki Koshikawa
Ethology, 127, 7, 527, 536, 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

The color pattern inducing gene wingless is expressed in specific cell types of campaniform sensilla of a polka-dotted fruit fly, Drosophila guttifera
Masato Koseki, Nobuaki K. Tanaka, Shigeyuki Koshikawa
Development Genes and Evolution, 231, 85, 93, 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

Enhancer functions underlying morphological diversity
Takayuki Suzuki, Shigeyuki Koshikawa
Development, Growth & Differentiation, 62, 5, 263, 264, Jun. 2020, [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

Temporal flexibility of gene regulatory network underlies a novel wing pattern in flies
Héloïse D. Dufour, Shigeyuki Koshikawa, Cédric Finet
Proceedings of the National Academy of Sciences of the United States of America, 117, 21, 11589, 11596, 11 May 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, 共同責任著者

The making of transgenic Drosophila guttifera
Mujeeb Shittu, Tessa Steenwinkel, Shigeyuki Koshikawa, Thomas Werner
Methods and Protocols, 3, 31, 27 Apr. 2020, [Peer-reviewed], [Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal

Evolution of wing pigmentation in Drosophila: Diversity, physiological regulation, and cis-regulatory evolution
Shigeyuki Koshikawa
Development, Growth & Differentiation, 62, 5, 269, 278, 14 Mar. 2020, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author], [International Magazine]
English, Scientific journal

Life cycle of the Japanese green syllid, Megasyllis nipponica (Annelida: Syllidae): Field collection and establishment of rearing system
Miura T, Oguchi K, Nakamura M, Jimi N, Miura S, Hayashi Y, Koshikawa S, Aguado MT
Zoological Science, 36, 5, 372, 379, Oct. 2019, [Peer-reviewed], ［Internationally co-authored］, [Domestic magazines]
English, Scientific journal

Thoughts on function of fly wing color pattern (In Japanese)
Takuma Niida, Shigeyuki Koshikawa
The Nature and Insects, 54, 7, 36, 39, Jul. 2019, [Invited], [Last author, Corresponding author], [Domestic magazines]
Japanese, Scientific journal, In Japanese

Reduction of a nymphal instar in a dampwood termite: heterochronic shift in the caste differentiation pathways
Nii R, Oguchi K, Shinji J, Koshikawa S, Miura T
EvoDevo, 10, 10, May 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Termite soldier mandibles are elongated by dachshund under hormonal and Hox gene controls
Sugime Y, Oguchi K, Gotoh H, Hayashi Y, Matsunami M, Shigenobu S, Koshikawa S, Miura T
Development, 146, 5, dev171942, Mar. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Modular cis-regulatory logic of yellow gene expression in silkmoth larvae
Suzuki TK, Koshikawa S, Kobayashi I, Uchino K, Sezutsu H
Insect Molecular Biology, 28, 4, 568, 577, Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Present and future of research on pattern formation of insects
Yuichi Fukutomi, Shigeyuki Koshikawa
SANSHI-KONCHU BIOTEC, 87, 2, 95, 102, Aug. 2018, [Invited], [Last author, Corresponding author], [Domestic magazines]
Japanese, Scientific journal, In Japanese

Methods for staging pupal periods and measurement of wing pigmentation of Drosophila guttifera
Fukutomi Y, Matsumoto K, Funayama N, Koshikawa S
Journal of Visualized Experiments, 131, e56935, Jan. 2018, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

Drosophila guttifera as a model system for unraveling color pattern formation
Koshikawa S, Fukutomi Y, Matsumoto K
Diversity and Evolution of Butterfly Wing Patterns: An Integrative Approach, 287, 301, Sep. 2017, [Peer-reviewed], [Invited], [Lead author, Corresponding author], [International Magazine]
English, In book

Pupal development and pigmentation process of a polka-dotted fruit fly, Drosophila guttifera (Insecta, Diptera)
Yuichi Fukutomi, Keiji Matsumoto, Kiyokazu Agata, Noriko Funayama, Shigeyuki Koshikawa
DEVELOPMENT GENES AND EVOLUTION, 227, 3, 171, 180, SPRINGER, Jun. 2017, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Various organisms have color patterns on their body surfaces, and these color patterns are thought to contribute to physiological regulation, communication with conspecifics, and signaling with the environment. An adult fly of Drosophila guttifera (Insecta: Diptera: Drosophilidae) has melanin pigmentation patterns on its body and wings. Though D. guttifera has been used for research into color pattern formation, how its pupal development proceeds and when the pigmentation starts have not been well studied. In this study, we defined the pupal stages of D. guttifera and measured the pigment content of wing spots from the pupal period to the period after eclosion. Using a transgenic line which carries eGFP connected with an enhancer of yellow, a gene necessary for melanin synthesis, we analyzed the timing at which the yellow enhancer starts to drive eGFP. We also analyzed the distribution of Yellow-producing cells, as indicated by the expression of eGFP during pupal and young adult periods. The results suggested that Yellow-producing cells were removed from wings within 3 h after eclosion, and wing pigmentation continued without epithelial cells. Furthermore, the results of vein cutting experiments showed that the transport of melanin precursors through veins was necessary for wing pigmentation. These results showed the importance of melanin precursors transported through veins and of extracellular factors which were secreted from epithelial cells and left in the cuticle.

Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
Takuma Hashimoto, Daiki D. Horikawa, Yuki Saito, Hirokazu Kuwahara, Hiroko Kozuka-Hata, Tadasu Shin-, Yohei Minakuchi, Kazuko Ohishi, Ayuko Motoyama, Tomoyuki Aizu, Atsushi Enomoto, Koyuki Kondo, Sae Tanaka, Yuichiro Hara, Shigeyuki Koshikawa, Hiroshi Sagara, Toru Miura, Shin-ichi Yokobori, Kiyoshi Miyagawa, Yutaka Suzuki, Takeo Kubo, Masaaki Oyama, Yuji Kohara, Asao Fujiyama, Kazuharu Arakawa, Toshiaki Katayama, Atsushi Toyoda, Takekazu Kunieda
NATURE COMMUNICATIONS, 7, 12808, NATURE PUBLISHING GROUP, Sep. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by similar to 40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms.

Phylogeography of the Subgenus Drosophila (Diptera: Drosophilidae): Evolutionary History of Faunal Divergence between the Old and the New Worlds
Hiroyuki F. Izumitani, Yohei Kusaka, Shigeyuki Koshikawa, Masanori J. Toda, Toru Katoh
PLOS ONE, 11, 7, e0160051, PUBLIC LIBRARY SCIENCE, Jul. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, The current subgenus Drosophila (the traditional immigrans-tripunctata radiation) includes major elements of temperate drosophilid faunas in the northern hemisphere. Despite previous molecular phylogenetic analyses, the phylogeny of the subgenus Drosophila has not fully been resolved: the resulting trees have more or less varied in topology. One possible factor for such ambiguous results is taxon-sampling that has been biased towards New World species in previous studies. In this study, taxon sampling was balanced between Old and New World species, and phylogenetic relationships among 45 ingroup species selected from ten core species groups of the subgenus Drosophila were analyzed using nucleotide sequences of three nuclear and two mitochondrial genes. Based on the resulting phylogenetic tree, ancestral distributions and divergence times were estimated for each clade to test Throckmorton's hypothesis that there was a primary, early-Oligocene disjunction of tropical faunas and a subsequent mid-Miocene disjunction of temperate faunas between the Old and the New Worlds that occurred in parallel in separate lineages of the Drosophilidae. Our results substantially support Throckmorton's hypothesis of ancestral migrations via the Bering Land Bridge mainly from the Old to the New World, and subsequent vicariant divergence of descendants between the two Worlds occurred in parallel among different lineages of the subgenus Drosophila. However, our results also indicate that these events took place multiple times over a wider time range than Throckmorton proposed, from the late Oligocene to the Pliocene.

Expansion of presoldier cuticle contributes to head elongation during soldier differentiation in termites
Yasuhiro Sugime, Kota Ogawa, Dai Watanabe, Hiroyuki Shimoji, Shigeyuki Koshikawa, Toru Miura
SCIENCE OF NATURE, 102, 11-12, 71, SPRINGER HEIDELBERG, Dec. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, In termites, the soldier caste possesses morphological features suitable for colony defence, despite some exceptions. Soldiers are differentiated via two moultings through a presoldier stage with dramatic morphogenesis. While a number of morphological modifications are known to occur during the presoldier moult, growth and morphogenesis seem to continue even after the moult. The present study, using the damp-wood termite Hodotermopsis sjostedti, carried out morphological and histological investigations on the developmental processes during the presoldier stage that is artificially induced by the application of a juvenile hormone analogue. Measurements of five body parameters indicated that head length significantly increased during the 14-day period after the presoldier moult, while it did not increase subsequently to the stationary moult (pseudergate moult as control). Histological observations also showed that the cuticular development played a role in the presoldier head elongation, suggesting that the soft and flexible presoldier cuticle contributed to the soldier morphogenesis in termites.

Enhancer modularity and the evolution of new traits
Shigeyuki Koshikawa
FLY, 9, 4, 155, 159, TAYLOR & FRANCIS INC, Oct. 2015, [Peer-reviewed], [Invited], [International Magazine]
English, Scientific journal, Animals have modular cis-regulatory regions in their genomes, and expression of a single gene is often regulated by multiple enhancers residing in such a region. In the laboratory, and also in natural populations, loss of an enhancer can result in a loss of gene expression. Although only a few examples have been well characterized to date, some studies have suggested that an evolutionary gain of a new enhancer function can establish a new gene expression domain. Our recent study showed that Drosophila guttifera has more enhancers and additional expression domains of the wingless gene during the pupal stage, compared to D. melanogaster, and that these new features appear to have evolved in the ancestral lineage leading to D. guttifera.(1) Gain of a new expression domain of a developmental regulatory gene (toolkit gene), such as wingless, can cause co-option of the expression of its downstream genes to the new domain, resulting in duplication of a preexisting structure at this new body position. Recently, with the advancement of evodevo studies, we have learned that the developmental regulatory systems are strikingly similar across various animal taxa, in spite of the great diversity of the animals' morphology. Even behind "new" traits, cooptions of essential developmental genes from known systems are very common. We previously provided concrete evidence of gains of enhancer activities of a developmental regulatory gene underlying gains of new traits.(1) Broad occurrence of this scenario is testable and should be validated in the future.

Gain of cis-regulatory activities underlies novel domains of wingless gene expression in Drosophila
Shigeyuki Koshikawa, Matt W. Giorgianni, Kathy Vaccaro, Victoria A. Kassner, John H. Yoder, Thomas Werner, Sean B. Carroll
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112, 24, 7524, 7529, NATL ACAD SCIENCES, Jun. 2015, [Peer-reviewed], [Lead author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Changes in gene expression during animal development are largely responsible for the evolution of morphological diversity. However, the genetic and molecular mechanisms responsible for the origins of new gene-expression domains have been difficult to elucidate. Here, we sought to identify molecular events underlying the origins of three novel features of wingless (wg) gene expression that are associated with distinct pigmentation patterns in Drosophila guttifera. We compared the activity of cis-regulatory sequences (enhancers) across the wg locus in D. guttifera and Drosophila melanogaster and found strong functional conservation among the enhancers that control similar patterns of wg expression in larval imaginal discs that are essential for appendage development. For pupal tissues, however, we found three novel wg enhancer activities in D. guttifera associated with novel domains of wg expression, including two enhancers located surprisingly far away in an intron of the distant Wnt10 gene. Detailed analysis of one enhancer (the vein-tip enhancer) revealed that it overlapped with a region controlling wg expression in wing crossveins (crossvein enhancer) in D. guttifera and other species. Our results indicate that one novel domain of wg expression in D. guttifera wings evolved by co-opting pre-existing regulatory sequences governing gene activity in the developing wing. We suggest that the modification of existing enhancers is a common path to the evolution of new gene-expression domains and enhancers.

Sexually dimorphic body color is regulated by sex-specific expression of yellow gene in ponerine ant, Diacamma sp.
Satoshi Miyazaki, Yasukazu Okada, Hitoshi Miyakawa, Gaku Tokuda, Richard Cornette, Shigeyuki Koshikawa, Kiyoto Maekawa, Toru Miura
PloS one, 9, 3, e92875, 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal, Most hymenopteran species exhibit conspicuous sexual dimorphism due to ecological differences between the sexes. As hymenopteran genomes, under the haplodiploid genetic system, exhibit quantitative differences between sexes while remaining qualitatively identical, sexual phenotypes are assumed to be expressed through sex-specific gene usage. In the present study, the molecular basis for expression of sexual dimorphism in a queenless ant, Diacamma sp., which exhibits a distinct color dimorphism, was examined. Worker females of the species appear bluish-black, while winged males exhibit a yellowish-brown body color. Initially, observations of the pigmentation processes during pupal development revealed that black pigmentation was present in female pupae but not in males, suggesting that sex-specific melanin synthesis was responsible for the observed color dimorphism. Therefore, five orthologs of the genes involved in the insect melanin synthesis (yellow, ebony, tan, pale and dopa decarboxylase) were subcloned and their spatiotemporal expression patterns were examined using real-time quantitative RT-PCR. Of the genes examined, yellow, which plays a role in black melanin synthesis in insects, was expressed at higher levels in females than in males throughout the entire body during the pupal stage. RNA interference of yellow was then carried out in order to determine the gene function, and produced females with a more yellowish, brighter body color similar to that of males. It was concluded that transcriptional regulation of yellow was responsible for the sexual color dimorphism observed in this species.

Soldier morphogenesis in the damp-wood termite is regulated by the insulin signaling pathway.
Akiko Hattori, Yasuhiro Sugime, Chifune Sasa, Hitoshi Miyakawa, Yuki Ishikawa, Satoshi Miyazaki, Yasukazu Okada, Richard Cornette, Laura Corley Lavine, Douglas J Emlen, Shigeyuki Koshikawa, Toru Miura
Journal of experimental zoology. Part B, Molecular and developmental evolution, 320, 5, 295, 306, 5, Jul. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal, Eusocial insects exhibit various morphological castes associated with the division of labor within a colony. Termite soldiers possess defensive traits including mandibles that are greatly exaggerated and enlarged, as compared to termite reproductives and workers. The enlarged mandibles of soldiers are known to result from dynamic morphogenesis during soldier differentiation that can be induced by juvenile hormone and its analogs. However, the detailed developmental mechanisms still remain unresolved. Because the insulin/insulin-like growth factor signaling (IIS) pathway has been shown to regulate the relative sizes of organs (i.e., allometry) in other insects, we examined the expression profiles of major IIS factors in the damp-wood termite Hodotermopsis sjostedti, during soldier differentiation. The relative expression patterns of orthologs for termite InR (HsjInR), PKB/Akt (HsjPKB/Akt), and FOXO (HsjFOXO) suggest that HsjInR and HsjPKB/Akt were up-regulated in the period of elongation of mandibles during soldier development. In situ hybridization showed that HsjInR was strongly expressed in the mandibular epithelial tissues, and RNA interference (RNAi) for HsjInR disrupted soldier-specific morphogenesis including mandibular elongation. These results suggest that signaling through the IIS pathway is required for soldier-specific morphogenesis. In addition, up-regulation of the IIS pathway in other body tissues occurred at earlier stages of development, indicating that there is tissue-specific IIS regulation. Because the IIS pathway is generally thought to act upstream of JH in insects, our results suggest the damp-wood termite may have evolved a novel feedback loop between JH and IIS that enables social interactions, rather than nutrition, to regulate caste determination.

Differential gene expression in response to juvenile hormone analog treatment in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Archotermopsidae)
Richard Cornette, Yoshinobu Hayashi, Shigeyuki Koshikawa, Toru Miura
JOURNAL OF INSECT PHYSIOLOGY, 59, 4, 509, 518, PERGAMON-ELSEVIER SCIENCE LTD, Apr. 2013, [Peer-reviewed]
English, Scientific journal, Termite societies are characterized by a highly organized division of labor among conspicuous castes, groups of individuals with various morphological specializations. Termite caste differentiation is under control of juvenile hormone OH), but the molecular mechanism underlying the response to JH and early events triggering caste differentiation are still poorly understood. In order to profile candidate gene expression during early soldier caste differentiation of the damp-wood termite, Hodotermopsis sjostedti, we treated pseudergates (workers) with a juvenile hormone analog (JHA) to induce soldier caste differentiation. We then used Suppressive Subtractive Hybridization to create two cDNA libraries enriched for transcripts that were either up- or downregulated at 24 h after treatment. Finally, we used quantitative PCR to confirm temporal expression patterns. Hexamerins represent a large proportion of the genes upregulated following JHA treatment and have an expression pattern that shows roughly an inverse correlation to intrinsic JH titers. This data is consistent with the role of a JH "sink", which was demonstrated for hexamerins in another termite, Reticulitermes flavipes. A putative nuclear protein was also upregulated a few hours after JHA treatment, which suggests a role in the early response to JH and subsequent regulation of transcriptional events associated with soldier caste differentiation. Some digestive enzymes, such as endogenous beta-endoglucanase and chymotrypsin, as well as a protein associated to digestion were identified among genes downregulated after JHA treatment. This suggests that JH may directly influence the pseudergate-specific digestive system. (c) 2013 Elsevier Ltd. All rights reserved.

Screening of upregulated genes induced by high density in the vetch aphid Megoura crassicauda.
Asano Ishikawa, Yuki Ishikawa, Yasukazu Okada, Satoshi Miyazaki, Hitoshi Miyakawa, Shigeyuki Koshikawa, Jennifer A Brisson, Toru Miura
Journal of experimental zoology. Part A, Ecological genetics and physiology, 317, 3, 194, 203, 3, Mar. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal, Aphids exhibit several polyphenisms in which discontinuous, alternative phenotypes are produced depending on environmental conditions. One representative example is the wing polyphenism, where winged and wingless females are produced through parthenogenesis. Previous work has shown that, in some aphid species, the density condition sensed by the mother aphid determines the developmental fate of embryos in her ovary, with high densities leading to winged progeny and low densities to wingless progeny. However, little is known about the molecular and physiological mechanisms underlying the wing polyphenism. To identify genes involved in the wing-morph determination in the vetch aphid, Megoura crassicauda, we compared maternal and embryonic transcripts between high- and low-density conditions using differential display, followed by quantitative real-time PCR (qRT-PCR). Under the high-density condition, two genes (Uba1 and Naca) were found to be upregulated in maternal tissues without ovaries, while one gene (ClpP) was upregulated in ovaries containing embryos. Uba1 and Naca encode factors that function in protein modification or transcriptional/translational regulation, respectively. In addition to differential display, candidate gene approaches focusing on morphogenetic and endocrine genes, i.e., wg, dpp, ap, hh, InR, IRS, Foxo, EcR, and USP, were also carried out. We found that wg was upregulated in maternal tissues under the high-density condition. The identified genes from both approaches are candidates for further study of their involvement in the transduction of density signals in mother aphids and/or the initial process of wing differentiation in embryos.

Juvenile Hormone Regulates Extreme Mandible Growth in Male Stag Beetles
Hiroki Gotoh, Richard Cornette, Shigeyuki Koshikawa, Yasukazu Okada, Laura Corley Lavine, Douglas J. Emlen, Toru Miura
PLOS ONE, 6, 6, e21139, PUBLIC LIBRARY SCIENCE, Jun. 2011, [Peer-reviewed]
English, Scientific journal, The morphological diversity of insects is one of the most striking phenomena in biology. Evolutionary modifications to the relative sizes of body parts, including the evolution of traits with exaggerated proportions, are responsible for a vast range of body forms. Remarkable examples of an insect trait with exaggerated proportions are the mandibular weapons of stag beetles. Male stag beetles possess extremely enlarged mandibles which they use in combat with rival males over females. As with other sexually selected traits, stag beetle mandibles vary widely in size among males, and this variable growth results from differential larval nutrition. However, the mechanisms responsible for coupling nutrition with growth of stag beetle mandibles (or indeed any insect structure) remain largely unknown. Here, we demonstrate that during the development of male stag beetles (Cyclommatus metallifer), juvenile hormone (JH) titers are correlated with the extreme growth of an exaggerated weapon of sexual selection. We then investigate the putative role of JH in the development of the nutritionally-dependent, phenotypically plastic mandibles, by increasing hemolymph titers of JH with application of the JH analog fenoxycarb during larval and prepupal developmental periods. Increased JH signaling during the early prepupal period increased the proportional size of body parts, and this was especially pronounced in male mandibles, enhancing the exaggerated size of this trait. The direction of this response is consistent with the measured JH titers during this same period. Combined, our results support a role for JH in the nutrition-dependent regulation of extreme mandible growth in this species. In addition, they illuminate mechanisms underlying the evolution of trait proportion, the most salient feature of the evolutionary diversification of the insects.

Identification of a reproductive-specific, putative lipid transport protein gene in a queenless ponerine ant Diacamma sp.
Yasukazu Okada, Satoshi Miyazaki, Shigeyuki Koshikawa, Richard Cornette, Kiyoto Maekawa, Kazuki Tsuji, Toru Miura
Naturwissenschaften, 97, 11, 971, 9, Nov. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal, Of the various characteristics of social insects, communication for reproductive differentiation is one of the most important and basic social interactions among colony members. To elucidate the molecular basis underlying this process, genes responsible for reproductive differentiation in Diacamma were screened using fluorescent differential display. Differential display, together with real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), revealed that a gene belonging to the family of cellular retinaldehyde-binding proteins was specifically expressed in the epidermis of the head, legs, and thorax in reproductives. The deduced protein sequence in the coding region, obtained by rapid amplification of cDNA ends (RACE)-PCR, was found to include cellular retinaldehyde-binding domain (CRAL-TRIO domain), suggesting that DiaCRALDCP functions in transportation of lipids, such as cuticular hydrocarbons. DiaCRALDCP transcript levels immediately decreased 1 day after the gemma mutilation, suggesting that DiaCRALDCP is involved in the physiological changes provoked by the behavioral regulation. Considering these results, the social functions of DiaCRALDCP in Diacamma are discussed.

Evolution of color and pattern in Drosophila
Shigeyuki Koshikawa
Biological Science, 62, 1, 19, 29, Sep. 2010, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
Japanese, Scientific journal, In Japanese

The homolog of Ciboulot in the termite (Hodotermopsis sjostedti): a multimeric beta-thymosin involved in soldier-specific morphogenesis
Shigeyuki Koshikawa, Richard Cornette, Tadao Matsumoto, Toru Miura
BMC DEVELOPMENTAL BIOLOGY, 10, 63, BIOMED CENTRAL LTD, Jun. 2010, [Peer-reviewed]
English, Scientific journal, Background: Caste differentiation in social insects is a type of polyphenism that enables division of labor among members of a colony. This elaborate social integration has attracted broad interest, although little is known about its regulatory mechanisms, especially in Isoptera (termites). In this study, we analyzed soldier differentiation in the damp-wood termite Hodotermopsis sjostedti, focusing on a possible effector gene for caste development. The gene for an actin-binding protein, HsjCib, which shows a high level of expression in developing mandibles during soldier differentiation, is characterized in detail.
Results: To examine the HsjCib gene, full-length cDNAs were obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) and sequencing. Multiple isoforms were identified, and on the basis of the results of northern and Southern hybridization analyses, these isoforms were considered to be transcriptional variants from a single gene. On the basis of their sequence similarity to homologous genes of other organisms, functions in actin assembly were assumed to be different among isoforms. Expression analysis revealed high expression in the head during soldier differentiation, which was consistent with their allometric growth. Although isoform expression was observed in various tissues, different expression levels were observed among tissues, suggesting the possibility of tissue-specific morphogenetic regulation by HsjCib isoforms.
Conclusion: This study revealed the characteristics and dynamics of the HsjCib gene during soldier differentiation as a potential representative of downstream effector genes in caste-specific morphogenesis. From the expression patterns observed, this gene is considered to be involved in cephalic morphogenesis and neural reorganization, resulting in the establishment of caste-specific morphology and behavior.

Gene expression changes during caste-specific neuronal development in the damp-wood termite Hodotermopsis sjostedti.
Yuki Ishikawa, Yasukazu Okada, Asano Ishikawa, Hitoshi Miyakawa, Shigeyuki Koshikawa, Toru Miura
BMC genomics, 11, 314, 314, 20 May 2010, [Peer-reviewed], [International Magazine]
English, BACKGROUND: One of the key characters of social insects is the division of labor, in which different tasks are allocated to various castes. In termites, one of the representative groups of social insects, morphological differences as well as behavioral differences can be recognized among castes. However, very little is known about the neuronal and molecular bases of caste differentiation and caste-specific behavior. In almost all termite species, soldiers play defensive roles in their colonies, and their morphology and behavior are largely different from workers (or pseudergates). Therefore, we predicted that some genes linked to defensive behavior and/or those required for neuronal changes are differentially expressed between workers and soldiers, or during the soldier differentiation, respectively. RESULTS: Using the brain and suboesophageal ganglion (SOG) of the damp-wood termite Hodotermopsis sjostedti, we first screened genes specifically expressed in soldiers or during soldier differentiation by the differential display method, followed by quantitative real-time polymerase chain reaction. No distinctive differences in expression patterns were detected between pseudergates and soldiers. In the course of soldier differentiation, however, five genes were found to be up-regulated in brain and/or SOG: 14-3-3 epsilon, fibrillin2, beta-tubulin, ciboulot, and a hypothetical protein containing a SAP motif. Some of these genes are thought to be associated with cytoskeletal structure or motor-associated proteins in neuronal tissues. CONCLUSION: The identified five genes could be involved in soldier-specific neuronal modifications, resulting in defensive behaviors in termite soldiers. The temporal expression patterns of these genes were consistent with the neuronal changes during soldier differentiation, suggesting that molecular machineries, in which the identified factors would participate, play important roles in behavioral differentiation of termite soldiers.

Generation of a novel wing colour pattern by the Wingless morphogen
Thomas Werner, Shigeyuki Koshikawa, Thomas M. Williams, Sean B. Carroll
NATURE, 464, 7292, 1143, 1148, NATURE PUBLISHING GROUP, Apr. 2010, [Peer-reviewed], [Lead author]
English, Scientific journal, The complex, geometric colour patterns of many animal bodies have important roles in behaviour and ecology. The generation of certain patterns has been the subject of considerable theoretical exploration, however, very little is known about the actual mechanisms underlying colour pattern formation or evolution. Here we have investigated the generation and evolution of the complex, spotted wing pattern of Drosophila guttifera. We show that wing spots are induced by the Wingless morphogen, which is expressed at many discrete sites that are specified by pre-existing positional information that governs the development of wing structures. Furthermore, we demonstrate that the elaborate spot pattern evolved from simpler schemes by co-option of Wingless expression at new sites. This example of a complex design developing and evolving by the layering of new patterns on pre-patterns is likely to be a general theme in other animals.

Gene up-regulation in response to predator kairomones in the water flea, Daphnia pulex
Hitoshi Miyakawa, Maki Imai, Naoki Sugimoto, Yuki Ishikawa, Asano Ishikawa, Hidehiko Ishigaki, Yasukazu Okada, Satoshi Miyazaki, Shigeyuki Koshikawa, Richard Cornette, Toru Miura
BMC DEVELOPMENTAL BIOLOGY, 10, 45, BMC, Apr. 2010, [Peer-reviewed]
English, Scientific journal, Background: Numerous cases of predator-induced polyphenisms, in which alternate phenotypes are produced in response to extrinsic stimuli, have been reported in aquatic taxa to date. The genus Daphnia (Branchiopoda, Cladocera) provides a model experimental system for the study of the developmental mechanisms and evolutionary processes associated with predator-induced polyphenisms. In D. pulex, juveniles form neckteeth in response to predatory kairomones released by Chaoborus larvae (Insecta, Diptera).Results: Previous studies suggest that the timing of the sensitivity to kairomones in D. pulex can generally be divided into the embryonic and postembryonic developmental periods. We therefore examined which of the genes in the embryonic and first-instar juvenile stages exhibit different expression levels in the presence or absence of predator kairomones. Employing a candidate gene approach and identifying differentially-expressed genes revealed that the morphogenetic factors, Hox3, extradenticle and escargot, were up-regulated by kairomones in the postembryonic stage and may potentially be responsible for defense morph formation. In addition, the juvenile hormone pathway genes, JHAMT and Met, and the insulin signaling pathway genes, InR and IRS-1, were up-regulated in the first-instar stage. It is well known that these hormonal pathways are involved in physiological regulation following morphogenesis in many insect species. During the embryonic stage when morphotypes were determined, one of the novel genes identified by differential display was up-regulated, suggesting that this gene may be related to morphotype determination. Biological functions of the up-regulated genes are discussed in the context of defense morph formation.Conclusions: It is suggested that, following the reception of kairomone signals, the identified genes are involved in a series of defensive phenotypic alterations and the production of a defensive phenotype.

Genome size of Pachypsylla venusta (Hemiptera: Psyllidae) and the ploidy of its bacteriocyte, the symbiotic host cell that harbors intracellular mutualistic bacteria with the smallest cellular genome
A. Nakabachi, S. Koshikawa, T. Miura, S. Miyagishima
BULLETIN OF ENTOMOLOGICAL RESEARCH, 100, 1, 27, 33, CAMBRIDGE UNIV PRESS, Feb. 2010, [Peer-reviewed]
English, Scientific journal, Psyllids harbor the primary symbiont, Carsonella ruddii (gamma-Proteobacteria), within the cytoplasm of specialized cells called bacteriocytes. Carsonella has the smallest known cellular genome (160kb), lacking numerous genes that appear to be essential for bacterial life. This raises the question regarding the genetic mechanisms of the host which supports the survival of Carsonella. Our preceding analyses have indicated that some of the genes that are encoded in the psyllid genome and which are highly expressed in the bacteriocyte are of bacterial origin. This implies that psyllids acquired genes from bacteria by lateral gene transfer (LGT) and are using these genes to maintain the primary symbiont, Carsonella. To reveal the complete Picture of LGT from symbiotic bacteria to the genome of psyllids, whole genome analysis of psyllid is essential. In order to assess the feasibility of whole genome analysis of the host psyllid, the genome size of the hackberry petiole gall psyllid, Pachypsylla venusta, was estimated. Feulgen image analysis densitometry and flow cytometry demonstrated that the haploid genome size of P. venusta is 0.74pg (724Mb), verifying the feasibility of whole genome analysis. Feulgen image analysis densitometry further revealed that bacteriocytes of P. venusta are invariably 16-ploid. This higher ploidy may be essential to facilitate the symbiotic relationship with bacteria, as it appears to be a feature common to insect bacteriocytes. These results provide a foundation for genomics-based research into host-symbiont interactions.

Histology of the hormone-producing glands in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Termopsidae): a focus on soldier differentiation
Cornette R, Koshikawa S, Miura T
Insectes Sociaux, 55, 4, 407, 416, Sep. 2008, [Peer-reviewed]
English, Scientific journal

Genome size of termites (Insecta, Dictyoptera, Isoptera) and wood roaches (Insecta, Dictyoptera, Cryptocercidae)
Shigeyuki Koshikawa, Satoshi Miyazaki, Richard Cornette, Tadao Matsumoto, Toru Miura
NATURWISSENSCHAFTEN, 95, 9, 859, 867, SPRINGER, Sep. 2008, [Peer-reviewed]
English, Scientific journal, The evolution of genome size has been discussed in relation to the evolution of various biological traits. In the present study, the genome sizes of 22 dictyopteran species were estimated by Feulgen image analysis densitometry and 6-diamidino-2-phenylindole (DAPI)-based flow cytometry. The haploid genome sizes (C-values) of termites (Isoptera) ranged from 0.58 to 1.90 pg, and those of Cryptocercus wood roaches (Cryptocercidae) were 1.16 to 1.32 pg. Compared to known values of other cockroaches (Blattaria) and mantids (Mantodea), these values are low. A relatively small genome size appears to be a (syn)apomorphy of Isoptera + Cryptocercus, together with their sociality. In some phylogenetic groups, genome size evolution is thought to be influenced by selective pressure on a particular trait, such as cell size or rate of development. The present results raise the possibility that genome size is influenced by selective pressures on traits associated with the evolution of sociality.

Compound Eye Development During Caste Differentiation in the Termite Reticulitermes speratus (Isoptera: Rhinotermitidae)
Kiyoto Maekawa, Sayaka Mizuno, Shigeyuki Koshikawa, Toru Miura
ZOOLOGICAL SCIENCE, 25, 7, 699, 705, ZOOLOGICAL SOC JAPAN, Jul. 2008, [Peer-reviewed]
English, Scientific journal, We morphologically examined postembryonic compound eye development in Reticulitermes speratus (Kolbe) to understand developmental regulation during caste differentiation. The eye primordia were shown to exist from the larval stage. The number of ommatidia and compound eye size greatly increased over the course of imaginal development. Nymphoids (second-form reproductives) possessed a developed compound eye structure on the surface of the cuticle and thick optic nerves, but individual ommatidia were not clearly discriminated. However, in the line of apterous workers and soldiers, although the outer rim of the eye was observed from second-stage workers, there were few morphological differences among instars, including ergatoids (third-form reproductives). Both nymphoids and ergatoids are slightly physogastric and have highly developed reproductive organs. These results suggest that eye development in the apterous line could be strongly arrested and that there is a weak developmental correlation between the eyes and reproductive organs in R. speratus.

Juvenile hormone titers and caste differentiation in the damp-wood termite Hodotermopsis sjostedri (Isoptera, Termopsidae)
Richard Cornette, Hiroki Gotoh, Shigeyuki Koshikawa, Toru Miura
JOURNAL OF INSECT PHYSIOLOGY, 54, 6, 922, 930, PERGAMON-ELSEVIER SCIENCE LTD, Jun. 2008, [Peer-reviewed]
English, Scientific journal, Termites are social insects, presenting morphologically distinct castes, performing specific tasks in the colony. The developmental processes underlying caste differentiation are mainly controlled by juvenile hormone (JH). Although many fragmentary data support this fact, there was no comparative work on JH titers during the caste differentiation processes. In this study, JH titer variation was investigated using a liquid chromatography-mass spectrometry (LC-MS) quantification method in all castes of the Japanese damp-wood termite Hodotermopsis sjostedti, especially focusing on the soldier caste differentiation pathway, which was induced by treatment with a JH analog. Hemolymph JH titers fluctuated between 20 and 720 pg/mu l. A peak of JH was observed during molting events for the pseudergate stationary molt and presoldier differentiation, but this peak was absent prior to the imaginal molt. Soldier caste differentiation was generally associated with high JH titers and nymph to alate differentiation with low JH titers. However, JH titer rose in females during alate maturation, probably in relation to vitellogenesis. In comparison, JH titer was surprisingly low in neotenics. On the basis of these results in both natural and artificial conditions, the current model for JH action on termite caste differentiation is discussed and re-appraised. (C) 2008 Elsevier Ltd. All rights reserved.

Effects of precocenes on the corpora allata and the JH titer in the damp-wood termite Hodotermopsis sjostedti (Isoptera : Termopsidae)
Hiroki Gotoh, Richard Cornette, Shigeyuki Koshikawa, Toru Miura
SOCIOBIOLOGY, 52, 2, 345, 356, CALIFORNIA STATE UNIV, 2008, [Peer-reviewed]
English, Scientific journal, Juvenile hormone (JH) is an insect hormone that is responsible for the control of a variety of physiological and developmental states. In termites' JH has long been considered to play an important role in caste differentiation, although the detailed mechanisms underlying the actions of the hormone are not well understood. In this study, we applied three types of precocenes, anti-JH agents that destroy the corpora allata (CA), to pseudergates (workers) of the damp-wood termite Hodotermopsis sjostedti, and evaluated the effects on the CA volume and JH titer after the treatments. Results showed degeneration of the CA at day 14 in the precocene-II-treated group, which was not observed in the control group. Conversely, no significant difference in the JH titer was apparent between precocenc-II-treated groups and their corresponding controls. These results indicate that CA degeneration is not always correlated with a decrease in JH titer. Precocenes are thus not effective as anti-JH agents in the focal termite species. These findings indicate that simultaneous examination of JH titer and CA size are important considerations for evaluating the effects of anti-JH agents.

Caste differentiation and gene expression in termites: perspective for sociogenomics
KOSHIKAWA SHIGEYUKI, MIURA TORU
Japanese Journal of Ecology, 57, 1, 82, 88, Mar. 2007, [Peer-reviewed], [Invited]
Japanese, Scientific journal, In Japanese

Differences in mechanosensory hairs among castes of the damp-wood termite Hodotermopsis sjostedti (Isoptera: Termitidae)　　　　　　　　　　　　　　　
Ishikawa Y, Koshikawa S, Miura T
Sociobiology, 50, 3, 895, 907, 2007, [Peer-reviewed]
English, Scientific journal

Caste-specific cytochrome P450 in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Termopsidae)
R Cornette, S Koshikawa, M Hojo, T Matsumoto, T Miura
INSECT MOLECULAR BIOLOGY, 15, 2, 235, 244, BLACKWELL PUBLISHING, Apr. 2006, [Peer-reviewed]
English, Scientific journal, Termites are eusocial insects with a well-defined caste system, which is an example of polyphenism. This polyphenism is based on hormonally controlled differential gene expression. In the damp-wood termite Hodotermopsis sjostedti, we induced differentiation into the soldier caste by using juvenile hormone analogue treatment. We then investigated specific gene expression, which appeared during the hormonal response and triggered caste differentiation, using fluorescent differential display. A candidate cDNA sequence with similarity to cytochromes P450, CYP6AM1, was characterized and its transcript shown to be repressed between 1 and 3 days after hormone treatment. CYP6AM1 was specifically expressed in the fat body of pseudergates and soldiers. The putative function of this P450 is discussed with respect to the caste differentiation system.

Identification of soldier-specific genes in the nasute termite Nasutitermes takasagoensis (Isoptera: Termitidae)
HOJO MASARU, KOSHIKAWA SHIGEYUKI, CORNETTE RICHARD, MATSUMOTO TADAO, MIURA TORU
Entomol Sci, 8, 4, 379, 387, Dec. 2005
English, Scientific journal

Mandibular morphogenesis and gene expression during soldier differentiation in termites
Shigeyuki Koshikawa, Tadao Matsumoto, Toru Miura
The Nature and Insect, 40, 11, 17, 21, Nov. 2005, [Invited], [Lead author, Corresponding author]
Japanese, Scientific journal, In Japanese

Caste-specific morphogenesis and gene expression during soldier differentiation in the damp -wood termite Hodotermopsis sjostedti（Isoptera, Termopsidae）　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
Graduate School of Science, The University of Tokyo, Mar. 2005
English, Doctoral thesis, 博理第4723号

Screening of genes expressed in developing mandibles during soldier differentiation in the termite Hodotermopsis sjostedti
S Koshikawa, R Cornette, M Hojo, K Maekawa, T Matsumoto, T Miura
FEBS LETTERS, 579, 6, 1365, 1370, ELSEVIER SCIENCE BV, Feb. 2005, [Peer-reviewed], [Lead author]
English, Scientific journal, We investigated the morphological changes accompanying soldier differentiation in the damp-wood termite Hodotermopsis sjostediti. Genes expressed in the developing mandibles, which undergo the most remarkable morphological changes during soldier differentiation, were screened using fluorescent differential display. Database searches for sequence similarities were conducted and the relative expression levels were then quantified by real-time polymerase chain reaction. Among the identified candidate genes, 12 genes were upregulated during soldier differentiation. These included genes for cuticle proteins, nucleic acid binding proteins, ribosomal proteins and actin-binding protein, which were inferred to be involved in caste-sped c morphogenesis in termites. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Comparative studies on alate wing formation in two related species of rotten-wood termites: Hodotermopsis sjostedti and Zootermopsis nevadensis (Isoptera, Termopsidae)
T Miura, S Koshikawa, M Machida, T Matsumoto
INSECTES SOCIAUX, 51, 3, 247, 252, BIRKHAUSER VERLAG AG, Aug. 2004, [Peer-reviewed]
English, Scientific journal, Termite colonies are comprised of several types of castes that differentiate throughout postembryonic development. In termopsid termites (family Termopsidae), alates are normally differentiated from apterous instars by two nymphal instar stages and three moulting events. Here, we report that of the rotten-wood termite Hodotermopsis sjostedti. There is only a single nymphal instar between the pseudergate and alate stages. During the annual alate production season in June/July, we observed some nymphs with small wing buds which were dorsally swollen. Those nymphal individuals subsequently moulted into alates through only a single moult. We examined their histology and internal morphology and observed that the folding pattern of the newly formed wings was very different from that seen in second stage nymphs of the closely related species Zootermopsis nevadensis. The newly formed wings of H. sjostedti are formed inside the relatively smaller wing buds and therefore must be folded in a complicated manner. Our observations revealed that the tips of the folded wings were elongated and bent, such that they overlapped in the median plane. We suggest that heterochronic evolutionary change accounts for the compression of nymphal development into a single instar. We also suggest that this probably occurred at either the individual or colony level in this species.

Soldier-like intercastes in the rotten-wood termite Hodotermopsis sjostedti (Isoptera : Termopsidae)
S Koshikawa, T Matsumoto, T Miura
ZOOLOGICAL SCIENCE, 21, 5, 583, 588, ZOOLOGICAL SOC JAPAN, May 2004, [Peer-reviewed]
English, Scientific journal, All termite species possess a distinct sterile-soldier caste in their colonies, although reproductive soldiers, with soldier characteristics and reproductive ability, have been reported from several species of the family Termopsidae. Such intercastes have been considered the primitive-soldier caste, and based on this many researchers have discussed the evolutionary origin of termite soldiers. We investigated whether such soldier-reproductive intercastes also exist in the Japanese rotten-wood termite Hodotermopsis sjostedti. Abnormal individuals with soldier-like characteristics were found and designated as soldier-like intercastes, which appeared to have both soldier and reproductive characteristics. Based on our morphometric analyses and histological examinations, we suggest that the developmental origin of this intercaste is a pseudergate, nymph or sixth-instar larva. In addition, the intercaste was found to have relatively well-developed gonads, although mature oocytes and spermatozoa were not found in female ovaries and male seminal vesicles, respectively. We conclude that the soldier-like intercaste of H. sjostedti does not have reproductive ability, which is different from all other known soldier-like intercastes in Termopsidae.

Developmental pathways and plasticity of neuter castes in Nasutitermes takasagoensis (Isoptera : Termitidae)
MU Hojo, S Koshikawa, T Matsumoto, T Miura
SOCIOBIOLOGY, 44, 2, 433, 441, CALIF STATE UNIV, 2004, [Peer-reviewed]
English, Scientific journal, Caste developmental pathways vary among termite species. We investigated the caste developmental patterns of Nasutitermes takasagoensis, based on morphometric data. These patterns appear to be identical to those of other Nasutitermes species. In addition, a nymph-presoldier intercaste was found during the study. We analyzed the detailed morphology of the intercaste and discuss the developmental plasticity of the caste differentiation in this nasute termite.

Winged presoldiers induced by a juvenile hormone analog in Zootermopsis nevadensis: Implications for plasticity and evolution of caste differentiation in termites
T Miura, S Koshikawa, T Matsumoto
JOURNAL OF MORPHOLOGY, 257, 1, 22, 32, WILEY-LISS, Jul. 2003, [Peer-reviewed]
English, Scientific journal, To elucidate the switching mechanism of caste differentiation in termites and to examine the possible induction of soldier-reproductive intercastes experimentally, we investigated the effects of juvenile hormone on the morphologies of soldier caste by applying a juvenile hormone analog (JHA) to nymphs of the damp-wood termite Zootermopsis nevadensis (Isoptera : Termopsidae). JHA treatment for about 2 weeks induced a variety of intermediate castes, showing both alate and soldier morphological features. The principal component analysis (PCA) of those morphological characters showed that those intercastes were a deviation from the developmental line into alates to soldier differentiation, which is known to be triggered by juvenile hormone. Detailed morphological examination of the compound eyes, wing joint, and mandibles showed that those intercastes expressed soldier features, although they had started to develop alate characteristics. The morphology of the resultant intercastes seemed to be determined by the nymphal stage, at which JHA treatment was applied. The induced intercastes with exaggerated soldier-specific characteristics (e.g., mandibles) repressed alate-specific characteristics (e.g., wings), namely, the alate and soldier morphological characteristics in induced intercastes show opposite responses against the application of JHA. On the other hand, ovarian development was not suppressed by the JHA application, even in the soldier-like individuals. Naturally differentiated presoldiers also possessed developed ovarioles, although ovaries of mature soldiers were degenerated. Our results suggest that the juvenile hormone plays complicated roles in the expression of caste morphologies and ovarian development in termites. (C) 2003 Wiley-Liss, Inc.

Mandibular morphogenesis during soldier differentiation in the damp-wood termite Hodotermopsis sjoestedti (Isoptera : Termopsidae)
S Koshikawa, T Matsumoto, T Miura
NATURWISSENSCHAFTEN, 90, 4, 180, 184, SPRINGER-VERLAG, Apr. 2003, [Peer-reviewed]
English, Scientific journal, The conspicuous morphogenesis during termite soldier differentiation is one of the most remarkable examples of specialized caste development in social insects. To clarify the mechanism of morphological changes during soldier differentiation, mandibular morphogenesis prior to the presoldier moult was examined in the damp-wood termite Hodotermopsis sjoestedti. Using experimental induction of presoldier differentiation from pseudergates (7th or later instar larvae) by the application of juvenile hormone analogue (JHA), we compared mandibular morphogenesis in a presoldier moult (from pseudergate to presoldier) with that of a stationary moult (from pseudergate to pseudergate). Future presoldier mandibles were formed under the old mandibular cuticle of the pseudergates just prior to the presoldier moult. Multiple folds of both epidermis and new cuticle were observed in the developing mandible, and large concavities between teeth were formed on the mandibular surface. While in the stationary moult, the future mandibles were formed with a smooth surface. The process of the mandibular morphogenesis in soldier differentiation explains the allometry of soldier mandibles. The morphological differences in mouth parts between castes arise from a switching mechanism that triggers caste-specific mandibular epithelial growth during development.

社会性昆虫のカースト分化と幼若ホルモン
MIURA TORU, KOSHIKAWA SHIGEYUKI, MATSUMOTO TADAO
JSCE Newsletter, 104, 14, 18, Feb. 2002, [Peer-reviewed], [Invited]
Japanese, Scientific journal, In Japanese

Morphometric changes during soldier differentiation of the damp-wood termite Hodotermopsis japonica (Isoptera, Termopsidae)
S Koshikawa, T Matsumoto, T Miura
INSECTES SOCIAUX, 49, 3, 245, 250, BIRKHAUSER VERLAG AG, 2002, [Peer-reviewed]
English, Scientific journal, To clarify the allometric development of body parts accompanying soldier differentiation in termites, we measured 16 body parts of soldiers, presoldiers, pseudergates (workers), nymphs and larvae of the damp-wood termite Hodotermopsis japonica. Principal component analysis (PCA) was performed using these parameters, which revealed that differentiation into soldiers differed distinctly from development into adult (reproductive) individuals. In particular, the anterior body parts enlarged during development of soldiers. Similarly, elongation of the apical portion of both mandibles was noted during soldier differentiation. X-ray analysis of mandibles revealed sclerotization of the soldier mandibles after differentiation into terminal soldiers. These morphological changes during soldier differentiation are associated with changes in their roles within the colony. Through soldier differentiation, the morphology of this caste of termite becomes functionally suited for attacking predators, and unsuitable for feeding on wood using their mandibles. Based on these data, we suggest that there must be some morphogenetic factors leading caste specific morphology such as soldier mandibles.

Regressive molt in the Japanese damp-wood termite Hodotermopsis japonica (Isoptera : Termopsidae)
S Koshikawa, T Matsumoto, T Miura
SOCIOBIOLOGY, 38, 3A, 495, 500, CALIF STATE UNIV, 2001, [Peer-reviewed]
English, Scientific journal, Regressive molts from nymphs to pseudergates were observed in Hodotermopsis japonica in experimental groups composed of nymphs alone. This is the first study of the genus Hodotermopsis, and the regressive molt in this species was unique for Termopsidae because wing bud biting prior to the regressive molt was not observed. The thoracic morphologies of pseudergates derived from nymphs were different from those of normal pseudergates: the posterior margin of the metanotum was trapezoidal. It was suggested that the regressive molt occasionally occurs in this species, and is one of the features caused by the flexibility of caste differentiation in termites.

寄生性昆虫ナガカメネジレバネの宿主体内への侵入行動　　　　　　　　　　　　　　　
菅藤隼人, 越川滋行, 日本動物行動学会 第43回大会 帝京科学大学東京西キャンパス 映像発表 V09, 04 Nov. 2024, [Last author]
Japanese, Summary national conference

寄生性昆虫ナガカメネジレバネの寄生行動と生態　　　　　　　　　　　　　　　
菅藤隼人, 中瀬悠太, 越川滋行, 第2回北海道バイオ"Mix-up" ポスター発表, 25 Sep. 2024, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエの模様形成に寄与するWntシグナル遺伝子　　　　　　　　　　　　　　　
酒井颯汰, 古関将斗, 越川滋行, 第2回北海道バイオ"Mix-up" ポスター発表, 25 Sep. 2024, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエのwinglessのco-optionにおいて、それぞれのシス制御配列の変化はどのような働きをしたのだろうか？　　　　　　　　　　　　　　　
柄澤匠, 越川滋行, 第2回北海道バイオ"Mix-up" ポスター発表, 25 Sep. 2024, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエの翅におけるWntシグナル遺伝子の発現パターン　　　　　　　　　　　　　　　
酒井颯汰, 古関将斗, 越川滋行, コントラリアン生物学 - シンポジウム「集団における個とは？集団内多様性のダイナミクスを考える」ポスター発表, 20 Sep. 2024, [Last author]
Japanese, Summary national conference

How did each cis-regulatory change contribute to the evolution of the novel expression pattern in Drosophila guttifera?　　　　　　　　　　　　　　　
Takumi Karasawa, Shigeyuki Koshikawa, The 16th Japanese Drosophila Research Conference (JDRC16) ポスター発表, 17 Sep. 2024, [Last author]
English, Summary national conference

ショウジョウバエの水玉模様を形成する遺伝的基盤を解明するためのRNAi 技術の確立　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 日本動物学会第95回長崎大会（長崎大学文教キャンパス）口頭発表, 12 Sep. 2024, [Last author]
Japanese, Summary national conference

The life history of Blissoxenos esakii (Strepsiptera: Corioxenidae): superparasitism, first instar larva, and female adults　　　　　　　　　　　　　　　
Hayato Kanto, Yuta Nakase, Shigeyuki Koshikawa, ICE2024 (XXVII International Congress of Entomology, Kyoto), Poster, 29 Aug. 2024, [Last author]
English, Summary international conference

Searching for genes responsible for the formation of ectopic campaniform sensillum in Drosophila guttifera　　　　　　　　　　　　　　　
Fuka Hiramatsu, Shigeyuki Koshikawa, ICE2024 (XXVII International Congress of Entomology, Kyoto), Poster, 29 Aug. 2024, [Last author]
English, Summary international conference

Sequence level mechanism underlying the evolution of the unique wing pigmentation pattern of Drosophila guttifera　　　　　　　　　　　　　　　
Takumi Karasawa, Shigeyuki Koshikawa, ICE2024 (XXVII International Congress of Entomology, Kyoto), Symposium [8-3] Frontiers in Research on the Molecular Basis underlying the Diversity of Insect Color Patterns, 27 Aug. 2024, [Last author]
English, Summary international conference

Modification of the wing color pattern of Drosophila guttifera by developing the Gal4/UAS system　　　　　　　　　　　　　　　
Masato Koseki, Shigeyuki Koshikawa, ICE2024 (XXVII International Congress of Entomology, Kyoto), Poster, 26 Aug. 2024, [Last author]
English, Summary international conference

Genome sequencing revealed the pseudogenization of visual genes in trechine beetles living in caves　　　　　　　　　　　　　　　
Takuma Niida, Shigeyuki Koshikawa, ICE2024 (XXVII International Congress of Entomology, Kyoto), Symposium [2-1] Insect Bio Digital Transformation (Insect BioDX), 26 Aug. 2024, [Last author]
English, Summary international conference

ミズタマショウジョウバエを用いて新しい模様の進化を塩基配列レベルで理解する　　　　　　　　　　　　　　　
柄澤匠, 越川滋行, 第26回日本進化学会神奈川大会（東海大学湘南キャンパス、平塚市）W4 多様なショウジョウバエから解明する進化のメカニズム, 22 Aug. 2024, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエにおける異所的な鐘状感覚子の形成に関わる遺伝子の探索　　　　　　　　　　　　　　　
平松楓佳, 越川滋行, 第26回日本進化学会神奈川大会（東海大学湘南キャンパス、平塚市）ポスター発表, 21 Aug. 2024, [Last author]
Japanese, Summary national conference

ショウジョウバエがもつ水玉模様の形成にモルフォゲンの発現は必要なのか？　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 第26回日本進化学会神奈川大会（東海大学湘南キャンパス、平塚市）口頭発表, 21 Aug. 2024, [Last author]
Japanese, Summary national conference

どのような配列の変化がミズタマショウジョウバエに模様をもたらしたのか？　　　　　　　　　　　　　　　
柄澤匠, 越川滋行, 日本昆虫学会第84回大会・第68回日本応用動物昆虫学会大会 合同大会（ポスター発表、仙台国際センター）, 29 Mar. 2024, [Last author]
Japanese, Summary national conference

洞窟性チビゴミムシ類における遺伝子の退化と多面発現の関係　　　　　　　　　　　　　　　
丹伊田拓磨, 越川滋行, 日本昆虫学会第84回大会・第68回日本応用動物昆虫学会大会 合同大会（ポスター発表、仙台国際センター）, 29 Mar. 2024, [Last author]
Japanese, Summary national conference

Is the wingless expression necessary to the wing color pattern formation of Drosophila guttifera?　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 日本昆虫学会第84回大会・第68回日本応用動物昆虫学会大会 合同大会（英語口頭発表、仙台国際センター）, 29 Mar. 2024, [Last author]
Best English Presentation Award, English, Summary national conference

ミズタマショウジョウバエにおける異所的な鐘状感覚子の形成に関わる遺伝子の探索　　　　　　　　　　　　　　　
平松楓佳, 越川滋行, 第68回日本動物学会北海道支部大会（北海道大学、口頭発表）, 16 Mar. 2024, [Last author]
Japanese, Summary national conference

wingless遺伝子の発現はミズタマショウジョウバエの翅模様形成に必要なのか？　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 第68回日本動物学会北海道支部大会（北海道大学、口頭発表）, 16 Mar. 2024, [Last author]
優秀発表賞, Japanese, Summary national conference

Identification of changes in regulatory sequence underlying the gain of polka-dotted pigmentation pattern in Drosophila guttifera　　　　　　　　　　　　　　　
Takumi Karasawa, Shigeyuki Koshikawa, TAGC24 (The Allied Genetics Conference 2024), Mar. 2024, [Last author]
Online poster, English, Summary international conference

ショウジョウバエの水玉模様をGal4/UASで改変する試み　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 日本進化学会第25回沖縄大会（ポスター発表）P-092Y, 02 Sep. 2023, [Last author]
Japanese, Summary national conference

Co-option of the ancestral cis-regulatory sequences underlying the gain of new wing pigmentation pattern in Drosophila guttifera　　　　　　　　　　　　　　　
Takumi Karasawa, Namiho Saito, Shigeyuki Koshikawa, 日本進化学会第25回沖縄大会（英語口頭発表）O1D-07Y, 01 Sep. 2023, [Last author]
English, Summary national conference

ショウジョウバエの水玉模様の獲得におけるcis制御の進化　　　　　　　　　　　　　　　
柄澤匠, 越川滋行, 第1回北海道バイオ"Mix up" （北海道大学農学部、札幌）口頭発表, 09 Aug. 2023, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエの翅の種特異的な模様の獲得における制御配列の進化と既存配列の転用　　　　　　　　　　　　　　　
柄澤匠, 齊藤奈美歩, 越川滋行, 日本動物学会 北海道支部 第67回大会（旭川市公民館木楽輪）口頭発表, 18 Mar. 2023, [Last author]
最優秀発表賞, Japanese, Summary national conference

ミズタマショウジョウバエにおけるGal4/UASシステムの開発　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 日本動物学会 北海道支部 第67回大会（旭川市公民館木楽輪）口頭発表, 18 Mar. 2023, [Last author]
Japanese, Summary national conference

洞窟種を含むチビゴミムシ亜科3種の視覚関連遺伝子の比較　　　　　　　　　　　　　　　
丹伊田 拓磨, 越川 滋行, 第67回日本応用動物昆虫学会大会（摂南大学、大阪）ポスター発表PS0314-03, 14 Mar. 2023, [Last author]
Japanese, Summary national conference

Development of Gal4/UAS system for elucidating the mechanism of the wing color pattern formation of Drosophila guttifera　　　　　　　　　　　　　　　
Masato Koseki, Shigeyuki Koshikawa, 64th Annual Drosophila Research Conference, 01 Mar. 2023, [Last author]
English, Summary international conference

Pre-existing Mad binding site is required for novel expression pattern of wingless in Drosophila guttifera pupal wing　　　　　　　　　　　　　　　
Takumi Karasawa, Namiho Saito, Shigeyuki Koshikawa, 64th Annual Drosophila Research Conference, 01 Mar. 2023, [Last author]
English, Summary international conference

ミズタマショウジョウバエの翅の新しい模様形成に必要なcis制御配列の特定　　　　　　　　　　　　　　　
柄澤匠, 齊藤奈美歩, 越川滋行, 日本動物学会第93回早稲田大会（口頭発表）3E37-1045, 10 Sep. 2022, [Last author], [Domestic magazines]
Japanese, Summary national conference

地下浅層に生息するチビゴミムシのオプ シン遺伝子と走光性　　　　　　　　　　　　　　　
丹伊田拓磨, 越川滋行, 日本昆虫学会第82回大会（松本）ポスター発表PB02, 04 Sep. 2022, [Domestic magazines]
最優秀ポスター賞, Japanese, Summary national conference

cis制御領域におけるSmadの結合配列の獲得が新しい遺伝子発現のパターンを生み出した　　　　　　　　　　　　　　　
柄澤 匠, 齊藤奈美歩, 越川滋行, 日本進化学会年大会 第24回沼津大会 ポスター発表, P-51, 05 Aug. 2022, [Last author]
Japanese, Summary national conference

野生種ショウジョウバエにおけるゲノム編集と模様研究への適用　　　　　　　　　　　　　　　
越川滋行, 日本動物学会北海道支部主催2021年度公開シンポジウム「野生動物のゲノム研究最前線」, 17 Dec. 2021, [Invited], [Lead author, Corresponding author]
Japanese, Lecture materials

ミズタマショウジョウバエを用いた Wingless モルフォゲンによる模様形成機構の解析　　　　　　　　　　　　　　　
福冨雄一, 重信秀治, 越川滋行, 日本遺伝学会第93回大会 2D-05 オンライン口頭発表, 09 Sep. 2021, [Last author]
Summary national conference

Mechanism of formation and function of wing pigmentation pattern in Drosophila guttifera　　　　　　　　　　　　　　　
越川滋行, 古関将斗, 丹伊田拓磨, 福冨雄一, 日本遺伝学会第93回大会 WS4: 形と行動の多様性の遺伝学 ショウジョウバエ近縁種を用いた研究から オンライン口頭発表, 08 Sep. 2021, [Invited], [Lead author]
Japanese, Summary national conference

The polka-dotted pattern in a fruit fly is generated by the evolution of the toolkit gene expression in specific accessory cells of nerve tissues　　　　　　　　　　　　　　　
古関 将斗, 田中 暢明, 越川 滋行, 日本動物学会第92回オンライン米子大会 ポスター発表E-10, 02 Sep. 2021, [Last author]
Japanese, Summary national conference

Winglessモルフォゲンはどのようにミズタマショウジョウバエの模様を形成するのか？　　　　　　　　　　　　　　　
福冨雄一, 重信秀治, 越川滋行, 日本進化学会第23回東京大会 シンポジウム 9: 昆虫とクマムシのユニークネスを科学しよう! オンライン口頭発表, 19 Aug. 2021
Summary national conference

模様形成遺伝子winglessを発現する神経組織の構成細胞の特定　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 日本進化学会第23回オンライン大会 ポスター発表, 19 Aug. 2021, [Last author]
学生発表賞【学部・修士課程】最優秀賞, Summary national conference

Two types of cells composing a campaniform sensillum express the patterning gene wingless in pupal wings of Drosophila guttifera.　　　　　　　　　　　　　　　
Masato Koseki, Nobuaki Tanaka, Shigeyuki Koshikawa, 62nd Annual Drosophila Research Conference, online poster, 30 Mar. 2021, [Last author]
English, Summary international conference

ミズタマショウジョウバエの遺伝子変異系統を用いた模様の機能に関する研究　　　　　　　　　　　　　　　
丹伊田 拓磨, 越川 滋行, 第65回日本応用動物昆虫学会大会（島根大学、オンライン）ポスター（PS31）, 24 Mar. 2021, [Last author]
Japanese, Summary national conference

Which cell type of campaniform sensilla expresses the patterning gene wingless in pupal wings of Drosophila guttifera?　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 日本進化学会第22回オンライン大会, ポスター, PA-95, 08 Sep. 2020, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエ胸部における模様形成メカニズムの探索　　　　　　　　　　　　　　　
矢野根智広, 越川滋行, 日本動物学会第91回大会オンライン, ポスター, D-14, 05 Sep. 2020, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエの模様形成遺伝子winglessは、蛹期の翅において鐘状感覚子のどの細胞で発現しているのか？　　　　　　　　　　　　　　　
古関将斗, 越川滋行, 日本動物学会第91回大会オンライン, ポスター, E-01, 04 Sep. 2020, [Last author]
Japanese, Summary national conference

ミズタマショウジョウバエを用いた模様形成遺伝子ネットワークの探索　　　　　　　　　　　　　　　
福冨雄一, 重信秀治, 越川滋行, 日本動物学会第91回大会オンライン, ポスター, B-15, 04 Sep. 2020, [Last author]
Summary national conference

ミズタマショウジョウバエの水玉模様が配偶者選択により進化した可能性　　　　　　　　　　　　　　　
丹伊田拓磨, 越川滋行, 第64回日本応用動物昆虫学会大会（新型コロナウイルス感染症の感染拡大によりみなし開催）, ポスター, PS025, 16 Mar. 2020, [Last author]
Japanese, Summary national conference

Transcriptome analysis of Drosophila guttifera to elucidate the gene network related to color pattern formation　　　　　　　　　　　　　　　
Yuichi Fukutomi, Shuji Shigenobu, Shigeyuki Koshikawa, 5th Asia-Pacific Drosophila Research Conference, 09 Jan. 2020
English, Summary international conference

Thoughts on function of Drosophila guttifera wing color pattern　　　　　　　　　　　　　　　
Takuma Niida, Shigeyuki Koshikawa, 5th Asia-Pacific Drosophila Research Conference, Jan. 2020
English, Summary international conference

Pigmentation pattern formation in Drosophila guttifera　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Keiji Matsumoto, Wataru Yamamoto, Yuichi Fukutomi, 5th Asia-Pacific Drosophila Research Conference, Jan. 2020
English, Summary international conference

Mechanism of color pattern formation in Drosophila guttifera　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Yuichi Fukutomi, Keiji Matsumoto, 90th Annual Meeting of the Zoological Society of Japan, S-23, Genetic mutations, development and physiology, and fitness: Toward understanding evolution and biodiversity, Sep. 2019, [Invited]
Japanese, Summary national conference

Exploration of genes involved in color pattern formation ~ transcriptome analysis of Drosophila guttiera ~　　　　　　　　　　　　　　　
Yuichi Fukutomi, Shuji Shigenobu, Shigeyuki Koshikawa, 日本進化学会 第21回 北海道大会 シンポジウム S10 模様研究のあした ~ 形成機構や機能 ~, Aug. 2019
Japanese, Summary national conference

Transcriptome analysis for elucidating genes expressed in the color pattern of Drosophila guttifera　　　　　　　　　　　　　　　
Yuichi Fukutomi, Shuji Shigenobu, Shigeyuki Koshikawa, 日本発生生物学会 第52回大会 大阪, May 2019
Japanese, Summary national conference

Enhancer function and evolution of polka-dots in Drosophila guttifera　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Yuichi Fukutomi, 52nd Meeting of JAPANESE SOCIETY of DEVELOPMENTAL BIOLOGISTS, Symposium 4:Enhancer function explaining morphological diversity, May 2019, [Invited]
English, Summary national conference

Generation mechanism of a polka-dot pattern in Drosophila guttifera.　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Keiji Matsumoto, Yuichi Fukutomi, The 46th Naito Conference Short Talk, Oct. 2018, [Peer-reviewed]
English, Summary international conference

ミズタマショウジョウバエの模様はどのように作られるのか？　　　　　　　　　　　　　　　
越川滋行, 福冨雄一, 松本圭司, 日本動物学会第89回大会 S3 遺伝的変異―発生・生理―適応度を繋ぐ生物多様性研究, Sep. 2018, [Invited]
Japanese, Summary national conference

カイコyellow遺伝子の発現制御領域のエンハンサー解析とシスモジュール制御　　　　　　　　　　　　　　　
鈴木誉保, 越川滋行, 小林功, 内野恵郎, 瀬筒秀樹, 蚕糸・昆虫機能利用学術講演会（日本蚕糸学会第88回大会）, Mar. 2018
Japanese, Summary national conference

ミズタマショウジョウバエの翅の着色過程の解明と模様形成遺伝子同定法　　　　　　　　　　　　　　　
福冨雄一, 松本圭司, 船山典 子, 越川滋行, 日本進化学会第19回大会, Aug. 2017
Japanese, Summary national conference

ミズタマショウジョウバエの模様形成の仕組み　　　　　　　　　　　　　　　
越川滋行, 蚕糸・昆虫機能利用学術講演会（日本蚕糸学会第87回大会）, 21 Mar. 2017, [Invited]
Japanese, Summary national conference

Origins of evolutionary novelty: Developmental regulation and evolution of color pattern formation in a polka-dotted fruit fly, Drosophila guttifera.　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, New Frontiers of Genetics, 26 Dec. 2016, [Invited]
English, Summary national conference

Wing color pattern formation in a polka-dotted fruit fly Drosophila guttifera　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Yuichi Fukutomi, Keiji Matsumoto, The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan, 17 Nov. 2016
English, Summary national conference

Unique mechanism of pigmentation pattern formation in the polka-dotted fly, Drosophila guttifera　　　　　　　　　　　　　　　
Yuichi Fukutomi, Noriko Funayama, Shigeyuki Koshikawa, The Joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan, 17 Nov. 2016
English, Summary national conference

Toward understanding the mechanism of color pattern formation in Drosophila guttifera　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Yuichi Fukutomi, Keiji Matsumoto, The 88th Annual Meeting of the Genetics Society of Japan, Sep. 2016, [Invited]
Japanese, Summary national conference

Drosophila guttifera as a model system for elucidating color pattern formation　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Yuichi Fukutomi, Keiji Matsumoto, Integrative Approach to Understanding the Diversity of Butterfly Wing Patterns, 02 Aug. 2016
English, Summary national conference

Wing development and pigmentation process in pupae of polka-dotted fly Drosophila guttifera　　　　　　　　　　　　　　　
Yuichi Fukutomi, Noriko Funayama, Kiyokazu Agata, Shigeyuki Koshikawa, JSDB Special Symposium: Frontier of Developmental Biology (+ 49th Meeting), 02 Jun. 2016
English, Summary national conference

ミズタマショウジョウバエの蛹期の翅の発生様式と斑紋形成プロセス
福冨雄一, 阿形清和, 越川滋行, 日本応用動物昆虫学会大会講演要旨, 60th, 171, 10 Mar. 2016
Japanese

Generation and Evolution of Polka-Dots in Drosophila guttifera
Shigeyuki Koshikawa, The 88th Annual Meeting of the Japanese Biochemical Society, 88th, 3W8-2 (WEB ONLY), Dec. 2015, [Invited]
Japanese, Summary national conference

[S02] 転写制御と遺伝的変異の発生と進化 －ゲノム情報、ゲノム編集、エンハンサー解析－
鈴木誉保, 越川滋行, 日本進化学会ニュース, 16, 3, 12, 13, Nov. 2015, [Invited]
Japanese, Meeting report

Genetic changes underlying color pattern evolution　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, 17th Annual Meeting of Society of Evolutionary Studies, Japan, S-02, Aug. 2015, [Invited]
Japanese, Summary national conference

Toward understanding color pattern formation and evolution in Drosophila　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Matt W. Giorgianni, Kathy Vaccaro, Victoria A. Kassner, John H. Yoder, Thomas Werner, Sean B. Carroll, 48th ANNUAL MEETING of the JAPANESE SOCIETY of DEVELOPMENTAL BIOLOGISTS, OP01-3, Jun. 2015
English, Summary national conference

ショウジョウバエの翅模様を制御する因子の発現パターンの進化
KOSHIKAWA SHIGEYUKI, 日本応用動物昆虫学会大会講演要旨, 59th, 114, Mar. 2015
Japanese, Summary national conference

Presoldier cuticle contributes to the soldier morphogenesis in termites　　　　　　　　　　　　　　　
Sugime Y, Ogawa K, Watanabe D, Shimoji S, Koshikawa S, Miura T, 17th Congress of the International Union for the Study of Social Insects (IUSSI), OR110, Jul. 2014
English, Summary international conference

Evolution of cis-regulatory elements for the wingless gene in Drosophila guttifera　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Matt W. Giorgianni, Kathy Vaccaro, Victoria A. Kassner, John H. Yoder, Thomas Werner, Sean B. Carroll, JDRC11, S9, Jun. 2014
English, Summary national conference

Dachshundによるシロアリ兵隊の大顎伸長制御
SUGIME YASUHIRO, KOSHIKAWA SHIGEYUKI, MIURA TORU, 日本応用動物昆虫学会大会講演要旨, 58th, 151, Mar. 2014
Japanese, Summary national conference

Gain of wingless expression domains by evolution of cis-regulatory elements　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Matt W. Giorgianni, Thomas Werner, Kathy Vaccaro, Victoria Kassner, John H Yoder, Sean B Carroll, 15th Annual Meeting of Society of Evolutionary Studies, Japan, Aug. 2013
Japanese, Summary national conference

Evolution of a novel wing pigmentation pattern in Drosophila : when engrailed crosses the line　　　　　　　　　　　　　　　
Héloïse D. Dufour, Cédric Finet, Shigeyuki Koshikawa, Jane E. Selegue, Sean B. Carroll, 54th Annual Drosophila Research Conference, Apr. 2013
English, Summary international conference

遺伝子発現の時空―ショウジョウバエの水玉模様を追って
KOSHIKAWA SHIGEYUKI, KOSHIKAWA SHIGEYUKI, 日本動物学会大会予稿集, 82nd, 78, Aug. 2011, [Invited]
Japanese, Summary national conference

Sociogenomics in Termites: Caste Development and Underlying Developmental and Physiological Systems　　　　　　　　　　　　　　　
Miura T, Cornette R, Ishikawa Y, Hattori A, Gotoh H, Hayashi Y, Koshikawa S, 20th CDB Meeting Kobe, Feb. 2011
English, Summary international conference

Evolution of novel and complex traits　　　　　　　　　　　　　　　
Shigeyuki Koshikawa, Thomas Werner, Thomas M Williams, Sean B Carroll, 11th Annual Meeting of Society of Evolutionary Studies, Japan, W2D, Sep. 2009, [Invited]
English, Summary national conference

幼若ホルモンがクワガタのアロメトリーを決定する　　　　　　　　　　　　　　　
後藤 寛貴, コルネット リシャー, 越川 滋行, 岡田 泰和, 三浦 徹, 日本進化学会第11回大会, Aug. 2009
Japanese, Summary national conference

Juvenile hormone determines the scaling relationship in stag beetle　　　　　　　　　　　　　　　
Gotoh H, Cornette R, Koshikawa S, Okada Y, Miura T, Evolution 2009, Jun. 2009
English, Summary national conference

クワガタの大顎サイズに環境が与える影響とその発生学的背景について　　　　　　　　　　　　　　　
後藤 寛貴, コルネット リシャー, 越川 滋行, 岡田 泰和, 三浦 徹, 日本昆虫学会第68回大会, Sep. 2008
Japanese, Summary national conference

クマムシの一種ヨコヅナクマムシの生活史
HORIKAWA HIROKI, KOSHIKAWA SHIGEYUKI, ABE WATARU, NAKAHARA YU, WATANABE TADAHIKO, AZUMA MASATAKA, MIURA TOORU, KUNIEDA TAKEKAZU, OKUDA TAKASHI, 日本応用動物昆虫学会大会講演要旨, 52nd, 152, Mar. 2008
Japanese, Summary national conference

クワガタにおける誇張された形態の発生学的研究　　　　　　　　　　　　　　　
Hiroki Gotoh, Shigeyuki Koshikawa, Richard Cornette, Yasukazu Okada, Toru Miura, 日本応用動物昆虫学会・日本昆虫学会共催 北海道支部大会, Feb. 2008
Japanese, Summary national conference

シロアリのカースト分化と社会行動の分子基盤
MIURA TOORU, ISHIKAWA YUKI, CORNETTE RICHARD, KOSHIKAWA SHIGEYUKI, 日本動物学会大会要旨集, 78th, 122, Aug. 2007
Japanese, Summary national conference

オオシロアリのカースト調節に関わる候補遺伝子の解析
KOSHIKAWA SHIGEYUKI, HATTORI AKIKO, MIURA TOORU, 日本動物学会大会要旨集, 78th, 33, Aug. 2007
Japanese, Summary national conference

シロアリのカースト分化機構はどこまでわかったか
MIURA TOORU, KOSHIKAWA SHIGEYUKI, CORNETTE RICHARD, FUJITA AI, ISHIKAWA YUKI, HOJO YU, MAEKAWA KIYOTO, MATSUMOTO TADAO, 日本生態学会大会講演要旨集, 53rd, 207, Mar. 2006
Japanese, Summary national conference

ヤマトシロアリの生殖虫分化と複眼の発達
MAEKAWA KIYOTO, MIZUNO SAYAKA, KOSHIKAWA SHIGEYUKI, MIURA TOORU, 日本生態学会大会講演要旨集, 53rd, 207, Mar. 2006
Japanese, Summary national conference

チョウの斑紋多様性と進化－統合的アプローチ－
関村利朗, 藤原晴彦, 大瀧丈二, 監修, 関村 利朗, 藤原 晴彦, 大瀧 丈二, 16章
海游舎, Nov. 2017, 4905930596, 408, Japanese, Scholarly book, [Contributor]

エコゲノミクス ―遺伝子からみた適応― (シリーズ 現代の生態学 7)
森長 真一, 工藤 洋, 日本生態学会, 第17章 エボデボと新しい総合 越川 滋行
共立出版, Dec. 2012, 4320057406, 320, 263-277, Japanese, Scholarly book, [Contributor]

シロアリの事典
吉村 剛, 板倉修司, 岩田隆太郎, 大村和香子, 杉尾幸司, 竹松葉子, 徳田 岳, 松浦健二, 三浦 徹, 吉村 剛, 板倉修司, 岩田隆太郎, 大村和香子, 杉尾幸司, 竹松葉子, 徳田 岳, 松浦健二, 三浦 徹, 玉本 奈々, 4章2部「カースト分化の際の形態形成機構」越川滋行
海青社, Dec. 2012, 4860992601, 472, [Contributor]

ショウジョウバエの翅模様の進化発生生物学　　　　　　　　　　　　　　　
越川滋行
生命科学教室セミナー 東京都立大学, 09 Aug. 2024, Japanese, Public discourse
[Invited]

Developmental regulation and evolution of color pattern in fruit flies　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
Joint Symposium of School of Science & WPI-ICReDD in Hokkaido University, 10 Dec. 2020, English, Invited oral presentation
[Invited]

ハエの翅の模様が作られる仕組み　　　　　　　　　　　　　　　
越川滋行
北海道大学環境科学院EESセミナー, 03 Dec. 2019, Japanese, Public discourse
[Domestic Conference]

生き物の形の進化と、その仕組み　　　　　　　　　　　　　　　
越川滋行
北海道大学地球環境科学研究院 公開講座〈生物の「変化」：理由、メカニズム、そして影響〉, 24 Sep. 2019, Japanese, Public discourse
[Invited], [Domestic Conference]

ミズタマショウジョウバエの模様形成の仕組み　　　　　　　　　　　　　　　
越川滋行
岡山大学昆虫学土曜セミナー 第367回, 20 Jul. 2019, Japanese, Invited oral presentation
[Invited]

ミズタマショウジョウバエの模様形成の仕組み　　　　　　　　　　　　　　　
越川滋行
第4回北海道大学部局横断シンポジウム, 25 Jan. 2019, Japanese, Invited oral presentation
（口頭発表賞2位）, [Domestic Conference]

ミズタマショウジョウバエDrosophila guttiferaの模様ができる仕組みを調べ、機能を考える　　　　　　　　　　　　　　　
越川滋行
第5回生態進化発生コロキウム, 28 Dec. 2017, Japanese, Oral presentation
[Domestic Conference]

ミズタマショウジョウバエの水玉模様には意味があるのか？　　　　　　　　　　　　　　　
越川滋行, 松本圭司, 福冨雄一
5分で自分をアピール！生態学ライトニングトーク＠東京 第64回日本生態学会大会 自由集会, 15 Mar. 2017, Japanese, Oral presentation
[Domestic Conference]

模様形成を理解するためのモデル系としてのミズタマショウジョウバエ　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
モデル動物研究会「ムシサカナの会＠金沢」, 07 Oct. 2016, English, Invited oral presentation
[Invited], [Domestic Conference]

How did the novel pigmentation pattern of Drosophila appear?　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
遺伝研研究集会 分子進化学の現状と今後の展望, 20 Aug. 2016, English, Invited oral presentation
[Invited], [Domestic Conference]

模様はどのようにしてつくられるのか：ミズタマショウジョウバエを例に　　　　　　　　　　　　　　　
越川滋行
京都大学総合博物館 企画展 虫を知りつくす 京都大学の挑戦 特別講演会シリーズ 昆虫アカデミア, 06 Aug. 2016, Japanese, Public discourse
[Invited], [Domestic Conference]

ミズタマショウジョウバエの模様形成の仕組み　　　　　　　　　　　　　　　
越川滋行
昆虫デザイン研究会 家畜化過程の理解とポスト家畜化, 07 Jul. 2016, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

ミズタマショウジョウバエの模様が形成される仕組みと機能を考える　　　　　　　　　　　　　　　
越川滋行
Evo-Devo青年の会, 25 Jun. 2016, Japanese, Poster presentation
[Domestic Conference]

模様ができる仕組みとその意味　　　　　　　　　　　　　　　
越川滋行
シンポジウム 自然を問う理系 人間を問う文系 研究の原点とは, 19 Apr. 2016, Japanese, Public discourse
[Invited], [Domestic Conference]

Gain of cis-regulatory activities and evolution of novel traits in Drosophila　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
Drosophila Evolutionary Genetics Report, South China Agricultral University, 24 Oct. 2015, English, Public discourse
[Invited], [International presentation]

ショウジョウバエの模様形成と、動物の新しい形態の進化　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
富山大学理学部生物学科セミナー 兼第25回北陸社会性昆虫勉強会, 10 Mar. 2015, Japanese, Public discourse
[Invited], [Domestic Conference]

研究者として生きる-新しい価値を生み出す苦しみと喜び　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
佐高OB夢授業 千葉県立佐原高等学校, 13 Dec. 2014, Japanese, Public discourse
[Invited], [Domestic Conference]

ミズタマショウジョウバエの模様の進化　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
第262回 生態学研究センターセミナー, 12 Dec. 2014, Japanese, Public discourse
[Invited], [Domestic Conference]

生物学—動物の模様は何のためにあるのか？　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
京都大学ジュニアキャンパス, 20 Sep. 2014, Japanese, Public discourse
[Domestic Conference]

蝶の模様はどのようにつくられるのか？　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
京都大学サマースクール, 19 Aug. 2014, Japanese, Public discourse
[Domestic Conference]

ミズタマショウジョウバエの模様形成 ー新しい発現パターンはどのように獲得されるのか　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
名古屋大学IGERセミナー, 26 Jun. 2014, Japanese, Public discourse
[Invited], [Domestic Conference]

進化発生学の未来　　　　　　　　　　　　　　　
Shigeyuki Koshikawa
TKB_EvoDevo, 31 Aug. 2013, Japanese, Public discourse
[Invited], [Domestic Conference]

多様性生物学概論 (オムニバス)　　　　　　　　　　　　　　　
北海道大学大学院理学院
2017 - Present

生態学基礎論 (オムニバス)　　　　　　　　　　　　　　　
北海道大学大学院環境科学院
2017 - Present

科学・技術の世界 現代生物学への誘いII冬 (オムニバス)　　　　　　　　　　　　　　　
北海道大学全学教育科目
2017 - Present

環境生物学II　　　　　　　　　　　　　　　
北海道大学理学部
2017 - Present

生態遺伝学特論I　　　　　　　　　　　　　　　
北海道大学大学院環境科学院
2017 - Present

生物学II　　　　　　　　　　　　　　　
北海道大学全学共通科目
2017 - Present

進化発生生物学特別講義　　　　　　　　　　　　　　　
東京都立大学
Aug. 2024 - Aug. 2024

多様性生物学III　　　　　　　　　　　　　　　
北海道大学理学部
2017 - 2023

多様性生物学研究法 (オムニバス)　　　　　　　　　　　　　　　
北海道大学大学院理学院
2022 - 2022

生物科学特論II　　　　　　　　　　　　　　　
東京大学理学部
Sep. 2021 - Sep. 2021

新国際基盤生命学特論２　　　　　　　　　　　　　　　
東京大学大学院理学系研究科
Sep. 2021 - Sep. 2021, Postgraduate courses

自然科学実験 (分担)　　　　　　　　　　　　　　　
北海道大学全学教育科目
2017 - 2021

先端研究（オムニバス）　　　　　　　　　　　　　　　
慶應義塾大学大学院 政策・メディア研究科
Oct. 2020 - Oct. 2020, Postgraduate courses, Japan

教科教育法 (理科II) (分担)　　　　　　　　　　　　　　　
北海道大学教職専門科目
2019 - 2019

生物学特別講義I (集中講義)　　　　　　　　　　　　　　　
茨城大学大学院理工学研究科
Sep. 2018 - Sep. 2018

Global Frontier in Life Science A（オムニバス）　　　　　　　　　　　　　　　
京都大学大学院生命科学研究科
2014 - 2017

ILASセミナー　　　　　　　　　　　　　　　
京都大学全学共通科目
2016 - 2016

生物学実習Ｄ　　　　　　　　　　　　　　　
京都大学理学部
2015 - 2016

生物学セミナ－Ａ　　　　　　　　　　　　　　　
京都大学理学部
2015 - 2016

Interdisciplinary Life Science Course　　　　　　　　　　　　　　　
京都大学大学院理学研究科
2014 - 2016

生物科学課題研究２２　　　　　　　　　　　　　　　
京都大学理学部
2015 - 2015

いのちを知る科学　　　　　　　　　　　　　　　
京都大学全学共通科目
2015 - 2015

シリーズ白眉対談11 「生物学の様々な視点」
『白眉センターだより』第 12 号, 加賀谷勝史, 山道真人, 越川滋行, 原村隆司, Dec. 2016, [Others]

シリーズ白眉対談9 「夢」
『白眉センターだより』第 10 号, 石本健太, 上峯篤史, 榎戸輝揚, 林眞理, 山吉麻子, 越川滋行, Oct. 2015, [Others]

シリーズ白眉対談8 「アカデミズムと社会」
『白眉センターだより』第 9 号, 藤井崇, 江間有沙, 細将貴, 花田政範, Mar. 2015, 企画・取材・編集を行いました。, [Others]

環形動物シリスにおける無性生殖様式「ストロナイゼーション」の分子発生基盤の解明
科学研究費補助金 基盤研究(A)
Apr. 2018 - Mar. 2023
三浦徹
文部科学省, Competitive research funding

ゲノム編集で明らかにするショウジョウバエの模様形成機構
科学研究費補助金 基盤研究(B)
Apr. 2018 - Mar. 2023
越川滋行
文部科学省, Principal investigator, Competitive research funding

模様の機能を考える
科学研究費補助金 挑戦的研究(萌芽)
Jun. 2017 - Mar. 2020
越川滋行
文部科学省, Principal investigator, Competitive research funding

新奇性はどうやって生まれるのか？ゲノム編集によるアプローチ
科学研究費補助金 若手研究(B)
Apr. 2015 - Mar. 2017
越川 滋行
文部科学省, 若手研究(B), 京都大学, Principal investigator, Competitive research funding, 15K18586

細胞単離技術を用いて模様形成機構を解明する　　　　　　　　　　　　　　　
研究奨励金
Jan. 2015 - Mar. 2016
越川滋行
公益財団法人上原記念生命科学財団, Principal investigator, Competitive research funding

Mar. 2024 - Present
北海道大学出版会 企画委員

Apr. 2022 - Present
日本動物学会 広報委員

Sep. 2021 - Present
日本ショウジョウバエ研究会 世話役

Dec. 2020 - Present
日本進化学会 編集委員

Aug. 2024 - Jul. 2026
日本進化学会 理事（編集幹事）

Jul. 2024 - Mar. 2025
日本生態学会 第72回大会（札幌） 実行委員会

Sep. 2020 - Oct. 2024
日本動物学会 北海道支部 札樽地区委員

Jul. 2022 - Jun. 2024
日本進化学会 代議員

Apr. 2017 - Mar. 2023
文部科学省 科学技術・学術政策研究所 科学技術予測センター 専門調査員

2022
日本遺伝学会第94回大会（札幌）
実行委員

Jul. 2019 - Jun. 2021
日本動物学会 北海道支部 会計幹事

Aug. 2019 - Aug. 2019
日本進化学会第21回大会（札幌）
実行委員

Aug. 2018 - Aug. 2018
日本動物学会第89回大会（札幌）
実行委員会

Aug. 2017 - Aug. 2017
日本進化学会第19回大会（京都）
大会実行委員

Dec. 2013 - Oct. 2016
Evo-Devo青年の会 幹事