Apr. 2024, Japan Oil Chemists’ Society, JOCS Award　　　　　　　　　　　　　　　
水産脂溶性成分の有効利用を目指した分子栄養学的研究
細川雅史

2014, アスタキサンチン研究会, 9th Astaxanthin Award　　　　　　　　　　　　　　　
HOSOKAWA Masashi

2012, 日本油化学会, WCOS2012 Outstanding Poster Presentation Award　　　　　　　　　　　　　　　
細川 雅史

2007, 平成18年度日本油化学会進歩賞　　　　　　　　　　　　　　　
Japan

2003, The American Oil Chemists' Society, Biotechnology Division, Outstanding Paper Presentation.　　　　　　　　　　　　　　　

2003, The American Oil Chemists' Society, Health & Nutrition Devision, Best Overall Poster Award.　　　　　　　　　　　　　　　

2002, 平成14年度 日本油化学会オレオサイエンス賞　　　　　　　　　　　　　　　
Japan

2001, 平成13年度 日本水産学会奨励賞　　　　　　　　　　　　　　　
Japan

Identification of flexixanthin and its derivatives in Algoriphagus bacteria and evaluation of their antioxidant and anti-inflammatory effects
Naoki Takatani, Yuki Sato-Takabe, Misaki Aoike, Rika Sekine, Takashi Maoka, Tomoo Sawabe, Fumiaki Beppu, Kazutoshi Shindo, Masashi Hosokawa
Food Bioscience, 65, Mar. 2025
Scientific journal

Identification of a novel monocyclic carotenoid and prediction of its biosynthetic genes in Algoriphagus sp. oki45.
Naoki Takatani, Takashi Maoka, Tomoo Sawabe, Fumiaki Beppu, Masashi Hosokawa
Applied microbiology and biotechnology, 108, 1, 102, 102, Dec. 2024, [International Magazine]
English, Scientific journal, Bacteria belonging to the genus Algoriphagus have been isolated from various sources, such as Antarctic sea ice, seawater, and sediment, and some strains are known to produce orange to red pigments. However, the pigment composition and biosynthetic genes have not been fully elucidated. A new red-pigmented Algoriphagus sp. strain, oki45, was isolated from the surface of seaweed collected from Senaga-Jima Island, Okinawa, Japan. Genome comparison revealed oki45's average nucleotide identity of less than 95% to its closely related species, Algoriphagus confluentis NBRC 111222 T and Algoriphagus taiwanensis JCM 19755 T. Comprehensive chemical analyses of oki45's pigments, including 1H and 13C nuclear magnetic resonance and circular dichroism spectroscopy, revealed that the pigments were mixtures of monocyclic carotenoids, (3S)-flexixanthin ((3S)-3,1'-dihydroxy-3',4'-didehydro-1',2'-dihydro-β,ψ-caroten-4-one) and (2R,3S)-2-hydroxyflexixanthin ((2R,3S)-2,3,1'-trihydroxy-3',4'-didehydro-1',2'-dihydro-β,ψ-caroten-4-one); in particular, the latter compound was new and not previously reported. Both monocyclic carotenoids were also found in A. confluentis NBRC 111222 T and A. taiwanensis JCM 19755 T. Further genome comparisons of carotenoid biosynthetic genes revealed the presence of eight genes (crtE, crtB, crtI, cruF, crtD, crtYcd, crtW, and crtZ) for flexixanthin biosynthesis. In addition, a crtG homolog gene encoding 2,2'-β-hydroxylase was found in the genome of the strains oki45, A. confluentis NBRC 111222 T, and A. taiwanensis JCM 19755 T, suggesting that the gene is involved in 2-hydroxyflexixanthin synthesis via 2-hydroxylation of flexixanthin. These findings expand our knowledge of monocyclic carotenoid biosynthesis in Algoriphagus bacteria. KEY POINTS: • Algoriphagus sp. strain oki45 was isolated from seaweed collected in Okinawa, Japan. • A novel monocyclic carotenoid 2-hydroxyflexixanthin was identified from strain oki45. • Nine genes for 2-hydroxyflexixanthin biosynthesis were found in strain oki45 genome.

Identification of the Active EPA/AA-Binding Ether-Type Phosphatidylcholine Derived from the Starfish Patiria pectinifera for C2C12 Myotube Growth
Aoi Fukushima, Kyosuke Imamura, Naoki Takatani, Masashi Hosokawa, Fumiaki Beppu
ACS Omega, 12 Nov. 2024
Scientific journal

Dietary 7,10-dihydroxy-8(E)-octadecenoic acid reduces fat accumulation and prevents hyperglycemia and hyperlipidemia in diabetic/obese KK-Ay mice
Fumiaki Beppu, Shota Fuji, Hak-Ryul Kim, Ching T. Hou, Kazuo Miyashita, Masashi Hosokawa
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 61, Oct. 2024
English, Scientific journal

Apo-12'-capsorubinal exhibits anti-inflammatory effects and activates nuclear factor erythroid 2-related factor 2 in RAW264.7 macrophages.
Naoki Takatani, Hiroki Miyafusa, Yumiko Yamano, Fumiaki Beppu, Masashi Hosokawa
Archives of biochemistry and biophysics, 760, 110125, 110125, 16 Aug. 2024, [International Magazine]
English, Scientific journal, Apocarotenoids have short carbon chain structures cleaved at a polyene-conjugated double bond. They can be biosynthesized in plants and microorganisms. Animals ingest carotenoids through food and then metabolize them into apocarotenoids. Although several apocarotenoids have been identified in the body, their precise health functions are still poorly understood. This study investigated the anti-inflammatory activities of apo-12'-capsorubinal in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. It was confirmed that apo-12'-capsorubinal was not cytotoxic to the macrophages at the concentrations tested. Apo-12'-capsorubinal treatment led to a marked downregulation of interleukin (IL)-6 protein and Il6 mRNA levels. This apocarotenoid exhibited more potent inhibitory effects than its parent carotenoids, capsanthin and capsorubin. Furthermore, apo-12'-capsorubinal, but not its parent carotenoids, promoted the nuclear accumulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated the expression of Nrf2-target genes, such as heme oxygenase 1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO-1), in a dose-dependent manner. Furthermore, a comparison using apo-12'-zeaxanthinal and 7,8-dihydro-8-oxo-apo-12'-zeaxanthinal revealed that the α, β-unsaturated carbonyl group on the polyene linear chain mediated the enhanced nuclear Nrf2 translocation, HO-1 expression, and inhibition of IL-6 production. In contrast, apo-12'-mytiloxanthinal, which harbored a hydroxyl group at C-8 of apo-12'-capsorubinal, did not exhibit any of these activities. These results indicated that the β carbon of the α, β-unsaturated carbonyl group in the linear part of the polyene chain is crucial to the Nrf2-activating and anti-inflammatory effects of apo-12'-capsorubinal. This study will advance our knowledge of the physiological significance of xanthophyll-derived apocarotenoids and their potential use as nutraceuticals and pharmaceuticals.

Identification and tissue distribution of fucoxanthinol and amarouciaxanthin A fatty acid esters in fucoxanthin-fed mice
Naoki Takatani, Kana Sakimura, Kodai Nagata, Fumiaki Beppu, Yumiko Yamano, Takashi Maoka, Masashi Hosokawa
Food Chemistry, 410, 01 Jun. 2023
Scientific journal

Dietary Combination of Fish Oil and Soy β-Conglycinin Inhibits Fat Accumulation and Reduces Blood Glucose Levels by Altering Gut Microbiome Composition in Diabetic/Obese KK-<i>A</i> ^<i>y</i> Mice
Satoshi Wanezaki, Takumi Taniwaki, Junki Miyamoto, Masashi Hosokawa
Journal of Oleo Science, 72, 3, 303, 312, Japan Oil Chemists' Society, 2023
Scientific journal

Identification and tissue distribution of fucoxanthinol and amarouciaxanthin A fatty acid esters in fucoxanthin-fed mice.
Naoki Takatani, Kana Sakimura, Kodai Nagata, Fumiaki Beppu, Yumiko Yamano, Takashi Maoka, Masashi Hosokawa
Food chemistry, 410, 135318, 135318, 28 Dec. 2022, [International Magazine]
English, Scientific journal, Administered carotenoid fatty acid esters are thought to be hydrolyzed to their free forms and absorbed into the body, and information on the tissue distribution of carotenoid fatty acid esters has been limited. Fucoxanthin, a marine carotenoid, exhibits various health benefits, including anti-diabetic and anti-obesity effects. However, fucoxanthin metabolism in mammals remains unclear. Herein, we investigated the fatty acid esters of fucoxanthin metabolites, fucoxanthinol and amarouciaxanthin A, in the tissues of male C57BL/6J mice fed a fucoxanthin-containing diet for one week. Fucoxanthinol and amarouciaxanthin A-3-esters accumulated abundantly in the liver and epididymal white adipose tissue, respectively. These esters were less detectable in the serum and other tissues. Therefore, it is suggested that fucoxanthinol and amarouciaxanthin A are partially acylated in the liver and epididymal white adipose tissue after being transported through the body as their free forms. This study presents a novel carotenoid metabolic pathway in mammals.

Monocaprin Enhances Bioavailability of Fucoxanthin in Diabetic/Obese KK-Ay Mice
Kodai Nagata, Naoki Takatani, Fumiaki Beppu, Aya Abe, Etsuko Tominaga, Tomohisa Fukuhara, Makoto Ozeki, Masashi Hosokawa
Marine Drugs, 20, 7, 446, 446, MDPI AG, 07 Jul. 2022, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal, Fucoxanthin is a marine carotenoid found in brown seaweeds and several microalgae. It has been reported that fucoxanthin has health benefits such as anti-obesity and anti-diabetic effects. To facilitate fucoxanthin applications in the food industry, it is important to improve its low bioavailability. We attempted the combined feeding of fucoxanthin-containing seaweed oil (SO) and monocaprin in a powder diet and analyzed the fucoxanthin metabolite contents in the liver, small intestine and serum of diabetic/obese KK-Ay mice. After 4 weeks of feeding with the experimental diets, the serum fucoxanthinol concentrations of the mice fed 0.2% SO and 0.5% monocaprin were higher than those of the 0.2% SO-fed mice. Furthermore, fucoxanthinol accumulation in the liver and small intestine tended to increase in a combination diet of 0.2% SO and 0.125–0.5% monocaprin compared with a diet of 0.2% SO alone, although amarouciaxanthin A accumulation was not different among the 0.2% SO-fed groups. These results suggest that a combination of monocaprin with fucoxanthin-containing SO is an effective treatment for improving the bioavailability of fucoxanthin.

Preparation of Apoastaxanthinals and Evaluation of Their Anti-inflammatory Action against Lipopolysaccharide-Stimulated Macrophages and Adipocytes
Naoki Takatani, Fumiaki Beppu, Yumiko Yamano, Takashi Maoka, Kazuo Miyashita, Masashi Hosokawa
ACS OMEGA, Jun. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Reduction of serum lipid levels in diabetic obese KK-A ^y mice using monoalkyldiacylglycerol-rich oil derived from Berryteuthis magister
Masaki Kakimoto, Naoki Takatani, Masashi Hosokawa, Fumiaki Beppu
Fisheries Science, 2022
Scientific journal

n-3 Polyunsaturated fatty acid-enriched phosphatidylglycerol suppresses inflammation in RAW264.7 cells through Nrf2 activation via alteration of fatty acids in cellular phospholipids
Liping Chen, Fumiaki Beppu, Naoki Takatani, Kazuo Miyashita, Masashi Hosokawa
FISHERIES SCIENCE, 87, 5, 727, 737, Sep. 2021, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Seco-type β-Apocarotenoid Generated by β-Carotene Oxidation Exerts Anti-inflammatory Effects against Activated Macrophages.
Naoki Takatani, Fumiaki Beppu, Yumiko Yamano, Takashi Maoka, Masashi Hosokawa
Journal of oleo science, 70, 4, 549, 558, 02 Apr. 2021, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
English, Scientific journal, β-Apocarotenoids are the cleavage products of β-carotene. They are found in plants, carotenoid-containing foods, and animal tissues. However, limited information is available regarding the health benefits of β-apocarotenoids. Here, we prepared seco-type β-apocarotenoids through the chemical oxidation of β-carotene and investigated their anti-inflammatory effects against activated macrophages. Oxidation of β-carotene with potassium permanganate produced seco-β-apo-8'-carotenal, in which one end-group formed an "open" β-ring and the other was cleaved at the C-7',8' position. In lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells, seco-β-apo-8'-carotenal inhibited the secretion and mRNA expression of inflammatory mediators such as nitric oxide, interleukin (IL)-6 and IL-1β, and monocyte chemoattractant protein-1. Furthermore, seco-β-apo-8'-carotenal suppressed phosphorylation of c-Jun N-terminal kinase and the inhibitor of nuclear factor (NF)-κB as well as the nuclear accumulation of NF-κB p65. Notably, since seco-β-apo-8'-carotenal exhibited remarkable anti-inflammatory activity compared with β-apo-8'-carotenal, its anti-inflammatory action could depend on the opened β-ring structure. These results suggest that seco-β-apo-8'-carotenal has high potential for the prevention of inflammation-related diseases.

Effects of Fucoxanthin on the Inhibition of Dexamethasone-Induced Skeletal Muscle Loss in Mice
Maki Yoshikawa, Masashi Hosokawa, Kazuo Miyashita, Hoyoku Nishino, Takeshi Hashimoto
NUTRIENTS, 13, 4, Apr. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal

Health-Promoting Functions of the Marine Carotenoid Fucoxanthin
Masashi Hosokawa
CAROTENOIDS: BIOSYNTHETIC AND BIOFUNCTIONAL APPROACHES, 1261, 273, 284, 2021, [Invited], [Corresponding author]
English, Scientific journal

Identification of Paracentrone in Fucoxanthin-Fed Mice and Anti-Inflammatory Effect against Lipopolysaccharide-Stimulated Macrophages and Adipocytes
Naoki Takatani, Daisuke Taya, Ami Katsuki, Fumiaki Beppu, Yumiko Yamano, Akimori Wada, Kazuo Miyashita, Masashi Hosokawa
MOLECULAR NUTRITION & FOOD RESEARCH, 65, 2, e2000405, Jan. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

Black chokeberry (Aronia melanocarpa) juice residue and its ethanol extract decrease serum lipid levels in high‑fat diet‑fed C57BL/6J mice
Nana Mikami, Yukie Hosotani, Tomoko Saso, Tomoki Ohta, Kazuo Miyashita, Masashi Hosokawa
International Journal of Functional Nutrition, 1, 2, Spandidos Publications, 30 Oct. 2020, [Peer-reviewed], [Last author]
Scientific journal

Ecogenomics of the Marine Benthic Filamentous Cyanobacterium Adonisia
Juline M. Walter, Felipe H. Coutinho, Luciana Leomil, Paulo I. Hargreaves, Mariana E. Campeao, Veronica V. Vieira, Beatriz S. Silva, Giovana O. Fistarol, Paulo S. Salomon, Tomoo Sawabe, Sayaka Mino, Masashi Hosokawa, Hideaki Miyashita, Fumito Maruyama, Marcel C. van Verk, Bas E. Dutilh, Cristiane C. Thompson, Fabiano L. Thompson
MICROBIAL ECOLOGY, 80, 2, 249, 265, Aug. 2020, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal

Bioactive significance of fucoxanthin and its effective extraction
Kazuo Miyashita, Fumiaki Beppu, Masashi Hosokawa, Xiaoyong Liu, Shuzhou Wang
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 26, Jul. 2020, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal

Preparation of Phosphatidyl-panthenol by phospholipase D-mediated transphosphatidylation and its anti-inflammatory activity on macrophage-like RAW264.7 cells
Yukihiro Yamamoto, Kounosuke Suzuri, Tasuku Kunii, Hideyuki Kurihara, Kazuo Miyashita, Masashi Hosokawa
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 26, 101629, 101629, Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Fucoxanthin inhibits hepatic oxidative stress, inflammation, and fibrosis in diet-induced nonalcoholic steatohepatitis model mice
Naoki Takatani, Yuka Kono, Fumiaki Beppu, Yuko Okamatsu-Ogura, Yumiko Yamano, Kazuo Miyashita, Masashi Hosokawa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 528, 2, 305, 310, Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Nutraceutical characteristics of the brown seaweed carotenoid fucoxanthin
Kazuo Miyashita, Fumiaki Beppu, Masashi Hosokawa, Xiaoyong Liu, Shuzhou Wang
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 686, 108364, 108364, Jun. 2020, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal

Fucoxanthinol attenuates oxidative stress-induced atrophy and loss in myotubes and reduces the triacylglycerol content in mature adipocytes
Maki Yoshikawa, Masashi Hosokawa, Kazuo Miyashita, Takashi Fujita, Hoyoku Nishino, Takeshi Hashimoto
MOLECULAR BIOLOGY REPORTS, 47, 4, 2703, 2711, Apr. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effective extraction of carotenoids from brown seaweeds and vegetable leaves with edible oils
Kazuyoshi Teramukai, Shusuke Kakui, Fumiaki Beppu, Masashi Hosokawa, Kazuo Miyashita
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, 60, Mar. 2020, [Peer-reviewed]
English, Scientific journal

Spirulina Lipids Alleviate Oxidative Stress and Inflammation in Mice Fed a High-Fat and High-Sucrose Diet
Yuhong Yang, Lei Du, Masashi Hosokawa, Kazuo Miyashita
MARINE DRUGS, 18, 3, Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Preparation of n-3 Polyunsaturated Phosphatidylglycerol from Salmon Roe Lipids by Phospholipase D and In Vitro Digestion
Liping Chen, Fumiaki Beppu, Kazuo Miyashita, Masashi Hosokawa
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 122, 2, Feb. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effect of Spirulina lipids on high-fat and high-sucrose diet induced obesity and hepatic lipid accumulation in C57BL/6J mice
Yuhong Yang, Lei Du, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF FUNCTIONAL FOODS, 65, Feb. 2020, [Peer-reviewed]
English, Scientific journal

海洋性カロテノイドの健康機能性に関する最近の動向
高谷 直己, 細川 雅史
日本食品安全協会会誌, 15, 78, 85, 2020
Scientific journal

Total Lipids Content, Lipid Class and Fatty Acid Composition of Ten Species of Microalgae
Yuhong Yang, Lei Du, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 69, 10, 1181, 1189, 2020, [Peer-reviewed]
English, Scientific journal

The Effect of n-3 PUFA Binding Phosphatidylglycerol on Metabolic Syndrome-Related Parameters and n-3 PUFA Accretion in Diabetic/Obese KK-A(y) Mice
Liping Chen, Naoki Takatani, Fumiaki Beppu, Kazuo Miyashita, Masashi Hosokawa
NUTRIENTS, 11, 12, Dec. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Variation in Lipid Components from 15 Species of Tropical and Temperate Seaweeds
Eko Susanto, A. Suhaeli Fahmi, Masashi Hosokawa, Kazuo Miyashita
MARINE DRUGS, 17, 11, Nov. 2019, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal

Phospholipase A 2 -Mediated preparation of phosphatidylcholine containing ricinoleic acid and its anti-inflammatory effect on murine macrophage-like RAW264.7 cells
Yukihiro Yamamoto, Kazuki Harada, Suzuna Kasuga, Masashi Hosokawa
Biocatalysis and Agricultural Biotechnology, 19, Elsevier Ltd, 01 May 2019
English, Scientific journal

Carotenoid Profiling of a Red Seaweed Pyropia yezoensis: Insights into Biosynthetic Pathways in the Order Bangiales
Jiro Koizumi, Naoki Takatani, Noritoki Kobayashi, Koji Mikami, Kazuo Miyashita, Yumiko Yamano, Akimori Wada, Takashi Maoka, Masashi Hosokawa
MARINE DRUGS, 16, 11, Nov. 2018, [Peer-reviewed]
English, Scientific journal

Consumption of the edible sea urchin Mesocentrotus nudus attenuates body weight gain and hepatic lipid accumulation in mice
Ryoko Yamamoto, Hisanori Minami, Hiromi Matsusaki, Mami Sakashita, Naoki Morita, Osamu Nishimiya, Naonobu Tsutsumi, Masashi Hosokawa, Yutaka Itabashi, Toshiro Matsui, Kazuhiro Urad
JOURNAL OF FUNCTIONAL FOODS, 47, 40, 47, Aug. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Detection and identification of furan fatty acids from fish lipids by high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry
Hajime Uchida, Yutaka Itabashi, Ryuichi Watanabe, Ryoji Matsushima, Hiroshi Oikawa, Toshiyuki Suzuki, Masashi Hosokawa, Naonobu Tsutsumi, Kazuhiro Ura, Donato Romanazzi, Matthew R. Miller
FOOD CHEMISTRY, 252, 84, 91, Jun. 2018, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal

Acrolein as a Major Volatile in the Early Stages of Fish Oil TAG Oxidation
Ako Shibata, Mariko Uemura, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 67, 5, 515, 524, May 2018, [Peer-reviewed]
English, Scientific journal

Comparison of oxidative stability of monogalactosyl diacylglycerol, digalactosyl diacylglycerol, and triacylglycerol containing polyunsaturated fatty acids.　　　　　　　　　　　　　　　
Hazahari, N.Y, B, Hosokawa, M, Miyashita, K
Food Nutr. Sci., 9, 221, 234, 2018, [Peer-reviewed], [International Magazine]

Health impact of marine carotenoids.　　　　　　　　　　　　　　　
Miyashita, K, Hosokawa, M
J. Food Bioact. 1:31-40 (2018), 1, 34, 40, 2018, [Peer-reviewed]
Scientific journal

高度不飽和脂肪酸に対するスフィンゴイド塩基とα-トコフェロールの相乗的抗酸化効果　　　　　　　　　　　　　　　
宮下和夫, 上村麻梨子, 細川雅史
オレオサイエンス, 18, 317, 324, 2018, [Peer-reviewed]
Scientific journal

Effective Prevention of Oxidative Deterioration of Fish Oil: Focus on Flavor Deterioration
Kazuo Miyashita, Mariko Uemura, Masashi Hosokawa
ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 9, 9, 209, 226, 2018, [Peer-reviewed]
English, In book

Spatial and seasonal variations in the biofunctional lipid substances (fucoxanthin and fucosterol) of the laboratory-grown edible Japanese seaweed (Sargassum horneri Turner) cultured in the open sea
Masaru Terasaki, Chikara Kawagoe, Atsushi Ito, Hiroko Kumon, Bhaskar Narayan, Masashi Hosokawa, Kazuo Miyashita
SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 24, 7, 1475, 1482, Nov. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Docosapentaenoic Acid (22:5n-3) Downregulates mRNA Expression of Pro-inflammatory Factors in LPS-activated Murine Macrophage Like RAW264.7 Cells
Yanzhu Tian, Ami Katsuki, Donato Romanazzi, Matthew R. Miller, Serean L. Adams, Kazuo Miyashita, Masashi Hosokawa
JOURNAL OF OLEO SCIENCE, 66, 10, 1149, 1156, Oct. 2017, [Peer-reviewed]
English, Scientific journal

Fatty Acid and Lipid Class Composition of the Microalga Phaeodactylum tricornutum
Yu-Hong Yang, Lei Du, Masashi Hosokawa, Kazuo Miyashita, Yume Kokubun, Hisayoshi Arai, Hiroyuki Taroda
JOURNAL OF OLEO SCIENCE, 66, 4, 363, 368, Apr. 2017, [Peer-reviewed]
English, Scientific journal

Reduction of HbA1c levels by fucoxanthin-enriched akamoku oil possibly involves the thrifty allele of uncoupling protein 1 (UCP1): a randomised controlled trial in normal-weight and obese Japanese adults
Nana Mikami, Masashi Hosokawa, Kazuo Miyashita, Hitoshi Sohma, Yoichi M. Ito, Yasuo Kokai
JOURNAL OF NUTRITIONAL SCIENCE, 6, e5, Feb. 2017, [Peer-reviewed]
English, Scientific journal

Squalene modulates fatty acid metabolism: Enhanced EPA/DHA in obese/diabetic mice (KK-A(y)) model
Sangeetha Ravi Kumar, Ippei Yamauchi, Bhaskar Narayan, Ami Katsuki, Masashi Hosokawa, Kazuo Miyashita
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 118, 12, 1935, 1941, Dec. 2016, [Peer-reviewed]
English, Scientific journal

Bioconversion of Docosapentaenoic Acid in Human Cell Lines, Caco-2, HepG2, and THP-1
Yanzhu Tian, Donato Romanazzi, Kazuo Miyashita, Masashi Hosokawa
JOURNAL OF OLEO SCIENCE, 65, 12, 1017, 1022, Dec. 2016, [Peer-reviewed]
English, Scientific journal

Inhibitory Effect of Dihydrosphingosine with alpha-Tocopherol on Volatile Formation during the Autoxidation of Polyunsaturated Triacylglycerols
Mariko Uemura, Ako Shibata, Masashi Hosokawa, Ai Iwashima-Suzuki, Makoto Shiota, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 65, 9, 713, 722, Sep. 2016, [Peer-reviewed]
English, Scientific journal

Combined effect of astaxanthin and squalene on oxidative stress in vivo
Sangeetha Ravi Kumar, Bhaskar Narayan, Yuki Sawada, Masashi Hosokawa, Kazuo Miyashita
MOLECULAR AND CELLULAR BIOCHEMISTRY, 417, 1-2, 57, 65, Jun. 2016, [Peer-reviewed]
English, Scientific journal

Does squalene alter the antioxidant potential of astaxanthin and fucoxanthinol? In vitro evidence in RAW 264.7 cells, a murine macrophage
Sangeetha Ravi Kumar, Bhaskar Narayan, Yuki Kizawa, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 53, 4, 2139, 2143, Apr. 2016, [Peer-reviewed]
English, Scientific journal

Lipids, Fatty Acids, and Fucoxanthin Content from Temperate and Tropical Brown Seaweeds
Eko Susanto, Akhmad Suhaeli Fahmi, Masayuki Abe, Masashi Hosokawa, Kazuo Miyashita
2ND INTERNATIONAL SYMPOSIUM ON AQUATIC PRODUCTS PROCESSING AND HEALTH, ISAPPROSH 2015, 7, 66, 75, 2016, [Peer-reviewed]
English, International conference proceedings

A protocol for human serum fucoxanthinol quantitation using LC-MS/MS system.　　　　　　　　　　　　　　　
Mikami, N, Hosokawa, M, Abe, M, Miyashita, K, Sohma, H, Kokai, Y
J. Nutri. Med. Diet. Care., 2, 019, 2016, [Peer-reviewed]

The dietary effect of milk sphingomyelin on the lipid metabolism of obese/diabetic KK-A(y) mice and wild-type C57BL/6J mice
Ippei Yamauchi, Mariko Uemura, Masashi Hosokawa, Ai Iwashima-Suzuki, Makoto Shiota, Kazuo Miyashita
FOOD & FUNCTION, 7, 9, 3854, 3867, 2016, [Peer-reviewed]
English, Scientific journal

Dietary ALA from Spinach Enhances Liver n-3 Fatty Acid Content to Greater Extent than Linseed Oil in Mice Fed Equivalent Amounts of ALA
Miho Kuroe, Hiroyuki Kamogawa, Masashi Hosokawa, Kazuo Miyashita
LIPIDS, 51, 1, 39, 48, Jan. 2016, [Peer-reviewed]
English, Scientific journal

Formation of Acrolein in the Autoxidation of Triacylglycerols with Different Fatty Acid Compositions
Ako Shibata, Mariko Uemura, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 92, 11-12, 1661, 1670, Dec. 2015, [Peer-reviewed]
English, Scientific journal

The Beneficial Health Effects of Fucoxanthin
Kazuo Miyashita, Masashi Hosokawa
Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods: Second Edition, 122, 134, Wiley Blackwell, 05 Oct. 2015
English, In book

Anticancer effects of fucoxanthin and fucoxanthinol on colorectal cancer cell lines and colorectal cancer tissues
Kazuto Takahashi, Masashi Hosokawa, Hiroyuki Kasajima, Kazuteru Hatanaka, Kazuhiro Kudo, Norihiko Shimoyama, Kazuo Miyashita
ONCOLOGY LETTERS, 10, 3, 1463, 1467, Sep. 2015, [Peer-reviewed]
English, Scientific journal

Fucoxanthinol, Metabolite of Fucoxanthin, Improves Obesity-Induced Inflammation in Adipocyte Cells
Hayato Maeda, Shogo Kanno, Mei Kodate, Masashi Hosokawa, Kazuo Miyashita
MARINE DRUGS, 13, 8, 4799, 4813, Aug. 2015, [Peer-reviewed]
English, Scientific journal

Structure of a novel monocyclic carotenoid, 3 ''-hydroxy-2 '-isopentenylsaproxanthin ((3R,2 ' S)-2 -(3-hydroxy-3 -methylbuty1)-3 ', 4 '-didehydro-1 ', 2 '-dihydro-beta, Psi-carotene-3, 1 '-diol), from a flavobacterium Gillisia limnaea strain DSM 15749
Naoki Takatani, Tomoo Sawabe, Takashi Maoka, Kazuo Miyashita, Masashi Hosokawa
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 4, 2, 174, 179, Apr. 2015, [Peer-reviewed]
English, Scientific journal

褐藻カロテノイド、フコキサンチン
宮下和夫, 細川雅史
FFIジャーナル, 220, 2, 101, 109, FFIジャーナル編集委員会, 2015
Japanese

Identification of a novel carotenoid, 2 '-isopentenylsaproxanthin, by Jejuia pallidilutea strain 11shimoA1 and its increased production under alkaline condition
N. Takatani, K. Nishida, T. Sawabe, T. Maoka, K. Miyashita, M. Hosokawa
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98, 15, 6633, 6640, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Source-sink manipulation of Camelina sativa L. related to grain yield under stressful environment of Hokkaido, Japan
Kounosuke Fujita, Shohei Fujita, Takayuki Fujita, Satoshi Konishi, Johann Vollmann, Pravat Kumar Mohapatra, Masashi Hosokawa, Masato Kuwahara, Hirofumi Saneoka, Toshiaki Tadano
SOIL SCIENCE AND PLANT NUTRITION, 60, 2, 156, 161, Apr. 2014, [Peer-reviewed]
English, Scientific journal

The extracts of Japanese willow tree species are effective forapoptotic desperation or differentiation of acute myeloid leukemia cells
Kounosuke Fujita, Yuji Nomura, Masahiko Sawajiri, Pravat K. Mohapatra, Hany A. El-Shemy, Nguyen T. Nguyen, Masashi Hosokawa, Kazuo Miyashita, Teruo Maeda, Hirofumi Saneoka, Shohei Fujita, Takayuki Fujita
PHARMACOGNOSY MAGAZINE, 10, 38, 125, 131, Apr. 2014, [Peer-reviewed]
English, Scientific journal

Regulation of Polyunsaturated Fatty Acid Biosynthesis by Seaweed Fucoxanthin and Its Metabolite in Cultured Hepatocytes
Tsunehiro Aki, Masaya Yamamoto, Toshiaki Takahashi, Kohki Tomita, Rieko Toyoura, Kazuhiro Iwashita, Seiji Kawamoto, Masashi Hosokawa, Kazuo Miyashita, Kazuhisa Ono
LIPIDS, 49, 2, 133, 141, Feb. 2014, [Peer-reviewed]
English, Scientific journal

Carotenoids as a Nutraceutical Therapy for Visceral Obesity
Kazuo Miyashita, Masashi Hosokawa
Nutrition in the Prevention and Treatment of Abdominal Obesity, 329, 340, Elsevier Inc., 2014, [Peer-reviewed]
English, In book

Studies on chemical composition of three species of Enteromorpha
K. Ganesan, K. Suresh Kumar, P. V. Subba Rao, Y. Tsukui, N. Bhaskar, M. Hosokawa, K. Miyashita
Biomedicine and Preventive Nutrition, 4, 3, 365, 369, Elsevier Masson SAS, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequences of marine flavobacterium Nonlabens strains NR17, NR24, NR27, NR32, NR33, and Ara13
Masato Nakanishi, Pedro Meirelles, Ryohei Suzuki, Naoki Takatani, Sayaka Mino, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Moriya Ohkuma, Masashi Hosokawa, Kazuo Miyashita, Fabiano L. Thompson, Ako Niwa, Toko Sawabe, Tomoo Sawabe
Genome Announcements, 2, 6, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequences of marine flavobacterium Algibacter lectus strains SS8 and NR4
Naoki Takatani, Masato Nakanishi, Pedro Meirelles, Sayaka Mino, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Moriya Ohkuma, Masashi Hosokawa, Kazuo Miyashita, Fabiano L. Thompson, Ako Niwa, Toko Sawabe, Tomoo Sawabe
Genome Announcements, 2, 6, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequence of marine flavobacterium Jejuia pallidilutea strain 11shimoA1 and pigmentation mutants
Naoki Takatani, Masato Nakanishi, Pedro Meirelles, Sayaka Mino, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Moriya Ohkuma, Masashi Hosokawa, Kazuo Miyashita, Fabiano L. Thompson, Ako Niwa, Toko Sawabe, Tomoo Sawabe
Genome Announcements, 2, 6, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Fucoxanthin: A Marine Carotenoid Exerting Anti-Cancer Effects by Affecting Multiple Mechanisms
Sangeetha Ravi Kumar, Masashi Hosokawa, Kazuo Miyashita
MARINE DRUGS, 11, 12, 5130, 5147, Dec. 2013, [Peer-reviewed]
English

Chemical and nutritional characteristics of brown seaweed lipids: A review
Kazuo Miyashita, Nana Mikami, Masashi Hosokawa
JOURNAL OF FUNCTIONAL FOODS, 5, 4, 1507, 1517, Oct. 2013, [Peer-reviewed]
English

Oxidative Stability of Lipids Rich in EPA and DHA Extracted from Fermented Scallop Ovary
Naohiro Hamaoka, Junki Shimajiri, Masayuki Abe, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF FOOD SCIENCE, 78, 9, C1348, C1353, Sep. 2013, [Peer-reviewed]
English, Scientific journal

Synergistic Antioxidant Activity of Milk Sphingomyeline and Its Sphingoid Base with alpha-Tocopherol on Fish Oil Triacylglycerol
Junki Shimajiri, Makoto Shiota, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 61, 33, 7969, 7975, Aug. 2013, [Peer-reviewed]
English, Scientific journal

Seasonal variations of total lipids, fatty acid composition, and fucoxanthin contents of Sargassum horneri (Turner) and Cystoseira hakodatensis (Yendo) from the northern seashore of Japan
Masatoshi Nomura, Hiroyuki Kamogawa, Eko Susanto, Chikara Kawagoe, Hajime Yasui, Naotsune Saga, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF APPLIED PHYCOLOGY, 25, 4, 1159, 1169, Aug. 2013, [Peer-reviewed]
English, Scientific journal

Biosynthetic Pathway and Health Benefits of Fucoxanthin, an Algae-Specific Xanthophyll in Brown Seaweeds
Koji Mikami, Masashi Hosokawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 14, 7, 13763, 13781, Jul. 2013, [Peer-reviewed]
English

Down-Regulation of Hepatic Stearoyl-CoA Desaturase-1 Expression by Fucoxanthin via Leptin Signaling in Diabetic/Obese KK-A (y) Mice
Fumiaki Beppu, Masashi Hosokawa, Mi-Jin Yim, Taro Shinoda, Kazuo Miyashita
LIPIDS, 48, 5, 449, 455, May 2013, [Peer-reviewed]
English, Scientific journal

Substrate and Droplet Size: Important Factors for Understanding Aqueous Lipid Oxidation
Kazuo Miyashita, Masashi Hosokawa
Lipid Oxidation: Challenges in Food Systems, 155, 176, Elsevier Inc., 02 Apr. 2013, [Peer-reviewed]
English, In book

Bovine lactoferrin reduces visceral fat and liver triglycerides in ICR mice
Satoru Morishita, Tomoji Ono, Chikako Fujisaki, Yasuharu Ishihara, Michiaki Murakoshi, Hisanori Kato, Masashi Hosokawa, Kazuo Miyashita, Keikichi Sugiyama, Hoyoku Nishino
JOURNAL OF OLEO SCIENCE, 62, 2, 97, 103, Feb. 2013, [Peer-reviewed]
English, Scientific journal

Marine nutraceuticals: Sources, recovery and effective utilization　　　　　　　　　　　　　　　
Nimish Mol Stephen, N. Bhaskar, M. Hosokawa, K. Miyashita
Advances in Food Science and Technology, 1, 17, Nova Science Publishers, Inc., Jan. 2013
English, In book

Chemopreventive Effects of Astaxanthin on Inflammatory Bowel Disease and Inflammation-Related Colon Carcinogenesis
Masashi Hosokawa, Yumiko Yasui
Carotenoids and Vitamin A in Translational Medicine, 289, 306, 01 Jan. 2013
In book

Fucoxanthin as potential nutraceutical for obesity therapy: A Review.　　　　　　　　　　　　　　　
Miyashita K, Maeda H, Nishikawa S, Tsukui T, Widjaja-Adhi MA-K, Hosokawa M
Carotenoid Sci., 17, 1, 7, 2013, [Peer-reviewed]
English, Scientific journal

Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-A(y) mice
Fumiaki Beppu, Masashi Hosokawa, Yoshimi Niwano, Kazuo Miyashita
LIPIDS IN HEALTH AND DISEASE, 11, 112, Sep. 2012, [Peer-reviewed]
English, Scientific journal

Oxidative Stability of Glyceroglycolipids Containing Polyunsaturated Fatty Acids
Tomoyo Yamaguchi, Rumiko Sugimura, Junki Shimajiri, Masatake Suda, Masayuki Abe, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 61, 9, 505, 513, Sep. 2012, [Peer-reviewed]
English, Scientific journal

Effect of Caffeine and Capsaicin on the Blood Glucose Levels of Obese/Diabetic KK-A(gamma) Mice
Tomoyuki Okumura, Takayuki Tsukui, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 61, 9, 515, 523, Sep. 2012, [Peer-reviewed]
English, Scientific journal

Stability of Fucoxanthin in Dried Undaria Pinnatifida (Wakame) and Baked Products (Scones) Containing Wakame Powder
Rumiko Sugimura, Masatake Suda, Ayumi Sho, Tomoya Takahashi, Tokutake Sashima, Masayuki Abe, Masashi Hosokawa, Kazuo Miyashita
FOOD SCIENCE AND TECHNOLOGY RESEARCH, 18, 5, 687, 693, Sep. 2012, [Peer-reviewed]
English, Scientific journal

Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-A(y) mice
Fumiaki Beppu, Masashi Hosokawa, Yoshimi Niwano, Kazuo Miyashita
LIPIDS IN HEALTH AND DISEASE, 11, 112, Sep. 2012, [Peer-reviewed]
English, Scientific journal

Dietary Combination of Fish Oil and Taurine Decreases Fat Accumulation and Ameliorates Blood Glucose Levels in Type 2 Diabetic/Obese KK-A(y) Mice
Nana Mikami, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF FOOD SCIENCE, 77, 6, H114, H120, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Fucoxanthin promotes translocation and induction of glucose transporter 4 in skeletal muscles of diabetic/obese KK-A(y) mice
Sho Nishikawa, Masashi Hosokawa, Kazuo Miyashita
PHYTOMEDICINE, 19, 5, 389, 394, Mar. 2012, [Peer-reviewed]
English, Scientific journal

Skin Photoprotection by Marine Carotenoids
Masashi Hosokawa
Marine Cosmeceuticals: Trends and Prospects, 2012
Scientific journal

Therapeutic Effect of Fucoxanthin on Metabolic Syndrome and Type 2 Diabetes
Kazuo Miyashita, Show Nishikawa, Masashi Hosokawa
Nutritional and Therapeutic Interventions for Diabetes and Metabolic Syndrome, 367, 379, Elsevier Inc., 2012
English, In book

Analyses on the functions and developments for the utilization technology of health functional components derived from fisheries products -Effective utilization and development for the next stage-
Hideki Ushio, Naohiro Gotoh, Reiko Nagasaka, Masashi Hosokawa
Nippon Suisan Gakkaishi (Japanese Edition), 78, 5, 1053, 1056, 2012
Japanese, Scientific journal

Carotenoid Profile of Edible Japanese Seaweeds: An Improved HPLC Method for Separation of Major Carotenoids
Masaru Terasaki, Bhaskar Narayan, Hiroyuki Kamogawa, Masatoshi Nomura, Nimish Mol Stephen, Chikara Kawagoe, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY, 21, 5, 468, 479, 2012, [Peer-reviewed]
English, Scientific journal

In vivo antioxidant activity of fucoxanthin on obese/diabetes KK-Ay mice.　　　　　　　　　　　　　　　
S. Iwasaki, M. A-K. Widjaja-Adhi, A. Koide, T. Kaga, S. Nakano, F. Beppu, M. Hosokawa, K. Miyashita
Food Nutr. Sci., 3, 1491, 1499, 2012, [Peer-reviewed]
English

Brown Seaweed Lipids as Potential Source of Omega-3 PUFA in Biological Systems
Kazuo Miyashita, Bhaskar Narayan, Takayuki Tsukui, Hiroyuki Kamogawa, Masayuki Abe, Masashi Hosokawa
Handbook of Marine Macroalgae: Biotechnology and Applied Phycology, 329, 339, John Wiley and Sons, 21 Nov. 2011
English, In book

Algal Carotenoids as Potent Antioxidants
Kazuo Miyashita, M. Airanthi K. Widjaja-Adhi, Masayuki Abe, Masashi Hosokawa
Handbook of Marine Macroalgae: Biotechnology and Applied Phycology, 403, 414, John Wiley and Sons, 21 Nov. 2011
English, In book

Cancer Chemopreventive Ability of Conjugated Linolenic Acids
Takuji Tanaka, Masashi Hosokawa, Yumiko Yasui, Rikako Ishigamori, Kazuo Miyashita
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 12, 11, 7495, 7509, Nov. 2011, [Peer-reviewed]
English

The effect of milk polar lipids separated from butter serum on the lipid levels in the liver and the plasma of obese-model mouse (KK-A(y))
Shiomi Watanabe, Tomoki Takahashi, Leo Tanaka, Yuko Haruta, Makoto Shiota, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF FUNCTIONAL FOODS, 3, 4, 313, 320, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Nitrocapsanthin and Nitrofucoxanthin, Respective Products of Capsanthin and Fucoxanthin Reaction with Peroxynitrite
Makoto Tsuboi, Hideo Etoh, Kyuki Kato, Hiroki Nakatugawa, Hideki Kato, Yasunori Maejima, Gaku Matsumoto, Hironobu Mori, Masashi Hosokawa, Kazuo Miyashita, Harukuni Tokuda, Nobukuni Suzuki, Takashi Maoka
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59, 19, 10572, 10578, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Dietary astaxanthin inhibits colitis and colitis-associated colon carcinogenesis in mice via modulation of the inflammatory cytokines
Yumiko Yasui, Masashi Hosokawa, Nana Mikami, Kazuo Miyashita, Takuji Tanaka
CHEMICO-BIOLOGICAL INTERACTIONS, 193, 1, 79, 87, Aug. 2011, [Peer-reviewed]
English, Scientific journal

The allenic carotenoid fucoxanthin, a novel marine nutraceutical from brown seaweeds
Kazuo Miyashita, Sho Nishikawa, Fumiaki Beppu, Takayuki Tsukui, Masayuki Abe, Andmasashi Hosokawa
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 91, 7, 1166, 1174, May 2011, [Peer-reviewed]
English

Effect of Brown Seaweed Lipids on Fatty Acid Composition and Lipid Hydroperoxide Levels of Mouse Liver
M. K. Widjaja-Adhi Airanthi, Naoya Sasaki, Sayaka Iwasaki, Nobuko Baba, Masayuki Abe, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59, 8, 4156, 4163, Apr. 2011, [Peer-reviewed]
English, Scientific journal

Suppressive Effects of Amarouciaxanthin A on 3T3-L1 Adipocyte Differentiation through Down-regulation of PPAR gamma and C/EBP alpha mRNA Expression
Mi-Jin Yim, Masashi Hosokawa, Yoshiyuki Mizushina, Hiromi Yoshida, Yasunori Saito, Kazuo Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59, 5, 1646, 1652, Mar. 2011, [Peer-reviewed]
English, Scientific journal

Synthesis of novel phospholipids that bind phenylalkanols and hydroquinone via phospholipase D-catalyzed transphosphatidylation
Yukihiro Yamamoto, Hideyuki Kurihara, Kazuo Miyashita, Masashi Hosokawa
NEW BIOTECHNOLOGY, 28, 1, 1, 6, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Comparative Antioxidant Activity of Edible Japanese Brown Seaweeds
M. K. Widjaja-Adhi Airanthi, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF FOOD SCIENCE, 76, 1, C104, C111, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Antiobesity Effects of Undaria Lipid Capsules Prepared with Scallop Phospholipids
Tomoko Okada, Yasuyuki Mizuno, Shinichi Sibayama, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF FOOD SCIENCE, 76, 1, H2, H6, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-A(y) mice
Masashi Hosokawa, Tatsuya Miyashita, Sho Nishikawa, Shingo Emi, TakaYuki Tsukui, Fumiaki Beppu, Tomoko Okada, Kazuo Miyashita
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 504, 1, 17, 25, Dec. 2010, [Peer-reviewed]
English, Scientific journal

Anti-obesity and anti-diabetic effects of allenic carotenoid, fucoxanthin
Kazuo Miyashita, Hayato Maeda, Tomoko Okada, Masayuki Abe, Masashi Hosokawa
AGRO FOOD INDUSTRY HI-TECH, 21, 6, 24, 27, Nov. 2010
English, Scientific journal

Value Addition to Seafood Processing Discards
Sachindra M. Nakkarike, Bhaskar Narayan, Masashi Hosokawa, Kazuo Miyashita
Handbook of Seafood Quality, Safety and Health Applications, 390, 401, Wiley-Blackwell, 02 Sep. 2010
English, In book

Purified Canola Lutein Selectively Inhibits Specific Isoforms of Mammalian DNA Polymerases and Reduces Inflammatory Response
Sho Horie, Chiaki Okuda, Takatoshi Yamashita, Kenichi Watanabe, Kouji Kuramochi, Masashi Hosokawa, Toshifumi Takeuchi, Makiko Kakuda, Kazuo Miyashita, Fumio Sugawara, Hiromi Yoshida, Yoshiyuki Mizushina
LIPIDS, 45, 8, 713, 721, Aug. 2010, [Peer-reviewed]
English, Scientific journal

Brown Sea Weed Lipids as Possible Source for Nutraceuticals and Functional Foods
M. Airanthi K. Widjaja-Adhi, Takayuki Tsukui, Masashi Hosokawa, Kazuo Miysahita
Biocatalysis and Biomolecular Engineering, 211, 223, John Wiley and Sons, 19 Jul. 2010, [Peer-reviewed]
English, In book

Suppressive effects of the marine carotenoids, fucoxanthin and fucoxanthinol on triglyceride absorption in lymph duct-cannulated rats
Megumi Matsumoto, Masashi Hosokawa, Noriko Matsukawa, Masahito Hagio, Aki Shinoki, Megumi Nishimukai, Kazuo Miyashita, Takaji Yajima, Hiroshi Hara
EUROPEAN JOURNAL OF NUTRITION, 49, 4, 243, 249, Jun. 2010, [Peer-reviewed]
English, Scientific journal

Effects of sea squirt (Halocynthia roretzi) lipids on white adipose tissue weight and blood glucose in diabetic/obese KK-A(y) mice
Nana Mikami, Masashi Hosokawa, Kazuo Miyashita
MOLECULAR MEDICINE REPORTS, 3, 3, 449, 453, May 2010, [Peer-reviewed]
English, Scientific journal

Radical scavenging and singlet oxygen quenching activity of extracts from Indian seaweeds
N. M. Sachindra, M. K. W. A. Airanthi, M. Hosokawa, K. Miyashita
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 47, 1, 94, 99, Jan. 2010, [Peer-reviewed]
English, Scientific journal

In vitro and in vivo evaluation of mutagenicity of fucoxanthin (FX) and its metabolite fucoxanthinol (FXOH)
Fumiaki Beppu, Yoshimi Niwano, Emiko Sato, Masahiro Kohno, Takayuki Tsukui, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF TOXICOLOGICAL SCIENCES, 34, 6, 693, 698, Dec. 2009, [Peer-reviewed]
English

Inhibition of proliferation of a hepatoma cell line by fucoxanthin in relation to cell cycle arrest and enhanced gap junctional intercellular communication
Cheng-Ling Liu, Yung-Sheng Huang, Masashi Hosokawa, Kazuo Miyashita, Miao-Lin Hu
CHEMICO-BIOLOGICAL INTERACTIONS, 182, 2-3, 165, 172, Dec. 2009, [Peer-reviewed]
English, Scientific journal

Effect of Marine Protein Supplementation on Lipid Profile of Growing Rats Compared to Soybean Protein and Casein
Bhasakar Narayan, Kohei Yamaguchi, Masashi Hosokawa, Kenji Fukunaga, Toshimasa Nishiyama, Kazuo Miyashita
FOOD SCIENCE AND BIOTECHNOLOGY, 18, 6, 1330, 1335, Dec. 2009, [Peer-reviewed]
English, Scientific journal

Anti-obesity and anti-diabetic effects of fucoxanthin on diet-induced obesity conditions in a murine model
Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Katsura Murakami-Funayama, Kazuo Miyashita
MOLECULAR MEDICINE REPORTS, 2, 6, 897, 902, Nov. 2009, [Peer-reviewed]
English, Scientific journal

Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice
Fumiaki Beppu, Yoshimi Niwano, Takayuki Tsukui, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF TOXICOLOGICAL SCIENCES, 34, 5, 501, 510, Oct. 2009, [Peer-reviewed]
English, Scientific journal

EVALUATION OF RECOVERABLE FUNCTIONAL LIPID COMPONENTS OF SEVERAL BROWN SEAWEEDS (PHAEOPHYTA) FROM JAPAN WITH SPECIAL REFERENCE TO FUCOXANTHIN AND FUCOSTEROL CONTENTS
Masaru Terasaki, Atsushi Hirose, Bhaskar Narayan, Yuta Baba, Chikara Kawagoe, Hajime Yasui, Naotsune Saga, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF PHYCOLOGY, 45, 4, 974, 980, Aug. 2009, [Peer-reviewed]
English, Scientific journal

Edible Japanese seaweed, wakame (Undaria pinnatifida) as an ingredient in pasta: Chemical, functional and structural evaluation
P. Prabhasankar, P. Ganesan, N. Bhaskar, A. Hirose, Nimishmol Stephen, Lalitha R. Gowda, M. Hosokawa, K. Miyashita
FOOD CHEMISTRY, 115, 2, 501, 508, Jul. 2009, [Peer-reviewed]
English, Scientific journal

Antiobesity Effect of Allenic Carotenoid, Fucoxanthin
Kazuo Miyashita, Masashi Hosokawa
Nutrigenomics and Proteomics in Health and Disease: Food Factors and Gene Interactions, 145, 160, Wiley-Blackwell, 12 Jun. 2009
English, In book

Effect of Droplet Size on the Oxidative Stability of Soybean Oil TAG and Fish Oil TAG in Oil-in-Water Emulsion
Goki Azuma, Naoko Kimura, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 58, 6, 329, 338, Jun. 2009
English, Scientific journal

Effect of Droplet Size on the Oxidative Stability of Soybean Oil TAG and Fish Oil TAG in Oil-in-Water Emulsion
Goki Azuma, Naoko Kimura, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 58, 6, 329, 338, Jun. 2009, [Peer-reviewed]
English, Scientific journal

Effect of alpha-tocopherol on the synthesis of phosphatidylglycerol catalyzed by phospholipase D in an aqueous system
Kounosuke Suzuri, Yukihiro Yamamoto, Masashi Hosokawa, Kazuo Miyashita
BIOTECHNOLOGY LETTERS, 31, 5, 719, 723, May 2009, [Peer-reviewed]
English, Scientific journal

Enhancement of hepatic docosahexaenoic acid and arachidonic acid contents in C57BL/6J mice by dietary fucoxanthin
Takayuki Tsukui, Nobuko Baba, Masashi Hosokawa, Tokutake Sashima, Kazuo Miyashita
FISHERIES SCIENCE, 75, 1, 261, 263, Feb. 2009, [Peer-reviewed]
English, Scientific journal

Bio-functions of Marine Carotenoids
Masashi Hosokawa, Tomoko Okada, Nana Mikami, Izumi Konishi, Kazuo Miyashita
FOOD SCIENCE AND BIOTECHNOLOGY, 18, 1, 1, 11, Feb. 2009, [Peer-reviewed]
English

Comparative evaluation of growth inhibitory effect of stereoisomers of fucoxanthin in human cancer cell lines
Y. Nakazawa, T. Sashima, M. Hosokawa, K. Miyashita
JOURNAL OF FUNCTIONAL FOODS, 1, 1, 88, 97, Jan. 2009, [Peer-reviewed]
English, Scientific journal

Eicosapentaenoic Acid Plays a Beneficial Role in Membrane Organization and Cell Division of a Cold-Adapted Bacterium, Shewanella livingstonensis Ac10
Jun Kawamoto, Tatsuo Kurihara, Kentaro Yamamoto, Makiko Nagayasu, Yasushi Tani, Hisaaki Mihara, Masashi Hosokawa, Takeshi Baba, Satoshi B. Sato, Nobuyoshi Esaki
JOURNAL OF BACTERIOLOGY, 191, 2, 632, 640, Jan. 2009, [Peer-reviewed]
English, Scientific journal

Anti-obesity and anti-diabetic effects of fucoxanthin on diet-induced obesity conditions in a murine model
Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Katsura Murakami-Funayama, Kazuo Miyashita
MOLECULAR MEDICINE REPORTS, 2, 6, 897, 902, 2009, [Peer-reviewed]
English, Scientific journal

Application of Phospholipases for Highly Functional Phospholipid Preparation
Masashi Hosokawa
Biocatalysis and Agricultural Biotechnology, 2009
Scientific journal

Anti-Obesity Effect of Allene Carotenoids, Fucoxanthin and Neoxanthin from Seaweeds and Vegetables
K. Miyashita, H. Maeda, T. Tsukui, T. Okada, M. Hosokawa
II INTERNATIONAL SYMPOSIUM ON HUMAN HEALTH EFFECTS OF FRUITS AND VEGETABLES: FAVHEALTH 2007, 841, 167, 171, 2009
English, International conference proceedings

ANTI-OBESITY AND ANTI-DIABETIC EFFECT OF ALLENIC CAROTENOID
Tomoko Okada, Hayato Maeda, Masashi Hosokawa, Kazuo Miyashita
ANNALS OF NUTRITION AND METABOLISM, 55, 92, 92, 2009, [Peer-reviewed]
English

Growth Inhibition and Induction of Apoptosis of Colon Cancer Cell Lines by Applying Marine Phospholipid
Zakir Hossain, Masashi Hosokawa, Koretaro Takahashi
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 61, 1, 123, 130, 2009, [Peer-reviewed]
English, Scientific journal

New C-37 Skeletal Carotenoid from the Clam, Paphia amabillis
Takashi Maoka, Naoshige Akimoto, Mi-Jin Yim, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 56, 24, 12069, 12072, Dec. 2008, [Peer-reviewed]
English, Scientific journal

Cold adaptation of eicosapentaenoic acid-less mutant of Shewanella livingstonensis Ac10 involving uptake and remodeling of synthetic phospholipids containing various polyunsaturated fatty acids
Sho Sato, Tatsuo Kurihara, Jun Kawamoto, Masashi Hosokawa, Satoshi B. Sato, Nobuyoshi Esaki
EXTREMOPHILES, 12, 6, 753, 761, Nov. 2008, [Peer-reviewed]
English, Scientific journal

Effects of Trans and Conjugated LC N-3 Polyunsaturated Fatty Acids on Lipid Composition and Abdominal Fat Weight in Rats
T. Okada, R. Noguchi, M. Hosokawa, K. Fukunaga, T. Nishiyama, N. Zaima, T. Hirata, K. Miyashita
JOURNAL OF FOOD SCIENCE, 73, 8, H201, H206, Oct. 2008, [Peer-reviewed]
English, Scientific journal

Antiobesity effect of fucoxanthin from edible seaweeds and its multibiological functions
Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo Miyashita
ACS Symposium Series, 993, 376, 388, American Chemical Society, 19 Sep. 2008
English, International conference proceedings

Synthesis of phosphatidylated-monoterpene alcohols catalyzed by phospholipase D and their antiproliferative effects on human cancer cells
Yukihiro Yamamoto, Masashi Hosokawa, Hideyuki Kurihara, Takashi Maoka, Kazuo Miyashita
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 18, 14, 4044, 4046, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Suppressive Effect of Neoxanthin on the Differentiation of 3T3-L1 Adipose Cells
Tomoko Okada, Miho Nakai, Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 57, 6, 345, 351, Jun. 2008, [Peer-reviewed]
English, Scientific journal

Preparation of phosphatidylated terpenes via phospholipase D-mediated transphosphatidylation
Yukihiro Yamamoto, Masashi Hosokawa, Hideyuki Kurihara, Kazuo Miyashita
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 85, 4, 313, 320, Apr. 2008, [Peer-reviewed]
English, Scientific journal

Multi-functional carotenoid in seaweeds: fucoxanthin
Kazuo Miyashita, Masashi Hosokawa
NIPPON SUISAN GAKKAISHI, 74, 2, 261, 262, Mar. 2008, [Peer-reviewed]
Japanese, Scientific journal

Suppressive Effects of Alloxanthin and Diatoxanthin from Halocynthia roretzi on LPS-induced Expression of Pro-inflammatory Genes in RAW264.7 Cells
Izumi Konishi, Masashi Hosokawa, Tokutake Sashima, Takashi Maoka, Kazuo Miyashita
JOURNAL OF OLEO SCIENCE, 57, 3, 181, 189, Mar. 2008, [Peer-reviewed]
English, Scientific journal

Enzymatic Production of Marine-Derived Protein Hydrolysates and Their Bioactive Peptides for Use in Foods and Nutraceuticals
Tomoko Okada, Masashi Hosokawa, Seigo Ono, Kazuo Miyashita
Biocatalysis and Bioenergy, 491, 519, John Wiley & Sons, Inc., 25 Feb. 2008, [Peer-reviewed]
English, In book

Composition, Functionality and Potential Applications of Seaweed Lipids
Bhaskar Narayan, Chandini S. Kumar, Tokutake Sashima, Hayato Maeda, Masashi Hosokawa, Kazuo Miyashita
Biocatalysis and Bioenergy, 463, 490, John Wiley & Sons, Inc., 25 Feb. 2008, [Peer-reviewed]
English, In book

Seaweed carotenoid, fucoxanthin, as a multi-functional nutrient
Hayato Maeda, Takayuki Tsukui, Tokutake Sashima, Masashi Hosokawa, Kazuo Miyashita
ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION, 17, 196, 199, 2008, [Peer-reviewed]
English, Scientific journal

Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites
Nakkarike M. Sachindra, Emiko Sato, Hayato Maeda, Masashi Hosokawa, Yoshimi Niwano, Masahiro Kohno, Kazuo Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 55, 21, 8516, 8522, Oct. 2007, [Peer-reviewed]
English, Scientific journal

Dietary combination of fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese/diabetic KK-A(y) mice
Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 55, 19, 7701, 7706, Sep. 2007, [Peer-reviewed]
English, Scientific journal

Occurrence of conjugated cyclopropanoid acid in purified fish oil
Naoto Horii, Shingo Arato, Bhaskar Narayan, Masashi Hosokawa, Tokutake Sashima, Kazuo Miyashita
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 84, 8, 749, 754, Aug. 2007, [Peer-reviewed]
English, Scientific journal

Oxidative stability of salmon and herring roe lipids and their dietary effect on plasma cholesterol levels of rats
Hiromi Moriya, Takashi Kuniminato, Masahi Hosokawa, Kenji Fukunaga, Toshimasa Nishiyama, Kazuo Miyashita
FISHERIES SCIENCE, 73, 3, 668, 674, Jun. 2007, [Peer-reviewed]
English, Scientific journal

Combination effect of herring roe lipids and proteins on plasma lipids and abdominal fat weight of mouse
H. Moriya, M. Hosokawa, K. Miyashita
JOURNAL OF FOOD SCIENCE, 72, 5, C231, C234, Jun. 2007, [Peer-reviewed]
English, Scientific journal

Fucoxanthin and fucoxanthinol enhance the amount of docosahexaenoic acid in the liver of KKAy obese/diabetic mice
Takayuki Tsukui, Kensuke Konno, Masashi Hosokawa, Hayato Maeda, Tokutake Sashima, Kazuo Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 55, 13, 5025, 5029, Jun. 2007, [Peer-reviewed]
English, Scientific journal

Therapy of myeloma in vivo using marine phospholipid in combination with agaricus blazei murill as immune respond activator.
Kentaro Murakawa, Kenji Fukunaga, Masatoshi Tanouchi, Masashi Hosokawa, Zakir Hossain, Koretaro Takahashi
J. Oleo Sci., 56, 4, 179, 188, Japan oil chemists' society, 2007, [Peer-reviewed]
English, Mushroom (Agaricus blazei Murill) extract has been reported to possess antitumor effects through immune activation. Here, we investigated the beneficial effects of combining A. blazei extract with marine phospholipids in comparison to A. blazei extract alone on myeloma sp2 tumor suppression when orally administrated. The experimental groups designed for sp2 tumor bearing BALB/c nu/nu mice were drinks of: (1)control; (2)1.0 mg/mL squid phospholipid liposome alone; (3)0.5 mg/mL A. blazei Murill water extract alone; (4)1.0 mg/mL squid phospholipid liposome with 0.5 mg/mL A. blazei Murill water extract in the form of those simple mixture; and (5)1.0 mg/mL squid phospholipid liposome with 0.5 mg/mL A. blazei Murill water extract partially encapsulated. Orally administrated volumes amounted to approximately 5 mL per day per mouse for all groups. A. blazei Murill water extract alone and squid phospholipid alone served groups show moderate tumor suppression with total administrations of approximately 105 mg/mouse for squid phospholipid through out the experimental term. When both A. blazei Murill water extract and squid phospholipid were administrated simultaneously in a simple mixture form, promotional effect on cancer tumor suppression was observed. And when A. blazei Murill water extract was partially encapsulated in the squid phospholipid liposomes with total administrations being 105 mg/mouse for squid phospholipid, effect on cancer tumor suppression was more pronounced. Though there was no statistically significant difference in tumor sizes between the simple mixture form administrated group i.e. group (4) and the partially encapsulated form administrated group i.e. group (5), the tumor vanished mouse was seen in the partially encapsulated form administrated group. Thus it was concluded that combinational administration of the A. blazei Murill water extract and the marine phospholipid may be useful in myeloma sp2 therapy.

Unsaturated phosphatidylethanolamine as effective synergist in combination with alpha-tocopherol.
Takenaka Atsushi, Hosokawa Masashi, Miyashita Kazuo
J. Oleo Sci., 56, 10, 511, 516, Japan Oil Chemists' Society, 2007, [Peer-reviewed]
English, The effects of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) on the oxidation of bonito oil triacylglycerol (TAG) were examined under the absence or presence of α-tocopherol. Synthesized PC or PE having saturated and unsaturated fatty acids in known position were used in this study. Unsaturated PC and PE promoted TAG oxidation under the absence of α-tocopherol. On the contrary, unsaturated PE showed synergistic antioxidant effect with α-tocopherol, while PC had little effect. The strongest synergistic effect was found by 1-palmitoyl (16:0, PA)-2-docosahexaenoyl (22:6n-3)-PE, followed by 1-PA-2-arachidonyl (20:4n-6)-PE, 1,2-dioleoyl (18:1n-9)-PE, and 1-PA-2-linoleoyl (18:2n-6)-PE, respectively.

Effect of medium-chain triacylglycerols on anti-obesity effect of fucoxanthin.
Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K
Journal of oleo science, 56, 12, 615, 621, 12, 2007, [Peer-reviewed]
English, Dietary effects of medium-chain triacylglycerols (MCT) and fucoxanthin (Fc) on abdominal fat weight were determined using KK-Ay obese mouse. Experimental diet contained MCT(0.9%), Fc (0.1%), or MCT (0.9%) +Fc (0.1%). The abdominal fat weight of mice fed with Fc was significantly lower than that of mice fed with MCT. Uncoupling protein 1 (UCP1), a key molecule for metabolic thermogenesis, was clearly expressed in the white adipose tissue (WAT) of mice fed Fc, but little expression in that of the mice fed MCT. The anti-obesity effect of Fc was increased by mixing Fc with MCT. This increase would be due to the increase in the absorption rate of Fc by MCT.

Occurrence of conjugated linolenic acids in purified soybean oil
Tomohisa Kinami, Naoto Horii, Bhaskar Narayan, Shingo Arato, Masashi Hosokawa, Kazuo Miyashita, Hironori Negishi, Junichi Ikuina, Ryuji Noda, Seiichi Shirasawa
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 84, 1, 23, 29, Jan. 2007, [Peer-reviewed]
English, Scientific journal

Unsaturated phosphatidylethanolamine as effective synergist in combination with alpha-tocopherol.
Takenaka A, Hosokawa M, Miyashita K
Journal of oleo science, 56, 10, 511, 516, 10, 2007, [Peer-reviewed]
English, The effects of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) on the oxidation of bonito oil triacylglycerol (TAG) were examined under the absence or presence of α-tocopherol. Synthesized PC or PE having saturated and unsaturated fatty acids in known position were used in this study. Unsaturated PC and PE promoted TAG oxidation under the absence of α-tocopherol. On the contrary, unsaturated PE showed synergistic antioxidant effect with α-tocopherol, while PC had little effect. The strongest synergistic effect was found by 1-palmitoyl (16:0, PA)-2-docosahexaenoyl (22:6n-3)-PE, followed by 1-PA-2-arachidonyl (20:4n-6)-PE, 1,2-dioleoyl (18:1n-9)-PE, and 1-PA-2-linoleoyl (18:2n-6)-PE, respectively.

9trans,11trans conjugated linoleic acid inhibits the development of azoxymethane-induced colonic aberrant crypt foci in rats
Yumiko Yasui, Rikako Suzuki, Hiroyuki Kohno, Shingo Miyamoto, Fumiaki Beppu, Masashi Hosokawa, Kazuo Miyashita, Takuji Tanaka
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 59, 1, 82, 91, 2007, [Peer-reviewed]
English, Scientific journal

Potent inhibitory effect of trans9, trans 11 isomer of conjugated linoleic acid on the growth of human colon cancer cells
Fumiaki Beppu, Masashi Hosokawa, Leo Tanaka, Hiroyuki Kohno, Takuji Tanaka, Kazuo Miyashita
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 17, 12, 830, 836, Dec. 2006, [Peer-reviewed]
English, Scientific journal

Catalpa seed oil rich in 9t,11t,13c-conjugated linolenic acid suppresses the development of colonic aberrant crypt foci induced by azoxymethane in rats
Rikako Suzuki, Yumiko Yasui, Hiroyuki Kohno, Shingo Miyamoto, Masashi Hosokawa, Kazuo Miyashita, Takuji Tanaka
ONCOLOGY REPORTS, 16, 5, 989, 996, Nov. 2006, [Peer-reviewed]
English, Scientific journal

Production of phosphatidylcholine containing conjugated linoleic acid mediated by phospholipase A(2)
Yukihiro Yamamoto, Masashi Hosokawa, Kazuo Miyashita
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 41, 3-4, 92, 96, Aug. 2006, [Peer-reviewed]
English, Scientific journal

Production of docosahexaenoic acid bounded phospholipids via phospholipase A(2) mediated bioconversion
Shinichi Awano, Koji Miyamoto, Masashi Hosokawa, Mitsumasa Mankura, Koretaro Takahashi
FISHERIES SCIENCE, 72, 4, 909, 911, Aug. 2006, [Peer-reviewed]
English, Scientific journal

Fucoxanthin and its metabolite, fucoxanthinol, suppress adipocyte differentiation in 3T3-L1 cells
Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Nobuyuki Takahashi, Teruo Kawada, Kazuo Miyashita
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 18, 1, 147, 152, Jul. 2006, [Peer-reviewed]
English, Scientific journal

Growth inhibition and apoptosis induction by all-trans-conjugated linolenic acids on human colon cancer cells
Yumiko Yasui, Masashi Hosokawa, Hiroyuki Kohno, Takuji Tanaka, Kazuo Miyashita
ANTICANCER RESEARCH, 26, 3A, 1855, 1860, May 2006, [Peer-reviewed]
English, Scientific journal

Growth inhibition and apoptosis induction by all-trans-conjugated linolenic acids on human colon cancer cells
Yumiko Yasui, Masashi Hosokawa, Hiroyuki Kohno, Takuji Tanaka, Kazuo Miyashita
Anticancer Research, 26, 3 A, 1855, 1860, 3A, May 2006, [Peer-reviewed]
English, Scientific journal

Inhibition properties of dipeptides from salmon muscle hydrolysate on angiotensin I-converting enzyme
S Ono, M Hosokawa, K Miyashita, K Takahashi
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 41, 4, 383, 386, Apr. 2006, [Peer-reviewed]
English, Scientific journal

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells
Zakir Hossain, Hideyuki Kurihara, Masashi Hosokawa, Koretaro Takahashi
MOLECULAR AND CELLULAR BIOCHEMISTRY, 285, 1-2, 155, 163, Apr. 2006, [Peer-reviewed]
English, Scientific journal

Effect of polyunsaturated fatty acid-enriched phosphatidylcholine and phosphatidylserine on butyrate-induced growth inhibition, differentiation and apoptosis in Caco-2 cells
Z Hossain, M Konishi, M Hosokawa, K Takahashi
CELL BIOCHEMISTRY AND FUNCTION, 24, 2, 159, 165, Mar. 2006, [Peer-reviewed]
English, Scientific journal

Production of value added phospholipids via phospholipase A2 mediated bioconversion from soy lysolecithin and docosahexaenoic acid rich free fatty acid mixture.
Fisheries Sci.,, 72, 909, 912, 2006, [Peer-reviewed]

Troglitazone and 9cis, 11trans, 13trans-conjugated linolenic acid: Comparison of their antiproliferative and apoptosis-inducing effects on different colon cancer cell lines
Y. Yasui, M. Hosokawa, H. Kohno, T. Tanaka, K. Miyashita
CHEMOTHERAPY, 52, 5, 220, 225, 2006, [Peer-reviewed]
English, Scientific journal

Halocynthiaxanthin and fucoxanthinol isolated from Halocynthia roretzi induce apoptosis in human leukemia, breast and colon cancer cells
Izumi Konishi, Masashi Hosokawa, Tokutake Sashima, Hidetaka Kobayashi, Kazuo Miyashita
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 142, 1-2, 53, 59, Jan. 2006, [Peer-reviewed]
English, Scientific journal

Occurrence of conjugated fatty acids in aquatic and terrestrial plants and their physiological effects　　　　　　　　　　　　　　　
Bhaskar Narayan, Masashi Hosokawa, Kazuo Miyashita
Nutraceutical and Specialty Lipids and their Co-Products, 201, 218, CRC Press, 01 Jan. 2006
English, In book

Bitter gourd seed fatty acid rich in 9c,11t,13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPAR gamma in human colon cancer Caco-2 cells
Y Yasui, M Hosokawa, T Sahara, R Suzuki, S Ohgiya, H Kohno, T Tanaka, K Miyashita
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 73, 2, 113, 119, Aug. 2005, [Peer-reviewed]
English, Scientific journal

Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues
H Maeda, M Hosokawa, T Sashima, K Funayama, K Miyashita
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 332, 2, 392, 397, Jul. 2005, [Peer-reviewed]
English, Scientific journal

Docosahexaenoic acid-containing phosphatidylethanolamine enhances HL-60 cell differentiation by regulation of c-jun and c-myc expression
H Ishigamori, M Hosokawa, H Kohno, T Tanaka, K Miyashita, K Takahashi
MOLECULAR AND CELLULAR BIOCHEMISTRY, 275, 1-2, 127, 133, Jul. 2005, [Peer-reviewed]
English, Scientific journal

Lipid peroxidation of a human hepatoma cell line (HepG2) after incorporation of linoleic acid, arachidonic acid, and docosahexaenoic acid
M Araseki, H Kobayashi, M Hosokawa, K Miyashita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 69, 3, 483, 490, Mar. 2005, [Peer-reviewed]
English, Scientific journal

Butyrate-Induced Growth Inhibition and Differentiation in Caco-2 Cells with Hybrid Liposomes
Zakir Hossain, Hideyuki Kurihara, Masashi Hosokawa, Koretaro Takahashi
Journal of Oleo Science, 54, 5, 281, 288, 2005, [Peer-reviewed]
English, Scientific journal

Araseki, M., Kobayashi, H., Hosokawa, M. and Miyashita. K.:"Lipid peroxidation of a human hepatoma cell line (HepG2) after incorporated linoleic acid, arachidonic acid, and docosahexaenoic acid", Biosci. Biotechnol. Biochem., 69:483-490(2005)*　　　　　　　　　　　　　　　
2005, [Peer-reviewed]

Ishigamori, H., Hosokawa, M., Kohno, H., Tanaka, T., Miyashita, K. and Takahashi, K.:"DHA-containing phosphatidylethanolamine enhances HL-60 cell differentiation through alterations of c-jun and c-myc expression", Mol. Cell. Biochem., 275:127-133(2005)*　　　　　　　　　　　　　　　
2005, [Peer-reviewed]

Growth inhibition and induction of differentiation and apoptosis mediated by sodium butyrate in Caco-2 cells with algal glycolipids
Z Hossain, H Kurihara, M Hosokawa, K Takahashi
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 41, 5-6, 154, 159, 2005, [Peer-reviewed]
English, Scientific journal

Synergistic Effects of Highly Unsaturated Fatty Acid-containing Phosphatidyl-ethanolamine on Differentiation of Human Leukemia HL-60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
Masashi Hosokawa
Japanese Journal of Cancer Research, 2005
Scientific journal

Growth inhibition of human pro-myelocytic leukemia (HL-60) cells by lipid extracts of marine alga Sargassum marginatum (Fucales, Phaeophyta) harvested off Goa (west coast of India) with special reference to fatty acid composition
N Bhaskar, M Hosokawa, K Miyashita
INDIAN JOURNAL OF MARINE SCIENCES, 33, 4, 355, 360, Dec. 2004, [Peer-reviewed]
English, Scientific journal

Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPAR gamma ligand, troglitazone, on colon cancer cells
Masashi Hosokawa
Biochimica et Biophysica Acta (BBA) - General Subjects, 1675, 1/3, 113, 119, Nov. 2004, [Peer-reviewed]
Scientific journal

Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARgamma ligand, troglitazone, on colon cancer cells.
Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K
Biochimica et biophysica acta, 1675, 1-3, 113, 119, Nov. 2004, [Peer-reviewed]

Dietary seed oil rich in conjugated linolenic acid from bitter melon inhibits azoxymethane-induced rat colon carcinogenesis through elevation of colonic PPAR gamma expression and alteration of lipid composition
H Kohno, Y Yasui, R Suzuki, M Hosokawa, K Miyashita, T Tanaka
INTERNATIONAL JOURNAL OF CANCER, 110, 6, 896, 901, Jul. 2004, [Peer-reviewed]
English, Scientific journal

Pomegranate seed oil rich in conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats
H Kohno, R Suzuki, Y Yasui, M Hosokawa, K Miyashita, T Tanaka
CANCER SCIENCE, 95, 6, 481, 486, Jun. 2004, [Peer-reviewed]
English, Scientific journal

Separation of sardine oil without heating from surimi waste and its effect on lipid metabolism in rats
K Toyoshima, R Noguchi, M Hosokawa, K Fukunaga, T Nishiyama, R Takahashi, K Miyashita
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 52, 8, 2372, 2375, Apr. 2004, [Peer-reviewed]
English, Scientific journal

Bioconversion of n-3 and n-6 PUFA by Clavibacter sp ALA2
M Hosokawa, CT Hou, D Weisleder
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 80, 11, 1085, 1091, Nov. 2003, [Peer-reviewed]
English, Scientific journal

Biosynthesis of tetrahydrofuranyl fatty acids from linoleic acid by Clavibacter sp ALA2
M Hosokawa, CT Hou, D Weisleder, W Brown
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 80, 2, 145, 149, Feb. 2003, [Peer-reviewed]
English, Scientific journal

Isolation of peptides with angiotensin I-converting enzyme inhibitory effect derived from hydrolysate of upstream chum salmon muscle
S Ono, M Hosokawa, K Miyashita, K Takahashi
JOURNAL OF FOOD SCIENCE, 68, 5, 1611, 1614, 2003, [Peer-reviewed]
English, Scientific journal

Hosokawa, M., Hou, C.T., Weisleder, W. and Brown, W.:"Isolation and structure determination of 12,13,16-trihydroxy-9(Z)-octadecenoic acid produced from linoleic acid by Clavibacter sp. ALA2 and biosynthesis of tetrahydrofuranyl fatty acids", J. Am. Oi・・・　　　　　　　　　　　　　　　
2003, [Peer-reviewed]
Hosokawa, M., Hou, C.T., Weisleder, W. and Brown, W.:"Isolation and structure determination of 12,13,16-trihydroxy-9(Z)-octadecenoic acid produced from linoleic acid by Clavibacter sp. ALA2 and biosynthesis of tetrahydrofuranyl fatty acids", J. Am. Oil Chem. Soc., 80:145-149(2003)*

Hosokawa, M., Hou, C.T. and Weisleder, D.:"Bioconversion products from n-3 and n-6 polyunsaturated fatty acids by Clavibacter sp. ALA2", J. Am. Oil. Chem. Soc., 80:1085-1091(2003)*　　　　　　　　　　　　　　　
2003, [Peer-reviewed]

Production of novel tetrahydroxyfuranyl fatty acids from alpha-linolenic acid by Clavibacter sp strain ALA2
M Hosokawa, CT Hou, D Weisleder
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69, 7, 3868, 3873, 2003, [Peer-reviewed]
English, Scientific journal

Dual action of isoprenols from herbal medicines on both PPAR gamma and PPAR alpha in 3T3-L1 adipocytes and HepG2 hepatocytes
N Takahashi, T Kawada, T Goto, T Yamamoto, A Taimatsu, N Matsui, K Kimura, M Saito, M Hosokawa, K Miyashita, T Fushiki
FEBS LETTERS, 514, 2-3, 315, 322, Mar. 2002, [Peer-reviewed]
English, Scientific journal

Takahashi, N., Kawada, T., Gotoh, T., Yamamoto, T., Taimatsu, A., Matsui, N., Kimura, K., Saitoh, M. Hosokawa, M., Miyashita, K. and Fushiki, T. "Dual action of isoprenols from herbal medicines on both PPAR and PPAR g in 3T3-L1 adipocytes and HepG2 he・・・　　　　　　　　　　　　　　　
2002, [Peer-reviewed]
Takahashi, N., Kawada, T., Gotoh, T., Yamamoto, T., Taimatsu, A., Matsui, N., Kimura, K., Saitoh, M. Hosokawa, M., Miyashita, K. and Fushiki, T. "Dual action of isoprenols from herbal medicines on both PPAR and PPAR g in 3T3-L1 adipocytes and HepG2 hepatocytes". FEBS Lett., 514: 315-322(2002) *

Proteolytic conversion of squid hepatopancreas and upstream chum salmon muscle to angiotensin I-converting enzyme inhibitory peptides
S Ono, M Hosokawa, A Inoue, D Yamadda, K Takahashi
NIPPON SUISAN GAKKAISHI, 68, 2, 192, 196, 2002, [Peer-reviewed]
Japanese, Scientific journal

Dietary conjugated linolenic acid inhibits azoxymethane-induced colonic aberrant crypt foci in rats
H Kohno, R Suzuki, R Noguchi, M Hosokawa, K Miyashita, T Tanaka
JAPANESE JOURNAL OF CANCER RESEARCH, 93, 2, 133, 142, 2002, [Peer-reviewed]
English, Scientific journal

Liposomal sn-2 DHA inserted phospholipid decreases tumor size of fibrosarcoma
Akihito Fujimoto, Jin-Ichi Sasaki, Masashi Hosokawa, Koretaro Takahashi
Fisheries Science, 68, 1639, 1640, 2002, [Peer-reviewed]
English, Scientific journal

Dietary effects of bitter gourd oil on blood and liver lipids of rats
R Noguchi, Y Yasui, R Suzuki, M Hosokawa, K Fukunaga, K Miyashita
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 396, 2, 207, 212, Dec. 2001, [Peer-reviewed]
English, Scientific journal

Ligands for peroxisome proliferator-activated receptors alpha and gamma inhibit chemically induced colitis and formation of aberrant crypt foci in rats
T Tanaka, H Kohno, S Yoshitani, S Takashima, A Okumura, A Murakami, M Hosokawa
CANCER RESEARCH, 61, 6, 2424, 2428, Mar. 2001, [Peer-reviewed]
English, Scientific journal

Kohno, H., Yoshitani, S. Takashima, S., Okumura, A., Hosokawa, M. and Yamaguchi, M., Tanaka, T. : “Troglitazone, a ligand for peroxisome proliferator-activated receptor γ, inhibits chemically-induced aberrant cript foci in rats”, Jpn. J. Cancer Res. 92・・・　　　　　　　　　　　　　　　
2001, [Peer-reviewed]
Kohno, H., Yoshitani, S. Takashima, S., Okumura, A., Hosokawa, M. and Yamaguchi, M., Tanaka, T. : “Troglitazone, a ligand for peroxisome proliferator-activated receptor γ, inhibits chemically-induced aberrant cript foci in rats”, Jpn. J. Cancer Res. 92: 396-403 (2001)

Synergistic effects of highly unsaturated fatty acid-containing phosphatidylethanolamine on differentiation of human leukemia HL-60 cells by dibutyryl cyclic adenosine monophosphate
M Hosokawa, A Sato, H Ishigamori, H Kohno, T Tanaka, K Takahashi
JAPANESE JOURNAL OF CANCER RESEARCH, 92, 6, 666, 672, 2001, [Peer-reviewed]
English, Scientific journal

Noguchi, R., Yasui, Y., Suzuki, R., Hosokawa, M., Fukunaga, K. and Miyashita, K.: “Dietary effect of bitter gourd oil on blood and liver lipids of rats”, Arch. Biochem. Biophys, 396: 207-212 (2001)　　　　　　　　　　　　　　　
2001, [Peer-reviewed]

Troglitazone, a ligand for peroxisome proliferator-activated receptor γ, inhibits chemically-induced aberrant crypt foci in rats
Hiroyuki Kohno, Shin-Ichiro Yoshitani, Shigeki Takashima, Ataru Okumura, Masashi Hosokawa, Nobuo Yamaguchi, Takuji Tanaka
Japanese Journal of Cancer Research, 92, 4, 396, 403, 2001, [Peer-reviewed]
English, Scientific journal

Production of tailor-made polyunsaturated phospholipids through bioconversions
K Takahashi, M Hosokawa
JOURNAL OF LIPOSOME RESEARCH, 11, 4, 343, 353, 2001, [Peer-reviewed]
English, Scientific journal

Hosokawa,M.,Shimatani,T.,Kaneda,T.,Inoue Y.and Takahashi K.:“Coversion to Docosahexaenoic Acid-Containing Phosphatidylserine from Squid Skin Lecithin by Phospholipase D-Mediated Transphosphatidylation”,Journal of Agricultural and Food Chemistry, 48:455・・・　　　　　　　　　　　　　　　
2000, [Peer-reviewed]
Hosokawa,M.,Shimatani,T.,Kaneda,T.,Inoue Y.and Takahashi K.:“Coversion to Docosahexaenoic Acid-Containing Phosphatidylserine from Squid Skin Lecithin by Phospholipase D-Mediated Transphosphatidylation”,Journal of Agricultural and Food Chemistry, 48:4550-4554(2000)※

Conversion to docosahexaenoic acid-containing phosphatidylserine from squid skin lecithin by phospholipase D-mediated transphosphatidylation
Masashi Hosokawa
Journal of Agricultural and Food Chemistry, 2000
Scientific journal

Antibacterial activity against cariogenic bacteria and inhibition of insoluble glucan production by free fatty acids obtained from dried Gloiopeltis furcata
H Kurihara, Y Goto, H Aida, M Hosokawa, K Takahashi
FISHERIES SCIENCE, 65, 1, 129, 132, Feb. 1999, [Peer-reviewed]
English, Scientific journal

Lipase-mediated Interesterification of a Highly Unsaturated Fatty Acid-containing Acylglycerol Mixture
HOSOKAWA Masashi, HORII Aki, TAKAHASHI Koretaro
Journal of Japan Oil Chemists' Society, 48, 1, 51, 53, Japan Oil Chemists' Society, 20 Jan. 1999, [Peer-reviewed]
Japanese, The triacylglycerol (TG) molar ratio was augmented by Lipozyme IM-mediated interesterification of docosahexaenoic acid (DHA) rich (72.0%) partial acylglycerol mixtures (DHA-70G). DHA-70G used as substrate contained TG at 49.3%. Through interesterification mediated by Lipozyme IM previously dried with phosphoric anhydride prior to reaction, TG increased to 64.5% by 24h.The addition of molecular sieves in the reaction system at this point was effective by further increased TG in the reaction product. This content finally reached 81.0% at 48h interesterification. DHA content dominated for 73.7...

Toda,H.,Kurihara,H.,Hosokawa,M. and Takahashi,K.:"Inhibition of α-glucuronidase by purine nucleotides",Fisheries Sci.,65:667-668(1999)*　　　　　　　　　　　　　　　
1999, [Peer-reviewed]

Hosokawa,M.,Wanezaki,S.,Miyauchi,K.,Kurihara,H.,Kohno,H.,Kawabata,J.,Odashima, S, and Takahashi,K.:"Apoptosis-inducing effect of fucoxanthin on human leukemia cell line HL-60",Food Sci.Technol.Res.,5:243-246(1999)*　　　　　　　　　　　　　　　
1999, [Peer-reviewed]

Differentiation-and Apoptosis-inducing Activities of Phospholipids Containing Docosahexaenoic Acid for Mouse Myeloid Leukemia M1 Cells
HOSOKAWA Masashi, MINAMI Keiichi, KOHNO Hiroyuki, TANAKA Yukihisa, HIBINO Hidehiko
Fisheries science : FS, 65, 5, 798, 799, 1999, [Peer-reviewed]
English

Inhibition of β-Glucuronidase by Purine Nucleotides
Masashi Hosokawa
Fisheries Science, 65, 4, 667, 668, 1999, [Peer-reviewed]
Scientific journal

Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60.
Masashi HOSOKAWA, Satoshi WANEZAKI, Kozue MIYAUCHI, Hideyuki KURIHARA, Hiroyuki KOHNO, Jun KAWABATA, Shizuo ODASHIMA, Koretaro TAKAHASHI
Food Science and Technology Research, 5, 3, 243, 246, Japanese Society for Food Science and Technology, 1999, [Peer-reviewed]
Scientific journal

Preparation of highly unsaturated fatty acid-containing phosphatidylcholine by transesterification with phospholipase A(2)
M Hosokawa, M Ito, K Takahashi
BIOTECHNOLOGY TECHNIQUES, 12, 8, 583, 586, Aug. 1998, [Peer-reviewed]
English, Scientific journal

Hosokawa,M.,Ito,M and Takahashi,K.:"Preparation of highly unsaturated fatty acid-containing phosphatidylcholine by transesterification with phospholipase A2",Biotechnol.Tech.,12:583-586(1998)*　　　　　　　　　　　　　　　
1998, [Peer-reviewed]

Increase in Deformability of Human Erythrocytes through the Action of β-Lysophospholipid Rich in n-3 Polyunsaturated Fatty Acid Content
HOSOKAWA Masashi, ONO Masayo, TAKAHASHI Koretaro, INOUE Yoshikazu
Journal of Japan Oil Chemists' Society, 47, 12, 1313, 1318,1360, Japan Oil Chemists' Society, 1998, [Peer-reviewed]
Lysophosphatidylcholine (LPC) that binds to docosahexaenoic acid (DHA) at the <I>sn</I>-2 position (β-DHA-LPC) was prepared by the Lipozyme mediated selective partial hydrolysis of DHA-enriched egg yolk lecithin. The β-DHA-LPC was found to be rapidly incorporated into erythrocyte cells and increase their deformability at 5μM/erythrocyte.

Water activity-adjusted enzymatic partial hydrolysis of phospholipids to concentrate polyunsaturated fatty acids
M Ono, M Hosokawa, Y Inoue, K Takahashi
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 74, 11, 1415, 1417, Nov. 1997, [Peer-reviewed]
English, Scientific journal

Effects of Phospholipids Containing Docosahexaenoic Acid on Differentiation and Growth of HL-60 Human Promyelocytic Leukemia Cells
TOCHIZAWA Kaoru, HOSOKAWA Masashi, KURIHARA Hideyuki, KOHNO Hiroyuki, ODASHIMA Shizuo, TAKAHASHI Koretaro
Journal of Japan Oil Chemists' Society, 46, 4, 383, 390,457, Japan Oil Chemists' Society, 1997, [Peer-reviewed]
English, Effects of docosahexaenoic acid (DHA) -containing phospholipids (DHA-PLs) on retionic acid (RA) -induced cell differentiation and growth inhibition of the human promyelocytic leukemia cell line, HL-60 were investigated. Cell differentiation judged from NBT reduction assay reached to 5060% by incubation with 12.5μM DHA-containing phosphatidylcholines (DHA-PCs) prior to the addition of RA. Differentiation without DHA-PCs remained at 39.2%. By prior incubation with DHA-containing phosphatidylethanolamines (DHA-PEs), differentiation was 70% at 12.5μM. DHA-PEs induced NBT reducing activity to as much as 2030%, and inhibited cell growth in the absence of RA. Activity for promoting cell differentiation differed according to DHA-PL molecular species. 1-Palmitoyl (or oleoyl)- 2-docosahexaenoyl-<I>sn</I>-glycero-3-phosphocholine (or phosphoethanolamine) was found most effective molecular species for enhancing RA-induced cell differentiation.

Ono, M., Hosokawa, M., Inoue, Y. and Takahashi, K. : "Water Activity Adjusted Enzymatic Partial Hydrolysis of Phospholipids to Concentrate Polyunsaturated Fatty Acids", J. Am. Oil Chem. Soc., 74 : 1415-1417 (1997)＊　　　　　　　　　　　　　　　
1997, [Peer-reviewed]

Ono, M., Hosokawa, M., Inoue, Y. and Takahashi, K. : "Concentration of Docosahexaenoic Acid-Containing Phospholipids through Lipozyme IM-Mediated Hydrolysis", J. Jpn. Oil Chem. Soc., 46 : 867-872 (1997)＊　　　　　　　　　　　　　　　
1997, [Peer-reviewed]

PREPARATION OF THERAPEUTIC PHOSPHOLIPIDS THROUGH PORCINE PANCREATIC PHOSPHOLIPASE A(2)-MEDIATED ESTERIFICATION AND LIPOZYME-MEDIATED ACIDOLYSIS
M HOSOKAWA, K TAKAHASHI, Y KIKUCHI, M HATANO
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 72, 11, 1287, 1291, Nov. 1995, [Peer-reviewed]
English, Scientific journal

APPLICATION OF WATER MIMICS ON PREPARATION OF EICOSAPENTAENOIC AND DOCOSAHEXAENOIC ACIDS CONTAINING GLYCEROLIPIDS
M HOSOKAWA, K TAKAHASHI, N MIYAZAKI, K OKAMURA, M HATANO
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 72, 4, 421, 425, Apr. 1995, [Peer-reviewed]
English, Scientific journal

EFFECT OF IN-VITRO TREATMENT WITH GLYCEROPHOSPHOLIPID CONTAINING HIGHLY POLYUNSATURATED FATTY-ACIDS ON DEFORMABILITY OF HUMAN ERYTHROCYTES
M NOJIMA, M HOSOKAWA, K TAKAHASHI, M HATANO, Y KIKUCHI
NIPPON SUISAN GAKKAISHI, 61, 2, 197, 203, Mar. 1995, [Peer-reviewed]
English, Scientific journal

EFFECT OF EPA AND DHA CONTAINING GLYCEROPHOSPHOLIPID MOLECULAR-SPECIES ON THE FLUIDITY OF ERYTHROCYTE CELL-MEMBRANES
M NOJIMA, M HOSOKAWA, K TAKAHASHI, M HATANO
FISHERIES SCIENCE, 60, 6, 729, 734, Dec. 1994, [Peer-reviewed]
English, Scientific journal

Phospholipase A2‐mediated synthesis of phosphatidylethanolamine containing highly unsaturated fatty acids
MASASHI HOSOKAWA, KORETARO TAKAHASHI, MUTSUO HATANO, MAKOTO EGI
International Journal of Food Science & Technology, 29, 6, 721, 725, 1994, [Peer-reviewed]
English, Scientific journal

SYNTHESIS OF PHOSPHATIDYLCHOLINE CONTAINING HIGHLY UNSATURATED FATTY-ACID BY PHOSPHOLIPASE-A2 AND EFFECT ON RETINOIC ACID-INDUCED DIFFERENTIATION OF HL-60 CELLS
M HOSOKAWA, H OHSHIMA, H KOHNO, K TAKAHASHI, M HATANO, S ODASHIMA
NIPPON SUISAN GAKKAISHI, 59, 2, 309, 314, Feb. 1993, [Peer-reviewed]
Japanese, Scientific journal

Polyunsaturated Fatty Phosphatide Synthesis by Industrial Phospholipase A2
HOSOKAWA Masashi, TAKAHASHI Koretaro, HATANO Mutsuo, EGI Makoto
Journal of the Japanese Society for Food Science and Technology, 38, 8, p695, 698, Japanese Society for Food Science and Technology, Aug. 1991, [Peer-reviewed]
Japanese, Phospholipids consist of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were synthesized by use of an industrial pancreatic phospholipase A₂. Lysophosphatidylcholine (LPC) isolated from a commercial emulsifier, mixed fatty acids obtained from sardine oil, the concentrated sardine oil (EPA+DHA>60%), and 99.4% EPA were used as substrates. Optimum water content was found to 13.4%/system, and optimum ratio of LPC to mixed free fatty acids was 11:18 in weight base. When the substrate contained free EPA+DHA over 68%, the obtained phosphatidylcholine (PC) contained more than 31% of EPA+DHA. And when the substrate was 99.4% EPA, the obtained PC contained more than 45% of EPA, indicating that more than 90% of EPA was incorporated into sn-2 position of the PC molecule.

MOLECULAR-SPECIES OF THE ENZYMATICALLY SYNTHESIZED POLYUNSATURATED FATTY-ACID RICH TRIGLYCERIDE
K OSADA, K TAKAHASHI, M HATANO, M HOSOKAWA
NIPPON SUISAN GAKKAISHI, 57, 1, 119, 125, 1991, [Peer-reviewed]
Japanese, Scientific journal

Fucoxanthinによる樹状細胞の炎症抑制およびミトコンドリア制御
七戸優斗, 高谷直己, 別府史章, 細川雅史, 日本水産学会大会講演要旨集(CD-ROM), 2024, 2024

北太平洋に分布する動物プランクトン群集の脂質特性分析
笹川拓真, 山口篤, 高谷直己, 細川雅史, 別府史章, 日本水産学会大会講演要旨集(CD-ROM), 2024, 2024

海洋細菌が産生する希少カロテノイドの同定と抗炎症活性評価
高谷直己, 別府史章, 細川雅史, 日本水産学会大会講演要旨集(CD-ROM), 2024, 2024

函館沿岸で採取したキングサーモンの脂質特性
李旻軒, 横山楓, 滑げい卓, 高谷直己, 別府史章, 細川雅史, 日本水産学会北海道支部大会講演要旨集, 2023 (CD-ROM), 2024

バキュロウィルスを用いた褐藻カロテノイド生合成関連酵素の機能実証
井上晶, 高谷直己, 細川雅史, 尾島孝男, マリンバイオテクノロジー学会大会講演要旨集, 23rd, 2023

海洋性カロテノイドastaxanthinの生体内代謝物の同定と組織分布
高谷直己, 向村奏, 別府史章, 細川雅史, 日本水産学会大会講演要旨集(CD-ROM), 2023, 2023

n-3PUFA結合ホスファチジルグリセロールの細胞内脂肪酸調節機構の解明
富樫咲, 別府史章, 高谷直己, 細川雅史, 日本水産学会大会講演要旨集(CD-ROM), 2023, 2023

昆虫細胞を用いた褐藻類β-カロテン水酸化酵素の同定
井上晶, 高谷直己, 細川雅史, 尾島孝男, 日本水産学会大会講演要旨集(CD-ROM), 2022, 2022

イトマキヒトデ脂質成分によるC2C12細胞の筋管形成促進効果
別府史章, 福島葵, 高谷直己, 細川雅史, 日本水産学会大会講演要旨集(CD-ROM), 2022, 2022

水産物由来アルキルグリセロールによるC2C12筋管サイズ増大効果
佐々木健朗, 亀山美宇, 高谷直己, 細川雅史, 別府史章, 日本栄養・食糧学会大会講演要旨集, 76th, 2022

海洋性カロテノイドfucoxanthinの新規生体内代謝物の同定と組織分布解析
高谷直己, 向村奏, 永田航大, 田谷大輔, 別府史章, 細川雅史, 日本栄養・食糧学会大会講演要旨集, 76th, 2022

糖尿病肥満モデルKK-A^yマウスにおける水産物由来モノアルキル型中性脂質の脂質代謝への影響
柿本真幸, 高谷直己, 細川雅史, 別府史章, 日本水産学会大会講演要旨集(CD-ROM), 2022, 2022

ドスイカ(Berryteuthis magister)由来モノアルキル型中性脂質の2型糖尿病モデルKK-A^yマウスに対する脂質代謝への影響評価
柿本真幸, 高谷直己, 細川雅史, 別府史章, 日本水産学会大会講演要旨集(CD-ROM), 2021, 2021

フコキサンチン生体内代謝物の合成および構造解析
向村奏, 永田航大, 高谷直己, 別府史章, 細川雅史, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2021, 2021

高度不飽和ホスファチジルグリセロールの酵素的合成と肝臓脂質低下作用
陳莉萍, 高谷直己, 別府文章, 宮下和夫, 細川雅史, 日本水産学会大会講演要旨集, 2020, 2020

非アルコール性脂肪肝炎誘導モデルマウスに対する海洋性カロテノイドfucoxanthinの炎症抑制作用
高谷直己, 河野友香, 別府史章, 宮下和夫, 細川雅史, 日本栄養・食糧学会大会講演要旨集, 74th, 2020

フコキサンチンによる非アルコール性脂肪肝炎抑制効果 (特集 脂質がつなぐもの : 生物工学的脂質研究が導く新しい学際研究と産業(後編))
高谷 直己, 細川 雅史, 生物工学会誌, 98, 10, 529, 532, 2020
日本生物工学会, Japanese

Furan fatty acidss-a new approach
Matt Miller, Donato Romanazzi, Masashi Hosokawa, Hajime Uchida, INFORM, 30, 25, 27, 01 Mar. 2019

n-3高度不飽和脂肪酸によるC2C12細胞の筋分化能保護効果
別府史章, 山崎凌平, 宮下和夫, 細川雅史, 日本栄養・食糧学会大会講演要旨集, 73rd, 2019

C2C12筋芽細胞におけるEPA/DHAの筋管形成能保護効果
別府史章, 山崎凌平, 宮下和夫, 細川雅史, 日本油化学会年会講演要旨集, 58th, 2019

ジヒドロキシ脂肪酸による糖尿病/肥満マウスの糖・脂質代謝改善作用
藤翔太, 別府史章, KIM Hak-Ryul, HOU Ching T., 宮下和夫, 細川雅史, 日本油化学会年会講演要旨集, 58th, 2019

アカモクからのMCT抽出油の機能性評価
松本卓己, 手良向一嘉, 別府史章, 細川雅史, 宮下和夫, 日本油化学会年会講演要旨集, 58th, 2019

フコキサンチンのUCP1欠損マウスにおけるミトコンドリア因子の発現誘導
秋田知輝, 岡松優子, 別府史章, 宮下和夫, 細川雅史, 日本油化学会年会講演要旨集, 58th, 2019

海洋性カロテノイドfucoxanthinの新規生体内開裂物による炎症抑制機構
高谷直己, 田谷大輔, 宮下和夫, 細川雅史, 日本栄養・食糧学会大会講演要旨集, 73rd, 2019

アスタキサンチン由来アポカロテノイドによる炎症抑制機構
高谷直己, 別府史章, 宮下和夫, 細川雅史, 日本油化学会年会講演要旨集, 58th, 2019

ウニ生殖巣投与がマウスの体重および肝臓の脂肪蓄積に及ぼす影響
山元涼子, 南久則, 松崎弘美, 坂下真実, 森田直樹, 西宮攻, 堤尚信, 細川雅史, 板橋豊, 松井利郎, 浦和寛, 日本食品科学工学会大会講演集, 65th, 2018

食品中のカロテノイド類
小泉次郎, 細川雅史, 農業および園芸, 92, 10, 875‐880, 880, 01 Oct. 2017, [Corresponding author]
養賢堂, Japanese

Fucoxanthin in the management of obesity and its related disorders
Kazuo Miyashita, Masashi Hosokawa, JOURNAL OF FUNCTIONAL FOODS, 36, 195, 202, Sep. 2017
English, Book review

Structure and functions of marine lipids
細川 雅史, Food style 21, 21, 6, 39, 44, Jun. 2017
食品化学新聞社, Japanese

食品因子による生活習慣病予防・改善機構の解明をめざして―3 褐藻由来フコキサンチンの抗肥満・抗糖尿病効果とその機序
西川翔, 細川雅史, 宮下和夫, 化学と生物, 54, 8, 580‐585, 585, 20 Jul. 2016
公益社団法人 日本農芸化学会, Japanese

海洋性カロテノイドの健康機能
細川 雅史, ニューフードインダストリー, 58, 6, 7, 12, Jun. 2016
食品資材研究会, Japanese

Biological importance of fucoxanthin and its metabolites
Kazuo Miyashita, Masashi Hosokawa, Nana Mikami, Yasuo Kokai, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 250, Aug. 2015, [Peer-reviewed]
English, Summary international conference

海洋細菌Jejuia pallidilutea 11shimoA1株の新規カロテノイド生合成系解析
高谷直己, 澤辺智雄, 宮下和夫, 細川雅史, マリンバイオテクノロジー学会大会講演要旨集, 17th, 2015

Anti-inflammatory effect of a novel phospholipid, phosphatidyl-panthenol
細川 雅史, コスメトロジー研究報告, 23, 111, 116, 2015
コスメトロジー研究振興財団, Japanese

ニュートリゲノミクス解析に基づく褐藻カロテノイド,フコキサンチンの栄養機能性 (特集 ニュートリゲノミクス : 学問分野としての成熟とこれから) -- (現在のニュートリゲノミクス研究)
宮下 和夫, 細川 雅史, 遺伝 : 生物の科学, 69, 1, 21, 27, Jan. 2015
エヌ・ティー・エス, Japanese

海洋細菌が有するカロテノイド合成系の体系的理解:フラボバクテリアにおけるmyxol合成系の特徴
中西誠人, 高谷直己, 高峰, 宮下和夫, 細川雅史, 大熊盛也, 大島健志朗, 服部正平, 澤辺智雄, 日本水産学会大会講演要旨集, 2014, 34, 19 Sep. 2014
Japanese

PPARγを活性化させる新規ニシン発酵食品の脂質解析
濱岡直裕, 中川良二, 太田智樹, 長島浩二, 細川雅史, 宮下和夫, 日本油化学会年会講演要旨集, 53rd, 86, 09 Sep. 2014
Japanese

Preventive Effects of Marine Carotenoids on Inflammation-related Diseases
細川 雅史, 微量栄養素研究, 31, 80, 87, 2014
日本微量栄養素学会事務局, Japanese

Promotion of UCP1 Expression by Brown Seaweed Carotenoid, Fucoxanthin
宮下 和夫, 細川 雅史, Bio industry, 30, 11, 31, 38, Nov. 2013
シーエムシー出版, Japanese

第二回日韓水産学会合同シンポジュウムに参加して
細川 雅史, 日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries, 79, 4, 744, 745, 15 Jul. 2013
The Japanese Society of Fisheries Science, Japanese

海洋細菌Jejuia pallidilutea A1株の新規カロテノイド合成とストレス応答
高谷直己, 西田健太郎, 澤辺智雄, 宮下和夫, 細川雅史, マリンバイオテクノロジー学会大会講演要旨集, 15th, 2013

Carotenoid Contents in Chlorella pyrenoidosa and Concentration by Saponification
加茂川 寛之, 細川 雅史, 阿部 真幸, 宮下 和夫, 北海道大学水産科学研究彙報, 62, 3, 83, 88, 14 Dec. 2012
Carotenoids are natural pigments found in plants, seaweeds, algae and bacteria. It is well know that carotenoids have physiological effects such as provitamin activity and antioxidative effect. Among carotenoids, -carotene, lutein and zeaxanthin are popular carotenoids in green leafy vegetables and green microalgae. Furthermore, violaxanthin and neoxanthin are also contained as minor carotenoids. These carotenoids are currently expected to exhibit health beneficial effects depend on their unique structure. However, there are only a few reports of quantitative analysis of neoxanthin and vio..., 北海道大学大学院水産科学研究院, Japanese

脂肪を燃やす二つの脂肪組織(バイオミディア)
細川 雅史, 生物工学会誌 : seibutsu-kogaku kaishi, 90, 11, 740, 740, 25 Nov. 2012
公益社団法人日本生物工学会, Japanese

脂肪を燃やす二つの脂肪組織
細川雅史, 生物工学会誌, 90, 11, 740, 740, 25 Nov. 2012
日本生物工学会, Japanese

Molecular mechanism of anti-obesity effect by fucoxanthin
HOSOKAWA MASASHI, 日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries, 78, 5, 1007, 1007, 15 Sep. 2012
Japanese

ヘマトコッカス藻由来アスタキサンチンの潰瘍性大腸炎予防効果
木澤有希, 山下栄次, 宮下和夫, 細川雅史, 日本水産学会大会講演要旨集, 2012, 74, 14 Sep. 2012
Japanese

高度不飽和脂肪酸含有糖脂質の酸化安定性に関する研究
山口智代, 細川雅史, 阿部真幸, 宮下和夫, 日本水産学会大会講演要旨集, 2012, 111, 26 Mar. 2012
Japanese

Functionalities of Brown Seaweed Lipids and its Application to Foods
宮下 和夫, 細川 雅史, Bio industry, 29, 2, 7, 14, Feb. 2012
シーエムシー出版, Japanese

褐藻由来フコキサンチンの白色脂肪組織におけるUCP1発現誘導機構
小出愛, 松本伊代, 別府史章, 前多隼人, 細川雅史, 宮下和夫, 日本農芸化学会大会講演要旨集(Web), 2012, 2012

I-5. Molecular mechanism of anti-obesity effect by fucoxanthin
Masashi Hosokawa, Nippon Suisan Gakkaishi (Japanese Edition), 78, 5, 1007, 1007, 2012
公益社団法人 日本水産学会, English

Study on phosphatidylation of functional compounds using phospholipases
山本 幸弘, 細川 雅史, 宮下 和夫, 成蹊大学理工学研究報告, 48, 2, 59, 62, Dec. 2011
Here I describe about the phosphatidylation of functional compounds using phospholipases which is covered with my doctoral thesis. Physiologically active compound such as conjugated fatty acid, terpenes and phenolic compounds sometime exert their activity not fully because of their poor solubility in a certain environment. On the other hand, phospholipids have characteristic amphiphilic property and biocompatibility. In this study, several physiologically active compounds were phosphatidylated using phospholipases such as phospholipase A2 and phospholipase D. The enzymatic reaction was modified and optimized for the yield. In addition, the phosphatidylated compounds were tested for their physiological activity in vitro. Conjugated linoleic acids which have anti-obesity effects were phosphatidylated mediated by phospholipase A2. In this reaction, it is fund that water mimics such as formamide was key factor for the synthetic reaction. Several terpenes and phenolic compounds which have anti-cancer effects or anti-oxidative effects were phosphatidylated mediated by phospholipase D. Some phosphatidylated compounds were exerted higher anti-cancer effects compare to parent compounds., 成蹊大学理工学部, Japanese

褐藻類に含まれる機能性n‐3系PUFAの酸化安定性
須田正剛, 阿部真幸, 細川雅史, 宮下和夫, 日本水産学会大会講演要旨集, 2011, 104, 28 Sep. 2011
Japanese

褐藻脂質中のフコキサンチン含有と脂肪酸組成の季節変動
植西洋平, 野村雅俊, 阿部真幸, 細川雅史, 宮下和夫, 安井肇, 日本水産学会大会講演要旨集, 2011, 104, 28 Sep. 2011
Japanese

渇藻カロテノイド,フコキサンチンの生体内抗酸化活性
AIRANTHI Widjaja‐Adhi, 岩崎さやか, 山口智代, 阿部真幸, 阿部真幸, 細川雅史, 宮下和夫, 日本食品科学工学会大会講演集, 58th, 91, 09 Sep. 2011
Japanese

Anti-obesity and anti-diabetic effects by marinechemical "fucoxanthin"
細川 雅史, Materials integration, 24, 8, 6, 10, Aug. 2011
ティー・アイ・シィー, Japanese

Preface
Ernesto M. Hernandez, Masashi Hosokawa, Omega-3 Oils: Applications in Functional Foods, v, 01 May 2011
Elsevier Inc., English, Others

Omega-3 Oils: Applications in Functional Foods
Ernesto M. Hernandez, Masashi Hosokawa, Omega-3 Oils: Applications in Functional Foods, 1, 305, 01 May 2011
Elsevier Inc., English, Others

Synergistic/Additive Health Effects of Fish Oil and Bio-Active Compounds
Tomoko Okada, Nana Mikami, Tomoyuki Okumura, Masashi Hosokawa, Kazuo Miyashita, Omega-3 Oils: Applications in Functional Foods, 177, 203, 01 May 2011
Elsevier Inc., English

2-I-29 フコキサンチンと活性型ビタミンD_3による複合的大腸がん細胞増殖抑制の作用解析(一般演題要旨,日本ビタミン学会第63回大会講演要旨)
寺崎 将, 長尾 昭彦, 田中 卓二, 細川 雅史, 宮下 和夫, 増田 園子, ビタミン, 85, 4, 250, 250, 25 Apr. 2011
日本ビタミン学会, Japanese

褐藻由来フコキサンチンによる肝臓でのSCD1発現抑制作用
別府史章, 篠田太郎, 細川雅史, 宮下和夫, 日本水産学会大会講演要旨集, 2011, 2011

フコキサンチンによる白色脂肪組織の肥大化抑制機構
細川雅史, 任美珍, 小出愛, 西川翔, 別府史章, 宮下和夫, 肥満研究, 17, Supplement, 2011

フコキサンチンの肥満初期における予防効果と抗炎症作用
任美珍, 別府史章, 宮下和夫, 細川雅史, 日本農芸化学会大会講演要旨集, 2011, 2011

ホタテ卵巣を用いた新規発酵食品の製造と脂質の酸化安定性に関する検討
濱岡直裕, 濱岡直裕, 太田智樹, 細川雅史, 宮下和夫, 日本食品科学工学会大会講演集, 58th, 2011

カロテノイド(8)フコキサンチン
細川 雅史, Functional food, 5, 1, 94, 98, 2011
フジメディカル出版, Japanese

2-I-16 活性型ビタミンD_3,Fucoxanthin,Shikoninによる複合的大腸がん細胞増殖抑制作用(一般演題,日本ビタミン学会第62回大会発表要旨)
寺崎 将, 高上馬 希重, 関崎 春雄, 長尾 明彦, 田中 卓二, 細川 雅史, 宮下 和夫, 増田 園子, ビタミン, 84, 4, 174, 174, 25 Apr. 2010
日本ビタミン学会, Japanese

HepG2におけるα-リノレン酸の代謝
津久井隆行, 細川雅史, 宮下和夫, 日本農芸化学会大会講演要旨集, 2010, 2010

フコキサンチンによるマクロファージおよびCD8+T細胞の白色脂肪組織への浸潤抑制作用
細川雅史, 任美珍, 別府史章, 宮下和夫, 肥満研究, 16, Supplement, 2010

アロニアエタノール抽出物の脂質代謝改善効果
梨田幸恵, 三上奈々, 細川雅史, 太田智樹, 宮下和夫, 日本食品科学工学会大会講演集, 57th, 2010

Future direction of lipid nutrition and development of functional foods: gift from seaweeds and its application
宮下 和夫, 細川 雅史, Journal of lipid nutrition, 19, 1, 39, 45, 2010
日本脂質栄養学会, Japanese

アスタキサンチンのデキストラン硫酸ナトリウム誘発マウス大腸炎に対する予防効果　　　　　　　　　　　　　　　
細川 雅史, 安井 由美子, 三瓶 雄司, 三上 奈々, 宮下 和夫, 田中 卓二, 日本栄養・食糧学会大会講演要旨集, 63回, 192, 192, May 2009
(公社)日本栄養・食糧学会, Japanese

海洋生命統御による食糧生産--北海道大学21世紀COEプログラム(4)新たな水産資源としての海藻の重要性
宮下 和夫, 細川 雅史, アクアネット, 11, 12, 78, 82, Dec. 2008
湊文社, Japanese

AGFD 62-The suppression effects of conjugated linolenic acids on chemically induced colon carcinogenesis.
Yumiko Yasui, Masashi Hosokawa, Kazuo Miyashita, Takuji Tanaka, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 236, Aug. 2008
English, Summary international conference

フコキサンチンの安全性評価
別府史章, 倉智麻木, 庭野吉己, 津久井隆行, 前多隼人, 細川雅史, 宮下和夫, 日本栄養・食糧学会大会講演要旨集, 62nd, 2008

内臓脂肪蓄積と食品機能成分:特にフコキサンチンについて (肥満と機能性食品)
前多 隼人, 細川 雅史, 宮下 和夫, Functional food, 1, 2, 159, 165, 2008
フジメディカル出版, Japanese

褐藻由来フコキサンチンのDHA増加作用
津久井隆行, 細川雅史, 宮下和夫, 生化学, 2007

ワカメ由来フコキサンチンの正常マウスに対する肝臓中のDHA増加効果
津久井隆行, 細川雅史, 宮下和夫, 日本油化学会年会講演要旨集, 46th, 2007

Chemopreventive effects of conjugated linolenic acids (CLN) occurring in plant seed oils
Bhaskar Narayan, Masashi Hosokawa, Yumiko Yasui, Rikako Suzuki, Hiroyuki Kohno, Takuji Tanaka, Kazuo Miyashita, Current Nutrition and Food Science, 3, 4, 309, 318, 2007
Bentham Science Publishers B.V., English, Book review

Study on Biofunctional Properties and Increased Utilization of Marine Lipids
HOSOKAWA Masashi, Oleoscience, 7, 12, 525, 532, 2007
Marine organisms contain carotenoids with unique structure, and highly unsaturated fatty acids (HUFA) with health beneficial effects. Fucoxanthin, from the edible seaweed <I>Undaria pinnatifida</I>, induced apoptosis in human leukemia and colon cancer cells. Furthermore, the activities of apoptosis induction by halocynthiaxanthin and fucoxanthinol isolated from the sea squirt <I>Halocynthia roretzi</I> as metabolites of fucoxanthin were higher than that of fucoxanthin. In addition, dietary fucoxanthin attenuated the weight gain of white adipose tissue and decreased blood glucose in obese/diabetic mice. These results show multi-functional properties of fucoxanthin and its metabolites. On the other hand, to extend the application of HUFAs, enzymatic preparation of phospholipid (PL) binding HUFA and bioconversion of HUFA to hydroxyl cyclic fatty acid have been attempted. Phospholipase A<SUB>2</SUB> and Lipozyme IM show specificities on <I>sn</I>-2 and <I>sn</I>-1 position of PL, respectively, in esterification and acidolysis. Utilization of water mimics was effective in increasing the reaction yield of HUFA-PL. Further, tetrahydrofuranyl hydroxyl and diepoxy bicyclic fatty acids were converted from HUFA by <I>Bacillus megateriurn</I> ALA2., Japan Oil Chemists' Society, Japanese

Study on biofunctional properties and increased utilization of marine lipids
細川 雅史, Oleoscience, 7, 12, 525, 532, 2007
日本油化学会, Japanese

Fucoxanthin, from edible seaweeds, and its multi-biological functions
Kazuo Miyashita, Masashi Hosokawa, Tokutake Sashima, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 232, 279, 279, Sep. 2006
English, Summary international conference

Physiological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) - A review
B Narayan, K Miyashita, M Hosokawa, FOOD REVIEWS INTERNATIONAL, 22, 3, 291, 307, Jul. 2006
English, Book review

北海道支部の活性化 : 支部大会での新たな試み(支部のページ)
細川 雅史, 澤谷 拓治, 山崎 浩司, 武田 浩郁, 天野 春菜, 日本水産学会誌, 71, 3, 385, 387, 15 May 2005
公益社団法人日本水産学会, Japanese

海藻由来フコキサンチンの脂肪細胞に対する分化調節機能と抗肥満効果 (特集:フィトケミカルの機能性(2))
前多 隼人, 細川 雅史, 宮下 和夫, 食品工業, 48, 8, 68, 74, 30 Apr. 2005
光琳, Japanese

海藻脂質の知られざる健康機能 : 褐藻フコキサンチンに脂肪蓄積抑制などの機能
細川 雅史, 宮下 和夫, 化学と生物, 43, 3, 150, 152, 01 Mar. 2005
日本農芸化学会, Japanese

9 trans,11 trans型CLAのヒト結腸ガン細胞に対する
別府史章, 細川雅史, 田中礼央, 宮下和夫, 日本農芸化学会大会講演要旨集, 2005, 2005

「がん予防食品開発の新展開 －予防医学におけるバイオマーカーの評価システム－」<大澤俊彦編>　　　　　　　　　　　　　　　
シーエムシー出版, 2005

Yasui, Y., Hosokawa, M., Kohno, H., Tanaka, T. and Miyashita, K.:" Chemopreventive effect of bitter gpourd seed oil rich in conjugated linolenic acid", Chapter 3, 30-41 (Hung, Y.S., Yanagita, T. and Knapp, H.R., AOCS Press, Champain, IL, USA)(2005)　　　　　　　　　　　　　　　
2005

Hosokawa, M. and Takahashi, K.:"Preparation of polyunsaturated phospholipids and their functional properties", Chapter 13, 13.1-13.13 (Hou, C.T.:"Handbook of Industrial Biocatalysis", CRC Press Taylor & Francis, Boca Raton, FL, USA)(2005)　　　　　　　　　　　　　　　
2005

「油脂・脂質の基礎と応用－栄養・健康から工業まで－」<日本油化学会編>　　　　　　　　　　　　　　　
日本油化学会, 2005

Takahashi, K. and Hosokawa, M.:"Functionalities and production with biocatalysis of two highly polyunsaturated phospholipids", Chapter 19, 274-284(Hung, Y.S., Yanagita, T. and Knapp, H.R., AOCS Press, Champain, IL, USA)(2005)　　　　　　　　　　　　　　　
2005

BIO R&D がん細胞を自滅させる海藻カロテノイド--フコキサンチンによるアポトーシス誘導
細川 雅史, バイオインダストリー, 21, 1, 52, 57, Jan. 2004
シーエムシー出版, Japanese

油化学辞典　　　　　　　　　　　　　　　
丸善, 2004

『水産食品栄養学-基礎からヒトへ-』<鈴木平光編>　　　　　　　　　　　　　　　
技報堂, 2004

ファインケミカルシリーズ『機能性脂質のフロンティア』<佐藤清隆・柳田晃良・和田俊 編>　　　　　　　　　　　　　　　
シーエムシー出版, 2004

水産学シリーズ142『水産機能性脂質』<高橋是太郎>　　　　　　　　　　　　　　　
恒星社厚生閣, 2004

Proteolytic conversion of late run chum salmon muscle to angiotensin I-converting enzyme inhibitory peptides with TermoaseR
Ono Seigo, Hosokawa Masashi, Miyasita Kazuo, Takahashi Koretarao, Bulletin of Fisheries Sciences, Hokkaido University, 54, 1, 1, 5, Jul. 2003
北海道大学, Japanese

ニガウリ種子油(BGO)によるazoxymethane(AOM)誘発ラット大腸発がん抑制
鈴木里加子, 甲野裕之, 山口かずえ, 細川雅史, 宮下和夫, 田中卓二, 日本癌学会総会記事, 62nd, 2003

Fujimoto, A., Sassaki, J., Hosokawa, M. and Takahashi, K.:"Fibrosarcoma may be suppressed by liposomal sn-2 DHA inserted phospolipid therapy", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Cha・・・　　　　　　　　　　　　　　　
2003
Fujimoto, A., Sassaki, J., Hosokawa, M. and Takahashi, K.:"Fibrosarcoma may be suppressed by liposomal sn-2 DHA inserted phospolipid therapy", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Champain (Y.S. Hung, S.J. Lin, P.C. Hung ed), (2003)pp. 290-295.

Hou, C.T., Hosokawa, M. and Iwasaki, Y.:"Bioconversion of unsaturated fatty acids to value-added functional products", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Champain (Y.S. Hung, S.J. L・・・　　　　　　　　　　　　　　　
2003
Hou, C.T., Hosokawa, M. and Iwasaki, Y.:"Bioconversion of unsaturated fatty acids to value-added functional products", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Champain (Y.S. Hung, S.J. Lin, P.C. Hung ed), (2003)pp. 12-17.

Hosokawa, M., Hou, C.T., Weisleder, D. and Brown, W."Formation of cyclic products from polyunsaturated fatty acids by Clavibacter sp. ALA2", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Champ・・・　　　　　　　　　　　　　　　
2003
Hosokawa, M., Hou, C.T., Weisleder, D. and Brown, W."Formation of cyclic products from polyunsaturated fatty acids by Clavibacter sp. ALA2", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Champain (Y.S. Hung, S.J. Lin, P.C. Hung ed), (2003)pp. 36-41.

Noguchi, R., Yasui, Y., Hosokawa, M., Fukunaga, K. and Miyashita, K.:"Bioconversion of CLN to CLA", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Champain (Y.S. Hung, S.J. Lin, P.C. Hung ed), ・・・　　　　　　　　　　　　　　　
2003
Noguchi, R., Yasui, Y., Hosokawa, M., Fukunaga, K. and Miyashita, K.:"Bioconversion of CLN to CLA", In Essential Fatty Acids and Eicosanoids Invited Paper from the Fifth International Congress, AOCS Press, Champain (Y.S. Hung, S.J. Lin, P.C. Hung ed), (2003)pp. 353-359.

Preparation of highly unsaturated phospholipid molecular species and functional properties
M Hosokawa, NIPPON SUISAN GAKKAISHI, 68, 4, 509, 512, Jul. 2002
Japanese, Book review

Molecular Structuring of Highly Polyunsaturated Phospholipids and Their Biological Functionalities
HOSOKAWA MASASHI, Bulletin of the Japanese Society of Scientific Fisheries, 68, 3, 297, 297, 15 May 2002
The Japanese Society of Fisheries Science, Japanese

Bioconversion of Highly Unsaturated Phospholipids and It's Biological Functions
HOSOKAWA Masashi, TAKAHASHI Koretaro, Oleoscience : Journal of Japan Oil Chemists' Society, 2, 1, 19, 25, 01 Jan. 2002
Biocatalytic approaches were rational ways to prepare and modify highly unsaturated phospholipids (HUFA-PL). The prepared HUFA-PL showed an antitumor effect in vivo, a promotional effect on cell differentiation, and an increase in deformability of erythrocytes <I>in Vitro</I>., 日本油化学会 = Japan Oil Chemists' Society, Japanese

高度不飽和リン脂質リポソームの性状とその抗腫瘍効果
藤本 章人, 佐々木 甚一, 細川 雅史, 高橋 是太郎, 脂質生化学研究, 43, 0, 318, 321, 10 May 2001
高度不飽和リン脂質は多様な機能を有する生体適合性(生体親和性)に優れた脂質形態である。本研究では高度不飽和リン脂質リポソームの免疫賦活促進機能を調べることを目的として、フィトグリコーゲン(PG)を内包させた高度不飽和リン脂質リポソーム及びPG未内包高度不飽和リン脂質リポソームを調製し、それらの性状と in vitro, in vivo 相方における抗腫瘍効果について検討した。調製したsn-2位DHA結合型リン脂質リポソーム(PC:PS=7:3 モル比)は6～9層からなるマルチラメラベシクル(MLV)であり、粒度分布のピークは約1 μ m付近にあった。PGを内包させなかったsn-2位DHA結合型リン脂質MLVは少なくとも1ヶ月以上の粒度分布安定性を示し、Meth-A fibrosarcomaに対して比較的強い抗腫瘍効果を示した。, 日本脂質生化学研究会, Japanese

植物種子油由来の共役型リノレン酸の細胞に対する致死作用
野口亮子, 鈴木里加子, 細川雅史, 宮下和夫, 阿部真幸, 日本農芸化学会誌, 75, 2001

第4版油化学便覧<油化学便覧編集委員会>　　　　　　　　　　　　　　　
丸善, 2001

Takahashi, K. and Hosokawa, M.: “Chapter 25 Production of tailor-made polyunsaturated phospholipids through bioconversions”, 517-526 (Kuo, T.M., Gardner, H.W.: "Lipid Biotechnology", MARCEL DEKKER, INC., New York) (2001)　　　　　　　　　　　　　　　
2001

DHA含有リン脂質の酵素変換と生理作用 (「リン脂質の生理作用」)
高橋 是太郎, 細川 雅史, 脂質栄養学, 9, 1, 19, 31, 2000
日本脂質栄養学会, Japanese

β-リゾ型高度不飽和リン脂質の調製及びヒト赤血球変形能改善効果
小野 雅代, 細川 雅史, 高橋 是太郎, 井上 良計, 脂質生化学研究, 40, 0, 204, 206, 02 Jul. 1998
Rhizomucor miehei 由来の固定化リパーゼ剤（リポザイムIM）の水分活性を中間水分活性域に制御することによってDHA強化鶏卵黄リン脂質を部分加水分解し、DHA含量が極めて高いβ-リゾ型リン脂質が得られた。このβ-リゾ型リン脂質は低濃度で速やかに赤血球に取り込まれ、赤血球の変形能を改善した。, 日本脂質生化学研究会, Japanese

Beneficial Effect of β-lysophospholipid Rich in Highly Unsaturated Fatty Acids on Deformability of Human Erythrocyte
小野 雅代, 細川 雅史, 高橋 是太郎, 井上 良計, ヘモレオロジー研究会誌, 1, 0, 7, 11, 1998
高度不飽和リン脂質には多彩な生理活性のあることが示唆され始めている。高度不飽和リン脂質は水産物に比較的豊富に含まれてはいるが，水産物より着臭や着色なしに抽出することは一般に難しい。一方，ドコサヘキサエン酸(DHA)を強化した鶏卵黄リン脂質は，着臭や着色が殆どないという利点を有しているが，水産物由来のリン脂質よりもDHA含量が低いという難点があった。我々はこのような点を解決するため，リパーゼによる鶏卵黄リン脂質の部分加水分解反応を試み，DHAを高濃度に含むリン脂質の調製法を確立した。本研究では，このように調製したβーリゾ型高度不飽和リン脂質の生理機能を検討するため，ヒト赤血球変形能改善効果について人工毛細血管モデル(MC-FAN)を用いて評価した。Immobilized lipase (Lipozyme IM) mediated selective partial hydrolysis of docosahexaenoic acid (DHA) enriched egg yolk phospholipids (PL) were carried out. By keeping the water activity of lipase at an intermediate level， DHA was effectively enriched in acyl moieties of PL. This DHA-PL was dominant in β-lysophospholipids，and has rapidly improved the deformability of human erythrocytes when evaluated with artificial capillary model.β- lysophospholipids might be one of the most effective chemical forms to incorporate DHA into cells，accordingly., ヘモレオロジー研究会, Japanese

Concentration of Docosahexaenoic Acid-Contain-ing Phospholipid through Lipozyme IM-mediated Hydrolysis(共著)
ONO Masayo, HOSOKAWA Masashi, INOUE Yoshikazu, TAKAHASHI Koretaro, Journal of Japan Oil Chemists' Society, 46, 8, 867, 872,936, 1997
A selective partial hydrolyses of egg-yolk lecithin from fish-oil fed hens and squid-skin lecithin were carried out with immobilized Rhizomucor miehei lipase (Lipozyme IM) in n-hexane media in order to concentrate docosahexaenoic acid (DHA) -containing phospholipids. DHA were abundant in phospholipid fractions that had recovered from the partial hydrolysates than the corresponding substrates i.e. egg-yolk lecithin and squid lecithin. Thus, it was clear that DHA ester bonds were less susceptible against hydrolysis than other fatty acid ester bonds. Optimum water activity (a_w) for Lipozyme IM to concentrate DHA-containing phospholipids was a_w=0.44. Under this a_w, DHA showed to three fold increase compared to that of the original egg-yolk lecithin from fish-oil fed hens, after 8 h reaction at 40°C. Water activity-controlled partial hydrolysis with Lipozyme IM was borned out to be a useful method for concentrating functional phospholipids., Japan Oil Chemists' Society

生体におけるリン脂質の性状と機能性
細川, 雅史, 菅原, 達也, 機能油脂懇話会
建帛社, Oct. 2020, 9784767962153, vi, 141p, Japanese

水産生物機能性成分の研究論文概要集 : 水産生物機能性成分と研究動向
細川, 雅史
日本水産油脂協会, Jul. 2016, vi, 50p, Japanese

「第7章2 カロテノイドの吸収と代謝」、宮澤陽夫編、「食品機能性成分の吸収・代謝機構」　　　　　　　　　　　　　　　
細川雅史
シーエムシー出版、東京, 2013, p215-223

Chapter 15 Chemopreventive effects of astaxanthin on inflammatory bowel disease and inflammation-related colon carcinogenesis. Editor, O Sommerburg, W Siems, K Kraemer . “Carotenoids and vitamin A in translational medicine”.　　　　　　　　　　　　　　　
M. Hosokawa, Y. Yasui
Boca Raton, USA.., 2013, p293-308.

第6章1-7 脂溶性色素、日本油化学会編、「油脂・脂質・界面活性剤データブック「I編 油脂・脂質」　　　　　　　　　　　　　　　
細川雅史
丸善、東京, 2012, p228-234

Omega-3 oils : applications in functional foods　　　　　　　　　　　　　　　
Hernandez Ernesto M, 細川 雅史
AOCS Press, 2011, English

Omega-3 oils : applications in functional foods
Hernandez, Ernesto M., 細川, 雅史
AOCS Press, 2011, 9781893997820, v, 305 p., English

褐藻脂質中に含まれるフコキサンチンの抗肥満および抗糖尿病作用
細川, 雅史
日本水産油脂協会, 2010, 21p, Japanese

The Encyclopedia of Industrial Biotechnology　　　　　　　　　　　　　　　
John Wiley & Sons, Inc., 2010

Biocatalysis and Biomolecular Engineering　　　　　　　　　　　　　　　
John Wiley & Sons, Inc., 2010

Handobook of seafood quality, safety and health application.　　　　　　　　　　　　　　　
Wiley-Blackwell, 2010

油脂・脂質の基礎と応用−栄養・健康から工業まで−（改定第2版）　　　　　　　　　　　　　　　
日本油化学会, 2009

Nutrigenomics and proteomics in health and disease　　　　　　　　　　　　　　　
Wiley-Blackwell, 2009

油脂・脂質の基礎と応用－栄養・健康から工業まで－（改定第2版）　　　　　　　　　　　　　　　
日本油化学会, 2009

カロテノイドの科学と最新応用技術　　　　　　　　　　　　　　　
シーエムシー出版, 2009

Biocatalysis and Agricultural Biotechnology　　　　　　　　　　　　　　　
CRC Press Taylor & Francis, 2009

水産物由来の脂溶性成分による脂肪細胞のライフサイクル制御機能の解明
細川, 雅史
[北海道大学], Apr. 2008, 1冊, Japanese

Composition, functionality and potential applications of seaweed lipids.　　　　　　　　　　　　　　　
John Wiley & Sons, Inc, 2008

Enzymatic production of marine-derived protein hydrolysates and their bioactive peptides for use in foods and nutraceuticals.　　　　　　　　　　　　　　　
John Wiley & Sons, Inc, 2008

Antiobesity effect of fucoxanthin from edible seaweeds and its multibiological functions.　　　　　　　　　　　　　　　
ACS, 2008

Cancer: Disease Progression and Chemoprevention　　　　　　　　　　　　　　　
Research Signpost, 2007

Biocatalysis and biotechnology for functional foods and industrial products　　　　　　　　　　　　　　　
CRC Press Taylor & Francis, 2007

Marine Nutraceuticals and Functional Foods　　　　　　　　　　　　　　　
CRC Press Taylor & Francis, 2007

Cancer: Disease Progression and Chemoprevention　　　　　　　　　　　　　　　
Research Signpost, 2007

Biocatalysis and biotechnology for functional foods and industrial products　　　　　　　　　　　　　　　
CRC Press Taylor & Francis, 2007

Nutraceutical and Specialty Lipids and their Co-Products　　　　　　　　　　　　　　　
CRC Press Taylor & Francis, 2006

Nutraceutical and Specialty Lipids and their Co-Products　　　　　　　　　　　　　　　
CRC Press Taylor & Francis, 2006

Molecular Nutrition　　　　　　　　　　　　　　　
Hokkaido University
Nov. 2020 - Present

栄養化学（2019年-）　　　　　　　　　　　　　　　
北海道大学
Apr. 2019 - Present

Biochemistry I　　　　　　　　　　　　　　　
Hokkaido University
Aug. 2018

生化学２（2018年）　　　　　　　　　　　　　　　
北海道大学

食品化学実験　　　　　　　　　　　　　　　
北海道大学

食品栄養学実験　　　　　　　　　　　　　　　
北海道大学

Research strategy for food ingredients that promote intracellular self-conversion of EPA/DHA from plant/seaweed-based n-3 fatty acids
Grants-in-Aid for Scientific Research
30 Jun. 2023 - 31 Mar. 2026
細川 雅史
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, 23K18042

Functional regulation of immune cells in the metabolic disease prevention mechanism by brown seaweed fucoxanthin
Grants-in-Aid for Scientific Research
01 Apr. 2021 - 31 Mar. 2024
細川 雅史
2021年度の研究において、以下の研究成果を得た。
(1)フコキサンチンを投与した肥満マウスの脂肪組織では、これまで報告しているように活性化マクロファージマーカーのF4/80およびM1マクロファージマーカーのCD11cのmRNA発現量の低下が確認された。さらに、M1マーカー(CD11c)/M2マーカー(Arg1)比も低下したことから、フコキサンチンがマクロファージの脂肪組織への浸潤を抑制するとともに、極性変化にも関わることが推察された。一方、T細胞マーカーを解析した結果、CD8に加えCD25およびCD4のmRNA発現量が低下し、T細胞サブセットへの影響が示唆された。(2)非アルコール性脂肪肝炎を誘導したマウスの肝臓では、フコキサンチンの投与によりCD8およびCD25のmRNA発現量が低下傾向を示した。(3)肝細胞およびマクロファージ株をフコキサンチン代謝物のフコキサンチノールで処理し、各オルガネラにおける蓄積をHPLCにて定量した。細胞膜、ミトコンドリア、ミクロソーム画分でフコキサンチノールが検出されたことから、細胞膜を通過しオルガネラへの移行することが推察された。特に、ミトコンドリア画分での蓄積が多いことが示唆された。(4)フコキサンチノールがミトコンドリア画分に蓄積していた点に着目し、ミトコンドリア機能に関わるミトコンドリアDNA量の測定を行った。NASHモデルマウスの肝臓からDNAを抽出し測定を行った結果、普通食群と比較してNASH誘導色群でみられたミトコンドリアDNA量の低下が、フコキサンチンにより抑制された。(5)エネルギー代謝に関わる白色脂肪組織から褐色脂肪組織への転換メカニズムとして分泌因子を介した細胞間コミュニケーションについて調べた。その結果、BMPを介した組織転換制御の可能性が認められた。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 21H02276

Enzymatic conversion of kelp biomass for biofuels and health beneficial products.
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2020 - 31 Mar. 2024
FOX BRIAN, 高須賀 太一, 細川 雅史, 栗原 秀幸
Our aim of the current project is to develop an efficient method to depolymerize natural kelps available in the Hokkaido ocean by a combination of several enzyme functions, including laminarin-degrading enzymes and alginate-degrading enzymes. A set of kelp spp. were obtained and ready to analytically evaluate their chemical compositions for the next fiscal year. Regarding a laminarin-degrading enzyme, we had picked up a glycoside hydrolase 55 enzyme (GH55) from a highly cellulolytic insect symbiont bacterium (Bianchetti and Takasuka et al., 2015, Journal of Biological Chemistry). 7 polysaccharide lyases 18 enzymes (PL18s), which function in alginate degradation, were selected from 7 different bacterial species, and four PL18s had been recombinantly expressed and subjected to the initial enzyme screening by using pure alginic acid substrate. Moreover, two polysaccharide lyase 17 enzymes (PL17s), were gene synthesized and cloned into the bacterial expression vector. From the previous studies, PL18 is shown to be the endo-type polysaccharide lyase, which produces oligoalginic acids (>3 degrees of polymerization (DP)), while PL17 should catalyze an exo-type reaction. In the FY2021, collected kelps will be tested to be hydrolyzed by at least three different enzymes, GH55, PL17, and PL18, and end products will be analyzed.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 20H02776

Developmental research of marine carotenoids on prevention of lifestyle-related diseases through their metabolite analysis and regulation of organ/cell interaction
Grants-in-Aid for Scientific Research
01 Apr. 2018 - 31 Mar. 2021
Hosokawa Masashi
A fucoxanthin-derived apocarotenoid, paracentrone was identified in liver and white adipose tissue of mice fed fucoxanthin. Further, dietary fucoxanthin attenuated hepatic fat accumulation and inflammation in the diet-induced nonalcoholic steatohepatitis model mice. Paracentrone suppressed the overexpression of inflammatory factors in activated RAW264.7 macrophages. Further, apo-10′-fucoxanthinal with an epoxide residue also suppressed pro-inflammatory cytokines through regulating IkB phosphorylation, while beta-apo-8′-carotenal did not suppress inflammation. In adipocytes and hepatocytes, paracentrone and apo-10′-fucoxanthinal downregulated the mRNA expression of proinflammatory mediators induced by the culture medium of activated RAW264.7 cells. These results suggest that apo-fucoxanthinoids derived from fucoxanthin regulate organ/cell interaction involved in chronic inflammation, which is basis of the development of lifestyle-related diseases.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 18H02274

Establishment of cold stress-resistance nori strains specialized for the mariculture in Tohoku in Japan
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2015 - 31 Mar. 2018
Mikami Koji
Tohoku area is the northern limit of the large scale-nori mariculture and has had a severe problem such as reduction of nori productivity in the early spring because of lowering temperature of the sea water, which affects negatively on seaweed growth. The present study therefore focuses on the establishment of a new strain enabling the sustainable production of nori in Tohoku area.
To establish cold stress-resistance strains, a strain whose growth tolerates to cold stress was first selected with the help from the Aichi Fisheries Research Institute. Next, the crossing of this strain with Kesen-nuna Susabi, generally used as a cultivation strain in Tohoku area, was performed and then several lines exhibiting characteristics different from both parental strains were finally isolated. Thus, it is necessary to confirm growth under cold stress conditions of these new strains, which could enable to establish novel strains that are adapting to mariculture in Tohoku area in near future.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 15H04539

Preventive effects on metabolic syndrome through adipose tissue browning induced by marine carotenoids
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2015 - 31 Mar. 2018
HOSOKAWA Masashi, KATSUKI Ami, OHUCHI Yuka, TYA Daisuke, TATEYAMA Riho, AKITA Tomoki
Fucoxanthin contained in brown seaweeds suppressed weight gain of white adipose tissue (WAT) through induction of mitochondrion factors including uncoupling protein 1 (UCP1) in adipose tissues. In diet-induce obese mice fed fucoxanthin, fatty acid oxidation was promoted. Induction of the brown adipocyte-like phenotype was also observed in C3H10T1/2 adipocytes treated with fucoxanthin metabolites. PGC-1alpha is suggested to be a critical factor to induce browning in white adipocytes by fucoxanthin. On the other hand, the number of progenitor cells to differentiate into beige adipocytes was demonstrated to decrease with aging.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 15H04545

Creation of A Light-Driven Marine Vibrio Factory for Biofuel Production
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
01 Apr. 2014 - 31 Mar. 2016
Sawabe Tomoo, HOSOKAWA MASASHI
An aim of the study is to develop light-driven marine vibrio biocatalysts in efficient biofuel production. We performed 1) gene expression of a Nonlabens sediminis proteorhdopsin gene, and 2) a cloning of a myxol synthetic gene cluster of Nonlabens ulvanivorans. We also accumulated fundamental knowledge on CRISPR/Cas system for further developments of a genome editing approach working in marine vibrio cells.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 26660168

Development of high perfomanced hydogen production system using marine vibrios as biocatalysts
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2013 - 31 Mar. 2016
Sawabe Tomoo, HOSOKAWA MASASHI, NAKAGAWA SATOSHI
Aims of the study are to establishment of 1) continuous culture system and 2) biorefinery of hydrogen producing marine vibrios as biocatalysts. We did succeeded 1) continuous hydrogen production for 70 days with appropriate hydrogen producing rates and yields, 2) alginate-hydrogen conversion using 2 kinds of biocatalysts, and 3) bioprospects of new and rare carotenoid producers and their genome information.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 25292122

海産軟体動物および藻類に含まれる脂溶性成分の抽出と特性分析および生活習慣病予防機能の評価
国際的な科学技術共同研究などの推進 国際科学技術共同研究推進事業 SICORP ニュージーランド
2015 - 2016
細川 雅史
(1) 本研究は、ニュージーランド産の軟体動物や海藻などの水産物、並びに藻類に含まれる脂溶性成分の特性を明らかにするとともに、抗肥満、抗糖尿病、炎症性腸疾患予防効果を見出すことを目的とする。更に、機能脂質成分の効率的な抽出法や強化養殖法について検討する。(2) 具体的には、ニュージーランド側が藻類や軟体動物から抽出物を得て、一部を日本側へ送付する。独自に確立した分析法により、日本側ではフラン酸や複合脂質、カロテノイド組成の分析と構造解析を行い、ニュージーランド側はオメガ-3脂肪酸やリゾルビン誘導体の分析を行う。日本側では、抽出物および分離した機能性脂質を用いて、細胞および動物実験によりそれらの抗肥満や抗糖尿病、炎症性腸疾患予防効果を評価する。一方、ニュージーランド側は機能性物質の効率的な抽出法や生産量の増加を目指して、水産軟体動物への飼料の開発やフォトバイオリアクターを用いた藻類養殖法の開発をはかる。(3) 本研究で日本とニュージーランドが交流を通じて、水産物中に含まれる脂質成分の新たな健康機能性の解明と産業利用にむけた生産法の開発に取り組む。これによって、両国を含め世界的に問題となっている生活習慣病予防へニュージーランド産水産物の利用が期待できるとともに、水産業の活性化につながる。更に、本プロジェクトでは日本側の若手研究者を含めた研究交流活動、ならびに両国のプロジェクト研究者に加え、関連する国内外の研究者を含めた研究セミナーを開催することで機能性食品と水産業の発展に向けた情報交換を積極的に進める。これによって、日本とニュージーランドの継続的な研究交流と共同研究体制を構築が期待される。
北海道大学, Principal investigator

Biological Localization and Functionality of Allenic Carotenoids
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2011 - 31 Mar. 2014
MIYASHITA Kazuo, HOSOKAWA Masashi
In the present study, molecular mechanisms were made clear for the anti-obesity effect of fucoxanthin by the induction of UCP1 in abdominal WAT. The anti-obesity effect of fucoxanthin was suggested to be due to its specific structure that contains two carotenoid end rings of the polyene chromophore containing allenic bond and OH and epoxy groups. Therefore, other allenic carotenoids or scission products from fucoxanthin could not show such activities as found in fucoxanthin. Fucoxanthin also improves insulin resistance and decreases blood glucose level through the regulation of adipocytokine secretions from the abdominal WAT. Another important molecular mechanism for the improvement of glucose utilization by fucoxanthin will be glucose transporter 4 (GLUT4) up-regulation and promotion of its translocation to cell membrane from cytosol.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23380068

Preventive effects of marine carotenoids on chronic inflammation-related diseases through regulation of cell and tissue communication
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2011 - 31 Mar. 2014
HOSOKAWA Masashi, YASUI Yumiko
Fucoxanthin (Fx) suppressed chronic inflammation in white adipose tissue by the inhibition of macrophage and T cell infiltration. Further, Fx down-regulated mRNA expression of chemokines and adipokines, which recruit immune cells and induce insulin resistance. In diabetic/obese mice fed Fx, GLUT4 activation was observed in skeletal muscle through the improvement of hyperglycemia. On the other hand, Fx and astaxanthin (Asx) extracted from H. pluvialis prevent DSS-induced colitis and colon carcinogenesis in mice. In colon of the mice fed Asx, mRNA expression levels of inflammatory cytokines tended to decrease compared to control mice. In addition, Asx down-regulated mRNA expression of inflammatory cytokines in macrophage-like cells and colon cells stimulated by the culture medium of activated macrophages. These results suggest that marine carotenoids prevent chronic inflammation and its-related diseases by the regulation of cell and tissue communication via chemokines and cytokines.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 23380120

Creation of marine vibrio cells with minimun gene sets for efficient biofuel production
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
2011 - 2013
SAWABE Tomoo, HOSOKAWA Masashi, NAKAGAWA Satoshi
In maintaining a sustainable ecosystem in this period of global warming, development of key technologies in the field of renewable energy sources has become an important challenge; a method of biofuel production from marine biomass could be one of the most crucial technologies in the future. In this study, we performed global transcriptome, fermentation product profiling, and metabolic engineering to design marine vibrio cells with efficient ethanol production from marine biomass.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 23658172

新規な生体親和性化粧品素材ホスファチジル-パンテノールの機能開発
産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 探索タイプ
2011 - 2012
細川 雅史
本事業では、酵素的合成法にて高収率での調製が可能になったホスファチジル-パンテノールの優れた抗炎症作用を評価することを目標とした。ホスファチジル-パンテノールは、活性化したマクロファージに対して炎症性サイトカインであるIL-6やIL-1β、TNFα、炎症性酵素であるCOX-2やiNOSのmRNA発現やタンパク質産生を抑制した。その効果は従来のパンテノールと比較して4-10倍以上高い活性であり、少量で抗炎症作用を発揮する優位性をもった新規物質であることが明らかとなった。特に、ホスファチジルパンテノールの優れた抗炎症作用に関わる分子機構として、NF-κBやAP-1など複数の炎症誘導因子に対して制御機能を示すことを見出し、新規化粧品素材としての有効性を実証した。
北海道大学

Regulation of carotenoid biosynthesis in non- photosynthetic bacteria by light irradiation
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
2011 - 2012
HOSOKAWA Masashi
11ShimoA1, which was isolated from a marine alga, synthesized a novel carotenoid,2’-isopentenyl saporxanthin (2’-isopentenyl Spx). Blue LED irradiation increased2’-isopentenyl Spx content in the culture medium of strain A1. Furthermore, blue LED also enhanced mRNA expression level of carotenoid synthetic genes such as crtB、crtI、crtY、crtZ. In addition, strain A1 has unique genes coded prenyltransferase and phytoene desaturase, which are suggested to play an important role for 2’-isopentenyl Spx synthesis in strain A1. Both genes also tended to be up-regulated by blue LED. These results show that strain A1 is a unique bacteria with carotenoid synthetic pathways regulated by blue light.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 23658155

Preventive effects of marine carotenoids on life-style-related diseases based on anti-inflammatory effects as molecular targets
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2008 - 2010
HOSOKAWA Masashi, YASUI Yumiko
Astaxanthin exhibited inhibitory effects on dextran sodium sulfate-induced colitis and inflammatory-related colon carcinogenesis in mice. In colon of the mice fed astaxanthin, mRNA expression levels of inflammatory cytokines such as TNF-α and IL-1β were suppressed through inhibition of NF-κB activation. After feeding for 20 weeks, colonic mucosal ulcers, dysplastic crypts and colonic adenocarcinoma were inhibited by 200 ppm astaxanthin-containing diet. In addition, astaxanthin, pectenolone, alloxanthin and diatoxanthin isolated from marine organisms attenuated excessive mRNA expression of inflammatory cytokines and COX-2 and iNOS in macrophages activated by LPS. On the other hand, dietary 0.2% fucoxanthin markedly suppressed mRNA expression of inflammatory cytokines such as TNF-α and IL-6 to induce insulin resistance and inhibited macrophage infiltration in white adipose tissue through suppression of MCP-1 mRNA expression after 4 weeks feeding. Further, fucoxanthinol, which is one of fucoxanthin metabolites, also regulated cross talk of adipocytes and macrophages via palmitic acid and TNF-α. These results suggest that anti-inflammatory effects are molecular mechanism for anti-obesity and anti-diabetic effects of fucoxanthin.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 20380117

抗糖尿病作用を示すキサントフィルの簡易濃縮法の開発
産学が連携した研究開発成果の展開 研究成果展開事業 地域事業 地域イノベーション創出総合支援事業 シーズ発掘試験
2009 - 2009
細川 雅史
ワカメなどに含まれるキサントフィルは、血糖値改善効果を有し、抗糖尿病効果が期待される。本研究では、キサントフィルとその他の成分の溶媒への溶解性の差を利用した簡便な濃縮法を開発する。具体的には、水産物から抽出した脂溶性成分を、溶媒混合物に溶解して二層分離した後、一方からキサントフィル濃縮物を回収するという極めて簡便な方法である。これにより、10-30%以上まで濃縮したキサントフィル素材の調製法を開発する。
北海道大学, Principal investigator

Regulation of adipocytes differentiation by marine lipids
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2005 - 2007
HOSOKAWA Masashi
Obesity is now recognized as a world wide problem. It is multiple risk factors for the life style-related diseases such as diabetes, hypertension, hyperlipidemia, and cardiovascular disease. Visceral fat accumulation plays crucial roles in the development of these diseases. So, it is important to prevent the life style-related diseases through significantly reducing excess accumulation of visceral fat. Fucoxanthin is a major carotenoid found in edible seaweed such as Undaria pinnatifida and Hijikia fusiformis. Fucoxanthin and its metabolite, fucoxanthinol, inhibited intercellular lipid accumulation and suppressed glycerol-3-phosphate dehydrogenase activity during adipocyte differentiation of 3T3-L1 cells. In addition, in 3T3-L1 cells treated with fucoxanthin and fucoxanthinol, peroxisome proliferators-activated receptor (PPAR_γ), which regulates adipogenic gene expression, was down-regulated in a dose-dependent manner. N-3 Highly unsaturated fatty acid, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which are major fatty acids in fish oil, also suppressed adipocyte differentiation of 3T3-L1 cells by down regulation PPAR_γ And C/EBP_α. White adipose tissue weight of mice fed fucoxanthin was significantly lower than that of mice fed control diet. Uncoupling protein 1(UCP1), a key molecule for metabolic thermogenesis, was clearly found in WAT of mice fed fucoxanthin. The anti-obesity effect of fucoxanthin was enhanced by the combination with fish oil containing DHA and EPA
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 17580178

高い生体適合性と機能性をもつ新規共役脂肪酸リン脂質の合成法の開発と実用化研究
産学が連携した研究開発成果の展開 研究成果展開事業 地域事業 地域イノベーション創出総合支援事業 シーズ発掘試験
2006 - 2006
細川 雅史
本研究では、優れた生活習慣病予防機能を有し且つ幅広い用途での利用が期待できる共役型脂肪酸含有リン脂質の酵素的新規合成法の開発を試みる。特に、ホスホリパーゼ、リパーゼを利用した機能性リン脂質の合成反応に関して、?リン脂質の所望の位置への共役型脂肪酸の精密合成反応、?リン脂質の塩基構造をセリンやグリセロールに変換するリン酸基転移反応法について検討することで、本研究の実用化への見通しを得る。
北海道大学, Principal investigator

Development of Oxidative Stability and Bioavailability of Fatty Nutraceuticals Using Single Dispersed Micro/Nanosphere
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2004 - 2006
MIYASHITA Kazuo, HOSOKAWA Masashi
1.The oxidative stability of EPA and DHA in aqueous solutions was dramatically different from that in bulk phase. When the oxidative stabilities of six types of typical PUFA were compared in micelles, LA was the most susceptible to oxidation. In contrast, DHA and EPA were very stable. Oxidative stability of EPA and DHA in emulsion depended on the particle size and the interface. With decreasing particle size the stability of DHA and EPA increased. The reverse result was obtained in the oxidation of linoleate.
2.The oxidative stability of lipids in emulsion is strongly affected by altering the droplet size and the packing of the emulsifier and lipid molecules at the interface. Oxidative stability was compared among TGs dispersed with three kinds of sucrose esters having the same fatty acyl composition, the stability increased with increasing their hydrophile-lipophile balance (HLB) in both TGs, although these emulsifiers had no effect on the oxidative stability of SoyTG and DHATG in bulk phase.
3.Antioxidant activity also depended on the interface. With increasing the concentration of antioxidatns in interface layer the stability of lipids increased.
4.Micro/nanosphere containing functional lipid compounds can be stabilized by the regulation of interface. Moreover, it would be more acceptable to biological systems than other forms.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 16380082

核内転写因子の発現を制御する高度不飽和リン脂質に関する研究
科学研究費助成事業 若手研究(B)
2002 - 2003
細川 雅史
昨年度の研究において、DHA含有ホスファチジルエタノールアミン(DHA-PE)がdibutyrylcAMP(dbcAMP)によるヒト白血病HL-60細胞の分化誘導を顕著に亢進することを明らかにした。更にその過程で核内転写因子のAP-1を構成するc-Junタンパク質の発現増加を確認した。AP-1は、c-Jun/c-Junのホモダーマーまたはc-Jun/c-Fos、c-Jun/ATF-2のヘテロダイマーによって構成され、MAPキナーゼにより活性化されることで分化に関連する遺伝子を発現する。これらの結果を踏まえ、本年度は以下の点を明らかにした。
1.DHA-PEとdbcAMPを併用した場合c-Fosタンパク質並びにそのmRNAの強い発現がみられたが、dbcAMPの単独処理におけるc-Fos発現量との間に大きな差が見られなかった。よって、dbcAMPによる分化の誘導にはc-Fosおよびc-Junの双方が重要な役割を果しているが、DHA-PEとの併用効果に発現にはc-Junタンパクがキーレギュレーターとなっていることが示唆された。
2.DHA-PEとdbcAMPにより併用処理したHL-60細胞では、MAPキナーゼの一つであるJNKが培養初期において一過的に活性化された。これによってAP-1の転写活性も高められ、分化に関連する遺伝子の発現が亢進されることが示唆された。
3.DHAをリガンドとすることが知られている核内受容体のペルオキシソーム増殖薬応答性受容体(PPAR)γの発現への影響を調べたが、その発現への影響は認められなかった。
以上の結果から、DHA-PEは分化誘導物質であるdbcAMPと併用することにより、c-Junの発現亢進とJNKの活性化により核内転写因子AP-1の転写活性を高め、ヒト白血病HL-60細胞への分化誘導効果を亢進することが示唆された。
日本学術振興会, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 14760131

DHA結合型リン脂質の構造と白血病細胞の分化誘導機構に関する研究
科学研究費助成事業 奨励研究(A)
1997 - 1998
細川 雅史
1. DHA結合型リン脂質の分化誘導促進効果:(1)シロサケ筋肉,精巣及びマイカ肝膵臓から抽出した水産リン脂質が,ヒト白血病(HL-60)細胞に対するレチノイン酸やジブチリルcAMPなどの分化誘導剤の作用を増強する効果,すなわち分化誘導促進能を有することが明らかとなった。(2)マウス白血病(M1)細胞に対しては,DHA結合型リン脂質単独においても分化誘導能が認められた。(3)分化誘導促進効果は,DHAの遊離脂肪酸形態に比べリン脂質形態,特にPE形態において顕著なものであったことから,機能発現にはリン脂質形態が重要であると推察された。
2. 細胞膜への影響:TNBSクエンチング法並びに脂肪酸組成分析におけるDHA含有率の上昇から,DHA結合型リン脂質がHL-60細胞内に取り込まれていることを確認した。
3. 機能遺伝子発現への影響:DHA結合型PEは,レチノイン酸の分化誘導過程において重要な役割を果たすレチノイドXレセプタ-α(RXRα)のmRNA量を増加させることが示唆された。
4. 細胞内情報伝達酵素への影響:DHA結合型リン脂質の分化誘導促進作用がプロテインキナーゼ阻害剤により抑制されたことから,分化誘導促進能はプロテインキナーゼを介した作用であることが示唆された。
以上の結果から,水産リン脂質を含めたDHA結合型リン脂質が白血病細胞に対して分化誘導能及び分化誘導促進能を有することが明らかとなった。機能発現にはリン脂質の構造が重要であり,細胞に取り込まれた後,細胞内情報伝達酵素や核レセプターの発現を介して分化誘導剤の作用を促進することが推察された。
日本学術振興会, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 09760187

ガン細胞の増殖、分化に及ぼすDNA含有リン脂質の影響に関する研究
科学研究費助成事業 奨励研究(A)
1995 - 1995
細川 雅史
酸素反応により種々のドコサヘキサエン酸(DHA)含有リン脂質を調製し、ヒト前骨髄性白血病細胞(HL-60細胞)の増殖、分化に及ぼす影響について検討した。
1.種々のDHA含有ホスファチジルコリン(PC)分子種をHL-60細胞培養液に添加して、あらかじめ24時間培養することにより、レチノイン酸(RA)の細胞分化誘導能が増強された(細胞分化誘導促進効果)。その効果はDNA含有PC分子種により異なり、1-Pal,2-DHA-PC及び1-Ole,2-DHA-PCにおいて顕著であった。
2.DHA含有PCの細胞分化誘導促進効果は濃度依存的に上昇し、それに伴い細胞内のDHA含量の増加が見られた。DHAの結合していないdi-Pal-PC、di-Ole-PC及び大豆PCには促進効果が見られなかった。
3.DHA含有リン脂質の化学形態の影響について検討したところ、同一分子種ではPCに比べ、ポスファチジルエタノールアミン(PE)、ホスファチジルセリン(PS)の形態において細胞分化誘導能の増強作用が大きく、特に1-Pal,2-DHA-PE及び1-Ole,2-DHA-PEにおいて細胞分化誘導促進効果が顕著であった。更に、DHA含有PE自体にも分化誘導作用が見られた。
4.HL-60細胞の増殖に及ぼすDHA含有リン脂質分子種の影響についても検討を行ったところ、DHA含有PEの形態において増殖抑制効果が大きかった。
以上の結果より、DHA含有リン脂質がガン細胞の分化誘導促進能や増殖抑制能を有し、その効果がリン脂質の分子種や化学形態によって異なることが明らかとなった。特に、1-Pal,2-DHA-PE及び1-Ole,2-DHA-PEの効果が顕著であったことから、制ガン剤の減量とともに副作用の軽減への利用が期待される。
日本学術振興会, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 07760196

赤血球の変形能に及ぼす高度不飽和脂肪酸含有リン脂質の影響に関する研究
科学研究費助成事業 奨励研究(A)
1994 - 1994
細川 雅史
1.赤血球の変形能をシリコン単結晶基板マイクロチャンネルを用いて測定することにより、再現性の高い測定結果が得られた。
2.リパーゼやホスホリパーゼを用いて合成したEPA,DHA含有ホスファチジルコリン(PC)は、赤血球にとり込まれることによりその変形能を向上させることを証明した。
3.エタノールアミン結合リン脂質ではsn-2位にDHAを含むものが、セリン結合リン脂質ではsn-1位にEPAを含むものが赤血球の変態能を大きく向上させることも証明できた。
4.リン脂質クラス間を比較すると、エタノールアミン結合リン脂質はコリン結合リン脂質およびセリン結合リン脂質よりも、赤血球変形能を向上させる作用が強いことを認めた。特に、sn-2位にDHAを含むエタノールアミン結合リン脂質が赤血球の変形能を最も向上できることが示唆された。
5.蛍光偏光解消法により赤血球膜の流動性を測定したところ、膜の流動性向上は赤血球の変形能の向上とよく対応していた。
6.鮭卵より調製したEPA,DHA含有リン脂質においても赤血球変形能の向上作用がみられた。
7.リノール酸を主な脂肪酸とする大豆リン脂質は赤血球の変形能を改善しなかった。また、水素添加したリン脂質は赤血球の変形能を明かに低下させた。
以上の結果により、EPA,DHA含有リン脂質が赤血球の変形能を向上させ、その作用の強弱が分子種や脂質形態によって異なることが明かとなった。血栓症などの成人病予防が大きな課題となっている今日、EPA,DHA含有リン脂質を合成し、機能の一部を分子種レベルで明かにできたことは意義のあることと考える。
日本学術振興会, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 06760183

赤血球膜流動性におよぼす高度不飽和脂肪酸含有複合脂質の影響に関する研究
科学研究費助成事業 奨励研究(A)
1993 - 1993
細川 雅史
1.ブタ膵臓ホスホリパーゼA_2によるsn-2位にEPA,DHAを含むホスファチジルコリンの合成、LipozymeIM60によるsn-1位にEPAを含むホスファチジルコリンの合成、Streptomyces sp.由来のホスホリパーゼDによるこれらのホスファチジルコリンのホスファチジル基転移を行うことができきた。これによりホスファチジルコリンと同一の脂肪酸残基をもつ高度不飽和脂肪酸含有ホスファチジルエタノールアミンとホスファチジルセリンの合成物を得ることができた。
2.コリン結合リン脂質に比べアミノ基結合リン脂質のほうが赤血球により導入されやすいことを明らかにできた。
3.TNBSクエンチング法により、赤血球にとり込まれたリン脂質の40〜50%は膜タンパク質近傍に存在することを示すことができた。
4.高度不飽和脂肪酸含有ホスファチジルエタノールアミンはほとんど溶血性を示さないことがわかった。
5.水素添加したリン脂質は赤血球膜の流動性を明らかに低下させることを確認できた。
6.大豆リン脂質は赤血球膜の流動性を改善できないことがわかった。
7.合成したEPA,DHA含有リン脂質はいずれも赤血球膜流動性を改善する作用のあることが確認された。
8.コリン結合リン脂質、アミノ基結合リン脂質はいずれもEPA,DHAをsn-2位に含む混合形態の場合に最も高い赤血球膜流動性改善効果を示すことがわかった。
9.同一の脂肪酸残基の場合、コリン結合リン脂質はアミノ基結合リン脂質よりも赤血球膜の流動性改善効果が低いことをみとめた。
以上の結果より判断し、高度不飽和脂肪酸含有リン脂質は赤血球膜の流動性を高めて赤血球の変形能を向上させ、ひいては高血圧症や血栓症の予防や治療に役立つことも期待できることが明かとなった。
日本学術振興会, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 05760159

Enzymatic synthesis of phospholipid containing polyunsaturated fatty acid and its pysiological functionality
Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (C)
1992 - 1993
TAKAHASHI Koretaro, HOSOKAWA Masashi, HATANO Mutsuo
Synthesis of phospholipid containing polyunsaturated fatty acid were carried out.
1. Incorporation of polyunsaturated fatty acid into position sn^<-2> of phospholipid was possible by the use of an industrial pancreatic phospholipase A_2 mediated esterification. using lysophosphatidylcholine isolated from a commercial emulsifier and mixed fatty acids from sardine oil or high purity EPA.DHA.
Incorporation of polyunsaturated fatty acid into position sn^<-1> of phospholipid was also possible by the use of an industrial immobilized lipase e.g. Lipozyme mediated transesterification. using phosphatidylcholine isolated from soy lecithin and mixed fatty acids from sardine oil or high purity EPA.DHA.
Over the medium range water activity of the synthetic reactor promoted the incorporation of polyunsaturated fatty acid into phospholipid. but at the same time. unfavorable hydrolysis has also been proceeded. and as a result, it has decreased the recovery of phospholipid.
Under the low water activity condition of the reactor, the incorporation of polyunsaturated fatty acid into phospholipid was sluggish. However, the recovery of phospholipid was relatively high.
Precised control of water activity and the amount of essential water substitutes e.g. polyalcohol made it possible to achieve maximum incorporation rate of polyunsaturated fatty acid into phospholipid, and at the same time, made it possible to obtain high recovery of phospholipid.
The rate of retinoic acid induced differentiation of the human promyelocytic lcukemia cell line (HL-60) was promoted by growing HL-60 cells in FBN-PNM pretreated with cither EPA containing phosphatidylcholine or DHA containing phosphatidylcholine.
Japan Society for the Promotion of Science, Grant-in-Aid for General Scientific Research (C), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 04660218

Highly utilization of marine biofunctional materials　　　　　　　　　　　　　　　
Competitive research funding