Jin Shigeki

Faculty of Medicine　Social Medicine　Social MedicineLecturer
Last Updated :2025/06/07

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Research Field

  • Life sciences, Forensic medicine

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Papers

  • Postmortem contrast-enhanced computed tomography via direct large-vessel puncture
    Shogo Shimbashi, Akiko Takeuchi, Motoo Yoshimiya, Shigeki Jin, Kotaro Matoba, Hideki Hyodoh
    Legal Medicine, 69, 102448, 102448, Elsevier BV, Jul. 2024
    Scientific journal
  • Factors that hinder critical thinking and their resolution: Is active learning the key?
    Akiko Takeuchi, Shigeki Jin, Manabu Murakami, Kotaro Matoba
    Pakistan journal of medical sciences, 40, 6, 1313, 1314, Jul. 2024, [International Magazine]
    English, Scientific journal
  • 既存のクッシング症候群治療薬からオシロドロスタットへの切り替えによる血中ステロイドホルモンプロファイルの変化
    横関 恵, 亀田 啓, 宮 愛香, 野本 博司, 神 繁樹, 的場 光太郎, 中村 昭伸, 渥美 達也
    日本内分泌学会雑誌, 100, 1, 346, 346, (一社)日本内分泌学会, May 2024
    Japanese
  • 既存のステロイドホルモン合成酵素阻害薬からオシロドロスタットへの切り替えによる血中ステロイドホルモンプロファイルの変化
    横関 恵, 亀田 啓, 宮 愛香, 野本 博司, 神 繁樹, 的場 光太郎, 中村 昭伸, 渥美 達也
    日本内分泌学会雑誌, 99, 5, 1428, 1428, (一社)日本内分泌学会, Apr. 2024
    Japanese
  • The importance of early clinical exposure and interprofessional collaboration: Commonalities between Taiwan and Japan in the field of community medicine.
    Manabu Murakami, Akiko Takeuchi, Shigeki Jin, Kotaro Matoba
    Journal of the Chinese Medical Association : JCMA, 86, 11, 1037, 1038, 01 Nov. 2023, [International Magazine]
    English
  • Can the "Five Challenges" Overcome the Problem of "Reform Without Change" in Medical Education? Reexamining the "Hidden Curriculum".
    Manabu Murakami, Akiko Takeuchi, Shigeki Jin, Kotaro Matoba
    Acta medica portuguesa, 36, 9, 613, 614, 01 Sep. 2023, [International Magazine]
    English
  • db/dbマウス副腎におけるステロイドホルモン合成亢進機序DHCR24阻害薬の効果
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌, 99, 1, 392, 392, (一社)日本内分泌学会, May 2023
    Japanese
  • db/dbマウス副腎におけるステロイドホルモン合成亢進機序DHCR24阻害薬の効果　　　　　　　　　　　　　　　
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌, 99, 1, 392, 392, (一社)日本内分泌学会, May 2023
    Japanese
  • db/dbマウスにおける副腎ステロイドホルモン合成亢進に対するDHCR24阻害薬の効果と副腎内代謝変化の検討　　　　　　　　　　　　　　　
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本臨床分子医学会学術総会プログラム・抄録集, 58回, 59, 59, 日本臨床分子医学会, Apr. 2023
    Japanese
  • db/dbマウスにおける副腎ステロイドホルモン合成亢進に対するDHCR24阻害薬の効果と副腎内代謝変化の検討　　　　　　　　　　　　　　　
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本臨床分子医学会学術総会プログラム・抄録集, 58回, 59, 59, 日本臨床分子医学会, Apr. 2023
    Japanese
  • Objective Evaluation of Oral and Pharyngeal Areas in Autopsy Cases of Obstructive Sleep Apnea Syndrome via Postmortem CT.
    Akiko Takeuchi, Hideki Hyodoh, Shigeki Jin, Satoshi Tanaka, Manabu Murakami, Kazuyuki Minowa, Kotaro Matoba
    Current medical imaging, 28 Mar. 2023, [International Magazine]
    English, Scientific journal, BACKGROUND: Obstructive sleep apnea syndrome (OSAS) can cause sudden death during sleep. Previous findings have suggested that OSAS development is related to maxillofacial morphology. Evaluation of facial morphology can determine the risk of developing the disease, and establishing an objective method to assess the underlying etiology of OSAS-related death would be advantageous. OBJECTIVE: The objective of this study is to determine the key features of obstructive sleep apnea syndrome (OSAS) using postmortem oral and pharyngeal computed tomography (CT). METHODS: We retrospectively assessed autopsy cases of patients with (n=25) and without (n=25) OSAS-related death. We used oral and pharyngeal CT images to compare the oral and pharyngeal cavity volume (OPCV), oral and pharyngeal soft tissue volume (OPSV), oral and pharyngeal air space volume (OPAV), and OPAV to OPCV ratio (%air). Receiver operating curve (ROC) analysis was used to determine the accuracy of OSAS prediction. We assessed participants with body mass index (BMI) values within the normal range. RESULTS: Among the 50 subjects, we observed significant between-group differences in OPSV, OPAV, and % air, whereas there were significant between-group differences in OPSV and %air among 28 subjects with normal BMI values. Both comparisons suggested that OSAS-related death was associated with low %air and high OPSV values. CONCLUSION: The %air and OPSV are useful for assessing postmortem oropharyngeal CT images. OSAS-related sudden death is likely when %air and OPSV values are ≤20.1% and ≥127.2 ml, respectively. Among those with normal BMI values, % air and OPSV values of ≤22.8% and ≥111.5 ml, respectively, predict OSAS-related sudden death.
  • 肥満・2型糖尿病モデルマウスにおける副腎ステロイドホルモン合成亢進に対するDHCR24阻害薬の効果
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌, 98, 5, 1443, 1443, (一社)日本内分泌学会, Mar. 2023
    Japanese
  • 肥満・2型糖尿病モデルマウスにおける副腎ステロイドホルモン合成亢進に対するDHCR24阻害薬の効果
    上垣 里紗, 亀田 啓, 柴山 惟, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌, 98, 5, 1443, 1443, (一社)日本内分泌学会, Mar. 2023
    Japanese
  • Objective evaluation of chest findings in infants by postmortem computed tomography.
    Shogo Shimbashi, Rina Hayata, Kotaro Matoba, Atsuko Saito, Tomoko Matoba, Akiko Takeuchi, Shigeki Jin, Hideki Hyodoh
    Legal medicine (Tokyo, Japan), 60, 102178, 102178, Feb. 2023, [International Magazine]
    English, Scientific journal, PURPOSE: The purpose of this study was to review the findings of computed tomography (CT) performed early postmortem on infants and to clarify the postmortem CT lung findings that occur in the absence of abnormal histopathological findings. MATERIALS AND METHODS: From July 2016 to March 2022, 72 infants were autopsied with postmortem CT (41 boys 31 girls, aged 0-36 (mean 8.2) months). Autopsy and postmortem CT lung findings were compared with the causes of death identified by the autopsies, namely sudden infant death syndrome (n = 37), acute circulatory system disease (18), drowning (7), asphyxia (5), and dehydration/undernutrition (5). RESULTS: The %aerated lung volume (-700 HU or less) ranged from 0 % to 33 % (mean 1.5 %, median 0 %), being <1 % in 61 cases (84.7 %) and >3 % in 3/5 (60 %) of the dehydration/undernutrition group. The dehydration/undernutrition group showed significant preservation of lung field air content compared with the other causes of death groups (p < 0.05). Receiver characteristic curve analysis showed a cut off value of 0.8 % and area under the curve of 0.88806. The drowning group had significantly greater pleural cavity fluid retention than the other causes of death groups (p < 0.05). No correlation was found between postmortem interval and pleural cavity fluid retention. However, resuscitation time and pleural cavity fluid retention were correlated. CONCLUSION: Evaluation of CT values on postmortem lung fields of infants usually reveals a marked decrease in air content. When air content exceeds 0.8% on infant postmortem CT, dehydration/undernutrition should be considered in the differential diagnosis.
  • How Undergraduates Perceive Stress Factors: An exploratory study.
    Manabu Murakami, Akiko Takeuchi, Shigeki Jin, Kotaro Matoba
    Pakistan journal of medical sciences, 39, 4, 1219, 1220, 2023, [International Magazine]
    English, Scientific journal
  • Novel production of β-cryptoxanthin in haskap (Lonicera caerulea subsp. edulis) hybrids: Improvement of carotenoid biosynthesis by interspecific hybridization
    Ryohei Fujita, Shigeki Jin, Kotaro Matoba, Yoichiro Hoshino
    Scientia Horticulturae, 308, 111547, 111547, Elsevier BV, Jan. 2023, [Peer-reviewed]
    English, Scientific journal
  • 直腸穿孔の原因特定に死後CT検査が有用であった一剖検例　　　　　　　　　　　　　　　
    的場 光太郎, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 赤川 有希, 早田 璃菜, 真橋 尚吾, 兵頭 秀樹
    法医学の実際と研究, 65, 119, 123, 法医学談話会, Nov. 2022
    Japanese
  • 直腸穿孔の原因特定に死後CT検査が有用であった一剖検例　　　　　　　　　　　　　　　
    的場 光太郎, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 赤川 有希, 早田 璃菜, 真橋 尚吾, 兵頭 秀樹
    法医学の実際と研究, 65, 119, 123, 法医学談話会, Nov. 2022
    Japanese
  • メトホルミン中毒による重篤な乳酸アシドーシスに対して血液浄化療法を行い救命に成功した一例　　　　　　　　　　　　　　　
    江原 慶介, 大城 あき子, 俵 敏弘, 岡本 博之, 清水 隆文, 大西 新介, 森下 由香, 奈良 理, 早川 峰司, 神 繁樹, 的場 光太郎
    日本救急医学会雑誌, 33, 10, 793, 793, (一社)日本救急医学会, Oct. 2022
    Japanese
  • 塩素化されたTyrosineのLC-MS/MS分析法の検討
    神 繁樹, 的場 光太郎, 赤川 有希, 早川 璃菜, 魚住 諒
    JSBMS Letters, 47, Suppl., 78, 78, (一社)日本医用マススペクトル学会, Aug. 2022
    Japanese
  • Nonverbal communication skills: New-era education needs of younger generation medical students
    Manabu Murakami, Shigeki Jin, Akiko Takeuchi, Kotaro Matoba
    Pakistan Journal of Medical Sciences, 38, 6, 1724, 1725, Pakistan Journal of Medical Sciences, 27 Jun. 2022, [Peer-reviewed]
    English, Scientific journal, doi: https://doi.org/10.12669/pjms.38.6.6058 How to cite this:Murakami M, Jin S, Takeuchi A, Matoba K. Nonverbal communication skills: New-era education needs of younger generation medical students. Pak J Med Sci. 2022;38(6):---------. doi: https://doi.org/10.12669/pjms.38.6.6058 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Objective skull fracture evaluation by using the postmortem 3D-CT skull fracture score in fatal falls
    Hideki Hyodoh, Akiko Takeuchi, Kotaro Matoba, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Shigeki Jin
    Legal Medicine, 56, 102048, 102048, Elsevier BV, May 2022
    Scientific journal
  • The usefulness of measuring n-butyric acid concentration as a new indicator of blood decomposition in forensic autopsy.
    Kotaro Matoba, Manabu Murakami, Emi Fujita, Shigeki Jin, Ryosuke Ogasawara, Tomoko Matoba, Akiko Takeuchi, Sanae Haga, Michitaka Ozaki, Hideki Hyodoh
    Legal medicine (Tokyo, Japan), 57, 102071, 102071, 15 Apr. 2022, [International Magazine]
    English, Scientific journal, In forensic medicine, although various alcohols have been reported as indicators of decomposition in collected blood, no studies have examined short-chain fatty acids as indicators. In this study, the blood n-butyric acid concentration was quantified, and the association between n-butyric acid and decomposition was investigated to determine whether the detection of n-butyric acid could be a new indicator of decomposition. Among the forensic autopsies performed from 2016 to 2018 in our laboratory, the cases were divided into decomposed (n = 20) and non-decomposed (n = 20) groups based on macroscopic findings. Blood samples collected at the time of autopsy were derivatized with 3-nitrophenylhydrazine hydrochloride after solid-phase extraction. The n-butyric acid concentration was measured using liquid chromatography-tandem mass spectrometry. In addition, ethanol and n-propanol were measured using a gas chromatography-flame ionization detector. There was a significant difference (p < 0.01) in the concentrations of n-butyric acid between the decomposed and non-decomposed groups (0.343 ± 0.259 [0.030-0.973] and 0.003 ± 0.002 [0.001-0.007] mg/mL, respectively). In the decomposed group, n-butyric acid was detected at high concentrations, even in cases where n-propanol was low. These results suggest that n-butyric acid is more likely to be an indicator of blood decomposition than n-propanol.
  • Skills Required of Geriatricians: Why Medical Students Do Not Select Geriatrics as Their Career
    Manabu Murakami, Shigeki Jin, Akiko Takeuchi, Kotaro Matoba
    Acta Médica Portuguesa, 35, 3, 231, 231, Ordem dos Medicos, 02 Mar. 2022
    Scientific journal, N/a.
  • Detection of catechins using a fluorescent molecule and its application toward the evaluation of astringent intensity
    Nobuyuki Hayashi, Tomomi Ujihara, Shigeki Jin
    The Analyst, 147, 20, 4480, 4488, Royal Society of Chemistry (RSC), 2022, [Peer-reviewed]
    English, Scientific journal, It is possible to estimate astringent intensities of catechin solutions based on changes in the intensity of fluorescence emission.
  • 3-クロロチロシンの検出によって塩素ガス中毒死を診断した一剖検例　　　　　　　　　　　　　　　
    的場 光太郎, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 赤川 有希, 早田 璃菜, 兵頭 秀樹
    法医学の実際と研究, 64, 25, 29, 法医学談話会, Nov. 2021
    Japanese
  • Poly(ADP-ribose) polymerase (PARP) is critically involved in liver ischemia/reperfusion-injury
    S. Haga, A. Kanno, N. Morita, S. Jin, K. Matoba, T. Ozawa, M. Ozaki
    The Journal of Surgical Research, 270, 124, 138, Oct. 2021, [Peer-reviewed]
    English, Scientific journal
  • 高コルチゾール血症を呈したFamilial Dysalbuminemic Hyperthyroxinemiaの一例
    千葉 幸輝, 亀田 啓, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌, 97, 1, 225, 225, (一社)日本内分泌学会, Apr. 2021
    Japanese
  • クロバエ科の蛆が薬物検査に有用であった一剖検例　　　　　　　　　　　　　　　
    的場 光太郎, 奥谷 菜穂子, 神 繁樹, 齋藤 厚子, 的場 智子, 竹内 明子, 兵頭 秀樹
    法医学の実際と研究, 63, 103, 106, 法医学談話会, Nov. 2020
    Japanese
  • Sample preparation method with ultrafiltration for whole blood thiosulfate measurement
    Shigeki Jin, Manabu Murakami, Kotaro Matoba, Tomoko Matoba, Sanae Haga, Michitaka Ozaki, Akiko Takeuchi, Hideki Hyodoh
    Legal Medicine, 47, 101765, 101765, Elsevier BV, Nov. 2020, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal, Quantitative analysis of thiosulfate is useful for diagnosing hydrogen sulfide poisoning. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables more rapid and sensitive measurements than previous methodologies. As simple measurements of blood thiosulfate concentration are affected by the blood matrix, blood is used as the solvent to prepare the standard solution for calibration curve generation. Thus, a large amount of blood devoid of thiosulfate is required. We developed a preparation method by incorporating an ultrafiltration step to overcome this limitation and generate a calibration curve using a standard solution prepared with pure water. We used this improved method to investigate the stability of thiosulfate in refrigerated samples. To compare the effects of refrigeration, blood samples were prepared using the following two methods: one sample was treated with a 50-kDa exclusion ultrafiltration membrane and the other was not treated. The samples were stored at 4 °C, and then measured at 0, 3, 6, 24, 48, and 96 h. The incorporation of the ultrafiltration step in the measurement procedure enabled the quantification of thiosulfate, by plotting a calibration curve using a standard of pure water; it did not require a blood standard. Additionally, the reduction in whole blood thiosulfate concentration was within 10% during 2 days of refrigeration. Thus, the need for a large amount of blood to prepare the standard solution was resolved by the ultrafiltration step in test sample preparation. This method is useful to measure thiosulfate concentration and is not hindered by sample refrigeration for a few days.
  • Comparison of anthocyanin distribution in berries of Haskap (Lonicera caerulea subsp. edulis (Turcz. ex. Herder) Hultén), Miyama-uguisukagura (Lonicera gracilipes Miq.), and their interspecific hybrid using imaging mass spectrometry
    Ryohei Fujita, Takahiro Hayasaka, Shigeki Jin, Shu-Ping Hui, Yoichiro Hoshino
    Plant Science, 300, 110633, 110633, Elsevier BV, Nov. 2020, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Haskap (Lonicera caerulea subsp. edulis), a shrub with violet-blue fruits, is distributed mainly in Hokkaido, Japan. Miyama-uguisukagura (Lonicera gracilipes), a species related to Haskap, produces red fruits. Interspecific hybridization of Miyama-uguisukagura and Haskap was performed to introduce novel characteristics in the resulting hybrids. The shape and color of the interspecific hybrid fruits differed from those of the parent fruits. A comparison of anthocyanin distribution among these three fruit types by imaging mass spectrometry (IMS) revealed the presence of five different anthocyanins. The average cyanidin 3,5-diglucoside and peonidin 3,5-diglucoside intensities in the interspecific hybrid fruit were higher than those of the parent fruits, whereas the average pelargonidin 3-glucoside, cyanidin 3-glucoside, and peonidin 3-glucoside intensities were the highest in Haskap. All anthocyanins were mainly accumulated in the inner and outer skins of Haskap and interspecific hybrid fruits, and in the skin of Miyama-uguisukagura fruits. The order of signal intensities of all anthocyanins among the three fruits was unchanged in different regions. Additionally, a comparison of IMS and LC/MS data from our previous study confirmed the possibility of comparing multiple fruits in the same plate by IMS. Thus, we elucidated anthocyanin distribution patterns of the interspecific hybrid and parent fruits by IMS.
  • 血中チオ硫酸塩濃度の保存による変化について　　　　　　　　　　　　　　　
    神 繁樹, 的場 光太郎, 的場 智子, 兵頭 秀樹
    日本法医学雑誌, 74, 1, 73, 73, (NPO)日本法医学会, Aug. 2020
    Japanese
  • クロバエ科の蛆が薬物検査に有用であった一剖検例　　　　　　　　　　　　　　　
    的場 光太郎, 奥谷 菜穂子, 神 繁樹, 兵頭 秀樹
    日本法医学雑誌, 74, 1, 90, 90, (NPO)日本法医学会, Aug. 2020
    Japanese
  • 高コルチゾール血症を呈したFamilial Dysalbuminemic Hyperthyroxinemiaの一例
    千葉 幸輝, 亀田 啓, 野本 博司, 曹 圭龍, 中村 昭伸, 神 繁樹, 的場 光太郎, 三好 秀明, 渥美 達也
    日本内分泌学会雑誌, 96, 1, 366, 366, (一社)日本内分泌学会, Aug. 2020
    Japanese
  • Analysis of steryl glucosides in rice bran-based fermented food by LC/ESI-MS/MS
    Tsuyoshi Murai, Shigeki Jin, Mari Itoh, Yukiko Horie, Tatsuya Higashi, Shigeo Ikegawa
    Steroids, 158, 108605, 108605, Elsevier BV, Jun. 2020, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Steryl glucosides (SGs) and acylated steryl glucosides (ASGs) are phytochemicals found in plant-based foods and are known as bioactive compounds with potential health benefits. These include anti-inflammatory properties, anti-diabetic effects, and modulation of immunoregulatory functions as well as having cholesterol lowering effects. In this study, three major SGs, i.e., glucosides of β-sitosterol, stigmasterol, and campesterol, were synthesized and used as standards for measurement of their contents in rice bran (RB)-based fermented food (FBRA) utilizing Aspergillus oryzae and raw material (RM). The compounds were quantified using liquid chromatography/electrospray ionization-tandem mass spectrometry. It was found that β-sitosteryl glucoside was most abundant among the analyzed glucosides in both samples, and the contents of each SG in FBRA decreased about 35% from those of RM. In contrast to SGs, the contents of ASGs in FBRA increased 1.5-fold during the fermentation process as evidenced by an alkaline hydrolysis. The present results suggest that the FBRA might have greater beneficial effects than the RM, since ASGs have shown to have more potent cholesterol lowering effects and stronger anti-diabetic properties than SGs.
  • Post-mortem computed tomography in sudden death by asthma.
    Casey Smith, Kotaro Matoba, Hideki Hyodoh, Manabu Murakami, Atsuko Saito, Tomoko Matoba, Nahoko Okuya, Shigeki Jin
    Legal medicine (Tokyo, Japan), 44, 101694, 101694, 19 Mar. 2020, [Peer-reviewed], [International Magazine]
    English, Ground glass attenuation (GGA) of the lungs is a common finding of post-mortem computed tomography (PMCT) that is associated with pulmonary congestion and edema due to cardiac failure during the agonal period, or due to post-mortem hypostasis. However, hypo-attenuation of the lung is an atypical finding of PMCT, and is usually a consequence of hypovolemic states before death or postmortem body posture after death. Previous studies have shown a few differential diagnoses, such as hypothermia, massive hemorrhage, asphyxiation by hanging, and dehydration, for hypo-attenuation of the lung. This report presents the case of a woman who died suddenly because of an asthma attack. Our PMCT analysis demonstrated hypoattenuation of the lung in this case. We suggest fatal asthma as a differential diagnosis for the appearance of hypo-attenuation of lungs on PMCT.
  • The infusion effect in postmortem lung CT
    Hideki Hyodoh, Kotaro Matoba, Manabu Murakami, Atsuko Saito, Nahoko Okuya, Tomoko Matoba, Shigeki Jin
    Forensic Imaging, 20, 200367, 200367, Elsevier BV, Mar. 2020, [Peer-reviewed]
    English, Scientific journal
  • Evaluation of Fruit Anthocyanin Composition by LC/MS in Interspecific Hybrids Between Haskap (Lonicera caerulea subsp. edulis (Turcz. ex. Herder) Hultén) and Miyama-uguisukagura (Lonicera gracilipes Miq.)
    Ryohei Fujita, Shigeki Jin, Takahiro Hayasaka, Kotaro Matoba, Yoichiro Hoshino
    The Horticulture Journal, 89, 4, 343, 350, Japanese Society for Horticultural Science, 2020, [Peer-reviewed]
    English, Scientific journal
  • Extracts of bilberry (Vaccinium myrtillus L.) fruits improve liver steatosis and injury in mice by preventing lipid accumulation and cell death
    Sanae Haga, YiMin, Hikari Yamaki, Shigeki Jin, Tetsuya Sogon, Naoki Morita, Michitaka Ozaki
    Bioscience, Biotechnology, and Biochemistry, 83, 11, 2110, 2120, Oxford University Press (OUP), 02 Nov. 2019, [Peer-reviewed]
    English, Scientific journal, ABSTRACT
    Bilberry has been reported to have anti-oxidant and anti-inflammatory properties. We studied the effect of bilberry (Vaccinium myrtillus L.) fruits extracts (BEs) on the pathogenesis caused by lipid accumulation in fatty liver and non-alcoholic steatohepatitis (NASH). 5 μg/ml of BEs was enough to suppress lipid accumulation in the fatty liver model of the mouse hepatic AML12 cells. BEs increased cell viability and anti-oxidant capacity, presumably by activating (phosphorylating) Akt/STAT3 and inducing MnSOD/catalase. BEs also significantly reduced Rubicon and induced p62/SQSTM1, possibly contributing to reduce cellular lipids (lipophagy). When the mice were fed supplemented with BEs (5% or 10%, w/w), hepatic steatosis, injury, and hypercholesterolemia/hyperglycemia were significantly improved. Furthermore, histological and cytokine studies indicated that BEs possibly suppress hepatic inflammation (hepatitis) and fibrosis. Therefore, BEs improved liver steatosis and injury, and potentially suppress fibrosis by suppressing inflammatory response, which therefore may prevent the progression of fatty liver to NASH.
  • 溺死事例の死戦期に起きた食物誤嚥が解剖所見や検査結果に与える影響に関する検討　　　　　　　　　　　　　　　
    的場 光太郎, 兵頭 秀樹, 齋藤 厚子, 的場 智子, 奥谷 菜穂子, 竹内 明子, 邵 旻蓉, 神 繁樹
    法医学の実際と研究, 62, 15, 19, 法医学談話会, Nov. 2019
    Japanese
  • 死後造影CTに関する検討(Postmortem enhanced CT(PMeCT)　　　　　　　　　　　　　　　
    兵頭 秀樹, 的場 光太郎, 神 繁樹
    日本法医学雑誌, 73, 1, 73, 73, (NPO)日本法医学会, May 2019
    Japanese
  • 死後変化による腐敗が血液中のチオ硫酸濃度に与える影響について　　　　　　　　　　　　　　　
    的場 光太郎, 神 繁樹, 兵頭 秀樹
    日本法医学雑誌, 73, 1, 85, 85, (NPO)日本法医学会, May 2019
    Japanese
  • チオ硫酸塩測定における血液サンプルの前処理の検討　　　　　　　　　　　　　　　
    神 繁樹, 的場 光太郎, 兵頭 秀樹
    日本法医学雑誌, 73, 1, 86, 86, (NPO)日本法医学会, May 2019
    Japanese
  • 心筋トロポニンT濃度の死後変化の影響に関する検討　　　　　　　　　　　　　　　
    的場 光太郎, 兵頭 秀樹, 齋藤 厚子, 石田 理沙, 神 繁樹
    法医学の実際と研究, 61, 151, 154, 法医学談話会, Nov. 2018
    Japanese
  • Lung weight estimation with postmortem CT in forensic cases
    Kotaro Matoba, Hideki Hyodoh, Lisa Ishida, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Nahoko Okuya, Sahar Almansoori, Emi Fujita, Mayumi Yamase, Minyo Shao, Shigeki Jin
    Legal Medicine, 35, 61, 65, Elsevier BV, Nov. 2018, [Peer-reviewed]
    English, Scientific journal
  • Photo-Activatable Akt Probe: A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells.　　　　　　　　　　　　　　　
    Haga, Sanae, Ozawa, Takeaki, Morita, Naoki, Asano, Mami, Jin, Shigeki, Yimin, Ozaki, Michitaka
    Oncology Research, 26, 6, 467, 472, 2018, [Peer-reviewed]
  • 溺水時の死戦期の食物誤嚥が諸検査に与える影響に関する考察　　　　　　　　　　　　　　　
    的場 光太郎, 兵頭 秀樹, 馮 飛, 神 繁樹
    法医学の実際と研究, 60, 262, 263, 法医学談話会, Nov. 2017
    Japanese
  • Estimating normal lung weight measurement using postmortem CT in forensic cases
    Kotaro Matoba, Hideki Hyodoh, Manabu Murakami, Atsuko Saito, Tomoko Matoba, Lisa Ishida, Emi Fujita, Mayumi Yamase, Shigeki Jin
    Legal Medicine, 29, 77, 81, Elsevier BV, Nov. 2017, [Peer-reviewed]
    English, Scientific journal
  • 腐敗脳に対する凍結処置による肉眼的観察の実験的検討　　　　　　　　　　　　　　　
    兵頭 秀樹, 的場 光太郎, 村上 学, 的場 智子, 齊藤 厚子, 馮 飛, 神 繁樹
    日本法医学雑誌, 71, 1, 73, 73, (NPO)日本法医学会, May 2017
    Japanese
  • 法医解剖における腐敗した脳の肉眼的観察のための凍結処理法の開発　　　　　　　　　　　　　　　
    的場 光太郎, 兵頭 秀樹, 村上 学, 的場 智子, 齋藤 厚子, 馮 飛, 神 繁樹
    日本法医学雑誌, 71, 1, 73, 73, (NPO)日本法医学会, May 2017
    Japanese
  • Freezing preparation for macroscopic forensic investigation in putrefied brain
    Kotaro Matoba, Hideki Hyodoh, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Fei Feng, Shigeki Jin
    Legal Medicine, 26, 6, 10, Elsevier BV, May 2017, [Peer-reviewed]
    English, Scientific journal, Purpose: To evaluate the usefulness of the applied freezing technique in putrefied brain for macroscopic investigation. Materials and methods: From October 2015 to September 2016, first the brains of 10 cadavers (control group: male 6, female 4, age 20-80 (mean 61.5), postmortem intervals (PMI) 14-75 (mean 29.7) days) were inspected following the standard practice (without freezing preparation), and then with 10 cadavers (freezing group: male 7, female 3, age 41-88 (mean 60.4), PMI 7-75 (mean 29.2) days) the freezing technique was used before the autopsy. The cut brain was investigated, and the gray-white matter difference was evaluated macroscopically. Results: In the control group, the brain parenchyma leaked out like sludge in 5, and there was difficulty maintaining its structure in 7. The gray-white matter difference was well visible in 3, but hard to distinguish in 3, and the total scores ranged from 0 to 9 (mean 4.4) points. In the freezing group, the entire putrefied brain was extracted as a solid organ, the gray-white matter differences were well visible, and the total scores were 6.7-9 (8.3) points. The gray-white matter difference was preserved in the freezing group (p < 0.05). Conclusion: The freezing procedures to evaluate the putrefied brain have been successfully applied, and it could be statistically more useful in putrefied brain investigation than the ordinary procedure. Postmortem CT can be useful to evaluate not only the degree of brain putrefaction, but also the degree of brain parenchyma freezing.
  • Experimental evaluation of freezing preparation for the macroscopic inspection in putrefied brain
    Hideki Hyodoh, Kotaro Matoba, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Fei Feng, Shigeki Jin
    Legal Medicine, 24, 19, 23, Elsevier BV, Jan. 2017, [Peer-reviewed]
    English, Scientific journal
  • Liquid chromatography-tandem mass spectrometry method for the determination of thiosulfate in human blood and urine as an indicator of hydrogen sulfide poisoning
    Chikatoshi Maseda, Akira Hayakawa, Katsuhiro Okuda, Masaru Asari, Hiroki Tanaka, Hiromi Yamada, Shigeki Jin, Kie Horioka, Kotaro Matoba, Hiroshi Shiono, Kazuo Matsubara, Keiko Shimizu
    Legal Medicine, 24, 67, 74, Elsevier BV, Jan. 2017, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Being a stable metabolite of hydrogen sulfide, thiosulfate has been utilized as an index for hydrogen sulfide poisoning (HSP). Thiosulfate analysis is mainly performed using gas chromatography/mass spectrometry (GC-MS) due to its high sensitivity and specificity. The GC-MS analysis requires two-step derivatizations of thiosulfate, and the derivative is not stable in solution as it has a disulfide moiety. To resolve this stability issue, we developed a novel analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for monitoring the pentafluorobenzyl derivative of thiosulfate (the first reaction product of the GC-MS method) in this study. The established method exhibited high reproducibility despite being a more simplified and rapid procedure compare to the GC-MS method. Phenyl 4-hydroxybenzoate was used as an internal standard because 1,3,5-tribromobenzene which had been used in the GC-MS method was not suitable compound for LC-MS/MS with Electrospray ionization (ESI) negative detection. The linear regression of the peak area ratios versus concentrations was fitted over the concentration ranges of 0.5-250μM and 0.25-250μM in blood and urine, respectively. The validation results satisfied the acceptance criteria for intra- and inter-day accuracy and precision. Blood and urine samples from 12 suspected HSP cases were tested using this method. The thiosulfate concentration detected in the sample coincided well with that determined at the scene of each HSP accident.
  • Semiautomated Segmentation and Measurement of Cytoplasmic Vacuoles in a Neutrophil With General-Purpose Image Analysis Software
    Maki Mizukami, Misaki Yamada, Sayaka Fukui, Nao Fujimoto, Shigeru Yoshida, Sanae Kaga, Keiko Obata, Shigeki Jin, Keiko Miwa, Nobuo Masauzi
    Journal of Clinical Laboratory Analysis, 30, 6, 918, 923, Wiley, Nov. 2016, [Peer-reviewed]
    English, Scientific journal
  • Development for the measurement of serum thiosulfate using LC-MS/MS in forensic diagnosis of H2S poisoning
    Shigeki Jin, Hideki Hyodoh, Kotaro Matoba, Fei Feng, Akira Hayakawa, Katsuhiro Okuda, Keiko Shimizu, Sanae Haga, Michitaka Ozaki, Koichi Terazawa
    LEGAL MEDICINE, 22, 18, 22, Sep. 2016, [Peer-reviewed]
    English, Scientific journal
  • Highly selective recognition of tryptophan in water by a poorly water-soluble scandium compound
    Hayashi Nobuyuki, Jin Shigeki, Ujihara Tomomi
    TETRAHEDRON LETTERS, 56, 41, 5557, 5560, 07 Oct. 2015, [Peer-reviewed]
  • Anti-apoptotic effects of novel phenolic antioxidant isolated from the Pacific oyster (Crassostrea gigas) on cultured human hepatocytes under oxidative stress.
    Fuda H, Watanabe M, Hui SP, Joko S, Okabe H, Jin S, Takeda S, Miki E, Watanabe T, Chiba H, These authors contributed equally to the work
    Food Chemistry, 176, 226, 233, Jun. 2015, [Peer-reviewed]
  • Identification of molecular species of cholesteryl ester hydroperoxides in very low-density and intermediate-density lipoproteins.
    Shrestha R, Hui SP, Sakurai T, Yagi A, Takahashi Y, Takeda S, Jin S, Fuda H, Chiba H
    Annals of clinical biochemistry, 51, Pt 6, 662, 671, Nov. 2014, [Peer-reviewed]
    Impact Factor (2014): 2.335

    Cite Score (2014): 1.71
  • Evaluation of antioxidant activity of natural and synthetic compounds against LDL oxidation using CNT electrodes
    Ohkawa F, Takeda S, Hui SP, Sakurai T, Jin S, Fuda H, Chiba H
    IEEE Sensors J., 14, 2, 532, 537, Feb. 2014, [Peer-reviewed]
  • Recognition of caffeine by a water-soluble acyclic phane compound
    Hayashi Nobuyuki, Ujihara Tomomi, Jin Shigeki
    TETRAHEDRON, 70, 4, 845, 851, 28 Jan. 2014, [Peer-reviewed]
  • Measurement of single low-density lipoprotein particles by atomic force microscopy.
    Sakurai T, Takeda S, Takahashi JY, Takahashi Y, Wada N, Trirongjitmoah S, Namita T, Jin S, Ikuta A, Furumaki H, Hui SP, Fuda H, Fujikawa M, Shimizu K, Chiba H
    Annals of clinical biochemistry, 50, Pt 6, 564, 570, Nov. 2013, [Peer-reviewed]
    English, Scientific journal
  • Evaluation of oxidized low-density lipoproteins using Kelvin Force microscopy
    Takeda S, Ohkawa F, Hui SP, Sakurai T, Jin S, Fuda H, Sueoka K, Chiba H
    IEEE SENSORS J., 13, 9, 3449, 3453, Sep. 2013, [Peer-reviewed]
  • Immunological detection of large oxidized lipoproteins in hypertriglyceridemic serum.
    Sakurai T, Wada N, Takahashi Y, Ichikawa A, Ikuta A, Furumaki H, Hui SP, Jin S, Takeda S, Fuda H, Fujikawa M, Shimizu C, Nagasaka H, Furukawa H, Kobayashi S, Chiba H
    Annals of clinical biochemistry, 50, Pt 5, 465, 472, Sep. 2013, [Peer-reviewed]
    Impact Factor (2013): 2.080

    Cite Score (2013): 1.79
  • Quantification of urinary 18-hydroxycortisol using LC-MS/MS.
    Jin S, Wada N, Takahashi Y, Hui SP, Sakurai T, Fuda H, Takeda S, Fujikawa M, Yanagisawa K, Ikegawa S, Kurosawa T, Chiba H
    Annals of clinical biochemistry, 50, Pt 5, 450, 456, Sep. 2013, [Peer-reviewed]
    Impact Factor (2013): 2.080

    Cite Score (2013): 1.79
  • Analysis of triacylglycerol hydroperoxides in human lipoproteins by Orbitrap mass spectrometer
    Hui SP, Sakurai T, Takeda S, Jin S, Fuda H, Kurosawa T, Chiba H
    Anal Bioanal Chem, 405, 14, 4981, 4987, May 2013, [Peer-reviewed]
  • Evaluation of various electrode materials for detection of oxidized low-density lipoproteins
    Takeda S, Hui SP, Fuda H, Jin S, Sakurai T, Ishii A, Mukasa K, Sueoka K, Chiba H
    J Biomed Nanotechnol, 9, 2, 303, 306, Feb. 2013, [Peer-reviewed]
  • Effects of acid oxidation on carbon nanotube based electrodes for detection of oxidized LDL
    Seiji Takeda, Toshihiro Sakurai, Futaba Ohkawa, Shigeki Jin, Shu-Ping Hui, Hirotoshi Fuda, Koichi Mukasa, Hitoshi Chiba, Kazuhisa Sueoka
    2013 IEEE SENSORS, 1038, 1041, 2013, [Peer-reviewed]
    English, International conference proceedings
  • Removal of Acidic or Basic alpha-Amino Acids in Water by Poorly Water Soluble Scandium Complexes
    Nobuyuki Hayashi, Shigeki Jin, Tomomi Ujihara
    JOURNAL OF ORGANIC CHEMISTRY, 77, 21, 9652, 9658, Nov. 2012, [Peer-reviewed]
    English, Scientific journal
  • A phenolic antioxidant from the Pacific oyster (Crassostrea gigas) inhibits oxidation of cultured human hepatocytes mediated by diphenyl-1-pyrenylphosphine
    Mitsugu Watanabe, Hirotoshi Fuda, Shigeki Jin, Toshihiro Sakurai, Shu-Ping Hui, Seiji Takeda, Takayuki Watanabe, Takao Koike, Hitoshi Chiba
    FOOD CHEMISTRY, 134, 4, 2086, 2089, Oct. 2012, [Peer-reviewed]
    English, Scientific journal
  • LC‐MS/MSで測定した血清,尿18‐hydroxycortisolによる原発性アルドステロン症の診断
    和田典男, 神繁樹, 藤谷昴文, 惠淑萍, 柳澤克之, 黒澤隆夫, 千葉仁志
    日本内分泌学会雑誌, 88, 2, 695, 20 Sep. 2012
    Japanese
  • Novel monoclonal antibody recognizing triglyceride-rich oxidized LDLs associated with severe liver disease and small oxidized LDLs in normal subjects
    Toshihiro Sakurai, Ayako Ichikawa, Hiroyuki Furukawa, Norio Wada, Atsushi Nagasaka, Yuji Takahashi, Masato Fujikawa, Akiko Ikuta, Hiroaki Furumaki, Maiko Shiga, Chikara Shimizu, Shu-Ping Hui, Shigeki Jin, Seiji Takeda, Hirotoshi Fuda, Hironori Nagasaka, Seiichi Kobayashi, Hitoshi Chiba
    ANNALS OF CLINICAL BIOCHEMISTRY, 49, 5, 456, 462, Sep. 2012, [Peer-reviewed]
    English, Scientific journal
  • Detection and characterization of cholesteryl ester hydroperoxides in oxidized LDL and oxidized HDL by use of an Orbitrap mass spectrometer
    Shu-Ping Hui, Toshihiro Sakurai, Futaba Ohkawa, Hiroaki Furumaki, Shigeki Jin, Hirotoshi Fuda, Seiji Takeda, Takao Kurosawa, Hitoshi Chiba
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 404, 1, 101, 112, Jul. 2012, [Peer-reviewed]
    English, Scientific journal
  • Quantitative determination of phosphatidylcholine hydroperoxides during copper oxidation of LDL and HDL by liquid chromatography/mass spectrometry
    Shu-Ping Hui, Yudai Taguchi, Seiji Takeda, Futaba Ohkawa, Toshihiro Sakurai, Shinobu Yamaki, Shigeki Jin, Hirotoshi Fuda, Takao Kurosawa, Hitoshi Chiba
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 403, 7, 1831, 1840, Jun. 2012, [Peer-reviewed]
    English, Scientific journal
  • Detection of oxidized LDL using a carbon nanotube electrode
    Seiji Takeda, Shu-Ping Hui, Keisuke Fukuda, Hirotoshi Fuda, Shigeki Jin, Toshihiro Sakurai, Atsushi Ishii, Koichi Mukasa, Kazuhisa Sueoka, Hitoshi Chiba
    SENSORS AND ACTUATORS B-CHEMICAL, 166, 833, 836, May 2012, [Peer-reviewed]
    English, Scientific journal
  • Isolation and Characterization of a Phenolic Antioxidant from the Pacific Oyster (Crassostrea gigas)
    Mitsugu Watanabe, Hirotoshi Fuda, Shigeki Jin, Toshihiro Sakurai, Futaba Ohkawa, Shu-Ping Hui, Seiji Takeda, Takayuki Watanabe, Takao Koike, Hitoshi Chiba
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60, 3, 830, 835, Jan. 2012, [Peer-reviewed]
    English, Scientific journal
  • Application of Kelvin force microscopy for evaluation of oxidized low-density lipoprotein
    Seiji Takeda, Futaba Ohkawa, Toshihiro Sakurai, Shigeki Jin, Hirotoshi Fuda, Shu-Ping Hui, Hitoshi Chiba, Kazuhisa Sueoka
    2012 IEEE SENSORS PROCEEDINGS, 1751, 1754, 2012, [Peer-reviewed]
    English, International conference proceedings
  • New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase
    Takeshi Senoura, Shigeaki Ito, Hidenori Taguchi, Mariko Higa, Shigeki Hamada, Hirokazu Matsui, Tadahiro Ozawa, Shigeki Jin, Jun Watanabe, Jun Wasaki, Susumu Ito
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 408, 4, 701, 706, May 2011, [Peer-reviewed]
    English, Scientific journal
  • Rice Os4BGlu12 is a wound-induced beta-glucosidase that hydrolyzes cell wall-beta-glucan-derived oligosaccharides and glycosides
    Rodjana Opassiri, Janjira Maneesan, Takashi Akiyama, Busarakum Pomthong, Shigeki Jin, Atsuo Kimura, James R. Ketudat Cairns
    PLANT SCIENCE, 179, 3, 273, 280, Sep. 2010, [Peer-reviewed]
    English, Scientific journal
  • Measurement of lipoprotein particle sizes using dynamic light scattering
    Toshihiro Sakurai, Suchin Trirongjitmoah, Yuka Nishibata, Takeshi Namita, Masahiro Tsuji, Shu-Ping Hui, Shigeki Jin, Koichi Shimizu, Hitoshi Chiba
    ANNALS OF CLINICAL BIOCHEMISTRY, 47, 5, 476, 481, Sep. 2010, [Peer-reviewed]
    English, Scientific journal
  • Analyses for phosphatidylcholine hydroperoxides by LC/MS
    Shu-Ping Hui, Hitoshi Chiba, Shigeki Jin, Hironori Nagasaka, Takao Kurosawa
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 878, 20, 1677, 1682, Jun. 2010, [Peer-reviewed]
    English, Scientific journal
  • Expression of an endo-(1,3;1,4)-beta-glucanase in response to wounding, methyl jasmonate, abscisic acid and ethephon in rice seedlings
    Takashi Akiyama, Shigeki Jin, Midori Yoshida, Tamotsu Hoshino, Rodjana Opassiri, James R. Ketudat Cairns
    JOURNAL OF PLANT PHYSIOLOGY, 166, 16, 1814, 1825, 2009, [Peer-reviewed]
    English, Scientific journal
  • Biotransformation of (+)-catechin into taxifolin by a two-step oxidation: Primary stage of (+)-catechin metabolism by a novel (+)-catechin-degrading bacteria, Burkholderia sp KTC-1, isolated from tropical peat
    Motoki Matsuda, Yuichiro Otsuka, Shigeki Jin, Jun Wasaki, Jun Watanabe, Toshihiro Watanabe, Mitsuru Osaki
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 366, 2, 414, 419, Feb. 2008, [Peer-reviewed]
    English, Scientific journal
  • Proteomic analysis off calcium-dependent secretion in Toxoplasma gondii
    Osamu Kawase, Yoshifumi Nishikawa, Hiroshi Bannai, Houshuang Zhang, Guohong Zhang, Shigeki Jin, Eung-Goo Lee, Xuenan Xuan
    PROTEOMICS, 7, 20, 3718, 3725, Oct. 2007, [Peer-reviewed]
    English, Scientific journal
  • Molecular cloning and characterization of an arginine decarboxylase gene up-regulated by chilling stress in rice seedlings
    Takashi Akiyama, Shigeki Jin
    JOURNAL OF PLANT PHYSIOLOGY, 164, 5, 645, 654, May 2007, [Peer-reviewed]
    English, Scientific journal
  • Structural elucidation of 4-(cystein-S-yl)butyl glucosinolate from the leaves of Eruca sativa
    Sun-Ju Kim, Chiami Kawaharada, Shigeki Jin, Makoto Hashimoto, Gensho Ishii, Hiroaki Yamauchi
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 71, 1, 114, 121, Jan. 2007, [Peer-reviewed]
    English, Scientific journal
  • Structural investigation of methane hydrate sediments by microfocus X-ray computed tomography technique under high-pressure conditions
    Shigeki Jin, Jiro Nagao, Satoshi Takeya, Yusuke Jin, Junko Hayashi, Yasushi Kamata, Takao Ebinuma, Hideo Narita
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 45, 24-28, L714, L716, Jul. 2006, [Peer-reviewed]
    English, Scientific journal
  • Accumulation of 3-O-feruloylquinic acid induced by low temperature in wheat.
    Jin S, Yoshida M
    Cryobiology and Cryotechnology, 51, 57, 62, Dec. 2005, [Peer-reviewed]
    English, Scientific journal
  • Distribution of hydrate saturation ratios in artificial methane hydrate sediments measured by high-speed X-ray computerized tomography
    M Sato, S Takeya, J Nagao, S Jin, Y Kamata, H Minagawa, T Ebinuma, H Narita
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 44, 1A, 473, 475, Jan. 2005, [Peer-reviewed]
    English, Scientific journal
  • Probing fickian and non-fickian diffusion of CO2 in poly(methyl methacrylate) using in situ Raman spectroscopy and microfocus X-ray computed tomography
    JH Yoon, T Kawamura, S Takeya, SK Jin, Y Yamamoto, T Komai, M Takahashi, AV Nawaby, YP Handa
    MACROMOLECULES, 37, 25, 9302, 9304, Dec. 2004, [Peer-reviewed]
    English, Scientific journal
  • Isolation and structural elucidation of 4-(beta-D-glucopyranosyldisulfanyl)butyl glucosinolate from leaves of rocket salad (Eruca sativa L.) and its antioxidative activity
    SJ Kim, S Jin, G Ishii
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68, 12, 2444, 2450, Dec. 2004, [Peer-reviewed]
    English, Scientific journal
  • Structure analyses of artificial methane hydrate sediments by microfocus X-ray computed tomography
    S Jin, S Takeya, J Hayashi, J Nagao, Y Kamata, T Ebinuma, H Narita
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS, 43, 8A, 5673, 5675, Aug. 2004, [Peer-reviewed]
    English, Scientific journal
  • Accumulation of hydroxycinnamic acid amides in winter wheat under snow
    S Jin, M Yoshida, T Nakajima, A Murai
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 67, 6, 1245, 1249, Jun. 2003, [Peer-reviewed]
    English, Scientific journal
  • Benzoquinone, the substance essential for antibacterial activity in aqueous extracts from succulent young shoots of the pear Pyrus spp.
    S Jin, N Sato
    PHYTOCHEMISTRY, 62, 1, 101, 107, Jan. 2003, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • Antifungal compound, feruloylagmatine, induced in winter wheat exposed to a low temperature
    S Jin, M Yoshida
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 64, 8, 1614, 1617, Aug. 2000, [Peer-reviewed]
    English, Scientific journal
  • THE STRUCTURE OF A METABOLITE FROM LEAVES OF WINTER-WHEAT INOCULATED WITH H2O2
    H TAKASHIMA, S JIN, M YOSHIDA, J ABE, A MURAI
    CHEMISTRY LETTERS, 11, 1015, 1016, Nov. 1995, [Peer-reviewed]
    English, Scientific journal

Other Activities and Achievements

Research Themes

  • Development of a new method of drug toxicology for severe decomposed cases based on forensic entomotoxicology
    Grants-in-Aid for Scientific Research
    01 Apr. 2022 - 31 Mar. 2025
    的場 光太郎, 神 繁樹
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 22K10599
  • Evaluate the usefulness of postmortem CT and MRI for drug toxicology
    Grants-in-Aid for Scientific Research
    01 Apr. 2019 - 31 Mar. 2022
    Hyodoh Hideki
    Postmortem CT or MRI can be used to provide a reasonable index of suspicion that a drug or toxic substance may have caused death, but there are limitations to its high accuracy. However, the use of postmortem CT or postmortem MRI can provide a highly accurate index of suspicion that death may have been caused by drugs or toxic substances, but this method is limited in its ability to provide a highly accurate diagnosis.
    The final year's study revealed that postmortem images can be used to objectively evaluate fatty degeneration of the liver, and that they correlate well with histopathological examination results, and can determine metabolic abnormalities caused by drugs and toxic substances with a high degree of accuracy. Further research is needed.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 19K08114
  • Development and application of a new indicator for estimating postmortem interval using a chemical approach
    Grants-in-Aid for Scientific Research
    01 Apr. 2018 - 31 Mar. 2022
    Jin Shigeki
    We analyzed body fluids from cases at various postmortem intervals and found that high concentrations of n-butyric acid were detected in samples obtained from severely decomposed cases. n-Butyric acid was detected in decomposed cases at an average concentration more than 100 and 300 times higher than in non-composed cases, in blood and urine, respectively. These results suggest that the concentration of n-butyric acid in body fluids is a useful indicator of body decomposition. On the other hand, thiosulfate, a marker of hydrogen sulfide poisoning, was detected at high concentrations in severely decomposed cases that was not hydrogen sulfide poisoning, indicating the need for caution in the diagnosis of poisoning.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 18K10117
  • The development of hydrogen sulfide poisoning diagnosis method by measuring thiosulfate concentration with LC-MS/MS
    Grants-in-Aid for Scientific Research
    01 Apr. 2017 - 31 Mar. 2019
    Matoba Kotaro, Hyodoh Hideki, Jin Shigeki
    The thiosulfate concentration in blood has been recognized as a marker of hydrogen sulfide poisoning in forensic medicine. In this study, we established a new method of quantitative analysis of thiosulfate concentration using LC-MS/MS instead of GC-MS, and studied the changes in thiosulfate concentration caused by postmortem decomposition of 3 kinds of body fluids (blood, urine, and pericardial effusion) collected from forensic autopsy cases. The thiosulfate concentration in each body fluid in the decomposed cases (non-hydrogen sulfide poisoning cases) was detected at a high level, at the same level as that in hydrogen sulfide poisoning cases.Therefore, thiosulfate in body fluids is produced by postmortem decomposition regardless of hydrogen sulfide poisoning, and it is necessary to consider the influence of postmortem decomposition in the diagnosis of hydrogen sulfide poisoning based on thiosulfate concentration.
    Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, 17K15874
  • Possibility of a new biomarker in the diagnosis of primary aldosteronism
    Grants-in-Aid for Scientific Research
    01 Apr. 2013 - 31 Mar. 2017
    JIN SHIGEKI, CHIBA Hitoshi, HUI Shu-Ping, WADA Norio
    Primary aldosteronism is considered as approximately 10% of the causes of hypertension, so the diagnosis of its subtype is crucially important issue because cures are required on different kind of subtype. In this study, we found the substance which was detected more amount in the aldosterone-producing adenoma (APA) patient urine than that in the other subtypes of primary aldosteronism. We investigated the possibility of employing this substance as a new biomarker. The substance was detected as 1.5-2.0 fold amount in APA against essential hypertension and idiopathic hyperaldosteronism (IHA). However, its isolation and the structural analysis had not been executed until now.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 25460668
  • Hepatoprotective effects of novel antioxidant isolated from the Pacific oyster
    Grants-in-Aid for Scientific Research
    01 Apr. 2013 - 31 Mar. 2016
    Fuda Hirotoshi, HIRAMATSU Naoshi, JIN Shigeki
    This study was undertaken to characterize the hepatoprotective potency of 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), a novel phenolic antioxidant, isolated from the Pacific oyster, using hepatoma-derived cells (C3A) cultured in the presence of pro-oxidant, as well as well-known antioxidants. We found that only DHMBA significantly protected C3A cells against cell death in a dose-dependent manner, whereas other antioxidants did not. Also our study assessed the effects of DHMBA-rich oyster extracts (DOE) in a novel non-alcoholic steatohepatitis (NASH) model mouse established by combination of a high-fat diet and oxidized low-density lipoprotein. The NASH model mouse exhibited obesity, insulin resistance, hepatic steatosis, inflammation, fibrosis, and apoptosis. These changes were significantly moderated by supplementation of DOE. From these results, DHMBA might serve as a functional food for people at elevated risk for ROS-related diseases, such as NASH.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 25450297
  • Effective early prevention of osteoporosis caused by mutation of the gene coding for the aldehyde dehydrogenase 2 enzyme.
    Grants-in-Aid for Scientific Research
    01 Apr. 2012 - 31 Mar. 2015
    HOSHI HIROKO, JIN Shigeki
    Aldehyde dehydrogenase 2 (ALDH2) has a major role in acetaldehyde detoxification and is localized in mitochondria. About half of Japanese and East Asians have a mutant ALDH2 gene, resulting in sensitivity to alcohol. We previously showed that a dominant-negative form of Aldh2 mice showed osteoporosis caused by impaired osteoblastogenesis. In this study, we examined whether or not various nutritional factors have effects of antioxidant reagents to prevent osteoporosis in Aldh2 transgenic (ALDH2-DAL）mice and in their osteoclast cells. As the results, astaxanthin effectively inhibited the failure of osteoblasts to differentiate in a dose-dependent manner. The administration of astaxanthin to ALDH2-DAL mice led to an increasing femur bone density compared to normal-diet ALDH2-DAL mice fed for 3 months in vivo experiments. In this study, we showed that astaxanthin prevented osteoporosis caused by ALDH2 gene mutation.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), 24614014