Mar. 2024, 公益社団法人高分子学会, 令和5年度高分子科学功績賞　　　　　　　　　　　　　　　
複合糖質の高分子科学研究
西村 紳一郎

Feb. 2022, Hokkaido University, Hokkaido University President's Award for Excellence in Research and Teaching for AY2022　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura

Apr. 2020, Ministry of Education, Culture, Sports, Science and Technology-Japan, MEXT (Minister of Education, Culture, Sports, Science and Technology) Minister’s, Science and Technology for AY2020　　　　　　　　　　　　　　　
革新的創薬イノベーションを目指す糖鎖研究
Shin-Ichiro Nishimura, International academic award

Jun. 2009, Cabinet Office, 7th Industry-Academia-Government Collaboration Contribution Award　　　　　　　　　　　　　　　
大規模糖鎖解析装置及び疾患マーカー探索技術の開発
Shin-Ichiro Nishimura, International academic award, Japan

Nov. 2008, The Hokkaido Shimbun Press, The Hokkaido Shimbun Culture Prize 2008　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura, Publisher, Japan

Mar. 2006, Japan Association for Chemical Innovation, METI (Ministry of Economy, Trade and Industry) Minister’s Award at the GSC Awards for 2006　　　　　　　　　　　　　　　
Automated Glycoconjugate Synthesis by Artificial Golgi Apparatus
Shin-Ichiro Nishimura;Shionogi;Co., Ltd;TOYOBO;HITACHI, Japan

Sep. 2005, JST, NEDO, Outstanding Performance Awards of UBS Special Award Innovation Japan 2005　　　　　　　　　　　　　　　
Glycan Purification Kit S-Bio BlotGlyco
Shin-Ichiro Nishimura

May 2004, The Society of Polymer Science, Japan, The Award of the Society of Polymer Science, Japan　　　　　　　　　　　　　　　
Studies on Synthesis of Glycoconjugate
Shin-Ichiro Nishimura, International academic award

Mar. 1999, The Chemical Society of Japan, The Chemical Society of Japan Award for Creative Work for 1998　　　　　　　　　　　　　　　
Development of Efficient Synthesis of Glycoconjugate and Application to Its Functional Analysis
Shin-Ichiro Nishimura, International academic award

Jul. 1998, The Japanese Society of Carbohydrate Research, JSCR (The Japanese Society of Carbohydrate Research) Incentive Awards　　　　　　　　　　　　　　　
Functional Analysis of Glycoconjugate and Its Application
Shin-Ichiro Nishimura

May 1997, Johns Hopkins University, Membership of the Johns Hopkins Society of Scholars　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
United States

Brain lipidomics for region-specific biomarker discovery in neurodegenerative diseases
Jayashankar Jayaprakash, Solomon Tebeje Gizaw, Divyavani Gowda, Hiroshi Hinou, Shin-Ichiro Nishimura, Shu-Ping Hui, Siddabasave Gowda B. Gowda
FRONTIERS IN AGING NEUROSCIENCE, 18, 11 Feb. 2026
English, Scientific journal

Nanosomal Irinotecan Targeting Pancreatic Cancer Cell Surface Neuraminidase‐1 Sialidase
Ken Murakami, Yasuhiro Yokoi, Nozomi Hirane, Michiru Otaki, Shin‐Ichiro Nishimura
Advanced Healthcare Materials, Wiley, 22 Jul. 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Abstract

Pancreatic ductal adenocarcinoma (PDAC) is an increasing incidence and is the deadliest cancer. Most pancreatic cancer patients present with nonspecific symptoms at an advanced disease stage that is not amenable to curative surgery. Therapies by immune checkpoint inhibitors have proven difficult in the patients with PDAC. It is communicated that cell surface neuraminidase‐1 (Neu‐1) is a highly potential target molecule for nanomedicine to improve the therapeutic efficacy and safety of the present first‐line therapies in patients with advanced PDAC. Nanosomes composed of an inorganic core and antiadhesive shell of phospholipid monolayer enable active delivery of irinotecan into intracellular space by targeting Neu‐1. Nanosomal irinotecan is endocytosed efficiently and exhibits synergistically enhanced inhibitory effects on the growth of Panc‐1 cells in vitro (IC₅₀ = 8.07 nm) when displayed with an irreversible‐mechanism based Neu‐1 inhibitor that inactivates nucleophiles of Neu‐1 by a covalent bond formation. Strikingly, in vivo antitumor effects of nanosomal irinotecan revealed that tumor growth in Panc‐1 xenograft model is inhibited completely by injection at a dose of 5 mg kg⁻¹ six times at 4‐day intervals without body weight loss. The preliminary results provide evidence that targeting Neu‐1 by nanomedicine is a promising drug delivery strategy to a desired chemotherapy in the patients with advanced PDAC.

Site-Specific O-Glycosylation in Oncofetal Fibronectin IIICS Domain Creates Cancer Stage-Specific Biomarkers.
Nozomi Hirane, Yasuhiro Yokoi, Aiko Kobayashi, Misaki Yamada, Taiki Konno, Hiroyuki Kumeta, Hajime Wakui, Michiru Otaki, Yoshiaki Miura, Tatsuhiko Kakisaka, Toshiya Kamiyama, Akinobu Taketomi, Kazuhiro Nouso, Satoshi Kimura, Shin-Ichiro Nishimura
Journal of the American Chemical Society, 147, 14, 12170, 12184, 09 Apr. 2025, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Despite the pathological importance of oncofetal fibronectin isoforms associated with cancer cell progression and metastasis, our understanding of the structural and molecular details that occurred in this abundant and ubiquitous extracellular matrix component remains largely unknown. Here, we communicate that site-specific O-glycosylation in the oncofetal fibronectin creates cancer stage-specific serum biomarkers for hepatocellular carcinoma (HCC) by quantitative MS-based glycoproteomic approach. Selective reaction monitoring (SRM) using a structure-defined synthetic glycopeptide library enabled absolute quantitation of the targeted label-free tryptic fragments bearing cancer-relevant O-glycans derived from the type III homology connective segment (IIICS) domain of the oncofetal fibronectin (onfFN) in the sera of HCC patients and healthy controls. We found site-specific O-glycoform alteration from T/sialyl T antigens to Tn/sialyl Tn antigens at the consecutive threonine residues 2155Thr-Thr-Ala2157 within this fragment during cancer progression. Surprisingly, this dynamic glycoform alteration is observed specifically in the patient sera diagnosed newly as stage 2-4 groups not in the normal control and stage 1 patient groups. Our results provide compelling evidence that site-specific glycoform changes observed in the onfFN IIICS domain during the tumor proliferation elaborate new class cancer stage-relevant "dynamic epitopes" as highly potential cancer diagnostic and therapeutic targets.

Smart Nanomedicine Targeting Endocytosis Mediated by Cancer Cell Surface Neuraminidase‐1
Ken Murakami, Daiki Kambe, Yasuhiro Yokoi, Hajime Wakui, Shun Hayakawa, Nozomi Hirane, Ryosuke Koide, Michiru Otaki, Noriko Nagahori, Shin-Ichiro Nishimura
Advanced NanoBiomed Research, 3, 12, Wiley, 08 Nov. 2023, [Peer-reviewed], [Corresponding author]
Scientific journal, Human neuraminidase‐1 (NEU1) plays a much more profound function in human cancers than previously considered. It is demonstrated that cancer cell surface NEU1 is a desired gatekeeper for an innovative anticancer therapeutic nanomedicine enabling active drug‐targeting delivery by specific endocytosis into the cytoplasm. Nanosome, an antiadhesive nanoparticular shuttle, carrying multiple suicide substrates for NEU1 confers potent and universal inhibitory effects on the proliferation of human cancer cells, such as hepatocellular carcinoma (HCC) (HepG2, IC₅₀ = 13.5 nM), lung cancer (A549, IC₅₀ = 9.57 nM), and colon cancer (HT‐29, IC₅₀ = 11.1 nM), in which irreversible inactivation of cell surface NEU1 is essential for the intracellular trafficking and subsequent lysosomal membrane permeabilization by nanosomal aggregation due to the formation of “sialidase corona” through irreversible inactivation of NEU1–NEU4 residing in lysosome. Nanomedicine targeting membrane‐tethered NEU1 allows efficient delivery of hydrophobic sorafenib (Nexavar), a RAF family kinase inhibitor for the treatment of advanced renal cell carcinoma and unresectable HCC at the recommended dose of 400 mg orally twice daily, into endolysosome, resulting in a potent and sustainable inhibition (IC₅₀ = 3.1–6.2 nM at 24–96 h after coincubation) against HepG2 cell growth.

Altering the Modular Architecture of Galectins Affects its Binding with Synthetic α‐Dystroglycan O‐Mannosylated Core M1 Glycoconjugates In situ
Lareno L. Villones, Anna-Kristin Ludwig, Seiya Kikuchi, Rika Ochi, Shin-Ichiro Nishimura, Hans-Joachim Gabius, Herbert Kaltner, Hiroshi Hinou
ChemBioChem, Wiley, 09 Mar. 2023, [Peer-reviewed]
English, Scientific journal

Structural and molecular insight into antibody recognition of dynamic neoepitopes in membrane tethered MUC1 of pancreatic cancer cells and secreted exosomes
Hajime Wakui, Yasuhiro Yokoi, Chieko Horidome, Toyoyuki Ose, Min Yao, Yoshikazu Tanaka, Hiroshi Hinou, Shin-Ichiro Nishimura
RSC Chemical Biology, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed], [Corresponding author]
English, Scientific journal, We unveil for the first time that pancreatic cancer cells (PANC-1) and secreted exosomes express MUC1 bearing cancer-relevant dynamic epitopes recognized specifically by an anti-MUC1 antibody (SN-131).

Mouse tissue glycome atlas 2022 highlights inter-organ variation in major N-glycan profiles
Michiru Otaki, Nozomi Hirane, Yayoi Natsume-Kitatani, Mari Nogami Itoh, Masanori Shindo, Yoichi Kurebayashi, Shin-Ichiro Nishimura
Scientific Reports, 12, 1, 17804, Springer Science and Business Media LLC, 24 Oct. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, Abstract

This study presents “mouse tissue glycome atlas” representing the profiles of major N-glycans of mouse glycoproteins that may define their essential functions in the surface glycocalyx of mouse organs/tissues and serum-derived extracellular vesicles (exosomes). Cell surface glycocalyx composed of a variety of N-glycans attached covalently to the membrane proteins, notably characteristic “N-glycosylation patterns” of the glycocalyx, plays a critical role for the regulation of cell differentiation, cell adhesion, homeostatic immune response, and biodistribution of secreted exosomes. Given that the integrity of cell surface glycocalyx correlates significantly with maintenance of the cellular morphology and homeostatic immune functions, dynamic alterations of N-glycosylation patterns in the normal glycocalyx caused by cellular abnormalities may serve as highly sensitive and promising biomarkers. Although it is believed that inter-organs variations in N-glycosylation patterns exist, information of the glycan diversity in mouse organs/tissues remains to be elusive. Here we communicate for the first-time N-glycosylation patterns of 16 mouse organs/tissues, serum, and serum-derived exosomes of Slc:ddY mice using an established solid-phase glycoblotting platform for the rapid, easy, and high throughput MALDI-TOFMS-based quantitative glycomics. The present results elicited occurrence of the organ/tissue-characteristic N-glycosylation patterns that can be discriminated to each other. Basic machine learning analysis using this N-glycome dataset enabled classification between 16 mouse organs/tissues with the highest F1 score (69.7–100%) when neural network algorithm was used. A preliminary examination demonstrated that machine learning analysis of mouse lung N-glycome dataset by random forest algorithm allows for the discrimination of lungs among the different mouse strains such as the outbred mouse Slc:ddY, inbred mouse DBA/2Crslc, and systemic lupus erythematosus model mouse MRL-lpr/lpr with the highest F1 score (74.5–83.8%). Our results strongly implicate importance of “human organ/tissue glycome atlas” for understanding the crucial and diversified roles of glycocalyx determined by the organ/tissue-characteristic N-glycosylation patterns and the discovery research for N-glycome-based disease-specific biomarkers and therapeutic targets.

Exploring the In situ pairing of human galectins toward synthetic O-mannosylated core M1 glycopeptides of α-dystroglycan
Lareno L. Villones, Anna-Kristin Ludwig, Hiroyuki Kumeta, Seiya Kikuchi, Rika Ochi, Tomoyasu Aizawa, Shin-Ichiro Nishimura, Hans-Joachim Gabius, Hiroshi Hinou
Scientific Reports, 12, 1, 17800, Springer Science and Business Media LLC, 23 Oct. 2022, [Peer-reviewed]
English, Scientific journal, Abstract

Dystroglycan (DG), which constitutes a part of the dystrophin–glycoprotein complex, connects the extracellular matrix to the cytoskeleton. The matriglycans presented by the extracellular α-DG serve as a contact point with extracellular matrix proteins (ECM) containing laminin G-like domains, providing cellular stability. However, it remains unknown whether core M1 (GlcNAcβ1-2Man) structures can serve as ligands among the various O-Mannosylated glycans. Therefore, based on the presence of N-acetylLactosamine (LacNAc) in this glycan following the core extension, the binding interactions with adhesion/growth-regulatory galectins were explored. To elucidate this process, the interaction between galectin (Gal)-1, -3, -4 and -9 with α-DG fragment ³⁷²TRGAIIQTPTLGPIQPTRV³⁹⁰ core M1-based glycopeptide library were profiled, using glycan microarray and nuclear magnetic resonance studies. The binding of galectins was revealed irrespective of its modular architecture, adding galectins to the list of possible binding partners of α-DG core M1 glycoconjugates by cis-binding (via peptide- and carbohydrate-protein interactions), which can be abrogated by α2,3-sialylation of the LacNAc units. The LacNAc-terminated α-DG glycopeptide interact simultaneously with both the S- and F-faces of Gal-1, thereby inducing oligomerization. Furthermore, Gal-1 can trans-bridge α-DG core M1 structures and laminins, which proposed a possible mechanism by which Gal-1 ameliorates muscular dystrophies; however, this proposal warrants further investigation.

Generation 6 polyamidoamine dendrimer provides an ideal nanoparticular platform for enzyme-assisted synthesis of glycopeptides having bulky and complex glycans
Takahiko Matsushita, Hiroshi Hinou, Shin-Ichiro Nishimura
Chemistry Letters, 51, 1044, 1048, The Chemical Society of Japan, 16 Sep. 2022, [Peer-reviewed]
English, Scientific journal

Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
Kouta Shiratori, Yasuhiro Yokoi, Hajime Wakui, Nozomi Hirane, Michiru Otaki, Hiroshi Hinou, Tohru Yoneyama, Shingo Hatakeyama, Satoshi Kimura, Chikara Ohyama, Shin-Ichiro Nishimura
RSC advances, 12, 33, 21385, 21393, 21 Jul. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases. The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC. To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers. Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls. Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls. The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.

Clinical utility of a serum glycome analysis in patients with colorectal cancer
Takei D, Harada K, Nouso K, MiyaharaK, Dohi C, Matsushita H, Kinugasa H, Hiraoka S, Nishimura S-I, Okada H
J. Gastroen. Hepatol., 37, 4, 727, 733, 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND AND AIM: Serum glycans are known to be good markers for the early diagnosis and prognostic prediction in many cancers. The aims of this study were to reveal the serum glycan changes comprehensively during the process of carcinogenesis from colorectal adenoma (CRA) to colorectal cancer (CRC) and to evaluate the usefulness of the glycan profiles as clinical markers for CRC. METHODS: Serum samples were obtained from 80 histologically proven CRC and 36 CRA cases. The levels of glycans in the serum were examined with a comprehensive, quantitative, high-throughput unique glycome analysis, and their diagnostic and prognostic abilities were evaluated. RESULTS: Among 34 stably detected glycans, nine were differentially expressed between CRC and CRA. Serum levels of hybrid type glycans were increased in patients with CRC compared with those with CRA (P < 0.001), and both hybrid-type and multi-antennary glycans were significantly increased in advanced cancer cases. The glycan, m/z 1914, showed the highest diagnostic value among the decreased glycans, whereas m/z 1708 showed the highest among the increased glycans. The glycan ratio m/z 1708/1914 showed a higher area under the receiver operating characteristic curve (0.889) than any other single glycan or conventional tumor marker, such as carcinoembryonic antigen (0.766, P = 0.040) and carbohydrate antigen 19-9 (0.615, P < 0.001). High m/z 1708/1914 was also correlated with an advanced cancer stage and short overall survival. CONCLUSION: Serum glycans, especially the m/z 1708/1914 ratio, were useful for the diagnosis, staging, and prognosis prediction of CRC.

Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis
Koidea R, Hirane N, Kambea D, Yoko Y, Otakia M, Nishimura S-I
Biomaterials, 280, 121314, Jan. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Erratum to “Cluster binding studies with two anti-Thomsen-Friedenreich (anti-core-1, CD176) antibodies: Evidence for a quadruple epitope” [Int. Immunopharmacol. 72 (2019) 186–194] (International Immunopharmacology (2019) 72 (186–194), (S1567576918308750), (10.1016/j.intimp.2019.03.058))
A. Flechner, G. Butschak, A. Löffler, J. Rühmann, S. I. Nishimura, R. Dölling, B. Purfürst, S. Goletz, A. Danielczyk, U. Karsten
International Immunopharmacology, 93, Elsevier B.V., 01 Apr. 2021
English, Scientific journal

A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes (vol 11, pg 4999, 2020)
Hajime Wakui, Yoshikazu Tanaka, Toyoyuki Ose, Isamu Matsumoto, Koji Kato, Yao Min, Taro Tachibana, Masaharu Sato, Kentaro Naruchi, Fayna Garcia Martin, Hiroshi Hinou, Shin-Ichiro Nishimura
CHEMICAL SCIENCE, 11, 46, 12588, 12589, Dec. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines
Hidenori Takahashi, Toshiya Kamiyama, Nozomi Hirane, Nozomi Kobayashi, Takeshi Aiyama, Akihisa Nagatsu, Shingo Shimada, Tatsuya Orimo, Tatsuhiko Kakisaka, Moto Fukai, Hideki Yokoo, Hirofumi Kamachi, Shin‑Ichiro Nishimura, Akinobu Taketomi
Oncology Reports, 44, 6, 2757, 2769, Spandidos Publications, 08 Oct. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, The N‑glycoforms of glycoproteins modify protein function and control a number of biological pathways. The aim of the present study was to investigate the correlation between alterations in N‑glycans and cancer aggressiveness in terms of cancer cell invasion ability. The expression of urokinase‑type plasminogen activator (uPA) and N‑acetylglucosaminyltransferase V (GnT‑V) in liver cancer cell lines was analyzed by western blotting. Cell invasiveness was analyzed by Matrigel invasion assays. uPA and GnT‑V expression in liver cancer cell lines was knocked down by RNA interference. Furthermore, uPA was overexpressed in liver cancer cells using lentiviral vectors, and a mutant strain of HepG2 cells overexpressing uPA deficient in N‑glycans was established. A glycoblotting‑assisted matrix‑assisted laser desorption/ionization‑time‑of‑flight/mass spectrometry‑based quantitative analysis of liver cancer cell lines was performed, in which invasiveness was altered by modifying the expression of uPA and GnT‑V. N‑glycan profiles were found to differ between the highly invasive liver cancer cell line HLE and the less invasive cell line HepG2. The expression of several N‑glycans, including a form with m/z=1892, was changed according to invasiveness controlled by knockdown and overexpression of uPA. The invasiveness of HepG2 cells with mutant uPA did not increase regardless of the level of expression of uPA. Following GnT‑V knockdown and N‑glycan alteration, uPA expression did not change, whereas cell invasiveness decreased. One N‑glycan (m/z=1892) was common among N‑glycans in the comparative analysis between HLE and HepG2, HLE and uPA knockdown HLE, HepG2 and uPA‑overexpressing HepG2, and HLE and GnT‑V knockdown HLE cells and among N‑glycan profiles in human uPA. Therefore, N‑glycosylation is an important factor controlling invasiveness of liver cancer cells, and a specific N‑glycan (m/z=1892) associated with the invasion of liver cancer cells via uPA was identified in the present study.

Effect of Site‐Specific O ‐Glycosylation on the Structural Behavior of NOTCH1 Receptor Extracellular EGF‐like Domains 11 and 10
Yasuhiro Yokoi, Shin‐Ichiro Nishimura
Chemistry – A European Journal, 26, 54, 12363, 12372, Wiley, 25 Sep. 2020, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Amplified Detection of Breast Cancer Autoantibodies Using MUC1-Based Tn Antigen Mimics
Pablo A. Guillen-Poza, Elena M. Sánchez-Fernández, Gerard Artigas, José Manuel García Fernández, Hiroshi Hinou, Carmen Ortiz Mellet, Shin-Ichiro Nishimura, Fayna Garcia-Martin
Journal of Medicinal Chemistry, 63, 15, 8524, 8533, American Chemical Society (ACS), 13 Aug. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, In many human carcinomas, mucin-1 (MUC1) is overexpressed and aberrantly glycosylated, resulting in the exposure of previously hidden antigens. This generates new patient antibody profiles that can be used in cancer diagnosis. In the present study, we focused on the MUC1-associated Tn antigen (α-O-GalNAc-Ser/Thr) and substituted the GalNAc monosaccharide by a glycomimic to identify MUC1-based glycopeptides with increased antigenicity. Two different glycopeptide libraries presenting the natural Tn antigen or the sp2-iminosugar analogue were synthesized and evaluated with anti-MUC1 monoclonal antibodies in a microarray platform. The most promising candidates were tested with healthy and breast cancer sera aiming for potential autoantibody-based biomarkers. The suitability of sp2-iminosugar glycopeptides to detect anti-MUC1 antibodies was demonstrated, and serological experiments showed stage I breast cancer autoantibodies binding with a specific unnatural glycopeptide with almost no healthy serum interaction. These results will promote further studies on their capabilities as early cancer biomarkers.

Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.
Shun Hayakawa, Takahiko Matsushita, Yasuhiro Yokoi, Hajime Wakui, Fayna Garcia-Martin, Hiroshi Hinou, Koji Matsuoka, Kazuhiro Nouso, Toshiya Kamiyama, Akinobu Taketomi, Shin-Ichiro Nishimura
Biochemistry, 59, 12, 1221, 1241, 31 Mar. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy. This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs). NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs. We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region. Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension. An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC). Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients. Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.

Serum N-glycan profiling can predict biopsy-proven graft rejection after living kidney transplantation.
Osamu Soma, Shingo Hatakeyama, Tohru Yoneyama, Mitsuru Saito, Hideo Sasaki, Yuki Tobisawa, Daisuke Noro, Yuichiro Suzuki, Masakazu Tanaka, Shin-Ichiro Nishimura, Hiroshi Harada, Hideki Ishida, Kazunari Tanabe, Shigeru Satoh, Chikara Ohyama
Clinical and experimental nephrology, 24, 2, 174, 184, Feb. 2020, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, BACKGROUND: To evaluate whether serum N-glycan profile can be used as a diagnostic marker of graft rejection after living-donor kidney transplants (KT). METHODS: We retrospectively examined 174 KT recipients at five medical centers. N-Glycan levels were analyzed in postoperative serum samples using glycoblotting combined with mass spectrometry. We developed an integrated score to predict graft rejection based on a combination of age, gender, immunological risk factors, and serum N-glycan levels at post-KT day D1 and D7. Rejection-free survival rates stratified by the sum of integrated scores (D1 + D7) were evaluated using Kaplan-Meier curves. RESULTS: Of 174, 52 showed graft rejection (Rejection-pos. group) and 122 recipients did not show graft rejection (Rejection-neg. group). The integrated scores were significantly higher in the Rejection-pos. group than those of the Rejection-neg. group. Area-under-curve (AUC) value of integrated scores at post-KT D1, and D7 were 0.84 and 0.84, respectively. The sum of integrated scores (D1 + D7) ≥ 0.50 identified graft rejection with 81% sensitivity and 80% specificity; with an AUC value of 0.87. Recipients with higher sum of integrated scores (D1 + D7 ≥ 0.5) had significantly shorter rejection-free survival than those with lower scores. CONCLUSION: Evaluation of serum N-glycosylation profiles can identify recipients who are prone to rejection.

Synthesis, conformational analysis and in vivo assays of an anti-cancer vaccine that features an unnatural antigen based on an sp2-iminosugar fragment
Iris A. Bermejo, Claudio D. Navo, Jorge Castro-López, Ana Guerreiro, Ester Jiménez-Moreno, Elena M. Sánchez Fernández, Fayna García-Martín, Hiroshi Hinou, Shin-Ichiro Nishimura, José M. García Fernández, Carmen Ortiz Mellet, Alberto Avenoza, Jesús H. Busto, Gonçalo J. L. Bernardes, Ramón Hurtado-Guerrero, Jesús M. Peregrina, Francisco Corzana
Chemical Science, 11, 15, 3996, 4006, Royal Society of Chemistry (RSC), 2020, [Peer-reviewed]
English, Scientific journal,

An anti-cancer vaccine based on an unnatural antigen with an sp²-iminosugar fragment.

Serum N-glycan profiling is a potential biomarker for castration-resistant prostate cancer
Teppei Matsumoto, Shingo Hatakeyama, Tohru Yoneyama, Yuki Tobisawa, Yusuke Ishibashi, Hayato Yamamoto, Takahiro Yoneyama, Yasuhiro Hashimoto, Hiroyuki Ito, Shin-Ichiro Nishimura, Chikara Ohyama
Scientific Reports, 9, 1, Springer Science and Business Media LLC, Dec. 2019, [Peer-reviewed]
English, Scientific journal

Antiadhesive Nanosomes Facilitate Targeting of the Lysosomal GlcNAc Salvage Pathway through Derailed Cancer Endocytosis
Ryosuke Koide, Shin‐Ichiro Nishimura
Angewandte Chemie International Edition, 58, 41, 14513, 14518, Wiley, 07 Oct. 2019, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles.
Sho Hideshima, Hiroki Hayashi, Hiroshi Hinou, Shunsuke Nambuya, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Shin-Ichiro Nishimura, Yoshihiro Sakoda, Tetsuya Osaka
Scientific reports, 9, 1, 11616, 11616, 12 Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Pandemic influenza, triggered by the mutation of a highly pathogenic avian influenza virus (IFV), has caused considerable damage to public health. In order to identify such pandemic IFVs, antibodies that specifically recognize viral surface proteins have been widely used. However, since the analysis of a newly discovered virus is time consuming, this delays the availability of suitable detection antibodies, making this approach unsuitable for the early identification of pandemic IFVs. Here we propose a label-free semiconductor-based biosensor functionalized with sialic-acid-containing glycans for the rapid identification of the pandemic IFVs present in biological fluids. Specific glycans are able to recognize wild-type human and avian IFVs, suggesting that they are useful in discovering pandemic IFVs at the early stages of an outbreak. We successfully demonstrated that a dual-channel integrated FET biosensing system, which were modified with 6'-sialyllactose and 3'-sialyllactose for each gate area, can directly and specifically detect human H1N1 and avian H5N1 IFV particles, respectively, present in nasal mucus. Furthermore, to examine the possibility of identifying pandemic IFVs, the signal attributed to the detection of Newcastle disease virus (NDV) particles, which was selected as a prime model of a pandemic IFV, was clearly observed from both sensing gates. Our findings suggest that the proposed glycan-immobilized sensing system could be useful in identifying new pandemic IFVs at the source of an outbreak.

Cluster binding studies with two anti-Thomsen-Friedenreich (anti-core-1, CD176, TF) antibodies: Evidence for a multiple TF epitope
A. Flechner, G. Butschak, A. Löffler, J. Rühmann, S.-I. Nishimura, R. Dölling, B. Purfürst, S. Goletz, A. Danielczyk, U. Karsten
International Immunopharmacology, 72, 186, 194, Elsevier BV, Jul. 2019, [Peer-reviewed]
English, Scientific journal

Synthetic glycopeptides reveal specific binding pattern and conformational change at O-mannosylated position of alpha-dystroglycan by POMGnT1 catalyzed GlcNAc modification
Hinou Hiroshi, Kikuchi Seiya, Ochi Rika, Igarashi Kota, Takada Wataru, Nishimura Shin-Ichiro
BIOORGANIC & MEDICINAL CHEMISTRY, 27, 13, 2822, 2831, 01 Jul. 2019, [Peer-reviewed], [Last author, Corresponding author]

Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.
Gebrehiwot AG, Melka DS, Kassaye YM, Gemechu T, Lako W, Hinou H, Nishimura SI
BMC cancer, 19, 1, 588, Jun. 2019, [Peer-reviewed], [Last author, Corresponding author]

Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species.
Sanes JT, Hinou H, Lee YC, Nishimura SI
Journal of agricultural and food chemistry, 67, 1, 531, 540, Jan. 2019, [Peer-reviewed]
English, Scientific journal

Synthetic Glycopeptides Allow for the Quantitation of Scarce Nonfucosylated IgG Fc N-Glycans of Therapeutic Antibody.
Hammura K, Ishikawa A, H V RK, Miyoshi R, Yokoi Y, Tanaka M, Hinou H, Nishimura SI
ACS medicinal chemistry letters, 9, 9, 889, 894, Sep. 2018, [Peer-reviewed]

Synthetic glycopeptides as a designated standard in focused glycoproteomics to discover serum cancer biomarkers.
Yogesh KV, Kamiyama T, Ohyama C, Yoneyama T, Nouso K, Kimura S, Hinou H, Nishimura SI
MedChemComm, 9, 8, 1351, 1358, Aug. 2018, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

網羅的糖鎖解析による肝細胞癌の浸潤能と糖鎖異常の検討　　　　　　　　　　　　　　　
高橋 秀徳, 神山 俊哉, 柿坂 達彦, 相山 健, 若山 顕治, 折茂 達也, 蒲池 浩文, 横尾 英樹, 西村 紳一郎, 武冨 紹信
日本消化器外科学会総会, 73回, 685, 685, (一社)日本消化器外科学会, Jul. 2018
Japanese

New biomarker for castration-resistant prostate cancer: A glycobiological perspective
Shingo Hatakeyama, Tohru Yoneyama, Hayato Yamamoto, Yuki Tobisawa, Shin-Ichiro Nishimura, Chikara Ohyama
Hormone Therapy and Castration Resistance of Prostate Cancer, 307, 315, Springer Singapore, 11 May 2018
English, In book

CLEC10A Is a specific marker for human CD1c+ dendritic cells and enhances their toll-like receptor 7/8-Induced cytokine secretion
Lukas Heger, Silke Balk, Jennifer J. Lühr, Gordon F. Heidkamp, Christian H.K. Lehmann, Lukas Hatscher, Ariawan Purbojo, Arndt Hartmann, Fayna Garcia-Martin, Shin-Ichiro Nishimura, Robert Cesnjevar, Falk Nimmerjahn, Diana Dudziak
Frontiers in Immunology, 9, 744, Frontiers Media S.A., 27 Apr. 2018, [Peer-reviewed]
English, Scientific journal

Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.
Gebrehiwot AG, Melka DS, Kassaye YM, Rehan IF, Rangappa S, Hinou H, Kamiyama T, Nishimura SI
PloS one, 13, 12, e0209515, 2018, [Peer-reviewed], [Last author, Corresponding author]

Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.
Ishihara S, Aoki K, Mizutani T, Amano M, Nishimura SI, Haga H
Cell structure and function, 43, 2, 177, 185, 2018, [Peer-reviewed]

Aberrant N-Glycosylation Profile of Serum Immunoglobulins is a Diagnostic Biomarker of Urothelial Carcinomas.
Toshikazu Tanaka, Tohru Yoneyama, Daisuke Noro, Kengo Imanishi, Yuta Kojima, Shingo Hatakeyama, Yuki Tobisawa, Kazuyuki Mori, Hayato Yamamoto, Atsushi Imai, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Masakazu Tanaka, Shin-Ichiro Nishimura, Shizuka Kurauchi, Ippei Takahashi, Chikara Ohyama
International journal of molecular sciences, 18, 12, 06 Dec. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal, The aim of this study to determine whether the aberrant N-glycosylated serum immunoglobulins (Igs) can be applied as a diagnostic marker of urothelial carcinoma (UC). Between 2009 and 2016, we randomly obtained serum available from 237 UC and also 96 prostate cancer as other cancer controls from our serum bank and also obtained-from 339 healthy volunteers (HV)-controls obtained from community-dwelling volunteers in Iwaki Health Promotion Project. A total of 32 types of N-glycan levels on Igs were determined by high-throughput N-glycomics and analyzed by multivariable discriminant analysis. We found five UC-associated aberrant N-glycans changes on Igs and also found that asialo-bisecting GlcNAc type N-glycan on Igs were significantly accumulated in UC patients. The diagnostic N-glycan Score (dNGScore) established by combination of five N-glycans on Igs discriminated UC patients from HV and prostate cancer (PC) patients with 92.8% sensitivity and 97.2% specificity. The area under the curve (AUC) for of the dNGScore was 0.969 for UC detection that was much superior to that of urine cytology (AUC, 0.707) and hematuria (AUC, 0.892). Furthermore, dNGScore can detect hematuria and urine cytology negative patients. The dNGscore based on aberrant N-glycosylation signatures of Igs were found to be promising diagnostic biomarkers of UCs.

The Use of Fluoroproline in MUC1 Antigen Enables Efficient Detection of Antibodies in Patients with Prostate Cancer
Victor J. Somovilla, Iris A. Bermejo, Ines S. Albuquerque, Nuria Martinez-Saez, Jorge Castro-Lopez, Fayna Garcia-Martin, Ismael Companon, Hiroshi Hinou, Shin-Ichiro Nishimura, Jesus Jimenez-Barbero, Juan L. Asensio, Alberto Avenoza, Jesus H. Busto, Ramon Hurtado-Guerrero, Jesus M. Peregrina, Goncalo J. L. Bernardes, Francisco Corzana
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 139, 50, 18255, 18261, Dec. 2017, [Peer-reviewed]
English, Scientific journal

Glycopeptides as Targets for Dendritic Cells: Exploring MUC1 Glycopeptides Binding Profile toward Macrophage Galactose-Type Lectin (MGL) Orthologs
Gerard Artigas, Joao T. Monteiro, Hiroshi Hinou, Shin-Ichiro Nishimura, Bernd Lepenies, Fayna Garcia-Martin
JOURNAL OF MEDICINAL CHEMISTRY, 60, 21, 9012, 9021, Nov. 2017, [Peer-reviewed]
English, Scientific journal

An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein
Takayuki Furukawa, Hiroshi Hinou, Seiji Takeda, Hitoshi Chiba, Shin-Ichiro Nishimura, Shu-Ping Hui
CHEMBIOCHEM, 18, 19, 1903, 1909, Oct. 2017, [Peer-reviewed]
English, Scientific journal

Chemical Synthesis Demonstrates That Dynamic O-Glycosylation Regulates the Folding and Functional Conformation of a Pivotal EGF12 Domain of the Human NOTCH1 Receptor
Shun Hayakawa, Yasuhiro Yokoi, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 56, 33, 4379, 4391, Aug. 2017, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Serum Aberrant N-Glycan Profile as a Marker Associated with Early Antibody-Mediated Rejection in Patients Receiving a Living Donor Kidney Transplant
Daisuke Noro, Tohru Yoneyama, Shingo Hatakeyama, Yuki Tobisawa, Kazuyuki Mori, Yasuhiro Hashimoto, Takuya Koie, Masakazu Tanaka, Shin-Ichiro Nishimura, Hideo Sasaki, Mitsuru Saito, Hiroshi Harada, Tatsuya Chikaraishi, Hideki Ishida, Kazunari Tanabe, Shigeru Satoh, Chikara Ohyama
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18, 8, Aug. 2017, [Peer-reviewed]
English, Scientific journal

Comparative Glycomics of Fat Globule Membrane Glycoconjugates from Buffalo (Bubalus bubalis) Milk and Colostrum
Nagaraju Brijesha, Shin-Ichiro Nishimura, Huligerepura Sosalegowda Aparna
Journal of Agricultural and Food Chemistry, 65, 8, 1496, 1506, American Chemical Society, 01 Mar. 2017, [Peer-reviewed]
English, Scientific journal

Generation of Novel Anti-MUC1 Monoclonal Antibodies with Designed Carbohydrate Specificities Using MUC1 Glycopeptide Library
Shoichi Naito, Tatsuya Takahashi, Junji Onoda, Shoko Uemura, Naoki Ohyabu, Hiroshi Takemoto, Shoji Yamane, Ikuo Fujii, Shin-Ichiro Nishimura, Yoshito Numata
ACS Omega, 2, 11, 7493, 7505, American Chemical Society, 2017, [Peer-reviewed]
English, Scientific journal

Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays
Gerard Artigas, Hiroshi Hinou, Fayna Garcia-Martin, Hans-Joachim Gabius, Shin-Ichiro Nishimura
CHEMISTRY-AN ASIAN JOURNAL, 12, 1, 159, 167, Jan. 2017, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycoblotting method allows for rapid and efficient glycome profiling of human Alzheimer's disease brain, serum and cerebrospinal fluid towards potential biomarker discovery
Solomon T. Gizaw, Tetsu Ohashi, Masakazu Tanaka, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1860, 8, 1716, 1727, Aug. 2016, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

N-Glycomic and Microscopic Subcellular Localization Analyses of NPP1, 2 and 6 Strongly Indicate that trans-Golgi Compartments Participate in the Golgi to Plastid Traffic of Nucleotide Pyrophosphatase/Phosphodiesterases in Rice
Kaneko Kentaro, Takamatsu Takeshi, Inomata Takuya, Oikawa Kazusato, Itoh Kimiko, Hirose Kazuko, Amano Maho, Nishimura Shin-Ichiro, Toyooka Kiminori, Matsuoka Ken, Pozueta-Romero Javier, Mitsui Toshiaki
PLANT AND CELL PHYSIOLOGY, 57, 8, 1610, 1628, Aug. 2016, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Inhibitory potential of Buffalo (Bubalus bubalis) colostrum immunoglobulin G on Klebsiella pneumoniae
Mamatha Bhanu L.S., S.-I. Nishimura, Aparna H.S.
International Journal of Biological Macromolecules, 88, 138, 145, Elsevier BV, Jul. 2016, [Peer-reviewed]
English, Scientific journal

Convergent Solid-Phase Synthesis of Macromolecular MUC1 Models Truly Mimicking Serum Glycoprotein Biomarkers of Interstitial Lung Diseases
Naoki Ohyabu, Kiyoshi Kakiya, Yasuhiro Yokoi, Hiroshi Hinou, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138, 27, 8392, 8395, Jul. 2016, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Short Communication: Increase of Sialylated N-Glycansin Eyes with Neovascular Glaucoma Secondary to Proliferative Diabetic Retinopathy.
Inafuku S, Noda K, Amano M, Nishimura S, Ishida S
Current eye research, 41, 5, 721, 724, May 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, PURPOSE: To investigate the alteration of N-glycans in the vitreous fluid of patients with neovascular glaucoma (NVG) secondary to proliferative diabetic retinopathy (PDR). METHODS: Vitreous samples were collected from 18 patients with PDR (including 7 with NVG and 11 without NVG), and 17 patients without diabetes. Profiles of N-glycans were analyzed by glycoblotting-based high throughput protocol, which we recently developed. Protein levels of vascular endothelial growth factor (VEGF)-A were measured by ELISA. RESULTS: The concentration of total N-glycans and the concentration of N-glycans with sialic acids were significantly higher in NVG group compared with those in non-NVG group or control group, whereas there was no significant difference in concentrations of high-mannose N-glycans among three groups. There was a moderate correlation between the concentrations of sialylated N-glycans and VEGF-A. CONCLUSIONS: Our data demonstrate the distinct changes of N-glycan profile and the increase of sialylated N-glycans in eyes with NVG secondary to PDR.

Fetal Environment and Glycosylation Status in Neonatal Cord Blood
Ryosuke Sato, Kenji J. Tsuchiya, Hideo Matsuzaki, Nori Takei, Hiroaki Itoh, Naohiro Kanayama, Takafumi Suda, Hiroshi Watanabe, Tetsu Ohashi, Masakazu Tanaka, Shin-Ichiro Nishimura, Masato Maekawa
Medicine (United States), 95, 14, Lippincott Williams and Wilkins, 01 Apr. 2016, [Peer-reviewed]
English, Scientific journal

Synthetic Human NOTCH1 EGF Modules Unraveled Molecular Mechanisms for the Structural and Functional Roles of Calcium Ions and O-Glycans in the Ligand-Binding Region
Shun Hayakawa, Ryosuke Koide, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 55, 5, 776, 787, Feb. 2016, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Solid-phase synthesis of C-mannosylated glycopeptide on WSXWS motif of human erythropoietin receptor
Hiroshi Hinou, Yuya Abe, Shun Hayakawa, Kentaro Naruchi, Naoki Fujitani, Shin-Ichiro Nishimura
TETRAHEDRON LETTERS, 57, 7, 791, 795, Feb. 2016, [Peer-reviewed]
English, Scientific journal

Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates
R. S. Tan, H. Hinou, S.-I. Nishimura
RSC Advances, 6, 56, 50833, 50836, Royal Society of Chemistry (RSC), 2016, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal,

We uncovered β-galactosynthase–β-mannosynthase dual-activity of β-galactosidase (A. oryzae) that could revolutionize chemoenzymatic glycan and NDOs syntheses.

Effects of the multiple O-glycosylation states on antibody recognition of the immunodominant motif in MUC1 extracellular tandem repeats
Shobith Rangappa, Gerard Artigas, Risho Miyoshi, Yasuhiro Yokoi, Shun Hayakawa, Fayna Garcia-Martin, Hiroshi Hinou, Shin-Ichiro Nishimura
MEDCHEMCOMM, 7, 6, 1102, 1122, 2016, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Alteration of serum N-glycan profile in patients with autoimmune pancreatitis.　　　　　　　　　　　　　　　
Tomoda T, Nouso K, Kato H, Miyahara K, Dohi C, Morimoto Y, Kinugasa H, Akimoto Y, Matsumoto K, Yamamoto N, Noma Y, Horiguchi S, Tsutsumi K, Amano M, Nishimura SI, Yamamoto K
Pancreatology, 16, 1, 44, 51, 2016, [Peer-reviewed]
English, Scientific journal

Protein oxidation mediated by heme-induced active site conversion specific for heme-regulated transcription factor, iron response regulator
Kitatsuji, C, Izumi, K, Nambu, S, Kurogochi, M, Uchida, T, Nishimura, S.-I, Iwai, K, O’Brian, M. R, Ikeda-Saito, M, Ishimori, K
Scientific Reports, 6, 18703, 18703, Nature Publishing Group, Jan. 2016, [Peer-reviewed]
English, Scientific journal, The Bradyrhizobium japonicum transcriptional regulator Irr (iron response regulator) is a key regulator of the iron homeostasis, which is degraded in response to heme binding via a mechanism that involves oxidative modification of the protein. Here, we show that heme-bound Irr activates O-2 to form highly reactive oxygen species (ROS) with the "active site conversion" from heme iron to non-heme iron to degrade itself. In the presence of heme and reductant, the ROS scavenging experiments show that Irr generates H2O2 from O-2 as found for other hemoproteins, but H2O2 is less effective in oxidizing the peptide, and further activation of H2O2 is suggested. Interestingly, we find a time-dependent decrease of the intensity of the Soret band and appearance of the characteristic EPR signal at g = 4.3 during the oxidation, showing the heme degradation and the successive formation of a non-heme iron site. Together with the mutational studies, we here propose a novel "two-step self-oxidative modification" mechanism, during which O-2 is activated to form H2O2 at the heme regulatory motif (HRM) site and the generated H2O2 is further converted into more reactive species such as OH at the non-heme iron site in the His-cluster region formed by the active site conversion.

Large-scale Glycomics of Livestock: Discovery of Highly Sensitive Serum Biomarkers Indicating an Environmental Stress Affecting Immune Responses and Productivity of Holstein Dairy Cows
Rehan IF, Ueda K, Mitani T, Amano M, Hinou H, Ohashi T, Kondo S, Nishimura SI
J. Agricul. Food Chem., 63, 48, 10578, 10590, Dec. 2015, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells
Udono M, Fujii K, Harada G, Tsuzuki Y, Kadooka K, Zhang P, Fujii H, Amano M, Nishimura S-I, Tashiro K, Kuhara S, Katakura Y
Scientific Reports, 5, 17342, 17342, Dec. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, Many genes and signaling pathways have been found to be involved in cellular senescence program. In the present study, we have identified 16 senescence-associated genes by differential proteomic analysis of the normal human diploid fibroblast cell line, TIG-1, and focused on ATP6V0A2. The aim of this study is to clarify the role of ATP6V0A2, the causal gene for ARCL2, a syndrome of abnormal glycosylation and impaired Golgi trafficking, in cellular senescence program. Here we showed that ATP6V0A2 is critical for cellular senescence; impaired expression of ATP6V0A2 disperses the Golgi structure and triggers senescence, suggesting that ATP6V0A2 mediates these processes. FITC-lectin staining and glycoblotting revealed significantly different glycosylation structures in presenescent (young) and senescent (old) TIG-1 cells; reducing ATP6V0A2 expression in young TIG-1 cells yielded structures similar to those in old TIG-1 cells. Our results suggest that senescence-associated impaired expression of ATP6V0A2 triggers changes in Golgi structure and glycosylation in old TIG-1 cells, which demonstrates a role of ATP6V0A2 in cellular senescence program.

The Quest for Anticancer Vaccines: Deciphering the Fine-Epitope Specificity of Cancer-Related Monoclonal Antibodies by Combining Microarray Screening and Saturation Transfer Difference NMR.
Coelho H, Matsushita T, Artigas G, Hinou H, Cañada FJ, Lo-Man R, Leclerc C, Cabrita EJ, Jiménez-Barbero J, Nishimura S, Garcia-Martín F, Marcelo F
Journal of the American Chemical Society, 137, 39, 12438, 12441, Oct. 2015, [Peer-reviewed]

A comprehensive glycome profiling of Huntington's disease transgenic mice.
Gizaw ST, Koda T, Amano M, Kamimura K, Ohashi T, Hinou H, Nishimura S
Biochimica et biophysica acta, 1850, 9, 1704, 1718, Sep. 2015, [Peer-reviewed], [Last author, Corresponding author]

Glycome characterization of immunoglobulin G from buffalo (Bubalus bubalis ) colostrum
Mamatha Bhanu L.S, Amano M, Nishimura S-I, Aparna H.S
Glycoconj. J., 32, 625, 634, Aug. 2015, [Peer-reviewed]
English, Scientific journal

Alteration of N-Glycan Profiles in Diabetic Retinopathy.
Inafuku S, Noda K, Amano M, Ohashi T, Yoshizawa C, Saito W, Murata M, Kanda A, Nishimura S, Ishida S
Investigative ophthalmology & visual science, 56, 9, 5316, 5322, Aug. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, PURPOSE: To investigate the alteration of vitreal N-glycans in patients with proliferative diabetic retinopathy (PDR). METHODS: Plasma and vitreous samples were collected from 17 patients (10 females and 7 males) with PDR (PDR group) and 17 nondiabetic patients (8 females and 9 males) with epiretinal membrane (ERM) and idiopathic macular hole (MH) (non-diabetes mellitus [DM] group). Profiles of N-glycans were analyzed by a glycoblotting-based high-throughput protocol that we recently developed. Human retinal microvascular endothelial cells (HRMECs) were cultivated with culture media containing either low glucose (5 mM) or high glucose (25 mM), and expression levels of sialyltransferases were analyzed by real-time PCR and ELISA. RESULTS: Amount of N-glycans in the vitreous fluid of the PDR group was significantly higher than that of the non-DM group (495.5 ± 37.4 vs. 142.7 ± 30.8 pmol/100 μg protein, P < 0.005), whereas there was no significant difference in the plasma samples between the PDR and the non-DM group. In addition, profile analysis showed that N-glycans with sialic acids increased in the vitreous of the PDR group (328.4 ± 25.8 pmol/100 μg protein) compared to the non-DM group (92.1 ± 21.2 pmol/100 μg protein, P < 0.0005). Expression levels of sialyltransferases ST3GAL1 and ST3GAL4 were upregulated in the HRMECs after high-glucose stimulation. Consistent with the real-time PCR data, high-glucose stimulation elevated the protein levels of ST3GAL1 (117.4 ± 14.9 pg/mg, P < 0.01) and ST3GAL4 (6.1 ± 0.9 pg/mg, P < 0.05) in the HRMECs compared with the cells cultured with low-glucose culture media (ST3GAL1, 64.4 ± 5.8 pg/mg; ST3GAL4, 3.8 ± 0.3 pg/mg). CONCLUSIONS: Our data demonstrate distinct changes in the N-glycan profile and an increase in sialylated N-glycans in eyes with PDR.

Serum N-glycan profiles in patients with intraductal papillary mucinous neoplasms of the pancreas.
Akimoto Y, Nouso K, Kato H, Miyahara K, Dohi C, Morimoto Y, Kinugasa H, Tomoda T, Yamamoto N, Tsutsumi K, Kuwaki K, Onishi H, Ikeda F, Nakamura S, Shiraha H, Takaki A, Okada H, Amano M, Nishimura S, Yamamoto K
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 15, 4, 432, 438, Jul. 2015, [Peer-reviewed]

Rapid endolysosomal escape and controlled intracellular trafficking of cell surface mimetic quantum dots-anchored peptides and glycopeptides.
Tan RS, Naruchi K, Amano M, Hinou H, Nishimura SI
ACS Chemical Biology, 10, 9, 2073, 2086, Jul. 2015, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Prognostic value of altered N-glycosylation of circulating glycoproteins in patients with unresectable pancreatic cancer treated with gemcitabine.
Koji Miyahara, Kazuhiro Nouso, Yuki Morimoto, Hideaki Kinugasa, Hironari Kato, Naoki Yamamoto, Koichiro Tsutsumi, Kenji Kuwaki, Hideki Onishi, Fusao Ikeda, Shinichiro Nakamura, Hidenori Shiraha, Akinobu Takaki, Taku Nakahara, Yoshiaki Miura, Hidehisa Asada, Maho Amano, Shin-Ichiro Nishimura, Kazuhide Yamamoto
Pancreas, 44, 4, 551, 556, May 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVES: The objectives of this study were to examine the whole-serum N-glycan profile of patients with unresectable pancreatic cancer and to evaluate the ability of glycans to predict gemcitabine treatment efficacy and patient survival. METHODS: We collected serum from 52 patients with advanced pancreatic cancer before they began gemcitabine monotherapy. The serum glycan profile was measured through comprehensive quantitative high-throughput glycome analysis and compared with the treatment efficacy and patient survival. RESULTS: Of the 61 glycans detected, the serum levels of glycan 4310 (molecular weight [m/z] 1549.566), 6301 (m/z 2032.724), and 9200 (m/z 2010.692) were high in patients with a short time to tumor progression (TTP). Multivariate analysis revealed that a high glycan 9200 concentration was an independent risk factor for shorter TTP (hazard ratio, 2.11; 95% confidence interval, 1.07-4.17) and poor overall survival (hazard ratio, 2.56; 95% confidence interval, 1.08-6.19). The median TTP of patients with up-regulation of 9200 after gemcitabine treatment was shorter than for the remaining patients (91 vs 301 days; P = 0.0005). A similar relationship was observed for overall survival (median, 181 vs 561 days; P = 0.001). CONCLUSIONS: Glycan 9200 is a possible biomarker predicting gemcitabine efficacy survival in patients with unresectable pancreatic cancer.

Use of non-invasive serum glycan markers to distinguish non-alcoholic steatohepatitis from simple steatosis.
Yamasaki Y, Nouso K, Miyahara K, Wada N, Dohi C, Morimoto Y, Kinugasa H, Takeuchi Y, Yasunaka T, Kuwaki K, Onishi H, Ikeda F, Miyake Y, Nakamura S, Shiraha H, Takaki A, Iwasaki Y, Amano M, Nishimura S, Yamamoto K
Journal of gastroenterology and hepatology, 30, 3, 528, 534, 3, Mar. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND AND AIMS: Serum glycans have been reported to be promising diagnostic markers for many inflammatory diseases and cancers. The aims of this study were to investigate whole glycan expression in patients with non-alcoholic fatty liver diseases and to evaluate the potential use of glycan profiles as new clinical biomarkers to distinguish non-alcoholic steatohepatitis (NASH) from simple steatosis (SS). METHODS: We collected sera from 42 histologically proven NASH and 15 SS patients prior to treatment. Serum glycan profiles were measured by comprehensive, quantitative, high-throughput glycome analysis, and diagnostic values of serum glycans for NASH prediction were examined. RESULTS: Among the 41 serum glycans examined, the expression levels of 8 glycans in NASH were significantly higher than those of SS. Out of these eight glycans, three glycans (m/z 1955, 2032, and 2584) showed high areas under the receiver operating characteristic curve (0.833, 0.863, and 0.866, respectively) for distinguishing NASH from SS. In multivariate analyses with clinical parameters and serum glycans, these three glycans were significant predictive factors for distinguishing NASH from SS. The odds ratio of m/z 1955, 2032, and 2584 were 48.5, 6.46, and 11.8, respectively. These glycans also correlated significantly with lobular inflammation, ballooning, and fibrosis, but not with steatosis. CONCLUSION: We clearly demonstrated whole-serum glycan profiles in NASH patients, and the feasibility of serum glycans (m/z 1955, 2032, and 2584) as new noninvasive biomarkers for distinguishing NASH from SS.

Application of glyco-blotting for identification of structures of polysaccharides causing membrane fouling in a pilot-scale membrane bioreactor treating municipal wastewater
Kimura Katsuki, Nishimura Shin-Ichiro, Miyoshi Risho, Hoque Asiful, Miyoshi Taro, Watanabe Yoshimasa
BIORESOURCE TECHNOLOGY, 179, 180, 186, Mar. 2015, [Peer-reviewed]

Nutriglycomics in Mice Fed High-Fat or Low-Protein Diets　　　　　　　　　　　　　　　
Watanabe N, Hara Y, Kono A, Ohashi T, Amano M, Nishimura S-I
Journal of Nutrition & Metabolism, 1, 4, 8, Dec. 2014, [Peer-reviewed]

Delineating Binding Modes of Gal/GalNAc and Structural Elements of the Molecular Recognition of Tumor-Associated Mucin Glycopeptides by the Human Macrophage Galactose-Type Lectin
Filipa Marcelo, Fayna Garcia-Martin, Takahiko Matsushita, Joao Sardinha, Helena Coelho, Anneloes Oude-Vrielink, Christiane Koller, Sabine Andre, Eurico J. Cabrita, Hans-Joachim Gabius, Shin-Ichiro Nishimura, Jesus Jimenez-Barbero, F. Javier Canada
CHEMISTRY-A EUROPEAN JOURNAL, 20, 49, 16147, 16155, Dec. 2014, [Peer-reviewed]
English, Scientific journal

Alteration of N-glycan profiles in patients with chronic hepatitis and hepatocellular carcinoma.
Miyahara K, Nouso K, Dohi C, Morimoto Y, Kinugasa H, Wada N, Takeuchi Y, Kuwaki K, Onishi H, Ikeda F, Miyake Y, Nakamura S, Shiraha H, Takaki A, Amano M, Nishimura SI, Yamamoto K
Hepatology research : the official journal of the Japan Society of Hepatology, 45, 9, 986, 993, Dec. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal, AIM: Most of the modification of N-glycosylation reported in cancers including hepatocellular carcinoma (HCC) were based on the examinations of a small number of patients or particular proteins. The aim of this study is to reveal changes in whole serum N-glycan profiles systematically during the process of hepatocarcinogenesis and to elucidate their clinical application. METHODS: We analyzed sera from 105 patients with chronic hepatitis/liver cirrhosis (CH/LC) and age-/sex-matched healthy volunteers (HLT), as well as from 114 patients with HCC. Serum N-glycan profiles were measured comprehensively by a new, quantitative, high-throughput method and compared with clinical parameters. RESULTS: The total amount of N-glycan expression was significantly higher in patients with CH/LC than in HLT; however, no differences were observed between CH/LC and HCC patients. In HCC patients, multi-antennary glycans with fucose residues, particularly m/z 3195, were increased compared with CH/LC patients. The expression of m/z 3195 was high, especially in patients with a high number of intrahepatic lesions (>3), large tumor size (>3 cm), macroscopic vascular invasion or metastasis. The ratio of pairs of glycans on the same path of the biosynthesis pathway (m/z 3195/1914) showed a higher area under the receiver-operator curve of 0.810 than any other single glycan to distinguish HCC from CH/LC. CONCLUSION: We demonstrate the full spectrum of the alterations of serum N-glycans comprehensively in patients with liver disease, and elucidate the possible use of glycans as novel biomarkers of liver disease progression.

Fast Epitope Mapping for the Anti-MUC1 Monoclonal Antibody by Combining a One-Bead-One-Glycopeptide Library and a Microarray Platform
Fayna Garcia-Martin, Takahiko Matsushita, Hiroshi Hinou, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 20, 48, 15891, 15902, Nov. 2014, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

SerumTri- and Tetra-Antennary N-Glycan is a Potential Predictive Biomarker for Castration-Resistant Prostate Cancer
Yusuke Ishibashi, Yuki Tobisawa, Shingo Hatakeyama, Tetsu Ohashi, Masakazu Tanaka, Shintaro Narita, Takuya Koie, Tomonori Habuchi, Shin-Ichiro Nishimura, Chikara Ohyama, Tohru Yoneyama
PROSTATE, 74, 15, 1521, 1529, Nov. 2014, [Peer-reviewed]
English, Scientific journal

Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation
Michiyo Terashima, Maho Amano, Tomohiro Onodera, Shin-Ichiro Nishimura, Norimasa Iwasaki
STEM CELL RESEARCH, 13, 3, 454, 464, Nov. 2014, [Peer-reviewed]
English, Scientific journal

A comparison of N-glycan profiles in human plasma and vitreous fluid.
Inafuku S, Noda K, Amano M, Ohashi T, Yoshizawa C, Saito W, Kanda A, Nishimura S, Ishida S
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 252, 8, 1235, 1243, Aug. 2014, [Peer-reviewed]

Serum N-Glycan Alteration Associated with Renal Cell Carcinoma Detected by High Throughput Glycan Analysis
Shingo Hatakeyama, Maho Amano, Yuki Tobisawa, Tohru Yoneyama, Norihiko Tsuchiya, Tomonori Habuchi, Shin-Ichiro Nishimura, Chikara Ohyama
JOURNAL OF UROLOGY, 191, 3, 805, 813, Mar. 2014, [Peer-reviewed]
English, Scientific journal

A straightforward protocol for the preparation of high performance microarray displaying synthetic MUC1 glycopeptides
Takahiko Matsushita, Wataru Takada, Kota Igarashi, Kentaro Naruchi, Risho Miyoshi, Fayna Garcia-Martin, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1840, 3, 1105, 1116, Mar. 2014, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Synthesis of neoglycosphingolipid from methoxyamino-functionalized ceramide
Junya Ishida, Hiroshi Hinou, Kentaro Naruchi, Shin-Ichiro Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 24, 4, 1197, 1200, Feb. 2014, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Serum Glycan as a Prognostic Marker in Patients With Advanced Hepatocellular Carcinoma Treated With Sorafenib
Koji Miyahara, Kazuhiro Nouso, Yasuhiro Miyake, Shinichiro Nakamura, Shuntaro Obi, Maho Amano, Kazuko Hirose, Shin-ichiro Nishimura, Kazuhide Yamamoto
HEPATOLOGY, 59, 1, 355, 356, Jan. 2014, [Peer-reviewed]
English

Allying with armored snails: the complete genome of gammaproteobacterial endosymbiont
Satoshi Nakagawa, Shigeru Shimamura, Yoshihiro Takaki, Yohey Suzuki, Shun-ichi Murakami, Tamaki Watanabe, So Fujiyoshi, Sayaka Mino, Tomoo Sawabe, Takahiro Maeda, Hiroko Makita, Suguru Nemoto, Shin-Ichiro Nishimura, Hiromi Watanabe, Tomo-O Watsuji, Ken Takai
ISME JOURNAL, 8, 1, 40, 51, Jan. 2014, [Peer-reviewed]
English, Scientific journal

Potential usage for in vivo lectin screening in live animals utilizing cell surface mimetic glyco-nanoparticles, phosphorylcholine-coated quantum dots (PC-QDs)
Maho Amano, Hiroshi Hinou, Risho Miyoshi, Shin-Ichiro Nishimura
Methods in Molecular Biology, 1200, 361, 369, Humana Press Inc., 2014, [Peer-reviewed], [Invited]
English, Scientific journal

Discovery of novel differentiation markers in the early stage of chondrogenesis by glycoform-focused reverse proteomics and genomics
Takeshi Ishihara, Kiyoshi Kakiya, Koji Takahashi, Hiroto Miwa, Masatomo Rokushima, Tomoyo Yoshinaga, Yoshikazu Tanaka, Takaomi Ito, Hiroko Togame, Hiroshi Takemoto, Maho Amano, Norimasa Iwasaki, Akio Minami, Shin-Ichiro Nishimura
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1840, 1, 645, 655, Jan. 2014, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

A new approach for the synthesis of hyperbranched N-glycan core structures from locust bean gum.
Ravi Kumar HV, Naruchi K, Miyoshi R, Hinou H, Nishimura S
Organic letters, 15, 24, 6278, 6281, 20 Dec. 2013, [Peer-reviewed], [Last author, Corresponding author]

Serum Glycan Markers for Evaluation of Disease Activity and Prediction of Clinical Course in Patients with Ulcerative Colitis
Koji Miyahara, Kazuhiro Nouso, Shunsuke Saito, Sakiko Hiraoka, Keita Harada, Sakuma Takahashi, Yuki Morimoto, Sayo Kobayashi, Fusao Ikeda, Yasuhiro Miyake, Hidenori Shiraha, Akinobu Takaki, Hiroyuki Okada, Maho Amano, Kazuko Hirose, Shin-Ichiro Nishimura, Kazuhide Yamamoto
PLoS ONE, 8, 10, e74861, 10, 07 Oct. 2013, [Peer-reviewed]
English, Scientific journal

Clinical utility of high-throughput glycome analysis in patients with pancreatic cancer
Kazuhiro Nouso, Maho Amano, Yoichi M. Ito, Koji Miyahara, Yuki Morimoto, Hironari Kato, Koichiro Tsutsumi, Takeshi Tomoda, Naoki Yamamoto, Shinichiro Nakamura, Sayo Kobayashi, Kenji Kuwaki, Hiroaki Hagihara, Hideki Onishi, Yasuhiro Miyake, Fusao Ikeda, Hidenori Shiraha, Akinobu Takaki, Taku Nakahara, Shin-Ichiro Nishimura, Kazuhide Yamamoto
JOURNAL OF GASTROENTEROLOGY, 48, 10, 1171, 1179, Oct. 2013, [Peer-reviewed]
English, Scientific journal

A novel approach for the parallel synthesis of glycopeptides by combining solid-phase peptide synthesis and dendrimer-supported enzymatic modifications
MATSUSHITA Takahiko, HANDA Seiji, NARUCHI Kentaro, GARCIA-MARTIN Fayna, HINOU Hiroshi, NISHIMURA Shin-Ichiro
Polymer journal, 45, 8, 854, 862, Nature Publishing Group, Aug. 2013, [Invited], [Last author, Corresponding author]
English, Scientific journal

血清中糖鎖の網羅的解析による肝細胞癌新規バイオマーカーの開発　　　　　　　　　　　　　　　
神山 俊哉, 柿坂 達彦, 横尾 英樹, 蒲池 浩文, 若山 顕治, 敦賀 陽介, 三浦 信明, 西村 紳一郎, 藤堂 省, 武冨 紹信
日本消化器外科学会総会, 68回, O, 5, (一社)日本消化器外科学会, Jul. 2013
Japanese

Identification of novel serum biomarkers of hepatocellular carcinoma using glycomic analysis
Toshiya Kamiyama, Hideki Yokoo, Jun-Ichi Furukawa, Masaki Kurogochi, Tomoaki Togashi, Nobuaki Miura, Kazuaki Nakanishi, Hirofumi Kamachi, Tatsuhiko Kakisaka, Yosuke Tsuruga, Masato Fujiyoshi, Akinobu Taketomi, Shin-Ichiro Nishimura, Satoru Todo
HEPATOLOGY, 57, 6, 2314, 2325, Jun. 2013, [Peer-reviewed]
English, Scientific journal

Attomolar Detection of Influenza A Virus Hemagglutinin Human H1 and Avian H5 Using Glycan-Blotted Field Effect Transistor Biosensor
Sho Hideshima, Hiroshi Hinou, Daisuke Ebihara, Ryosuke Sato, Shigeki Kuroiwa, Takuya Nakanishi, Shin-Ichiro Nishimura, Tetsuya Osaka
ANALYTICAL CHEMISTRY, 85, 12, 5641, 5644, Jun. 2013, [Peer-reviewed]
English, Scientific journal

潰瘍性大腸炎の活動性・予後予測における血清糖鎖マーカーの有用性　　　　　　　　　　　　　　　
宮原 孝治, 能祖 一裕, 平岡 佐規子, 森元 裕貴, 高橋 索真, 小林 沙代, 斎藤 俊介, 原田 馨太, 山本 和秀, 天野 麻穂, 西村 紳一郎
日本臨床分子医学会学術総会プログラム・抄録集, 50回, 76, 76, 日本臨床分子医学会, Apr. 2013
Japanese

Microwave-Assisted Solid-Phase Synthesis of Antifreeze Glycopeptides
Ryukou Izumi, Takahiko Matsushita, Naoki Fujitani, Kentaro Naruchi, Hiroki Shimizu, Sakae Tsuda, Hiroshi Hinou, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 19, 12, 3913, 3920, Mar. 2013, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycoblotting-based high throughput protocol for the structural characterization of hyaluronan degradation products during enzymatic fragmentation
Takayuki Furukawa, Misaki Arai, Fayna Garcia-Martin, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura
GLYCOCONJUGATE JOURNAL, 30, 2, 171, 182, Feb. 2013, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses
Masatoshi Okamatsu, Fei Feng, Tatsuya Ohyanagi, Noriko Nagahori, Kazuhiko Someya, Yoshihiro Sakoda, Nobuaki Miura, Shin-Ichiro Nishimura, Hiroshi Kida
JOURNAL OF VIROLOGICAL METHODS, 187, 2, 390, 394, Feb. 2013, [Peer-reviewed]
English, Scientific journal

Novel thiosialosides tethered to metal nanoparticles as potent influenza A virus haemagglutinin blockers.
Feng F, Sakoda Y, Ohyanagi T, Nagahori N, Shibuya H, Okamastu M, Miura N, Kida H, Nishimura SI
Antiviral therapy, Feb. 2013, [Peer-reviewed]

Effect of Ganglioside GM3 Synthase Gene Knockout on the Glycoprotein N-Glycan Profile of Mouse Embryonic Fibroblast
Noriko Nagahori, Tadashi Yamashita, Maho Amano, Shin-Ichiro Nishimura
CHEMBIOCHEM, 14, 1, 73, 82, Jan. 2013, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Macrocyclic Mechanism-Based Inhibitor for Neuraminidases
Hirokazu Kai, Hiroshi Hinou, Kentaro Naruchi, Takahiko Matsushita, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 19, 4, 1364, 1372, Jan. 2013, [Peer-reviewed]
English, Scientific journal

Site-Specific Conformational Alteration Induced by Sialylation of MUC1 Tandem Repeating Glycopeptides at an Epitope Region for the Anti-KL-6 Monoclonal Antibody
Takahiko Matsushita, Naoki Ohyabu, Naoki Fujitani, Kentaro Naruchi, Hiroki Shimizu, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 52, 2, 402, 414, Jan. 2013, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Novel thiosialosides tethered to metal nanoparticles as potent influenza A virus haemagglutinin blockers
Fei Feng, Yoshihiro Sakoda, Tatsuya Ohyanagi, Noriko Nagahori, Hitomi Shibuya, Masatoshi Okamastu, Nobuaki Miura, Hiroshi Kida, Shin-Ichiro Nishimura
Antiviral Chemistry and Chemotherapy, 23, 2, 59, 65, 2, 2013, [Peer-reviewed]
English, Scientific journal

N- and O-glycome analysis of serum and urine from bladder cancer patients using a high-throughput glycoblotting method
Motoi Takeuchi, Maho Amano, Hiroshi Kitamura, Taiji Tsukamoto, Naoya Masumori, Kazuko Hirose, Tetsu Ohashi, Shin-Ichiro Nishimura
Journal of Glycomics and Lipidomics, 3, 1, 108, 2013, [Peer-reviewed]
English, Scientific journal

Serum N-glycan profiling predicts prognosis in patients undergoing hemodialysis.
Hatakeyama S, Amano M, Tobisawa Y, Yoneyama T, Tsushima M, Hirose K, Yoneyama T, Hashimoto Y, Koie T, Saitoh H, Yamaya K, Funyu T, Nishimura S, Ohyama C
TheScientificWorldJournal, 2013, 268407, 2013, [Peer-reviewed]

Further examination of polysaccharides causing membrane fouling in membrane bioreactors (MBRs): Application of lectin affinity chromatography and MALDI-TOF/MS
Katsuki Kimura, Ippei Tanaka, Shin-Ichiro Nishimura, Risho Miyoshi, Taro Miyoshi, Yoshimasa Watanabe
WATER RESEARCH, 46, 17, 5725, 5734, Nov. 2012, [Peer-reviewed]
English, Scientific journal

Tumour suppressor p16INK4a-anoikis-favouring decrease in N/O-glycan/cell surface sialylation by down-regulation of enzymes in sialic acid biosynthesis in tandem in a pancreatic carcinoma model
Maho Amano, Hanna Eriksson, Joachim C. Manning, Katharina M. Detjen, Sabine Andre, Shin-Ichiro Nishimura, Janne Lehtio, Hans-Joachim Gabius
FEBS JOURNAL, 279, 21, 4062, 4080, Nov. 2012, [Peer-reviewed]
English

Molecular shuttle between extracellular and cytoplasmic space allows for monitoring of GAG biosynthesis in human articular chondrocytes.
Hoshi H, Shimawaki K, Takegawa Y, Ohyanagi T, Amano M, Hinou H, Nishimura S
Biochimica et biophysica acta, 1820, 1391, 1398, 9, Sep. 2012, [Peer-reviewed], [Last author, Corresponding author]

Determination of O-Glycosylation Heterogeneity Using a Mass-Spectrometric Method Retaining Sugar Modifications
Naomi Manri, Yasuhiro Takegawa, Naoki Fujitani, Akihito Kaneko, Atsumu Hirabayashi, Shin-Ichiro Nishimura, Takeshi Sakamoto
ANALYTICAL SCIENCES, 28, 7, 723, 727, Jul. 2012, [Peer-reviewed]
English, Scientific journal

Aglycone-focused randomization of 2-difluoromethylphenyl-type sialoside suicide substrates for neuraminidases
Hirokazu Kai, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY, 20, 8, 2739, 2746, Apr. 2012, [Peer-reviewed]
English, Scientific journal

Strict Stereocontrol by 2,4-O-Di-tert-butylsilylene Group on beta-Glucuronylations
Takayuki Furukawa, Hiroshi Hinou, Shin-Ichiro Nishimura
ORGANIC LETTERS, 14, 8, 2102, 2105, Apr. 2012, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Elucidation of the sugar recognition ability of the lectin domain of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 by using unnatural glycopeptide substrates
Yayoi Yoshimura, Aaron S. Nudelman, Steven B. Levery, Hans H. Wandall, Eric P. Bennett, Ole Hindsgaul, Henrik Clausen, Shin-Ichiro Nishimura
GLYCOBIOLOGY, 22, 3, 429, 438, Mar. 2012, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Phosphorylcholine Self-Assembled Monolayer-Coated Quantum Dots: Real-Time Imaging of Live Animals by Cell Surface Mimetic Glyco-Nanoparticles
Shin-Ichiro Nishimura
CLINICS IN LABORATORY MEDICINE, 32, 1, 73, +, Mar. 2012, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

A cross-linked polymer possessing a high density of hydrazide groups: high-throughput glycan purification and labeling for high-performance liquid chromatography analysis
Midori Abe, Hideyuki Shimaoka, Masao Fukushima, Shin-Ichiro Nishimura
POLYMER JOURNAL, 44, 3, 269, 277, Mar. 2012, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Effects of Single Genetic Damage in Carbohydrate-Recognizing Proteins in Mouse Serum N-Glycan Profile Revealed by Simple Glycotyping Analysis
Maho Amano, Ryo Hashimoto, Shin-Ichiro Nishimura
CHEMBIOCHEM, 13, 3, 451, 464, Feb. 2012, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

IFN-γ elevates airway hyper-responsiveness via up-regulation of neurokinin A/neurokinin-2 receptor signaling in a severe asthma model.
Minoru Kobayashi, Shigeru Ashino, Yasuo Shiohama, Daiko Wakita, Hidemitsu Kitamura, Takashi Nishimura
European journal of immunology, 42, 2, 393, 402, Feb. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

An efficient protocol for the solid-phase synthesis of glycopeptides under microwave irradiation
Fayna Garcia-Martin, Hiroshi Hinou, Takahiko Matsushita, Shun Hayakawa, Shin-Ichiro Nishimura
ORGANIC & BIOMOLECULAR CHEMISTRY, 10, 8, 1612, 1617, 2012, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycomics for Drug Discovery: Metabolic Perturbation in Androgen-Independent Prostate Cancer Cells Induced by Unnatural Hexosamine Mimics
Shin-Ichiro Nishimura, Megumi Hato, Satoshi Hyugaji, Fei Feng, Maho Amano
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51, 14, 3386, 3390, 2012, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

H-bonding promotion of peptide solubility and cyclization by fluorinated alcohols
Hiroshi Hinou, Kei Hyugaji, Fayna Garcia-Martin, Shin-Ichiro Nishimura, Fernando Albericio
RSC ADVANCES, 2, 7, 2729, 2731, 2012, [Peer-reviewed]
English, Scientific journal

Alterations of High-Mannose Type N-Glycosylation in Human and Mouse Osteoarthritis Cartilage
Atsushi Urita, Tomoya Matsuhashi, Tomohiro Onodera, Hiroaki Nakagawa, Megumi Hato, Maho Amano, Naoki Seito, Akio Minami, Shin-Ichiro Nishimura, Norimasa Iwasaki
ARTHRITIS AND RHEUMATISM, 63, 11, 3428, 3438, Nov. 2011, [Peer-reviewed]
English, Scientific journal

Alteration of the N-glycome of bovine milk glycoproteins during early lactation
Shota Takimori, Hideyuki Shimaoka, Jun-Ichi Furukawa, Tadashi Yamashita, Maho Amano, Naoki Fujitani, Yasuhiro Takegawa, Lennart Hammarstrom, Imre Kacskovics, Yasuro Shinohara, Shin-Ichiro Nishimura
FEBS JOURNAL, 278, 19, 3769, 3781, Oct. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

A potential glucuronate glycosyl donor with 2-O-acyl-6,3-lactone structure: efficient synthesis of glycosaminoglycan disaccharides
Takayuki Furukawa, Hiroshi Hinou, Ken Shimawaki, Shin-Ichiro Nishimura
Tetrahedron Letters, 52, 43, 5567, 5570, Elsevier BV, Oct. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Development of quantitative plasma N-glycoproteomics using label-free 2-D LC-MALDI MS and its applicability for biomarker discovery in hepatocellular carcinoma
Takeshi Ishihara, Isao Fukuda, Atsushi Morita, Yoshihiko Takinami, Hiroyuki Okamoto, Shin-Ichiro Nishimura, Yoshito Numata
JOURNAL OF PROTEOMICS, 74, 10, 2159, 2168, Sep. 2011, [Peer-reviewed]
English, Scientific journal

前立腺癌患者血清に含まれるAcute Phase Proteinの糖鎖構造解析　　　　　　　　　　　　　　　
藤村 務, 數野 彩子, 古川 潤一, 篠原 康郎, 西村 紳一郎, 藤目 真, 村山 季美枝
日本生化学会大会プログラム・講演要旨集, 84回, 4P, 0600, (公社)日本生化学会, Sep. 2011
Japanese

Toward Green and Sustainable Chemical Glycosylation: Enhanced Lewis Acidity of Recyclable Solid Super Acid Catalyst, SO4/ZrO2 by CaCl2 Doping
Hiroshi Hinou, Naohiro Saito, Takahiro Maeda, Masao Matsuda, Yuichi Kamiya, Shin-Ichiro Nishimura
Journal of Carbohydrate Chemistry, 30, 7-9, 575, 586, Informa UK Limited, 01 Sep. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Importance of Sialic Acid Residues Illuminated by Live Animal Imaging Using Phosphorylcholine Self-Assembled Monolayer-Coated Quantum Dots
Tatsuya Ohyanagi, Noriko Nagahori, Ken Shimawaki, Hiroshi Hinou, Tadashi Yamashita, Akira Sasaki, Takashi Jin, Toshihiko Iwanaga, Masataka Kinjo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133, 32, 12507, 12517, Aug. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Biomechanical and histologic evaluation of tissue engineered ligaments using chitosan and hyaluronan hybrid polymer fibers: A rabbit medial collateral ligament reconstruction model
Toru Irie, Tokifumi Majima, Naohiro Sawaguchi, Tadanao Funakoshi, Shin-ichiro Nishimura, Akio Minami
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 97A, 2, 111, 117, May 2011, [Peer-reviewed]
English, Scientific journal

Insight into Glycan Diversity and Evolutionary Lineage Based on Comparative Avio-N-glycomics and Sialic Acid Analysis of 88 Egg Whites of Galloanserae
Kazuko Hirose, Maho Amano, Ryo Hashimoto, Yuan Chuan Lee, Shin-Ichiro Nishimura
BIOCHEMISTRY, 50, 21, 4757, 4774, May 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

A Strategy for Neuraminidase Inhibitors Using Mechanism-Based Labeling Information
Hiroshi Hinou, Risho Miyoshi, Yasuaki Takasu, Hirokazu Kai, Masaki Kurogochi, Shingo Arioka, Xiao-Dong Gao, Nobuaki Miura, Naoki Fujitani, Shinya Omoto, Tomokazu Yoshinaga, Tamio Fujiwara, Takeshi Noshi, Hiroko Togame, Hiroshi Takemoto, Shin-Ichiro Nishimura
CHEMISTRY-AN ASIAN JOURNAL, 6, 4, 1048, 1056, Apr. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

An Efficient Approach for the Characterization of Mucin-Type Glycopeptides: The Effect of O-Glycosylation on the Conformation of Synthetic Mucin Peptides
Ryo Hashimoto, Naoki Fujitani, Yasuhiro Takegawa, Masaki Kurogochi, Takahiko Matsushita, Kentaro Naruchi, Naoki Ohyabu, Hiroshi Hinou, Xiao Dong Gao, Naomi Manri, Hiroyuki Satake, Akihito Kaneko, Takeshi Sakamoto, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 17, 8, 2393, 2404, Feb. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Membrane-Bound Stable Glycosyltransferases: Highly Oriented Protein Immobilization by a C-Terminal Cationic Amphipathic Peptide
Kentaro Naruchi, Shin-Ichiro Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 50, 6, 1328, 1331, 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

TOWARD AUTOMATED GLYCAN ANALYSIS
Shin-Ichiro Nishimura
ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, VOL 65, 65, 219, 271, 2011, [Peer-reviewed], [Lead author, Corresponding author]
English, In book

The extract of Japanese soybean, Kurosengoku activates the production of IL-12 and IFN-γ by DC or NK1.1(+) cells in a TLR4- and TLR2-dependent manner.
Sachi Tanaka, Shin-ichi Koizumi, Naoko Makiuchi, Yuka Aoyagi, Emi Quivy, Rieko Mitamura, Tsutomu Kano, Daiko Wakita, Kenji Chamoto, Hidemitsu Kitamura, Takashi Nishimura
Cellular immunology, 266, 2, 135, 42, 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Glycoblotting-Assisted O-Glycomics: Ammonium Carbamate Allows for Highly Efficient O-Glycan Release from Glycoproteins
Yoshiaki Miura, Kentaro Kato, Yasuhiro Takegawa, Masaki Kurogochi, Jun-ichi Furukawa, Yasuro Shinohara, Noriko Nagahori, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura
ANALYTICAL CHEMISTRY, 82, 24, 10021, 10029, Dec. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Sialic Acid-focused Quantitative Mouse Serum Glycoproteomics by Multiple Reaction Monitoring Assay
Masaki Kurogochi, Takahiko Matsushista, Maho Amano, Jun-ichi Furukawa, Yasuro Shinohara, Masato Aoshima, Shin-Ichiro Nishimura
MOLECULAR & CELLULAR PROTEOMICS, 9, 11, 2354, 2368, Nov. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Artificial Golgi Apparatus: Globular Protein-like Dendrimer Facilitates Fully Automated Enzymatic Glycan Synthesis
Takahiko Matsushita, Izuru Nagashima, Masataka Fumoto, Takashi Ohta, Kuriko Yamada, Hiroki Shimizu, Hiroshi Hinou, Kentaro Naruchi, Takaomi Ito, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132, 46, 16651, 16656, Nov. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Chemical Synthesis, Folding, and Structural Insights into O-Fucosylated Epidermal Growth Factor-like Repeat 12 of Mouse Notch-1 Receptor
Kazumi Hiruma-Shimizu, Kensaku Hosoguchi, Yan Liu, Naoki Fujitani, Takashi Ohta, Hiroshi Hinou, Takahiko Matsushita, Hiroki Shimizu, Ten Feizo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132, 42, 14857, 14865, Oct. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

A new class of mechanism-based inhibitors for Trypanosoma cruzi trans-sialidase and their influence on parasite virulence
Sebastiao T. Carvalho, Mauro Sola-Penna, Isadora A. Oliveira, Samuel Pita, Arlan S. Goncalves, Bianca C. Neves, Francisco R. Sousa, Leonardo Freire-de-Lima, Masaki Kurogochi, Hiroshi Hinou, Shin-Ichiro Nishimura, Lucia Mendonca-Previato, Jose O. Previato, Adriane R. Todeschini
GLYCOBIOLOGY, 20, 8, 1034, 1045, Aug. 2010, [Peer-reviewed]
English, Scientific journal

Identification of glycosylated exendin-4 analogue with prolonged blood glucose-lowering activity through glycosylation scanning substitution
Taichi Ueda, Takaomi Ito, Kazuyoshi Tomita, Hiroko Togame, Masataka Fumoto, Kenji Asakura, Takeo Oshima, Shin-Ichiro Nishimura, Kohji Hanasaki
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20, 15, 4631, 4634, Aug. 2010, [Peer-reviewed]
English, Scientific journal

An Efficient Approach to the Discovery of Potent Inhibitors against Glycosyltransferases
Kensaku Hosoguchi, Takahiro Maeda, Jun-ichi Furukawa, Yasuro Shinohara, Hiroshi Hinou, Mitsuaki Sekiguchi, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF MEDICINAL CHEMISTRY, 53, 15, 5607, 5619, Aug. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Unexpected Tolerance of Glycosylation by UDP-GalNAc:Polypeptide alpha-N-Acetylgalactosaminyltransferase Revealed by Electron Capture Dissociation Mass Spectrometry: Carbohydrate as Potential Protective Groups
Yayoi Yoshimura, Takahiko Matsushita, Naoki Fujitani, Yasuhiro Takegawa, Haruhiko Fujihira, Kentarou Naruchi, Xiao-Dong Gao, Naomi Manri, Takeshi Sakamoto, Kentaro Kato, Hiroshi Hinou, Shin-Ichiro Nishimura
BIOCHEMISTRY, 49, 28, 5929, 5941, Jul. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Effect of cyclic three-dimensional strain on cell proliferation and collagen synthesis of fibroblast-seeded chitosan-hyaluronan hybrid polymer fiber
Naohiro Sawaguchi, Tokifumi Majima, Tadanao Funakoshi, Kazumi Shimode, Kazuo Harada, Akio Minami, Shin-Ichiro Nishimura
JOURNAL OF ORTHOPAEDIC SCIENCE, 15, 4, 569, 577, Jul. 2010, [Peer-reviewed]
English, Scientific journal

Novel trivalent anti-influenza reagent
Fei Feng, Nobuaki Miura, Norikazu Isoda, Yoshihiro Sakoda, Masatoshi Okamatsu, Hiroshi Kida, Shin-Ichiro Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20, 12, 3772, 3776, Jun. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Functional network of glycan-related molecules: Glyco-Net in Glycoconjugate Data Bank
Ryo Hashimoto, Kazuko Hirose, Taku Sato, Nobuhiro Fukushima, Nobuaki Miura, Shin-Ichiro Nishimura
BMC SYSTEMS BIOLOGY, 4, 91, Jun. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Threshold in Stage-specific Embryonic Glycotypes Uncovered by a Full Portrait of Dynamic N-Glycan Expression during Cell Differentiation
Maho Amano, Misa Yamaguchi, Yasuhiro Takegawa, Tadashi Yamashita, Michiyo Terashima, Jun-ichi Furukawa, Yoshiaki Miura, Yasuro Shinohara, Norimasa Iwasaki, Akio Minami, Shin-ichiro Nishimura
MOLECULAR & CELLULAR PROTEOMICS, 9, 3, 523, 537, Mar. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Carbohydrate Moieties Contribute Significantly to the Physicochemical Properties of French Bean 7S Globulin Phaseolin
Aiko Kimura, Mary Rose G. Tandang-Silvas, Takako Fukuda, Cerrone Cabanos, Yasuhiro Takegawa, Maho Amano, Shin-Ichiro Nishimura, Yasuki Matsumura, Shigeru Utsumi, Nobuyuki Maruyama
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58, 5, 2923, 2930, Mar. 2010, [Peer-reviewed]
English, Scientific journal

Highly Oriented Recombinant Glycosyltransferases: Site-Specific Immobilization of Unstable Membrane Proteins by Using Staphylococcus aureus Sortase A
Takaomi Ito, Reiko Sadamoto, Kentaro Naruchi, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura
BIOCHEMISTRY, 49, 11, 2604, 2614, Mar. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Potent inhibitor scaffold against Trypanosoma cruzi trans-sialidase
Shingo Arioka, Masahiro Sakagami, Rie Uematsu, Hiroto Yamaguchi, Hiroko Togame, Hiroshi Takemoto, Hiroshi Hinou, Shin-ichiro Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY, 18, 4, 1633, 1640, Feb. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

An Essential Epitope of Anti-MUC1 Monoclonal Antibody KL-6 Revealed by Focused Glycopeptide Library
Naoki Ohyabu, Hiroshi Hinou, Takahiko Matsushita, Ryukou Izumi, Hiroki Shimizu, Keiko Kawamoto, Yoshito Numata, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131, 47, 17102, 17109, Dec. 2009, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Microwave Effect for Glycosylation Promoted by Solid Super Acid in Supercritical Carbon Dioxide
Hiroshi Hinou, Naohiro Saito, Masato Ogawa, Takahiko Maeda, Shin-Ichiro Nishimura
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 10, 12, 5285, 5295, Dec. 2009, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Functional Neoglycopeptides: Synthesis and Characterization of a New Class of MUC1 Glycoprotein Models Having Core 2-Based O-Glycan and Complex-Type N-Glycan Chains
Takahiko Matsushita, Reiko Sadamoto, Naoki Ohyabu, Hideki Nakata, Masataka Fumoto, Naoki Fujitani, Yasuhiro Takegawa, Takeshi Sakamoto, Masaki Kurogochi, Hiroshi Hinou, Hiroki Shimizu, Takaomi Ito, Kentarou Naruchi, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura
BIOCHEMISTRY, 48, 46, 11117, 11133, Nov. 2009, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Chemoenzymatic Synthesis of Glycosylated Glucagon-like Peptide 1: Effect of Glycosylation on Proteolytic Resistance and in Vivo Blood Glucose-Lowering Activity
Taichi Ueda, Kazuyoshi Tomita, Yoshihide Notsu, Takaomi Ito, Masataka Fumoto, Tomoaki Takakura, Hirofumi Nagatome, Akio Takimoto, Shin-Ichi Mihara, Hiroko Togame, Keiko Kawamoto, Takanori Iwasaki, Kenji Asakura, Takeo Oshima, Kohji Hanasaki, Shin-Ichiro Nishimura, Hirosato Kondo
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131, 17, 6237, 6245, May 2009, [Peer-reviewed]
English, Scientific journal

Identification of novel helper epitopes of MAGE-A4 tumour antigen: useful tool for the propagation of Th1 cells
T. Ohkuri, D. Wakita, K. Chamoto, Y. Togashi, H. Kitamura, T. Nishimura
BRITISH JOURNAL OF CANCER, 100, 7, 1135, 1143, Mar. 2009, [Peer-reviewed]
English, Scientific journal

Glycosylation Specific for Adhesion Molecules in Epidermis and Its Receptor Revealed by Glycoform-focused Reverse Genomics
Rie Uematsu, Yasuro Shinohara, Hiroaki Nakagawa, Masaki Kurogochi, Jun-ichi Furukawa, Yoshiaki Miura, Masashi Akiyama, Hiroshi Shimizu, Shin-Ichiro Nishimura
MOLECULAR & CELLULAR PROTEOMICS, 8, 2, 232, 244, Feb. 2009, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

MALDI-TOF Mass-Spectrometry-Based Versatile Method for the Characterization of Protein Kinases
Noriyasu Kondo, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 15, 6, 1413, 1421, 2009, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Structural and Functional Glycosphingolipidomics by Glycoblotting with an Aminooxy-Functionalized Gold Nanoparticle
Noriko Nagahori, Midori Abe, Shin-Ichiro Nishimura
BIOCHEMISTRY, 48, 3, 583, 594, Jan. 2009, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

One-pot synthesis of cyclic antifreeze glycopeptides
Masakazu Hachisu, Hiroshi Hinou, Manabu Takamichi, Sakae Tsuda, Shuhei Koshidaa, Shin-Ichiro Nishimura
CHEMICAL COMMUNICATIONS, 13, 1641, 1643, 2009, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Interaction between the C Termini of Alg13 and Alg14 Mediates Formation of the Active UDP-N-acetylglucosamine Transferase Complex
Xiao-Dong Gao, Satoru Moriyama, Nobuaki Miura, Neta Dean, Shin-Ichiro Nishimura
JOURNAL OF BIOLOGICAL CHEMISTRY, 283, 47, 32534, 32541, Nov. 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Crucial Role of Aspartic Acid at Position 265 in the CH2 Domain for Murine IgG2a and IgG2b Fc-Associated Effector Functions
Lucie Baudino, Yasuro Shinohara, Falk Nimmerjahn, Jun-Ichi Furukawa, Munehiro Nakata, Eduardo Martinez-Soria, Franz Petry, Jeffery V. Ravetch, Shin-Ichiro Nishimura, Shozo Izui
JOURNAL OF IMMUNOLOGY, 181, 9, 6664, 6669, Nov. 2008, [Peer-reviewed]
English, Scientific journal

Alterations in the glycoform of cisplatin-resistant human carcinoma cells are caused by defects in the endoplasmic reticulum-associated degradation system
Hiroaki Nakagawa, Miki Ohira, Shunji Hayashi, Shigeaki Abe, Shin Saito, Noriko Nagahori, Kenji Monde, Yasuro Shinohara, Naoki Fujitani, Hirosato Kondo, Shin-Ichi Akiyama, Akira Nakagawara, Shin-Ichiro Nishimura
Cancer Letters, 270, 2, 295, 301, Elsevier BV, Nov. 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

A novel beta-defensin structure: A potential strategy of big defensin for overcoming resistance by gram-positive bacteria
Takahide Kouno, Naoki Fujitani, Mineyuki Mizuguchi, Tsukasa Osaki, Shin-ichiro Nishimura, Shun-ichiro Kawabata, Tomoyasu Aizawa, Makoto Demura, Katsutoshi Nitta, Keiichi Kawan
BIOCHEMISTRY, 47, 40, 10611, 10619, Oct. 2008, [Peer-reviewed]
English, Scientific journal

Magnetic nanoparticles for improving cell invasion in tissue engineering
Takuro Sasaki, Norimasa Iwasaki, Kenji Kohno, Mikio Kishimoto, Tokifumi Majima, Shin-Ichiro Nishimura, Akio Minami
Journal of Biomedical Materials Research Part A, 86A, 4, 969, 978, Wiley, 15 Sep. 2008, [Peer-reviewed]
English, Scientific journal

Impact of a three amino acid deletion in the CH2 domain of murine IgG1 on Fc-associated effector functions
Lucie Baudino, Falk Nimmerjahn, Yasuro Shinohara, Jun-Ichi Furukawa, Franz Petry, J. Sjef Verbeek, Shin-Ichiro Nishimura, Jeffery V. Ravetch, Shozo Izui
JOURNAL OF IMMUNOLOGY, 181, 6, 4107, 4112, Sep. 2008, [Peer-reviewed]
English, Scientific journal

Detection of Carcinoembryonic Antigens Using a Surface Plasmon Resonance Biosensor
Fengyu Su, Chunye Xu, Minoru Taya, Kimie Murayama, Yasuro Shinohara, Shin-Ichiro Nishimura
Sensors, 8, 7, 4282, 4295, MDPI AG, 18 Jul. 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Development of mature cartilage constructs using novel three-dimensional porous scaffolds for enhanced repair of osteochondral defects
Yasuhiko Kasahara, Norimasa Iwasaki, Shintaro Yamane, Tatsuya Igarashi, Tokifumi Majima, Sachiko Nonaka, Kazuo Harada, Shin-Ichiro Nishimura, Akio Minami
Journal of Biomedical Materials Research Part A, 86A, 1, 127, 136, Wiley, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Alteration of N-glycans related to articular cartilage deterioration after anterior cruciate ligament transection in rabbits.
Matsuhashi T, Iwasaki N, Nakagawa H, Hato M, Kurogochi M, Majima T, Minami A, Nishimura SI
Osteoarthritis Cartilage., 16, 7, 772, 778, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Novel long-acting incretin mimetics glycosylated glucagon-like peptide 1 exert improved dipeptidyl peptidase IV resistance and potent anti-diabetic efficacy
Kenji Asakura, Kazuyoshi Tomita, Taichi Ueda, Takeo Oshima, Takanori Iwasaki, Takaomi Ito, Hirofumi Nagatome, Akio Takimoto, Shin-Ichi Mihara, Hiroko Togame, Kohji Hanasaki, Shin-Ichiro Nishimura, Hirosato Kondo
DIABETES, 57, A4, A4, Jun. 2008, [Peer-reviewed]
English

Comprehensive analysis of N-glycans in serum glycoproteins of subjects with high fasting glucose levels
Hiroshi Ohira, Shinji Sakaue, Naofumi Itoh, Jun-Ichi Furukawa, Yoshiaki Miura, Ichizo Tsujino, Shin-Ichiro Nishimura, Masaharu Nishimura
DIABETES, 57, A704, A704, Jun. 2008, [Peer-reviewed]
English

Two-dimensional hydrophilic interaction chromatography coupling anion-exchange and hydrophilic interaction columns for separation of 2-pyridylamino derivatives of neutral and sialylated N-glycans
Kisaburo Deguchi, Takuro Keira, Kuriko Yamada, Hiroki Ito, Yasuhiro Takegawa, Hiroaki Nakagawa, Shin-Ichiro Nishimura
JOURNAL OF CHROMATOGRAPHY A, 1189, 1-2, 169, 174, May 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Alg13p, the catalytic subunit of the endoplasmic reticulum UDP-GlcNAc glycosyltransferase, is a target for proteasomal degradation
Nicole Averbeck, Xiao-Dong Gao, Shin-Ichiro Nishimura, Neta Dean
MOLECULAR BIOLOGY OF THE CELL, 19, 5, 2169, 2178, May 2008, [Peer-reviewed]
English, Scientific journal

Chemical and enzymatic synthesis of neoglycolipids in the presence of cyclodextrins
Izuru Nagashima, Hiroki Shimizu, Takahiko Matsushita, Shin-Ichiro Nishimura
Tetrahedron Letters, 49, 21, 3413, 3418, Elsevier BV, May 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Profiling ofN- andO-glycopeptides of erythropoietin by capillary zwitterionic type of hydrophilic interaction chromatography/electrospray ionization mass spectrometry
Yasuhiro Takegawa, Hiroki Ito, Takuro Keira, Kisaburo Deguchi, Hiroaki Nakagawa, Shin-Ichiro Nishimura
Journal of Separation Science, 31, 9, 1585, 1593, Wiley, May 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Chromatographic deuterium isotope effects of derivatizedN-glycans andN-glycopeptides in a zwitterionic type of hydrophilic interaction chromatography
Yasuhiro Takegawa, Megumi Hato, Kisaburo Deguchi, Hiroaki Nakagawa, Shin-Ichiro Nishimura
Journal of Separation Science, 31, 9, 1594, 1597, Wiley, May 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

臨床検体情報をどう治療へむすびつけるか? 血清中糖鎖の網羅的解析による肝細胞癌新規バイオマーカーの開発
横尾 英樹, 三浦 嘉晃, 神山 俊哉, 中西 一彰, 濱口 純, 工藤 岳秋, 古川 潤一, 中川 隆公, 田原 宗徳, 中馬 誠, 髭 修平, 篠原 康郎, 尾崎 倫孝, 多田 光宏, 松下 通明, 浅香 正博, 西村 紳一郎, 藤堂 省
日本外科学会雑誌, 109, 臨増2, 144, 144, (一社)日本外科学会, Apr. 2008
Japanese

Comprehensive Approach to Structural and Functional Glycomics Based on Chemoselective Glycoblotting and Sequential Tag Conversion
Jun-ichi Furukawa, Yasuro Shinohara, Hiromitsu Kuramoto, Yoshiaki Miura, Hideyuki Shimaoka, Masaki Kurogochi, Mika Nakano, Shin-Ichiro Nishimura
Analytical Chemistry, 80, 4, 1094, 1101, American Chemical Society (ACS), Feb. 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

BlotGlycoABC™, an Integrated Glycoblotting Technique for Rapid and Large Scale Clinical Glycomics
Yoshiaki Miura, Megumi Hato, Yasuro Shinohara, Hiromitsu Kuramoto, Jun-ichi Furukawa, Masaki Kurogochi, Hideyuki Shimaoka, Mitsuhiro Tada, Kazuaki Nakanishi, Michitaka Ozaki, Satoru Todo, Shin-Ichiro Nishimura
Molecular & Cellular Proteomics, 7, 2, 370, 377, American Society for Biochemistry & Molecular Biology (ASBMB), Feb. 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycosylation status of haptoglobin in sera of patients with prostate cancer vs. benign prostate disease or normal subjects
Tsutomu Fujimura, Yasuro Shinohara, Berangere Tissot, Poh-Choo Pang, Masaki Kurogochi, Seiichi Saito, Yoichi Arai, Martin Sadilek, Kimie Murayama, Anne Dell, Shin-Ichiro Nishimura, Sen-Itiroh Hakomori
INTERNATIONAL JOURNAL OF CANCER, 122, 1, 39, 49, Jan. 2008, [Peer-reviewed]
English, Scientific journal

FRET-based direct and continuous monitoring of human fucosyltransferases activity: An efficient synthesis of versatile GDP-L-fucose derivatives from abundant D-galactose
Takahiro Maeda, Shin-Ichiro Nishimuira
CHEMISTRY-A EUROPEAN JOURNAL, 14, 2, 478, 487, 2008, [Peer-reviewed]
English, Scientific journal

Glycoconjugate Data Bank : Structures - an annotated glycan structure database and N-glycan primary structure verification service
Taku Nakahara, Ryo Hashimoto, Hiroaki Nakagawa, Kenji Monde, Nobuaki Miura, Shin-Ichiro Nishimura
NUCLEIC ACIDS RESEARCH, 36, D368, D371, Jan. 2008, [Peer-reviewed]
English, Scientific journal

Bacterial Surface Engineering Utilizing Gluosamine Phosphate Derivatives as Cell Wall Precursor Surrogates
Reiko Sadamoto, Takeshi Matsubayashi, Masataka Shimizu, Taichi Ueda, Shuhei Koshida, Toshiaki Koda, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 14, 33, 10192, 10195, 2008, [Peer-reviewed]
English, Scientific journal

Positive expressions of N-acetylglucosaminyltransferase-V (GnT-V) and β1-6 branching N-linked oligosaccharides in human testicular germ cells diminish during malignant transformation and progression
Atsushi Kyan, Noritaka Kamimura, Shigeru Hagisawa, Shingo Hatakeyama, Takuya Koie, Takahiro Yoneyama, Yoichi Arai, Hiroaki Nakagawa, Shin-Ichiro Nishimura, Eiji Miyoshi, Yasuhiro Hashimoto, Chikara Ohyama
International Journal of Oncology, Spandidos Publications, 01 Jan. 2008, [Peer-reviewed]
English, Scientific journal

Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes
Naofumi Itoh, Shinji Sakaue, Hiroaki Nakagawa, Masaki Kurogochi, Hiroshi Ohira, Kisaburo Deguchi, Shin-Ichiro Nishimura, Masaharu Nishimura
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 293, 4, E1069, E1077, Oct. 2007, [Peer-reviewed]
English, Scientific journal

Swainsonine reduces 5-fluorouracil tolerance in the multistage resistance of colorectal cancer cell lines.
Jun Hamaguchi, Hiroaki Nakagawa, Masato Takahashi, Takeaki Kudo, Naoya Kamiyama, Bailong Sun, Takahiro Oshima, Yuji Sato, Kisaburo Deguchi, Satoru Todo, Shin-Ichiro Nishimura
Molecular cancer, 6, 58, 58, 21 Sep. 2007, [International Magazine]
English, Scientific journal, BACKGROUND: Drug resistance is a major problem in cancer chemotherapy. Acquisition of chemo-resistance not only reduces the effectiveness of drugs, but also promotes side effects and markedly reduces the patient's quality of life. However, a number of resistance mechanisms have been reported and are thought to be the reason for the difficulties in solving drug-resistance problems. RESULT: To investigate the mechanisms of drug resistance, a set of cell lines with different levels of sensitivity and possessing different mechanisms of resistance to 5-fluorouracil (5-FU) was established from a colorectal cancer cell line. The expression of thymidylate synthase, orotic acid phosphoribosyltransferase and dihydropyrimidine dehydrogenase, which are well known to be related to drug resistance, differed among these cell lines, indicating that these cell lines acquired different resistance mechanisms. However, swainsonine, an inhibitor of N-glycan biosynthesis, reduced 5-FU-tolerance in all resistant cells, whereas the sensitivity of the parental cells was unchanged. Further analysis of the N-glycan profiles of all cell lines showed partial inhibition of biosynthesis and no cytotoxicity at the swainsonine dosage tested. CONCLUSION: These observations suggest that N-linked oligosaccharides affect 5-FU resistance more widely than do drug-resistance related enzymes in colorectal cancer cells, and that the N-glycan could be a universal target for chemotherapy. Further, swainsonine may enhance the performance of chemotherapy by reducing tolerance.

Structural transition of a 15 amino acid residue peptide induced by GM1
Naoki Fujitani, Hiroki Shimizu, Teruhiko Matsubara, Takashi Ohta, Yuuki Komata, Nobuaki Miura, Toshinori Sato, Shin-Ichiro Nishimura
CARBOHYDRATE RESEARCH, 342, 12-13, 1895, 1903, Sep. 2007, [Peer-reviewed]
English, Scientific journal

Quantitative glycomics of human whole serum glycoproteins based on the standardized protocol for liberating N-glycans
Yoko Kita, Yoshiaki Miura, Jun-ichi Furukawa, Mika Nakano, Yasuro Shinohara, Masahiro Ohno, Akio Takimoto, Shin-lchiro Nishimura
MOLECULAR & CELLULAR PROTEOMICS, 6, 8, 1437, 1445, Aug. 2007, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Tumor suppressor p16(INK4a) - modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells
Sabine Andre, Hugo Sanchez-Ruderisch, Hiroaki Nakagawa, Malte Buchholz, Jurgen Kopitz, Pia Forberich, Wolfgang Kemmner, Corina Boeck, Kisaburo Deguchi, Katharia M. Detjen, Bertram Wiedenmann, Magnus von Knebel Doeberitz, Thomas M. Gress, Shin-Ichiro Nishimura, Stefan Rosewicz, Hans-Joachim Gabius
FEBS JOURNAL, 274, 13, 3233, 3256, Jul. 2007, [Peer-reviewed]
English

Effect of pore size on in vitro cartilage formation using chitosan-based hyaluronic acid hybrid polymer fibers
Shintaro Yamane, Norimasa Iwasaki, Yasuhiko Kasahara, Kazuo Harada, Tokifumi Majima, Kenji Monde, Shin-ichiro Nishimura, Akio Minami
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 81A, 3, 586, 593, Jun. 2007, [Peer-reviewed]
English, Scientific journal

Cell growth arrest by sialic acid clusters in ganglioside GM3 mimetic polymers
Satoshi Uemura, Fei Feng, Maya Kume, Kuriko Yamada, Kazuya Kabayama, Shin-Ichiro Nishimura, Yasuyuki Igarashi, Jin-Ichi Inokuchi
GLYCOBIOLOGY, 17, 6, 568, 577, Jun. 2007, [Peer-reviewed]
English, Scientific journal

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients
Hiroaki Nakagawa, Megumi Hato, Yasuhiro Takegawa, Kisaburo Deguchi, Hiroki Ito, Masahiko Takahata, Norimasa Iwasaki, Akio Minami, Shin-Ichiro Nishimura
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 853, 1-2, 133, 137, Jun. 2007, [Peer-reviewed]
English, Scientific journal

N-glycan alterations are associated with drug resistance in human hepatocellular carcinoma
Takeaki Kudo, Hiroaki Nakagawa, Masato Takahashi, Jun Hamaguchi, Naoya Kamiyama, Hideki Yokoo, Kazuaki Nakanishi, Takahito Nakagawa, Toshiya Kamiyama, Kisaburo Deguchi, Shin-Ichiro Nishimura, Satoru Todo
MOLECULAR CANCER, 6, 32, May 2007, [Peer-reviewed]
English, Scientific journal

Natural killer T cells from interleukin-4-deficient mice are defective in early interferon-gamma production in response to alpha-galactosylceramide
Yuji Togashi, Kenji Chamoto, Daiko Wakita, Naonobu Tsutsumi, Yoichiro Iwakura, Naoki Matsubara, Hidemitsu Kitamura, Takashi Nishimura
CANCER SCIENCE, 98, 5, 721, 725, May 2007, [Peer-reviewed]
English, Scientific journal

Molecular basis of guanine nucleotide dissociation inhibitor activity of human neuroglobin by chemical cross-linking and mass spectrometry
Chihiro Kitatsuji, Masaki Kurogochi, Shin-Ichiro Nishimura, Koichiro Ishimori, Keisuke Wakasugi
JOURNAL OF MOLECULAR BIOLOGY, 368, 1, 150, 160, Apr. 2007, [Peer-reviewed]
English, Scientific journal

The solution structure of horseshoe crab antimicrobial peptide tachystatin B with an inhibitory cystine-knot motif
Naoki Fujitani, Takahide Kouno, Taku Nakahara, Kenji Takaya, Tsukasa Osaki, Shun-Ichiro Kawabata, Mineyuki Mizuguchi, Tomoyasu Aizawa, Makoto Demura, Shin-Ichiro Nishimura, Keiichi Kawano
JOURNAL OF PEPTIDE SCIENCE, 13, 4, 269, 279, Apr. 2007, [Peer-reviewed]
English, Scientific journal

AsialoGM1(+)CD8(+) central memory-type T cells in unimmunized mice as novel immunomodulator of IFN-gamma-dependent type 1 immunity
Akemi Kosaka, Daiko Wakita, Naoki Matsubara, Yuji Togashi, Shin-Ichiro Nishimura, Hidemitsu Kitamura, Takashi Nishimura
INTERNATIONAL IMMUNOLOGY, 19, 3, 249, 256, Mar. 2007, [Peer-reviewed]
English, Scientific journal

A new glycosylation method. Part 2: Study of carbohydrate elongation onto the gold nanoparticles in a colloidal phase
Hiroki Shimizu, Masahiro Sakamoto, Noriko Nagahori, Shin-Ichiro Nishimura
Tetrahedron, 63, 11, 2418, 2425, Elsevier BV, Mar. 2007, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin
Hiroko Ito, Masashi Akiyama, Hiroaki Nakagawa, Rie Uematsu, Kisaburo Deguchi, James R. McMillan, Shin-Ichiro Nishimura, Hiroshi Shimizu
ARCHIVES OF DERMATOLOGICAL RESEARCH, 298, 8, 403, 407, Jan. 2007, [Peer-reviewed]
English, Scientific journal

Structural assignment of disialylated biantennary N-glycan isomers derivatized with 2-aminopyridine using negative-ion multistage tandem mass spectral matching
Hiroki Ito, Kuriko Yamada, Kisaburo Deguchi, Hiroaki Nakagawa, Shin-Ichiro Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21, 2, 212, 218, 2007, [Peer-reviewed]
English, Scientific journal

One-pot solid-phase glycoblotting and probing by transoximization for high-throughput glycomics and glycoproteomics
Hideyuki Shimaoka, Hiromitsu Kuramoto, Jun-ichi Furukawa, Yoshiaki Miura, Masaki Kurogochi, Yoko Kita, Hiroshi Hinou, Yasuro Shinohara, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 13, 6, 1664, 1673, 2007, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Structural analysis of O-glycopeptides employing negative- and positive-ion multi-stage mass spectra obtained by collision-induced and electron-capture dissociations in linear ion trap time-of-flight mass spectrometry
Kisaburo Deguchi, Hiroki Ito, Takashi Baba, Atsumu Hirabayashi, Hiroaki Nakagawa, Masataka Fumoto, Hiroshi Hinou, Shin-Ichiro Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21, 5, 691, 698, 2007, [Peer-reviewed]
English, Scientific journal

Highly efficient and versatile synthesis of proteoglycan core structures from 1,6-anhydro-beta-lactose as a key starting material
Ken Shimawaki, Yoshinori Fujisawa, Fumihiro Sato, Naoki Fujitani, Masaki Kurogochi, Hiroko Hoshi, Hiroshi Hinou, Shin-Ichiro Nishiinura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 46, 17, 3074, 3079, 2007, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Rapid and simple solid-phase esterification of sialic acid residues for quantitative glycomics by mass spectrometry
Yoshiaki Miura, Yasuro Shinohara, Jun-ichi Furukawa, Noriko Nagahori, Shin-ichiro Nishimurara
CHEMISTRY-A EUROPEAN JOURNAL, 13, 17, 4797, 4804, 2007, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers
Masaki Kurogochi, Maho Amano, Masataka Fumoto, Akio Takimoto, Hirosato Kondo, Shin-ichiro Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 46, 46, 8808, 8813, 2007, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycopolymers
S. I. Nishimura, N. Nagahori
Comprehensive Glycoscience: From Chemistry to Systems Biology, 3-4, 453, 476, Elsevier, 01 Jan. 2007
English, In book

Computational design and experimental evaluation of glycosyltransferase mutants: engineering of a blood type B galactosyltransferase with enhanced glucosyltransferase activity
Taku Nakahara, Ole Hindsgaul, Monica M. Palcic, Shin-Ichiro Nishimura
PROTEIN ENGINEERING DESIGN & SELECTION, 19, 12, 571, 578, Dec. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Construction and structural characterization of versatile lactosaminoglycan-related compound library for the synthesis of complex glycopeptides and glycosphingolipids
Kentarou Naruchi, Tomoki Hamamoto, Masaki Kurogochi, Hiroshi Hinou, Hiroki Shimizu, Takahiko Matsushita, Naoki Fujitani, Hirosato Kondo, Shin-Ichiro Nishimura
JOURNAL OF ORGANIC CHEMISTRY, 71, 26, 9609, 9621, Dec. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Rewinding of Helical Systems by Use of the Cr(III) Complex as a Photoresponsive Chiral Dopant
Tohru Taniguchi, Kenji Monde, Shin-Ichiro Nishimura, Jun Yoshida, Hisako Sato, Akihiko Yamagishi
Molecular Crystals and Liquid Crystals, 460, 1, 107, 116, Informa UK Limited, 01 Dec. 2006
Scientific journal

Unusual N-glycan structures in alpha-mannosidase II/IIx double null embryos identified by a systematic glycomics approach based on two-dimensional LC mapping and matrix-dependent selective fragmentation method in MALDI-TOF/TOF mass spectrometry
Megumi Hato, Hiroaki Nakagawa, Masaki Kurogochi, Tomoya O. Akama, Jamey D. Marth, Michiko N. Fukuda, Shin-Ichiro Nishimura
MOLECULAR & CELLULAR PROTEOMICS, 5, 11, 2146, 2157, Nov. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Interleukin-12-responding asialoGM1(+)CD8(+) central memory-type T cells as precursor cells for interferon-gamma-producing killer T cells
Akemi Kosaka, Ushaku Lee, Daiko Wakita, Naoki Matsubara, Yuji Togashi, Shin-Ichiro Nishimura, Hidemitsu Kitamura, Takashi Nishimura
CANCER SCIENCE, 97, 11, 1236, 1241, Nov. 2006, [Peer-reviewed]
English, Scientific journal

Specific cell behavior of human fibroblast onto carbohydrate surface detected by glycoblotting films
Tomohiro Onodera, Kenichi Niikura, Norimasa Iwasaki, Noriko Nagahori, Hideyuki Shimaoka, Ryusuke Kamitani, Tokifumi Majima, Akio Minami, Shin-Ichiro Nishimura
BIOMACROMOLECULES, 7, 11, 2949, 2955, Nov. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Simple separation of isomeric sialylated N-glycopeptides by a zwitterionic type of hydrophilic interaction chromatography
Yasuhiro Takegawa, Kisaburo Deguchi, Hiroki Ito, Takuro Keira, Hiroaki Nakagawa, Shin-Ichiro Nishimura
JOURNAL OF SEPARATION SCIENCE, 29, 16, 2533, 2540, Nov. 2006, [Peer-reviewed]
English, Scientific journal

Construction of MUC1 related compound library
Ohyabu Naoki, Matsushita Takahiko, Hinou Hiroshi, Izumi Ryuko, Shimizu Hiroki, Kondo Hirosato, Nishimura Shin-Ichiro
GLYCOBIOLOGY, 16, 11, 1152, 1152, Nov. 2006, [Peer-reviewed]
English

Structural analysis of O-glycopeptides employing negative- and positive-ion MSn spectra acquired by nanoHPLC/ESI-linear ion trap time-of-flight mass spectrometer
Ito Hiroki, Deguchi Kisaburo, Yamada Kuriko, Nagai Shinji, Fumoto Masataka, Hinou Hiroshi, Nakagawa Hiroaki, Shinohara Yasuro, Nishimura Shin-Ichiro
GLYCOBIOLOGY, 16, 11, 1136, Nov. 2006, [Peer-reviewed]

抗癌剤耐性ヒト肝癌細胞株におけるN-結合型糖鎖の構造変化　　　　　　　　　　　　　　　
工藤 岳秋, 中川 裕章, 高橋 將人, 神山 直也, 濱口 純, 横尾 英樹, 中西 一彰, 中川 隆公, 尾崎 倫孝, 神山 俊哉, 出口 喜三郎, 西村 紳一郎, 藤堂 省
日本癌学会総会記事, 65回, 100, 100, 日本癌学会, Sep. 2006
Japanese

Direct and efficient monitoring of glycosyltransferase reactions on gold colloidal nanoparticles by using mass spectrometry
Noriko Nagahori, Shin-Ichiro Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 12, 25, 6478, 6485, Aug. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Essential and mutually compensatory roles of alpha-mannosidase II and alpha-mannosidase IIx in N-glycan processing in vivo in mice
TO Akama, H Nakagawa, NK Wong, M Sutton-Smith, A Dell, HR Morris, J Nakayama, SI Nishimura, A Pai, KW Moremen, JD Marth, MN Fukuda
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103, 24, 8983, 8988, Jun. 2006, [Peer-reviewed]
English, Scientific journal

Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography
Y Takegawa, K Deguchi, T Keira, H Ito, H Nakagawa, S Nishimura
JOURNAL OF CHROMATOGRAPHY A, 1113, 1-2, 177, 181, Apr. 2006, [Peer-reviewed]
English, Scientific journal

N-acetylglucosaminyltransferase V and beta 1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer
H Ishimura, T Takahashi, H Nakagawa, SI Nishimura, Y Arai, Y Horikawa, T Habuchi, E Miyoshi, A Kyan, S Hagisawa, C Ohyama
CLINICAL CANCER RESEARCH, 12, 8, 2506, 2511, Apr. 2006, [Peer-reviewed]
English, Scientific journal

Construction of Highly Glycosylated Mucin-Type Glycopeptides Based on Microwave-Assisted Solid-Phase Syntheses and Enzymatic Modifications
Takahiko Matsushita, Hiroshi Hinou, Masataka Fumoto, Masaki Kurogochi, Naoki Fujitani, Hiroki Shimizu, Shin-Ichiro Nishimura
The Journal of Organic Chemistry, 71, 8, 3051, 3063, American Chemical Society (ACS), Apr. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography
Yasuhiro Takegawa, Kisaburo Deguchi, Takuro Keira, Hiroki Ito, Hiroaki Nakagawa, Shin-Ichiro Nishimura
Journal of Chromatography A, 1113, 1-2, 177, 181, Elsevier BV, Apr. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Effects of synthetic antifreeze glycoprotein analogue on islet cell survival and function during cryopreservation
S Matsumoto, M Matsusita, T Morita, H Kamachi, S Tsukiyama, Y Furukawa, S Koshida, Y Tachibana, S Nishimura, S Todo
CRYOBIOLOGY, 52, 1, 90, 98, Feb. 2006, [Peer-reviewed]
English, Scientific journal

A theoretical study of α- and β-d-glucopyranose conformations by the density functional theory
Nobuaki Miura, Tohru Taniguchi, Kenji Monde, Shin-Ichiro Nishimura
Chemical Physics Letters, 419, 4-6, 326, 332, Elsevier BV, Feb. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Structural assignment of isomeric 2-aminopyridine-derivatized monosialylated biantennary N-linked oligosaccharides using negative-ion multistage tandem mass spectral matching
K Deguchi, Y Takegawa, H Ito, N Miura, S Yoshioka, S Nagai, H Nakagawa, SI Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 20, 3, 412, 418, 2006, [Peer-reviewed]
English, Scientific journal

Complementary structural information of positive- and negative-ion MSn spectra of glycopeptides with neutral and sialylated N-glycans
K Deguchi, H Ito, Y Takegawa, N Shinji, H Nakagawa, SI Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 20, 5, 741, 746, 2006, [Peer-reviewed]
English, Scientific journal

Glycosidation promoted by a reusable solid superacid in supercritical carbon dioxide
Xue-Bing Li, Masato Ogawa, Toshiki Monden, Takahiro Maeda, Eri Yamashita, Mariko Naka, Masao Matsuda, Hiroshi Hinou, Shin-Ichiro Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 45, 34, 5652, 5655, 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Direct structural assignment of neutral and sialylated N-glycans of glycopeptides using collision-induced dissociation MSn spectral matching
Hiroki Ito, Yasuhiro Takegawa, Kisaburo Deguchi, Shinji Nagai, Hiroaki Nakagawa, Yasuro Shinohara, Shin-Ichiro Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 20, 23, 3557, 3565, 2006, [Peer-reviewed]
English, Scientific journal

Mechanism-based inhibitors to probe transitional states of glycoside hydrolases
Hiroshi Hinou, Masaki Kurogochi, Shin-Ichiro Nishimura
GLYCOBIOLOGY, 415, 202, +, 2006, [Peer-reviewed], [Last author, Corresponding author]
English, In book

Controlled synthesis of artificial glycoprotein using cell-free system　　　　　　　　　　　　　　　
Reiko Sadamoto, Kei Oiwa, Naoki Matsubara, Takeshi Matsubayashi, Norio Fukuhara, Hiroaki Suga, Shin-Ichiro Nishimura
Polymer Preprints, Japan, 55, 2, 5274, 5275, 2006
Japanese, International conference proceedings

High density sugar-displaying materials using the glycoblotting methods for cell patterning　　　　　　　　　　　　　　　
Ryosuke Kamitani, Kenichi Niikura, Tomohiro Onodera, Norimasa Iwasaki, Masataka Fumoto, Hideyuki Shimaoka, Kuniharu Ijiro, Shin-Ichiro Nishimura
Polymer Preprints, Japan, 55, 1, 1895, 2006
Japanese, International conference proceedings

High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis
R Uematsu, J Furukawa, H Nakagawa, Y Shinohara, K Deguchi, K Monde, SI Nishimura
MOLECULAR & CELLULAR PROTEOMICS, 4, 12, 1977, 1989, Dec. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Molecular design of glycoprotein mimetics: Glycoblotting by engineered proteins with an oxylamino-functionalized amino acid residue
N Matsubara, K Oiwa, T Hohsaka, R Sadamoto, K Niikura, N Fukuhara, A Takimoto, H Kondo, S Nishimurar
CHEMISTRY-A EUROPEAN JOURNAL, 11, 23, 6974, 6981, Nov. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Chitosan-RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering
T Masuko, N Iwasaki, S Yamane, T Funakoshi, T Majima, A Minami, N Ohsuga, T Ohta, SI Nishimura
BIOMATERIALS, 26, 26, 5339, 5347, Sep. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Characterization of Vibrio cholerae neuraminidase by a novel mechanism-based fluorescent labeling reagent
H Hinou, M Kurogochi, H Shimizu, SI Nishimura
BIOCHEMISTRY, 44, 35, 11669, 11675, Sep. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Structural analysis of an N-glycan with "beta 1-4 bisecting branch" from human serum IgG by negative-ion MSn spectral matching and exoglycosidase digestion
Y Takegawa, K Deguchi, H Nakagawa, SI Nishimura
ANALYTICAL CHEMISTRY, 77, 18, 6062, 6068, Sep. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Rational design, synthesis, and characterization of novel inhibitors for human beta 1,4-galactosyltransferase
K Takaya, N Nagahori, M Kurogochi, T Furuike, N Miura, K Monde, YC Lee, SI Nishimura
JOURNAL OF MEDICINAL CHEMISTRY, 48, 19, 6054, 6065, Sep. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Novel chitosan-based hyaluronan hybrid polymer fibers as a scaffold in ligament tissue engineering.
Tadanao Funakoshi, Tokifumi Majima, Norimasa Iwasaki, Shintaro Yamane, Tatsuya Masuko, Akio Minami, Kazuo Harada, Hiroshi Tamura, Seiichi Tokura, Shin-Ichiro Nishimura
Journal of biomedical materials research. Part A, 74, 3, 338, 46, 01 Sep. 2005, [International Magazine]
English, Scientific journal, To clarify the feasibility of using novel chitosan-based hyaluronan hybrid polymer fibers as a scaffold in ligament tissue engineering, their mechanical properties and ability to promote cellular adhesion, proliferation, and extracellular matrix production were studied in vitro. Chitosan fibers and chitosan-based 0.05% and 0.1% hyaluronan hybrid fibers were developed by the wet spinning method. Hyaluronan coating significantly increased mechanical properties, compared to the chitosan fibers. Rabbit fibroblasts adhesion onto hybrid fibers was significantly greater than for the control and chitosan fibers. For analysis of cell proliferation and extracellular matrix production, a three-dimensional scaffold was created by simply piling up each fiber. At 1 day after cultivation, the DNA content in the hybrid scaffolds was higher than that in the chitosan scaffold. Scanning electron microscopy showed that the fibroblasts had produced collagen fibers after 14 days of culture. Immunostaining for type I collagen was clearly predominant in the hybrid scaffolds, and the mRNA level of type I collagen in the hybrid scaffolds were significantly greater than that in the chitosan scaffold. The present study revealed that hyaluronan hybridization with chitosan fibers enhanced fiber mechanical properties and in vitro biological effects on the cultured fibroblasts.

Novel chitosan-based hyaluronan hybrid polymer fibers as a scaffold in ligament tissue engineering
Tadanao Funakoshi, Tokifumi Majima, Norimasa Iwasaki, Shintaro Yamane, Tatsuya Masuko, Akio Minami, Kazuo Harada, Hiroshi Tamura, Seiichi Tokura, Shin-Ichiro Nishimura
Journal of Biomedical Materials Research - Part A, 74, 3, 338, 346, 01 Sep. 2005, [Peer-reviewed]
English, Scientific journal

Application of tissue engineering techniques for rotator cuff regeneration using a chitosan-based hyaluronan hybrid fiber scaffold
T Funakoshi, T Majima, N Iwasaki, N Suenaga, N Sawaguchi, K Shimode, A Minami, K Harada, S Nishimura
AMERICAN JOURNAL OF SPORTS MEDICINE, 33, 8, 1193, 1201, Aug. 2005, [Peer-reviewed]
English, Scientific journal

Combinatorial synthesis of MUC1 glycopeptides: Polymer blotting facilitates chemical and enzymatic synthesis of highly complicated mucin glycopeptides
M Fumoto, H Hinou, T Ohta, T Ito, K Yamada, A Takimoto, H Kondo, H Shimizu, T Inazu, Y Nakahara, SI Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 127, 33, 11804, 11818, Aug. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

A novel glycosylation concept; microwave-assisted acetal-exchange type glycosylations from methyl glycosides as donors
Yayoi Yoshimura, Hiroki Shimizu, Hiroshi Hinou, Shin-Ichiro Nishimura
Tetrahedron Letters, 46, 28, 4701, 4705, Elsevier BV, Jul. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Versatile glycoblotting nanoparticles for high-throughput protein glycomics
K Niikura, R Kamitani, M Kurogochi, R Uematsu, Y Shinohara, H Nakagawa, K Deguchi, K Monde, H Kondo, SI Nishimura
CHEMISTRY-A EUROPEAN JOURNAL, 11, 13, 3825, 3834, Jun. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Alginate and chitosan polyion complex hybrid fibers for scaffolds in ligament and tendon tissue engineering
T Majima, T Funakosi, N Iwasaki, ST Yamane, K Harada, S Nonaka, A Minami, SI Nishimura
JOURNAL OF ORTHOPAEDIC SCIENCE, 10, 3, 302, 307, May 2005, [Peer-reviewed]
English, Scientific journal

Carbohydrate analysis by a phenol-sulfuric acid method in microplate format
T Masuko, A Minami, N Iwasaki, T Majima, SI Nishimura, YC Lee
ANALYTICAL BIOCHEMISTRY, 339, 1, 69, 72, Apr. 2005, [Peer-reviewed]
English, Scientific journal

Simultaneous analysis of 2-aminopyridine-derivatized neutral and sialylated oligosaccharides from human serum in the negative-ion mode by sonic spray ionization ion trap mass spectrometry
Y Takegawa, K Deguchi, S Ito, S Yoshioka, H Nakagawa, S Nishimura
ANALYTICAL CHEMISTRY, 77, 7, 2097, 2106, Apr. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Organic Matter Released from Activated Sludge Bacteria Cells During Their Decay Process
H. Narita, I. Isshiki, N. Funamizu, T. Takakuwa, H. Nakagawa, S-I. Nishimura
Environmental Technology, 26, 4, 433, 440, Informa UK Limited, Apr. 2005, [Peer-reviewed]
English, Scientific journal

Rapid microwave-assisted solid-phase glycopeptide synthesis
T Matsushita, H Hinou, M Kurogochi, H Shimizu, SI Nishimura
ORGANIC LETTERS, 7, 5, 877, 880, Mar. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Thiolation of chitosan. Attachment of proteins via thioether formation
T Masuko, A Minami, N Iwasaki, T Majima, SI Nishimura, YC Lee
BIOMACROMOLECULES, 6, 2, 880, 884, Mar. 2005, [Peer-reviewed]
English, Scientific journal

Feasibility of chitosan-based hyaluronic acid hybrid biomaterial for a novel scaffold in cartilage tissue engineering
S Yamane, N Iwasaki, T Majima, T Funakoshi, T Masuko, K Harada, A Minami, K Monde, S Nishimura
BIOMATERIALS, 26, 6, 611, 619, Feb. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Chemical and enzymatic synthesis of glycocluster having seven sialyl lewis X arrays using β-cyclodextrin as a key scaffold material
Tetsuya Furuike, Reiko Sadamoto, Kenichi Niikura, Kenji Monde, Nobuo Sakairi, Shin-Ichiro Nishimura
Tetrahedron, 61, 7, 1737, 1742, Elsevier BV, Feb. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Direct observation of sugar-protein, sugar-sugar, and sugar-water complexes by cold-spray ionization time-of-flight mass spectrometry
SI Nishimura, N Nagahori, K Takaya, Y Tachibana, N Miura, K Monde
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 44, 4, 571, 575, 2005, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

High-throughput protein glycomics: Combined use of chemoselective glycoblotting and MALDI-TOF/TOF mass spectrometry
SI Nishimura, K Niikura, M Kurogochi, T Matsushita, M Fumoto, H Hinou, R Kamitani, H Nakagawa, K Deguchi, N Miura, K Monde, H Kondo
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 44, 1, 91, 96, 2005, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using negative-ion MSn spectral matching
Y Takegawa, K Deguchi, S Ito, S Yoshioka, H Nakagawa, SI Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 19, 7, 937, 946, 2005, [Peer-reviewed]
English, Scientific journal

Molecular transporter between polymer platforms: Highly efficient chemoenzymatic glycopeptide synthesis by the combined use of solid-phase and water-soluble polymer supports
M Fumoto, H Hinou, T Matsushita, M Kurogochi, T Ohta, T Ito, K Yamada, A Takimoto, H Kondo, T Inazu, SI Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 44, 17, 2534, 2537, 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Nanometer-scale ordering in cast films of columnar metallomesogen as revealed by STM observations
N Kakegawa, N Hoshino, Y Matsuoka, N Wakabayashi, SI Nishimura, A Yamagishi
CHEMICAL COMMUNICATIONS, 18, 2375, 2377, 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Characteristic pH and electrolyte concentration dependences of 2-aminopyridine-derivatized oligosaccharides (N-glycans) in sonic-spray ionization mass spectrometry
K Deguchi, Y Takegawa, A Hirabayashi, H Nakagawa, SI Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 19, 16, 2325, 2330, 2005, [Peer-reviewed]
English, Scientific journal

Chemo-biological approach to modification of the bacterial cell wall　　　　　　　　　　　　　　　
Reiko Sadamoto, Shin-Ichiro Nishimura
Handbook of Carbohydrate Engineering, 495, 506, CRC Press, 01 Jan. 2005
English, In book

The elucidation of a proteoglycan biosynthesis mechanism by organic synthesis　　　　　　　　　　　　　　　
Ken Shimawaki, Hiroko Hoshi, Yoshinori Fujisawa, Kenji Monde, Shin-Ichiro Nishimura
Polymer Preprints, Japan, 54, 1, 2155, 2005
Japanese, International conference proceedings

Antifreeze Glycoprotein: Elucidation of Antifreeze Mechanism　　　　　　　　　　　　　　　
Masakazu Hachisu, Kohta Igarashi, Sakae Tsuda, Shuhei Koshida, Kenji Monde, Shin-Ichiro Nishimura
Polymer Preprints, Japan, 54, 1, 2168, 2005
Japanese, International conference proceedings

Versatile synthesis of oligosaccharide-containing fullerenes
Shigeaki Abe, Hideki Moriyama, Kenichi Niikura, Fei Feng, Kenji Monde, Shin-Ichiro Nishimura
Tetrahedron: Asymmetry, 16, 1, 15, 19, Elsevier BV, Jan. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Direct N-glycan profiling in the presence of tryptic peptides on MALDI-TOF by controlled ion enhancement and suppression upon glycan-selective derivatization
Y Shinohara, J Furukawa, K Niikura, N Miura, SI Nishimura
ANALYTICAL CHEMISTRY, 76, 23, 6989, 6997, Dec. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Assignment and quantification of 2-aminopyridine derivatized oligosaccharide isomers coeluted on reversed-phase HPLC/MS by MSn spectral library
Y Takegawa, K Deguchi, S Ito, S Yoshioka, A Sano, K Yoshinari, K Kobayashi, H Nakagawa, K Monde, SI Nishimura
ANALYTICAL CHEMISTRY, 76, 24, 7294, 7303, Dec. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycoinsulins: Dendritic sialyloligosaccharide-displaying insulins showing a prolonged blood-sugar-lowering activity
M Sato, T Furuike, R Sadamoto, N Fujitani, T Nakahara, K Niikura, K Monde, H Kondo, SI Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 126, 43, 14013, 14022, Nov. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

A characteristic CH band in VCD of methyl glycosidic carbohydrates
Tohru Taniguchi, Kenji Monde, Nobuaki Miura, Shin-Ichiro Nishimura
Tetrahedron Letters, 45, 46, 8451, 8453, Elsevier BV, Nov. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Mechanism-based fluorescent labeling of beta-galactosidases - An efficient method in proteomics for glycoside hydrolases
M Kurogochi, SI Nishimura, YC Lee
JOURNAL OF BIOLOGICAL CHEMISTRY, 279, 43, 44704, 44712, Oct. 2004, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Structural characterization of N-glycopeptides by matrix-dependent selective fragmentation of MALDI-TOF/TOF tandem mass spectrometry
M Kurogochi, SI Nishimura
ANALYTICAL CHEMISTRY, 76, 20, 6097, 6101, Oct. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

71(P-61) Stereochemical Studies of Glycoconjugates by Vibrational Circular Dichroism (VCD)
Taniguchi Tohru, Miura Nobuaki, Uchida Naoto, Nishimura Shin-Ichiro, Monde Kenji
Symposium on the Chemistry of Natural Products, symposium papers, 46, 401, 406, Symposium on the chemistry of natural products, 01 Oct. 2004
Japanese, Chiral molecules exhibit circular dichroism (CD), the differential absorption of left versus right circularly polarized light, and the spectroscopies have been useful method for determining the absolute configuration. Recently, the measurement of CD in an infrared region, i.e. vibrational circular dichroism (VCD), has been advanced instrumentally and theoretically. VCD has some advantages over conventional electronic circular dichroism (ECD) by the applicability to all organic molecules and the reliability of ab initio quantum calculations. We apply this powerful technique to the determination of the absolute stereochemistry of some cruciferous phytoalexin-related compounds and to analysis of glycoconjugates. The determination of the absolute configuration of cruciferous phytoalexin-related compounds A number of cruciferous phytoalexin studies have been reported so far, but most of their absolute stereochemistry is still to be investigated. So we applied the VCD spectroscopy to determine the absolute configuration of dioxibrassinin (1) and 3-cyanomethyl-3-hydroxyoxindole (2). Compounds 1 and 2 were synthesized and their VCD spectra were measured. The comparison of observed VCD spectra and calculated ones indicated that their stereochemistry are both S. This is the first case of the determination of absolute stereochemistry of chiral tertiary alcohols by VCD. Application of VCD to carbohydrates Recently, the biological significance of carbohydrates have been recognized and analytical studies of carbohydrates have been widely investigated. Most analyses have been based on achiral methods such as NMR or MS in spite of their chiral properties. We considered that the detailed stereochemical information could be extracted using VCD. Various carbohydrates have been measured to construct an extensive database aiming to create a methodology for VCD pattern discrimination of the carbohydrates. The database in the fingerprint region indicated that sugars with the axial glycosidic linkage showed a negative, sharp and intense VCD band, named "Glycoside band" at around 1145 cm^<-1>. The applicability of the Glycoside band was demonstrated by quantifying α-β ratio of mixture samples and monitoring of an enzymatic reaction. In the C-H stretching region, we found the stereochemistry of single chiral center in the presence of multiple such centers could be extracted through the vibrational chirality transfer phenomenon. Also, VCD in this region was indicated to be a sensitive probe of glycosidic linkage sites. In conclusion, by using the VCD database and the proposed spectra-structure correlations, VCD will be an effective tool in carbohydrate analysis.

Vibrational circular dichroism: Chiroptical analysis of biomolecules
T Taniguchi, N Miura, SI Nishimura, K Monde
MOLECULAR NUTRITION & FOOD RESEARCH, 48, 4, 246, 254, Sep. 2004, [Peer-reviewed]
English, Scientific journal

Sequential Glycoproteins:  Practical Method for the Synthesis of Antifreeze Glycoprotein Models Containing Base Labile Groups
Yuki Tachibana, Kenji Monde, Shin-Ichiro Nishimura
Macromolecules, 37, 18, 6771, 6779, American Chemical Society (ACS), Sep. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Specific Band Observed in VCD Predicts the Anomeric Configuration of Carbohydrates
Kenji Monde, Tohru Taniguchi, Nobuaki Miura, Shin-Ichiro Nishimura
Journal of the American Chemical Society, 126, 31, 9496, 9497, American Chemical Society (ACS), Aug. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Feasibility of polysaccharide hybrid materials for scaffolds in cartilage tissue engineering: Evaluation of chondrocyte adhesion to polyion complex fibers prepared from alginate and chitosan
N Iwasaki, ST Yamane, T Majima, Y Kasahara, A Minami, K Harada, S Nonaka, N Maekawa, H Tamura, S Tokura, M Shiono, K Monde, SI Nishimura
BIOMACROMOLECULES, 5, 3, 828, 833, May 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Synthesis of 4-fluorinated UDP-MurNAc pentapeptide as an inhibitor of bacterial growth
T Ueda, F Feng, R Sadamoto, K Niikura, K Monde, SI Nishimura
ORGANIC LETTERS, 6, 11, 1753, 1756, May 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Azasugar-based MMP/ADAM inhibitors as antipsoriatic agents
H Moriyama, T Tsukida, Y Inoue, K Yokota, K Yoshino, H Kondo, N Miura, S Nishimura
JOURNAL OF MEDICINAL CHEMISTRY, 47, 8, 1930, 1938, Apr. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Generation of leukemia-specific T-helper type 1 cells applicable to human leukemia cell-therapy
T Fujimura, K Chamoto, T Tsuji, T Sato, H Yokouchi, S Aiba, H Tagami, J Tanaka, M Imamura, Y Togashi, T Koda, T Nishimura
IMMUNOLOGY LETTERS, 93, 1, 17, 25, Apr. 2004, [Peer-reviewed]
English, Scientific journal

A practical synthesis of amphiphilic cyclodextrins fully substituted with sugar residues on the primary face
F Sallas, K Niikura, SI Nishimura
CHEMICAL COMMUNICATIONS, 5, 596, 597, Mar. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Synthesis and biological activity of selective azasugar-based TACE inhibitors
T Tsukida, H Moriyama, Y Inoue, H Kondo, K Yoshino, SI Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 14, 6, 1569, 1572, Mar. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Control of bacteria adhesion by cell-wall engineering
R Sadamoto, K Niikura, T Ueda, K Monde, N Fukuhara, SI Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 126, 12, 3755, 3761, Mar. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Fluorescent Glyconanoparticles as a Sensitive Device to Monitor Sugar-Involving Molecular Events
Kenichi Niikura, Noriko Osuga, Noriko Nagahori, Reiko Sadamoto, Masamichi Shiono, Norimasa Iwasaki, Kenji Monde, Akio Minami, Shin-Ichiro Nishimura
Polymer Journal, 36, 3, 209, 218, Springer Science and Business Media LLC, Mar. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

IFN-gamma-induced SOCS-1 regulates STAT6-dependent eotaxin production triggered by IL-4 and TNF-alpha
T Sato, R Saito, T Jinushi, T Tsuji, J Matsuzaki, T Koda, S Nishimura, H Takeshima, T Nishimura
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 314, 2, 468, 475, Feb. 2004, [Peer-reviewed]
English, Scientific journal

An engineered hyaluronan synthase - Characterization of recombinant human hyaluronan synthase 2 expressed in Escherichia coli
H Hoshi, H Nakagawa, S Nishiguchi, K Iwata, K Niikura, K Monde, SI Nishimura
JOURNAL OF BIOLOGICAL CHEMISTRY, 279, 4, 2341, 2349, Jan. 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Persubstituted cyclodextrin-based glycoclusters as inhibitors of protein-carbohydrate recognition using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targets
S Andre, H Kaltner, T Furuike, SI Nishimura, HJ Gabius
BIOCONJUGATE CHEMISTRY, 15, 1, 87, 98, Jan. 2004, [Peer-reviewed]
English, Scientific journal

Antifreeze glycoproteins: Elucidation of the structural motifs that are essential for antifreeze activity
Y Tachibana, GL Fletcher, N Fujitani, S Tsuda, K Monde, SI Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 43, 7, 856, 862, 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using MSn spectral matching
Y Takegawa, S Ito, S Yoshioka, K Deguchi, H Nakagawa, K Monde, SI Nishimura
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 18, 4, 385, 391, 2004, [Peer-reviewed]
English, Scientific journal

Site-specific introduction of sialic acid into insulin
M Sato, R Sadamoto, K Niikura, K Monde, H Kondo, SI Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 43, 12, 1516, 1520, 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Chemo-enzymatic synthesis of fluorinated 2-N-acetamidosugar nucleotides using UDP-GlcNAc pyrophosphorylase
F Feng, K Okuyama, K Niikura, T Ohta, R Sadamoto, K Monde, T Noguchi, SI Nishimura
ORGANIC & BIOMOLECULAR CHEMISTRY, 2, 11, 1617, 1623, 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Post-translational modifications on proteins: Facile and efficient procedure for the identification of O-glycosylation sites by MALDI-LIFT-TOF/TOF mass spectrometry
M Kurogochi, T Matsushita, SI Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 43, 31, 4071, 4075, 2004, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Identification of Th1- and Th2-speciric genes by microarray analysis
J Li, T Koda, A Yamaguchi, S Yamamoto, T Sato, Y Togashi, T Nishimura
BIOMEDICAL RESEARCH-TOKYO, 24, 6, 299, 307, Dec. 2003, [Peer-reviewed]
English, Scientific journal

Functional expression of the TrkC gene, encoding a high affinity receptor for NT-3, in antigen-specific T helper type 2 (Th2) cells
Masashi Sekimoto, Takemasa Tsuji, Jyunko Matsuzaki, Kenji Chamoto, Toshiaki Koda, Kiyomitsu Nemoto, Masakuni Degawa, Shin-ichiro Nishimura, Takashi Nishimura
Immunology Letters, 88, 3, 221, 226, Elsevier BV, Sep. 2003, [Peer-reviewed]
English, Scientific journal

Structure-activity relationships of azasugar-based MMP/ADAM inhibitors
H Moriyama, T Tsukida, Y Inoue, H Kondo, K Yoshino, SI Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 13, 16, 2737, 2740, Aug. 2003, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Design, synthesis and evaluation of novel azasugar-based MMP/ADAM inhibitors
H Moriyama, T Tsukida, Y Inoue, H Kondo, K Yoshino, SI Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 13, 16, 2741, 2744, Aug. 2003, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Preparation of cruciferous phytoalexin related metabolites, (−)-dioxibrassinin and (−)-3-cyanomethyl-3-hydroxyoxindole, and determination of their absolute configurations by vibrational circular dichroism (VCD)
Kenji Monde, Tohru Taniguchi, Nobuaki Miura, Shin-Ichiro Nishimura, Nobuyuki Harada, Rina K Dukor, Laurence A Nafie
Tetrahedron Letters, 44, 32, 6017, 6020, Elsevier BV, Aug. 2003, [Peer-reviewed]
English, Scientific journal

Differential regulation of VLA-2 expression on T(h)1 and T(h)2 cells: a novel marker for the classification of T-h subsets (vol 15, pg 701, 2003)
K Sasaki, T Tsuji, T Jinushi, J Matsuzaki, T Sato, K Chamoto, Y Togashi, T Koda, T Nishimura
INTERNATIONAL IMMUNOLOGY, 15, 7, 893, 893, Jul. 2003, [Peer-reviewed]
English

An efficient synthesis of a biantennary sialooligosaccharide analog using a 1,6-anhydro-β-lactose derivative as a key synthetic block
Tetsuya Furuike, Kuriko Yamada, Takashi Ohta, Kenji Monde, Shin-Ichiro Nishimura
Tetrahedron, 59, 27, 5105, 5113, Elsevier BV, Jun. 2003, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Glycosyltransferase Microarray Displayed on the Glycolipid LB Membrane
Noriko Nagahori, Kenichi Niikura, Reiko Sadamoto, Masahiro Taniguchi, Akihiko Yamagishi, Kenji Monde, Shin-Ichiro Nishimura
Advanced Synthesis & Catalysis, 345, 67, 729, 734, Wiley, Jun. 2003, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Synthesis of a useful anomeric thioacetate of an N-acetyllactosamine derivative and its application
Koji Matsuoka, Takumi Ohtawa, Hiroshi Hinou, Tetsuo Koyama, Yasuaki Esumi, Shin-Ichiro Nishimura, Ken Hatano, Daiyo Terunuma
Tetrahedron Letters, 44, 18, 3617, 3620, Elsevier BV, Apr. 2003, [Peer-reviewed]
English, Scientific journal

Design, synthesis, and evaluation of β-galactosylceramide mimics promoting β-glucocerebrosidase activity in keratinocytes
Kyoko Fukunaga, Masahiro Yoshida, Fumio Nakajima, Rie Uematsu, Mariko Hara, Shintaro Inoue, Hirosato Kondo, Shin-Ichiro Nishimura
Bioorganic & Medicinal Chemistry Letters, 13, 5, 813, 815, Elsevier BV, Mar. 2003, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Detection of genes with tissue-specific expression patterns using Akaike's information criterion procedure.
Kadota K, Nishimura S, Bono H, Nakamura S, Hayashizaki Y, Okazaki Y, Takahashi K
Physiological genomics, 12, 3, 251, 259, Feb. 2003, [Peer-reviewed]

Structure determination and conformational change induced by tyrosine phosphorylation of the N-terminal domain of the alpha-chain of pig gastric H+/K+-ATPase
N Fujitani, M Kanagawa, T Aizawa, T Ohkubo, S Kaya, M Demura, K Kawano, S Nishimura, K Taniguchi, K Nitta
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 300, 1, 223, 229, Jan. 2003, [Peer-reviewed]
English, Scientific journal

Glycotentacles: Synthesis of cyclic glycopeptides, toward a tailored blocker of influenza virus hemagglutinin
T Ohta, N Miura, N Fujitani, F Nakajima, K Niikura, R Sadamoto, CT Guo, T Suzuki, Y Suzuki, K Monde, SI Nishimura
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 42, 42, 5186, 5189, 2003, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Protective action of reduced water against alloxan-induced cytotoxicity in pancreatic beta-cell
YP Li, T Nishimura, K Teruya, Y Katakura, S Kabayama, K Otsubo, S Morisawa, Y Ishii, Z Gadek, S Shirahata
ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 13, 145, 149, 2003, [Peer-reviewed]
English, International conference proceedings

Cell wall engineering of living bacteria through biosynthesis.
Sadamoto R, Niikura K, Monde K, Nishimura S
Methods in enzymology, 362, 273, 286, 2003, [Peer-reviewed]

Synthesis of Photopolymerizable Glycolipids and their Application as Scaffolds to Immobilize Proteins with a Micron-Sized Pattern
Noriko Nagahori, Kenichi Niikura, Reiko Sadamoto, Kenji Monde, Shin-Ichiro Nishimura
Australian Journal of Chemistry, 56, 6, 567, 567, CSIRO Publishing, 2003, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, Photopolymerizable glycolipids incorporating ceramide- or amido-type linkers and able to form stable monolayers were efficiently synthesized by chemical and enzymatic methods. Glycolipid polymer films served as platforms for the immobilization of proteins through specific carbohydrate–protein interactions at the air–water interface. Carbohydrate-binding proteins deposited on the glycolipid film were observed by atomic force microscopy, which showed varying submicron-sized protein patterns such as dendrites, dots, and networks, depending on the lipid structure, membrane preparation process, and sugar density of the membrane. Surface plasmon resonance measurement confirmed that the subunit-type lectins immobilized on the glycolipid membranes exhibited the ability to interact specifically with carbohydrate ligands by using unoccupied binding sites.

Efficient and versatile synthesis of mucin-like glycoprotein mimics
Yuki Tachibana, Naoki Matsubara, Fumio Nakajima, Tetsuro Tsuda, Sakae Tsuda, Kenji Monde, Shin-Ichiro Nishimura
Tetrahedron, 58, 51, 10213, 10224, Elsevier BV, Dec. 2002, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Inhibition of adhesion of type 1 fimbriated Escherichia coli to highly mannosylated ligands
N Nagahori, RT Lee, S Nishimura, D Page, R Roy, YC Lee
CHEMBIOCHEM, 3, 9, 836, 844, Sep. 2002, [Peer-reviewed]
English, Scientific journal

Cell-wall engineering of living bacteria
R Sadamoto, K Niikura, PS Sears, HT Liu, CH Wong, A Suksomcheep, F Tomita, K Monde, SI Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 124, 31, 9018, 9019, Aug. 2002, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Preparation of Self-Organized Micro-Patterned Polymer Films Having Cell Adhesive Ligands
Jin Nishida, Kazutaka Nishikawa, Shin-Ichiro Nishimura, Shigeo Wada, Takeshi Karino, Takehiro Nishikawa, Kuniharu Ijiro, Masatsugu Shimomura
Polymer Journal, 34, 3, 166, 174, Springer Science and Business Media LLC, Mar. 2002, [Peer-reviewed]
English, Scientific journal

Encounter with unexpected collagenase-1 selective inhibitor: Switchover of inhibitor binding pocket induced by fluorine atom
M Sawa, H Kondo, S Nishimura
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 12, 4, 581, 584, Feb. 2002, [Peer-reviewed]
English, Scientific journal

Germ cell survival through carbohydrate-mediated interaction with Sertoli cells
Tomoya O. Akama, Hiroaki Nakagawa, Kazuhiro Sugihara, Sonoko Narisawa, Chikara Ohyama, Shin Ichiro Nishimura, Deborah A. O'Brien, Kelley W. Moremen, José Luis Millán, Michiko N. Fukuda
Science, 295, 5552, 124, 127, 5552, 04 Jan. 2002, [Peer-reviewed]
Scientific journal

Erythrocyte-like liposomes prepared by means of amphiphilic cyclodextrin sulfates
T Sukegawa, T Furuike, K Niikura, A Yamagishi, K Monde, SI Nishimura
CHEMICAL COMMUNICATIONS, 5, 430, 431, 2002, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

An Engineered Biocatalyst for the Synthesis of Glycoconjugates: Utilization of β1,3-N-Acetyl-D-glucosaminyltransferase from Streptococcus agalactiae Type Ia Expressed in Escherichia coli as a Fusion with Maltose-Binding Protein
Atsushi Toda, Kuriko Yamada, Shin-Ichiro Nishimura
Advanced Synthesis & Catalysis, 344, 1, 61, 61, Wiley, Jan. 2002, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Th1 cytokine-conditioned bone marrow-derived dendritic cells can bypass the requirement for Th functions during the generation of CD8(+) CTL
M Sato, K Chamoto, T Tsuji, Y Iwakura, Y Togashi, T Koda, T Nishimura
JOURNAL OF IMMUNOLOGY, 167, 7, 3687, 3691, Oct. 2001, [Peer-reviewed]
English, Scientific journal

Characterization of a human H9N2 influenza virus isolated in Hong Kong
Takehiko Saito, Wilina Lim, Takashi Suzuki, Yasuo Suzuki, Hiroshi Kida, Shin-Ichiro Nishimura, Masato Tashiro
Vaccine, 20, 1-2, 125, 133, Elsevier BV, Oct. 2001, [Peer-reviewed]
English, Scientific journal

Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports
S Nishiguchi, K Yamada, Y Fuji, S Shibatani, A Toda, SI Nishimura, SI Nishimura
CHEMICAL COMMUNICATIONS, 19, 1944, 1945, Oct. 2001, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Combinatorial syntheses of sugar derivatives
Shin-Ichiro Nishimura
Current Opinion in Chemical Biology, 5, 3, 325, 335, Elsevier BV, Jun. 2001, [Peer-reviewed], [Lead author, Last author, Corresponding author]
English, Scientific journal

Cyclic di-t-butylsilylenediyl ether group as a convenient protective group for the glycoconjugate synthesis
Daijyu Kumagai, Masaki Miyazaki, Shin-Ichiro Nishimura
Tetrahedron Letters, 42, 10, 1953, 1956, 04 Mar. 2001, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Heterobifunctional Ligands:  Practical Chemoenzymatic Synthesis of a Cell Adhesive Glycopeptide That Interacts with Both Selectins and Integrins
Masao Matsuda, Shin-Ichiro Nishimura, Fumio Nakajima, Takashi Nishimura
Journal of Medicinal Chemistry, 44, 5, 715, 724, American Chemical Society (ACS), Mar. 2001, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Anti-oxidative water improves diabetes
S Shirahata, T Nishimura, S Kabayama, D Aki, K Teruya, K Otsubo, S Morisawa, Y Ishii, Z Gadek, Y Katakura
ANIMAL CELL TECHNOLOGY: FROM TARGET TO MARKET, 1, 574, 577, 2001, [Peer-reviewed]
English, International conference proceedings

Human N-Acetylglucosaminyltransferase I. Expression in Escherichia coli as a soluble enzyme, and application as an immobilized enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides
Kazuhito Fujiyama, Yoshihiro Ido, Ryo Misaki, Daniel G. Moran, Itaru Yanagihara, Takeshi Honda, Shin-Ichiro Nishimura, Toshiomi Yoshida, Tatsuji Seki
Journal of Bioscience and Bioengineering, 92, 6, 569, 574, Society of Fermentation and Bioengineering, 2001, [Peer-reviewed]
English, Scientific journal

Tailored glycopolymers: controlling the carbohydrate-protein interaction based on template effect.
Nagahori N, Nishimura S
Biomacromolecules, 2, 1, 22, 24, 2001, [Peer-reviewed], [Last author, Corresponding author]

A highly practical synthesis of cyclodextrin-based glycoclusters having enhanced affinity with lectins
T Furuike, S Aiba, SI Nishimura
TETRAHEDRON, 56, 51, 9909, 9915, Dec. 2000, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

The interface between innate and acquired immunity: glycolipid antigen presentation by CD1d-expressing dendritic cells to NKT cells induces the differentiation of antigen-specific cytotoxic T lymphocytes
T Nishimura, H Kitamura, K Iwakabe, T Yahata, A Ohta, M Sato, K Takeda, K Okumura, L Van Kaer, T Kawano, M Taniguchi, M Nakui, M Sekimoto, T Koda
INTERNATIONAL IMMUNOLOGY, 12, 7, 987, 994, Jul. 2000, [Peer-reviewed]
English, Scientific journal

The critical role of Th1-dominant immunity in tumor immunology
Takashi Nishimura, Minoru Nakui, Marimo Sato, Kenji Iwakabe, Hidemitsu Kitamura, Masashi Sekimoto, Akio Ohta, Toshiaki Koda, Shinichiro Nishimura
Cancer Chemotherapy and Pharmacology, 46, S1, S52, S61, Springer Science and Business Media LLC, Jul. 2000, [Peer-reviewed]
English, Scientific journal

Design of fluorogenic substrates for continuous assay of sialyltransferase by resonance energy transfer
K Washiya, T Furuike, F Nakajima, YC Lee, SI Nishimura
ANALYTICAL BIOCHEMISTRY, 283, 1, 39, 48, Jul. 2000, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Indispensable role for TNF-α and IFN-γ at the effector phase of liver injury mediated by Th1 cells specific to hepatitis B virus surface antigen
Ohta A, Sekimoto M, Sato M, Koda T, Nishimura S.-I, Iwakura Y, Sekikawa K, Nishimura T
Journal of Immunology, 165, 2, 956, 961, Jul. 2000, [Peer-reviewed]
English

Interleukin-4-dependent induction of preproenkephalin in antigen-specific T helper-type 2 (Th2) cells
T Yahata, C Yahata, A Ohta, M Sekimoto, H Kitamura, K Iwakabe, S Habu, S Azuma, M Nakui, M Sato, T Koda, T Nishimura
JOURNAL OF NEUROIMMUNOLOGY, 105, 2, 103, 108, Jun. 2000, [Peer-reviewed]
English, Scientific journal

Redefined substrate specificity of ST6GalNAc II: A second candidate sialyl-Tn synthase
Mari Kono, Tetsuro Tsuda, Shunichiro Ogata, Shou Takashima, Hong Liu, Toshiro Hamamoto, Steven H. Itzkowitz, Shinichiro Nishimura, Shuichi Tsuji
Biochemical and Biophysical Research Communications, 272, 1, 94, 97, Academic Press Inc., 27 May 2000, [Peer-reviewed]
English, Scientific journal

Web-Structured Films of an Amphiphilic Polymer from Water in Oil Emulsion:  Fabrication and Characterization
Takehiro Nishikawa, Jin Nishida, Ryusuke Ookura, Shin-Ichiro Nishimura, Volker Scheumann, Manfred Zizlsperger, Reinald Lawall, Wolfgang Knoll, Masatsugu Shimomura
Langmuir, 16, 3, 1337, 1342, American Chemical Society (ACS), Feb. 2000, [Peer-reviewed]
English, Scientific journal

α-Galactosylceramide induces early B-cell activation through IL-4 production by NKT cells
Hidemitsu Kitamura, Akio Ohta, Masashi Sekimoto, Marimo Sato, Kenji Iwakabe, Minoru Nakui, Takashi Yahata, Hongxu Meng, Toshiaki Koda, Shin-Ichiro Nishimura, Tetsu Kawano, Masaru Taniguchi, Takashi Nishimura
Cellular Immunology, 199, 1, 37, 42, Academic Press Inc., 10 Jan. 2000, [Peer-reviewed]
English, Scientific journal

Synthetic studies on specific mucins: Antifreeze glygoprotein.
Tachibana, Y, Tsuda, T, Matsubara, N, Tsuda, S, Nishimura, SI
Abstracts of Papers of the American Chemical Society, 219, U244, U244, 2000, [Peer-reviewed]
English, Scientific journal

Bi-fluorescence-labeled maltoheptaoside: Convenient substrate for continual assay of α-amylase
S. I. Nishimura, N. Kimura, K. Matsuoka, Yuan Chuan Lee
Carbohydrate Letters, 4, 2, 77, 84, 2000
English, Scientific journal

Density Dependent Interaction of Polymeric Analogs ofβ-Galactosyl Ceramide with GP120 of Human Immunodeficiency Virus 1　　　　　　　　　　　　　　　
Fujita E, Nishimura S -I
Carbohydr. Lett., 4, 53, 60, 2000, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Chemo-enzymatic synthesis of glycopolymers and sequential glycopeptides bearing lactosamine and sialyl Lewis(x) unit pendant chains
F Sallas, SI Nishimura
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 13, 2091, 2103, 2000, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Synthesis and anti-influenza virus activity of novel glycopolymers having triantennary oligosaccharide branches
T Furuike, S Aiba, T Suzuki, T Takahashi, Y Suzuki, K Yamada, SI Nishimura
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1, 17, 3000, 3005, 2000, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Honeycomb-patterned thin films of amphiphilic polymers as cell culture substrates
Takehiro Nishikawa, Jin Nishida, Ryusuke Ookura, Shin-Ichiro Nishimura, Shigeo Wada, Takeshi Karino, Masatsugu Shimomura
Materials Science and Engineering: C, 8-9, 495, 500, Elsevier BV, Dec. 1999, [Peer-reviewed]
English, Scientific journal

Mesoscopic patterning of cell adhesive substrates as novel biofunctional interfaces
Takehiro Nishikawa, Jin Nishida, Ryusuke Ookura, Shin-Ichiro Nishimura, Shigeo Wada, Takeshi Karino, Masatsugu Shimomura
Materials Science and Engineering: C, 10, 1-2, 141, 146, Elsevier BV, Dec. 1999, [Peer-reviewed]
English, Scientific journal

Cloning and expression of a human gene encoding an N-acetylgalactosamine-alpha2,6-sialyltransferase (ST6GalNAc I): a candidate for synthesis of cancer-associated sialyl-Tn antigens.
Ikehara Y, Kojima N, Kurosawa N, Kudo T, Kono M, Nishihara S, Issiki S, Morozumi K, Itzkowitz S, Tsuda T, Nishimura SI, Tsuji S, Narimatsu H
Glycobiology, 9, 11, 1213, 1224, 11, Nov. 1999, [Peer-reviewed]
Scientific journal

The natural killer T (NKT) cell ligand α-galactosylceramide demonstrates its immunopotentiating effect by inducing interleukin (IL)-12 production by dendritic cells and IL-12 receptor expression on NKT cells
Hidemitsu Kitamura, Kenji Iwakabe, Takashi Yahata, Shin-Ichiro Nishimura, Akio Ohta, Yasushi Ohmi, Marimo Sato, Kazuyoshi Takeda, Ko Okumura, Luc Van Kaer, Tetsu Kawano, Masaru Taniguchi, Takashi Nishimura
Journal of Experimental Medicine, 189, 7, 1121, 1127, 05 Apr. 1999, [Peer-reviewed]
English, Scientific journal

Efficient synthesis of non-natural ganglioside (pseudo-GM3) and fluorescent labelled lysoGM3 on the basis of polymer-assisted enzymatic strategy
K Yamada, S Matsumoto, SI Nishimura
CHEMICAL COMMUNICATIONS, 6, 507, 508, Mar. 1999, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Specific crosslinking of cell adhesive molecules by heterobifunctional glycopeptide synthesised on the basis of chemoenzymatic strategy
SI Nishimura, M Matsuda, H Kitamura, T Nishimura
CHEMICAL COMMUNICATIONS, 15, 1435, 1436, 1999, [Lead author, Corresponding author]
English, Scientific journal

Nonnatural branched polysaccharides: Synthesis and properties of chitin and chitosan having α-mannoside branches
Keisuke Kurita, Kumi Shimada, Yasuhiro Nishiyama, Manabu Shimojoh, Shin-Ichiro Nishimura
Macromolecules, 31, 15, 4764, 4769, American Chemical Society, 28 Jul. 1998, [Peer-reviewed]
English, Scientific journal

Mesoscopic pattern formation of nanostructured polymer assemblies
Norihiko Maruyama, Olaf Karthaus, Kuniharu Ijiro, Masatsugu Shimomura, Takeo Koito, Shinnichiro Nishimura, Tetsuro Sawadaishi, Norio Nishi, Seiichi Tokura
Supramolecular Science, 5, 3-4, 331, 336, Elsevier BV, Jul. 1998, [Peer-reviewed]
English, Scientific journal

Amphiphilic poly(sugar amino acid)s: a novel class of glycoclusters for supramolecular materials
SI Nishimura, S Nomura, K Yamada
CHEMICAL COMMUNICATIONS, 5, 617, 618, Mar. 1998, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Synthesis and self-organisation of new cyclodextrin amphiphile
T Sukegawa, M Matsuda, SI Nishimura, M Shimomura, K Ijiro, O Karthaus
MOLECULAR RECOGNITION AND INCLUSION, 519, 522, 1998
English, International conference proceedings

Regioselective syntheses of sulfated polysaccharides: specific anti-HIV-1 activity of novel chitin sulfates
Shin-Ichiro Nishimura, Hideaki Kai, Katsuhiko Shinada, Takashi Yoshida, Seiichi Tokura, Keisuke Kurita, Hideki Nakashima, Naoki Yamamoto, Toshiyuki Uryu
Carbohydrate Research, 306, 3, 427, 433, Elsevier BV, Jan. 1998, [Peer-reviewed], [Lead author]
English, Scientific journal

Convenient fluorescence probes for the characterisation of glycosyltransferases related to the acrosome reaction　　　　　　　　　　　　　　　
Nishimura S. -I, Washiya K
Zygote, 6, 35, 36, 1998, [Peer-reviewed], [Lead author]
English, Scientific journal

Endotherial cell responses to chitin and its derivatives.
Mori T, Irie Y, Nishimura SI, Tokura S, Matsuura M, Okumura M, Kadosawa T, Fujinaga T
J Biomed Mater Res, 43, 4, 469, 472, 1998

High performance polymer supports for enzyme-assisted synthesis of glycoconjugates
K Yamada, E Fujita, SI Nishimura
CARBOHYDRATE RESEARCH, 305, 3-4, 443, 461, Dec. 1997, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Transfer of ganglioside GM3 oligosaccharide from a water soluble polymer to ceramide by ceramide glycanase. A novel approach for the chemical-enzymatic synthesis of glycosphingolipids
S Nishimura, K Yamada
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 119, 43, 10555, 10556, Oct. 1997, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Swine influenza virus strains recognize sialylsugar chains containing the molecular species of sialic acid predominantly present in the swine tracheal epithelium
T Suzuki, G Horiike, Y Yamazaki, K Kawabe, H Masuda, D Miyamoto, M Matsuda, SI Nishimura, T Yamagata, T Ito, H Kida, Y Kawaoka, Y Suzuki
FEBS LETTERS, 404, 2-3, 192, 196, Mar. 1997, [Peer-reviewed]
English, Scientific journal

Mercapto-chitins: A new type of supports for effective immobilization of acid phosphatase
Keisuke Kurita, Hitoshi Yoshino, Shin-Ichiro Nishimura, Shigeru Ishii, Tomonori Mori, Yasuhiro Nishiyama
Carbohydrate Polymers, 32, 3-4, 171, 175, Elsevier Ltd, 1997, [Peer-reviewed]
English, Scientific journal

Preparation of fluorescence-labeled neoglycolipids for ceramide glycanase assays
Matsuoka K, Nishimura S. I, Lee Y. C
Fluorescence Spectroscopy, 278, 519, 528, 1997, [Peer-reviewed]

Preparation of chitin/polystyrene hybrid materials by efficient graft copolymerization based on mercaptochitin
Keisuke Kurita, Soichiro Hashimoto, Hitoshi Yoshino, Shigeru Ishii, Shin-Ichiro Nishimura
Macromolecules, 29, 6, 1939, 1942, American Chemical Society, 11 Mar. 1996, [Peer-reviewed]
English, Scientific journal

Preparation of novel 1,6-anhydro-beta-lactose derivatives for the synthesis of N-acetyllactosamine-containing oligosaccharides
T Tsuda, T Furuike, SI Nishimura
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 69, 2, 411, 416, Feb. 1996, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Synthetic glycogels for affinity electrophoresis: A facile and efficient method for investigating sugar-lectin interaction
M Ohmae, S Suzuki, S Tokura, N Nishi, SI Nishimura
JOURNAL OF BIOCHEMISTRY, 119, 2, 367, 371, Feb. 1996, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Utilization of DNA as functional materials: Preparation of filters containing DNA insolubilized with alginic acid gel
Norio Nishi, Kazumichi Iwata, Tetsuro Sawadaishi, Shin-Ichiro Nishimura, Seiichi Tokura
International Journal of Biological Macromolecules, 18, 1-2, 149, 150, Elsevier B.V., 1996
English, Scientific journal

Linear polybiurets: Facile synthesis and characteristic properties
Keisuke Kurita, Shin-Ichi Watabe, Shin-Ichiro Nishimura, Shigeru Ishii
Journal of Polymer Science, Part A: Polymer Chemistry, 34, 3, 429, 438, John Wiley and Sons Inc., 1996, [Peer-reviewed]
English, Scientific journal

Synthesis of an antifreeze glycoprotein analogue: Efficient preparation of sequential glycopeptide polymers
Tetsuro Tsuda, Shin-Ichiro Nishimura
Chemical Communications, 24, 2779, 2780, Royal Society of Chemistry, 1996, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Efficient preparation of β-(1←6)-(GlcNAc)2 by enzymatic conversion of chitin and chito-oligosaccharides
Kayo Shimoda, Kohji Nakajima, Yoshiharu Hiratsuka, Shin-Ichiro Nishimura, Keisuke Kurita
Carbohydrate Polymers, 29, 2, 149, 154, Elsevier Ltd, 1996, [Peer-reviewed]
English, Scientific journal

Efficient graft copolymerization of 2-methyl-2-oxazoline onto tosyl- and iodo-chitins in solution
Keisuke Kurita, Soichiro Hashimoto, Shigeru Ishii, Tomonori Mori, Shin-Ichiro Nishimura
Polymer Journal, 28, 8, 686, 689, Society of Polymer Science, 1996, [Peer-reviewed]
English, Scientific journal

An efficient synthesis of sialoglycoconjugates on a peptidase-sensitive polymer support
K Yamada, SI Nishimura
TETRAHEDRON LETTERS, 36, 52, 9493, 9496, Dec. 1995, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Polyisoimides: Synthesis and Evaluation as Efficient Precursors for Polyimides
Keisuke Kurita, Yukiko Suzuki, Takahito Enari, Shigeru Ishii, Shin-Ichiro Nishimura
Macromolecules, 28, 6, 1801, 1806, 01 Nov. 1995, [Peer-reviewed]
English, Scientific journal

SYNTHETIC GLYCOCONJUGATES .6. PREPARATION AND BIOCHEMICAL EVALUATION OF NOVEL CLUSTER-TYPE GLYCOPOLYMERS CONTAINING GAL BETA(1-]4)GLCNAC (N-ACETYLLACTOSAMINE) RESIDUE
T FURUIKE, N NISHI, S TOKURA, SI NISHIMURA
MACROMOLECULES, 28, 21, 7241, 7247, Oct. 1995, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

FLUORESCENCE-LABELED SYNTHETIC GLYCOPOLYMERS - A NEW-TYPE OF SUGAR LIGANDS OF LECTINS
K NAGATA, T FURUIKE, SI NISHIMURA
JOURNAL OF BIOCHEMISTRY, 118, 2, 278, 284, Aug. 1995, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Three-dimensional display of surface cortical perfusion by SPECT: Application in assessing Alzheimer's disease
K. Hashikawa, M. Matsumoto, H. Moriwaki, N. Oku, Y. Okazaki, Y. Seike, T. Uehara, H. Tanabe, Y. Ohie, T. Kamada, T. Nishimura
Journal of Nuclear Medicine, 36, 690, 696, 01 Jan. 1995

A new approach to assay endo-type carbohydrases: Bifluorescent-labeled substrates for glycoamidases and ceramide glycanases
Kyung Bok Lee, Koji Matsuoka, Shin-Ichiro Nishimura, Yuan Chuan Lee
Analytical Biochemistry, 230, 1, 31, 36, 1995, [Peer-reviewed]
English, Scientific journal

GLYCOCONJUGATES .5. POLYMERIC SUGAR LIGANDS AVAILABLE FOR DETERMINING THE BINDING-SPECIFICITY OF LECTINS
MATSUOKA K, NISHIMURA SI
MACROMOLECULES, 28, 8, 2961, 2968, 1995, [Peer-reviewed], [Last author, Corresponding author]

A FACILE AND QUANTITATIVE PREPARATION OF ACTIVATED CYCLIC SUGAR-DERIVATIVES USING HGBR2 AND 2,4,6-COLLIDINE
MATSUOKA K, NISHIMURA SI, LEE YC
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 68, 6, 1715, 1720, 1995, [Peer-reviewed], [Corresponding author]
English

A BI-FLUORESCENCE-LABELED SUBSTRATE FOR CERAMIDE GLYCANASE BASED ON FLUORESCENCE ENERGY-TRANSFER
MATSUOKA K, NISHIMURA SI, LEE YC
CARBOHYDRATE RESEARCH, 276, 1, 31, 42, 1995, [Peer-reviewed], [Corresponding author]

Synthetic Carbohydrate Polymers Containing Trehalose Residues in the Main Chain: Preparation and Characteristic Properties
Keisuke Kurita, Naoko Masuda, Sadafumi Aibe, Kaori Murakami, Shigeru Ishii, Shin-Ichiro Nishimura
Macromolecules, 27, 26, 7544, 7549, 01 Dec. 1994, [Peer-reviewed]
English, Scientific journal

SPLIT-DOSE IODINE-123-IMP SPECT - SEQUENTIAL QUANTITATIVE REGIONAL CEREBRAL BLOOD-FLOW CHANGE WITH PHARMACOLOGICAL INTERVENTION
K HASHIKAWA, M MATSUMOTO, H MORIWAKI, N OKU, Y OKAZAKI, T UEHARA, N HANDA, H KUSUOKA, T KAMADA, T NISHIMURA
JOURNAL OF NUCLEAR MEDICINE, 35, 7, 1226, 1233, Jul. 1994
English, Scientific journal

CARBON-DIOXIDE REACTIVITY BY CONSECUTIVE TECHNETIUM-99M-HMPAO SPECT IN PATIENTS WITH A CHRONICALLY OBSTRUCTED MAJOR CEREBRAL-ARTERY
N OKU, M MATSUMOTO, K HASHIKAWA, H MORIWAKI, Y OKAZAKI, Y SEIKE, N HANDA, T UEHARA, T KAMADA, T NISHIMURA
JOURNAL OF NUCLEAR MEDICINE, 35, 1, 32, 40, Jan. 1994
English, Scientific journal

PREPARATION OF GLYCOPROTEIN MODELS - PENDANT-TYPE OLIGOSACCHARIDE POLYMERS
S NISHIMURA, T FURUIKE, K MATSUOKA
NEOGLYCOCONJUGATES, PT A, 242, 235, 246, 1994, [Peer-reviewed]
English

Biosynthesis of a novel polysaccharide by Acetobacter xylinum
Akihiro Shirai, Mikio Takahashi, Hiroaki Kaneko, Shin-Ichiro Nishimura, Masato Ogawa, Norio Nishi, Seiichi Tokura
International Journal of Biological Macromolecules, 16, 6, 297, 300, 1994, [Peer-reviewed]
English, Scientific journal

Reactivity characteristics of squid β‐chitin as compared with those of shrimp chitin: High potentials of squid chitin as a starting material for facile chemical modifications
Keisuke Kurita, Shigeru Ishii, Koji Tomita, Shin‐Ichiro Nishimura, Kayo Shimoda
Journal of Polymer Science Part A: Polymer Chemistry, 32, 6, 1027, 1032, 1994, [Peer-reviewed]
English, Scientific journal

A novel synthetic approach to a polybiuret from benzyloxyamine and 4,4′‐diphenylmethane diisocyanate
Shigeru Ishii, Shin‐Ichi Watabe, Shin‐Ichiro Nishimura, Keisuke Kurita
Journal of Polymer Science Part A: Polymer Chemistry, 32, 3, 575, 577, 1994, [Peer-reviewed]
English, Scientific journal

Induction of drug specific antibody and the controlled release of drug by 6-O-carboxymethyl-chitin
Tokura Seiichi, Miura Yoshiaki, Johmen Masayoshi, Nishi Norio, Nishimura Shin-Ichiro
Journal of Controlled Release, 28, 1-3, 235, 241, 1994, [Peer-reviewed]
English, Scientific journal

Isoimides and polyisoimides: Facile preparation and quantitative isomerization
Keisuke Kurita, Yukiko Suzuki, Takahito Enari, Masaki Kikuchi, Shin‐Ichiro Nishimura, Shigeru Ishii
Journal of Polymer Science Part A: Polymer Chemistry, 32, 2, 393, 396, 1994, [Peer-reviewed]
English, Scientific journal

SYNTHETIC GLYCOCONJUGATES .4. USE OF OMEGA-(ACRYLAMIDO)ALKYL GLYCOSIDES FOR THE PREPARATION OF CLUSTER GLYCOPOLYMERS
NISHIMURA SI, FURUIKE T, MATSUOKA K
MACROMOLECULES, 27, 18, 4876, 4880, 1994, [Peer-reviewed], [Lead author, Corresponding author]

SYNTHETIC GLYCOCONJUGATES .3. AN EFFICIENT SYNTHESIS OF A GLYCOPROTEIN MODEL HAVING A LE(X)-TYPE TRISACCHARIDE SEQUENCE OF TUMOR-ASSOCIATED CARBOHYDRATE ANTIGEN
NISHIMURA SI, MATSUOKA K, FURUIKE T
MACROMOLECULES, 27, 1, 157, 163, 1994, [Peer-reviewed], [Lead author, Corresponding author]

SYNTHESIS OF BI-FLUORESCENCE-LABELED LACTOSIDE - A SUBSTRATE FOR CONTINUAL ASSAY OF CERAMIDE GLYCANASE
MATSUOKA K, NISHIMURA SI, LEE YC
TETRAHEDRON-ASYMMETRY, 5, 12, 2335, 2338, 1994, [Peer-reviewed]

CHEMOENZYMIC PREPARATION OF A GLYCOCONJUGATE POLYMER HAVING A SIALYLOLIGOSACCHARIDE - NEU5AC-ALPHA(2-]3)GAL-BETA(1-]4)GLCNAC
NISHIMURA SI, LEE KB, MATSUOKA K
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 199, 1, 249, 254, 1994, [Peer-reviewed], [Lead author, Corresponding author]

CHEMOENZYMATIC OLIGOSACCHARIDE SYNTHESIS ON A SOLUBLE POLYMERIC CARRIER
NISHIMURA SI, MATSUOKA K, LEE YC
TETRAHEDRON LETTERS, 35, 31, 5657, 5660, 1994, [Peer-reviewed], [Lead author, Corresponding author]

An Efficient Method for the Syntheses of Novel Amphiphilic Polysaccharides by Regio- and Thermoselective Modifications of Chitosan
NISHIMURA Shin-ichiro, MIURA Yoshiaki, REN Longdi, SATO Manabu, YAMAGISHI Akihiko, NISHI Norio, TOKURA Seiichi, KURITA Keisuke, ISHII Shigeru
Chemistry Letters, 1993, 9, 1623, 1626, The Chemical Society of Japan, 05 Sep. 1993
English, The first example of an unique and efficient method for the preparation of amphiphilic polysaccharides having self-assembling nature is described. Regio- and thermospecific chemical manipulations of a standardized intermediate derived from chitosan markedly facilitated the synthetic procedures of novel types of polysaccharide architectures. Artificial glycolipid-type polymers showed excellent properties to form stable monolayer membranes at air/water interface.

Reactivity characteristics of a new form of chitosan - Facile N-phthaloylation of chitosan prepared from squid β-chitin for effective solubilization
Keisuke Kurita, Koji Tomita, Tomoyoshi Tada, Shin-Ichiro Nishimura, Shigeru Ishii
Polymer Bulletin, 30, 4, 429, 433, Springer-Verlag, Apr. 1993, [Peer-reviewed]
English, Scientific journal

Quantitative assessment of cerebral blood flow by¹²³I-IMP SPECT: Venous sampling method with hand warming in the water bath
H. Moriwaki, M. Matsumoto, K. Hashikawa, N. Oku, Y. Okazaki, N. Handa, K. Kimura, T. Kozuka, T. Kamada, T. Nishimura
Kakuigaku, 30, 481, 488, 01 Jan. 1993

Studies on Functionalized Chitosan Beads. I. Selective Adsorption of Bovine Lactoferrin onto Partially Sulfated Chitosan Beads.
Itoyama Koki, Nishimura Shin-ichiro, Kawamura Yoshihide, Seo Hiroshi, Sato Kaoru, Dosako Shun-ichi, Nishi Norio, Tokura Seiichi
Sen-ito Kogyo, 49, 11, 580, 585, The Society of Fiber Science and Technology, Japan, 1993
English, Porous chitosan beads preparing under the wet condition showed specific capacity to adsorb lactoferrin directly from bovine milk by the partial sulfation reactions. Crosslinking of chitosan beads followed by sulfation was found to give much improved stability and the material exhibited excellent property as an adsorbent material for the affinity chromatography of lactoferrin even in case for higher degrees of sulfation. The amount of lactoferrin bound to the column of chitosan beads was effectively enhanced by increasing CSV value (salt splitting capacity per bed volume of beads) untill 100μeq/mL.

β‐chitin as a convenient starting material for acetolysis for efficient preparation of N‐acetylchitooligosaccharides
Keisuke Kurita, Koji Tomita, Shigeru Ishii, Shin‐Ichiro Nishimura, Kayo Shimoda
Journal of Polymer Science Part A: Polymer Chemistry, 31, 9, 2393, 2395, 1993, [Peer-reviewed]
English, Scientific journal

Squid chitin as a potential alternative chitin source: Deacetylation behavior and characteristic properties
Keisuke Kurita, Koji Tomita, Tomoyoshi Tada, Shigeru Ishii, Shin‐Ichiro Nishimura, Kayo Shimoda
Journal of Polymer Science Part A: Polymer Chemistry, 31, 2, 485, 491, 1993, [Peer-reviewed]
English, Scientific journal

Preparation and biodegradability of chitin derivatives having mercapto groups
Keisuke Kurita, Hitoshi Yoshino, Shin-Ichiro Nishimura, Shigeru Ishii
Carbohydrate Polymers, 20, 4, 239, 245, 1993, [Peer-reviewed]
English, Scientific journal

Preparation of Tosylchitins as Precursors for Facile Chemical Modifications of Chitin
Keisuke Kurita, Hitoshi Yoshino, Koji Yokota, Motonari Ando, Satoshi Inoue, Shigeru Ishii, Shin-Ichiro Nishimura
Macromolecules, 25, 14, 3786, 3790, 01 Jul. 1992, [Peer-reviewed]
English, Scientific journal

Cationic and Radical Graft Copolymerization of Styrene onto Iodochitin
Keisuke Kurita, Satoshi Inoue, Kiichi Yamamura, Hitoshi Yoshino, Shigeru Ishii, Shin-Ichiro Nishimura
Macromolecules, 25, 14, 3791, 3794, 01 Jul. 1992, [Peer-reviewed]
English, Scientific journal

Introduction of poly(L‐alanine) side chains into chitin as versatile spacer arms having a terminal free amino group and immobilization of nadh active sites
Keisuke Kurita, Seiji Iwawaki, Shigeru Ishii, Shin‐Ichiro Nishimura
Journal of Polymer Science Part A: Polymer Chemistry, 30, 4, 685, 688, 1992, [Peer-reviewed]
English, Scientific journal

Polymers Based on p-Aminophenol. 7. New Synthetic Approaches to the Simplest Wholly Aromatic Poly(imide ester) via the Corresponding Poly(amic acid) Precursor
Keisuke Kurita, Naohiro Mikawa, Shigeru Ishii, Shin-Ichiro Nishimura
Macromolecules, 24, 26, 6853, 6855, 01 Dec. 1991, [Peer-reviewed]
English, Scientific journal

Chemospecific Manipulations of a Rigid Polysaccharide: Syntheses of Novel Chitosan Derivatives with Excellent Solubility in Common Organic Solvents by Regioselective Chemical Modifications
Shin-Ichiro Nishimura, Osamu Kohgo, Keisuke Kurita, Hiroyoshi Kuzuhara
Macromolecules, 24, 17, 4745, 4748, 01 Aug. 1991, [Peer-reviewed], [Lead author]
English, Scientific journal

Graft copolymerization of vinyl monomers onto chitin with cerium (IV) ion
Keisuke Kurita, Mitsuo Kawata, Yoshiyuki Koyama, Shin‐Ichiro Nishimura
Journal of Applied Polymer Science, 42, 11, 2885, 2891, 1991, [Peer-reviewed]
English, Scientific journal

Preparation of soluble chitin derivatives as reactive precursors for controlled modifications: Tosyl‐ and iodo‐chitins
Keisuke Kurita, Satoshi Inoue, Shin‐Ichiro Nishimura
Journal of Polymer Science Part A: Polymer Chemistry, 29, 6, 937, 939, 1991, [Peer-reviewed]
English, Scientific journal

Solubilization of a rigid polysaccharide: Controlled partial N-Acetylation of chitosan to develop solubility
Keisuke Kurita, Mami Kamiya, Shin-Ichiro Nishimura
Carbohydrate Polymers, 16, 1, 83, 92, 1991, [Peer-reviewed]
English, Scientific journal

SYNTHETIC GLYCOCONJUGATES .2. NORMAL-PENTENYL GLYCOSIDES AS CONVENIENT MEDIATORS FOR THE SYNTHESES OF NEW TYPES OF GLYCOPROTEIN MODELS
NISHIMURA SI, MATSUOKA K, FURUIKE T
MACROMOLECULES, 24, 15, 4236, 4241, 1991, [Peer-reviewed], [Lead author, Corresponding author]

Synthesis of a peripheral trisaccharide sequence of lutropin, a pituitary glycoprotein hormone; use of chitobiose as a key starting material
Shin-Ichiro Nishimura, Hiroyoshi Kuzuhara
Carbohydrate Research, 206, 2, 207, 217, 10 Oct. 1990, [Peer-reviewed], [Lead author]
English, Scientific journal

Preparations of soluble chitin derivatives and the modifications to branched chitins　　　　　　　　　　　　　　　
Keisuke Kurita, Shin Ichiro Nishimura, Yoshiyuki Koyama, Satoshi Inoue, Osamu Kohgo
American Chemical Society, Polymer Preprints, Division of Polymer Chemistry, 31, 1, 624, 625, Publ by ACS, Apr. 1990
English, International conference proceedings

Synthetic Glycoconjugates: Simple and Potential Glycoprotein Models Containing Pendant N-Acetyl-D-glucosamine and N.N’-Diacetylchitobiose
Shin-Ichiro Nishimura, Koji Matsuoka, Keisuke Kurita
Macromolecules, 23, 18, 4182, 4184, 1990, [Peer-reviewed], [Lead author]
English, Scientific journal

((Diethylamino) ethyl) chitins: Preparation and Properties of Novel Aminated Chitin Derivatives
Keisuke Kurita, Yoshiyuki Koyama, Satoshi Inoue, Shin-Ichiro Nishimura
Macromolecules, 23, 11, 2865, 2869, 1990, [Peer-reviewed]
English, Scientific journal

Polymers Based on p-Aminophenol. 6. Facile Synthesis of the Simplest Wholly Aromatic Poly(imide-ester) by Pyrolytic Polymerization of Monomers Containing Preformed Ester Linkages
Keisuke Kurita, Naohiro Mikawa, Yoshiyuki Koyama, Shin-Ichiro Nishimura
Macromolecules, 23, 10, 2605, 2609, 1990, [Peer-reviewed]
English, Scientific journal

N-hydroxyacylation of chitosan with lactones
Kurita Keisuke, Takanobu Takeda, Shinichiro Nishimura
Polymer Journal, 22, 5, 429, 434, 1990, [Peer-reviewed]
English, Scientific journal

Peracetylated chitobiose: Preparation by specific degradations of chitin, and chemical manipulations
Shin-Ichiro Nishimura, Hiroyoshi Kuzuhara, Yasuyuki Takiguchi, Kenzo Shimahara
Carbohydrate Research, 194, C, 223, 231, 01 Dec. 1989, [Peer-reviewed], [Lead author]
English, Scientific journal

TRANSFORMATION OF NIH3T3 CELLS WITH SYNTHETIC C-HA-RAS GENES
H KAMIYA, K MIURA, N OHTOMO, T KODA, M KAKINUMA, S NISHIMURA, E OHTSUKA
JAPANESE JOURNAL OF CANCER RESEARCH, 80, 3, 200, 203, Mar. 1989, [Peer-reviewed]
English

PREPARATION AND ANTITHROMBOGENIC ACTIVITIES OF HEPARINOID FROM 6-O-(CARBOXYMETHYL)CHITIN
S NISHIMURA, S TOKURA
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 9, 4, 225, 232, Aug. 1987, [Peer-reviewed], [Lead author]
English, Scientific journal

Induction of methamphetamine-specific antibody using biodegradable carboxymethyl-chitin
Seiichi Tokura, Osatoshi Hasegawa, Shin-Ichiro Nishimura, Norio Nishi, Takehiko Takatori
Analytical Biochemistry, 161, 1, 117, 122, 15 Feb. 1987
English, Scientific journal

Effect of multiporous microspheres derived from chitin and partially deacetylated chitin on the activation of mouse peritoneal macrophages
Keiko Nishimura, Shin-ichiro Nishimura, Hiroshi Seo, Norio Nishi, Seiichi Tokura, Ichiro Azuma
Vaccine, 5, 2, 136, 140, 1987
English, Scientific journal

Interaction of chitin heparinoids with bovine antithrombin III
Shin-ichiro Nishimura, Norio Nishi, Seiichi Tokura
International Journal of Biological Macromolecules, 9, 5, 305, 307, 1987
English, Scientific journal

Inhibition of the hydrolytic activity of thrombin by chitin heparinoids
Shin-Ichiro Nishimura, Norio Nishi, Seiichi Tokura, Wesley Okiei, Oyin Somorin
Carbohydrate Research, 156, C, 286, 292, 15 Nov. 1986
English, Scientific journal

Bioactive chitin derivatives. Activation of mouse-peritoneal macrophages by O-(carboxymethyl)chitins
Shin-Ichiro Nishimura, Norio Nishi, Seiichi Tokura, Keiko Nishimura, Ichiro Azuma
Carbohydrate Research, 146, 2, 251, 258, 01 Feb. 1986
English, Scientific journal

Highly phosphorylated derivatives of chitin, partially deacetylated chitin and chitosan as new functional polymers: preparation and characterization
Norio Nishi, Akira Ebina, Shin-ichiro Nishimura, Akihiro Tsutsumi, Osatoshi Hasegawa, Seiichi Tokura
International Journal of Biological Macromolecules, 8, 5, 311, 317, 1986
English, Scientific journal

Macrophage activation with multi‐porous beads prepared from partially deacetylated chitin
Keiko Nishimura, Shin‐Ichiro Nishimura, Hiroshi Seo, Norio Nishi, Seiichi Tokura, Ichiro Azuma
Journal of Biomedical Materials Research, 20, 9, 1359, 1372, 1986
English, Scientific journal

Adjuvant activity of chitin derivatives in mice and guinea-pigs
Keiko Nishimura, Shin-ichiro Nishimura, Norio Nishi, Fumio Numata, Yukiko Tone, Seiichi Tokura, Ichiro Azuma
Vaccine, 3, 5, 379, 384, 1985
English, Scientific journal

Adhesion behaviour of murine lymphocytes on the surface of fibrous chitin and its derivatives
Shin-ichiro Nishimura, Norio Nishi, Seiichi Tokura
International Journal of Biological Macromolecules, 7, 2, 100, 104, 1985
English, Scientific journal

Preparation and characterization of water-soluble chitin phosphate
Norio Nishi, Shin-ichiro Nishimura, Akira Ebina, Akihiro Tsutsumi, Seiichi Tokura
International Journal of Biological Macromolecules, 6, 1, 53, 54, 1984
English, Scientific journal

THE ADSORPTION OF BOVINE BLOOD PROTEINS ONTO THE SURFACE OF O-(CARBOXYMETHYL)CHITIN
S NISHIMURA, Y IKEUCHI, S TOKURA
CARBOHYDRATE RESEARCH, 134, 2, 305, 312, 1984, [Peer-reviewed], [Lead author]
English, Scientific journal

Studies on Chitin. VII. Preparations of p-Substituted Benzoylchitins
Norio Nishi, Hiroyuki Ohnuma, Shin-Ichiro Nishimura, Oyin Somorin, Seiichi Tokura
Polymer Journal, 14, 11, 913, 919, 1982, [Peer-reviewed]
English, Scientific journal

ナノ粒子のがん指向性を向上させる膜固定化ミニ抗体の開発
佐藤史菜, 涌井初, 横井康広, 横井康広, 平根望巳, 尾瀬農之, 尾瀬農之, 西村紳一郎, 西村紳一郎, 西村紳一郎, 日本蛋白質科学会年会(Web), 25th, 2025

スパイクタンパク質の「動的エピトープ」は新型コロナウイルスの弱点？　　　　　　　　　　　　　　　
西村紳一郎, 涌井初, 横井康広, 臨床化学, 50, 4, 2021, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

理学部で自由に研究できるという最高の幸運に感謝　　　　　　　　　　　　　　　
西村紳一郎, 彩：理学部創立90周年記念誌, 2020, [Invited], [Lead author]
Japanese, Introduction research institution

現場のアンメットニーズを考える　　　　　　　　　　　　　　　
西村紳一郎, 臨床化学, 47, 342, 343, Jul. 2018, [Invited], [Lead author]
Japanese, Introduction scientific journal

Screening for serum N-glycan marker associated with early antibody-mediated rejection in patients receiving a living donor kidney transplant
野呂 大輔, 米山 徹, 畠山 真吾, 飛澤 悠葵, 盛 和行, 橋本 安弘, 古家 琢也, 田中 誠一, 西村 紳一郎, 佐々木 秀郎, 齋藤 満, 原田 浩, 力石 辰也, 石田 英樹, 田邉 一成, 佐藤 滋, 大山 力, 日本臨床腎移植学会雑誌 = Journal of Japanese Society for Clinical Renal Transplantation, 5, 1, 71, 82, Jul. 2017
日本臨床腎移植学会, Japanese

NOTCH1EGFドメインにおけるO-結合型糖鎖修飾の構造および機能
横井康広, 西村紳一郎, 日本化学会春季年会講演予稿集(CD-ROM), 97th, 2017

グライコブロッティング法に基づくリポソーム及びリポたんぱく質中酸化脂質の迅速解析
古川貴之, 比能洋, 武田晴治, 千葉仁志, 西村紳一郎, HUI Shu-Ping, JSBMS Letters, 42, Supplement, 2017

グライコブロッティング法によるリポタンパク質中の脂質アルデヒド分析
古川貴之, 比能洋, 武田晴治, 千葉仁志, 西村紳一郎, 惠淑萍, 臨床化学, 46, 2017

網羅的糖鎖解析による肝細胞癌の浸潤能と糖鎖異常の検討
高橋秀徳, 神山俊哉, 柿坂達彦, 相山健, 島田慎吾, 若山顕治, 永生高弘, 折茂達也, 蒲池浩文, 横尾英樹, 西村紳一郎, 武冨紹信, 日本外科学会定期学術集会(Web), 117th, ROMBUNNO.PS‐076‐4 (WEB ONLY), 2017
Japanese

脂質アルデヒド類の新規分析法 グライコブロッティング法によるアプローチ 合成標品からリポソームまで　　　　　　　　　　　　　　　
古川 貴之, 惠 淑萍, 比能 洋, 西村 紳一郎, 千葉 仁志, 臨床化学, 45, Suppl.1, 167, 167, Oct. 2016
(一社)日本臨床化学会, Japanese

糖尿病網膜症の硝子体および血漿におけるN型糖鎖プロファイル　　　　　　　　　　　　　　　
稲福 沙織, 野田 航介, 天野 麻穂, 大橋 哲, 齋藤 航, 村田 美幸, 神田 敦宏, 西村 紳一郎, 石田 晋, 日本糖尿病眼学会誌, 20, 114, 114, Mar. 2016
日本糖尿病眼学会, Japanese

網羅的糖鎖解析による肝細胞癌の浸潤能と糖鎖異常の検討
高橋秀徳, 神山俊哉, 柿坂達彦, 相山健, 島田慎吾, 永生高広, 若山顕治, 折茂達也, 蒲池浩文, 横尾英樹, 西村紳一郎, 武冨紹信, 日本消化器癌発生学会総会プログラム・抄録集, 27th, 104, 2016
Japanese

肝癌細胞株におけるuPAの発現変化に伴う浸潤能変化と糖鎖異常解析
高橋秀徳, 柿坂達彦, 神山俊哉, 相山健, 島田慎吾, 若山顕治, 折茂達也, 敦賀陽介, 蒲池浩文, 横尾英樹, 西村紳一郎, 武冨紹信, 日本外科学会定期学術集会(Web), 116th, OP‐090‐1 (WEB ONLY), 2016
Japanese

グライコブロッティング法を用いた脂質アルデヒドの定量測定法の開発
古川貴之, 惠淑萍, 比能洋, 西村紳一郎, 千葉仁志, 臨床化学, 44, 209, 07 Oct. 2015
Japanese

Glycoblotting法を用いた牛乳中遊離オリゴ糖の網羅的定量解析　　　　　　　　　　　　　　　
山本 祐輔, 古川 貴之, 惠 淑萍, 比能 洋, 西村 紳一郎, 千葉 仁志, 日本未病システム学会学術総会抄録集, 22回, 134, 134, Sep. 2015
(一社)日本未病システム学会, Japanese

ブロッティング技術に基づく脂質アルデヒド類の網羅的定量解析　　　　　　　　　　　　　　　
古川 貴之, 惠 淑萍, 比能 洋, 西村 紳一郎, 千葉 仁志, 日本未病システム学会学術総会抄録集, 22回, 136, 136, Sep. 2015
(一社)日本未病システム学会, Japanese

私の自慢 継続は力なり 点が線になるとき
西村 紳一郎, 化学と工業 = Chemistry & chemical industry, 68, 8, 726, 728, Aug. 2015
日本化学会, Japanese

O-mannosylated glycan induced conformational alteration of alpha-dystroglycan fragment
Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015
English, Summary international conference

Structural insight into glycosylated human Notch1 EGF12 analogs
Shun Hayakawa, Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015
English, Summary international conference

Intracellular traffic of cell surface mimetic quantum dots-anchored glycopeptides
Shin-Ichiro Nishimura, Roger Tan, Kentaro Naruchi, Maho Amano, Hiroshi Hinou, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015
English, Summary international conference

糖尿病網膜症の硝子体および血漿におけるN型糖鎖の網羅的解析　　　　　　　　　　　　　　　
稲福 沙織, 野田 航介, 天野 麻穂, 大橋 哲, 吉澤 史子, 齋藤 航, 村田 美幸, 神田 敦宏, 西村 紳一郎, 石田 晋, 日本眼科学会雑誌, 119, 臨増, 160, 160, Mar. 2015
(公財)日本眼科学会, Japanese

糖負荷刺激によるシアル酸転移酵素の誘導　　　　　　　　　　　　　　　
稲福 沙織, 野田 航介, 天野 麻穂, 大橋 哲, 齋藤 航, 村田 美幸, 神田 敦宏, 西村 紳一郎, 石田 晋, 日本眼科学会雑誌, 119, 臨増, 199, 199, Mar. 2015
(公財)日本眼科学会, Japanese

糖尿病モデルマウスの比較糖鎖解析
坂西裕介, 比能洋, 天野麻穂, 幸田敏明, 上村佳子, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 49th, 2015

アルコールを用いたペプチド環化反応のpKa依存性について
酒井隆史, 比能洋, 西村紳一郎, 高分子学会予稿集(CD-ROM), 64, 1, 2015

タウタンパクフラグメントの合成および立体構造解析
横井康広, 早川瞬, 比能洋, 西村紳一郎, 高分子学会予稿集(CD-ROM), 64, 1, 2015

CMPアジド化シアル酸の合成と糖タンパク質末端の改変
飯田優香, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 49th, 2015

DETECTION OF HEMAGGLUTININ DISSOCIATED FROM INFLUENZA VIRUS USING GLYCAN-IMMOBILIZED FIELD EFFECT TRANSISTOR
南部谷俊介, 前川達洋, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 中西卓也, 逢坂哲彌, 逢坂哲彌, Chemical Sensors, 31, Supplement A, 2015

糖鎖固定化電界効果トランジスタによるインフルエンザウイルスから分離したヘマグルチニンの検出
南部谷俊介, 前川達洋, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 中西卓也, 逢坂哲彌, 電気化学会大会講演要旨集(CD-ROM), 82nd, 2015

肝癌細胞株における浸潤能と糖鎖異常の解析
高橋秀徳, 柿坂達彦, 神山俊哉, 相山健, 島田慎吾, 若山顕治, 折茂達也, 敦賀陽介, 蒲池浩文, 横尾英樹, 田中誠一, 西村紳一郎, 武冨紹信, 日本癌治療学会学術集会(Web), 53rd, P131‐1 (WEB ONLY), 2015
Japanese

肝疾患の病態と糖鎖抗原の意義 網羅的糖鎖解析による肝細胞癌バイオマーカーの検討　　　　　　　　　　　　　　　
宮原 孝治, 能祖 一裕, 森元 裕貴, 衣笠 秀明, 和田 望, 竹内 康人, 安中 哲也, 桑木 健志, 大西 秀樹, 池田 房雄, 三宅 康弘, 中村 進一郎, 白羽 英則, 高木 章乃夫, 天野 麻穂, 西村 紳一郎, 山本 和秀, 肝臓, 55, Suppl.1, A154, A154, Apr. 2014
(一社)日本肝臓学会, Japanese

量子ドットに提示したCMPシアル酸のライブセルイメージング
平根望巳, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 48th, 2014

O-Mannose型糖鎖を有する糖ペプチドライブラリの構築及び機能解析に関する研究
菊地星矢, 松下隆彦, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 48th, 2014

ヒトIgG1抗体に存在するBisect型N-Glycanの効率合成と糖ペプチド合成への応用
石川明香里, RAVI KUMAR H.V., 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 48th, 2014

糖タンパク質の糖鎖を付け替える 均一な糖鎖構造をもつ糖タンパク質の効率的合成
比能洋, 西村紳一郎, 化学, 69, 1, 2014

Prediction of Survival in Patients with Hepatocellular Carcinoma Treated with Sorafenib by Comprehensive Serum Glycan Analysis
Kazuhiro Nouso, Koji Miyahara, Yuuki Morimoto, Yasuto Takeuchi, Hiroaki Hagihara, Kenji Kuwaki, Hideki Onishi, Fusao Ikeda, Yasuhiro Miyake, Shinichiro Nakamura, Hidenori Shiraha, Akinobu Takaki, Koichi Takaguchi, Takahisa Sato, Shinpei Sato, Shuntaro Obi, Kazuko Hirose, Maho Amano, Shin-Ichiro Nishimura, Kazuhide Yamamoto, HEPATOLOGY, 58, 1242A, 1242A, Oct. 2013
English, Summary international conference

Highly efficient synthesis of hyperbranched N-glycans from locust bean gum
H. V. Ravi Kuma, Kentaro Naruchi, Risho Miyoshi, Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 246, Sep. 2013
English, Summary international conference

血清糖鎖マーカーによる進行肝細胞癌の予後予測　　　　　　　　　　　　　　　
宮原 孝治, 能祖 一裕, 森元 裕貴, 和田 望, 竹内 康人, 萩原 宏明, 桑木 健志, 大西 秀樹, 中村 進一郎, 白羽 英則, 天野 麻穂, 西村 紳一郎, 山本 和秀, 肝臓, 54, Suppl.2, A638, A638, Sep. 2013
(一社)日本肝臓学会, Japanese

High-Throughput Antigen Profiling for MUC1 Glycoprotein Antibody
F. Garcia-Martin, T. Matsushita, H. Hinou, S-I Nishimura, BIOPOLYMERS, 100, 3, 269, 269, May 2013
English, Summary international conference

ABERRANT GLYCOSYLATION OF N-GLYCAN ON IMMUNOGLOBULIN CLOSELY RELATES SURVIVAL OF THE PATIENTS WITH IN RENAL CELL CARCINOMA
Shingo Hatakeyama, Yuki Tobisawa, Tohru Yoneyama, Yuichiro Suzuki, Hayato Yamamoto, Kazuyuki Mori, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Norihiko Tsuchiya, Tomonori Habuchi, Maho Amano, Shin-Ichiro Nishimura, Chikara Ohyama, JOURNAL OF UROLOGY, 189, 4, E186, E187, Apr. 2013
English, Summary international conference

ぶどう膜炎における網羅的糖鎖解析　　　　　　　　　　　　　　　
南場 研一, 北市 伸義, 安藤 亮, 竹本 裕子, 水内 一臣, 堀江 幸弘, 大野 重昭, 天野 麻穂, 西村 紳一郎, 石田 晋, 日本眼科学会雑誌, 117, 臨増, 318, 318, Mar. 2013
(公財)日本眼科学会, Japanese

ケイ素保護基を用いた糖ペプチドの迅速固相合成法
藤井宏圭, 松下隆彦, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013

糖残基を有するヒトNotch-1EGF-12ドメインの合成と配座解析
脇本行彦, 松下隆彦, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013

O-Mannose型糖鎖を有するαジストログリカン糖ペプチドライブラリの合成と相互作用解析
菊地星矢, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 93rd, 3, 2013

O-マンノース含有ジストログリカン関連糖ペプチドの合成と相互作用解析
比能洋, 比能洋, 菊地星矢, 松下隆彦, 西村紳一郎, 西村紳一郎, 高分子学会予稿集(CD-ROM), 62, 1, 2013

ムチン型糖タンパク質におけるTTXモチーフのNMR構造解析
早川瞬, 松下隆彦, GARCIA-MARTIN Fayna, 成地健太郎, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013

O-Mannose型糖鎖を有するαジストログリカン糖ペプチドライブラリの合成と相互作用解析
菊地星矢, 比能洋, 比能洋, 松下隆彦, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 47th, 2013

Autoantibodies to cancer-associated MUC1 fragments in healthy human sera discovered by high performance glycopeptide microarray platform
Nishimura S. I, Matsushita T, Takada W, Igarashi K, Naruchi K, Garcia-Martin F, Amano M, Hinou H, Abstracts of Papers of the American Chemical Society, 246, 2013, [Peer-reviewed]

Serum N-glycan profiling has potential to predict cardiovascular disease (CVD) event and prognosis in hemodialysis (HD) patients
Shingo Hatakeyama, Yuki Tobisawa, Tohru Yoneyama, Maho Amano, Shin-Ichiro Nishimura, Chikara Ohyama, GLYCOBIOLOGY, 22, 11, 1558, 1559, Nov. 2012
English, Summary international conference

糖鎖化学 それぞれの大志を抱け! : 留学先での新渡戸稲造博士との出会い (特集 日本の化学 : その元気度は?) -- (各分野の元気度は?)
西村 紳一郎, 化学, 67, 4, 25, 27, Apr. 2012
化学同人, Japanese

グライコブロッティング法を用いた異なる種類のヒアルロン酸分解酵素生成ヒアルロン酸断片の同定
ARAI MISAKI, FURUKAWA TAKAYUKI, GARUSHIA FAINA, HINO HIROSHI, AMANO MAHO, NISHIMURA SHIN'ICHIRO, 日本薬学会年会要旨集, 132nd, 3, 185, 05 Mar. 2012
Japanese

¹C₄配座を有する新奇グルクロン酸糖供与体に関する研究
FURUKAWA TAKAYUKI, HINO HIROSHI, NISHIMURA SHIN'ICHIRO, 日本薬学会年会要旨集, 132nd, 2, 238, 05 Mar. 2012
Japanese

Rapid Discovery of MUC1 Epitopes by Glycopeptide Combinatorial Chemistry
GARCIA-MARTIN Fayna, MATSUSHITA Takahiko, HINOU Hiroshi, NISHIMURA Shin-Ichiro, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 67, 68, 01 Mar. 2012
English

糖鎖捕捉反応を用いた新規糖脂質ライブラリの構築と溶液中における構造解析
石田純也, 比能洋, 西村紳一郎, 日本薬学会年会要旨集, 132nd, 2, 2012

ムチン型糖ペプチドにおけるTTXモチーフのNMR構造解析
早川瞬, 松下隆彦, 比能洋, 西村紳一郎, 日本薬学会年会要旨集, 132nd, 2, 2012

Exploration of Disease-Specific Epitopes by using MUC1 Glycopeptide Microarray
Wataru Takada, Takahiko Matsushita, Hiroshi Hinou, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 21, 11, 1509, 1510, Nov. 2011
English, Summary international conference

Development and Application of Versatile Glycan Microarray
Kohta Igarashi, Wataru Takada, Hiroki Abe, Takahiko Matsushita, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 21, 11, 1510, 1510, Nov. 2011
English, Summary international conference

Glycoblotting 法による健常成人血清中の糖鎖プロファイル解析
木村 聡, 天野 麻穂, 西村 紳一郎, 臨床病理, 59, 6, 25 Jun. 2011
Japanese

HIGH THROUGH PUT, COMPREHENSIVE AND QUANTITATIVE GLYCOMICS OF WHOLE SERUM GLYCOPROTEINS FOR DIAGNOSIS OF RENAL CELL CARCINOMA
Shingo Hatakeyama, Maho Amano, Megumi Tsushima, Hatato Yamamoto, Tohru Yoneyama, Yusuke Ishibashi, Hiromi Murasawa, Kengo Imanishi, Teppei Okamoto, Noriko Tokui, Yuichiro Suzuki, Naoki Sugiyama, Sigemasa Kudo, Takahiro Yoneyama, Takuya Koie, Norihiko Tsuchiya, Tomonori Habuchi, Shin-Ichiro Nishimura, Chikara Ohyama, JOURNAL OF UROLOGY, 185, 4, E385, E385, Apr. 2011
English, Summary international conference

配座変換を鍵戦略とした新規グルクロン酸及びイズロン酸糖供与体の開発
FURUKAWA TAKAYUKI, HINO HIROSHI, NISHIMURA SHIN'ICHIRO, 日本化学会講演予稿集, 91st, 4, 1146, 11 Mar. 2011
Japanese

Novel Coupling Method for the Incorporation of 'Complex' Amino acid
GARCIA-MARTIN Fayna, HINOU Hiroshi, MATSUSHITA Takahiko, NISHIMURA Shin-Ichiro, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2010, 204, 204, 01 Mar. 2011
English

Significance of posttranslational modifications uncovered by glycoblotting method and development of large-scale glycomics
AMANO Maho, MIURA Yoshiaki, NISHIMURA Shin-Ichiro, 生化學, 83, 1, 5, 12, 25 Jan. 2011
日本生化学会, Japanese

不凍糖タンパク質の構造と機能の解明
八須匡和, 西村紳一郎, 中部化学関係学協会支部連合秋季大会講演予稿集, 42nd, 2011

APP-013 腎細胞癌における血清N-結合型糖鎖の網羅的解析による新規バイオマーカーの検索(総会賞応募ポスター,第99回日本泌尿器科学会総会)
畠山 真吾, 舟生 富壽, 天野 麻穂, 西村 紳一郎, 大山 力, 山本 隼人, 米山 徹, 盛 和行, 工藤 茂将, 米山 高弘, 古家 琢也, 神村 典孝, 對馬 惠, 日泌尿会誌, 102, 2, 2011
一般社団法人 日本泌尿器科学会, Japanese

Plastid targeting of NPP glycoprotein from the golgi apparatus through the secretory pathway in rice cell
Kaneko Kentaro, Kosyu Tsutomu, Umezawa Yukiho, Ishimoto Takuya, Amano Maho, Nishimura Shin-Ichro, Mitsui Toshiaki, Abstracts for Annual Meeting of Japanese Proteomics Society, 2011, 0, 139, 139, 2011
日本プロテオーム学会（日本ヒトプロテオーム機構）, Japanese

N-glycome of rice plastidial nucleotide pyrophosphatase/phosphodiesterases: Functional glycoproteins are transported from the late compartments of the Golgi apparatus to the plastid
Mitsui Toshiaki, Pozueta-Romero Javier, Kaneko Kentaro, Koushu Tsutomu, Umezawa Sachiho, Koga-Kitajima Aya, Shiraya Takeshi, Hirose Kazuko, Amano Maho, Nishimura Shin-ichiro, Abstracts for Annual Meeting of Japanese Proteomics Society, 2011, 0, 65, 65, 2011
日本プロテオーム学会（日本ヒトプロテオーム機構）, Japanese

新規糖脂質ライブラリの構築と構造解析
石田純也, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 45th, 2011

シアリダーゼ阻害剤の探索技術に関する研究
三好利尚, 甲斐宏一, 高須康明, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 45th, 2011

新規糖脂質類縁体ライブラリの構築と構造解析
石田純也, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 91st, 3, 2011

環状構造を有する新奇シアリダーゼ自殺基質型阻害剤の開発
甲斐宏一, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 91st, 3, 2011

疾患特異的エピトープ探索を指向したMUC1糖ペプチドマイクロアレイの開発
松下隆彦, 高田渉, 比能洋, 西村紳一郎, 日本糖質学会年会要旨集, 30th, 2011

バイオサイエンス グライコブロッティング法と糖鎖自動抽出装置(SweetBlot)による一滴の血清からの糖鎖バイオマーカー探索
古川 潤一, 西村 紳一郎, 未来材料, 10, 11, 48, 54, Nov. 2010
エヌ・ティー・エス, Japanese

Systematic Determination of Multiple O-Glycosylation Sites in Proteins using Electron Capture Dissociation (ECD) in a Linear Ion Trap
Takeshi Sakamoto, Naomi Manri, Akihito Kaneko, Atsumu Hirabayashi, Takashi Baba, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 20, 11, 1467, 1468, Nov. 2010
English, Summary international conference

Serum N-Glycan Profile in Renal Cell Carcinoma
Shingo Hatakeyama, Maho Amano, Naoki Fujita, Noriko Tokui, Toru Yoneyama, Kengo Imanishi, Hayato Yamamoto, Shigemasa Kudo, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Noritaka Kamimura, Shin-Ichiro Nishimura, Chikara Ohyama, GLYCOBIOLOGY, 20, 11, 1501, 1501, Nov. 2010
English, Summary international conference

高反応性かつ高立体選択性を有する新規グルクロン酸供与体の開発
FURUKAWA TAKAYUKI, HINO HIROSHI, NISHIMURA SHIN'ICHIRO, 日本化学会講演予稿集, 90th, 4, 1027, 12 Mar. 2010
Japanese

Chemo-enzymatic synthesis of mucin-type glycopeptides using sugar-protective groups and ppGalNAcT-2
Yayoi Yoshimura, Takahiko Matsushita, Naoki Fujitani, Fujihira Haruhiko, Yasuhiro Takegawa, Naomi Manri, Akihito Kaneko, Takeshi Sakamoto, Xiao-Dong Gao, Hiroshi Hinou, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 239, Mar. 2010
English, Summary international conference

化学-酵素法による複合糖質の自動合成
八須匡和, 松下隆彦, 清水弘樹, 長島生, 作田智美, 畑中研一, 西村紳一郎, 西村紳一郎, 日本化学会講演予稿集, 90th, 3, 2010

Discovery of cancer-specific glycopeptide epitope and development of disease-related biomarkers -----Learning from the epitope mapping of anti-KL-6 monoclonal antibody-----
Nishimura Shin-Ichiro, Japan Journal of Molecular Tumor Marker Research, 25, 3, 4, 2010
日本分子腫瘍マーカー研究会, Japanese

新奇シアリダーゼ自殺基質型阻害剤の開発
甲斐宏一, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 90th, 3, 2010

Synthesis, Folding and Structural Insights Into Glycosylated EGF12 on Mouse Notch-1 Receptor
Kensaku Hosoguchi, Kazumi Shimizu, Naoki Fujitani, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 19, 11, 1350, 1350, Nov. 2009
English, Summary international conference

Large-Scale Serum Glycomics of CFG KO Mice Based on the Glycoblotting Method
Maho Amano, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 19, 11, 1303, 1303, Nov. 2009
English, Summary international conference

Glycoform-Focused Reverse Genomics (GFRG) in Mouse Ovulation
Maho Amano, Mari Yokoyama, Masaki Kurogochi, Yasuhiro Takegawa, Noriko Nagahori, Tadashi Yamashita, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 19, 11, 1340, 1340, Nov. 2009
English, Summary international conference

NMRによるリニア不凍糖タンパク質(AFGP)のコンフォメーション解析
泉龍孝, 藤谷直樹, 松下隆彦, 比能洋, 高道学, 西宮佳志, 津田栄, 西村紳一郎, 日本糖質学会年会要旨集, 29th, 85, 10 Aug. 2009
Japanese

【ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術 細胞の生存、増殖、機能のコントロールから創薬研究、再生医療まで】細胞周辺環境のための材料科学技術 高分子 糖鎖による軟骨細胞機能制御の可能性 軟骨変性に伴うN-結合型糖鎖の変化より　　　　　　　　　　　　　　　
岩崎 倫政, 松橋 智弥, 瓜田 淳, 高畑 雅彦, 三浪 明男, 西村 紳一郎, 遺伝子医学MOOK, 別冊, ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術, 55, 59, Aug. 2009
(株)メディカルドゥ, Japanese

Development and application of glycan chip & microarray: approach for exploring the potential biomarkers
五十嵐 幸太, 松下 隆彦, 西村 紳一郎, Bio industry, 26, 7, 63, 70, Jul. 2009
シーエムシー出版, Japanese

Chemoenzymatic Synthesis of Glycosylated Glucagon-like Peptide 1: Effect of Glycosylation on Proteolytic Resistance and in Vivo Blood Glucose-Lowering Activity
Taichi Ueda, Kazuyoshi Tomita, Yoshihide Notsu, Takaomi Ito, Masataka Fumoto, Tomoaki Takakura, Hirofumi Nagatome, Akio Takimoto, Shin-Ichi Mihara, Hiroko Togame, Keiko Kawamoto, Takanori Iwasaki, Kenji Asakura, Takeo Oshima, Kohji Hanasaki, Shin-Ichiro Nishimura, Hirosato Kondo, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131, 17, 6237, 6245, May 2009
English

mechanism-based labeling of proteins using suicide substrate
FURUKAWA TAKAYUKI, HINO HIROSHI, FENG FEI, NISHIMURA SHIN'ICHIRO, 日本化学会講演予稿集, 89th, 2, 1529, 13 Mar. 2009
Japanese

Structual study of glycosylated EGF12 on mouse Notch-1 receptor
細口健作, 蛭間(清水)和美, 藤谷直樹, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 1314, 13 Mar. 2009
Japanese

ペプチド型プローブの迅速探索法
FURUKAWA TAKAYUKI, HINO HIROSHI, NISHIMURA SHIN'ICHIRO, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 51, 03 Feb. 2009
Japanese

ppGalNAcT阻害剤に関する研究
阿部皓基, 比能洋, 比能洋, 古川潤一, 前田哲宏, 細口健作, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009

ヒトシアリダーゼの発現と機能解析
奥山力, 高暁冬, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009

Development of environment-conscious process for glycosylation-Improvement of solid super acid promoter, ZrO₂/SO₄^2--
齋藤直裕, 前田哲宏, 松田匡雄, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 1, 2009

Research on the development of novel structures-based Influenza virus sialidase inhibitors
佐藤公法, 比能洋, 高須康明, 黒河内政樹, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009

Study of an Inhibitor of ppGalNAcTs
阿部皓基, 比能洋, 古川潤一, 前田哲宏, 細口健作, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009

Regulation of Glycan Biosynthetic Mechanism by Glycomimics
日向寺慧, 羽藤愛美, 天野麻穂, FENG Fei, 三浦嘉晃, 篠原康郎, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009

Solid phase glycopeptide synthesis by using Si protecting group
川崎慶, 嶋脇健, 松下隆彦, 比能洋, 西村紳一郎, 日本化学会講演予稿集, 89th, 2, 2009

糖ペプチドチップを用いた糖ペプチド-タンパク質間相互作用の解析
松下隆彦, 越智里香, 五十嵐幸太, 比能洋, 西村紳一郎, 繊維学会予稿集, 64, 1, 2009

α-ジストログリカン糖ペプチドの効率的合成および機能解析
越智里香, 比能洋, 五十嵐幸太, 松下隆彦, 西村紳一郎, 日本薬学会年会要旨集, 129th, 2, 2009

ケイ素保護体アミノ酸ビルディングブロックを用いた糖ペプチド固相合成
川崎慶, 嶋脇健, 比能洋, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009

O-マンノース型糖ペプチドの効率的合成および機能解析
越智里香, 比能洋, 五十嵐幸太, 松下隆彦, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009

環状不凍糖タンパク質の合成および不凍活性
八須匡和, 高道学, 比能洋, 清水弘樹, 津田栄, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 2009

癌関連糖ペプチドライブラリーの構築と抗体エピトープマッピングへの応用
松下隆彦, 比能洋, 泉龍孝, 西村紳一郎, 大藪巨樹, 川本敬子, 沼田義人, 十亀弘子, 武本浩, 近藤裕郷, メディシナルケミストリーシンポジウム講演要旨集, 28th, 2009

新規不凍糖タンパク質誘導体の合成と性質
八須匡和, 比能洋, 高道学, 三浦信明, 清水弘樹, 津田栄, 西村紳一郎, 西村紳一郎, 日本糖質学会年会要旨集, 29th, 2009

Mechanism-Based Probing, Characterization, and Inhibitor Design of Glycosidases and Glycosyltransferases
Hiroshi Hinou, Shin-Ichiro Nishimura, CURRENT TOPICS IN MEDICINAL CHEMISTRY, 9, 1, 106, 116, Jan. 2009
English, Book review

WS-3-6 血清中糖鎖の網羅的解析による肝細胞癌新規バイオマーカーの開発(第108回日本外科学会定期学術集会)
横尾 英樹, 三浦 嘉晃, 神山 俊哉, 中西 一彰, 濱口 純, 工藤 岳秋, 古川 潤一, 中川 隆公, 田原 宗徳, 中馬 誠, 髭 修平, 篠原 康郎, 尾崎 倫孝, 多田 光宏, 松下 通明, 浅香 正博, 西村 紳一郎, 藤堂 省, 日本外科学会雑誌, 109, 2, 25 Apr. 2008
一般社団法人日本外科学会, Japanese

P-92 Study of Non-thermal Microwave Effects for Syntheses of Oligosaccharides and Oligopeptides(Poster Presentation)
Shimizu Hiroki, Matsushita Takahiko, Nagashima Izuru, Yoshimura Yayoi, Hiruma Shimizu Kazumi, Nishimura Shin-Ichiro, Symposium on the Chemistry of Natural Products, symposium papers, 50, 0, 813, 818, 2008
Although microwave irradiation has been used in chemical reactions as an efficient heating tool since 1986, we have found that microwave also contribute other factors while studying carbohydrate-related syntheses. 1) With microwave irradiation under Lewis acid conditions, methyl glycosides could behave as glycosyl donors. 2) In a case of synthesis Lewis X, microwave irradiation even at low temperatures was very effective as reducing byproducts and side-reactions. Especially, galactosylation for 4-OH in a fucosyl glucosamine acceptor has proceeded in high yield without producing byproducts, e.g., cleavage of the fucosyl bond or nucleophilic rearrangement of methylthio group. 3) Based on a preliminary study of microwave effect for coupling Fmoc-threonine having acetylated-Core2 to alanine onto supported resin, we successfully synthesized oligopeptide which consisted of twenty of amino acid residues with five of trisaccharides, a partial structure of MUC1 glycoprotein, in shorter time and higher yields. In addition, we also revealed that benzyl protection of glutamic acid was not suitable for basic microwave condition. 4) Microwave could activate azido groups and resulted to accelerate reductions by PPh_3 and Staudinger Ligations., Symposium on the Chemistry of Natural Products Steering Committee, Japanese

シアリダーゼ自殺基質におけるアグリコンフォーカスドライブラリーの作製と評価
甲斐宏一, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 2008

多糖類誘導体の整形外科領域での高次利用
比能洋, 西村紳一郎, 岩崎倫政, 三浪明男, 繊維学会予稿集, 63, 1/2, 2008

酵素の動的構造に着目したシアリダーゼ阻害剤の開発研究
高須康明, 黒河内政樹, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 2008

フォーカスドライブラリを用いた特異的シアル酸転移酵素阻害剤の探索
細口健作, 古川潤一, 前田哲宏, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 2008

大規模高速糖鎖構造解析法の開発と応用
天野麻穂, 比能洋, 古川潤一, 篠原康郎, 西村紳一郎, 日本化学会講演予稿集, 88th, 2, 2008

O-マンノース型糖ペプチドの効率的合成および機能解析に関する研究
越智里香, 比能洋, 五十嵐幸太, 西村紳一郎, 日本化学会講演予稿集, 88th, 2, 2008

BlotGlyco (R) and glycoblotting for large scale, high throughput glycomics
Yoshiaki Miura, Shin-Ichiro Nishimura, TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, 20, 111, 17, 27, Jan. 2008
English, Book review

Microwave Chemistry for Glycosylation and Oligopeptide Synthesis
SHIMIZU Hiroki, MATSUSHITA Takahiko, NISHIMURA Shin-Ichiro, 高分子論文集, 64, 12, 883, 896, 25 Dec. 2007
高分子学会, Japanese

Glycochemical biology
長堀 紀子, 西村 紳一郎, Protein, nucleic acid and enzyme, 52, 13, 1742, 1750, Oct. 2007
共立出版, Japanese

TAK1 represses transcription of the human telomerase reverse transcriptase gene
T. Fujiki, T. Miura, M. Maura, H. Shiraishi, S. Nishimura, Y. Imada, N. Uehara, K. Tashiro, S. Shirahata, Y. Katakura, ONCOGENE, 26, 36, 5258, 5266, Aug. 2007
English

糖鎖研究からバイオ産業育成への挑戦
西村 紳一郎, Journal of applied glycoscience, 54, 20 Jul. 2007
Japanese

高速化が進む 糖鎖解析--糖鎖構造研究を加速する新しい解析法:グライコブロッティング法
天野 麻穂, 西村 紳一郎, 現代化学, 435, 55, 61, Jun. 2007
東京化学同人, Japanese

腫瘍マーカーに対する糖鎖修飾が誘導する立体構造変化
藤谷直樹, 黒河内政樹, GAO Xiao-Dong, 松下隆彦, 比能洋, 篠原康郎, 西村紳一郎, Abstracts. Annual Meeting of the NMR Society of Japan, 46th, 2007

ポリラクトサミン含有複合糖質のライブラリー構築と構造解析
成地健太郎, 浜本智樹, 黒河内政樹, 松下隆彦, 藤谷直樹, 比能洋, 比能洋, 西村紳一郎, 西村紳一郎, 高分子学会北海道支部研究発表会講演要旨集, 41st, 2007

平成18年度第37回繊維学会夏季セミナー
西村 紳一郎, 繊維学会誌, 62, 12, "P, 366"-"P-370", 10 Dec. 2006
社団法人 繊維学会, Japanese

Construction of tumor - Related gkycpeptided library
Shin Ichiro Nishimura, Hiroshi Hinou, Hiroki Shimizu, Takahiko Matsushita, Naoki Oyabu, Masataka Fumoto, Hirosato Kondo, Polymer Preprints, Japan, 55, 5508, 01 Dec. 2006

Identification of N-glycans related with cartilage deterioration
Tomoya Matsuhashi, Norimasa Iwasaki, Hiroaki Nakagawa, Megumi Hato, Masaki Kurogochi, Kisaburo Deguchi, Tokifumi Majima, Akio Minami, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1152, 1153, Nov. 2006
English, Summary international conference

A glycomic approach to drug resistance: Direct interaction between Cisplatin and N-glycans
Hiroaki Nakagawa, Shinji Hayashi, Shigeaki Abe, Noriko Nagahori, Kenji Monde, Miki Ichikawa-Ohira, Hirosato Kondo, Shin-Ichi Akiyama, Kisaburo Deguchi, Akira Nakagawara, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1150, 1150, Nov. 2006
English, Summary international conference

All-in-one processing of oligosaccharides on solid-support (A1POS); A general protocol with Glycoblotting for functional glycomics (part 1)
Yoshiaki Miura, Jun-ichi Furukawa, Yasuro Shinohara, Hiromitsu Kuramoto, Masaki Kurogochi, Hideyuki Shimaoka, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1135, 1135, Nov. 2006
English, Summary international conference

An efficient, rapid, differential N-glycan profiling from mouse tissues
Masaki Kurogochi, Yasuro Shinohara, Yoshiaki Miura, Jun-Ichi Furukawa, Hideyuki Shimaoka, Hiromitsu Kuramoto, Yoko Kita, Mika Nakano, Hiroki Ito, Hiroaki Nakagawa, Kisaburo Deguchi, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1137, 1137, Nov. 2006
English, Summary international conference

Alterations in N-glycans seen in drug-resistant human hepatocellular carcinoma
Takeaki Kudo, Hiroaki Nakagawa, Masato Takahashi, Jun Hamaguchi, Naoya Kamiyama, Hideki Yokoo, Kazuaki Nakanishi, Takahito Nakagawa, Toshiya Kamiyama, Kisaburo Deguchi, Satoru Todo, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1151, 1151, Nov. 2006
English, Summary international conference

Increased alpha 1,6-fucosylation of N-glycan in serum glycoprotein of db/db mice
Naofumi Itoh, Shinji Sakaue, Hiroaki Nakagawa, Masaki Kurogochi, Kisaburo Deguchi, Shin-Ichiro Nishimura, Masaharu Nishimura, GLYCOBIOLOGY, 16, 11, 1152, 1152, Nov. 2006
English, Summary international conference

Combined use of hydrazide functionalized polymer and sequential tag exchange; A general protocol with Glycoblotting for functional glycomics (part 2)
Jun-ichi Furukawa, Yoshiaki Miura, Hiromitsu Kuramoto, Hideyuki Shimaoka, Masaki Kurogochi, Mika Nakano, Yasuro Shinohara, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1139, 1139, Nov. 2006
English, Summary international conference

Unusual N-glycans from alpha-mannosidase II/IIx double knockout mice identified by a systematic glycomic approach using MDSF method in MALDI-TOF/TOF-MS
Megumi Hato, Hiroaki Nakagawa, Masaki Kurogochi, Kisaburo Deguchi, Tomoya O. Akama, Jamey D. Marth, Michiko N. Fukuda, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1124, 1124, Nov. 2006
English, Summary international conference

GnT-V expression correlates with patient survival in bladder cancer
Shingo Hatakeyama, Hirofumi Ishimura, Toshiko Takahashi, Hiroaki Nakagawa, Shin-Ichiro Nishimura, Yohei Horikawa, Eiji Miyoshi, Atsushi Kyan, Shigeru Hagisawa, Tomonori Habuchi, Yoichi Arai, Chikara Ohyama, GLYCOBIOLOGY, 16, 11, 1151, 1152, Nov. 2006
English, Summary international conference

Novel chiroptical analysis of glycoconjugates by Vibrational Circular Dichroism (VCD)
Kenji Monde, Tohru Taniguchi, Masami Fukuzawa, Mai Hashimoto, Atsufumi Nakahashi, Nobuaki Miura, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1136, 1137, Nov. 2006
English, Summary international conference

N-glycans correlate 5-FU resistance in colon 26 and derived cell lines
Jun Hamaguchi, Hiroaki Nakagawa, Masato Takahashi, Takeaki Kudo, Naoya Kamiyama, Bailong Sun, Yuji Sato, Kisaburo Deguchi, Satoru Todo, Shin-Ichiro Nishimura, GLYCOBIOLOGY, 16, 11, 1151, 1151, Nov. 2006
English, Summary international conference

Efficient syntheses and characterizations of MUC1 related high-molecular weight glycopeptides
Takahiko Matsushita, Toshiki Ohyabu, Toshiki Ohyabu, Hiroshi Hinou, Hiroki Shimizu, Shin Ichiro Nishimura, Shin Ichiro Nishimura, Polymer Preprints, Japan, 55, 1857, 19 Oct. 2006

トリスキレート型金属錯体の光学分割とVCD
SATO HISAKO, TANIGUCHI TOORU, MONDE KENJI, NISHIMURA SHIN'ICHIRO, YAMAGISHI AKIHIKO, 錯体化学討論会講演要旨集, 56th, 326, 08 Sep. 2006
Japanese

トリスおよびビスアセチルアセトナト金属錯体のVCD測定と理論計算
SATO HISAKO, TANIGUCHI TOORU, MONDE KENJI, NISHIMURA SHIN'ICHIRO, YAMAGISHI AKIHIKO, 錯体化学討論会講演要旨集, 56th, 34, 08 Sep. 2006
Japanese

CARB 63-One-pot solid phase glycoblotting and labeling by trans-oximization for high-throughput glycomics and glycoproteomics
Hideyuki Shimaoka, Jun-ichi Furukawa, Yoshiaki Miura, Masaki Kurogochi, Hiroshi Hinou, Yasuro Shinohara, Kisaburo Deguchi, Shin-Ichiro Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 232, Sep. 2006
English, Summary international conference

Change your conception at Hokkaido! Crisis of earth-warming can be considered to be a new business chance.
Shinichiro Nishimura, SEN-I GAKKAISHI, 62, 8, P231, P231, Aug. 2006
Japanese, Others

糖鎖自動分析システムの医療への応用
西村 紳一郎, 臨床化学, 35, 1, 2, 14 Jul. 2006
Japanese

colon26における5FU耐性株のN型糖鎖の変化
濱口 純, 工藤 岳秋, 神山 直也, 孫 白龍, 高橋 将人, 佐藤 裕二, 出口 喜三郎, 中川 裕幸, 西村 紳一郎, 藤堂 省, 日本外科学会雑誌, 107, 2, 05 Mar. 2006
一般社団法人日本外科学会, Japanese

ダイナミックな作用機序に基づく糖転移酵素阻害剤の分子設計法
西村紳一郎, 西村紳一郎, 高谷健二, 前田哲宏, 細口健作, 古川潤一, 比能洋, 日本糖質学会年会要旨集, 26th, 2006

癌関連糖ペプチドライブラリの構築
西村紳一郎, 比能洋, 清水弘樹, 松下隆彦, 大藪巨樹, 麓昌高, 近藤裕郷, 高分子学会予稿集(CD-ROM), 55, 2 Disk1, 2006

MUC1関連高分子糖ペプチドの効率的合成と機能評価
松下隆彦, 大藪巨樹, 大藪巨樹, 比能洋, 清水弘樹, 西村紳一郎, 西村紳一郎, 高分子学会予稿集(CD-ROM), 55, 1 Disk1, 2006

酵素連続反応による糖鎖自動合成法 糖鎖自動合成装置Golgiの開発
西村紳一郎, 清水弘樹, 比能洋, 産総研TODAY, 6, 2, 2006

自殺基質によるシアリダーゼ解析
比能洋, 黒河内政樹, 秋場聡, 武川泰啓, 西村紳一郎, 日本糖質学会年会要旨集, 26th, 2006

質量分析法による糖鎖構造解析
出口 喜三郎, 西村 紳一郎, 化学と工業 = Chemistry and chemical industry, 58, 12, 1422, 1425, 01 Dec. 2005
日本化学会, Japanese

Modification of Chitosan with Bioactive Oligosaccharide
LI Xuebing, WATANABE Shinya, SUZUKI Takashi, SUZUKI Yasuo, WATANABE Miho, NATORI Yasuhiro, NISHIKAWA Kiyotaka, KITA Eiji, MONDE Kenji, NISHIMURA Shin-Ichiro, キチン・キトサン研究 = Chitin and chitosan research, 11, 2, 120, 121, 01 Jul. 2005
Japanese

合成不凍糖タンパクを用いた膵島凍結保存法の開発(第105回日本外科学会定期学術集会)
松本 秀一朗, 松下 通明, 築山 周作, 森田 恒彦, 蒲池 浩文, 古川 義純, 西村 紳一郎, 藤堂 省, 日本外科学会雑誌, 106, 0, 05 Apr. 2005
一般社団法人日本外科学会, Japanese

colon26における5FU耐性株のN型糖鎖の変化(第105回日本外科学会定期学術集会)
濱口 純, 中川 裕章, 工藤 岳秋, 高橋 将人, 佐藤 裕二, 出口 喜三郎, 中川原 章, 西村 紳一郎, 藤堂 省, 日本外科学会雑誌, 106, 0, 05 Apr. 2005
一般社団法人日本外科学会, Japanese

不凍糖タンパク質(AFGP)不凍活性メカニズムの解明
八須匡和, 五十嵐幸太, 津田栄, 越田周平, 門出健次, 西村紳一郎, 高分子学会予稿集(CD-ROM), 54, 1 Disk1, 2005

糖鎖改変技術の創薬応用への可能性(話題)
西村 紳一郎, ファルマシア, 41, 4, 325, 330, 2005
公益社団法人 日本薬学会, Japanese

Progress and Future of Carbohydrate Sequencing-Toward Glycoform-focused reverse genomics-
Nishimura Shin-Ichiro, Japanese Journal of Clinical Chemistry, 34, 4, 297, 308, 2005
Japan Society of Clinical Chemistry, Japanese

Progress and Future of Carbohydrate Sequencing –Toward Glycoform-focused reverse genomics–
Shin-Ichiro Nishimura, Japanese Journal of Clinical Chemistry, 34, 4, 297, 308, 2005
English

糖鎖自動合成装置“Golgi”用糖転移酵素の開発と利用
岩田一道, 太田尚志, 太田尚志, 山田久里子, 山田久里子, 戸田篤志, 戸田篤志, 比能洋, 西村紳一郎, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 23rd, 2005

Development and application of the water-soluble primer for rapid and efficient glycopeptides synthesis
麓昌高, 比能洋, 太田尚志, 伊藤孝臣, 瀧本明生, 近藤裕郷, 稲津敏行, 中原義昭, 西村紳一郎, 日本化学会講演予稿集, 85th, 2, 2005

Development of a Mechanism-based Inhibitor of Neuraminidases
比能洋, 黒河内政樹, 長堀紀子, 西村紳一郎, 日本化学会講演予稿集, 85th, 2, 2005

Solid-phase glycopeptide synthesis under Microwave irradiation
松下隆彦, 比能洋, 清水弘樹, 西村紳一郎, 日本化学会講演予稿集, 85th, 2, 2005

糖鎖自動合成装置“Golgi”の開発
山田久里子, 山田久里子, 太田尚志, 太田尚志, 岩田一道, 麓昌高, 麓昌高, 大藪巨樹, 大藪巨樹, 比能洋, 清水弘樹, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 23rd, 2005

糖ペプチド合成用プライマーの開発と糖ペプチドライブラリーへの応用
麓昌高, 麓昌高, 比能洋, 比能洋, 清水弘樹, 太田尚志, 太田尚志, 松下隆彦, 岩田一道, 山田久里子, 山田久里子, 伊藤孝臣, 大藪巨樹, 大藪巨樹, 瀧本明生, 近藤裕郷, 稲津敏行, 中原義昭, 西村紳一郎, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 23rd, 2005

Functional glycoclusters having chitosan skeleton
LI Xuebing, WATANABE Shinya, SUZUKI Takashi, SUZUKI Yasuo, NATORI Yasuhiro, NISHIKAWA Kiyotaka, MONDE Kenji, NISHIMURA Shin-Ichiro, キチン・キトサン研究 = Chitin and chitosan research, 10, 2, 204, 205, 01 Jul. 2004
Japanese

インタビュー 与えられたチャンスを活かせ! 大学院で何を学び,何をスキルアップさせるか (特集 新大学院時代の成功プログラム)
西村 紳一郎, 化学, 59, 4, 30, 32, Apr. 2004
化学同人, Japanese

Development of Mechanism-Based Fluorescence Affinity Labeling Reagent for β-Galactosidase as Proteomics Tool : A Detailed Analysis Using MALDI-TOF Mass Spectrometry
KUROGOCHI Masaki, IWATA Kazumichi, MONDE Kenji, NIIKURA Kenichi, LEE Yuan Chuan, NISHIMURA Shin-Ichiro, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2003, 113, 114, 01 Mar. 2004
English

Conformational anlyses of carbohydrates by the molecular orbital theory and VCD spectra
Miura Nobuaki, Taniguchi Tohru, Monde Kenji, Nishimura Shin-Ichiro, Abstracts of Symposium on Physical Organic Chemistry, 17, 0, 51, 51, 2004
糖鎖は不斉炭素の集合体であり、その配座解析の確固たる方法論が未だにない。我々は配座解析の強力なツールとして分子軌道法を用い、不斉性を検出可能な振動領域円二色性スペクトルを計算することによって、測定されたスペクトルを再現することによって糖鎖の配座を混合比のレベルでの解析を行う。, The Society of Physical Organic Chemistry, Japan

糖鎖自動合成装置“Golgi”による複合糖質の合成
山田久里子, 戸田篤志, 西口進, 岩田一道, 麓昌高, 比能洋, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 22nd, 2004

糖鎖自動合成装置“Golgi”用固定化糖転移酵素の開発
戸田篤志, 山田久里子, 比能洋, 西村紳一郎, バイオテクノロジーシンポジウム予稿集, 22nd, 2004

N-glycan patterns of human serum in rheumatoid arthritis
M Hato, H Nakagawa, Y Takegawa, K Deguchi, K Monde, N Iwasaki, A Minami, H Kondo, SI Nishimura, GLYCOBIOLOGY, 13, 11, 870, 870, Nov. 2003
English, Summary international conference

Structural assignment of isomeric 2-aminopyridine derivatized oligosaccharides using Ms-n spectral matching
Y Takegawa, S Ito, S Yoshioka, K Deguchi, H Nakagawa, K Monde, SI Nishimura, GLYCOBIOLOGY, 13, 11, 844, 845, Nov. 2003
English, Summary international conference

Structures of N-glycan from human dermis
R Uematsu, H Nakagawa, K Deguchi, M Ota, K Yokota, H Shimizu, K Monde, SI Nishimura, GLYCOBIOLOGY, 13, 11, 846, 846, Nov. 2003
English, Summary international conference

Glycoblotting: Synthetic nanoparticles for rapid isolation and analysis of glycopatterns
SI Nishimura, K Niikura, N Nagahori, R Uematsu, H Nakagawa, S Abe, H Moriyama, R Sadamoto, K Deguchi, K Monde, H Kondo, GLYCOBIOLOGY, 13, 11, 843, 844, Nov. 2003
English, Summary international conference

次世代創薬のための糖鎖工学 (特集 革新的医薬品開発のための糖鎖科学)
新倉 謙一, 西村 紳一郎, 化学工業, 54, 10, 745, 747, Oct. 2003
化学工業社, Japanese

Synthesis and characterization of functional glycolipid LB membrane.
N Nagahori, R Sadamoto, K Niikura, K Monde, SI Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 226, U200, U200, Sep. 2003
English, Summary international conference

Stereochemical chiral analysis of carbohydrates by vibrational circular dichroism.
K Monde, T Taniguchi, N Miura, SI Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 226, U213, U213, Sep. 2003
English, Summary international conference

生合成プロセスを模倣した糖鎖自動合成装置"Golgi" (糖鎖機能--第3の生命鎖) -- (構造生物学・新たな構造機能解析法)
西村 紳一郎, 蛋白質核酸酵素, 48, 8, 1220, 1225, Jun. 2003
共立出版, Japanese

糖鎖工学が拓く新しい医薬開発
新倉 謙一, 西村 紳一郎, 化学と工業 = Chemistry and chemical industry, 56, 3, 223, 224, 01 Mar. 2003
Japanese

糖鎖自動合成装置の開発 (特集 糖鎖工学の最前線)
西村 紳一郎, バイオインダストリ-, 20, 1, 44, 49, Jan. 2003
シ-エムシ-, Japanese

糖鎖エンジニアリングプロジェクト/糖鎖構造解析技術開発 糖鎖自動合成装置のさらなる改良に向けて
西村紳一郎, 比能洋, バイオテクノロジーシンポジウム予稿集, 21st, 2003

解説 基礎研究から応用までのキーテクノロジー 糖類自動合成装置"Golgi"
西村 紳一郎, 化学, 57, 8, 18, 21, Aug. 2002
化学同人, Japanese

Development of automated glycosynthesizer
NISHIMURA Shin-ichiro, Bioscience & industry, 60, 8, 23, 26, 01 Aug. 2002
バイオインダストリ-協会, Japanese

Chemical modification of the living bacterial cell wall.
R Sadamoto, SI Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 223, U100, U100, Apr. 2002
English, Summary international conference

機能化を目指した糖脂質クラスターの構築 (マイクロドメイン形成と細胞のシグナリング--スフィンゴ脂質の新しい生物機能) -- (第5部 スフィンゴ脂質プローブの開発と応用)
新倉 謙一, 西村 紳一郎, 蛋白質核酸酵素, 47, 4, 543, 550,292, Mar. 2002
共立出版, Japanese

解説記事 糖鎖クラスターの構築と機能
西村 紳一郎, 未来材料, 2, 2, 26, 31, Feb. 2002
エヌ・ティー・エス, Japanese

Protective mechanism of reduced water against alloxan-induced pancreatic beta-cell damage: Scavenging effect against reactive oxygen species
YP Li, T Nishimura, K Teruya, T Maki, T Komatsu, T Hamasaki, T Kashiwagi, S Kabayama, SY Shim, Y Katakura, K Osada, T Kawahara, K Otsubo, S Morisawa, Y Ishii, Z Gadek, S Shirahata, CYTOTECHNOLOGY, 40, 1-3, 139, 149, 2002
English

21 Synthetic studies on glycopeptide polymers having ice crystal growth inhibition activity
Tachibana Yuki, Igarashi Kota, Monde Kenji, Nishimura Shin-Ichiro, Tsuda Sakae, Symposium on the Chemistry of Natural Products, symposium papers, 44, 0, 121, 126, 2002
Antifreeze glycoproteins [AFGPs: {Ala-Thr(Galβ1-3GalNAcα)-Ala}_n, n=4-50] in the serum of polar fishes have been known to depress the freezing point of their blood and help them to survive at the temperature below-1.9℃[1]. Though it is widely accepted that AFGPs bind to the ice surface and prevent its growth, the definitive molecular mechanism of the action has not been solved yet. In this study, structure-activity relationships in AFGPs were studied toward understanding of the mechanism, thorough chemical syntheses, activity evaluations, and conformational analyses of the AFGP and its analogues, in which sugar or peptide moieties were different from those of the wild-type AFGP. The AFGP (1) and its analogues (2-8) were successfully synthesized via simple polymerization of their repeating unit using a DPPA method without any protection of sugar -OH groups [2]. Additionally, sialyl T-bearing glycopolypeptide (9) was successfully prepared through enzymatic elongation of a sialic acid residue to C-3 position of the galactose residue in the AFGP (1), indicating the broad applicability of this methodology for the syntheses of various glycopeptide polymers. In the evaluation assay of the antifreeze activity, artificial AFGP (1) showed similar activity as that of the wild-type AFGP. On the contrary, neither an AFGP repeating unit glycopeptide (19) nor a sugar-lacked polypeptide (poly-ATA, 8) shows the activity. Interestingly, AFGP analogs having GalNAc (2) or LacNAc (3) as sugar moiety showed similar activities as AFGP, while that of Galactose (4), Lactose (5), β-O-linked (6), and ASA (7) did not. These results clearly indicate the importance of i) repeating structure of glycopeptide, ii) NHAc group at C-2 position of the sugar moiety directly attached to the peptide, iii) α-glycosidic linkage between the sugar and the threonyl residue, for the specific interaction with ice. Furthermore, secondary structural analyses of the synthetic compounds were carried out by means of a CD spectroscopic method, in order to clarify the relationships between their conformations and their activities. The CD spectra of active compounds (1-3) were obviously different from those of inactive analogues (4-8), suggesting the presence of an ordered structure, but not α-helix., Symposium on the Chemistry of Natural Products Steering Committee, Japanese

糖鎖の自動合成に挑戦(「バイオ」分子を作る化学)
西村 紳一郎, 化学と教育, 50, 3, 181, 183, 2002
次世代ポストゲノム研究の重要な柱となることが予想される糖鎖工学の基盤技術の一つは, 糖鎖をいかにスピーディーに確実に合成するかであろう。我々は酵素(糖転移酵素)の反応を基本とする自動合成法の開発を目指して研究を進めてきた。有機溶剤・保護基などを用いない環境低負荷型の合成プロセスとしても評価されつつある。, 公益社団法人 日本化学会, Japanese

Combinatorial syntheses of sugar derivatives
Shin-Ichiro Nishimura, Current Opinion in Chemical Biology, 5, 3, 325, 335, 01 Jun. 2001
Elsevier Ltd, English, Book review

Protein patterning on thin film.
R Sadamoto, K Niikura, N Nagahori, K Yamada, SI Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 221, U351, U351, Apr. 2001
English, Summary international conference

生合成を模倣した糖鎖自動合成法の構築
西村 紳一郎, 日本農芸化学会誌, 75, 05 Mar. 2001
English

The critical role of Th1-dominant immunity in tumor′ immunology.
Nishimura T., Nakui M., Sato M., Iwakabe K., Kitamura ′ H., Sekimoto M., Ohta A., Koda T., Nishimura S., Collected papers from Institute for Genetic Medicine Hokkaido University, 2, 418, 426, 2001
Hokkaido University, English

Expression of β1,3-N-acetylglucosaminyltransferase from Streptococcus agalactiae type Ia in Escherichia coli as a fusion with maltose-binding protein and synthesis of oligosaccharide using a fusion protein.
Toda Atsushi, Shibatani Shigeo, Nishiguchi Susumu, Nakagawa Hiroaki, Yamada Kuriko, Kurokouchi Masaki, Nishimura Shin-Ichiro, 日本生物工学会大会講演要旨集, 13, 337, 337, 2001
日本生物工学会, Japanese

Expression of mouse GD3 synthase in E.coli as a fusion protein with maltose binding protein.
Chiyo Naoki, Toda Atsushi, Shibatani Shigeo, Nishiguchi Susumu, Nishimura SHin-ichiro, 日本生物工学会大会講演要旨集, 13, 338, 338, 2001
日本生物工学会, Japanese

糖鎖自動合成への挑戦--水溶性高分子への糖転移反応を利用する (糖鎖分子の設計と生理機能) -- (酵素を用いた糖鎖合成)
西村 紳一郎, 季刊化学総説, 48, 3, 9, 2001
学会出版センタ-, Japanese

Controlling the topology of the biomembrane model by modulating the surface protein.
N Nagahori, K Nikura, K Yamada, SI Nishimura, GLYCOBIOLOGY, 10, 10, 1120, 1120, Oct. 2000
English, Summary international conference

Biomimetic strategy of glycoconjugates synthesis: Combined use of immobilized enzymes and designed primers
SI Nishimura, K Yamada, M Kurogochi, S Nishiguchi, S Shibatani, A Toda, H Nakagawa, GLYCOBIOLOGY, 10, 10, 1100, 1100, Oct. 2000
English, Summary international conference

多孔性ポリマーフィルムを利用した肝細胞の形態及び機能制御　　　　　　　　　　　　　　　
西川 和孝, 西川 雄大, 西田 仁, 大倉 隆介, 西村 紳一郎, 下村 政嗣, 斉藤 孝則, 藤本 哲也, 大久保 尚, 蒲池 浩文, 北海道外科雑誌, 45, 1, 90, 90, Jun. 2000
北海道外科学会, Japanese

Mixed Spinning of Chitosan Oligomer and Alginate
TAMURA Hiroshi, TSURUTA Yukihiko, TOKURA Seiichi, NISHIMURA Shin-ichiro, キチン・キトサン研究 = Chitin and chitosan research, 6, 2, 102, 103, 01 May 2000
Japanese

Design of Functional Chito-oligosaccharide Hybridpolymers
NONAKA Sachiko, KUMAGAI Daiju, MAEKAWA Yoshihiko, OTA Takashi, NISHIMURA Shin-Ichiro, キチン・キトサン研究 = Chitin and chitosan research, 6, 2, 106, 107, 01 May 2000
Japanese

複合糖質科学の研究拠点を設立--自然環境・産業構造にマッチした新バイオサイエンスエリアへの展望 (寒木春華のくに……北海道)
西村 紳一郎, 季刊河川レビュ-, 29, 4, 8, 13, 2000
新公論社, Japanese

Controlling the topology of glyco-ligand in a biological model system
N Nagahori, M Taniguchi, A Yamagishi, SI Nishimura, GLYCOBIOLOGY, 9, 10, 1116, 1116, Oct. 1999
English, Summary international conference

Fundamental studies on the automated glycoconjugates synthesis
YAMADA Kuriko, NISHIMURA Shin-ichiro, バイオサイエンスとインダストリー = Bioscience & industry, 57, 9, 35, 36, 01 Sep. 1999
Japanese

Antifreeze Proteins
TSUDA Tetsuro, MATSUBARA Naoki, NISHIMURA Shinichiro, Kobunshi, 48, 5, 343, 348, May 1999
The Society of Polymer Science, Japan, Japanese

不凍糖タンパク質の合成--不凍魚の秘密をさぐる
西村 紳一郎, 現代化学, 337, 56, 62, Apr. 1999
東京化学同人, Japanese

Syntheses of polysaccharide chains by using polymer supports
K Yamada, SI Nishimura, SEN-I GAKKAISHI, 54, 9, P318, P322, Sep. 1998
Japanese, Others

Preparation and Properties of Chitosan and Chitin Having Mannoside Branches
KURITA K., KOBAYASHI M., SHIMADA K., MORI T., ISHII S., NISHIMURA S., NISHIYAMA Y., SHIMOJOH M., キチン・キトサン研究 = Chitin and chitosan research, 4, 2, 220, 221, 15 May 1998
Japanese

Polymer-assisted enzymatic synthesis of neosphingoglycolipids.
K Yamada, SI Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 215, U117, U117, Apr. 1998
English, Summary international conference

Chemo-enzymatic synthesis of new glycoconjugate polymers bearing sialyl Lewis(X) pendant chains.
F Sallas, SI Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 215, U105, U105, Apr. 1998
English, Summary international conference

Synthetic studies on specific glyco-ligands by molecular imprinting method
N Nagahori, K Yamada, SI Nishimura, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 215, U117, U117, Apr. 1998
English, Summary international conference

Design of Functional Supramolecule Based on Chitin and Chitosan
NISHIDA Jin, NISHIMURA Shin-Ichiro, IJIRO Kuniharu, OLAF Karthous, SHIMOMURA Masatsugu, キチン・キトサン研究 = Chitin and chitosan research, 3, 2, 112, 113, 15 May 1997
Japanese

Synthesis of Glycoconjugates
NISHIMURA Shinichiro, Kobunshi, 45, 8, 539, 543, 01 Aug. 1996
The Society of Polymer Science, Japan, Japanese

糖転移酵素の新しい活性測定法
富士 祥聡, 西村 紳一郎, 日本分子生物学会年会プログラム・講演要旨集, 19, 393, 393, 01 Aug. 1996
Japanese

RGDS含む人工タンパク質の合成とその生物活性
松田 匡雄, 西村 紳一郎, 日本分子生物学会年会プログラム・講演要旨集, 19, 393, 393, 01 Aug. 1996
Japanese

Selective Synthesis of Glycoconjugate Polymers Using Enzymatic Reactions
NISHIMURA Shin-Ichiro, 高分子学会予稿集, 45, 1, 16, 18, 10 May 1996
Japanese

Molecular Recognition and Carbohydrates
NISHIMURA Shin-Ichiro, キチン・キトサン研究 = Chitin and chitosan research, 2, 2, 86, 87, 01 May 1996
Japanese

BLOOD COAGULATION EFFECT OF CHITIN PHOSPHATE
TSUJI Youji, YOSHIDA Takashi, NISHIMURA Shin-Ichiro, SAKAIRI Nobuo, NISHI Norio, TOKURA Seiichi, キチン・キトサン研究 = Chitin and chitosan research, 2, 2, 114, 115, 01 May 1996
Japanese

グリコシル化反応
西村 紳一郎, 高分子, 45, 8, 563a, 563a, 1996
公益社団法人 高分子学会, Japanese

糖供与体・糖受容体
西村 紳一郎, 高分子, 45, 8, 563b, 563b, 1996
公益社団法人 高分子学会, Japanese

Biological activities of biodegradable polysaccharide
S Tokura, SI Nishimura, N Sakairi, N Nishi, MACROMOLECULAR SYMPOSIA, 101, 389, 396, Jan. 1996
English

Synthesis of Antifreeze Glycoprotein
TSUDA Tetsuro, NISHIMURA Shin-Ichiro, Peptide chemistry : proceedings of the ... Symposium on Peptide Chemistry, 1995, 145, 148, 01 Oct. 1995
English

Structure-Function Relationship of Molecular Chaperone Protein -Nucleoplasmin-
HOZUMI Kentaro, IWATA Kazumichi, FUKUE Keiji, ITOH Toru, SAKAIRI Nobuo, NISHIMURA Shin-Ichiro, TOKURA Seiichi, KATAGIRI Chiaki, NISHI Norio, Peptide Chemistry 1995, 1995, 341, 344, 01 Oct. 1995
English

Synthesis of Novel Clustered Glycopolymers Containing Triantennary Glycosides of N-Acetyllactosamine^1
FURUIKE Tetsuya, NISHI Norio, TOKURA Seiichi, NISHIMURA Shin-ichiro, Chemistry letters, 1995, 9, 823, 824, 05 Sep. 1995
English

The Application of Regenerated Chitin for Drug Delivery System
TOKURA Seiichi, TAKAHASHI Kiyohisa, SHIRAI Akihiro, SAKAIRI Nobuo, NISHI Norio, NISHIMURA Shin-Ichiro, キチン・キトサン研究 = Chitin and chitosan research, 1, 2, 72, 73, 15 Apr. 1995
Japanese

Synthesis of Branched Chitin Derivartives
ISHII Shigeru, KOBAYASHI Masayuki, AKAO Hirofumi, KURITA Keisuke, NISHIMURA Shin-Ichiro, キチン・キトサン研究 = Chitin and chitosan research, 1, 2, 162, 163, 15 Apr. 1995
Japanese

Chitin and Chitosan as Fine Polysaccharide Resources
NISHIMURA SHIN-ICHIRO, キチン・キトサン研究 = Chitin and chitosan research, 1, 1, 15, 25, 30 Jan. 1995
Japanese

SELECTIVE SULFATION OF CHITIN DERIVATIVES FOR BIOMEDICAL FUNCTIONS
S TOKURA, K ITOYAMA, N NISHI, SI NISHIMURA, SAIKI, I, AZUMA, I, JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, A31, 11, 1701, 1718, 1994
English

Glycobiology and Glycoconjugate Models
Shin-ICHIRO Nishimura, Sen'i Gakkaishi, 48, 4, P158, P164, 1992
The Society of Fiber Science and Technology, Japan, English

朝鮮人参に含まれる生理活性多糖パナキサンAへの合成化学的アプローチ(有機化学・天然物化学-反応と合成(糖質)-)
市川 幸孝, 西村 紳一郎, 坂入 信夫, 葛原 弘美, 瓜生 敏之, 日本農藝化學會誌, 62, 3, 15 Mar. 1988
社団法人日本農芸化学会, Japanese

Preparation and Some Properties of Variously Deacetylated Chitin Fibers
Seiichi Tokura, Shin-Ichiro Nishimura, Norio Nishi, Kunio Nakamura, Osatoshi Hasegawa, Hitoshi Sashiwa, Hiroshi Seo, Sen'i Gakkaishi, 43, 6, 288, 293, 1987
The Society of Fiber Science and Technology, Japan, English

非哺乳類多糖の新しい展開--新素材としてのキチンの医学領域での利用を中心に
戸倉 清一, 西村 紳一郎, 蛋白質核酸酵素, 31, 15, p1621, 1631, Dec. 1986
共立出版, Japanese

Lysozyme-accessible fibers from chitin and its derivatives
Seiichi Tokura, Norio Nishi, Shin Ichiro Nishimura, Oyin Somorin, Sen'i Gakkaishi, 39, 12, T507, T511, 1983
The Society of Fiber Science and Technology, Japan, English

〔Major achievements〕Glycoscience 2nd Edition. 2115-2119　　　　　　　　　　　　　　　
Springer, 2008

〔Major achievements〕Glycoscience 2nd Edition. 1859-1871　　　　　　　　　　　　　　　
Springer, 2008

糖鎖のMALDI-TOFMSスペクトルデータブック (西村 紳一郎、福島信弘編)　　　　　　　　　　　　　　　
三共出版, 2007

〔Major achievements〕Comprehensive Glycoscience, 453-475　　　　　　　　　　　　　　　
Elsevier Ltd., 2007

糖鎖のMALDI-TOFMSスペクトルデータブック
黒河内 政樹, 広瀬 和子, 西村 紳一郎, 福島 信弘
三共出版, 2007, 9784782705278, Japanese

Nanotechnology in Carbohydrate Chemistry (Yuasa H.監), 149-166　　　　　　　　　　　　　　　
Transworld Research Network, 2006

Methods in Enzymology, 415, 202-212　　　　　　　　　　　　　　　
Academic Press, 2006

未来を拓く糖鎖科学 (永井克彦監), 419-420　　　　　　　　　　　　　　　
金芳堂, 2005

Handbook of Carbohydrate Engineering (Yarema K. 編), 495-505　　　　　　　　　　　　　　　
Taylor & Francis, 2005

〔Major achievements〕糖鎖生物学入門
Taylor Maureen, Drickamer Kurt, 西村 紳一郎, 門出 健次
化学同人, 2005, 9784759810356, Japanese

Methods in Enzymology, 362, 273-286　　　　　　　　　　　　　　　
Academic Press, 2003

Carbohydrate-based Drug Discovery, 129-136　　　　　　　　　　　　　　　
Wiley-VCH, DEU, 2003

〔Major achievements〕生命高分子科学入門 : 生物に学び生物を超える
西村 紳一郎, 畑中 研一, 佐藤 智典, 和田 健彦
講談社, 1999, 4061397915, Japanese

〔Major achievements〕糖質の科学と工学
畑中 研一, 西村 紳一郎, 大内 辰郎, 小林 一清, 講談社サイエンティフィク
講談社, 1997, 4061397834, Japanese

A theory of dynamic epitope -Unexplored drug targets elaborated by disease-specific posttranslational protein glycosylation-　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
2024 Hokkaido University – University of Massachusetts Amherst Virtual Symposium on Polymer Science and Engineering, 31 Jan. 2024, English, Invited oral presentation
[Invited]

糖ペプチドワクチン：逃避変化しない糖鎖修飾領域を標的とする革新的なワクチンモダリティに関する研究開発　　　　　　　　　　　　　　　
西村紳一郎
第2回 近未来ワクチンフォーラム, 23 Jan. 2024, Japanese
[Invited]

「革新的アジュバント・ワクチンキャリアの開発と技術支援ならびにデータベースの構築―キャリア開発と評価（ナノソーム）―　　　　　　　　　　　　　　　
西村紳一郎
AMED SCARDA ワクチン・新規モダリティ研究開発事業「革新的アジュバント・ワクチンキャリアの開発と技術支援ならびにデータベースの構築 第１回 班会議, 18 Oct. 2023, Japanese
18 Oct. 2023 - 19 Oct. 2023

ダイナミックエピトープ -未開拓標的分子からの革新的創薬コンセプト-　　　　　　　　　　　　　　　
西村紳一郎
JST新技術説明会, 05 Oct. 2023, Japanese
[Invited]

The dynamic epitope theory : Structural and molecular insight into antibody recognition of pancreatic cancer MUC1　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
I GlycoForum La Rioja-Euskadi-Hokkaido, 07 Sep. 2023, English, Keynote oral presentation
07 Sep. 2023 - 08 Sep. 2023, [Invited]

The dynamic epitope theory　　　　　　　　　　　　　　　
Shi-Ichiro Nishimura
The 13th International Polymer Conference (IPC2023), 20 Jul. 2023, English, Keynote oral presentation

エクソソームのユニークな構造と機能に学ぶ新しい創薬研究への展開　　　　　　　　　　　　　　　
西村紳一郎
官民研究開発投資拡大プログラム（PRISM） 「新薬創出を加速する人工知能の開発」 令4元年度成果報告会, 13 Mar. 2023, Japanese

実践的糖鎖工学プラットフォームの構築と次世代DDSの創成　　　　　　　　　　　　　　　
西村紳一郎
第12回GFRGプロジェクトシンポジウム, 19 Jan. 2023, Japanese, Oral presentation

Creation of an innovative drug delivery system targeting pre-metastatic niche　　　　　　　　　　　　　　　
西村紳一郎
第9回日本細胞外小胞学会, 25 Oct. 2022, Japanese, Invited oral presentation
24 Oct. 2022 - 25 Oct. 2022, [Invited]

血中エクソソームの由来臓器を糖鎖解析により推定する基盤技術の開発　　　　　　　　　　　　　　　
西村紳一郎
官民研究開発投資拡大プログラム（PRISM） 「新薬創出を加速する人工知能の開発」 令和3年度成果報告会, 12 May 2022, Japanese

Antibodies recognizing dynamic neoepitopes generated by cancer-specific truncated immature O-glycosylation　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
The 2021 International Chemical Congress of Pacific Basin Societies / Pacifichem2021, 11 Dec. 2021, English, Invited oral presentation
16 Dec. 2021 - 21 Dec. 2021, [Invited]

The dynamic epitope theory-Vulnerability of proteins induced by posttranslational glycosylation　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
70th Symposium on Macromolecules, 06 Sep. 2021, Japanese, Invited oral presentation
06 Sep. 2021 - 08 Sep. 2021, [Invited]

動的エピトープ理論による革新的創薬　　　　　　　　　　　　　　　
西村紳一郎
LIFE SCIENCE CONEECT 2021, 26 Aug. 2021, Japanese

ヒト組織由来エクソソームの糖鎖プロファイリングによる新しいバイオマーカーの探索　　　　　　　　　　　　　　　
西村紳一郎
官民研究開発投資拡大プログラム（PRISM） 「新薬創出を加速する人工知能の開発」 令和二年度成果報告会, 20 Jul. 2021, Japanese

ローマは一日にして成らず。されど、すべての道は？　　　　　　　　　　　　　　　
西村紳一郎
GlycoTOKYO2019, 16 Nov. 2019, Japanese, Invited oral presentation
[Invited]

Smart nanomedicine targeting of lysosomal glycohydrolases through activated endocytosis of cancer cells　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
25th International Symposium on Glycoconjugates, Aug. 2019, English, Poster presentation
25 Aug. 2018 - 31 Aug. 2019

Experience feedback from Hokkaido University researchers cooperating with European institutions, FP7 (Seventh Framework Programme, 2007-2013), “GlycoHIT: Glycomics by High-throughput Integrated Technologies” and “GLYCOPHARM: The sugar code from biochemical concept to clinics”　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
EURAXESS Japan Tour 2019, 07 Mar. 2019, English, Nominated symposium
[Invited]

独創的なシステム糖鎖工学プラットフォームからの創薬イノベーション　　　　　　　　　　　　　　　
西村紳一郎
医薬基盤研究所創薬教育セミナー, 07 Mar. 2018, Japanese, Public discourse
[Invited]

糖鎖研究の現状と将来展望　　　　　　　　　　　　　　　
西村紳一郎
第9回GFRG研究会シンポジウム, 08 Dec. 2017, Japanese, Nominated symposium

動的エピトープ理論：疾患特異抗原成立の新原理に基づく合理的創薬システムの構築　　　　　　　　　　　　　　　
西村紳一郎
第9回Neuroprotective Meeting for Young Researchers, 04 Nov. 2017, Japanese, Public discourse
[Invited]

動的エピトープ理論：タンパク質のダイナミックな翻訳後修飾が創出する疾患特異的抗原構造　　　　　　　　　　　　　　　
西村 紳一郎
第57回日本臨床化学会年次学術集会, 06 Oct. 2017, Japanese, Invited oral presentation
[Domestic Conference]

〔Major achievements〕Nanomedicine targeting intracellular glycoenzymes　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
2017 Gordon Research Conference (GRC) on Carbohydrate, 29 Jun. 2017, English, Invited oral presentation
[Invited], [International presentation]

New glycotechnology platform interfacing chemistry and biology　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
253rd ACS National Meeting, 05 Apr. 2017, English, Invited oral presentation
[Invited], [International presentation]

A theory of the dynamic epitope: Autoantibodies to the dynamic epitopes generated by aberrant glycosylation at consecutive threonine motifs in circulating carcinoma mucins　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
日本化学会第97春季年会, 19 Mar. 2017, English, Oral presentation
[Domestic Conference]

Chemical Synthesis Uncovers Novel Regulation Mechanism in Protein Folding by Site-specific Glycosylation　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
A3 RONA and CPRH, 24 Sep. 2016, English, Oral presentation
[Invited], [International presentation]

〔Major achievements〕New trends in glycodrug discovery: Molecular targets unveiled by chemical synthesis and nanotechnology　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
GLYCOPHARM Final Conference, 27 Jul. 2016, English, Invited oral presentation
[Invited], [International presentation]

動的な翻訳後修飾による疾患特異的抗原構造の獲得　　　　　　　　　　　　　　　
西村 紳一郎
北大・部局横断シンポジウム：研究ネットワーク促進プログラム「生体防御システムとその破綻」～免疫・感染・癌・炎症～, 07 Mar. 2016, English, Invited oral presentation
[Invited], [Domestic Conference]

〔Major achievements〕Toward personalized medicine: Chemical and enzymatic synthesis of disease-relevant glycoproteins　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
103rd Indian Science Congress 2016, 05 Jan. 2016, English, Keynote oral presentation
[Invited], [International presentation]

Posttranslational glycosylation and cancer cell's life cycle: From discovery of disease-relevant epitope to development of new class anti-cancer drugs　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
第12回消化器外科Ⅰモーニングセミナー, 17 Nov. 2015, English, Public discourse
[Invited], [Domestic Conference]

EPITOPE-DEFINED ANTIBODIES AS NEW CLASS THERAPEUTIC AND DIAGNOSTIC REAGENTS: TOWARD PERSONALIZED MEDICINE FROM CHEMICAL GLYCOBIOLOGY　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
GLYCO23, 17 Sep. 2015, English, Keynote oral presentation
15 Sep. 2015 - 20 Sep. 2015, [Invited]

〔Major achievements〕EPITOPE-DEFINED ANTIBODIES AS NEW CLASS THERAPEUTIC AND DIAGNOSTIC REAGENTS: TOWARD PERSONALIZED MEDICINE FROM CHEMICAL GLYCOBIOLOGY　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
EMBO Workshop, 21 Jun. 2015, English, Keynote oral presentation
[Invited], [International presentation]

Epitope-defined antibody drugs discovered by novel glycotechnology　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
基礎血液談話会, 24 Apr. 2015, English, Invited oral presentation
[Invited], [Domestic Conference]

Intracellular traffic of cell surface mimetic quantum dots-anchored glycopeptides　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
249th ACS National Meeting & Exposition, 24 Mar. 2015, English, Invited oral presentation
[Invited], [International presentation]

〔Major achievements〕Epitope-defined antibody discovered by new glycotechnology　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
2015 Queenstown Molecular Biology Meeting, 19 Mar. 2015, English, Invited oral presentation
[Invited], [International presentation]

Epitope-defined antibody as new class therapeutic and diagnostic reagents: Toward personalized medicine from chemical glycobiology　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
International Symposium on Chemical Biology Approach to Metabiomics, Chemical genomics and Epigenomics, 19 Feb. 2015, English, Keynote oral presentation
[Invited], [International presentation]

糖鎖研究からの医薬品開発 - QuaDRAD™システムの構築と抗体医薬の創出　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
Clinical Oncology Research勉強会, 27 Nov. 2014, Japanese, Public discourse
[Invited], [Domestic Conference]

Toward personalized medicine from glycan biomarkers discovered by glycoblotting-assisted high throughput serum glycomics　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
Sino-Japan Workshop on Chemical Biology, 12 Oct. 2014, English, Invited oral presentation
[Invited], [International presentation]

〔Major achievements〕GLYCOPHARM at work with glycopeptide epitopes: promising targets uncovered by comprehensive glycomics and robust synthetic compound library　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
GLYCOPHARM meeting, 26 Jun. 2014, English, Invited oral presentation
[Invited], [International presentation]

〔Major achievements〕Antigenic glycopeptides: Conformational impact of protein glycosylation and disease-relevant antigenic structure　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
Special Lecture at Johns Hopkins School of Medicine, 25 Mar. 2014, English, Public discourse
[Invited], [International presentation]

High Performance Glycopeptide Microarray: Rapid Epitope Profiling of Monoclonal Antibodies and Serum Autoantibodies　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
27th International Carbohydrate Symposium (ICS2014), 13 Jan. 2014, English, Invited oral presentation
[Invited], [International presentation]

Toward personalized medicine from glycan biomarkers discovered by glycoblotting-assisted high throughput serum glycomics　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
International Symposium on Chemical Biology – Drug Discovery, 09 Jan. 2014, English, Invited oral presentation
[Invited], [International presentation]

Autoantibodies to cancer-associated MUC1 fragments in healthy human sera discovered by high performance glycopeptide microarray platform　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
246th ACS National Meeting & Expositions, 08 Sep. 2013, English, Invited oral presentation
[Invited], [International presentation]

Drug discovery from chemical glycobiology　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
Special seminar at Wayne State University, 06 Sep. 2013, English, Public discourse
[Invited], [International presentation]

Toward personalized medicine from chemical glycobiology　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
GlycoMedicine Seminar, 20 Aug. 2013, English, Invited oral presentation
[Invited], [International presentation]

糖鎖研究からの創薬－新しい抗体医薬品の開発を目指して　　　　　　　　　　　　　　　
西村 紳一郎
Conference for BioSignal and Medicine2013（CBSM2013）, 14 Jul. 2013, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Toward personalized medicine from glycan biomarkers discovered by glycoblotting-assisted high throughput serum glycomics　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
8th ISABS(International Society for Applied Biological Sciences) Conference, 28 Jun. 2013, English, Invited oral presentation
[Invited], [International presentation]

学問の風景－Be ambitious!を胸に　　　　　　　　　　　　　　　
西村 紳一郎
学校法人高宮学園 代々木ゼミナール新校舎開校記念特別講演, 03 Apr. 2013, Japanese, Public discourse
[Invited], [Domestic Conference]

人生に解なし―勇気を出してそれぞれの大志を抱け　　　　　　　　　　　　　　　
西村 紳一郎
北嶺中高等学校 進路講演会, 15 Dec. 2012, Japanese, Public discourse
[Invited], [Domestic Conference]

生活習慣病・病気と糖鎖の関係　　　　　　　　　　　　　　　
西村 紳一郎
農林水産省委託プロジェクト研究「農林水産物・食品の機能性等を解析・評価するための基盤技術の開発」 第2回 医と食の市民公開講座, 11 Nov. 2012, Japanese, Public discourse
[Invited], [Domestic Conference]

Regulation of glycan biosynthetic mechanism and drug discovery　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
第17回 未来創薬・医療イノベーションセミナー, 12 Sep. 2012, English, Invited oral presentation
[Invited], [International presentation]

Development and clinical application of fully automated glycan analytical system　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
JASIS2012, 06 Sep. 2012, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Glycomics and Drug Discovery　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
26th International Carbohydrate Symposium, 23 Jul. 2012, English, Invited oral presentation
[Invited], [International presentation]

Toward Fully Automated Glycomics by Glycoblotting Method　　　　　　　　　　　　　　　
Shin-Ichiro Nishimura
Increasing the Impact of Glycoscience through New Tools and Technologies （Satellite Meeting of ICS2012）、、主催：Niels-Christian Reichardt group leader at CIC biomaGUNE, 20 Jul. 2012, English, Keynote oral presentation
[Invited], [International presentation]

グライコブロッティング法と糖鎖ナノテクノロジー　　　　　　　　　　　　　　　
西村 紳一郎
第16回腸内細菌学会, 15 Jun. 2012, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

各種質量分析システムを用いた糖タンパク質の特性解析 ～バイオ医薬品の開発における合理的アプローチ～　　　　　　　　　　　　　　　
西村 紳一郎
AB SCIEX LC/MS ユーザーズミーティング2012, 21 Mar. 2012, Japanese, Public discourse
[Invited], [Domestic Conference]