Nov. 2022, 日本水処理生物学会, Paper award
Isolation and Characterization of Novel Plant Growth-Promoting Bacteria from the Fronds of Duckweed
Iwashita T;Tanaka Y;Tamaki H;Nakai R;Yoneda Y;Makino A;Toyama T;Kamagata Y;Morikawa M;Mori K, 29262199

Oct. 2022, Kasetsart University, Thailand, Honorary Doctorate of Philosophy (Bioscience)　　　　　　　　　　　　　　　

Nov. 2019, 日本水処理生物学会, Paper award
Biomass production and nutrient removal through cultivation of Euglena gracilis in domestic wastewater
Masashi Kuroda;Taro Horino;Daisuke Inoue;Masaaki Morikawa;Michihiko Ike, 10645598

2017, ISCDRA, First Place “2017 Knowing to Growing Duckweed Application Award”　　　　　　　　　　　　　　　
Evaluation of environmental bacterial communities as a factor affecting the growth of duckweed Lemna minor
H. Ishizawa;M. Kuroda;M. Morikawa;M. Ike

2011, 日本農芸化学会, 第8回農芸化学研究企画賞　　　　　　　　　　　　　　　
Japan

2007, 天野エンザイム, 第8回 酵素応用シンポジウム研究奨励賞　　　　　　　　　　　　　　　
Japan

2003, バイオインダストリー協会, 奨励賞　　　　　　　　　　　　　　　
発酵と代謝
Japan

1999, 日本生物工学会, 第7回生物工学論文賞　　　　　　　　　　　　　　　
森川 正章;岩佐 毅;柳田 祥三;今中 忠行;阪大院;工;京大院

1994, 日本生物工学会, 第2回生物工学論文賞　　　　　　　　　　　　　　　
森川 正章;今中 忠行

1993, 日本生物工学会, 第1回生物工学論文賞　　　　　　　　　　　　　　　
森川 正章;伊東 幹人;今中 忠行

Enhancement of the duckweed biomass and starch production utilizing biogenic MnO and ZnO microparticles.
Maryam Anar, Sajjad Kamal Shuvro, Muhammad Farooq Hussain Munis, Masaaki Morikawa
Biotechnology reports (Amsterdam, Netherlands), 47, e00907, Sep. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, The effects of biologically synthesized MnO and ZnO microparticles (MPs), were evaluated on the growth of Lemna minor. Both MnO and ZnO MPs promoted the growth of L. minor at low concentrations of 1-8 mg/L. In contrast, 1000 mg/L MnO and ZnO MPs reduced the growth of L. minor. It was also found that the starch content of L. minor and Spirodela polyrhiza was enhanced upon growing in 1 mg/L of MnO MPs when compared to no MnO MPs. These effects were suggested to be due in part to the fact that biogenic MPs harbored small but significant amounts of inorganic phosphorus. Our findings indicate that biologically synthesized MnO and ZnO MPs can act as novel plant growth regulators to enhance the production of useful duckweed biomass with high starch content., 29262199

Creation of a functional duckweed holobiont to reduce nutrient competition with microalgae for high-yield biomass production.
Huyền Thị Thanh Phạm, Shohei Kuroda, Yeni Khairina, Masaaki Morikawa
Bioresource technology, 421, 132110, 132110, 28 Jan. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Duckweed has been highlighted as an appropriate biomass for low-carbon industries because of its significantly high production rate and multiple resource value. However, the outbreak of microalgae is a practical issue that decreases duckweed production yield. This study demonstrated that the growth of the duckweed Lemna aequinoctialis from factory wastewater was enhanced by colonization with indigenous plant growth-promoting bacteria (PGPB), whereas the growth of a duckweed competitor microalga, Coelastrella sp. KC10, from the same wastewater was reduced by indigenous microalgal growth-inhibiting bacteria (MGIB). Finally, a quadruple co-culture of a synthetic duckweed holobiont, L. aequinoctialis colonized by both KLaR20 (PGPB) and KLaR16 (MGIB), and Coelastrella sp. KC10 successfully recovered the duckweed production level by 117.5% in frond number and 84.5% in dry weight compared to those in the absence of microalgae. This case study demonstrates for the first time that duckweed holobionts can be reconstructed and enforced to antagonize growth competitor microalgae., 29262199

Evaluation of sewage treatment performance and duckweed biomass production efficiency in a primary sedimentation basin + duckweed pond + downflow hanging sponge (PSB + DWP + DHS) system
Kengo Kubota, Takashi Otani, Teruki Hariu, Takumi Jin, Tadashi Tagawa, Masaaki Morikawa, Yu-You Li
Journal of Water Process Engineering, Aug. 2024, [Peer-reviewed]
English, Scientific journal, 29262199

Sulfamethoxazole removal and fuel-feedstock biomass production from wastewater in a phyto-Fenton process using duckweed culture.
Tadashi Toyama, Maki Kobayashi, Rubiy Atno, Masaaki Morikawa, Kazuhiro Mori
Chemosphere, 361, 142592, 142592, Aug. 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, The phyto-Fenton process, which generates hydroxyl radicals through Fenton and Fenton-like reactions using plant-derived hydrogen peroxide (H2O2) and ferrous iron (Fe (II)) can degrade organic pollutants. Duckweed, an aquatic plant, is promising for a co-beneficial phytoremediation process that combines wastewater treatment and biomass production for biofuel feedstock. However, the phyto-Fenton process using duckweed has not been extensively studied. Because sulfamethoxazole (SMX), a major antibiotic, is distributed widely and is an emerging contaminant, its effective removal from contaminated water is necessary. The present study investigated the possibility of the simultaneous efficient removal of SMX from polluted water and biomass production for fuel feedstock by the phyto-Fenton process using duckweed. This is the first attempt to demonstrate the co-benefits of SMX removal and biomass production using duckweed. Intracellular H2O2 was produced using four duckweeds, Lemna aequinoctialis, L. minor, Landolina punctata, and Spirodela polyrhiza, in the range of 16.7-24.6 μ mol g-1 fresh weight, and extracellular H2O2 was released into the water phase. Consequently, duckweed could be used as an H2O2 supply source for the phyto-Fenton process. Specifically, 0.5 g fresh duckweed almost completely eliminated 1 mg L-1 SMX after 5 d in 50 mL sterile modified Hoagland solution containing 10 mM Fe (II). Fe (II)-dependent elimination of SMX indicated the occurrence of phyto-Fenton reaction. The phyto-Fenton process using duckweed effectively removed SMX. S. polyrhiza duckweed similarly removed 1 mg L-1 SMX even in sewage effluent containing other organic contaminants. During this treatment, duckweed biomass was generated at 7.95 g dry weight m-2 d-1, which was converted into methane at 353 normal liters CH4 kg-1 volatile solids by anaerobic digestion. For the first time, this study clearly demonstrates the potential for simultaneous SMX removal and biomass production from SMX-contaminated wastewater using duckweed., 29262199

A novel alkane monooxygenase evolved from a broken piece of ribonucleotide reductase in Geobacillus kaustophilus HTA426 isolated from Mariana Trench.
Tanasap Nithimethachoke, Chanita Boonmak, Masaaki Morikawa
Extremophiles : life under extreme conditions, 28, 1, 18, 18, 14 Feb. 2024, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, We have accidentally found that a thermophilic Geobacillus kaustophilus HTA426 is capable of degrading alkanes although it has no alkane oxygenating enzyme genes. Our experimental results revealed that a putative ribonucleotide reductase small subunit GkR2loxI (GK2771) gene encodes a novel heterodinuclear Mn-Fe alkane monooxygenase/hydroxylase. GkR2loxI protein can perform two-electron oxidations similar to homonuclear diiron bacterial multicomponent soluble methane monooxygenases. This finding not only answers a long-standing question about the substrate of the R2lox protein clade, but also expands our understanding of the vast diversity and new evolutionary lineage of the bacterial alkane monooxygenase/hydroxylase family.

Successful Isolation of Diverse Verrucomicrobiota Strains through the Duckweed-Microbes Co-cultivation Method.
Yasuhiro Tanaka, Erina Tozawa, Tomoki Iwashita, Yosuke Morishita, Hideyuki Tamaki, Tadashi Toyama, Masaaki Morikawa, Yoichi Kamagata, Kazuhiro Mori
Microbes and environments, 39, 3, 2024, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The "duckweed-microbes co-cultivation method" is a microbial isolation technique that effectively recovers diverse microbes, including rarely cultivated bacterial phyla, from environmental samples. In this method, aseptic duckweed and microbes collected from an environmental sample are co-cultivated for several days, and duckweed-associated microbes are then isolated from its roots using a conventional agar plate-based cultivation method. We herein propose several improvements to the method in order to specifically obtain members of the rarely cultivated bacterial phylum, Verrucomicrobiota. In systems using river water as the inoculum, the marked enrichment of Verrucomicrobiota was observed after 10 days of co-cultivation, particularly in the roots and co-cultivated media. We also successfully isolated 44 strains belonging to subdivisions 1, 3, and 4 of the phylum Verrucomicrobiota from these systems. This was achieved by changing the concentration of nitrogen in the co-cultivation medium, which is known to affect duckweed growth and/or metabolism, and by subjecting the fronds and co-cultivated media as well as the roots after co-cultivation to microbial isolation.

Duckweed-associated bacteria as plant growth-promotor to enhance growth of Spirodela polyrhiza in wastewater effluent from a poultry farm.
Chanita Boonmak, Sirapat Kettongruang, Buranaporn Buranathong, Masaaki Morikawa, Kannika Duangmal
Archives of microbiology, 206, 1, 43, 43, 26 Dec. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Duckweed has been highlighted as an invaluable resource because of its abilities to remove nitrogen and phosphorus from wastewater coupling with the production of high starch/protein-containing plant biomass. Duckweed recruits microbes and particularly forms a stable "core" bacterial microbiota, which greatly reduces the colonization efficiency of plant growth-promoting bacteria (PGPB). In this study, natural duckweeds were enriched in a sterilized-partially treated wastewater effluent from a poultry farm. After 24 days of cultivation, the duckweed-associated bacteria (DAB) were isolated and evaluated for their plant growth-promoting (PGP) potentials by co-cultivation with axenic Spirodela polyrhiza. Ten species were found in more than one location and could be considered candidates for the stable "core" DAB. Among them, all isolates of Acinetobacter soli, Acidovorax kalamii, Brevundimonas vesicularis, Pseudomonas toyotomiensis, and Shinella curvata increased duckweed growth in Hoagland medium. The highest PGP ability was observed in Sh. curvata W12-8 (with EPG value of 208.72%), followed by Paracoccus marcusii W7-16 (171.31%), Novosphingobium subterraneum W5-13 (156.96%), and Ac. kalamii W7-18 (156.96%). However, the highest growth promotion in the wastewater was observed when co-cultured with W7-16, which was able to increase biomass dry weight and root length of duckweed by 3.17 and 2.26 folds, respectively., 29262199

Gene cloning and characterization of a vanadium-dependent bromoperoxidase from the red alga Laurencia saitoi, a producer of brominated diterpenoids and triterpenoids
Kensuke Kaneko, Daiki Kobayashi, Shiro Masaki, Kenji Washio, Masaaki Morikawa, Tatsufumi Okino
JOURNAL OF APPLIED PHYCOLOGY, Apr. 2023, [Peer-reviewed]
English, Scientific journal

Spontaneous Cell Lysis by <i>Pelomonas saccharophila</i> MRB3 Provides Plant-Available Macronutrients in Hydroponic Growth Media and Accelerates Biomass Production of Duckweed
Hidehiro Ishizawa, Yukiko Kaji, Yuki Shimizu, Masashi Kuroda, Daisuke Inoue, Ayaka Makino, Ryosuke Nakai, Hideyuki Tamaki, Masaaki Morikawa, Michihiko Ike
Journal of Water and Environment Technology, 21, 1, 49, 58, Japan Society on Water Environment, Jan. 2023, [Peer-reviewed]
Scientific journal

Characterization of vanadium-dependent bromoperoxidases involved in the production of brominated sesquiterpenes by the red alga Laurencia okamurae
Takafumi Ishikawa, Kenji Washio, Kensuke Kaneko, Xiao Rong Tang, Masaaki Morikawa, Tatsufumi Okino
Applied Phycology, 3, 1, 120, 131, Informa UK Limited, 31 Dec. 2022, [Peer-reviewed]
Scientific journal

Diazotrophic bacterium Azotobacter vinelandii as a mutualistic growth promoter of an aquatic plant: Lemna minor
Sajjad Kamal Shuvro, Rahul Jog, Masaaki Morikawa
Plant Growth Regulation, Springer Science and Business Media LLC, 21 Sep. 2022, [Peer-reviewed], [Corresponding author]
Scientific journal, 29262199

Nostosin G and Spiroidesin B from the Cyanobacterium Dolichospermum sp. NIES-1697
Chin-Soon Phan, Jakia Jerin Mehjabin, Andrea Roxanne J. Anas, Masahiro Hayasaka, Reiko Onoki, Juting Wang, Taiki Umezawa, Kenji Washio, Masaaki Morikawa, Tatsufumi Okino
Journal of Natural Products, American Chemical Society (ACS), 10 Aug. 2022, [Peer-reviewed]
Scientific journal

Isolation of Aquatic Plant Growth-Promoting Bacteria for the Floating Plant Duckweed (Lemna minor)
Ayaka Makino, Ryosuke Nakai, Yasuko Yoneda, Tadashi Toyama, Yasuhiro Tanaka, Xian-Ying Meng, Kazuhiro Mori, Michihiko Ike, Masaaki Morikawa, Yoichi Kamagata, Hideyuki Tamaki
Microorganisms, 10, 8, 1564, 1564, MDPI AG, 03 Aug. 2022, [Peer-reviewed]
Scientific journal, Plant growth-promoting bacteria (PGPB) can exert beneficial growth effects on their host plants. Little is known about the phylogeny and growth-promoting mechanisms of PGPB associated with aquatic plants, although those of terrestrial PGPB have been well-studied. Here, we report four novel aquatic PGPB strains, MRB1–4 (NITE P-01645–P-01648), for duckweed Lemna minor from our rhizobacterial collection isolated from Lythrum anceps. The number of L. minor fronds during 14 days co-culture with the strains MRB1–4 increased by 2.1–3.8-fold, compared with an uninoculated control; the plant biomass and chlorophyll content in co-cultures also increased. Moreover, all strains possessed an indole-3-acetic acid production trait in common with a plant growth-promoting trait of terrestrial PGPB. Phylogenetic analysis showed that three strains, MRB-1, -3, and -4, were affiliated with known proteobacterial genera (Bradyrhizobium and Pelomonas); this report is the first to describe a plant-growth promoting activity of Pelomonas members. The gammaproteobacterial strain MRB2 was suggested to be phylogenetically novel at the genus level. Under microscopic observation, the Pelomonas strain MRB3 was epiphytic and adhered to both the root surfaces and fronds of duckweed. The duckweed PGPB obtained here could serve as a new model for understanding unforeseen mechanisms behind aquatic plant-microbe interactions., 10645598

Complete Genome Sequence of Luteitalea sp. Strain TBR-22.
Kyosuke Yamamoto, Yasuko Yoneda, Ayaka Makino, Yasuhiro Tanaka, Xian-Ying Meng, Junko Hashimoto, Kazuo Shin-Ya, Noriyuki Satoh, Manabu Fujie, Tadashi Toyama, Kazuhiro Mori, Michihiko Ike, Masaaki Morikawa, Yoichi Kamagata, Hideyuki Tamaki
Microbiology resource announcements, 11, 2, e0045521, 17 Feb. 2022, [International Magazine]
English, Scientific journal, We report a complete genome sequence of a novel bacterial isolate, strain TBR-22, belonging to the class Vicinamibacteria of the phylum Acidobacteria, which was isolated from duckweed fronds. The genome expands our knowledge of the lifestyle of this abundant but rarely characterized phylum.

Draft Genome Sequence of Bryobacteraceae Strain F-183.
Kyosuke Yamamoto, Yasuko Yoneda, Ayaka Makino, Yasuhiro Tanaka, Xian-Ying Meng, Junko Hashimoto, Kazuo Shin-Ya, Noriyuki Satoh, Manabu Fujie, Tadashi Toyama, Kazuhiro Mori, Michihiko Ike, Masaaki Morikawa, Yoichi Kamagata, Hideyuki Tamaki
Microbiology resource announcements, 11, 1, e0045321, 20 Jan. 2022, [International Magazine]
English, Scientific journal, Here, we report a draft genome sequence of a bacterial strain, F-183, isolated from a duckweed frond. Strain F-183 belongs to the family Bryobacteraceae of the phylum Acidobacteria, and its genomic information would contribute to understanding the ecophysiology of this abundant but rarely characterized phylum.

Growth Promotion of Giant Duckweed Spirodela polyrhiza (Lemnaceae) by Ensifer sp. SP4 Through Enhancement of Nitrogen Metabolism and Photosynthesis
Tadashi Toyama, Kazuhiro Mori, Yasuhiro Tanaka, Michihiko Ike, Masaaki Morikawa
MOLECULAR PLANT-MICROBE INTERACTIONS, 35, 1, 28, 38, Jan. 2022, [Peer-reviewed]
English, Scientific journal, 29262199

Construction of a black-dirt formation model using microorganisms obtained from the toilet bowl　　　　　　　　　　　　　　　
Michitaka Muramatsu, Chie Ito, Kazuma Niizeki, Mitsuhiro Gomi, Masaaki Morikawa
Journal of Environmental Biotechnology, 21, 1, 63, 66, Jul. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Novel Plant-Associated Acidobacteria Promotes Growth of Common Floating Aquatic Plants, Duckweeds
Yasuko Yoneda, Kyosuke Yamamoto, Ayaka Makino, Yasuhiro Tanaka, Xian-Ying Meng, Junko Hashimoto, Kazuo Shin-ya, Noriyuki Satoh, Manabu Fujie, Tadashi Toyama, Kazuhiro Mori, Michihiko Ike, Masaaki Morikawa, Yoichi Kamagata, Hideyuki Tamaki
MICROORGANISMS, 9, 6, Jun. 2021, [Peer-reviewed]
English, Scientific journal

Indigenous bacteria, an excellent reservoir of functional plant growth promoters for enhancing duckweed biomass yield on site
Yeni Khairina, Rahul Jog, Chanita Boonmak, Tadashi Toyama, Tokitaka Oyama, Masaaki Morikawa
Chemosphere, 268, 129247, 129247, Elsevier {BV}, Apr. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal, 10645598

A cyclic lipopeptide surfactin is a species-selective Hsp90 inhibitor that suppresses cyanobacterial growth
Hitoshi Nakamoto, Yuhei Yokoyama, Takahiro Suzuki, Yuri Miyamoto, Takashi Fujishiro, Masaaki Morikawa, Yoshihiko Miyata
The Journal of Biochemistry, 170, 2, 255, 264, Oxford University Press (OUP), 26 Mar. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal,
Heat shock protein 90 (Hsp90) is essential for eukaryotic cells, whereas bacterial homologs play a role under stresses and in pathogenesis. Identifying species-specific Hsp90 inhibitors is challenging because Hsp90 is evolutionarily conserved. We found that a cyclic lipopeptide surfactin inhibits the ATPase activity of Hsp90 from the cyanobacterium Synechococcus elongatus (S.elongatus) PCC 7942 but does not inhibit Escherichia coli (E.coli), yeast and human Hsp90s. Molecular docking simulations indicated that surfactin could bind to the N-terminal dimerization interface of the cyanobacterial Hsp90 in the ATP- and ADP-bound states, which provided molecular insights into the species-selective inhibition. The data suggest that surfactin inhibits a rate-limiting conformational change of S.elongatus Hsp90 in the ATP hydrolysis. Surfactin also inhibited the interaction of the cyanobacterial Hsp90 with a model substrate, and suppressed S.elongatus growth under heat stress, but not that of E.coli. Surfactin did not show significant cellular toxicity towards mammalian cells. These results indicate that surfactin inhibits the cellular function of Hsp90 specifically in the cyanobacterium. The present study shows that a cyclic peptide has a great specificity to interact with a specific homolog of a highly conserved protein family.

Multiple biosurfactant production by Aureobasidium pullulans strain YTP6-14 in aqueous and heavy oil layers.
Natwara Amatyakul, Suthep Thaniyavarn, Masaaki Morikawa, Jiraporn Thaniyavarn
The Journal of general and applied microbiology, 66, 6, 330, 338, 26 Feb. 2021, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Aureobasidium pullulans YTP6-14 was demonstrated to be an excellent multiple biosurfactant producer utilizing cheap carbon sources available in Thailand, including glycerol and cassava flour hydrolysate. A. pullulans YTP6-14 maximally produced 1.81 g/l biosurfactant in an aqueous layer (BS-AQ) in a medium containing glycerol, and 7.37 or 6.37 g/l biosurfactant in a heavy oil layer (BS-HO) in cassava flour hydrolysate or a glucose containing medium, respectively. Each BS-AQ and BS-HO had critical micelle concentration values of 41.32 mg/l and 13.51 mg/l, and both biosurfactants formed a stable food oil emulsion and reduced the amount of biofilms formed by Streptococcus sobrinus and Streptococcus mutans. BS-AQ and BS-HO were mainly composed of liamocins or exophilins and massoia lactone, respectively.

Isolation and Characterization of Novel Plant Growth-Promoting Bacteria from the Fronds of Duckweed
TOMOKI IWASHITA, YASUHIRO TANAKA, HIDEYUKI TAMAKI, RYOSUKE NAKAI, YASUKO YONEDA, AYAKA MAKINO, TADASHI TOYAMA, YOICHI KAMAGATA, MASAAKI MORIKAWA, KAZUHIRO MORI
Japanese Journal of Water Treatment Biology, 57, 1, 1, 9, Japanese Society of Water Treatment Biology, 2021, [Peer-reviewed]
Scientific journal

Community dynamics of duckweed-associated bacteria upon inoculation of plant growth-promoting bacteria
Hidehiro Ishizawa, Masashi Kuroda, Daisuke Inoue, Masaaki Morikawa, Michihiko Ike
FEMS Microbiology Ecology, 96, 7, Oxford University Press (OUP), 01 Jul. 2020, [Peer-reviewed]
English, Scientific journal, 10645598

Biosurfactants from Marine Cyanobacteria Collected in Sabah, Malaysia
Jakia Jerin Mehjabin, Liang Wei, Julie G. Petitbois, Taiki Umezawa, Fuyuhiko Matsuda, Charles S. Vairappan, Masaaki Morikawa, Tatsufumi Okino
Journal of Natural Products, 83, 6, 1925, 1930, American Chemical Society (ACS), 26 Jun. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Chemical investigation of the organic extract from Moorea bouillonii, collected in Sabah, Malaysia, led to the isolation of three new chlorinated fatty acid amides, columbamides F (1), G (2), and H (3). The planar structures of 1-3 were established by a combination of mass spectrometric and NMR spectroscopic analyses. The absolute configuration of 1 was determined by Marfey's analysis of its hydrolysate and chiral-phase HPLC analysis after conversion and esterification with Ohrui's acid, (1S,2S)-2-(anthracene-2,3-dicarboximido)cyclohexanecarboxylic acid. Compound 1 showed biosurfactant activity by an oil displacement assay. Related known fatty acid amides columbamide D and serinolamide C exhibited biosurfactant activity with critical micelle concentrations of about 0.34 and 0.78 mM, respectively.

Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl2 stresses
Le Thai Hang, Kazuhiro Mori, Yasuhiro Tanaka, Masaaki Morikawa, Tadashi Toyama
Bioprocess and Biosystems Engineering, 43, 6, 971, 980, Springer Science and Business Media LLC, Jun. 2020, [Peer-reviewed]
English, Scientific journal, 10645598

Antimicrobial electrospun fiber mat from gelatin and crude extract lipopeptides　　　　　　　　　　　　　　　
Tipnipa Yoochang, Jirarat Anuntagool, Suthep Thaniyavarn, Masaaki Morikawa, Jiraporn Thaniyavarn
Songklanakarin Journal of Science and Technology, 42, 1, 101, 108, Jan. 2020, [Peer-reviewed]
English, Scientific journal

Enhanced biomass production and nutrient removal capacity of duckweed via two-step cultivation process with a plant growth-promoting bacterium, Acinetobacter calcoaceticus P23
Hidehiro Ishizawa, Yuka Ogata, Yoshiyuki Hachiya, Ko-ichiro Tokura, Masashi Kuroda, Daisuke Inoue, Tadashi Toyama, Yasuhiro Tanaka, Kazuhiro Mori, Masaaki Morikawa, Michihiko Ike
Chemosphere, 238, 124682, 124682, Elsevier BV, Jan. 2020, [Peer-reviewed]
English, Scientific journal, 10645598

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3
Tadashi Toyama, Tsubasa Hanaoka, Koji Yamada, Kengo Suzuki, Yasuhiro Tanaka, Masaaki Morikawa, Kazuhiro Mori
Biotechnology for Biofuels, 12, 1, 205, 205, Dec. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Colonization and Competition Dynamics of Plant Growth-Promoting/Inhibiting Bacteria in the Phytosphere of the Duckweed Lemna minor
Hidehiro Ishizawa, Masashi Kuroda, Kanako Inoue, Daisuke Inoue, Masaaki Morikawa, Michihiko Ike
Microbial Ecology, 77, 2, 440, 450, 15 Feb. 2019, [Peer-reviewed]
English, Scientific journal, 10645598

Biomass production and nutrient removal through cultivation of Euglena gracilis in domestic wastewater
Masashi Kuroda, Taro Horino, Daisuke Inoue, Masaaki Morikawa, Michihiko Ike
Japanese Journal of Water Treatment Biology, 54, 4, 105, 113, Dec. 2018, [Peer-reviewed]
English, Scientific journal, 10645598

Effects of co-inoculation of two different plant growth-promoting bacteria on duckweed
Yusuke Yamakawa, Rahul Jog, Masaaki Morikawa
Plant Growth Regulation, 86, 2, 287, 296, Springer Science and Business Media {LLC}, 06 Nov. 2018, [Peer-reviewed], [Corresponding author]
English, Scientific journal, 10645598

Effect of Exogenous General Plant Growth Regulators on the Growth of the Duckweed Lemna minor
Utami, Desi, Kawahata, Ami, Sugawara, Masayuki, Jog, Rahul N., Miwa, Kyoko, Morikawa, Masaaki
Frontiers in Chemistry, 6, 251, Jul. 2018, [Peer-reviewed], [Corresponding author]
English, Scientific journal, 10645598

Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater effluent
Toyama, Tadashi, Kasuya, Mari, Hanaoka, Tsubasa, Kobayashi, Naoto, Tanaka, Yasuhiro, Inoue, Daisuke, Sei, Kazunari, Morikawa, Masaaki, Mori, Kazuhiro
Biotechnology For Biofuels, 11, 176, Jun. 2018, [Peer-reviewed]
Scientific journal

Comprehensive evaluation of nitrogen removal rate and biomass, ethanol, and methane production yields by combination of four major duckweeds and three types of wastewater effluent
Tadashi Toyama, Tsubasa Hanaoka, Yasuhiro Tanaka, Masaaki Morikawa, Kazuhiro Mori
Bioresource Technology, 250, 464, 473, Elsevier Ltd, 01 Feb. 2018, [Peer-reviewed]
English, Scientific journal

Production of massoia lactone by Aureobasidium pullulans YTP6-14 isolated from the Gulf of Thailand and its fragrant biosurfactant properties
S. Luepongpattana, J. Thaniyavarn, M. Morikawa
JOURNAL OF APPLIED MICROBIOLOGY, 123, 6, 1488, 1497, Dec. 2017, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Differential oxidative and antioxidative response of duckweed Lemna minor toward plant growth promoting/inhibiting bacteria
Hidehiro Ishizawa, Masashi Kuroda, Masaaki Morikawa, Michihiko Ike
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 118, 667, 673, Sep. 2017, [Peer-reviewed]
English, Scientific journal, 10645598

Enhanced biomass production of duckweeds by inoculating a plant growth-promoting bacterium, Acinetobacter calcoaceticus P23, in sterile medium and non-sterile environmental waters
T. Toyama, M. Kuroda, Y. Ogata, Y. Hachiya, A. Quach, K. Tokura, Y. Tanaka, K. Mori, M. Morikawa, M. Ike
WATER SCIENCE AND TECHNOLOGY, 76, 6, 1418, 1428, Sep. 2017, [Peer-reviewed]
English, Scientific journal

Evaluation of environmental bacterial communities as a factor affecting the growth of duckweed Lemna minor
Hidehiro Ishizawa, Masashi Kuroda, Masaaki Morikawa, Michihiko Ike
BIOTECHNOLOGY FOR BIOFUELS, 10, 62, Mar. 2017, [Peer-reviewed]
English, Scientific journal

Production of biosurfactant by Wickerhamomyces anomalus PY189 and its application in lemongrass oil encapsulation
Chanapa Dejwatthanakomol, Jirarat Anuntagool, Masaaki Morikawa, Jiraporn Thaniyavarn
SCIENCEASIA, 42, 4, 252, 258, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Wewakazole B, a Cytotoxic Cyanobactin from the Cyanobacterium Moorea producens Collected in the Red Sea
Julius Adam V. Lopez, Sultan S. Al-Lihaibi, Walied M. Alarif, Ahmed Abdel-Lateff, Yasuyuki Nogata, Kenji Washio, Masaaki Morikawa, Tatsufumi Okino
JOURNAL OF NATURAL PRODUCTS, 79, 4, 1213, 1218, Apr. 2016, [Peer-reviewed]
English, Scientific journal

Isolation and characterization of an early colonizing Rhizobium sp. R8 from a household toilet bowl
Toru Fukano, Mitsuhiro Gomi, Yukihiko Osaki, Masaaki Morikawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79, 7, 1207, 1215, Jul. 2015, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Wewakazole B, A New Cyclic Dodecapeptide from the Red Sea Cyanobacterium Moorea producens
Lopez Julius Adam Velasco, Al-Lihaibi Sultan S., Alarif Walied M., Abdel-Lateff Ahmed, Washio Kenji, Morikawa Masaaki, Okino Tatsufumi
Symposium on the Chemistry of Natural Products, symposium papers, 57, PosterP23, Symposium on the Chemistry of Natural Products Steering Committee, 2015
Japanese, Cyanobacteria are prolific sources of novel bioactive compounds. The chemical profiles we obtained using LC-MS and checked with the MarinLit database showed that there are potential new compounds from Moorea producens samples obtained from the Red Sea. In this presentation the isolation and structure elucidation of a new cyclic dodecapeptide, wewakazole B (1), is reported.

The brownish-red filamentous sample was obtained by SCUBA near Jeddah, Saudi Arabia. A small portion of the sample was preserved in RNAlater and identified as M. producens by standard 16S rRNA gene sequencing using cyanobacteria-specific primers 106F and 1509R, and internal primers 359F and 781R (Martinez-Murcia et al., 1995; Nubel et al., 1997). The bulk of the sample was extracted with methanol followed by solvent partition using EtOAc and water, then, BuOH. These extracts were tested for antifouling activity against the barnacle larvae Amphibalanus amphitrite, cytotoxicity against MCF-7 breast cancer cells, and trypsin inhibition. LC-MS was carried out in parallel for dereplication in conjunction with the MarinLit database. The extracts were found to be inactive but a unique [M+H]⁺ m/z of 1127 was detected in the EtOAc extract and was pursued. A series of open column chromatography and HPLCs afforded 0.4 mg of 1.

The molecular formula, C₅₈H₇₀N₁₂O₁₂, determined by HRESIMS ([M+H]⁺ m/z1127.5310, ∆ = 0.39 ppm), suggested thirty unsaturations. Dereplication using mass and ¹H NMR data showed wewakazole (Nogle et al., 2003) as the nearest candidate. HSQC, COSY, and TOCSY revealed nine common and three modified amino acid residues which were connected by HMBC and ROESY correlations acquiring five fragments. Connections to the oxazole and methyloxazole fragments proved to be a challenge due to the lack of correlations towards neighboring amino acids. All possible combinations were considered that lead to the most logical planar structure and cyclic sequence. Chiral LC-MS and HPLC analysis showed that all amino acid residues possess the L- configuration. The wewakazoles are the only cyclic peptides from marine cyanobacteria having both Oxz and MeOxz. Their function and bioactivity require further investigation.

Draft genome sequence of acinetobacter calcoaceticus strain P23, a plant growth-promoting bacterium of duckweed
Masayuki Sugawara, Akira Hosoyama, Atsushi Yamazoe, Masaaki Morikawa
Genome Announcements, 3, 1, e00026-15, American Society for Microbiology, 2015, [Peer-reviewed], [Corresponding author]
English, Scientific journal, 10645598

cDNA cloning and characterization of vanadium-dependent bromoperoxidases from the red alga Laurencia nipponica
Kensuke Kaneko, Kenji Washio, Taiki Umezawa, Fuyuhiko Matsuda, Masaaki Morikawa, Tatsufumi Okino
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 78, 8, 1310, 1319, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Plant growth-promoting bacterium Acinetobacter calcoaceticus P23 increases the chlorophyll content of the monocot Lemna minor (duckweed) and the dicot Lactuca sativa (lettuce)
Wakako Suzuki, Masayuki Sugawara, Kyoko Miwa, Masaaki Morikawa
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 118, 1, 41, 44, Jul. 2014, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Transformation of iso-pentylbenzene by a biofilm-forming strain of Candida viswanathii TH1 isolated from oil-polluted sediments collected in coastal zones in Vietnam
Le Thi Nhi Cong, Cung Thi Ngoc Mai, Masaaki Morikawa, Nghiem Ngoc Minh
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, 49, 7, 777, 786, Jun. 2014, [Peer-reviewed]
English, Scientific journal

Cloning and expression of three ladA-type alkane monooxygenase genes from an extremely thermophilic alkane-degrading bacterium Geobacillus thermoleovorans B23
Chanita Boonmak, Yasunori Takahashi, Masaaki Morikawa
EXTREMOPHILES, 18, 3, 515, 523, May 2014, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Bromoperoxidases from Laurencia species
Kaneko Kensuke, Washio Kenji, Umezawa Taiki, Kobayashi Daiki, Tang Xiaorong, Matsuda Fuyuhiko, Morikawa Masaaki, Okino Tatsufumi
Symposium on the Chemistry of Natural Products, symposium papers, 56, Poster53, Symposium on the Chemistry of Natural Products Steering Committee, 2014
Japanese, The genus of red alga Laurencia is one of the richest producers of halogenated compounds (mostly brominated) ³⁾. Murai et al ⁴⁾ and Butler et al ⁹⁾ showed enzymatic bromination of Laurencia C₁₅acetogenins including laurencin (1) ^1,2)and sesquiterpenoids snyderols using partially purified bromoperoxidase (BPO) from L. nipponica and vanadium-dependent BPO (VBPO) from a mixture of red algae including L. pacifica. However, since the lack of information of sequences, the structural information and biochemical aspects of BPO from L. nipponica and VBPO from L. pacifica remains unknown. Here, we report cDNA cloning of VBPOs from L. nipponicaand in vitro enzymatic bromination of putative biosynthetic precursors using recombinant enzymes.

Two VBPOs (LnVBPO1 and LnVBPO2) were cloned from L. nipponica and recombinant LnVBPOs were prepared. Recombinant LnVBPOs were subjected to enzyme activity assay. A general substrate, monochlorodimedone (MCD), was utilized for K_m determination for H₂O₂ and , essential for bromination of organic substrate. K_m for H₂O₂was 0.03 mM in both LnVBPOs, K_m for were 0.53 mM for LnVBPO1 and 0.35 mM for LnVBPO2, respectively. A derivative of putative precursor of laurencin (1), TMS-capped (3E, 6R, 7R)-laurediol (4) was subjected to LnVBPOs enzymatic bromination and gave similar MS/MS spectrum of deacetylaurencin (3). Since the compound 3 is considered to be the final precursor of 1, it is suggested that VBPO catalyzes bromination in Laurenciametabolites.

We also cloned VBPOs from other Laurencia spp. which produce brominated sesquiterpenoids (L. okamurae) and triterpenoids (L. saitoi). We will discuss in vitro enzymatic bromination of these two LaurenciaVBPOs.

Draft genome sequence of the biofilm-producing Bacillus subtilis strain B-1, isolated from an oil field
S. Kesel, F. Moormann, I. G�mperlein, A. Mader, M. Morikawa, O. Lieleg, M. Opitz
Genome Announcements, 2, 6, e01163-14, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Production and Characterization of a Biosurfactant from Cyberlindnera samutprakarnensis JP52(T)
Jamroonsri Poomtien, Jiraporn Thaniyavarn, Pairoh Pinphanichakarn, Sasitorn Jindamorakot, Masaaki Morikawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77, 12, 2362, 2370, Dec. 2013, [Peer-reviewed]
English, Scientific journal

Isolation and Characterization of a Thermotolerant Ammonia-Oxidizing Bacterium Nitrosomonas sp JPCCT2 from a Thermal Power Station
Yoshikane Itoh, Keiko Sakagami, Yoshihito Uchino, Chanita Boonmak, Tetsuro Oriyama, Fuyumi Tojo, Mitsufumi Matsumoto, Masaaki Morikawa
MICROBES AND ENVIRONMENTS, 28, 4, 432, 435, Dec. 2013, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Draft genome sequence of Geobacillus thermoleovorans strain B23
Chanita Boonmak, Yasunori Takahasi, Masaaki Morikawa
Genome Announcements, 1, 6, e00944-13, American Society for Microbiology, 2013, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Sustainable biodegradation of phenolic endocrine-disrupting chemicals by Phragmites australis-rhizosphere bacteria association
T. Toyama, T. Ojima, Y. Tanaka, K. Mori, M. Morikawa
WATER SCIENCE AND TECHNOLOGY, 68, 3, 522, 529, 2013, [Peer-reviewed]
English, Scientific journal

バイオフィルムをしらべてみよう　　　　　　　　　　　　　　　
森川 正章
生物工学会誌, 90, 246, 250, May 2012, [Invited], [Lead author, Corresponding author]
Japanese

Efficacy of forming biofilms by naphthalene degrading Pseudomonas stutzeri T102 toward bioremediation technology and its molecular mechanisms
Kohei Shimada, Yoshikane Itoh, Kenji Washio, Masaaki Morikawa
CHEMOSPHERE, 87, 3, 226, 233, Apr. 2012, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Unique Microbiological Characteristics of Pseudoalteromonas spp. that Form Biofilms in the Sea Water Environment : Their Possible Contribution to Water Purification and Fish Farming
IIJIMA Saori, OKAHARA Ryota, WASHIO Kenji, MORIKAWA Masaaki
Technical Report on Salt Science, 66, 4, 186, 190, The Society of Sea Water Science, Japan, 2012
Japanese

A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3 ',5 '-Bispyrophosphate Level in Pseudomonas sp MIS38
Kenji Washio, Siew Ping Lim, Niran Roongsawang, Masaaki Morikawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75, 10, 1880, 1888, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Diversity of Nonribosomal Peptide Synthetases Involved in the Biosynthesis of Lipopeptide Biosurfactants
Niran Roongsawang, Kenji Washio, Masaaki Morikawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 12, 1, 141, 172, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Analyses of three dominant membrane proteins from anammox planctomycete Candidatus ‘Brocadia sinica’
Tojo F, Itoh Y, Okabe S, Morikawa M
J. Environ. Biotechnol., 11, 1-2, 77, 81, 環境バイオテクノロジー学会, 2011, [Peer-reviewed], [Corresponding author]
English

Dioxygen activation responsible for oxidation of aliphatic and aromatic hydrocarbon compounds: current state and variants
Masaaki Morikawa
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87, 5, 1595, 1603, Aug. 2010, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Sustainable Biodegradation of Phenol by Acinetobacter calcoaceticus P23 Isolated from the Rhizosphere of Duckweed Lemna aoukikusa
Fumiko Yamaga, Kenji Washio, Masaaki Morikawa
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 44, 16, 6470, 6474, Aug. 2010, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Identification and Characterization of the Genes Responsible for the Production of the Cyclic Lipopeptide Arthrofactin by Pseudomonas sp MIS38
Kenji Washio, Siew Ping Lim, Niran Roongsawang, Masaaki Morikawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74, 5, 992, 999, May 2010, [Peer-reviewed]
English, Scientific journal

The Role of Urease Activity on Biofilm Formation by Staphylococcus sp T-02 Isolated from the Toilet Bowl
Kaihei Oki, Kenji Washio, Daigo Matsui, Shinichi Kato, Yoshihiko Hirata, Masaaki Morikawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74, 3, 583, 589, Mar. 2010, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Feasibility of bioremediation technologies for managing oil spills caused by the development of the Sakhalin oil fields-Review-
Sakaguchi Naofumi, Bin Haji Mohd Taha Ahmad Iskandar, Maki Hideaki, Hamada Seiichi, Morikawa Masaaki, Okuyama Hidetoshi
生物工学会誌, 88, 4, 150, 157, 日本生物工学会, 2010, [Peer-reviewed]
English, The oil fields at Sakhalin Island, Russia, reached a high level of operation in 2008. The Japanese island of Hokkaido is located to the south of Sakhalin, and is at risk of oil spills following explosions at the Sakhalin oil fields and from accidents involving pipelines and tankers at the fields and in the surrounding seas. Physical, chemical, and biological treatments are indispensable in cleaning up any contaminated regions, such as seas and seashores. In this review, we describe various types of accidents, particularly those involving tanker oil spills around the world and the bioremediation (BR) treatment employed. We emphasize the usefulness of BR in colder regions, such as Hokkaido. Pioneering on-site biostimulation (BS) testing has been performed on the Hokkaido coast with the aim of dealing with oil contamination caused by oil spills at Sakhalin. In the BS process, appropriate nutrients other than carbon sources are supplied to contaminated soils or waters to activate native degraders of the contaminants. In another bioaugmentation (BA) process, isolated microorganisms and their mixtures can be used as degraders of xenobiotics. We proposed and tested a new bioremediation technology, autochthonous bioaugmentation (ABA), in which contaminant-degrading microorganisms indigenous to the contaminated site or predicted contamination site are isolated and used. The feasibility of applying ABA to oil spills caused by the development of the Sakhalin oil fields is discussed.

Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23
Tomohisa Kato, Asuka Miyanaga, Shigenori Kanaya, Masaaki Morikawa
EXTREMOPHILES, 14, 1, 33, 39, Jan. 2010, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Autochthonous bioaugmentation and its possible application to oil spills
Reia Hosokawa, Motonori Nagai, Masaaki Morikawa, Hidetoshi Okuyama
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 25, 9, 1519, 1528, Sep. 2009, [Peer-reviewed]
English

Flexible exportation mechanisms of arthrofactin in Pseudomonas sp MIS38
S. P. Lim, N. Roongsawang, K. Washio, M. Morikawa
JOURNAL OF APPLIED MICROBIOLOGY, 107, 1, 157, 166, Jul. 2009, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Biofilm formation and proteolytic activities of Pseudoalteromonas bacteria that were isolated from fish farm sediments
Saori Iijima, Kenji Washio, Ryota Okahara, Masaaki Morikawa
MICROBIAL BIOTECHNOLOGY, 2, 3, 361, 369, May 2009, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Alkane inducible proteins in Geobacillus thermoleovorans B23
Tomohisa Kato, Asuka Miyanaga, Shigenori Kanaya, Masaaki Morikawa
BMC MICROBIOLOGY, 9, 60, Mar. 2009, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Development of bioremediation by utilizing biofilms.　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
IFO Res. Commun., 23, 99, 115, 2009, [Peer-reviewed]
English

Identification of alkane hydroxylase genes in Rhodococcus sp strain TMP2 that degrades a branched alkane
Daisuke Takei, Kenji Washio, Masaaki Morikawa
BIOTECHNOLOGY LETTERS, 30, 8, 1447, 1452, Aug. 2008, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Production of sophorolipid biosurfactant by Pichia anomala
Jiraporn Thaniyavarn, Tanusta Chianguthai, Polakit Sangvanich, Niran Roongsawang, Keji Washio, Masaaki Morikawa, Suthep Thaniyavarn
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 72, 8, 2061, 2068, Aug. 2008, [Peer-reviewed]
English, Scientific journal

A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics
Hitoshi Ito, Reia Hosokawa, Masaaki Morikawa, Hidetoshi Okuyama
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 61, 3, 223, 232, Apr. 2008, [Peer-reviewed]
English, Scientific journal

Duckweed-Rhizobacteria Symbiosis that Develops Sustainable Remediation Technology
YAMAGA Fumiko, WASHIO Kenji, MORIKAWA Masaaki
KAGAKU TO SEIBUTSU, 46, 10, 682, 688, Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2008, [Peer-reviewed]
Japanese, 微生物活性を利用した環境浄化法は，地球への負荷が少ない技術として広く認知されている．しかし，実用的技術としては，有用微生物の現場での定着率の低さによるコスト高や浄化後に危惧される微生物の異常増殖や生態系の攪乱などの問題が常に指摘されてきた．近年，この問題を解決する一つの方向性として根圏微生物の活用が注目されている．植物の光合成作用と微生物の汚染物質分解活性をリンクさせるこの手法の一例を紹介するとともに，今後の可能性を展望する．

Cloning of hyaluronan synthase (sz-hos) gene from Streptococcus zooepidemicus in Escherichia coli
Boonsri Jongsareejit, Amaret Bhumiratana, Masaaki Morikawa, Shigerri Kanaya
ScienceAsia, 33, 4, 389, 395, Dec. 2007, [Peer-reviewed]
English, Scientific journal

Functional analysis of a pyoverdine synthetase from Pseudomonas sp MIS38
Slew Ping Lim, Niran Roongsawang, Kenji Washio, Masaaki Morikawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 71, 8, 2002, 2009, Aug. 2007, [Peer-reviewed], [Corresponding author]
English, Scientific journal

In vivo characterization of tandem C-terminal thioesterase domains in arthrofactin synthetase
Niran Roongsawang, Kenji Washio, Masaaki Morikawa
CHEMBIOCHEM, 8, 5, 501, 512, Mar. 2007, [Peer-reviewed], [Corresponding author]
English, Scientific journal

バイオフィルムの功罪　　　　　　　　　　　　　　　
森川 正章
食品衛生学雑誌, 48, J389, J396, 2007, [Peer-reviewed]
Japanese

Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W
Shin-ichi Hirano, Masaaki Morikawa, Kazufumi Takano, Tadayuki Imanaka, Shigenori Kanaya
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 71, 1, 192, 199, Jan. 2007, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Common mechanisms regulating expression of rice aleurone genes that contribute to the primary response for gibberellin
Kenji Washio, Masaaki Morikawa
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1759, 10, 478, 490, Oct. 2006, [Peer-reviewed]
English, Scientific journal

Biofilm formation by a Bacillus subtilis strain that produces gamma-polyglutamate
Masaaki Morikawa, Shinji Kagihiro, Mitsuru Haruki, Kazufumi Takano, Steve Branda, Roberto Kolter, Shigenori Kanaya
MICROBIOLOGY-SGM, 152, 9, 2801, 2807, Sep. 2006, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Biosurfactant production by Pseudomonas aeruginosa A41 using palm oil as carbon source
Jiraporn Thaniyavarn, Aree Chongchin, Nopparat Wanitsuksombut, Suthep Thaniyavarn, Pairoh Pinphanichakarn, Natthanant Leepipatpiboon, Masaaki Morikawa, Shigenori Kanaya
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 52, 4, 215, 222, Aug. 2006, [Peer-reviewed]
English, Scientific journal

Ca2+-dependent maturation of subtilisin from a hyperthermophilic archaeon, Thermococcus kodakaraensis: the propeptide is a potent inhibitor of the mature domain but is not required for its folding
M Pulido, K Saito, SI Tanaka, Y Koga, M Morikawa, K Takano, S Kanaya
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72, 6, 4154, 4162, Jun. 2006, [Peer-reviewed]
English, Scientific journal

Gene cloning and in vivo characterization of a dibenzothiophene dioxygenase from Xanthobacter polyaromaticivorans
S Hirano, M Haruki, K Takano, T Imanaka, M Morikawa, S Kanaya
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 69, 6, 672, 681, Feb. 2006, [Peer-reviewed], [Corresponding author]
English, Scientific journal

:Learning from the Microbial Activities which Exceed Central Dogma
WASHIO Kenji, MORIKAWA Masaaki
KAGAKU TO SEIBUTSU, 44, 2, 85, 92, Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2006, [Peer-reviewed]
Japanese

枯草菌バイオフィルム形成遺伝子の探索
森川正章, 河合良尚, 相原 悠, 鷲尾健司, 高野和文, 金谷茂則
生物工学会誌, 84, 12, 488, 490, 日本生物工学会, 2006, [Peer-reviewed]
Japanese

Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA
T Yamamoto, T Matsuda, T Inoue, H Matsumura, M Morikawa, S Kanaya, Y Kai
PROTEIN SCIENCE, 15, 1, 152, 161, Jan. 2006, [Peer-reviewed]
English, Scientific journal

Beneficial biofilm formation by industrial bacteria Bacillus subtilis and related species
M Morikawa
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 101, 1, 1, 8, Jan. 2006, [Peer-reviewed]
English

Phylogenetic analysis of condensation domains in the nonribosomal peptide synthetases
N Roongsawang, SP Lim, K Washio, K Takano, S Kanaya, M Morikawa
FEMS MICROBIOLOGY LETTERS, 252, 1, 143, 151, Nov. 2005, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Gene cloning, overproduction, and characterization of thermolabile alkaline phosphatase from a psychrotrophic bacterium
Y Suzuki, Y Mizutani, T Tsuji, N Ohtani, K Takano, M Haruki, M Morikawa, S Kanaya
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 69, 2, 364, 373, Feb. 2005, [Peer-reviewed]
English, Scientific journal

Isolation and characterization of Rhodococcus sp strains TNP2 and T12 that degrade 2,6,10,14-tetramethylpentadecane (pristane) at moderately low temperatures
N Kunihiro, M Haruki, K Takano, M Morikawa, S Kanaya
JOURNAL OF BIOTECHNOLOGY, 115, 2, 129, 136, Jan. 2005, [Peer-reviewed]
English, Scientific journal

Comparison of dibenzothiophene-degrading bacteria under low oxygen conditions
Kitauchi F, Hirano S, Haruki M, Imanaka T, Morikawa M, Kanaya S
J. Environ. Biotechnol., 4, 2, 117, 120, 環境バイオテクノロジー学会, 2005, [Peer-reviewed]
English

Structure of RadB recombinase from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1: an implication for the formation of a near-7-fold helical assembly.
Toshihiko Akiba, Noriyuki Ishii, Naeem Rashid, Masaaki Morikawa, Tadayuki Imanaka, Kazuaki Harata
Nucleic acids research, 33, 10, 3412, 23, 2005, [Peer-reviewed], [International Magazine]
English, The X-ray crystal structure of RadB from Thermococcus kodakaraensis KOD1, an archaeal homologue of the RecA/Rad51 family proteins, have been determined in two crystal forms. The structure represents the core ATPase domain of the RecA/Rad51 proteins. Two independent molecules in the type 1 crystal were roughly related by 7-fold screw symmetry whereas non-crystallographic 2-fold symmetry was observed in the type 2 crystal. The dimer structure in the type 1 crystal is extended to construct a helical assembly, which resembles the filamentous structures reported for other RecA/Rad51 proteins. The molecular interface in the type 1 dimer is formed by facing a basic surface patch of one monomer to an acidic one of the other. The empty ATP binding pocket is located at the interface and barely concealed from the outside similarly to that in the active form of the RecA filament. The model assembly has a positively charged belt on one surface bordering the helical groove suitable for facile binding of DNA. Electron microscopy has revealed that, in the absence of ATP and DNA, RadB forms a filament with a similar diameter to that of the hypothetical assembly, although its helical properties were not confirmed.

Kinetically robust monomeric protein from a hyperthermophile
A Mukaiyama, K Takano, M Haruki, M Morikawa, S Kanaya
BIOCHEMISTRY, 43, 43, 13859, 13866, Nov. 2004, [Peer-reviewed]
English, Scientific journal

Cleavage of various peptides with pitrilysin from Escherichia coli: Kinetic analyses using beta-endorphin and its derivatives
J Cornista, S Ikeuchi, M Haruki, A Kohara, K Takano, M Morikawa, S Kanaya
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68, 10, 2128, 2137, Oct. 2004, [Peer-reviewed]
English, Scientific journal

Gene cloning and biochemical characterizations of thermostable ribonuclease HIII from Bacillus stearothermophilus
H Chon, R Nakano, N Ohtani, M Haruki, K Takano, M Morikawa, S Kanaya
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68, 10, 2138, 2147, Oct. 2004, [Peer-reviewed]
English, Scientific journal

Mutational and structural-based analyses of the osmolyte effect on protein stability
K Takano, M Saito, M Morikawa, S Kanaya
JOURNAL OF BIOCHEMISTRY, 135, 6, 701, 708, Jun. 2004, [Peer-reviewed]
English, Scientific journal

Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp SIB1 in cold-adaptation
Y Suzuki, M Haruki, K Takano, M Morikawa, S Kanaya
EUROPEAN JOURNAL OF BIOCHEMISTRY, 271, 7, 1372, 1381, Apr. 2004, [Peer-reviewed]
English, Scientific journal

Isolation and characterization of Xanthobacter polyaromaticivorans sp nov 127W that degrades polycyclic and heterocyclic aromatic compounds under extremely low oxygen conditions
SI Hirano, F Kitauchi, M Haruki, T Imanaka, M Morikawa, S Kanaya
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68, 3, 557, 564, Mar. 2004, [Peer-reviewed]
English, Scientific journal

次世代のペプチド合成法　　　　　　　　　　　　　　　
森川正章, 金谷茂則
日本化学会バイオテクノロジー部会ニュースレター, 8, 5, 7, 2004, [Peer-reviewed]
Japanese

Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reported as Pyrococcus sp. KOD1
Haruyuki Atomi, Toshiaki Fukui, Tamotsu Kanai, Masaaki Morikawa, Tadayuki Imanaka
Archaea, 1, 4, 263, 267, Heron Publishing, 2004, [Peer-reviewed]
English, Scientific journal

Cloning and characterization of the gene cluster encoding arthrofactin synthetase from Pseudomonas sp MIS38
N Roongsawang, K Hase, M Haruki, T Imanaka, M Morikawa, S Kanaya
CHEMISTRY & BIOLOGY, 10, 9, 869, 880, Sep. 2003, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Production and characterization of biosurfactants from Bacillus licheniformis F2.2
J Thaniyavarn, N Roongsawang, T Kameyama, M Haruki, T Imanaka, M Morikawa, S Kanaya
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 67, 6, 1239, 1244, Jun. 2003, [Peer-reviewed]
English, Scientific journal

Dispensability of glutamic acid 48 and aspartic acid 134 for Mn2+-dependent activity of Escherichia coli ribonuclease HI
Y Tsunaka, M Haruki, M Morikawa, M Oobatake, S Kanaya
BIOCHEMISTRY, 42, 11, 3366, 3374, Mar. 2003, [Peer-reviewed]
English, Scientific journal

Crystallization and preliminary X-ray analysis of TBP-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1
T Yamamoto, T Matsuda, N Sakamoto, H Matsumura, T Inoue, M Morikawa, S Kanaya, Y Kai
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 59, 2, 372, 374, Feb. 2003, [Peer-reviewed]
English, Scientific journal

掘り起こせ宝の山−微生物のしたたかな戦略バイオフィルム　　　　　　　　　　　　　　　
森川 正章
バイオサイエンスとインダストリー, 61, 2003, [Peer-reviewed]
Japanese

Biofilms-Even Microorganisms Live Together
MORIKAWA Masaaki
KAGAKU TO SEIBUTSU, 41, 1, 32, 37, Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2003, [Peer-reviewed]
Japanese

Application of a two-liquid system to sitting-drop vapour-diffusion protein crystallization
H Adachi, K Takano, M Morikawa, S Kanaya, M Yoshimura, Y Mori, T Sasaki
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 59, Pt1, 194, 196, Jan. 2003, [Peer-reviewed]
English, Scientific journal

Laser irradiated growth of protein crystal.
Adachi H, Takano K, Hosokawa Y, Inoue T, Mori Y, Matsumura H, Yoshimura M, Tsunaka Y, Morikawa M, Kanaya S, Masuhara H, Kai Y, Sasaki T
Jpn. J. Appl. Phys., 42, 7B, L798, L800, INSTITUTE OF PURE AND APPLIED PHYSICS, 2003, [Peer-reviewed]
English, We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).

Stability of Ribonuclease HII from Hyperthermophile, Thermococcus kod akaraensis
Mukaiyama A., Takano K., Haruki M., Morikawa M., Kanaya S.
Seibutsu Butsuri, 43, S68, The Biophysical Society of Japan General Incorporated Association, 2003
Japanese

Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin
N Roongsawang, J Thaniyavarn, S Thaniyavarn, T Kameyama, M Haruki, T Imanaka, M Morikawa, S Kanaya
EXTREMOPHILES, 6, 6, 499, 506, Dec. 2002, [Peer-reviewed]
English, Scientific journal

Oleomonas sagaranensis gen. nov., sp nov., represents a novel genus in the alpha-Proteobacteria
T Kanamori, N Rashid, M Morikawa, H Atomi, T Imanaka
FEMS MICROBIOLOGY LETTERS, 217, 2, 255, 261, Dec. 2002, [Peer-reviewed]
English, Scientific journal

Cleavage of a DNA-RNA-DNA/DNA chimeric substrate containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII
M Haruki, Y Tsunaka, M Morikawa, S Kanaya
FEBS LETTERS, 531, 2, 204, 208, Nov. 2002, [Peer-reviewed]
English, Scientific journal

Site-specific cleavage of MS2 RNA by a thermostable DNA-linked RNase H
H Chon, Y Tsunaka, M Haruki, M Morikawa, S Kanaya
PROTEIN ENGINEERING, 15, 8, 683, 688, Aug. 2002, [Peer-reviewed]
English, Scientific journal

Role of repetitive nine-residue sequence motifs in secretion, enzymatic activity, and protein conformation of a family I.3 lipase
HJ Kwon, M Haruki, M Morikawa, K Omori, S Kanaya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93, 2, 157, 164, Feb. 2002, [Peer-reviewed]
English, Scientific journal

Importance of an N-terminal extension in ribonuclease HII from Bacillus stearothermophilus for substrate binding
A Muroya, R Nakano, N Ohtani, M Haruki, M Morikawa, S Kanaya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93, 2, 170, 175, Feb. 2002, [Peer-reviewed]
English, Scientific journal

特殊環境微生物の環境修復への応用と限界　　　　　　　　　　　　　　　
森川 正章
創造と実践—大阪大学全学共通教育機構—, 2, 66, 71, 2002, [Peer-reviewed]
Japanese

Heat labile ribonuclease HI from a psychrotrophic bacterium: gene cloning, characterization and site-directed mutagenesis
N Ohtani, M Haruki, M Morikawa, S Kanaya
PROTEIN ENGINEERING, 14, 12, 975, 982, Dec. 2001, [Peer-reviewed]
English, Scientific journal

Ca2+-induced folding of a family 1.3 lipase with repetitive Ca2+ binding motifs at the C-terminus
K Amada, HJ Kwon, M Haruki, M Morikawa, S Kanaya
FEBS LETTERS, 509, 1, 17, 21, Nov. 2001, [Peer-reviewed]
English, Scientific journal

Isolation and characterization of psychrotrophic bacteria from oil-reservoir water and oil sands
T Kato, M Haruki, T Imanaka, M Morikawa, S Kanaya
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 55, 6, 794, 800, Jun. 2001, [Peer-reviewed]
English, Scientific journal

Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence
Y Kannan, Y Koga, Y Inoue, M Haruki, M Takagi, T Imanaka, M Morikawa, S Kanaya
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67, 6, 2445, 2452, Jun. 2001, [Peer-reviewed]
English, Scientific journal

Interaction of TIP26 from a hyperthermophilic archaeon with TFB/TBP/DNA ternary complex
T Matsuda, R Fujikawa, M Haruki, XF Tang, S Ezaki, T Imanaka, M Morikawa, S Kanaya
EXTREMOPHILES, 5, 3, 177, 182, Jun. 2001, [Peer-reviewed]
English, Scientific journal

Stabilities of chimeras of hyperthermophilic and mesophilic glycerol kinases constructed by DNA shuffling
Y Koga, M Haruki, M Morikawa, S Kanaya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91, 6, 551, 556, Jun. 2001, [Peer-reviewed]
English, Scientific journal

Erratum - Identification of the histidine and aspartic acid residues essential for enzymatic activity of a family I.3 lipase by site-directed mutagenesis (FEBS Letters (2000) 483 (139-142) PII: S0014579300021037)
H. J. Kwon, K. Amada, M. Haruki, M. Morikawa, S. Kanaya
FEBS Letters, 497, 2-3, 174, 25 May 2001, [Peer-reviewed]
English, Scientific journal

Strong nucleic acid binding to the Escherichia coli RNase HI mutant with two arginine residues at the active site
Y Tsunaka, M Haruki, M Morikawa, S Kanaya
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1547, 1, 135, 142, May 2001, [Peer-reviewed]
English, Scientific journal

Catalytic center of an archaeal type 2 ribonuclease H as revealed by X-ray crystallographic and mutational analyses
A Muroya, D Tsuchiya, M Ishikawa, M Haruki, M Morikawa, S Kanaya, K Morikawa
PROTEIN SCIENCE, 10, 4, 707, 714, Apr. 2001, [Peer-reviewed]
English, Scientific journal

バイオレメディエーション　　　　　　　　　　　　　　　
森川 正章
学研ハイベスト教科事典「生物と環境」, 223, 2001, [Peer-reviewed]
Japanese

Thiol protease from Thermococcus kodakaraensis KOD1
M Morikawa, T Imanaka
HYPERTHERMOPHILIC ENZYMES, PT A, 330, 424, 433, 2001, [Peer-reviewed]
English

Isolation and characterization of long-chain-alkane degrading Bacillus thermoleovorans from deep subterranean petroleum reservoirs
T Kato, M Haruki, T Imanaka, M Morikawa, S Kanaya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91, 1, 64, 70, Jan. 2001, [Peer-reviewed]
English, Scientific journal

Gene cloning of an alcohol dehydrogenase from thermophilic alkane-degrading Bacillus thermoleovorans B23
T Kato, A Miyanaga, M Haruki, T Imanaka, M Morikawa, S Kanaya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91, 1, 100, 102, Jan. 2001, [Peer-reviewed]
English, Scientific journal

RecA/Rad51 homolog from Thermococcus kodakaraensis KOD1
N Rashid, M Morikawa, S Kanaya, H Atomi, T Imanaka
HYPERTHERMOPHILIC ENZYMES, PT C, 334, 261, 270, 2001, [Peer-reviewed]
English

Efficient cleavage of RNA at high temperatures by a thermostable DNA-linked ribonuclease H
M Haruki, T Nogawa, N Hirano, H Chon, Y Tsunaka, M Morikawa, S Kanaya
PROTEIN ENGINEERING, 13, 12, 881, 886, Dec. 2000, [Peer-reviewed]
English, Scientific journal

Catalysis by Escherichia coli ribonuclease HI is facilitated by a phosphate group of the substrate
M Haruki, Y Tsunaka, M Morikawa, S Iwai, S Kanaya
BIOCHEMISTRY, 39, 45, 13939, 13944, Nov. 2000, [Peer-reviewed]
English, Scientific journal

A study on the structure-function relationship of lipopeptide biosurfactants
M Morikawa, Y Hirata, T Imanaka
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1488, 3, 211, 218, Nov. 2000, [Peer-reviewed]
English, Scientific journal

Enhancement of the enzymatic activity of ribonuclease HI from Thermus thermophilus HB8 with a suppressor mutation method
N Hirano, M Haruki, M Morikawa, S Kanaya
BIOCHEMISTRY, 39, 43, 13285, 13294, Oct. 2000, [Peer-reviewed]
English, Scientific journal

Identification of the histidine and aspartic acid residues essential for enzymatic activity of a family I.3 lipase by site-directed mutagenesis
K Hyun-Ju, K Amada, M Haruki, M Morikawa, S Kanaya
FEBS LETTERS, 483, 2-3, 139, 142, Oct. 2000, [Peer-reviewed]
English, Scientific journal

Overproduction in Escherichia coli, purification and characterization of a family I.3 lipase from Pseudomonas sp MIS38
K Amada, M Haruki, T Imanaka, M Morikawa, S Kanaya
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1478, 2, 201, 210, May 2000, [Peer-reviewed]
English, Scientific journal

Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form
N Ohtani, M Haruki, A Muroya, M Morikawa, S Kanaya
JOURNAL OF BIOCHEMISTRY, 127, 5, 895, 899, May 2000, [Peer-reviewed]
English, Scientific journal

Thermophilic bacteria isolated from a subterranean petroleum reservoir.　　　　　　　　　　　　　　　
M. Morikawa, T. Kato, M. Haruki, T. Imanaka, S. Kanaya
In Gratama Workshop 2000 conference report, 202, 203, 2000, [Peer-reviewed]
English

Decarbonylase, an enzyme which transforms aldehyde to alkene.　　　　　　　　　　　　　　　
H. Takagi, M. Haruki, N. Murakami, T. Imanaka, M. Morikawa, S. Kanaya
In Proceedings of Fifth International Symposium on Environmental Biotechnology, 2000, [Peer-reviewed]
English

Aldehyde dehydrogenase and esterase from a petroleum-degrading bacterium HD-1.　　　　　　　　　　　　　　　
S. Kanaya, N. Okibe, S. Mizuguchi, M. Haruki, T. Imanaka, M. Morikawa
In Proceedings of Fifth International Symposium on Environmental Biotechnology, 2000, [Peer-reviewed]
English

An extremely thermophile which degrades petroleum under high termperature conditions.　　　　　　　　　　　　　　　
T. Kato, A. Miyanaga, M. Haruki, T. Imanaka, M. Morikawa, S. Kanaya
In Proceedings of Fifth International Symposium on Environmental Biotechnology, 2000, [Peer-reviewed]
English

Enzymes from a psychrophilic Shewanella sp. SIB1.　　　　　　　　　　　　　　　
M. Haruki, N. Ohtani, K. Miura, Y. Suzuki, T. Kato, M. Morikawa, S. Kanaya
In Proceedings of Fifth International Symposium on Environmental Biotechnology, 2000, [Peer-reviewed]
English

Petroleum Degradation by an extremely thermophilic bacteria.　　　　　　　　　　　　　　　
M. Morikawa, T. Kato, A. Miyanaga, M. Haruki, T. Imanaka, S. Kanaya
In Proceedings of Fifth International Symposium on Environmental Biotechnology, 2000, [Peer-reviewed]
English

新規抗菌物質の探索　　　　　　　　　　　　　　　
森川正章, 金谷茂則, 藤本善久
大阪大学先端科学技術共同研究センター年報, 6, 18, 20, 2000, [Peer-reviewed]
Japanese

微生物にもとめる火事場の．．．？　　　　　　　　　　　　　　　
森川 正章
The News of Engineering. Osaka University., 9, 2000, [Peer-reviewed]
Japanese

Crystallization and preliminary X-ray study of Pk-REC from a hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1
Kazuaki Harata, Noriyuki Ishii, Naeem Rashid, Masaaki Morikawa, Tadayuki Imanaka
Acta Crystallographica Section D: Biological Crystallography, 56, 5, 648, 649, 2000, [Peer-reviewed]
English, Scientific journal

Identification of the gene encoding esterase, a homolog of hormone-sensitive lipase, from an oil-degrading bacterium, strain HD-1
S Mizuguchi, K Amada, M Haruki, T Imanaka, M Morikawa, S Kanaya
JOURNAL OF BIOCHEMISTRY, 126, 4, 731, 737, Oct. 1999, [Peer-reviewed]
English, Scientific journal

Isolation of TBP-interacting protein (TIP) from a hyperthermophilic archaeon that inhibits the binding of TBP to TATA-DNA
T Matsuda, M Morikawa, M Haruki, H Higashibata, T Imanaka, S Kanaya
FEBS LETTERS, 457, 1, 38, 42, Aug. 1999, [Peer-reviewed]
English, Scientific journal

Molecular diversities of RNases H
N Ohtani, M Haruki, M Morikawa, S Kanaya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 88, 1, 12, 19, Jul. 1999, [Peer-reviewed]
English, Scientific journal

Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam
NQ Huy, S Jin, K Amada, M Haruki, NB Huu, DT Hang, DTC Ha, T Imanaka, M Morikawa, S Kanaya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 88, 1, 100, 102, Jul. 1999, [Peer-reviewed]
English, Scientific journal

Identification of catalytically essential residues in Escherichia coli esterase by site-directed mutagenesis
M Haruki, Y Oohashi, S Mizuguchi, Y Matsuo, M Morikawa, S Kanaya
FEBS LETTERS, 454, 3, 262, 266, Jul. 1999, [Peer-reviewed]
English, Scientific journal

A unique DNase activity shares the active site with ATPase activity of the RecA/Rad51 homologue (Pk-REC) from a hyperthermophilic archaeon
N Rashid, M Morikawa, S Kanaya, H Atomi, T Imanaka
FEBS LETTERS, 445, 1, 111, 114, Feb. 1999, [Peer-reviewed]
English, Scientific journal

環境問題を大学で学ぶ　　　　　　　　　　　　　　　
森川 正章
栄冠をめざしてSPECIAL．河合塾／全国進学情報センター, 特別, 1999, [Peer-reviewed]
Japanese

CO2固定細菌を利用した地球環境修復システムの構築
森川 正章
生産技術, 51, 1, 153, 157, 生産技術振興協会, 1999, [Peer-reviewed]
Japanese

Identification of the genes encoding Mn2+-dependent RNase I-III and Mg2+-dependent RNase HIII from Bacillus subtilis: Classification of RNases H into three families
N Ohtani, M Haruki, M Morikawa, RJ Crouch, M Itaya, S Kanaya
BIOCHEMISTRY, 38, 2, 605, 618, Jan. 1999, [Peer-reviewed]
English, Scientific journal

Gene cloning and characterization of aldehyde dehydrogenase from a petroleum-degrading bacterium, strain HD-1
Naoko Okibe, Kei Amada, Shin Ichi Hirano, Mitsuru Haruki, Tadayuki Imanaka, Masaaki Morikawa, Shigenori Kanaya
Journal of Bioscience and Bioengineering, 88, 1, 7, 11, 1999, [Peer-reviewed]
English, Scientific journal

Thermostable glycerol kinase from a hyperthermophilic archaeon: gene cloning and characterization of the recombinant enzyme
Y Koga, M Morikawa, M Haruki, H Nakamura, T Imanaka, S Kanaya
PROTEIN ENGINEERING, 11, 12, 1219, 1227, Dec. 1998, [Peer-reviewed]
English, Scientific journal

Gene cloning and characterization of recombinant RNase HII from a hyperthermophilic archaeon
M Haruki, K Hayashi, T Kochi, A Muroya, Y Koga, M Morikawa, T Imanaka, S Kanaya
JOURNAL OF BACTERIOLOGY, 180, 23, 6207, 6214, Dec. 1998, [Peer-reviewed]
English, Scientific journal

Cloning of a ribonuclease HII gene from a hyperthermophilic archaeon: Purification and characterization of a recombinant enzyme.　　　　　　　　　　　　　　　
Haruki, M, Hayashi, K, Kouchi, T, Muroya, A, Koga, Y, Morikawa, M, Imanaka, T, Kanaya, S
J. Bacteriol., Dec. 1998, [Peer-reviewed]
English, Scientific journal

Stabilization of ribonuclease HI from Thermus thermophilus HB8 by the spontaneous formation of an intramolecular disulfide bond
N Hirano, M Haruki, M Morikawa, S Kanaya
BIOCHEMISTRY, 37, 36, 12640, 12648, Sep. 1998, [Peer-reviewed]
English, Scientific journal

Production of alkane and alkene from CO2 by a petroleum-degrading bacterium strain HD-1
M Morikawa, T Iwasa, K Nagahisa, S Yanagida, T Imanaka
ADVANCES IN CHEMICAL CONVERSIONS FOR MITIGATING CARBON DIOXIDE, 114, 467, 470, 1998, [Peer-reviewed]
English, Scientific journal

炭酸ガスからの石油生成
森川正章, 金 沙, 金谷茂則, 今中忠行
農芸化学会誌, 72, 4, 532, 534, 1998, [Peer-reviewed]
Japanese

大学推進型新プロジェクトが創る夢　　　　　　　　　　　　　　　
森川 正章
大阪大学工業会誌テクノネット, 501, 9, 1998, [Peer-reviewed]
Japanese

Production of alkane and alkene from CO2 by a petroleum-degrading bacterium
M Morikawa, T Iwasa, S Yanagida, T Imanaka
JOURNAL OF FERMENTATION AND BIOENGINEERING, 85, 2, 243, 245, 1998, [Peer-reviewed]
English, Scientific journal

Characterization of a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcus sp KOD1
N Rashid, M Morikawa, K Nagahisa, S Kanaya, T Imanaka
NUCLEIC ACIDS RESEARCH, 25, 4, 719, 726, Feb. 1997, [Peer-reviewed]
English, Scientific journal

A new type of petroleum-degrading/producing bacterium HD-1.　　　　　　　　　　　　　　　
M. Morikawa, T. Imanaka
In Proceedings of International Workshop on Ultra-Long-Term Cryogenic, 51, 60, 1997, [Peer-reviewed]
English

Gene cloning and characterization of recombinant ribonuclease HII from a hyperthermophilic archaeon.　　　　　　　　　　　　　　　
Haruki M, Hayashi K, Kochi T, Muroya A, Koga Y, Morikawa M, Imanaka T, Kanaya S
Annual reports of ICBiotech., 20, 803, 821, 1997, [Peer-reviewed]
English

超好熱菌の新機能解析と耐熱酵素に関する研究　　　　　　　　　　　　　　　
高木昌宏, 金谷茂則, 森川正章, 春木 満, 藤原伸介, 橘 佳永
大阪大学先端科学共同研究センター年報, 2, 35, 38, 1997, [Peer-reviewed]
Japanese

Gene cloning and characterization of recombinant ribose phosphate pyrophosphokinase from a hyperthermophilic archaeon
N Rashid, M Morikawa, T Imanaka
JOURNAL OF FERMENTATION AND BIOENGINEERING, 83, 5, 412, 418, 1997, [Peer-reviewed]
English, Scientific journal

Anaerobic degradation and production of alkane/alkene by a new facultative chemoautotrophic bacterium strain HD-1
T Imanaka, M Morikawa
GLOBAL ENVIRONMENTAL BIOTECHNOLOGY, 401, 414, 1997, [Peer-reviewed]
English, International conference proceedings

Isolation and characterisation of a new lipopeptide biosurfactant produced by Arthrobacter sp MIS38
M Morikawa, T Imanaka
GLOBAL ENVIRONMENTAL BIOTECHNOLOGY, 585, 596, 1997, [Peer-reviewed]
English, International conference proceedings

A RecA/RAD51 homologue from a hyperthermophilic archaeon retains the major RecA domain only
N Rashid, M Morikawa, T Imanaka
MOLECULAR & GENERAL GENETICS, 253, 3, 397, 400, Dec. 1996, [Peer-reviewed]
English, Scientific journal

A new mixotrophic bacterium that can fix CO2 and assimilate aliphatic and aromatic hydrocarbons anaerobically
T Imanaka, M Morikawa
MOLECULAR BIOLOGY OF PSEUDOMONADS, 289, 297, 1996, [Peer-reviewed]
English, International conference proceedings

RecA/RAD51 homologue from a hyperthermophilic archaeon maintains the major domain only.　　　　　　　　　　　　　　　
Rashid N, Morikawa M, Imanaka T
Annual reports of ICBiotech., 19, 713, 718, 1996, [Peer-reviewed]
English

微生物が人類と地球を救う
森川正章, 今中忠行
生産技術, 48, 4, 59, 62, 生産技術振興協会, 1996, [Peer-reviewed]
Japanese

Biological oxidation of alkane to alkene under anaerobic conditions
M Morikawa, M Kanemoto, T Imanaka
JOURNAL OF FERMENTATION AND BIOENGINEERING, 82, 3, 309, 311, 1996, [Peer-reviewed]
English, Scientific journal

Isolation of a new mixotrophic bacterium which can fix CO2 and assimilate aliphatic and aromatic hydrocarbons anaerobically
T Imanaka, M Morikawa
ENVIRONMENTAL BIOTECHNOLOGY, 16, 27, 1996, [Peer-reviewed]
English, International conference proceedings

AN ABNORMALLY ACIDIC TATA-BINDING PROTEIN FROM A HYPERTHERMOPHILIC ARCHAEON
N RASHID, M MORIKAWA, T IMANAKA
GENE, 166, 1, 139, 143, Dec. 1995, [Peer-reviewed]
English

バイオサーファクタント実用化の試み　　　　　　　　　　　　　　　
今中忠行, 平田善彦, 森川正章
Rep. Chem. Mat. R & D Found., 10, 103, 106, 1995, [Peer-reviewed]
Japanese

石油をつくる細菌発見
森川正章, 今中忠行
高圧ガス, 32, 4, 336, 339, 高圧ガス保安協会, 1995, [Peer-reviewed]
Japanese

石油を作る細菌
今中忠行, 森川正章
燃料及燃焼, 62, 5, 323, 330, 燃料及燃焼社, 1995, [Peer-reviewed]
Japanese

超好熱菌由来チオールプロテアーゼ耐熱性獲得機構の解析
森川正章
日本農芸化学会誌, 69, 6, 756, 757, 1995, [Peer-reviewed]
Japanese

微生物による石油生産-脂肪族炭化水素の生合成経路について
森川正章, 今中忠行
蛋白質 核酸 酵素, 40, 1, 52, 60, 共立出版, 1995, [Peer-reviewed]
English

GENE CLONING AND CHARACTERIZATION OF THERMOSTABLE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE (PPIASE) FROM BACILLUS-STEAROTHERMOPHILUS SIC1
DJ KIM, M MORIKAWA, M TAKAGI, T IMANAKA
JOURNAL OF FERMENTATION AND BIOENGINEERING, 79, 2, 87, 94, 1995, [Peer-reviewed]
English, Scientific journal

PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE THIOL PROTEASE FROM A NEWLY ISOLATED HYPERTHERMOPHILIC PYROCOCCUS SP
M MORIKAWA, Y IZAWA, N RASHID, T HOAKI, T IMANAKA
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 60, 12, 4559, 4566, Dec. 1994, [Peer-reviewed]
English, Scientific journal

CONSTRUCTION OF A NEW HOST-VECTOR SYSTEM IN ARTHROBACTER SP AND CLONING OF THE LIPASE GENE
M MORIKAWA, H DAIDO, S PONGPOBPIBOOL, T IMANAKA
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 42, 2-3, 300, 303, Nov. 1994, [Peer-reviewed]
English, Scientific journal

CLONING OF THE AAPT GENE AND CHARACTERIZATION OF ITS PRODUCT, ALPHA-AMYLASE-PULLULANASE (AAPT), FROM THERMOPHILIC AND ALKALIPHILIC BACILLUS SP STRAIN XAL601
SP LEE, M MORIKAWA, M TAKAGI, T IMANAKA
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 60, 10, 3764, 3773, Oct. 1994, [Peer-reviewed]
English, Scientific journal

Cloning and analysis of 16S-rRNA gene and transcription factor (TF) IID gene from a hyperthermophilic archaeon strain KOD1.　　　　　　　　　　　　　　　
Rashid N, Morikawa M, Imanaka T
Annual reports of ICBiotech., 17, 255, 270, 1994, [Peer-reviewed]
English

超好熱始原菌 KOD1 株チオール型プロテアーゼ(TT プロテアーゼ) 遺伝子の検索　　　　　　　　　　　　　　　
森川 正章
アマシャムライフサイエンスHighlight, 12月, 1994, [Peer-reviewed]
Japanese

微生物による石油分解と石油生成
今中忠行, 森川正章
バイオサイエンスとインダストリー, 52, 11, 898, 900, バイオインダストリ-協会, 1994, [Peer-reviewed]
Japanese

CO2を単一炭素源として生育する石油代謝細菌　　　　　　　　　　　　　　　
森川正章, 今中忠行
化学と生物, 32, 690, 691, 1994, [Peer-reviewed]
Japanese

A NEW LIPOPEPTIDE BIOSURFACTANT PRODUCED BY ARTHROBACTER SP STRAIN MIS38
M MORIKAWA, H DAIDO, T TAKAO, S MURATA, Y SHIMONISHI, T IMANAKA
JOURNAL OF BACTERIOLOGY, 175, 20, 6459, 6466, Oct. 1993, [Peer-reviewed]
English, Scientific journal

真核生物のすぐ隣は超好熱菌
森川正章, 今中忠行
バイオサイエンスとインダストリー, 51, 5, 33, 37, バイオインダストリ-協会, 1993, [Peer-reviewed]
Japanese

石油を分解する細菌　　　　　　　　　　　　　　　
森川正章, 今中忠行
化学, 48, 692, 693, 1993, [Peer-reviewed]
Japanese

ISOLATION OF A NEW MIXOTROPHIC BACTERIUM WHICH CAN FIX CO2 AND ASSIMILATE ALIPHATIC AND AROMATIC-HYDROCARBONS ANAEROBICALLY
M MORIKAWA, T IMANAKA
JOURNAL OF FERMENTATION AND BIOENGINEERING, 76, 4, 280, 283, 1993, [Peer-reviewed]
English, Scientific journal

ISOLATION OF A NEW SURFACTIN PRODUCER BACILLUS-PUMILUS A-1, AND CLONING AND NUCLEOTIDE-SEQUENCE OF THE REGULATOR GENE, PSF-1
M MORIKAWA, M ITO, T IMANAKA
JOURNAL OF FERMENTATION AND BIOENGINEERING, 74, 5, 255, 261, 1992, [Peer-reviewed]
English, Scientific journal

細胞内タンパク質の選択的分解
森川正章, 今中忠行
バイオサイエンスとインダストリー, 49, 9, 969, 972, バイオインダストリ-協会, 1991, [Peer-reviewed]
Japanese

Cloning in Bacillus subtilis of thermostable and alkalophilic amylase from a thermophilic Bacillus sp.　　　　　　　　　　　　　　　
Ganrong X, Lee S-P, Takagi M, Morikawa M, Imanaka T
Annual reports of ICBiotech., 13, 121, 128, 1990, [Peer-reviewed]
English

A STRUCTURAL REQUIREMENT IN THE SUBSITE-F OF LYSOZYME - THE ROLE OF ARGININE-115 IN HUMAN LYSOZYME REVEALED BY SITE-DIRECTED MUTAGENESIS
M MURAKI, M MORIKAWA, Y JIGAMI, H TANAKA
EUROPEAN JOURNAL OF BIOCHEMISTRY, 179, 3, 573, 579, Feb. 1989, [Peer-reviewed]
English, Scientific journal

ENGINEERING OF HUMAN LYSOZYME AS A POLY-ELECTROLYTE BY THE ALTERATION OF MOLECULAR-SURFACE CHARGE
M MURAKI, M MORIKAWA, Y JIGAMI, H TANAKA
PROTEIN ENGINEERING, 2, 1, 49, 54, Apr. 1988, [Peer-reviewed]
English, Scientific journal

THE ROLES OF CONSERVED AROMATIC AMINO-ACID-RESIDUES IN THE ACTIVE-SITE OF HUMAN LYSOZYME - A SITE-SPECIFIC MUTAGENESIS STUDY
M MURAKI, M MORIKAWA, Y JIGAMI, H TANAKA
BIOCHIMICA ET BIOPHYSICA ACTA, 916, 1, 66, 75, Nov. 1987, [Peer-reviewed]
English, Scientific journal

ENGINEERING OF THE ACTIVE-SITE OF HUMAN LYSOZYME - CONVERSION OF ASPARTIC ACID-53 TO GLUTAMIC-ACID AND TYROSINE-63 TO TRYPTOPHAN OR PHENYLALANINE
M MURAKI, Y JIGAMI, M MORIKAWA, H TANAKA
BIOCHIMICA ET BIOPHYSICA ACTA, 911, 3, 376, 380, Feb. 1987, [Peer-reviewed]
English

Game Changing Environmental Purification Technology　　　　　　　　　　　　　　　
森川正章, Appl. Cell Biol. Japan, 34, 33, 54, Nov. 2021
Japanese

ウキクサホロビオントが開くグリーンサーキュラーエコノミーへの扉　　　　　　　　　　　　　　　
森川正章, バイオインダストリー, 38, 7, 2, 9, Jul. 2021, [Invited]
Japanese

植物・微生物機能の解析・制御と食糧・バイオマス生産への応用展開
浅見忠男, 安藤康雄, 森川正章, ほか, 日本土壌肥料学雑誌, 90, 1, 82, 87, 2019, [Invited]
Japanese, Report scientific journal, 10240737

ヒトの冬眠は環境バイオテクノロジー？　　　　　　　　　　　　　　　
森川正章, 環境バイオテクノロジー学会ニュースレター, 1, 1, 2018, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

グリーンケミストリーの基盤をささえる水生植物の成長を加速する微生物
森川正章, ケミカルエンジニヤリング, 61, 5, 368, 373, May 2016, [Invited], [Lead author, Corresponding author]
化学工業社, Japanese, Introduction commerce magazine, 10240737

汚水を用いたウキクサバイオマス生産とバイオリファイナリーへの応用
遠山忠, 森一博, 池道彦, 森川正章, バイオサイエンスとインダストリー, 74, 3, 203, 207, May 2016
バイオインダストリー協会, Japanese, Introduction other

紅藻マギレソゾLaurencia saitoi由来ブロモペルオキシダーゼの臭素化反応
正木志良, 金子賢介, 小林大毅, 石川高史, 西川慶祐, 森本善樹, 鷲尾健司, 森川正章, 沖野龍文, 日本農芸化学会大会講演要旨集(Web), 2016, 4B032 (WEB ONLY), 05 Mar. 2016
Japanese

紅藻ミツデソゾLaurencia okamurae由来ブロモペルオキシダーゼの性状解析
石川高史, 金子賢介, 湯暁蓉, 鷲尾健司, 森川正章, 沖野龍文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, ROMBUNNO.1B17, 2016
Japanese

紅藻ソゾ属3種のブロモペルオキシダーゼの活性評価
金子賢介, 小林大毅, 鷲尾健司, 森川正章, 沖野龍文, 日本化学会講演予稿集, 95th, 4, 1409, 11 Mar. 2015
Japanese

2P-169 Duckweed growth promotion by Sinorhizobium sp. SP4: transcriptome analysis of the effects of SP4 on duckweed growth
Toyama Tadashi, Tanaka Yasuhiro, Morikawa Masaaki, Mori Kazuhiro, 日本生物工学会大会講演要旨集, 67, 217, 217, 2015
日本生物工学会, Japanese

1P-167 Isolation and characterization of novel plant growth promoting bacteria (PGPB) from cormosphere of duckweed
Iwashita Tomoki, Tanaka Yasuhiro, Tamaki Hideyuki, Makino Ayaka, Toyama Tadashi, Kamagata Youichi, Morikawa Masaaki, Mori Kazuhiro, 日本生物工学会大会講演要旨集, 67, 130, 130, 2015
日本生物工学会, Japanese

次世代バイオマス：ウキクサの可能性
森川正章, 化学経済, 62, 14, 75, 79, 2015, [Invited], [Lead author, Corresponding author]
化学工業日報社, Japanese, Introduction commerce magazine, 10240737

廃水を肥料とするバイオマス生産技術の夜明け
森川正章, 遠山 忠, 田中靖浩, 森 一博, 玉木秀幸, 清 和成, 黒田真史, 三輪京子, 鎌形洋一, 池 道彦, 再生と利用, 39, 149, 50, 53, 2015, [Invited], [Lead author, Corresponding author]
日本下水道協会, Japanese, Introduction commerce magazine, 10240737

3P-149 Screening of Microalgae Growth-Promoting Bacteria
Quach Angela, Horino Taro, Kuroda Masashi, Toyama Tadashi, Tamaki Hideyuki, Morikawa Masaaki, Ike Michihiko, 日本生物工学会大会講演要旨集, 67, 308, 308, 2015
日本生物工学会, Japanese

紅藻ウラソゾLaurencia nipponica由来バナジウム依存型ブロモペルオキシダーゼの性状解析
KANEKO KENSUKE, WASHIO KENJI, UMEZAWA DAIKI, MATSUDA FUYUHIKO, MORIKAWA MASAAKI, OKINO TATSUFUMI, 日本水産学会大会講演要旨集, 2014, 119, 27 Mar. 2014
Japanese

P14-13 ウキクサ科植物の葉状体に生息する微生物群集の解析(ポスター発表)
田中 靖浩, 立野 由佳, 玉木 秀幸, 牧野 彩花, 遠山 忠, 鎌形 洋一, 森川 正章, 森 一博, 日本微生物生態学会講演要旨集, 2014, 153, 153, 2014
日本微生物生態学会, Japanese

P25-7 水生植物-微生物共生系を構成する新規植物成長促進根圏微生物(ポスター発表)
牧野 彩花, 玉木 秀幸, 遠山 忠, 田中 靖浩, 森 一博, 池 道彦, 森川 正章, 鎌形 洋一, 日本微生物生態学会講演要旨集, 2014, 242, 242, 2014
日本微生物生態学会, Japanese

3P-083 Isolation and characterization of phytobacteria that can highly adhere to duckweed
Yamakawa Yusuke, Sugawara Masayuki, Ozima Takuya, Miwa Kyoko, Morikawa Masaaki, 日本生物工学会大会講演要旨集, 66, 215, 215, 2014
日本生物工学会, Japanese

3P-082 Analyses of Microbial Communities Inhabiting Cormosphere and Rhizosphere of Lemnaceae
Tateno Yuka, Tanaka Yasuhiro, Tamaki Hideyuki, Makino Ayaka, Toyama Tadashi, Kamagata Yoichi, Morikawa Masaaki, Mori Kazuhiro, 日本生物工学会大会講演要旨集, 66, 215, 215, 2014
日本生物工学会, Japanese

3P-140 The Effects of General Plant Growth-Promoting Compounds on the Growth of duckweed Lemna minor :
Desi Utami, Masayuki Sugawara, Kyoko Miwa, Masaaki Morikawa, 日本生物工学会大会講演要旨集, 66, 229, 229, 2014
日本生物工学会, English

2P-137 Groeth promotion of land plant using Plant Growth-Promotion Rizobacteria Acinetobacer calcoaceticus P23
Kagemoto Keita, Sugawara Masayuki, Ojima Takuya, Miwa Kyoko, Morikawa Masaaki, Toyama Tadashi, 日本生物工学会大会講演要旨集, 66, 141, 141, 2014
日本生物工学会, Japanese

1P-060 Purification and characterization of novel azo-reductases from Flavobacteriaceae Elizabethkingia sp. RL13212
Yasukata Kuniki, Murakami Shun, Miwa Kyoko, Morikawa Masaaki, 日本生物工学会大会講演要旨集, 66, 32, 32, 2014
日本生物工学会, Japanese

新規高度好熱菌Coprothermobacter sp.PM9-2とメタン生成菌の共生に関わる分子機構の解析
加藤雄大, 漆畑亘, 三輪京子, 森川正章, 日本生物工学会大会講演要旨集, 66th, 2014

New Function of the Phytosphere Bacteria from Aquatic Plants
Masaaki MORIKAWA, Masayuki SUGAWARA, Wakako SUZUKI, Kyoko MIWA, 化学と生物, 52, 12, 799, 804, 2014, [Invited], [Lead author, Corresponding author]
Japanese, Introduction other

紅藻ウラソゾLaurencia nipponica由来バナジウム依存型ブロモペルオキシダーゼの性状解析
KANEKO KENSUKE, WASHIO KENJI, UMEZAWA DAIKI, MATSUDA FUYUHIKO, MORIKAWA MASAAKI, OKINO TATSUFUMI, マリンバイオテクノロジー学会大会講演要旨集, 15th, 68, 01 Jun. 2013
Japanese

ウキクサ科植物の埋立地浸出水処理への適用性に関する実験的検討
尾形有香, 石垣智基, 山田正人, 遠山忠, 清和成, 森川正章, 池道彦, 全国都市清掃研究・事例発表会講演論文集, 35th, 2013

1P-163 Exploration and characterization of duckweed rhizobacteria that produce useful compounds
Mori Shohei, Kurashina Koki, Sugawara Masayuki, Miwa Kyoko, Morikawa Masaaki, 日本生物工学会大会講演要旨集, 65, 58, 58, 2013
日本生物工学会, Japanese

1P-200 Sinorhizobium sp. SP4 enhances photosynthesis, biomass production and nutrient uptake of duckweed
Toyama Tadashi, Tanaka Yasuhiro, Mori Kazuhiro, Morikawa Masaaki, 日本生物工学会大会講演要旨集, 65, 67, 67, 2013
日本生物工学会, Japanese

3P-224 Possibility of duckweed rhizobacterium Acinetobacter sp. P23 for the growth promotion of vegetables
Suzuki Wakako, Sugawara Masayuki, Miwa Kyoko, Morikawa Masaaki, 日本生物工学会大会講演要旨集, 65, 244, 244, 2013
日本生物工学会, Japanese

海底油田から単離した高度好熱菌Coprothermobacter sp.PM9-2株の諸特性解析
加藤雄大, 漆畑亘, 三輪京子, 森川正章, 森川正章, 環境バイオテクノロジー学会大会プログラム講演要旨集, 2013, 2013

3P-149 Sustainable growth promotion of Lemna minor by Acinetobacter calcoaceticus P23 in environmental water
Hachiya Yoshiyuki, Quach Angela, Tokura Koichiro, Ogata Yuka, Kuroda Masashi, Toyama Tadashi, Morikawa Masaaki, Ike Michihiko, 日本生物工学会大会講演要旨集, 65, 225, 225, 2013
日本生物工学会, Japanese

Unique microbiological characteristics of Pseudoalteromonas spp. that form biofilms in the sea water environment : Their possible contribution to water purification and fish farming.　　　　　　　　　　　　　　　
Iijima S, Okahara R, Washio K, Morikawa M, Bulletin of the Society of Sea Water Science, 66, 186, 190, 2012, [Peer-reviewed]
Japanese, Introduction scientific journal

4Gp10 Colonization of Acinetobacter calcoaceticus strain P23 on rhizosphere of duckweed, Lemna minor
HACHIYA Yoshiyuki, QUACH Angela, OGATA Yuka, KURODA Masashi, INOUE Daisuke, MORIKAWA Masaaki, IKE Michihiko, 日本生物工学会大会講演要旨集, 64, 225, 225, 2012
日本生物工学会, Japanese

1Hp04 Mixed biofilm formation by Marinobacter bacteria and relatives
Morikawa Masaaki, Eiki Hisashi, Tarakita Natsumi, Washio Kenji, 日本生物工学会大会講演要旨集, 63, 54, 54, 2011
日本生物工学会, Japanese

The role of urease activity on biofilm and urolith formation by Staphylococcus sp T-02 that was isolated from a toilet bowl
K. Oki, K. Washio, D. Matsui, Y. Hirata, M. Morikawa, JOURNAL OF BIOTECHNOLOGY, 150, S247, S247, Nov. 2010
English, Summary international conference

Pseudomonas stutzeriバイオフィルムのナフタレン分解活性と土壌安定性
嶋田恒平, 片岡剛文, 鷲尾健司, 森川正章, 日本農芸化学会大会講演要旨集, 2008, 39, 05 Mar. 2008
Japanese

S-C5 Sustainable bioremediation technology utilizing Biofilms
MORIKAWA Masaaki, WASHIO Kenji, 日本微生物生態学会講演要旨集, 24, 193, 193, 2008
日本微生物生態学会, Japanese

Pseudomonas sutzeriバイオフィルムのナフタレン分解活性と土壌安定性
嶋田恒平, 片岡剛文, 鷲尾健司, 森川正章, 環境バイオテクノロジー学会大会プログラム講演要旨集, 31st, 14, 2007
Japanese

Expression of rice germination-control genes that accompany with the change of DNA modification.
K Washio, M Morikawa, PLANT AND CELL PHYSIOLOGY, 47, S147, S147, 2006
English, Summary international conference

1G17-3 Biofilm formation and protease production by Pseudoalteromonas sp. SB-B1
IIJIMA Saori, OKAHARA Ryota, TAKANO Kazufumi, KANAYA Shigenori, MORIKAWA Masaaki, 日本生物工学会大会講演要旨集, 17, 196, 196, 2005
日本生物工学会, Japanese

1A16-3 Analyses on the alkane degrading genes of Rhodococcus sp. TMP2
TAKEI Daisuke, KUNIHIRO Namio, TAKANO Kazufumi, KANAYA Shigenori, MORIKAWA Masaaki, 日本生物工学会大会講演要旨集, 17, 56, 56, 2005
日本生物工学会, Japanese

3S4-AM4 Studies on the biofilm formation by a wild-type Bacillus subtilis
MORIKAWA Masaaki, KANAYA Shigenori, IMANAKA Tadayuki, 日本生物工学会大会講演要旨集, 17, 47, 47, 2005
日本生物工学会, Japanese

Dissection of DNA methylation status in gibberellin-primary response genes that involve the germination of rice seeds.
K Washio, M Morikawa, PLANT AND CELL PHYSIOLOGY, 46, S130, S130, 2005
English, Summary international conference

3P020 Determination of the conformation and configuration of arthrofactin by NMR
Kondo Y, Morikawa M, Ikegami T, Seibutsu Butsuri, 45, 0, S208, 2005
一般社団法人 日本生物物理学会, Japanese

2E11-3 Biofilm bacteria isolated from a fisheries nursery
OKAHARA Ryota, ISHIABSHI Kanehisa, OKADA Chihiro, TAKANO Kazufumi, MORIKAWA Masaaki, IMANAKA Tadayuki, KANAYA Shigenori, 日本生物工学会大会講演要旨集, 16, 168, 168, 2004
日本生物工学会, Japanese

2D14-2 Isolation and characterization of microorganisms that degrade plastic elasticizer (DEHP)
MORIKAWA Masaaki, CHIN Shimei, TAKANO Kazufumi, IMANAKA Tadayuki, KANAYA Shigenori, 日本生物工学会大会講演要旨集, 16, 156, 156, 2004
日本生物工学会, Japanese

リボヌクレアーゼの多様性と酵素機能 Ribonuclease H RNA界とDNA界の間のRibonuclease(Molecular diversities and functions of ribonucleases: RNase H: a ribonuclease between RNA and DNA worlds)　　　　　　　　　　　　　　　
Tsunaka Yasuo, Chon Hyongi, Takano Kazufumi, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 生化学, 75, 8, 728, 728, Aug. 2003
(公社)日本生化学会, English

Analysis of a pro-sequence of subtilisin from hyperthermophilic archaeon
Saito Kenji, Tanaka Shihori, Takano Kazuhumi, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 15, 60, 60, 2003
日本生物工学会, Japanese

2B16-2 Substrate specificity of the internal adenylation domains in arthrofactin synthetase :
Roongsawang Niran, Takatsuka Atsuko, Takano Kazufumi, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 15, 76, 76, 2003
日本生物工学会, English

Biofilm formation and environmental adaptation
Morikawa Masaaki, Kajihiro Shinji, Haruki Mitsuru, Takano Kazufumi, Imanaka Tadayuki, Kolter Roberto, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 15, 42, 42, 2003
日本生物工学会, Japanese

Analysis of the genes required for the biofilm formation in Bacillus subtilis
Kawai Yoshitaka, Takano Kazufumi, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 15, 145, 145, 2003
日本生物工学会, Japanese

Studies on gentisate 1,2-dioxygenase from polyaromatic hydrocarbon degrading bacterium strain 127W
Hirano Shinichi, Takano Kazufumi, Imanaka Tadayuki, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 15, 218, 218, 2003
日本生物工学会, Japanese

501 Stability analysis of protein from hyperthermophilic archaeon
Mukaiyama Atsushi, Chon Hyongi, Takano Kazufumi, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 73, 73, 2002
日本生物工学会, Japanese

476 Complete sequence of arthrofactin biosynthesis operon with unique two carboxy-terminal thioesterase domains :
Roongsawang Niran, Hase Kenichi, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 69, 69, 2002
日本生物工学会, English

505 FKBP from Psychrotrophic Bacterium Shewanella sp. SIB1
Suzuki Yutaka, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 74, 74, 2002
日本生物工学会, Japanese

920 Cloning and analysis of PAHs degrading genes from newly isolated bacterium strain 127W
Hirano Shin-ichi, Haruki Mitsuru, Imanaka Tadayuki, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 169, 169, 2002
日本生物工学会, Japanese

671 Analysis for the function of a C-terminal domain of a family I.3 lipase and its application
Kuwahara Katsumasa, Kwon Hyun Ju, Haruki Mitsuru, Morikawa Masaaki, Oomori Kenji, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 112, 112, 2002
日本生物工学会, Japanese

681 Cleavage specificities of prokaryotic type 2 ribonucleases H on DNA-RNA-DNA/DNA chimeric substrates
Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 115, 115, 2002
日本生物工学会, Japanese

679 Role of a putative pro-sequence of subtilisin from a hyperthermophilic archaeon
Tanaka Shihori, Saito Kenji, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 114, 114, 2002
日本生物工学会, Japanese

645 Psychrotrophic Type 2 RNase H with unusual enzymatic characteristics
Chon Hyongi, Ohtani Naoto, Haruki Mituru, Takano Kazufumi, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 106, 106, 2002
日本生物工学会, Japanese

408 Identification and characterization of an oil-degrading bacterium strain HD-1
Kanamori Takeshi, Rashid Naeem, Morikawa Masaaki, Atomi Haruyuki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 14, 52, 52, 2002
日本生物工学会, Japanese

871 Genetic analysis on biofilm formation by Bacillus subtilis B-1
Kawai Yoshitaka, Kameyama Takayuki, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 160, 160, 2002
日本生物工学会, Japanese

903 Isolation and characterization of two types of psychrotrophic Rhodococcus strains degrading 2,6,10,14-tetramethylpentadecane (pristane)
Kunihiro Namio, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 165, 165, 2002
日本生物工学会, Japanese

913 Gene expression and characterization of alcohol dehydrogenase from Bacillus thermoleovorans B23
Nishikawa Hiroshi, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 168, 168, 2002
日本生物工学会, Japanese

680 Studies on the catalytic mechanism of Escherichia coli RNase HI in the presence of Mn^<2+>
Tsunaka Yasuo, Haruki Mitsuru, Oobatake Motohisa, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 14, 114, 114, 2002
日本生物工学会, Japanese

耐熱性ハイブリッドRNase HIによるRNAの配列特異的分解
全賢基, 津中康央, 春木満, 森川正章, 金谷茂則, 生化学, 73, 8, 960, 25 Aug. 2001
Japanese

Interaction of TIP26 from a hyperthermophilic archaeon with TFB/TBP/DNA ternary complex (vol 5, pg 177, 2001)
T Matsuda, M Fujikawa, M Haruki, XF Tang, S Ezaki, T Imanaka, M Morikawa, S Kanaya, EXTREMOPHILES, 5, 4, 283, 283, Aug. 2001
English, Others

Identification of the histidine and aspartic acid residues essential for enzymatic activity of a family I.3 lipase by site-directed mutagenesis (vol 483, pg 139, 2000)
HJ Kwon, K Amada, M Haruki, M Morikawa, S Kanaya, FEBS LETTERS, 497, 2-3, 174, 174, May 2001
English, Others

Development of an RNA restriction enzyme by using ribonuclease H
Kanaya Shigenori, Morikawa Masaaki, Haruki Mitsuru, Nogawa Tomoko, Tsunaka Yasuhiro, Chon Hyongi, 日本生物工学会大会講演要旨集, 13, 12, 12, 2001
日本生物工学会, Japanese

大腸菌リボヌクレアーゼHIの触媒作用における基質のリン酸基の寄与
春木満, 津中康央, 森川正章, 金谷茂則, 岩井成憲, 生化学, 72, 8, 866, 25 Aug. 2000
Japanese

Gene cloning and characterization of a novel alcohol dehydrogenase from Bacillus thermoleovorans B23.
Kato Tomohisa, Miyanaga Asuka, Haruki Mitsuru, Morikawa Masaaki, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 12, 70, 70, 2000
日本生物工学会, Japanese

Degradation of aromatic hydrocarbons under microaerobic conditions
Hirano Shin-ichi, Kitauchi Fumiya, Haruki Mitsuru, Imanaka Tadayuki, Morikawa Masaaki, Kanaya Sigenori, 日本生物工学会大会講演要旨集, 12, 122, 122, 2000
日本生物工学会, Japanese

Exploring of the characteristic metabolism in oil-degrading/producing bacterium strain HD-1 by random sequencing
Fujikawa Makoto, Haruki Mitsuru, Imanaka Tadayuki, Morikawa Masaaki, Kanaya Sigenori, 日本生物工学会大会講演要旨集, 12, 132, 132, 2000
日本生物工学会, Japanese

Analysis of alkane induced proteins and an aldehyde dehydrogenase from Bacillus thermoleovorans B23.
Kato Tomohisa, Miyanaga Asuka, Haruki Mitsuru, Morikawa Masaaki, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 12, 118, 118, 2000
日本生物工学会, Japanese

二価金属イオンを要求しない大腸菌RNase HI変異体の解析
津中康央, 春木満, 森川正章, 金谷茂則, 日本分子生物学会年会プログラム・講演要旨集, 22nd, 507, 22 Nov. 1999
Japanese

Analysis of anaerobic alkane degradation pathway by 2D-PAGE
Komatsu Ryuta, Matsuda Tomoki, Haruki Mitsuru, Imanaka Tadayuki, Morikawa Masaaki, Kaaya Shigenori, 日本生物工学会大会講演要旨集, 11, 49, 49, 1999
日本生物工学会, Japanese

Studies on calcium dependent lipase from Pseudomonad.
Amada Kei, Haruki Mitsuru, Morikawa Masaaki, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 11, 76, 76, 1999
日本生物工学会, Japanese

Cloning of Arthrofactin synthetic genes.
Hase Kenichi, Amada Kei, Nagahisa Keisuke, Haruki Mitsuru, Imanaka Tadayuki, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 11, 140, 140, 1999
日本生物工学会, Japanese

Gene cloning and structure analysis of enzymes from a psychrotrophic bacterium Shewanella sp. SIB1
Miura Keiko, Ohtani Naoto, Sato Akihiro, Kato Tomohisa, Tomoe Yasuyoshi, Haruki Mitsuru, Morikawa Masaaki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 11, 141, 141, 1999
日本生物工学会, Japanese

Characterization of Bacillus thermoleovorans B23, H41.
Kato Tomohisa, Morikawa Masaaki, Haruku Mitsuru, Tomoe Yasuyoshi, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 11, 298, 298, 1999
日本生物工学会, Japanese

Degadation of n-alkanes by Bacillus thermoleovorans B23. H41.
Kato Tomohisa, Miyanaga Asuka, Morikawa Masaaki, Haruki Mitsuru, Tomoe Yasuyoshi, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 11, 299, 299, 1999
日本生物工学会, Japanese

Petroleum production from CO2
M Morikawa, S Jin, S Kanaya, T Imanaka, NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 72, 4, 532, 534, Apr. 1998
Japanese, Book review

Characterization of subtilisin homologue from a hyperthermophilic archaeon
Inoue Y., Morikawa M., Haruki M., Takagi M., Imanaka T., Kanaya S., 日本生物工学会大会講演要旨集, 10, 280, 280, 1998
日本生物工学会, Japanese

1156 Characterization of petroleum degrading bacteria from oil contaminated sea and land in Vietnam :
Huy Nguyen Quang, Jin Sha, Amada Kei, Morikawa Masaaki, Haruki Mitsuru, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 10, 295, 295, 1998
日本生物工学会, English

Degradation of Crude Oil under Anoxic Conditions by a Newly Isolated Bacterium
Jin Sha, Morikawa Masaaki, Haruki Mitsuru, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 10, 294, 294, 1998
日本生物工学会, Japanese

Isolation and characterization of novel bacteria from oil fields.
Kato Tomohisa, Funayama Tetsu, Morikawa Masaaki, Haruki Mitsuru, Tomoe Yasuyoshi, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 10, 294, 294, 1998
日本生物工学会, Japanese

Characterization of a newly isolated thermophilic bacterium from an oil field under the bottom sea.
Funayama Tetsu, Morikawa Masaaki, Haruki Mitsuru, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 10, 295, 295, 1998
日本生物工学会, Japanese

Gene cloning and characterization of aldehyde dehydrogenase from petroleum dissimilating/assimilating bacterium HD-1
Okibe Naoko, Morikawa Masaaki, Amada Kei, Haruki Mitsuru, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 10, 123, 123, 1998
日本生物工学会, Japanese

Gene cloning and characterization of lipase from biosurfactant producing strain Arthrobacter sp. MIS38.
Amada Kei, Morikawa Masaaki, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 9, 12, 12, 1997
日本生物工学会, Japanese

Development of a culture system for the CO_2 fixation and alkane production by microorganisms.
Jin Sha, Morikawa Masaaki, Funayama Tetsu, Haruki Mitsuru, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 9, 246, 246, 1997
日本生物工学会, Japanese

Preparation of transcriptional initiation complex by using hyperthermophilic archaeal TATA binding protein as affinity ligand.
Matsuda Tomoki, Morikawa Masaaki, Haruki Mitsuru, Imanaka Tadayuki, Kanaya Shigenoli, 日本生物工学会大会講演要旨集, 9, 158, 158, 1997
日本生物工学会, Japanese

Cloning of aldehyde dehydrogenase gene from photosynthetic bacterium Rhodospirillum rubrum
Okibe Naoko, Morikawa Masaaki, Amada Kei, Haruki Mitsuru, Imanaka Tadayuki, Kanaya Shigenori, 日本生物工学会大会講演要旨集, 9, 12, 12, 1997
日本生物工学会, Japanese

超好熱始原菌Pyrococcus sp. KOD1 由来 Glycerol Kinase のクローニングと解析
古賀 雄一, 森川 正章, 春木 満, 今中 忠行, 金谷 茂則, 日本分子生物学会年会プログラム・講演要旨集, 19, 136, 136, Aug. 1996
Japanese

Thermus thermophilus RNase H C末端欠損変異体の熱安定性解析
平野 展孝, 春木 満, 森川 正章, 大畠 玄久, 金谷 茂則, 日本分子生物学会年会プログラム・講演要旨集, 19, 716, 716, 01 Aug. 1996
Japanese

微生物による石油分解と石油生成 (特集/バイオテクノロジ-最近の話題)
森川 正章, 今中 忠行, 化学工業, 47, 5, 365, 371, May 1996
化学工業社, Japanese

Isolation of an esterase producing bacterium and cloning of the gene.
Jinno Kazuhito, Morikawa Masaaki, Matsumoto Shusaku, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 8, 186, 186, 1996
日本生物工学会, Japanese

454 RecA homologue from a hyperthermophilic archaeon maintains the major domain only :
Rashid Naeem, Morikawa Masaaki, Kanaya Shigenori, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 8, 123, 123, 1996
日本生物工学会, English

Surface-chemical characterization of arthrofactin.
Morikawa Masaaki, Hirata Yoshihiko, Horiuchi Teruo, Ohdera Motoyasu, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 8, 290, 290, 1996
日本生物工学会, Japanese

Isolation of dibenzothiophene degradation bacteria from petroleum.
Kitauchi Fumiya, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 8, 253, 253, 1996
日本生物工学会, Japanese

Characterization and improvemont of petroleum producing bacterium strain HD-1.
Morikawa Masaaki, Ikeda Masaru, Nagahisa Keisuke, Haruki Mitsuru, Kanaya Shigenori, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 8, 252, 252, 1996
日本生物工学会, Japanese

細菌によるCO_2固定と石油生産(2) : 環境科学
森川 正章, 岩佐 毅, 清水 一朗, 永久 圭介, 柳田 祥三, 今中 忠行, 日本農藝化學會誌, 69, 377, 377, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

超好熱始原菌KOD1株由来DNAポリメラーゼの特性 : 酵素
北林 雅夫, 荒川 琢, 井上 浩明, 川上 文清, 川村 良久, 高木 昌宏, 森川 正章, 今中 忠行, 日本農藝化學會誌, 69, 246, 246, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

Petroleum production by microorganisms
M. Morikawa, T. Imanaka, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 40, 1, 52, 60, 1995
Japanese, Book review

Studies on the structure-activity relationship of biosurfactants.
Hirata Yoshihiko, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 7, 264, 264, 1995
日本生物工学会, Japanese

Genome analysis of 16S rRNA flanking region of Pyrococcus sp. KOD1.
Morikawa Masaaki, Fujiwara Shinsuke, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 7, 215, 215, 1995
日本生物工学会, Japanese

Studies on the membrane domain of signal sensor protein ComP.
Pongpobpibool Sutee, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 6, 221, 221, 1994
日本生物工学会, Japanese

Studies on the structure-activity relationship of biosurfactant.
Hirata Yoshihiko, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 6, 214, 214, 1994
日本生物工学会, Japanese

656 Cloning and Analysis of 16 S-rRNA gene and Transcriptional Factor TF IID gene from Hyperthermophilic Archaeum strain KOD 1 :
Rashid Naeem, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 6, 236, 236, 1994
日本生物工学会, English

Characterization of amylase pullulanase from thermophilic and alkaliphilic Bacillus sp.
Lee Sang Pil, Morikawa Masaaki, Takagi Masahiro, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 6, 87, 87, 1994
日本生物工学会, Japanese

Cloning and analsis of the DNA polymerase gene from a new hyperthermophilc archaeon KOD1
Kakihara Hirofumi, Takagi Masahiro, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 5, 168, 168, 1993
日本生物工学会, Japanese

Degradation of saturated aliphatic hydrocarbon by P__-. anaerooleophila HD-1
Kanemoto Mitsuhide, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 5, 144, 144, 1993
日本生物工学会, Japanese

Isolation and Application of Thermophilic Bacteria
Imanaka Tadayuki, Takagi Masahiro, Morikawa Masaaki, 日本生物工学会大会講演要旨集, 5, 10, 10, 1993
日本生物工学会, Japanese

Purification and characterization of a thermostable thiol protease from Pyrococcus sp. KOD1
伊澤 啓文, 八木 正貴, 森川 正章, 今中 忠行, 帆秋 利洋, 日本生物工学会大会講演要旨集, 5, 224, 224, 1993
日本生物工学会, Japanese

Cloning and expression of anti-porphyrin monoclonal antibody gene in Escherichia coil.
Ueda Makoto, Hamuro Takuya, Morikawa Masaaki, Harada Akira, Kamati Mikiharu, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 4, 30, 30, 1992
日本生物工学会, Japanese

Cloning and sequencing of the gene for surfactin production.
Morikawa Masaaki, Suzuki Masanori, Goto Shinya, Ito Mikito, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 4, 34, 34, 1992
日本生物工学会, Japanese

Isolation of a new biosurfactant producer and structure analysis of its product.
Daido Hiromi, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 4, 217, 217, 1992
日本生物工学会, Japanese

Examination of culture condition for P__-. anaerooleophila HD-1.
Morikawa Masaaki, Sato Yoshiaki, Imanaka Tdayuki, 日本生物工学会大会講演要旨集, 4, 165, 165, 1992
日本生物工学会, Japanese

Cloning of PPlase gene from Bacillus stearothermophilus SIC1
Kim Dongju, Morikawa Masaaki, Imanaka Tadayuki, 日本生物工学会大会講演要旨集, 4, 125, 125, 1992
日本生物工学会, Japanese

Isolation and characterization of hyper-thermophilic archaebacteria from hot spring.
Izawa Yoshifumi, Kakihara Hirofumi, Morikawa Masaaki, Imanaka Tadayuki, Hoaki Toshihiro, 日本生物工学会大会講演要旨集, 4, 207, 207, 1992
日本生物工学会, Japanese

Electron microscopic observation of Pseudomonas anaerooilphilus HD-1
Morikawa Masaaki, Imanaka Tadayuki, 日本醗酵工学会大会講演要旨集, 3, 114, 114, 1991
日本醗酵工学会, Japanese

Isolation of a facultative anaerobe which can grow anaerobicaly on n-tetradecane as a sole carbon source.
Morikawa Masaaki, Imanaka Tadayuki, 日本醗酵工学会大会講演要旨集, 2, 166, 166, 1990
日本醗酵工学会, Japanese

Low Cost Water and Wastewater Treatment Systems: Conventional and Recent Advances　　　　　　　　　　　　　　　
Oct. 2024, 9780443236624, [Joint work]

Remediation by Floating Plants
Masaaki Morikawa
Jan. 2023, 33532531, [Single work]

Bioremediation: From Key Enzymes to Practical Technologies
Masaaki Morikawa
Springer, Jan. 2023, 33532531, [Single work]

Design of Materials and Technologies for Environmental Remediation
Shunitz Tanaka, Masaaki Kurasaki, Masaaki Morikwa, Yuichi Kamiya
Springer, Jan. 2023, 29262199, [Joint editor]

バイオサーファクタント”、第10章低炭素社会への取組み、「応用微生物学 第３版」　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
文永堂出版, 2016

Chap. 12. Comparison of the degradation activity of biofilm-associated versus planktonic cells pp219-232　　　　　　　　　　　　　　　
Morikawa M, Washio K
Caister Academic Press, 2016

Chap. 13A Using microbial biofilms to enhance the phytoremediation of contaminants in soil and water – A trial for sustainable phenol degradation by duckweed-colonizing biofilms pp 233-240　　　　　　　　　　　　　　　
Morikawa M, Yamaga F, Suzuki K, Kurashina K, Miwa K, Washio K
Caister Academic Press, 2016

植物機能のポテンシャルを活かした環境保全・浄化技術　　　　　　　　　　　　　　　
森川正章, 第４章３節 PGPR (Plant Growth Promoting Rhizobacteria) の環境保全・修復への利用 pp 169-176
シーエムシー出版, 2011, [Contributor]

微生物増殖学の現在・未来　　　　　　　　　　　　　　　
森川 正章, バイオフィルム―微生物の生存戦略を生かすpp 444-454
地人書館, 2009, [Contributor]

バイオフィルムの基礎と制御　　　　　　　　　　　　　　　
森川正章, 鷲尾健司, 将来展望 バイオフィルムがもたらすブレークスルーの可能性pp 389-399, バイオサーファクタントを利用したバイオフィルムの形成と阻害pp 278-287
エヌティーエス, 2008, [Contributor]

ナノバイオ大事典　　　　　　　　　　　　　　　
森川 正章, バイオフィルム pp 420-423
テクノシステム, 2007, [Contributor]

バイオプロセスハンドブック　　　　　　　　　　　　　　　
森川 正章, 4章 微生物工学の基礎 pp 111-122
エヌティーエス, 2007, [Contributor]

環境修復の科学と技術　　　　　　　　　　　　　　　
森川 正章, 第４章３節 バイオレメディエーション pp150-186
北海道大学出版会, 2007, [Contributor]

生物学（上）　　　　　　　　　　　　　　　
森川 正章, １４章
培風館, 2006, [Joint translation]

食品のストレス環境と微生物　　　　　　　　　　　　　　　
森川 正章, 第9章1節．バイオフィルムの生成と生存メカニズム pp 221-228
サイエンスフォーラム, 2004, [Contributor]

農芸化学の事典　　　　　　　　　　　　　　　
森川 正章, 6.3.2. 直鎖状炭化水素の分解 pp749-751
朝倉書店, 2003, [Contributor]

微生物利用の大展開　　　　　　　　　　　　　　　
森川正章, ロベルト・コルター, 4.2.3. バイオサーファクタント pp 993-1002, 1.3.2. バイオフィルム–微生物たちの街 pp 178-181
エヌティーエス, 2002, [Contributor]

生命工学　　　　　　　　　　　　　　　
森川 正章, 第６章 微生物工学 pp155-179
共立出版, 2000, [Contributor]

生物工学実験書　　　　　　　　　　　　　　　
森川正章, 高木昌宏, 今中忠行, 第4章(4.4.2) 第5章(5.4.2)
培風館, 1992, [Contributor]

Enhanced Duckweed Biomass Production Utilizing Novel Plant Growth-Promoting Bacteria　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
China-Japan Environmental Microbiology Forum 2016, Chongqing, China, 13 Nov. 2016
[Invited]

有用微生物の農作物への新しい展開とその将来像　　　　　　　　　　　　　　　
森川 正章
日本生物工学会2016年度大会シンポジウム 於、富山国際会議場, 29 Sep. 2016
[Invited]

Insight into aquatic plant-microbe interaction　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Thai Research Expo, 2016, Bangkok, Thai, 18 Aug. 2016
[Invited]

海底メタン生産環境中の共生関係機構の解析　　　　　　　　　　　　　　　
森川 正章
日本農芸化学会2016年度大会シンポジウム 於、札幌コンベンションセンター, 28 Mar. 2016
[Invited]

微生物伝播の足場“バイオフィルム”　　　　　　　　　　　　　　　
森川 正章
日本細菌学会第89回総会シンポジウム 於、大阪国際交流センター, 23 Mar. 2016
[Invited]

Mutualistic biofilms- benefits for plants and animals　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
The 27th Annual Meeting of the Thai Society for Biotechnology and international conference (TSB2015) Bangkok, Thailand, 17 Nov. 2015
[Invited]

A new plant breeding technology enforcing mutualistic interaction between aquatic plants and their associated bacteria　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
International Symposium on Microbial Research and Biotechnology for Biomass Utilization. JR HAKATA CITY, Fukuoka, Japan, 12 Nov. 2015
[Invited]

根圏微生物共生系を活用した高次植生バイオプロセスの開発　　　　　　　　　　　　　　　
森川 正章
2015年度北日本支部仙台シンポジウム 於、仙台市, 04 Sep. 2015
[Invited]

水生植物と表層微生物の新しい共生メカニズム　　　　　　　　　　　　　　　
森川 正章
日本農芸化学会2015年度大会シンポジウム 於、岡山大学, 29 Mar. 2015
[Invited]

Plant growth-promoting bacteria are natural and powerful tools for enhancement of duckweed production　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
– Construction of an international research network for duckweed and scheme for the application to water environmental improvement –SPIRITS program Kyoto University Dept. Botany, Grad. Sch. Sci., Kyoto Univ., 05 Mar. 2015
[Invited]

微生物に魅せられて　　　　　　　　　　　　　　　
森川 正章
日清オイリオグループ株式会社 研究所内講演会 於、横須賀市, 18 Dec. 2014
[Invited]

水生植物の高機能化による革新的植生浄化技術の開発　　　　　　　　　　　　　　　
森川 正章
日本水処理生物学会第51回大会 於、甲府市, 12 Nov. 2014
[Invited]

Isolation and characterization of biosurfactant producing microorganisms from natural resources in Thailand　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
New Core to Core Program Advanced Research Networks. Bangkok, Thailand, 10 Aug. 2014
[Invited]

Development of effective bioremediation technology utilizing beneficial biofilms　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
New Core to Core Program Advanced Research Networks. Brawijaya Univesity, Indonesia, 08 Aug. 2014
[Invited]

好熱菌の諸特性〜その特異な酵素から生態まで　　　　　　　　　　　　　　　
森川 正章
2014年度第一回バイオ単分子研究会 於、伊豆市, 26 Jul. 2014
[Invited]

Application of Beneficial Plant-Microbe Interaction for Sustainable Industry　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Joint Symposium on Environmental Science 2013 –Bridging Finland and Japan – at University of Helsinki, Helsinki, Finland, 27 Nov. 2013
[Invited]

バイオフィルム工学による効率的な環境汚染物質分解　　　　　　　　　　　　　　　
森川 正章
JSR株式会社社内講演会（四日市市）, 06 Sep. 2013
[Invited]

水生植物の根圏強化による省エネ型水質浄化　　　　　　　　　　　　　　　
森川 正章
2013年度 環境バイオテクノロジー学会シンポジウム 於、北九州国際会議場（北九州市）, 01 Jun. 2013
[Invited]

High Temperature Petroleum Reservoir Microbiology　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Plenary Lecture. 42nd Convention Philippine Society for Microbiology, Tagaytay, Philippine, 18 Apr. 2013
[Invited]

教育講演 細菌の生存戦略としてのバイオフィルム形成　　　　　　　　　　　　　　　
森川 正章
47回緑膿菌感染症研究会, 23 Feb. 2013
[Invited]

Upgrading the function of aquaplants utilizing non-legume rhizobacteria　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
International Workshop for Plant Response to Mineral Stress, 07 Dec. 2012, English, Invited oral presentation
[Invited], [International presentation]

Beneficial biofilm formation by environmental bacteria in oil contaminated soils and the rhizosphere of duckweeds　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Microbiology Seminars at University of Helsinki, 25 Oct. 2012, English, Invited oral presentation
[Invited], [International presentation]

Could the utilization of biofilms, surface attached microbial communities, lead to a game-change in green biotechnology?　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Lecture of Environmental Biotechnology at Aalto University, 25 Oct. 2012, English, Invited oral presentation
[Invited], [International presentation]

Could the utilization of biofilms, surface attached microbial communities, lead to a game-change in green biotechnology?　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Royal Swedish Academy of Engineering Sciences (IVA) Lecture, 24 Oct. 2012, English, Invited oral presentation
[Invited], [International presentation]

Acinetobacter calcoaceticus P23のコウキクサの根圏への付着に関する検討　　　　　　　　　　　　　　　
蜂谷祥之, QUACH Angela, 尾形有香, 黒田真史, 井上大介, 森川正章, 池道彦
平成２４年度生物工学会, 24 Oct. 2012, Japanese, Oral presentation
[Domestic Conference]

Beneficial biofilm formation by environmental bacteria in oil contaminated soils and the rhizosphere of duckweeds　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Seminar at Uppsala Biocenter SLU, 23 Oct. 2012, English, Invited oral presentation
[Invited], [International presentation]

Could the utilization of biofilms, surface attached microbial communities, lead to a game-change in green biotechnology?　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Lecture at Technical University of Denmark, 21 Oct. 2012, English, Invited oral presentation
[Invited], [International presentation]

Cyclic lipopeptide antibiotics as Hsp90 inhibitors　　　　　　　　　　　　　　　
H. Nakamoto, Y. Yokoyama, S. Minagawa, Y. Kondoh, K. Sueoka, H. Osada, M. Morikawa
6th International Conference on the Hsp90 Chaperone Machine, 19 Sep. 2012, English, Poster presentation
[International presentation]

低温菌Shewanella sp. SIB1由来エステラーゼの取得および特性解析　　　　　　　　　　　　　　　
小林隆一, 平野展孝, 森川正章, 金谷茂則, 春木 満
平成２４年度化学系学協会東北大会, 15 Sep. 2012, Japanese, Poster presentation
[Domestic Conference]

How do bacteria treat with interfaces and surfaces?　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Invited seminar at L'Oréal Aulnay Research Center, 14 Sep. 2012, English, Invited oral presentation
[Invited], [International presentation]

Characterization of an extremely thermophilic bacterium, Coprothermobacter sp. PM9-2, isolated from an undersea petroleum reservoir　　　　　　　　　　　　　　　
W. Urushibata, T. Funayama, M. Morikawa
Extremophiles2012, 11 Sep. 2012, English, Poster presentation
[International presentation]

Anammox 細菌Candidatus‘Brocadia sinica’の膜画分に高発現するタンパク質に関する研究　　　　　　　　　　　　　　　
東條ふゆみ, 伊東義兼, 岡部 聡, 森川正章
平成２４年度環境バイオテクノロジー学会（学会奨励賞受賞講演）, 25 Jun. 2012, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Nitrosomonas europaeaのアンモニア酸化活性を促進する因子の同定　　　　　　　　　　　　　　　
折山徹郎, 坂上景子, 三輪京子, 森川正章
平成２４年度環境バイオテクノロジー学会, 25 Jun. 2012, Japanese, Oral presentation
[Domestic Conference]

Beneficial biofilm formation by environmental bacteria　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Invited Seminar at Harvard Medical School, 20 Jun. 2012, English, Invited oral presentation
[Invited], [International presentation]

Analysis of LadA-related long-chain alkane monooxygenase in an extremely thermophilic Geobacillus thermoleovorans B23　　　　　　　　　　　　　　　
C. Boonmak, Y. Takahashi, M. Honma, M. Morikawa
ASM2012, 17 Jun. 2012, English, Poster presentation
[International presentation]

バイオフィルムの形成〜微生物の優れた生存戦略　　　　　　　　　　　　　　　
森川 正章
バイオミメティクス研究会シンポジウム, 07 Dec. 2011, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

海底油田から単離した高度好熱菌Coprothermobacter sp. PM9-2株の諸特性解析　　　　　　　　　　　　　　　
漆畑亘, 船山哲, 森川正章
第12回極限環境生物学会, 27 Nov. 2011, Japanese, Oral presentation
[Domestic Conference]

Enhancement of water purification activity by reconstructing rhizobacterial community　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
JST-NSFC “China-Japan Workshop on Novel Remediation Technologies for Wastewater Environment Conservation", 28 Oct. 2011, English, Invited oral presentation
[Invited], [International presentation]

有益なバイオフィルムの環境技術への利用　　　　　　　　　　　　　　　
森川 正章
日本歯科保存学会2011年度秋季学術大会シンポジウム, 20 Oct. 2011, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Analysis of LadA-related long-chain alkane monooxygenase in an extremely thermophilic Geobacillus thermoleovorans B23　　　　　　　　　　　　　　　
C. Boonmak, Y. Takahashi, M. Morikawa
第63回日本生物工学会大会, 27 Sep. 2011, English, Oral presentation
[Domestic Conference]

Marinobacter属細菌と共存する親属海洋細菌の混合バイオフィルム形成　　　　　　　　　　　　　　　
森川正章, 栄木悠, 柞木田なつみ, 鷲尾健司
第63回日本生物工学会大会, 26 Sep. 2011, Japanese, Oral presentation
[Domestic Conference]

Functional characterization of Thermococcus kodakaraensis S-layer protein in E. coli　　　　　　　　　　　　　　　
T. Agata, W. Urushibata, T. Kanai, H. Atomi, T. Imanaka, M. Morikawa
Thermophiles, 12 Sep. 2011, English, Poster presentation
[International presentation]

Analysis of multiple alkB genes from Geobacillus thermoleovorans B23　　　　　　　　　　　　　　　
C. Boonmak, Y. Takahashi, M. Morikawa
IUMS2011, 09 Sep. 2011, English, Poster presentation
[International presentation]

Purification of azoreductase from a bacterium strain that degrades Orange G dye　　　　　　　　　　　　　　　
S. Murakami, V. Gurning, Y. Itoh, M. Morikawa
IUMS2011, 09 Sep. 2011, English, Poster presentation
[International presentation]

Isolation of an aerobic chemoheterotroph that positively affects the activity of ammonia-oxidizing bacteria　　　　　　　　　　　　　　　
K. Sakagami, Y. Itoh, M. Matsumoto, M. Morikawa
IUMS2011, 09 Sep. 2011, English, Poster presentation
[International presentation]

Isolation and characterization of Rhizobium sp. R8 that forms biofilms on a toilet bowl　　　　　　　　　　　　　　　
T. Fukano, Y. Takahashi, M. Gomi, Y. Osaki, M. Morikawa
IUMS2011, 08 Sep. 2011, English, Poster presentation
[International presentation]

A novel thermotolerant ammonia-oxidizing bacterium, Nitrosomonas thermotolerans JPCCT2, was isolated from activated sludge in a thermal power station in Japan　　　　　　　　　　　　　　　
Y. Itoh, M. Matsumoto, M. Morikawa
IUMS2011, 08 Sep. 2011, English, Poster presentation
[International presentation]

Identification of novel plant-growth promoting factor produced by Acinetobacter calcoaceticus P23, a rhizobacterium of Lemna aoukikusa　　　　　　　　　　　　　　　
S. Chida, M. Morikawa
IUMS2011, 07 Sep. 2011, English, Poster presentation
[International presentation]

An extremely thermophilic bacterium, Coprothermobacter sp. PM9-2, isolated from an offshore petroleum reservoir in the South China Sea　　　　　　　　　　　　　　　
W. Urushibata, T. Funayama, M. Morikawa
国際長期生体学研究ネットワーク（ILTER）年次会議2011, 05 Sep. 2011, English, Keynote oral presentation
[International presentation]

生物界面活性剤と微生物膜　　　　　　　　　　　　　　　
森川 正章
室蘭工業大学工学研究科応用理化学系専攻 講演会, 14 Jul. 2011, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Co-beneficial biofilms on the duckweed roots　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
FEMS 2011 4th Congress of European Microbiologists, 28 Jun. 2011, English, Poster presentation
[International presentation]

原油汚染土壌修復技術へのバイオフィルムの適用　　　　　　　　　　　　　　　
森川正章, 高橋康徳
環境バイオテクノロジー学会2011年度大会, 20 Jun. 2011, Japanese, Poster presentation
[Domestic Conference]

Isolation of a novel thermotolerant ammonia-oxidizing bacterium, Nitrosomonas thermotolerans JPCCT2, from activated sludge in a thermal power station in Japan　　　　　　　　　　　　　　　
Y. Itoh, M. Matsumoto, M. Morikawa
111th ASM General Meeting, 21 May 2011, English, Poster presentation
[International presentation]

Analysis of the granule formation by Anammox bacteria　　　　　　　　　　　　　　　
F. Tojo, Y. Itoh, S. Okabe, M. Morikawa
第一回国際アナモックスシンポジウム, 19 May 2011, English, Public symposium
[International presentation]

好熱性アルカン分解細菌 Geobacillus thremoleovorans B23由来 alkB 遺伝子の多様性解析　　　　　　　　　　　　　　　
高橋康徳, チャニター ブーンマク, 森川正章
日本農芸化学会2011年度大会, 27 Mar. 2011, Japanese, Poster presentation
[Domestic Conference]

耐熱性を有する新規アンモニア酸化細菌の単離ならびに諸性質　　　　　　　　　　　　　　　
伊東義兼, 松本光史, 森川正章
日本農芸化学会2011年度大会, 27 Mar. 2011, Japanese, Poster presentation
[Domestic Conference]

便器表面初期付着細菌群の菌叢解析とバイオフィルム形成　　　　　　　　　　　　　　　
深野透, 高橋康徳, 五味満裕, 大崎幸彦, 森川正章
日本農芸化学会2011年度大会, 27 Mar. 2011, Japanese, Poster presentation
[Domestic Conference]

根圏作用を高度利用した次世代型環境浄化技術の開発　　　　　　　　　　　　　　　
森川 正章
農芸化学会研究企画賞受賞講演, 25 Mar. 2011, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

難分解性アゾ染料Orange Gを分解する細菌およびその還元酵素の精製　　　　　　　　　　　　　　　
村上 峻, グルニング ボルタ, 伊東義兼, 森川正章
生化学会, 10 Dec. 2010, Japanese, Poster presentation
[Domestic Conference]

超好熱始原菌Thermococcus kodakaraensis KOD1由来細胞表層タンパク質Slpを発現する細菌の凝集性およびバイオフィルム形成能の評価　　　　　　　　　　　　　　　
漆畑 亘, 阿形朋子, 金井 保, 跡見晴幸, 今中忠行, 森川正章
生化学会, 07 Dec. 2010, Japanese, Poster presentation
[Domestic Conference]

根圏作用を高度利用した光駆動型バイオ環境浄化技術の開発　　　　　　　　　　　　　　　
森川 正章
北海道大学大学院農学研究院寄付分野講演会, 01 Dec. 2010, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

ウキクサ根圏細菌を利用した持続的水質浄化技術　　　　　　　　　　　　　　　
森川 正章
北海道バイオ産業クラスターフォーラム 技術シーズ公開会, 21 Oct. 2010, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Biofilm formation on the duckweed roots expands substrate specificity of alkane degrading rhizobacteria　　　　　　　　　　　　　　　
M. Morikawa
China-Japan Workshop on Novel Remediation Technologies for Wastewater Environment Conservation, 13 Oct. 2010, English, Nominated symposium
[Invited], [International presentation]

The role of urease activity on biofilm and urolith formation by Staphylococcus sp. T-02 that was isolated from a toilet bowl.　　　　　　　　　　　　　　　
K. Oki, K. Washio, D. Matsui, Y. Hirata, M. Morikawa
IBS2010, 15 Sep. 2010, English, Poster presentation
[International presentation]

Origin of Peroxisomal Beta-oxidation Pathway Suggested by Extremely Thermophilic Alkane Degrading Bacteria　　　　　　　　　　　　　　　
M. Morikawa
BIT’s 1st Annual World Congress of Petromicrobiology, 26 Jul. 2010, English, Invited oral presentation
[Invited], [International presentation]

Enhanced Production of Vitamin K2 by Bacillus subtilis natto Biofilms　　　　　　　　　　　　　　　
M. Morikawa, K. Yamazaki, H. Aibara, K.Washio
BIT’s 3rd Annual World Congress of Industrial Biotechnology, 25 Jul. 2010, English, Invited oral presentation
[Invited], [International presentation]

耐熱性を有する新規アンモニア酸化細菌の単離ならびにその諸性質　　　　　　　　　　　　　　　
伊東義兼, 坂上景子, 松本光史, 鷲尾健司, 森川正章
生化学会北海道支部例会, 23 Jul. 2010, Japanese, Oral presentation
[Domestic Conference]

アンモニア酸化細菌のアンモニア酸化活性に影響を与える従属栄養細菌　　　　　　　　　　　　　　　
坂上景子, 伊東義兼, 松本光史, 鷲尾健司, 森川正章
生化学会北海道支部例会, 23 Jul. 2010, Japanese, Oral presentation
[Domestic Conference]

Sustainable biodegradation of phenol by a symbiotic Acinetobacter calcoaceticus P23 that was isolated from the rhizosphere of duckweed Lemna aoukikusa　　　　　　　　　　　　　　　
F. Yamaga, K. Washio, M. Morikawa
110th ASM General Meeting, 24 May 2010, English, Poster presentation
[International presentation]

(p)ppGppの合成・代謝酵素SpoTによる環状リポペプチドの生産制御　　　　　　　　　　　　　　　
鷲尾健司, リムシューピン, ルーンサワンニラン, 森川正章
日本農芸化学会, 27 Mar. 2010, Japanese, Poster presentation
[Domestic Conference]

フロックを形成するアンモニア酸化細菌の単離ならびにその諸性質　　　　　　　　　　　　　　　
伊東義兼, 松本光史, 鷲尾健司, 森川正章
日本農芸化学会, 27 Mar. 2010, Japanese, Oral presentation
[Domestic Conference]

Nitrosomonas europaeaのアンモニア酸化活性に影響を与える従属栄養細菌　　　　　　　　　　　　　　　
坂上景子, 伊東義兼, 松本光史, 鷲尾健司, 森川正章
日本農芸化学会, 27 Mar. 2010, Japanese, Poster presentation
[Domestic Conference]

ウキクサから単離したアルカン分解根圏細菌の評価　　　　　　　　　　　　　　　
鈴木和也, 山賀文子, 鷲尾健司, 森川正章
日本農芸化学会, 27 Mar. 2010, Japanese, Poster presentation
[Domestic Conference]

Pseudomonas 属細菌の環状リポペプチド生産制御機構　　　　　　　　　　　　　　　
森川 正章
北海道大学シンポジウム「北大の研究者達がつむぐ微生物の世界」, 08 Dec. 2009, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

ウキクサと根圏細菌の相利共生作用による汚染浄化法　　　　　　　　　　　　　　　
森川正章, 鷲尾健司
水処理生物学会, 12 Nov. 2009, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Characterization of crude oil degrading rhizobacteria　　　　　　　　　　　　　　　
Kazuya SUZUKI
Summary of 12th HU-SNU Joint Symposium, 12 Nov. 2009, English, Public symposium
[International presentation]

超好熱性アーキアThermococcus kodakaraensis KOD1由来細胞表在タンパク質Slpの機能解析　　　　　　　　　　　　　　　
阿形朋子, 鷲尾健司, 金井 保, 跡見晴幸, 今中忠行, 森川正章
生化学会, 21 Oct. 2009, Japanese, Poster presentation
[Domestic Conference]

火力発電所硝化槽より単離した新規メタノール資化細菌の諸特性解析　　　　　　　　　　　　　　　
石川絵里奈, 伊東義兼, 鷲尾健司, 松本光史, 森川正章
生化学会, 21 Oct. 2009, Japanese, Poster presentation
[Domestic Conference]

Biofilm formation and effective production of menaquinone-7 (Vitamin K2) by Bacillus subtilis: Lost of the world in environmental bacteria.　　　　　　　　　　　　　　　
Kenji Washio, Masaaki Morikawa
Workshop Argentina-Japan “Bioscience and Biotechnology for the Promotion of Agriculture and Food Production”, 03 Aug. 2009, English, Invited oral presentation
[Invited], [International presentation]

Studies on a lipopeptide biosurfactant, arthrofactin – structure, biosynthesis, transportation　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Invited lecture at UC Berkeley, California,, 01 Aug. 2009, English, Invited oral presentation
[Invited], [International presentation]

バイオフィルムに見られるナフタレン分解活性遅延の理由　　　　　　　　　　　　　　　
嶋田恒平, 鷲尾健司, 森川正章
環境バイオテクノロジー学会, 23 Jun. 2009, Japanese, Poster presentation
[Domestic Conference]

微生物によるジクロロメタン分解に関する研究　　　　　　　　　　　　　　　
岩崎一弘, 中杉奈央, 大川恵, 菅田曜, 森川正章, 宮崎英男, 坂間至朗
環境バイオテクノロジー学会, 23 Jun. 2009, Japanese, Poster presentation
[Domestic Conference]

Genetic analysis of synthetic regulation and transportation of lipopeptide type biosurfactant.　　　　　　　　　　　　　　　
N. Roongsawang, S. P. Lim, K. Washio, M. Morikawa
Biosurfactant workshop, 26 Apr. 2009, English, Invited oral presentation
[Invited], [International presentation]

微生物から見た界面の世界—バイオサーファクタントとバイオフィルム　　　　　　　　　　　　　　　
森川 正章
日本化粧品技術者会, 17 Apr. 2009, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

バイオフィルム利用の将来展望　　　　　　　　　　　　　　　
森川 正章
農芸化学会大会, 28 Mar. 2009, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Anammox細菌のグラニュール形成に関するプロテオミック解析　　　　　　　　　　　　　　　
東條ふゆみ, 伊東義兼, 鷲尾健司, 岡部聡, 森川正章
農芸化学会大会, 27 Mar. 2009, Japanese, Poster presentation
[Domestic Conference]

根圏細菌Acinetobacter sp. P23株のバイオフィルム形成遺伝子の探索　　　　　　　　　　　　　　　
羽山亨, 山賀文子, 鷲尾健司, 森川正章
農芸化学会大会, 27 Mar. 2009, Japanese, Poster presentation
[Domestic Conference]

バイオフィルム形成能の高いアルカン分解細菌の探索　　　　　　　　　　　　　　　
高橋康徳, 嶋田恒平, 鷲尾健司, 森川正章
農芸化学会大会, 27 Mar. 2009, Japanese, Poster presentation
[Domestic Conference]

枯草菌バイオフィルムによるメナキノンの菌体外生産　　　　　　　　　　　　　　　
山崎和彦, 相原悠, 大胡康, 鷲尾健司, 森川正章
農芸化学会大会, 27 Mar. 2009, Japanese, Poster presentation
[Domestic Conference]

Sustainable Bioremediation Technology by Utilizing Biofilms　　　　　　　　　　　　　　　
Morikawa, F. Yamaga, K. Shimada, K. Washio
International symposium on Biotechnological Approaches to Environmental Science for Energy Production and Sustainability., 25 Feb. 2009, English, Invited oral presentation
[Invited], [International presentation]

環状リポペプチド、アルスロファクチンの産生を制御する (p)ppGpp合成・代謝酵素、SpoTの機能解析　　　　　　　　　　　　　　　
鷲尾健司, リムシューピン, ルーンサワンニラン, 森川正章
日本分子生物学会•日本生化学会合同大会2008, 09 Dec. 2008, Japanese, Poster presentation
[Domestic Conference]

Staphylococcus 属細菌のバイオフィルム形成における尿素分解酵素（ウレアーゼ）の役割　　　　　　　　　　　　　　　
大木海平, 松井大悟, 平田善彦, 鷲尾健司, 森川正章
日本分子生物学会•日本生化学会合同大会2008, 09 Dec. 2008, Japanese, Poster presentation
[Domestic Conference]

バイオフィルム研究の様々な切り口ー分子、細胞、生態ー　　　　　　　　　　　　　　　
森川 正章
日本微生物生態学会２０年度大会, 27 Nov. 2008, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Advances in Biofilm Research to Inhibit Biocorrosion　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
ARO workshop, 22 Sep. 2008, English, Invited oral presentation
[Invited], [International presentation]

新規アゾ染料分解脱色細菌の探索　　　　　　　　　　　　　　　
文 Volta, R. T. Gurning, 鷲尾健司, 森川正章
環境バイオテクノロジー学会２０年度大会, 25 Jun. 2008, Japanese, Poster presentation
[Domestic Conference]

Efficacy of biofilm formation by naphthalene degrading Pseudomonas stutzeri T102 toward bioremediation technology.　　　　　　　　　　　　　　　
ASM 108th, 01 Jun. 2008, English, Poster presentation
[International presentation]

Biofilm formation by Bacillus subtilis 168, incompatible function of sfp　　　　　　　　　　　　　　　
H. Aibara, K. Washio, M. Morikawa
ASM 108th, 01 Jun. 2008, English, Poster presentation
[International presentation]

A lipopeptide biosurfactant produced by Pseudomonas sp. MIS38, characterization and functional analyses of the synthetase gene.　　　　　　　　　　　　　　　
M. Morikawa
Invited lecture at East China University of Science and Technology, 20 May 2008, English, Invited oral presentation
[Invited], [International presentation]

Efficacy of biofilm formation by naphthalene degrading Pseudomonas stutzeri T102 toward bioremediation technology.　　　　　　　　　　　　　　　
M. Morikawa, K. Shimada, K. Washio
iBIO2008, 19 May 2008, English, Invited oral presentation
[Invited], [International presentation]

小便器内壁から単離した細菌のバイオフィルム形成能　　　　　　　　　　　　　　　
大木海平, 松井大悟, 平田善彦, 鷲尾健司, 森川正章
農芸化学会, 27 Mar. 2008, Japanese, Poster presentation
[Domestic Conference]

Pseudomonas stutzeri バイオフィルムのナフタレン分解活性と土壌安定性　　　　　　　　　　　　　　　
嶋田恒平, 片岡剛文, 鷲尾健司, 森川正章
農芸化学会, 27 Mar. 2008, Japanese, Poster presentation
[Domestic Conference]

超好熱性アーキアThermococcus kodakaraensis KOD1 の浮遊細胞とコロニー細胞を用いたプロテオーム解析　　　　　　　　　　　　　　　
阿形朋子, 松見理恵, 鷲尾健司, 金井保, 跡見晴幸, 今中忠行, 森川正章
農芸化学会, 27 Mar. 2008, Japanese, Poster presentation
[Domestic Conference]

食中毒原因細菌のバイオフィルムと浮遊細胞の加熱処理耐性比較　　　　　　　　　　　　　　　
東條ふゆみ, 佐藤 禅, 鷲尾健司, 森川正章
農芸化学会, 27 Mar. 2008, Japanese, Poster presentation
[Domestic Conference]

Pseudomonas sp. MIS38株由来の二つの環状リポペプチド類ABC-トランスポーター　　　　　　　　　　　　　　　
Siew Ping Lim, Niran Roongsawang, 鷲尾健司, 森川正章
生化学会, 12 Dec. 2007, English, Poster presentation
[Domestic Conference]

フタル酸エステル分解細菌の単離と諸特性解析　　　　　　　　　　　　　　　
菅原寛明, 陳 志銘, 鷲尾健司, 森川正章
生化学会, 12 Dec. 2007, Japanese, Poster presentation
[Domestic Conference]

Pseudomonas sp. MIS38株の成長相とバイオサーファクタント生産　　　　　　　　　　　　　　　
鷲尾健司, Siew-Ping Lim, Roongsawang Niran, 森川正章
生化学会, 12 Dec. 2007, Japanese, Poster presentation
[Domestic Conference]

バイオフィルムってなんだろう　　　　　　　　　　　　　　　
森川 正章
地球環境科学研究院公開講座「快適な環境をまもる微生物のはたらきと姿」, 25 Sep. 2007, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

微生物の生存戦略：バイオフィルムとバイオサーファクタント　　　　　　　　　　　　　　　
森川 正章
日清製粉基礎研究所セミナー, 14 Jul. 2007, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

環境微生物の生存戦略ーバイオフィルムー　　　　　　　　　　　　　　　
森川正章, 相原 悠, 山崎和彦, 鷲尾健司
生化学会北海道支部シンポジウム, 06 Jul. 2007, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Pseudomonas stutzeri バイオフィルムのナフタレン分解活性と土壌安定性　　　　　　　　　　　　　　　
嶋田恒平, 片岡剛文, 鷲尾健司, 森川正章
環境バイオテクノロジー学会, 26 Jun. 2007, Japanese, Oral presentation
[Domestic Conference]

細菌バイオフィルムと浮遊細胞の耐熱性比較　　　　　　　　　　　　　　　
東條ふゆみ, 佐藤 禅, 鷲尾健司, 森川正章
環境バイオテクノロジー学会, 26 Jun. 2007, Japanese, Poster presentation
[Domestic Conference]

フタル酸エステル分解細菌の単離と諸特性解析　　　　　　　　　　　　　　　
菅原寛明, 陳 志銘, 鷲尾健司, 森川正章
環境バイオテクノロジー学会, 26 Jun. 2007, Japanese, Poster presentation
[Domestic Conference]

非リボソーム型ペプチド合成酵素の解析と機能改変　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
第8回 酵素応用シンポジウム, 15 Jun. 2007, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Genes Responsible for the Transportation of Arthrofactin (Arf) in Pseudomonas sp. MIS38　　　　　　　　　　　　　　　
Lim SP, Roongsawang N, Washio K, Morikawa M
ASM 107th Meeting, 22 May 2007, English, Poster presentation
[International presentation]

バイオフィルムとバイオサーファクタント　　　　　　　　　　　　　　　
森川 正章
日清製粉研究所セミナー, 23 Apr. 2007, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

水生植物からの汚染物質分解能を有する微生物の探索　　　　　　　　　　　　　　　
山賀 文子, 竹井 大亮, 鷲尾 健司, 森川 正章
農芸化学会, 26 Mar. 2007, Japanese, Oral presentation
[Domestic Conference]

海洋性細菌を用いた複数種バイオフィルム形成能の評価　　　　　　　　　　　　　　　
柞木田 なつみ, 岡田 千尋, 鷲尾 健司, 森川 正章
農芸化学会, 26 Mar. 2007, Japanese, Oral presentation
[Domestic Conference]

枯草菌バイオフィルム形成に与えるサーファクチン生産の影響　　　　　　　　　　　　　　　
相原 悠, 鷲尾 健司, 森川 正章
農芸化学会, 26 Mar. 2007, Japanese, Oral presentation
[Domestic Conference]

Pseudomonas sp. MIS38株が産生する環状リポペプチドの生成機構の解析　　　　　　　　　　　　　　　
鷲尾 健司, Lim Siew-Ping, Roongsawang Niran, 柞木田 なつみ, 森川正章
農芸化学会, 25 Mar. 2007, Japanese, Oral presentation
[Domestic Conference]

人に役立つ微生物のちから　　　　　　　　　　　　　　　
森川 正章
ヤクルト講演会, 18 Nov. 2006, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

枯草菌のバイオフィルム形成に関する遺伝子と分子　　　　　　　　　　　　　　　
森川 正章
日本農芸化学会北海道支部ミニシンポジウム『クオルモン利用の最前線』, 11 Nov. 2006, Japanese, Nominated symposium
[Invited], [Domestic Conference]

Isolation of biofilm-forming bacteria from marine environments　　　　　　　　　　　　　　　
M. Morikawa, C. Okada, K. Washio, K. Takano, S. Kanaya
SGM 159th Meeting, 11 Sep. 2006, English, Oral presentation
[International presentation]

Isolation and characterization of useful bacteria for bioremediation.　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Tong Hai University-Hokkaido University Joint seminar, 25 Aug. 2006, English, Oral presentation
[International presentation]

Isolation and characterization of bacteria that degrade DEHP.　　　　　　　　　　　　　　　
C-M. Chen, K. Washio, K. Takano, S. Kanaya, M. Morikawa
Tong Hai University-Hokkaido University Joint seminar, 21 Aug. 2006, English, Oral presentation
[International presentation]

バクテリアの個性と社会性 —微生物群集への新たな理解を求めてー「枯草菌のバイオフィルム形成機構」　　　　　　　　　　　　　　　
森川 正章
第16回 根圏ネットワーク講演会『バクテリアの個性と社会性』, 02 Aug. 2006, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

疎水環境と微生物　　　　　　　　　　　　　　　
森川 正章
バイオウィーク in Sapporo 2006, 04 Jul. 2006, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

特殊環境微生物の最新研究動向と産業利用「疎水環境と微生物」　　　　　　　　　　　　　　　
森川 正章
バイオウィーク in Sapporo, 04 Jul. 2006, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

海洋細菌による混合バイオフィルム形成能の評価　　　　　　　　　　　　　　　
柞木田なつみ, 岡田千尋, 鷲尾健司, 森川正章
環境バイオテクノロジー学会, 27 Jun. 2006, Japanese, Poster presentation
[Domestic Conference]

A Novel Biofilm-Forming Rhizobiales Isolated from Seto Inland Sea　　　　　　　　　　　　　　　
C. Okada, K. Washio, K. Takano, S. Kanaya, M. Morikawa
ASM 106th Meeting, 21 May 2006, English, Poster presentation
[International presentation]

Characterization of the C-terminal thioesterase domains in arthrofactin synthetase: a prototype of two internal thioesterases　　　　　　　　　　　　　　　
Niran Roongsawang, Kenji Washio, Masaaki Morikawa
SGM 158th Meeting, 03 Apr. 2006, English, Oral presentation
[International presentation]

瀬戸内海バイオフィルムから単離された新属細菌の報告　　　　　　　　　　　　　　　
岡田千尋, 金谷茂則, 森川正章
農芸化学会, 26 Mar. 2006, Japanese, Poster presentation
[Domestic Conference]

P12 Structural analysis of a biosurfactant, arthrofactin, produced by Pseudomonas sp. MIS38　　　　　　　　　　　　　　　
近藤 泰史, 川上 徹, 森川 正章, 池上 貴久
産業用酵素国際シンポジウム, 15 Feb. 2006, Japanese, Oral presentation
[Domestic Conference]

Determination of the conformation and configuration of arthrofactin by NMR　　　　　　　　　　　　　　　
近藤泰史, 池上貴久, 森川正章
日本生物物理学会, 23 Nov. 2005, Japanese, Oral presentation
[Domestic Conference]

バイオフィルムの成因と生存メカニズム　　　　　　　　　　　　　　　
森川 正章
第12回HACCP・微生物制御に関する講演会, 08 Sep. 2005, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Phylogenetic analysis of condensation domain in nonribosomal peptide synthetases suggests the evolutionary relationship in the peptide donor molecules　　　　　　　　　　　　　　　
NIRAN ROONGSAWANG, KAZUFUMI TAKANO, SHIGENORI KANAYA, MASAAKI MORIKAWA
SGM2005, 07 Apr. 2005, English, Oral presentation
[International presentation]

Identification of epimerase domain in non-ribosomal peptide synthetases (NRPSs) of biosurfactant producing Pseudomonas sp. MIS38　　　　　　　　　　　　　　　
Siew Ping Lim, Niran Roongsawang, Kenji Washio, Kazufumi Takano, Shigenori Kanaya, Masaaki Morikawa
日本農芸化学会2005年大会, 28 Mar. 2005, English, Oral presentation
[Domestic Conference]

Phylogenetic analysis of condensation domain in nonribosomal peptide synthetases suggestes the evolutionary relationship in the peptide donor molecules　　　　　　　　　　　　　　　
Niran Roongsawang, Kazufumi Takano, Shigenori Kanaya, Masaaki Morikawa
日本農芸化学会2005年大会, 28 Mar. 2005, English, Oral presentation
[Domestic Conference]

Studies on Gordonia sihwensis C4 that degrade plasticizer (DEHP)　　　　　　　　　　　　　　　
陳 志銘, 森川正章, 高野和文, 金谷茂則
日本農芸化学会2005年大会, 28 Mar. 2005, English, Oral presentation
[Domestic Conference]

Studies on the biofilm formation by a wild-type Bacillus subtilis　　　　　　　　　　　　　　　
森川 正章, 金谷茂則, 今中忠行
日本生物工学会, 15 Nov. 2004, Japanese, Invited oral presentation
[Domestic Conference]

Analyses on the alkane degrading genes of Rhodococcus sp. TMP2　　　　　　　　　　　　　　　
竹井大亮, 国広波緒, 高野和文, 金谷茂則, 森川正章
日本生物工学会, 15 Nov. 2004, Japanese, Oral presentation
[Domestic Conference]

Biofilm formation and protease production by Pseudoalteromonas sp. SB-B1　　　　　　　　　　　　　　　
森川 正章, 飯島沙織, 岡原良太, 高野和文, 金谷茂則
日本生物工学会, 15 Nov. 2004, Japanese, Oral presentation
[Domestic Conference]

好熱性油田細菌と超好熱菌に見る生命の渾沌とした姿　　　　　　　　　　　　　　　
森川 正章
日本植物学会北日本支部大会, 04 Sep. 2004, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

バイオフィルム　　　　　　　　　　　　　　　
森川 正章
サラヤ株式会社セミナー, 19 Dec. 2003, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Complete sequence of arthrofactin biosynthesis operon with unique two carboxy-terminal thioesterase domains.　　　　　　　　　　　　　　　
N. Roongsawang, M. Haruki, T. Imanaka, M. Morikawa, S. Kanaya
ASM 103th, 17 May 2003, English
[International presentation]

Terrahaemophilus aromaticivorans gen nov. sp. nov., a terrestrial Pasteurellaceae isolated from petroleum sludge.　　　　　　　　　　　　　　　
S. Hirano, M. Haruki, T. Imanaka, M. Morikawa, S. Kanaya
ASM 103th, 17 May 2003, English
[International presentation]

Oleomonas sagaranensis gen. nov., sp. nov., represents a novel genus in the alpha-Proteobacteria.　　　　　　　　　　　　　　　
T. Kanamori, N. Rashid, M. Morikawa, H. Atomi, T. Imanaka
ASM 103th, 17 May 2003, English
[International presentation]

Structural analysis of extracellular polymeric substances (EPS) from Bacillus subtilis that forms robust pellicles.　　　　　　　　　　　　　　　
M. Morikawa, M. Kagihiro, T. Imanaka, R. Kolter, S. Kanaya
ASM 103th, 17 May 2003, English
[International presentation]

生物の過酷環境への適応戦略ー低温適応を中心として「バイオフィルム形成と環境適応」　　　　　　　　　　　　　　　
森川 正章
大阪大学蛋白質研究所セミナー, 11 Mar. 2003, Japanese
[Domestic Conference]

Site-specific cleavage of MS2 RNA by a thermostable DNA-linked RNase H.　　　　　　　　　　　　　　　
C. Hyongi, T. Nogawa, Y. Tsunaka, M. Haruki, M. Morikawa, S. Kanaya
RNase H International Conference 2002, 24 Sep. 2002, English
[International presentation]

Structural and functional analyses of archaeal Type 2 ribonuclease H.　　　　　　　　　　　　　　　
A. Muroya, D. Tsuchiya, M. Ishikawa, M. Haruki, M. Morikawa, S. Kanaya, K. Morikawa
RNase H International Conference 2002, 24 Sep. 2002, English
[International presentation]

Cleavage of a double-stranded DNA containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII　　　　　　　　　　　　　　　
M. Haruki, Y. Tsunaka, M. Morikawa, S. Kanaya
RNase H International Conference 2002, 24 Sep. 2002, English
[International presentation]

環境に役立つ微生物たち　　　　　　　　　　　　　　　
森川 正章
ボストン日本人研究者交流会, 12 Jan. 2002, Japanese, Invited oral presentation
[Invited]

The world of extremophilic oil bacteria　　　　　　　　　　　　　　　
MORIKAWA, Masaaki
Seminar at Department of Molecular and Cellular Biology, Harvard University, 08 Jul. 2001, English, Invited oral presentation
[Invited], [International presentation]

Long-chain-alkane degradation by extremely thermophilic Bacteria.　　　　　　　　　　　　　　　
M. Morikawa, T. Kato, M. Haruki, T. Imanaka, S. Kanaya
ASM 101th, 20 May 2001, English
[International presentation]

環境バイオテクノロジー：基礎研究の戦略と事業化への展開　　　　　　　　　　　　　　　
森川 正章
日本化学工学会シンポジウム, 02 Apr. 2001, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Biological oil production from CO2.　　　　　　　　　　　　　　　
T. Imanaka, M. Morikawa
Pacifichem 2000, 14 Dec. 2000, English
[International presentation]

Gene cloning and characterization of an aldehyde dehydrogenase and an esterase from a petroleum degrading strain HD-1.　　　　　　　　　　　　　　　
M. Morikawa, S. Mizuguchi, N. Okibe, M. Haruki, T. Imanaka, S. Kanaya
Pacifichem 2000, 14 Dec. 2000, English
[International presentation]

An extreme-thermophile which degrades petroleum under high temperature conditions.　　　　　　　　　　　　　　　
T. Kato, A. Miyanaga, M. Haruki, T. Imanaka, M. Morikawa, S. Kanaya
Pacifichem 2000, 14 Dec. 2000, English
[International presentation]

Thermophilic bacteria isolated from a subterranean petroleum reservoir.　　　　　　　　　　　　　　　
M. Morikawa, T. Kato, M. Haruki, T. Imanaka, S. Kanaya
Gratama Workshop 2000 conference, 21 Apr. 2000, English
[International presentation]

CO2 fixation and alkane production by an oil-bacterium strain HD-1.　　　　　　　　　　　　　　　
M. Morikawa, H. Atomi, T. Imanaka
International Symposium on Autotrophy and Hydrogen Biotechnology, 14 Feb. 2000, English, Invited oral presentation
[Invited], [International presentation]

CO2固定細菌を利用した地球環境修復システムの構築　　　　　　　　　　　　　　　
森川 正章
近畿バイオインダストリー振興会議第一回技術シーズ公開会, 28 Sep. 1999, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

資源をつくる微生物　　　　　　　　　　　　　　　
森川 正章
日本農芸化学会ミニシンポジウム, 11 Jul. 1998, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Isolation of a petroleum degrading/producing bacterium　　　　　　　　　　　　　　　
M. Morikawa, S. Jin, K. Amada, M. Haruki, S. Kanaya, T. Imanaka
International Congress on Extremophiles '98, 18 Jan. 1998, English
[International presentation]

Petroleum degrading/producing bacterium HD-1 with its unique characters.　　　　　　　　　　　　　　　
M. Morikawa, S. Kanaya, T. Imanaka
Asian Nitrogen-Fixation Symposium under JSPS and Monbusho Programme, 08 Dec. 1997, English
[International presentation]

A new type of petroleum-degrading/producing bacterium HD-1.　　　　　　　　　　　　　　　
M. Morikawa, T. Imanaka
International Workshop on Ultra-long term Cryogenic Preservation Network of Biological and Environmental Specimens, 03 Nov. 1997, English
[International presentation]

Production of alkane and alkene from CO2 by a petroleum-degrading bacterium strain HD-1.　　　　　　　　　　　　　　　
M. Morikawa, T. Iwasa, S. Yanagida, T. Imanaka
Fourth International Conference on Carbon Dioxide Utilization, 08 Sep. 1997, English
[International presentation]

Studies on the structure-function relationship of lipopeptide biosurfactants.　　　　　　　　　　　　　　　
M. Morikawa, T. Imanaka
International Society for Environmental Biotechnology 3rd International Symposium, 15 Jul. 1996, English
[International presentation]

A new mixotrophic bacterium which can fix CO2 and assimilate aliphatic and aromatic hydrocarbons anaerobically.　　　　　　　　　　　　　　　
T. Imanaka, M. Morikawa
International Society for Environmental Biotechnology 3rd International Symposium, 15 Jul. 1996, English
[International presentation]

「極限環境における生体の分子論的応答」有機溶媒：バイオサーファクタントと石油代謝細菌　　　　　　　　　　　　　　　
森川 正章
大阪大学蛋白質研究所セミナー, 26 Jan. 1996, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

ヒト・リゾチームの構造・機能相関解析　　　　　　　　　　　　　　　
森川 正章
生物工学若手研究者の集い, 26 Jul. 1989, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

平成23年度北海道大学大学院地球環境科学研究院公開講座 「生物の環境への適応」　　　　　　　　　　　　　　　
2011

オープンキャンパス2011 （北大理学部）　　　　　　　　　　　　　　　
2011

プロフェッサービジット2009　　　　　　　　　　　　　　　
2010

プロフェッサービジット2010　　　　　　　　　　　　　　　
2010

オープンキャンパス2010（北大理学部）　　　　　　　　　　　　　　　
2010

ボストン日本人研究者交流会（於，マサチューセッツ工科大学） 「環境に役立つ微生物たち　　　　　　　　　　　　　　　
2002

日本化学工学会シンポジウム 環境バイオテクノロジー：基礎研究の戦略と事業化への展開 展望講演「CO2固定細菌と油田細菌の環境修復技術への可能性」　　　　　　　　　　　　　　　
2001

International Symposium on Autotrophy and Hydrogen Biotechnology （於，東京YMCA）"CO2 fixation and alkane production by an oil-bacterium strain HD-1."　　　　　　　　　　　　　　　
2000

近畿バイオインダストリー振興会議第一回技術シーズ公開会 （於，大阪科学技術センター） 「CO2固定細菌を利用した地球環境修復システムの構築　　　　　　　　　　　　　　　
1999

日本農芸化学会ミニシンポジウム（於，大阪府立大学） 「資源をつくる微生物」　　　　　　　　　　　　　　　
1998

Elucidation of holobiont co-evolution mechanism by analyzing aquatic plant-surface symbiotic bacteria interactions
Grants-in-Aid for Scientific Research
09 Jul. 2021 - 31 Mar. 2024
森川 正章
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Pioneering), Hokkaido University, 21K18239

Development of the duckweed holobiont resource values towards Thailand BCG economy
Science and Technology Research Partnership for Sustainable Development
Aug. 2020
Masaaki Morikawa
Japan Science and Technology Agency/Japan Internationas Cooperation Agency, Hokkaido Univ., Kyoto Univ., Osaka Univ., Tohoku Univ., Univ. Yamanashi, AIST, Principal investigator

Effective Aquatic Biomass Production Utilizing Mutualistic Microorganisms
Advanced Low Carbon Technology and Research Development
Sep. 2016 - Mar. 2020
MORIKAWA, Masaaki
Japan Science and Technology Agency, Hokkaido Univ., Kyoto Univ., Osaka Univ., Tohoku Univ., Univ. Yamanashi, AIST, Principal investigator, Competitive research funding

新奇な水生植物ー微生物相互作用の発見と理解
挑戦的萌芽研究
Apr. 2016 - Mar. 2018
森川 正章
JSPS:科学研究補助金, Principal investigator, Competitive research funding

Application of Rhizosphere Microorganisms Symbiosis to Highly Ordered Plant BIoprocesses
Advanced Low Carbon Technology Research and Development Program
Oct. 2011 - Aug. 2016
Masaaki Morikawa
Japan Science and Technology Agency, Hokkaido Univ., Osaka Univ., Univ. Yamanashi, Kitasato Univ., AIST, Principal investigator, Competitive research funding

高度好熱菌性油田細菌のアルカン代謝および生態に関する研究　　　　　　　　　　　　　　　
科学研究費基盤B一般
2010 - 2012
森川 正章
JSPS, Principal investigator, Competitive research funding

バイオフィルム工学による微生物のデザイン化　　　　　　　　　　　　　　　
受託研究「微生物機能を活用した環境調和型製造基盤技術開発」
2007 - 2011
森川 正章
NEDO, Principal investigator, Competitive research funding

バイオフィルム形成分子機構を切り口とした微生物未知機能の解明　　　　　　　　　　　　　　　
科学研究費基盤B一般
2007 - 2009
森川 正章
JSPS, Principal investigator, Competitive research funding

バイオフィルムを利用した環境修復技術の開発　　　　　　　　　　　　　　　
発酵研究所研究助成
2006 - 2008
森川 正章
（財）発酵研究所, Principal investigator, Competitive research funding

寒冷条件に対応した次世代汚染土壌修復技術の開発　　　　　　　　　　　　　　　
受託研究 （中小企業ベンチャー挑戦支援事業)
2008
森川 正章
METI, Principal investigator, Competitive research funding

非リボソーム型ペプチド合成酵素の解析と機能改変　　　　　　　　　　　　　　　
酵素応用シンポジウム奨励賞
2007
森川 正章
（株）天野エンザイム, Principal investigator, Competitive research funding

脂溶性機能性食品成分の菌体外分泌システム開発　　　　　　　　　　　　　　　
食生活研究会研究助成
2007
森川 正章
（財）食生活研究会, Principal investigator, Competitive research funding

バイオフィルム工学を利用した生物環境修復技術の開発　　　　　　　　　　　　　　　
共同研究 （J-Power ５０周年記念先端技術共同研究)
2005 - 2006
森川 正章
J-Power, Principal investigator, Competitive research funding

Arthrofactin 合成反応の分子メカニズム　　　　　　　　　　　　　　　
科学研究費基盤C一般
2005 - 2006
森川 正章
JSPS, Principal investigator, Competitive research funding

病原微生物データ分析実験作業　　　　　　　　　　　　　　　
受託研究 食品製造工程管理情報高度化促進事業
2006
森川 正章
（財）食品産業センター, Principal investigator, Competitive research funding

新規リボソーム非依存型ペプチド合成酵素の構造・機能相関に関する研究
科学研究費助成事業
2004 - 2005
森川 正章, ROONGSAWANG Niran
非リボソーム型ペプチド合成酵素は生理活性のある非常に多様な複合/環状ペプチド類を合成することができる。合成最終段階の環状化反応と産物の遊離は合成酵素のC末端に存在するチオエステラーゼドメイン(TE)の働きにより進行する。Pseudomonas属細菌MIS38が有するarthrofactin合成酵素は3つのサブユニット(ArfA/B/C)から構成される。興味深いことにArfCのC末端には2つのTEが存在する。いずれのTE(TE1,TE2)も活性発現に必須のSr/Asp/Hisを有している。本研究では、これらのTEが合成反応にどのように係わっているのかを解明することを目的とした。
当該遺伝子部分を大腸菌用ベクターを用いてクローニングした後、PCR法を用いて部位特異的突然変異遺伝子を構築した。さらにこの変異遺伝子を相同性組み換え法によりMIS38染色体に戻した。こうして、S91A,S91T,S383A,S383/D410A変異株を作製した。塩基配列解析を行い、それぞれの位置以外に突然変異が導入されていないことを確認した。こうして得られた変異株のarthrofactin生産性を調べた。その結果、S91A,S91T変異株は生産性を完全に失っていた。一方、S383AおよびS383A/D410A変異株は野生株に比べて5±1%の活性を保持していた。またこれら変異株が生産するarthrofactinは野生株のものと同一の構造であることを確認した。以上の結果から、S91を含むTE1が環状化反応と産物の遊離に必須であり、TE2は本質的に重要ではないことが判明した。現在,この仮説を証明するためにTE2を完全に決失した変異株を作成中である。
日本学術振興会, 特別研究員奨励費, 北海道大学, 04F04468

汚染土壌微生物生態を利用した環境修復技術の開発　　　　　　　　　　　　　　　
2005
森川 正章
住友財団, Principal investigator, Competitive research funding

革新的リボソーム非依存型複合ペプチド合成酵素に関する研究　　　　　　　　　　　　　　　
武田科学振興財団奨励金一般研究
2005
森川 正章
武田科学振興財団, Principal investigator, Competitive research funding

カラー空間符号モアレマッチング法によるゲノム情報における規則性の抽出
科学研究費助成事業
2003 - 2003
谷田 純, 森川 正章
空間符号モアレマッチング技術を基本としたゲノム解析手法の開発を通して,その有効性,特牲,問題点を明らかにするとともに,ゲノム情報処理分野における情報フォトニクス技術応用の可能性を探ることを目的とする.本年度に得られた知見は以下の通りである.
1.昨年度考案したカラー空間符号モアレマッチング技術を実装したプログラム(MTerm)により,各種細菌類を対象とした全ゲノム比較を行い,有効性,特性,問題点を評価した.ほとんどの比較において一様なモアレ縞が観測され,モアレ縞が観測されないほうがむしろ稀であることを確認した.また,出現頻度符号化を用いた場合,区切り枠の取り方により一致線が分裂する現象を見いだし,その対策として,MTermに区切り枠の調整機構を付加した.
2.ゲノム配列内に存在する逆位の検出性に対する検討を行った.配列塩基種の相補塩基への変換,塩基の配置方向の反転などの機能をMTerm付加した.大腸菌O157EDL933株と同sakai株に適用し,配列多重度10000においても逆位部位の検出が行えることを確認した.
3.配列一致部位の自動検出をめざして,出力モアレ縞画像に対する特徴抽出処理の検討を行った.移動平均法の多重適用,閾値処理による二値化,膨張・収縮処理によるノイズ成分除去の一連の処理により,良好な特徴抽出結果が得られることを確認した.さらに,処理画像に対する情報圧縮により,1/500程度の圧縮率が得られることを確認した.
日本学術振興会, 特定領域研究, 大阪大学, 15014219

Development of heat-labile and psychrophilic enzymes for clean and efficient recombinant DNA experiment
Grants-in-Aid for Scientific Research
2002 - 2003
HARUKI Mitsuru, MORIKAWA Masaaki
We tried to develop heat-labile alkaline phosphatase, BglII, and RNase A to inactivate these enzymes by heat in recombinant DNA experiment, and to isolate psychrophilic DNA ligase gene for efficient DNA ligation at low temperatures.
An alkaline phosphatase (APase) from psychrotrophic bacterium Shewanella sp. SIBI was overproduced in E. coli, purified, and characterized. The enzyme exhibited the highest activity at 40℃. This optimum temperature is shifted downward by 20℃, as compared to that of E. coli APase. SIBI APase was almost fully inactivated upon incubation at 80℃. for 5min, whereas E. coli APase retained 60% of the activity upon incubation at 80℃ for 1h. The k_ value of SIBI APase was higher than that of E. coli APase by 6.2 times at 40℃ and 9.6 times at 20℃.
A part of DNA ligase gene was isolated from Shewanella sp. SIB1. A pair of PCR primers was designed based on the DNA sequence of the DNA ligase gene of Shewanella oneidensis MR-1, which is closely related to Shewanella sp. SIB1. A 600-bp DNA fragment was amplified by PCR using MR-1 genomic DNA as a template and the designed primers. The DNA fragment encoded an amino acid sequence with 77% identity to MR-1 DNA ligase. Isolation of the entire gene is now under progress.
To select heat-labile mutant of Bgl II and RNase A, we tried to display these enzymes on M13 phage by inserting the genes of these enzymes with a 6 × His-tag at their N-termini to the 5'-terminus of the coat protein pIII gene. Phagemid particles were produced from E. coli strains harboring these hybrid genes, and were analyzed for the binding to a Ni-NTA plate. However, no specific binding was observed, possibly because phage display of these enzymes was unsuccessful.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Nihon University, 14550769

空間符号モアレマッチング法によるマルチスケールゲノム情報解析
科学研究費助成事業
2002 - 2002
谷田 純, 森川 正章
空間符号化法を用いた配列比較技術に対して,符号化法の改良や専用視覚化端末の開発を通し,全ゲノム配列比較への拡張手法に関する種々の検討を行った.また,これらの手法を各種大腸菌の全ゲノムやヒト染色体などの実配列に適用し,その有効性を評価した.その結果,以下に示す成果を得た.
1.単一ディスプレイ上でマッチング処理を実行するためのソフトウェアを開発し,これまでに考案した種々の符号化法を実装した.さらに,符号化画像の更新や選択配列部位の切り出しなどの様々な機能を付加させることにより,専用視覚端末の利便性を向上させた.
2.長大な2配列間のマッチング結果を同時に提示するため,対象配列を一定の長さを有する部分配列に分割し,部分配列内の各塩基要素を符号パターンに反映させる出現頻度符号化法を考案した.この符号化法を用いることで,数10万塩基長に及ぶ2配列間のマッチング結果を同時に表示できることを確認した.
3.出現頻度符号化法により得られる出力結果から,配列間の部分一致性や欠失・挿入だけでなく,配列内の塩基組成分布に関する様々な情報を抽出できることを新たに見出した.さらに,出現頻度符号の配色を工夫することで,DNA配列解析において重要であるGC含有量を出力結果から直感的に把握できることを実験的に確認した.
4.出現頻度符号化法、およびその部分配列長の可変機能を専用端末上に実装し,微視レベルから巨視レベルまでのマルチスケールな配列比較を統合して扱える環境を構築した.この符号化法を用いて,大腸菌K-12株と病原性大腸菌O-157:H7株を比較し,全ゲノム配列から部分一致性を抽出することに成功した.
日本学術振興会, 特定領域研究, 大阪大学, 14015218

高温溶媒系で生きる細胞の戦略
科学研究費助成事業
2001 - 2001
森川 正章
日本学術振興会, 基盤研究(B), 大阪大学, 13450343

空間符号モアレマッチング法による鳥瞰的ゲノム情報解析
科学研究費助成事業
2001 - 2001
谷田 純, 森川 正章
配列情報の空間符号化を利用した配列間マッチング技術に対して,符号化規則の変更や出力モアレ縞の解釈によって得られるゲノム配列間の情報抽出法を検討した.これらの手法を,公開ゲノム配列データベースより取得したDNA及び,アミノ酸配列に適用し,評価を行った.その結果,以下に示す成果を得た.
1.モアレ情報端末の利用性向上のため,液晶ディスプレイ上に2配列の符号化画像を表示する方式を検討し,所望のマッチング結果を得ることに成功した.これより,コンピュータ制御による簡易視覚端末が実現できることを確認した.
2.アミノ酸配列間の類縁性を含めた比較情報を抽出するため,疎水性パラメータを空間符号に導入する手法を考案し,弱相関配列の検出を試みた.既存のホモロジー検索ツール,モチーフ抽出ツールとの比較から,アミノ酸配列比較技術としての有効性を示した.
3.ドットプロット法との比較により,提案手法が有する特長を明らかにした.モアレ現象の特性により適度な情報抽出が行われ,DNA配列に対してもフィルタリング処理なしで有用な情報が抽出できる点,表示に必要なデータ数を減少でき,高速かつ簡便にデータ更新が行える点,検査範囲が等しければ,より高い視認性で出力が得られる点などである.
4.高密度符号化法を開発し,10万要素の同時比較の可能性を示すことができた.いくつかの特定の縦列反復配列部位において観察される特徴的な2次元モアレパターンを明らかにした.また,処理手順を整理し,自己相関相互相関,反転相関の有用性を示した.これらの成果より,2配列間の類似性を鳥瞰的に視覚化する手法の体系化を行い,提案手法のもつ多様な情報抽出の可能性を提示した.
日本学術振興会, 特定領域研究(C), 大阪大学, 13208014

空間符号モアレ法に基づく光学的ゲノム情報処理技術の開発
科学研究費助成事業
2000 - 2000
谷田 純, 森川 正章
光演算技術は,大容量情報に対する並列処理性と,人間の優れたパターン認識能力を誘起する情報可視性をあわせ持ち,それらの有効利用はゲノム情報処理の効率化に寄与しうる.本研究では,その具体的手法として,空間符号モアレ法による2配列間マッチング演算を対象とし,特性,および,有効性を評価した.
空間符号モアレ法では,符号パターンや実現方法により,出力情報の加工が可能である.相補配列の検出能力をもつ対称型符号パターンを用いて,ステムループ構造の抽出が行えることを確認した.次に,濃度反転符号と非反転符号の組合せによる一致信号の先鋭化技術を考案し,従来方式では不可能であった1塩基置換によるミスマッチの検出を実現した.また,3色の符号パターンにより,3配列間マッチング処理を実現した.
一方,検討手法の簡便な利用をめざして,マッチング情報端末を試作した.2台の液晶ディスプレイ画面を半透鏡で合成する方式と,1台の液晶ディスプレイ画面に印刷済透明シートを張り合わせる方式とを実装した.後者の方式は,データ操作の自由度では劣るものの,視認性の点で極めて優れている.
光演算技術に基づく情報システムとして,自由空間光インターコネクションによる配列マッチング専用プロセッサの基本構成を検討した.空間符号を時分割展開した時間符号が処理スループットの点で優れていることを見い出し,予備実験により原理確認を行った.
空間符号モアレ法は,ホモロジー検索の基本演算であるドット・プロット法と等価な信号を出力する.しかし,モアレ縞は,各要素のマッチング結果が継ぎ目なく表されるアナログ画像情報である.その結果,人間のパターン認識能力をより強く誘起しうる可能性をもつ.符号化については,視覚特性を考慮する必要がある.一つの展開方向として,動態視の利用によりマッチング識別能力を向上させる方式が考えられる.
日本学術振興会, 特定領域研究(C), 大阪大学, 12208028

Studies on degradation mechanism of petroleum under anoxic condition.
Grants-in-Aid for Scientific Research
1996 - 1997
MORIKAWA Masaaki
Bacterial strain HD-1, isolated from an oil field, is able to degrade both aliphatic and aromatic hydrocarbons anaerobically. In this project, bacterial degradation mechanisms of petroleum under anoxic condition was studied Utilizing tetradecane (alkane) as a substrate, two kinds of alkene were accumulated as degradation intermediates in HD-1 cells. Finally a main component of the alkenes was identified as 1-dodecene based on analyzes data from FT-IR,1H-NMR,and GC/MS.This result shows that alkane is dehydrogenated by the strain HD-1 under anoxic condition. Although one example was reported in 1992 from a research group in Germany about alkane degradation under anoxic condition, this is the first report on the structural analysis of a degradation intermediate. Moreover, strain HD-1 were found to be able to grow on CO2 as a sole carbon source and produce alkane in the cells. Its production yield was 0.1% in dry cell weight. The metabolic pathway from CO2 to alkane needs to be elucidated in a future.
Recently, a new bacterial strain TK-122 was isolated oil-reservoir tank. Its oil degradation ability is superior to HD-1 under both oxic and anoxic conditions. About 70% of alkanes (from octane to hexadecane) in petroleum, was degraded in 3 days under anoxic condition. Molibdenate (MoO4^<2->) was used as an electron acceptor in the anaerobic degradation of alkane. This result indicates a finding of a new petroleum degradation pathway. According to the analysis on 16S rRNA nucleotide sequence of the strain TK-122, Escherichia or Citrobacter was most probable genus. However, physiological characterization of the strain matched with none of these genera. It is indicated that the strain TK-122 belongs to a new genus.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Osaka University, 08650942

CO_2固定と石油生成に関する微生物学的アプローチ
科学研究費助成事業
1995 - 1995
森川 正章, 今中 忠行
【1】アルカン/アルケン生合成経路の解明
まず、生物学的にCO_2からアルカン/アルケンを合成する経路のなかで最も研究が遅れており、実際反応律速になっている可能性が高いと思われる脂肪酸からアルカン/アルケンへの変換反応について検討した。緑藻類などを用いた研究結果からは脂肪酸から直接アルカン/アルケンを合成しているのではなく、脂肪酸からアルデヒドになった後アルカン/アルケンに変換される可能性が示唆されている。そこでHD-1株が最も多く蓄積していたヘキサデカン(C16)の前駆物質であると予想されるパルミチン酸あるいはヘキサデカナ-ルを使ってアルカン/アルケンの生成が実際に起こるかを調べた。^<14>C-パルミチン酸は市販のものを利用した。^<14>C-ヘキサデカナ-ルは入手不可能であったため^<14>C-パルミチン酸から化学合成した。アルカン/アルケンの生成反応は以下のようにして行った。基質である^<14>C-パルミチン酸あるいは^<14>C-ヘキサデカナ-ルを含むリン酸緩衝液(pH7.0)/1% Triton X-100をナスフラスコ内でArガス通気により脱酸素処理する。同様に脱酸素処理した細胞抽出液を嫌気性ボックス内で添加後密栓する。これを遮光した湯溶中で37℃24時間保温した。反応産物を含む疎水性画分をクロロホルム抽出し、気質のみで保温したコントロールと共にシリカゲル60TLC(ヘキサンおよびヘキサン,ジエチルエーテル,ギ酸)で展開し、脂肪酸あるいはアルデヒド画分(Rf=0.5-0.7)とアルカン/アルケン画分(Rf=0.9以上)を厳密に分けて回収した。液体シンチレーションカウンターによりそれぞれの放射活性を測定した。この結果から、微量ではあるが細胞抽出液を加えた場合にのみアルカン/アルケンの生成が確認できた。さらに脂肪酸にくらべてアルデヒドの方がアルカン/アルケンの生成率が良いことか、細菌においても脂肪酸はアルデヒドを経由してアルカン/アルケンに変換されることが強く示唆された。現在細胞抽出液をカラムクロマトグラフィーなどにより分画して、本酵素活性(アルデヒドデカルボニラーゼ)の精製を目指している。
【2】アルカン/アルケン合成能力の向上
本プロジェクトが実際に地球環境レベルで貢献できるにはHD-1株の生育速度を高めると共にアルカン/アルケンの蓄積量を飛躍的に増大する必要がある。そこで、人為的突然変異処理を繰り返してこの目的に合った変異株を効率良く選別する方法を検討した。突然変異処理法は細菌において最も強力で汎用的なニトロソグアニジン処理を採用した。処理条件は生存率10%〜50%を設定(100mg/1,37℃,30分間)した。アルカン/アルケンの合成能力の高くなった株はプレート上では元株と容易には区別できない。そこで比重の違いを利用した。すなわちアルカン/アルケンを含む疎水性成分の細胞内含量が高いものは他にくらべて軽くなっていると予想した。比重の違いで生体物質を分画するのに最も簡便な方法のひとつが密度勾配遠心分離法である。そこで細胞を分画するための無菌的密度勾配遠心分離の最適条件検討を策定した。遠心分離後、上層から注意深く溶液を10区画(No.1〜No.10)に分けて回収した。これをプレートに広げて単一細胞を単離して最も比重の軽い細胞(No.5)、中程度の細胞(No.7)、最も重い細胞(No.10)を選んでそれぞれの大量培養後、疎水性成分含量を比較した。取得したNo.5は変異剤未処理の約3倍の疎水性物質を蓄積していた。現在ガスクロマトグラフィーにより疎水性成分(アルカン/アルケン含量など)を分析中である。以上の結果から、突然変異処理(ニトロソグアニジンおよび紫外線照射)と密度勾配遠心分離による選別により、アルカン/アルケンの高生産性突然変異株の取得が可能であることが確認できた。今後、この操作を繰り返すことによってHD-1株のアルカン/アルケン生成能力を可能な限り増強させる予定である。
日本学術振興会, 重点領域研究, 大阪大学, 07263236

Characterization of Anaerobic Petroleum Degrading Bacterium strain HD-1.
Grants-in-Aid for Scientific Research
1993 - 1995
IMANAKA Tadayuki, MORIKAWA Masaaki
We isolated a new type of Gram-negative mixotrophic bacterium, Pseudomonas sp. HD-1, which is a facultative anaerobe. Cells were grown on CO_2 as a sole carbon source in the presence of H_2. Anaerobic growth of the strain was enhanced by the addition of aliphatic as well as aromatic hydrocarbons. When cells were grown on n-alkane anaerobically, many oil vacuoes formed in the cells and about 40-50% of the dry cell weight was lipid and oil. The total increase in the hydrophilic components of the cells indicated the anaerobic assimilation of aliphatic hydrocarbon. Electron microscopic observation showed that the cell surface was highly wavy and that the membrane had a multi-layr structure irrespective of the presence of oil.
The strain was demonstrated to degrade tetradecane (C_<14>) under anaerobic condition and one of the major metabolic intermediates was identified as 1-dodecene (C_<12>). This result demonstrates that alkanes are biodegradable without molecular oxygen via a pathway different from beta-oxidation. The strain was found to grow on CO_2 in the presence of tetradecane and absence of H_2 gas and light. These facts strongly indicate that the bacterium is able to utilize alkane as an energy source anaerobically.
Japan Society for the Promotion of Science, Grant-in-Aid for General Scientific Research (B), Osaka University, 05454037

CO_2固定と石油生成に関する微生物学的アプローチ
科学研究費助成事業
1994 - 1994
森川 正章, 今中 忠行
原材料をCO_2ガス、最終産物を石油成分(特にパラフィン系炭化水素)とする一連のプロセス(炭素物質循環系)の構築を前提として油田土壌から分離されたHP-1株代謝経路の解明ならびに菌株を改良することが本研究の目的である。本年度はHP-1株がCO_2から炭化水素を生成できることの確認とその代謝経路の推定、および遺伝子組み換え法により本菌株を改良するための基盤を確立することを目的とした。
先ず、CO_2を単一炭素源とする培地0.5%(NH_4_2SO_4,0.1%KH_2PO_4,0.05%MgCl_2・6H_2O/水道水(pH7.0)にCO_2を含む混合嫌気ガス(CO_2:H_2:N_2=5:5:90)を通気して37℃2週間培養した。増殖した菌体を遠心分離により回収・洗浄したのち凍結乾燥した。クロロホルム抽出によって調製した疎水性画分についてGC分析を行った結果、パラフィンに相当する位置にピークが検出されたため、さらにGC-MSによる質量数の決定ならびに構造の推定を行った。親イオンピークならびにフラグメントイオンピークの特徴から脂肪酸(パルミチン酸、ステアリン酸)以外に質量数278と226のパラフィン(それぞれ、エイコサジエンならびにヘキサデカン)が確認できた。以上の結果、HP-1株はCO_2とH_2から石油主成分であるパラフィンを生産することを確認した。
次に、本菌のCO_2固定能力ならびにパラフィン合成能力を強化するために遺伝子組み換え基盤技術の確立をめざした。汎用プラスミドベクター14種類についてエレクトロポレーション法によりHD-1株の形質転換を試みたところ、グラム陰性細菌用ベクターRSF1010(Sm^r)によりパルス条件10kV/cm 1 mses2回により2.3×10^3/μg DNA の頻度で形質転換可能であることを見いだした。本研究成果によりCO_2固定経路とパラフィン合成経路における律速段階を触媒する酵素遺伝子群を遺伝子工学的に強化できる見通しがついた言える。それぞれの代謝経路についてその機構を解析することが今後の課題である。
日本学術振興会, 重点領域研究, 大阪大学, 06271245

Petroleum degrading and producing bacteria (under low temperature, low oxygen conditions) Bioremediation technology (Encocrine disruptant degrading bacteria) Biosynthetic pathways of biosurfactant (Non-ribosomal peptide synthetase) Biofilm formation (M・・・　　　　　　　　　　　　　　　
Petroleum degrading and producing bacteria (under low temperature, low oxygen conditions)
Bioremediation technology (Encocrine disruptant degrading bacteria)
Biosynthetic pathways of biosurfactant (Non-ribosomal peptide synthetase)
Biofilm formation (Marine bacteria, Bacillus, Hyperthermophiles)
Competitive research funding

植物成長強化剤及びそれを用いた植物栽培方法　　　　　　　　　　　　　　　
Patent right, 鈴木 和歌子, 菅原 雅之, 三輪 京子, 森川 正章, 玉木 秀幸, 牧野 彩花, 鎌形 洋一
特願2014-148493, 22 Jul. 2014
06 Aug. 2015

植物の生長を促進する方法、植物生長促進物質を製造する方法及びこれらに利用されるタンパク質　　　　　　　　　　　　　　　
Patent right, 森川正章, ジョグ ラフル, 菅原雅之, 三輪京子
特願WO2017002929A1, 30 Jun. 2015

土壌汚染浄化方法　　　　　　　　　　　　　　　
Patent right, 長谷川武, 平本弘, 山本英樹, 岡崎修, 森川正章
特願2009-161546, 08 Jul. 2009
特開2011-016060, 27 Jan. 2011
特許第4955039号
23 Mar. 2012

キノン類の製造方法　　　　　　　　　　　　　　　
Patent right, 森川正章, 大胡康, 鈴木良雄
特願2009-179141, 31 Jul. 2009
特開2011-030487, 17 Feb. 2011
特願2009-179141

新規水草根圏微生物　　　　　　　　　　　　　　　
Patent right, 森川正章, 鷲尾健司, 山賀文子
特願2008-99213, 07 Apr. 2008
特開2009-247279, 29 Oct. 2009

Ｓｈｅｗａｎｅｌｌａｓｐ．ＳＩＢ１株由来の新規なアルカリフォスファターゼ　　　　　　　　　　　　　　　
Patent right, 金谷 茂則, 森川 正章, 春木 満
特願2001-172653, 07 Jun. 2001
特開2002-360259, 17 Dec. 2002
特許第3507890号
09 Jan. 2004

アルカン類を分解する新規微生物、アルカン類を分解する方法　　　　　　　　　　　　　　　
Patent right, 森川 正章, 加藤 智久
特願2000-34638, 14 Feb. 2000
特開2001-224360, 21 Aug. 2001
特許第3120114号

核酸の増幅方法およびその試薬　　　　　　　　　　　　　　　
Patent right, 北林 雅夫, 荒川 琢, 井上 浩明, 川上 文清, 川村 良久, 今中 忠行, 高木 昌宏, 森川 正章
特願2000-153459, 31 May 1995
特開2000-354496, 26 Dec. 2000

熱安定性ＤＮＡポリメラ―ゼ　　　　　　　　　　　　　　　
Patent right, 北林 雅夫, 荒川 琢, 井上 浩明, 川上 文清, 川村 良久, 今中 忠行, 高木 昌宏, 森川 正章
特願平11-191896, 31 May 1995
特開2000-23687, 25 Jan. 2000

蔗糖脂肪酸エステル合成酵素を産生する微生物　　　　　　　　　　　　　　　
Patent right, 今中 忠行, 森川 正章, 西畑 隆男, 神野 和人
特願平9-223803, 20 Aug. 1997
特開平11-056349, 02 Mar. 1999

薯糖脂肪酸エステラーゼ　　　　　　　　　　　　　　　
Patent right, 今中忠行, 森川正章, 西畑隆男, 神野和人
特願平9-223940, 20 Aug. 1997
特開平11-056364, 02 Mar. 1999

耐熱性グリセロールキナーゼ及びそれをコードするＤＮＡ　　　　　　　　　　　　　　　
Patent right, 金谷茂則, 森川正章
特願平9-198961, 24 Jul. 1997
特開平11-32773, 09 Feb. 1999

環状リポペプチド化合物の化学合成方法および環状リポペプチド化合物　　　　　　　　　　　　　　　
Patent right, 今中 忠行, 平田 善彦, 森川 正章
特願平9-118523, 08 May 1997
特開平10-306098, 17 Nov. 1998

新規微生物　　　　　　　　　　　　　　　
Patent right, 森川正章, 金 沙
特願平10-261040, 01 Sep. 1998
特開2000-125849
特許第3120114号

核酸の増幅方法およびその試薬　　　　　　　　　　　　　　　
Patent right, 北林 雅夫, 荒川 琢, 井上 浩明, 川上 文清, 川村 良久, 今中 忠行, 高木 昌宏, 森川 正章
特願平7-134096, 31 May 1995
特開平8-322597, 10 Dec. 1996

超好熱始原菌由来のＤＮＡポリメラーゼ遺伝子およびその用途　　　　　　　　　　　　　　　
Patent right, 今中 忠行, 高木 昌宏, 森川 正章, 柿原 博文
特願平6-95109, 09 May 1994
特開平7-298879, 14 Nov. 1995

シュードモナス属細菌を用いた炭化水素の生産方法及び新規シュードモナス属細菌　　　　　　　　　　　　　　　
Patent right, 今中 忠行, 森川 正章, 桜井 尚二
特願平5-352901, 29 Dec. 1993
01 Aug. 1995

新規微生物　　　　　　　　　　　　　　　
Patent right, 森川 正章
特願平4-109354, 02 Apr. 1992
特開平5-276933, 26 Oct. 1993

バイオサーファクタントの濃縮方法　　　　　　　　　　　　　　　
Patent right, 桜井 尚二, 今中 忠行, 森川 正章
特願平3-182022, 26 Jun. 1991
特開平5-211876, 24 Aug. 1993

バイオサーファクタント産生菌のスクリーニング方法及びバイオサーファクタントの活性測定方法　　　　　　　　　　　　　　　
Patent right, 桜井 尚二, 真鍋 洋平, 今中 忠行, 森川 正章
特願平3-181991, 26 Jun. 1991
特開平5-211892, 24 Aug. 1993

改変ヒト・リゾチーム、それをコードする遺伝子及び改変ヒト・リゾチームの製造方法　　　　　　　　　　　　　　　
Patent right, 村木三智郎, 地神芳文, 田中秀明, 森川正章
特願昭62-278060, 02 Nov. 1987
特開平1-120286, 12 May 1989

植物の生長を促進する方法、植物生長促進物質を製造する方法及びこれらに利用されるタンパク質　　　　　　　　　　　　　　　
Patent right, 森川 正章
特願2015-130895

卵黄レシチンの製造方法　　　　　　　　　　　　　　　
Patent right, 森川正章, 徳永恵美子, 堺宗雄
特願昭61-104787
特開昭62-263192