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Search DetailsAraki Hitoshi
| Research Faculty of Agriculture Fundamental AgriScience Research Agrobiology and Bioresources | Professor |
| One Health Research Center | Professor |
Researcher basic information
■ Degree■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research KeywordResearch Field
- Life Science, Biodiversity and systematics
- Life Science, Ecology and environment
- Life Science, Evolutionary biology
- Bachelor's degree program, School of Agriculture
- Master's degree program, Graduate School of Agriculture
- Doctoral (PhD) degree program, Graduate School of Agriculture
Career
■ CareerCareer
- Apr. 2024 - Present
Kyushu University, Center for Research and Education of Environmental Technology Faculty of Engineering, Visiting professor - Apr. 2013 - Present
Hokkaido University, Research Faculty of Agriculture, Devision of Environmental Resources, Professor - Sep. 2023 - Dec. 2023
McGill University, Department of Biology, Faculty of Science, Visiting professor, Canada - Jan. 2008 - Mar. 2013
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Fish Ecology and Evolution, Group leader - Jul. 2004 - Dec. 2007
Oregon State University, Department of Zoology, Postdoctoral research associate - Apr. 2001 - Jun. 2004
University of Chicago, Department of Ecology and Evolution, Postdoctoral fellow
- Apr. 1997 - Mar. 2001, Kyushu University, Graduate School of Medical Science
- Apr. 1995 - Mar. 1997, Kyushu University, Graduate School of Science
- Apr. 1991 - Mar. 1995, Kyushu University, Faculty of Science, Department of Biology
Research activity information
■ Awards- 2007, Society of Evolutionary Studies, Japan, Awards for encouragement of young scientist
Hitoshi Araki
- Large-scale environmental DNA survey reveals niche axes of a regional coastal fish community
Yutaka Osada; Masaki Miya; Hitoshi Araki; Hideyuki Doi; Akihide Kasai; Reiji Masuda; Toshifumi Minamoto; Satoquo Seino; Teruhiko Takahara; Satoshi Yamamoto; Hiroki Yamanaka; Mitsuhiro Aizu-Hirano; Keiichi Fukaya; Takehiko Fukuchi; Ryo O. Gotoh; Masakazu Hori; Midori Iida; Tomohito Imaizumi; Tadashi Kajita; Takashi Kanbe; Tanaka Kenta; Yumi Kobayashi; Tomohiko Matsuura; Hiroki Mizumoto; Hiroyuki Motomura; Hiroaki Murakami; Kenji Nohara; Shin-ichiro Oka; Tetsuya Sado; Hiroshi Senou; Koichi Shibukawa; Tomoki Sunobe; Hiroshi Takahashi; Koji Takayama; Katsuhiko Tanaka; Hisashi Yamakawa; Satoru Yokoyama; Seokjin Yoon; Michio Kondoh
Scientific Reports, 16, 1, Springer Science and Business Media LLC, 16 Feb. 2026
Scientific journal, Abstract
The concept of the ecological niche, defined as the basic habitat requirements for a species, is central to understanding species geographic distributions and predicting their responses to environmental change. However, identifying the essential niche for large regional communities remains a challenge because niche axes can be “hidden” by the complexity of the underlying ecological processes. Here, applying advanced species distribution modelling to nationwide environmental DNA survey data, we identified hidden niche axes of the Japanese coastal fish community and investigated the response diversity to these axes. Our survey detected 1,220 coastal fish species. The hidden niche axes collectively explained most of the variation in fish biodiversity and revealed five biogeographic boundaries for the regional community. These niches of the Japanese fish community may primarily relate to several processes due to ocean currents, such as environmental filters, transport from source areas and dispersal barriers. We also found that the response diversity to niche axes was positively correlated with species richness, although local communities with particularly high response diversity were geographically biased. A better understanding of the niche axes of the regional ecological community should help to mitigate the loss of biodiversity and ecosystem services caused by ongoing environmental change. - High-frequency eDNA metabarcoding survey data of coastal fish across the Japanese Archipelago
Hideyuki Doi; Masaki Miya; Hitoshi Araki; Akihide Kasai; Reiji Masuda; Toshifumi Minamoto; Satoquo Seino; Hiroki Yamanaka; Tetsuya Sado; Hisashi Yamakawa; Hyojin Ahn; Mitsuhiro Aizu-Hirano; Seiji Arakaki; Ryutaro Goto; Keisuke Koizumi; Nobuchika Hara; Yasuhisa Henmi; Masakazu Hori; Midori Iida; Tadashi Kajita; Yumi Kobayashi; Manabu Kume; Shin-ichiro Oka; Shunsuke Matsuoka; Yuki Minegishi; Mariko Nagano; Teruhiko Takahara; Hiroshi Takahashi; Sayaka Takahashi; Koji Takayama; Kunihiro Yamauchi; Michio Kondoh
EcoEvoRxiv, California Digital Library (CDL), 17 Jun. 2025
English, Scientific journal, The Japanese archipelago, spanning over 3,000 km from subarctic to subtropical zones (approximately 24°N to 45°N), hosts a diverse array of coastal fish communities shaped by pronounced latitudinal gradients. Here, we present a high-frequency environmental DNA (eDNA) metabarcoding dataset of coastal fishes collected across the archipelago. Using the MiFish primer set targeting a hypervariable region of the mitochondrial 12S rRNA gene, we conducted surveys at 195 sites, yielding 690 samples collected through one-time (annual), monthly, and biweekly sampling schemes. The inclusion of high-frequency sampling enabled the detection of fine-scale temporal variations in fish community composition. From January 2018 to March 2019, eDNA samples were collected along with metadata on geographic and environmental variables, including coastal structure, wave height, tide, currents, and water temperature. The resulting dataset comprises fish eDNA records for 1,477 species across 171 families. This dataset offers a valuable resource for investigating fish community structure, temporal dynamics, and biodiversity in the coastal ecosystems of the Japanese archipelago, supporting a range of applications in ecology, conservation, and eDNA-based research. - Exploring the Dynamics of Environmental DNA: Effects of Early Developmental Stage and Physiological State in Chum Salmon
Masayuki K. Sakata; Takashi Kanbe; Shunpei Sato; Hitoshi Araki
Environmental DNA, 7, 3, Wiley, Jun. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, ABSTRACT
Environmental DNA (eDNA) analyses provide valuable ecological data. Recent studies have explored eDNA dynamics related to reproductive behavior and developmental stages, revealing significant variations in eDNA concentrations across different life stages. However, there is a gap in understanding the association between eDNA concentrations and changes before and after developmental events, such as egg hatching. This study addresses this gap by monitoring eDNA signals in chum salmon (Oncorhynchus keta) during their early developmental stages and examining the effects of their physiological and behavioral changes. For this purpose, eDNA flux was monitored in rearing experiments with chum salmon during their developmental stages (egg, alevin, and fry). The eDNA flux varied significantly across different developmental stages: while no eDNA was detected during the egg stage, eDNA flux increased rapidly after hatching. After hatching, the eDNA flux became stable during the alevin stage but increased approximately 30‐fold when they progressed into the fry stage (LMM and post hoc Tukey‐HSD test: p < 0.05). These results suggest that eDNA signals vary across the developmental stages and can be utilized to estimate and monitor fish development even under natural conditions, such as those occurring under gravel for salmonid species. - Spatial heterogeneity of eDNA concentration as a predictor of small biomass of fish in a mountain stream
Hirokazu Urabe; Hiroki Mizumoto; Fumi Tsuda-Yamaguchi; Hitoshi Araki
Limnology, 26, 1, 223, 233, Springer Science and Business Media LLC, 04 Dec. 2024
Scientific journal - Human activity-associated establishment of invasive mink population estimated using environmental DNA
Toshihiro Takaba; Masayuki K. Sakata; Takashi Kanbe; Takashi Mitsuzuka; Shouko Inoue; Hiroki Mizumoto; Takahiro Nobetsu; Hitoshi Araki
Biological Invasions, Springer Science and Business Media LLC, 31 Jul. 2024
Scientific journal - A link between evolution and society fostering the UN sustainable development goals.
Luc De Meester; Ella Vázquez-Domínguez; Rees Kassen; Félix Forest; Mauricio R Bellon; Britt Koskella; Rosa A Scherson; Licia Colli; Andrew P Hendry; Keith A Crandall; Daniel P Faith; Craig J Starger; R Geeta; Hitoshi Araki; Ehsan M Dulloo; Caroline Souffreau; Sibylle Schroer; Marc T J Johnson
Evolutionary applications, 17, 6, e13728, Jun. 2024, [International Magazine]
English, Scientific journal, Given the multitude of challenges Earth is facing, sustainability science is of key importance to our continued existence. Evolution is the fundamental biological process underlying the origin of all biodiversity. This phylogenetic diversity fosters the resilience of ecosystems to environmental change, and provides numerous resources to society, and options for the future. Genetic diversity within species is also key to the ability of populations to evolve and adapt to environmental change. Yet, the value of evolutionary processes and the consequences of their impairment have not generally been considered in sustainability research. We argue that biological evolution is important for sustainability and that the concepts, theory, data, and methodological approaches used in evolutionary biology can, in crucial ways, contribute to achieving the UN Sustainable Development Goals (SDGs). We discuss how evolutionary principles are relevant to understanding, maintaining, and improving Nature Contributions to People (NCP) and how they contribute to the SDGs. We highlight specific applications of evolution, evolutionary theory, and evolutionary biology's diverse toolbox, grouped into four major routes through which evolution and evolutionary insights can impact sustainability. We argue that information on both within-species evolutionary potential and among-species phylogenetic diversity is necessary to predict population, community, and ecosystem responses to global change and to make informed decisions on sustainable production, health, and well-being. We provide examples of how evolutionary insights and the tools developed by evolutionary biology can not only inspire and enhance progress on the trajectory to sustainability, but also highlight some obstacles that hitherto seem to have impeded an efficient uptake of evolutionary insights in sustainability research and actions to sustain SDGs. We call for enhanced collaboration between sustainability science and evolutionary biology to understand how integrating these disciplines can help achieve the sustainable future envisioned by the UN SDGs. - Environmental DNA unveils deep phylogeographic structure of a freshwater fish
Tetsu Yatsuyanagi; Takashi Kanbe; Kazuya Fujii; Shouko Inoue; Hitoshi Araki
Molecular Ecology, 33, 9, e17337, Wiley, Apr. 2024, [International Magazine]
English, Scientific journal, Abstract
Phylogeography bears an important part in ecology and evolution. However, current phylogeographic studies are largely constrained by limited numbers of individual samples. Using an environmental DNA (eDNA) assay for phylogeographic analyses, this study provides detailed information regarding the history of Siberian stone loach Barbatula toni, a primary freshwater fish across the whole range of Hokkaido, Japan. Based on an eDNA metabarcoding on 293 river water samples, we detected eDNA from B. toni in 189 rivers. A total of 51 samples, representing the entire island, were then selected from the B. toni eDNA‐positive sample set for the subsequent analyses. To elucidate the phylogeographic structure of B. toni, newly developed eDNA metabarcoding primers (Barba‐cytb‐F/R) were applied to these samples, specifically targeting their haplotypic variation in cytochrome b. After a bioinformatic processing to mitigate haplotypic false positives, a total of 50 eDNA haplotypes were identified. Two regionally restricted, genetically distinct lineages of the species were revealed as a result of phylogeographic analyses on the haplotypes and tissue‐derived DNA from B. toni. According to a molecular clock analysis, they have been genetically isolated for at least 1.5 million years, suggesting their ancient origin and colonisation of Hokkaido, presumably in the glacial periods. These results demonstrate how freshwater fishes can alter their distributions over evolutionary timescales and how eDNA assay can deepen our understanding of phylogeography. - Co-occurrence patterns of endangered Sakhalin taimen and introduced rainbow trout in Hokkaido, Japan, inferred by environmental DNA metabarcoding
Takashi Kanbe; Hiroki Mizumoto; Takashi Mitsuzuka; Nobuyoshi Nakajima; Hitoshi Araki
Aquatic Conservation: Marine and Freshwater Ecosystems, 33, 12, 1492, 1500, Dec. 2023
Scientific journal - The eDNA Society International Meeting 2023, “moving from knowledge into practice”
Hiroki Yamanaka; Hideyuki Doi; Hitoshi Araki; Kimiko Uchii; Toshifumi Minamoto
Environmental DNA, Wiley, 14 Aug. 2023 - Species traits and ecosystem characteristics affect species detection by eDNA metabarcoding in lake fish communities
Hideyuki Doi; Shunsuke Matsuoka; Shin ichiro S. Matsuzaki; Mariko Nagano; Hirotoshi Sato; Hiroki Yamanaka; Saeko Matsuhashi; Satoshi Yamamoto; Toshifumi Minamoto; Hitoshi Araki; Kousuke Ikeda; Atsuko Kato; Kouichi Kumei; Nobutaka Maki; Takashi Mitsuzuka; Teruhiko Takahara; Kimihito Toki; Natsuki Ueda; Takeshi Watanabe; Kanji Yamazoe; Masaki Miya
Freshwater Biology, 68, 8, 1346, 1358, Wiley, Aug. 2023, [Peer-reviewed]
English, Scientific journal - Functional eradication of largemouth bass in Kanabara Dam Lake, Japan
Tsuboi Jun-ichi; Katano Osamu; Mizumoto Hiroki; Araki Hitoshi
Japanese Journal of Conservation Ecology, 28, 2, 437, 452, The Ecological Society of Japan, 2023
Japanese, Largemouth bass (Micropterus nigricans) is listed among the world’s 100 worst invasive alien species by the International Union for Conservation of Nature (IUCN). Because of M. nigricans’ serious negative impacts on aquatic ecosystems that it invades, major efforts have been made to control and locally eradicate it. However, there are few reports of its complete eradication from lake or river systems. Here, we discuss a comprehensive program of M. nigricans eradication in Kanabara Dam Lake, Nagano Prefecture, Japan. Operating since 2007, the program includes the removal of spawning beds; removal of larvae and fry through scooping; and capture of immature and adult fish via net traps, cast nets, gill nets, fishing, and spear guns, and by hand. The M. nigricans population was monitored via snorkelling and environmental DNA analysis. Net traps were effective for capturing smaller fish, whereas 3-meter-long gill nets and spear guns were effective for larger fish. Yearly catches of M. nigricans reached a maximum of 1,472 in 2010, excluding young-of-the-year. The number of spawning beds reached a maximum of 131 in 2012 but decreased rapidly and substantially thereafter. In 2014 > 5,000 larvae were observed around a few spawning beds, having been missed at the time of spawning. Most of these larvae were quickly caught via hand nets from the shore or by snorkelling. Interestingly, Amur goby (orange type, Rhinogobius kurodai) increased in abundance during the eradication program and preyed upon M. nigricans eggs. We found no evidence of new M. nigricans spawning beds after 2015, and the last adult was captured in 2018. These results suggest that no breeding of largemouth bass occurred after 2018 and point to functional eradication with no further population growth in the lake. On the other hand, our environmental DNA analysis detected M. nigricans-specific DNA in all four surveys conducted between 2018 and 2022. Therefore, largemouth bass may still persist in or around Kanabara Dam Lake, although the estimated DNA concentrations were very low and we found no sign of increase after 2018. This suggests that, despite the apparent success of eradication efforts, the risk of reintroduction remains and ongoing monitoring is needed. - Application of eDNA methods to evaluate abundance and reproduction of winter-breeding freshwater mussels (Buldowskia iwakawai) in the Ishikari River floodplain
J. Wu; J. N. Negishi; H. Izumi; T. Kanbe; H. Mizumoto; H. Araki
Hydrobiologia, 2023
Scientific journal - edna2021: The fourth annual meeting of The eDNA Society “Species distributions, and beyond”
Hitoshi Araki; Takashi Kanbe; Hiroki Yamanaka; Yuki Minegishi; Noriyuki Koizumi
Environmental DNA, 4, 3, 487, 491, May 2022, [Peer-reviewed], [Lead author] - Utilizing environmental DNA for wide-range distributions of reproductive area of an invasive terrestrial toad in Ishikari river basin in Japan
Hiroki Mizumoto; Osamu Kishida; Kotaro Takai; Naru Matsuura; Hitoshi Araki
Biological Invasions, 24, 4, 1199, 1211, Apr. 2022
English, Scientific journal - Hokkaido-wide eDNA Monitoring for Sakhalin Taimen, Endangered Salmonid Species
Hiroki Mizumoto; Hitoshi Araki
Technical Report, 17, North Pacific Anadromous Fish Commission, 29 Nov. 2021
Scientific journal - Time to adjust to a new feeding environment determines survival and growth of wild-caught white-spotted charr (Salvelinus leucomaenis pluvius)
Kouta Miyamoto; Miloš Đorđević; Hitoshi Araki
Environmental Biology of Fishes, 104, 8, 959, 966, Aug. 2021, [Peer-reviewed], [Last author], [Internationally co-authored], [International Magazine]
Scientific journal - Effectiveness of check-dam improvement on freshwater fishes: assessment based on long-term monitoring and environmental DNA applicability.
HAYAMIZU Masato; ISHIYAMA Nobuo; MIZUMOTO Hiroki; KANBE Takashi; SHIMODA Kazutaka; MISAKA Naoyuki; URABE Hirokazu; NAGASAKA Akiko; NAGASAKA Yu; ONO Satoru; ARAKI Hitoshi; NAKAJIMA Nobuyoshi; FUKUSHIMA Michio
Ecology and Civil Engineering, 24, 1, 61, 73, Ecology and Civil Engineering Society, Jul. 2021, [Peer-reviewed], [Domestic magazines]
Japanese, Scientific journal, 河川上流域に多数設置された治山ダムは,渓流や森林の荒廃を防ぐ重要な役割を果たす一方で,渓流魚の河川内の自由な移動を阻害している.こうした状況に鑑み,河川生態系の保全を目的とした改良事業(魚道の設置,堤体の切り下げ)が国内の多くの治山ダムで実施されている.本研究では,北海道 3 河川に設置された治山ダムを対象に,魚道設置と切り下げによるダム改良工事が,その上下流の渓流魚類相に与える影響について,改良工事前後で行われた採捕調査によって検証した.同時に,ダム改良後の採捕結果と環境 DNA メタバーコーディングによる魚類相推定結果を比較し,治山ダム改良事業における環境 DNA メタバーコーディングを用いた魚類相推定の有効性を検討した.採捕調査の結果,治山ダムの改良事業によって,遡河回遊魚であるアメマスとサクラマスのダム上流への移動を可能にし,10 年後においても効果が確認された.環境 DNA メタバーコーディングでは,採捕された全 9 種に加え,採捕では確認されなか ったニジマスの計 10 種が検出され,治山ダム上下流に設定した各調査地点における遡河回遊魚のアメマス・サクラマスや,ハナカジカの採捕結果との一致率は 70-90 %であった.環境 DNA メタバーコーディングを治山ダム改良事業に適用する場合,評価対象魚種の特性や過去の採捕記録を考慮する必要はあるものの,改良前の治山ダムにおける魚類の遡上阻害の推定や,改良後の河川の魚類相・生息状況の推定に有効であることが示唆された.但し,治山ダムの切り下げを例にとると,遡上阻害の改善効果が維持される期間は工法によって異なる可能性もあり,正確な評価には様々な河川・工法を対象としたさらなる研究の蓄積が必要である. - Artificially increased habitat complexity reduces predation-induced mortality for juvenile salmon in simplified environments
Kouta Miyamoto; Miloš Đorđević; Hitoshi Araki
Environmental Biology of Fishes, 104, 2, 203, 209, Feb. 2021, [Peer-reviewed], [Last author], [Internationally co-authored], [International Magazine]
Scientific journal - Effectiveness of check-dam improvement on freshwater fishes: Assessment based on long-term monitoring and environmental DNA applicability
Masato Hayamizu; Nobuo Ishiyama; Hiroki Mizumoto; Takashi Kanbe; Kazutaka Shimoda; Naoyuki Misaka; Hirokazu Urabe; Akiko Nagasaka; Yu Nagasaka; Satoru Ono; Hitoshi Araki; Nobuyoshi Nakajima; Michio Fukushima
Ecology and Civil Engineering, 24, 1, 61, 73, 2021
Scientific journal - An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols
Toshifumi Minamoto; Masaki Miya; Tetsuya Sado; Satoquo Seino; Hideyuki Doi; Michio Kondoh; Keigo Nakamura; Teruhiko Takahara; Satoshi Yamamoto; Hiroki Yamanaka; Hitoshi Araki; Wataru Iwasaki; Akihide Kasai; Reiji Masuda; Kimiko Uchii
Environmental DNA, 3, 1, 8, 13, Wiley, Jan. 2021, [Peer-reviewed], [International Magazine] - An Environmental DNA Survey on Distribution of an Endangered Salmonid Species, Parahucho perryi, in Hokkaido, Japan
Hiroki Mizumoto; Takashi Mitsuzuka; Hitoshi Araki
Frontiers in Ecology and Evolution, 8, 06 Nov. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal - Understanding seasonal migration of Shishamo smelt in coastal regions using environmental DNA
Tetsu Yatsuyanagi; Hitoshi Araki
PLoS ONE, 15, 10 October, e0239912, Oct. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal - Environmental DNA monitoring for short-term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus)
Tetsu Yatsuyanagi; Ryotaro Ishida; Masayuki K. Sakata; Takashi Kanbe; Hiroki Mizumoto; Yumi Kobayashi; Shoko Kamada; Satoko Namba; Hisaya Nii; Toshifumi Minamoto; Hitoshi Araki
Environmental DNA, 2, 2, 130, 139, Wiley, Apr. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal - When is it good to be shy? Experimental evaluation of predation of juvenile salmon by riparian wildlife
Kouta Miyamoto; Hitoshi Araki
Hydrobiologia, 847, 3, 713, 725, 01 Feb. 2020, [Peer-reviewed], [Last author], [International Magazine]
English, Scientific journal - Is there sampling bias in biological photos with location information collected in a citizen participatory research?-Based on a comparison of citizen and expert data of anadromous salmon and related photographs-
佐橋玄記; 丸山緑; 有賀望; 森田健太郎; 岡本康寿; 向井徹; 水本寛基; 植田和俊; 藤井和也; 渡辺恵三; 大熊一正; 荒木仁志; 佐橋玄記; 丸山緑; 有賀望; 森田健太郎; 岡本康寿; 向井徹; 水本寛基; 植田和俊; 藤井和也; 渡辺恵三; 大熊一正; 荒木仁志
保全生態学研究, 25, 2, 2020, [Peer-reviewed], [Domestic magazines] - Evaluation of an Environmental DNA Method as a Potential Tool for Monitoring Salmonid Fishes in the Wild
Hitoshi Araki; Hiroki Mizumoto; Takashi Kanbe; Shunpei Sato
Technical Report, 15, 164, 167, North Pacific Anadromous Fish Commission, Dec. 2019
Scientific journal - Effects of water depth and structural complexity on survival and settlement of white-spotted charr (Salvelinus leucomaenis)
Kouta Miyamoto; Hitoshi Araki
Hydrobiologia, 840, 1, 103, 112, 01 Sep. 2019, [Peer-reviewed], [Last author], [Domestic magazines]
English, Scientific journal - Ongoing localized extinctions of stream-dwelling white-spotted charr populations in small dammed-off habitats of Hokkaido Island, Japan
Kentaro Morita; Genki Sahashi; Masaki Miya; Shouko Kamada; Takashi Kanbe; Hitoshi Araki
Hydrobiologia, 840, 1, 207, 213, 01 Sep. 2019, [Peer-reviewed], [Last author], [International Magazine]
English, Scientific journal - Spatiotemporal distribution of juvenile chum salmon in Otsuchi Bay, Iwate, Japan, inferred from environmental DNA
Yuki Minegishi; Marty Kwok Shing Wong; Takashi Kanbe; Hitoshi Araki; Tomomi Kashiwabara; Minoru Ijichi; Kazuhiro Kogure; Susumu Hyodo
PLoS ONE, 14, 9, e0222052, 01 Sep. 2019, [Peer-reviewed], [Internationally co-authored], [International Magazine]
English, Scientific journal - Sustainable conservation management and research issues for Japanese chum salmon under the changing climate
帰山雅秀; 荒木仁志; 宮下和士; 永田光博; 佐々木義隆; 浦和茂彦
海洋と生物, 40, 5, 459, 466, Oct. 2018
Japanese - 環境変動下におけるサケの持続可能な資源管理:国際サーモン年に向けたプロローグ
浦和茂彦; 荒木仁志; 宮下和士; 永田光博; 佐々木義隆; 帰山雅秀
海洋と生物, 40, 4, 315, 318, 生物研究社, Aug. 2018
Japanese, Scientific journal - Modeling fitness changes in wild Atlantic salmon populations faced by spawning intrusion of domesticated escapees
Marco Castellani; Mikko Heino; John Gilbey; Hitoshi Araki; Terje Svåsand; Kevin A. Glover
Evolutionary Applications, 11, 6, 1010, 1025, Jul. 2018, [Peer-reviewed], [Internationally co-authored], [International Magazine]
English, Scientific journal - Establishing an environmental DNA method to detect and estimate the biomass of Sakhalin taimen, a critically endangered Asian salmonid
Hiroki Mizumoto; Hirokazu Urabe; Takashi Kanbe; Michio Fukushima; Hitoshi Araki
Limnology, 19, 2, 219, 227, Apr. 2018, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal - Experimental evaluation of predation of stocked salmon by riparian wildlife: effects of prey size and predator behaviours
Kouta Miyamoto; Theodore E. Squires; Hitoshi Araki
MARINE AND FRESHWATER RESEARCH, 69, 3, 446, 454, 2018, [Peer-reviewed], [Last author], [Internationally co-authored], [International Magazine]
English, Scientific journal - Sustainable management of chum salmon in changing environments
Shigehiko Urawa; Hitoshi Araki; Kazushi Miyashita; Mitsuhiro Nagata; Yoshitaka Sasaki; Masahide Kaeriyama
Nippon Suisan Gakkaishi (Japanese Edition), 84, 5, 921, 921, 2018, [Peer-reviewed]
Japanese, Scientific journal - Environmental DNA for salmonid studies
Hitoshi Araki; Takashi Kanbe; Hiroki Mizumoto; Shoko Kamada; Shunpei Sato
Nippon Suisan Gakkaishi (Japanese Edition), 84, 5, 936, 936, 2018, [Peer-reviewed]
Japanese - Half a century of genetic interaction between farmed and wild Atlantic salmon: Status of knowledge and unanswered questions
Kevin A. Glover; Monica F. Solberg; Phil McGinnity; Kjetil Hindar; Eric Verspoor; Mark W. Coulson; Michael M. Hansen; Hitoshi Araki; Oystein Skaala; Terje Svasand
FISH AND FISHERIES, 18, 5, 890, 927, Sep. 2017, [Peer-reviewed]
English, Scientific journal - Differentiated predation risk on hatchery-reared juvenile masu salmon by white-spotted charr with different body sizes
Kouta Miyamoto; Hitoshi Araki
FISHERIES SCIENCE, 83, 2, 245, 250, Mar. 2017, [Peer-reviewed]
English, Scientific journal - Understanding and monitoring the consequences of human impacts on intraspecific variation
Makiko Mimura; Tetsukazu Yahara; Daniel P. Faith; Ella Vazquez-Dominguez; Robert I. Colautti; Hitoshi Araki; Firouzeh Javadi; Juan Nunez-Farfan; Akira S. Mori; Shiliang Zhou; Peter M. Hollingsworth; Linda E. Neaves; Yuya Fukano; Gideon F. Smith; Yo-Ichiro Sato; Hidenori Tachida; Andrew P. Hendry
EVOLUTIONARY APPLICATIONS, 10, 2, 121, 139, Feb. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal - The eco-evolutionary impacts of domestication and agricultural practices on wild species
Martin M. Turcotte; Hitoshi Araki; Daniel S. Karp; Katja Poveda; Susan R. Whitehead
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 372, 1712, Jan. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal - Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea
Satoshi Yamamoto; Reiji Masuda; Yukuto Sato; Tetsuya Sado; Hitoshi Araki; Michio Kondoh; Toshifumi Minamoto; Masaki Miya
SCIENTIFIC REPORTS, 7, 40368, 40368, Jan. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal - Environmental DNA as a 'Snapshot' of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan (vol 11, e0149786, 2016)
Satoshi Yamamoto; Kenji Minami; Keiichi Fukaya; Kohji Takahashi; Hideki Sawada; Hiroaki Murakami; Satsuki Tsuji; Hiroki Hashizume; Shou Kubonaga; Tomoya Horiuchi; Masamichi Hongo; Jo Nishida; Yuta Okugawa; Ayaka Fujiwara; Miho Fukuda; Shunsuke Hidaka; Keita W. Suzuki; Masaki Miya; Hitoshi Araki; Hiroki Yamanaka; Atsushi Maruyama; Kazushi Miyashita; Reiji Masuda; Toshifumi Minamoto; Michio Kondoh
PLOS ONE, 11, 4, e0153291, Apr. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal - Environmental DNA as a 'Snapshot' of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan
Satoshi Yamamoto; Kenji Minami; Keiichi Fukaya; Kohji Takahashi; Hideki Sawada; Hiroaki Murakami; Satsuki Tsuji; Hiroki Hashizume; Shou Kubonaga; Tomoya Horiuchi; Masamichi Hongo; Jo Nishida; Yuta Okugawa; Ayaka Fujiwara; Miho Fukuda; Shunsuke Hidaka; Keita W. Suzuki; Masaki Miya; Hitoshi Araki; Hiroki Yamanaka; Atsushi Maruyama; Kazushi Miyashita; Reiji Masuda; Toshifumi Minamoto; Michio Kondoh
PLOS ONE, 11, 3, e0149786, Mar. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal - An Individual-Based Diploid Model Predicts Limited Conditions Under Which Stochastic Gene Expression Becomes Advantageous
Tomotaka Matsumoto; Katsuhiko Mineta; Naoki Osada; Hitoshi Araki
FRONTIERS IN GENETICS, 6, NOV, 336, 336, Nov. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal - IBSEM: An Individual-Based Atlantic Salmon Population Model
Marco Castellani; Mikko Heino; John Gilbey; Hitoshi Araki; Terje Svasand; Kevin A. Glover
PLOS ONE, 10, 9, e0138444, Sep. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal - MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
M. Miya; Y. Sato; T. Fukunaga; T. Sado; J. Y. Poulsen; K. Sato; T. Minamoto; S. Yamamoto; H. Yamanaka; H. Araki; M. Kondoh; W. Iwasaki
ROYAL SOCIETY OPEN SCIENCE, 2, 7, 150088, Jul. 2015, [Peer-reviewed]
English, Scientific journal - MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
M. Miya; Y. Sato; T. Fukunaga; T. Sado; J. Y. Poulsen; K. Sato; T. Minamoto; S. Yamamoto; H. Yamanaka; H. Araki; M. Kondoh; W. Iwasaki
ROYAL SOCIETY OPEN SCIENCE, 2, 7, Jul. 2015, [Peer-reviewed]
English, Scientific journal - Population genetics of non-genetic traits: Evolutionary roles of stochasticity in gene expression
Katsuhiko Mineta; Tomotaka Matsumoto; Naoki Osada; Hitoshi Araki
GENE, 562, 1, 16, 21, May 2015, [Peer-reviewed], [International Magazine]
English - Genome-Wide Survey of Pseudogenes in 80 Fully Resequenced Arabidopsis thaliana Accessions
Long Wang; Weina Si; Yongfang Yao; Dacheng Tian; Hitoshi Araki; Sihai Yang
PLOS ONE, 7, 12, e51769, Dec. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal - Stocking of captive-bred fish can cause long-term population decline and gene pool replacement: predictions from a population dynamics model incorporating density-dependent mortality
Akiko Satake; Hitoshi Araki
THEORETICAL ECOLOGY, 5, 2, 283, 296, May 2012, [Peer-reviewed]
English, Scientific journal - Spatial and temporal variation in population genetic structure of wild Nile tilapia (Oreochromis niloticus) across Africa
Etienne Bezault; Patricia Balaresque; Aboubacar Toguyeni; Yves Fermon; Hitoshi Araki; Jean-Francois Baroiller; Xavier Rognon
BMC GENETICS, 12, 102, 102, Dec. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal - Avoidance of Long Mononucleotide Repeats in Codon Pair Usage
Tingting Gu; Shengjun Tan; Xiaoxi Gou; Hitoshi Araki; Dacheng Tian
GENETICS, 186, 3, 1077, U490, Nov. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal - Genome-wide investigation reveals high evolutionary rates in annual model plants
Jia-Xing Yue; Jinpeng Li; Dan Wang; Hitoshi Araki; Dacheng Tian; Sihai Yang
BMC PLANT BIOLOGY, 10, 242, 242, Nov. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal - No evidence for large differences in genomic methylation between wild and hatchery steelhead (Oncorhynchus mykiss)
Michael S. Blouin; Virginie Thuillier; Becky Cooper; Vindhya Amarasinghe; Laura Cluzel; Hitoshi Araki; Christoph Grunau
CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 67, 2, 217, 224, Feb. 2010, [Peer-reviewed]
English, Scientific journal - Multilocus analysis of genetic divergence between outcrossing Arabidopsis species: evidence of genome-wide admixture
Wei-Kuang Wang; Chuan-Wen Ho; Kuo-Hsiang Hung; Kuo-Hsiung Wang; Chi-Chun Huang; Hitoshi Araki; Chi-Chuan Hwang; Tsai-Wen Hsu; Naoki Osada; Tzen-Yuh Chiang
NEW PHYTOLOGIST, 188, 2, 488, 500, 2010, [Peer-reviewed]
English, Scientific journal - Is hatchery stocking a help or harm? Evidence, limitations and future directions in ecological and genetic surveys
Hitoshi Araki; Corinne Schmid
AQUACULTURE, 308, SUPPL.1, S2, S11, 2010, [Peer-reviewed]
English - Carry-over effect of captive breeding reduces reproductive fitness of wild-born descendants in the wild
Hitoshi Araki; Becky Cooper; Michael S. Blouin
BIOLOGY LETTERS, 5, 5, 621, 624, Oct. 2009, [Peer-reviewed]
English, Scientific journal - Genomewide Association between Insertions/Deletions and the Nucleotide Diversity in Bacteria
Liucun Zhu; Qiang Wang; Ping Tang; Hitoshi Araki; Dacheng Tian
MOLECULAR BIOLOGY AND EVOLUTION, 26, 10, 2353, 2361, Oct. 2009, [Peer-reviewed]
English, Scientific journal - Grouped nucleotide polymorphism: A major contributor to genetic variation in Arabidopsis
Jianchang Du; Tingting Gu; Hao Tian; Hitoshi Araki; Yong-Hua Yang; Dacheng Tian
GENE, 426, 1-2, 1, 6, Dec. 2008, [Peer-reviewed]
English, Scientific journal - The pattern of insertion/deletion polymorphism in Arabidopsis thaliana
Wen Zhang; Xiaoqin Sun; Huizhong Yuan; Hitoshi Araki; Jue Wang; Dacheng Tian
MOLECULAR GENETICS AND GENOMICS, 280, 4, 351, 361, Oct. 2008, [Peer-reviewed]
English, Scientific journal - Single-nucleotide mutation rate increases close to insertions/deletions in eukaryotes
Dacheng Tian; Qiang Wang; Pengfei Zhang; Hitoshi Araki; Sihai Yang; Martin Kreitman; Thomas Nagylaki; Richard Hudson; Joy Bergelson; Jian-Qun Chen
NATURE, 455, 7209, 105, U70, Sep. 2008, [Peer-reviewed]
English, Scientific journal - Fitness of hatchery-reared salmonids in the wild
Hitoshi Araki; Barry A. Berejikian; Michael J. Ford; Michael S. Blouin
EVOLUTIONARY APPLICATIONS, 1, 2, 342, 355, May 2008, [Peer-reviewed]
English - Molecular evolution of pathogenicity-island genes in Pseudomonas vitidiflava
Hitoshi Araki; Flideki Innan; Martin Kreitman; Joy Bergelson
GENETICS, 177, 2, 1031, 1041, Oct. 2007, [Peer-reviewed]
English, Scientific journal - Genetic effects of captive breeding cause a rapid, cumulative fitness decline in the wild
Hitoshi Araki; Becky Cooper; Michael S. Blouin
SCIENCE, 318, 5847, 100, 103, Oct. 2007, [Peer-reviewed]
English, Scientific journal - Intraspecific genetic variations, fitness cost and benefit of RPW8, a disease resistance locus in Arabidopsis thaliana
Undral Orgil; Hitoshi Araki; Samantha Tangchaiburana; Robert Berkey; Shunyuan Xiao
Genetics, 176, 4, 2317, 2333, Aug. 2007, [Peer-reviewed]
English, Scientific journal - A molecular isolation mechanism associated with high intra-specific diversity in rice
Sihai Yang; Ke Jiang; Hitoshi Araki; Jing Ding; Yong-Hua Yang; Dacheng Tian
GENE, 394, 1-2, 87, 95, Jun. 2007, [Peer-reviewed]
English, Scientific journal - Highly asymmetric rice genomes
Jing Ding; Hitoshi Araki; Qiang Wang; Pengfei Zhang; Sihai Yang; Jian-Qun Chen; Dacheng Tian
BMC GENOMICS, 8, Jun. 2007, [Peer-reviewed]
English, Scientific journal - A potential bias in the temporal method for estimating N-e in admixed populations under natural selection
Hitoshi Araki; Robin S. Waples; Michael S. Blouin
MOLECULAR ECOLOGY, 16, 11, 2261, 2271, Jun. 2007, [Peer-reviewed]
English, Scientific journal - Contrasting patterns of evolution between allelic groups at a single locus in Arabidopsis
Jing Ding; Houlong Cheng; Xinqing Jin; Hitoshi Araki; Yonghua Yang; Dacheng Tian
GENETICA, 129, 3, 235, 242, Mar. 2007, [Peer-reviewed]
English, Scientific journal - Effective population size of steelhead trout: influence of variance in reproductive success, hatchery programs, and genetic compensation between life-history forms
Hitoshi Araki; Robin S. Waples; William R. Ardren; Becky Cooper; Michael S. Blouin
MOLECULAR ECOLOGY, 16, 5, 953, 966, Mar. 2007, [Peer-reviewed]
English, Scientific journal - Reproductive success of captive-bred steelhead trout in the wild: Evaluation of three hatchery programs in the Hood River
Hitoshi Araki; William R. Ardren; Erik Olsen; Becky Cooper; Michael S. Blouin
CONSERVATION BIOLOGY, 21, 1, 181, 190, Feb. 2007, [Peer-reviewed]
English, Scientific journal - Presence/absence polymorphism for alternative pathogenicity islands in Pseudomonas viridiflava, a pathogen of Arabidopsis
Hitoshi Araki; Dacheng Tian; Erica M. Goss; Katrin Jakob; Solveig S. Halldorsdottir; Martin Kreitman; Joy Bergelson
Proceedings of the National Academy of Sciences of the United States of America, 103, 15, 5887, 5892, Apr. 2006, [Peer-reviewed]
English, Scientific journal - Unique evolutionary mechanism in R-genes under the presence/absence polymorphism in Arabidopsis thaliana
Jingdan Shen; Hitoshi Araki; Lingling Chen; Jian Qun Chen; Dacheng Tian
Genetics, 172, 2, 1243, 1250, Feb. 2006, [Peer-reviewed]
English, Scientific journal - Unbiased estimation of relative reproductive success of different groups: Evaluation and correction of bias caused by parentage assignment errors
Hitoshi Araki; Michael S. Blouin
Molecular Ecology, 14, 13, 4097, 4109, Nov. 2005, [Peer-reviewed]
English, Scientific journal - Genetic coadaptation of the amylase gene system in Drosophila melanogaster: Evidence for the selective advantage of the lowest AMY activity and of its epistatic genetic background
H. Araki; S. Yoshizumi; N. Inomata; T. Yamazaki
Journal of Heredity, 96, 4, 388, 395, Jul. 2005, [Peer-reviewed]
Scientific journal - Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes
T. Zhou; Y. Wang; J. Q. Chen; H. Araki; Z. Jing; K. Jiang; J. Shen; D. Tian
Molecular Genetics and Genomics, 271, 4, 402, 415, May 2004, [Peer-reviewed]
Scientific journal - Pseudomonas viridiflava and P. syringae - Natural pathogens of Arabidopsis thaliana
Katrin Jakob; Erica M. Goss; Hitoshi Araki; Tam Van; Martin Kreitman; Joy Bergelson
Molecular Plant-Microbe Interactions, 15, 12, 1195, 1203, 01 Dec. 2002, [Peer-reviewed]
English, Scientific journal - Signature of balancing selection in Arabidopsis
Dacheng Tian; Hitoshi Araki; Eli Stahl; Joy Bergelson; Martin Kreitman
Proceedings of the National Academy of Sciences of the United States of America, 99, 17, 11525, 11530, 20 Aug. 2002, [Peer-reviewed]
English, Scientific journal - Molecular evolution of duplicated amylase gene regions in Drosophila melanogaster: Evidence of positive selection in the coding regions and selective constraints in the cis-regulatory regions
Hitoshi Araki; Nobuyuki Inomata; Tsuneyuki Yamazaki
Genetics, 157, 2, 667, 677, 2001, [Peer-reviewed]
Scientific journal - Bottleneck effect on evolutionary rate in the nearly neutral mutation model
Hitoshi Araki; Hidenori Tachida
Genetics, 147, 2, 907, 914, Oct. 1997, [Peer-reviewed]
English, Scientific journal
- Functional eradication of largemouth bass in Kanabara Dam Lake, Japan
坪井潤一; 片野修; 水本寛基; 水本寛基; 荒木仁志, 保全生態学研究, 28, 2, 2024 - Migratory fish species as a measure of habitat connectivity
福島路生; 中嶋信美; 山川茜; 速水将人; 荒木仁志; 飯塚毅; RAND P.S., 国立環境研究所研究プロジェクト報告 SR(Web), 138, 2021 - Is there data bias in biological photos with location information collected in a citizen participatory research?
佐橋玄記; 丸山緑; 有賀望; 森田健太郎; 岡本康寿; 向井徹; 水本寛基; 植田和俊; 藤井和也; 渡辺恵三; 大熊一正; 荒木仁志; 佐橋玄記; 丸山緑; 有賀望; 森田健太郎; 岡本康寿; 向井徹; 水本寛基; 植田和俊; 藤井和也; 渡辺恵三; 大熊一正; 荒木仁志, 日本生態学会大会講演要旨(Web), 67th, 2020 - 環境DNAを用いた生物分布推定とシシャモ遡上分布への応用
八柳哲; 荒木仁志, 水産海洋研究, 84, 3, 2020 - Development and application of environmental DNA techniques for evaluating distribution and population status of rare/invasive species
荒木仁志; 山中裕樹; 内井喜美子; 加三千宣; 土居秀幸, 個体群生態学会大会プログラム・講演要旨集(Web), 36th, 2020 - eDNA-based haplotype analysis on nagaremon-charr
水本寛基; 亀甲武志; 荒木仁志, 日本魚類学会年会講演要旨, 54th (CD-ROM), 2020 - 環境DNA手法のコウモリ調査への応用
岩澤大地; 荒木仁志, 日本生態学会大会講演要旨(Web), 66th, 2019 - 治山ダムの魚道設置や切り下げによる魚類の応答と魚類相の変化
速水将人; 長坂晶子; 長坂有; 福島路生; 下田和孝; 卜部浩一; 川村洋司; 小野理; 中嶋信美; 荒木仁志; 水本寛基, 日本生態学会大会講演要旨(Web), 66th, 2019 - 環境DNAを用いた魚類の現存量推定
卜部浩一; 水本寛基; 山口文; 荒木仁志, 日本生態学会大会講演要旨(Web), 66th, 2019 - 環境DNAから探る,日本固有種シシャモの遡上と分布
八柳哲; 神戸崇; 水本寛基; 小林由美; 坂田雅之; 源利文; 石田良太郎; 新居久也; 荒木仁志, 日本生態学会大会講演要旨(Web), 66th, 2019 - 札幌・豊平川の野生サケと市民の持続可能な共存に向けて~市民活動・札幌ワイルドサーモンプロジェクト~
丸山緑; 水本寛基; 有賀望; 岡本康寿; 森田健太郎; 荒木仁志, 日本進化学会大会プログラム・講演要旨集(Web), 21st, 2019 - 環境DNA手法のコウモリ調査への応用
岩澤大地; 荒木仁志, 日本進化学会大会プログラム・講演要旨集(Web), 21st, 2019 - 環境DNAを用いた絶滅危惧種イトウの遺伝的多様性・分布・生態系同時評価手法の開発
荒木仁志, 旭硝子財団研究助成成果発表会, 2019, 2019 - Understanding aquatic biodiversity in Hokkaido using environmental DNA
荒木仁志; 水本寛基, 海洋と生物, 40, 1, 35‐39, 15 Feb. 2018
Japanese - 環境DNAを通して観る北海道の水圏生物—Understanding aquatic biodiversity in Hokkaido using environmental DNA—特集 環境DNAが拓く魚類生態研究の未来
荒木 仁志; 水本 寛基, 海洋と生物 = Aquabiology, 40, 1, 35, 39, Feb. 2018
生物研究社, Japanese - 環境DNA技術を用いたイトウの季節回遊行動の推定
水本寛基; 荒木仁志; 宮正樹, 日本生態学会大会講演要旨(Web), 65th, ROMBUNNO.F01‐10 (WEB ONLY), 2018
Japanese - 環境DNAを通して観る,北の海の生物と多様性
荒木仁志; 神戸崇; 水本寛基; 鎌田祥子; 南波聡子; 矢部衞; 大橋優季; 池田実; 峰岸有紀; 佐土哲也; 宮正樹, 日本魚類学会年会講演要旨, 50th, 139, 14 Sep. 2017
Japanese - 環境DNA : 水から垣間見る魚類相と生物多様性
荒木 仁志, アクアネット = Aqua net : 産地と消費地をネットする水産情報誌, 20, 2, 46, 50, Feb. 2017
湊文社, Japanese - The application of eDNA technique for the monitoring of marine fish species
KONDOH Michio; ARAKI Hitoshi; KASAI Akihide; MASUDA Reiji; MINAMOTO Toshifumi; MIYA Masaki, 個体群生態学会大会プログラム・講演要旨集, 33rd, 2017 - サケの性的サイズ二型:野生魚と放流魚の違い
森田健太郎; 佐藤俊平; 荒木仁志, 日本生態学会大会講演要旨(Web), 64th, 2017 - 環境DNAを用いた生物フロンティアの開拓
荒木仁志; 神戸崇; 鎌田頌子; 宮正樹; 佐土哲也; 池田実; 佐藤行人; 峰岸有紀; 永野優季; 矢部衞, 日本進化学会大会プログラム・講演要旨集(Web), 18th, ROMBUNNO.O‐1E‐1 (WEB ONLY), 25 Aug. 2016
Japanese - 環境DNAを用いたニホンザリガニ生息場の推定における季節影響
池田幸資; 土居秀幸; 田中一典; 川井唯史; 荒木仁志; 高原輝彦; 源利文; 鈴木透; 小泉逸郎; 根岸淳二郎, 土木学会年次学術講演会講演概要集(CD-ROM), 71st, ROMBUNNO.VII‐122, 01 Aug. 2016
Japanese - 絶滅危惧淡水魚イトウの産卵遡上の特徴
福島路生; RAND P.S; 水本寛基; 荒木仁志, 日本生態学会大会講演要旨(Web), 63th, P2‐322 (WEB ONLY), 2016
Japanese - 環境DNAと音響ビデオカメラから見るイトウ遡上量の経日変化
水本寛基; 荒木仁志; 福島路生; 卜部浩一, 日本生態学会大会講演要旨(Web), 63th, T20‐3 (WEB ONLY), 2016
Japanese - 環境DNA研究の実践と課題:生態学フロンティアへの挑戦
荒木仁志; 神戸崇; 鎌田頌子; 源利文; 佐藤行人; 宮正樹, 日本生態学会大会講演要旨(Web), 63th, T20‐1 (WEB ONLY), 2016
Japanese - 北の海に未知なる生命と生物多様性を探る
荒木仁志; 宮正樹; 池田実; 矢部衞; 永野優季; 神戸崇; 鎌田頌子; 佐土哲也; 峰岸有紀; 佐藤行人; 佐藤俊平; MASLENIKOV Katherine, 日本進化学会大会プログラム・講演要旨集(Web), 17th, ROMBUNNO.W6‐2 (WEB ONLY), 20 Aug. 2015
Japanese - MiFish, a set of universal primers for metabarcoding environmental DNA from fishes: detection of > 230 species from aquarium tanks and coral reefs in the subtropical western North Pacific
Masaki Miya; Yukuto Sato; Tsukasa Fukunaga; Tetsuya Sado; Keiichi Sato; Toshifumi Minamoto; Satoshi Yamamoto; Hiroaki Yamanaka; Hitoshi Araki; Michio Kondoh; Wataru Iwasaki, GENOME, 58, 5, 257, 257, May 2015, [Peer-reviewed]
English, Summary international conference - 環境DNAメタバーコーディング解析のためのパイプライン開発
佐藤行人; 荒木仁志; 宮正樹; 佐土哲也; 峰岸有紀; 岩崎渉, 日本進化学会大会プログラム・講演要旨集(Web), 17th, 2015 - 魚類環境DNA用ユニバーサルプライマーの開発と次世代シーケンサを用いた環境水分析法の確立
宮正樹; 佐藤行人; 福永津嵩; 佐土哲也; 佐藤圭一; 源利文; 山中裕樹; 荒木仁志; 岩崎渉, 日本魚類学会年会講演要旨, 47th, 26, 14 Nov. 2014
Japanese - 遺伝子発現の確率的な挙動が生物集団へ与える影響
峯田克彦; 松本知高; 長田直樹; 荒木仁志, 日本分子生物学会年会プログラム・要旨集(Web), 35th, 1P-0013 (WEB ONLY), 2012
Japanese - A review essay of a genetic evaluation program on the natural reproductive success of hatchery-born fish using DNA-based parentage assignments
荒木 仁志, Fish genetics and breeding science, 39, 1, 31, 35, Oct. 2009
Abstract:In June 2009. I gave a talk about our recent studies on the Hood River steelhead trout at the ISGA X (the 10th International Symposium on Genetics in Aquaculture) in Bangkok. Thailand. In this project. I and group members of Blouin lab in Oregon State University conducted genetic evaluations of reproductive success in hatchery-born fish in the wild. This article is a review summary of my presentation. We used a DNA-based parentage assignment method. in which 8 highly polymorphic microsatellite makers were genotyped in order to reconstruct the pedigree of entire populations and estimate individual reproductive success (number of adult offspring returned to the river). We found (1) number of generations in which hatchery fish spent in captivity has a strong negative impact on their natural reproductive success. and that (2) there is a carry-over effect of captive breeding that reduces the reproductive success of wild-born fish. which had captive-reared parents spawned in the wild. These findings strongly suggest a possible negative effect of hatchery stocking on the conservation of the wild population. In addition. I demonstrate that modern molecular tools and methods can provide much more insights into fish genetics and breeding science than ever obtained or expected., 水産育種研究会, Japanese - キイロショウジョウバエのケージ集団を用いたアミラーゼ遺伝子についての選択実験に関する分子集団遺伝学的解析 II
荒木仁志; 吉住敏; 猪股伸幸; 山崎常行, 日本遺伝学会大会プログラム・予稿集, 71st, 114, 24 Sep. 1999
Japanese - Molecular population genetic analysis of selection experiment on amylase gene using cage group of Drosophila melanogaster.
荒木仁志; 吉住敏; 猪股伸幸; 松尾義則; 山崎常行, 日本遺伝学会大会プログラム・予稿集, 70th, 105, Sep. 1998
Japanese - Theoretical research on the effects of natural selection and population changes to gene substituting speed.
荒木仁志; 舘田英典, 日本遺伝学会大会プログラム・予稿集, 67th, 86, Oct. 1995
Japanese
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- 国際交流Ⅰ, 2024年, 学士課程, 国際本部
- 生物資源科学特講, 2024年, 学士課程, 農学部
- 環境と人間, 2024年, 学士課程, 全学教育
- 基礎遺伝学, 2024年, 学士課程, 農学部
- 生物資源科学実験Ⅰ, 2024年, 学士課程, 農学部
- Understanding migratory fishes in rivers and coasts
Grants-in-Aid for Scientific Research
01 Apr. 2023 - 31 Mar. 2027
荒木 仁志; 峰岸 有紀; 仲岡 雅裕
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 23H00329 - 環境核酸を用いた絶滅危惧種イトウの有効集団サイズ・遺伝子発現推定技術の開発と実践
科学研究費助成事業
30 Jun. 2023 - 31 Mar. 2026
荒木 仁志; 坂田 雅之
日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 23K18145 - 環境DNAを用いたサケ科魚類および回遊性魚類の種内多型解析手法の開発
科学研究費助成事業 基盤研究(B)
01 Apr. 2020 - 31 Mar. 2023
荒木 仁志
本研究プロジェクトは、生態学分野の新しいツールの一つである「環境DNA」を用いて魚類の生物分布や遺伝的多様性を同時検出・評価することを目的としている。水圏では、水を汲みその中に含まれるDNAを解析する環境DNA技術は捕獲や目視によらない非侵襲的な種判別技術として目覚ましい発展を遂げている。しかしそのDNAには種内多型情報も含まれるため、これに基づく集団遺伝解析への応用可能性がある。そこで本研究では回遊性魚類を主なターゲットに環境DNA技術を核ゲノム中のマイクロサテライトマーカーに適用し、同マーカーの種内多型情報に基づく集団遺伝解析手法の確立をめざす。
R3年度は北海道・千歳川における定期採水を継続すると共に、そこで得られた環境DNAサンプルを分析、サケ由来の環境DNAの検出を行うと同時に種内多型を検出するためのマイクロサテライトマーカーの開発を行った。その結果、作成した22個のマーカー候補のうち21個についてはサケの種内多型を確認でき、18個については千歳川由来の環境DNAからも対立遺伝子のPCR増幅が見られることが分かった。ただし一部のマーカーは他のサケ科魚類でも僅かながら誤増幅が起こることも判明したため、これらの種が共存しうる河川での使用時には注意が必要となる。一方、これまで研究室で収集した北海道内広域サンプルの解析の結果から、地域変異差の大きい純淡水魚を対象とした環境DNA解析では、ハードルの高い核DNAではなく、相対的に収集の容易なミトコンドリアDNAを用いた種内多型解析が可能な場合あることが分かってきた。今後はこれら回遊魚・純淡水魚を含めた環境DNA解析を行うことで、この技術の種内多型解析研究への応用可能性を広く明らかにしていく。なお、これらの結果は2021年11月に研究代表者が主催した国際学会、環境DNA学会第四回大会において発表を行った。
日本学術振興会, 基盤研究(B), 北海道大学, 20H03005 - Monitoring of fauna of the Northern Territory, with a special attention to the sika deer and the European mink
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2020 - 31 Mar. 2023
大舘 智志; 荒木 仁志; 河合 久仁子
内容
昨年度(令和3年)は、令和2年度に続いて新型コロナ対策のために北方領土に渡航することができなかった。そのかわりに研究計画にも述べてあるように北方領土に隣接した北海道東部の根室市や浜中町に哺乳動物相の捕獲調査を行った。それによると令和3年度は真無盲腸目のトガリネズミ類の個体数密度が低く、特にオオアシトガリネズミの密度がかなり減っていた。一方で浜中町ではイタチ科のラッコが海で繁殖しているのを確認した。ニホンジカについては道東地域では例年通りの高密度であった。一方、現地に直接おもむけない北方領土についての情報は国後島のクリリスク自然保護区の所長や所員から得た。それによると2021年の春の段階ではエゾシカが国後島に数頭存在しているのが確認されたが、色丹島や歯舞諸島などの他の北方領土では確認されていない。また国後島でも繁殖は確認されていない。国後島のヨーロッパミンクについては情報不足ではあるが生息していることは確かであった。事前に採集してある国後島と択捉島の環境DNAの分析では、哺乳類についてはエゾシカとヨーロッパミンクは検出されなかった。一方、淡水魚類については淡水性のサケ・マス類などが検出された。
意義
北方領土に隣接する北海道東部では例年通りにシカの密度は高かった。このことはいつでも北方領土へのさらなるシカの移住が起こりうることを示している。また国後島ではシカは前年に確認した個体が生存していると思われるが、繁殖していないということは、まだ当地でシカが増加するという段階にはないことを示している。一方、ヨーロッパミンクは現地調査ができない状況が最大の研究上の障害であることが明確になった。
重要性
モニタリングは継続することが重要であるが、現地に赴けない状況下では現地の協力機関や協力者との密な情報のやり取りにより必要な知見をえることができる。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20K06086 - Understanding genetic diversity, population structure and natural distribution of a critically endangered salmonid species (Sakhalin taimen) using environmental DNA
Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows
10 Nov. 2017 - 31 Mar. 2020
荒木 仁志; CAMPBELL MATTHEW
本研究は日本最大の淡水魚で絶滅危惧種であるイトウの生態に関する新たな知見を得るため、昨今進展目覚ましい環境DNAを用いた手法を核DNAの種内多型に応用する技術開発を目標としている。現在環境DNA技術は主に細胞内小器官の一つであるミトコンドリアのDNAを基に解析されているが、ミトコンドリアDNAは一般に種内多型に乏しく、同一種内でおこる変異の多くを反映していない。そこで、環境水中の核DNA情報をより高密度で集積することにより、「一すくいの水から希少種・野外集団の遺伝的多様性評価」へとつなげるのが本研究プロジェクトの狙いである。この技術開発が実現すれば、イトウに限らず希少種を捕獲したり傷つけたりすることなく彼らの遺伝的多様性を評価できるばかりか、過去のボトルネック、集団間の遺伝的交流といった歴史についても推定が可能となることが期待される。
これにはRAD-seqと呼ばれる手法を用い、本解析に適したゲノムスケールでの核DNAマーカー探索を行う必要があるため、H29年度はこの解析を実施するためのサンプル収集と予備的な解析を実施した。共同研究者であるマシュー・キャンベル(JSPS外国人特別研究員)の着任がH29年10月末であったことから、昨年度は5カ月間という限られた期間ではあったが、研究協力者らの協力の下、約30個体のイトウ組織サンプルからのDNA抽出が実現し、これらのサンプルを基にRAD-seq法による核DNAマーカー開発の準備が整いつつある。
Japan Society for the Promotion of Science, Grant-in-Aid for JSPS Fellows, Hokkaido University, 17F17391 - Environmental DNA survey for estimating migratory fish distribution and evaluating impacts of river structures
Grants-in-Aid for Scientific Research
01 Apr. 2017 - 31 Mar. 2020
Araki Hitoshi
In this study, we developed environmental DNA detection systems for understanding distribution and movement of migratory fish species and evaluating impacts of river structures on them. We found that hidden distributions of endangered Sakhalin taimen and Shishamo smelt and their migratory patterns, as well as Hokkaido-wide distributions of >50 freshwater and anadromous fish species including invasive fish species. Together with estimations of environmental parameters, our results suggested that dams and weirs cause local extinctions of salmonid species and that development of fish ladder had positive effects on fish migration.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 17H03623 - An ecological maintenance mechanism of genetic diversity focusing on spatial structure and population dynamics of the Hokkaido vole
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2014 - 31 Mar. 2017
SAITOH Takashi
The relationship between mtDNA and ncDNA diversity was analyzed in order to test a theoretical prediction that mtDNA diversity should be much lower than ncDNA diversity. In contrast to this prediction, mtDNA diversity was higher than ncDNA diversity in many mammalian and avian populations. This was attributed to higher mutation rate in mtDNA, while high variation of the diversities was interpreted as a result of population fluctuation. The relationship between mtDNA and ncDNA diversity differed between mammalian and avian populations: mtDNA diversity was higher in mammals than in birds. This difference could be explained by different dispersal tendency. Male-biased dispersal prevails in mammals, where female-biased dispersal is common in birds. These results supported my hypothesis that sub-population structure plays an important role in maintaining genetic diversity.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 26291086 - Understanding genetics and value of wild salmon run
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2014 - 31 Mar. 2017
Araki Hitoshi; SATO Shunpei; KOIZUMI Itsuro; O' MALLEY Kathleen
Salmon is very popular in Japan. While majority of fish in a market is hatchery-reared, ecology of wild salmon is largely unknown. In this study, we investigated genetic basis of wild chum salmon, which returned to their mother rivers at different timings. We found that in some rivers in Japan, early-run population and late-run population are clearly distinct both in timing and the population genetic structure. In addition, the population genetic structure in a river where hatchery fish have been imported was similar to that in its source, despite the fact that the river holds large amount of wild population at this point of time. These results suggest that salmon populations with different run timings are genetically differentiated even within a same river, and that they should be managed differently.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 26292102 - Non-invasive monitoring and biomass estimation of salmonid fish using environmental DNA
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
01 Apr. 2014 - 31 Mar. 2017
ARAKI Hitoshi; KONDO Michio; DOI Hideyuki; SATO Shunpei
Monitoring and biomass estimation of wild organisms are of central importance in conservation biology. However, aquatic organisms are difficult to monitor because of their difficulties in catch and visual observations in the wild.
In this study, we aimed to establish a new molecular method for monitoring and biomass estimation of salmonid species in the wild. We use environmental DNA, which is defined as DNA from environmental media (e.g., water for fish), in order to identify and estimate the amount of wild organisms nearby. Results from our rearing experiments, together with our trials in rivers and the ocean, suggest that environmental DNA we detected indeed reflects the presence/absence and spatio-temporal variation in salmonid species.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 26640136 - シロイヌナズナの抵抗性遺伝子と病原体抗原遺伝子の共進化に関する研究
科学研究費助成事業 特別研究員奨励費
2002 - 2004
荒木 仁志
シロイヌナズナで見つかっている病原体認識遺伝子、R遺伝子は、LRRと呼ばれるシグナル伝達に関わると思われる領域を持ち、バクテリアの持つAvr遺伝子の産物を特異的に認識して免疫反応を引き起こす。しかしながら、R遺伝子、Avr遺伝子の分子進化上の対応関係については未だ明らかにされていない。本研究は、シロイヌナズナのR遺伝子の一つ、RPS5の種内変異を解析すると共に、自然界における主要な病原体と思われるPseudomonas viridiflavaのAvr遺伝子を同定し、種内変異を基にR遺伝子との共進化のパターンについて解析を行うことにより、植物の抵抗性遺伝子とバクテリアの抗原遺伝子の間の相互作用、植物の免疫システムの獲得プロセスを解明することを目的としている。
本年度は、昨年度より研究代表者が共同研究者であるシカゴ大学生態進化学講座のメンバーと共に携わってきたRPS5の種内変異の解析を行い、この遺伝子が、これまで一般に受け入れられていた「軍拡競争仮説」の予測に反して分子進化上、長期にわたって「病原体抵抗性・非抵抗性」の種内多型を選択的に維持していることを示した。また、自家受精型であるこのシロイヌナズナにおいて、上記の遺伝子で選択的種内多型を維持したことがゲノム上の隣接する遺伝子に与える影響を、周辺部位のDNA多型から推定したところ、その影響の範囲がおよそ10KB以内であったことも示した。さらに、バクテリアの一種、Pseudomonas viridiflava及びPseudomonas syringaeがシロイヌナズナに寄生する天然の寄生バクテリアであることを示すに当たって、寄生に不可欠なプロセスに関与する複数の遺伝子がこれらのバクテリアに存在することを示した.。
日本学術振興会, 特別研究員奨励費, 九州大学, 02J08855 - キイロショウジョウバエのアミラーゼ遺伝子におけるエサ環境と適応度に関する研究
科学研究費助成事業 特別研究員奨励費
1999 - 2000
荒木 仁志
日本学術振興会, 特別研究員奨励費, 九州大学, 99J03231
