Jul. 2024, The 20th Annual Meeting of Japanese Society for the Study of Vascular Anomalies, Best Presentation Award　　　　　　　　　　　　　　　
Comparative study of PIK3CA genotype and mTOR pathway protein phosphorylation in patients with Klippel-Trenaunay syndrome
Kosuke Ishikawa

May 2023, Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Hokkaido University, UHS-PRS Clinical Research Paper Award　　　　　　　　　　　　　　　
Ishikawa Kosuke

Oct. 2021, The 17th Annual Meeting of Japanese Society for the Study of Vascular Anomalies, Young Investigator's Award　　　　　　　　　　　　　　　
Endoscopy-assisted X-ray-guided sclerotherapy for treating venous malformations of the oral and pharyngeal regions
Kosuke Ishikawa

Dec. 2020, Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Hokkaido University, UHS-PRS Basic Researcher Award　　　　　　　　　　　　　　　
Ishikawa Kosuke

May 2019, Japan Society of Plastic and Reconstructive Surgery, Journal of Japan Society of Plastic and Reconstructive Surgery Best Article Award　　　　　　　　　　　　　　　
A 10-year analysis of the contribution of plastic surgeons to team surgery
Ishikawa Kosuke, Ishikawa K, et al. [A 10-year analysis of the contribution of plastic surgeons to team surgery]. Journal of Japan Society of Plastic and Reconstructive Surgery 38, 277-282, 2018, Official journal, Japan

Jun. 2014, WOCN Society's 46th Annual Conference, Nashville, Tennessee, Case Study Merit Award　　　　　　　　　　　　　　　
Management of extensive peristomal skin ulcer associated with colon cancer: Usefulness of negative pressure wound therapy for skin graft fixation
Kosuke Ishikawa, Ishikawa K, et al. Management of extensive peristomal skin ulcer associated with colon cancer: Usefulness of negative pressure wound therapy for skin graft fixation., International society, United States

Jun. 2014, Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Hokkaido University, Young Researcher Award “La Primavera”　　　　　　　　　　　　　　　
Ishikawa Kosuke, Ishikawa K, et al. Preliminary experience with intraoperative near-infrared fluorescence imaging in percutaneous sclerotherapy of soft-tissue venous malformations. Dermatol Surg 39, 907-912, 2013, Others, Japan

Apr. 2013, Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Hokkaido University, Young Researcher Award “La Primavera”　　　　　　　　　　　　　　　
Ishikawa Kosuke, Ishikawa K, et al. [Macrocystic lymphatic malformation of the labia minora: A case report]. Journal of Japan Society of Plastic and Reconstructive Surgery 32, 401-405, 2012
Ishikawa K, et al. [Rapidly involuting congenital hemangioma of the trunk: Two case reports]. Journal of Japan Society of Plastic and Reconstructive Surgery 32, 856-860, 2012, Others, Japan

Extracellular matrix modulating effects of amnion‐derived mesenchymal stem cells on aging skin wounds in α‐Klotho knockout mice
Sasaki Y, Ohnishi S, Takahashi H, Ishikawa K, Miura T, Funayama E, Okubo N, Yamamoto Y, Maeda T
Geriatrics & Gerontology International, 25, 5, 701, 708, Wiley, 14 Apr. 2025, [Peer-reviewed]
English, Scientific journal, Aim

Wounds in the elderly are frequently recalcitrant and chronic as a result of the effects of skin aging and associated complications. The objective of this study is to utilize an α‐Klotho knockout (KO) mice wound model to assess the capacity of amnion‐derived mesenchymal stem cells (AMSCs) to facilitate wound healing in aging skin.

Methods

AMSCs were applied topically to the wound after extraction and gelatinization of the conditioned medium (CM). Animal experiments were performed with two distinct mouse strains: α‐Klotho KO mice and wild‐type mice. Full‐thickness skin defect models with a diameter of 8 mm were created by incising the skin on the left and right sides of the dorsum. On day 8 after wound creation, the mice were sacrificed, and wound tissue was collected for analysis through histological and immunohistochemical evaluations, as well as through quantitative polymerase chain reaction.

Results

The topical application of CM gel to wounds of α‐Klotho KO mice demonstrated that wound healing was significantly higher than that observed in control, reaching the wound closure rate of wild‐type mice on day 8. Additionally, gene expression analysis of wound tissue indicated that AMSC‐CM may regulate extracellular matrix formation and fibrosis. Moreover, histological analysis indicated that AMSC‐CM may facilitate wound contraction of aging skin wounds of α‐Klotho KO mice by inducing myofibroblast differentiation and promoting granulation and collagen formation, which are the primary components of the extracellular matrix.

Conclusions

AMSC‐CM may facilitate wound healing in aging skin of α‐Klotho KO mice by regulating the extracellular matrix. Geriatr Gerontol Int 2025; 25: 701–708.

A novel mouse model for studies of burn wound conversion using a top hat–shaped brass template
Matsuda N, Sato C, Ishikawa K, Miura T, Funayama E, Yamamoto Y, Fuyama K, Ohnishi S, Maeda T
Burns, 51, 2, 107348, Mar. 2025, [Peer-reviewed]
English, Scientific journal

Effective management of continuous salivary flow through a pharyngocutaneous fistula using a negative pressure wound therapy device
Maeda T, Tsushima N, Ishikawa K, Yamamoto Y
JPRAS Open, 43, 74, 78, Elsevier BV, Mar. 2025, [Peer-reviewed]
English, Scientific journal

Pitfalls in ultrasound diagnosis of vascular malformations: A retrospective review of 14 nonvascular tumors treated as vascular malformations
Mitamura S, Ishikawa K, Sasaki Y, Murao N, Sasaki S
Diagnostics, 15, 4, 506, 19 Feb. 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

3D scanner’s potential as a novel tool for lymphedema measurement in mouse hindlimb models
Seo D, Ito R, Ishikawa K, Miura T, Yamamoto Y, Onodera Y, Nishioka S, Ito YM, Fuyama K, Maeda T
Scientific Reports, 15, 1, 3747, Springer Science and Business Media LLC, 30 Jan. 2025, [Peer-reviewed]
English, Scientific journal

Hypoxia-inducible factor prolyl hydroxylase inhibitor roxadustat accelerates wound healing in a mouse hind limb lymphedema model
Ishikawa K., Hoshino Y., Osawa M., Funayama E., Miura T., Hojo M., Sasaki Y., Sasaki S., Yamamoto Y., Maeda T.
Advances in Wound Care, 24 Jan. 2025, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal, Objective: Drugs regulating hypoxia-inducible factor (HIF)-1α have not been investigated for wound healing in lymphedema. Therefore, we examined the effects of drug modulation of HIF-1α activity for wound healing in our previously developed mouse model of nonirradiated hind limb lymphedema. Approach: Mouse hind limb lymphedema models (n = 17) and a sham group (n = 6) were created using 8- to 10-week-old male C57BL/6N mice. Mice with hind limb lymphedema were randomized into experimental groups receiving roxadustat, 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1), or dimethyl sulfoxide and were given intraperitoneal injections every 2 days for up to 2 weeks. Four days after the surgery, an 8-mm diameter full-thickness skin wound was created in the hind limb. The number of days required for wound closure and the percentage of wounds closed were measured. Skin samples taken at wound creation were evaluated by histological and molecular analysis. Results: Administration of roxadustat accelerated wound healing, whereas YC-1 delayed it, with a significant decrease and increase in skin thickness, respectively. The relative mRNA expression of Hif1α, matrix metalloproteinase-3, and interleukin-6 was significantly higher in the roxadustat group and that of metalloproteinase-9 was significantly lower in the roxadustat group compared with the control group. Innovation: This study is the first to demonstrate delayed wound healing in a mouse model of hind limb lymphedema and the first to demonstrate the promotion of significant wound healing through the use of roxadustat. Conclusion: Roxadustat exerts wound-healing effects and may promote the regulation of extracellular matrix remodeling via gene expression in hind limb lymphedema wound models.

Actionable gene alterations identified in patients with malignant melanoma by targeted sequencing in Japan
Noguchi T, Ariga S, Moku R, Kikuchi J, Amano T, Maeda T, Ishikawa K, Maeda T, Shiiya A, Goda T, Ohhara Y, Hagio K, Saito Y, Hatanaka KC, Hatanaka Y, Taguchi J, Takeuchi S, Shimizu Y, Kinoshita I
JCO Precision Oncology, 9, e2400437, 17 Jan. 2025, [Peer-reviewed]
English, Scientific journal

Physiological and functional restoration of the modiolus using a double tendon sling in patients with full-thickness oral defects
Maeda T, Hayash T, Ishikawa K, Miura T, Funayama E, Yamamoto Y
Annals of Plastic Surgery, 94, 1, 70, 74, Ovid Technologies (Wolters Kluwer Health), Jan. 2025, [Peer-reviewed]
English, Scientific journal, Background

Reconstruction of the oral modiolus should be considered for facial defects involving the lip and cheek. We have performed free flap reconstruction using a novel technique that includes a vertical and a horizontal sling for full-thickness defects involving the oral commissure.

Patients and Methods

We used a free radial forearm flap and the palmaris longus tendon to correct lip and cheek defects in 6 patients (3 men, 3 women; age, 55–84 years). The tendon was divided into 2 portions for use as the double sling. One sling was used to connect the upper and lower stumps of the orbicularis oris in the vertical direction and the other to connect the vertical sling and masseter muscle. The outcomes assessed were esthetic appearance and functional results.

Results

The facial defects ranged in size from 3.0 cm × 4.0 cm to 7.0 cm × 6.0 cm and the mucosal defects from 1.5 cm × 4.0 cm to 6.5 cm × 5.0 cm. The length of the harvested tendon ranged from 11.5 to 13.5 cm. All flaps survived without complications. Good esthetic outcomes were achieved (excellent facial appearance, n = 1; no lip deviation or drooping, n = 5). Functionally, all patients had acceptable mouth opening and were able to eat a regular diet.

Conclusions

Good physiological and functional results can be achieved using a double tendon sling for reconstruction of the modiolus in patients with full-thickness defects involving the oral commissure.

Synergistic anti-tumor effects of oncolytic virus and anti-programmed cell death protein 1 antibody combination therapy: For suppression of lymph node and distant metastasis in a murine melanoma model
Sasaki Y., Maeda T., Hojo M., Miura T., Ishikawa K., Funayama E., Okada K., Yamamoto Y.
Biochemical and Biophysical Research Communications, 740, 151011, Dec. 2024, [Peer-reviewed]
English, Scientific journal

Comparison of leg volume ratio between inguinal lymphadenectomy and inguino-pelvic lymphadenectomy in patients with skin cancer of the lower extremity
Maeda T, Ishikawa K, Hayashi T, Furukawa H, Miura T, Hojo M, Funayama E, Yamamoto Y
Journal of Plastic, Reconstructive & Aesthetic Surgery, 99, 397, 405, Elsevier BV, Dec. 2024, [Peer-reviewed]
English, Scientific journal

Hypoxic culture enhances the antimicrobial activity of amnion-derived mesenchymal stem cells, thereby reducing bacterial load and promoting wound healing in diabetic mice
Ishii R, Ohnishi S, Hojo M, Ishikawa K, Funayama E, Miura T, Okubo N, Okada K, Yamamoto Y, Maeda T
Biochemical and Biophysical Research Communications, 739, 150903, Elsevier BV, Oct. 2024, [Peer-reviewed]
English, Scientific journal

Distinct roles of omental and latissimus dorsi flaps for blocking infection pathways and protecting a left ventricular assist device
Seo D, Maeda T, Ooka T, Miura T, Ishikawa K, Funayama E, Wakasa S, Yamamoto Y
The Heart Surgery Forum, 27, 9, E998, E1002, Forum Multimedia Publishing LLC, 10 Sep. 2024, [Peer-reviewed]
English, Scientific journal, Left ventricular assist devices (LVADs) are vital for managing severe heart failure in transplant-ineligible patients, but device exposure and infection pose significant challenges. This report details a 42-year-old man with dilated cardiomyopathy and bronchial asthma who presented with an externalized LVAD following a HeartMate II to HeartMate III exchange due to malfunction. Our tailored surgical strategy treated the LVAD driveline and main body as two distinct parts. This distinction is crucial, as major upstream infections typically originate from the driveline. We applied flaps to these parts for different purposes, maximizing their unique characteristics. The omental flap, chosen for its flexibility, blood supply, and immunological activation upon foreign body contact, covered the driveline. The latissimus dorsi flap provided vascularity and mechanical protection for the LVAD. Additionally, we review the omentum's basic physiological aspects, which are often unfamiliar to clinicians. Infection has not recurred in 6 months postoperatively, demonstrating the approach's effectiveness.

Leukocytoclastic vasculitis-induced lower leg ulceration in the course of immune checkpoint inhibitor therapy for advanced malignant melanoma: a case report
Seo D, Ariga S, Ishikawa K, Miura T, Moku R, Funayama E, Yamamoto Y, Maeda T
Journal of Wound Management and Research, 20, 2, 168, 172, Korean Wound Management Society, 30 Jun. 2024, [Peer-reviewed]
English, Scientific journal, Immune checkpoint inhibitors (ICIs) have expanded therapeutic options for advanced malignancies, offering new hope for conditions once deemed untreatable. However, the advent of ICIs has introduced a spectrum of immune-related adverse events (irAEs), including leukocytoclastic vasculitis (LCV), a rare but significant complication. This case report describes development of LCV after treatment with nivolumab and ipilimumab in a 70-year-old man with malignant melanoma, highlighting the diagnostic and management challenges of such irAEs. Despite extensive investigation, conventional pathology failed to identify the immune complexes typically associated with LCV. The clinical presentation, alongside a detailed medical history and the exclusion of infections, medications, and autoimmune diseases, was crucial in establishing a diagnosis. Ulcer resolution following discontinuation of ICI therapy and initiation of steroids further support the conclusion that LCV was an irAE in this patient. This case underscores the need for vigilant monitoring for irAEs for the variable onset after ICI therapy and the importance of thorough history-taking to guide diagnosis and treatment. With ICIs becoming increasingly prevalent in oncology, the incidence of ICI-induced ulcers like LCV is expected to rise, necessitating heightened awareness and multidisciplinary approaches to patient care.

Fluoroscopy- and endoscopy-guided transoral sclerotherapy using foamed polidocanol for oropharyngolaryngeal venous malformations in a hybrid operation room: A case series
Ishikawa K, Maeda T, Funayama E, Murao N, Miura T, Sasaki Y, Seo D, Mitamura S, Oide S, Yamamoto Y, Sasaki S
Journal of Clinical Medicine, 13, 8, 2369, 18 Apr. 2024, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
English, Scientific journal

Lymphatic drainage patterns of malignant skin tumors in the head and neck region: a single-center retrospective study
Nishio T, Maeda T, Ishikawa K, Murao N, Fuyama K, Yamamoto Y, Hayashi T
International Journal of Clinical Oncology, 29, 5, 582, 591, Springer Science and Business Media LLC, 30 Mar. 2024, [Peer-reviewed]
English, Scientific journal

Refinement of surgery for postaxial polysyndactyly of the toes: Esthetic outcome in Japanese feet
Ishikawa K, Furukawa H, Yamamoto Y, Funayama E, Miura T, Maeda T
Annals of Plastic Surgery, 92, 6, 694, 699, Ovid Technologies (Wolters Kluwer Health), 27 Mar. 2024, [Peer-reviewed], [Lead author]
English, Scientific journal, Purpose

Treatment for polysyndactyly of the toes aims at cosmetic improvement but the lateroplantar rotation of the new fifth toe remains challenging. This study evaluated our novel surgical procedure for postaxial polysyndactyly of the toes.

Materials and Methods

Patients with postaxial polysyndactyly involving the fourth, fifth, and sixth toes treated in 2007 to 2017 with a minimum follow-up duration of 1 year were retrospectively investigated. Our aims of surgery for this condition were to avoid excessive lateroplantar rotation of the new fifth toe by using a proximally elongated plantar “shark-fin flap” and to make the tip of this toe appear to be naturally pointing inward by using the dog-ear component of the flap on the tip of the toe. The excess skin of the shark-fin flap was grafted onto the lateral surface of the fourth toe. Lateroplantar rotation of the fifth toe in these patients was compared with that in photographs of the feet of 96 normal 4-year-old children.

Results

A total of 11 feet in 10 patients (6 male, 4 female; mean age 1.3 years) were analyzed. Syndactyly between the fourth and fifth toes was complete in 3 feet, incomplete at the level of the distal interphalangeal joint of the fifth toe in 5, and incomplete at the level of the proximal interphalangeal joint of the fifth toe in 3. Lateroplantar rotation of the fifth toe, evaluated by the mean angle between 2 intersecting lines extending from the proximal nail fold of the third and fifth toes, was 25 ± 10° in normal feet and 0 ± 12° in operated feet with polysyndactyly. The absolute left-right difference in this angle was 7 ± 5° in normal children and 22 ± 12° in patients with polysyndactyly. Valgus deformity of the new fifth toe improved in all patients during a mean postoperative follow-up of 3.8 years.

Conclusions

Using our procedure, no excessive lateroplantar rotation has been observed when the tip of the fifth toe is inclined inward using a dog-ear flap component. This procedure could be useful in patients in whom the cosmetic outcome is a priority.

Negative pressure wound therapy with instillation and dwell time in patients on hemodialysis: A retrospective study of 65 cases
Kusajima EG, Ishikawa K, Funayama E, Miura T, Horiuchi K, Matsui S, Saito T, Matsui H, Yamamoto Y, Maeda T
International Journal of Surgical Wound Care, 5, 1, 1, 7, 01 Mar. 2024, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

The prospective natural history study of patients with intractable venous malformation and Klippel–Trenaunay syndrome to guide designing a proof-of-concept clinical trial for novel therapeutic intervention
Fujino A, Kuniyeda K, Nozaki T, Ozeki M, Ohyama T, Sato I, Kamibeppu K, Tanaka A, Uemura N, Kanmuri K, Nakamura K, Kobayashi F, Suenobu S, Nomura T, Hayashi A, Nagao M, Kato A, Aramaki-Hattori N, Imagawa K, Ishikawa K, Ochi J, Horiuchi S, Nagabukuro H
Lymphatic Research and Biology, 22, 1, 27, 36, Mary Ann Liebert Inc, 01 Feb. 2024, [Peer-reviewed]
English, Scientific journal

Burn conversion possibly caused by COVID-19 infection: A case report
Seo D, Maeda T, Yamamoto Y, Funayama E, Miura T, Matsuda N, Ishikawa K
Burns Open, 8, 1, 5, 7, Jan. 2024, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Intranodal lymphangiography with lipiodol as a diagnostic and therapeutic approach for spontaneous cervical chyle leak
Sasaki Y, Sakuhara Y, Sasaki S, Maeda T, Yamamoto Y, Ishikawa K
Clinical Case Reports, 11, 12, e8161, 19 Dec. 2023, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Partially involuting congenital hemangioma with pyogenic granuloma‐induced rapid progression following incisional biopsy in infancy: A case report
Sasaki Y, Ishikawa K, Miura T, Funayama E, Yamamoto Y, Maeda T
Clinical Case Reports, 11, 10, e7941, Wiley, 17 Oct. 2023, [Peer-reviewed]
English, Scientific journal, Key Clinical Message

We present a case of partially involuting congenital hemangioma (PICH) that showed rapid growth with ulceration after incisional biopsy. PICH does not typically grow after birth. However, if growth occurs due to stimuli like biopsy, it is essential to consider the possibility of pyogenic granuloma complication.

Targeted next-generation sequencing for detection of PIK3CA mutations in archival tissues from patients with Klippel–Trenaunay syndrome in an Asian population
Sasaki Y, Ishikawa K, Hatanaka KC, Oyamada Y, Sakuhara Y, Shimizu T, Saito T, Murao N, Onodera T, Miura T, Maeda T, Funayama E, Hatanaka Y, Yamamoto Y, Sasaki S
Orphanet Journal of Rare Diseases, 18, 1, 270, Springer Science and Business Media LLC, 04 Sep. 2023, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Abstract

Background

Klippel–Trenaunay syndrome (KTS) is a rare slow-flow combined vascular malformation with limb hypertrophy. KTS is thought to lie on the PIK3CA-related overgrowth spectrum, but reports are limited. PIK3CA encodes p110α, a catalytic subunit of phosphatidylinositol 3-kinase (PI3K) that plays an essential role in the PI3K/AKT/mammalian target of rapamycin (mTOR) signaling pathway. We aimed to demonstrate the clinical utility of targeted next-generation sequencing (NGS) in identifying PIK3CA mosaicism in archival formalin-fixed paraffin-embedded (FFPE) tissues from patients with KTS.

Results

Participants were 9 female and 5 male patients with KTS diagnosed as capillaro-venous malformation (CVM) or capillaro-lymphatico-venous malformation (CLVM). Median age at resection was 14 years (range, 5–57 years). Median archival period before DNA extraction from FFPE tissues was 5.4 years (range, 3–7 years). NGS-based sequencing of PIK3CA achieved an amplicon mean coverage of 119,000x. PIK3CA missense mutations were found in 12 of 14 patients (85.7%; 6/8 CVM and 6/6 CLVM), with 8 patients showing the hotspot variants E542K, E545K, H1047R, and H1047L. The non-hotspot PIK3CA variants C420R, Q546K, and Q546R were identified in 4 patients. Overall, the mean variant allele frequency for identified PIK3CA variants was 6.9% (range, 1.6–17.4%). All patients with geographic capillary malformation, histopathological lymphatic malformation or macrodactyly of the foot had PIK3CA variants. No genotype–phenotype association between hotspot and non-hotspot PIK3CA variants was found. Histologically, the vessels and adipose tissues of the lesions showed phosphorylation of the proteins in the PI3K/AKT/mTOR signaling pathway, including p-AKT, p-mTOR, and p-4EBP1.

Conclusions

The PI3K/AKT/mTOR pathway in mesenchymal tissues was activated in patients with KTS. Amplicon-based targeted NGS could identify low-level mosaicism from low-input DNA extracted from FFPE tissues, potentially providing a diagnostic option for personalized medicine with inhibitors of the PI3K/AKT/mTOR signaling pathway.

Therapeutic potential of the prolyl hydroxylase inhibitor roxadustat in a mouse hindlimb lymphedema model
Hoshino Y, Osawa M, Funayama E, Ishikawa K, Miura T, Hojo M, Yamamoto Y, Maeda T
Lymphatic Research and Biology, 21, 4, 372, 380, Mary Ann Liebert Inc, 22 Aug. 2023, [Peer-reviewed]
English, Scientific journal

Utility of color doppler ultrasonography in monitoring of a free jejunal flap
Maeda T, Ishikawa K, Oda Y, Kano S, Tsushima N, Sakashita T, Homma A, Yamamoto Y, Funayama E
The Laryngoscope, 133, 12, 3361, 3369, Wiley, 29 Jun. 2023, [Peer-reviewed], [Invited]
English, Scientific journal

Restoration of lymph flow by flap transfer can prevent severe lower extremity lymphedema after inguino-pelvic lymphadenectomy
Maeda T, Yamamoto T, Hayashi T, Furukawa H, Ishikawa K, Miura T, Hojo M, Funayama E
Surgery Today, 53, 5, 588, 595, Springer Science and Business Media LLC, May 2023, [Peer-reviewed]
English, Scientific journal

Development of a simultaneous and noninvasive measuring method using high-frame rate videography and motion analysis software for the assessment of facial palsy recovery in a rat model
Miura T, Yamamoto Y, Funayama E, Ishikawa K, Maeda T
Journal of Plastic, Reconstructive and Aesthetic Surgery, 82, 211, 218, Elsevier BV, 17 Apr. 2023, [Peer-reviewed]
English, Scientific journal

Effectiveness and safety of percutaneous sclerotherapy using absolute ethanol and/or polidocanol for maxillofacial venous malformations involving the masticatory muscles: A case series
Ishikawa K, Sasaki S, Furukawa H, Maeda T, Miura T, Sasaki Y, Yamamoto Y, Funayama E
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 135, 3, 355, 362, Elsevier BV, Mar. 2023, [Peer-reviewed], [Lead author]
English, Scientific journal

Efficacy of hyperbaric oxygen after microtia reconstruction using costal cartilage: A retrospective case-control study
Murao N, Oyama A, Yamamoto Y, Funayama E, Ishikawa K, Maeda T
Undersea and Hyperbaric Medicine, 50, 4, 413, 419, Undersea and Hyperbaric Medical Society (UHMS), 01 Jan. 2023, [Peer-reviewed]
English, Scientific journal, Introduction: Microtia reconstruction with autologous costal cartilage framework grafting is challenging because the three-dimensional structure of the ear is highly complex, and meeting the high aesthetic demands of patients can be difficult. If the skin flap overlying the framework is thinned to achieve a smooth and accentuated outline, a poor blood supply in the thin skin flap may lead to skin necrosis, exposure of the framework, and poor surgical results. Hyperbaric oxygen (HBO2) therapy can promote the healing of complex wounds and flaps. This study sought to determine the effectiveness of HBO2 therapy for prevention of postoperative complications after framework grafting in microtia reconstruction. Methods: We retrospectively evaluated postoperative complications and compared outcomes in pediatric patients who underwent costal cartilage grafting for microtia reconstruction at our institution between 2011 and 2015 according to whether or not they received postoperative HBO2 therapy. HBO2 therapy was applied once daily for a total of 10 sessions starting on the first postoperative day. Results: During the study period, eight patients received HBO2 therapy after costal cartilage grafting and 12 did not. There was no significant difference in the incidence of postoperative ulcers. However, the incidence of framework exposure was lower and the healing time was shorter in patients who received HBO2 therapy than in those who did not. Discussion: HBO2 therapy can be used safely in pediatric patients to reduce postoperative complications and improve the aesthetic outcome of microtia reconstruction. After costal cartilage grafting, HBO2 therapy should be considered as adjuvant therapy.

Sentinel node restoration by vascularized lymph node transfer in mice
Kusajima EG, Yamamoto Y, Ishikawa K, Miura T, Funayama E, Osawa M, Takagi R, Maeda T
Microsurgery, 44, 1, e30981, Wiley, 02 Nov. 2022, [Peer-reviewed]
English, Scientific journal, Abstract

Background

Recent reports have indicated that vascularized lymph node transfer (VLNT) may improve the impaired immunity in lymphedema but there has been no report concerning anti‐cancer immunity. In the early tumor immune response, dendritic cells (DCs) participate in tumor recognition and antigen presentation in local lymphatics. Here, we investigated the impact of VLNT on DC dynamics against cancer in mouse models.

Methods

Forty‐seven 8‐week‐old C57BL/6 N male mice were divided into three surgical groups: a VLNT model in which a vascularized inguinal lymph node (LN) flap was transferred into the ipsilateral fossa after a popliteal LN was removed; a LN dissection (LND) model in which the popliteal LN was dissected; and a control model in which a skin incision was made at the popliteal fossa and an ipsilateral inguinal LN was removed. Postoperative lymphatic flows were observed by indocyanine green lymphography and B16‐F10‐luc2 mouse melanoma were implanted into the ipsilateral footpad. The proportion of DCs in the transplanted nodes was measured by CD11c immunohistochemistry using digital imaging analysis 4 days after cancer implantation. Metastases to the lungs and LNs were quantitatively evaluated by luciferase assay 4 weeks after cancer implantation.

Results

After VLNT, lymphatic reconnection was observed in 59.2% of mice. The proportion of DCs was significantly higher in the VLNT group with lymphatic reconnection (8.6% ± 1.0%) than in the naïve LN (4.3% ± 0.4%) (p < .001). The tumor burden of lung metastases was significantly less in the VLNT group with lymphatic reconnection compared with the LND group (p = .049).

Conclusions

Metastasis decreased in mice with reconnected lymphatics after VLNT. A possible explanation was that lymphatic restoration may have contributed to the tumor immune response by allowing DC migration to LNs.

Streptococcal toxic shock syndrome in a pediatric patient with intramuscular venous malformation in the neck
Matsuda N, Ishikawa K, Funayama E, Mitamura S, Sasaki S, Yamamoto Y, Maeda T
The Journal of Emergency Medicine, 63, 3, e72, e76, Elsevier BV, Oct. 2022, [Peer-reviewed]
English, Scientific journal

Lingual arteriovenous malformation with bleeding treated with polidocanol foam sclerotherapy using intestinal forceps to control blood flow
Sasaki Y, Sasaki S, Ishikawa K
Journal of Craniofacial Surgery, 33, 3, e292, e293, Ovid Technologies (Wolters Kluwer Health), May 2022, [Peer-reviewed], [Corresponding author]
English, Scientific journal

A novel treatment of widespread empyema necessitatis
Otsuka S, Ujiie H, Ishikawa K, Miura T, Wakasa S, Kato T
The Annals of Thoracic Surgery, 112, 6, e471, e471, Elsevier {BV}, Sep. 2021, [Peer-reviewed]
English, Scientific journal

Breast reconstruction via fat grafting for a patient with bilateral congenital amastia (Finlay‐Marks syndrome)
Riri Ito, Yuhei Yamamoto, Taku Maeda, Kosuke Ishikawa, Emi Funayama
Congenital Anomalies, 61, 4, 142, 143, Wiley, Jul. 2021, [Peer-reviewed]
English, Scientific journal

Topical application of conditioned medium from hypoxically cultured amnion-derived mesenchymal stem cells promotes wound healing in diabetic mice
Takahashi H, Ohnishi S, Yamamoto Y, Hayashi T, Murao N, Osawa M, Maeda T, Ishikawa K, Sakamoto N, Funayama E
Plastic & Reconstructive Surgery, 147, 6, 1342, 1352, Ovid Technologies (Wolters Kluwer Health), Jun. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Mesenchymal stem cells or their conditioned medium improve chronic wound healing, and their effect is enhanced by hypoxia. Diabetic foot ulcers are chronic wounds characterized by abnormal and delayed healing, which frequently require amputation. The authors evaluated the effect of topical application of conditioned medium from hypoxically cultured amnion-derived mesenchymal stem cells on wound healing in diabetic mice. METHODS: Amnion-derived mesenchymal stem cells were cultured under 21% oxygen to prepare normoxic conditioned medium and under 1% oxygen to prepare hypoxic conditioned medium. Hydrogels containing standard medium, normoxic conditioned medium, or hypoxic conditioned medium were topically applied to excisional wounds of mice with streptozotocin-induced diabetes. Ulcer tissues were harvested on day 9; immunohistochemical and quantitative polymerase chain reaction analyses were performed to analyze angiogenesis, inflammatory cell infiltration, and expression levels of inflammation-related genes. RESULTS: Hypoxic conditioned medium significantly enhanced wound closure, increased capillary density and epithelization, and reduced macrophage infiltration. It also tended to reduce the infiltration of neutrophils and enhance the infiltration of regulatory T cells; it showed a tendency to downregulate the expression of the inflammation-related genes interleukin-1β, interleukin-6, chemokine ligand 1, and chemokine ligand 2. Normoxic conditioned medium exhibited similar effects, although they were of lesser magnitude than those of hypoxic conditioned medium. CONCLUSIONS: Hydrogels containing hypoxically cultured, amnion-derived mesenchymal stem cell conditioned medium accelerated wound healing in diabetic mice by enhancing angiogenesis, accelerating epithelization, and suppressing inflammation. Therefore, topical application of amnion mesenchymal stem cell-derived hypoxic conditioned medium could be a novel treatment for diabetic foot ulcers.

Long-term outcomes and recurrence-free interval after the treatment of keloids with a standardized protocol
Maeda T, Funayama E, Yamamoto Y, Murao N, Osawa M, Ishikawa K, Hayashi T
Journal of Tissue Viability, 30, 1, 128, 132, Elsevier BV, Feb. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Recurrence rates of keloids have generally been reported at one time point. However, the longer the duration after treatment, the greater the likelihood that such lesions will recur. In this study, we analysed the time to recurrence during long-term follow-up. MATERIAL AND METHODS: We retrospectively reviewed recurrence-free interval in 52 patients with keloid (age 8-79 years) who had been treated between June 2006 and January 2011 using a standardised protocol developed by our group. RESULTS: Mean duration of follow-up was 37.5 (range, 7-120) months in patients with keloid. Kaplan-Meier survival curves revealed a statistically significant difference in recurrence-free interval between ear keloids and keloids excluding ear keloids. Recurrence rate for keloids was high in the first 2 years after treatment. CONCLUSIONS: Kaplan-Meier analysis was useful for understanding the tendency of recurrence of keloids after treatment using a standardised protocol.

Auricular arteriovenous malformation with macrotia treated with transcatheter arterial embolization, polidocanol foam sclerotherapy and subsequent otoplasty following resection
Ishikawa K, Fujita M, Sasaki S
Journal of Craniofacial Surgery, 32, 5, e489, e490, Ovid Technologies (Wolters Kluwer Health), 14 Jan. 2021, [Peer-reviewed], [Lead author]
English, Scientific journal

Iatrogenic third-degree burn caused by off-label use of an infrared radiant heat lamp in a patient with accidental hypothermia
Ishikawa K, Maeda T, Hayashi T, Shichinohe R, Murao N, Hojo M, Ito R, Osawa M, Yamamoto Y, Funayama E
Burns Open, 5, 1, 21, 24, Elsevier {BV}, Jan. 2021, [Peer-reviewed], [Lead author]
English, Scientific journal

Application of negative-pressure wound therapy after radical excision of soft tissue malignancy: to obtain a better esthetic result with a less invasive reconstructive technique
Maeda T, Murao N, Hayashi T, Funayama E, Osawa M, Ishikawa K, Yamamoto Y
International Journal of Surgical Wound Care, 1, 2, 47, 52, Japan Society for Surgical Wound Care, Sep. 2020, [Peer-reviewed]
English, Scientific journal

Ulcerated infantile hemangioma of the hard palate: diagnostic treatment with oral propranolol
Ishikawa K, Fujita M, Takeda T, Mitamura S, Nishio T, Funayama E, Hayashi T, Osawa M, Maeda T, Yamamoto Y, Sasaki S
The Journal of Craniofacial Surgery, 31, 6, e600, e602, Ovid Technologies (Wolters Kluwer Health), 03 Jul. 2020, [Peer-reviewed], [Lead author]
English, Scientific journal

Wound care for calciphylaxis: a novel approach for wound management with negative pressure wound therapy with instillation and dwell time
Kusajima EG, Yamamoto Y, Murao N, Hayashi T, Osawa M, Maeda T, Ishikawa K, Horiuchi K, Matsui S, Saito T, Matsui H, Funayama E
International Journal of Surgical Wound Care, 1, 1, 20, 26, Japan Society for Surgical Wound Care, Jun. 2020, [Peer-reviewed]
English, Scientific journal

Japanese clinical practice guidelines for vascular anomalies 2017
Mimura H, Akita S, Fujino A, Jinnin M, Ozaki M, Osuga K, Nakaoka H, Morii E, Kuramochi A, Aoki Y, Arai Y, Aramaki N, Inoue M, Iwashina Y, Iwanaka T, Ueno S, Umezawa A, Ozeki M, Ochi J, Kinoshita Y, Kurita M, Seike S, Takakura N, Takahashi M, Tachibana T, Chuman K, Nagata S, Narushima M, Niimi Y, Nosaka S, Nozaki T, Hashimoto K, Hayashi A, Hirakawa S, Fujikawa A, Hori Y, Matsuoka K, Mori H, Yamamoto Y, Yuzuriha S, Rikihisa N, Watanabe S, Watanabe S, Kuroda T, Sugawara S, Ishikawa K, Sasaki S
The Journal of Dermatology, 47, 5, e138, e183, May 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, The objective was to prepare guidelines to perform the current optimum treatment by organizing effective and efficient treatments of hemangiomas and vascular malformations, confirming the safety and systematizing treatment, employing evidence-based medicine techniques and aimed at improvement of the outcomes. Clinical questions (CQ) were decided based on the important clinical issues. For document retrieval, key words for published work searches were set for each CQ, and work published from 1980 to the end of September 2014 was searched in PubMed, Cochrane Library and Japana Centra Revuo Medicina databases. The strengths of evidence and recommendations acquired by systematic reviews were determined following the Medical Information Network Distribution System technique. A total of 33 CQ were used to compile recommendations and the subjects included efficacy of resection, sclerotherapy/embolization, drug therapy, laser therapy, radiotherapy and other conservative treatment, differences in appropriate treatment due to the location of lesions and among symptoms, appropriate timing of treatment and tests, and pathological diagnosis deciding the diagnosis. Thus, the Japanese Clinical Practice Guidelines for Vascular Anomalies 2017 have been prepared as the evidence-based guidelines for the management of vascular anomalies.

Japanese clinical practice guidelines for vascular anomalies 2017
Mimura H, Akita S, Fujino A, Jinnin M, Ozaki M, Osuga K, Nakaoka H, Morii E, Kuramochi A, Aoki Y, Arai Y, Aramaki N, Inoue M, Iwashina Y, Iwanaka T, Ueno S, Umezawa A, Ozeki M, Ochi J, Kinoshita Y, Kurita M, Seike S, Takakura N, Takahashi M, Tachibana T, Chuman K, Nagata S, Narushima M, Niimi Y, Nosaka S, Nozaki T, Hashimoto K, Hayashi A, Hirakawa S, Fujikawa A, Hori Y, Matsuoka K, Mori H, Yamamoto Y, Yuzuriha S, Rikihisa N, Watanabe S, Watanabe S, Kuroda T, Sugawara S, Ishikawa K, Sasaki S
Japanese Journal of Radiology, 38, 4, 287, 342, Apr. 2020, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The objective was to prepare guidelines to perform the current optimum treatment by organizing effective and efficient treatments of hemangiomas and vascular malformations, confirming the safety, and systematizing treatment, employing evidence-based medicine (EBM) techniques and aimed at improvement of the outcomes. Clinical questions (CQs) were decided based on the important clinical issues. For document retrieval, key words for literature searches were set for each CQ and literature published from 1980 to the end of September 2014 was searched in Pubmed, Cochrane Library, and Japana Centra Revuo Medicina (JCRM). The strengths of evidence and recommendations acquired by systematic reviews were determined following the Medical Information Network Distribution System (MINDS) technique. A total of 33 CQs were used to compile recommendations and the subjects included efficacy of resection, sclerotherapy/embolization, drug therapy, laser therapy, radiotherapy, and other conservative treatment, differences in appropriate treatment due to the location of lesions and among symptoms, appropriate timing of treatment and tests, and pathological diagnosis deciding the diagnosis. Thus, the Japanese Clinical Practice Guidelines for Vascular Anomalies 2017 have been prepared as the evidence-based guidelines for the management of vascular anomalies.

Japanese clinical practice guidelines for vascular anomalies 2017
Mimura H, Akita S, Fujino A, Jinnin M, Ozaki M, Osuga K, Nakaoka H, Morii E, Kuramochi A, Aoki Y, Arai Y, Aramaki N, Inoue M, Iwashina Y, Iwanaka T, Ueno S, Umezawa A, Ozeki M, Ochi J, Kinoshita Y, Kurita M, Seike S, Takakura N, Takahashi M, Tachibana T, Chuman K, Nagata S, Narushima M, Niimi Y, Nosaka S, Nozaki T, Hashimoto K, Hayashi A, Hirakawa S, Fujikawa A, Hori Y, Matsuoka K, Mori H, Yamamoto Y, Yuzuriha S, Rikihisa N, Watanabe S, Watanabe S, Kuroda T, Sugawara S, Ishikawa K, Sasaki S
Pediatrics International, 62, 3, 257, 304, Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, The objective was to prepare guidelines to perform the current optimum treatment by organizing effective and efficient treatments of hemangiomas and vascular malformations, confirming the safety, and systematizing treatment, employing evidence-based medicine (EBM) techniques and aimed at improvement of the outcomes. Clinical questions (CQs) were decided based on the important clinical issues. For document retrieval, key words for literature searches were set for each CQ and literature published from 1980 to the end of September 2014 was searched in Pubmed, Cochrane Library, and Japana Centra Revuo Medicina (JCRM). The strengths of evidence and recommendations acquired by systematic reviews were determined following the Medical Information Network Distribution System (MINDS) technique. A total of 33 CQs were used to compile recommendations and the subjects included efficacy of resection, sclerotherapy/embolization, drug therapy, laser therapy, radiotherapy, and other conservative treatment, differences in appropriate treatment due to the location of lesions and among symptoms, appropriate timing of treatment and tests, and pathological diagnosis deciding the diagnosis. Thus, the Japanese Clinical Practice Guidelines for Vascular Anomalies 2017 have been prepared as the evidence-based guidelines for the management of vascular anomalies.

Squamous cell carcinoma arising in a chronic leg ulcer in Klippel-Trenaunay syndrome after the Charles procedure: A case with 40 years of follow up
Ishikawa K, Funayama E, Yamamoto Y, Furukawa H, Hayashi T, Murao N, Osawa M, Maeda T, Fujita M, Sasaki S
The Journal of Dermatology, 46, 11, e403, e405, Nov. 2019, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal

Advancement flap using excess skin for upper eyelid full-thickness defects
Ito R, Maeda T, Yamamoto Y, Funayama E, Murao N, Osawa M, Ishikawa K, Ikeda M, Hayashi T
Journal of Craniofacial Surgery, 30, 8, 2614, 2616, Ovid Technologies (Wolters Kluwer Health), Nov. 2019, [Peer-reviewed]
English, Scientific journal

Oculo-auriculo-fronto-nasal syndrome with duane retraction syndrome and dysplastic bony structure in the midline of nose
Ito R, Osawa M, Yamamoto Y, Murao N, Hayashi T, Maeda T, Ishikawa K, Oyama A, Funayama E
The Journal of Craniofacial Surgery, 30, 6, e576, e578, Sep. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Oculo-auriculo-fronto-nasal syndrome (OAFNS) is a rare anomaly characterized by features overlapping those of frontonasal dysplasia (FND) and the oculo-auriculo-vertebral spectrum (OAVS).1 The FND features malformation of frontonasal process-derived structures, characterized by anomalies in the central portion of the face. The OAVS is characterized by developmental anomalies of the first and second pharyngeal arches. The OAFNS is a condition with clinical features of both FND and OAVS. Here, the authors present the case of a male with OAFNS who not only exhibited typical OAFNS symptoms but also a dysplastic bony structure that bridged the anterior nasal spine and inferior nasal bones, and unilateral type 3 Duane retraction syndrome (absence of right-eye abduction). Abnormal nasal bones are characteristic of OAFNS; such abnormalities are absent from FND and OAVS. The authors reduced the dysplastic nasal bony structure via open external rhinoplasty, followed by lateral nasal osteotomy when he was 16 years of age. The nasal dorsum appeared natural after surgery and he was satisfied with the result.

Changes in high endothelial venules in lymph nodes after vascularized and nonvascularized lymph node transfer in a murine autograft model
Ishikawa K, Funayama E, Maeda T, Hayashi T, Murao N, Osawa M, Ito R, Furukawa H, Oyama A, Yamamoto Y
Journal of Surgical Oncology, 119, 6, 700, 707, Apr. 2019, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal, BACKGROUND AND OBJECTIVES: Vascularized lymph node transfer (LNT) is gaining popularity in the treatment of lymphedema. However, it is unclear whether the vascularization of transferred lymph nodes (LNs) contributes to functional improvement. High endothelial venules (HEVs) are specialized vessels that allow lymphocytes to enter LNs. In this study, we compared the numbers of HEVs and lymphocytes in LNs after vascularized and nonvascularized LNT. METHODS: Fifty mice were divided into three groups (group 1, pedicled vascularized LNT; group 2, pedicled nonvascularized LNT; group 3, free nonvascularized LNT). Afferent lymphatic reconnection was confirmed by patent blue staining. The transferred LNs were harvested 4 weeks after surgery. HEVs, B-cells, and T-cells were subjected to immunohistochemical staining and quantified. RESULTS: Afferent lymphatic reconnection was observed in 13 of 20 transferred LNs in group 1, 11 of 15 in group 2, and 7 of 15 in group 3. The ratio of dilated/total HEVs in transferred LNs with afferent lymphatic reconnection was significantly higher in group 1 than in groups 2 and 3. No significant differences in numbers of B-cells and T-cells were found in the transferred LNs. CONCLUSIONS: We found that more functional HEVs were preserved in cases with successful afferent lymphatic reconnection after vascularized LNT than after nonvascularized LNT.

Feasibility of pedicled vascularized inguinal lymph node transfer in a mouse model: A preliminary study
Ishikawa K, Maeda T, Funayama E, Hayashi T, Murao N, Osawa M, Furukawa H, Oyama A, Yamamoto Y
Microsurgery, 39, 3, 247, 254, Mar. 2019, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal, PURPOSE: Vascularized lymph node transfer is becoming more common in the treatment of lymphedema, but suitable small animal models for research are lacking. Here, we evaluated the feasibility of pedicled vascularized inguinal lymph node transfer in mice. METHODS: Twenty-five mice were used in the study. An inguinal lymph node-bearing flap with a vascular pedicle containing the superficial caudal epigastric vessels was transferred into the ipsilateral popliteal fossa after excision of the popliteal lymph node. Indocyanine green (ICG) angiography was used to confirm vascularity of the flap. ICG lymphography was performed to evaluate lymphatic flow at 3 and 4 weeks postoperatively. Patent blue dye was injected into the ipsilateral hind paw to observe staining of the transferred lymph node at 4 weeks postoperatively. All transferred lymph nodes were then harvested and histologically evaluated by hematoxylin and eosin staining. RESULTS: In 16 of the 25 mice, ICG lymphography showed reconnection between the transferred lymph node and the afferent lymphatic vessels, as confirmed by patent blue staining. Histologically, these transferred lymph nodes with afferent lymphatic reconnection significantly regressed in size (0.37 +/- 0.24 mm(2) ) and showed clear follicle formation, whereas those without afferent lymphatic reconnection showed less size regression (1.31 +/- 1.17 mm(2) ); the cell population was too dense to allow identification of follicles. CONCLUSIONS: We established a mouse model of vascularized lymph node transfer with predictable afferent lymphatic reconnection. Both the vascularization and reconnection might be necessary for functional regeneration of the transferred lymph node.

Wound-Healing Problems Associated with Combined Vascular Malformations in Klippel-Trenaunay Syndrome
Ishikawa, K., Yamamoto, Y., Funayama, E., Furukawa, H., Sasaki, S.
Advances in Wound Care, 8, 6, 246, 255, 2019, [Peer-reviewed], [Invited], [Lead author], [International Magazine]
English, Scientific journal

Immune-mediated antitumor effect of a transplanted lymph node
Maeda T, Hayashi T, Furukawa H, Iwasaki D, Ishikawa K, Funayama E, Murao N, Osawa M, Oyama A, Yamamoto Y
International Journal of Cancer, 143, 5, 1224, 1235, Sep. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Lymph node (LN) transplantation is a recognized method for reconstruction of the lymphatic system and is used in the clinical setting to treat lymphedema. However, it is unclear whether transplanted LNs contribute to immune surveillance. In our study, we investigated whether a single transplanted non-vascularized LN, defined as a tumor-draining transplanted lymph node (TDTLN), could exert an immune-mediated antitumor effect. LN and lung metastases and primary tumor enlargement were evaluated in mice that were inoculated with B16-F10-luc2 melanoma cells in a hind limb footpad without (group 1) and with (group 2) popliteal lymph node (PLN) resection and in mice that underwent LN transplantation after PLN resection (group 3). The function of a TDTLN (group 3) and a tumor-draining popliteal lymph node (TDPLN; group 1) was evaluated in the context of cancer. LN and lung metastases were significantly aggravated by PLN resection but were significantly decreased by LN transplantation. Immunohistochemistry showed that the TDTLNs retained T-cells and B-cells and fluorescence-activated cell sorting analysis confirmed expansion of lymphocytes in these nodes; however, the degree of expansion in TDTLNs was different from that in TDPLNs. Expression of cytokines associated with immunostimulation was confirmed in the TDTLNs as well as in the TDPLNs. One of the differences in the immune-mediated antitumor effect of the TDPLNs and TDTLNs was ascribed to a difference in the site of lymphocyte homing to peripheral LNs through high endothelial venules. Non-vascularized LN transplantation had an immune-mediated antitumor effect.

Long-term changes in bone height after mandibular reconstruction using a free fibula graft in an elderly population
Ishikawa K, Yamamoto Y, Furukawa H, Ohiro Y, Satoh A, Hayashi T
Journal of Oral and Maxillofacial Surgery, 75, 12, 2682, 2688, W.B. Saunders, 01 Dec. 2017, [Peer-reviewed], [Lead author]
English, Scientific journal

Aneuploidy of a murine immortalized endothelial cell line, MS1
Hida, K., Maishi, N., Annan, D.A.-M., Kondoh, M., Hojo, T., Habiba, U., Ohga, N., Ishikawa, K., Sato, M., Torii, C., Yanagiya, M., Morimoto, M., Hida, Y., Shindoh, M.
Journal of Oral Biosciences, 59, 1, 50, 54, Feb. 2017, [Peer-reviewed]
English, Scientific journal

A case of combined soft tissue and intraosseous venous malformation of the thumb treated with sclerotherapy using a bone marrow aspiration needle
Ishikawa K, Sasaki S, Furukawa H, Nagao M, Iwasaki D, Fujita M, Saito N, Oyama A, Yamamoto Y
Case Reports in Plastic Surgery and Hand Surgery, 2, 2, 37, 39, Informa UK Limited, Mar. 2015, [Peer-reviewed], [Lead author]
English, Scientific journal, Vascular malformations of bone are complex lesions that can cause deformity and pain. A combined soft tissue and intraosseous venous malformation of the left thumb in a girl was treated with two sessions of ethanol sclerotherapy using a bone marrow aspiration needle under fluoroscopic guidance.

Surgical management of extensive peristomal pyoderma gangrenosum associated with colon cancer
Ishikawa K, Minamimoto T, Mizuki T, Furukawa H
Journal of Wound Ostomy & Continence Nursing, 42, 1, 102, 105, Jan. 2015, [Peer-reviewed], [Lead author]
English, Scientific journal

Incisional surgical site infection after elective open surgery for colorectal cancer
Ishikawa K, Kusumi T, Hosokawa M, Nishida Y, Sumikawa S, Furukawa H
International Journal of Surgical Oncology, 2014, 419712, 5 pages, Hindawi Publishing Corporation, 27 Mar. 2014, [Peer-reviewed], [Lead author]
English, Scientific journal

Preliminary experience with intraoperative near-infrared fluorescence imaging in percutaneous sclerotherapy of soft-tissue venous malformations
Ishikawa, K., Sasaki, S., Furukawa, H., Nagao, M., Iwasaki, D., Saito, N., Yamamoto, Y.
Dermatologic Surgery, 39, 6, 907, 912, 01 Jun. 2013, [Peer-reviewed], [Lead author]
English, Scientific journal

Advanced epithelioid malignant peripheral nerve sheath tumor showing complete response to combined surgery and chemotherapy: a case report
Minagawa T, Shioya R, Sato C, Shichinohe R, Yasui G, Ishikawa K, Takahashi H
Case Reports in Oncological Medicine, 2011, 705345, 1, 5, Hindawi Limited, Sep. 2011, [Peer-reviewed]
English, Scientific journal, Malignant peripheral nerve sheath tumor (MPNST) is a rare high-grade soft tissue sarcoma. The epithelioid variant accounts for 5% or less of MPNSTs; the clinical behavior of this variant is unclear. Reports of approximately 40 cases are available in the English literature; however, most reports addressed clinicopathological features rather than therapeutic procedures or clinical courses. We describe a case of a 62-year-old male with an epithelioid MPNST of the left foot. Multiple lung metastases developed after radical surgery on the primary lesion. The response to adjuvant chemotherapy including doxorubicin and ifosfamide was favorable, and thoracoscopic resection was subsequently performed on the remaining three metastases. No evidence of recurrence or metastasis was observed at the 12-month followup after the first operation. Further followup and chemotherapy may be required.

Molecular genetics of hemangiomas and vascular malformations　　　　　　　　　　　　　　　
Kosuke Ishikawa, Yuki Sasaki, Satoru Sasaki, The Japanese Journal of Pediatric Hematology / Oncology, 61, 5, 339, 345, Feb. 2025, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

Fibrosarcomatous dermatofibrosarcoma protuberans in the breast
Marika Fukuda, Kosuke Ishikawa, Taku Maeda, Takahiro Miura, Shintaro Mitamura, Hiromi Okada, Emi Funayama, Yuhei Yamamoto, Japanese Journal of Plastic Surgery, 67, 9, 990, 998, Sep. 2024, [Peer-reviewed]
克誠堂出版(株), Japanese, Introduction scientific journal

A case report of back electrical injury sustained by a high-frequency melting furnace system
Hiroko Ishii, Naoki Murao, Kosuke Ishikawa, Dongkyung Seo, Hiromi Kaida, Takahiro Miura, Taku Maeda, Emi Funayama, Yuhei Yamamoto, Japanese Journal of Burn Injuries, 50, 2, 61, 65, Jun. 2024, [Peer-reviewed]
(一社)日本熱傷学会, Japanese, Introduction scientific journal

Wound healing of frostbite
Kosuke Ishikawa, Takahiro Miura, Taku Maeda, Japanese Journal of Plastic Surgery, 67, 増刊, S64, S66, Jun. 2024, [Peer-reviewed], [Lead author]
克誠堂出版(株), Japanese, Introduction scientific journal

The restoration of lymph flow by flap transfer after inguino-pelvic lymphadenectomy
Taku Maeda, Masahiro Hojo, Kosuke Ishikawa, Emi Funayama, Takahiro Miura, Yuhei Yamamoto, Japanese Journal of Lymphology, 47, 1, 45, 47, Jun. 2024, [Invited]
日本リンパ学会, Japanese, Introduction scientific journal

Review of the long-term treatment outcomes of hemangiomas and vascular malformations and their future perspectives
Satoru Sasaki, Naoki Murao, Kosuke Ishikawa, Japanese Journal of Plastic Surgery, 67, 5, 445, 455, May 2024, [Peer-reviewed], [Invited]
克誠堂出版(株), Japanese, Introduction scientific journal

Molecular genetics of vascular anomalies
Ishikawa K, Sasaki S, Dermatology, 4, 6, 712, 725, Dec. 2023, [Invited], [Lead author], [Domestic magazines]
KAGAKU-HYORONSHA, Japanese, Introduction scientific journal

Propranolol treatment for facial infantile hemangiomas in low-birth-weight infants: two case reports
Shintaro Mitamura, Kosuke Ishikawa, Kazutoshi Cho, Furuse Yuta, Yuichi Nakamura, Ryota Honjo, Taku Maeda, Yuhei Yamamoto, Japanese Journal of Plastic Surgery, 66, 11, 1336, 1345, Nov. 2023, [Peer-reviewed]
克誠堂出版(株), Japanese, Introduction scientific journal

A case of sebaceous epithelioma as sebaceous borderline neoplasm
Toshiyuki Minamimoto, Satoru Munakata, Norihiko Shimoyama, Kosuke Ishikawa, Hakodate Medical Journal, 47, 1, 26, 29, Sep. 2023
市立函館病院, Japanese, Introduction scientific journal

A case of burn scar carcinoma with sacral invasion reconstructed using the gluteus maximus muscle turnover flap
Riku Ishii, Taku Maeda, Kosuke Ishikawa, Takeshi Yamao, Yuhei Yamamoto, Toshihiko Hayashi, Japanese Journal of Surgical Wound Care, 13, 3, 118, 123, Jul. 2022, [Peer-reviewed]
(一社)日本創傷外科学会, Japanese, Introduction scientific journal

A case of foot ulcer caused by neuropathy associated with eosinophilic granulomatosis with polyangiitis
Riku Ishii, Naoki Murao, Kosuke Ishikawa, Emi Funayama, Taku Maeda, Masayuki Osawa, Yuhei Yamamoto, Japanese Journal of Surgical Wound Care, 13, 3, 124, 127, Jul. 2022, [Peer-reviewed]
(一社)日本創傷外科学会, Japanese, Introduction scientific journal

The contribution of plastic surgeons to team surgery: The effect of the new coronavirus pandemic　　　　　　　　　　　　　　　
Norifumi Matsuda, Kosuke Ishikawa, Taku Maeda, Masayuki Osawa, Emi Funayama, Naoki Murao, Toshihiko Hayashi, Yuhei Yamamoto, Journal of Japan Society of Plastic and Reconstructive Surgery, 42, 7, 369, 374, Jul. 2022, [Peer-reviewed]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

Reconstruction with free anterolateral thigh flap following total pharyngolaryngectomy for spontaneous isolated superior mesenteric artery dissection
Taku Maeda, Riku Ishii, Masahiro Hojo, Kosuke Ishikawa, Yuhei Yamamoto, Journal of Japanese Society of Reconstructive Microsurgery, 35, 2, 45, 50, Jun. 2022, [Peer-reviewed]
(一社)日本マイクロサージャリー学会, Japanese, Introduction scientific journal

The effectiveness of the surgical lymphatic regeneration of the lymph node transplantation: from the view point of the restoration of circulation and immunity　　　　　　　　　　　　　　　
Taku Maeda, Kosuke Ishikawa, Naoki Murao, Toshihiko Hayashi, Hiroshi Furukawa, Emi Funayama, Masayuki Osawa, Yuhei Yamamoto, Japanese Journal of Lymphology, 45, 1, 38, 41, Jun. 2022, [Invited]
日本リンパ学会, Japanese, Introduction scientific journal

Vascular malformations and vascular tumors　　　　　　　　　　　　　　　
Yuki Sasaki, Kosuke Ishikawa, Satoru Sasaki, PEPARS, 181, 20, 33, Jan. 2022, [Invited]
(株)全日本病院出版会, Japanese, Introduction scientific journal

Reconstruction of deep burn involving cranial bone using free latissimus dorsi flap
Tomohiro Takeda, Kosuke Ishikawa, Toshihiko Hayashi, Taku Maeda, Riku Ishii, Yuki Sasaki, Masahiro Hojo, Takahiro Miura, Masayuki Osawa, Emi Funayama, Yuhei Yamamoto, Japanese Journal of Burn Injuries, 47, 3, 114, 121, Sep. 2021, [Peer-reviewed]
(一社)日本熱傷学会, Japanese, Introduction scientific journal

Studies on circulatory and immunologic effect of transferred lymph nodes in mouse models of lymph node transfer
Kosuke Ishikawa, Taku Maeda, Erika Guilpain Kusajima, Emi Funayama, Toshihiko Hayashi, Masayuki Osawa, Hiroshi Furukawa, Yuhei Yamamoto, Japanese Journal of Lymphology, 44, 1, 24, 27, Jul. 2021, [Invited], [Lead author]
日本リンパ学会, Japanese, Introduction scientific journal

A case of nodular fasciitis in the inguinal region mimicking infantile fibrosarcoma　　　　　　　　　　　　　　　
Tomohiro Takeda, Kosuke Ishikawa, Shintaro Mitamura, Munezumi Fujita, Satoru Sasaki, Journal of Japan Society of Plastic and Reconstructive Surgery, 41, 6, 332, 338, Jun. 2021, [Peer-reviewed]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

A case of oculoectodermal syndrome　　　　　　　　　　　　　　　
Hiromi Kaida, Naoki Murao, Emi Funayama, Kosuke Ishikawa, Hiroko Takahashi, Yuhei Yamamoto, Journal of Japan Society of Plastic and Reconstructive Surgery, 39, 12, 623, 629, Dec. 2019, [Peer-reviewed]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

A case of wound myiasis
Toshiyuki Minamimoto, Kaori Oishi, Mayumi Wada, Keiko Nagata, Kosuke Ishikawa, Toshihiko Hayashi, Hakodate Medical Journal, 43, 1, 20, 23, Sep. 2019
市立函館病院, Japanese, Introduction other

A case of Facklamia hominis abscess
南本 俊之, 佐藤 多嘉之, 小林 延行, 池町 香澄, 杉本 隆幸, 菅原 拓美, 本田 進, 石川 耕資, 村尾 尚紀, 林 利彦, Skin Surgery, 28, 2, 108, 111, Jun. 2019, [Peer-reviewed]
日本臨床皮膚外科学会, Japanese, Introduction scientific journal

動静脈奇形における組織構築の検討
小山田 ゆみ子, 武田 広子, 志田 啓, 作原 祐介, 清水 匡, 佐々木 了, 石川 耕資, 鈴木 昭, 西岡 典子, 共済医報, 68, 2, 243, 245, May 2019
国家公務員共済組合連合会, Japanese

【患児・家族に寄り添う血管腫・脈管奇形の医療】混合型脈管奇形
佐々木 了, 石川 耕資, PEPARS, 145, 53, 60, Jan. 2019, [Invited]
(株)全日本病院出版会, Japanese, Introduction scientific journal

Evaluation of dog and cat bites in our hospital
Toshiyuki Minamimoto, Susumu Honda, Kikuo Nakashima, Kosuke Ishikawa, Toshihiko Hayashi, Hakodate Medical Journal, 42, 1, 50, 53, Oct. 2018
市立函館病院, Japanese, Introduction other

A 10-year analysis of the contribution of plastic surgeons to team surgery　　　　　　　　　　　　　　　
Kosuke Ishikawa, Hiroshi Furukawa, Akihiko Oyama, Emi Funayama, Toshihiko Hayashi, Naoki Murao, Masayuki Osawa, Taku Maeda, Mitsuru Sekido, Yuhei Yamamoto, Journal of Japan Society of Plastic and Reconstructive Surgery, 38, 6, 277, 282, Jun. 2018, [Peer-reviewed], [Lead author], [Domestic magazines]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

Investigation of vascular malformations in the blood coagulation system: a review of 116 patients at Tonan hospital　　　　　　　　　　　　　　　
Munezumi Fujita, Satoru Sasaki, Kosuke Ishikawa, Emi Funayama, Yuhei Yamamoto, Journal of Japan Society of Plastic and Reconstructive Surgery, 38, 4, 183, 191, Apr. 2018, [Peer-reviewed]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

Experience using bimatoprost on eyelashes
Toshiyuki Minamimoto, Susumu Honda, Kentaro Ono, Erika Kusajima, Kosuke Ishikawa, Toshihiko Hayashi, Hakodate Medical Journal, 41, 1, 63, 66, Oct. 2017
市立函館病院, Japanese, Introduction other

当院の医療関連機器圧迫創傷に関して その傾向と対策
南本 俊之, 本田 進, 水木 猛夫, 古川 尚恵, 寺島 一豪, 大野 健太郎, 石川 耕資, 林 利彦, Skin Surgery, 26, 3, 139, 140, Oct. 2017, [Peer-reviewed]
日本臨床皮膚外科学会, Japanese, Introduction scientific journal

[The financial impact of plastic surgeons in team surgery: A 10-year analysis of surgical revenue in a national university hospital in Japan]　　　　　　　　　　　　　　　
Kosuke Ishikawa, Japanese Journal of Plastic Surgery, 60, 9, 1050, 1051, Sep. 2017, [Peer-reviewed], [Invited]
Japanese

A case of severe hand injury
Toshiyuki Minamimoto, Kentaro Ono, Toshimasa Tennoji, Kikuo Nakashima, Erika Kusajima, Kosuke Ishikawa, Toshihiko Hayashi, Hakodate Medical Journal, 40, 1, 22, 25, Nov. 2016
市立函館病院, Japanese, Introduction other

当院での壊死性筋膜炎症例の検討
南本 俊之, 大野 健太郎, 笠島 浩行, 辻口 直紀, 武山 佳洋, 草島 英梨香, 石川 耕資, 林 利彦, Skin Surgery, 25, 3, 156, 158, Oct. 2016, [Peer-reviewed]
日本臨床皮膚外科学会, Japanese, Introduction scientific journal

An adult case of congenital hemangioma combined with inflammation of the upper arm　　　　　　　　　　　　　　　
Munetomo Nagao, Satoru Sasaki, Seiichiro Ishiyama, Daisuke Iwasaki, Kosuke Ishikawa, Journal of Japan Society of Plastic and Reconstructive Surgery, 36, 6, 282, 286, Jun. 2016, [Peer-reviewed]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

外陰部基底細胞癌の治療経験
草島 英梨香, 石川 耕資, 表 千草, 高橋 紀久子, 木村 中, 外丸 詩野, Skin Cancer, 31, 1, 21, 25, Jun. 2016, [Peer-reviewed]
(一社)日本皮膚悪性腫瘍学会, Japanese, Introduction scientific journal

A case of familial ankyloglossia (tongue-tie)
Toshiyuki Minamimoto, Maki Adachi, Minami Yoda, Tomoko Sakuma, Kosuke Ishikawa, Toshihiko Hayashi, Hakodate Medical Journal, 39, 1, 10, 12, Nov. 2015
市立函館病院, Japanese, Introduction other

形成外科で経験した結核症例の検討
南本 俊之, 足立 真紀, 高橋 隆二, 山添 雅己, 古屋 文康, 石川 耕資, 林 利彦, Skin Surgery, 24, 3, 169, 170, Oct. 2015, [Peer-reviewed]
日本臨床皮膚外科学会, Japanese, Introduction scientific journal

当科における下肢静脈奇形110例の検討
齋藤 典子, 佐々木 了, 清水 匡, 長尾 宗朝, 藤田 宗純, 西岡 典子, 石川 耕資, 形成外科, 58, 6, 679, 686, Jun. 2015, [Peer-reviewed]
克誠堂出版(株), Japanese, Introduction scientific journal

【悪性上皮系腫瘍】踵部に生じた基底細胞癌の1例
辻脇 真澄, 保科 大地, 古屋 和彦, 石川 耕資, 皮膚科の臨床, 57, 7, 1097, 1101, Jun. 2015, [Peer-reviewed]
金原出版(株), Japanese, Introduction scientific journal

[A case of lymphoepithelial cyst of the parotid gland]　　　　　　　　　　　　　　　
Kosuke Ishikawa, Erika Kusajima, Riri Ito, Taku Maeda, Journal of Japan Society of Cranio-Maxillo-Facial Surgery, 31, 2, 76, 80, Jun. 2015, [Peer-reviewed], [Lead author]
(一社)日本頭蓋顎顔面外科学会, Japanese, Introduction scientific journal

A case report : occult breast cancer manifesting as scalp skin metastasis
Kikuko Takahashi, Chu Kimura, Taku Maeda, Kosuke Ishikawa, Riri Ito, Hitoshi Ikeda, Skin Cancer, 29, 2, 195, 200, Nov. 2014, [Peer-reviewed]
(一社)日本皮膚悪性腫瘍学会, Japanese

当院における銃創の治療経験
南本 俊之, 石川 耕資, 中島 菊雄, 佐藤 隆弘, 岩井 里子, 林 利彦, Skin Surgery, 23, 3, 107, 114, Oct. 2014, [Peer-reviewed]
日本臨床皮膚外科学会, Japanese, Introduction scientific journal

ランゲルハンス細胞肉腫の1例
南本 俊之, 杉井 政澄, 工藤 和洋, 下山 則彦, 大東 寛幸, 堤 豊, 足立 真紀, 石川 耕資, 林 利彦, 山本 有平, Skin Surgery, 23, 3, 128, 130, Oct. 2014, [Peer-reviewed]
日本臨床皮膚外科学会, Japanese, Introduction scientific journal

A case of myrmecia
Toshiyuki Minamimoto, Masazumi Sugii, Kazuhiro Kudoh, Norihiko Shimoyama, Kosuke Ishikawa, Hakodate Medical Journal, 38, 1, 27, 29, Oct. 2014
市立函館病院, Japanese, Introduction other

[Clinical Study of basal cell carcinoma of the auricle]　　　　　　　　　　　　　　　
Kikuko Takahashi, Chu Kimura, Kosuke Ishikawa, Erika Kusajima, The Journal of Hakodate Central General Hospital, 16, 11, 14, Sep. 2014
函館中央病院, Japanese

[Evaluation of physical function in patients with intractable leg ulcer: using ankle range of motion, plantar sensation and gait speed]　　　　　　　　　　　　　　　
Atsunori Yuasa, Chu Kimura, Kikuko Takahashi, Kosuke Ishikawa, Erika Kusajima, Masato Ooi, The Journal of Hakodate Central General Hospital, 16, 19, 21, Sep. 2014
函館中央病院, Japanese

[A case of spindle cell squamous cell carcinoma of the scalp]　　　　　　　　　　　　　　　
Kosuke Ishikawa, Chu Kimura, Kikuko Takahashi, Erika Kusajima, Riri Ito, Ryuta Shioya, Daichi Hoshina, Masumi Tsujiwaki, Kazuhiko Furuya, Hitoshi Ikeda, The Journal of Hakodate Central General Hospital, 16, 1, 5, 7, Sep. 2014, [Lead author]
函館中央病院, Japanese, Introduction scientific journal

[Validation of the Laboratory Risk Indicator for Necrotizing Fasciitis (LRINEC) score for Differentiation of Necrotizing Fasciitis and Severe Cellulitis]
Kosuke Ishikawa, Toshiyuki Minamimoto, Kimihito Ichimura, Susumu Honda, Takehiro Warabi, Hiroshi Furukawa, Japanese Journal of Surgical Wound Care, 5, 1, 22, 26, Jan. 2014, [Peer-reviewed], [Lead author]
(一社)日本創傷外科学会, Japanese, Introduction scientific journal

A case of chemical burn due to ammonium hydrogenfluoride
Toshiyuki Minamimoto, Kosuke Ishikawa, Masahiko Odagiri, Satoko Iwai, Toshihiko Hayashi, Hakodate Medical Journal, 37, 1, 23, 25, Oct. 2013
市立函館病院, Japanese, Introduction other

[Differences in clinical findings between necrotizing fasciitis and cellulitis: Two case reports and review of the literature]
Kosuke Ishikawa, Toshiyuki Minamimoto, Motoki Morohara, Hiroyuki Okamoto, Marie Suzuki, Naoki Tsujiguchi, Satoko Iwai, Hakodate Medical Journal, 37, 1, 17, 22, Oct. 2013, [Lead author]
市立函館病院, Japanese, Introduction other

[Rapidly involuting congenital hemangioma of the trunk: Two case reports]
Kosuke Ishikawa, Noriko Saito, Daisuke Iwasaki, Munetomo Nagao, Satoru Sasaki, Journal of Japan Society of Plastic and Reconstructive Surgery, 32, 11, 856, 860, Nov. 2012, [Peer-reviewed], [Lead author]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

[Macrocystic lymphatic malformation of the labia minora: A case report]
Kosuke Ishikawa, Seiichiro Ishiyama, Munetomo Nagao, Noriko Saito, Satoru Sasaki, Journal of Japan Society of Plastic and Reconstructive Surgery, 32, 6, 401, 405, Jun. 2012, [Peer-reviewed], [Lead author]
(一社)日本形成外科学会, Japanese, Introduction scientific journal

われわれの工夫! Moving window法による臍突出・腹壁弛緩一期的形成術
皆川 知広, 安居 剛, 齋藤 亮, 石川 耕資, 形成外科, 54, 9, 1060, 1063, Sep. 2011, [Peer-reviewed]
克誠堂出版(株), Japanese, Introduction scientific journal

[Simultaneous reconstruction of the umbilicus following ablative surgery for the abscess of the urachal remnant]　　　　　　　　　　　　　　　
Kosuke Ishikawa, Tomohiro Minagawa, Go Yasui, Tatsuya Mori, Hiroyuki Kanno, Toshiki Aoyagi, Ai Kawaguchi, Journal of Asahikawa Kosei General Hospital, 20, 2, 109, 112, Dec. 2010, [Lead author]
旭川厚生病院, Japanese, Introduction other

[Surgical management of necrotizing fasciitis]　　　　　　　　　　　　　　　
Ishikawa Kosuke
AL-media 17(5), Nov. 2013, 3, 9-11, Japanese, [Contributor]

〔Major achievements〕Fluoroscopy- and endoscopy-guided transoral sclerotherapy using foamed polidocanol for oropharyngolaryngeal venous malformations in a hybrid operation room: A case series　　　　　　　　　　　　　　　
Ishikawa K, Maeda T, Funayama E, Murao N, Miura T, Yamamoto Y, Sasaki S
25th Workshop of the International Society for the Study of Vascular Anomalies (ISSVA), 08 May 2024, English, Poster presentation
07 May 2024 - 10 May 2024

〔Major achievements〕Targeted next-generation sequencing for detection of PIK3CA mutations in archival tissues from patients with Klippel–Trenaunay syndrome: A retrospective study of 14 patients　　　　　　　　　　　　　　　
Ishikawa K, Sasaki Y, Hatanaka KC, Oyamada Y, Sakuhara Y, Shimizu T, Saito T, Murao N, Onodera T, Miura T, Maeda T, Funayama E, Hatanaka Y, Yamamoto Y, Sasaki S
25th Workshop of the International Society for the Study of Vascular Anomalies (ISSVA), 08 May 2024, English, Poster presentation
07 May 2024 - 10 May 2024

〔Major achievements〕Management of lip arteriovenous malformations with percutaneous polidocanol sclerotherapy: A retrospective study of 20 patients　　　　　　　　　　　　　　　
Ishikawa K, Fujita M, Sasaki Y, Mitamura S, Murao N, Funayama E, Yamamoto Y, Sasaki S
24th Workshop of the International Society for the Study of Vascular Anomalies (ISSVA), 10 May 2022, English, Poster presentation
10 May 2022 - 13 May 2022

〔Major achievements〕The financial impact of plastic surgeons in team surgery: A 10-year analysis of surgical revenue in a national university hospital in Japan　　　　　　　　　　　　　　　
Ishikawa K, Furukawa H, Oyama A, Funayama E, Hayashi T, Murao N, Shichinohe R, Maeda T, Sekido M, Yamamoto Y
American Society of Plastic Surgeons (ASPS) Plastic Surgery The Meeting 2016, 23 Sep. 2016, English, Poster presentation
Los Angeles, California

Percutaneous sclerotherapy for intramuscular venous malformations of the masticatory muscles　　　　　　　　　　　　　　　
Ishikawa K, Sasaki S, Furukawa H, Saito N, Nagao M, Ishiyama S, Iwasaki D, Fujita M, Sugii M, Takahashi H, Nozaki A, Yamatsu Y, Omote C, Funayama E, Oyama A, Yamamoto Y
The 16th Biennial Congress of the International Society of Craniofacial Surgery (ISCFS), 14 Sep. 2015, English, Poster presentation
Maihama

Evaluation of bone height in osseous mandibular reconstruction using free fibular grafts　　　　　　　　　　　　　　　
Ishikawa K, Hayashi T, Furukawa H, Shichinohe R, Oyama A, Funayama E, Murao N, Sekido M, Yamamoto Y
The Joint Meeting of 4th Congress of Asian Society of Head and Neck Oncology (ASHNO) and 39th Annual Meeting of Japan Society for Head and Neck Cancer (JSHNC), 03 Jun. 2015, English, Public symposium
Kobe

〔Major achievements〕Management of extensive peristomal skin ulcer associated with colon cancer: Usefulness of negative pressure wound therapy for skin graft fixation　　　　　　　　　　　　　　　
Ishikawa K, Minamimoto T, Mizuki T, Furukawa N, Terashima K, Sugii M
46th Annual Conference of the Wound, Ostomy and Continence Nurses Society, 21 Jun. 2014, English, Poster presentation
Nashville, Tennessee

〔Major achievements〕Preliminary experience with intraoperative near-infrared fluorescence imaging in percutaneous sclerotherapy of soft-tissue vascular malformations　　　　　　　　　　　　　　　
Ishikawa K, Sasaki S, Furukawa H, Nagao M, Iwasaki D, Saito N, Yamamoto Y
20th Workshop of the International Society for the Study of Vascular Anomalies (ISSVA), 02 Apr. 2014, English, Poster presentation
Melbourne, Australia

Integrated Cancer Diagnostics and Therapeutic Science II　　　　　　　　　　　　　　　
Graduate School of Medicine, Hokkaido University
Apr. 2024 - Present

Surgery (Plastic and Reconstructive Surgery)　　　　　　　　　　　　　　　
School of Dental Medicine, Hokkaido University
Oct. 2021 - Present

Plastic and Reconstructive Surgery　　　　　　　　　　　　　　　
School of Medicine, Hokkaido University
Apr. 2020 - Present

Plastic and Reconstructive Surgery　　　　　　　　　　　　　　　
Health Sciences University of Hokkaido
Apr. 2020 - Mar. 2022

May 2024 - Present
Japanese Society of Chemotherapy　　　　　　　　　　　　　　　

Apr. 2024 - Present
Japanese Society of Phlebology　　　　　　　　　　　　　　　

Jan. 2019 - Present
The Japanese Society of Lymphology　　　　　　　　　　　　　　　

Jun. 2018 - Present
International Society for the Study of Vascular Anomalies　　　　　　　　　　　　　　　

Sep. 2013 - Present
Japan Society for Laser Surgery and Medicine　　　　　　　　　　　　　　　

May 2013 - Present
Japanese Society for Surgery of the Hand　　　　　　　　　　　　　　　

Feb. 2013 - Present
Japanese Society of Pressure Ulcers　　　　　　　　　　　　　　　

Nov. 2011 - Present
Japan Society of Cranio-Maxillo-Facial Surgery　　　　　　　　　　　　　　　

Jul. 2011 - Present
Japanese Society for the Study of Vascular Anomalies　　　　　　　　　　　　　　　

Aug. 2010 - Present
Japanese Society for Burn Injuries　　　　　　　　　　　　　　　

Apr. 2010 - Present
Japan Society for Surgical Wound Care　　　　　　　　　　　　　　　

Mar. 2010 - Present
Japan Surgical Society　　　　　　　　　　　　　　　

Apr. 2008 - Present
Japan Society of Plastic and Reconstructive Surgery　　　　　　　　　　　　　　　

Cover Art, Annals of Plastic Surgery, Volume 92, Issue 6　　　　　　　　　　　　　　　
Ishikawa K, 01 Jun. 2024, [Others]

続発性リンパ浮腫における急性期重症化を防ぐ！レグネース１を標的とした新たな治療法
科学研究費助成事業
01 Apr. 2024 - 31 Mar. 2027
佐藤 千草, 舟山 恵美, 松田 識郁, 石川 耕資, 前田 拓
日本学術振興会, 基盤研究(C), 北海道大学, 24K12817

幹細胞によるM１/M２マクロファージ調整作用はリンパ浮腫線維化を抑制しうるか？
科学研究費助成事業
01 Apr. 2024 - 31 Mar. 2027
前田 拓, 石川 耕資, 山本 有平, 村尾 尚規
日本学術振興会, 基盤研究(C), 北海道大学, 24K12818

Restructuring of the lymphatic basin with HIF-1a stabilizers
Grants-in-Aid for Scientific Research
01 Apr. 2024 - 31 Mar. 2027
草島 英梨香, 石川 耕資, 前田 拓, 三浦 隆洋, 北條 正洋, 徐 東經
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 24K12841

SASP Regulation of Photoaging - Skin Rejuvenation by Stem Cell Exosomes
Grants-in-Aid for Scientific Research
01 Apr. 2023 - 31 Mar. 2026
高橋 周子, 山本 有平, 前田 拓, 石川 耕資
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 23K09073

Research Committee for Intractable Vascular Anomalies
Health, Labour and Welfare Sciences Research Grants
Apr. 2023 - Mar. 2026
秋田 定伯, 佐々木 了, 三村 秀文, 力久 直昭, 田中 純子, 小関 道夫, 森本 哲, 掛江 直子, 康 勝好, 木下 義晶, 神人 正寿, 藤野 明浩, 杠 俊介, 野村 正, 野崎 太希, 石川 耕資, 長濱 通子, 川上 善久, 岩科 裕己
The Ministry of Health, Labour and Welfare, Coinvestigator, 23810518

Genetic diagnosis of vascular malformations by liquid biopsy and its application to precision medicine
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Apr. 2023 - Mar. 2026
Ishikawa K, Yamamoto Y, Funayama E, Maeda T
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, 23K09072

Research Committee for Intractable Vascular Anomalies　　　　　　　　　　　　　　　
Health, Labour and Welfare Sciences Research Grants
Apr. 2020 - Mar. 2023
秋田 定伯, 佐々木 了, 三村 秀文, 力久直昭, 田中 純子, 小関 道夫, 森本 哲, 掛江 直子, 康勝好, 木下 義晶, 神人 正寿, 藤野 明浩, 杠 俊介, 野村 正, 野崎 太希, 石川 耕資, 長濱 通子
The Ministry of Health, Labour and Welfare, Coinvestigator, 20317609

Novel treatment of chronic wounds of the lower extremity with drugs activating the hypoxic response system　　　　　　　　　　　　　　　
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Apr. 2020 - Mar. 2023
Osawa M, Funayama E, Yamamoto Y, Maeda T, Ishikawa K, Hayashi T
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, 20K09841

Roles of the hypoxia response system in keloids: Normalization of local immunity and suppression of inflammation and fibrosis　　　　　　　　　　　　　　　
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Apr. 2020 - Mar. 2023
Murao N, Yamamoto Y, Hayashi T, Funayama E, Maeda T, Ishikawa K
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, 20K09858

Gene mutation analysis of PI3K pathway in patients with Klippel-Trenaunay syndrome　　　　　　　　　　　　　　　
Medical Research Support Project
Jan. 2021 - Jan. 2023
Kosuke Ishikawa
Japan Intractable Diseases (Nanbyo) Research Foundation, 臨床枠, Principal investigator

Breakthrough in Oncoplastic Skin Surgery
Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
Apr. 2020 - Mar. 2022
Ishikawa K
Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, Principal investigator, Competitive research funding, 20K18431

Potential abilities of visceral fat:circulation and immunity (hyper 2-way player)
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Apr. 2019 - Mar. 2022
Yamamoto Y, Hayashi T, Funayama E, Maeda T, Oyama A, Furukawa H, Ishikawa K
形成外科領域では以前からその豊富な血流と形態から複雑な欠損の再建材料として大網組織の移植を行ってきた。リンパ組織を多く含むことから免疫賦活効果が期待され、縦隔炎などの重症感染症に対する有用性が報告されてきた。リンパ浮腫に対する効果も報告され、循環系の改善効果についても示唆されている。
一方、これらの組織移植は、採取の侵襲が大きく、治療効果がその犠牲を上回る必要があるが、他の組織移植と比べ内臓脂肪組織中にリンパ組織を多く含み、他の組織移植にはない効果が期待される。未だ解明されていないこれらの組織移植の循環改善・免疫賦活効果を証明することで、組織移植として内臓脂肪が有する新たな可能性を模索する。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator, Competitive research funding, 19H03811

Development of antiangiogenic therapy targeting mTOR signaling pathway for the treatment of complicated vascular malformations
Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
Apr. 2018 - Mar. 2020
Ishikawa K
血管奇形は局所的な脈管形成異常により生じた先天性の病変で、我が国の疫学調査では42%が難治性であり、術後の再発予防を含めた新たな薬物療法の開発が望まれる。血管奇形の病変増大には、血流不均衡による局所的な低酸素状態に起因した血管新生の寄与などが考えられるが、その病態はいまだ解明されていない。治療法には切除や塞栓療法、硬化療法などがあるが、確立された薬物療法はない。近年、欧米で行われたラパマイシンを用いた難治性血管腫・血管奇形に対する第Ⅱ相臨床試験において、85%の症例で改善が認められ、新たな薬物療法として期待が高まっているが、血管奇形に関する基礎研究は少ないのが現状である。ラパマイシンは、その標的タンパク質mTORの阻害によって血管新生を抑制する作用があるとされる。血管奇形においてmTOR経路を中心とした血管新生が起きているのかを検討し、mTOR経路をターゲットにした新たな治療を開発することを本研究の目的とした。
北海道大学病院および斗南病院において、本研究に関する自主臨床研究を申請し、承認を得た。対象となる症例の既存パラフィン包埋組織標本を調査し、mTOR経路のタンパク質および各種血管新生マーカーに対する免疫組織化学染色を検討した。また、血管奇形の手術残余検体から血管内皮細胞の初代培養を行った。さらに、硬化療法後の静脈閉塞動物モデルとして、ラットを用いた硬化剤による大腿静脈閉塞モデル作製を試みた。
Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, Principal investigator, Competitive research funding, 18K16974

Development of functional lymph node transfer
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Apr. 2016 - Mar. 2019
Yamamoto Y, Oyama A, Funayama E, Hayashi T, Murao N, Osawa M, Maeda T, Ishikawa K, Furukawa H
Vascularized lymph node transfer is gaining popularity in the treatment of lymphedema. However, it is unclear whether vascularization of transferred lymph nodes contributes to functional improvement. This is the first experimental study to compare changes in the quantitative histology of transferred lymph nodes with or without vascularization in a mouse model of vascularized lymph node transfer. We found that vascularized lymph node transfer preserved more functional high endothelial venules within the transferred lymph node than did nonvascularized lymph node transfer.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator, Competitive research funding, 16H05491

リンパ浮腫・慢性創傷に対する低酸素応答システム活性化薬剤 による薬物療法の開発：動物実験からの考察
02 Mar. 2025
Lecturer
Lecture
令和６年度 厚生労働科学研究費補助金 難治性血管腫・脈管奇形・血管奇形・リンパ管奇形（リンパ管腫）・リンパ管腫症および関連疾患についての調査研究
市民公開講座（オンラインZoomウェビナー）
45539957

脈管奇形における遺伝子診断と治療への展開について
04 Feb. 2024
Lecturer
Lecture
令和５年度 厚生労働科学研究費補助金 難治性血管腫・脈管奇形・血管奇形・リンパ管奇形（リンパ管腫）・リンパ管腫症および関連疾患についての調査研究
市民公開講座（オンラインZoomウェビナー）
45539957