Okajima Takaharu

Faculty of Information Science and Technology Bioengineering and Bioinformatics BioengineeringProfessor
Education and Research Center for Mathematical and Data ScienceProfessor
Last Updated :2025/06/07

■Researcher basic information

Degree

  • Ph.D., Tokyo Institute of technology

Researchmap personal page

Research Keyword

  • 応用物理学一般
  • 生物物理学
  • バイオナノテクノロジー
  • Polymer Science
  • Biophysics
  • Bionanotechnology

Research Field

  • Life sciences, Biophysics
  • Nanotechnology/Materials, Applied physics - general
  • Natural sciences, Bio-, chemical, and soft-matter physics
  • Nanotechnology/Materials, Nanobioscience
  • Nanotechnology/Materials, Nanomaterials

Educational Organization

■Career

Career

  • Apr. 2019 - Present
    Hokkaido University, 大学院情報科学研究院, 教授
  • May 2013 - Mar. 2019
    Hokkaido University, Graduate School of Information Science and Technology
  • Apr. 2007 - Apr. 2013
    Hokkaido University, Graduate School of Information Science and Technology
  • Apr. 2003 - Mar. 2007
    Hokkaido University, Research Institute for Electronic Science, Nanotechnology Research Center
  • Apr. 1998 - Mar. 2003
    Tokyo Institute of Technology, Bioscience and Biotechnology, 助手
  • Oct. 1995 - Mar. 1998
    Tokyo Institute of Technology, School of Bioscience and Biotechnology, 助手

Educational Background

  • 1993, Tokyo Institute of Technology, Science of Engineering, 物理学専攻, Japan
  • 1991, Yokohama City University, 文理学部, 理科(物理学), Japan

■Research activity information

Papers

  • Mechanical properties of epithelial cells in domes investigated using atomic force microscopy
    K. Shigemura, K. Kuribayashi-Shigetomi, R. Tanaka, H. Yamasaki, T. Okajima
    Frontiers in Cell and Developmental Biology, 11, 1245296, Oct. 2023, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Atomic force microscopy for investigating cell and tissue mechanics as heterogeneous and hierarchical materials, Journal of Biomechanical Science and Engineering
    T. Okajima, K. Kuribayashi-Shigetomi
    Journal of Biomechanical Science and Engineering, Oct. 2023, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
    English, Scientific journal
  • Pathogenic Roles of Cardiac Fibroblasts in Pediatric Dilated Cardiomyopathy.
    Hirofumi Tsuru, Chika Yoshihara, Hidehiro Suginobe, Mizuki Matsumoto, Yoichiro Ishii, Jun Narita, Ryo Ishii, Renjie Wang, Atsuko Ueyama, Kazutoshi Ueda, Masaki Hirose, Kazuhisa Hashimoto, Hiroki Nagano, Ryosuke Tanaka, Takaharu Okajima, Keiichi Ozono, Hidekazu Ishida
    Journal of the American Heart Association, e029676, 22 Jun. 2023, [International Magazine]
    English, Scientific journal, Background Dilated cardiomyopathy (DCM) is a major cause of heart failure in children. Despite intensive genetic analyses, pathogenic gene variants have not been identified in most patients with DCM, which suggests that cardiomyocytes are not solely responsible for DCM. Cardiac fibroblasts (CFs) are the most abundant cell type in the heart. They have several roles in maintaining cardiac function; however, the pathological role of CFs in DCM remains unknown. Methods and Results Four primary cultured CF cell lines were established from pediatric patients with DCM and compared with 3 CF lines from healthy controls. There were no significant differences in cellular proliferation, adhesion, migration, apoptosis, or myofibroblast activation between DCM CFs compared with healthy CFs. Atomic force microscopy revealed that cellular stiffness, fluidity, and viscosity were not significantly changed in DCM CFs. However, when DCM CFs were cocultured with healthy cardiomyocytes, they deteriorated the contractile and diastolic functions of cardiomyocytes. RNA sequencing revealed markedly different comprehensive gene expression profiles in DCM CFs compared with healthy CFs. Several humoral factors and the extracellular matrix were significantly upregulated or downregulated in DCM CFs. The pathway analysis revealed that extracellular matrix receptor interactions, focal adhesion signaling, Hippo signaling, and transforming growth factor-β signaling pathways were significantly affected in DCM CFs. In contrast, single-cell RNA sequencing revealed that there was no specific subpopulation in the DCM CFs that contributed to the alterations in gene expression. Conclusions Although cellular physiological behavior was not altered in DCM CFs, they deteriorated the contractile and diastolic functions of healthy cardiomyocytes through humoral factors and direct cell-cell contact.
  • Atomic force microscopy identifies the alteration of rheological properties of the cardiac fibroblasts in idiopathic restrictive cardiomyopathy.
    Mizuki Matsumoto, Hirofumi Tsuru, Hidehiro Suginobe, Jun Narita, Ryo Ishii, Masaki Hirose, Kazuhisa Hashimoto, Renjie Wang, Chika Yoshihara, Atsuko Ueyama, Ryosuke Tanaka, Keiichi Ozono, Takaharu Okajima, Hidekazu Ishida
    PloS one, 17, 9, e0275296, Sep. 2022, [Corresponding author], [International Magazine]
    English, Scientific journal, Restrictive cardiomyopathy (RCM) is a rare disease characterized by increased ventricular stiffness and preserved ventricular contraction. Various sarcomere gene variants are known to cause RCM; however, more than a half of patients do not harbor such pathogenic variants. We recently demonstrated that cardiac fibroblasts (CFs) play important roles in inhibiting the diastolic function of cardiomyocytes via humoral factors and direct cell-cell contact regardless of sarcomere gene mutations. However, the mechanical properties of CFs that are crucial for intercellular communication and the cardiomyocyte microenvironment remain less understood. In this study, we evaluated the rheological properties of CFs derived from pediatric patients with RCM and healthy control CFs via atomic force microscopy. Then, we estimated the cellular modulus scale factor related to the cell stiffness, fluidity, and Newtonian viscosity of single cells based on the single power-law rheology model and analyzed the comprehensive gene expression profiles via RNA-sequencing. RCM-derived CFs showed significantly higher stiffness and viscosity and lower fluidity compared to healthy control CFs. Furthermore, RNA-sequencing revealed that the signaling pathways associated with cytoskeleton elements were affected in RCM CFs; specifically, cytoskeletal actin-associated genes (ACTN1, ACTA2, and PALLD) were highly expressed in RCM CFs, whereas several tubulin genes (TUBB3, TUBB, TUBA1C, and TUBA1B) were down-regulated. These results implies that the signaling pathways associated with cytoskeletal elements alter the rheological properties of RCM CFs, particularly those related to CF-cardiomyocyte interactions, thereby leading to diastolic cardiac dysfunction in RCM.
  • Pharmacological Alteration of Cellular Mechanical Properties in Pulmonary Arterial Smooth Muscle Cells of Idiopathic Pulmonary Arterial Hypertension
    Shinichi Katsuragi, Nao Tatsumi, Mizuki Matsumoto, Jun Narita, Ryo Ishii, Hidehiro Suginobe, Hirofumi Tsuru, Renjie Wang, Shigetoyo Kogaki, Ryosuke Tanaka, Keiichi Ozono, Takaharu Okajima, Hidekazu Ishida
    Cardiology Research, 12, 4, 231, 237, Elmer Press, Inc., Aug. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal, Background: Idiopathic pulmonary arterial hypertension (IPAH) is a progressive disease caused by vascular remodeling of the pulmonary arteries with elevated pulmonary vascular resistance. Recently, various pulmonary vasodilator drugs have become available in the clinical field, and have dramatically ameliorated the prognosis of IPAH. However, little is known about how the mechanical properties of pulmonary arterial smooth muscle cells (PASMCs) are altered under drug supplementation. Methods: Atomic force microscopy (AFM) was used to investigate the mechanical properties of PASMCs derived from a patient with IPAH (PAH-PASMCs) and a healthy control (N-PASMCs) which received the supplementation of clinically used drugs for IPAH: sildenafil, macitentan, and riociguat. Results: PASMCs derived from PAH-PASMCs were stiffer than those derived from N-PASMCs. With sildenafil treatment, the apparent Young's modulus (E 0) of cells significantly decreased in PAH-PASMCs but remained unchanged in N-PASMCs. The decrease in E 0 of PAH-PASMCs was also observed in macitentan and riociguat treatment. The stress relaxation AFM revealed that the decrease in E 0 of PAH-PASMCs resulted from a decrease in the cell elastic modulus and/or increase in cell fluidity. The combination treatment of macitentan and riociguat showed an additive effect on cell mechanical properties, implying that this clinically accepted combination therapy for IPAH influences the intracellular mechanical components. Conclusions: Pulmonary vasodilator drugs affect the mechanical properties of PAH-PASMCs, and there exists a mechanical effect of combination treatment on PAH-PASMCs.
  • Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo
    Y. Fujii, W. C. Koizumi, T. Imai, M. Yokobori, T. Matsuo, K. Oka, K. Hotta, T. Okajima
    Communications Biology, 4, 1, 341, 341, Springer Science and Business Media LLC, 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, AbstractDuring the developmental processes of embryos, cells undergo massive deformation and division that are regulated by mechanical cues. However, little is known about how embryonic cells change their mechanical properties during different cleavage stages. Here, using atomic force microscopy, we investigated the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. In both animal and vegetal hemispheres, we observed a Rho kinase (ROCK)-independent cell stiffening that the cell stiffness exhibited a remarkable increase at the timing of cell division where cortical actin filaments were organized. Furthermore, in the vegetal hemisphere, we observed another mechanical behaviour, i.e., a ROCK-associated cell stiffening, which was retained even after cell division or occurred without division and propagated sequentially toward adjacent cells, displaying a characteristic cell-to-cell mechanical variation. The results indicate that the mechanical properties of embryonic cells are regulated at the single cell level in different germ layers.
  • Measuring viscoelasticity of soft biological samples using atomic force microscopy               
    Yuri M. Efremov, Takaharu Okajima, Arvind Raman
    Soft Matter, 16, 64, 81, 2020, [Peer-reviewed]
    English, Scientific journal
  • Stiffness of brewers’ yeast under ethanol stress investigated by atomic force microscopy               
    K. Toyota, R. Tanaka, T. Okajima
    Japanese Journal of Applied Physics, 59, SN1005, 2020, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Relationship between rheological properties and actin filaments of single cells investigated by atomic force microscopy               
    R. Tanaka, M. Sawano, Y. Fujii, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima*
    Japanese Journal of Applied Physics, 59, SN1010, 2020, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Spontaneous Spatial Correlation of Elastic Modulus in Jammed Epithelial Monolayers Observed by AFM.
    Yuki Fujii, Yuki Ochi, Masahiro Tuchiya, Mihoko Kajita, Yasuyuki Fujita, Yukitaka Ishimoto, Takaharu Okajima
    Biophysical journal, 116, 6, 1152, 1158, 19 Mar. 2019, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, For isolated single cells on a substrate, the intracellular stiffness, which is often measured as the Young's modulus, E, by atomic force microscopy (AFM), depends on the substrate rigidity. However, little is known about how the E of cells is influenced by the surrounding cells in a cell population system in which cells physically and tightly contact adjacent cells. In this study, we investigated the spatial heterogeneities of E in a jammed epithelial monolayer in which cell migration was highly inhibited, allowing us to precisely measure the spatial distribution of E in large-scale regions by AFM. The AFM measurements showed that E can be characterized using two spatial correlation lengths: the shorter correlation length, lS, is within the single cell size, whereas the longer correlation length, lL, is longer than the distance between adjacent cells and corresponds to the intercellular correlation of E. We found that lL decreased significantly when the actin filaments were disrupted or calcium ions were chelated using chemical treatments, and the decreased lL recovered to the value in the control condition after the treatments were washed out. Moreover, we found that lL decreased significantly when E-cadherin was knocked down. These results indicate that the observed long-range correlation of E is not fixed within the jammed state but inherently arises from the formation of a large-scale actin filament structure via E-cadherin-dependent cell-cell junctions.
  • Cricket tympanal organ revisited: morphology, development, and possible functions of the adult-specific chitin core beneath the anterior tympanal membrane
    H. Nishino, M. Domae, T. Takanashi, T. Okajima
    Cell and Tissue Research, 377, 2, 1, 22, Springer Science and Business Media LLC, 2019, [Peer-reviewed]
    English, Scientific journal
  • Calibrating the Young’s modulus of soft materials with surface tilt angle measured by atomic force microscopy               
    Y. Fujii, T. Okajima
    AIP Advances, 9, 015028, 2019, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin
    M. Tanaka, Y. Fujii, K. Hirano, T. Higaki, A. Nagasaki, R. Ishikawa, T. Okajima, K. Katoh
    Genes to Cells, 24, 3, 202, 213, 2019, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Fascin, an actin-bundling protein, is present in the filopodia and lamellipodia of growth cones. However, few studies have examined lamellipodial fascin because it is difficult to observe. In this study, we evaluated lamellipodial fascin. We visualized the actin meshwork of lamellipodia in live growth cones by super-resolution microscopy. Fascin was colocalized with the actin meshwork in lamellipodia. Ser39 of fascin is a well-known phosphorylation site that controls the binding of fascin to actin filaments. Fluorescence recovery after photobleaching experiments with confocal microscopy showed that binding of fascin was controlled by phosphorylation of Ser39 in lamellipodia. Moreover, TPA, an agonist of protein kinase C, induced phosphorylation of fascin and dissociation from actin filaments in lamellipodia. Time series images showed that dissociation of fascin from the actin meshwork was induced by TPA. As fascin dissociated from actin filaments, the orientation of the actin filaments became parallel to the leading edge. The angle of actin filaments against the leading edge was changed from 73° to 15°. This decreased the elasticity of the lamellipodia by 40%, as measured by atomic force microscopy. These data suggest that actin bundles made by fascin contribute to elasticity of the growth cone.
  • Visualising the dynamics of live pancreatic microtumours self-organised through cell-in-cell invasion
    Y. Miyatake, K. Kuribayashi-Shigetomi, Y. Ohta, S. Ikeshita, A. Subagyo, K. Sueoka, A. Kakugo, M. Amano, T. Takahashi, T. Okajima, M. Kasahara
    Scientific Reports, 8, 1, 14054, Springer Science and Business Media LLC, 2018, [Peer-reviewed]
    English, Scientific journal
  • Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructure
    Q. He, T. Okajima, H. Onoe, A. Subagyo, K. Sueoka, K. Kuribayashi-Shigetomi
    Scientific Reports, 8, 1, 4556, Springer Science and Business Media LLC, 2018, [Peer-reviewed]
    English, Scientific journal
  • Regulation of Neuritogenesis in Hippocampal Neurons using Stiffness of Extracellular Microenvironment
    A. Tanaka, Y. Fujii, N. Kasai, T. Okajima, H. Nakashima
    PLOS ONE, 13, 2, e019192, 2018, [Peer-reviewed], [International Magazine]
    English, Scientific journal, The mechanosensitivity of neurons in the central nervous system (CNS) is an interesting issue as regards understanding neuronal development and designing compliant materials as neural interfaces between neurons and external devices for treating CNS injuries and disorders. Although neurite initiation from a cell body is known to be the first step towards forming a functional nervous network during development or regeneration, less is known about how the mechanical properties of the extracellular microenvironment affect neuritogenesis. Here, we investigated the filamentous actin (F-actin) cytoskeletal structures of neurons, which are a key factor in neuritogenesis, on gel substrates with a stiffness-controlled substrate, to reveal the relationship between substrate stiffness and neuritogenesis. We found that neuritogenesis was significantly suppressed on a gel substrate with an elastic modulus higher than the stiffness of in vivo brain. Fluorescent images of the F-actin cytoskeletal structures showed that the F-actin organization depended on the substrate stiffness. Circumferential actin meshworks and arcs were formed at the edge of the cell body on the stiff gel substrates unlike with soft substrates. The suppression of F-actin cytoskeleton formation improved neuritogenesis. The results indicate that the organization of neuronal F-actin cytoskeletons is strongly regulated by the mechanical properties of the surrounding environment, and the mechanically-induced F-actin cytoskeletons regulate neuritogenesis.
  • Temporal Variation in Single-Cell Power-Law Rheology Spans the Ensemble Variation of Cell Population
    PingGen Cai, Ryosuke Takahashi, Kaori Kuribayashi-Shigetomi, Agus Subagyo, Kazuhisa Sueoka, John M. Maloney, Krystyn J. Van Vliet, Takaharu Okajima
    Biophysical Journal, 113, 3, 671, 678, Aug. 2017, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Mechanical properties of paraformaldehyde‑treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy               
    S.‐O. Kim, J. Kim, T. Okajima, N.‐J. Cho
    Nano Convergence, 4, 5, 2017, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Comparison between power-law rheological parameters of living cells in frequency and time domains measured by atomic force microscopy
    Ryosuke Takahashi, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS, 55, 8, 08NB22, Aug. 2016, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Simple and rapid formation of 3D co-culture cell laden microstructures by using cell origami technique
    Q. He, T. Okajima, K. K. Shigetomi
    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 3, 4, 2016
    International conference proceedings
  • Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy
    Ryosuke Takahashi, Takaharu Okajima
    APPLIED PHYSICS LETTERS, 107, 17, Oct. 2015, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Time-lapse imaging of morphological changes in a single neuron during the early stages of apoptosis using scanning ion conductance microscopy
    Aya Tanaka, Ryosuke Tanaka, Nahoko Kasai, Shingo Tsukada, Takaharu Okajima, Koji Sumitomo
    JOURNAL OF STRUCTURAL BIOLOGY, 191, 1, 32, 38, Jul. 2015, [Peer-reviewed]
    English, Scientific journal
  • Precision of cell-to-cell variation in power-law rheology characterized by atomic force microscopy
    PingGen Cai, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS, 54, 3, Mar. 2015, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Atomic force microscopy for mapping mechanical property of the whole cell assembly
    Ryosuke Tanaka, Yuki Fujii, Junpei Kikkawa, Kaori Kuribayashi-Shigetomi, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima
    2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014, Institute of Electrical and Electronics Engineers Inc., 09 Jan. 2015
    English, International conference proceedings
  • Atomic Force Microscopy: Imaging and Rheology of Living Cells               
    Takaharu Okajima
    Nano/Micro Science and Technology in Biorheology, 2015, [Invited]
  • High-throughput Measurements of Single Cell Rheology by Atomic Force Microscopy               
    High-throughput Measurements of Single Cell Rheology by, Atomic Force Microscopy, Kaori Kuribayashi-Shigetomi, Ryosuke Takahashi, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima
    Hyper Bio Assembler for 3D Cellular Systems, 57, 67, 2015, [Last author, Corresponding author]
    English
  • Filamin acts as a key regulator in epithelial defence against transformed cells
    Mihoko Kajita, Kaoru Sugimura, Atsuko Ohoka, Jemima Burden, Hitomi Suganuma, Masaya Ikegawa, Takashi Shimada, Tetsuya Kitamura, Masanobu Shindoh, Susumu Ishikawa, Sayaka Yamamoto, Sayaka Saitoh, Yuta Yako, Ryosuke Takahashi, Takaharu Okajima, Junichi Kikuta, Yumiko Maijima, Masaru Ishii, Masazumi Tada, Yasuyuki Fujita
    NATURE COMMUNICATIONS, 5, Jul. 2014, [Peer-reviewed]
    English, Scientific journal
  • Atomic force microscopy measurements of mechanical properties of single cells patterned by microcontact printing
    Ryosuke Takahashi, Satoshi Ichikawa, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima
    ADVANCED ROBOTICS, 28, 7, 449, 455, Apr. 2014, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Temporal Change in Complex Shear Modulus of Cells: An Atomic Force Microscopy Study
    PingGen Cai, Ryosuke Takahashi, Kaori Kuribayashi-Shigetomi, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima
    2014 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS), 1, 3, 2014, [Peer-reviewed]
    English, International conference proceedings
  • Quantitative Rheological Measurements of Confluent Cell Using Atomic Force Microscopy
    Ryosuke Takahashi, Kaori Kuribayashi-Shigetomi, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima
    2014 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS), 1, 3, 2014, [Peer-reviewed]
    English, International conference proceedings
  • Quantifying cell-to-cell variation in power-law rheology
    Pinggen Cai, Yusuke Mizutani, Masahiro Tsuchiya, John M. Maloney, Ben Fabry, Krystyn J. Van Vliet, Takaharu Okajima
    Biophysical Journal, 105, 5, 1093, 1102, 03 Sep. 2013, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • Nanoscale fluctuations on epithelial cell surfaces investigated by scanning ion conductance microscopy
    Yusuke Mizutani, Myung-Hoon Choi, Sang-Joon Cho, Takaharu Okajima
    APPLIED PHYSICS LETTERS, 102, 17, Apr. 2013, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal
  • High-throughput measurements of cell mechanics using atomic force microscopy with micro-patterned substrates.
    Ryosuke Takahashi, Kaori Kuribayashi-Shigetomi, Masahiro Tsuchiya, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima
    International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013, Nagoya, Japan, November 10-13, 2013, 1, 3, 2013, [Peer-reviewed]
    International conference proceedings
  • 原子間力顕微鏡を用いた細胞形状を制御した単一細胞のレオロジー空間測定
    市川 論, 水谷 祐輔, 高橋 亮輔, 河原 剛一, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2012.1, 2550, 2550, 公益社団法人 応用物理学会, 29 Feb. 2012
    Japanese
  • イオンコンダクタンス顕微鏡による細胞膜揺らぎ測定
    水谷 祐輔, 蔡 萍根, 土屋 雅博, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2012.1, 1595, 1595, 公益社団法人 応用物理学会, 29 Feb. 2012
    Japanese
  • Effect of isoproterenol on local contractile behaviors of rat cardiomyocytes measured by atomic force microscopy
    Yusuke Mizutani, Koichi Kawahara, Takaharu Okajima
    Current Pharmaceutical Biotechnology, 13, 14, 2599, 2603, Bentham Science Publishers B.V., 2012, [Peer-reviewed]
    English, Scientific journal
  • Atomic force microscopy for the examination of single cell rheology
    Takaharu Okajima
    Current Pharmaceutical Biotechnology, 13, 14, 2623, 2631, Bentham Science Publishers B.V., 2012, [Peer-reviewed]
    English
  • Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy
    Akinori Okada, Yusuke Mizutani, Agus Subagyo, Hirotaka Hosoi, Motonori Nakamura, Kazuhisa Sueoka, Koichi Kawahara, Takaharu Okajima
    APPLIED PHYSICS LETTERS, 99, 26, 263703, Dec. 2011, [Peer-reviewed]
    English, Scientific journal
  • Negative-feedback regulation of ATP release: ATP release from cardiomyocytes is strictly regulated during ischemia
    Kunugi, Satohiko, Iwabuchi, Sadahiro, Matsuyama, Daisuke, Okajima, Takaharu, Kawahara, Koichi
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 416, 3-4, 409, 415, Dec. 2011, [Peer-reviewed]
    English, Scientific journal
  • AFMによる単一細胞レオロジーの個体差の精密解析
    蔡 萍根, 水谷 祐輔, 土屋 雅博, 河原 剛一, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2011.2, 1077, 1077, 公益社団法人 応用物理学会, 16 Aug. 2011
    Japanese
  • Rheological Properties of Growth-Arrested Fibroblast Cells under Serum Starvation Measured by Atomic Force Microscopy
    Atsushi Miyaoka, Yusuke Mizutani, Masahiro Tsuchiya, Koichi Kawahara, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS, 50, 8, 08LB16, Aug. 2011, [Peer-reviewed]
    English, Scientific journal
  • AFMによる細胞内ネットワーク構造変化に対する細胞粘弾性の研究
    蔡 萍根, 水谷 祐輔, 土屋 雅博, 河原 剛一, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2011.1, 1637, 1637, 公益社団法人 応用物理学会, 09 Mar. 2011
    Japanese
  • AFMによる不死化過程における細胞粘弾性の細胞数分布解析
    水谷 祐輔, 蔡 萍根, 土屋 雅博, 河原 剛一, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2011.1, 1636, 1636, 公益社団法人 応用物理学会, 09 Mar. 2011
    Japanese
  • 原子間力顕微鏡を用いた物理刺激による細胞カルシウム応答測定
    佐藤 淳平, 水谷 祐輔, 河原 剛一, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2011.1, 2723, 2723, 公益社団法人 応用物理学会, 09 Mar. 2011
    Japanese
  • Artificial polymeric receptors on the cell surface promote the efficient cellular uptake of quantum dots
    Kenichi Niikura, Katsuyuki Nambara, Takaharu Okajima, Ryosuke Kamitani, Shin Aoki, Yasutaka Matsuo, Kuniharu Ijiro
    ORGANIC & BIOMOLECULAR CHEMISTRY, 9, 16, 5787, 5792, 2011, [Peer-reviewed]
    English, Scientific journal
  • Influence of Hydrophobic Structures on the Plasma Membrane Permeability of Lipidlike Molecules
    Kenichi Niikura, Katsuyuki Nambara, Takaharu Okajima, Yasutaka Matsuo, Kuniharu Ijiro
    LANGMUIR, 26, 12, 9170, 9175, Jun. 2010, [Peer-reviewed]
    English, Scientific journal
  • AFMによる形質転換過程における線維芽細胞の複素弾性率の解析
    水谷 祐輔, 蔡 萍根, 宮岡 敦史, 土屋 雅博, 春菜 ゆかり, 河原 剛一, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2010.1, 2674, 2674, 公益社団法人 応用物理学会, 03 Mar. 2010
    Japanese
  • AFM による多数細胞レオロジーの普遍性と細胞個性の評価
    蔡 萍根, 水谷 祐輔, 宮岡 敦史, 土屋 雅博, 河原 剛一, 岡嶋 孝治
    応用物理学会学術講演会講演予稿集, 2010.1, 1526, 1526, 公益社団法人 応用物理学会, 03 Mar. 2010
    Japanese
  • Statistics of Single Cell Mechanics Investigated by Atomic Force Microscopy               
    Hiratsuka, Y. Mizutani, P.G. Cai, M. Tsuchiya, H. Tokumoto, K. Kawahara, T. Okajima
    MRS Spring Meeting Symposium U proceedings, 9999, 2010, [Peer-reviewed]
  • Power-Law Stress and Creep Relaxations of Single Cells Measured by Colloidal Probe Atomic Force Microscopy
    Shinichiro Hiratsuka, Yusuke Mizutani, Akitoshi Toda, Norichika Fukushima, Koichi Kawahara, Hiroshi Tokumoto, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS, 48, 8, 08JB14-4, Aug. 2009, [Peer-reviewed]
    English, Scientific journal
  • The number distribution of complex shear modulus of single cells measured by atomic force microscopy
    Shinichiro Hiratsuka, Yusuke Mizutani, Masahiro Tsuchiya, Koichi Kawahara, Hiroshi Tokumoto, Takaharu Okajima
    ULTRAMICROSCOPY, 109, 8, 937, 941, Jul. 2009, [Peer-reviewed]
    English, Scientific journal
  • Design of Cell-Surface-Retained Polymers for Artificial Ligand Display
    Ryosuke Kamitani, Kenichi Niikura, Takaharu Okajima, Yasutaka Matsuo, Kuniharu Ijiro
    CHEMBIOCHEM, 10, 2, 230, 233, Jan. 2009, [Peer-reviewed]
    English, Scientific journal
  • Localized yielding around crack tips of double-network gels(a)
    Yoshimi Tanaka, Yasunori Kawauchi, Takayuki Kurokawa, Hidemitsu Furukawa, Takaharu Okajima, Jian Ping Gong
    MACROMOLECULAR RAPID COMMUNICATIONS, 29, 18, 1514, 1520, Sep. 2008, [Peer-reviewed]
    English, Scientific journal
  • Elasticity of living cells on a microarray during the early stages of adhesion measured by atomic force microscopy
    Yusuke Mizutani, Masahiro Tsuchiya, Shinichiro Hiratsuka, Koichi Kawahara, Hiroshi Tokumot, Takaharu Okajima
    JAPANESE JOURNAL OF APPLIED PHYSICS, 47, 7, 6177, 6180, Jul. 2008, [Peer-reviewed]
    English, Scientific journal
  • Affinity of functional groups for membrane surfaces: Implications for physically irreversible fouling
    Hiroshi Yamamura, Katsuki Kimura, Takaharu Okajima, Hiroshi Tokumoto, Yoshimasa Watanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY, 42, 14, 5310, 5315, Jul. 2008, [Peer-reviewed]
    English, Scientific journal
  • Observation of the three-dimensional structure of actin bundles formed with polycations
    Kazuhiro Shikinaka, Hyuckjoon Kwon, Akira Kakugo, Hidemitsu Furukawa, Yoshihito Sada, Jian Ping Gong, Yoshitaka Aoyama, Hideo Nishioka, Hiroshi Jinnai, Takaharu Okajima
    BIOMACROMOLECULES, 9, 2, 537, 542, Feb. 2008, [Peer-reviewed]
    English, Scientific journal
  • Surface Mechanics of a Large Number of Cells Measured by AFM
    Mizutani Yusuke, Hiratsuka Shinichiro, Tsuchiya Masahiro, Kawahara Koichi, Tokumoto Hiroshi, Okajima Takaharu
    Abstract of annual meeting of the Surface Science of Japan, 28, 185, 185, The Japan Society of Vacuum and Surface Science, 2008
    Japanese, 原子間力顕微鏡(AFM)による細胞表面力学計測は、現在まで多数の研究がなされおり,1個または数個の細胞を用いた力学計測がなされてきた。一方で、細胞力学をより詳細に定量化するためには、多数の細胞を測定し,統計的な評価をすることが望まれる.本研究では,マイクロアレイ技術をAFM測定に利用して,多数の細胞の力学特性を高速に計測する方法を開発し、それを用いた細胞力学測定を行った.
  • Nanorheology of living cells investigated by atomic force microscopy               
    T. Okajima, H. Tokumoto
    Journal of the society of rheology, 36, 81, 86, 2008, [Lead author]
    Japanese
  • Stress relaxation measurement of fibroblast cells with atomic force microscopy
    Takaharu Okajima, Masaru Tanaka, Shusaku Tsukiyama, Tsubasa Kadowaki, Sadaaki Yamamoto, Masatsugu Shimomura, Hiroshi Tokumot
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 46, 8B, 5552, 5555, Aug. 2007, [Peer-reviewed]
    English, Scientific journal
  • Actin network formation by unidirectional polycation diffusion
    Hyuck Joon Kwon, Akira Kakugo, Takehiro Ura, Takaharu Okajima, Yoshimi Tanaka, Hidemitsu Furukawa, Yoshihito Osada, Jian Ping Gong
    LANGMUIR, 23, 11, 6257, 6262, May 2007, [Peer-reviewed]
    English, Scientific journal
  • Force spectroscopy on single polymer incorporated into polymer gels
    Takaharu Okajima, Xiang-Ming Tao, Hiroaki Azehara, Hiroshi Tokumoto
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 7, 3, 790, 795, Mar. 2007, [Peer-reviewed]
    English, Scientific journal
  • DNA microstructure based on self-assembly of 4-sticky-end holiday junctions in aqueous solution
    Hiroaki Kii, Takuya Takagi, Akira Sasaki, Takaharu Okajima, Masataka Kinjo
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 7, 3, 726, 729, Mar. 2007, [Peer-reviewed]
    English, Scientific journal
  • Stress relaxation of HepG2 cells measured by atomic force microscopy
    T. Okajima, M. Tanaka, S. Tsukiyama, T. Kadowaki, S. Yamamoto, M. Shimomura, H. Tokumoto
    NANOTECHNOLOGY, 18, 8, 084010, Feb. 2007, [Peer-reviewed]
    English, Scientific journal
  • Nano-dynamics of soft-matter surfaces investigated with an AFM noise analysis               
    Takaharu Okajima, Tsubasa Kadowaki, Xiang-Ming Tao, Hiroshi Tokumoto
    Polymer Preprints, Japan, 55, 2, 3091, 3092, 2006
    Japanese, International conference proceedings
  • Nano-dynamics of living cell surface by AFM               
    Takaharu Okajima, Masaru Tanaka, Shusaku Tsukiyama, Sadaaki Yamamoto, Masatsugu Shimomura, Hiroshi Tokumoto
    Polymer Preprints, Japan, 55, 1, 2109, 2006
    Japanese, International conference proceedings
  • Quantitative measurements of spatial correlation of polymer gel surfaces with AFM               
    Tsubasa Kadowaki, Takaharu Okajima, Hiroshi Tokumoto
    Polymer Preprints, Japan, 55, 1, 1030, 2006
    Japanese, International conference proceedings
  • Nano-measuring living cell surface by AFM               
    Takaharu Okajima, Masaru Tanaka, Katsuhiro Ishii, Hiroshi Tokumoto
    Polymer Preprints, Japan, 54, 1, 2376, 2005
    Japanese, International conference proceedings
  • Dynamics of a partially stretched protein molecule studied using an atomic force microscope               
    Takaharu Okajima, Hideo Arakawa, Mohammad, Taufiq Alam, Hiroshi Sekiguchi, Atsushi Ikai
    Biophysical Chemistry, 107, 51, 61, 2004, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • Frequency shift feedback imaging in liquid for biological molecules               
    Hiroshi Sekiguchi, Takaharu Okajima, Hideo Arakawa, Sumihiro Maeda, Akihiko Takashima, Atsushi Ikai
    Applied Surface Science, 210, 61, 67, 2003, [Peer-reviewed]
    English
  • Nano-mechanical methods in biochemistry using atomic force microscopy               
    Ikai, A, Afrin, R, Sekiguchi, H, Okajima, T, Alam, M.T, Nishida, S
    Current Protein and Peptide Science, 4, 2003, [Peer-reviewed]
    English
  • Self-oscillation technique for AFM in liquids               
    Takaharu Okajima, Hiroshi Sekiguchi, Hideo Arakawa, Atsushi Ikai
    Applied Surface Science, 210, 68, 72, 2003, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • Non-destructive force measurement in liquid using atomic force microscope               
    Hiroshi Sekiguchi, Hideo Arakawa, Takaharu Okajima, Atsushi Ikai
    Applied Surface Science, 188, 489, 2002
    English
  • Volume Phase Transition in Neutral Copolymer Gels               
    Takaharu Okajima, Shunsuke Hirotsu
    Transactions of the Materials Research Society(!{ Japan, 2001, [Peer-reviewed]
    English
  • Use of AFM for imaging and measurement of the mechanical properties of light-convertible organelles in plants               
    Takafumi Yamada, Hideo Arakawa, Takaharu Okajima, Takayoshi Shimada, Atsushi Ikai
    Ultramicroscopy, 91, 261, 268, 2001, [Peer-reviewed]
    English
  • Discontinuous Crossover between Fast and Slow Kinetics at the Volume Phase Transition in Poly-N-isopropylacrylamide Gels.
    Okajima Takaharu, Harada Ichiro, Nishio Kazufumi, Hirotsu Shunsuke
    Japanese Journal of Applied Physics, 39, 8, L875, L877, The Japan Society of Applied Physics, 2000
    English, The kinetics of the volume phase transition in N-isopropylacrylamide gels has been studied as a function of crosslinker concentration. The shrinking kinetics at the transition is extremely slow in gels with a standard composition which have been used widely in various experiments. A discontinuous crossover from slow to much faster kinetics were observed in both high- and low-crosslinker-concentration regions, where the characteristic time of the shrinking process changes abruptly by two to four orders of magnitude with a minute change of the crosslinker concentration. In spite of these marked changes in the kinetics, no anomaly was observed in the degree of equilibrium swelling in these regions. A mechanism leading to sudden changes in kinetics is discussed in terms of an inhomogeneous network structure which is dependent on crosslink density.
  • Study of shear force between glass microprobe and mica surface under controlled humidity               
    T Okajima, S Hirotsu
    Applied Physics Letters, 71, 545, 547, 1997, [Peer-reviewed], [Lead author, Corresponding author]
    English
  • Brillouin scattering study of the volume phase transition in poly-N-isopropylacrylamide gels
    Shunsuke Hirotsu, Ikuo Yamamoto, Atsushi Matsuo, Takaharu Okajima, Hidemitsu Furukawa, Tatsuyuki Yamamoto
    Journal of the Physical Society of Japan, 64, 8, 2898, 2907, Physical Society of Japan, 1995
    English, Scientific journal
  • Change in molecular orientation during thin film growth by evaporation of copolymer of vinylidene fluoride (80%) and tetrafluoroethylene (20%)               
    Takaharu Okajima, Kunisuke Maki
    Japanese Journal of Applied Physics, 30, L2055, 1991, [Peer-reviewed]
    English

Other Activities and Achievements

Books and other publications

  • 原子間力顕微鏡による発生胚皮質領域の力学測定               
    岡嶋孝治
    膜、47、269-272(2022)., Aug. 2022
  • 原子間力顕微鏡:多細胞系の力学物性計測の現状               
    岡嶋孝治
    生物物理(解説) 62、159-164(2022), Jan. 2022
  • ナノ計測 : 電子線・光・プローブ技術を用いたナノ・バイオ材料の探索と評価
    重川, 秀実, 吉村, 雅満, 目良, 裕, 岡嶋, 孝治
    近代科学社, Aug. 2020, 9784764950290, x, 179p, 図版 [4]p, Japanese
  • 原子間力顕微鏡法:細胞表面近傍のナノメカニクス               
    藤井裕紀, 岡嶋孝治
    表面と真空、63、437-440 (2020), 2020
  • AFMによる多細胞系の力学測定               
    藤井裕紀, 岡嶋孝治
    高分子、68、656-657, Dec. 2019, [Contributor]
  • AFMを用いた1細胞・細胞集団の力学物性計測               
    藤井裕紀, 岡嶋孝治
    生体の科学(特集:メカノバイオロジー)vol.70, No.4, Jul. 2019, [Contributor]
  • 細胞ナノレオロジー:原子間力顕微鏡法               
    岡嶋 孝治
    月刊ソフトマター, Sep. 2018, [Contributor]
  • 走査型プローブ顕微鏡               
    淺川雅, 岡嶋孝治, 大西洋
    日本分析化学会編、分析化学実技シリーズ機器分析編15, 2017, [Joint work]
  • 原子間力顕微鏡による1細胞レオロジーの定量計測─細胞の個性とエルゴード性               
    岡嶋 孝治
    応用物理学会誌、86、1052-1056, 2017, [Single work]
  • Nanorheology of Living Cells               
    T. Okajima, The World of Nano-Biomechanics (Chapter 13, A. Ikai)
    Elsevier, 2016, [Contributor]
  • 原子間力顕微鏡を用いた細胞の力学特性計測               
    岡嶋孝治, 高橋亮輔, 細胞の特性計測・操作と応用(組織工学ライブラリ-マイクロロボティクスとバイオの融合- 1)(第1章・第3節)
    コロナ社, 2016, [Contributor]
  • Atomic Force Microscopy: Imaging and Rheology of Living Cells               
    Takaharu Okajima
    Nano/Micro Science and Technology in Biorheology: Principles, Methods, and Applications Chapter 15 (387-414), Springer, (2015), 2015, [Joint work]
  • 原子間力顕微鏡を用いた細胞レオロジー特性の計測               
    Okajima, Takaharu
    三次元ティッシュエンジニアリング~細胞の培養・操作・組織化から品質管理、脱細胞化まで~(第1章・第3節)、(株式会社NTS) (2015), 2015, [Joint work]
  • イオンコンダクタンス顕微鏡:細胞膜表面の揺らぎを計測する               
    水谷祐輔, 田中良昌, 田中あや, 住友弘二, 岡嶋孝治
    光学-細胞機能に迫る非染色非破壊イメージング技術、44, (6月号)(2015), 2015, [Joint work]
  • High-throughput measurements of single cell rheology by atomic force microscopy               
    K. Kuribayashi-Shigetomi, R. Takahashi, A. Subagyo, K. Sueoka, T. Okajima
    Hyper Bio Assembler for 3D Cellular Systems, Chapter 4 (57-67), Springer, 2015, 2015, [Joint work]
  • 原子間力顕微鏡による超高速細胞メカニクス計測技術               
    高橋亮輔, 岡嶋孝治
    ケミカルエンジニヤリング(先端計測技術開発の展望:9月号)(2015), 2015
  • 細胞の物理学:SPMによる定量計測               
    Okajima, Takaharu
    表面科学 35, 544-544(2014), 2014, [Joint work]
  • 走査プローブ顕微鏡による細胞物性測定:原子間力顕微鏡とイオンコンダクタンス顕微鏡(総説)               
    岡嶋孝治
    化学工業 (2013), 2013
  • 原子間力顕微鏡による細胞力学の定量計測(解説)               
    岡嶋 孝治
    検査技術 (2013), 2013
  • 原子間力顕微鏡による細胞レオロジー測定(総説)               
    岡嶋孝治
    日本膜学会「膜」38, 76-81 (2013), 2013
  • 1細胞レオロジー測定 : 原子間力顕微鏡による細胞レオロジー計測の新手法               
    岡嶋 孝治, 水谷 祐輔
    生物物理 52, 5, 230-233, Sep. 2012
  • 細胞力学計測のためのマイクロ加工基板を用いた原子間力顕微鏡法               
    岡嶋 孝治, 水谷 祐輔
    表面科学33(8) 461-466, Aug. 2012
  • 1細胞レオロジー測定:原子間力顕微鏡による細胞レオロジー計測の新手法(総説)               
    岡嶋孝治, 水谷祐輔
    日本生物物理学会誌「生物物理」52, 230-233 (2012), 2012
  • 走査プローブ顕微鏡(実験物理科学シリーズ6)               
    共立出版, 2009
  • 原子間力顕微鏡による生細胞ナノレオロジー計測               
    岡嶋孝治, 徳本洋志
    日本レオロジー学会誌 vol.36, No.2, p. 81-86, Apr. 2008, [Contributor]
  • ナノテクのための物理入門               
    共立出版, 2007
  • BioNics[バイオニクス]               
    オーム社, 2005
  • ソフトナノテクノロジー —バイオマテリアル革命—               
    シーエムシー出版, 2005

Lectures, oral presentations, etc.

  • Mapping mechanical properties of single cells during embryogenesis using atomic force microscopy               
    T. Okajima
    AFM BioMED conference, (2022.8.29-9.2, Okazaki), Dec. 2022, English, Invited oral presentation
    [Invited]
  • 多細胞メカニクスの時空間マッピング測定               
    岡嶋 孝治
    ナノプローブテクノロジー第167委員会 第103回研究会、高分子×SPM(2022.7.27、大岡山), Jul. 2022, Japanese, Invited oral presentation
    [Invited]
  • 原子間力顕微鏡による発生胚の力学動態の計測               
    岡嶋 孝治
    第61回日本生体医工学会、細胞アッセイ:細胞動態の把握と計測、(2022.6.28-30、新潟), Jun. 2022, Japanese, Invited oral presentation
    [Invited]
  • 発生胚表面のメカニクス:原子間力顕微鏡測定               
    岡嶋孝治
    日本膜学会の第44回年会、(2022.6.9-10、早稲田), Jun. 2022, Japanese, Invited oral presentation
    [Invited]
  • Nanomechanics of single cells and tissues revealed by atomic force microscopy               
    T. Okajima
    Pacifichem 2021, Experimental and Computational Analysis of the Nano-Bio Interface for Sustainable Nanotechnology, (Dec.16-21, 2021, Online), Dec. 2021, English, Invited oral presentation
  • Measuring mechanical properties of developing embryo with AFM               
    T. Okajima
    MRS Fall meeting 2021, Frontiers in Scanning Probe Microscopy—Beyond Imaging of Soft Materials, (2021.12.6-8, Boston/Online Hybrid), Dec. 2021, English, Invited oral presentation
    [Invited]
  • 発生胚のAFM計測:現状と課題               
    岡嶋孝治
    第17回バイオオプティクス研究会(2021年12月10-11日、山口/オンライン), Dec. 2021, Japanese, Invited oral presentation
    [Invited]
  • AFMによる受精卵の発生過程の力学計測               
    岡嶋孝治
    日本顕微鏡学会 第77回学術講演会、先端ナノプローブ法による高分解能局所物性・オペランド計測(2021年6月14日、つくば), Jun. 2021, Japanese, Invited oral presentation
    [Invited]
  • AFMによる発生胚のメカニクス               
    岡嶋孝治
    2021年応物春季学術講演会, 革新的走査型プローブ顕微鏡技術で拓くナノプローブ生命科学の新展開(2021.3.16-19、onine), Mar. 2021, Japanese, Invited oral presentation
    [Invited]
  • 原子間力顕微鏡:細胞・組織のメカニクス測定               
    岡嶋孝治
    日本薬剤学会・物性FGセミナー2020・顕微鏡法で医薬品原薬・製剤を“顕わ”にする―低分子から細胞まで―、2021.2.19、オンライン, Feb. 2021, Japanese, Invited oral presentation
    [Invited]
  • Nanomechanics of cell and tissue probed by atomic force microscopy               
    T. Okajima
    6th International Conference on Electronic Materials and Nanotechnology for Green Environment (ENGE 2020) (2020. 11.1-4, Korea_online), Nov. 2020, English, Invited oral presentation
    [Invited]
  • AFMによる細胞・組織の力学情報の定量化               
    岡嶋孝治
    2020年第81回応用物理学会秋季学術講演会・多次元計測技術とデータサイエンスの融合によるバイオイメージング・センシング技術の進展(京都オンライン、2020.9.9), Sep. 2020, Japanese, Invited oral presentation
    [Invited]
  • バイオAFM計測:1細胞から多細胞へ               
    岡嶋孝治
    第59回日本生体医工学会大会(岡山オンライン、2020.5.25-27), May 2020, Japanese, Invited oral presentation
    [Invited]
  • 原子間力顕微鏡による細胞・組織メカニクスの定量化               
    岡嶋孝治
    第67回応用物理学会春期学術講演会・多次元計測技術とデータサイエンスの融合によるバイオイメージング・センシングの将来、2020年3月12日-15日、東京, Mar. 2020, Japanese, Invited oral presentation
    [Invited]
  • AFMによる1細胞・多細胞系のナノ力学計測               
    岡嶋孝治
    2019年日本表面真空学会学術講演会・局所分光とバイオ計測(プローブ顕微鏡部会)(つくば、2019.10.28-30), Oct. 2019, Japanese, Invited oral presentation
    [Invited]
  • AFMによる多細胞系のメカニクス               
    岡嶋孝治
    日本顕微鏡学会 ソフトマテリアル研究部会 第6回講演会(宮城蔵王、2019.10.25-26), Oct. 2019
    [Invited]
  • AFMよる1細胞・細胞集団のナノメカニクス               
    岡嶋孝治
    日本顕微鏡学会第75回学術講演会・原子間力顕微鏡技術のパラダイムシフト~原子から細胞まで階層をまたぐ構造・機能解析~(名古屋、2019.6.17-19), Jun. 2019, Japanese, Invited oral presentation
    [Invited]

Affiliated academic society

  • American Society for Cell Biology               
  • 表面科学会               
  • 日本生物物理学会               
  • 応用物理学会               
  • Biophysical Society(米国)               

Research Themes

  • 階層性自己組織化複合材料デザイン(代表者:松本卓也)               
    CREST
    Oct. 2022 - Mar. 2028
    岡嶋孝治
    JST, 分解・劣化・安定化の精密材料科学, Coinvestigator, JPMJCR22L5
  • プローブ顕微鏡による発生胚の遺伝子・力学的相互作用の解明
    科学研究費助成事業
    01 Apr. 2024 - 31 Mar. 2027
    岡嶋 孝治, 道上 達男, 繁富 香織
    日本学術振興会, 基盤研究(A), 北海道大学, 24H00412
  • 原子間力顕微鏡による1細胞診断技術の高速化               
    基盤研究(B)
    Apr. 2021 - Mar. 2024
    岡嶋 孝治
    Principal investigator
  • The establishment of tissue elasticity modalities and aging control
    Grants-in-Aid for Scientific Research
    09 Jul. 2021 - 31 Mar. 2023
    早野 元詞, 岡嶋 孝治
    Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Keio University, 21K18608
  • 原子間力顕微鏡を用いた発生胚メカニクスの包括的マッピング計測技術の開発               
    挑戦的研究(萌芽)
    Apr. 2021 - Mar. 2023
    岡嶋 孝治
    Principal investigator
  • In vitro pharmacological assessment of pulmonary arterial hypertension by atomic force microscopy
    Grants-in-Aid for Scientific Research
    01 Apr. 2019 - 31 Mar. 2022
    Kogaki Shigetoyo
    We established primary culture lines of pulmonary arterial smooth muscle cells (PASMCs) from a patient of idiopathic pulmonary arterial hypertension (IPAH) and a healthy control PASMC from donor lung. We analyzed their cellular mechanical properties by using atomic force microscopy. We administered the clinical available pulmonary vasodilators, then analyzed the cellular rheology of PASMCs. Sildenafil, a phosphodiesterase 5 inhibitor, could reduce cellular elasticity of PAH-PASMCs. Macitentan and riociguat could also reduce the cellular elasticity and could increase cellular fluidity of PAH-PASMCs. The combination therapy of macitentan and riociguat could effectively reduce cellular elasticity in lower concentration of the drugs, suggesting that the usefulness of combination therapy in PAH treatment.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Osaka University, 19K08276
  • マルチプローブ顕微鏡技術を用いた発生胚メカニクス計測法の開発               
    挑戦的研究(萌芽)
    Jul. 2019 - Mar. 2021
    岡嶋 孝治
    Principal investigator, Competitive research funding
  • 細胞のエルゴード性を利用したがん細胞力学診断・アッセイ法の開発               
    基盤研究(B)
    Apr. 2018 - Mar. 2021
    岡嶋 孝治
    Principal investigator, Competitive research funding
  • Rheological analysis of pulmonary smooth muscle cells in pulmonary atrterial hypertension by using atomic force microscope
    Grants-in-Aid for Scientific Research
    01 Apr. 2016 - 31 Mar. 2019
    Narita Jun, KATSURAGI SHINICHI
    Recently, it was reported that the compliance of proximal pulmonary artery wall played important roles in the pathogenesis of pulmonary arterial hypertension (PAH) as well as distal pulmmonary vascular resistance did. However it was still unknown how the elasticity of pulmonary arterial smooth muscle cell (PASMC) itself can be affected in the patients of PAH. In this study, we revealed that there was a high elasticity population in PASMC of PAH patient. Additionally, Sldenafil, a drug for PAH could reduce the population of high elasticity in PASMC derived from PAH patients.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Osaka University, 16K10065
  • 原子間力顕微鏡を用いた発生胚メカニクスの網羅解析法の開発               
    挑戦的研究(萌芽)
    Apr. 2017 - Mar. 2019
    Okajima, Takaharu
    Principal investigator, Competitive research funding
  • ヒト間葉系幹細胞治療技術の開発とその最適化に関する研究               
    二国間交流事業 日本—シンガポール 共同研究
    Apr. 2015 - Mar. 2017
    Okajima, Takaharu
    Principal investigator, Competitive research funding
  • 多重周波数フォースモジュレーション顕微鏡の開発:細胞レオロジー変数のイメージング               
    挑戦的萌芽研究
    Apr. 2015 - Mar. 2017
    Okajima, Takaharu
    Principal investigator, Competitive research funding
  • 原子間力顕微鏡による生体組織力学物性のその場分離計測技術の開発               
    新学術領域研究(研究領域提案型)バイオアセンブラ
    Apr. 2014 - Mar. 2016
    Okajima, Takaharu
    Principal investigator, Competitive research funding
  • プローブ顕微鏡による細胞間力学的相互作用の時空間揺らぎの研究               
    新学術領域研究(研究領域提案型) ゆらぎと構造
    Apr. 2014 - Mar. 2016
    Okajima, Takaharu
    Principal investigator, Competitive research funding
  • イオンコンダクタンス顕微鏡を用いた細胞膜揺らぎの定量イメージング技術の開発               
    挑戦的萌芽研究
    Apr. 2013 - Mar. 2015
    岡嶋 孝治
    Principal investigator, Competitive research funding
  • 原子間力顕微鏡を用いた細胞力学伝播関数の時空間定量解析               
    基盤研究(B)
    2013 - 2015
    岡嶋 孝治
    Principal investigator, Competitive research funding
  • 原子間力顕微鏡による超高速細胞レオロジー分離技術の開発               
    新学術領域研究(研究領域提案型)バイオアセンブラ
    Apr. 2012 - Mar. 2014
    岡嶋孝治
    Principal investigator, Competitive research funding
  • 原子間力顕微鏡を用いたがん細胞力学診断技術の開発               
    Apr. 2011 - Mar. 2013
    岡嶋 孝治
    挑戦的萌芽研究, Principal investigator, Competitive research funding
  • 原子間力顕微鏡法を用いたがん細胞診断自動解析法の開発               
    研究成果最適展開支援プログラム(A-STEP)
    2012 - 2013
    岡嶋 孝治
    Principal investigator, Competitive research funding
  • 原子間力顕微鏡を用いた多数細胞力学の多変量解析の研究               
    Apr. 2009 - Mar. 2012
    岡嶋 孝治
    基盤研究(B), Principal investigator, Competitive research funding
  • 細胞周期制御下の細胞界面ナノダイナミクスの研究               
    Apr. 2009 - Mar. 2011
    岡嶋 孝治
    特定領域研究・高次系分子科学, Principal investigator, Competitive research funding
  • 走査プローブ顕微鏡と蛍光相関分光法による生細胞界面の1分子ダイナミクスの研究               
    Apr. 2008 - Mar. 2010
    岡嶋 孝治
    特定領域研究・高次系分子科学, Principal investigator, Competitive research funding
  • 単一細胞表層の全方向ナノダイナミクス計測技術の開発               
    産業技術研究助成事業
    2006 - 2009
    岡嶋孝治
    NEDO, 産業技術研究助成事業, Principal investigator
  • 糖鎖1分子のナノ力学刺激に対する応答ダイナミクスの研究               
    Apr. 2005 - Mar. 2007
    岡嶋 孝治
    萌芽研究, Principal investigator, Competitive research funding
  • Research of Carbon-Nanotube-AFM for Exploring Inside Biological Cells
    Grants-in-Aid for Scientific Research
    2004 - 2006
    TOKUMOTO Hiroshi, OKAJIMA Takaharu, AZEHARA Hiroaki
    AFM is the very powerful tool for surface science and is now extending its application to the biotechnology field where a single molecule and protein will be observed in an appropriate condition. And now in this project, we will try to develop a technique to explore the internal structures or functions of the cell. Here, we will develop key techniques for bio-AFM using carbon nanotube(CNT) as its tip: First we have prepared stable CNT-AFM tips fixed onto commercial Si-AFM tips. At the same time, we fabricated the artificial lipid bilayer membranes using dimyristoyl phosphatidyl choline (DMPC) molecules. And then we penetrated the former CNT-AFM tip through the DMPC bilayer film while measuring the forces between the tip and the membrane. Further, we successfully modified chemically an end of the CNT tip, where -COOH's were fixed, by simply cutting CNT in a low vacuum environment of SEM. As a test sample, we prepared patterned structure on Au substrate consisting of hydrophobic and hydrophilic parts with the aid of the micro-contact printing technique. By measuring the weak forces between chemically modified CNT-tip and the test sample, we have discriminated the two parts very clearly, indicating that we have successfully demonstrated the ability of the chemical force AFM. Under this success in mind, we will be able to recognize the individual bases in a DNA molecule using this technique. Inside and outside of cells, there is various bio-molecules which may interact nonspecifically with AFM tips. We measured these forces using PEG polymer incorporated into polymer gels and found specific forces as expected. At the same time, we have developed the good technique to manipulate a single polymer. By measuring the stress relaxation of HepG2 cells using AFM, we have obtained information on dynamic elasticity of the living cells. From these results, we will open a new mechanical technique for obtaining the information to understand the relationship between structure and function of living cells.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 16310078
  • 生体分子のマイクロ秒粘弾性測定               
    Apr. 2003 - Mar. 2005
    岡嶋 孝治
    若手研究(B), Principal investigator, Competitive research funding
  • 原子間力顕微鏡を用いた単一高分子鎖の相転移における力学緩和の測定               
    若手研究(B)
    Apr. 1999 - Mar. 2001
    岡嶋 孝治
    Principal investigator, Competitive research funding
  • シェアフォース顕微鏡におけるプローブ・表面間相互作用の起源の解明               
    奨励研究(A)
    Apr. 1997 - Mar. 1999
    岡嶋 孝治
    Principal investigator, Competitive research funding
  • Research on Basic Properties and Structure of ionized Gels
    Grants-in-Aid for Scientific Research
    1998 - 1999
    HIROTSU Shunsuke, OKAJIMA Takaharu
    Reasearch on the mechanism of the volume phase transition : Traditional view on the mechanism of the volume phase transition in gels has been critically analyzed. We have proposed a new model of the volume phase transition in which the shrunken phase is not a get but a precipitated network. Experimental evidences supporting this new model have been obtained by dynamic light scattering and elastic relaxation measurements.
    Meso-phase formation in ionized gels : Formation of mesoscopic structure accompanied by charge separation has been investigated by static and dynamic light scattering. A clear evidence of meso-phase formation have been obtained both in relaxation and intensity of the scattered light. This is the first example demonstrating that the meso-phase can be studied not only by neutron but also visible light.
    Elastic measurements near the volume phase transition : A new apparatus for mesuring elastic constants and elastic relaxation has been built. Using this apparatus elastic behaviors around the volume phase transition have been succesfully measured.
    Structural differences between biologal polymer gels and synthesized gels : In terms of light scattering measurements, the structural difference between acrylamide family gels and bilogical polysaccharides has been studied. The difference is considered to be due mainly to the difference in crosslinking mechanisms.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), TOKYO INSTITUTE OF TECHNOLOGY, 10440116
  • 温度敏感性ゲルを用いた細胞培養による細胞の応力応答の研究
    科学研究費助成事業
    1997 - 1998
    弘津 俊輔, 岡嶋 孝治
    本年度に行った研究とその成果を以下にまとめた。
    1) 温度敏感性ゲルの相転移を利用した、細胞への応力印加の実験。: これについては、ゼラチンを混入したNIPAゲルを用いて、試行実験を行った。結果は、膨潤相から収縮相への変化に関しては目立った効果は見られなかった。現在、細胞に有効に力を印可する方法を開発すると共に、収縮相から膨潤相への転移の効果も調べつつある。
    2) 化学架橋ゼラチンゲル基盤の合成: 細胞接着性が良好で、しかも比較的自由に弾性的性質を調節出来る基盤として、ゼラチンとコハク化ゼラチンの混合物をカルボジイミドで架橋したゲルを開発した。ゼラチンとコハク化ゼラチンの混合比を変えることにより、弾性率が同じで、膨潤率が異なるゲルを得ることが出来た。
    3) ゲルの弾性率測定: 電子天秤を利用してゲルに一定の加重を印可し、その変形から、ゲルの静的弾性率(ヤング率、合成率、および体積弾性率)を決定した。
    4) ゲル基盤の弾性的性質と培養細胞の形状との関係
    基盤(足場)と細胞との力学的相互作用が、細胞の機能、増殖能、形状などに与える影響を理解するためには、基盤の弾性的性質が細胞に与える効果を調べる必要がある。上記の方針で、弾性率を測定したゲルで、マウス3T3繊維芽細胞を培養してその形状を調べた結果、細胞形状は基盤の堅さに強く依存することが分かった。具体的には、基盤ゲルのヤング率が1x10^4[dyn/cm^2]以上の場合、7x10^3〜1x10^4[dyn/cm^2]、7x10^3[dyn/cm^2]以下の場合のそれぞれに応じて、細胞の外径および増殖のパターンに顕著な相違が見られた。より詳しい解析を実行中である。
    日本学術振興会, 萌芽的研究, 東京工業大学, 09875243

Industrial Property Rights

  • 原子間力顕微鏡による単一細胞の力学的診断方法と診断装置               
    Patent right, 岡嶋孝治, 高橋亮輔
    特願2017-55414, 22 Mar. 2017
  • 酵母細胞の力学特性の測定方法、及び力学特性に基づいた酵母細胞のスクリーニング方法               
    Patent right, 有友亮太, 潮井徹, 岡嶋孝治, 田中良昌
    特願特願2016-176540, 09 Sep. 2016
  • 細胞足場材料、細胞モデル、及び細胞足場材料の製造方法               
    Patent right, 田中あや, 手島哲彦, 中島寛, 岡嶋孝治, 藤井裕紀
    特願2017-032733, 23 Feb. 2016
  • 細胞の複素弾性率の計測方法および計測システム               
    Patent right, 岡嶋孝治, 高橋亮輔
    特願2014-136721, 02 Jul. 2014
  • 単一細胞の力学特性の計測方法および計測装置               
    Patent right, 岡嶋孝治, 蔡萍根, 水谷祐輔, 土屋雅博
    特願2011-184403, 26 Aug. 2011
    特願2011-184403
  • 原子間力顕微鏡用のコロイドプローブカンチレバーの製造方法および製造装置               
    Patent right
    特願2007-287126
  • Surface Position Measuring Method and Surface Position Measuring Device               
    Patent right
    WO 2006/073068
  • Device and Method for Measuring Molecule Using Gel Substrate Material               
    Patent right
    WO 2006/028135
  • Molecular Measuring Device and Molecule Measuring Method               
    Patent right
    WO 2006/011348