2022, 北海道大学, excellent teacher　　　　　　　　　　　　　　　

2021, 北海道大学, excellent teacher　　　　　　　　　　　　　　　

2020, 北海道大学, excellent teacher　　　　　　　　　　　　　　　

2018, 北海道大学, excellent teacher　　　　　　　　　　　　　　　
伊 敏

Histochemical assessment on osteoclasts in long bones of toll-like receptor 2 (TLR2) deficient mice.
Takafumi Muneyama, Tomoka Hasegawa, Yimin, Tomomaya Yamamoto, Hiromi Hongo, Mai Haraguchi-Kitakamae, Miki Abe, Haruhi Maruoka, Hotaka Ishizu, Tomohiro Shimizu, Yasuyuki Sasano, Minqi Li, Norio Amizuka
Journal of oral biosciences, 21 Apr. 2023, [International Magazine]
English, Scientific journal, OBJECTIVE: Toll-like receptor 2 (TLR2), recognizes a wide variety of pathogen-associated molecular patterns such as lipopolysaccharides, peptidoglycans, and lipopeptides, and is generally believed to be present in monocytes, macrophages, dendritic cells, and vascular endothelial cells. However, no histological examination of osteoclasts, which differentiate from precursors common to macrophages/monocytes, has been performed in a non-infected state of TLR2 deficiency. The objective of this study was to examine the histological properties and function of osteoclasts in the long bones of 8-week-old male TLR2 deficient (TLR2-/-) mice to gain insight into TLR2 function in biological circumstances without microbial infection. METHODS: Eight-week-old male wild-type and TLR2-/- mice were fixed with paraformaldehyde solution, and their tibiae and femora were used for micro-CT analysis, immunohistochemistry, transmission electron microscopy, and real-time PCR analysis. RESULTS: TLR2-/- tibiae and femora exhibited increased bone volume of metaphyseal trabeculae and elevated numbers of TRAP-positive osteoclasts. However, the number of multinucleated TRAP-positive osteoclasts was reduced, whereas mononuclear TRAP-positive cells increased, despite the high expression levels of Dc-Stamp and Oc-Stamp. Although TRAP-positive multinucleated and mononuclear osteoclasts showed the immunoreactivity and elevated expression of RANK and siglec-15, they revealed weak cathepsin K-positivity and less incorporation of the mineralized bone matrix, and often missing ruffled borders. It seemed likely that, despite the increased numbers, TLR2-/- osteoclasts reduced cell fusion and bone resorption activity. CONCLUSION: It seems likely that even without bacterial infection, TLR2 might participate in cell fusion and subsequent bone resorption of osteoclasts.

Histochemical assessment of accelerated bone remodeling and reduced mineralization in Il-6 deficient mice.
Yasuhito Moritani, Tomoka Hasegawa, Tomomaya Yamamoto, Hiromi Hongo, Yimin, Miki Abe, Hirona Yoshino, Ko Nakanishi, Haruhi Maruoka, Hotaka Ishizu, Tomohiro Shimizu, Masahiko Takahata, Norimasa Iwasaki, Minqi Li, Kanchu Tei, Yoichi Ohiro, Norio Amizuka
Journal of oral biosciences, 11 Oct. 2022, [International Magazine]
English, Scientific journal, OBJECTIVES: Interleukin-6 (IL-6) contributes to the regulation of functions in various tissues and organs. Even though IL-6 has been reported to modulate bone metabolism in previous studies, this finding is controversial. This study aims to evaluate the possible involvement of IL-6 in bone metabolism by examining the histological activity of osteoblasts and osteoclasts in the femora of Il-6 deficient (Il-6-/-) mice. METHODS: Eight-week-old male Il-6-/- mice and their wild-type littermates were fixed with a paraformaldehyde solution, and their femora were extracted for micro-CT analysis, immunohistochemistry, and real-time PCR analysis. RESULTS: Il-6-/- femora showed an increased bone volume/tissue volume (TV) but a reduced bone mineral density compared with the wild-type. Furthermore, the tissue-nonspecific alkaline phosphatase positive area/TV ratio, the expression of Runx2, Osterix, and Rankl, and the number of tartrate-resistant acid phosphatase-positive osteoclasts were all increased in the Il-6-/- mice. A considerable number of unmineralized areas within the bone matrix and abundant sclerostin-reactive osteocytes were observed in Il-6-/- femoral metaphyses but not in the wild-type. Interestingly, the gene expression of Cd206 was elevated in Il-6-/- femora, and many F4/80-positive macrophages/monocytes and CD206-immunoreactive macrophages in the primary trabeculae had migrated closer to the growth plate, where intense RANKL immunoreactivity was detected. These results suggest that, in an IL-6-deficient state, CD206-positive macrophages may differentiate into osteoclasts when in contact with RANKL-reactive osteoblastic cells. CONCLUSION: In a state of IL-6 deficiency, the population and cell activities of osteoblast, osteoclasts, and macrophages seemed to be facilitated, except for the reduced mineralization in bone.

Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells.
Ayako Yokoyama, Tomoka Hasegawa, Toru Hiraga, Tamaki Yamada, Yimin, Hiromi Hongo, Tomomaya Yamamoto, Miki Abe, Taiji Yoshida, Yasuo Imanishi, Shinichiro Kuroshima, Muneteru Sasaki, Paulo Henrique Luiz de Fraitas, Minqi Li, Norio Amizuka, Yutaka Yamazaki
Journal of bone and mineral metabolism, 39, 5, 810, 823, Sep. 2021, [Domestic magazines]
English, Scientific journal, INTRODUCTION: After the onset of bone metastasis, tumor cells appear to modify surrounding microenvironments for their benefit, and particularly, the levels of circulating fibroblast growth factor (FGF) 23 in patients with tumors have been highlighted. MATERIALS AND METHODS: We have attempted to verify if human breast carcinoma MDA-MB-231 cells metastasized in the long bone of nu/nu mice would synthesize FGF23. Serum concentrations of calcium, phosphate (Pi) and FGF23 were measured in control nu/nu mice, bone-metastasized mice, and mice with mammary gland injected with MDA-MB-231 cells mimicking primary mammary tumors. RESULTS AND CONCLUSIONS: MDA-MB-231 cells revealed intense FGF23 reactivity in metastasized lesions, whereas MDA-MB-231 cells cultured in vitro or when injected into the mammary glands (without bone metastasis) showed weak FGF23 immunoreactivity. Although the bone-metastasized MDA-MB-231 cells abundantly synthesized FGF23, osteocytes adjacent to the FGF23-immunopositive tumors, unlike intact osteocytes, showed no FGF23. Despite significantly elevated serum FGF23 levels in bone-metastasized mice, there was no significant decrease in the serum Pi concentration when compared with the intact mice and mice with a mass of MDA-MB-231 cells in mammary glands. The metastasized femora showed increased expression and FGFR1 immunoreactivity in fibroblastic stromal cells, whereas femora of control mice showed no obvious FGFR1 immunoreactivity. Taken together, it seems likely that MDA-MB-231 cells synthesize FGF23 when metastasized to a bone, and thus affect FGFR1-positive stromal cells in the metastasized tumor nest in a paracrine manner.

Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone.
Tomoya Nagai, Tomoka Hasegawa, Yimin, Tomomaya Yamamoto, Hiromi Hongo, Miki Abe, Taiji Yoshida, Ayako Yokoyama, Paulo Henrique Luiz de Freitas, Minqi Li, Atsuro Yokoyama, Norio Amizuka
Histochemistry and cell biology, 155, 3, 369, 380, Mar. 2021, [International Magazine]
English, Scientific journal, In this study, we examined the immunolocalization of podoplanin/E11, CD44, actin filaments, and phosphorylated ezrin in the osteoblasts on the verge of differentiating into osteocytes in murine femora and tibiae. When observing under stimulated emission depletion microscopy, unlike podoplanin-negative osteoblasts, podoplanin-positive osteoblasts showed a rearranged assembly of actin filaments along the cell membranes which resembled that of embedded osteocytes. In the metaphysis, i.e., the bone remodeling site, CD44-bearing osteoclasts were either proximal to or in contact with podoplanin-positive osteoblasts, but the podoplanin-positive osteoblasts also localized CD44 on their own cell surface. These podoplanin-positive osteoblasts, which either possessed CD44 on their cell surface or were close to CD44-bearing osteoclasts, showed phosphorylated ezrin-positivity on the cell membranes. Therefore, the CD44/podoplanin interaction on the cell surface may be involved in the osteoblastic differentiation into osteocytes in the metaphyses, via the mediation of podoplanin-driven ezrin phosphorylation and the subsequent reorganized assembly of actin filaments. Consistently, the protein expression of phosphorylated ezrin was increased after CD44 administration in calvarial culture. Conversely, in modeling sites such as the cortical bones, podoplanin-positive osteoblasts were uniformly localized at certain intervals even without contact with CD44-positive bone marrow cells; furthermore, they also exhibited phosphorylated ezrin immunoreactivity along their cell membranes. Taken together, it seems likely that the CD44/podoplanin interaction is involved in osteoblastic differentiation into osteocytes in the bone remodeling area but not in modeling sites.

Histological observation on the initial stage of vascular invasion into the secondary ossification of murine femoral epiphyseal cartilage
Keiji Hashimoto, Tomoka Hasegawa, Tomomaya Yamamoto, Hiromi Hongo, Yimin, Miki Abe, Alireza Nasoori, Ko Nakanishi, Haruhi Maruoka, Yasuhito Morimoto, Keisuke Kubota, Tomohiro Shimizu, Mai Haraguchi, Masahiko Takahata, Norimasa Iwasaki, Minqi Li, Toshiaki Fujisawa, Norio Amizuka
Biomedical Research (Japan), 42, 4, 139, 151, 2021
Scientific journal

Histological observation on the initial stage of vascular invasion into the secondary ossification of murine femoral epiphyseal cartilage
Keiji Hashimoto, Tomoka Hasegawa, Tomomaya Yamamoto, Hiromi Hongo, Yimin, Miki Abe, Alireza Nasoori, Ko Nakanishi, Haruhi Maruoka, Yasuhito Morimoto, Keisuke Kubota, Tomohiro Shimizu, Mai Haraguchi, Masahiko Takahata, Norimasa Iwasaki, Minqi Li, Toshiaki Fujisawa, Norio Amizuka
BIOMEDICAL RESEARCH-TOKYO, 42, 4, 139, 151, 2021
English, Scientific journal

Extracts of bilberry (Vaccinium myrtillus L.) fruits improve liver steatosis and injury in mice by preventing lipid accumulation and cell death.
Haga S, YiMin, Yamaki H, Jin S, Sogon T, Morita N, Ozaki M
Bioscience, biotechnology, and biochemistry, 83, 11, 2110, 2120, Oxford University Press (OUP), Nov. 2019, [Peer-reviewed]
Scientific journal,
Bilberry has been reported to have anti-oxidant and anti-inflammatory properties. We studied the effect of bilberry (Vaccinium myrtillus L.) fruits extracts (BEs) on the pathogenesis caused by lipid accumulation in fatty liver and non-alcoholic steatohepatitis (NASH). 5 μg/ml of BEs was enough to suppress lipid accumulation in the fatty liver model of the mouse hepatic AML12 cells. BEs increased cell viability and anti-oxidant capacity, presumably by activating (phosphorylating) Akt/STAT3 and inducing MnSOD/catalase. BEs also significantly reduced Rubicon and induced p62/SQSTM1, possibly contributing to reduce cellular lipids (lipophagy). When the mice were fed supplemented with BEs (5% or 10%, w/w), hepatic steatosis, injury, and hypercholesterolemia/hyperglycemia were significantly improved. Furthermore, histological and cytokine studies indicated that BEs possibly suppress hepatic inflammation (hepatitis) and fibrosis. Therefore, BEs improved liver steatosis and injury, and potentially suppress fibrosis by suppressing inflammatory response, which therefore may prevent the progression of fatty liver to NASH.

Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats
Tomoka Hasegawa, Yukina Miyamoto-Takasaki, Miki Abe, Zixuan Qiu, Tomomaya Yamamoto, Yimin, Taiji Yoshida, Hirona Yoshino, Hiromi Hongo, Ayako Yokoyama, Muneteru Sasaki, Shinichiro Kuroshima, Kuniko Hara, Masatoshi Kobayashi, Yasuhiro Akiyama, Takeyasu Maeda, Paulo Henrique Luiz de Freitas, Minqi Li, Norio Amizuka
MICROSCOPY, 68, 5, 349, 358, Oct. 2019
English, Scientific journal

Immunolocalization of podoplanin/E11/gp38, CD44, and endomucin in the odontoblastic cell layer of murine tooth germs.
Naznin Khadiza, Tomoka Hasegawa, Tomoya Nagai, Tomomaya Yamamoto, Yukina Miyamoto-Takasaki, Hiromi Hongo, Miki Abe, Mai Haraguchi, Tsuneyuki Yamamoto, Yimin, Zixuan Qiu, Muneteru Sasaki, Shinichiro Kuroshima, Hayato Ohshima, Paulo Henrique Luiz de Freitas, Minqi Li, Yasutaka Yawaka, Norio Amizuka
Biomedical research (Tokyo, Japan), 40, 4, 133, 143, 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, In this study, we attempted to localize the immunoreactivities of podoplanin/E11/gp38 and CD44, a counterpart possessing a high affinity to podoplanin/E11/gp38, as well as endomucin-immunoreactive blood vessels in the regions of odontoblast layers and the underlying sub-odontoblastic layers in murine tooth germs. Endomucin-reactive small blood vessels were scattered throughout the dental papillae of the tooth germs at postnatal day 1 but came to be localized close to the odontoblast/sub-odontoblastic layers until day 3. After postnatal day 5, small blood vessels were seen in odontoblast cell layers, while blood vessels with relatively larger diameters were seen forming in sub-odontoblastic layers. Immunoreactivities of podoplanin/E11/gp38 and CD44 were not detectable in the cells of dental papillae facing the inner enamel epithelium at postnatal day 1. However, at around postnatal days 3-5, podoplanin/E11/gp38 was localized in the odontoblast layer but not in the sub-odontoblastic layer, whereas CD44 was observed in the sub-odontoblastic layer but not in the odontoblast layer. The exclusive immunolocalization of podoplanin/E11/gp38 and CD44 in the odontoblast layers and sub-odontoblastic layers was seen after postnatal day 3 of the tooth germs, when the mesenchymal cells of dental papillae have already differentiated into mature odontoblasts at the cusp tip. Taken together, it seems likely that endomucin-reactive small blood vessels extended to the podoplanin/E11/gp38-positive odontoblast layers, whereas endomucin-reactive large blood vessels were already present in CD44-immmunopositive sub-odontoblastic layer, indicating the cellular regulation on the vascularization of endomucin-reactive endothelial cells during odontogenesis of the tooth germs.

Photo-activatable akt probe: A new tool to study the akt-dependent physiopathology of cancer cells
Sanae Haga, Takeaki Ozawa, Naoki Morita, Mami Asano, Shigeki Jin, Yimin, Michitaka Ozaki
Oncology Research, 26, 3, 467, 472, Cognizant Communication Corporation, 2018, [Peer-reviewed]
English, Scientific journal

Relevance of FXR-p62/SQSTM1 pathway for survival and protection of mouse hepatocytes and liver, especially with steatosis
Sanae Haga, Yimin, Michitaka Ozaki
BMC GASTROENTEROLOGY, 17, Jan. 2017, [Peer-reviewed]
English, Scientific journal

Immunolocalization of MMP9 and MMP2 in osteolytic metastasis originating from MDA-MB-231 human breast cancer cells
Bo Liu, Jian Cui, Jing Sun, Juan Li, Xiuchun Han, Jie Guo, Min Yi, Norio Amizuka, Xin Xu, Minqi Li
MOLECULAR MEDICINE REPORTS, 14, 2, 1099, 1106, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Oyster extracts attenuate pathological changes in non-alcoholic steatohepatitis (NASH) mouse model
Mitsugu Watanabe, Hirotoshi Fuda, Hiroaki Okabe, Sae Joko, Yusuke Miura, Shu-Ping Hui, Yimin, Naohiro Hamaoka, Emiko Miki, Hitoshi Chiba
JOURNAL OF FUNCTIONAL FOODS, 20, 516, 531, Jan. 2016, [Peer-reviewed]
English, Scientific journal

Long-Term Administration of High-Fat Diet Corrects Abnormal Bone Remodeling in the Tibiae of Interleukin-6-Deficient Mice
Wei Feng, Bo Liu, Di Liu, Tomoka Hasegawa, Wei Wang, Xiuchun Han, Jian Cui, Yimin, Kimimitsu Oda, Norio Amizuka, Minqi Li
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 64, 1, 42, 53, Jan. 2016, [Peer-reviewed]
English, Scientific journal

Altered distribution of HMGB1 in the periodontal ligament of periostin-deficient mice subjected to Waldo's orthodontic tooth movement
Juan Li, Wei Feng, Bo Liu, Bao Sun, Xiuchun Han, Juan Du, Jing Sun, Yimin, Jian Cui, Jie Guo, Akira Kudo, Norio Amizuka, Minqi Li
JOURNAL OF MOLECULAR HISTOLOGY, 46, 3, 303, 311, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Histochemical evidence of zoledronate inhibiting c-src expression and interfering with CD44/OPN-mediated osteoclast adhesion in the tibiae of mice
Hongrui Liu, Jian Cui, Jing Sun, Juan Du, Wei Feng, Bao Sun, Juan Li, Xiuchun Han, Bo Liu, Yimin, Kimimitsu Oda, Norio Amizuka, Minqi Li
JOURNAL OF MOLECULAR HISTOLOGY, 46, 3, 313, 323, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Local administration of calcitriol positively influences bone remodeling and maturation during restoration of mandibular bone defects in rats
Hongrui Liu, Jian Cui, Wei Feng, Shengyu Lv, Juan Du, Jing Sun, Xiuchun Han, Zhenming Wang, Xiong Lu, Yimin, Kimimitsu Oda, Norio Amizuka, Minqi Li
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 49, 14, 24, Apr. 2015, [Peer-reviewed]
English, Scientific journal

Regulatory Effect of Interleukin-4 in the Innate Inflammatory Response to Rhodococcus aurantiacus Infection in Mice
Yimin, Masashi Kohanawa, Songji Zhao, Minqi Li, Yuji Kuge, Nagara Tamaki, Masahiko Watanabe
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 35, 3, 222, 231, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Amoebal Endosymbiont Parachlamydia acanthamoebae Bn-9 Can Grow in Immortal Human Epithelial HEp-2 Cells at Low Temperature; An In Vitro Model System to Study Chlamydial Evolution
Chikayo Yamane, Tomohiro Yamazaki, Shinji Nakamura, Junji Matsuo, Kasumi Ishida, Sumire Yamazaki, Satoshi Oguri, Natsumi Shouji, Yasuhiro Hayashi, Mitsutaka Yoshida, Yimin, Hiroyuki Yamaguchi
PLOS ONE, 10, 2, e0116486, Feb. 2015, [Peer-reviewed]
English, Scientific journal

Staging liver fibrosis by analysis of non-linear normalization texture in gadolinium-enhanced magnetic resonance imaging.　　　　　　　　　　　　　　　
Mou W, Guo D, Liu H, Zhang p, Shao Y, Wang S, Yimin, Zheng L
Biomedical Physics & Engineering Express, 045012, 045012., 2015, [Peer-reviewed]
English, Scientific journal

Feature Selection Combined with Neural Network Structure Optimization for HIV-1 Protease Cleavage Site Prediction
Hui Liu, Xiaomiao Shi, Dongmei Guo, Zuowei Zhao, Yimin
BIOMED RESEARCH INTERNATIONAL, 2015, 263586, 2015, [Peer-reviewed]
English, Scientific journal

Histological Evidence of Increased Osteoclast Cell Number and Asymmetric Bone Resorption Activity in the Tibiae of Interleukin-6-Deficient Mice
Hongrui Liu, Wei Feng, Yimin, Jian Cui, Shengyu Lv, Tomoka Hasegawa, Bao Sun, Juan Li, Kimimitsu Oda, Norio Amizuka, Minqi Li
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 62, 8, 556, 564, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Contribution of Toll-Like Receptor 2 to the Innate Response against Staphylococcus aureus Infection in Mice
Yimin, Masashi Kohanawa, Songji Zhao, Michitaka Ozaki, Sanae Haga, Guangxian Nan, Yuji Kuge, Nagara Tamaki
PLoS ONE, 8, 9, e74287, 9, 13 Sep. 2013, [Peer-reviewed]
English, Scientific journal

Chlamydophila pneumoniae in human immortal Jurkat cells and primary lymphocytes uncontrolled by interferon-gamma
Kasumi Ishida, Takeru Kubo, Ayumi Saeki, Chikayo Yamane, Junji Matsuo, Yimin, Shinji Nakamura, Yasuhiro Hayashi, Miyuki Kunichika, Mitsutaka Yoshida, Kaori Takahashi, Itara Hirai, Yoshimasa Yamamoto, Ken-ichiro Shibata, Hiroyuki Yamaguchi
MICROBES AND INFECTION, 15, 3, 192, 200, Mar. 2013, [Peer-reviewed]
English, Scientific journal

Anoikis Induction and Inhibition of Peritoneal Metastasis of Pancreatic Cancer Cells by a Nuclear Factor-kappa B Inhibitor, (-)-DHMEQ
Masanori Sato, Kazuaki Nakanishi, Sanae Haga, Masato Fujiyoshi, Motoi Baba, Kazuhiro Mino, Yimin, Haruki Niwa, Hideki Yokoo, Kazuo Umezawa, Yoshihiro Ohmiya, Toshiya Kamiyama, Satoru Todo, Akinobu Taketomi, Michitaka Ozaki
ONCOLOGY RESEARCH, 21, 6, 333, 343, 2013, [Peer-reviewed]
English, Scientific journal

Ferrous Ferric Chloride Downregulates the Inflammatory Response to Rhodococcus aurantiacus Infection in Mice
Yimin, Huirong Tao, Masashi Kohanawa, Songji Zhao, Yuji Kuge, Nagara Tamaki
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 35, 12, 2214, 2223, Dec. 2012, [Peer-reviewed]
English, Scientific journal

Xanthohumol Prevents Atherosclerosis by Reducing Arterial Cholesterol Content via CETP and Apolipoprotein E in CETP-Transgenic Mice
Hiroshi Hirata, Yimin, Shuichi Segawa, Moeko Ozaki, Naoyuki Kobayashi, Tatsuro Shigyo, Hitoshi Chiba
PLOS ONE, 7, 11, e49415, Nov. 2012, [Peer-reviewed]
English, Scientific journal

A domino-like chlamydial attachment process: concurrent Parachlamydia acanthamoebae attachment to amoebae is required for several amoebal released molecules and serine protease activity
Yasuhiro Hayashi, Yimin, Junji Matsuo, Shinji Nakamura, Miyuki Kunichika, Mitsutaka Yoshida, Kaori Takahashi, Hiroyuki Yamaguchi
MICROBIOLOGY-SGM, 158, 6, 1607, 1614, Jun. 2012, [Peer-reviewed]
English, Scientific journal

A novel murine model for non-alcoholic steatohepatitis developed by combination of a high-fat diet and oxidized low-density lipoprotein
Yin Lin, Hiroaki Furumaki, Shiho Matsuoka, Toshihiro Sakurai, Masashi Kohanawa, Songji Zhao, Yuji Kuge, Nagara Tamaki, Hitoshi Chiba
LABORATORY INVESTIGATION, 92, 2, 265, 281, Feb. 2012, [Peer-reviewed]
English, Scientific journal

病原性クラミジアのリンパ球細胞への感染とIFN-gamma抵抗性
石田 香澄, 久保 剛流, 伊 敏, 松尾 淳司, 中村 眞二, 山口 博之
日本細菌学雑誌, 67, 1, 104, 104, 日本細菌学会, Feb. 2012
Japanese

Dynamic C-11-methionine PET analysis has an additional value for differentiating malignant tumors from granulomas: an experimental study using small animal PET
Songji Zhao, Yuji Kuge, Min Yi, Yan Zhao, Toshiyuki Hatano, Keiichi Magota, Ken-ichi Nishijima, Masashi Kohanawa, Nagara Tamaki
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 38, 10, 1876, 1886, Oct. 2011, [Peer-reviewed]
English, Scientific journal

肉芽腫・腫瘍におけるFMISO‐PETの有用性:小動物用PETを用いた基礎的検討　　　　　　　　　　　　　　　
趙松吉, 久下裕司, 趙芫, 伊敏, 波多野利行, 鈴木幸太郎, 孫田恵一, 西嶋剣一, 小華和柾志, 玉木長良
核医学, 47, 3, 373, 373, (一社)日本核医学会, Sep. 2010
Japanese

肉芽腫・腫瘍の鑑別診断におけるdynamic ¹¹C‐MET‐PETの有用性:小動物用PETを用いた基礎的検討　　　　　　　　　　　　　　　
趙松吉, 久下裕司, 伊敏, 趙芫, 竹内啓, 波多野利行, 鈴木幸太郎, 孫田惠一, 西嶋剣一, 小華和柾志, 玉木長良
核医学, 46, 3, 327, 327, (一社)日本核医学会, Sep. 2009
Japanese

Mutual modulation between interleukin-10 and interleukin-6 induced by Rhodococcus aurantiacus infection in mice
Yimin, Masashi Kohanawa, Michitaka Ozaki, Sanae Haga, Keiko Fujikawa, Songji Zhao, Yuji Kuge, Nagara Tamaki
MICROBES AND INFECTION, 10, 14-15, 1450, 1458, Nov. 2008, [Peer-reviewed]
English, Scientific journal

Usefulness of C-11-methionine for differentiating tumors from granulomas in experimental rat models: A comparison with F-18-FDG and F-18-FLT
Songji Zhao, Yuji Kuge, Masashi Kohanawa, Toshiyuki Takahashi, Yan Zhao, Min Yi, Kakuko Kanegae, Koh-ichi Seki, Nagara Tamaki
JOURNAL OF NUCLEAR MEDICINE, 49, 1, 135, 141, Jan. 2008, [Peer-reviewed]
English, Scientific journal

A regulatory effect of the balance between TNF-alpha and IL-6 in the granulomatous and inflammatory response to Rhodococcus aurantiacus infection in mice
Yimin, M Kohanawa
JOURNAL OF IMMUNOLOGY, 177, 1, 642, 650, Jul. 2006, [Peer-reviewed]
English, Scientific journal

Up-regulation of granulomatous inflammation in interleukin-6 knockout mice infected with Rhodococcus aurantiacus
Yimin, M Kohanawa, T Minagawa
IMMUNOLOGY, 110, 4, 501, 506, Dec. 2003, [Peer-reviewed]
English, Scientific journal

Lipopolysaccharide triggers invasive streptococcal disease in mice through a tumour necrosis factor-alpha-dependent mechanism
HY Diao, M Kohanawa, Yimin, F Nakajima, Y Sato, T Minagawa, A Nakane
IMMUNOLOGY, 105, 3, 344, 349, Mar. 2002, [Peer-reviewed]
English, Scientific journal

Role of T cells in granuloma formation induced by Rhodococcus aurantiacus is independent of their interferon-gamma production
Yimin, M Kohanawa, Y Sato, T Minagawa
JOURNAL OF MEDICAL MICROBIOLOGY, 50, 8, 688, 694, Aug. 2001, [Peer-reviewed]
English, Scientific journal

Suppression of late asthmatic response by low-dose oral administration of interferon-beta in the guinea pig model of asthma
Y Satoh, K Kasama, M Kuwabara, Yimin, HY Diao, H Nakajima, M Kohanawa, T Minagawa
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 19, 8, 887, 894, Aug. 1999, [Peer-reviewed]
English, Scientific journal

Movement disorders in encephalitis induced by Rhodococcus aurantiacus infection relieved by the administration of L-dopa and anti-T-cell antibodies
Y Min, M Asano, M Kohanawa, T Minagawa
IMMUNOLOGY, 96, 1, 10, 15, Jan. 1999, [Peer-reviewed]
English, Scientific journal

PARALYSIS CAUSED BY ACUTE MYELITIS IN THEILER MURINE ENCEPHALOMYELITIS VIRUS-STRAIN GD-VII INFECTION IS INDUCED BY CD4(+) LYMPHOCYTES INFILTRATING THE SPINAL-CORD
M KOHANAWA, M ASANO, Y MIN, T MINAGAWA, A NAKANE
JOURNAL OF GENERAL VIROLOGY, 76, 2251, 2256, Sep. 1995, [Peer-reviewed]
English, Scientific journal

脂肪毒性による肝細胞障害におけるヨモギの効果の検討　　　　　　　　　　　　　　　
伊敏, 神繁樹, 五十嵐元子, 津田弓子, 第40回和漢医薬学会学術学会抄録集, 2023

Staphylococcus aureus感染におけるTLR2欠損による感染防御反応の異常　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 第84回日本インターフェロン・サイトカイン学会学術学会抄録集, 2019

IL‐6遺伝子欠損マウスの骨組織における組織化学的解析
森谷康人, 森谷康人, 伊敏, 邱紫せん, 山本知真也, 鄭漢忠, 網塚憲生, 長谷川智香, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 124th, 162, 2019
Japanese

Toll like receptor(TLR)2遺伝子欠損マウスの破骨細胞における組織学的検索
邱紫せん, 伊敏, 山本知真也, 李敏啓, 森本景之, 網塚憲生, 長谷川智香, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 124th, 142, 2019
Japanese

Toll like receptor(TLR)2遺伝子欠損マウスの破骨細胞における組織学的検索
邱紫せん, 邱紫せん, 長谷川智香, 本郷裕美, 山本知真也, 李敏啓, 伊敏, 網塚憲生, 日本骨粗鬆症学会雑誌, 4, Suppl.1, 294, 26 Sep. 2018
Japanese

Staphylococcus aureus感染におけるTLR2欠損による感染防御反応の異常　　　　　　　　　　　　　　　
伊敏, 第92回日本感染症学会学術会議抄録集, 2018

NASHモデルマウスにおけるマガキ抽出物の肝臓保護作用　　　　　　　　　　　　　　　
布田 博敏, 渡邉 貢, 岡部 浩昭, 上甲 紗愛, 三浦 佑介, 惠 淑萍, 伊 敏, 濱岡 直裕, 三木 恵美子, 千葉 仁志, 日本未病システム学会学術総会抄録集, 23回, 115, 115, Oct. 2016
(一社)日本未病システム学会, Japanese

R. aurantiacus感染マウスにおけるTLR2欠損による感染重症化　　　　　　　　　　　　　　　
伊敏, 第90回日本感染症学会学術会議抄録集, 2016

Role of initial cytokines in the formation and downregulation of hepatic granulomas in the inflammatory response to Rhodococcus aurantiacus infection in mice　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 第88回日本細菌学会学術会議抄録集, Mar. 2015

伊敏　　　　　　　　　　　　　　　
第80回日本インターフェロン・サイトカイン学会学術会議抄録集, 2015

Rhodococcus aurantiacus感染におけるIL-4の自然免疫応答への寄与　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 第79回日本インターフェロン・サイトカイン学会学術会議抄録集, Jul. 2014

肉芽腫・腫瘍の鑑別診断における18F-FMISO低酸素イメージング 小動物用PETを用いたラットモデルにおけるFDGとの比較　　　　　　　　　　　　　　　
趙 松吉, 久下 裕司, 趙 芫, 伊 敏, 于 聞文, 西嶋 剣一, 孫田 恵一, 小華和 柾志, 玉木 長良, JSMI Report, 7, 2, 104, 104, May 2014
日本分子イメージング学会, Japanese

腫瘍・肉芽腫における18F-FMISO集積の特徴 18F-FDG及び組織学的比較
趙 松吉, 伊 敏, 趙 芫, 西嶋 剣一, 村上 正紘, 于 聞文, チョドリ・ヌスラット・ファティマ, 孫田 恵一, 小華和 柾志, 玉木 長良, 久下 裕司, 核医学, 49, 3, S195, S195, Aug. 2012
(一社)日本核医学会, Japanese

A downregulatory effect of Pairogen, a heath beverage, on inflammatory response to Rhodococcus aurantiacus infection in mice　　　　　　　　　　　　　　　
伊敏, 陶恵栄, 小華和柾志, 第85回日本細菌学会学術会議抄録集, Mar. 2012

The effects of TLR2 and CD36 in Staphylococcus aureus infection　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 第83回日本細菌学会学術会議抄録集, Mar. 2010

apoE‐richHDLの分離とHDL測定試薬の反応性
高橋祐司, 高橋祐司, 古牧宏啓, 櫻井俊宏, 神繁樹, 惠淑萍, 伊敏, 千葉仁志, 臨床化学, 38, Suppl.1, 147, 147, 31 Jul. 2009
(一社)日本臨床化学会, Japanese

apoE‐rich HDLと各種沈殿試薬の反応性について
古牧宏啓, 高橋祐司, 櫻井俊宏, 伊敏, 惠淑萍, 神繁樹, 千葉仁志, 臨床化学, 38, 146, 31 Jul. 2009
Japanese

動脈硬化惹起性リポ蛋白測定系の若年健常者における検討
鈴木純子, 櫻井俊宏, 高橋祐司, 惠淑萍, 神繁樹, 伊敏, 千葉仁志, 臨床化学, 38, 151, 31 Jul. 2009
Japanese

酸化LDLによるCD36の発現変化
伊敏, 櫻井俊宏, 松岡志保, 須藤祐, 古牧宏啓, 神繁樹, 恵淑萍, 千葉仁志, 臨床病理, 57, 175, 28 Jul. 2009
Japanese

CD36欠損者の長期フルクトース摂取による代謝変化—脂肪負荷試験による検討—
高橋祐司, 鈴木純子, 櫻井俊宏, 神繁樹, 惠淑萍, 伊敏, 千葉仁志, 臨床病理, 57, 176, 28 Jul. 2009
Japanese

CD36欠損者の長期フルクトース摂取による代謝変化―糖負荷試験による検討―
櫻井俊宏, 鈴木純子, 高橋祐司, 神繁樹, 惠淑萍, 伊敏, 千葉仁志, 臨床病理, 57, 175, 28 Jul. 2009
Japanese

CD36欠損者の長期フルクトース摂取が,H‐MRS測定法による骨格筋細胞内脂質量に及ぼす影響
鈴木純子, 山本徹, 辻真太郎, 櫻井俊宏, 高橋祐司, 神繁樹, 惠淑萍, 伊敏, 千葉仁志, 臨床病理, 57, 176, 28 Jul. 2009
Japanese

Rhodococcus aurantiacus感染におけるIL-10とIL-6の相互制御　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 第81回日本細菌学会学術会議抄録集, Mar. 2008

IL-6遺伝子欠損マウスにおけるIL-10の代償性増加　　　　　　　　　　　　　　　
伊敏, 第72回日本インターフェロン・サイトカイン学会学術会議抄録集, 2007

TNF-α/IL-6バランスによるマウスの肉芽腫性炎症反応の制御　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 第71回日本インターフェロン・サイトカイン学会学術会議抄録集, 2006

サイトカインのバランスによる肉芽腫性炎症反応の制御　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 第79回日本細菌学学術会議抄録集, 2006

感染誘導マウス肉芽腫形成における樹状細胞の役割　　　　　　　　　　　　　　　
伊敏, 新開大史, 松本美佐子, 小華和柾志, 瀬谷司, 第78回日本細菌学会学術会議抄録集, 2005

TNF-αノックアウトマウスにおける肉芽腫の形成　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 瀬谷司, 第69回日本インターフェロン・サイトカイン学会学術会議抄録集, 2004

Rhodococcus aurantiacus感染により誘導されたマウスの肉芽腫形成におけるTNF-αの役割　　　　　　　　　　　　　　　
伊敏, 第77回日本細菌学会学術会議抄録集, 2004

TNF-αノックアウトマウスにおけるRhodococcus aurantiacus感染致死　　　　　　　　　　　　　　　
伊敏, 皆川知紀, 第76回日本細菌学会学術会議抄録集, 2003

Infectious granuloma formation in IL-6 KO mice
YI MIN, MINAGAWA TOMONORI, KOHANAWA MASASHI, 無菌生物 = Japanese journal of germfree life and gnotobiology, 32, 2, 115, 115, 01 Dec. 2002
Japanese

マウスの肉芽腫形成におけるIL-6の抑制効果　　　　　　　　　　　　　　　
伊敏, 皆川知紀, 小華和柾志, 佐藤雄一郎, 第75回日本細菌学会学術会議抄録集, 2002

IFN-gamma knockout mice, IL-4 knockout miceおよびIL-6 knockout miceにおけるRhodococcus aurantiacus感染により誘導される肉芽腫形成。　　　　　　　　　　　　　　　
皆川知紀, 伊敏, 乳酸菌研究会報告書, 1, 18, 2002

感染性肉芽腫形成におけるIL-6の産生動態　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 佐藤雄一郎, 皆川知紀, 第74回日本細菌学会学術会議抄録集, 2001

IL-6 ノックアウトマウスにおける肉芽腫形成　　　　　　　　　　　　　　　
皆川知紀, 伊敏, 乳酸菌研究会報告書, 1, 13, 2001

IL-6ノックアウトマウスにおける肉芽腫の形成　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 中島寛文, 佐藤雄一郎, 皆川知紀, 第73回日本細菌学会学術会議抄録集, 2000

IFN-γノックアウトマウスおよびIL-4ノックアウトマウスにおける肉芽腫形成の比較　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 佐藤雄一郎, 皆川知紀, 第72回日本細菌学会学術会議抄録集, 1999

IFN-γノックアウトマウスにおける肉芽腫形成の抑制機序　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 佐藤雄一郎, 皆川知紀, 第71回日本細菌学会学術会議抄録集, 1998

IFN-γノックアウトマウスにおける肉芽腫形成　　　　　　　　　　　　　　　
伊敏, 小華和柾志, 佐藤雄一郎, 皆川知紀, 第17回日本サルコイドーシス学会学術会議抄録集, 1997

感染症におけるサイトカインとTh細胞　　　　　　　　　　　　　　　
インターフェロン その研究の歩みと臨床応用への可能性, 1998

Regulatory action of toll-like receptor 2 in a non-alcoholic steatohepatitis mouse model　　　　　　　　　　　　　　　
伊 敏
5th Annual Meeting of the International Cytokine and interferon Society, Oct. 2017, English, Poster presentation
[International presentation]

Function of Toll-like receptor 2 in the murine inflammatory response to Rhodococcus aurantiacus infection　　　　　　　　　　　　　　　
伊 敏
4th Annual Meeting of the International Cytokine and interferon Society, San Francisco, Oct. 2016, English, Poster presentation
[International presentation]

Regulatory effect of interleukin-4 in the innate immune and inflammatory responses to Rhodococcus aurantiacus infection in mice　　　　　　　　　　　　　　　
伊 敏
2th Annual Meeting of the International Cytokine and Interferon Society, Oct. 2014, English, Poster presentation
[International presentation]

Down-regulatory effect of Pairogen, a health drink, on inflammatory response to Rhodococcus aurantiacus infection in mice　　　　　　　　　　　　　　　
伊 敏
10th Joint Meeting of International Cytokine Society and International Society for Interferon and Cytokine Research, Sep. 2012, English, Poster presentation
[International presentation]

The roles of TLR2 and CD36 in response to Staphylococcus aureus in vivo　　　　　　　　　　　　　　　
伊敏
8th Joint Meeting of International Cytokine Society and International Society for Interferon and Cytokine Research, Oct. 2010, English, Poster presentation
[International presentation]

CD36 function in a novel non-alcoholic steatohepatitis model in mice　　　　　　　　　　　　　　　
Yimin
14th international congress of immunology, Aug. 2010, English, Poster presentation
[International presentation]

Interaction between IL-10 and IL-6 in mice infected with Rodococcus aurantiacus.　　　　　　　　　　　　　　　
Yimin
4th Congress of the Federation of Immunology societies of Asia-Oceania, Oct. 2008, English, Poster presentation
[International presentation]

A regulatory effect of the balance between tumor necrosis factor-alpha and interleukine-6 in inflammatory response.　　　　　　　　　　　　　　　
Yimin
6th Joint Meeting of International Cytokine Society, Aug. 2006, English, Poster presentation
[International presentation]

生物学実習　　　　　　　　　　　　　　　
北海道大学

細菌学　　　　　　　　　　　　　　　
北海道大学

初級中国語演習　　　　　　　　　　　　　　　
北海道大学

中級英語演習　　　　　　　　　　　　　　　
北海道大学

免疫学　　　　　　　　　　　　　　　
北海道大学

2023 - Present
和漢医薬学会　　　　　　　　　　　　　　　

2016 - Present
THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES　　　　　　　　　　　　　　　

2004 - Present
日本インターフェロンサイトカイン学会　　　　　　　　　　　　　　　

1997 - Present
北海道医学会　　　　　　　　　　　　　　　

自然免疫異常によるグラム陽性菌感染症の重症化機序についての解析　　　　　　　　　　　　　　　
基盤研究（C）
Apr. 2017 - Mar. 2020
伊敏
日本学術振興会, Principal investigator, Competitive research funding

細胞死による積極的な肝機能維持・再生制御機構の解明と臨床応用に向けた研究　　　　　　　　　　　　　　　
挑戦的萌芽研究
Apr. 2014 - Mar. 2015
伊敏
日本学術振興会, Principal investigator, Competitive research funding

パイロゲンの免疫防御反応への制御について　　　　　　　　　　　　　　　
交流助成金
Apr. 2012 - Mar. 2013
伊敏
伊藤医薬学術交流財団, Principal investigator, Competitive research funding

炎症性サイトカインと抗炎症性サイトカインのバランスの制御機構の解明に関する研究　　　　　　　　　　　　　　　
学術研究助成奨励金
Apr. 2008 - Mar. 2009
伊敏
日本ワックスマン財団, Principal investigator, Competitive research funding

サルコイドーシス病因の細菌学的検索と免疫療法の確立
科学研究費補助金(基盤研究(B))
1996 - 1998
皆川 知紀, 佐藤 雄一郎, 小華和 柾志, 伊 敏
サルコイドーシスは、非壊死性肉芽腫が各種臓器に形成される原因不明全身性炎症性疾患である。これまで患者病的材料を用いて、病因の追求及び患者の免疫学的側面をサイトカインネットワークから追求してきた。一方で、マウスを用いた実験的肉芽腫形成モデルについて追求してきた。1.病因については結核説、プロピオニバクテリウム説が現在の所有力であるが、それらのいずれかが単独で発症させる可能性は少なく、むしろ多因子の背景があるところへある種の感染が引き金となつている可能性が強い。我々は非病原性であり、土壌細菌(環境細菌)出来でもあるRhodococcus aurantiacusも原因の一つになりえるとの立場から検索したが、患者リンパ節からは有意には分離されなかった。2.サルコイドーシス患者の免疫学的特性としてツベルクリン反応が陰性であるアネルギーがあるが、それは全身性の肉芽腫反応の結果であり、免疫不全を意味しない。患者リンパ節の肉芽腫病変よりIL-1、TNFα、IFN-γが検出されるが中でもIFN―γは血中レベル、病勢と相関している事を明らかにした。免疫組織染色などからもサルコイドーシス肉芽腫形成はThlタイプの反応の結果であることを明らかにした。3.Rhodococcusをマウスに静注すると肝臓、牌臓、肺などに典型的な非壊死性肉芽腫が形成されるが、これもサルコイドーシス同様Th1タイプの反応の結...
文部科学省, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 08457084