Mar. 2023, 日本水環境学会, 学生ポスター発表賞（ライオン賞）　　　　　　　　　　　　　　　
DHSろ床を用いたPET原料製造廃水処理UASB反応器の後段処理技術の開発
髙井麻帆;中屋佑紀;佐藤久;相沢智康;大西裕季;熊木康裕;成廣隆;黒田恭平

Mar. 2023, 日本水環境学会, 学生ポスター発表賞（ライオン賞）　　　　　　　　　　　　　　　
緑色蛍光タンパク質遺伝子導入大腸菌を用いた活性汚泥フロックの大腸菌吸着部位の解明
石崎翔大;石塚祐介;樋口宏介;押木守;中屋佑紀;佐藤久

Nov. 2022, 日本微生物生態学会, 優秀ポスター賞　　　　　　　　　　　　　　　
金ナノ粒子プローブを用いた環境中微生物の簡易核酸分析法の開発
中島 芽梨;中屋佑紀;黒田恭平;成廣隆;佐藤久

Nov. 2022, 日本微生物生態学会, 優秀ポスター賞　　　　　　　　　　　　　　　
PCRフリーの金ナノ粒子プローブ法を用いた廃水処理汚泥中のPatescibacteriaとメタン生成アーキアの迅速定量の試み
半田 久純;中島 芽梨;黒田 恭平;成廣 隆;佐藤 久

Aug. 2022, 日本下水道協会, 下水道研究発表会口頭発表優秀賞　　　　　　　　　　　　　　　
活性汚泥診断の自動化に向けたフロック定量化技術の開発
間嶋義喜;野田周平;松代武士;平岡由紀夫;佐藤久

Mar. 2021, 日本水環境学会, 年会優秀発表賞（クリタ賞）　　　　　　　　　　　　　　　
光導波路分光装置を用いた下水処理プロセスの微生物モニタリング
中島芽梨;佐藤久平野麗子;高橋浩三

May 2020, Japan Sewage Works Association, Selected paper　　　　　　　　　　　　　　　
特定酵素蛍光基質を用いた下水中の大腸菌群の簡易迅速測定法の開発
佐藤久;津田収;菊地凱;平野麗子

Mar. 2020, 日本水環境学会, 年会学生ポスター発表特別賞(ライオン特別賞)　　　　　　　　　　　　　　　
ディープラーニングを用いた活性汚泥フロックの認識
柏本ゆかり;佐藤久

Jan. 2020, 化学系学協会北海道支部, 優秀講演賞（ポスター部門）　　　　　　　　　　　　　　　
金ナノ粒子を用いた細菌の16S rRNAの比色分析
中島芽梨・北大;平野麗子・セルスペクト株式会社;佐藤久・北大

Dec. 2019, 土木学会, 環境工学研究フォーラム優秀ポスター発表賞　　　　　　　　　　　　　　　
金ナノ粒子を用いた 16S rRNA をターゲットとした細菌の比色分析
中島芽梨

Dec. 2019, 土木学会, 土木学会環境工学研究フォーラム環境技術・プロジェクト賞　　　　　　　　　　　　　　　
ディープラーニングを用いた画像認識による活性汚泥中微生物の検出
柏本ゆかり

Jul. 2019, 令和1年7月 The WET Excellent Presentation Award at WET2019（松永）, The WET Excellent Presentation Award at WET2019　　　　　　　　　　　　　　　
松永 光司

Dec. 2018, 土木学会, 土木学会環境工学研究フォーラム環境技術・プロジェクト賞　　　　　　　　　　　　　　　
三価と五価のヒ素を分別定量可能な新規光学的簡易バイオセンサーの開発
松永光司

Jul. 2018, 下水道研究発表会, ポスター発表優秀賞　　　　　　　　　　　　　　　
β-グルクロニダーゼを用いた新規簡易大腸菌数測定法の開発
菊地凱

Mar. 2018, 日本水環境学会, 年会優秀発表賞（クリタ賞）　　　　　　　　　　　　　　　
DNAアプタマーを用いた簡易ヒ素分析法の開発
松永光司

Nov. 2017, 土木学会, 土木学会環境工学研究フォーラム環境技術・プロジェクト賞　　　　　　　　　　　　　　　
DNAアプタマーを用いた簡易ヒ素分析法の開発
松永光司

Nov. 2017, 土木学会, 土木学会環境工学研究フォーラム優秀ポスター発表賞　　　　　　　　　　　　　　　
菊地凱

Oct. 2017, 土木学会, 環境工学研究フォーラム環境技術・プロジェクト賞　　　　　　　　　　　　　　　
特定酵素蛍光基質を用いた簡易大腸菌数測定法の開発
片寄由貴

Mar. 2017, 水環境学会, 年会学生ポスター発表賞（ライオン賞）　　　　　　　　　　　　　　　
山口拓郎

Mar. 2017, 水環境学会, 年会優秀発表賞（クリタ賞）　　　　　　　　　　　　　　　
奥山優

Mar. 2015, 水環境学会, 年会優秀発表賞（クリタ賞）　　　　　　　　　　　　　　　
山田健太

Sep. 2014, 水環境学会, 博士研究奨励賞（オルガノ賞）　　　　　　　　　　　　　　　
羽深 昭

Mar. 2014, 水環境学会, 年会優秀発表賞（クリタ賞）　　　　　　　　　　　　　　　
坂槙有紀恵

Jan. 2014, 土木学会, 環境工学フォーラム論文奨励賞　　　　　　　　　　　　　　　
羽深 昭

Jan. 2013, The Bulletin of the Chemical Society of Japan, Selected paper　　　　　　　　　　　　　　　
BODIPY-based ratiometric fluoroionophores with bidirectional spectral shifts for the selective recognition of heavy metal ions
SATOH Hisashi

Jun. 2012, Best Presentation Award at WET2012　　　　　　　　　　　　　　　
佐藤 久

Nov. 2011, 土木学会環境工学研究フォーラム優秀ポスター発表賞　　　　　　　　　　　　　　　
Japan

Oct. 2011, Best Student Award　　　　　　　　　　　　　　　

Jun. 2010, WET2010 Best Presentation Award　　　　　　　　　　　　　　　

Jan. 2008, IWA, Paper Award at IWA Biofilm Technologies Conference　　　　　　　　　　　　　　　

May 2006, 第5回インテリジェント・コスモス奨励賞　　　　　　　　　　　　　　　
佐藤 久

Jun. 2005, 日本水環境学会論文奨励賞（廣瀬賞）　　　　　　　　　　　　　　　
佐藤 久

Nov. 2004, 土木学会環境工学研究フォーラム優秀ポスター発表賞　　　　　　　　　　　　　　　
佐藤 久

Sep. 2003, 土木学会年次学術講演会優秀講演者賞　　　　　　　　　　　　　　　
佐藤 久

Sep. 1997, 土木学会年次学術講演会優秀講演者賞　　　　　　　　　　　　　　　
佐藤 久

Solid-phase-fluorescence intensity of clay–fulvic acid complexes: differences based on amount of fulvic acid adsorbed and examination of correction by using the Kubelka–Munk function
Yuki Nakaya, Takashi Hirose, Ryuichi Tamori, Nobuhide Fujitake, Satoru Nakashima, Hiroshi Yamamura, Hisashi Satoh
Analytical Sciences, Mar. 2026, [Peer-reviewed]
English, Scientific journal

Resuscitation, regrowth, and release of bacteria in biofilm in a river receiving chlorinated reclaimed water
Mohomed N.M. Shayan, Yuna Tanaka, Ayaka Shinji, Taisei Nakayama, Reiko Hirano, Kyohei Kuroda, Takashi Narihiro, Yuki Nakaya, Hisashi Satoh
Water Research X, 100524, 100524, Elsevier BV, Mar. 2026, [Peer-reviewed]
English, Scientific journal

Monitoring Sexually Transmitted Infection-Associated Pathogens in Municipal Wastewater in Japan
Made Sandhyana Angga, Vu Duc Canh, Yuki Nakaya, Hisashi Satoh, Hiroyuki Katayama, Masaaki Kitajima
ACS ES&T Water, 5, 12, 7437, 7444, American Chemical Society (ACS), 11 Nov. 2025
Scientific journal

Microbiology of Anaerobic Wastewater Treatment Processes Treating Purified Terephthalic Acid and Related Aromatic Compounds
Kyohei Kuroda, Meri Nakajima, Rino Isshiki, Hisashi Satoh, Takashi Narihiro
Urban Watershed Microbiology, Volume 2, 1167, 1203, Springer Nature Switzerland, 12 Oct. 2025
In book

Solid-phase fluorescence excitation-emission matrix spectroscopy of soil, fulvic acid fractions, and clay mineral complexes: Evidence from red shift of fluorescence maxima associated with aggregation
Yuki Nakaya, Takashi Hirose, Ryuichi Tamori, Nobuhide Fujitake, Satoru Nakashima, Hiroshi Yamamura, Hisashi Satoh
Environmental Research, 121900, 121900, Elsevier BV, May 2025, [Peer-reviewed]
English, Scientific journal

Effects of snowmelt runoff on bacterial communities and antimicrobial resistance gene concentrations in an urban river in a cold climate region
Mohomed N. M. Shayan, Takeshiro Onodera, Reiko Hirano, Kyohei Kuroda, Takashi Narihiro, Yuki Nakaya, Hisashi Satoh
Environmental Science and Pollution Research, Springer Science and Business Media LLC, 05 Mar. 2025
Scientific journal

Minisyncoccus archaeiphilus gen. nov., sp. nov., a mesophilic, obligate parasitic bacterium and proposal of Minisyncoccaceae fam. nov., Minisyncoccales ord. nov., Minisyncoccia class. nov. and Minisyncoccota phyl. nov. formerly referred to as Candidatus Patescibacteria or candidate phyla radiation.
Meri Nakajima, Ryosuke Nakai, Yuga Hirakata, Kengo Kubota, Hisashi Satoh, Masaru K Nobu, Takashi Narihiro, Kyohei Kuroda
International journal of systematic and evolutionary microbiology, 75, 2, Feb. 2025, [International Magazine]
English, Scientific journal, In the domain Bacteria, one of the largest, most diverse and environmentally ubiquitous phylogenetic groups, Candidatus Patescibacteria (also known as candidate phyla radiation/CPR), remains poorly characterized, leaving a major knowledge gap in microbial ecology. We recently discovered a novel cross-domain symbiosis between Ca. Patescibacteria and Archaea in highly purified enrichment cultures and proposed Candidatus taxa for the characterized species, including Ca. Minisyncoccus archaeophilus and the corresponding family Ca. Minisyncoccaceae. In this study, we report the isolation of this bacterium, designated strain PMX.108T, in a two-strain co-culture with a host archaeon, Methanospirillum hungatei strain DSM 864T (JF-1T), and hereby describe it as the first representative species of Ca. Patescibacteria. Strain PMX.108T was isolated from mesophilic methanogenic sludge in an anaerobic laboratory-scale bioreactor treating synthetic purified terephthalate- and dimethyl terephthalate-manufacturing wastewater. The strain could not grow axenically and is obligately anaerobic and parasitic, strictly depending on M. hungatei as a host. The genome was comparatively large (1.54 Mbp) compared to other members of the clade, lacked some genes involved in the biosynthesis pathway and encoded type IV pili-related genes associated with the parasitic lifestyle of ultrasmall microbes. The G+C content of the genomic DNA was 36.6 mol%. Here, we report the phenotypic and genomic properties of strain PMX.108T; we propose Minisyncoccus archaeiphilus gen. nov., sp. nov. to accommodate this strain. The type strain of the species is PMX.108T (=JCM 39522T). We also propose the associated family, order, class and phylum as Minisyncoccaceae fam. nov. Minisyncoccales nov., Minisyncoccia class. nov. and Minisyncoccota phyl. nov. within the bacterial kingdom Bacillati.

Feasibility study for the treatment of Palm Oil Mill Effluent (POME) using electrocoagulation method in Sri Lanka
Wasala M.K.R.T.W. Bandara, B.M.L.A. Basnayake, M.N.M. Shayan, I.M.V.U. Illangakoon, N.A.S. Nimanthika, K.H. Thanushka, H. Satoh
Desalination and Water Treatment, 321, 101054, 101054, Elsevier BV, Jan. 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Quantification of morphological characteristics and filamentous bacteria in activated-sludge flocs through quantitative image-analysis techniques incorporating image-processing software and U-Net deep-learning framework
Uthpala Kaushalya, Yuki Nakaya, Shota Ishizaki, Kai Sugino, Reiko Hirano, Hisashi Satoh
Journal of Water Process Engineering, Jan. 2025
English, Scientific journal

Introduction of Plug-flow Anaerobic Sludge Blanket (PASB) reactor for wastewater treatment
Wasala M.K.R.T.W. Bandara, Mohomed Shayan, R.M.W.C. Thennakoon, P.A.A. Kumudumali, Hisashi Satoh
Desalination and Water Treatment, 319, 100439, Elsevier BV, Jul. 2024, [Peer-reviewed], [Corresponding author]
Scientific journal

Physicochemical parameters affecting the adhesion of ciprofloxacin-resistant Escherichia coli to activated sludge.
Yuki Nakaya, Natsumi Nagahashi, Reiko Hirano, Yusuke Ishizuka, Hisashi Satoh
Water science and technology : a journal of the International Association on Water Pollution Research, 89, 9, 2457, 2467, May 2024, [International Magazine]
English, Scientific journal, To investigate the physicochemical conditions necessary to stably remove antibiotic-resistant bacteria (ARB) via contact with activated sludge (AS), the adhesion of ciprofloxacin (CIP)-resistant and -susceptible Escherichia coli to AS was simulated by contact tests in the laboratory. The CIP-resistant E. coli and susceptible E. coli were removed by a 3 log smaller concentration by a 5 h contact test at maximum. Considering the hydraulic retention time of a reaction tank (∼5 h) and step-feeding operation, we considered the removal rate of E. coli in the current simulated contact test to be in agreement with the actual situation where 1-2 log concentrations of E. coli were reported to be removed from an AS reaction tank. With the increase in the AS concentration and/or dissolved oxygen, the removal rate of E. coli increased. The removal rate of CIP-resistant E. coli was greater than that of susceptible E. coli under all experimental conditions. Although the mechanism by which CIP-resistant E. coli preferably adhered to AS was not clearly understood in detail, finding optimum conditions under which bacteria, including ARB, were efficiently removed by the AS process may be possible.

Development of an internal two-stage upflow anaerobic reactor integrating biostimulation strategies to enhance the degradation of aromatic compounds in wastewater from purified terephthalic acid and dimethyl terephthalate manufacturing processes
Kyohei Kuroda, Maho Takai, Takeo Sekiguchi, Tomoya Ikarashi, Hazuki Kurashita, Meri Nakajima, Masaru K. Nobu, Masashi Hatamoto, Takashi Yamaguchi, Yuki Nakaya, Hisashi Satoh, Masahito Yamauchi, Masayoshi Yamada, Takashi Narihiro
Water Research, 258, 121762, 121762, Elsevier BV, May 2024, [International Magazine]
English, Scientific journal, In this study, we aimed to establish high-rate biological treatment of purified terephthalic acid (PTA) and dimethyl terephthalate (DMT) wastewater that minimizes the inhibitory effects of high concentration benzoate and acetate. To achieve this, we developed a novel bioreactor system and biostimulation strategy. An internal two-stage upflow anaerobic (ITUA) reactor was operated with (i) a packed bed containing green tuff medium underlying (ii) a compartment seeded with anaerobic granular sludge. Ethylene glycol was amended to stimulate syntrophic interactions. Continuous operation of the system for 1,026 days achieve an organic removal rate of 11.0 ± 0.6 kg COD/m3/d. The abundance of aromatic degraders significantly increased during operation. Thus, we successfully developed a high-rate treatment system to treat wastewater from the PTA/DMT manufacturing processes by activating syntrophs in an ITUA reactor.

Microbiological insights into anaerobic phenol degradation mechanisms and bulking phenomenon in a mesophilic upflow anaerobic sludge blanket reactor in long-term operation
Kyohei Kuroda, Ryota Maeda, Futaba Shinshima, Kampachiro Urasaki, Kengo Kubota, Masaru K. Nobu, Taro Q.P. Noguchi, Hisashi Satoh, Masahito Yamauchi, Takashi Narihiro, Masayoshi Yamada
Water Research, 253, 121271, 121271, Elsevier BV, Feb. 2024, [International Magazine]
English, Scientific journal, In this study, a long-term operation of 2,747 days was conducted to evaluate the performance of the upflow anaerobic sludge blanket (UASB) reactor and investigated the degradation mechanisms of high-organic loading phenol wastewater. During the reactor operation, the maximum chemical oxygen demand (COD) removal rate of 6.1 ± 0.6 kg/m3/day under 1,680 mg/L phenol concentration was achieved in the mesophilic UASB reactor. After a significant change in the operating temperature from 24.0 ± 4.1 °C to 35.9 ± 0.6 °C, frequent observations of floating and washout of the bloated granular sludge (novel types of the bulking phenomenon) were made in the UASB reactor, suggesting that the change in operating temperature could be a trigger for the bulking phenomenon. Through the metagenomic analysis, phenol degradation mechanisms were predicted that phenol was converted to 4-hydroxybenzoate via two possible routes by Syntrophorhabdaceae and Pelotomaculaceae bacteria. Furthermore, the degradation of 4-hydroxybenzoate to benzoyl-CoA was carried out by members of Syntrophorhabdaceae and Smithellaceae. In the bulking sludge, a predominant presence of Nanobdellota, belonging to DPANN archaea, was detected. The metagenome-assembled genome of the Nanobdellota lacks many biosynthetic pathways and has several genes for the symbiotic lifestyle such as trimeric autotransporter adhesin-related protein. Furthermore, the Nanobdellota have significant correlations with several methanogenic archaea that are predominantly present in the UASB reactor. Considering the results of this study, the predominant Nanobdellota may negatively affect the growth of the methanogens through the parasitic lifestyle and change the balance of microbial interactions in the granular sludge ecosystem.

Collaborative metabolisms of urea and cyanate degradation in marine anammox bacterial culture
Mamoru Oshiki, Emi Morimoto, Kanae Kobayashi, Hisashi Satoh, Satoshi Okabe
ISME Communications, 4, 1, ycad007, Oxford University Press (OUP), 01 Jan. 2024, [International Magazine]
English, Scientific journal, Abstract

Anammox process greatly contributes to nitrogen loss occurring in oceanic oxygen minimum zones (OMZs), where the availability of NH4+ is scarce as compared with NO2−. Remineralization of organic nitrogen compounds including urea and cyanate (OCN−) into NH4+ has been believed as an NH4+ source of the anammox process in oxygen minimum zones. However, urea- or OCN−- dependent anammox has not been well examined due to the lack of marine anammox bacterial culture. In the present study, urea and OCN− degradation in a marine anammox bacterial consortium were investigated based on 15N-tracer experiments and metagenomic analysis. Although a marine anammox bacterium, Candidatus Scalindua sp., itself was incapable of urea and OCN− degradation, urea was anoxically decomposed to NH4+ by the coexisting ureolytic bacteria (Rhizobiaceae, Nitrosomonadaceae, and/or Thalassopiraceae bacteria), whereas OCN− was abiotically degraded to NH4+. The produced NH4+ was subsequently utilized in the anammox process. The activity of the urea degradation increased under microaerobic condition (ca. 32–42 μM dissolved O2, DO), and the contribution of the anammox process to the total nitrogen loss also increased up to 33.3% at 32 μM DO. Urea-dependent anammox activities were further examined in a fluid thioglycolate media with a vertical gradient of O2 concentration, and the active collaborative metabolism of the urea degradation and anammox was detected at the lower oxycline (21 μM DO).

Growth of the Nitrosomonas europaea cells in the biofilm and planktonic growth mode: Responses of extracellular polymeric substances production and transcriptome
Mamoru Oshiki, Takahiro Saito, Yuki Nakaya, Hisashi Satoh, Satoshi Okabe
Journal of Bioscience and Bioengineering, 136, 6, 430, 437, Elsevier BV, Nov. 2023, [Domestic magazines]
English, Scientific journal, Nitrosomonas europaea, an aerobic ammonia oxidizing bacterium, is responsible for the first and rate-limiting step of the nitrification process, and their ammonia oxidation activities are critical for the biogeochemical cycling and the biological nitrogen removal of wastewater treatment. In the present study, N. europaea cells were cultivated in the inorganic or organic media (the NBRC829 and the nutrient-rich, NR, media, respectively), and the cells proliferated in the form of planktonic and biofilm in those media, respectively. The N. europaea cells in the biofilm growth mode produced larger amounts of the extracellular polymeric substances (EPS), and the composition of the EPS was characterized by the chemical analyses including Fourier transform infrared spectroscopy (FT-IR) and 1H-nuclear magnetic resonance (NMR) measurements. The RNA-Seq analysis of N. europaea in the biofilm or planktonic growth mode revealed that the following gene transcripts involved in central nitrogen metabolisms were abundant in the biofilm growth mode; amo encoding ammonia monooxygenase, hao encoding hydroxylamine dehydrogenase, the gene encoding nitrosocyanine, nirK encoding copper-containing nitrite reductase. Additionally, the transcripts of the pepA and wza involved in the bacterial floc formation and the translocation of EPS, respectively, were also abundant in the biofilm-growth mode. Our study was first to characterize the EPS production and transcriptome of N. europaea in the biofilm and planktonic growth mode.

A simple and rapid method for detecting fecal pollution in urban rivers by measuring the intrinsic β-D-glucuronidase activity of Escherichia coli
Mohomed N.M. Shayan, Yuna Tanaka, Reiko Hirano, Yuki Nakaya, Hisashi Satoh
Water Research, 246, 120689, 120689, Elsevier BV, Nov. 2023, [International Magazine]
English, Scientific journal, As urban rivers are domestic, industrial, and agricultural water resources, fecal pollution poses human health and environmental risks. In this study, we developed a simple and rapid method to detect fecal pollution in urban rivers. Water samples were mixed with liquid medium, including a fluorescent substrate and fluorescence intensity (F.I.) was measured using a microplate reader to determine Escherichia coli (E. coli) β-D-glucuronidase (GUS) activity instead of E. coli concentration. GUS activities measurements in pure E. coli cultures revealed that E. coli incubated with a GUS substrate accumulated GUS enzymes in their cells, whereas those incubated without a GUS substrate did not. The increase in GUS activity corresponded to the proliferation of E. coli and the GUS activity increased linearly even during the lag growth phase of E. coli, indicating the presence of intrinsic GUS (iGUS) in E. coli cells before incubation. iGUS activity persisted at 81 % in the chlorinated samples, even though the E. coli concentration was reduced by a factor of 106. The iGUS activity persisted for approximately three days. Therefore, we assumed that E. coli present in fecal contaminants, in which GUS substrates are present, could be distinguished from those surviving in the natural environment for three days or longer by measuring iGUS activity. River water samples were collected upstream and downstream of the discharge outlets of municipal wastewater treatment plants and a combined sewer outlet. The iGUS activities were <0.24 mMFU/mL for the upstream samples and >0.21 mMFU/mL for the downstream samples. Interestingly, E. coli concentrations were not necessarily associated with fecal pollution. This indicates that by setting a threshold for iGUS activity, our method can be used as a simple and rapid method for detecting fecal pollution in urban rivers. Because the limit of detection for our method is 20 CFU/mL, our method is applicable to detecting high fecal pollution in a small river.

Analysis of Unamplified 16S rRNA of Ammonia-Oxidizing Bacteria in Activated Sludge by Spectrophotometry Using Gold Nanoprobes
Meri Nakajima, Reiko Hirano, Yuki Nakaya, Hisashi Satoh
ACS ES&T Water, American Chemical Society (ACS), 23 Aug. 2023
Scientific journal

Tracing morphological characteristics of activated sludge flocs by using a digital microscope and their effects on sludge dewatering and settling
Yuki Nakaya, Jinming Jia, Hisashi Satoh
Environmental Technology, 45, 20, 1, 11, Informa UK Limited, 04 Aug. 2023, [International Magazine]
English, Scientific journal, ABSTRACTIn wastewater treatment by the activated sludge (AS) process, settleability and dewaterability of AS are key issues that are directly related to the treated water quality and sludge treatment costs. Several studies investigated the relationship between the shape of AS flocs and their settling/dewatering property. To quantify the floc morphology, it is imperative to attach a camera to a microscope or move the stage manually. Hence, labour and equipment costs may increase. In this study, by combining a digital microscope and an automatic stage, more than 100 magnified floc images were rapidly obtained from one AS sample dropped on a slide glass, and shape parameters were collectively calculated using an analysis software. During 1-year monitoring of four wastewater treatment plants in Sapporo City (Hokkaido, Japan), the morphological parameters and extracellular polymeric substances (EPS) quantity/quality of AS were analyzed on the basis of their correlation to the time to filtration (TTF) and sludge volume index (SVI), which are indicators for describing the dewatering and settling properties of AS, respectively. In one plant, larger, denser, and smoother flocs tended to contain less EPS and exhibited better sludge dewaterability. In another plant, larger, denser, and smoother flocs were considered to contribute to better settlement. Especially, an equivalent high-density floc diameter and the ratio of mixed liquor suspended solids (MLSS) concentration to the total floc area were commonly suggested to explain AS dewaterability and settleability.

Towards an understanding of Candidate phyla radiation (CPR)/Candidatus Patescibacteria from perspectives of wastewater treatment systems
Kuroda Kyohei, Narihiro Takashi, Fujii Naoki, Nakajima Meri, Kagemasa Shuka, Nakai Ryosuke, Satoh Hisashi, Kubota Kengo, Kindaichi Tomonori
Japanese Society of Microbial Ecology, 38, 1, 2, 13, Japanese Society of Microbial Ecology, 01 Mar. 2023
Japanese

Highly sensitive and homogeneous detection of unamplified RNA based on the light scattering properties of gold nanoparticle probes
Hisashi Satoh, Meri Nakajima, Reiko Hirano, Yuki Nakaya
Biosensors and Bioelectronics: X, 12, 100249, 100249, Elsevier BV, Dec. 2022
Scientific journal, 40558276

Elucidation of the biodegradation pathways of bis(2-hydroxyethyl) terephthalate and dimethyl terephthalate under anaerobic conditions revealed by enrichment culture and microbiome analysis
Kyohei Kuroda, Takashi Narihiro, Yuki Nakaya, Taro Q.P. Noguchi, Ryota Maeda, Masaru K. Nobu, Yuki Ohnishi, Yasuhiro Kumaki, Tomoyasu Aizawa, Hisashi Satoh
Chemical Engineering Journal, 450, 1, 137916, 137916, Nov. 2022, [Peer-reviewed]
English, Scientific journal, 40558276

Separate determination of arsenite and arsenate in groundwater samples by simple analytical methods
Koji Matsunaga, Yuki Nakaya, Hisashi Satoh
Water Supply, 22, 10, 7635, 7642, IWA Publishing, 17 Sep. 2022, [Peer-reviewed]
English, Scientific journal, Abstract

We investigated the interaction of As(III) with As(III)-binding DNA aptamers (Ars-3). The binding of Ars-3 to As(III) was investigated using an Ars-3 sequence and a few control DNA sequences. As a result, we conclude that Ars-3 cannot bind As(III) and As(III) can adsorb onto gold nanoparticles with selectivity. However, the As(III) concentration could be determined by a simple As(III) analysis method using Ars-3, and we measured the As(III) and As(V) concentrations separately in As(III)/As(V) mixed samples in ultrapure water and groundwater using a simple analytical method for As(III) and As(V), respectively. As(III) in the sample was measured by a simple As(III) analytical method using DNA aptamers and gold nanoparticles, and As(V) in the sample was measured by a simple As(V) analysis method using cerium oxide nanoparticles and fluorescence-labeled DNA. We can measure As(III) and As(V) concentrations in ultrapure water and groundwater in the simple analytical method for As(III) and As(V), separately., 40558276

Screening Antibiotic-Resistant Escherichia coli in Wastewater and River Water Using a Novel Simple Phenotypic Antibiotic-Susceptibility Testing Method
Hisashi Satoh, Natsumi Nagahashi, Kai Kikuchi, Reiko Hirano
ACS EST Water, 2, 8, 1301, 1308, American Chemical Society (ACS), 27 Jun. 2022, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, 40558276

Development of the simple analytical method for determination of arsenate(V) ion using fluorescence-labeled DNA and cerium oxide nanoparticles
Koji Matsunaga, Hisashi Satoh, Reiko Hirano
Water Supply, 22, 5, 5524, 5534, IWA Publishing, 24 Mar. 2022, [Peer-reviewed]
English, Scientific journal, Abstract

Arsenic (As) contamination in groundwater presents a major health and environmental concern. As is found in two oxidation states and most chemical tests for inorganic arsenic are focused on As(III), and few have been developed for As(V). We developed the simple analytical method for determining As(V) concentrations in groundwater using CeO2NPs and fluorescein (FAM)-labeled DNA. Prior to sample measurements, we investigated the key operational parameters that affect the sensing performance. The optimal CeO2NPs final concentration, FAM-labeled DNA final concentration, the sequence and length of FAM-labeled DNA, and incubation time were 15 μg/mL, 400 nM, 6-mer poly-cytosine sequence, and 6 min, respectively. After optimizing the parameters, the total analysis time was about 20 min and the limit of detection was 0.61 μM. This method has a high selectivity against the same concentrations of Cu(II), Cd(II), Hg(II) and Pb(II). Pretreatment by cation extraction to remove interfering ions was beneficial for determination of As(V) concentrations in groundwater containing a variety of metal cations at high concentration. We could determine As(V) concentration in groundwater. Modification of the reactions of the method is necessary. This study provides the first step in the development of a simple method for on-site As(V) analysis., 40558276

Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
Hisashi Satoh, Yutaka Katayose, Reiko Hirano
Water Science and Technology, 83, 6, 1399, 1406, IWA Publishing, 15 Mar. 2021, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal,
Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the β-d-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at &gt;100 most probable numbers 100 mL−1 with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW., 13465201

Bubble drag in electrolytically generated microbubble swarms with bubble-vortex interactions
Yasunori Watanabe, Haruhi Oyaizu, Hisashi Satoh, Yasuo Niida
International Journal of Multiphase Flow, 136, 103541, 103541, Mar. 2021, [Peer-reviewed]
English, Scientific journal

Deep learning-based morphology classification of activated sludge flocs in wastewater treatment plants
Hisashi Satoh, Yukari Kashimoto, Naoki Takahashi, Takashi Tsujimura
Environmental Science: Water Research & Technology, Royal Society of Chemistry (RSC), 2021, [Lead author, Corresponding author]
Scientific journal,

A deep learning-based two-label classifier 1 recognized a 20% morphological change in the activated flocs. Classifier-2 quantitatively recognized an abundance of filamentous bacteria in activated flocs.

Simple assay for colorimetric quantification of unamplified bacterial 16S rRNA in activated sludge using gold nanoprobes
Meri Nakajima, Reiko Hirano, Satoshi Okabe, Hisashi Satoh
Chemosphere, 263, 128331, 128331, Elsevier BV, Jan. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Domestic and industrial wastewater treatment systems are vital in the protection of natural ecosystems and human health. Identification of microbial communities in the systems is essential to stable treatment performance. However, the current tools of microbial community analysis are labor intensive and time consuming, and require expensive equipment. Therefore, we developed a simple assay for colorimetric quantification of bacterial 16S rRNA extracted from environmental samples. The assay is based on RNA extraction with commercial kits, mixing the unamplified RNA sample with Au-nanoprobes and NaCl, and analyzing the absorbance spectra. Our experimental results confirmed that the assay format was valid. By analyzing the synthesized DNA, we optimized the operational parameters affecting the assay. We achieved adequate capture DNA density by setting the capture DNA probe concentration at 10 μM during the functionalization step. The required incubation time after NaCl addition was 30 min. The binding site of the target had negligible effect on DNA detection. Under the optimized condition, a calibration curve was created using 16S rRNA extracted from activated sludge. The curve was linear above 5.0 × 107 copies/μL of bacterial 16S rRNA concentration, and the limit of detection was 1.17 × 108 copies/μL. Using the calibration curve, the bacterial 16S rRNA concentration in activated sludge samples could be quantified with deviations between 48% and 208% against those determined by RT-qPCR. The findings of our study introduce an innovative tool for the quantification of 16S rRNA concentration as the activity of key bacteria in wastewater treatment processes, achieving stable treatment performance.

Development of a paper-based analytical chip for detection of bacterial 16S rRNA in wastewater samples
Meri Nakajima, Akihiko Ishida, Manabu Tokeshi, Hisashi Satoh
Bunseki kagaku, 69, 12, 715, 722, Dec. 2020, [Peer-reviewed], [Corresponding author]
Japanese, Scientific journal

Synthesis of a Fluorescent Solvatochromic Resin Using Suzuki–Miyaura Cross-Coupling and Its Optical Waveguide Spectra to Measure the Solvent Polarity on the Surface
Yu Otsuka, Guanglei Li, Hiromi Takahashi, Hisashi Satoh, Koji Yamada
Materials, 13, 20, 4483, 4483, MDPI AG, 10 Oct. 2020, [Peer-reviewed]
English, Scientific journal, We have established a novel analytical method for solvent polarity on resin surface by combining the synthesis of fluorescent solvatochromic resin with optical waveguide spectrometry. The fluorescent solvatochromic resin was obtained via Suzuki–Miyaura cross-coupling between 4-iodobenzoic acid immobilized on Wang resin and 5-[4-(N,N-dihexylamino)phenyl]-2-thienylboronic acid N-methyl-iminodiacetic acid (MIDA) ester. The optical waveguide spectrometry studies on the resin showed a strong fluorescent solvatochromism in various organic solvents., 11819065

Redox stratification within cryoconite granules influences the nitrogen cycle on glaciers
Takahiro Segawa, Nozomu Takeuchi, Hiroshi Mori, Rathnayake M L D Rathnayake, Zhongqin Li, Ayumi Akiyoshi, Hisashi Satoh, Satoshi Ishii
FEMS Microbiology Ecology, Oxford University Press (OUP), 29 Sep. 2020, [Peer-reviewed]
Scientific journal,
Cryoconite granules are naturally occurring microbial structures on glacier surfaces worldwide. They play a key role in carbon and nitrogen cycling in glacier ecosystems and can accelerate the melting of snow and ice. However, detailed mechanism of nitrogen cycling in cryoconite granules remain unclear. Here, we demonstrate that redox stratification affects the spatial distribution of N cycling processes in cryoconite granules. Based on microsensor measurements for O2, NH4+, NO2–, and NO3–, we identified the presence of fine scale redox stratification within cryoconite granules. Cyanobacteria at the surface layer of the granules created oxic conditions, whereas the inner core of the granules was anoxic. Metatranscriptomic analyses indicated the active occurrences of nitrification in the inner core, whereas denitrification actively occurred both in the inner core and the surface layer of the granules. Cyanobacteria in the inner core of the granules were inactive, and likely dead and being degraded, providing carbon and nitrogen to support nitrifiers and denitrifiers. Quantities of nitrification genes/transcripts were greater in large cryoconite granules than small ones, most likely because nitrogen substrates were more abundantly present in inner core of large granules due to distinct redox stratification. Our results suggest that development of a granular structure of cryoconite granules can largely affect carbon and nitrogen cycling on glaciers.

Cell-density dependent anammox activity of Candidatus Brocadia sinica regulated by N-acyl homoserine lactone-mediated quorum sensing
Mamoru Oshiki, Haruna Hiraizumi, Hisashi Satoh, Satoshi Okabe
Microbes Environ., 35, 4, Aug. 2020, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The activity of anaerobic ammonia-oxidizing (anammox) bacteria is considered to depend on cell density; however, this has not yet been confirmed due to the fastidious nature of anammox bacteria (e.g., slow growth, oxygen sensitivity, and rigid aggregate formation). In the present study, the cell density-dependent occurrence of anammox activity (14-15N2 gas production rate) was investigated using planktonic enrichment cultures of Candidatus Brocadia sinica. This activity was detectable when the density of cells was higher than 107‍ ‍cells‍ ‍mL-1 and became stronger with increases in cell density. At the cell densities, the transcription of the BROSI_A1042 and BROSI_A3652 genes, which are potentially involved in the biosynthesis and reception of N-acyl homoserine lactone (AHL), was detectable in Brocadia sinica cells. The presence of AHL molecules in the MBR culture of B. sinica was confirmed by an AHL reporter assay and gas chromatography mass spectrometry analysis. The exogenous addition of the MBR culture extract and AHL molecules (a cocktail of C6, C8, C10, and C12-homoserine lactones) increased the specific 14-15N2 production rate of B. sinica. These results suggest that the specific anammox activity of B. sinica is regulated by AHL-mediated quorum sensing.

Simple and reliable enumeration of Escherichia coli concentrations in wastewater samples by measuring β-D-glucuronidase (GUS) activities via a microplate reader
Hisashi Satoh, Kai Kikuchi, Yutaka Katayose, Shu Tsuda, Reiko Hirano, Yuga Hirakata, Masaaki Kitajima, Satoshi Ishii, Mamoru Oshiki, Masashi Hatamoto, Masahiro Takahashi, Satoshi Okabe
Science of the Total Environment, 715, 01 May 2020, [Peer-reviewed], [Lead author, Last author, Corresponding author]
English, Scientific journal, 13465201

特定酵素蛍光基質を用いた下水中の大腸菌群の簡易迅速測定法の開発
佐藤 久
下水道協会誌, 56, 684, 110, 117, Oct. 2019, [Peer-reviewed]
Japanese, Scientific journal, 11819084

Development of a simple analytical method to determine arsenite using a DNA aptamer and gold nanoparticles
Matsunaga, Koji, Okuyama, Yu, Hirano, Reiko, Okabe, Satoshi, Takahashi, Masahiro, Satoh, Hisashi
CHEMOSPHERE, 224, 538, 543, Jun. 2019, [Peer-reviewed]
English, Scientific journal, 11819065

Digestion performance and contributions of organic and inorganic fouling in an anaerobic membrane bioreactor treating waste activated sludge
Hafuka, Akira, Mashiko, Riho, Odashima, Ryuto, Yamamura, Hiroshi, Satoh, Hisashi, Watanabe, Yoshimasa
BIORESOURCE TECHNOLOGY, 272, 63, 69, Jan. 2019, [Peer-reviewed]
English, Scientific journal

Experimental Evidence for in Situ Nitric Oxide Production in Anaerobic Ammonia-Oxidizing Bacterial Granules
Rathnayake, Rathnayake M. L. D., Oshiki, Mamoru, Ishii, Satoshi, Segawa, Takahiro, Satoh, Hisashi, Okabe, Satoshi
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 52, 10, 5744, 5752, May 2018, [Peer-reviewed]
English, Scientific journal

3-[Bis(pyridin-2-ylmethyl)amino]-5-(4-carboxyphenyl)-BODIPY as Ratiometric Fluorescent Sensor for Cu2+
Hafuka, Akira, Satoh, Hisashi, Yamada, Koji, Takahashi, Masahiro, Okabe, Satoshi
MATERIALS, 11, 5, May 2018, [Peer-reviewed]
English, Scientific journal

Development of novel polysulfone membranes with embedded zirconium sulfate-surfactant micelle mesostructure for phosphate recovery from water through membrane filtration
Kenji Furuya, Akira Hafuka, Miho Kuroiwa, Hisashi Satoh, Yoshimasa Watanabe, Hiroshi Yamamura
WATER RESEARCH, 124, 521, 526, Nov. 2017, [Peer-reviewed]
English, Scientific journal

Enhancement of organic matter degradation and methane gas production of anaerobic granular sludge by degasification of dissolved hydrogen gas
Hisashi Satoh, Wasala M. K. R. T. W. Bandara, Manabu Sasakawa, Yoshihito Nakahara, Masahiro Takahashi, Satoshi Okabe
BIORESOURCE TECHNOLOGY, 244, 768, 775, Nov. 2017, [Peer-reviewed]
English, Scientific journal

Genetic diversity of marine anaerobic ammonium-oxidizing bacteria as revealed by genomic and proteomic analyses of "Candidatus Scalindua japonica'
Mamoru Oshiki, Keisuke Mizuto, Zen-ichiro Kimura, Tomonori Kindaichi, Hisashi Satoh, Satoshi Okabe
ENVIRONMENTAL MICROBIOLOGY REPORTS, 9, 5, 550, 561, Oct. 2017, [Peer-reviewed]
English, Scientific journal

Determination of Cadmium in Brown Rice Samples by Fluorescence Spectroscopy Using a Fluoroionophore after Purification of Cadmium by Anion Exchange Resin
Akira Hafuka, Akiyoshi Takitani, Hiroko Suzuki, Takuya Iwabuchi, Masahiro Takahashi, Satoshi Okabe, Hisashi Satoh
SENSORS, 17, 10, Oct. 2017, [Peer-reviewed]
English, Scientific journal

Improvement of a Phosphate Ion-selective Microsensor Using Bis(dibromophenylstannyl)methane as a Carrier
Hisashi Satoh, Yuji Miyazaki, Shou Taniuchi, Mamoru Oshiki, Rathnayake M. L. D. Rathnayake, Masahiro Takahashi, Satoshi Okabe
ANALYTICAL SCIENCES, 33, 7, 825, 830, Jul. 2017, [Peer-reviewed]
English, Scientific journal

High spatial resolution analysis of the distribution of sulfate reduction and sulfide oxidation in hypoxic sediment in a eutrophic estuary
Rathnayake M. L. D. Rathnayake, Shogo Sugahara, Hideaki Maki, Gen Kanaya, Yasushi Seike, Hisashi Satoh
WATER SCIENCE AND TECHNOLOGY, 75, 2, 418, 426, Jan. 2017, [Peer-reviewed]
English, Scientific journal

Performance of anaerobic membrane bioreactor during digestion and thickening of aerobic membrane bioreactor excess sludge
Akira Hafuka, Kazuhisa Mimura, Qing Ding, Hiroshi Yamamura, Hisashi Satoh, Yoshimasa Watanabe
BIORESOURCE TECHNOLOGY, 218, 476, 479, Oct. 2016, [Peer-reviewed]
English, Scientific journal

Source identification of nitrous oxide emission pathways from a single-stage nitritation-anammox granular reactor
Muhammad Ali, Rathnayake M. L. D. Rathnayake, Lei Zhang, Satoshi Ishii, Tomonori Kindaichi, Hisashi Satoh, Sakae Toyoda, Naohiro Yoshida, Satoshi Okabe
WATER RESEARCH, 102, 147, 157, Oct. 2016, [Peer-reviewed]
English, Scientific journal

Hydroxylamine-dependent anaerobic ammonium oxidation (anammox) by "Candidatus Brocadia sinica"
Mamoru Oshiki, Muhammad Ali, Kaori Shinyako-Hata, Hisashi Satoh, Satoshi Okabe
ENVIRONMENTAL MICROBIOLOGY, 18, 9, 3133, 3143, Sep. 2016, [Peer-reviewed]
English, Scientific journal

Effects of organic matter in livestock manure digester liquid on microbial community structure and in situ activity of anammox granules
Tomonori Kindaichi, Takanori Awata, Yuichiro Mugimoto, Rathnayake M. L. D. Rathnayake, Shinsuke Kasahara, Hisashi Satoh
CHEMOSPHERE, 159, 300, 307, Sep. 2016, [Peer-reviewed]
English, Scientific journal

Ecology and physiology of anaerobic ammonium oxidizing bacteria
Mamoru Oshiki, Hisashi Satoh, Satoshi Okabe
ENVIRONMENTAL MICROBIOLOGY, 18, 9, 2784, 2796, Sep. 2016, [Peer-reviewed]
English

Draft genome sequence of an anaerobic ammonium-oxidizing bacterium, "Candidatus Brocadia sinica"
Mamoru Oshiki, Kaori Shinyako-Hata, Hisashi Satoh, Satoshi Okabe
Genome Announcements, 3, 2, American Society for Microbiology, 2016, [Peer-reviewed]
English, Scientific journal

Effects of dissolved oxygen and pH on nitrous oxide production rates in autotrophic partial nitrification granules
Rathnayake M. L. D. Rathnayake, Mamoru Oshiki, Satoshi Ishii, Takahiro Segawa, Hisashi Satoh, Satoshi Okabe
BIORESOURCE TECHNOLOGY, 197, 15, 22, Dec. 2015, [Peer-reviewed]
English, Scientific journal

Interactions of dissolved humic substances with oppositely charged fluorescent dyes for tracer techniques
Akira Hafuka, Qing Ding, Hiroshi Yamamura, Koji Yamada, Hisashi Satoh
WATER RESEARCH, 85, 193, 198, Nov. 2015, [Peer-reviewed]
English, Scientific journal

Rapid and successful start-up of anammox process by immobilizing the minimal quantity of biomass in PVA-SA gel beads
Muhammad Ali, Mamoru Oshiki, Lashitha Rathnayake, Satoshi Ishii, Hisashi Satoh, Satoshi Okabe
WATER RESEARCH, 79, 147, 157, Aug. 2015, [Peer-reviewed]
English, Scientific journal

Physiological characterization of anaerobic ammonium oxidizing bacterium "CandidatusJettenia caeni'
Muhammad Ali, Mamoru Oshiki, Takanori Awata, Kazuo Isobe, Zenichiro Kimura, Hiroaki Yoshikawa, Daisuke Hira, Tomonori Kindaichi, Hisashi Satoh, Takao Fujii, Satoshi Okabe
ENVIRONMENTAL MICROBIOLOGY, 17, 6, 2172, 2189, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Draft Genome Sequence of an Anaerobic Ammonium-Oxidizing Bacterium, "Candidatus Brocadia sinica"
Oshiki, Mamoru, Shinyako-Hata, Kaori, Satoh, Hisashi, Okabe, Satoshi
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 3, 2, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Substituent Effects at the 5-Position of 3-[Bis(pyridine-2-ylmethyl)amino]-BODIPY Cation Sensor Used for Ratiometric Quantification of Cu2+
Akira Hafuka, Ryosuke Kando, Kohei Ohya, Koji Yamada, Satoshi Okabe, Hisashi Satoh
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 88, 3, 447, 454, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Efficient Nitrogen Removal by Anaerobic Ammonium Oxidation (anammox) Process
押木 守, 佐藤 久, 岡部 聡
Journal of environmental biotechnology, 14, 1, 21, 29, 環境バイオテクノロジー学会, Oct. 2014, [Peer-reviewed]
Japanese

Identification of key nitrous oxide production pathways in aerobic partial nitrifying granules
Satoshi Ishii, Yanjun Song, Lashitha Rathnayake, Azzaya Tumendelger, Hisashi Satoh, Sakae Toyoda, Naohiro Yoshida, Satoshi Okabe
ENVIRONMENTAL MICROBIOLOGY, 16, 10, 3168, 3180, Oct. 2014, [Peer-reviewed]
English, Scientific journal

Application of fluorescence spectroscopy using a novel fluoroionophore for quantification of zinc in urban runoff
Akira Hafuka, Hiroaki Yoshikawa, Koji Yamada, Tsuyoshi Kato, Masahiro Takahashi, Satoshi Okabe, Hisashi Satoh
WATER RESEARCH, 54, 12, 20, May 2014, [Peer-reviewed]
English, Scientific journal

Application of fluorescence spectroscopy using a novel fluoroionophore for quantification of zinc in urban runoff
Akira Hafuka, Hiroaki Yoshikawa, Koji Yamada, Tsuyoshi Kato, Masahiro Takahashi, Satoshi Okabe, Hisashi Satoh
Water Research, 54, 12, 20, Elsevier {BV}, 01 May 2014, [Peer-reviewed]
English, Scientific journal

Control of algal production in a high rate algal pond: investigation through batch and continuous experiments
H. Derabe Maobe, M. Onodera, M. Takahashi, H. Satoh, T. Fukazawa
WATER SCIENCE AND TECHNOLOGY, 69, 12, 2519, 2525, 2014, [Peer-reviewed]
English, Scientific journal

Source identification of nitrous oxide on autotrophic partial nitrification in a granular sludge reactor
R. M. L. D. Rathnayake, Y. Song, A. Tumendelger, M. Oshiki, S. Ishii, H. Satoh, S. Toyoda, N. Yoshida, S. Okabe
WATER RESEARCH, 47, 19, 7078, 7086, Dec. 2013, [Peer-reviewed]
English, Scientific journal

Cultivation of Planktonic Anaerobic Ammonium Oxidation (Anammox) Bacteria Using Membrane Bioreactor
Oshiki, Mamoru, Awata, Takanori, Kindaichi, Tomonori, Satoh, Hisashi, Okabe, Satoshi
MICROBES AND ENVIRONMENTS, 28, 4, 436, 443, Dec. 2013, [Peer-reviewed]
English, Scientific journal

Determination of Zn²⁺ in industrial wastewater by fluorescence spectroscopy with fluoroionophore
HAFUKA Akira, YOSHIKAWA Hiroaki, OHYA Kohei, YAMADA Koji, TAKAHASHI Masahiro, OKABE Satoshi, SATOH Hisashi
Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research), 69, 7, III_275, III_280, 公益社団法人 土木学会, Nov. 2013, [Peer-reviewed]
Japanese, We applied a novel fluorescent indicator (i.e., fluoroionophore) for industrial wastewater to determine Zn²⁺ by using fluorescence spectroscopy. The fluoroionophore showed red-shifted fluorescence upon Zn²⁺ binding. The indicator exhibited ratiometric fluorescence spectral change with increase of Zn²⁺ concentrations. Zn²⁺ was successfully determined in real industrial wastewater matrix by using the fluoroionophore. In this research, we could demonstrate the potential application of fluorescence spectroscopy with the fluoroionophore to determine heavy metal ions in real wastewater.

Nitrate-dependent ferrous iron oxidation by anaerobic ammonium oxidation (anammox) bacteria
M. Oshiki, S. Ishii, K. Yoshida, N. Fujii, M. Ishiguro, Hisashi Satoh, S. Okabe
Applied and Environmental Microbiology, 79, 13, 4087, 4093, Jul. 2013, [Peer-reviewed]
English, Scientific journal

High efficiency removal of phosphate from water by zirconium sulfate-surfactant micelle mesostructure immobilized on polymer matrix
Pitakteeratham, Niti, Hafuka, Akira, Satoh, Hisashi, Watanabe, Yoshimasa
WATER RESEARCH, 47, 11, 3583, 3590, Jul. 2013, [Peer-reviewed]
English, Scientific journal

Development and characterization of the partial nitrification aerobic granules in a sequencing batch airlift reactor
Yanjun Song, Satoshi Ishii, Lashitha Rathnayake, Tsukasa Ito, Hisashi Satoh, Satoshi Okabe
BIORESOURCE TECHNOLOGY, 139, 285, 291, Jul. 2013, [Peer-reviewed]
English, Scientific journal

Spatial and Temporal Oxygen Dynamics in Macrofaunal Burrows in Sediments: A Review of Analytical Tools and Observational Evidence
Satoh, Hisashi, Okabe, Satoshi
MICROBES AND ENVIRONMENTS, 28, 2, 166, 179, Jun. 2013, [Peer-reviewed]
English

Introduction of a Degassing Membrane Technology into Anaerobic Wastewater Treatment
W. M. K. R. T. W. Bandara, M. Ikeda, H. Satoh, M. Sasakawa, Y. Nakahara, M. Takahashi, S. Okabe
WATER ENVIRONMENT RESEARCH, 85, 5, 387, 390, May 2013, [Peer-reviewed]
English

Explicit temperature-based model for anaerobic digestion: Application in domestic wastewater treatment in a UASB reactor
A. Donoso-Bravo, W. M K R T W Bandara, H. Satoh, G. Ruiz-Filippi
Bioresource Technology, 133, 437, 442, Elsevier Ltd, 2013, [Peer-reviewed]
English, Scientific journal

BODIPY-Based Ratiometric Fluoroionophores with Bidirectional Spectral Shifts for the Selective Recognition of Heavy Metal Ions
Akira Hafuka, Hiroki Taniyama, Sang-Hyun Son, Koji Yamada, Masahiro Takahashi, Satoshi Okabe, Hisashi Satoh
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 86, 1, 37, 44, Jan. 2013, [Peer-reviewed]
English, Scientific journal

Anaerobic treatment of municipal wastewater at ambient temperature: Analysis of archaeal community structure and recovery of dissolved methane
Wasala M. K. R. T. W. Bandara, Tomonori Kindaichi, Hisashi Satoh, Manabu Sasakawa, Yoshihito Nakahara, Masahiro Takahashi, Satoshi Okabe
WATER RESEARCH, 46, 17, 5756, 5764, Nov. 2012, [Peer-reviewed]
English, Scientific journal

Community Structure and In Situ Activity of Nitrifying Bacteria in Phragmites Root-Associated Biofilms
Satoshi Okabe, Yoshiyuki Nakamura, Hisashi Satoh
MICROBES AND ENVIRONMENTS, 27, 3, 242, 249, Sep. 2012, [Peer-reviewed]
English, Scientific journal

Revealing microbial community structures in large- and small-scale activated sludge systems by barcoded pyrosequencing of 16S rRNA gene
Purnika Damindi Ranasinghe, Hiroyasu Satoh, Mamoru Oshiki, Kenshiro Oshima, Wataru Suda, Masahira Hattori, Takashi Mino
WATER SCIENCE AND TECHNOLOGY, 66, 10, 2155, 2161, 2012, [Peer-reviewed]
English, Scientific journal

Characterization of microbial community structures and their activities in single anaerobic granules by beta imaging, microsensors and fluorescence in situ hybridization
H. Satoh, I. Tsushima, Y. Miura, T. Ito, S. Okabe
WATER SCIENCE AND TECHNOLOGY, 65, 12, 2125, 2131, 2012, [Peer-reviewed]
English, Scientific journal

N2O emission from a partial nitrification-anammox process and identification of a key biological process of N2O emission from anammox granules
Satoshi Okabe, Mamoru Oshiki, Yoshitaka Takahashi, Hisashi Satoh
WATER RESEARCH, 45, 19, 6461, 6470, Dec. 2011, [Peer-reviewed]
English, Scientific journal

Development of long-term stable partial nitrification and subsequent anammox process
Satoshi Okabe, Mamoru Oshiki, Yoshitaka Takahashi, Flisashi Satoh
BIORESOURCE TECHNOLOGY, 102, 13, 6801, 6807, Jul. 2011, [Peer-reviewed]
English, Scientific journal

Physiological characteristics of the anaerobic ammonium-oxidizing bacterium 'Candidatus Brocadia sinica'
Mamoru Oshiki, Masaki Shimokawa, Naoki Fujii, Hisashi Satoh, Satoshi Okabe
MICROBIOLOGY-SGM, 157, 1706, 1713, Jun. 2011, [Peer-reviewed]
English, Scientific journal

Removal of residual dissolved methane gas in an upflow anaerobic sludge blanket reactor treating low-strength wastewater at low temperature with degassing membrane
Wasala M. K. R. T. W. Bandara, Hisashi Satoh, Manabu Sasakawa, Yoshihito Nakahara, Masahiro Takahashi, Satoshi Okabe
WATER RESEARCH, 45, 11, 3533, 3540, May 2011, [Peer-reviewed]
English, Scientific journal

Effect of feeding regimens on polyhydroxybutyrate production from food wastes by Cupriavidus necator
Akira Hafuka, Kenji Sakaida, Hisashi Satoh, Masahiro Takahashi, Yoshimasa Watanabe, Satoshi Okabe
BIORESOURCE TECHNOLOGY, 102, 3, 3551, 3553, Feb. 2011, [Peer-reviewed]
English, Scientific journal

Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A
M. Oshiki, H. Satoh, T. Mino
WATER SCIENCE AND TECHNOLOGY, 64, 3, 747, 753, 2011, [Peer-reviewed]
English, Scientific journal

A POLYPHASIC APPROACH TO STUDY ECOPHYSIOLOGY OF COMPLEX MULTISPECIES NITRIFYING BIOFILMS
Satoshi Okabe, Hisashi Satoh, Tomonori Kindaichi
METHODS IN ENZYMOLOGY, VOL 46: RESEARCH ON NITRIFICATION AND RELATED PROCESSES, PT B, 496, 163, 184, 2011, [Peer-reviewed]
English, In book

Nitrogen removal performance and microbial community analysis of an anaerobic up-flow granular bed anammox reactor
Sunja Cho, Yoshitaka Takahashi, Naoki Fujii, Yohei Yamada, Hisashi Satoh, Satoshi Okabe
CHEMOSPHERE, 78, 9, 1129, 1135, Feb. 2010, [Peer-reviewed]
English, Scientific journal

Microbial community structures and in situ sulfate-reducing and sulfur-oxidizing activities in biofilms developed on mortar specimens in a corroded sewer system
Hisashi Satoh, Mitsunori Odagiri, Tsukasa Ito, Satoshi Okabe
WATER RESEARCH, 43, 18, 4729, 4739, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Layered structure of bacterial and archaeal communities and their in situ activities in anaerobic granules
Hisashi Satoh, Yuki Miura, Ikuo Tsushima, Satoshi Okabe
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73, 22, 7300, 7307, Nov. 2007, [Peer-reviewed]
English, Scientific journal

In situ activity and spatial organization of anaerobic ammonium-oxidizing (anammox) bacteria in biofilms
Tomonori Kindaichi, Ikuo Tsushima, Yuji Ogasawara, Masaki Shimokawa, Noriatsu Ozaki, Hisashi Satoh, Satoshi Okabe
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73, 15, 4931, 4939, Aug. 2007, [Peer-reviewed]
English, Scientific journal

Development of high-rate anaerobic ammonium-oxidizing (anammox) biofilm reactors
Ikuo Tsushima, Yuji Ogasawara, Tomonori Kindaichi, Hisashi Satoh, Satoshi Okabe
WATER RESEARCH, 41, 8, 1623, 1634, Apr. 2007, [Peer-reviewed]
English, Scientific journal

Succession of sulfur-oxidizing bacteria in the microbial community on corroding concrete in sewer systems
Satoshi Okabe, Mitsunori Odagiri, Tsukasa Ito, Hisashi Satoh
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73, 3, 971, 980, Feb. 2007, [Peer-reviewed]
English, Scientific journal

Influences of infaunal burrows on the community structure and activity of ammonia-oxidizing bacteria in intertidal sediments
Hisashi Satoh, Yoshiyuki Nakamura, Satoshi Okabe
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73, 4, 1341, 1348, Feb. 2007, [Peer-reviewed]
English, Scientific journal

Response of NIPAAm-Ch gel to temperature changes and its effectiveness on nitrification as medium for immobilization
Linjiang Yuan, Tetsuya Kusuda, Hisashi Satoh
JOURNAL OF APPLIED POLYMER SCIENCE, 103, 2, 681, 686, Jan. 2007, [Peer-reviewed]
English, Scientific journal

Population dynamics and in situ kinetics of nitrifying bacteria in autotrophic nitrifying biofilms as determined by real-time quantitative PCR
Tomonori Kindaichi, Yoshiko Kawano, Tsukasa Ito, Hisashi Satoh, Satoshi Okabe
BIOTECHNOLOGY AND BIOENGINEERING, 94, 6, 1111, 1121, Aug. 2006, [Peer-reviewed]
English, Scientific journal

Community structures and activities of nitrifying and denitrifying bacteria in industrial wastewater-treating biofilms
H Satoh, T Yamakawa, T Kindaichi, T Ito, S Okabe
BIOTECHNOLOGY AND BIOENGINEERING, 94, 4, 762, 772, Jul. 2006, [Peer-reviewed]
English, Scientific journal

Community structure, abundance, and in situ activity of nitrifying bacteria in river sediments as determined by the combined use of molecular techniques and microelectrodes
Y Nakamura, H Satoh, T Kindaichi, S Okabe
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 40, 5, 1532, 1539, Mar. 2006, [Peer-reviewed]
English, Scientific journal

Use of microelectrodes to investigate the effects of 2-chlorophenol on microbial activities in biofilms
H Satoh, Y Sasaki, Y Nakamura, S Okabe, T Suzuki
BIOTECHNOLOGY AND BIOENGINEERING, 91, 2, 133, 138, Jul. 2005, [Peer-reviewed]
English, Scientific journal

Succession of internal sulfur cycles and sulfur-oxidizing bacterial communities in microaerophilic wastewater biofilms
S Okabe, T Ito, K Sugita, H Satoh
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71, 5, 2520, 2529, May 2005, [Peer-reviewed]
English, Scientific journal

Photosynthesis in sediments determined at high spatial resolution by the use of microelectrodes
Y Nakamura, H Satoh, S Okabe, Y Watanabe
WATER RESEARCH, 38, 9, 2440, 2448, May 2004, [Peer-reviewed]
English, Scientific journal

Macroscale and microscale analyses of nitrification and denitrification in biofilms attached on membrane aerated biofilm reactors
H Satoh, H Ono, B Rulin, J Kamo, S Okabe, KI Fukushi
WATER RESEARCH, 38, 6, 1633, 1641, Mar. 2004, [Peer-reviewed]
English, Scientific journal

Analysis of microbial community structure and in situ activity of nitrifying biofilms
Okabe S, Satoh H, Ito T, Watanabe Y
Journal of Water and Environment Technology, 2, 2, 65, 74, Japan Society on Water Environment, 2004
English, Wastewater biofilms are very complex multispecies biofilms, displaying considerable heterogeneity with respect to both the microorganisms present and their physicochemical microenvironments. To understand the eco-physiology of individual microorganisms in the biofilm, techniques and tools with a high spatial and temporal resolution are required for direct detection of the spatial distributions of microbial species and their activities in minimally disturbed their natural habitats (e.g., biofilms). In this paper, we will, therefore, address the great potential of the combined use of the current FISH technique and microelectrodes to study the microbial ecology of complex microbial communities such as biofilms. The combination of these two techniques will provide reliable and direct information about relationships between in situ microbial activity and the occurrence of specific microorganisms in biofilms. As an example of the combined study, we will illustrate the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria on fine scale in autotrophic nitrifying biofilms by applying the full-cycle of 16S rRNA approach followed by fluorescence in situ hybridization (FISH), which is linked to their in situ activity distributions at a similar resolution determined by use of microelectrodes. The combination of these techniques allows relating in situ microbial activity directly to occurrence of nitrifying bacteria population.

Effects of hydroxylamine on microbial community structure and function of autotrophic nitrifying biofilms determined by in situ hybridization and the use of microelectrodes
T Kindaichi, S Okabe, H Satoh, Y Watanabe
WATER SCIENCE AND TECHNOLOGY, 49, 11-12, 61, 68, 2004, [Peer-reviewed]
English, Scientific journal

Analysis of size distribution and areal cell density of ammonia-oxidizing bacterial microcolonies in relation to substrate microprofiles in biofilms
S Okabe, T Kindaichi, T Ito, H Satoh
BIOTECHNOLOGY AND BIOENGINEERING, 85, 1, 86, 95, Jan. 2004, [Peer-reviewed]
English, Scientific journal

Sulfate-reducing bacterial community structure and their contribution to carbon mineralization in a wastewater biofilm growing under microaerophilic conditions
S Okabe, T Ito, H Satoh
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 63, 3, 322, 334, Dec. 2003, [Peer-reviewed]
English, Scientific journal

Effect of oxygen concentration on nitrification and denitrification in single activated sludge flocs
H Satoh, Y Nakamura, H Ono, S Okabe
BIOTECHNOLOGY AND BIOENGINEERING, 83, 5, 604, 607, Sep. 2003, [Peer-reviewed]
English, Scientific journal

Evaluation of the impact of bioaugmentation and biostimulation by in situ hybridization and microelectrode
H Satoh, S Okabe, Y Yamaguchi, Y Watanabe
WATER RESEARCH, 37, 9, 2206, 2216, May 2003, [Peer-reviewed]
English, Scientific journal

バイオフィルム内における硫黄循環と関連微生物群集構造の解析　　　　　　　　　　　　　　　
岡部聡, 伊藤司, 佐藤久
月刊 海洋, 35, 54, 63, 2003

Effect of nitrite and nitrate on biogenic sulfide production in sewer biofilms determined by the use of microelectrodes
S Okabe, T Ito, H Satoh, Y Watanabe
WATER SCIENCE AND TECHNOLOGY, 47, 11, 281, 288, 2003, [Peer-reviewed]
English, Scientific journal

Successional development of sulfate-reducing bacterial populations and their activities in a wastewater biofilm growing under microaerophilic conditions
T Ito, S Okabe, H Satoh, Y Watanabe
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68, 3, 1392, 1402, Mar. 2002, [Peer-reviewed]
English, Scientific journal

In Situ Analysis of Oxygen Consumption in Activated Sludge as Determined by the Use of Microelectrodes
SATOH Hisashi, NAKAMURA Yoshiyuki, ONO Hideki
Japan journal of water pollution research, 25, 1, 27, 32, Japan Society on Water Environment, 10 Jan. 2002
Japanese, Microelectrode was used to investigate oxygen consumption mechanism in activated sludge. Oxygen fluxes and distributions of oxygen consumption activity were determined from the microprofiles. The spatial distributions of oxygen and nitrate consumption activity were heterogeneous. Under conditions in which oxygen concentration in the bulk liquid was less than 120μM, oxygen consumption activity was high in the outer zone and anoxic zone developed in the center part of the activated sludge. Oxygen consumption activity of the activated sludge was comparable to that of the domestic wastewater biofilm. Distribution of nitrate consumption activity showed that denitrification occurred in the anoxic zone of the activated sludge. Oxygen consumption activity increased as oxygen concentration in the bulk liquid increased, which resulted in increase in oxygen fluxes of the activated sludge. In contrast, when oxygen concentration in the bulk liquid was more than 120μM, oxygen fluxes were unchanged due to limitation of microbial density. Oxygen fluxes at more than 120μM of oxygen in the bulk liquid were lower than those at about 120μM of oxygen.

Structure and function of nitrifying biofilms as determined by molecular techniques and the use of microelectrodes
S Okabe, H Naitoh, H Satoh, Y Watanabe
WATER SCIENCE AND TECHNOLOGY, 46, 1-2, 233, 241, 2002, [Peer-reviewed]
English, Scientific journal

Analysis of microbial structure and function of nitrifying biofilms
S Okabe, H Satoh, Y Watanabe
MICROBIAL GROWTH IN BIOFILMS, PT B, 337, 213, 224, 2001, [Peer-reviewed]
English

Significance of substrate C/N ratio on structure and activity of nitrifying biofilms determined by in situ hybridization and the use of microelectrodes
H Satoh, S Okabe, N Norimatsu, Y Watanabe
WATER SCIENCE AND TECHNOLOGY, 41, 4-5, 317, 321, 2000, [Peer-reviewed]
English, Scientific journal

Analyses of spatial distributions of sulfate-reducing bacteria and their activity in aerobic wastewater biofilms
S Okabe, T Itoh, H Satoh, Y Watanabe
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 65, 11, 5107, 5116, Nov. 1999, [Peer-reviewed]
English, Scientific journal

In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes
S Okabe, H Satoh, Y Watanabe
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 65, 7, 3182, 3191, Jul. 1999, [Peer-reviewed]
English, Scientific journal

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique
S Okabe, H Satoh, T Itoh, Y Watanabe
WATER SCIENCE AND TECHNOLOGY, 39, 7, 41, 47, 1999, [Peer-reviewed]
English, Scientific journal

Sulfate reduction and sulfide oxidation in aerobic mixed population biofilms
S Okabe, T Matsuda, H Satoh, T Itoh, Y Watanabe
WATER SCIENCE AND TECHNOLOGY, 37, 4-5, 131, 138, 1998, [Peer-reviewed]
English, Scientific journal

Transport of Inert Fluorescent Microparticles in Multispecies Biofilms
OKABE Satoshi, YASUDA Takeo, SATOH Hisashi, WATANABE Yoshimasa
Doboku Gakkai Ronbunshuu G, 33, 103, 114, Japan Society of Civil Engineers, 1996
The dynamics of spatial distribution of particulate components in mixed population biofilms was investigated using lμm fluorescent microparticles as tracer. Shifts of the tracer bead spatial distribution in the biofilm were measured by sectioning the biofilm with a microslicer and were compared with model simulations to evaluate the growthadvection concept in the existing biofilm models. The tracer beads could traverse throughout a 360 μm thick biofilm within 23 minutes, could be attributed to advective transport via water channels and pores. The release of the entrapped beads were much slower than predicted by a one dimensional model due to spatial and temporal changes in cell density in the biofilm. This evidence implied that cell growth and advection was not balanced due to the presence of biofilm heterogeneity (e. g., pores and voids). Three dimensional observation by a confocal scanning laser microscope clearly indicated that the biofilm consisted of semicountiguous base film and relatively high thickness variation of loose surface film. The substratum was not uniformly covered by the biofilm and cell-free spaces and voids were observed near the substratum. This suggested that the pores, voids and cell-free spaces in the biofilm were firstly filled with growing biomass, thereafter displacement of the tracer beads took place once the cell density reached certain levels. Model assumptions of constant cell density and a continuum concept (flat biomass) are clearly over simplified and should be revised. It was concluded that microbial population dynamics in the biofilm can be determined by not only microbial growth kinetics and physiology, but also by the biofilm structure and growth pattern. One dimensional approach (modeling) is, therefore, adequate to predict biofilm accumulation and its performance, but inadequate to accurately describe the microbial population dynamics in the biofilm.

Characterization of Suspended Solids Settling Properties in Effluent from Sewage Treatment
信時圭, 渡部靖憲, 中屋佑紀, 佐藤久, 日本水環境学会年会講演集, 59th, 2025

最終沈殿池上澄水中の懸濁物質の沈降特性解析
信時圭, 渡部靖憲, 中屋佑紀, 佐藤久, 下水道研究発表会講演集, 62nd, 2025

Elucidation of aerobic p-toluic acid degradation mechanisms in a down-flow hanging sponge reactor by multiomics analysis
高井麻帆, 中屋佑紀, 佐藤久, 相沢智康, 大西裕季, 熊木康弘, 成廣隆, 黒田恭平, 日本水環境学会年会講演集, 58th, 2024

Microscopic and metatranscriptomic analyses revealed unique cross-domain symbiosis betweenCandidatusPatescibacteria/candidate phyla radiation (CPR) and methanogenic archaea in anaerobic ecosystems
Kyohei Kuroda, Meri Nakajima, Ryosuke Nakai, Yuga Hirakata, Shuka Kagemasa, Kengo Kubota, Taro Q.P. Noguchi, Kyosuke Yamamoto, Hisashi Satoh, Masaru K. Nobu, Takashi Narihiro, bioRxiv, 15, 3, e0310223, 25 Aug. 2023, [International Magazine]
To verify the parasitic lifestyle ofCandidatusPatescibacteria in the enrichment cultures derived from a methanogenic bioreactor, we applied multifaceted approaches combining cultivation, microscopy, metatranscriptomic, and protein structure prediction analyses. Cultivation experiments with the addition of exogenous methanogenic archaea with acetate, amino acids, and nucleoside monophosphates and 16S rRNA gene sequencing confirmed the increase in the relative abundance ofCa. Patescibacteria and methanogens. The predominantCa. Patescibacteria wereCa. Yanofskybacteria and 32-520 lineages (to which belongs to classCa. Paceibacteria) and positive linear relationships (r²≥ 0.70) between the relative abundance ofCa. Yanofskybacteria andMethanothrix, suggesting that the tendency of the growth rate is similar to that of the host. By fluorescencein situhybridization (FISH) observations, the FISH signals ofMethanothrixandMethanospirillumcells withCa. Yanofskybacteria and with 32-520 lineages, respectively, were significantly lower than those of the methanogens withoutCa. Patescibacteria, suggesting their parasitic interaction. The TEM and SEM observations also support parasitism in that the cell walls and plugs of these methanogens associated with submicron cells were often deformed. In particular, someMethanothrix-like filamentous cells were dented where the submicron cells were attached. Metatranscriptomic and protein structure prediction analyses identified highly expressed secreted genes from the genomes ofCa. Yanofskybacteria and 32-520, and these genes contain adhesion-related domains to the host cells. Considering the results through the combination of microscopic observations, gene expression, and computational protein modeling, we propose that the interactions betweenCa. Yanofskybacteria and 32-520 belonging to classCa. Paceibacteria and methanogenic archaea are parasitism., Cold Spring Harbor Laboratory, English

金ナノ粒子プローブを用いた簡易核酸分析法の反応機構の検証
廣井楓夏, 三浦篤志, 黒田恭平, 成廣隆, 中屋佑紀, 佐藤久, 日本分析化学会年会講演要旨集(Web), 72nd, 2023

Development of rapid quantification techniques for methanogenic archaea and parasitic ultramicrobacteria using PCR-free gold nanoparticle probes
半田久純, 中島芽梨, 中屋佑紀, 佐藤久, 黒田恭平, 成廣隆, 日本水環境学会年会講演集, 57th, 2023

Longitudinal Analysis of DOM and Biochemical Parameters of Rivers in Hokkaido Focusing on agriculture- and sewage-derived substances
中屋佑紀, 赤澤優弥, 小野寺岳史郎, 宮島龍太, 佐藤久, 北林里佳子, 田中佑奈, 相沢智康, 大西裕季, 熊木康裕, 黒田恭平, 成廣隆, 日本水環境学会年会講演集, 57th, 2023

Challenges in Establishing a Co-culture System of Candidatus Patescibacteria/CPR and Methanogenic Archaea
中島芽梨, 中島芽梨, 中井亮佑, 久保田健吾, 中屋佑紀, 佐藤久, 延優, 成廣隆, 黒田恭平, 日本水環境学会年会講演集, 57th, 2023

金ナノ粒子プローブを用いたメタン生成アーキアと寄生性超微小細菌の迅速定量
半田久純, 中島芽梨, 中屋佑紀, 佐藤久, 成廣隆, 黒田恭平, 下水道研究発表会講演集, 60th, 2023

Fate of Antibiotic Resistance Genes (ARGs) in Urban River
小野寺岳史郎, 岩崎隼, 赤澤優弥, 宮島龍太, 中屋佑紀, 佐藤久, 平野麗子, 黒田恭平, 成廣隆, 日本水環境学会年会講演集, 56th, 2022

金ナノ粒子を用いた硝化細菌の16S rRNAの比色分析—第57回下水道研究発表会講演集
中島 芽梨, 佐藤 久, 平野 麗子, 下水道研究発表会講演集, 57, 16, 18, 2020
日本下水道協会, Japanese

中空糸膜槽外型の嫌気性MBRによる下水汚泥の消化
益子理保, 羽深昭, 山村寛, 小田嶋龍飛, 佐藤久, 渡辺義公, 日本水環境学会年会講演集, 52nd, 271, 09 Mar. 2018
Japanese

下廃水中からのリン回収に向けたジルコニウム‐メソ構造体包含ポリスルフォン膜の開発
古屋謙治, 羽深昭, 黒岩美帆, 山村寛, 佐藤久, 渡辺義公, 日本水環境学会年会講演集, 52nd, 60, 09 Mar. 2018
Japanese

下水処理水中のβ-グルクロニダーゼ活性を利用した簡易迅速大腸菌定量法の開発
菊地凱, 片寄由貴, 石井聡, 北島正章, 岡部聡, 高橋正宏, 佐藤久, 日本水環境学会年会講演集, 52nd, 2018

DNAアプタマーを用いた簡易ノロウイルス検出法の開発
吉原光, 北島正章, 岡部聡, 高橋正宏, 佐藤久, 佐野大輔, 日本水環境学会年会講演集, 52nd, 2018

β-グルクロニダーゼを用いた新規簡易大腸菌数測定法の開発
菊地凱, 片寄由貴, 北島正章, 高橋正宏, 岡部聡, 佐藤久, 平野麗子, 下水道研究発表会講演集, 55th, 2018

嫌気性MBRを用いた下水汚泥のメタン発酵
羽深昭, 小田嶋龍飛, 益子理保, 山村寛, 佐藤久, 渡辺義公, 下水道研究発表会講演集, 54th, 221‐223, 03 Jul. 2017
Japanese

嫌気性MBRの連続運転による下水汚泥の濃縮と消化
羽深昭, 小田嶋龍飛, 山村寛, 三村和久, 伊東賢洋, 佐藤久, 渡辺義公, 日本水環境学会年会講演集, 51st, 67, 09 Mar. 2017
Japanese

ジルコニウム‐メソ多孔体を包含したリン回収多機能膜の開発
古屋謙治, 羽深昭, 黒岩美帆, 山村寛, 佐藤久, 渡辺義公, 日本水環境学会年会講演集, 51st, 232, 09 Mar. 2017
Japanese

大腸菌のβ-グルクロニダーゼ生産能の解析
菊地凱, 北島正章, 岡部聡, 高橋正宏, 佐藤久, 環境工学研究フォーラム講演集, 54th, 2017

DNAアプタマーを用いた簡易ノロウイルス検出法の開発
吉原光, 北島正章, 佐野大輔, 岡部聡, 高橋正宏, 佐藤久, 環境工学研究フォーラム講演集, 54th, 2017

DNAアプタマーを用いた簡易ノロウイルス検出法の開発
吉原光, 北島正章, 佐野大輔, 岡部聡, 高橋正宏, 佐藤久, 全国会議(水道研究発表会)講演集, 2017, 2017

鉄還元型嫌気性アンモニア酸化プロセスによる超省エネルギーかつ省コストな窒素除去技術の創出
押木守, 荒木信夫, 川上周司, 佐藤久, 環境助成研究成果 概要集 第35回・2014年度分:5件 第36回・2015年度分:28件 平成29年, 25‐26, 2017
Japanese

リン吸着能を有するジルコニウム担持分離膜の開発
古屋謙治, 羽深昭, 黒岩美帆, 佐藤久, 渡辺義公, 山村寛, 環境工学研究フォーラム講演集, 53rd, 15, 06 Dec. 2016
Japanese

嫌気性膜分離法(AnMBR)を用いた余剰汚泥の消化と濃縮に関する研究
小田嶋龍飛, 羽深昭, 佐藤久, 渡辺義公, 山村寛, 環境工学研究フォーラム講演集, 53rd, 18, 06 Dec. 2016
Japanese

嫌気性膜分離(AnMBR)を用いたMBR余剰汚泥の消化に関する研究
羽深昭, 山村寛, 佐藤久, 渡辺義公, 下水道研究発表会講演集, 53rd, 1130‐1132, 1132, 01 Jul. 2016
日本下水道協会, Japanese

嫌気性MBRを用いたMBR余剰汚泥の消化
羽深昭, 山村寛, 三村和久, 佐藤久, 渡辺義公, 日本水環境学会年会講演集, 50th, 181, 10 Mar. 2016
Japanese

河川水中における河床付着藻類が大腸菌の増殖に及ぼす影響—第53回下水道研究発表会講演集
上田 晃大, 上西 翔太, 高橋 正宏, 佐藤 久, 深澤 達矢, 下水道研究発表会講演集, 53, 515, 517, 2016
東京 : 日本下水道協会, Japanese

下水処理水中カリウムの回収に向けた好塩古細菌の動力学的解析 (第53回下水道研究発表会講演集)
楊 子然, 森 康弘, 中川 晴菜, 野村 惇喜, 高橋 正宏, 佐藤 久, 深澤 達矢, 下水道研究発表会講演集, 53, 959, 961, 2016
日本下水道協会, Japanese

カリウム回収の仕上げ工程としての塩化物混合物からの塩化カリウムの分離
中山翔太, 高橋正宏, 佐藤久, 深澤達矢, 中川晴菜, 第５２回下水道研究発表会講演集, 52, 449, 451, Jul. 2015
日本下水道協会, Japanese, Summary national conference

嫌気性MBRを用いたbMBR余剰汚泥からのリン溶出に関する研究
LU Tao, 羽深昭, 山村寛, 渡辺義公, 佐藤久, 下水道研究発表会講演集, 52nd, 197, 199, 01 Jul. 2015
日本下水道協会, Japanese

雑排水の農業利用のための高速酸化池におけるLASの除去
中山翔太, 高橋 正宏, 佐藤久, 深澤達矢, 第５１回下水道研究発表会講演集, 51, 304, 306, Jul. 2014
日本下水道協会, Japanese, Summary national conference

O21-02 Identification of key N_2O production pathways in aerobic partial nitrifying granules :
Ishii Satoshi, Song Yanjun, Rathnayake Lashitha, Tumendelger Azzaya, Satoh Hisashi, Sakae Toyoda, Yoshida Naohiro, Okabe Satoshi, 日本微生物生態学会講演要旨集, 2014, 94, 94, 2014
日本微生物生態学会, English

ゲノミクス,プロテオミクス解析から読み解くアンモニア酸化(anammox)細菌“Ca.Scalindua japonica”の生活環
押木守, 水戸佳祐, 木村善一郎, 金田一智規, 佐藤久, 岡部聡, 日本ゲノム微生物学会年会要旨集, 8th, 69, 2014
Japanese

2S-Cp04 Physiological and genomic insights into an anammox bacterium "Candidatus Brocadia sinica"
Oshiki Mamoru, Satoh Hisashi, Okabe Satoshi, 日本生物工学会大会講演要旨集, 65, 89, 89, 25 Aug. 2013
公益社団法人日本生物工学会, Japanese

変色型ボロンジピロメテン蛍光色素を用いた路面排水中Zn²⁺濃度の定量
羽深昭, 吉川弘晃, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 分析化学討論会講演要旨集, 73rd, 149, 04 May 2013
Japanese

網羅的重金属イオン分析に向けた新規変色型蛍光色素の開発
吉川弘晃, 羽深昭, 菅藤亮輔, 大屋光平, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 47th, 431, 11 Mar. 2013
Japanese

環境サンプル測定のためのリン酸マイクロセンサーの開発
宮崎悠爾, 谷内翔, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 47th, 321, 11 Mar. 2013
Japanese

新規変色型蛍光色素を用いた路面排水中亜鉛濃度の定量
菅藤亮輔, 羽深昭, 吉川弘晃, 大屋光平, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 47th, 432, 11 Mar. 2013
Japanese

重金属測定用蛍光色素の理論的分子設計による高感度化
大屋光平, 羽深昭, 菅藤亮輔, 吉川弘晃, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 47th, 692, 11 Mar. 2013
Japanese

リン酸マイクロセンサーのイオノフォアの検討
谷内翔, 宮崎悠爾, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 47th, 322, 11 Mar. 2013
Japanese

表面プラズモン共鳴を利用した病原微生物バイオセンサの開発
坂槙有紀恵, 山田健太, 佐野大輔, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 47th, 436, 11 Mar. 2013
Japanese

環境サンプル測定のためのリン酸マイクロセンサーの開発
宮崎悠爾, 谷内翔, 押木守, 佐藤久, 高橋正宏, 岡部聡, 土木学会北海道支部論文報告集(CD-ROM), 69, ROMBUNNO.G-02, Feb. 2013
Japanese

アフリカ-サヘル地域における高速酸化池法の再生水中固形物・栄養塩濃度の制御 (第50回下水道研究発表会講演集)
小野寺 正純, 高橋 正宏, 佐藤 久, 下水道研究発表会講演集, 50, 103, 105, 2013
日本下水道協会, Japanese

東京湾奥部における底質間隙水中の遊離の硫化水素について
牧秀明, 金谷弦, 中村泰男, 管原庄吾, 清家泰, 佐藤久, 日本海洋学会大会講演要旨集, 2013, 2013

環境サンプル測定のためのリン酸マイクロセンサーの開発
宮崎悠爾, 谷内翔, 押木守, 高橋正宏, 岡部聡, 佐藤久, 衛生工学シンポジウム論文集, 20th, 24, 04 Dec. 2012
Japanese

表面プラズモン共鳴を利用した病原微生物バイオセンサの開発
坂槙有紀恵, 山田健太, ピタックティーラタム ニティ, 石井聡, 佐野大輔, 高橋正宏, 岡部聡, 佐藤久, 環境工学研究フォーラム講演集, 49th, 28, 30, 28 Nov. 2012
Japanese

新規蛍光分子プローブを用いた環境水中重金属イオン分析
菅藤亮輔, 羽深昭, 吉川弘晃, 大屋光平, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 環境工学研究フォーラム講演集, 49th, 31, 33, 28 Nov. 2012
Japanese

環境サンプル測定のためのリン酸マイクロセンサーの開発
宮崎悠爾, 谷内翔, 押木守, 佐藤久, 高橋正宏, 岡部聡, 環境工学研究フォーラム講演集, 49th, 25, 27, 28 Nov. 2012
Japanese

PGa-04 Elucidating a metabolic pathway for the anaerobic ammonium oxidation of Candidatus "Brocadia sinica" by metagenomics analysis
Oshiki Mamoru, Shinyako Kaori, Satoh Hisashi, Okabe Satoshi, 日本微生物生態学会講演要旨集, 28, 160, 160, 19 Sep. 2012
日本微生物生態学会, English

表面プラズモン共鳴を利用した水中病原微生物検出バイオセンサの開発
佐藤久, 坂槙有紀恵, 山田健太, ピタックティーラタムニティ, 石井聡, 佐野大輔, 高橋正宏, 岡部聡, 日本水環境学会シンポジウム講演集, 15th, 233, 234, 10 Sep. 2012
Japanese

嫌気性アンモニア酸化(アナモックス)細菌のゲノム解析が明らかにする生理学的特性
押木守, 佐藤久, 岡部聡, 新家子香織, 日本水環境学会シンポジウム講演集, 15th, 157, 10 Sep. 2012
Japanese

トリベンジルチンクロライドを用いたリン酸マイクロセンサーの開発
谷内翔, 宮崎悠爾, 高橋正宏, 岡部聡, 佐藤久, 日本分析化学会年会講演要旨集, 61st, 324, 05 Sep. 2012
Japanese

新規変色型蛍光色素を用いた路面排水中亜鉛濃度の定量
吉川弘晃, 菅藤亮輔, 羽深昭, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本分析化学会年会講演要旨集, 61st, 315, 05 Sep. 2012
Japanese

表面プラズモン共鳴を用いた水中病原性微生物バイオセンサ
坂槙有紀恵, 山田健太, 高橋正宏, 岡部聡, 佐藤久, 日本分析化学会年会講演要旨集, 61st, 397, 05 Sep. 2012
Japanese

ジルコニアカラムを用いた極低濃度リン測定法の検討~摩周湖水を対象として~
速水裕人, 深澤達矢, 佐藤久, 高橋正宏, 五十嵐聖貴, 田中敦, 環境化学討論会要旨集(CD-ROM), 21st, ROMBUNNO.3E-07, 10 Jul. 2012
Japanese

重金属センサアレイに向けた変色型ボロンジピロメテン蛍光色素群の開発
菅藤亮輔, 吉川弘晃, 谷山拓生, 羽深昭, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 分析化学討論会講演要旨集, 72nd, 130, 05 May 2012
Japanese

微小電極を用いた堆積型堆肥表層中の一酸化二窒素および一酸化窒素の測定
日向寺崇文, 前田高輝, 岡部聡, 高橋正宏, 佐藤久, 日本水環境学会年会講演集, 46th, 510, 14 Mar. 2012
Japanese

新規変色型蛍光色素を用いた環境サンプル中の重金属イオン分析
谷山拓生, 羽深昭, 菅藤亮輔, 吉川弘晃, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 46th, 506, 14 Mar. 2012
Japanese

新規変色型蛍光色素を用いたクロムイオン分析
吉川弘晃, 羽深昭, 谷山拓生, 菅藤亮輔, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 46th, 676, 14 Mar. 2012
Japanese

都市河川における底生生物相の劣化要因の調査
杉本昌也, 蔭浦一裕, 深澤達矢, 佐藤久, 高橋正宏, 日本水環境学会年会講演集, 46th, 131, 14 Mar. 2012
Japanese

新規変色型蛍光色素を用いた亜鉛イオン分析
羽深昭, 谷山拓生, 菅藤亮輔, 吉川弘晃, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 46th, 622, 14 Mar. 2012
Japanese

環境サンプル測定のためのリン酸マイクロセンサーの開発
宮崎悠爾, 谷内翔, 高橋正宏, 岡部聡, 佐藤久, 化学系学協会北海道支部冬季研究発表会講演要旨集, 2012, 148, 31 Jan. 2012
Japanese

変色型ボロンジピロメテン蛍光色素を用いた重金属イオン分析
菅藤亮輔, 吉川弘晃, 谷山拓生, 羽深昭, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 化学系学協会北海道支部冬季研究発表会講演要旨集, 2012, 101, 31 Jan. 2012
Japanese

東京湾奥部における底泥中の硫化物と酸素消費との関係
牧秀明, 金谷弦, 中村泰男, 管原庄吾, 清家泰, 佐藤久, 日本水環境学会年会講演集, 46th, 2012

新規蛍光色素を用いた重金属イオン分析
羽深昭, 谷山拓生, 菅藤亮輔, 吉川弘晃, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 環境工学研究フォーラム講演集, 48th, 214, 216, 25 Nov. 2011
Japanese

重金属イオン分析に向けた新規蛍光色素の開発
菅藤亮輔, 羽深昭, 谷山拓生, 吉川弘晃, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 環境工学研究フォーラム講演集, 48th, 220, 222, 25 Nov. 2011
Japanese

環境サンプル測定のためのリン酸マイクロセンサーの開発
谷内翔, 宮崎悠爾, 押木守, 佐藤久, 高橋正宏, 岡部聡, 環境工学研究フォーラム講演集, 48th, 217, 219, 25 Nov. 2011
Japanese

PA-034 Anammox細菌Candidatus "Brocadia sinica"の嫌気性アンモニア酸化活性に及ぼす菌体密度の影響(PA 水処理生態系,ポスター発表)
平泉 晴菜, 新家子 香織, 押木 守, 佐藤 久, 岡部 聡, 日本微生物生態学会講演要旨集, 27, 73, 73, 08 Oct. 2011
日本微生物生態学会, Japanese

PA-030 メタゲノム・プロテオームアプローチによる嫌気性アンモニア酸化(anammox)細菌Candidatus 'Brocadia sinica'の生理生態学的特性の解明(PA 水処理生態系,ポスター発表)
押木 守, 新家子 香織, 佐藤 久, 岡部 聡, 日本微生物生態学会講演要旨集, 27, 72, 72, 08 Oct. 2011
日本微生物生態学会, Japanese

部分硝化‐AnammoxプロセスからのN₂O生成
岡部聡, 押木守, 高橋慶考, 佐藤久, 日本水環境学会シンポジウム講演集, 14th, 43, 44, 10 Sep. 2011
Japanese

オンサイト重金属イオン分析に向けた新規変色型蛍光色素の開発
谷山拓生, 羽深昭, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 土木学会年次学術講演会講演概要集(CD-ROM), 66th, ROMBUNNO.VII-192, 05 Aug. 2011
Japanese

新規変色型蛍光色素を用いた水サンプル中重金属イオンの簡易分析
羽深昭, 谷山拓生, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 土木学会年次学術講演会講演概要集(CD-ROM), 66th, ROMBUNNO.VII-191, 05 Aug. 2011
Japanese

環境サンプル測定のためのリン酸マイクロセンサーの開発
宮崎悠爾, 押木守, 高橋正宏, 岡部聡, 佐藤久, 土木学会年次学術講演会講演概要集(CD-ROM), 66th, ROMBUNNO.VII-029, 05 Aug. 2011
Japanese

重金属イオンの識別が可能な変色型ボロンジピロメテン蛍光色素の開発
谷山拓生, 菅藤亮輔, 羽深昭, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本化学会北海道支部夏季研究発表会講演要旨集, 2011, 150, 01 Jul. 2011
Japanese

変色型ボロンジピロメテン蛍光色素を用いた含水サンプル中での重金属イオンの簡易分析
菅藤亮輔, 谷山拓生, 羽深昭, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本化学会北海道支部夏季研究発表会講演要旨集, 2011, 149, 01 Jul. 2011
Japanese

オンサイト重金属イオン分析に向けた新規変色型蛍光色素の開発
谷山拓生, 羽深昭, 菅藤亮輔, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 45th, 293, 18 Mar. 2011
Japanese

都市化による水系生物相劣化の原因
蔭浦一裕, 高橋正宏, 棚池竜也, 杉本昌也, 佐藤久, 深澤達矢, 日本水環境学会年会講演集, 45th, 243, 18 Mar. 2011
Japanese

新規変色型蛍光色素を用いた水サンプル中の重金属検出
菅藤亮輔, 羽深昭, 谷山拓生, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 45th, 295, 18 Mar. 2011
Japanese

新規変色型蛍光色素を用いたレシオメトリー測定による重金属イオン分析
羽深昭, 谷山拓生, 菅藤亮輔, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 45th, 294, 18 Mar. 2011
Japanese

多段型生物処理装置における余剰汚泥減少機能の評価
谷内亜沙美, 松永祐紀, 佐藤久, 渡辺義公, 日本水環境学会年会講演集, 45th, 352, 18 Mar. 2011
Japanese

新規anammox細菌Candidatus ‘Brocadia sinica’における網羅的プロテオーム解析
新家子香織, 押木守, 佐藤久, 岡部聡, 日本水環境学会年会講演集, 45th, 46, 18 Mar. 2011
Japanese

脱気膜を用いた有機性廃水の水素発酵プロセスの高効率化
佐藤久, 池田真之, 高橋正宏, 岡部聡, 笹川学, 中原禎仁, 環境浄化技術, 10, 1, 85, 90, 01 Feb. 2011
Japanese

P-46 Metagenomic analyses for a novel anammox bacterium, Candidatus "Brocadia gluttonica", responsible for high-rate nitrogen removal(Poster Session)
SHINYAKO KAORI, OSHIKI MAMORU, SATOU HISASHI, OKABE SATOSHI, 日本微生物生態学会講演要旨集, 26, 117, 117, 23 Nov. 2010
日本微生物生態学会, English

蛍光色素を用いたレシオメトリー測定による重金属イオン分析
羽深昭, 谷山拓生, 山田幸司, 高橋正宏, 岡部聡, 佐藤久, 環境工学研究フォーラム講演集, 47th, 184, 186, 12 Nov. 2010
Japanese

環境サンプル測定のためのリン酸マイクロセンサーの開発
宮崎悠爾, 押木守, 佐藤久, 高橋正宏, 岡部聡, 環境工学研究フォーラム講演集, 47th, 4, 6, 12 Nov. 2010
Japanese

摩周湖における透明度の低下とその影響因子の検討
高橋岳, 深澤達矢, 宇田川弘勝, 田中敦, 五十嵐聖貴, 小林拓, 濱田浩美, 南尚嗣, 佐藤久, 高橋正宏, 地球環境シンポジウム講演集, 18th, 135, 139, 27 Aug. 2010
土木学会, Japanese

微小電極を用いた放牧地からのN₂O生成速度の測定
佐藤久, 日向寺崇文, 高橋正宏, 岡部聡, 土木学会年次学術講演会講演概要集(CD-ROM), 65th, ROMBUNNO.VII-176, 05 Aug. 2010
Japanese

人口が減少する都市における水循環の再構築
木戸浦茂実, 佐藤久, 高橋正宏, 下水道研究発表会講演集, 47th, 74, 76, 30 Jun. 2010
日本下水道協会, Japanese

森林地域における金属成分の動態
宮川浩樹, 深澤達矢, 野口泉, 高橋正宏, 佐藤久, 福澤加里部, 柴田英昭, 野村睦, 高木健太郎, 三枝信子, 環境化学討論会講演要旨集, 19th, 130, 131, 20 Jun. 2010
Japanese

都市河川における底生生物相の変化とその原因に関する研究
棚池竜也, 高橋正宏, 佐藤久, 深澤達矢, 工藤憲三, 日本水環境学会年会講演集, 44th, 574, 15 Mar. 2010
Japanese

微小電極を用いたANAMMOXグラニュール内の微生物活性の解析
佐藤久, 高橋慶考, 山田陽平, 高橋正宏, 岡部聡, 日本水環境学会年会講演集, 44th, 396, 15 Mar. 2010
Japanese

温泉排水中のヒ素の制御~下水汚泥からの溶出抑制~
清水雄介, 深澤達矢, 佐藤久, 高橋正宏, 環境工学研究フォーラム講演集, 46th, 129, 131, 27 Nov. 2009
Japanese

脱気膜を用いた有機性廃水の水素発酵プロセスの高効率化
池田真之, 佐藤久, 高橋正宏, 岡部聡, 中原禎仁, 笹川学, 環境工学研究フォーラム講演集, 46th, 103, 104, 27 Nov. 2009
Japanese

嫌気性消化プロセスへの脱気膜の適用
佐藤久, 池田真之, 高橋正宏, 岡部聡, 中原禎仁, 笹川学, 用水と廃水, 51, 11, 925, 932, 01 Nov. 2009
産業用水調査会, Japanese

有機性廃棄物からのポリヒドロキシアルカン酸の生産
羽深昭, 佐藤久, 高橋正宏, 岡部聡, 日本水環境学会シンポジウム講演集, 12th, 168, 169, 14 Sep. 2009
Japanese

新規リン酸吸着剤を用いた汚泥からのリン回収
佐藤久, 渡辺義公, 日本水環境学会シンポジウム講演集, 12th, 170, 14 Sep. 2009
Japanese

天塩研究林における大気汚染物質の沈着に関する研究
長谷川祥樹, 深澤達矢, 野口泉, 高橋正宏, 佐藤久, 福澤加里部, 柴田英昭, 野村睦, 高木健太郎, 藤沼康実, 佐々木巴え, 環境化学討論会講演要旨集, 18th, 78, 79, 09 Jun. 2009
Japanese

溶存バイオガスに着目した新規嫌気性廃水処理リアクターの開発
佐藤久, 池田真之, 高橋正宏, 岡部聡, 中原禎仁, 笹川学, 日本水環境学会年会講演集, 43rd, 253, 16 Mar. 2009
Japanese

有機性廃棄物からのポリヒドロキシアルカン酸の生産
羽深昭, 佐藤久, 高橋正宏, 岡部聡, 日本水環境学会年会講演集, 43rd, 623, 16 Mar. 2009
Japanese

有機性廃棄物からのポリヒドロキシアルカン酸の生産
羽深昭, 坂井田健司, 佐藤久, 深澤達矢, 高橋正宏, 岡部聡, 環境工学研究フォーラム講演集, 45th, 61, 63, 28 Nov. 2008
Japanese

溶存バイオガスに着目した嫌気性廃水処理リアクターの開発
佐藤久, 池田真之, 高橋慶多, 深澤達矢, 高橋正宏, 岡部聡, 中原禎仁, 笹川学, 環境工学研究フォーラム講演集, 45th, 58, 60, 28 Nov. 2008
Japanese

脱気膜を用いたメタン発酵プロセスにおける高効率ガス回収
佐藤久, 高橋慶多, 深澤達矢, 高橋正宏, 岡部聡, 中原禎仁, 笹川学, 土木学会年次学術講演会講演概要集(CD-ROM), 63rd, Disk 2, ROMBUNNO.7-043, 13 Aug. 2008
Japanese

水系生物膜内の微生物活性解析への微小電極の適用
佐藤 久, 岡部 聡, 水, 50, 10, 20, 28, Aug. 2008
月刊「水」発行所, Japanese

都市雨水排水中重金属の発生源とその水環境中における化学種の解明
高橋正宏, 佐藤久, 馬場省伍, 下水道研究発表会講演集, 45th, 422, 424, 30 Jun. 2008
日本下水道協会, Japanese

天塩研究林における大気降下物中の金属成分の測定と検討
深澤達矢, 長谷川祥樹, 佐藤久, 高橋正宏, 柴田英昭, 野村睦, 高木健太郎, 藤沼康実, 環境化学討論会講演要旨集, 17th, 214, 215, 10 Jun. 2008
Japanese

有機性廃棄物からのポリヒドロキシアルカン酸の生産
佐藤久, 坂井田健司, 岡部聡, 渡辺義公, 環境バイオテクノロジー学会大会プログラム講演要旨集, 34th, 27, 2008
Japanese

有機性廃棄物からのポリヒドロキシアルカン酸の生産
佐藤久, 坂井田健司, 岡部聡, 渡辺義公, 環境工学研究フォーラム講演集, 44th, 224, 226, 16 Nov. 2007
Japanese

有機性廃棄物からのポリヒドロキシアルカン酸の生産
坂井田健司, 佐藤久, 岡部聡, 渡辺義公, 日本水環境学会年会講演集, 41st, 335, 15 Mar. 2007
Japanese

ジルコニウムメゾ構造体を利用した下水中リンの濃縮・高純度化
半沢拓, 佐藤久, 渡辺義公, 日本水環境学会年会講演集, 41st, 615, 15 Mar. 2007
Japanese

微小電極を用いたUASBグラニュール汚泥内の微生物活性の解析
佐藤久, 對馬育夫, 岡部聡, 環境工学研究フォーラム講演集, 43rd, 2006

UASBグラニュール内の水素濃度分布の解析
佐藤 久, 岡部 聡, 衛生工学シンポジウム論文集, 13, 199, 202, 16 Nov. 2005
北海道大学衛生工学会, Japanese

ヨシ根圏生物膜内の物質濃度分布および微生物群集構造の解析
中村吉志, 渡辺義公, 岡部聡, 佐藤久, 日本水環境学会年会講演集, 39th, 231, 17 Mar. 2005
Japanese

根に形成される生物膜内の物質濃度および微生物群集構造解析
中村吉志, 岡部聡, 渡辺義公, 佐藤久, 日本水環境学会年会講演集, 38th, 508, 17 Mar. 2004
Japanese

A-30 コンクリート腐食の進行に関与する微生物群集構造解析(水処理生態系,口頭発表)
小田切 光典, 伊藤 司, 佐藤 久, 岡部 聡, 日本微生物生態学会講演要旨集, 20, 152, 152, 2004
日本微生物生態学会, Japanese

C-06 ヨシ根圏内の窒素循環と関連細菌群集構造の解析(水圏生態系,口頭発表)
中村 吉志, 岡部 聡, 佐藤 久, 渡辺 義公, 日本微生物生態学会講演要旨集, 20, 208, 208, 2004
日本微生物生態学会, Japanese

微小電極を用いた下水道構造物の腐食機構の検討
佐藤 久, 佐々木 裕一, 小山田 浩之, 岡部 聡, 衛生工学シンポジウム論文集, 11, 69, 72, 31 Oct. 2003
北海道大学衛生工学会, Japanese

コンクリート腐食に関与する微生物群集の構造解析
岡部聡, 小田切光典, 伊藤司, 佐藤久, 小山田浩之, 渡辺義公, 土木学会年次学術講演会講演概要集(CD-ROM), 58th, Disk 2, VII-189, 01 Sep. 2003
Japanese

微好気性生物膜内における硫酸塩還元細菌の空間分布と硫黄の挙動解析
岡部聡, 伊藤司, 佐藤久, 渡辺義公, 用水と廃水, 44, 11, 961, 970, 01 Nov. 2002
Japanese

微小電極を用いたコンクリート表面に形成された生物膜内の硫酸塩還元反応の解析
小山田 浩之, 佐藤 久, 岡部 聡, 伊藤 司, 衛生工学シンポジウム論文集, 10, 109, 112, 31 Oct. 2002
北海道大学衛生工学会, Japanese

河川底泥の窒素循環に及ぼす底生動物の影響
中村 吉志, 佐藤 久, 岡部 聡, 衛生工学シンポジウム論文集, 10, 105, 108, 31 Oct. 2002
北海道大学衛生工学会, Japanese

生物膜内における硫黄酸化反応機構および関連微生物群集の解析
伊藤司, 岡部聡, 渡辺義公, 杉田謙一, 佐藤久, 日本水環境学会年会講演集, 36th, 40, 14 Mar. 2002
Japanese

Kinetic analysis of nitrifying biofilm growing on the rotating membrane disk
K Kimura, Y Watanabe, S Okabe, H Satoh, WATER SCIENCE & TECHNOLOGY: WATER SUPPLY, VOL 1, NO 4, 1, 4, 111, 118, 2001
English

微小電極を用いた生物膜内の溶存酸素輸送機構の解析
佐藤久, 岡部聡, 渡辺義公, 土木学会年次学術講演会講演概要集 第7部, 55th, 114, 115, 31 Aug. 2000
Japanese

混合培養系生物膜内の硝化機構
佐藤久, 山口有希, 岡部聡, 渡辺義公, 日本水環境学会年会講演集, 34th, 158, 15 Mar. 2000
Japanese

微小電極とFISH法を用いた硝化細菌生物膜の生態学的構造と機能の解析
岡部聡, 佐藤久, 渡辺義公, 日本微生物生態学会講演要旨集, 15th, 36, 05 Nov. 1999
Japanese

Nitrification mechanism in biofilm of mixed culture.
佐藤久, 岡部聡, 渡辺義公, 土木学会年次学術講演会講演概要集 第7部, 54th, 428, 429, 01 Aug. 1999
Japanese

Kinetic analysis of biofilm attached to surface of separation membrane.
木村克輝, 渡辺義公, 岡部聡, 佐藤久, 土木学会年次学術講演会講演概要集 第7部, 54th, 430, 431, 01 Aug. 1999
Japanese

Ammonium Oxidation in Mixed-population Biofilms Determined with the Use of Microelectrodes and In Situ Hybridization.
佐藤久, 岡部聡, 渡辺義公, 日本水環境学会年会講演集, 33rd, 3, 189, 214, 15 Mar. 1999
Ammonium oxidation in wastewater biofilms grown on rotating biological contactors (RBCs) was investigated by using microelectrodes and fluorescent in situ hybridization (FISH) technique. The density of ammonia-oxidizers which formed dense cell cluster was about 5 times higher in the innermost biofilm than in the middle and surface of the biofilm. Due to the presence of ammonia-oxidizers throughout the biofilm, ammonium oxidation zone was expanded with increasing in oxygen penetration depth, which consequently resulted in an increase of the overall ammonium flux into the biofilm. Specific ammonium oxidation rate was dependent upon the density of ammonia-oxidizers. Although more ammonia-oxidizers were detected in the innermost layer, they might be in the dormant state and not contribute ammonium oxidation during the RBC operation because of substrate transport limitation. Furthermore, the fraction of O2 consumption for nitrification to total O2 consumption was in the range of 35% -53% in a medium with ammonium as the sole electron donor, indicating the biofilm consumed a significant amount of oxygen for other than nitrification. It implied that heterotrophs would grow and outcompete nitrifiers in biofilms even though there was no organic carbon source in the medium. In conclusion, the combination of microelectrode measurements and FISH technique made it possible to relate the spatial organization of ammonia-oxidizers with their in situ ammonium oxidation rates in biofilms, which obviously providing more insight into the nitrification process in wastewater biofilm systems., Japan Society on Water Environment, Japanese

Microelectrode Measurements of Simultaneous Nitrification and Denitrification in Wastewater Biofilms.
山川岳志, 佐藤久, 岡部聡, 渡辺義公, 日本水環境学会年会講演集, 33rd, 191, 15 Mar. 1999
Japanese

In Situ Analysis of Sulfide Oxidation Mechanism in Microaerophilic Biofilms.
伊藤司, 佐藤久, 岡部聡, 渡辺義公, 日本水環境学会年会講演集, 33rd, 190, 15 Mar. 1999
Japanese

Ammonium Oxidation in Mixed-population Biofilms Determined with the Use of Microelectrodes and in situ Hybridization.
佐藤久, 岡部聡, 渡辺義公, 水環境学会誌, 22, 3, 206, 214, 10 Mar. 1999
Ammonium oxidation in wastewater biofilms grown on rotating biological contactors (RBCs) was investigated by using microelectrodes and fluorescent in situ hybridization (FISH) technique. The density of ammonia-oxidizers which formed dense cell cluster was about 5 times higher in the innermost biofilm than in the middle and surface of the biofilm. Due to the presence of ammonia-oxidizers throughout the biofilm, ammonium oxidation zone was expanded with increasing in oxygen penetration depth, which consequently resulted in an increase of the overall ammonium flux into the biofilm. Specific ammonium oxidation rate was dependent upon the density of ammonia-oxidizers. Although more ammonia-oxidizers were detected in the innermost layer, they might be in the dormant state and not contribute ammonium oxidation during the RBC operation because of substrate transport limitation. Furthermore, the fraction of O2 consumption for nitrification to total O2 consumption was in the range of 35% -53% in a medium with ammonium as the sole electron donor, indicating the biofilm consumed a significant amount of oxygen for other than nitrification. It implied that heterotrophs would grow and outcompete nitrifiers in biofilms even though there was no organic carbon source in the medium. In conclusion, the combination of microelectrode measurements and FISH technique made it possible to relate the spatial organization of ammonia-oxidizers with their in situ ammonium oxidation rates in biofilms, which obviously providing more insight into the nitrification process in wastewater biofilm systems., Japan Society on Water Environment, Japanese

Significance of Substrate C/N Ratio on Structure and Activity of Nitrifying Biofilms Determined by In Situ Hybridization and the Use of Microelectrodes
SATOH Hisashi, OKABE Satoshi, WATANABE Yoshimasa, Journal of Japan Society on Water Environment, 22, 9, 763, 769, 1999
Effects of substrate C/N ratio on the spatial distributions of ammonia-oxidizing bacterial population and their activities in biofilms were investigated by fluorescent in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes and the use of microelectrodes. The increase in substrate C/N ratio (i.e., addition of acetate) immediately intensified the interspecies competition for oxygen between ammonia-oxidizing bacteria and heterotrophic bacteria, and consequently resulted in deterioration of ammonium oxidation rate especially in the surface of the biofilm. This decrease in the ammonium oxidation rate in the surface could explain a steep spatial gradient of ammonia-oxidizing bacterial population within the biofilms cultured at high substrate C/N ratios.Furthermore, development of the spatial distributions of ammonia-oxidizing bacteria and their activities within the biofilm was followed during biofilm development. The active ammonium oxidation zone gradually shifted from the upper layer to the inner layer of the biofilm, which corresponded with the development of the spatial distributions of ammonia-oxidizing bacteria within the biofilm. Based on in situ analyses of microbial structure and activity, we discussed on mechanisms of population dynamics of ammonia-oxidizing bacteria in biofilms., Japan Society on Water Environment, Japanese

Spatial distribution of sulfate-reducing bacteria and behavior of sulfur in microaerophile biofilm.
伊藤司, 佐藤久, 岡部聡, 渡辺義公, 環境工学研究フォーラム講演集, 35th, 147, 149, Nov. 1998
Japanese

Existence form of ammonia oxidation bacteria in municipal sewage biofilm using FISH method.
内藤初夏, 佐藤久, 岡部聡, 渡辺義公, 乗松直生子, 土木学会年次学術講演会講演概要集 第7部, 53rd, 152, 153, Oct. 1998
Japanese

Transition of ammonia oxidation mechanism in mixed bacterial culture biofilm.
佐藤久, 岡部聡, 渡辺義公, 乗松直生子, 土木学会年次学術講演会講演概要集 第7部, 53rd, 154, 155, Oct. 1998
Japanese

Microelectrode Measurements of Sulfate Reduction in Microaerobic Biofilms.
佐藤久, 岡部聡, 伊藤司, 渡辺義公, 水環境学会誌, 21, 6, 367, 375, Jun. 1998
The vertical zonation of 0₂ respiration, NO₃^- respiration, H₂S oxidation and SO₄^2- reduction in microaerobic biofilms grown on rotating biological contactors (RBCs) of treating domestic wastewater was studied by measuring concentration profiles in the biofilms with microelectodes for 0₂, NO₃^-, H₂S and pH. From those profiles, in situ specific reaction rates and substrate fluxes were calculated by using a simple one-dimensional deffusion reaction model and were related to population densities of sulfate-reducing bacteria (SRB) and potential sulfate reduction rates determined by standard batch experiments. The maximum population densities and the highest potential sulfate reduction rates were found in the outermost biofilm. On the contrary, the in situ sulfate reduction rates were restricted in a narrow zone located in the middle biofilm incubated DO=100 μmol·l^-1. An increase in the O₂ and/or NO₃^- penetration depth in the biofilm induced a shift of sulfate reduction zone to the deeper biofilm and a decrease in the specific sulfate reduction rate. This is probably because that the potential sulfate reduction rate decreased toward the depth and addition of nitrate enhanced oxidation of the produced sulfide or increased competition for electron donor with denitrifiers, or both. The microelectrode measurements with the high spatial resolution enable us to quantitatively measure in situ SO₄^2- reduction rate in microaerobic biofilms, which could not be detected measureing sulfide or sulfate flux across the water-biofilm interface., Japan Society on Water Environment, Japanese

微小電極を用いた微好気性生物膜内の硫酸塩還元の測定
佐藤 久, 伊藤 司, 岡部 聡, 渡辺 義公, 衛生工学シンポジウム論文集, 5, 222, 227, 01 Nov. 1997
北海道大学衛生工学会, Japanese

Ecological structure analysis of nitrifying biofilm.
伊藤司, 乗松直生子, 佐藤久, 岡部聡, 渡辺義公, 境一澄, 環境工学研究フォーラム講演集, 34th, 91, 93, Nov. 1997
Japanese

Sulfate reduction and sulphide oxidation in mixed bacterial culture aerobic biofilm.
佐藤久, 岡部聡, 伊藤司, 渡辺義公, 松田尚之, 環境工学研究フォーラム講演集, 34th, 94, 96, Nov. 1997
Japanese

Ecological structure and function of nitrifying bacteria biofilms.
佐藤久, 岡部聡, 渡辺義公, 境一澄, 土木学会年次学術講演会講演概要集 第7部, 52nd, 294, 295, Sep. 1997
Japanese

Effects of flow velocity on biofilm structure in early stage of biofilm formation.
岡部聡, 佐藤久, 渡辺義公, 尾原裕昌, 土木学会年次学術講演会講演概要集 第7部, 52nd, 330, 331, Sep. 1997
Japanese

Microelectrode Measurements of Sulfide in Biofilms at Low Dissolved Oxygen Concentration.
佐藤久, 伊藤司, 岡部聡, 渡辺義公, 日本水環境学会年会講演集, 31st, 136, Mar. 1997
Japanese

Effect of biofilm structure on microorganism activity distribution in aerobic treatment.
佐藤久, 黒田浩史, 岡部聡, 渡辺義公, 土木学会年次学術講演会講演概要集 第7部, 51st, 102, 103, Aug. 1996
Japanese

Methods in Enzymology　　　　　　　　　　　　　　　
A polyphasic approach to study ecophysiology of complex multispecies nitrifying biofilms
Elsevier, 2011, [Joint work]

Nitrification　　　　　　　　　　　　　　　
Nitrification in wastewater treatment
ASM Press, 2010, [Joint work]

ラボスケールDHSろ床を用いた芳香族化合物の分解性能評価と微生物群集構造解析　　　　　　　　　　　　　　　
関口剛生, 佐藤 久, 成廣 隆, 黒田恭平
第58回日本水環境学会年会, 08 Mar. 2024

画像解析による下水処理水中の懸濁物質の特性解析　　　　　　　　　　　　　　　
信時 圭, Kaushalya Uthpala, 中屋佑紀, 佐藤 久
第58回日本水環境学会年会, 08 Mar. 2024

高分子増強－キャピラリー過渡的等速電気泳動法による生体粒子結合型新規DNA アプタマーの探索　　　　　　　　　　　　　　　
松永光司, 佐藤 久, 半田友衣子, 齋藤伸吾
第58回日本水環境学会年会, 08 Mar. 2024

下水道への侵入水に含まれる蛍光性溶存有機物成分の探索　　　　　　　　　　　　　　　
中屋佑紀, 北林里佳子, 田中佑奈, 佐藤 久
第58回日本水環境学会年会, 08 Mar. 2024

マルチオミクス解析による好気的p-トルイル酸分解機構の解明　　　　　　　　　　　　　　　
髙井麻帆, 佐藤 久, 成廣 隆, 黒田恭平
第58回日本水環境学会年会, 08 Mar. 2024, Oral presentation

EPISENS-M法を用いたMBR透過水中ウイルスの定量および活性汚泥法に対する優位性の評価　　　　　　　　　　　　　　　
中祖惟月, 北島正章, 佐藤 久, 木村克輝
第58回日本水環境学会年会, 08 Mar. 2024, Japanese, Oral presentation

北海道の河川における農業・下水処理由来物質に着目した溶存有機物や生物化学的指標の縦断分析　　　　　　　　　　　　　　　
中屋佑紀、赤澤優弥、小野寺岳史郎、宮島龍太、佐藤久、北林里佳子、田中佑奈、相沢智康、大西裕季、熊木康裕、黒田恭平、成廣隆
第57回日本水環境学会年会, 17 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

活性汚泥フロックへの大腸菌吸着に影響を与える因子の検討　　　　　　　　　　　　　　　
石塚祐介、石崎翔大、中屋佑紀、佐藤久
第57回日本水環境学会年会, 16 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

塩素処理が都市河川中の大腸菌数に与える影響　　　　　　　　　　　　　　　
田中佑奈、Mohomed Shayan、中屋佑紀、佐藤久
第57回日本水環境学会年会, 15 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

DHSろ床を用いたPET原料製造廃水処理UASB反応器の後段処理技術の開発　　　　　　　　　　　　　　　
髙井麻帆、中屋佑紀、佐藤久、相沢智康、大西裕季、熊木康裕、成廣隆、黒田恭平
第57回日本水環境学会年会, 15 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

緑色蛍光タンパク質遺伝子導入大腸菌を用いた活性汚泥フロックの大腸菌吸着部位の解明　　　　　　　　　　　　　　　
石崎翔大、石塚祐介、樋口宏介、押木守、中屋佑紀、佐藤久
第57回日本水環境学会年会, 15 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

PCRフリーの金ナノ粒子プローブを用いたメタン生成アーキアと寄生性超微小 細菌の迅速定量技術の開発　　　　　　　　　　　　　　　
半田久純、中島芽梨、中屋佑紀、佐藤久、黒田恭平、成廣隆
第57回日本水環境学会年会, 15 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

Candidatus Patescibacteria／CPRとメタン生成アーキアの共培養系確立への挑戦　　　　　　　　　　　　　　　
中島芽梨、中井亮佑、久保田健吾、中屋佑紀、佐藤久、延優、成廣隆、黒田恭平
第57回日本水環境学会年会, 15 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

培養法とゲノム情報に基づいた嫌気性PETモノマー分解機構の解明　　　　　　　　　　　　　　　
黒田恭平、成廣隆、中屋佑紀、佐藤久、野口太郎、前田稜太
第57回日本水環境学会年会, 15 Mar. 2023
15 Mar. 2023 - 17 Mar. 2023, 松山市

光導波路分光装置を用いた高感度簡易核酸モニタリング法の開発
中島芽梨, 佐藤久
第58回環境工学研究フォーラム, 17 Nov. 2021, Japanese, Poster presentation
16 Nov. 2021 - 18 Nov. 2021, 11819082

Simple assay for spectroscopic quantification of 16S rRNA of ammonia oxidizing bacteria using DNA-modified gold nanoparticles
M. Nakajima, H. Satoh
International Conference on the Challenges in Environmental Science and Engineering (CESE-2021), 06 Nov. 2021, English, Poster presentation
06 Nov. 2021 - 07 Nov. 2021, 11819082

光導波路分光装置を用いた下水処理プロセスの微生物モニタリング
中島芽梨, 佐藤久
日本水環境学会第55回年会, 11 Mar. 2021, Japanese, Oral presentation
10 Mar. 2021 - 12 Mar. 2021, 11819082

Investigation and Troubleshooting of Filamentous bulking in a Wastewater Treatment Plant　　　　　　　　　　　　　　　
Jinming Jia, Hisashi Satoh
Water and Environment Technology Conference Online 2020 (WET2020), 07 Nov. 2020, English, Poster presentation
07 Nov. 2020 - 08 Nov. 2020

Development of a Simple Analytical Method for Determination of Arsenite Using a DNA Aptamer and Gold Nanoparticles
K. Matsunaga, H. Satoh
IWA Water Development Congress & Exhibition 2019, 04 Dec. 2019, English, Poster presentation
01 Dec. 2019 - 06 Dec. 2019, 11819065

蛍光色素修飾DNAと酸化セリウムナノ粒子を用いた簡易ヒ素(V)検出法の開発
松永光司, 佐藤久
日本分析化学会第68年会, 12 Sep. 2019, Japanese, Poster presentation
11 Sep. 2019 - 13 Sep. 2019, 11819065

Development of a Simple Analytical Method for Determination of Arsenate Using FAM-labeled DNA and Cerium Oxide Nanoparticles
K. Matsunaga, H. Satoh
Water and Environment Technology Conference 2019 (WET2019), 13 Jul. 2019, English
13 Jul. 2019 - 14 Jul. 2019, 11819065

Development of a Simple Analytical Method for Determination of Arsenite Using a DNA Aptamer and Gold Nanoparticles
K. Matsunaga, H. Satoh
IWA 9th International Young Water Professionals Conference, 25 Jun. 2019, English, Oral presentation
23 Jun. 2019 - 27 Jun. 2019, 11819065

DNAとナノ粒子を用いた簡易ヒ素検出法の開発　　　　　　　　　　　　　　　
松永 光司, 奥山 優, 平野 麗子, 岡部 聡, 高橋正宏, 佐藤 久
第53回日本水環境学会年会, 07 Mar. 2019, Japanese, Poster presentation
甲府, [Domestic Conference]

新規簡易迅速大腸菌測定法を用いた下水処理水中薬剤耐性大腸菌数の測定　　　　　　　　　　　　　　　
長橋 夏実, 片寄 由貴, 菊地 凱, 平野 麗子, 岡部 聡, 高橋 正宏, 佐藤 久
第58回環境工学研究フォーラム, 18 Dec. 2018, Japanese, Poster presentation
京都, [Domestic Conference]

深層学習を用いた画像認識による 活性汚泥の糸状性バルキングの兆候検出の試み　　　　　　　　　　　　　　　
鈴木 敬介, 山本 雅人, 高橋 正宏, 佐藤久
第57回環境工学研究フォーラム, 18 Dec. 2018, Japanese, Poster presentation
京都, [Domestic Conference]

特定酵素蛍光基質を用いた下水中腸球菌の新規薬剤感受性試験法の開発　　　　　　　　　　　　　　　
岩崎 隼, 平野 麗子, 岡部 聡, 高橋 正宏, 佐藤 久
第56回環境工学研究フォーラム, 18 Dec. 2018, Japanese, Poster presentation
京都, [Domestic Conference]

三価と五価のヒ素を分別定量可能な新規光学的簡易バイオセンサーの開発　　　　　　　　　　　　　　　
松永 光司, 奥山 優, 岡部 聡, 高橋 正宏, 佐藤 久
第55回環境工学研究フォーラム, 18 Dec. 2018, Japanese, Poster presentation
京都, [Domestic Conference]

DNAアプタマーを用いた簡易ヒ素(III)分析手法の開発　　　　　　　　　　　　　　　
松永 光司, 奥山 優, 岡部 聡, 高橋 正宏, 佐藤 久
地下水、土壌汚染とその防止対策に関する研究集会, 30 Oct. 2018, Japanese, Poster presentation
福島, [Domestic Conference]

新規簡易大腸菌数測定法による下水処理水中大腸菌数の網羅的測定　　　　　　　　　　　　　　　
片寄 由貴, 平野 麗子, 高橋 正宏, 岡部 聡, 佐藤 久
下水道研究発表会, 24 Jul. 2018, Japanese, Poster presentation
北九州, [Domestic Conference]

β-グルクロニダーゼを用いた新規簡易大腸菌数測定法の開発　　　　　　　　　　　　　　　
菊地 凱, 片寄 由貴, 平野 麗子, 北島 正章, 高橋 正宏, 岡部 聡, 佐藤 久
下水道研究発表会, 24 Jul. 2018, Japanese, Poster presentation
北九州, [Domestic Conference]

下水処理水中のβ-グルクロニダーゼ活性を利用した簡易迅速大腸菌定量法の開発　　　　　　　　　　　　　　　
佐藤 久, 平野 麗子, 高橋 正宏, 岡部 聡
下水道研究発表会, 24 Jul. 2018, Japanese, Poster presentation
北九州, [Domestic Conference]

水銀イオンとDNAチミン塩基の塩基対形成能を利用した簡易水銀分析法の開発　　　　　　　　　　　　　　　
小林ひかり, 吉原 光, 岡部 聡, 高橋 正宏, 佐藤 久
第52回日本水環境学会年会, 15 Mar. 2018, Japanese, Poster presentation
札幌, [Domestic Conference]

特定酵素蛍光基質を用いた簡易大腸菌数測定の開発　　　　　　　　　　　　　　　
片寄 由貴, 石井 聡, 岡部 聡, 高橋 正宏, 佐藤 久
第52回日本水環境学会年会, 15 Mar. 2018, Japanese, Poster presentation
札幌, [Domestic Conference]

DNAアプタマーを用いた簡易ノロウイルス検出法の開発　　　　　　　　　　　　　　　
吉原 光, 北島 正章, 佐野 大輔, 岡部 聡, 高橋 正宏, 佐藤 久
第52回日本水環境学会年会, 15 Mar. 2018, Japanese, Poster presentation
札幌, [Domestic Conference]

蛍光色素を用いたMRSA簡易測定法の開発　　　　　　　　　　　　　　　
山口 拓郎, 岡部 聡, 高橋 正宏, 佐藤久
第52回日本水環境学会年会, 15 Mar. 2018, Japanese, Poster presentation
札幌, [Domestic Conference]

DNAアプタマーを用いた簡易ヒ素分析法の開発　　　　　　　　　　　　　　　
松永 光司, 奥山 優, 岡部 聡, 高橋 正宏, 佐藤 久
第52回日本水環境学会年会, 15 Mar. 2018, Japanese, Poster presentation
札幌, [Domestic Conference]

下水処理水中のβ-グルクロニダーゼ活性を利用した簡易迅速大腸菌定量法の開発　　　　　　　　　　　　　　　
菊地 凱, 片寄 由貴, 石井 聡, 北島 正章, 岡部 聡, 高橋 正宏, 佐藤 久
第52回日本水環境学会年会, 15 Mar. 2018, Japanese, Poster presentation
札幌, [Domestic Conference]

新規変色型蛍光色素を用いた路面排水中亜鉛濃度の定量　　　　　　　　　　　　　　　
菅藤 亮輔, 羽深 昭, 吉川 弘晃, 大屋 光平, 山田 幸司, 高橋 正宏, 岡部 聡, 佐藤 久
第47回日本水環境学会年会, 11 Mar. 2013, Japanese, Poster presentation
大阪, [Domestic Conference]

環境サンプル測定のためのリン酸マイクロセンサーの開発　　　　　　　　　　　　　　　
宮崎 悠爾, 谷内 翔, 押木 守, 佐藤 久, 高橋 正宏, 岡部 聡
第49回環境工学研究フォーラム, 28 Nov. 2012, Japanese, Poster presentation
京都, [Domestic Conference]

新規蛍光分子プローブを用いた環境水中重金属イオン分析　　　　　　　　　　　　　　　
菅藤 亮輔, 羽深 昭, 吉川 弘晃, 大屋 光平, 山田 幸司, 高橋 正宏, 岡部 聡, 佐藤 久
第49回環境工学研究フォーラム, 28 Nov. 2012, Japanese, Poster presentation
京都, [Domestic Conference]

表面プラズモン共鳴を利用した病原微生物バイオセンサの開発　　　　　　　　　　　　　　　
坂槙 有紀恵, 山田 健太, ピタックティーラム ニティ, 石井 聡, 佐野 大輔, 高橋 正宏, 岡部 聡, 佐藤 久
第49回環境工学研究フォーラム, 28 Nov. 2012, Japanese, Poster presentation
京都, [Domestic Conference]

新規変色型蛍光色素を用いた路面排水中亜鉛濃度の定量　　　　　　　　　　　　　　　
吉川 弘晃, 菅藤 亮輔, 羽深 昭, 山田 幸司, 高橋 正宏, 岡部 聡, 佐藤 久
日本分析化学会第61年会, 19 Sep. 2012, Japanese, Poster presentation
金沢, [Domestic Conference]

トリベンジルチンクロライドを用いたリン酸マイクロセンサーの開発　　　　　　　　　　　　　　　
谷内 翔, 宮崎 悠爾, 高橋 正宏, 岡部 聡, 佐藤 久
日本分析化学会第61年会, 19 Sep. 2012, Japanese, Poster presentation
金沢, [Domestic Conference]

表面プラズモン共鳴を用いた水中病原性微生物バイオセンサ　　　　　　　　　　　　　　　
坂槙 有紀恵, 山田 健太, 高橋 正宏, 岡部 聡, 佐藤 久
日本分析化学会第61年会, 19 Sep. 2012, Japanese, Poster presentation
金沢, [Domestic Conference]

表面プラズモン共鳴を利用した水中病原微生物検出バイオセンサの開発　　　　　　　　　　　　　　　
佐藤久, 坂槙有紀恵, 山田健太, ピタックティーラタムニティ, 石井聡, 佐野大輔, 高橋正宏, 岡部聡
第15回水環境学会シンポジウム, 10 Sep. 2012, Japanese, Poster presentation
佐賀, [Domestic Conference]

重金属センサアレイに向けた変色型ボロンジピロメテン蛍光色素群の開発　　　　　　　　　　　　　　　
菅藤亮輔, 吉川弘晃, 谷山拓生, 羽深 昭, 山田幸司, 高橋正宏, 岡部 聡, 佐藤 久
第72回分析化学討論会, 19 May 2012, Japanese, Poster presentation
鹿児島, [Domestic Conference]

環境サンプル測定のためのリン酸マイクロセンサーの開発　　　　　　　　　　　　　　　
谷内翔, 宮崎悠爾, 高橋正宏, 岡部 聡, 佐藤 久
第46回日本水環境学会年会, 14 Mar. 2012, Japanese, Poster presentation
東京, [Domestic Conference]

微小電極を用いた堆積型堆肥表層中の一酸化二窒素および一酸化窒素の測定　　　　　　　　　　　　　　　
日向寺 崇文, 前田 高輝, 岡部 聡, 佐藤 久
第46回日本水環境学会年会, 14 Mar. 2012, Japanese, Poster presentation
東京, [Domestic Conference]

新規変色型蛍光色素を用いたクロムイオン分析　　　　　　　　　　　　　　　
吉川弘晃, 羽深 昭, 谷山拓生, 菅藤亮輔, 山田幸司, 高橋正宏, 岡部 聡, 佐藤 久
第46回日本水環境学会年会, 14 Mar. 2012, Japanese, Poster presentation
東京, [Domestic Conference]

網羅的重金属イオン分析に向けた新規変色型蛍光色素の開発　　　　　　　　　　　　　　　
菅藤亮輔, 羽深 昭, 谷山拓生, 吉川弘晃, 山田幸司, 高橋正宏, 岡部 聡, 佐藤 久
第46回日本水環境学会年会, 14 Mar. 2012, Japanese, Poster presentation
東京, [Domestic Conference]

新規変色型蛍光色素を用いた環境サンプル中の重金属イオン分析　　　　　　　　　　　　　　　
谷山拓生, 羽深 昭, 菅藤亮輔, 吉川弘晃, 山田幸司, 高橋正宏, 岡部 聡, 佐藤 久
第46回日本水環境学会年会, 14 Mar. 2012, Japanese, Poster presentation
東京, [Domestic Conference]

新規変色型蛍光色素を用いた亜鉛イオン分析　　　　　　　　　　　　　　　
羽深昭, 谷山拓生, 菅藤亮輔, 吉川弘晃, 山田幸司, 高橋正宏, 岡部 聡, 佐藤 久
第46回日本水環境学会年会, 14 Mar. 2012, Japanese, Poster presentation
東京, [Domestic Conference]

水環境保全工学　　　　　　　　　　　　　　　
北海道大学 工学部

水文学　　　　　　　　　　　　　　　
北海道大学 工学部

環境工学実験I　　　　　　　　　　　　　　　
北海道大学 工学部

Water chemistry　　　　　　　　　　　　　　　
北海道大学大学院工学研究院

水質化学特論　　　　　　　　　　　　　　　
北海道大学大学院工学研究院

環境工学概論　　　　　　　　　　　　　　　
北海道大学 工学部

土木環境工学　　　　　　　　　　　　　　　
北海道大学 工学部

分析化学　　　　　　　　　　　　　　　
北海道大学 工学部

Development of pathogen sensors based on the detection of host recognition molecules for the post-PCR era
Grants-in-Aid for Scientific Research
30 Jun. 2023 - 31 Mar. 2025
佐藤 久, 齋藤 伸吾, 中屋 佑紀
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, 23K17767

廃水処理システムの新奇指標微生物「DPANNアーキア」の診断技術開発と実態解明
科学研究費助成事業
01 Apr. 2021 - 31 Mar. 2025
黒田 恭平, 成廣 隆, 延 優, 佐藤 久, 山田 真義, 野口 太郎
本研究の目的は，高有機物負荷，高窒素負荷，高カチオン濃度の廃水処理汚泥中で優占化する「DPANNアーキア」を廃水処理の新奇ストレス指標微生物として診断する技術を開発し，その“真”の機能を培養法とトランスクリプトム解析等により明らかにすることである。「DPANNアーキアは廃水処理のストレスを評価する新奇指標微生物になり得るのではないか?」という問い(仮説)について，培養と情報科学・微生物代謝能の観点から学術的に解明することである。
2021年度は，(1) DPANNアーキア優占化反応槽の運転管理と診断技術の開発，(2) DPANNアーキアの二者培養系の構築の試みの2項目について研究を実施した。(1) において，過去にDPANNアーキアの優占化と処理の不調が発生したフェノール模擬廃水を処理する上昇流嫌気性スラッジブランケット (UASB) 反応槽の16S rRNA遺伝子解析及びショットガンメタゲノム解析を実施し，2種のDPANNアーキアのドラフトゲノムの再構築に成功し，ゲノム情報からヌクレオシドを利用可能な代謝を持つことが明らかとなった。得られた知見を基にしてフェノールを基質とするUASB反応槽のスタートアップを行い，順調に有機物負荷を上昇させることに成功している。金ナノ粒子プローブ法を用いたDPANNアーキアの定量についても検討を進めている。
(2) において，ドラフトゲノムから得られた知見を基にしてDPANNアーキアの集積培養系構築を試みた結果，DPANNアーキアと同様の生理生態を持つと予測されているCandidate Phyla Radiation (CPR)/Patescibacteriaに属する系統の集積培養に成功し，DPANNアーキアの二者培養系確立に向けた宿主と共生細菌の動態に関する重要な情報を得た。加えて本培養系にはDPANNアーキアも維持されていることを確認した。
日本学術振興会, 基盤研究(B), 国立研究開発法人産業技術総合研究所, 21H01471

Development of new water quality sensing technology for realization of sustainable small-scale water supply systems
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
Apr. 2021 - Mar. 2025
佐藤 久, 山田 俊郎, 山村 寛, 黒田 恭平, 齋藤 伸吾
Legionella pneumophilaと結合できるDNAアプタマーを選抜した。選抜は、ポリマー増強キャピラリー過渡的等速電気泳動法（PectI）を用いておこなった。PectIでは、微生物の細胞は移動の初期に過渡的等速電気泳動（tITP）の原理に基づき、狭い単一ピークとなる。tITP積層モードが完了した後プロセスはキャピラリーゾーン電気泳動（CZE）モードに移行し、PectIにおけるピークの分離を行う。分離された単一ピークは、泳動バッファー中に添加されたポリエチレンオキサイド（600,000）（PEO）の効果により、CZEモードの間ずっと維持される。このようにして特異的な結合親和性を有するDNAアプタマーを獲得できる。
今年度は新規のLegionella pneumophila結合DNAアプタマーについて、PectI選抜法によるシングルラウンド選抜と、類似配列群に分けるクラスタリング法とディープラーニングを用いた定量的処理に基づく大規模NGS解析によって選抜することができた。この選抜システムで獲得できたDNAアプタマーの配列は、先行研究における、従来のCell-SELEXで選抜されたDNAアプタマーと比較して低い解離定数および速度定数を持っており、平衡論的、反応速度論的にLegionella pneumophilaと高い結合能を持ったDNAアプタマーを獲得できた。すなわち、先行研究で選抜されたDNAアプタマーよりもLegionella pneumophilaと特異的に結合し、安定した複合体を形成するアプタマーであると考えられ、我々が目標としているバイオセンサーの開発に適したDNAアプタマーを選抜できたと言える。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, Principal investigator, 21H04568

Development and application of innovative monitoring technologies of microorganisms: Challenge to eradication of waterborne infectious diseases in Asia
Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Oct. 2020 - Mar. 2024
佐藤 久, 齋藤 伸吾, 山村 寛, 丁 青
本年度は開発済みの蛍光プローブを用いて、時間的にも空間的にも網羅的に河川水中の大腸菌濃度を測定した。札幌市の創成川の北10条（S1）、北12条（S2）、北14条（S3）、北23条（S4）、北30条（S5）、北49条（S6）の橋から河川水をサンプリングした。創成川水再生プラザからの放流水が流入する地点のすぐ下流に位置するS6においてのみ、大腸菌数が高いことが明らかとなった。公定法で求めた大腸菌数が、本研究室で開発した方法で求めた酵素活性と、大腸菌数が1000 cfu/L以上であれば相関があることがわかった。創成川流下方向の大腸菌数の変化を河川の水位と札幌市の降雨強度と比較した。2月の3回のサンプリングにおいて、創成川水再生プラザの上流（S1からS5）では、大腸菌数は2月16日や22日に高く、2月2日に最も低かった。2月2日は、前日の22時にのみ雨が降っており水位は高くない。このため、ノンポイントソースまたはCSOがなく、処理場上流で大腸菌数が低かったと考えられた。一方、2月16日は、前日から雨が降っており、サンプリング時の水位が高かった。このため、ノンポイントソースまたはCSOにより、処理場上流で大腸菌数が高くなったと考えられた。
核酸を検出する技術を開発した。本技術では病原体の核酸と特異的に結合する2種類のDNAを金ナノ粒子で修飾したDNAプローブを使う。サンプルから抽出したターゲットとDNAプローブを混合する。2つのDNAプローブがターゲットにハイブリダイズする。ターゲットはナノサイズなので2つの金ナノ粒子が互いに近接することになる。金ナノ粒子は近接すると散乱光強度が増強するので、ターゲットの濃度が高いサンプルほど散乱光強度が大きくなり、ターゲット濃度を分析できる。本年度は大腸菌O157の濃度を検出限界10^6 copies/μLで測定できた。
Japan Society for the Promotion of Science, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Hokkaido University, Principal investigator, 20KK0090

Developent of a MFC driven anammox MEC system for self-sustaining wastewater treatment
Grants-in-Aid for Scientific Research
01 Apr. 2019 - 31 Mar. 2023
岡部 聡, 北島 正章, 佐藤 久, 押木 守
本研究の目的は、前段に有機物除去を担うバイオ燃料電池(MFC)を設け、MFCで発生する電圧を用いてMECアノード電極電位を制御することで、アンモニア酸化細菌(AOB)がアノード電極を電子受容体としてNH4+をNO2-まで酸化し、Anammox細菌が生成されたNO2-とNH4+を窒素ガス(N2)へ変換するMFC駆動型部分硝化(PN)-Anammox 生物電解セル(MEC)システムを開発することである。
本年度は昨年度に引き続き、研究開発項目として提示した、①アンモニア酸化細菌（AOB）は、アノード電極を電子受容体としてNH4+をNO2-へ酸化できるか？さらに、②MFCを部分硝化-Anammox MECの補助電源として用いることは可能か？について検討した。複合微生物群集である活性汚泥とNitrosomonas europaeaの純粋株（ATCC19718)を用いて生物電解セル（MEC）を構築し、ポテンシオスタットを用いてMECアノード電極の標準還元電位を+0.8V (vs SHE)に制御し、NH4+および中間生成物であるNH2OHの酸化速度および発生電流量を測定した。また、１５Nでラベルした窒素化合物を添加して気相中のN2, NO, N2OをGC/MSを用いて分析した。その結果、活性汚泥およびNitrosomonas europaeaの両者は、生物電気化学的にNH4+をNH2OHへ酸化することができなかった。一方、NH2OHは電気化学的にN2へ、また、生物学的および電気化学的にN2Oへ変換されることが確認された。以上のことより、既往の研究等で報告されている生物電気化学的アンモニア酸化反応は、リアクターに混入した微量の酸素により、NH4+がNH2OHまで酸化された後、生成したNH2OHが生物電気化学的にN2,またはN2Oへ変換されている可能性を明らかにすることができた。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 19H00776

Development of highly sensitive pathogenic microbial sensors with nano-sized reaction fields
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
Jul. 2021 - Mar. 2023
佐藤 久
今年度は光導波路分光装置を用いて、下水を処理する活性汚泥中の硝化細菌（AOB）のRNA量を測定することに成功した。本手法ではAOBの16S rRNA（AOB-RNAと称す）と特異的に結合する2種類のDNA（プローブDNAと称す）を金ナノ粒子で修飾したAu-プローブと、活性汚泥から抽出したRNAをMgCl2を含むバッファー溶液に添加したものを試料とした。プローブDNAはAOB用のPCRプライマー（CTO189f A/B/CとRT1r）の塩基配列を参考に設計した。都市下水を処理する活性汚泥（A処理場またはB処理場）からRNAを抽出し、AOB-RNA濃度を上述のプライマーを用いて定量的逆転写PCR（RT-qPCR）により定量した。A処理場の活性汚泥またはNitrosomonas europaeaを添加した活性汚泥中のAOB-RNA濃度を測定した。活性汚泥から抽出したAOB-RNA濃度も、N. europaeaを添加した活性汚泥から抽出したAOB-RNA濃度（▲）も、RT-qPCRで測定した濃度と同程度であった。これより本手法では1.8×10の2乗から1.3×10の7乗 copies/μLの範囲で、RT-qPCRで定量した濃度に対する誤差27%から240%でAOB-RNAを定量可能であることがわかった。このように先行研究に比べて定量下限値が極めて低くなった理由として、全細菌とAOBでは使用するプローブDNAの塩基配列が異なりハイブリダイズした際の16S rRNAの立体構造や金ナノ粒子の位置関係が異なること、光導波路分光装置では金ナノ粒子の近接によってシグナル（散乱光）が増強されたことが考えられる。
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, Principal investigator, 21K18737

Development of an non-bio and biological hybrid artificial photosynthesis system for acetate production
Grants-in-Aid for Scientific Research
01 Apr. 2020 - 31 Mar. 2022
Okabe Satoshi
Bio-photoelectrochemical (Bio-PEC) cells have much attention due to their ability to convert solar energy and wastewater energy into electrical or chemical energy. To develop Bio-PECs, three-dimensional heterostructures ZnO/CuO NFRs, ZnO nanorods (NRs) on CuO nanowire (NWs) (ZnO@CuO) with high photoelectrochemical performance were successfully synthesized via thermal oxidation and followed by UV irradiation in pure water at ambient temperature and pressure. The longer UV irradiation time significantly improved the photocurrent generation due to the higher light absorption, larger BET specific surface area, and lower charge recombination opportunity. However, ZnO sputtering was not necessarily. The obtained photocurrent density was about three times higher than the previously reported values for ZnO/CuO composites with a similar three-dimensional nanostructure.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Pioneering), Hokkaido University, 20K20486

蛍光プローブを用いた簡易・迅速・低コストのオンサイト指標細菌一斉計測技術の開発
科学研究費補助金 挑戦的研究（萌芽）
Aug. 2019 - Mar. 2021
佐藤 久
日本学術振興会, Principal investigator, Competitive research funding

IoT技術の基盤となるDNAアプタマーを用いた網羅的水質センサの開発
科学研究費補助金 基盤研究B
Apr. 2017 - Mar. 2020
佐藤 久
日本学術振興会, Principal investigator, Competitive research funding

特定酵素蛍光基質を用いた病原細菌および薬剤耐性細菌の簡易迅速定量法の開発
科学研究費補助金 挑戦的研究（萌芽）
Apr. 2017 - Mar. 2019
佐藤 久
日本学術振興会, Principal investigator, Competitive research funding

執行体制の脆弱化に対応した下水処理水中の細菌・ウイルスの簡易分析技術の開発
下水道技術研究開発（GAIAプロジェクト）
Dec. 2016 - Feb. 2019
佐藤 久
国土交通省, Principal investigator, Competitive research funding

術後眼内炎起因菌の簡易迅速多検体検出キットの開発
研究開発助成事業 イノベーション創出研究支援事業 スタートアップ研究補助金
2016 - Mar. 2017
佐藤 久
ノーステック財団, Principal investigator, Competitive research funding

光導波路分光法を用いたオンサイト・リアルタイム網羅的環境中重金属分析技術の開発
科学研究費補助金 基盤研究B
Apr. 2014 - Mar. 2017
佐藤 久
日本学術振興会, Principal investigator, Competitive research funding

Development of Membrane Bioreactor with less nergy consumption and multi^function
Grants-in-Aid for Scientific Research
21 Oct. 2013 - 31 Mar. 2016
watanabe yoshimasa, KIMURA Katsuki, YAMAMURA Hiroshi, SATOH Hisashi, HAFUKA Akira
A pilot plant study of the buffled MBR with PTFE membranes of 3 m long ,which can achieve simultaneous removal of BOD, T-N and T-P in a single reacto, was carried out. As the results, stabler removal of BOD, T-N and T-P was observed, and the electric power consumption rate of 0.35 kWh/m3 was obtained. It is very low compared with the existing MBR.We developted a nove PTFE hollow fiber MF membrane module with intensive air diffuser. A series of small pilot plant experiment showed a quite low electric power consumption is possible in the MBR with the novel module. P recovery from the MBR exess sludge was possible when the effluent from the MBR was treated through the column filled with zirconia mezo-structured paricles. Anaerobic MBR with a MF hollowfiber membrane incoorated with zirukonium mes- structured particles has a high potential for the phosphate recovery from the excess sludge.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Chuo University, 25249073

近接場光による病原微生物蛍光マルチセンサの超小型・高感度化
科学研究費補助金 挑戦的萌芽研究
Apr. 2014 - Mar. 2016
佐藤 久
日本学術振興会, Principal investigator, Competitive research funding

DNAアプタマープローブによる新しいヒ素モニタリング検査法の開発
マッチングプランナープログラム探索試験
2015 - 2016
佐藤 久
JST, Principal investigator, Competitive research funding

糞便汚染マーカー定量検出を基盤とした微生物学的水質管理手法の確立
科学研究費助成事業
01 Apr. 2011 - 31 Mar. 2014
岡部 聡, 石井 聡, 佐藤 久, 佐野 大輔
現行の糞便汚染指標である大腸菌群数による微生物学的水質管理は、水環境中での大腸菌群の増殖、糞便に由来しない大腸菌群の存在など、数多くの問題点が指摘されている。このため、合理的な糞便性汚染指標の確立および水域の微生物学的水質管理手法の確立が急務となっている。このような背景のもと、本研究では、宿主特異的遺伝子マーカー（腸内蛋白質分解細菌の最優占種であるBacteroides-Prevotella 属由来遺伝子）をもとに水域の糞便汚染レベルを定量的に評価し、さらに糞便汚染源（ヒト、家畜及び野生動物等）を迅速かつ正確に特定する新規方法論を確立し、具体的な汚染防止対策の構築を含む合理的な微生物学的水質管理を実現することを目的としている。
本年度は、昨年度開発した各宿主特異的糞便汚染マーカーを実際の水環境に適用し、糞便汚染の実態を明らかにした。さらに、遺伝子マーカーを糞便汚染指標として活用するためには、生存細胞と死細胞を区別して定量することが求められる。そこで、Propidium monoazide (PMA)を併用した定量PCR法を確立し、糞便汚染源の特定を行うために重要となる生菌由来の糞便性汚染マーカーの定量が可能となった。
次に、環境水中における糞便汚染マーカー（ヒト、ブタ、ウシ、ニワトリ、カモの各宿主特異的遺伝子マーカー）の挙動を解析した。さらに、既存の糞便汚染指標である大腸菌群数、糞便生大腸菌群及び大腸菌の定量も行い、減衰速度の違いを評価した。
最後に、水系感染する腸管系感染症起因細菌及びノロウイルス等の腸管系ウイルスの特異的検出を行い、各宿主特異的遺伝子マーカーと病原微生物の環境水中における存在比の相関関係を調査した。
日本学術振興会, 基盤研究(A), 北海道大学, 23246094

Development of a surface plasmon resonance sensorfor high throughput screening of O157
Grants-in-Aid for Scientific Research
Apr. 2012 - Mar. 2014
SATOH Hisashi, ISHII Satoshi
Escherichia coli (E. coli) can contaminate drinking water, posing a significant threat to public health safety. In order to prevent such serious health problems, identification and quantification of the bacteria is of great importance. At present, various promising approaches have been introduced over the past decade to identify and quantify pathogens in water samples. However, these existing techniques are complex and require enrichment, fluorescent labelling and longer time to obtain results. Thus, rapid and easy-to-use screening tools of pathogens in water samples are still needed in field settings or even at home. The objective of this study is to develop a SPR biosensor for rapid detection of E. coli O157:H7 in liquid samples. We could determined E. coli O157:H7 within 20 min. the detection limit of the sensor was 10 to the 8th power cells/mL. Glycine-HCl solution regenerated the sensor.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 24656306

水中亜鉛の現場分析法の開発と水生生物生態影響機構の解明
環境助成研究
2013 - 2014
佐藤 久
公益財団法人鉄鋼環境基金, Principal investigator, Competitive research funding

水環境保全と再生水安全性確保のためのマルチ重金属センサの開発
技術研究助成
2014
佐藤 久
公益財団法人JFE21世紀財団, Principal investigator, Competitive research funding

北方圏に適した省エネ・創エネ型汚水処理プロセスの開発　　　　　　　　　　　　　　　
北海道ガス大学研究支援制度
Apr. 2011 - Mar. 2012
佐藤 久
北海道ガス, Principal investigator, Competitive research funding

表面プラズモン共鳴による水中病原ウイルスセンサの開発　　　　　　　　　　　　　　　
環境研究助成
2011 - 2012
佐藤 久
住友財団, Principal investigator, Competitive research funding

Development of onsite multi analysis system for ions in aquatic samples based on novel fluoroionophores
Grants-in-Aid for Scientific Research
2011 - 2012
SATOH Hisashi
Fluorescence spectroscopy has great potential for on-site and real-time monitoring of pollutants in aquatic environments; however, its application to environmental aquatic samples has been extremely limited. In this study, a novel fluoroionophore based on a BODIPY-terpyridine conjugate was developed and applied to determine Zn concentrations in urban runoff. The fluoroionophore selectively bound to Zn2+ in water. Zn concentrations could be quantified using the ratio of fluorescence intensities, and the detection limit was 9 ug/L, which is sufficiently low for environmental aquatic samples. To demonstrate applicability of the method to environmental samples, we measured Zn concentrations in urban runoff samples with a complex matrix. The total and dissolved fractions of Zn in the samples could be determined by fluorescence spectroscopy and its relative error was estimated to be less than 30% by inductively coupled plasma-atomic emission spectroscopy analysis.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (A), Hokkaido University, Principal investigator, Competitive research funding, 23686074

Development of a sensor for detection of pathogenic virus in water based on fluorescent molecule and virus binding protein
Grants-in-Aid for Scientific Research
2010 - 2011
SATOH Hisashi, SANO Daisuke
The final goal of this study is to develop a sensor for detection of pathogenic virus in water. In this study, we synthesized virus-binding proteins(VBP) and fluorophore. VBP were produced by genetically-modified E. coli. The SDS-PAGE profile showed the extracted soluble proteins from E. coli cells were VBP of which the estimated molecular weight is 57 kDa. We could also synthesize fluorophore(4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene) in our laboratory
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 22656115

ゼロエミッション・高資源回収型下水汚泥処理プロセスの開発
建設技術研究開発助成制度
Apr. 2007 - Mar. 2010
佐藤 久
国土交通省, Principal investigator, Competitive research funding

Stabilization of super high-rate anammox reactors and analysis of thecommunity structure and function.
Grants-in-Aid for Scientific Research(若手研究(B))
2007 - 2008
Tomonori KINDACHI, 佐藤 久, 笠原 伸介
アナモックスリアクターの長期安定化をはかるために、実排水を想定した低分子有機酸存在下でのアナモックス活性の評価を行った。その結果、酢酸およびプロピオン酸はアナモックス活性に影響を与えないことが明らかとなった。本リアクター内には二種類のアナモックス細菌が存在し、その一方は有機酸に耐性のある種に近縁であった。リアクター内に共存する他栄養細菌は放射性同位元素を用いて有機物の追跡を行ったところ、アナモックス細菌由来の有機物質を分解しており、系内に有機物の蓄積を防ぐ役割をもつことが示唆された。
Ministry of Education, Culture, Sports, Science and Technology, 若手研究(B), 広島大学, Coinvestigator not use grants, Competitive research funding, 19760373

様々な自然環境における温室効果ガス生成機構の解明を目的としたマイクロセンサの開発
科学研究費補助金(若手研究(B))
2006 - 2007
佐藤 久
現在までの多数の研究により、温室効果ガス(GHG)の排出が地球温暖化を促進することは明らかであるが、自然界におけるGHG消長過程の詳細については明らかにされていない。そこで本研究ではGHGの一つであるメタンガス(CH_4)測定用マイクロセンサを開発すること、これを用いて嫌気性廃水処理リアクターで生成されているCH_4濃度を測定することを目的とした。H18年度にCH_4測定用マイクロセンサを開発した。H19年度はCH_4測定用マイクロセンサの検量線を作成した。作製した全てのCH_4測定用マイクロセンサは、検出電流値がCH_4濃度に比例して変化した。代表的なマイクロセンサのCH_4濃度と検出電流値の近似曲線はy=-205x+5194であり、R^2=0.9633と相関は高かった。応答時間は濃度変化が大きい場合は数分、嫌気性廃水処理リアクター内に形成された微生物集塊(グラニュール)内のCH_4濃度を測定する場合には約60秒であった。これらマイクロセンサを用いてグラニュール内の濃度プロファイルを測定した。その結果、H_2生成反応およびH_2消費反応はともにグラニュール表層(厚さ数100μm)で、CH_4生成反応は中層および深層で生じていることが明らかとなった。さらに、グラニュール汚泥の培養条件(酢酸、H_2および炭酸濃度)を変化させて内部のH_2およびCH_4濃度を測定した。酢酸濃度を...
文部科学省, 若手研究(B), 八戸工業大学->北海道大学, Principal investigator, Competitive research funding, 18710013

生物処理装置及び生物処理方法
Patent right, 佐藤 久, 中原 禎仁, 笹川 学, 国立大学法人 北海道大学, 三菱レイヨン・エンジニアリング株式会社
特願2008-025668, 05 Feb. 2008
特開2009-183848, 20 Aug. 2009
200903044735059460

ポーラログラフ式電極のゲル状電解液およびその作成方法
Patent right, 佐藤 久, 安川 基行, 財団法人青森県工業技術教育振興会
特願2005-252576, 05 Aug. 2005
特開2007-047135, 22 Feb. 2007
200903049090274656

排水処理方法
Patent right, 小林 真澄, 加茂 純, 佐藤 久, 三菱レイヨン株式会社
特願2003-274523, 15 Jul. 2003
特開2005-034739, 10 Feb. 2005
200903030123323620