Yamazaki Tomoya
| Institute of Low Temperature Science Frontier Ice and Snow Science | Associate Professor |
Last Updated :2025/11/06
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- Master's degree program, Graduate School of Science
- Doctoral (PhD) degree program, Graduate School of Science
■Research activity information
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Papers
- Creation of lipid whisker crystals for stable oleogel preparation
Koizumi, H., Nakano, F., Oishi, N., Yamazaki, T., Kimura, Y., Tsuda, S., Ueno, S.
Food Research International, 214, 2025
Scientific journal - Transmission Electron Microscopy Imaging of Cluster Unit Dissolution from a Single Crystal of Tetra-n-butylammonium Fluoride Semiclathrate Hydrate for Latent-Heat Storage Material
Machida, H., Sugahara, T., Hata, H., Ueda, T., Yamazaki, T., Kimura, Y.
ACS Applied Nano Materials, 8, 12, 2025
Scientific journal - Micrometer-Scale Graphene-Based Liquid Cells of Highly Concentrated Salt Solutions for In Situ Liquid-Cell Transmission Electron Microscopy.
Yuga Yashima, Tomoya Yamazaki, Yuki Kimura
ACS omega, 9, 38, 39914, 39924, 24 Sep. 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, In situ liquid-cell transmission microscopy has attracted much attention as a method for the direct observations of the dynamics of soft matter. A graphene liquid cell (GLC) has previously been investigated as an alternative to a conventional SiN x liquid cell. Although GLCs are capable of scavenging radicals and providing high spatial resolutions, their production is fundamentally stochastic, and a significant compositional change in liquids encapsulated in GLCs has recently been pointed out. We found that graphene-based liquid cells were formed in nano- to micrometer sizes with high reproducibility when the concentration of the encapsulated aqueous salt solution was high. In contrast, when we revisited conventional fabrication methods, water-encapsulated GLC was formed with low yield, and any electron diffraction spots from ice were not confirmed by a cooling experiment. The reason for this was the presence of intrinsic defects in the graphene, the presence of which we confirmed by the etch-pit method. The shrinkage of a water-encapsulated cell and a decrease in the bubble area in an aqueous (NH4)2SO4 solution cell suggested that volatile water molecules and gas molecules can leak from the cells during the fabrication and observation processes. Further revision of the conditions for the formation of liquid cells and a reduction in the number of intrinsic graphene defects are expected to lead to the provision of graphene-based liquid cells capable of encapsulating dilute aqueous solutions or pure water. - Dependence of Homoimmiscible Water Dynamics on Overpressure at the Interface between Water and the Basal Plane of Single-Crystal Ice Ih
Hiromasa Niinomi, Hiroki Nada, Tomoya Yamazaki, Tetsuya Hama, Akira Kouchi, Tomoya Oshikiri, Masaru Nakagawa, Yuki Kimura
The Journal of Physical Chemistry C, American Chemical Society (ACS), 09 Sep. 2024, [Peer-reviewed], [Invited]
Scientific journal - In-Situ Observation of DL-Alanine Crystallization from a Laser-Trapped Dense Liquid Droplet as a Heterogeneous Nucleation Site
Hiromasa Niinomi, Hiroshi Y Yoshikawa, Ryuzo Kawamura, Tomoya Yamazaki, Tomoya Oshikiri, Masaru Nakagawa
Chemistry Letters, Oxford University Press (OUP), 20 May 2024, [Peer-reviewed]
Scientific journal, Abstract
Nucleation from an aqueous solution is an important step in crystallization which controls the physicochemical properties of crystalline materials. Although dense liquid droplets are considered as a precursor of a crystal in the two-step nucleation model, their actual role is unclear. Our in-situ microscopic observations of the crystallization of DL-alanine from a dense liquid droplet trapped by laser tweezers show that the liquid droplets play the role of a substrate facilitating heterogeneous nucleation rather than a precursor of a crystal. - Enantioselective three-dimensional high-performance liquid chromatographic determination of amino acids in the Hayabusa2 returned samples from the asteroid Ryugu
Aogu Furusho, Chiharu Ishii, Takeyuki Akita, Mai Oyaide, Masashi Mita, Hiroshi Naraoka, Yoshinori Takano, Jason P. Dworkin, Yasuhiro Oba, Toshiki Koga, Kazuhiko Fukushima, Dan Aoki, Minako Hashiguchi, Hajime Mita, Yoshito Chikaraishi, Naohiko Ohkouchi, Nanako O. Ogawa, Saburo Sakai, Daniel P. Glavin, Jamie E. Elsila, Eric T. Parker, José C. Aponte, Hannah L. McLain, Francois Regis Orthous-Daunay, Véronique Vuitton, Roland Thissen, Cédric Wolters, Philippe Schmitt-Kopplin, Alexander Ruf, Junko Isa, Norbert Hertkorn, John M. Eiler, Toshihiro Yoshimura, Haruna Sugahara, Heather V. Graham, Yoshihiro Furukawa, Daisuke Araoka, Satoru Tanaka, Takaaki Yoshikawa, Fumie Kabashima, Kazunori Sasaki, Hajime Sato, Tomoya Yamazaki, Morihiko Onose, Mayu Morita, Yuki Kimura, Kuniyuki Kano, Junken Aoki, Kosuke Fujishima, Shin ichiro Nomura, Shogo Tachibana, Tomoki Nakamura, Takaaki Noguchi, Ryuji Okazaki, Hikaru Yabuta, Hisayoshi Yurimoto, Kanako Sakamoto, Toru Yada, Masahiro Nishimura, Aiko Nakato, Akiko Miyazaki, Kasumi Yogata, Masanao Abe, Tomohiro Usui, Makoto Yoshikawa, Takanao Saiki, Satoshi Tanaka, Fuyuto Terui, Satoru Nakazawa, Tatsuaki Okada, Sei ichiro Watanabe, Yuichi Tsuda, Kenji Hamase
Journal of Chromatography Open, 5, May 2024, [Peer-reviewed]
Scientific journal - Machine Learning Refinement of In Situ Images Acquired by Low Electron Dose LC-TEM.
Hiroyasu Katsuno, Yuki Kimura, Tomoya Yamazaki, Ichigaku Takigawa
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 29 Jan. 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, We have studied a machine learning (ML) technique for refining images acquired during in situ observation using liquid-cell transmission electron microscopy. Our model is constructed using a U-Net architecture and a ResNet encoder. For training our ML model, we prepared an original image dataset that contained pairs of images of samples acquired with and without a solution present. The former images were used as noisy images, and the latter images were used as corresponding ground truth images. The number of pairs of image sets was 1,204, and the image sets included images acquired at several different magnifications and electron doses. The trained model converted a noisy image into a clear image. The time necessary for the conversion was on the order of 10 ms, and we applied the model to in situ observations using the software Gatan DigitalMicrograph (DM). Even if a nanoparticle was not visible in a view window in the DM software because of the low electron dose, it was visible in a successive refined image generated by our ML model. - Chiral Spinodal-like Ordering of Homoimmiscible Water at Interface between Water and Chiral Ice III
Hiromasa Niinomi, Tomoya Yamazaki, Hiroki Nada, Tetsuya Hama, Akira Kouchi, Tomoya Oshikiri, Masaru Nakagawa, Yuki Kimura
The Journal of Physical Chemistry Letters, 15, 2, 659, 664, American Chemical Society (ACS), 11 Jan. 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, Diversity in structures of water endowed by a hydrogen-bonding network plays crucial roles in wide varieties of phenomena in nature. Chiral ordering of water molecules is an intriguing phenomenon from the viewpoint of bimolecular functions. However, experimental reports on chiral ordering have been limited to the water molecules interacting with biomolecules on the molecular scale. It remains unclear whether pure liquid water forms long-range chiral ordering without any interaction with biomolecules. Here, we show that chiral anisotropy can be observed in the macro/mesoscopic network pattern of an unknown water layer formed via spinodal phase separation-like dynamics at the interface between water and ice III with a chiral crystal structure. We named this unknown water homoimmiscible water. Our observations infer that the unknown water is a chiral liquid crystal. This possibility opens new avenues for a wide variety of research fields such as liquid polymorphism, biology, earth and planetary science, and so forth from the perspective of chirality. - Polycyclic aromatic hydrocarbons in samples of Ryugu formed in the interstellar medium
Sarah S. Zeichner, José C. Aponte, Surjyendu Bhattacharjee, Guannan Dong, Amy E. Hofmann, Jason P. Dworkin, Daniel P. Glavin, Jamie E. Elsila, Heather V. Graham, Hiroshi Naraoka, Yoshinori Takano, Shogo Tachibana, Allison T. Karp, Kliti Grice, Alex I. Holman, Katherine H. Freeman, Hisayoshi Yurimoto, Tomoki Nakamura, Takaaki Noguchi, Ryuji Okazaki, Hikaru Yabuta, Kanako Sakamoto, Toru Yada, Masahiro Nishimura, Aiko Nakato, Akiko Miyazaki, Kasumi Yogata, Masanao Abe, Tatsuaki Okada, Tomohiro Usui, Makoto Yoshikawa, Takanao Saiki, Satoshi Tanaka, Fuyuto Terui, Satoru Nakazawa, Sei-ichiro Watanabe, Yuichi Tsuda, Kenji Hamase, Kazuhiko Fukushima, Dan Aoki, Minako Hashiguchi, Hajime Mita, Yoshito Chikaraishi, Naohiko Ohkouchi, Nanako O. Ogawa, Saburo Sakai, Eric T. Parker, Hannah L. McLain, Francois-Regis Orthous-Daunay, Véronique Vuitton, Cédric Wolters, Philippe Schmitt-Kopplin, Norbert Hertkorn, Roland Thissen, Alexander Ruf, Junko Isa, Yasuhiro Oba, Toshiki Koga, Toshihiro Yoshimura, Daisuke Araoka, Haruna Sugahara, Aogu Furusho, Yoshihiro Furukawa, Junken Aoki, Kuniyuki Kano, Shin-ichiro M. Nomura, Kazunori Sasaki, Hajime Sato, Takaaki Yoshikawa, Satoru Tanaka, Mayu Morita, Morihiko Onose, Fumie Kabashima, Kosuke Fujishima, Tomoya Yamazaki, Yuki Kimura, John M. Eiler
Science, 382, 6677, 1411, 1416, American Association for the Advancement of Science (AAAS), 22 Dec. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Polycyclic aromatic hydrocarbons (PAHs) contain ≲20% of the carbon in the interstellar medium. They are potentially produced in circumstellar environments (at temperatures ≳1000 kelvin), by (~10 kelvin) interstellar clouds, or by processing of carbon-rich dust grains. We report isotopic properties of PAHs extracted from samples of the asteroid Ryugu and the meteorite Murchison. The doubly- 13 C substituted compositions (Δ2× 13 C values) of the PAHs naphthalene, fluoranthene, and pyrene are 9 to 51‰ higher than values expected for a stochastic distribution of isotopes. The Δ2× 13 C values are higher than expected if the PAHs formed in a circumstellar environment, but consistent with formation in the interstellar medium. By contrast, the PAHs phenanthrene and anthracene in Ryugu samples have Δ2× 13 C values consistent with formation by higher-temperature reactions. - In Situ Transmission Electron Microscopy Study of Bubble Behavior Near the Surface of Ice Crystals by Using a Liquid Cell With a Peltier Cooling Holder.
Tomoya Yamazaki, Yuga Yashima, Hiroyasu Katsuno, Hiroya Miyazaki, Takashi Gondo, Yuki Kimura
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 29, 6, 1940, 1949, 21 Dec. 2023, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal, Liquid cell transmission electron microscopy (LC-TEM) is a unique technique that permits in situ observations of various phenomena in liquids with high spatial and temporal resolutions. One difficulty with this technique is the control of the environmental conditions in the observation area. Control of the temperature ranging from room temperature to minus several tens of degrees Celsius, is desirable for controlling the supersaturation in various materials and for observing crystallization more easily. We have developed a cooling transmission electron microscopy specimen holder that uses Peltier devices, and we have combined it with a liquid cell to realize accurate temperature control in LC-TEM. We evaluated this system by using water as a specimen. Motionless bubbles, shown to be voids containing pressurized gas, formed in the specimen sometime after the temperature had reached -12°C. An electron diffraction pattern showed that the specimen turned into ice Ih after the formation of these bubbles, confirming that our system works properly and can induce crystallization. In addition, we analyzed the behavior of bubbles formed in the ice Ih, and we discussed the formation of these bubbles and their internal pressure. - 機械学習によるデータ駆動型の液中透過型電子顕微鏡“その場”観察
Yuki KIMURA, Hiroyasu KATSUNO, Shizuka HIRAKAWA, Tomoya YAMAZAKI
Vacuum and Surface Science, 66, 12, 700, 705, Surface Science Society Japan, 10 Dec. 2023, [Peer-reviewed]
Scientific journal - Dissolution enables dolomite crystal growth near ambient conditions.
Joonsoo Kim, Yuki Kimura, Brian Puchala, Tomoya Yamazaki, Udo Becker, Wenhao Sun
Science (New York, N.Y.), 382, 6673, 915, 920, 24 Nov. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Crystals grow in supersaturated solutions. A mysterious counterexample is dolomite CaMg(CO3)2, a geologically abundant sedimentary mineral that does not readily grow at ambient conditions, not even under highly supersaturated solutions. Using atomistic simulations, we show that dolomite initially precipitates a cation-disordered surface, where high surface strains inhibit further crystal growth. However, mild undersaturation will preferentially dissolve these disordered regions, enabling increased order upon reprecipitation. Our simulations predict that frequent cycling of a solution between supersaturation and undersaturation can accelerate dolomite growth by up to seven orders of magnitude. We validated our theory with in situ liquid cell transmission electron microscopy, directly observing bulk dolomite growth after pulses of dissolution. This mechanism explains why modern dolomite is primarily found in natural environments with pH or salinity fluctuations. More generally, it reveals that the growth and ripening of defect-free crystals can be facilitated by deliberate periods of mild dissolution. - In situ cryogenic transmission electron microscopy observation on the formation of hydrogen-ordered hexagonal ices and its astrophysical implications
Tomoya Yamazaki, Akira Kouchi, Ken-ichiro Murata, Hiroyasu Katsuno, Hiroki Nada, Tetsuya Hama, Yuki Kimura
Monthly Notices of the Royal Astronomical Society, Oxford University Press (OUP), 06 Nov. 2023, [Peer-reviewed], [Lead author, Corresponding author]
Scientific journal, Abstract
Water ices play a critical role in various astrophysical phenomena and store information about the thermal history of icy grains. To understand this history, clarifying the formation conditions in astrophysical environments is critical. In addition to taking the form of amorphous ices, ice can take four crystalline forms: hexagonal ice I (ice Ih), cubic ice I (ice Ic) and hydrogen-ordered hexagonal ice (ice XIh) and cubic ice (ice XIc). The aim of this article is to study the conditions for phase transitions among these different crystalline ices. We found through cryogenic transmission electron microscopy observations that some polymorphs of ice XIh were formed by simple annealing of ice Ih at 120–150 K without any dopant or irradiation. We also investigated the formation conditions of ice Ih by vapor deposition and heating of ice Ic in protoplanetary disks. The transition kinetics from ice Ih to ice XIh suggested that ice XIh exists between the transition temperature from ice Ic to ice Ih and 150 K. From these results, we constructed a water-vapor flux–temperature–time diagram that clarifies the amorphous, Ic, Ih, XIc and XIh ice phases. We found that, under the conditions encountered in most protostars, hydrogen-ordered ices XIh and XIc can exist just outside the snow line and below 125 K, respectively. The infrared libration modes should enable these phases to be distinguished observationally. Because some of these phases are chiral, their presence could fundamentally affect the evolution of other homochiral organic molecules that develop on their surface. - Anisotropy in spinodal-like dynamics of unknown water at ice V–water interface
Hiromasa Niinomi, Tomoya Yamazaki, Hiroki Nada, Tetsuya Hama, Akira Kouchi, Tomoya Oshikiri, Masaru Nakagawa, Yuki Kimura
Scientific Reports, 13, 1, 16227, 16227, Springer Science and Business Media LLC, 11 Oct. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Abstract
Experimentally demonstrating the existence of waters with local structures unlike that of common water is critical for understanding both the origin of the mysterious properties of water and liquid polymorphism in single component liquids. At the interfaces between water and ices Ih, III, and VI grown/melted under pressure, we previously discovered low- and high-density unknown waters, that are immiscible with the surrounding water. Here, we show, by in-situ optical microscopy, that an unknown water appears at the ice V–water interface via spinodal-like dynamics. The dewetting dynamics of the unknown water indicate that its characteristic velocity is ~ 90 m/s. The time evolution of the characteristic length of the spinodal-like undulation suggests that the dynamics may be described by a common model for spinodal decomposition of an immiscible liquid mixture. Spinodal-like dewetting dynamics of the unknown water transiently showed anisotropy, implying the property of a liquid crystal. - Chemical evolution of primordial salts and organic sulfur molecules in the asteroid 162173 Ryugu
Toshihiro Yoshimura, Yoshinori Takano, Hiroshi Naraoka, Toshiki Koga, Daisuke Araoka, Nanako O. Ogawa, Philippe Schmitt-Kopplin, Norbert Hertkorn, Yasuhiro Oba, Jason P. Dworkin, José C. Aponte, Takaaki Yoshikawa, Satoru Tanaka, Naohiko Ohkouchi, Minako Hashiguchi, Hannah McLain, Eric T. Parker, Saburo Sakai, Mihoko Yamaguchi, Takahiro Suzuki, Tetsuya Yokoyama, Hisayoshi Yurimoto, Tomoki Nakamura, Takaaki Noguchi, Ryuji Okazaki, Hikaru Yabuta, Kanako Sakamoto, Toru Yada, Masahiro Nishimura, Aiko Nakato, Akiko Miyazaki, Kasumi Yogata, Masanao Abe, Tatsuaki Okada, Tomohiro Usui, Makoto Yoshikawa, Takanao Saiki, Satoshi Tanaka, Fuyuto Terui, Satoru Nakazawa, Sei-ichiro Watanabe, Yuichi Tsuda, Shogo Tachibana, Kenji Hamase, Aogu Furusho, Kazuhiko Fukushima, Dan Aoki, Daniel P. Glavin, Hannah L. McLain, Jamie E. Elsila, Heather V. Graham, John M. Eiler, Alexander Ruf, Francois-Regis Orthous-Daunay, Cédric Wolters, Junko Isa, Véronique Vuitton, Roland Thissen, Haruna Sugahara, Hajime Mita, Yoshihiro Furukawa, Yoshito Chikaraishi, Mayu Morita, Morihiko Onose, Fumie Kabashima, Kosuke Fujishima, Hajime Sato, Kazunori Sasaki, Kuniyuki Kano, Shin-ichiro M. Nomura, Junken Aoki, Tomoya Yamazaki, Yuki Kimura
Nature Communications, 14, 1, Springer Science and Business Media LLC, 18 Sep. 2023, [Peer-reviewed]
Scientific journal, Abstract
Samples from the carbonaceous asteroid (162173) Ryugu provide information on the chemical evolution of organic molecules in the early solar system. Here we show the element partitioning of the major component ions by sequential extractions of salts, carbonates, and phyllosilicate-bearing fractions to reveal primordial brine composition of the primitive asteroid. Sodium is the dominant electrolyte of the salt fraction extract. Anions and NH4+ are more abundant in the salt fraction than in the carbonate and phyllosilicate fractions, with molar concentrations in the order SO42− > Cl− > S2O32− > NO3− > NH4+. The salt fraction extracts contain anionic soluble sulfur-bearing species such as Sn-polythionic acids (n < 6), Cn-alkylsulfonates, alkylthiosulfonates, hydroxyalkylsulfonates, and hydroxyalkylthiosulfonates (n < 7). The sulfur-bearing soluble compounds may have driven the molecular evolution of prebiotic organic material transforming simple organic molecules into hydrophilic, amphiphilic, and refractory S allotropes. - Observation of hydrogen-ordered cubic ice thin films on the surface of ice Ic nanocrystals upon coarsening
Akira Kouchi, Tomoya Yamazaki, Hiroyasu Katsuno, Hiroki Nada, Tetsuya Hama, Yuki Kimura
Chemical Physics, 572, 111966, 111966, Elsevier BV, Aug. 2023, [Peer-reviewed]
Scientific journal - Chiral Ice Crystals in Space
Akira Kouchi, Takashi Shimonishi, Tomoya Yamazaki, Masashi Tsuge, Naoki Nakatani, Kenji Furuya, Hiromasa Niinomi, Yasuhiro Oba, Tetsuya Hama, Hiroyasu Katsuno, Naoki Watanabe, Yuki Kimura
Chirality - New Insights [Working Title], IntechOpen, 22 Aug. 2022, [Peer-reviewed], [Invited]
In book, We observed the formation of CO, CH3OH, and H2O ices using a cryogenic transmission electron microscope, to determine if chiral ice crystals could form under the conditions of interstellar molecular clouds and young stellar objects (protoplanetary disks) and to clarify the crystalline structure of these ices. Our results suggest that the following ice crystals are chiral: crystalline CO (α-CO) formed on amorphous H2O (a-H2O) grains in a 10-K molecular cloud, crystalline CH3OH formed by the heating of amorphous CH3OH on a-H2O grains at 40–60 K in young stellar objects, and several polymorphs of hydrogen-ordered cubic ice crystals formed by the heating of a-H2O at 80–100 K and direct condensation at 120–140 K in protoplanetary disks. We also investigated candidates for other chiral ices using published data. We found that NH3 I and NH3·H2O I are chiral at low temperature and pressure conditions. If one-handed circularly polarized light is irradiated during the nucleation of these chiral ice crystals, homochiral crystals can be formed. These results have important implications for the origin of interstellar organic molecule homochirality. - Feasibility of control of particle assembly by dielectrophoresis in liquid-cell transmission electron microscopy.
Tomoya Yamazaki, Hiromasa Niinomi, Yuki Kimura
Microscopy (Oxford, England), 71, 4, 231, 237, 01 Aug. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, Liquid-cell transmission electron microscopy (LC-TEM) is a useful technique for observing phenomena in liquid samples with spatial and temporal resolutions similar to those of conventional transmission electron microscopy (TEM). This method is therefore expected to permit the visualization of phenomena previously inaccessible to conventional optical microscopy. However, dynamic processes such as nucleation are difficult to observe by this method because of difficulties in controlling the condition of the sample liquid in the observation area. To approach this problem, we focused on dielectrophoresis, in which electrodes are used to assemble particles, and we investigated the phenomena that occurred when an alternating-current signal was applied to an electrode in an existing liquid cell by using a phase-contrast optical microscope (PCM) and TEM. In PCM, we observed that colloidal particles in a solution were attracted to the electrodes to form assemblies, that the particles aligned along the electric field to form pearl chains and that the pearl chains accumulated to form colloidal crystals. However, these phenomena were not observed in the TEM study because of differences in the design of the relevant holders. The results of our study imply that the particle assembly by using dielectrophoretic forces in LC-TEM should be possible, but further studies, including electric device development, will be required to realize this in practice. - Possible embryos and precursors of crystalline nuclei of calcium carbonate observed by liquid-cell transmission electron microscopy.
Yuki Kimura, Hiroyasu Katsuno, Tomoya Yamazaki
Faraday discussions, 235, 81, 94, 14 Jul. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Several different building blocks or precursors play an important role in the early stages of the crystallization of calcium carbonate (CaCO3). Many studies have been conducted over a long period to elucidate the fundamental processes involved in this crystallization. Here, we report the role of an amorphous phase and embryo at the beginning of the nucleation of CaCO3 from solutions of relatively low supersaturation. Prenucleation crystals formed in amorphous calcium carbonate (ACC) at a significantly large formation rate of 2 × 1022 m-3 s-1, suggesting that a low interfacial energy exists between the ACC and crystals. Only one calcite crystal exceeded the size for a critical nucleus (∼104 molecules) in 150 pre-nucleation crystals. Each pre-nucleation crystal might consist of a different polymorph, and ACCs have a similar composition and structure. A particle-detection algorithm, used in conjunction with machine learning, suggested that an embryo with a characteristic structure exists in solution and might play a crucial role in nucleation. No similar embryonic structure could be observed immediately after the dissolution of pre-nucleation particles, implying that their dissolution process is not simply the reverse process of their growth. This method should provide a new approach to understanding nucleation processes. - Low- and High-Density Unknown Waters at Ice-Water Interfaces
Hiromasa Niinomi, Akira Kouch, Tetsuya Hama, Hiroki Nada, Tomoya Yamazaki, Yuki Kimura
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 13, 19, 4251, 4256, May 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal - Fast Improvement of TEM Images with Low-Dose Electrons by Deep Learning.
Hiroyasu Katsuno, Yuki Kimura, Tomoya Yamazaki, Ichigaku Takigawa
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 28, 1, 138, 144, Feb. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Low electron dose observation is indispensable for observing various samples using a transmission electron microscope; consequently, image processing has been used to improve transmission electron microscopy (TEM) images. To apply such image processing to in situ observations, we here apply a convolutional neural network to TEM imaging. Using a dataset that includes short-exposure images and long-exposure images, we develop a pipeline for processed short-exposure images, based on end-to-end training. The quality of images acquired with a total dose of approximately $5$$e^{-}$ per pixel becomes comparable to that of images acquired with a total dose of approximately $1{,}000$$e^{-}$ per pixel. Because the conversion time is approximately 8 ms, in situ observation at 125 fps is possible. This imaging technique enables in situ observation of electron-beam-sensitive specimens. - Liquid-Cell Transmission Electron Microscopy Observation of Two-Step Collapse Dynamics of Silicon Nanopillars on Evaporation of Propan-2-ol: Implications for Semiconductor Integration Density
Sasaki, Y., Yamazaki, T., Kimura, Y.
ACS Applied Nano Materials, 5, 7, 2022, [Peer-reviewed]
Scientific journal - 極低電子線観察を可能にする深層学習によるTEM画像の鮮明化—Improvement of ultra-low dose electron image of TEM by deep learning—特集 機械学習・AIは結晶成長研究をいかに変えるか?
勝野 弘康, 木村 勇気, 山﨑 智也, 瀧川 一学
日本結晶成長学会誌 Journal of the Japanese Association for Crystal Growth, 49, 1, 1, 8, 2022, [Peer-reviewed], [Invited]
Japanese - Early Detection of Nucleation Events From Solution in LC-TEM by Machine Learning.
Hiroyasu Katsuno, Yuki Kimura, Tomoya Yamazaki, Ichigaku Takigawa
Frontiers in chemistry, 10, 818230, 818230, 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, To support the detection, recording, and analysis of nucleation events during in situ observations, we developed an early detection system for nucleation events observed using a liquid-cell transmission electron microscope. Detectability was achieved using the machine learning equivalent of detection by humans watching a video numerous times. The detection system was applied to the nucleation of sodium chloride crystals from a saturated acetone solution of sodium chlorate. Nanoparticles with a radius of more greater than 150 nm were detected in a viewing area of 12 μm × 12 μm by the detection system. The analysis of the change in the size of the growing particles as a function of time suggested that the crystal phase of the particles with a radius smaller than 400 nm differed from that of the crystals larger than 400 nm. Moreover, the use of machine learning enabled the detection of numerous nanometer sized nuclei. The nucleation rate estimated from the machine-learning-based detection was of the same order as that estimated from the detection using manual procedures. - UV-Induced Formation of Ice XI Observed Using an Ultra-High Vacuum Cryogenic Transmission Electron Microscope and its Implications for Planetary Science
Akira Kouchi, Yuki Kimura, Kensei Kitajima, Hiroyasu Katsuno, Hiroshi Hidaka, Yasuhiro Oba, Masashi Tsuge, Tomoya Yamazaki, Kazuyuki Fujita, Tetsuya Hama, Yukihiro Takahashi, Shunichi Nakatsubo, Naoki Watanabe
Frontiers in Chemistry, 9, 799851, 799851, Frontiers Media SA, 08 Dec. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, The occurrence of hydrogen atom-ordered form of ice Ih, ice XI, in the outer Solar System has been discussed based on laboratory experiments because its ferroelectricity influences the physical processes in the outer Solar System. However, the formation of ice XI in that region is still unknown due to a lack of formation conditions at temperatures higher than 72 K and the effect of UV-rays on the phase transition from ice I to ice XI. As a result, we observed the UV-irradiation process on ice Ih and ice Ic using a newly developed ultra-high vacuum cryogenic transmission electron microscope. We found that ice Ih transformed to ice XI at temperatures between 75 and 140 K with a relatively small UV dose. Although ice Ic partially transformed to ice XI at 83 K, the rate of transformation was slower than for ice Ih. These findings point to the formation of ice XI at temperatures greater than 72 K via UV irradiation of ice I crystals in the Solar System; icy grains and the surfaces of icy satellites in the Jovian and Saturnian regions. - Transmission Electron Microscopy Study of the Morphology of Ices Composed of H2O, CO2, and CO on Refractory Grains
Akira Kouchi, Masashi Tsuge, Tetsuya Hama, Yasuhiro Oba, Satoshi Okuzumi, Sin-iti Sirono, Munetake Momose, Naoki Nakatani, Kenji Furuya, Takashi Shimonishi, Tomoya Yamazaki, Hiroshi Hidaka, Yuki Kimura, Ken-ichiro Murata, Kazuyuki Fujita, Shunichi Nakatsubo, Shogo Tachibana, Naoki Watanabe
ASTROPHYSICAL JOURNAL, 918, 2, Sep. 2021, [Peer-reviewed]
English, Scientific journal - Radiolysis-Induced Crystallization of Sodium Chloride in Acetone by Electron Beam Irradiation
Tomoya Yamazaki, Yuki Kimura
MICROSCOPY AND MICROANALYSIS, 27, 3, 459, 465, Jun. 2021, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal - Rapid Ortho-to-para Nuclear Spin Conversion of H2 on a Silicate Dust Surface
M. Tsuge, T. Namiyoshi, K. Furuya, T. Yamazaki, A. Kouchi, N. Watanabe
The Astrophysical Journal, 908, 2, 234, 234, American Astronomical Society, 01 Feb. 2021, [Peer-reviewed]
Scientific journal - In-situ water-immersion experiments on amorphous silicates in the MgO-SiO2 system: implications for the onset of aqueous alteration in primitive meteorites
Yohei Igami, Akira Tsuchiyama, Tomoya Yamazaki, Megumi Matsumoto, Yuki Kimura
GEOCHIMICA ET COSMOCHIMICA ACTA, 293, 86, 102, Jan. 2021, [Peer-reviewed]
English, Scientific journal - Time-Resolved Observation of Evolution of Amyloid-β Oligomer with Temporary Salt Crystals
Nakajima, K., Yamazaki, T., Kimura, Y., So, M., Goto, Y., Ogi, H.
Journal of Physical Chemistry Letters, 11, 15, 6176, 6184, 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal - High-Density Liquid Water at a Water-Ice Interface
Niinomi, H., Yamazaki, T., Nada, H., Hama, T., Kouchi, A., Okada, J.T., Nozawa, J., Uda, S., Kimura, Y.
Journal of Physical Chemistry Letters, 11, 16, 6779, 6784, American Chemical Society (ACS), 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal - High mobility of lattice molecules and defects during the early stage of protein crystallization
Yamazaki, T., Van Driessche, A.E.S., Kimura, Y.
Soft Matter, 16, 8, 1955, 1960, Royal Society of Chemistry (RSC), 2020, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal - Immiscibility of Nucleating Aluminum Oxide Nanoparticles in Vapor
S. Ishizuka, Y. Kimura, J. Kawano, R. Escribano, T. Yamazaki, T. Hama, R. Sato
The Journal of Physical Chemistry C, 2018, 122, 25092, 25101, Oct. 2018, [Peer-reviewed]
English, Scientific journal - Sounding-rocket microgravity experiments on alumina dust
Shinnosuke Ishizuka, Yuki Kimura, Itsuki Sakon, Hiroshi Kimura, Tomoya Yamazaki, Shinsuke Takeuchi, Yuko Inatomi
Nature Communications, 9, 1, 3820, 3820, Sep. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Alumina (Al2O3) is believed to be the first major condensate to form in the gas outflow from oxygen-rich evolved stars because of the refractoriness and that α-Al2O3 (corundum, most stable polymorph) is a potential origin of a 13 μm feature that appears close to stars. However, no one has directly reproduced the 13 μm feature experimentally, and it has remained as a noteworthy unidentified infrared band. Here, we report nucleation experiments on Al2O3 nanoparticles monitored by a specially designed infrared spectrometer in the microgravity environment of a sounding rocket. The conditions approximate to those around asymptotic giant branch (AGB) stars. The measured spectra of the nucleated Al2O3 show a sharp feature at a wavelength of 13.55 μm and comparable in width to that observed near oxygen-rich AGB stars. Our finding that α-Al2O3 nucleates under certain condition provides a solid basis to elaborate condensation models of dust around oxygen-rich evolved stars. - Nucleation Processes of Cosmic Dust Investigated by Microgravity Experiments using an Airplane
Yuki Kimura, Shinnosuke Ishizuka, Sašo Šturm, Kyoko K. Tanaka, Tomoya Yamazaki, Fumiaki Saito, Yosuke Satoh, Katsuo Tsukamoto, Yuko Inatomi
International Journal of Microgravity Science and Application, 35, 3, 350305, Jul. 2018, [Peer-reviewed]
English, Scientific journal - Self-assembly of MoO3 needles in gas current for cubic formation pathway
Shinnosuke Ishizuka, Yuki Kimura, Satoki Yokoi, Tomoya Yamazaki, Rikako Sato, Tetsuya Hama
NANOSCALE, 9, 28, 10109, 10116, Jul. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal - Fast crystalline ice formation at extremely low temperature through water/neon matrix sublimation
Tetsuya Hama, Shinnosuke Ishizuka, Tomoya Yamazaki, Yuki Kimura, Akira Kouchi, Naoki Watanabe, Toshiki Sugimoto, Valerio Pirronello
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 19, 27, 17677, 17684, Jul. 2017, [Peer-reviewed]
English, Scientific journal - Two types of amorphous protein particles facilitate crystal nucleation
Tomoya Yamazaki, Yuki Kimura, Peter G. Vekilov, Erika Furukawa, Manabu Shirai, Hiroaki Matsumoto, Alexander E. S. Van Driessche, Katsuo Tsukamoto
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114, 9, 2154, 2159, Feb. 2017, [Peer-reviewed], [Lead author]
English, Scientific journal - Evolution analysis of V2O5.nH(2)O gels for preparation of xerogels having a high specific surface area and their replicas
Kanji Ishii, Yuki Kimura, Tomoya Yamazaki, Yuya Oaki, Hiroaki Imai
RSC ADVANCES, 7, 57, 35711, 35716, 2017, [Peer-reviewed]
English, Scientific journal - Two-Step Process in Homogeneous Nucleation of Alumina in Supersaturated Vapor
Shinnosuke Ishizuka, Yuki Kimura, Tomoya Yamazaki, Tetsuya Hama, Naoki Watanabe, Akira Kouchi
CHEMISTRY OF MATERIALS, 28, 23, 8732, 8741, Dec. 2016, [Peer-reviewed]
English, Scientific journal - In situ FT-IR study on the homogeneous nucleation of nanoparticles of titanium oxides from highly supersaturated vapor
Shinnosuke Ishizuka, Yuki Kimura, Tomoya Yamazaki
JOURNAL OF CRYSTAL GROWTH, 450, 168, 173, Sep. 2016, [Peer-reviewed]
English, Scientific journal - Assembling interferometers and in-situ observation of ambient environments and solid-liquid interfaces
Yuki Kimura, Kenta Murayama, Tomoya Yamazaki, Takao Maki
PROGRESS IN CRYSTAL GROWTH AND CHARACTERIZATION OF MATERIALS, 62, 2, 400, 403, Jun. 2016, [Peer-reviewed]
English, Scientific journal - Development of compartment for studies on the growth of protein crystals in space
T. Yamazaki, K. Tsukamoto, I. Yoshizaki, S. Fukuyama, H. Miura, T. Shimaoka, T. Maki, K. Oshi, Y. Kimura
REVIEW OF SCIENTIFIC INSTRUMENTS, 87, 3, 033107, Mar. 2016, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - 1D oriented attachment of calcite nanocrystals: formation of single-crystalline rods through collision
Mihiro Takasaki, Yuki Kimura, Tomoya Yamazaki, Yuya Oaki, Hiroaki Imai
RSC ADVANCES, 6, 66, 61346, 61350, 2016, [Peer-reviewed]
English, Scientific journal - Transmission Electron Microscopy of Crystallization of Lysozyme in a Solution
Tomoya Yamazaki, Manabu Shirai, Hiroaki Matsumoto, Yuki Kimura
Microscopy and Microanalysis, 21, S3, 255, 256, Cambridge University Press ({CUP}), Aug. 2015, [Lead author]
International conference proceedings - Correction of the equilibrium temperature caused by slight evaporation of water in protein crystal growth cells during long-term space experiments at International Space Station
Takahisa Fujiwara, Yoshihisa Suzuki, Izumi Yoshizaki, Katsuo Tsukamoto, Kenta Murayama, Seijiro Fukuyama, Kouhei Hosokawa, Kentaro Oshi, Daisuke Ito, Tomoya Yamazaki, Masaru Tachibana, Hitoshi Miura
REVIEW OF SCIENTIFIC INSTRUMENTS, 86, 8, 083704, Aug. 2015, [Peer-reviewed]
English, Scientific journal - Achiral Metastable Crystals of Sodium Chlorate Forming Prior to Chiral Crystals in Solution Growth
Hiromasa Niinomi, Tomoya Yamazaki, Shunta Harada, Toru Ujihara, Hitoshi Miura, Yuki Kimura, Takahiro Kuribayashi, Makio Uwaha, Katsuo Tsukamoto
CRYSTAL GROWTH & DESIGN, 13, 12, 5188, 5192, Dec. 2013, [Peer-reviewed]
English, Scientific journal - Growth rate measurements of lysozyme crystals under microgravity conditions by laser interferometry
Izumi Yoshizaki, Katsuo Tsukamoto, Tomoya Yamazaki, Kenta Murayama, Kentaro Oshi, Seijiro Fukuyama, Taro Shimaoka, Yoshihisa Suzuki, Masaru Tachibana
REVIEW OF SCIENTIFIC INSTRUMENTS, 84, 10, 103707, Oct. 2013, [Peer-reviewed]
English, Scientific journal - Crystal Growth of Hen Egg-White Lysozyme (HEWL) under Various Gravity Conditions
Weichun Pan, Jin Xu, Katsuo Tsukamoto, Masako Koizumi, Tomoya Yamazaki, Ru Zhou, Ang Li, Yuying Fu
Journal of Crystal Growth, 377, 43, 50, 2013, [Peer-reviewed]
English, Scientific journal
Other Activities and Achievements
- 航空機を用いた微小重力環境下における氷ナノ粒子の核生成実験の速報—Preliminary Results of Nucleation Experiments of Ice Nanoparticles in Microgravity Using an Aircraft
木村, 勇気, 山﨑, 智也, 屋嶋, 悠河, 中埜, 夕希, 森, 章一, 稲富, 裕光, KIMURA, Yuki, YAMAZAKI, Tomoya, YASHIMA, Yuga, NAKANO, Yuki, MORI, Shoichi, INATOMI, Yuko, 宇宙環境利用シンポジウム 第37回： 令和四年度 = Space Utilization Research, Vol. 37 2022: Proceedings of The Thirty-seventh Space Utilization Symposium, Jan. 2023
第37回宇宙環境利用シンポジウム (2023年1月17日-18日. オンライン開催)
Space Utilization Research (January 17-18, 2023. Online Meeting)
資料番号: SA6000180005
F-4, 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS), Japanese - Nucleation pathway by detection of nucleation of LC-TEM image using machine learning
勝野弘康, 木村勇気, 山崎智也, 瀧川一学, 瀧川一学, 日本物理学会講演概要集(CD-ROM), 77, 2, 2022 - Preparation for nucleation experiment of ice nanoparticles by the low-temperature gas evaporation method under microgravity condition
Kimura Yuki, Kouchi Akira, Yamazaki Tomoya, Satoh Yosuke, Saito Fumiaki, Inatomi Yuko, 宇宙環境利用シンポジウム 第34回： 令和元年度 = Space Utilization Research, Vol. 34 2019: Proceedings of The Thirty-fourth Space Utilization Symposium, 34, Jan. 2020
Space Utilization Research (January 21-22, 2020. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS)), Sagamihara, Kanagawa JapanE-04, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS), Japanese - 観測ロケットを用いた微小重力環境下でのダスト生成過程の再現実験
木村勇気, NUTH Joseph, BLUM Jurgen, 石塚紳之介, 田中今日子, 野沢貴也, 左近樹, 田中秀和, 木村宏, 山崎智也, 渡部直樹, 香内晃, STURM Saso, VELU Nirmal Kuma, 竹内伸介, 松原英雄, 稲富裕光, 日本惑星科学会秋季講演会予稿集(Web), 2018, 2018 - Elucidating the early processes of cosmic dust formation based on microgravity experiments using aircrafts
Kimura Yuki, Ishizuka Shinnosuke, Sturm Saso, Tanaka Kyoko K., Yamazaki Tomoya, Saito Fumiaki, Satoh Yosuke, Inatomi Yuko, 宇宙環境利用シンポジウム 第31回： 平成28年度 = Space Utilization Research, Vol. 31 2016: Proceedings of The Thirty-first Space Utilization Sysmposium, 31, Jan. 2017
Space Utilization Research (January 16-17, 2017. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS)), Sagamihara, Kanagawa Japan, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS), Japanese - Nucleation experiment of alumina and silica from vapor phase using the sounding rocket S-520-30
KIMURA Yuki, ISHIZUKA Shinnosuke, YAMAZAKI Tomoya, TANAKA Kyoko, TAKEUCHI Shinsuke, INATOMI Yuko, 日本地球惑星科学連合大会予稿集(Web), 2016, 2016 - In-situ IR measurement in homogeneous nucleation process of alumina under μG environment
ISHIZUKA Shinnosuke, KIMURA Yuki, YAMAZAKI Tomoya, SAKON Itsuki, INATOMI Yuko, 日本地球惑星科学連合大会予稿集(Web), 2016, 2016 - Microgravity experiments using S-520 sounding rockets to elucidate formation process of cosmic dust
Kimura Yuki, Ishizuka Shinnosuke, Nakatsubo Shunichi, Saito Fumiaki, Yamazaki Tomoya, Sakon Itsuki, Takeuchi Shinsuke, Inatomi Yuko, 第16回宇宙科学シンポジウム 講演集 = Proceedings of the 16th Space Science Symposium, Jan. 2016
16th Space Science Symposium (January 6-7, 2016. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS)Sagamihara Campus), Sagamihara, Kanagawa Japan, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS), Japanese - Reproduction experiment of cosmic dust particles using the Sounding rocket S-520-30
Kimura Yuki, Ishizuka Shinnosuke, Nakatsubo Shunichi, Saito Fumiaki, Yamazaki Tomoya, Sakon Itsuki, Takeuchi Shinsuke, Inatomi Yuko, 宇宙環境利用シンポジウム 第30回： 平成27年度 = Space Utilization Research, Vol. 30 2015: Proceedings of The Thirtieth Space Utilization Symposium, 30, Jan. 2016
Space Utilization Research (January 19-20, 2016. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS)), Sagamihara, Kanagawa Japan, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS), Japanese - Experimental results of sounding rocket S-520-30 and future plan
Kimura Yuki, Ishizuka Shinnosuke, Nakatsubo Shunichi, Sakon Itsuki, Takeuchi Shinsuke, Inatomi Yuko, 大気球シンポジウム: 平成27年度 = Balloon Symposium: 2015, Nov. 2015
Balloon Symposium 2015 (November 5-6, 2015. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa Japan, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS), Japanese
Lectures, oral presentations, etc.
- 湿度を制御した環境での透過電子顕微鏡法による結晶化その場観察の試み
山﨑智也, 木村勇気, 持田陸宏
日本顕微鏡学会大81回学術講演会, 10 Jun. 2025, Oral presentation - 環境セルを用いた透過電子顕微鏡法による 結晶化その場観察の試み
山崎智也
結晶表面・界面での相転移ダイナミクスに関する理論とその場観察の新展開, 20 Feb. 2025, Nominated symposium - 氷I相のナノ結晶における水素秩序相の形成
山崎智也, 香内晃, 村田憲一郎, 勝野弘康, 灘浩樹, 羽馬哲也, 木村勇気
JCCG-53, 18 Nov. 2024, Oral presentation - High internal pressure of the environmental cell indicated by the formation of clathrate hydrate
Tomoya Yamazaki, Tsutomu Uchida, Yuki Kimura
日本顕微鏡学会大80回学術講演会, 03 Jun. 2024, Japanese, Oral presentation - Transmission Electron Microscopic Observation of Clathrate Hydrates being Formed from Ice in High–Pressure Environments
Tomoya Yamazaki, Tsutomu Uchida, Yuki Kimura
JpGU 2024, 31 May 2024, Japanese, Oral presentation - 環境セルとペルチェ式冷却TEMホルダーを用いた氷、及び、クラスレートハイドレートの観察
山﨑智也, 内田努, 勝野弘康, 木村勇気
結晶表面での相転移ダイナミクスのその場観察と理論の新展開, Jan. 2024, Japanese, Invited oral presentation
[Invited] - Discovery of Clathrate Hydrate Crystals in Environmental-Cell Transmission Electron Microscopy
Tomoya Yamazaki, Tsutomu Uchida, Yuki Kimura
第52回結晶成長国内会議（JCCG-52）, Dec. 2023, Japanese, Oral presentation - In situ cryogenic transmission electron microscopy observation on the formation of hydrogen-ordered ices and its astrophysical implications
Tomoya Yamazaki, Akira Kouchi, Ken-Ichiro Murata, Hiroyasu Katsuno, Hiroki Nada, Tetsuya Hama, Yuki Kimura
Workshop on Interstellar Matter, Nov. 2023, English, Poster presentation - Ultra-High Vacuum Cryogenic Transmission Electron Microscopy of Ices Indicates the Possible formation of Hydrogen-Ordered Ices
Tomoya Yamazaki, Akira Kouchi, Ken-ichiro Murata, Hiroyasu Katsuno, Hiroki Nada, Tetsuya Hama, Yuki Kimura
15th International Conference on the Physics and Chemistry of Ice (PCI-2023), 05 Sep. 2023, English, Poster presentation - Liquid-Cell Transmission Electron Microscopy of ice crystals and bubble behaviors in the ice
Tomoya Yamazaki, Yuga Yashima, Hiroyasu Katsuno, Hiroya Miyazaki, Takashi Gondo, Yuki Kimura
15th International Conference on the Physics and Chemistry of Ice (PCI-2023), 05 Sep. 2023, English, Oral presentation - 環境セル中における電子ビーム照射によるクラスレートハイドレートの生成
山﨑智也, 木村勇気
日本顕微鏡学会第79回学術講演会, 27 Jun. 2023, Japanese, Poster presentation - 溶液セルとペルチェ式冷却TEMホルダーを用いた氷結晶のその場観察
山﨑智也, 屋嶋悠河, 勝野弘康, 宮崎裕也, 權堂貴志, 木村勇気
日本顕微鏡学会第79回学術講演会, 26 Jun. 2023, Japanese, Oral presentation - 溶液セル透過型電子顕微鏡法を用いた結晶化の研究
山﨑智也
令和５年度低温研セミナー第１回, 15 Jun. 2023, Japanese, Others - In situ observation of ice crystals using a Peltier cooling TEM holder and a liquid cell
Tomoya Yamazaki, Yuga Yashima, Hiroyasu Katsuno, Hiroya Miyazaki, Takashi Gondo, Yuki Kimura
日本地球惑星科学連合2023年大会, 21 May 2023, Japanese, Poster presentation - 環境セルを用いた新しい透過型電子顕微鏡法開発のための装置作製
山﨑智也, 新家寛正, 石塚紳之介, 森章一, 斎藤史明, 木村勇気
北海道大学低温科学研究所第28回技術報告会, 02 Dec. 2022, Japanese, Oral presentation - ペルチェ式冷却TEMホルダーと溶液セルを用いた結晶化その場観察
山﨑智也, 屋嶋悠河, 宮崎裕也, 權堂貴志, 木村勇気
第51回結晶成長国内会議（JCCG-51）, 31 Oct. 2022, Japanese, Oral presentation - Is dielectrophoresis effective for increasing local concentration of particles in liquid-cell transmission electron microscopy?
Tomoya Yamazaki, Hiromasa Niinomi, Hiroyasu Katsuno, Hooman Hosseinkhannazer, Eric Daigle, Yuki Kimura
Microscopy and Microanalysis 2022 (M&M 2022), 01 Aug. 2022, English, Oral presentation - Control of particles by electric field and its applicability in LC-TEM for observing crystallization and dissolution processes
Tomoya Yamazaki, Hiromasa Niinomi, Yuki Kimura
日本地球惑星科学連合2021年大会, 05 Jun. 2022, Japanese, Oral presentation - Dielectrophoresis of sub-micron-sized particles in liquid-cell transmission electron microscopy
Tomoya Yamazaki, Hiromasa Niinomi, Hiroyasu Katsuno, Yuki Kimura
日本地球惑星科学連合2022年大会, 22 May 2022, Japanese, Oral presentation - 誘電泳動による粒子挙動の溶液セルTEMその場観察
山﨑智也, 新家寛正, 勝野弘康, 木村勇気
日本顕微鏡学会第78回学術講演会, 13 May 2022, Japanese, Oral presentation - Supersaturation control for observation of crystallization in liquid-cell transmission electron microscopy
Tomoya Yamazaki, Yuki Kimura
The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021), 18 Dec. 2021, English, Oral presentation - 環境セル透過型電子顕微鏡法とその応用
山﨑智也, 石塚紳之介, 新家寛正, 木村勇気
連携型博士研究人材総合育成システム 次世代研究者シンポジウム, 27 Oct. 2021, Japanese, Poster presentation - 溶液セルTEMにおける結晶化条件制御方法の検討
山﨑智也, 木村勇気
日本顕微鏡学会第77回学術講演会, 16 Jun. 2021, Japanese, Oral presentation - Radiolysis-induced crystallization process of salt in liquid cell transmission electron microscopy
Tomoya Yamazaki, Yuki Kimura
日本地球惑星科学連合2020年大会, 27 May 2020, Japanese, Poster presentation - Electron-beam-induced nucleation in an antisolvent
Tomoya Yamazaki, Yuki Kimura
Microscopy & Microanalysis 2019 (M&M 2019), 06 Aug. 2019, English, Poster presentation - In situ observation of crystallization of a salt using an antisolvent by transmission electron microscopy
Tomoya Yamazaki, Yuki Kimura
International Conference on Crystal Growth and Epitaxy (ICCGE–19), 01 Aug. 2019, English - 溶液透過型電子顕微鏡を用いた貧溶媒からの結晶化その場観察法の検討
山﨑智也, 木村勇気
日本顕微鏡学会第75回学術講演会, 19 Jun. 2019, Japanese, Oral presentation - Investigation of liquid cell transmission electron microscopy combined with anti-solvent crystallization
Tomoya Yamazaki, Yuki Kimura
日本地球惑星科学連合2019年大会, 28 May 2019, Japanese, Oral presentation - 透過型電子顕微鏡による溶液からの結晶化“その場”観察法の検討
山﨑智也
結晶表面・界面での相転移ダイナミクスに関するその場観察および理論, 29 Jan. 2019, Japanese, Oral presentation - タンパク質結晶中の欠陥の挙動
山﨑智也, Alexander E. S. Van Driessche, 木村勇気
第47回結晶成長国内会議, 30 Oct. 2018, Japanese, Oral presentation - タンパク質結晶中の欠陥の動的観察
山﨑智也, 木村勇気
日本地球惑星科学連合2018年大会, 02 Jun. 2018, Japanese, Oral presentation - リゾチーム結晶の核生成経路
山﨑智也, 木村勇気, Alexander E. S. Van Driessche
結晶表面・界面での成長素過程のその場観察と理論, 26 Jan. 2018, Japanese, Oral presentation - 透過型電子顕微鏡を用いたリゾチーム結晶欠陥“その場”観察
山﨑智也, 木村勇気
第46回結晶成長国内会議, 29 Nov. 2017, Japanese, Oral presentation - In-situ observation of nucleation processes in solution by TEM
Yuki Kimura, Tomoya Yamazaki
13th Multinational Congress on Microscopy, 25 Sep. 2017, English, Invited oral presentation
[Invited] - 溶液透過型電子顕微鏡を用いたリゾチームタンパク質の結晶化その場観察
山﨑智也, 木村勇気
第73回日本顕微鏡学会学術講演会, 01 Jun. 2017, Japanese, Oral presentation - 溶液透過型電子顕微鏡によるリゾチームタンパク質の結晶化初期過程の動力学的研究
山﨑智也, 木村勇気
日本地球惑星科学連合2017年大会, May 2017, Japanese, Oral presentation - フルイド反応TEMを用いたタンパク質結晶化過程1分子“その場”観察
山﨑智也
平成28年度笹川科学研究奨励賞受賞研究発表会, 21 Apr. 2017, Japanese, Invited oral presentation
[Invited] - 溶液透過型電子顕微法が明らかにするリゾチームタンパク質の結晶化
山﨑智也, 木村勇気
結晶から見る地球科学～新鉱物の発見、人工結晶から初期生命まで～, 07 Mar. 2017, Japanese, Invited oral presentation
[Invited] - In-situ observation techniques for studying the protein crystal growth in space
Tomoya Yamazaki, Katsuo Tsukamoto, Izumi Yoshizaki, Seijiro Fukuyama, Hitoshi Miura, Taro Shimaoka, Takao Maki, Kentaro Oshi, Yuki Kimura
The 18th International Conference on Crystal Growth and Epitaxy, Aug. 2016, English, Poster presentation
08 Aug. 2016 - 12 Aug. 2016 - In-situ transmission electron microscopy of protein crystallization
Tomoya Yamazaki, Yuki Kimura
The 16th International Summer School on Crystal Growth, Aug. 2016, English, Poster presentation
01 Aug. 2016 - 07 Aug. 2016 - 水溶液からのタンパク質の結晶化のナノスケール“その場”観察
山﨑智也
低温物質の界面・ナノ現象ワークショップ2016, 13 Jul. 2016, Japanese, Nominated symposium - 透過型電子顕微鏡を用いたタンパク質の結晶化における準安定相の直接観察
山﨑智也, 木村勇気
日本地球惑星科学連合2016年大会, May 2016, Japanese, Oral presentation - 透過型電子顕微鏡を用いたリゾチーム結晶成長“その場”観察
山﨑智也, 木村勇気
第45回結晶成長国内会議, 19 Oct. 2015, Japanese, Poster presentation - In-situ observation of protein crystallization
Tomoya Yamazaki
The Slovene-Japan bilateral meeting, Sep. 2015, English, Oral presentation
30 Sep. 2015 - 02 Oct. 2015 - 透過型電子顕微鏡によるタンパク質結晶化“その場”観察
山﨑智也, 木村勇気
第39回結晶成長討論会, Sep. 2015, Japanese, Poster presentation - Transmission Electron Microscopy of Crystallization of Lysozyme in a Solution
T. Yamazaki, M. Shirai, H. Matsumoto, Y. Kimura
Microscopy and Microanalysis 2015, Aug. 2015, English, Poster presentation
02 Aug. 2015 - 06 Aug. 2015 - 溶液中の結晶化過程の研究に対する透過型電子顕微鏡の応用
山﨑智也, 木村勇気
日本地球惑星科学連合2015年大会, May 2015, Japanese, Oral presentation - フルイド反応TEMを用いた炭酸カルシウム核生成過程のTEM中“その場”観察
山﨑智也, 古川えりか, 木村勇気
日本地球惑星科学連合2014年大会, Apr. 2014, Japanese, Poster presentation - 14th International Conference on the Crystallization of Biological Macromolecules
K. Tsukamoto, K. Oshi, T. Yamazaki, H. Miura, I. Yoshizaki, S. Fukuyama, T. Shimaoka, T. Maki
Live Measurement of Growth Rate of Lysozyme Crystals in ISS, Sep. 2012, English, Invited oral presentation
23 Sep. 2012 - 28 Sep. 2012, [Invited] - JEM内での溶液からの結晶成長"その場"観察用セル
山﨑智也, 三浦均, 木村勇気, 吉崎泉, 福山誠二郎, 真木孝雄, 塚本勝男
第41回結晶成長国内会議, Nov. 2011, Japanese, Oral presentation - The study of the lysozyme crystal growth mechanisms using microgravity condition
Tomoya Yamazaki
ELyT School in Lyon, Sep. 2011, English, Oral presentation
03 Sep. 2011 - 15 Sep. 2011 - Lysozyme Crystal Growth Rate Increase under Microgravity Condition
T. Yamazaki, W. Pan, H. Miura, Y. Kimura, A. Srivastava, I. Yoshizaki, T. Maki, K. Tsukamoto
International Conference on Materials for Advanced Technologies, Jun. 2011, English, Poster presentation
26 Jun. 2011 - 01 Jul. 2011 - 宇宙実験に向けた干渉法によるリゾチーム結晶成長速度測定
山﨑智也, 三浦均, 木村勇気, 吉崎泉, 真木孝雄, 塚本勝男
日本地球惑星科学連合2011年大会, May 2011, Japanese, Oral presentation - Lysozyme Crystal Growth Rate Increase under Microgravity
T. Yamazaki, W. Pan, H. Miura, Y. Kimura, A. Srivastava, I. Yoshizaki, T. Maki, K. Tsukamoto
8th Japan-China-Korea Workshop on Microgravity Sciences, Sep. 2010, English, Oral presentation
22 Sep. 2010 - 24 Sep. 2010 - Lysozyme Crystal Growth Rate Increase under Microgravity
T. Yamazaki, W. Pan, H. Miura, Y. Kimura, A. Srivastava, I. Yoshizaki, T. Maki, K. Tsukamoto
The 14th International Conference on Crystal Growth, Aug. 2010, English, Invited oral presentation
08 Aug. 2010 - 13 Aug. 2010, [Invited] - 微小重力下でのリゾチーム結晶成長に対する不純物効果
山﨑智也, Pan Weichun, 三浦均, 木村勇気, 吉崎泉, 真木孝雄, 塚本勝男
日本地球惑星科学連合2010年大会, May 2010, Japanese, Oral presentation - 宇宙で結晶をつくる！
山﨑智也, Pan Weichun, 三浦均, Li Chaorong, 吉崎泉, 塚本勝男
第3回東北大学大学院理学研究科６専攻合同シンポジウム「ヤングブレインズの連帯による学際的研究の創造」, 16 Feb. 2010, Japanese, Oral presentation - 微小重力下でのリゾチーム結晶成長速度
山﨑智也, Pan Weichun, 三浦均, Li Chaorong, 吉崎泉, 塚本勝男
第39回結晶成長国内会議, Nov. 2009, Japanese, Poster presentation - 航空機実験におけるリゾチーム結晶のステップ成長速度変化
山﨑智也, Pan Weichun, 塚本勝男, Li Chaorong
第34回結晶成長討論会, Sep. 2009, Japanese, Poster presentation - Crystal Growth Mechanism: Influence of Impurity and Hydrodynamics
T. Yamazaki, K. Tsukamoto, W. Pan, C. Li, P. Dold, E. Ono, I. Yoshizaki, J. M. Garcia-Ruiz
The International School of Crystallization. 2009: Drugs, Foods and Agrochemicals, May 2009, English, Poster presentation
25 May 2009 - 29 May 2009 - Growth rate measurement of lysozyme crystals in microgravity
T. Yamazaki, W. Pan, C. Li, K. Tsukamoto, P. Dold, E. Ono, I. Yoshizaki, J. M. Garcia-Ruiz
The 2nd International Symposium “Interface Mineralogy” in conjunction with The 2nd International workshop "Crystallization in The Early Solar Nebula 4.6 Billion Years Ago", Mar. 2009, English, Poster presentation
09 Mar. 2009 - 12 Mar. 2009 - 微小重力下でのリゾチーム結晶成長実験に向けた種結晶の保存
山﨑智也, 塚本勝男, 吉崎泉
第38回結晶成長国内会議, Nov. 2008, Japanese, Oral presentation - 微小重力実験に向けたリゾチーム結晶の核形成制御
山﨑智也, 小野えりか, 塚本勝男
第32回結晶成長討論会, Nov. 2007, Japanese, Poster presentation
Research Themes
- 生体ライブTEMイメージング法の開発による微生物のナノ粒子合成過程の可視化
科学研究費助成事業
Apr. 2025 - Mar. 2029
木村 勇気, 山崎 智也
日本学術振興会, 基盤研究(A), 北海道大学, Coinvestigator, 25H00825 - 生物による炭酸塩形成プロセスの素過程への分解
科学研究費助成事業
Apr. 2025 - Mar. 2028
奥村 大河, 川野 潤, 荒木 優希, 山崎 智也
日本学術振興会, 学術変革領域研究(B), 早稲田大学, Coinvestigator, 25H01448 - Visualization of the formation process of clathrate hydrates by environmental-cell transmission electron microscopy
Grants-in-Aid for Scientific Research
Apr. 2025 - Mar. 2028
山崎 智也
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, 25K01676 - Exploring new indicators representing the hygroscopicity of atmospheric organic aerosol
Grants-in-Aid for Scientific Research
Apr. 2023 - Mar. 2027
持田 陸宏, 佐藤 圭, 木村 勇気, 松井 仁志, 山崎 智也, 宮崎 雄三, 大畑 祥, 石塚 紳之介
ソフトイオン化質量分析に基づいて大気エアロゾル試料に含まれる有機物の分子量分布を解析する手法について検討を開始した。具体的には、有機化合物の試薬と大気エアロゾル試料を用いて以下の予備的検討を行った。ポリエチレングリコール（average Mn 300, 400）の水溶液をシリンジに取り、シリンジポンプを用いてエレクトロスプレーイオン化質量分析計（ESI-MS）に水溶液を直接導入して質量スペクトルを取得した。そして、得られたスペクトルの信号から水溶液中のポリエチレングリコールの平均分子量を推定した。また、単一の有機化合物（レボグルコサン、フタル酸、コハク酸ほかの試薬）の水溶液や、複数の有機化合物（コハク酸、グルタル酸ほかを含む）の混合水溶液をESI-MSに導入して質量スペクトルを取得し、スペクトルのピークに対応するイオンを推定した。そのほか、名古屋大学で採取された大気エアロゾル試料に含まれる水溶性成分の抽出物、また同抽出物とコハク酸等の有機化合物試薬を混合した水溶液試料に対して、ESI-MSへの直接導入による質量スペクトルの測定を試行した。この水溶性成分の抽出物に対して、溶媒をメタノールに置換した試料に対するESI-MS測定も行い、水・メタノールのそれぞれを溶媒とする試料に対して平均分子量の推定を行った。
そのほか、大気エアロゾル試料から抽出したフミン様物質、硫酸アンモニウム、さらにそれらの混合物からなる微粒子の吸湿成長に関する論文の作成などにも取り組んだ。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Nagoya University, Coinvestigator, 23H00515 - Verification of feasibility of high temperature and high pressure environment in liquid-cell transmission electron microscopy
Grants-in-Aid for Scientific Research
09 Jul. 2021 - 31 Mar. 2024
山崎 智也
本研究では、透過型電子顕微鏡を用いて高温高圧環境における溶液試料の観察が可能かどうかを検証することを目的とする。溶液セル透過型電子顕微法は、電子ビームを透過できる薄膜を観察窓として有する溶液セルを用いて、溶液試料を透過型電子顕微鏡で観察する手法である。本手法において、グラフェンを薄膜として用いた場合、試料の厚みに応じて溶液試料に高い圧力がかかることが知られている。一方、試料の厚みを制御した溶液セルの作製は非常に困難である。本年度は厚みを制御しつつ、容易に作製が可能な溶液セルの開発に取り組んだ。まず、厚みの制御が可能な溶液セルの設計を行った。シリコン基板をベースとして、窒化ケイ素薄膜を観察窓として設置する。その観察窓に厚みを制御した井戸型の未貫通穴を設置した。この未貫通穴に溶液を充填させ、グラフェンで封をすることで溶液セルを作製する予定である。また観察では、できるだけ観察できる範囲は広いほうが良い。一方、どのくらいの穴のサイズまでグラフェンで封をすることができるかは不明である。そのため、穴のサイズを複数種類設置することでその検証を行えるようにした。また、密閉した溶液試料へは試料加熱TEMホルダーからの熱を効率よく伝えなければならない。これは、試料観察の妨げにならないような窒化ケイ素薄膜上に金属薄膜を設置することで、試料溶液への熱伝導を良くした。このように設計した溶液セルのベースとなるシリコンチップを作製した。
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, 21K18901 - Establishment of technique for supersaturation control in liquid cell transmission electron microscopy and its application to protein crystallization
Grants-in-Aid for Scientific Research
01 Apr. 2020 - 31 Mar. 2023
山崎 智也
本研究は溶液セルを用いた透過型電子顕微鏡法（TEM）において、過飽和度を制御する手法を開発し、溶液からの結晶の核生成を捉え、その描像を明らかにすることを目指す。特に、タンパク質の結晶化に着目し、その結晶核ができる瞬間を高い時間・空間分解能で可視化することを目指す。
昨年度は、既存の電極を搭載した溶液セルとタンパク質の結晶化系をスケールアップさせたコロイド粒子系を用い、コロイド粒子に誘電泳動力を働かせ、その挙動をその場観察した。その際、光学顕微鏡とは異なり、TEM観察においては粒子を電極の周りに効率よく集めることができないという現象を発見した。これは電極周囲に粒子を集めるために十分な誘電泳動力が粒子にかかっていないためである。またタンパク質などの数ナノメートルから数十ナノメートルの粒子に有効な誘電泳動力を働かせるには高い電場勾配力が必要である。これらを解決するため、本年度は電極間のギャップを数マイクロメートル以下にした溶液セルを作製した。これを用い、光学顕微鏡下でコロイド粒子、フェリチンタンパク質分子を観察したところ、電極間にこれらの粒子が集まる様子を捉えることに成功した。また、TEMによる観察では、コロイド粒子が電極周囲に集まっている様子を捉えることに成功した。集められたコロイド粒子はブラウン運動をしていたため、高濃度の液相として集合していると考えられる。また、電子ビームを強くすることによって、粒子の濃集体が発散する様子も捉えることに成功した。
また、試料冷却TEMホルダーを用いた液体試料の過飽和度制御は、既存の手法での溶液セル作製を検討したが、作製が非常に困難であった。一方、真空中で液体の状態を保持できるイオン液体をTEMグリッドに添付し、試料冷却TEMホルダーを用いて試料の冷却を行った。その結果、冷却によって結晶化したイオン液体の結晶を観察することに成功した。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 20H02580 - 透過型電子顕微鏡を用いた１分子トラッキングによるタンパク質の結晶化過程の解明
科学研究費助成事業
25 Apr. 2018 - 31 Mar. 2021
山崎 智也
溶液セルを用いた透過型電子顕微鏡法（TEM）は、結晶化の初期過程である核生成を明らかにできることが期待される一方、その瞬間を捉えることが非常に困難である。これは、既存の溶液セルを用いたTEMでは、観察視野内において結晶化を促進するように濃度などを変化させることが困難であるためである。これを解決するため、放射線分解と溶解度を極端に低下させる貧溶媒を併せた手法や電極を溶液セルに導入して結晶化を促進させる手法の検討を行った。
前者の手法では、塩素酸ナトリウムが飽和したアセトン溶液に電子ビームを照射すると、塩化ナトリウムが結晶化することが分かった。一方、塩素酸ナトリウムが飽和した水溶液に電子ビームを照射しても結晶化は観察されなかった。これらのことは、まず、塩素酸イオンがアセトンの放射線分解によって生成した還元剤により還元され、塩化物イオンが生成したことを示す。また、アセトンに対する塩化ナトリウムの溶解度が非常に低いため、すぐに塩化ナトリウムが溶け切れなくなって結晶化したと考えられる。一方、水の放射線分解ではアセトンよりも強力な還元剤が生成する。これは水溶液でも塩化物イオンが生成していることが期待され、観察視野で結晶化剤濃度を増加できることが期待できる。
後者の手法では、タンパク質分子を電極周囲に集めるには溶液セル自体を設計、作製しなければならないため、既存の電極が搭載されたチップを用いて溶液セルを作製し、コロイドを試料として、電場を印加したときの応答をその場観察した。電極周囲にコロイド粒子が集まり、それが結晶化する様子が観察された。このことは、今回用いた実験系をスケールダウンすることでタンパク質の結晶化に応用に期待できる。このため、タンパク質の結晶化用の溶液セルを設計し、作製に着手した。
日本学術振興会, 特別研究員奨励費, 北海道大学, 18J01470 - Evaluation of protein crystal defects in real and reciprocal space by in situ observation
Grants-in-Aid for Scientific Research
01 Apr. 2018 - 31 Mar. 2021
Yamazaki Tomoya
The dynamic behavior of defects in protein crystals, which degrade the quality of protein crystals, has been successfully visualized by transmission electron microscopy. The voids and the block structure were observed in protein crystals of micron size or smaller and their formation, movement, and disappearance were also observed. Analysis of these behaviors revealed that there is no time dependence on number density of defects in each crystal. This suggests that the defects originated before the crystals reached micron size, i.e., before, or immediately after nucleation.
Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, 18K14134 - In-situ observation of protein crystallization by fluid reaction-transmission electron microscopy (FR-TEM)
Grants-in-Aid for Scientific Research
29 Aug. 2014 - 31 Mar. 2016
YAMAZAKI Tomoya
The fluid reaction transmission electron microscopy (FR-TEM), which can directly observe inside liquids at nano-scale and in real time, was applied to crystallization of lysozyme protein in a solution.
By optimizing the crystallization condition, in-situ observation of crystallization of lysozyme was successfully performed. Two crystalline phases (orthorhombic and tetragonal) and an amorphous phase coexisted at nano-scale under the solution condition. The moment of the nucleation of orthorhombic lysozyme crystal was successfully captured.
Japan Society for the Promotion of Science, Grant-in-Aid for Research Activity Start-up, Hokkaido University, 26887001
