Nonoyama Takayuki

Faculty of Advanced Life Science　Advanced Transdisciplinary Science　Soft & Wet Matter ScienceAssociate Professor
Institute for the Advancement of Higher EducationAssociate Professor
Last Updated :2025/07/05

■Researcher basic information

Degree

  • Doctor of Engineering, Nagoya Institute of Technology, Mar. 2013

Profile Information

  • Takayuki NONOYAMA received his Doctor of Engineering degree from the Graduate School of Engineering, Nagoya Institute of Technology, in March 2013. He was appointed as a Specially Appointed Assistant Professor at the Laboratory of Soft & Wet Matter, Faculty of Advanced Life Science, Hokkaido University, in April 2013. He was promoted to Specially Appointed Associate Professor in April 2020, and has held a tenure-track Associate Professorship at the same faculty since June 2021. Since April 2025, he has also served as the Director of the Co-Creation Core for Soft Materials Aspiring Research & Translation, a new initiative at Hokkaido University aimed at advancing world-leading soft matter research through integration with medicine, engineering, agriculture, fisheries, and land–sea field sciences.


    He has also held cross-appointment positions at Hokkaido University, including:


    – the Global Station for Soft Matter, Global Institution for Collaborative Research and Education (GSS, GI-CoRE) from 2016 to 2020, and


    – the Department of High Toughness Hydrogels for Cartilage Application, Center for Innovation and Business Promotion from 2017 to 2021.
    Dr. NONOYAMA is actively engaged in the interdisciplinary field of polymers and bioceramics. His research has elucidated the mechanisms of bone formation and led to the development of materials incorporating bone-toughening strategies through materials science approaches. He has also contributed to the establishment of bone adhesion technologies. Notably, he discovered a class of soft materials that exhibit rapid and extreme hardening at elevated temperatures—behavior completely opposite to conventional polymers—advancing both fundamental understanding and applied developments in soft matter. More recently, he has been working on the development of real-space imaging techniques for polymer network structures by utilizing precision biomineralization technologies.

Researchmap personal page

Home Page URL

Researcher number

  • 50709251

ORCID ID

J-Global ID

Research Keyword

  • Glass Transition
  • Phase Separation
  • Soft ceramics
  • Metal nanoparticle
  • Self-assembled monolayer
  • Organic/Inorganic hybrid materials
  • Bone bonding
  • Hydroxyapatite
  • Polypeptide
  • Bioceramics
  • Hydrogel
  • Biomineralization

Research Field

  • Nanotechnology/Materials, Polymer materials
  • Nanotechnology/Materials, Inorganic materials
  • Natural sciences, Bio-, chemical, and soft-matter physics
  • Nanotechnology/Materials, Structural and functional materials
  • Nanotechnology/Materials, Composite materials and interfaces

Educational Organization

■Career

Career

  • Apr. 2025 - Present
    Co-Creation Core for Soft Materials Aspiring Research & Translation, Director, Japan
  • Jun. 2021 - Present
    Hokkaido University, Faculty of Advanced Life Science, Associate Professor
  • Apr. 2020 - May 2021
    Hokkaido University, Faculty of Advanced Life Science, Special-Appointment Associate Professor
  • Apr. 2020 - Mar. 2021
    Hokkaido University, Global Station for Soft Matter, Global Institution for Collaborative Research and Education (GSS, GI-CoRE), Special-Appointment Associate Professor
  • Jan. 2017 - Mar. 2021
    Center for Innovation and Business Promotion, Hokkaido University, Department of High Toughness Hydrogels for Cartilage Application
  • Apr. 2016 - Mar. 2020
    Global Station for Soft Matter, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Special-Appointment Assistant Professor
  • Apr. 2013 - Mar. 2020
    Hokkaido University, Faculty of Advanced Life Science, Special-Appointment Assistant Professor

Educational Background

  • Apr. 2010 - Mar. 2013, Nagoya Institute of Technology, Graduate School of Engineering, Department of Frontier Materials, Japan

■Research activity information

Awards

  • Jun. 2025, Japanese Society for Biomaterials, The Award for Young Investigator of Japanese Society for Biomaterials　　　　　　　　　　　　　　　
    Creation of Functional Biomaterials via Integration of Hydrogels and Bioceramics
  • Jun. 2022, Ceramics Society of Japan, The Ceramics Society of Japan (CerSJ) Awards for advancements in ceramic science and technology
    生体無機-高分子ゲル融合体による骨形成機序と骨再生誘導の研究
  • Sep. 2020, Ceramics Society of Japan, Presentation Award for Young Researcher in CSJ Fall Meeting　　　　　　　　　　　　　　　
    バイオミネラルをエネルギー散逸項とするハイドロゲルの強靭化
    Takayuki Nonoyama
  • May 2019, Society of Polymer Science, Japan, Award for Encouragement of Research in Polymer Science
    高靱性ゲル・バイオセラミックスの複合と骨形成誘導に関する研究
    nonoyamatakayuki
  • Sep. 2018, Society of Polymer Science, Japan, Publicity Award　　　　　　　　　　　　　　　
    高温で瞬時に1000倍以上硬くなる温度応答性アクティブソフトマテリアル
    nonoyamatakayuki
  • Sep. 2017, Ceramics Society of Japan, Presentation Award for Young Researchers　　　　　　　　　　　　　　　
    Interpenetrating Structure of Natural Bone Tissue & Synthesized Hydrogel
    nonoyamatakayuki, Japan society, Japan
  • Oct. 2014, International Union of Materials Research Societies, International Union of Materials Research Societies- International Conference in Asia, The Award for Encouragement of Research in IUMRS-ICA2014　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Mar. 2013, Nagoya Institute of Technology, President award in Nagoya Institute of Technology　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • May 2012, Ceramics Society of Japan, The best presentation award　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Mar. 2012, Nagoya Institute of Technology, President award in Nagoya Institute of Technology　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Dec. 2011, Tokai BO, Ceramics Society of Japan, The best presentation award　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Nov. 2011, Union of Chemistry-Related Societies in Chubu Area, Presentation award in Tokai BO of SPSJ　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Sep. 2011, Tokai BO, Society of Polymer Science, Japan, Presentation award　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Jul. 2011, Tokai BO, Ceramics Society of Japan, The best discussion award　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Jul. 2011, Tokai BO, Ceramics Society of Japan, Presentation award　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Mar. 2011, Nagoya Institute of Technology, Vice President award in Nagoya Institute of Technology　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Jan. 2011, Ceramics Society of Japan, World young fellow meeting 2011 Presentation award　　　　　　　　　　　　　　　
    nonoyamatakayuki
  • Jul. 2010, Tokai BO, Ceramics Society of Japan, The best presentation award　　　　　　　　　　　　　　　
    nonoyamatakayuki

Papers

  • Tendon Tissue Regeneration With Cell Orientation Using an Injectable Alginate-Cell Cross-linked Gel.
    Jun Yamaguchi, Kentaro Homan, Tomohiro Onodera, Masatake Matsuoka, Shoutaro Arakawa, Natsumi Ueda, Shiho Sawada, Nana Kawate, Takayuki Nonoyama, Yoshinori Katsuyama, Koji Nagahama, Mitsuru Saito, Norimasa Iwasaki
    The American journal of sports medicine, 3635465251325498, 3635465251325498, 23 Mar. 2025, [International Magazine]
    English, Scientific journal, BACKGROUND: Tendons have a limited blood supply and form inferior scar tissue during repair, which increases the risk of reruptures, causes complications, and limits regenerative capacity. Current methods to repair injured tendon tissue use solid scaffolds, which carry the risk of contamination (infections) and require open surgery for transplantation. HYPOTHESIS: Alginate-cell cross-linked gels, which can be applied by a percutaneous injection and transmit mechanical stress to cells via direct cell interaction, could induce tendon tissue regeneration. STUDY DESIGN: Controlled laboratory study. METHODS: A cross-linked gel was prepared to suspend azide-modified mesenchymal stromal cells (MSCs) in a dibenzocyclooctyne-modified branched alginic acid solution. The cross-linked gel was cultured in a bioreactor. In vivo, the Achilles tendon defects of 104 Lewis rats were injected with saline (control group), alginate gel alone (alginate group), alginate gel with MSCs (MSC group), and cross-linked gel (cross-link group). At 2 and 4 weeks postoperatively, histological and biochemical evaluations were performed. The biomechanical properties of repaired tissue were assessed at 4 weeks. RESULTS: In the bioreactor culture, the cell orientation in the cross-linked gel was parallel to the direction of tension. Histological analysis of the cross-link group showed significantly more repaired tendon tissue and improved collagen fiber orientation compared with the alginate group or MSC group. The biomechanical properties of the cross-link group included higher stiffness. CONCLUSION: The cross-linked gel was injectable at the injury site and was able to induce tissue regeneration with cell-oriented adaptability to the mechanical environment of tissue defects. CLINICAL RELEVANCE: Intercellular cross-linking technology holds the potential for clinical application as a minimally invasive therapeutic approach that can contribute to the qualitative improvement of tendon tissue regeneration.
  • Establishment of a novel method for differentiating into dopaminergic neurons using charged hydrogels.
    Bin Fan, Satoshi Tanikawa, Lei Wang, Takayuki Nonoyama, Yashitaka Oda, Zen-Ichi Tanei, Jian Ping Gong, Masumi Tsuda, Shinya Tanaka
    Biochemical and biophysical research communications, 747, 151280, 151280, 02 Feb. 2025, [International Magazine]
    English, Scientific journal, Parkinson's disease (PD) is a neurodegenerative disease primarily affecting the central nervous system and impacting both the motor system and non-motor systems. Although administration of L-DOPA is effective, it is not a fundamental treatment and has side effects such as diurnal fluctuation and dyskinesia, highlighting the need for new treatment methods. There is a growing interest in dopaminergic neuron transplantation as a potential treatment. Dopaminergic neurons derived from pluripotent stem (iPS) cells provide a valuable source for transplantation therapies. Developing an efficient method to differentiate iPS cells into dopaminergic cells is essential for cell transplantation therapy. While Cell differentiation is typically controlled by the addition of specific reagents, the physical characteristics of culture substrate, especially in the charge and stiffness, are also crucial factors in regulating differentiation. In this research, we show that two newly developed electrically charged polymeric hydrogels composed of cationic (C) and anionic (A) monomers inratio of 1-9 and 2 to 8 can significantly promote Dopaminergic neuron differentiation. Our findings emphasize the importance of culture substrates in effective dopaminergic cell differentiation.
  • Soft hydrogel-embedded ceramic skeleton mimicking bone structure via sacrificial bond concept.
    Sukamto, Miléna Lama, Jian Ping Gong, Takayuki Nonoyama
    Soft matter, 21, 2, 291, 303, 02 Jan. 2025, [International Magazine]
    English, Scientific journal, Bone, consisting of calcium phosphate minerals, rigid collagen fibrils, and acidic proteins, exhibits stiff and tough mechanical properties. On a molecular scale, covalent cross-linking in proteins and ionic interactions within proteins and at the protein-mineral boundary contribute to bone's toughness. In addition, hierarchical structures, like the sponge-like arrangement, are also crucial for the energy dissipation system in bone. Inspired by the multiple sacrificial bonds found in bone, we developed a soft/hard composite made up of two components with contrasting mechanical properties: a porous calcium phosphate skeleton and an acidic polymer hydrogel matrix. The porous ceramic skeleton alone is extremely rigid but brittle. However, the presence of the hydrogel matrix transforms the brittle nature of the porous ceramic skeleton into a soft/hard composite with stretchable and tough characteristics. The composite exhibits significant energy dissipation due to the fracture of the ceramic skeleton under small deformation, while catastrophic failure of the composite is prevented because the hybridized matrix disperses the damage throughout the entire sample. The Ca2+-mediated ionic bonding within the matrix hydrogel and at the boundary between the gel and skeleton effectively transfers the stress, enhancing the composite's toughness. Furthermore, the cyclic deformation generates new bare surfaces on the ceramic skeleton, leading to increased interaction between the matrix and these new surfaces, which enhances the composite's healing capability. This study demonstrates that the concept of multiple sacrificial bonds in bone is a smart strategy for designing polymer-ceramic composites with excellent mechanical properties.
  • Real-Space Visualization of Charged Polymer Network of Hydrogel by Double Network Strategy and Mineral Staining
    Shinji Noguchi, Ryuji Kiyama, Masahiro Yoshida, Maradhana Agung Marsudi, Naohiro Kashimura, Kiyoharu Tadanaga, Jian Ping Gong, Takayuki Nonoyama
    Nano Letters, American Chemical Society (ACS), 09 Jul. 2024, [Peer-reviewed], [Last author, Corresponding author]
    Scientific journal
  • 血管系IVRシミュレーションに適した3Dプリントによる透明柔軟な樹脂を用いた血管モデルの可能性　　　　　　　　　　　　　　　
    森田 亮, 野々山 貴行, 阿保 大介, 曽山 武士, 藤間 憲幸, 今井 哲秋, 高橋 文也, 木野田 直也, 加藤 大祐, 藤井 宝顕, 山崎 康之, 高柳 歩, 濱口 裕行, 亀田 拓人, 工藤 與亮
    日本インターベンショナルラジオロジー学会雑誌, 39, Suppl., 253, 253, (一社)日本インターベンショナルラジオロジー学会, Apr. 2024
    Japanese
  • Phase separation-induced glass transition under critical miscible conditions
    Mayu Watanabe, Dong Shi, Ryuji Kiyama, Kagari Maruyama, Yuichiro Nishizawa, Takayuki Uchihashi, Jian Ping Gong, Takayuki Nonoyama
    Materials Advances, Royal Society of Chemistry (RSC), 2024, [Peer-reviewed], [Last author, Corresponding author]
    Scientific journal, A polymer material shows phase separation-induced glass transition under critical miscible conditions, where the miscible/immiscible state is altered by temperature variations predicted by temperature-corrected Hansen solubility parameters.
  • Fractographic mirror law for brittle fracture of nonlinear elastic soft materials
    Ryuji Kiyama, Yong Zheng, Takayuki Nonoyama, Jian Ping Gong
    Soft Matter, 19, 40, 7724, 7730, Royal Society of Chemistry (RSC), Sep. 2023, [Peer-reviewed]
    English, Scientific journal, The first fractographic mirror radius analysis for soft hydrogel materials was conducted. The universal −1 power law between fracture stress and mirror radius was established, in contrast to the different power law of linear elastic hard materials.
  • Engineering of an electrically charged hydrogel implanted into a traumatic brain injury model for stepwise neuronal tissue reconstruction
    Satoshi Tanikawa, Yuki Ebisu, Tomáš Sedlačík, Shingo Semba, Takayuki Nonoyama, Takayuki Kurokawa, Akira Hirota, Taiga Takahashi, Kazushi Yamaguchi, Masamichi Imajo, Hinako Kato, Takuya Nishimura, Zen-ichi Tanei, Masumi Tsuda, Tomomi Nemoto, Jian Ping Gong, Shinya Tanaka
    Scientific Reports, 13, 1, Springer Science and Business Media LLC, 14 Feb. 2023, [Peer-reviewed]
    Scientific journal, Abstract

    Neural regeneration is extremely difficult to achieve. In traumatic brain injuries, the loss of brain parenchyma volume hinders neural regeneration. In this study, neuronal tissue engineering was performed by using electrically charged hydrogels composed of cationic and anionic monomers in a 1:1 ratio (C1A1 hydrogel), which served as an effective scaffold for the attachment of neural stem cells (NSCs). In the 3D environment of porous C1A1 hydrogels engineered by the cryogelation technique, NSCs differentiated into neuroglial cells. The C1A1 porous hydrogel was implanted into brain defects in a mouse traumatic damage model. The VEGF-immersed C1A1 porous hydrogel promoted host-derived vascular network formation together with the infiltration of macrophages/microglia and astrocytes into the gel. Furthermore, the stepwise transplantation of GFP-labeled NSCs supported differentiation towards glial and neuronal cells. Therefore, this two-step method for neural regeneration may become a new approach for therapeutic brain tissue reconstruction after brain damage in the future.
  • Tough Hydroxyapatite Hydrogels Based on Bone-like Self-Regulatory Sacrificial Bond Formation
    Naohiro Kashimura, Yuki Suzuki, Takayuki Nonoyama, Jian Ping Gong
    Chemistry of Materials, 2023, [Peer-reviewed], [Corresponding author]
    Scientific journal
  • Mechanical Properties of a 3 Dimensional–Printed Transparent Flexible Resin Used for Vascular Model Simulation Compared with Those of Porcine Arteries
    Ryo Morita, Takayuki Nonoyama, Daisuke Abo, Takeshi Soyama, Noriyuki Fujima, Tetsuaki Imai, Hiroyuki Hamaguchi, Takuto Kameda, Osamu Sugita, Bunya Takahashi, Naoya Kinota, Kohsuke Kudo
    Journal of Vascular and Interventional Radiology, Elsevier BV, Jan. 2023, [Peer-reviewed]
    Scientific journal
  • ERK MAP Kinase Signaling Regulates RAR Signaling to Confer Retinoid Resistance on Breast Cancer Cells
    Akira Hirota, Jean-Emmanuel Clément, Satoshi Tanikawa, Takayuki Nonoyama, Tamiki Komatsuzaki, Jian Ping Gong, Shinya Tanaka, Masamichi Imajo
    Cancers, 14, 23, 5890, 5890, MDPI AG, 29 Nov. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Retinoic acid (RA) and its synthetic derivatives, retinoids, have been established as promising anticancer agents based on their ability to regulate cell proliferation and survival. Clinical trials, however, have revealed that cancer cells often acquire resistance to retinoid therapy. Therefore, elucidation of underlying mechanisms of retinoid resistance has been considered key to developing more effective use of retinoids in cancer treatment. In this study, we show that constitutive activation of ERK MAP kinase signaling, which is often caused by oncogenic mutations in RAS or RAF genes, suppresses RA receptor (RAR) signaling in breast cancer cells. We show that activation of the ERK pathway suppresses, whereas its inhibition promotes, RA-induced transcriptional activation of RAR and the resultant upregulation of RAR-target genes in breast cancer cells. Importantly, ERK inhibition potentiates the tumor-suppressive activity of RA in breast cancer cells. Moreover, we also reveal that suppression of RAR signaling and activation of ERK signaling are associated with poor prognoses in breast cancer patients and represent hallmarks of specific subtypes of breast cancers, such as basal-like, HER2-enriched and luminal B. These results indicate that ERK-dependent suppression of RAR activity underlies retinoid resistance and is associated with cancer subtypes and patient prognosis in breast cancers.
  • Nanoscale TEM Imaging of Hydrogel Network Architecture
    Ryuji Kiyama, Masahiro Yoshida, Takayuki Nonoyama, Tomáš Sedlačík, Hiroshi Jinnai, Takayuki Kurokawa, Tasuku Nakajima, Jian Ping Gong
    Advanced Materials, 35, 1, 2208902, 2208902, Wiley, 09 Nov. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal, In this work, the authors succeed in direct visualization of the network structure of synthetic hydrogels with transmission electron microscopy (TEM) by developing a novel staining and network fixation method. Such a direct visualization is not carried out because sample preparation and obtaining sufficient contrast are challenging for these soft materials. TEM images reveal robust heterogeneous network architectures at mesh size scale and defects at micro-scale. TEM images also reveal the presence of abundant dangling chains on the surface of the hydrogel network. The real space structural information provides a comprehensive perspective that links bulk properties with a nanoscale network structure, including fracture, adhesion, sliding friction, and lubrication. The presented method has the potential to advance the field.
  • Force-triggered rapid microstructure growth on hydrogel surface for on-demand functions
    Mu, Q., Cui, K., Wang, Z.J., Matsuda, T., Cui, W., Kato, H., Namiki, S., Yamazaki, T., Frauenlob, M., Nonoyama, T., Tsuda, M., Tanaka, S., Nakajima, T., Gong, J.P.
    Nature Communications, 13, 1, Springer Science and Business Media LLC, 20 Oct. 2022, [Peer-reviewed]
    Scientific journal
  • Gluing blood into gel by electrostatic interaction using a water-soluble polymer as an embolic agent.
    Zhiping Jin, Hailong Fan, Toshiya Osanai, Takayuki Nonoyama, Takayuki Kurokawa, Hideki Hyodoh, Kotaro Matoba, Akiko Takeuchi, Jian Ping Gong, Miki Fujimura
    Proceedings of the National Academy of Sciences of the United States of America, 119, 42, e2206685119, 18 Oct. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Liquid embolic agents are widely used for the endovascular embolization of vascular conditions. However, embolization based on phase transition is limited by the adhesion of the microcatheter to the embolic agent, use of an organic solvent, unintentional catheter retention, and other complications. By mimicking thrombus formation, a water-soluble polymer that rapidly glues blood into a gel without triggering coagulation was developed. The polymer, which consists of cationic and aromatic residues with adjacent sequences, shows electrostatic adhesion with negatively charged blood substances in a physiological environment, while common polycations cannot. Aqueous polymer solutions are injectable through clinical microcatheters and needles. The formed blood gel neither adhered to the catheter nor blocked the port. Postoperative computed tomography imaging showed that the polymer can block the rat femoral artery in vivo and remain at the injection site without nontarget embolization. This study provides an alternative for the development of waterborne embolic agents.
  • Bioceramics x soft material as a simple model to mimic functions in bones
    Nonoyama, T.
    Journal of the Ceramic Society of Japan, 130, 10, 817, 824, Ceramic Society of Japan, 01 Oct. 2022, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
    Scientific journal
  • The mechanosensitive ion channel PIEZO1 is expressed in tendons and regulates physical performance.
    Ryo Nakamichi, Shang Ma, Takayuki Nonoyama, Tomoki Chiba, Ryota Kurimoto, Hiroki Ohzono, Merissa Olmer, Chisa Shukunami, Noriyuki Fuku, Guan Wang, Errol Morrison, Yannis P Pitsiladis, Toshifumi Ozaki, Darryl D'Lima, Martin Lotz, Ardem Patapoutian, Hiroshi Asahara
    Science translational medicine, 14, 647, eabj5557, Jun. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, How mechanical stress affects physical performance via tendons is not fully understood. Piezo1 is a mechanosensitive ion channel, and E756del PIEZO1 was recently found as a gain-of-function variant that is common in individuals of African descent. We generated tendon-specific knock-in mice using R2482H Piezo1, a mouse gain-of-function variant, and found that they had higher jumping abilities and faster running speeds than wild-type or muscle-specific knock-in mice. These phenotypes were associated with enhanced tendon anabolism via an increase in tendon-specific transcription factors, Mohawk and Scleraxis, but there was no evidence of changes in muscle. Biomechanical analysis showed that the tendons of R2482H Piezo1 mice were more compliant and stored more elastic energy, consistent with the enhancement of jumping ability. These phenotypes were replicated in mice with tendon-specific R2482H Piezo1 replacement after tendon maturation, indicating that PIEZO1 could be a target for promoting physical performance by enhancing function in mature tendon. The frequency of E756del PIEZO1 was higher in sprinters than in population-matched nonathletic controls in a small Jamaican cohort, suggesting a similar function in humans. Together, this human and mouse genetic and physiological evidence revealed a critical function of tendons in physical performance, which is tightly and robustly regulated by PIEZO1 in tenocytes.
  • Evaluation of biological responses to micro-particles derived from a double network hydrogel.
    Gen Matsumae, Mohamad Alaa Terkawi, Takayuki Nonoyama, Takayuki Kurokawa, Daisuke Takahashi, Tomohiro Shimizu, Ken Kadoya, Jian Ping Gong, Kazunori Yasuda, Norimasa Iwasaki
    Biomaterials science, 10, 9, 2182, 2187, 04 May 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Double network hydrogels (DN gels) composed of poly (2-acrylamido-2-methyl propanesulfonic acid) (PAMPS) as the brittle first network and poly (N,N-dimethylacrylamide) (PDMA) as the ductile second network have been proven to be a substitute biomaterial for cartilage, with promising biocompatibility and low toxicity, when they are used as bulk materials. For their further applications as articular cartilages, it is essential to understand the biological reactions and adverse events that might be initiated by wear particles derived from these materials. In this study, we used DN gel micro-particles of sizes 4 μm and 10 μm generated by the grinding method to mimic wearing debris of DN gels. The biological responses to particles were then evaluated in a macrophage-cultured system and an inflammatory osteolysis murine model. Our results demonstrated that DN gel particles have the ability to activate macrophages and promote the expression of Tnf-α, both in vitro and in vivo. Furthermore, the implantation of these particles onto calvarial bone triggered local inflammation and bone loss in a mouse model. Our data reveal that the potential foreign body responses to the generated particles from artificial cartilage should receive more attention in artificial cartilage engineering with the goal of developing a safer biocompatible substitute.
  • 細胞外基質の電位変化に伴うJCウイルス増殖の制御　　　　　　　　　　　　　　　
    谷川 聖, 野々山 貴行, 津田 真寿美, 王 磊, 種井 善一, Gong Jian Ping, 田中 伸哉
    日本病理学会会誌, 111, 1, 263, 263, (一社)日本病理学会, Mar. 2022
    Japanese
  • 細胞外基質の電位変化に伴うJCウイルス増殖の制御　　　　　　　　　　　　　　　
    谷川 聖, 野々山 貴行, 津田 真寿美, 王 磊, 種井 善一, Gong Jian Ping, 田中 伸哉
    日本病理学会会誌, 111, 1, 263, 263, (一社)日本病理学会, Mar. 2022
    Japanese
  • Combination of ultra-purified stem cells with an in situ-forming bioresorbable gel enhances intervertebral disc regeneration.
    Daisuke Ukeba, Katsuhisa Yamada, Takashi Suyama, Darren R Lebl, Takeru Tsujimoto, Takayuki Nonoyama, Hirokazu Sugino, Norimasa Iwasaki, Masatoki Watanabe, Yumi Matsuzaki, Hideki Sudo
    EBioMedicine, 76, 103845, 103845, 24 Jan. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, BACKGROUND: Lumbar intervertebral disc (IVD) herniations are associated with significant disability. Discectomy is the conventional treatment option for IVD herniations but causes a defect in the IVD, which has low self-repair ability, thereby representing a risk of further IVD degeneration. An acellular, bioresorbable, and good manufacturing practice (GMP)-compliant in situ-forming gel, which corrects discectomy-associated IVD defects and prevents further IVD degeneration had been developed. However, this acellular matrix-based strategy has certain limitations, particularly in elderly patients, whose tissues have low self-repair ability. The aim of this study was to investigate the therapeutic efficacy of using a combination of newly-developed, ultra-purified, GMP-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs) and the gel for IVD regeneration after discectomy in a sheep model of severe IVD degeneration. METHODS: RECs and nucleus pulposus cells (NPCs) were co-cultured in the gel. In addition, RECs combined with the gel were implanted into IVDs following discectomy in sheep with degenerated IVDs. FINDINGS: Gene expression of NPC markers, growth factors, and extracellular matrix increased significantly in the co-culture compared to that in each mono-culture. The REC and gel combination enhanced IVD regeneration after discectomy (up to 24 weeks) in the severe IVD degeneration sheep model. INTERPRETATION: These findings demonstrate the translational potential of the combination of RECs with an in situ-forming gel for the treatment of herniations in degenerative human IVDs. FUNDING: Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.
  • Hydroxyapatite-hybridized double-network hydrogel surface enhances differentiation of bone marrow-derived mesenchymal stem cells to osteogenic cells.
    Takuma Kaibara, Lei Wang, Masumi Tsuda, Takayuki Nonoyama, Takayuki Kurokawa, Norimasa Iwasaki, Jian Ping Gong, Shinya Tanaka, Kazunori Yasuda
    Journal of biomedical materials research. Part A, 110, 4, 747, 760, 28 Oct. 2021, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Recently, we have developed a hydroxyapatite (HAp)-hybridized double-network (DN) hydrogel (HAp/DN gel), which can robustly bond to the bone tissue in the living body. The purpose of this study is to clarify whether the HAp/DN gel surface can differentiate the bone marrow-derived mesenchymal stem cells (MSCs) to osteogenic cells. We used the MSCs which were harvested from the rabbit bone marrow and cultured on the polystyrene (PS) dish using the autogenous serum-supplemented medium. First, we confirmed the properties of MSCs by evaluating colony forming unit capacity, expression of MSC markers using flow cytometry, and multidifferential capacity. Secondly, polymerase chain reaction analysis demonstrated that the HAp/DN gel surface significantly enhanced mRNA expression of the eight osteogenic markers (TGF-β1, BMP-2, Runx2, Col-1, ALP, OPN, BSP, and OCN) in the cultured MSCs at 7 days than the PS surfaces (p < 0.0001), while the DN gel and HAp surfaces provided no or only a slight effect on the expression of these markers except for Runx2. Additionally, the alkaline phosphatase activity was significantly higher in the cells cultured on the HAp/DN gel surface than in the other three material surfaces (p < 0.0001). Thirdly, when the HAp/DN gel plug was implanted into the rabbit bone marrow, MSC marker-positive cells were recruited in the tissue generated around the plug at 3 days, and Runx2 and OCN were highly expressed in these cells. In conclusion, this study demonstrated that the HAp/DN gel surface can differentiate the MSCs into osteogenic cells.
  • Fast in vivo fixation of double network hydrogel to bone by monetite surface hybridization
    Takayuki Nonoyama, Lei Wang, Ryuji Kiyama, Naohiro Kashimura, Kazunori Yasuda, Shinya Tanaka, Takayuki Kurokawa, Jian Ping Gong
    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 129, 9, 584, 589, Sep. 2021, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • ハイドロキシアパタイト複合化ダブルネットワークゲル上での骨髄間葉系幹細胞の骨分化能評価
    甲斐原 拓真, 王 磊, 津田 真寿美, 野々山 貴行, 黒川 孝幸, きょう 剣萍, 岩崎 倫政, 田中 伸哉, 安田 和則
    日本整形外科学会雑誌, 95, 8, S1561, S1561, (公社)日本整形外科学会, Aug. 2021
    Japanese
  • Ultrapurified Alginate Gel Containing Bone Marrow Aspirate Concentrate Enhances Cartilage and Bone Regeneration on Osteochondral Defects in a Rabbit Model
    Liang Xu, Atsushi Urita, Tomohiro Onodera, Ryosuke Hishimura, Takayuki Nonoyama, Masanari Hamasaki, Dawei Liang, Kentaro Homan, Jian Ping Gong, Norimasa Iwasaki
    The American Journal of Sports Medicine, 036354652110141, 036354652110141, SAGE Publications, 01 Jun. 2021, [Peer-reviewed]
    Scientific journal, Background: Ultrapurified alginate (UPAL) gel implantation has been demonstrated as effective in cartilage repair for osteochondral defects; however, cell transplantation within UPAL gels would be required to treat larger defects.

    Hypothesis: The combination of UPAL gel and bone marrow aspirate concentrate (BMAC) would enhance cartilage repair and subchondral bone repair for large osteochondral defects.

    Study Design: Controlled laboratory study.

    Methods: A total of 104 osteochondral defects (1 defect per knee) of 52 rabbits were randomly divided into 4 groups (26 defects per group): defects without any treatment (Defect group), defects treated using UPAL gel alone (UPAL group), defects treated using UPAL gel containing allogenic bone marrow mesenchymal stromal cells (UPAL-MSC group), and defects treated using UPAL gel containing BMAC (UPAL-BMAC group). At 4 and 16 weeks postoperatively, macroscopic and histologic evaluations and measurements of repaired subchondral bone volumes of reparative tissues were performed. Collagen orientation and mechanical properties of the reparative tissue were assessed at 16 weeks.

    Results: The defects in the UPAL-BMAC group were repaired with hyaline-like cartilage with well-organized collagen structures. The histologic scores at 4 weeks were significantly higher in the UPAL-BMAC group (16.9 ± 2.0) than in the Defect group (4.7 ± 1.9; P < .05), the UPAL group (10.0 ± 3.3; P < .05), and the UPAL-MSC group (12.2 ± 2.9; P < .05). At 16 weeks, the score in the UPAL-BMAC group (24.4 ± 1.7) was significantly higher than those in the Defect group (9.0 ± 3.7; P < .05), the UPAL group (14.2 ± 3.9; P < .05), and the UPAL-MSC group (16.3 ± 3.6; P < .05). At 4 and 16 weeks, the macroscopic evaluations were significantly superior in the UPAL-BMAC group compared with the other groups, and the values of repaired subchondral bone volumes in the UPAL-BMAC group were significantly higher than those in the Defect and UPAL groups. The mechanical properties of the reparative tissues were significantly better in the UPAL-BMAC group than in the other groups.

    Conclusion: The implantation of UPAL gel containing BMAC-enhanced hyaline-like cartilage repair and subchondral bone repair of osteochondral defects in a rabbit knee model.

    Clinical Relevance: These data support the potential clinical application of 1-step treatment for large osteochondral defects using biomaterial implantation with cell transplantation.

  • Tough Double Network Hydrogel and Its Biomedical Applications.
    Takayuki Nonoyama, Jian Ping Gong
    Annual review of chemical and biomolecular engineering, 26 Mar. 2021, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal, Soft and wet hydrogels have many similarities to biological tissues, though their mechanical fragility had been one of the biggest obstacles in biomedical applications. Studies and developments in double network (DN) hydrogels have elucidated how to create tough gels universally based on sacrificial bond principles and opened a path for biomedical application of hydrogels in regenerative medicine and artificial soft connective tissues, such as cartilage, tendon, and ligament, which endure high tension and compression. This review explores a universal toughening mechanism for and biomedical studies of DN hydrogels. Moreover, because the term sacrificial bonds has been mentioned often in studies of bone tissues, consisting of biomacromolecules and biominerals, recent studies of gel-biomineral composites to understand early-stage osteogenesis and to simulate bony sacrificial bonds are also summarized. Expected final online publication date for the Annual Review of Chemical and Biomolecular Engineering, Volume 12 is June 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.
  • Bone Marrow Aspirate Concentrate Combined with in Situ Forming Bioresorbable Gel Enhances Intervertebral Disc Regeneration in Rabbits.
    Daisuke Ukeba, Katsuhisa Yamada, Takeru Tsujimoto, Katsuro Ura, Takayuki Nonoyama, Norimasa Iwasaki, Hideki Sudo
    The Journal of bone and joint surgery. American volume, 103, 8, e31, 21 Jan. 2021, [Peer-reviewed], [International Magazine]
    English, Scientific journal, BACKGROUND: The current surgical procedure of choice for intervertebral disc (IVD) herniation is discectomy, which induces postoperative IVD degeneration. Thus, cell-based therapies, as a 1-step simple procedure, are desired because of the poor capacity of IVDs for self-repair. The aim of this study was to investigate the repair efficacy of ultra-purified alginate (UPAL) gels containing bone marrow aspirate concentrate (BMAC) for the treatment of discectomy-associated IVD degeneration in rabbits. METHODS: The mechanical properties of 3 types of gels-UPAL, UPAL containing bone marrow-derived mesenchymal stem cells (BMSCs), and UPAL containing BMAC-were evaluated. Forty rabbits were assigned to 5 groups: intact control, discectomy (to make the cavity), UPAL (implantation of the UPAL gel after discectomy), BMSCs-UPAL (implantation of a combination of autogenic BMSCs and UPAL gel after discectomy), and BMAC-UPAL (implantation of a combination of BMAC and UPAL gel after discectomy). The gels were implanted at 4 weeks after induction of IVD degeneration. At 4 and 12 weeks, magnetic resonance imaging (MRI) as well as histological and immunohistochemical analyses were performed to analyze IVD degeneration qualitatively and the viability of the implanted cells. RESULTS: There was no significant difference among the 3 types of gels in terms of the results of unconfined compression tests. The implanted cells survived for 12 weeks. The histological grades of the BMSCs-UPAL (mean and standard deviation, 2.50 ± 0.53; p < 0.001) and BMAC-UPAL (2.75 ± 0.64, p = 0.001) showed them to be more effective in preventing degeneration than UPAL gel alone (3.63 ± 0.52). The effectiveness of BMAC-UPAL was not significantly different from that of BMSCs-UPAL, except with respect to type-II collagen synthesis. CONCLUSIONS: BMAC-UPAL significantly enhanced the repair of IVD defects created by discectomy. This approach could be an effective therapeutic strategy owing to its simplicity and cost-effectiveness compared with cell therapy using culture-expanded BMSCs. CLINICAL RELEVANCE: Local administration of the BMAC combined with UPAL gel could be an effective therapeutic strategy to enhance IVD repair after discectomy.
  • Isotope Microscopic Observation of Osteogenesis Process Forming Robust Bonding of Double Network Hydrogel to Bone.
    Takayuki Nonoyama, Lei Wang, Masumi Tsuda, Yuki Suzuki, Ryuji Kiyama, Kazunori Yasuda, Shinya Tanaka, Kousuke Nagata, Ryosuke Fujita, Naoya Sakamoto, Noriyuki Kawasaki, Hisayoshi Yurimoto, Jian Ping Gong
    Advanced healthcare materials, 10, 3, e2001731, 16 Nov. 2020, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
    English, Scientific journal, Tough double network (DN) hydrogels are promising substitutes of soft supporting tissues such as cartilage and ligaments. For such applications, it is indispensable to robustly fix the hydrogels to bones with medically feasible methods. Recently, robustly bonding the DN hydrogels to defected bones of rabbits in vivo has been proved successful. The low crystalline hydroxyapatite (HAp) of calcium-phosphate-hydroxide salt coated on the surface layer of the DN hydrogels induced spontaneous osteogenesis penetrating into the semi-permeable hydrogels to form a gel/bone composite layer. In this work, the 44 Ca isotope-doped HAp/DN hydrogel is implanted in a defect of rabbit femoral bone and the dynamic osteogenesis process at the gel/bone interface is analyzed by tracing the calcium isotope ratio using isotope microscopy. The synthetic HAp hybridized on the surface layer of DN gel dissolves rapidly in the first two weeks by inflammation, and then the immature bone with a gradient structure starts to form in the gel region, reutilizing the dissolved Ca ions. These results reveal, for the first time, that synthetic HAp is reutilized for osteogenesis. These facts help to understand the lifetime of bone absorbable materials and to elucidate the mechanism of spontaneous, non-toxic, but strong fixation of hydrogels to bones.
  • Preparation of Tough Double- And Triple-Network Supermacroporous Hydrogels through Repeated Cryogelation
    Tomáš Sedlačík, Takayuki Nonoyama, Honglei Guo, Ryuji Kiyama, Tasuku Nakajima, Yoshihiro Takeda, Takayuki Kurokawa, Jian Ping Gong
    Chemistry of Materials, 32, 19, 8576, 8586, 13 Oct. 2020, [Peer-reviewed]
    Scientific journal
  • Chitin-based double-network hydrogel as potential superficial soft tissue repairing materials.
    Junchao Huang, Martin Frauenlob, Yuki Shibata, Lei Wang, Tasuku Nakajima, Takayuki Nonoyama, Masumi Tsuda, Shinya Tanaka, Takayuki Kurokawa, Jian Ping Gong
    Biomacromolecules, 21, 10, 4220, 4230, 16 Sep. 2020, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Chitin is a biopolymer which has been proved to be a candidate as biomedical materials, yet the weak mechanical properties limited their potentials seriously. In this work, a chitin-based double-network (DN) hydrogel was designed as a potential superficial repairing material. The hydrogel was synthesized through double-network (DN) strategy composing hybrid regenerated chitin nanofibers (RCNs)-poly (ethylene glycol diglycidyl ether) (PEGDE) as the first network and polyacrylamide (PAAm) as the second network. The hybrid RCNs-PEGDE/PAAm DN hydrogel was strong and tough, possessing Young's modulus (elasticity) E 0.097 ± 0.020 MPa, fracture stress σf 0.449 ± 0.025 MPa, and work of fracture Wf 5.75 ± 0.35 MJ·m-3. The obtained DN hydrogel was strong enough for surgical requirements in the usage of soft tissue scaffolds. In addition, the chitin endowed the DN hydrogel with good bacteria resistance and accelerated fibroblast proliferation, which NIH3T3 cell number increased nearly 5 times within 3 days. Subcutaneous implantation studies showed that the DN hydrogel did not induce inflammation after 4 weeks, suggesting a good biosafety in vivo. These results indicated that the hybrid RCNs-PEGDE/PAAm DN hydrogel had great prospect as rapid soft tissue repairing materials.
  • Hydrogels toughened by biominerals providing energy-dissipative sacrificial bonds.
    Kazuki Fukao, Kazuki Tanaka, Ryuji Kiyama, Takayuki Nonoyama, Jian Ping Gong
    Journal of materials chemistry. B, 15 May 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Inspired by bone tissues, we mineralized low crystalline hydroxyapatite (HAp) particles in double network (DN) hydrogels, and we observed that the HAp minerals toughen the gels. The contribution of dissipated energy from HAp minerals was over 500% higher than that from the polymer during tensile deformation. We elucidated that the amorphous parts in the HAp minerals break at deformation, acting as energy-dissipative sacrificial bonds. This result implies that not only brittle polymer networks but also minerals can provide sacrificial bonds to toughen soft materials.
  • Meet the phase-separation polymeric gel　　　　　　　　　　　　　　　
    Takayuki Nonoyama, Jian Ping Gong
    The Innovation Platform, 1, 73, 74, Mar. 2020, [Invited]
    English, Scientific journal
  • Surface charge dominated protein absorption on hydrogels.
    Honglei Guo, Yuto Uehara, Takahiro Matsuda, Ryuji Kiyama, Long Li, Jamil Ahmed, Yoshinori Katsuyama, Takayuki Nonoyama, Takayuki Kurokawa
    Soft matter, 16, 7, 1897, 1907, 29 Jan. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal, Soft tissue engineering requires antifouling materials that are biocompatible and mechanically flexible. Conventional hydrogels containing more than 70 wt% water are thus promising antifouling material candidates. However, some hydrogels are difficult to apply in internal body organs because of undesirable protein absorption on their surfaces. Due to the lack of an effective method for observing the true charge densities of hydrogels, the reason why electrostatic interactions dominate protein absorption behavior remains unclear. In this work, we adopt the microelectrode technique (MET) to study the electrical potentials of hydrogels with negative, positive, and neutral potentials and demonstrate the protein absorption behaviors on those hydrogels. The results show that MET is an effective method to obtain the surface charge densities of various hydrogels. Furthermore, the amounts of absorbed proteins on the gels were quantified with respect to the charge densities of the hydrogels. The results indicate that electrostatic absorption is quantitatively dominated by a combination of hydrogel charge density and overall protein charge. Based on the knowledge obtained in this work, the effects of hydrogel surface charges on protein absorption can be better understood. Thus, the results are expected to promote the application of hydrogels in tissue engineering.
  • Instant Thermal Switching from Soft Hydrogel to Rigid Plastics Inspired by Thermophile Proteins
    Nonoyama, T., Lee, Y.W., Ota, K., Fujioka, K., Hong, W., Gong, J.P.
    Advanced Materials, 32, 4, e1905878, 2020, [Peer-reviewed], [Lead author, Corresponding author]
    Scientific journal
  • Erratum: Preparation of Tough Double- and Triple-Network Supermacroporous Hydrogels through Repeated Cryogelation (Chem. Mater. (2020) 32:19 (8576−8586) DOI: 10.1021/acs.chemmater.0c02911)
    Sedla?{\'i}k, T., Nonoyama, T., Guo, H., Kiyama, R., Nakajima, T., Takeda, Y., Kurokawa, T., Gong, J.P.
    Chemistry of Materials, 32, 24, 10737, 10737, 2020
    Scientific journal
  • Robust hydrogel–bioceramics composite and its osteoconductive properties
    Takayuki Nonoyama
    Polymer Journal, 2020, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
  • Effect of Relative Strength of Two Networks on the Internal Fracture Process of Double Network Hydrogels As Revealed by in Situ Small-Angle X-ray Scattering
    Kazuki Fukao, Tasuku Nakajima, Takayuki Nonoyama, Takayuki Kurokawa, Takahiko Kawai, Jian Ping Gong
    Macromolecules, 2020, [Peer-reviewed]
    Scientific journal
  • Effect of Structure Heterogeneity on Mechanical Performance of Physical Polyampholytes Hydrogels
    Cui Kunpeng, Ye Ya Nan, Sun Tao Lin, Chen Liang, Li Xueyu, Kurokawa Takayuki, Nakajima Tasuku, Nonoyama Takayuki, Gong Jian Ping
    MACROMOLECULES, 52, 19, 7369, 7378, 08 Oct. 2019, [Peer-reviewed]
    English, Scientific journal
  • Tough double network elastomers reinforced by the amorphous cellulose network
    Murai, J., Nakajima, T., Matsuda, T., Tsunoda, K., Nonoyama, T., Kurokawa, T., Gong, J.P.
    Polymer, 178, 12 Sep. 2019, [Peer-reviewed]
    Scientific journal
  • Tough Triblock Copolymer Hydrogels with Different Micromorphologies for Medical and Sensory Materials
    Zhang Hui Jie, Luo Feng, Ye Yanan, Sun Tao Lin, Nonoyama Takayuki, Kurokawa Takayuki, Nakajima Tasuku
    ACS APPLIED POLYMER MATERIALS, 1, 8, 1948, 1953, Aug. 2019, [Peer-reviewed]
    English, Scientific journal
  • Osteochondral Autograft Transplantation Technique Augmented by an Ultrapurified Alginate Gel Enhances Osteochondral Repair in a Rabbit Model.
    Ryosuke Hishimura, Tomohiro Onodera, Kazutoshi Hontani, Rikiya Baba, Kentaro Homan, Shinji Matsubara, Zenta Joutoku, WooYoung Kim, Takayuki Nonoyama, Takayuki Kurokawa, Jian Ping Gong, Norimasa Iwasaki
    The American journal of sports medicine, 47, 2, 468, 478, Feb. 2019, [Peer-reviewed], [International Magazine]
    English, Scientific journal, BACKGROUND: One of the most important limitations of osteochondral autograft transplantation (OAT) is the adverse effect on donor sites in the knee. To decrease the number and/or size of osteochondral defects, we devised a method with biomaterial implantation after OAT. HYPOTHESIS: OAT augmented by ultrapurified alginate (UPAL) gel enhances cartilage repair capacity. STUDY DESIGN: Controlled laboratory study. METHODS: Seventy-five osteochondral defects in rabbits were divided into 3 groups: osteochondral defects with OAT alone, defects with OAT augmented by UPAL gel (combined group), and defects without intervention as controls. Macroscopic and histological evaluations of the reparative tissues were performed at 4 and 12 weeks postoperatively. Histological evaluation of graft cartilage degradation was also performed. To evaluate the effects of UPAL gel on graft healing, repaired bone volumes and osseointegration of the graft were evaluated. Collagen orientation and the mechanical properties of the reparative tissue and graft cartilage were also evaluated qualitatively. RESULTS: The macroscopic and histological evaluations of the combined group were significantly superior to the other groups at 12 weeks postoperatively. Regarding degenerative change of the graft, the histological scores of the combined group were significantly higher than those of the OAT-alone group. The values of repaired subchondral bone volumes and osseointegration of the graft were almost identical in both groups. Collagen orientation and the mechanical properties of the reparative tissue and graft cartilage were significantly better in the combined group than in the other groups. CONCLUSION: Administration of UPAL gel in OAT enhanced cartilage repair and protected graft cartilage without inhibiting subchondral bone repair and graft survival. CLINICAL RELEVANCE: OAT augmented by UPAL gel decreases the number and/or size of osteochondral grafts, minimizing the risk of donor site morbidity. This combination technique has the potential to improve clinical outcomes and expand the surgical indications for OAT.
  • Tough Particle-Based Double Network Hydrogels for Functional Solid Surface Coatings
    Takahashi, R., Shimano, K., Okazaki, H., Kurokawa, T., Nakajima, T., Nonoyama, T., King, D.R., Gong, J.P.
    Advanced Materials Interfaces, 5, 23, 07 Dec. 2018, [Peer-reviewed]
    Scientific journal
  • An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study.
    Takeru Tsujimoto, Hideki Sudo, Masahiro Todoh, Katsuhisa Yamada, Koji Iwasaki, Takashi Ohnishi, Naoki Hirohama, Takayuki Nonoyama, Daisuke Ukeba, Katsuro Ura, Yoichi M Ito, Norimasa Iwasaki
    EBioMedicine, 37, 521, 534, Nov. 2018, [Peer-reviewed], [International Magazine]
    English, Scientific journal, BACKGROUND: The current surgical procedure of choice for lumbar intervertebral disc (IVD) herniation is discectomy. However, defects within IVD produced upon discectomy may impair tissue healing and predispose patients to subsequent IVD degeneration. This study aimed to investigate whether the use of an acellular bioresorbable ultra-purified alginate (UPAL) gel implantation system is safe and effective as a reparative therapeutic strategy after lumbar discectomy. METHODS: Human IVD cells were cultured in a three-dimensional system in UPAL gel. In addition, lumbar spines of sheep were used for mechanical analysis. Finally, the gel was implanted into IVD after discectomy in rabbits and sheep in vivo. FINDINGS: The UPAL gel was biocompatible with human IVD cells and promoted extracellular matrix production after discectomy, demonstrating sufficient biomechanical characteristics without material protrusion. INTERPRETATION: The present results indicate the safety and efficacy of UPAL gels in a large animal model and suggest that these gels represent a novel therapeutic strategy after discectomy in cases of lumbar IVD herniation. FUND: Grant-in-Aid for the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.
  • Micro patterning of hydroxyapatite by soft lithography on hydrogels for selective osteoconduction.
    Kiyama R, Nonoyama T, Wada S, Semba S, Kitamura N, Nakajima T, Kurokawa T, Yasuda K, Tanaka S, Gong JP
    Acta biomaterialia, 81, 60, 69, Oct. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal, Mechanically robust hydrogels are promising biomaterials as artificial supportive tissue. These applications require selective and robust bonding of the hydrogels to living tissue. Recently, we achieved strong in vivo bone bonding of a tough double network (DN) hydrogel, a potential material for use as artificial cartilage and tendon, by hybridizing osteoconductive hydroxyapatite (HAp) in the gel surface layer. In this work, we report micro patterning of HAp at the surface of the DN hydrogel for selective osteoconduction. Utilizing the dissolution of HAp in an acidic environment, the soft lithography technique using an acid gel stamp was adopted to form a high-resolution HAp pattern on the gel. The HAp-patterned gel showed well-regulated migration and adhesion of cells in vitro. Moreover, the HAp-patterned gel showed selective and robust bonding to the rabbit bone tissue in vivo. This HAp soft lithography technique allows for simple and quick preparation of tailor-made osteoconductive hydrogels and can be applied to other hydrogels for selective bone bonding. STATEMENT OF SIGNIFICANCE: Hydrogels, preserving large amount of water, have been studied for next-generation artificial soft tissues. However, fixation of hydrogels to living tissue was unsolved issue for clinical application. Recently, we achieved robust bonding of a tough double network gel to bone in vivo by coating of osteoconductive hydroxyapatite in the gel surface layer. For further progress for practical use, we report the micro patterning of HAp at the surface of the DN hydrogel by using soft lithography technique, to perform selective bonding to only objective area without unnecessary coalescence. The HAp lithography technique is simple, quick and non-toxic method to prepare tailor-made osteoconductive hydrogels and has universality of species of hydrogels.
  • Tough and Self-Recoverable Thin Hydrogel Membranes for Biological Applications
    Ye Ya Nan, Frauenlob Martin, Wang Lei, Tsuda Masumi, Sun Tao Lin, Cui Kunpeng, Takahashi Riku, Zhang Hui Jie, Nakajima Tasuku, Nonoyama Takayuki, Kurokawa Takayuki, Tanaka Shinya, Gong Jian Ping
    ADVANCED FUNCTIONAL MATERIALS, 28, 31, 01 Aug. 2018, [Peer-reviewed]
    English, Scientific journal
  • A Facile Method to Fabricate Anisotropic Hydrogels with Perfectly Aligned Hierarchical Fibrous Structures
    Md. Tariful Islam Mredha, Yun Zhou Guo, Takayuki Nonoyama, Tasuku Nakajima, Takayuki Kurokawa, Jian Ping Gong
    Advanced Materials, 30, 9, 01 Mar. 2018, [Peer-reviewed]
    English, Scientific journal
  • Tough and variable-band-gap photonic hydrogel displaying programmable angle-dependent colors
    Md. Anamul Haque, Kei Mito, Takayuki Kurokawa, Tasuku Nakajima, Takayuki Nonoyama, Muhammad Ilyas, Jian Ping Gong
    ACS Omega, 3, 1, 55, 62, 2018, [Peer-reviewed]
    English, Scientific journal
  • Anisotropic Growth of Hydroxyapatite in Stretched Double Network Hydrogel
    Kazuki Fukao, Takayuki Nonoyama, Ryuji Kiyama, Kazuya Furusawa, Takayuki Kurokawa, Tasuku Nakajima, Jian Ping Gong
    ACS Nano, 11, 12, 12103, 12110, American Chemical Society, 26 Dec. 2017, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Water-Triggered Ductile-Brittle Transition of Anisotropic Lamellar Hydrogels and Effect of Confinement on Polymer Dynamics
    Muhammad Ilyas, Md. Anamul Haque, Youfeng Yue, Takayuki Kurokawa, Tasuku Nakajima, Takayuki Nonoyama, Jian Ping Gong
    MACROMOLECULES, 50, 20, 8169, 8177, Oct. 2017, [Peer-reviewed]
    English, Scientific journal
  • Supramolecular hydrogels with multi-cylindrical lamellar bilayers: Swelling-induced contraction and anisotropic molecular diffusion
    Kei Mito, Md. Anamul Haque, Tasuku Nakajima, Maki Uchiumi, Takayuki Kurokawa, Takayuki Nonoyama, Jian Ping Gong
    POLYMER, 128, 373, 378, Oct. 2017, [Peer-reviewed]
    English, Scientific journal
  • Anisotropic tough double network hydrogel from fish collagen and its spontaneous in vivo bonding to bone
    Md. Tariful Islam Mredha, Nobuto Kitamura, Takayuki Nonoyama, Susumu Wada, Keiko Goto, Xi Zhang, Tasuku Nakajima, Takayuki Kurokawa, Yasuaki Takagi, Kazunori Yasuda, Jian Ping Gong
    BIOMATERIALS, 132, 85, 95, Jul. 2017, [Peer-reviewed]
    English, Scientific journal
  • Energy-Dissipative Matrices Enable Synergistic Toughening in Fiber Reinforced Soft Composites
    Yiwan Huang, Daniel R. King, Tao Lin Sun, Takayuki Nonoyama, Takayuki Kurokawa, Tasuku Nakajima, Jian Ping Gong
    ADVANCED FUNCTIONAL MATERIALS, 27, 9, 1605350, Mar. 2017, [Peer-reviewed]
    English, Scientific journal
  • Double Networkゲルをマトリックスとした骨組織類似構造の創製　　　　　　　　　　　　　　　
    深尾 一城, 野々山 貴行, 黒川 孝幸, 中島 祐, きょう 剣萍
    日本バイオマテリアル学会大会予稿集, シンポジウム2016, 237, 237, 日本バイオマテリアル学会, Nov. 2016
    Japanese
  • Hydroxyapatite-coated double network hydrogel directly bondable to the bone: Biological and biomechanical evaluations of the bonding property in an osteochondral defect
    Susumu Wada, Nobuto Kitamura, Takayuki Nonoyama, Ryuji Kiyama, Takayuki Kurokawa, Jian Ping Gong, Kazunori Yasuda
    ACTA BIOMATERIALIA, 44, 125, 134, Oct. 2016, [Peer-reviewed]
    English, Scientific journal
  • Fundamental biomaterial properties of tough glycosaminoglycan-containing double network hydrogels newly developed using the molecular stent method
    Kotaro Higa, Nobuto Kitamura, Takayuki Kurokawa, Keiko Goto, Susumu Wada, Takayuki Nonoyama, Fuminori Kanaya, Kazuyuki Sugahara, Jian Ping Gong, Kazunori Yasuda
    ACTA BIOMATERIALIA, 43, 38, 49, Oct. 2016, [Peer-reviewed]
    English, Scientific journal
  • ハイドロキシアパタイト複合化PAMPS/PDMAAmダブルネットワークゲル人工軟骨の関節内におけるin vivo骨接着性評価
    和田 進, 北村 信人, 野々山 貴行, 木山 竜二, 仙葉 愼吾, 小野寺 純, 横田 正司, 後藤 佳子, 比嘉 浩太郎, 黒川 孝幸, Gong Jian Ping, 安田 和則
    日本整形外科学会雑誌, 90, 8, S1598, S1598, (公社)日本整形外科学会, Aug. 2016
    Japanese
  • Creep Behavior and Delayed Fracture of Tough Polyampholyte Hydrogels by Tensile Test
    Sadia Nazneen Karobi, Tao Lin Sun, Takayuki Kurokawa, Feng Luo, Tasuku Nakajima, Takayuki Nonoyama, Jian Ping Gong
    MACROMOLECULES, 49, 15, 5630, 5636, Aug. 2016, [Peer-reviewed]
    English, Scientific journal
  • Double-Network Hydrogels Strongly Bondable to Bones by Spontaneous Osteogenesis Penetration
    Takayuki Nonoyama, Susumu Wada, Ryuji Kiyama, Nobuto Kitamura, Md. Tariful Islam Mredha, Xi Zhang, Takayuki Kurokawa, Tasuku Nakajima, Yasuaki Takagi, Kazunori Yasuda, Jian Ping Gong
    ADVANCED MATERIALS, 28, 31, 6740, +, Aug. 2016, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • Molecular Structure and Properties of Click Hydrogels with Controlled Dangling End Defect
    Ao-kai Zhang, Jun Ling, Kewen Li, Guo-dong Fu, Tasuku Nakajima, Takayuki Nonoyama, Takayuki Kurokawa, Jian Ping Gong
    JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 54, 13, 1227, 1236, Jul. 2016, [Peer-reviewed]
    English, Scientific journal
  • Self-Healing Behaviors of Tough Polyampholyte Hydrogels
    Abu Bin Ihsan, Tao Lin Sun, Takayuki Kurokawa, Sadia Nazneen Karobi, Tasuku Nakajima, Takayuki Nonoyama, Chanchal Kumar Roy, Feng Luo, Jian Ping Gong
    MACROMOLECULES, 49, 11, 4245, 4252, Jun. 2016, [Peer-reviewed]
    English, Scientific journal
  • Tough Physical Double-Network Hydrogels Based on Amphiphilic Triblock Copolymers
    Hui Jie Zhang, Tao Lin Sun, Ao Kai Zhang, Yumihiko Ikura, Tasuku Nakajima, Takayuki Nonoyama, Takayuki Kurokawa, Osamu Ito, Hiroyuki Ishitobi, Jian Ping Gong
    ADVANCED MATERIALS, 28, 24, 4884, 4890, Jun. 2016, [Peer-reviewed]
    English, Scientific journal
  • Quantitative Observation of Electric Potential Distribution of Brittle Polyelectrolyte Hydrogels Using Microelectrode Technique
    Honglei Guo, Takayuki Kurokawa, Masakazu Takahata, Wei Hong, Yoshinori Katsuyama, Feng Luo, Jamil Ahmed, Tasuku Nakajima, Takayuki Nonoyama, Jian Ping Gong
    MACROMOLECULES, 49, 8, 3100, 3108, Apr. 2016, [Peer-reviewed]
    English, Scientific journal
  • Structure Transition and Function Modification of a Hydrogel Based on Lamellar Bilayers
    Kei Mito, Tasuku Nakajima, Takayuki Nonoyama, Jian Ping Gong
    KOBUNSHI RONBUNSHU, 73, 2, 157, 165, 2016, [Peer-reviewed]
    Japanese, Scientific journal
  • Stretching-induced ion complexation in physical polyampholyte hydrogels
    Kunpeng Cui, Tao Lin Sun, Takayuki Kurokawa, Tasuku Nakajima, Takayuki Nonoyama, Liang Chen, Jian Ping Gong
    SOFT MATTER, 12, 43, 8833, 8840, 2016, [Peer-reviewed]
    English, Scientific journal
  • Coupled instabilities of surface crease and bulk bending during fast free swelling of hydrogels
    Riku Takahashi, Yumihiko Ikura, Daniel R. King, Takayuki Nonoyama, Tasuku Nakajima, Takayuki Kurokawa, Hirotoshi Kuroda, Yoshihiro Tonegawa, Jian Ping Gong
    SOFT MATTER, 12, 23, 5081, 5088, 2016, [Peer-reviewed]
    English, Scientific journal
  • Double-network hydrogel and its potential biomedical application: A review
    Takayuki Nonoyama, Jian Ping Gong
    PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE, 229, 12, 853, 863, Dec. 2015, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • Self-Adjustable Adhesion of Polyampholyte Hydrogels
    Chanchal Kumar Roy, Hong Lei Guo, Tao Lin Sun, Abu Bin Ihsan, Takayuki Kurokawa, Masakazu Takahata, Takayuki Nonoyama, Tasuku Nakajima, Jian Ping Gong
    ADVANCED MATERIALS, 27, 45, 7344, +, Dec. 2015, [Peer-reviewed]
    English, Scientific journal
  • Extremely tough composites from fabric reinforced polyampholyte hydrogels
    Daniel R. King, Tao Lin Sun, Yiwan Huang, Takayuki Kurokawa, Takayuki Nonoyama, Alfred J. Crosby, Jian Ping Gong
    MATERIALS HORIZONS, 2, 6, 584, 591, Nov. 2015, [Peer-reviewed]
    English, Scientific journal
  • Phase-Separation-Induced Anomalous Stiffening, Toughening, and Self-Healing of Polyacrylamide Gels
    Koshiro Sato, Tasuku Nakajima, Toshiyuki Hisamatsu, Takayuki Nonoyama, Takayuki Kurokawa, Jian Ping Gong
    ADVANCED MATERIALS, 27, 43, 6990, +, Nov. 2015, [Peer-reviewed]
    English, Scientific journal
  • バイオミネラリゼーションにより複合化したハイドロキシアパタイトコーティングPAMPS/PDMAAmダブルネットワークゲルの骨接着性の評価
    和田 進, 北村 信人, 野々山 貴行, 木山 竜二, 仙葉 愼吾, 比嘉 浩太郎, 黒川 孝幸, Gong Jian Ping, 安田 和則
    日本整形外科学会雑誌, 89, 8, S1585, S1585, (公社)日本整形外科学会, Sep. 2015
    Japanese
  • Friction of Zwitterionic Hydrogel by Dynamic Polymer Adsorption
    Jamil Ahmed, Tetsurou Yamamoto, Honglei Guo, Takayuki Kurokawa, Takayuki Nonoyama, Tasuku Nakajima, Jian Ping Gong
    MACROMOLECULES, 48, 15, 5394, 5401, Aug. 2015, [Peer-reviewed]
    English, Scientific journal
  • Swim bladder collagen forms hydrogel with macroscopic superstructure by diffusion induced fast gelation
    Md. Tariful Islam Mredha, Xi Zhang, Takayuki Nonoyama, Tasuku Nakajima, Takayuki Kurokawa, Yasuaki Takagid, Jian Ping Gong
    JOURNAL OF MATERIALS CHEMISTRY B, 3, 39, 7658, 7666, 2015, [Peer-reviewed]
    English, Scientific journal
  • Control superstructure of rigid polyelectrolytes in oppositely charged hydrogels via programmed internal stress
    Riku Takahashi, Zi Liang Wu, Md Arifuzzaman, Takayuki Nonoyama, Tasuku Nakajima, Takayuki Kurokawa, Jian Ping Gong
    NATURE COMMUNICATIONS, 5, 4490, Aug. 2014, [Peer-reviewed]
    English, Scientific journal
  • Mechano-actuated ultrafast full-colour switching in layered photonic hydrogels
    Youfeng Yue, Takayuki Kurokawa, Md Anamul Haque, Tasuku Nakajima, Takayuki Nonoyama, Xufeng Li, Itsuro Kajiwara, Jian Ping Gong
    NATURE COMMUNICATIONS, 5, 4659, Aug. 2014, [Peer-reviewed]
    English, Scientific journal
  • Double network hydrogels from polyzwitterions: high mechanical strength and excellent anti-biofouling properties
    Haiyan Yin, Taigo Akasaki, Tao Lin Sun, Tasuku Nakajima, Takayuki Kurokawa, Takayuki Nonoyama, Toshio Taira, Yoshiyuki Saruwatari, Jian Ping Gong
    JOURNAL OF MATERIALS CHEMISTRY B, 1, 30, 3685, 3693, 2013, [Peer-reviewed]
    English, Scientific journal
  • Arrangement techniques of proteins and cells using amorphous calcium phosphate nanofiber scaffolds
    Takayuki Nonoyama, Takatoshi Kinoshita, Masahiro Higuchi, Kenji Nagata, Masayoshi Tanaka, Mari Kamada, Kimiyasu Sato, Katsuya Kato
    APPLIED SURFACE SCIENCE, 262, 8, 12, Dec. 2012, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • Structural Formation Ability of Peptide Secondary Structure on Silica Biomineralization
    Tatsuya Kuno, Takayuki Nonoyama, Kiyoshi Hirao, Katsuya Kato
    CHEMISTRY LETTERS, 41, 11, 1547, 1549, Nov. 2012, [Peer-reviewed]
    English, Scientific journal
  • TiO2 Synthesis Inspired by Biomineralization: Control of Morphology, Crystal Phase, and Light-Use Efficiency in a Single Process
    Takayuki Nonoyama, Takatoshi Kinoshita, Masahiro Higuchi, Kenji Nagata, Masayoshi Tanaka, Kimiyasu Sato, Katsuya Kato
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134, 21, 8841, 8847, May 2012, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • Enzyme structure and catalytic properties affected by the surface functional groups of mesoporous silica
    Kazuki Murai, Takayuki Nonoyama, Takao Saito, Katsuya Kato
    CATALYSIS SCIENCE & TECHNOLOGY, 2, 2, 310, 315, 2012, [Peer-reviewed]
    English, Scientific journal
  • Calcium phosphate biomineralization in peptide hydrogels for injectable bone-filling materials
    Takayuki Nonoyama, Hokuto Ogasawara, Masayoshi Tanaka, Masahiro Higuchi, Takatoshi Kinoshita
    SOFT MATTER, 8, 45, 11531, 11536, 2012, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • Influence of the Charge Relay Effect on the Silanol Condensation Reaction as a Model for Silica Biomineralization
    Tatsuya Kuno, Takayuki Nonoyama, Kiyoshi Hirao, Katsuya Kato
    LANGMUIR, 27, 21, 13154, 13158, Nov. 2011, [Peer-reviewed]
    English, Scientific journal
  • Ordered Nanopattern Arrangement of Gold Nanoparticles on beta-Sheet Peptide Templates through Nucleobase Pairing
    Takayuki Nonoyama, Masayoshi Tanaka, Yoshihito Inai, Masahiro Higuchi, Takatoshi Kinoshita
    ACS NANO, 5, 8, 6174, 6183, Aug. 2011, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • Multistep Growth Mechanism of Calcium Phosphate in the Earliest Stage of Morphology-Controlled Biomineralization
    Takayuki Nonoyama, Takatoshi Kinoshita, Masahiro Higuchi, Kenji Nagata, Masayoshi Tanaka, Kimiyasu Sato, Katsuya Kato
    LANGMUIR, 27, 11, 7077, 7083, Jun. 2011, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • Morphology control of calcium phosphate by mineralization on the beta-sheet peptide template
    Takayuki Nonoyama, Masayoshi Tanaka, Takatoshi Kinoshita, Fukue Nagata, Kimiyasu Sato, Katsuya Kato
    CHEMICAL COMMUNICATIONS, 46, 37, 6983, 6985, Oct. 2010, [Peer-reviewed], [Lead author]
    English, Scientific journal

Other Activities and Achievements

Books and other publications

Lectures, oral presentations, etc.

  • セラミックスを犠牲結合部位としたハイドロゲルの高靭性化
    田中香月, 野々山貴行, 深尾一城, 木山竜二, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 09 Jan. 2019, Japanese
  • 骨再生を促す高靭性ゲルの同位体顕微鏡法による進展評価
    鈴木裕貴, 野々山貴行, 野々山貴行, WANG Lei, WANG Lei, 津田真寿美, 津田真寿美, 木山竜二, 安田和則, 安田和則, GONG Jian Ping, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 09 Jan. 2019, Japanese
  • 家兎半月板部分欠損モデルにおける高純度硬化性アルギン酸ゲル移植の半月板修復に与える効果
    金佑泳, 小野寺智洋, 近藤英司, 野々山貴行, 馬場力哉, 上徳善太, 宝満健太郎, 菱村亮介, 岩崎倫政
    整形外科バイオマテリアル研究会プログラム・抄録集, Nov. 2018, Japanese
  • 温度依存性がない物理ゲルの創製
    藤岡慶伍, 野々山貴行, 野々山貴行, GUO Honglei, GUO Honglei, GUO Hui, GUO Hui, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • 新規高分子鎖ナノスケール直接観察法を用いたハイドロゲル表面構造解析
    木山竜二, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • 弾性率超転移を示す可逆的温度応答性ハイドロゲルの創製
    野々山貴行, 野々山貴行, LEE Yong Woo, 太田玖美, 藤岡慶伍, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • DNゲルのヒステリシス解析による単一高分子鎖の挙動抽出
    高橋由葵子, 松田昂大, 中島祐, 中島祐, 印出井努, 印出井努, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • 同位体顕微鏡法による高靭性ゲルへの骨再生進展の評価
    鈴木裕貴, 野々山貴行, 野々山貴行, WANG Lei, WANG Lei, 津田真寿美, 津田真寿美, 木山竜二, 安田和則, 安田和則, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • 巨視的な犠牲結合を有するソフト複合材料の高靭性化
    奥村剛士, 高橋陸, KING Daniel R, KING Daniel R, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • 企業訪問の取り組みと高分子未来塾
    野々山貴行
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • 一軸延伸過程におけるダブルネットワークゲルのin‐situ小角X線散乱
    深尾一城, 野々山貴行, 中島祐, 黒川孝幸, 河井貴彦, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • 機械刺激が誘起するDNゲルの自己強靭化
    難波遼, 松田昂大, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 29 Aug. 2018, Japanese
  • ダブルネットワーク原理をマクロスケールに拡張した複合材料の創製及び物性制御
    奥村剛士, 高橋陸, KING Daniel R, KING Daniel R, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 08 May 2018, Japanese
  • X線散乱法によるDouble Networkゲルの延伸過程における内部構造の評価
    深尾一城, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 08 May 2018, Japanese
  • 無機粒子複合化によるハイドロゲル高分子鎖の新規直接観察法の開発
    木山竜二, 野々山貴行, 中島祐, 黒川孝幸, GONG Jian Ping
    日本セラミックス協会年会講演予稿集(CD-ROM), 01 Mar. 2018, Japanese
  • 伸長下高強度ハイドロゲル内におけるハイドロキシアパタイトの異方的結晶成長
    深尾一城, 野々山貴行, 古澤和也, 黒川孝幸, 中島祐, GONG Jian Ping
    日本セラミックス協会年会講演予稿集(CD-ROM), 01 Mar. 2018, Japanese
  • 巨視的なダブルネットワーク構造を有する複合材料の創製及び物性評価
    奥村剛士, 高橋陸, 黒川孝幸, 黒川孝幸, KING Daniel R, KING Daniel R, 中島祐, 中島祐, 孫桃林, 孫桃林, 野々山貴行, 野々山貴行, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 15 Jan. 2018, Japanese
  • バイオセラミックスを犠牲結合としたハイドロゲルの高靭化
    田中香月, 野々山貴行, 野々山貴行, 木山竜二, 深尾一城, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 15 Jan. 2018, Japanese
  • メカノケミカル重合反応による自己強靭化DNゲルの創製
    難波遼, 松田昂大, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 15 Jan. 2018, Japanese
  • 体積相転移の無いLCSTと可逆的熱硬化能を有する新規ハイドロゲルの創製
    李永祐, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 15 Jan. 2018, Japanese
  • ずり流動場での二重膜ハイブリッドゲルの合成とその異方構造解析
    井上あかね, MD.ANAMUL Haque, 黒川孝幸, 黒川孝幸, 中島祐, 中島祐, 野々山貴行, 野々山貴行, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 15 Jan. 2018, Japanese
  • 高温で高強度・高靭性化するアイソコリック相分離ゲルの創製
    野々山貴行, 野々山貴行, 太田玖美, LEE Yong Woo, 藤岡慶伍, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 04 Jan. 2018, Japanese
  • Direct observation of hydrogel nano-scale network structure
    木山竜二, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 04 Jan. 2018, Japanese
  • 半剛直性高分子を用いた強靭・異方的ダブルネットワークゲルの創製
    猪飼拓真, 高橋陸, キング ダニエル, キング ダニエル, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, グン剣萍, グン剣萍
    化学系学協会北海道支部冬季研究発表会(Web), 2018, Japanese
  • ハイドロゲルの潤滑におけるリンクル表面形状の効果
    黒川孝幸, 野々山貴行, 中島祐, GONG J. P, 小池智, 平井悠司, 大園拓哉
    レオロジー討論会講演要旨集, 17 Oct. 2017, Japanese
  • 破断誘起ラジカル重合による自己強靭化DNゲルの創製
    難波遼, 松田昂大, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 06 Sep. 2017, Japanese
  • 巨視的な二重網目構造を有する高強度・高靱性複合材料の創製及び制御
    奥村剛士, 高橋陸, KING Daniel R, KING Daniel R, 中島祐, 中島祐, SUN Taolin, SUN Taolin, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 06 Sep. 2017, Japanese
  • 生体分子イオン液体をベースとする生体分子イオンゲルの創製
    村井一喜, 楜澤和也, 大塚渓斗, 野々山貴行, 松本睦良
    高分子学会予稿集(CD-ROM), 06 Sep. 2017, Japanese
  • 多彩な構造色を有する二分子膜ゲルの構造精密制御と配向分析
    井上あかね, ANAMUL Haque Md, 黒川孝幸, 黒川孝幸, 中島祐, 中島祐, 野々山貴行, 野々山貴行, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 06 Sep. 2017, Japanese
  • ポーラス構造を有する強靭な高分子電解質複合体ゲルの創製と機能発現
    村川航平, SUN Taolin, SUN Taolin, KING Daniel R, KING Daniel R, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 06 Sep. 2017, Japanese
  • 高純度硬化性アルギン酸ゲル移植は家兎半月板部分欠損モデルにおいて半月板修復を促進する
    KIM W.Y, 近藤英司, 小野寺智洋, 野々山貴行, 馬場力哉, 本谷和俊, 上徳善太, 松原新史, 宝満健太郎, 菱村亮介, TERKAWI Muhamad, 岩崎倫政
    日本整形外科学会雑誌, 31 Aug. 2017, Japanese
  • ダブルネットワーク原理をマクロスケールに拡張した新規複合材料の創製
    奥村剛士, 高橋陸, KING Daniel R, KING Daniel R, 中島祐, 中島祐, SUN Taolin, SUN Taolin, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 15 May 2017, Japanese
  • 低融点合金を用いた機能性ハイドロゲル複合材料の創製
    高橋陸, DANIEL King, DANIEL King, 孫桃林, 孫桃林, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 15 May 2017, Japanese
  • Isochoric Hydrogels Possessing Thermal Hardening and Toughening
    野々山貴行, 野々山貴行, 太田玖美, LEE Yong Woo, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 15 May 2017, Japanese
  • 人工腱を目指した強配向・高強度生体高分子ゲルの創製
    中島祐, 中島祐, MREDHA Md.Tariful Islam, 黒川孝幸, 黒川孝幸, 野々山貴行, 野々山貴行, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 15 May 2017, Japanese
  • 生体分子のみを構成イオン種とするイオン液体の創成とイオン構造が液体特性に与える影響
    村井一喜, 楜澤和也, 大塚渓斗, 野々山貴行, 松本睦良
    高分子学会予稿集(CD-ROM), 15 May 2017, Japanese
  • 自己修復性を有する高温で強靭化する新規アイソコリックゲルの創製
    LEE Yong Woo, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 15 May 2017, Japanese
  • 電荷を有する微粒子ゲルの潤滑機構
    福田泰紀, 黒川孝幸, 黒川孝幸, KING Daniel R, KING Daniel R, 中島祐, 中島祐, 野々山貴行, 野々山貴行, SUN Taolin, SUN Taolin, GONG Jian Ping, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 06 Jan. 2017, Japanese
  • ポーラス構造を有するポリイオンコンプレックスゲルとその機能発現
    村川航平, SUN Taolin, SUN Taolin, KING Daniel R, KING Daniel R, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 06 Jan. 2017, Japanese
  • 破断誘起ラジカル重合による劣化部位修復性DNゲルの創製
    難波遼, 松田昂大, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 06 Jan. 2017, Japanese
  • 破断誘起ラジカル重合による劣化部位修復性DNゲルの創製
    難波遼, 松田昂大, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • 二分子膜からなるマルチシリンダーゲルの創製及び異方的挙動の解析
    水戸京, 李旭峰, HAQUE Md. Anamul, 黒川孝幸, 黒川孝幸, 中島祐, 中島祐, 野々山貴行, 野々山貴行, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • マクロスケールの二重網目構造を有する高強度・高靭性複合材料の創成及び制御
    奥村剛士, 高橋陸, 黒川孝幸, 黒川孝幸, KING Daniel R, KING Daniel R, 中島祐, 中島祐, 孫桃林, 孫桃林, 野々山貴行, 野々山貴行, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • チタン属イオン架橋を用いた強靭な異方性ハイドロゲルの創製
    猪飼拓真, 高橋陸, キング ダニエル, キング ダニエル, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • ポーラス構造を有するポリイオンコンプレックスゲルとその機能発現
    村川航平, 孫桃林, 孫桃林, KING ダニエル R, KING ダニエル R, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • 粘性エネルギーロスを示さないDouble Networkエラストマーの創製
    尾崎雄平, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • ネットワークの異方性が誘起するバイオミネラルの配向制御
    深尾一城, 野々山貴行, 野々山貴行, 古澤和也, 古澤和也, 黒川孝幸, 黒川孝幸, 中島祐, 中島祐, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • 電荷を有する微粒子ゲルの摩擦低減効果
    福田泰紀, 黒川孝幸, 黒川孝幸, キング ダニエル, キング ダニエル, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 孫桃林, 孫桃林, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • 高温で強靭化する新規アイソコリックハイドロゲルの創製
    太田玖美, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • セラミックスを犠牲結合部位としたハイドロゲルの高靱性化
    田中香月, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, きょう剣萍, きょう剣萍
    高分子学会北海道支部研究発表会講演要旨集, 2017, Japanese
  • Double Networkゲルをマトリックスとした骨組織類似構造の創製
    深尾一城, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, 中島祐, 中島祐, きょう剣萍, きょう剣萍
    日本バイオマテリアル学会シンポジウム予稿集, 21 Nov. 2016, Japanese
  • 傾斜構造による骨と直接結合する高靱性ハイドロゲル
    野々山貴行, 和田進, 木山竜二, 北村信人, 北村信人, 安田和則, きょう剣萍, きょう剣萍
    日本バイオマテリアル学会シンポジウム予稿集, 21 Nov. 2016, Japanese
  • ハイドロキシアパタイト複合化PAMPS/PDMAAmダブルネットワークゲル人工軟骨の関節内におけるin vivo骨接着性評価
    和田進, 北村信人, 野々山貴行, 木山竜二, 仙葉愼吾, 小野寺純, 横田正司, 後藤佳子, 比嘉浩太郎, 黒川孝幸, PING Gong Jian, 安田和則
    日本整形外科学会雑誌, 31 Aug. 2016, Japanese
  • 軟骨基質高分子を複合化したダブルネットワークゲルの生体材料特性評価:軟骨再生誘導材料としての可能性
    比嘉浩太郎, 北村信人, 後藤佳子, 黒川孝幸, 和田進, 野々山貴行, PING Gong Jian, 金谷文則, 安田和則
    日本整形外科学会雑誌, 31 Aug. 2016, Japanese
  • 高強度ハイドロゲル中でのハイドロキシアパタイトの異方的結晶成長
    深尾一城, 野々山貴行, 黒川孝幸, 中島祐, GONG Jian Ping
    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM), 29 Aug. 2016, Japanese
  • 軟骨再生DNゲルの骨接着に向けた表面選択的HApパターニング
    木山竜二, 野々山貴行, 中島祐, 黒川孝幸, GONG JianPing
    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM), 29 Aug. 2016, Japanese
  • 骨と直接結合するタフハイドロゲル
    野々山貴行, 野々山貴行, 和田進, 木山竜二, 北村信人, 北村信人, 黒川孝幸, 黒川孝幸, 中島祐, 中島祐, 安田和則, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 24 Aug. 2016, Japanese
  • 低融点合金を用いた機能性コンポジットゲルの創製
    高橋陸, DANIEL King, DANIEL King, 野々山貴行, 野々山貴行, 中島祐, 中島祐, 黒川孝幸, 黒川孝幸, GONG Jian Ping, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 24 Aug. 2016, Japanese
  • 遷移金属イオン架橋による強靭なハイドロゲルの創製
    猪飼拓真, 高橋陸, KING Daniel R, 中島祐, 野々山貴行, 黒川孝幸, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 24 Aug. 2016, Japanese
  • 生体分子のみから構成される新規イオン液体およびイオンゲルの創成
    大塚渓斗, 村井一喜, 野々山貴行, 松本睦良
    高分子学会予稿集(CD-ROM), 24 Aug. 2016, Japanese
  • 粘性によるエネルギーロスを示さない強靭エラストマーの創製
    尾崎雄平, 中島祐, 中島祐, 野々山貴行, 野々山貴行, 黒川孝幸, 黒川孝幸, CONG Jian Ping, CONG Jian Ping
    高分子学会予稿集(CD-ROM), 24 Aug. 2016, Japanese
  • Diffusion perturbation induced superstructure formation in biopolymer hydrogels
    MREDHA Md. Tariful Islam, ZHANG Xi, NONOYAMA Takayuki, NAKAJIMA Tasuku, KUROKAWA Takayuki, HAQUE Md. Anamul, TAKAGI Yasuaki, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 08 Jan. 2016, English
  • 半剛直性高分子電解質と金属多価イオンを用いた高強度ゲルの創製および配向の制御
    猪飼拓真, 高橋陸, 中島祐, 野々山貴行, 黒川孝幸, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 08 Jan. 2016, Japanese
  • 微粒子ゲルの摩擦低減効果
    福田泰紀, 黒川孝幸, 中島祐, 野々山貴行, GONG Jian Ping
    高分子ゲル研究討論会講演要旨集, 08 Jan. 2016, Japanese
  • Slow Relaxation Dynamics of Tough and Self-healing Polyampholyte Hydrogels
    KAROBI Sadia Nazneen, SUN Tao Lin, KUROKAWA Takayuki, LUO Feng, NAKAJIMA Tasuku, NONOYAMA Takayuki, GONG Jian Ping
    レオロジー討論会講演要旨集, 23 Sep. 2015, English
  • バイオミネラリゼーションにより複合化したハイドロキシアパタイトコーティングPAMPS/PDMAAmダブルネットワークゲルの骨接着性の評価
    和田進, 北村信人, 野々山貴行, 木山竜二, 仙葉愼吾, 比嘉浩太郎, 黒川孝幸, GONG Jian Ping, 安田和則
    日本整形外科学会雑誌, 10 Sep. 2015, Japanese
  • Tough physical double network hydrogels based on amphiphilic tri-block copolymer
    ZHANG Huijie, SUN Tao Lin, IKURA Yumihiko, ZHANG Aokai, LI Xufeng, NAKAJIMA Tasuku, NONOYAMA Takayuki, KURUKAWA Takayuki, ITO Osamu, ISHITOBI Hiroyuki, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 25 Aug. 2015, English
  • Precise Control of Gel Structures and Properties with Dangling End Defect
    ZHANG Aokai, NAKAJIMA Tasuku, KUROKAWA Takayuki, NONOYAMA Takayuki, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 25 Aug. 2015, English
  • The counter-ion condensation behavior of polyelectrolyte gels as studied by microelectrode technique (MET)
    GUO Honglei, KUROKAWA Takayuki, TAKAHATA Masakazu, KATSUYAMA Yoshinori, LUO Feng, AHMED Jamil, NAKAJIMA Tasuku, NONOYAMA Takayuki, GONG JianPing
    高分子学会予稿集(CD-ROM), 25 Aug. 2015, English
  • Polyampholyte hydrogels; smart, soft and wet adhesive
    ROY Chanchal Kumar, GUO Hong Lei, IHSAN Abu Bin, SUN Tao Lin, NONOYAMA Takayuki, NAKAJIMA Tasuku, KUROKAWA Takayuki, GONG Jian Ping
    高分子学会予稿集(CD-ROM), 25 Aug. 2015, English
  • Diffusion induced superstructure formation in swim bladder collagen　　　　　　　　　　　　　　　
    MREDHA MD. TARIFUL ISLAM, ZHANG XI, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, TAKAGI YASUAKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, English
  • 銀コーティングを用いた伸縮可能導電性ハイドロゲルの創製　　　　　　　　　　　　　　　
    TAKAHASHI RIKU, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, Japanese
  • セルロースを犠牲結合とする高靱性・自己修復性エラストマーの創製　　　　　　　　　　　　　　　
    MURAI JOJI, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, Japanese
  • 骨組織との接着を目指したDNゲル表面のHApパターニング　　　　　　　　　　　　　　　
    KIYAMA RYUJI, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, Japanese
  • Tough and Self-healing Polyampholyte Hydrogels-Similarity with Glassy Polymers　　　　　　　　　　　　　　　
    IHSAN ABU BIN, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, English
  • Abnormal elastic behaviour of poly(2-ureidoethyl methacrylate) physical hydrogels　　　　　　　　　　　　　　　
    SUN TAOLIN, NONOYAMA TAKAYUKI, SARUWATARI YOSHIYUKI, LUO FENG, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, IHSAN ABU BIN, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, English
  • Tough hydrogels with H-bonds as reversible sacrificial bonds　　　　　　　　　　　　　　　
    SUN TAOLIN, NONOYAMA TAKAYUKI, SARUWATARI YOSHIYUKI, LUO FENG, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, IHSAN ABU BIN, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, English
  • ダブルネットワークゲルの降伏メカニズムの解明　　　　　　　　　　　　　　　
    MATSUDA TAKAHIRO, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, NONOYAMA TAKAYUKI, SAKAI TAKAMASA, CHUNG UNG-IL, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, Japanese
  • Bioadhesion Using Polyampholyte Hydrogel　　　　　　　　　　　　　　　
    ROY CHANCHAL KUMAR, IHSAN ABU BIN, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 12 May 2015, English
  • 軟骨再生DNハイドロゲルの骨組織への接着を目指した表面選択的HApパターニング　　　　　　　　　　　　　　　
    KIYAMA RYUJI, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, GONG JIAN PING
    日本セラミックス協会年会講演予稿集(CD-ROM), 06 Mar. 2015, Japanese
  • DNハイドロゲルの大変形下におけるHApのミネラリゼーション　　　　　　　　　　　　　　　
    FUKAO KAZUKI, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, GONG JIAN PING
    日本セラミックス協会年会講演予稿集(CD-ROM), 06 Mar. 2015, Japanese
  • ハイドロゲルの摩擦と潤滑に及ぼす表面溝形状の効果　　　　　　　　　　　　　　　
    KOIKE SATOSHI, OZONO TAKUYA, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子ゲル研究討論会講演要旨集, 09 Jan. 2015, Japanese
  • 軟骨再生ハイドロゲルと骨組織の接着を目指した高靱性ハイドロゲル表面におけるHApのバイオミネラリゼーション　　　　　　　　　　　　　　　
    KIYAMA RYUJI, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, GONG JIAN PING
    高分子ゲル研究討論会講演要旨集, 09 Jan. 2015, Japanese
  • マルチシリンダー構造をもつ二分子膜ゲルの機能発現　　　　　　　　　　　　　　　
    MITO KEI, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, GONG JIAN PING
    高分子ゲル研究討論会講演要旨集, 09 Jan. 2015, Japanese
  • セルロースを犠牲結合とする高靱性・自己回復性ソフトマテリアルの創製　　　　　　　　　　　　　　　
    MURAI JOJI, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, KYO KENHEI
    高分子学会北海道支部研究発表会講演要旨集, 2015, Japanese
  • 力学的刺激が誘起するDNゲル内部の溶液化学反応　　　　　　　　　　　　　　　
    MATSUDA KODAI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, NONOYAMA TAKAYUKI, KYO KENHEI
    高分子学会北海道支部研究発表会講演要旨集, 2015, Japanese
  • Solvent induced photonic on/off swithing in lamellar bilayer hydrogel　　　　　　　　　　　　　　　
    ILYAS MUHAMMAD, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, GONG JIANPING
    高分子学会北海道支部研究発表会講演要旨集, 2015, English
  • 海水を給水源としたゲル上の植物成長システムの開発　　　　　　　　　　　　　　　
    OTAKE SHUNSUKE, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, MIWA KYOKO, KYO KENHEI
    高分子学会北海道支部研究発表会講演要旨集, 2015, Japanese
  • 両性電解質高分子を用いた繊維状高靱性ゲルの創製　　　　　　　　　　　　　　　
    HOSHINO KEN'ICHI, OKADA MASANORI, SON TORIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, KYO KENHEI
    高分子学会北海道支部研究発表会講演要旨集, 2015, Japanese
  • 抗菌性ゲルのスクリーニング　　　　　　　　　　　　　　　
    SHIBATA YUKI, KUROKAWA TAKAYUKI, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, AIZAWA TOMOYASU, KYO KENHEI
    高分子学会北海道支部研究発表会講演要旨集, 2015, Japanese
  • Two-step polymerization: a novel method for preparing polymer-supported lipid bilayer hydrogel　　　　　　　　　　　　　　　
    LI XU FENG, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 03 Sep. 2014, English
  • ダブルネットワークゲルの高分子鎖内部破断の化学的証明　　　　　　　　　　　　　　　
    MATSUDA TAKAHIRO, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, NONOYAMA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 03 Sep. 2014, Japanese
  • Tough and Self-healing Hydrogels from Polyampholytes Based on Reversible Sacrificial Bonds　　　　　　　　　　　　　　　
    IHSAN ABU BIN, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 03 Sep. 2014, English
  • 結合性骨形成を目指したHApコート高強度DNハイドロゲル最表面の構造評価　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KIYAMA RYUJI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 03 Sep. 2014, Japanese
  • Tough and Self-healing Hydrogels from Polyampholytes: Play with Reversible Sacrificial Bonds　　　　　　　　　　　　　　　
    IHSAN ABU BIN, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 09 May 2014, English
  • DNゲルの強靱性に対する数理的考察　　　　　　　　　　　　　　　
    IKURA YUMIHIKO, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, GONG JIAN PING, NAGAYAMA MASAHARU
    高分子学会予稿集(CD-ROM), 09 May 2014, Japanese
  • Robust Adhesion of Tough DN Hydrogels on Rough Solid Substrates by 'Graft Gelation'　　　　　　　　　　　　　　　
    OKAZAKI HARUKA, NAKAJIMA TASUKU, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 09 May 2014, English
  • 高強度ハイドロゲルとバイオセラミックスの融合:ソフトセラミックスの創製　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KIYAMA RYUJI, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, GONG JIAN PING
    日本セラミックス協会年会講演予稿集(CD-ROM), 07 Mar. 2014, Japanese
  • 軟骨再生ハイドロゲルと骨組織の接着を目指した高靱性DNゲル表面におけるHApのバイオミネラリゼーション　　　　　　　　　　　　　　　
    KIYAMA RYUJI, NONOYAMA TAKAYUKI, KUROKAWA TAKAYUKI, NAKAJIMA TASUKU, GONG JIAN PING
    日本セラミックス協会年会講演予稿集(CD-ROM), 07 Mar. 2014, Japanese
  • ゲルの異方的膨潤が誘起する液晶性高分子の超構造形成　　　　　　　　　　　　　　　
    TAKAHASHI RIKU, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, NONOYAMA TAKAYUKI, GUN CHEMPIN
    化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 28 Jan. 2014, Japanese
  • A novel anisotropic and tough hydrogel from swim bladder collagen　　　　　　　　　　　　　　　
    MREDHA MD. TARIFUL ISLAM, ZHANG XI, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, HAQUE MD. ANAMUL, TAKAGI YASUAKI, GONG JIAN PING
    高分子ゲル研究討論会講演要旨集, 15 Jan. 2014, English
  • Reversible and Strong Adhesion of Polyampholyte Hydrogel　　　　　　　　　　　　　　　
    ROY CHANCHAL KUMAR, ABU BIN IHSAN, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子ゲル研究討論会講演要旨集, 15 Jan. 2014, English
  • Tetra‐PEGゲルを第一網目構造に持つDNゲルの物性評価　　　　　　　　　　　　　　　
    MATSUDA TAKAHIRO, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, NONOYAMA TAKAYUKI, SAKAI TAKAMASA, CHUNG UNG-IL, GONG JIAN PING
    高分子ゲル研究討論会講演要旨集, 15 Jan. 2014, Japanese
  • 繊維状高靱性ゲルの創製　　　　　　　　　　　　　　　
    HOSHINO KEN'ICHI, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    繊維学会予稿集, 2014, Japanese
  • Self-healing behavior of tough hydrogel created by dynamic and reversible sacrificial bonds　　　　　　　　　　　　　　　
    IHSAN ABU BIN, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会北海道支部研究発表会講演要旨集, 2014, English
  • Shear Adhesion of Novel Polyampholyte Hydrogel　　　　　　　　　　　　　　　
    ROY CHANCHAL KUMAR, OKAZAKI HARUKA, IHSAN ABU BIN, SUN TAO LIN, NONOYAMA TAKAYUKI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会北海道支部研究発表会講演要旨集, 2014, English
  • 均一な第一網目構造を持つ超高強度DNゲルの一軸伸長変形挙動解析　　　　　　　　　　　　　　　
    MATSUDA TAKAHIRO, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, NONOYAMA TAKAYUKI, SAKAI TAKAMASA, CHUNG UNG-IL, GONG JIAN PING
    エラストマー討論会講演要旨集, 10 Dec. 2013, Japanese
  • 生体硬組織にならう高靱性DNゲル中でのミネラリゼーション　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KIYAMA RYUJI, NAKAJIMA TASUKU, KUROKAWA TAKAYUKI, GONG JIAN PING
    高分子学会予稿集(CD-ROM), 28 Aug. 2013, Japanese
  • ペプチドを用いたバイオミネラリゼーションによる無機結晶の成長制御　　　　　　　　　　　　　　　
    ANDO MASAYUKI, NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI
    高分子学会予稿集(CD-ROM), 14 May 2013, Japanese
  • pHマルチレスポンス型ハイドロゲルの創成とDDSへの応用　　　　　　　　　　　　　　　
    ICHII SHO, NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI
    高分子学会予稿集(CD-ROM), 14 May 2013, Japanese
  • バイオミネラリゼーションにならう有機・無機界面におけるエピタキシャル性　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, SATO KIMIYASU, KATO KATSUYA
    高分子学会予稿集(CD-ROM), 05 Sep. 2012, Japanese
  • 骨充填剤に応用可能なリン酸カルシウム含有ペプチドゲルの創成　　　　　　　　　　　　　　　
    OGASAWARA HOKUTO, NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI
    高分子学会予稿集(CD-ROM), 05 Sep. 2012, Japanese
  • ペプチドの自己組織化を利用した薬物徐放制御システムの開発　　　　　　　　　　　　　　　
    MURAI KAZUKI, NONOYAMA TAKAYUKI, HIGUCHI MASAHIRO, KATO KATSUYA
    高分子学会予稿集(CD-ROM), 15 May 2012, Japanese
  • バイオミネラリゼーションによるペプチド単分子膜上でのリン酸カルシウムの結晶成長　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA KENJI, SATO KIMIYASU, KATO KATSUYA
    高分子学会予稿集(CD-ROM), 15 May 2012, Japanese
  • バイオミネラリゼーションにならう有機・無機界面におけるエピタキシャルな関係性
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA KENJI, SATO KIMIYASU, KATO KATSUYA
    日本セラミックス協会年会講演予稿集, 19 Mar. 2012, Japanese
    バイオミネラリゼーションにおいて、どの段階で何が無機物の結晶相やモルフォロジーを決定しているかは、結晶学的にも非常に興味深い。事実、骨形成においてハイドロキシアパタイト(HAp) がコラーゲンファイバーに沿って形成されるように、バイオミネラリゼーションプロセスは無機構造を自在に制御する可能性を秘めている。我々はこれまでに、β-シートペプチド単分子膜上でのリン酸カルシウムのミネラリゼーションを通して有機・無機界面におけるエピタキシャルな関係性を調査してきた。β-シートペプチドはアミノ酸側鎖の官能基が 2 次元平面で規則正しく配列している。 今回はHAp の a 面及び c 面のカルシウム原子の位置に対応するように、カルボキシル基を配置した二種類のペプチドを新規設計し、 リン酸カルシウムのミネラリゼーションにおいて、形成した結晶相や結晶方位と対応するかを検証した。
  • pH応答ペプチドを利用した薬物除放制御システムの開発　　　　　　　　　　　　　　　
    MURAI KAZUYOSHI, NONOYAMA TAKAYUKI, ANDO FUMIO, KATO KATSUYA
    日本セラミックス協会東海支部学術研究発表会講演要旨集, 03 Dec. 2011, Japanese
  • バイオミネラリゼーションにならうペプチド単分子膜上でのリン酸カルシウムの結晶成長　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA KENJI, SATO KIMIYASU, KATO KATSUYA
    日本セラミックス協会東海支部学術研究発表会講演要旨集, 03 Dec. 2011, Japanese
  • 単一シーケンスペプチドを用いたバイオミネラリゼーション挙動の解析　　　　　　　　　　　　　　　
    KUNO TATSUYA, NONOYAMA TAKAYUKI, HIRAO KIYOSHI, KATO KATSUYA
    日本セラミックス協会東海支部学術研究発表会講演要旨集, 03 Dec. 2011, Japanese
  • バイオミネラリゼーションにおけるチャージリレー効果　　　　　　　　　　　　　　　
    KUNO TATSUYA, NONOYAMA TAKAYUKI, HIRAO KIYOSHI, KATO KATSUYA
    中部化学関係学協会支部連合秋季大会講演予稿集, 05 Nov. 2011, Japanese
  • ペプチドテンプレートによる無機物の結晶相及びモルフォロジー制御　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA KENJI, SATO KIMIYASU, KATO KATSUYA
    中部化学関係学協会支部連合秋季大会講演予稿集, 05 Nov. 2011, Japanese
  • 表面機能化メソポーラスシリカ上へのスブチリシンの固定化と表面官能基が酵素触媒特性に与える影響　　　　　　　　　　　　　　　
    MURAI KAZUKI, NONOYAMA TAKAYUKI, ANDO FUMIO, KATO KATSUYA
    高分子学会予稿集(CD-ROM), 13 Sep. 2011, Japanese
  • ペプチドゲル中でのミネラリゼーションにおけるリン酸カルシウムの合成　　　　　　　　　　　　　　　
    OGASAWARA HOKUTO, NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI
    高分子学会予稿集(CD-ROM), 13 Sep. 2011, Japanese
  • Titanium Dioxide Biomineralization with Peptide Nanofiber　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA KENJI, SATO KIMIYASU, KATO KATSUYA
    高分子学会予稿集(CD-ROM), 13 Sep. 2011, English
  • 自己組織化ペプチドを用いたバイオミネラル形成
    KUNO TATSUYA, NONOYAMA TAKAYUKI, HIRAO KIYOSHI, KATO KATSUYA
    日本セラミックス協会秋季シンポジウム講演予稿集, 07 Sep. 2011, Japanese
    生物が合成し利用するバイオミネラルは、常温常圧の低環境負荷条件で合成され、かつ有機物と複合化することによりナノスケールで制御されている。そのため、このバイオミネラル形成（バイオミネラリゼーション）は近年、省エネと小型化の双方の観点から注目を集めている。このバイオミネラリゼーションの挙動をより深く理解するために、本研究では、二次構造を制御した自己組織化ペプチドを固相合成し、そのペプチドをバイオミネラリゼーションの触媒として利用し、その触媒活性と合成される無機材料の評価を行った。
  • ペプチドファインチューニング単分子膜表面におけるリン酸カルシウムの結晶相とモルフォロジーの制御
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MAYUMI, NAGATA KENJI, SATO KIMIYASU, KATO KATSUYA
    日本セラミックス協会秋季シンポジウム講演予稿集, 07 Sep. 2011, Japanese
    有機無機界面において、一方の表面の化学的性質や形状は、他方のそれに大きく影響を与える。つまり表面を高度に設計することで他方の物質を制御できる。今回は、高度に配向秩序化された有機物表面が無機物の結晶相とモルフォロジーに与える影響を調査した。実際にはリン酸カルシウムのミネラリゼーションをシーケンシャルペプチド自己組織化単分子膜上で行いその析出物を透過電子顕微鏡や三次元断層影像法を用いて解析した。今回の基礎的知見は有機無機ハイブリッド材料創成における一つの指針となるかもしれない。
  • バイオミネラリゼーションにおけるペプチドの官能基と配列の効果
    KUNO TATSUYA, NONOYAMA TAKAYUKI, HIRAO KIYOSHI, KATO KATSUYA
    日本セラミックス協会年会講演予稿集, 16 Mar. 2011, Japanese
    Biominerals such as bone, tooth and shell, prepared under mild conditions, have high mechanical properties. Organic functional groups on the surface of organic matrices like protein, lipid and polysaccharide have an important role for the mineral formation in living body. In order to evaluate the organic functional groups for biomineralization, peptides with single sequence were prepared and their catalytic effects were investigated. In addition, structural properties of silica minerals synthesized with peptide were also evaluated.
  • 固相合成人工ペプチドをテンプレートとするバイオミネラリゼーション挙動の解析　　　　　　　　　　　　　　　
    KUNO TATSUYA, NONOYAMA TAKAYUKI, HIRAO KIYOSHI, KATO KATSUYA
    日本セラミックス協会東海支部学術研究発表会講演要旨集, 18 Dec. 2010, Japanese
  • ペプチドスキャフォールドを用いた官能基秩序界面上でのバイオミネラリゼーションによるリン酸カルシウムの結晶形態制御　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, SATO KIMIYASU, KATO KATSUYA
    日本セラミックス協会東海支部学術研究発表会講演要旨集, 18 Dec. 2010, Japanese
  • Peptide-assisted Preparation of Silica Nano Particles and Their Application for Biomolecule Encapsulation　　　　　　　　　　　　　　　
    KATO KATSUYA, NISHIDA MASAKAZU, NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI
    日本セラミックス協会秋季シンポジウム講演予稿集, 14 Nov. 2010, English
  • β‐シートペプチドから成る官能基二次元配向膜上でのミネラリゼーションによるリン酸カルシウムの合成　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA FUKUE, SATO KIMIYASU, KATO KATSUYA
    中部化学関係学協会支部連合秋季大会講演予稿集, 06 Nov. 2010, Japanese
  • 官能基秩序配列を有したペプチドスキャフォールド上でのミネラリゼーション制御　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA FUKUE, SATO KIMIYASU, KATO KATSUYA
    日本化学会バイオテクノロジー部会シンポジウム講演要旨集, 24 Sep. 2010, Japanese
  • 官能基秩序配列を有したペプチドスキャフォールド上でのミネラリゼーションによるリン酸カルシウムの合成　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI, HIGUCHI MASAHIRO, NAGATA FUKUE, SATO KIMIYASU, KATO KATSUYA
    高分子学会予稿集(CD-ROM), 01 Sep. 2010, Japanese
  • ペプチドテンプレート上でのミネラリゼーションによるリン酸カルシウム合成　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, NAGATA FUKUE, SATO KIMIYASU, KATO KATSUYA
    高分子学会予稿集(CD-ROM), 11 May 2010, Japanese
  • ペプチドテンプレート上でのミネラリゼーションによるリン酸カルシウム合成　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, KINOSHITA TAKATOSHI, NAGATA FUKUE, SATO KIMIYASU, KATO KATSUYA
    日本セラミックス協会年会講演予稿集, 22 Mar. 2010, Japanese
  • Mineralization on Peptide template
    Nonoyama Takayuki, Nagata Fukue, Sato Kimiyasu, Kato Katsuya, Kinoshita Takatoshi
    Preprints of Annual Meeting of The Ceramic Society of Japan
    Preprints of Fall Meeting of The Ceramic Society of Japan, 2010, Japanese
    有機鎖官能基が空間的に厳密に配置されたペプチドテンプレートを用いて、リン酸カルシウムのミネラリゼーションを行った。β-シート型ペプチド(LE)8-PEG70はグルタミン酸(E)とロイシン(L)の交互シークエンスにより設計し浸漬法により単分子膜を調製した。この基板上に酢酸カルシウム水溶液とリン酸水素二アンモニウム水溶液を交互に滴下しリン酸カルシウムを析出させた。AFM測定により、ペプチドによる等間隔のファイバー構造が観察された。溶液濃度Ca 50 mM, P 30 mMのとき、高配向のナノレーン状析出物がSEM測定より確認された。XPS、TEMおよびED測定からリン酸カルシウムの析出物はアモルファスであることが明らかになった。さらに析出の初期段階において析出形態の違いが確認された。
  • 核酸塩基対を利用したβ‐シートペプチド自己組織化膜上への金ナノ粒子の固定化と配列制御　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, HIGUCHI MASAHIRO, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI
    高分子学会予稿集(CD-ROM), 01 Sep. 2009, Japanese
  • ペプチドファイバーゲルによるアルブミンの認識　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, SAITO AI, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI
    繊維学会夏季セミナー講演要旨集, 27 Aug. 2009, Japanese
  • ペプチド自己組織化ナノファイバー上への金ナノ粒子の選択的固定化　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, HIGUCHI MASAHIRO, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI
    繊維学会夏季セミナー講演要旨集, 27 Aug. 2009, Japanese
  • 相補的水素結合を利用したβ‐シートペプチド自己組織化膜上への金ナノ粒子の固定化　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI
    高分子学会予稿集(CD-ROM), 12 May 2009, Japanese
  • 相補的水素結合を利用したβ‐シートペプチド自己組織化膜上への金ナノ粒子の固定化　　　　　　　　　　　　　　　
    NONOYAMA TAKAYUKI, TANAKA MASAYOSHI, KINOSHITA TAKATOSHI
    高分子学会予稿集(CD-ROM), 09 Sep. 2008, Japanese

Courses

  • Physics　　　　　　　　　　　　　　　
    Hokkaido university
  • 物理学I　　　　　　　　　　　　　　　
    北海道大学

Affiliated academic society

  • THE CHEMICAL SOCIETY OF JAPAN　　　　　　　　　　　　　　　
  • THE CERAMIC SOCIETY OF JAPAN　　　　　　　　　　　　　　　
  • THE SOCIETY OF POLYMER SCIENCE, JAPAN　　　　　　　　　　　　　　　

Research Themes

  • Design of Low Entropy Polymer Network Materials for Unique Functions
    Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (International Leading Research )
    20 Dec. 2022 - 31 Mar. 2029
    グン 剣萍, Li Xiang, 野々山 貴行, 中島 祐
    Japan Society for the Promotion of Science, Fund for the Promotion of Joint International Research (International Leading Research ), Hokkaido University, 22K21342
  • Metabolic Material System: Material Growth by Metabolic-like Destruction and Remodeling
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S)
    27 Apr. 2022 - 31 Mar. 2027
    グン 剣萍, 中島 祐, 印出井 努, 野々山 貴行
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (S), Hokkaido University, 22H04968
  • 工業リグニンの構造－物性相関の解明と高機能材料化技術の創出　　　　　　　　　　　　　　　
    ALCA-Next
    Nov. 2023 - Mar. 2027
    鈴木 栞, 野々山 貴行, 徳永 有希
    国立研究開発法人 科学技術振興機構, 北海道大学, JPMJAN23C3
  • 生物に習う高温でガラス化する高分子材料の創製とその学理解明　　　　　　　　　　　　　　　
    Fusion Oriented REsearch for disruptive Science and Technology (FOREST)
    Apr. 2021 - Mar. 2027
    Japan Science and Technology Agency, Hokkaido University, Principal investigator
  • Elucidation of extreme phase separation formation and rubbery-to-glassy transition with elevating temperature
    Grants-in-Aid for Scientific Research
    01 Apr. 2021 - 31 Mar. 2026
    野々山 貴行
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 21H01990
  • Establishment of a framework for self-evolving materials through mechanical training
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    01 Apr. 2022 - 31 Mar. 2025
    グン 剣萍, 印出井 努, 野々山 貴行, キング ダニエル, 中島 祐
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 22H00327
  • 高強度ポーラスゲルによる３次元がん組織モデル創出とがん幹細胞標的新規治療法の開発
    科学研究費助成事業 基盤研究(B)
    01 Apr. 2021 - 31 Mar. 2024
    津田 真寿美, 野々山 貴行, 田中 伸哉
    がんの再発は、がん治療に耐性を示すがん幹細胞が生き残ることによって起きる。がんの根絶には、がん組織におけるがん幹細胞の誕生・生存機構の解明が必須であるが、がん幹細胞が微量なことに加えて、従来のポリスチレンdish上でのがん細胞単独培養法は生体内のがん組織環境と大きく乖離しており、真の解明に至っていない。研究代表者らは、近年、高強度ダブルネットワークゲル（DNゲル）上にがん細胞を播種すると、24時間以内に迅速にがん幹細胞が誘導されるリプログラミング（初期化）現象を見出した（Nat. Biomed. Eng., 2021）。当該技術を基盤として、本研究ではがん組織におけるがん幹細胞の誕生・生存機序の解明を目指す。
    令和3年度は、高頻度に再発を引き起こす肉腫の生体内環境を模倣するために、高強度DNゲルの作製技術と多孔質ゲルを得る凍結重合法を組み合わせて、高強度多孔質（ポーラス）ゲルを作製した。ポーラスゲルは、1st モノマーにアニオン性高分子NaAMPSを用い、2ndモノマーに中性高分子AAmを用いた。筋組織を模倣するため、細胞外基質としてフィブロネクチンを吸着後、GFPラベル筋芽細胞を播種し、細胞の接着や長期生存を検討した。AAmの濃度を変えることでポーラスサイズを調整し、さらに細胞の播種方法を改善することにより、ポーラスゲルの深部まで細胞が進展するよう3次元組織モデルを最適化した。このポーラスゲルをマウスの筋肉内に埋植すると、１週間以内に宿主細胞がゲル内に侵入して組織を復元した。一方、氷の成長方向を制御するフリーズキャスティング法を用いた凍結重合法により、筋組織を模倣した高度な配向構造を有するポーラスゲルの創製に成功した。
    日本学術振興会, 基盤研究(B), 北海道大学, 21H03802
  • Utilizing the Sacrificial Bonding Principle to Create Soft-Hard Composites with Toughness that Surpasses Metals and Novel Functions
    Grant-in-Aid for Scientific Research (S)
    May 2017 - Mar. 2022
    Jian Ping Gong
    ハード相を犠牲結合とする複合材料では、プラスチックの三次元格子によるハード相と水中における膨潤能を有するハイドロゲルによるソフト相を用い、ソフト相とハード相のポアソン比ミスマッチが複合材料の膨潤・力学特性に与える影響を解析した。ハイドロゲルは、溶媒に浸漬させると溶媒を取り込んで膨潤（体積変化）する性質を持つ。ハード相にジャングルジム型構造を採用した複合材料を溶媒に浸漬させた場合、そのジャングルジム型構造がソフト相の膨潤に全く追随出来ないため、ソフト相の膨潤に伴ってハード相の構造が崩壊し、弾性率・強度が大きく損なわれる。一方、ハード相に負のポアソン比を持つオーセチック構造を採用した場合、ソフト相の膨潤収縮にハード相が追随可能であるため、複合材料が膨潤または収縮しても構造崩壊が起こらず、優れた力学特性が保たれることが見いだされた。
    続いてソフト相を犠牲結合とする複合材料では、ソフト相の局所的大変形による粘弾性効果が、ソフト複合材料を大きく強靭化していることを見出した。複合材料の幅が狭い場合、その亀裂進行は、ソフト相からハード相（繊維）が引き抜かれることに起因するソフト相の破断により生じることが見いだされた。ここで、繊維引き抜きが起こる部位ではソフト相が局所的に高速で大変形し、粘性的なエネルギー散逸が生じることで、材料の引裂エネルギーが極めて高くなると予想された。我々は、この局所的な粘性散逸の効果を表す半経験的な式を導いた。本式に従い、ソフト相単独の機械的特性とハード相の構造因子を最適化することで、粘性散逸の効果を増大させ、本タイプのソフト複合材料の靭性をさらに高めることに成功した。
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (S), Hokkaido University, Competitive research funding, 17H06144
  • Fabrication of Soft Photonic Crystals for Novel Functions　　　　　　　　　　　　　　　
    Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    Jun. 2017 - Feb. 2022
    Jian Ping Gong
    Japan Society for the Promotion of Science, Competitive research funding
  • Isochoric Hydrogel Possessing Thermal Toughening　　　　　　　　　　　　　　　
    Grant-in-Aid for Challenging Research (Exploratory)
    Jun. 2017 - Mar. 2020
    nonoyamatakayuki
    Japan Society for the Promotion of Science, Principal investigator, Competitive research funding
  • Creation of soft/hard complex materials tougher than metal based on the
    Grants-in-Aid for Scientific Research
    01 Apr. 2017 - 31 Mar. 2018
    グン 剣萍, 黒川 孝幸, 中島 祐, 野々山 貴行, キング ダニエル
    本研究では、高靭性ゲルの力学挙動（引張試験で見られる降伏現象、弾性率の低下を伴うヒステリシス現象、および引裂試験で見られる亀裂周辺の降伏領域(内部破壊領域)の形成）の三つの特徴を評価することによって、ゲルの靭性及びその高靭性化機構を解明することを目的とした。研究機関内には、ポリアンフォライトゲル/グラスファイバー織物の複合体について、様々な幅のサンプルを作製して引裂試験を行い、破壊エネルギーと降伏領域の関係を見積もった。サンプルの幅が降伏領域のサイズ（~cm）より小さい場合、複合体の破壊エネルギーはサンプルの幅と正の相関があった。一方、サンプルの幅が降伏領域より大きい場合、複合体の破壊エネルギーはサンプルの幅に依存しなかった。これは、本複合体の異常に高い破壊エネルギーが、亀裂近傍の降伏領域形成によるエネルギー散逸によることを強く示唆する結果である。また、破壊されたサンプルを走査型電子顕微鏡で観察した結果、繊維近傍におけるゲル層の破壊が確認された。ここから、本複合体の変形時には、ゲル-繊維間の応力伝達により、ゲル層の変形と破壊によるエネルギー散逸が高効率で発生し、それが本複合体を高効率にタフ化していることが示唆された。
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 17H01222
  • Proof of the Universality of Sacrificial Bond Principle and Creation of Tough Functional Hydrogels with Variety of Sacrificial Bonds
    Grants-in-Aid for Scientific Research
    31 May 2012 - 31 Mar. 2017
    Gong Jian Ping, NONOYAMA Takayuki, SUN Taolin
    Gels have been anticipated as tissue-like functional materials, but generally they are mechanically too weak to be applied widely. On the other hand, recently our group
    has proposed "sacrificial bond principle", where toughness of gels can be effectively improved by introducing weak and brittle bonds into soft and stretchable networks. In this project, we have created a series of extremely tough gels using this principle, e.g., variants of double network gels
    featuring covalent sacrificial bonds, polyampholyte gels containing ionic sacrificial bonds, and phase-separated polyacrylamide gels exhibiting super toughness based on solvophobic sacrificial bonds. We have also succeeded in adding functions corresponding to chemical species of sacrificial bonds to the tough gels, e.g., self-healing properties to polyampholyte gels and bioactivity to double network gels containing biopolymers. Through these studies, we have confirmed generality and diversity of sacrificial bond principle.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (S), Hokkaido University, 24225006
  • 高強度ハイドロゲル中でのリン酸カルシウムのバイオミネラリゼーション　　　　　　　　　　　　　　　
    Grants-in-Aid for Scientific Research
    Apr. 2014 - Mar. 2017
    Takayuki Nonoyama
    Japan Society for the Promotion of Science, Principal investigator, Competitive research funding