Jan. 2016, 日本分析化学会北海道支部, 北海道分析化学賞　　　　　　　　　　　　　　　
簡易分析のためのバイオ・化学システムおよびマイクロ流体システムの構築
石田晃彦

Noncompetitive Fluorescence Polarization Aptamer Assay for Detecting SARS-CoV-2 Variants
Chang Yu-Ting, Rigel Suzuki, Yoichiro Fujioka, Akihiko Ishida, Ayuko Imai, Mao Fukuyama, Motohiro Kasuya, Yusuke Ohba, Takasuke Fukuhara, Koji Shigemura, Akihide Hibara, Masatoshi Maeki, Manabu Tokeshi
ACS Omega, 24 Oct. 2025
Scientific journal

Isolating and Determining the Structures of Colored Products from the Reactions of Cannabinoids with Fast Blue RR
Kayo Nakamura, Hikari Nishiguchi, Ryosuke Arai, Riho Hamajima, Hiroko Abe, Akihiko Ishida, Manabu Tokeshi, Kyohei Higashi, Akiyoshi Saitoh, Hideyo Takahashi
Molecules, 22 Aug. 2025
Scientific journal

Paper discs in a 3D printed microplate hybrid microfluidic device for low-cost, rapid, and ultrasensitive paper-based bioluminescence detection of human epidermal growth factor receptor 2 (HER2) breast cancer biomarker
Ahmed A. Shalaby, Asmaa Salah, Akihiko Ishida, Masatoshi Maeki, Manabu Tokeshi
Biosensors and Bioelectronics: X, Aug. 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Microfluidic Production of Exosome-Mimicking Lipid Nanoparticles for Enhanced RNA Delivery: Role of Exosomal Proteins
Masatoshi Maeki, Ayuka Niwa, Shota Oyama, Kyoko Aratani, Rina Ito, Yuichi Suzuki, Yusuke Sato, Akihiko Ishida, Hideyoshi Harashima, Manabu Tokeshi
ACS Applied Materials & Interfaces, 23 Jul. 2025, [Peer-reviewed]
Scientific journal

Microfluidic technologies for protein crystallography: advances and applications.
Masatoshi Maeki, Akihiko Ishida, Manabu Tokeshi
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 21 Apr. 2025, [Peer-reviewed], [International Magazine]
English, Scientific journal, Three-dimensional protein structure determination by X-ray crystallography is essential for understanding biological function and accelerating drug discovery. However, obtaining high-quality protein crystals remains a significant bottleneck. The conventional crystallization methods are often labor-intensive, require large sample volumes, and offer limited control over the crystallization environment. This review summarizes the application of microfluidic technologies to protein crystallography with a focus on their advantages over the conventional crystallization methods. Microfluidic devices enable nanoliter-scale sample handling, precise control over crystallization conditions, and high-throughput screening, addressing major limitations of the conventional approaches. This review introduces various microfluidic platforms, including droplet-based and microwell-based systems, for protein crystallization, crystal growth control, and on-chip X-ray diffraction analysis. The review also covers the use of microfluidics for creating diffusion-controlled crystal growth environments, real-time crystal growth measurement, on-chip X-ray diffraction measurement, and room-temperature X-ray crystallography with automated data processing.

2-Propanol Suspension Method to Increase Acetylcholinesterase and Flow Stability on μPADs
Akinori Yamaguchi, Shota Oyama, Akihiko Ishida, Takanori Enomoto, Nobuyuki Sanari, Hajime Miyaguchi, Manabu Tokeshi
ACS Applied Bio Materials, 17 Feb. 2025, [Peer-reviewed]
English, Scientific journal

Straightforward high-temperature antibody immobilization for rapid and simple microfluidic paper-based ELISA for detection of β’-component (Onc k 5) protein, a major IgE-binding protein in salmon roe
Ahmed A. Shalaby, Ryoga Maeda, Akihiko Ishida, Yutaka Shimizu, Hiroki Saeki, Masatoshi Maeki, Manabu Tokeshi
Sensors and Actuators B: Chemical, 422, 01 Jan. 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Development of Polymer-Lipid Hybrid Nanoparticles for Large-Sized Plasmid DNA Transfection.
Masatoshi Maeki, Shuya Uno, Kaisei Sugiura, Yusuke Sato, Yoichiro Fujioka, Akihiko Ishida, Yusuke Ohba, Hideyoshi Harashima, Manabu Tokeshi
ACS applied materials & interfaces, 16, 2, 2110, 2119, 17 Jan. 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, RNA and DNA delivery technologies using lipid nanoparticles (LNPs) have advanced significantly, as demonstrated by their successful application in mRNA vaccines. To date, commercially available RNA therapeutics include Onpattro, a 21 bp siRNA, and mRNA vaccines comprising 4300 nucleotides for COVID-19. However, a significant challenge remains in achieving efficient transfection, as the size of the delivered RNA and DNA increases. In contrast to RNA transfection, plasmid DNA (pDNA) transfection requires multiple steps, including cellular uptake, endosomal escape, nuclear translocation, transcription, and translation. The low transfection efficiency of large pDNA is a critical limitation in the development of artificial cells and their cellular functionalization. Here, we introduce polymer-lipid hybrid nanoparticles designed for efficient, large-sized pDNA transfection. We demonstrated that LNPs loaded with positively charged pDNA-polycation core nanoparticles exhibited a 4-fold increase in transfection efficiency for 15 kbp pDNA compared with conventional LNPs, which encapsulate a negatively charged pDNA-polycation core. Based on assessments of the size and internal structure of the polymer-lipid nanoparticles as well as hemolysis and cellular uptake analysis, we propose a strategy to enhance large-sized pDNA transfection using LNPs. This approach holds promise for accelerating the in vivo delivery of large-sized pDNA and advancing the development of artificial cells.

Understanding the effects of ethanol on the liposome bilayer structure using microfluidic-based time-resolved small-angle X-ray scattering and molecular dynamics simulations
Masatoshi Maeki, Niko Kimura, Yuto Okada, Kazuki Shimizu, Kana Shibata, Yusuke Miyazaki, Akihiko Ishida, Kento Yonezawa, Nobutaka Shimizu, Wataru Shinoda, Manabu Tokeshi
Nanoscale Advances, Royal Society of Chemistry (RSC), 2024, [Peer-reviewed]
Scientific journal, We investigated ethanol-induced structural changes in liposomes on a time scale from microseconds to tens of seconds using a microfluidic-based small-angle X-ray scattering (SAXS) measurement system coupled with molecular dynamics (MD) simulations.

A portable liquid chromatography system based on a separation/detection chip module consisting of a replaceable ultraviolet-visible absorbance or contactless conductivity detection unit
Akihiko Ishida, Takuma Nishimura, Kaito Koyama, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
Journal of Chromatography A, 1706, 464272, 464272, Elsevier BV, Sep. 2023, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
English, Scientific journal

Understanding the effects of ethanol on structural changes in liposomes using microfluidic-based time-resolved small-angle X-ray scattering and MD simulations
Masatoshi Maeki, Niko Kimura, Yuto Okada, Kazuki Shimizu, Kana Shibata, Yusuke Miyazaki, Akihiko Ishida, Kento Yonezawa, Nobutaka Shimizu, Wataru Shinoda, Manabu Tokeshi
American Chemical Society (ACS), 09 Jun. 2023, [Peer-reviewed]
Lipid nanoparticles (LNPs) are essential carrier particles in drug delivery systems, particularly in ribonucleic acid delivery. In preparing lipid-based nanoparticles, microfluidic-based ethanol injection may produce precisely size-controlled nanoparticles. Ethanol is critical in LNP formation and post-treatment processes and affects liposome size, structure, lamellarity, and drug-loading efficiency. However, the effects of time-dependent changes in the ethanol concentration on the structural dynamics of liposomes are not clearly understood. Herein, we investigated ethanol-induced structural changes in liposomes on a time scale from microseconds to tens of seconds using a microfluidic-based small-angle X-ray scattering (SAXS) measurement system coupled with molecular dynamics (MD) simulations. The time-resolved SAXS measurement system revealed that single unilamellar liposomes converted to multilamellar liposomes within 0.8 s of contact with ethanol, and the d-spacing was decreased from 6.1 (w/o ethanol) to 4.4 nm (80% ethanol) with increasing ethanol concentration. We conducted 1 µs MD simulations to understand the molecular-level structural changes in the liposomes. The MD simulations revealed that the changes in the lamellar structure caused by ethanol at the molecular level could explain the structural changes in the liposomes observed via time-resolved SAXS. Therefore, the post-treatment process to remove residual ethanol is critical in liposome formation.

Mass production system for RNA-loaded lipid nanoparticles using piling up microfluidic devices
Masatoshi Maeki, Yuto Okada, Shuya Uno, Kaisei Sugiura, Yuichi Suzuki, Kento Okuda, Yusuke Sato, Masao Ando, Hiroyuki Yamazaki, Masaki Takeuchi, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
Applied Materials Today, Apr. 2023, [Peer-reviewed]
Scientific journal

Microfluidic paper-based analytical devices for cancer diagnosis
Ahmed A. Shalaby, Chia Wen Tsao, Akihiko Ishida, Masatoshi Maeki, Manabu Tokeshi
Sensors and Actuators B: Chemical, 379, 15 Mar. 2023, [Peer-reviewed]
Scientific journal

Effects of Addition of Blocking Agents on Fluorescence Polarization Immunoassay of Okadaic Acid Using a Microfluidic Device
Shunsuke CHIDA, Kazuki TAKAHASHI, Mao FUKUYAMA, Motohiro KASUYA, Masatoshi MAEKI, Akihiko ISHIDA, Hirofumi TANI, Koji SHIGEMURA, Anatoly V. ZHERDEV, Sergei A. EREMIN, Akihide HIBARA, Manabu TOKESHI
BUNSEKI KAGAKU, 72, 3, 133, 138, Japan Society for Analytical Chemistry, 05 Mar. 2023, [Peer-reviewed]
Japanese, Scientific journal

Development of an absorbance detection module using a deep UV light-emitting diode for a portable miniaturized liquid chromatograph
Kaito Koyama, Takuma Nishimura, Akihiko Ishida, Mitsue Hibino, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
BUNSEKI KAGAKU, 72, 3, 125, 131, Mar. 2023, [Peer-reviewed], [Corresponding author]
Japanese, Scientific journal

A fabrication technique for paper-based analytical devices via two-sided patterning with thermal-transfer printer and laminator
Takuya Monju, Manabu Hirakawa, Satoshi Kuboyama, Rikuro Saiki, Akihiko Ishida
Sensors and Actuators B: Chemical, 375, 132886, 132886, Elsevier BV, Jan. 2023, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Effect of Organic Solvents on a Production of PLGA-Based Drug-Loaded Nanoparticles Using a Microfluidic Device
Yi Bao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Omega, 7, 37, 33079, 33086, 20 Sep. 2022, [Peer-reviewed]
Scientific journal

Preparation of size-tunable sub-200 nm PLGA-based nanoparticles with a wide size range using a microfluidic platform
Yi Bao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
PLOS ONE, 17, 8, Aug. 2022, [Peer-reviewed]
English, Scientific journal

Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer.
Kazuki Takahashi, Shunsuke Chida, Thanawat Suwatthanarak, Mikiko Iida, Min Zhang, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Takao Yasui, Yoshinobu Baba, Akihide Hibara, Mina Okochi, Manabu Tokeshi
Lab on a chip, 22, 16, 2971, 2977, 17 Jun. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
Masatoshi Maeki, Yuto Okada, Shuya Uno, Ayuka Niwa, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Journal of Visualized Experiments, 2022, 181, Mar. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal

Electrochemical enzyme-based blood ATP and lactate sensor for a rapid and straightforward evaluation of illness severity
Keine Nishiyama, Ryohei Mizukami, Shizuka Kuki, Akihiko Ishida, Junji Chida, Hiroshi Kido, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
Biosensors and Bioelectronics, 113832, 113832, Elsevier {BV}, Feb. 2022, [Peer-reviewed], [Corresponding author]
Scientific journal

Facile and rapid detection of SARS-CoV-2 antibody based on a noncompetitive fluorescence polarization immunoassay in human serum samples.
Keine Nishiyama, Kazuki Takahashi, Mao Fukuyama, Motohiro Kasuya, Ayuko Imai, Takumi Usukura, Nako Maishi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Kyoko Hida, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Biosensors & bioelectronics, 190, 113414, 113414, 15 Oct. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Antibody detection methods for viral infections have received broad attention due to the COVID-19 pandemic. In addition, there remains an ever-increasing need to quantitatively evaluate the immune response to develop vaccines and treatments for COVID-19. Here, we report an analytical method for the rapid and quantitative detection of SARS-CoV-2 antibody in human serum by fluorescence polarization immunoassay (FPIA). A recombinant SARS-CoV-2 receptor binding domain (RBD) protein labeled with HiLyte Fluor 647 (F-RBD) was prepared and used for FPIA. When the anti-RBD antibody in human serum binds to F-RBD, the degree of polarization (P) increases by suppressing the rotational diffusion of F-RBD. The measurement procedure required only mixing a reagent containing F-RBD with serum sample and measuring the P value with a portable fluorescence polarization analyzer after 15 min incubation. We evaluated analytical performance of the developed FPIA system using 30 samples: 20 COVID-19 positive sera and 10 negative sera. The receiver operating characteristic curve drawn with the obtained results showed that this FPIA system had high accuracy for discriminating COVID-19 positive or negative serum (AUC = 0.965). The total measurement time was about 20 min, and the serum volume required for measurement was 0.25 μL. Therefore, we successfully developed the FPIA system that enables rapid and easy quantification of SARS-CoV-2 antibody. It is believed that our FPIA system will facilitate rapid on-site identification of infected persons and deepen understanding of the immune response to COVID-19.

Determination of Deoxynivalenol in Wheat, Barley, Corn Meal, and Wheat-Based Products by Simultaneous Multisample Fluorescence Polarization Immunoassay Using a Portable Analyzer
Ayano Nakamura, Mitsutoshi Aoyagi, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
ACS Food Science & Technology, 1, 9, 1623, 1628, American Chemical Society ({ACS}), 15 Oct. 2021, [Peer-reviewed]
Scientific journal

Using a Paper-Based Analytical Device Designed for Remote Learning Environments to Achieve Simple Quantitative Colorimetry without Micropipettes
Takeshi Komatsu, Ryan Russel Gabatino, Harrienica Hofileña, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Journal of Chemical Education, 98, 9, 3050, 3054, American Chemical Society ({ACS}), 14 Sep. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Paper-Based Analytical Device for the On-Site Detection of Nerve Agents
Akinori Yamaguchi, Hajime Miyaguchi, Akihiko Ishida, Manabu Tokeshi
ACS Applied Bio Materials, 4, 8, 6512, 6518, American Chemical Society ({ACS}), 16 Aug. 2021, [Peer-reviewed]
Scientific journal

Simple Approach for Fluorescence Signal Amplification Utilizing a Poly(vinyl alcohol)-Based Polymer Structure in a Microchannel
Keine Nishiyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Hideaki Hisamoto, Manabu Tokeshi
ACS Omega, 6, 12, 8340, 8345, American Chemical Society ({ACS}), 30 Mar. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Analytical methods with fluorescence detection are in widespread use for detecting low abundance analytes. Here, we report a simple method for fluorescence signal amplification utilizing a structure of an azide-unit pendant water-soluble photopolymer (AWP) in a microchannel. The AWP is a poly(vinyl alcohol)-based photocross-linkable polymer, which is often used in biosensors. We determined that the wall-like structure of the AWP (AWP-wall) constructed in a microchannel functioned as an amplifier of a fluorescence signal. When a solution of fluorescent molecules was introduced into the microchannel having the AWP-wall, the fluorescent molecules accumulated inside the AWP-wall by diffusion. Consequently, the fluorescence intensity inside the AWP-wall increased locally. Among the fluorescent molecules considered in this paper, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) (DDAO) showed the highest efficiency of fluorescence signal amplification. We prepared a calibration curve for DDAO using the fluorescence intensity inside the AWP-wall, and the sensitivity was 5-fold that for the microchannel without the AWP-wall. This method realizes the improved sensitivity of fluorescence detection easily because the fluorescence signal was amplified only by injecting the solution into the microchannel having the AWP-wall. Furthermore, since this method is not limited to only the use of microchannel, we expect it to be applicable in various fields.

Dip-Type Paper-Based Analytical Device for Straightforward Quantitative Detection without Precise Sample Introduction
Takeshi Komatsu, Ryoga Maeda, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Sensors, 6, 3, 1094, 1102, American Chemical Society ({ACS}), 26 Mar. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, The development of low-cost, user-friendly paper-based analytical devices (PADs) that can easily measure target chemicals is attracting attention. However, most PADs require manipulation of the sample using sophisticated micropipettes for quantitative analyses, which restricts their user-friendliness. In addition, immobilization of detection molecules to cellulose fibers is essential for achieving good measuring ability as it ensures the homogeneity of color development. Here, we have described a dip-type PAD that does not require pipette manipulation for sample introduction and immobilization of detection molecules to cellulose fibers and its application to ascorbic acid (AA) and pH assays. The PAD consisted of a dipping area and two channels, each with two detection zones. The developed PADs show color distribution in the two detection zones depending on the sample flow from the dipping area. In comparison with a PAD that has one detection zone at the end of the channel, our developed device achieved higher sensitivity (limit of detection (LOD), 0.22 mg/mL) and reproducibility (maximum coefficient of variation (CV), 2.4%) in AA detection. However, in pH detection, the reproducibility of the PAD with one detection zone at the end of the channel (maximum CV, 21%) was worse than that with two zones (maximum CV, 11%). Furthermore, a dipping time over 3 s did not affect color formation or calibration curves in AA detection: LODs at 3 and 30 s dipping time were 18 and 5.8 μg/mL, respectively. The simultaneous determination of AA and pH in various beverages was performed with no significant difference compared to results of the conventional method.

One-Step Production Using a Microfluidic Device of Highly Biocompatible Size-Controlled Noncationic Exosome-like Nanoparticles for RNA Delivery
Niko Kimura, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Applied Bio Materials, 4, 2, 1783, 1793, 15 Feb. 2021
English, Scientific journal

Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer.
Keine Nishiyama, Yohei Takeda, Kazuki Takahashi, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Haruko Ogawa, Manabu Tokeshi
Analytical and bioanalytical chemistry, 413, 18, 4619, 4623, 05 Feb. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Nowadays, the diagnosis of viral infections is receiving broad attention. We have developed a non-competitive fluorescence polarization immunoassay (NC-FPIA), which is a separation-free immunoassay, for a virus detection. H5 subtype avian influenza virus (H5-AIV) was used as a model virus for the proof of concept. The fluorescein-labeled Fab fragment that binds to H5 hemagglutinin was used for NC-FPIA. The purified H5-AIV which has H5 hemagglutinin was mixed with the fluorescein-labeled Fab fragment. After that, the degree of fluorescence polarization was measured with a portable FPIA analyzer. H5-AIV was successfully detected with an incubation time of 15 min. In addition, the portable FPIA analyzer enables performance of on-site NC-FPIA with a sample volume of 20 μL or less. This is the first research of detecting a virus particle by FPIA. This NC-FPIA can be applied to rapid on-site diagnosis of various viruses.

Rapid, sensitive universal paper-based device enhances competitive immunoassays of small molecules
Takeshi Komatsu, Yuki Sato, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analytica Chimica Acta, 1144, 85, 95, Elsevier BV, Feb. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Competitive immunoassays comprise the standard means of detecting small molecules. However, conventional methods using microwells are difficult to apply during point-of-care tests (POCT) because they require complicated handling and are time consuming. Although paper-based analytical devices (PAD) have received considerable focus because of their rapid and straightforward operation, only a few devices have been proposed for competitive immunoassays. Herein, we describe a novel universal PAD format with a 3-dimensional configuration for competitive immunoassays that rapidly and sensitively detects small molecules. The proposed device comprised a layered structure with uniform color formation and high capture efficiency between antigen and antibody that results in rapid and reproducible results. The device rapidly (90 s) assayed biotin as a model target, with a limit of detection (LOD) of 5.08 ng mL-1, and detected progesterone with an LOD of 84 pg mL-1 within 5 min. Moreover, sample volumes and reagent consumption rates were minimized. Thus, our device could be applied to competitive immunoassays of various small molecules in POCT.

Three-dimensional, symmetrically assembled microfluidic device for lipid nanoparticle production
Niko Kimura, Masatoshi Maeki, Kosuke Sasaki, Yusuke Sato, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
RSC Advances, 11, 3, 1430, 1439, 2021
English, Scientific journal

One-step non-competitive fluorescence polarization immunoassay based on a Fab fragment for C-reactive protein quantification
Keine Nishiyama, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi
Sensors and Actuators B: Chemical, 326, 128982, 128982, Elsevier {BV}, Jan. 2021
Scientific journal

Silica Nanopillar Arrays for Monitoring Diffraction-Based Label-Free Biomolecule Separation
Taiga Ajiri, Takao Yasui, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Junji Nishii, Yoshinobu Baba, Manabu Tokeshi
ACS Applied Nano Materials, 3, 9, 8810, 8816, 25 Sep. 2020
English, Scientific journal

Room-temperature crystallography using a microfluidic protein crystal array device and its application to protein–ligand complex structure analysis
Masatoshi Maeki, Sho Ito, Reo Takeda, Go Ueno, Akihiko Ishida, Hirofumi Tani, Masaki Yamamoto, Manabu Tokeshi
Chemical Science, 11, 34, 9072, 9087, Royal Society of Chemistry ({RSC}), 25 Aug. 2020, [International Magazine]
English, Scientific journal, Room-temperature (RT) protein crystallography provides significant information to elucidate protein function under physiological conditions. In particular, contrary to typical binding assays, X-ray crystal structure analysis of a protein-ligand complex can determine the three-dimensional (3D) configuration of its binding site. This allows the development of effective drugs by structure-based and fragment-based (FBDD) drug design. However, RT crystallography and RT crystallography-based protein-ligand complex analyses require the preparation and measurement of numerous crystals to avoid the X-ray radiation damage. Thus, for the application of RT crystallography to protein-ligand complex analysis, the simultaneous preparation of protein-ligand complex crystals and sequential X-ray diffraction measurement remain challenging. Here, we report an RT crystallography technique using a microfluidic protein crystal array device for protein-ligand complex structure analysis. We demonstrate the microfluidic sorting of protein crystals into microwells without any complicated procedures and apparatus, whereby the sorted protein crystals are fixed into microwells and sequentially measured to collect X-ray diffraction data. This is followed by automatic data processing to calculate the 3D protein structure. The microfluidic device allows the high-throughput preparation of the protein-ligand complex solely by the replacement of the microchannel content with the required ligand solution. We determined eight trypsin-ligand complex structures for the proof of concept experiment and found differences in the ligand coordination of the corresponding RT and conventional cryogenic structures. This methodology can be applied to easily obtain more natural structures. Moreover, drug development by FBDD could be more effective using the proposed methodology.

Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer
Keine Nishiyama, Yohei Takeda, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Yutaka Yonezawa, Kunitoshi Imai, Haruko Ogawa, Manabu Tokeshi
Sensors and Actuators B: Chemical, 316, 128160, 128160, Elsevier {BV}, Aug. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, A rapid, facile and selective detection of anti-H5 subtype avian influenza virus (AIV) antibody in serum by fluorescence polarization immunoassay (FPIA) was achieved. A fragment of recombinant H5 subtype AIVhemagglutinin was produced and labeled with fluorescein to use it as a labeled antigen in FPIA. This labeled antigen was mixed with anti-AIV sera (H1-H16 subtypes) and FP of the mixture was measured using a portable FP analyzer on a microdevice. It was found that FP increased in proportion to the concentration of anti-H5 AIV antibody (serum) and was significantly higher than FP obtained with the other sera. The selective detection of anti-H5 subtype AIV antibody was confirmed. The required volume of original sample was 2 μL and analysis time was within 20 min. This detection system realizes an efficient on-site diagnosis and surveillance of AIV.

Development of a Microfluidic-Based Post-Treatment Process for Size-Controlled Lipid Nanoparticles and Application to siRNA Delivery
Niko Kimura, Masatoshi Maeki, Yusuke Sato, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
ACS Applied Materials & Interfaces, 12, 30, 34011, 34020, American Chemical Society ({ACS}), 29 Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Microfluidic methodologies for preparation of lipid nanoparticles (LNPs) based on an organic solvent injection method enable precise size control of the LNPs. After preparation of LNPs, the organic solvent injection method needs some post-treatments, such as overnight dialysis or direct dilution with a buffer solution. LNP production using the microfluidic-based organic solvent injection method is dominated by kinetics rather than thermodynamics. Kinetics of ethanol removal from the inner and outer membranes of LNPs could induce a structural change in the membrane that could lead to fusion of LNPs. However, the effects of microfluidic post-treatment on the final size of LNPs have not been sufficiently understood. Herein, we investigated the effect of the post-treatment processes on the final product size of LNPs in detail. A simple baffle device and a model lipid system composed of a neutral phospholipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC) and cholesterol were used to produce the LNPs. We demonstrated that flow conditions of the post-treatment diluting the remaining ethanol in the LNP suspension affected the final product size of LNPs. Based on the findings, we developed an integrated baffle device composed of an LNP production region and a post-treatment region for a microfluidic-based LNP production system; this integrated baffle device prevented the undesirable aggregation or fusion of POPC LNPs even for the high-lipid-concentration condition. Finally, we applied our concept to small interfering RNA (siRNA) delivery and confirmed that no significant effects due to the continuous process occurred on the siRNA encapsulation efficiency, biological distribution, and knockdown activity. The microfluidic post-treatment method is expected to contribute to the production of LNPs for practical applications and the development of novel LNP-based nanomedicines.

Real-Time Measurement of Protein Crystal Growth Rates within the Microfluidic Device to Understand the Microspace Effect
Masatoshi Maeki, Shohei Yamazaki, Reo Takeda, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Omega, 5, 28, 17199, 17206, American Chemical Society ({ACS}), 21 Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Preparation of high-quality protein crystals is a major challenge in protein crystallography. Natural convection is considered to be an uncontrollable factor of the crystallization process at the ground level as it disturbs the concentration gradient around the growing crystal, resulting in lower-quality crystals. A microfluidic environment expects an imitated microgravity environment because of the small Gr number. However, the mechanism of protein crystal growth in the microfluidic device was not elucidated due to limitations in measuring the crystal growth process within the device. Here, we demonstrate the real-time measurement of protein crystal growth rates within the microfluidic devices by laser confocal microscopy with differential interference contrast microscopy (LCM-DIM) at the nanometer scale. We confirmed the normal growth rates in the 20 and 30 μm-deep microfluidic device to be 42.2 and 536 nm/min, respectively. In addition, the growth rate of crystals in the 20 μm-deep microfluidic device was almost the same as that reported in microgravity conditions. This phenomenon may enable the development of more accessible alternatives to the microgravity environment of the International Space Station.

Paper-Based Device for the Facile Colorimetric Determination of Lithium Ions in Human Whole Blood
Takeshi Komatsu, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Sensors, 5, 5, 1287, 1294, American Chemical Society ({ACS}), 22 May 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Lithium carbonate is an effective medicine for the treatment of the bipolar disorder, but the concentration of lithium in the patient's blood must be frequently monitored because of its toxicity. To date, no colorimetric methods of lithium ion detection in whole blood without pretreatment have been reported. Here, we report a colorimetric paper-based device that allows point-of-care testing in one step. This device is composed of two paper-based elements linked to each other: a blood cell separation unit and a colorimetric detection unit. After a portion of whole blood has been placed on the end of the separation unit, plasma in the sample is automatically transported to the detection unit, which displays a diagnostic color. The key feature of this device is its simple, user-friendly operation. The limit of detection is 0.054 mM and the coefficient of variance is below 6.1%, which are comparable to those of conventional instruments using the same colorimetric reaction. Furthermore, we achieved high recovery (>90%) and reproducibility (<9.8%) with spiked human blood samples. Thus, the presented device provides an alternative method for the regular monitoring of lithium concentrations in the treatment of bipolar disorder by augmenting the coefficient of variation (maximum value, 6.1%).

Development of a Paper-based Analytical Chip for the Detection of Bacterial 16S rRNA in Wastewater Samples
Meri Nakajima, Akihiko Ishida, Manabu Tokeshi, Hisashi Satoh
BUNSEKI KAGAKU, 69, 12, 715, 722, 2020
Japanese, Scientific journal

An aprotic polar solvent assisted size-tuning method for microfluidic production of lipid-based drug nanocarriers with various sizes
Niko Kimura, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1021, 1022, 2020
International conference proceedings

Novel format of a paper-based device for competitive immunoassays
Takeshi Komatsu, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 494, 495, 2020
International conference proceedings

Detection of avian influenza virus and its antibody by fluorescence polarization immunoassay
Keine Nishiyama, Yohei Takeda, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Haruko Ogawa, Manabu Tokeshi
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1226, 1227, 2020
International conference proceedings

An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A.
Mazaafrianto DN, Ishida A, Maeki M, Tani H, Tokeshi M
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 35, 11, 1221, 1226, Nov. 2019, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Rapid, Sensitive, and Selective Detection of H5 Hemagglutinin from Avian Influenza Virus Using an Immunowall Device
Kenia Chávez Ramos, Keine Nishiyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Toshihiro Kasama, Yoshinobu Baba, Manabu Tokeshi
ACS Omega, 4, 15, 16683, 16688, American Chemical Society ({ACS}), 08 Oct. 2019, [Peer-reviewed]
Scientific journal

Cover Picture: A Concentric Ring Electrode for a Wall‐jet Cell in a Microfluidic Device (Electroanalysis 9/2019)
Keine Nishiyama, Koki Hoshikawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Electroanalysis, 31, 9, 1736, 1743, 06 Sep. 2019, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Ultrasensitive detection of disease biomarkers using an immuno-wall device with enzymatic amplification
Keine Nishiyama, Toshihiro Kasama, Seiya Nakamata, Koya Ishikawa, Daisuke Onoshima, Hiroshi Yukawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi
Analyst, 144, 15, 4589, 4595, 07 Aug. 2019, [Peer-reviewed]
Scientific journal

Sensitive fluorescent polarization immunoassay by optimizing synchronization mismatch condition
Wakao Osamu, Maeki Masatoshi, Ishida Akihiko, Tani Hirofumi, Hibara Akihide, Tokeshi Manabu
SENSORS AND ACTUATORS B-CHEMICAL, 285, 418, 422, 15 Apr. 2019, [Peer-reviewed]
English, Scientific journal

High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer
Osamu Wakao, Ken Satou, Ayano Nakamura, Polina A. Galkina, Keine Nishiyama, Ken Sumiyoshi, Fumio Kurosawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Mikhail A. Proskurnin, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Lab on a Chip, 19, 15, 2581, 2588, 2019, [Peer-reviewed]
Scientific journal

High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer
Osamu Wakao, Ken Satou, Ayano Nakamura, Polina A. Galkina, Keine Nishiyama, Ken Sumiyoshi, Fumio Kurosawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Mikhail A. Proskurnin, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Lab on a Chip, 19, 15, 2581, 2588, Royal Society of Chemistry ({RSC}), 2019, [Peer-reviewed]
Scientific journal

Ultrasensitive detection of disease biomarkers using an immuno-wall device with enzymatic amplification
Keine Nishiyama, Toshihiro Kasama, Seiya Nakamata, Koya Ishikawa, Daisuke Onoshima, Hiroshi Yukawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi
The Analyst, 144, 15, 4589, 4595, Royal Society of Chemistry ({RSC}), 2019, [Peer-reviewed]
Scientific journal

Label-Free Electrochemical Sensor for Ochratoxin A Using a Microfabricated Electrode with Immobilized Aptamer
Donny N. Mazaafrianto, Akihiko Ishida, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
ACS Omega, 3, 12, 16823, 16830, 31 Dec. 2018, [Peer-reviewed], [Corresponding author]
English, Scientific journal

血中ATPと乳酸を指標とする重症度診断のための電気化学酵素センサーの開発　　　　　　　　　　　　　　　
水上 良平, 真栄城 正寿, 石田 晃彦, 谷 博文, 渡慶次 学
日本分析化学会講演要旨集, 67年会, 287, 287, (公社)日本分析化学会, Aug. 2018
Japanese

Development of the iLiNP Device: Fine Tuning the Lipid Nanoparticle Size within 10 nm for Drug Delivery
Niko Kimura, Masatoshi Maeki, Yusuke Sato, Yusuke Note, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
ACS Omega, 3, 5, 5044, 5051, 09 May 2018, [Peer-reviewed]
English, Scientific journal

Recent microdevice-based aptamer sensors
Donny Nugraha Mazaafrianto, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Micromachines, 9, 5, 25 Apr. 2018, [Peer-reviewed]
English

A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer
Osamu Wakao, Ken Satou, Ayano Nakamura, Ken Sumiyoshi, Masanori Shirokawa, Chikaaki Mizokuchi, Kunihiro Shiota, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Review of Scientific Instruments, 89, 2, 024103, 01 Feb. 2018, [Peer-reviewed]
English, Scientific journal

A post-treatment methodology for precise size control of lipid nanoparticles by stepwise and rapid ethanol dilution
Niko Kimura, Masatoshi Maeki, Nana Okabe, Yusuke Sato, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, 3, 1404, 1405, 2018
International conference proceedings

Characteristics of microfluidic paper-based analytical devices fabricated by four different methods
Takeshi Komatsu, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analytical Sciences, 34, 1, 39, 44, 2018, [Peer-reviewed]
English, Scientific journal

Understanding the formation mechanism of lipid nanoparticles in microfluidic devices with chaotic micromixers
Masatoshi Maeki, Yuka Fujishima, Yusuke Sato, Takao Yasui, Noritada Kaji, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Hideyoshi Harashima, Manabu Tokeshi
PLOS ONE, 12, 11, Nov. 2017, [Peer-reviewed]
English, Scientific journal

Optimization of the nanofluidic design for label-free detection of biomolecules using a nanowall array
Taiga Ajiri, Takao Yasui, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi
SENSORS AND ACTUATORS B-CHEMICAL, 250, 39, 43, Oct. 2017, [Peer-reviewed]
English, Scientific journal

Using Laser Interference Lithography in the Fabrication of a Simplified Micro and Nanofluidic Device for Label-free Detection
Taiga Ajiri, Haruya Kasa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Junji Nishi, Manabu Tokeshi
ANALYTICAL SCIENCES, 33, 10, 1197, 1199, Oct. 2017, [Peer-reviewed]
English, Scientific journal

尿中シュウ酸分析のための単一くし形電極を組み込んだ液体クロマトグラフィーチップの開発　　　　　　　　　　　　　　　
藤井 大地, 真栄城 正寿, 石田 晃彦, 谷 博文, 渡慶次 学
日本分析化学会講演要旨集, 66年会, 24, 24, (公社)日本分析化学会, Aug. 2017
Japanese

マイクロ流体デバイスを用いた化学発光イムノアッセイの高感度化　　　　　　　　　　　　　　　
菊地 優仁, 真栄城 正寿, 石田 晃彦, 谷 博文, 渡慶次 学
日本分析化学会講演要旨集, 66年会, 52, 52, (公社)日本分析化学会, Aug. 2017
Japanese

血中ATPと乳酸測定のための電気化学センサーの開発　　　　　　　　　　　　　　　
水上 良平, 西山 慶音, 真栄城 正寿, 石田 晃彦, 谷 博文, 渡慶次 学
日本分析化学会講演要旨集, 66年会, 134, 134, (公社)日本分析化学会, Aug. 2017
Japanese

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips
Saeed Mohammadi, Lori Shayne Alamo Busa, Masatoshi Maeki, Reza M. Mohamadi, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ANALYTICAL SCIENCES, 32, 12, 1359, 1362, Dec. 2016, [Peer-reviewed]
English, Scientific journal

Simple and sensitive colorimetric assay system for horseradish peroxidase using microfluidic paper-based devices
Lori Shayne Alamo Busa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
SENSORS AND ACTUATORS B-CHEMICAL, 236, 433, 441, Nov. 2016, [Peer-reviewed]
English, Scientific journal

Novel concept of washing for microfluidic paper-based analytical devices based on capillary force of paper substrates
Saeed Mohammadi, Lori Shayne Alamo Busa, Masatoshi Maeki, Reza M. Mohamadi, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 408, 27, 7559, 7563, Nov. 2016, [Peer-reviewed]
English, Scientific journal

血中リチウム濃度測定のためのペーパーデバイスの開発　　　　　　　　　　　　　　　
小松 雄士, 真栄城 正寿, 石田 晃彦, 谷 博文, 渡慶次 学
日本分析化学会講演要旨集, 65年会, 163, 163, (公社)日本分析化学会, Aug. 2016
Japanese

3,3 ',5,5 '-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays
Lori Shayne Alamo Busa, Takeshi Komatsu, Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ANALYTICAL SCIENCES, 32, 8, 815, 818, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Advances in Microfluidic Paper-Based Analytical Devices for Food and Water Analysis
Lori Shayne Alamo Busa, Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
MICROMACHINES, 7, 5, May 2016, [Peer-reviewed]
English

A microfluidic-based protein crystallization method in 10 micrometer-sized crystallization space
Masatoshi Maeki, Shohei Yamazaki, Ashtamurthy S. Pawate, Akihiko Ishida, Hirofumi Tani, Kenichi Yamashita, Masakazu Sugishima, Keiichi Watanabe, Manabu Tokeshi, Paul J. A. Kenis, Masaya Miyazaki
CRYSTENGCOMM, 18, 40, 7722, 7727, 2016, [Peer-reviewed]
English, Scientific journal

Image analysis for a microfluidic paper-based analytical device using the CIE L(star)a(star)b(star) color system
Takeshi Komatsu, Saeed Mohammadi, Lori Shayne Alamo Busa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ANALYST, 141, 24, 6507, 6509, 2016, [Peer-reviewed], [Corresponding author]
English, Scientific journal

A competitive immunoassay system for microfluidic paper-based analytical detection of small size molecules
Lori Shayne Alamo Busa, Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ANALYST, 141, 24, 6598, 6603, 2016, [Peer-reviewed]
English, Scientific journal

A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed Column
Akihiko Ishida, Mitsutaka Fujii, Takehiro Fujimoto, Shunsuke Sasaki, Ichiro Yanagisawa, Hirofumi Tani, Manabu Tokeshi
ANALYTICAL SCIENCES, 31, 11, 1163, 1169, Nov. 2015, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Fluorescence Polarization Measurement System Using a Liquid Crystal Layer and an Image Sensor
Osamu Wakao, Yusaku Fujii, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi
ANALYTICAL CHEMISTRY, 87, 19, 9647, 9652, Oct. 2015, [Peer-reviewed]
English, Scientific journal

Development of High-performance Immuno-pillar Devices: Improvement of Antibody-immobilized Solid Support
Nanako Nishiwaki, Toshihiro Kasama, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi
BUNSEKI KAGAKU, 64, 5, 329, 335, May 2015, [Peer-reviewed]
Japanese, Scientific journal

An instrument-free, screen-printed paper microfluidic device that enables bio and chemical sensing
Saeed Mohammadi, Masatoshi Maeki, Reza M. Mohamadi, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ANALYST, 140, 19, 6493, 6499, 2015, [Peer-reviewed]
English, Scientific journal

A strategy for synthesis of lipid nanoparticles using microfluidic devices with a mixer structure
Masatoshi Maeki, Tatsuyoshi Saito, Yusuke Sato, Takao Yasui, Noritada Kaji, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Hideyoshi Harashima, Manabu Tokeshi
RSC ADVANCES, 5, 57, 46181, 46185, 2015, [Peer-reviewed]
English, Scientific journal

Bioluminescence Assay Based on Controlled Inhibitory Effect on Firefly Luciferase
Ryoko Kurishiba, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
LUMINESCENCE, 29, 78, 78, Aug. 2014, [Peer-reviewed]
English

Bioluminescence immunoassay using luciferase-encapsulated liposome as a label
Yusuke Nakatani, Chiaki Shido, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
LUMINESCENCE, 29, 83, 84, Aug. 2014, [Peer-reviewed]
English

Effect of reductants on oscillatory chemiluminescence of fluorescein catalyzed by horseradish peroxidase
Hirofumi Tani, Ai Masuyama, Akihiko Ishida, Manabu Tokeshi
LUMINESCENCE, 29, 99, 100, Aug. 2014, [Peer-reviewed]
English

Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes
Tamio Kamidate, Masumi Maruya, Hirofumi Tani, Akihiko Ishida
ANALYTICAL SCIENCES, 25, 9, 1163, 1166, Sep. 2009, [Peer-reviewed]
English, Scientific journal

Microchip reversed-phase liquid chromatography with packed column and electrochemical flow cell using polystyrene/poly(dimethylsiloxane)
Akihiko Ishida, Masamichi Natsume, Tamio Kamidate
JOURNAL OF CHROMATOGRAPHY A, 1213, 2, 209, 217, Dec. 2008, [Peer-reviewed]
English, Scientific journal

Colorimetric method for enzymatic screening assay of ATP using Fe(III)-xylenol orange complex formation
Akihiko Ishida, Yasuko Yamada, Tamio Kamidate
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 392, 5, 987, 994, Nov. 2008, [Peer-reviewed]
English, Scientific journal

Direct determination of horseradish peroxidase encapsulated in liposomes by using luminol chemiluminescence
Tamio Kamidate, Kanako Komatsu, Hirofumi Tant, Akihiko Ishida
ANALYTICAL SCIENCES, 24, 4, 477, 481, Apr. 2008, [Peer-reviewed]
English, Scientific journal

Estimation of the membrane permeability of liposomes via use of eosin Y chemiluminescence catalysed by peroxidase encapsulated in liposomes
Tamio Kamidate, Kanako Komatsu, Hirofumi Tani, Akihiko Ishida
LUMINESCENCE, 22, 3, 236, 240, May 2007, [Peer-reviewed]
English, Scientific journal

Estimation of the contribution of the bioluminescent reaction rate and quantum yield to the enhancement of firefly bioluminescence in the presence of cationic liposomes
Tamio Kamidate, Hirofumi Tani, Akihiko Ishida, Masaya Hayashi
LUMINESCENCE, 22, 1, 15, 19, Jan. 2007, [Peer-reviewed]
English, Scientific journal

Reversed-phase liquid chromatography on a microchip with sample injector and monolithic silica column
Akihiko Ishida, Takahiro Yoshikawa, Masamichi Natsume, Tamio Kamidate
JOURNAL OF CHROMATOGRAPHY A, 1132, 1-2, 90, 98, Nov. 2006, [Peer-reviewed]
English, Scientific journal

Firefly bioluminescent assay of ATP in the presence of ATP extractant by using liposomes
T Kamidate, K Yanashita, H Tani, A Ishida, M Notani
ANALYTICAL CHEMISTRY, 78, 1, 337, 342, Jan. 2006, [Peer-reviewed]
English, Scientific journal

Fluorescein chemiluminescence method for estimation of membrane permeability of liposomes
A Ishida, C Otsuka, H Tani, T Kamidate
ANALYTICAL BIOCHEMISTRY, 342, 2, 338, 340, Jul. 2005, [Peer-reviewed], [Lead author, Corresponding author]
English

Determination of peroxidase encapsulated in liposomes using homogentisic acid gamma-lactone chemiluminescence
T Kamidate, N Kikuchi, A Ishida, H Tani
ANALYTICAL SCIENCES, 21, 6, 701, 704, Jun. 2005, [Peer-reviewed]
English, Scientific journal

Determination of hydrogen peroxide by fluorescein chemiluminescence catalyzed with horseradish peroxidase encapsulated in liposome
T Kamidate, Y Ishida, H Tani, A Ishida
BUNSEKI KAGAKU, 54, 6, 569, 572, Jun. 2005, [Peer-reviewed]
Japanese, Scientific journal

Determination of peroxidase encapsulated in liposomes using homogentisic acid gamma-lactone chemiluminescence
T Kamidate, N Kikuchi, A Ishida, H Tani
ANALYTICAL SCIENCES, 21, 6, 701, 704, Jun. 2005
English, Scientific journal

Determination of hydrogen peroxide by fluorescein chemiluminescence catalyzed with horseradish peroxidase encapsulated in liposome
Tamio Kamidate, Yoshiki Ishida, Hirofumi Tani, Akihiko Ishida
Bunseki Kagaku, 54, 6, 569, 572, Jun. 2005
Japanese, Scientific journal

Cationic liposomes enhanced firefly bioluminescent assay of bacterial ATP in the presence of an ATP extractant
N Nakata, A Ishida, H Tani, T Kamidate
ANALYTICAL SCIENCES, 19, 8, 1183, 1185, Aug. 2003, [Peer-reviewed]
English, Scientific journal

Firefly bioluminescence assay of ATP using diethylaminoethyl-dextran as an enhancer in the presence of sodium chloride and ATP extranctant
A Ishida, T Yoshikawa, T Kamidate
ANALYTICAL BIOCHEMISTRY, 316, 1, 127, 130, May 2003, [Peer-reviewed]
English

Firefly bioluminescence assay of ATP using diethylaminoethyl-dextran as an enhancer in the presence of sodium chloride and ATP extranctant
A Ishida, T Yoshikawa, T Kamidate
ANALYTICAL BIOCHEMISTRY, 316, 1, 127, 130, May 2003
English

Direct detection of horseradish peroxidase as a marker molecule encapsulated in liposomes via use of fluorescein chemiluminescence
T Kamidate, Y Ishida, H Tani, A Ishida
CHEMISTRY LETTERS, 32, 4, 402, 403, Apr. 2003
English, Scientific journal

Preconcentration of catecholamines into liposomes with imposed pH gradients
Akihiko Ishida, Manabu Ikemoto, Yoshiki Ishida, Tamio Kamidate
Bulletin of the Chemical Society of Japan, 76, 5, 985, 989, Chemical Society of Japan, 2003, [Peer-reviewed]
English, Scientific journal

Kamidate, T., Ishida, Y., Tani, H., and Ishida, A.:"Direct Detection of Horseradish Peroxidase as a Marker Molecule Encapsulated in Liposomes via Use of Fluorescein Chemiluminescence", Chemistry Letters,32:402-403 (2003)*　　　　　　　　　　　　　　　
2003

Ishida, A., Ikemoto, M., Ishida, Y., Kamidate, T.: "Preconcentration of Catecholamines into Liposomes with Imposed pH Gradients", Bulletin of the Chemical Society of Japan, 76:985-989 (2003).*　　　　　　　　　　　　　　　
2003

Enhanced firefly bioluminescence assay of ATP in the presence of ATP extractants by using diethylaminoethyl-dextran
A Ishida, T Yoshikawa, T Nakazawa, T Kamidate
ANALYTICAL BIOCHEMISTRY, 305, 2, 236, 241, Jun. 2002, [Peer-reviewed]
English, Scientific journal

Uptake of transition metal ions using liposomes containing dicetylphosphate as a ligand
T Kamidate, Y Hashimoto, H Tani, A Ishida
ANALYTICAL SCIENCES, 18, 3, 273, 276, Mar. 2002, [Peer-reviewed]
English, Scientific journal

Development of simple methods for the visual determination of trace metal ions by using characteristic color-forming reaction systems of metal chelates
Akihiko Ishida
Bunseki Kagaku, 49, 1, 71, 72, Japan Society for Analytical Chemistry, 2000, [Peer-reviewed]
Japanese, Scientific journal

Field screening test for iron leached from well bore rock samples by using color development of iron(III)-xylenol orange system
A Ishida, E Kaneko, T Yotsuyanagi
CHEMISTRY LETTERS, 28, 4, 351, 352, Apr. 1999, [Peer-reviewed]
English, Scientific journal

A New Confined Spot Test with Hydrophobic Filter Paper and Its Application to the Visual Fluorometric Determination of Trace Aluminium with 2,2'-Dihydroxyazobenzene
KANEKO Emiko, ISHIDA Akihiko, DEGUCHI Yuji, YOTSUYANAGI Takao
Chemistry Letters, 1994, 9, 1615, 1618, The Chemical Society of Japan, 05 Sep. 1994
English, A new sensitive and simple spot test has been developed for the visual determination of trace element in water, based on the formation of a size confined colored ring on hydrophobic filter paper. When 0.1 cm³ of aqueous solution is spotted on the water-repelling surface and evaporated in an oven, a small fleck of 6 mm diameter edged with a colored analyte is formed. This method has been successfully applied to the visual fluorometric determination of trace aluminium(III) ion with 2,2′-dihydroxyazobenzene (DHAB or H₂L). The 1 : 1 chelate, [AlL]⁺, becomes localized in a concentric ring zone at pH 6.5. The detection limit is 2 × 10⁻⁸ mol dm⁻³ (0.5 ppb) by visual fluorometry with a UV lamp in the dark.

タンパク質結晶のサイズ分離のためのマイクロフィルターの開発
平山兼光, 真栄城正寿, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2025, 2025

核酸送達のための新規ポリアミノエステル開発
佐藤香織, 真栄城正寿, 渡慶次学, 石田晃彦, 化学系学協会北海道支部冬季研究発表会(Web), 2025, 2025

急性カフェイン中毒診断のための血中レベル迅速測定システム
菊知龍哉, 石田晃彦, 真栄城正寿, 安部寛子, 喜屋武玲子, 上條吉人, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2025, 2025

Synthesis of new discriminative color test reagents for cannabis extracts and elucidation of its mechanism.
新井椋介, 濱島理歩, 西口ひかり, 中村佳代, 安部寛子, 石田晃彦, 渡慶次学, 東恭平, 斎藤顕宜, 高橋秀依, 日本薬学会年会要旨集(Web), 145th, 2025

超小型液体クロマトグラフィーシステムの開発
石田晃彦, フローインジェクション分析講演会講演要旨集, 60th, Nov. 2024, [Invited], [Lead author]

不受胎牛摘発のための乳汁中プロジェステロン濃度測定
柳川洋二郎, 杉浦智親, 石田晃彦, 渡慶次学, 片桐成二, 日本獣医学会学術集会講演要旨集, 167th, 2024

土壌中マグネシウムイオン定量のためのペーパー分析デバイスの開発
永瀬望, 石田晃彦, 日比野光恵, 真栄城正寿, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 73rd, 2024

生物発光紙分析デバイスにおけるルシフェラーゼの活性評価
斉藤潤, 田中楓子, 石田晃彦, 真栄城正寿, 渡慶次学, 田島健次, 谷博文, 日本分析化学会年会講演要旨集(Web), 73rd, 2024

ATPと乳酸測定のための酵素および基質を保持させたペーパーベースの電気化学センサー
菊知龍哉, 石田晃彦, 日比野光恵, 真栄城正寿, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 73rd, 2024

粒径制御型ハイブリッドエクソソーム作製法の開発
大山祥大, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

タンパク質結晶のサイズ分離デバイスの開発とX線結晶構造解析への応用
平山兼光, 真栄城正寿, 真栄城正寿, 真栄城正寿, 上野剛, 坂井直樹, 日比野光恵, 石田晃彦, 山本雅樹, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

深紫外発光ダイオードを光源とするポータブル吸光度検出液体クロマトグラフの開発
小山魁人, 石田晃彦, 日比野光恵, 真栄城正寿, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

タンパク質X線結晶構造解析の自動化のためのオールインワンデバイスの開発
一町田由貴, 真栄城正寿, 真栄城正寿, 真栄城正寿, 上野剛, 小西真昌, 坂井直樹, 日比野光恵, 石田晃彦, 山本雅樹, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

人工エクソソームのRNA送達における膜タンパク質の機能解明
荒谷響子, 丹羽彩由花, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

アプタマーを用いた非競合蛍光偏光分析法における検出感度の向上
千田駿亮, 植咲月, 福山真央, 粕谷素洋, 日比野光恵, 石田晃彦, 真栄城正寿, 火原彰秀, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

マイクロ流体デバイスを用いた核酸搭載ウイルス様粒子作製法の開発
杉浦魁星, 岡田悠斗, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

Development of a SARS-CoV-2-like particles preparation method using microfluidic devices and its application to nucleic acid delivery
杉浦魁星, 岡田悠斗, 真栄城正寿, 真栄城正寿, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム, 23, 1, 2024

マイクロ流体デバイスを用いた人工エクソソームの開発
丹羽彩由花, 大山祥大, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

ポリカチオン/DNA複合体搭載脂質ナノ粒子による効率的な長鎖DNAトランスフェクション
宇野秀哉, 真栄城正寿, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 日比野光恵, 谷博文, 原島秀吉, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

カリウム・硝酸・リン酸イオンの同時定量のための紙分析デバイスの開発
才木陸朗, 石田晃彦, 日比野光恵, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

紙ベースの分析デバイスを用いた生物発光レポーターアッセイ
古坐あすか, 日比野光恵, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

小型測定デバイスからなるATPの簡便かつ迅速な比色定量システム
菊知龍哉, 石田晃彦, 日比野光恵, 真栄城正寿, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 72nd, 2023

LEDベースのUV吸光度検出小型ユニットからなるパームトップ液体クロマトグラフ
小山魁人, 石田晃彦, 日比野光恵, 真栄城正寿, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 72nd, 2023

非競合蛍光偏光分析法の検出感度に影響する因子:トレーサーの蛍光位置および蛍光色素の寿命
千田駿亮, 植咲月, 福山真央, 粕谷素洋, 日比野光恵, 石田晃彦, 真栄城正寿, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

タンパク質のX線結晶構造解析の自動化に向けたマイクロ流体デバイスの開発
一町田由貴, 真栄城正寿, 真栄城正寿, 真栄城正寿, 上野剛, 小西真昌, 坂井直樹, 石田晃彦, 山本雅樹, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

マイクロ流体デバイスを用いたSARS-CoV-2様粒子作製法の開発と薬剤送達への応用
杉浦魁星, 岡田悠人, 真栄城正寿, 真栄城正寿, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

マイクロ流体デバイスを用いたハイブリッドエクソソームの作製
大山祥大, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

Title Development of a portable liquid chromatography system with an electroosmotic pump and chip-based column
石田晃彦, 小山魁人, 日比野光恵, 真栄城正寿, 渡慶次学, キャピラリー電気泳動シンポジウム講演要旨集, 43rd (CD-ROM), 2023

Improved Sensitivity of Bioluminescence Assay in a Paper-Based Analytical Device as a Platform
田中楓子, 大山祥大, 石田晃彦, 真栄城正寿, 渡慶次学, 藤原政司, 田島健次, 谷博文, セルロース学会年次大会講演要旨集, 30th, 2023

神経剤分析用紙製マイクロ流体デバイスの開発
山口晃巨, 宮口一, 石田晃彦, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 71st, 2022

酵素と基質をセルロース媒体に固定化した電気化学ATP-乳酸センサー
九鬼静香, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

ルシフェラーゼ発現大腸菌の紙デバイス上での発現誘導
古坐あすか, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

抗SARS-CoV-2抗体およびSARS-CoV-2スパイクタンパク質断片の非競合蛍光偏光免疫分析法
高橋和希, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

薬剤送達ナノ粒子作製用ガラス製マイクロ流体システムの開発
岡田悠斗, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

タンパク質分析用ペーパー分析デバイスの開発を目的としたろ紙上でのホタルルシフェラーゼ活性の制御
野中康伸, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

タンパク質-リガンド複合体解析のためのマイクロ流体デバイスの開発
舟久保智瑛, 真栄城正寿, 上野剛, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

ルシフェラーゼ発現大腸菌の紙デバイスへの固定化と発現誘導
古坐あすか, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 71st, 2022

エクソソームによるリキッドバイオプシーのための標準脂質ナノ粒子の開発
大山祥大, 丹羽彩由花, 真栄城正寿, 真栄城正寿, 真栄城正寿, 石田晃彦, 谷口正輝, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 71st, 2022

カリウムイオン定量のための距離ベース紙分析デバイスの開発
才木陸朗, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th, 2022

蛍光ナノダイヤモンド搭載セラノスティクス用脂質ナノ粒子の作製
杉浦魁星, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 45th, 2022

マイクロ流体デバイスを用いた人工エクソソーム作製法の開発
丹羽彩由花, 大山祥大, 真栄城正寿, 真栄城正寿, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th, 2022

タンパク質含有ハイドロゲルによるX線結晶構造解析法の開発
一町田由貴, 真栄城正寿, 真栄城正寿, 上野剛, 小西真昌, 坂井直樹, 石田晃彦, 谷博文, 山本雅樹, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 45th, 2022

マイクロ流体デバイスを用いた長鎖プラスミドDNA搭載ナノ粒子の作製
宇野秀哉, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th, 2022

DEVELOPMENT OF AN ELECTROCHEMICAL SENSOR FOR DIAGNOSING SEVERITY USING BLOOD LACTATE / ATP RATIO AS A BIOMARKER
九鬼静香, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, Chemical Sensors, 37, Supplement B, 2021

ポータブル蛍光偏光測定装置を用いたヒト血清中の抗SARS-CoV-2抗体の検出
高橋和希, 西山慶音, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 分析化学討論会講演要旨集(Web), 81st, 2021

Development Of An Electrochemical Sensor For Diagnosing Severity Using Blood Lactate / ATP Ratio As A Biomarker
九鬼静香, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 電気化学秋季大会講演要旨集(CD-ROM), 2021, 2021

マイクロ流体デバイスを用いたプラスミドDNA搭載脂質ナノ粒子の作製
宇野秀哉, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 43rd, 2021

蛍光偏光免疫分析法によるオカダ酸の検出
千田駿亮, 高橋和希, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, ZHERDEV Anatoly V., EREMIN Sergei A., EREMIN Sergei A., 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 44th, 2021

Affibodyを用いた非競合蛍光偏光免疫分析法によるEGFRの検出
高橋和希, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 44th, 2021

蛍光偏光免疫測定法によるヒト血清中の抗SARS-CoV-2抗体の検出
高橋和希, 西山慶音, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, キャピラリー電気泳動シンポジウム講演要旨集, 40th (CD-ROM), 2020

ナノ構造体を用いた脂質ナノ粒子のサイズ分離
清水一樹, 真栄城正寿, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

ペーパーデバイスによる懸濁試料の簡易・迅速分析のための前処理ユニットの開発
前田陵我, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

血中リチウムイオン測定のための全自動分析システムの開発
小松雄士, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

ポータブルHPLCのためのLEDおよびフォトダイオードを用いた小型吸光検出モジュールの開発
西村卓馬, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

ルシフェラーゼの活性制御に基づくオンチップ生物発光アッセイの開発
野中康伸, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

常温X線結晶構造解析のためのマイクロデバイスの開発
舟久保智瑛, 真栄城正寿, 真栄城正寿, 伊藤翔, 伊藤翔, 上野剛, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

生物発光分析のためのペーパーデバイスの開発とキナーゼ分析への応用
高田一生, 渡慶次学, 谷博文, 石田晃彦, 真栄城正寿, 分析化学討論会講演要旨集(Web), 80th, 2020

高感度免疫分析デバイスの開発とH5型鳥インフルエンザウイルス検出への応用
西山慶音, RAMOS Kenia Chavez, 真栄城正寿, 石田晃彦, 谷博文, 笠間敏博, 馬場嘉信, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

乳酸/ATPの血中濃度比による重症度診断のための電気化学酵素センサーの開発
九鬼静香, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

Development of Micro Device for Simple and Rapid Measurement of Progesterone Using Paper Substrates.
小松雄士, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 日本化学会春季年会講演予稿集(CD-ROM), 100th, 2020

紙ベースイムノアッセイのための抗体の簡便な固定化法
前田陵我, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

ナノ構造体搭載デバイスによる脂質ナノ粒子のサイズ分離
清水一樹, 真栄城正寿, 真栄城正寿, 石田晃彦, 谷博文, 西井準治, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

ガラス製マイクロ流体デバイスを用いたsiRNA搭載脂質ナノ粒子の作製と大量生産用集積化デバイスの開発
真栄城正寿, 真栄城正寿, 岡田悠斗, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 日本DDS学会学術集会プログラム予稿集, 36th, 2020

DDSナノ粒子作製用ガラス製マイクロ流体デバイスの開発
岡田悠斗, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

マイクロデバイスを用いたタンパク質結晶の室温複合体構造解析
竹田怜央, 真栄城正寿, 真栄城正寿, 伊藤翔, 伊藤翔, 上野剛, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

UNDERSTANDING THE LIPID NANOPARTICLES STRUCTURE DYNAMICS USING A TIME-RESOLVED SAXS MEASUREMENT
Masatoshi Maeki, Niko Kimura, Kazuki Shimizu, Kento Yonezawa, Nobutaka Shimizu, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi, Proc. Micro TAS 2019, 1478, 1479, Oct. 2019, [Peer-reviewed]
English

PORTABLE FLUORESCENCE POLARIZATION ANALYZER FOR ON-SITE MULTISAMPLE IMMUNOASSAY
Ayano Nakamura, Osamu Wakao, Ken Satou, Mitsutoshi Aoyagi, Kazuhiko Nishimura, Chikaaki Mizokuchi, Ken Sumiyoshi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi, Proc. Micro TAS 2019, 1393, 1394, Oct. 2019, [Peer-reviewed]
English

DEVELOPMENT OF A THREE-DIMENSIONAL MICROMIXER DEVICE FOR PRODUCTION OF VARIOUS LIPID-BASED NUCLEIC ACID NANOCARRIERS
Niko Kimura, Masatoshi Maeki, Yusuke Sato, Kosuke Sasaki, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi, Proc. Micro TAS 2019, 368, 369, Oct. 2019, [Peer-reviewed]
English

HIGH-THROUGHPUT X-RAY CRYSTALLOGRAPHY BASED ON THE PROTEIN CRYASTAL ARRAY
Reo Takeda, Masatoshi Maeki, Sho Ito, Go Ueno, Kunio Hirata, Akihiko Ishida, Hirofumi Tani, Masaki Yamamoto, Manabu Tokeshi, Proc. Micro TAS 2019, 191, 192, Oct. 2019, [Peer-reviewed]
English

FLUORESCENCE SIGNAL AMPLIFICATION FOR SENSITIVE ENZYME IMMUNOASSAY UTILIZING AN IMMUNO-WALL
Keine Nishiyama, Toshihiro Kasama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi, Proc. Micro TAS 2019, 689, 690, Oct. 2019, [Peer-reviewed]
English

フローデバイスを用いたフルオレセイン振動化学発光
池田晋吾, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集, 79th, 2019

マイクロデバイスを用いたタンパク質-リガンド複合体構造解析
真栄城正寿, 真栄城正寿, 竹田怜央, 伊藤翔, 伊藤翔, 上野剛, 平田邦生, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 分析化学討論会講演要旨集, 79th, 2019

電気浸透流ポンプとオンチップカラムに基づく可搬型イオンクロマトグラフの開発
石田晃彦, 西村卓馬, 真栄城正寿, 谷博文, 渡慶次学, イオンクロマトグラフィー討論会講演要旨集, 36th, 2019

核酸搭載脂質ナノ粒子の動的構造解析
真栄城正寿, 真栄城正寿, 木村笑, 清水一樹, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 40th, 2019

蛍光偏光免疫分析法によるH5亜型鳥インフルエンザウイルスの検出
西山慶音, 武田洋平, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 小川晴子, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 40th, 2019

マイクロデバイスを用いたX線結晶構造解析の高速化とリガンドスクリーニングへの応用
竹田怜央, 真栄城正寿, 真栄城正寿, 伊藤翔, 伊藤翔, 上野剛, 平田邦夫, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 39th, 2019

脂質ナノ粒子形成後の二次希釈操作が粒子サイズへ与える影響の解明
木村笑, 真栄城正寿, 岡部奈々, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 39th, 2019

小角X線溶液散乱法による脂質ナノ粒子の動的構造解析法の開発
真栄城正寿, 木村笑, 石田晃彦, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 68th, 2019

電気浸透流ポンプとオンチップカラムに基づくオールインワンコンパクトLCシステムの開発
石田晃彦, 西村卓馬, 真栄城正寿, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 68th, 2019

MICROSCOPIC REAL-TIME MEASUREMENT OF PROTEIN CRYSTAL GROWTH IN MICROFLUIDIC DEVICES
Masatoshi Maeki, Shohei Yamazaki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi, Proc. Micro TAS 2018, 4, 2192, 2193, Nov. 2018, [Peer-reviewed]
English

Microfluidic stepwise and rapid ethanol dilution for precise size control of lipid nanoparticles　　　　　　　　　　　　　　　
Niko Kimura, Masatoshi Maeki, Nana Okabe, Yusuke Sato, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Proc. Micro TAS 2018, 1404, 1405, Nov. 2018, [Peer-reviewed]
English

ナノピラーデバイスを用いたDNAのサイズ分離と無標識検出　　　　　　　　　　　　　　　
阿尻 大雅, 安井 隆雄, 笠 晴也, 真栄城 正寿, 石田 晃彦, 谷 博文, 西井 準治, 馬場 嘉信, 渡慶次 学, 日本分析化学会講演要旨集, 67年会, 125, 125, Aug. 2018
(公社)日本分析化学会, Japanese

電気化学検出液体クロマトグラフィーチップの開発とその尿中シュウ酸分析への応用
藤井大地, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

マイクロ流体デバイスにおけるルミノール化学発光の増感効果
菊地優仁, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

マイクロデバイスを用いた小麦中デオキシニバレノールの多サンプル同時蛍光偏光免疫分析
中村彩乃, 若尾摂, 佐藤憲, 住吉研, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 37th, 2018

微小空間内におけるリゾチームの結晶化制御および晶析挙動解析
山崎翔平, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

Simultaneous Multiplex Determination of Deoxynivalenol in Wheat By Fluorescence Polarization Immunoassay Using Microdevice
中村彩乃, 若尾摂, 佐藤憲, 住吉研, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム, 17, 2, 2018

オンチップ液体クロマトグラフィーのためのウォールジェット式電気化学検出器の開発
干川晃生, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集, 67th, 2018

マイクロデバイスを用いた小型蛍光偏光測定装置の開発とその応用
中村彩乃, 若尾摂, 佐藤憲, 溝口親明, 住吉研, 黒澤文夫, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

ペーパーデバイスによるPOCTのための高感度競合イムノアッセイの実現
前田陵我, 小松雄士, 佐藤優樹, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

ハイスループット蛍光偏光イムノアッセイデバイスの開発
若尾摂, 中村彩乃, 佐藤憲, 溝口親明, 住吉研, 黒澤文夫, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

シクロデキストリンと両親媒性物質を用いたホタルルシフェラーゼの活性制御と生物発光分析法への応用
工藤裕貴, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集, 78th, 2018

酵素免疫測定法を利用した高感度イムノウォールデバイスの開発
西山慶音, 笠間敏博, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

ペーパーデバイスによるリチウムイオン濃度測定
小松雄士, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

微小空間におけるタンパク質結晶成長のリアルタイム計測
竹田怜央, 山崎翔平, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

イムノウォールデバイスの開発と遺伝子変異たんぱく質の検出
中股征哉, 笠間敏博, 笠間敏博, 長谷哲成, 與語直之, 與語直之, 小沢直也, 真栄城正寿, 石田晃彦, 佐藤光夫, 加地範匡, 谷博文, 長谷川好規, 馬場嘉信, 馬場嘉信, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

iLiNPデバイスによるpH応答性カチオン性脂質ナノ粒子の粒径制御
木村笑, 真栄城正寿, 岡部奈々, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 37th, 2018

A Small-Sized Lipid Nanoparticles Production Method Using Microfluidic Devices with Baffle Structures
N. Kimura, M. Maeki, Y. Sato, A. Ishida, H. Tani, H. Harashima, M. Tokeshi, Proc. Micro TAS 2017, 965, 966, Oct. 2017
English

High-Throughput Fluorescence Polarization Measurement System Towards Molecular Interaction Analysis
O. Wakao, M. Maeki, A. Ishida, H. Tani, A. Hibara, M. Tokeshi, Proc. Micro TAS 2017, 557, 558, Oct. 2017, [Peer-reviewed]
English

A Microfluidic-Based Technique for Creating Amyloid Nanostructures and Its Application to Enzyme Reaction
M. Maeki, S. Sato, A. Ishida, H. Tani, M. Tokeshi, Proc. Micro TAS 2017, 1279, 1280, Oct. 2017, [Peer-reviewed]
English

分子間相互作用測定のための多サンプル同時蛍光偏光測定システムの開発
若尾摂, 住吉研, 溝口親明, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, センサ・マイクロマシンと応用システムシンポジウム(CD-ROM), 34th, 2017

小型蛍光偏光イムノアッセイ装置の開発
若尾摂, 住吉研, 溝口親明, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

微小空間によるタンパク質結晶化制御と分光法を用いた晶析挙動解析
山崎翔平, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

マイクロデバイスを用いたアミロイド構造体作製法の開発と酵素反応への応用
真栄城正寿, 佐藤紗耶, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

高効率免疫分析を可能とするイムノウォールデバイスの開発
中股征哉, 笠間敏博, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

DNA分離のためのナノピラーデバイス作製の簡便化とその応用
阿尻大雅, 安井隆雄, 安井隆雄, 笠晴也, 真栄城正寿, 石田晃彦, 谷博文, 西井準治, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, キャピラリー電気泳動シンポジウム講演要旨集, 37th, 2017

バッフル構造を配置した流路デザインによる粒径の精密制御
木村笑, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 35th, 2017

マイクロデバイスを用いた脂質ナノ粒子作製と生体内動態挙動
真栄城正寿, 木村笑, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学工学会秋季大会研究発表講演要旨集(CD-ROM), 49th, 2017

Lipid nanoparticle-size control by microchannel with baffle structures
木村笑, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム, 16, 2, 2017

Development of a New Technique for Washing Steps in Multistep Assays Using Microfluidic Paper-based Analytical Devices
Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi, Proceedings of MicroTAS 2016, 970, 971, Oct. 2016, [Peer-reviewed]
English, Introduction international proceedings

Fluorescence Polarization-based Multiple Samples Detection Using Microchamber Array Towards High-throughput Molecular Interaction Analysis
Osamu Wakao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi, Proceedings of MicroTAS 2016, 1400, 1401, Oct. 2016, [Peer-reviewed]
English, Introduction international proceedings

Effect of the Grooved Structures and the Ethanol Concentration on the Small-sized Lipid Nanoparticles Formation
Yuka Fujishima, Masatoshi Maeki, Yusuke Sato, Takao Yasui, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Hideyoshi Harashima, Manabu Tokeshi, Proceedings of MicroTAS 2016, 1412, 1413, Oct. 2016, [Peer-reviewed]
English, Introduction international proceedings

がん診断のためのエクソソーム分析法の開発　　　　　　　　　　　　　　　
阿尻 大雅, 安井 隆雄, 真栄城 正寿, 石田 晃彦, 谷 博文, 馬場 嘉信, 渡慶次 学, 日本分析化学会講演要旨集, 65年会, 165, 165, Aug. 2016
(公社)日本分析化学会, Japanese

分子間相互作用測定のための蛍光偏光イメージングシステムの開発
若尾摂, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, 分析化学討論会講演要旨集, 76th, 2016

マイクロデバイスを用いたクロラムフェニコールの蛍光偏光免疫測定
青木琴, 若尾摂, 真栄城正寿, 石田晃彦, 谷博文, EREMIN Sergei, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 33rd, 2016

蛍光偏光イメージングによる複数サンプルの同時イムノアッセイ
若尾摂, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

ATP測定のための電気化学酵素センサーの開発
西山慶音, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

マイクロチップ電気化学検出LCデバイスの生体試料分析への応用
藤井大地, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集, 65th, 2016

ルシフェラーゼ内封リポソームを用いたオンチップイムノアッセイ
中谷友祐, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

マイクロデバイスによるタンパク質の立体構造解析
真栄城正寿, 真栄城正寿, 山崎翔平, 杉島正一, 渡邉啓一, 石田晃彦, 谷博文, 宮崎真佐也, 渡慶次学, 分析化学討論会講演要旨集, 76th, 2016

PDMS製チップホルダーを用いたペーパーマイクロ分析チップの開発
MOHAMMADI Saeed, BUSA Lori Shayne Alamo, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 34th, 2016

がん診断のためのエクソソームの無標識検出
阿尻大雅, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 真栄城正寿, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

Label-free detection of extracellular vesicles for cancer diagnosis
Taiga Ajiri, Takao Yasui, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi, Micro Total Analysis Systems 2015, 1789-1791, 1791, 27 Oct. 2015, [Peer-reviewed]
English, Report scientific journal

Fluorescence Polarization Imaging for Multisample Immunoassay
Osamu Wakao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manadu Tokeshi, Proceedings of MicroTAS 2015, 1816, 1818, Oct. 2015, [Peer-reviewed]
English, Introduction international proceedings

Microfluidic Approach for Production of Lipid Nanoparticles-Based Nano Medicine
Masatoshi Maeki, Proceedings of MicroTAS 2015, 838, 840, Oct. 2015, [Peer-reviewed]
English, Introduction international proceedings

脂質ナノ粒子の粒径制御のためのマイクロ流体デバイス設計
真栄城正寿, 藤島由佳, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 32nd, 2015

LCD-CCD Synchronization Detection for Fluorescece Polarization Immunoassay　　　　　　　　　　　　　　　
Osamu Wakao, Yusaku Fujii, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi, Proceedings of the Micro TAS 2014 Symposium, 2271, 2273, Oct. 2014, [Peer-reviewed]
English, Introduction international proceedings

ナノ構造体を用いた無標識DNAの高感度検出
阿尻大雅, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 29th, 2014

マイクロ流路内におけるリポソーム形成メカニズムの考察
齋藤竜亮, 真栄城正寿, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

ナノ構造体の光回折を利用した高感度無標識検出法の開発
阿尻大雅, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

イムノウォールデバイスによる疾病マーカーのマルチプレックスアッセイ
西脇奈菜子, 笠間敏博, 石田晃彦, 谷博文, 馬場嘉信, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

DEVELOPMENT OF 3RD GENERATION IMMUNO-PILLAR DEVICE FOR HIGH SENSITIVE DETECTION OF DISEASE MARKERS
N. Nishiwaki, T. Kasama, A. Ishida, H. Tani, Y. Baba, M. Tokeshi, Micro Total Analysis Systems 2013, 2, 1164-1166, 1166, 28 Oct. 2013, [Peer-reviewed]
English, Report scientific journal

マイクロ流体デバイスを用いるDDSナノキャリアの作製
齋藤竜亮, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 加地範匡, 加地範匡, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 28th, 2013

イムノピラーチップの高性能化
西脇奈菜子, 石田晃彦, 谷博文, 笠間敏博, 笠間敏博, 馬場嘉信, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 27th, 2013

Portable Liquid Chromatography System Based on Battery-Powered Electroosmotic Pump and Microchip with Packed Column and Electrochemical Detector　　　　　　　　　　　　　　　
Akihiko Ishida, Takehiro Fujimoto, Satoshi Yokokawa, Hirofumi Tani, Manabu Tokeshi, Ichiro Yanagisawa, Proceedings of the MicroTAS 2012 Symposium, 1183, 1185, Oct. 2012, [Peer-reviewed]
English, Summary international conference

On-Chip Bioluminescence Assay of ATP and Kinases Using Immobilized Firefly Luciferase in Three-Dimensional Microfluidic Chip　　　　　　　　　　　　　　　
Hirofumi Tani, Atsuki Morisaki, Akihiko Ishida, Manabu Tokeshi, Proceedings of the MicroTAS 2012 Symposium, 1618, 1620, Oct. 2012, [Peer-reviewed]
English, Summary international conference

マイクロチップ液体クロマトグラフィーのためのオンチップ電気化学フローセルの開発
石田 晃彦, 夏目 大道, 谷 博文, Proceedings of the Chemical Sensor Symposium, 42, 97, 99, Sep. 2006
電気化学会化学センサ研究会, Japanese

日本分析化学会北海道支部編「水の分析(第5版)」<共著>　　　　　　　　　　　　　　　
化学同人, 2005

「リポソーム応用の新展開」～人工細胞の開発に向けて～<共著>　　　　　　　　　　　　　　　
エヌ・ティー・エス, 2005

Highly sensitive visual fluorometry of aluminium at ppb level with ring-like solid phase of poly(vinyl alcohol)
A Ishida, E Kaneko, T Yotsuyanagi, CHEMISTRY LETTERS, 3, 217, 218, Mar. 1999
English

マイクロ流体分析　　　　　　　　　　　　　　　
渡慶次 学, 真栄城 正寿, 佐藤 記一, 佐藤 香枝, 火原 彰秀, 石田 晃彦, Chapter5 マイクロチップクロマトグラフィー
Oct. 2020, [Joint work]

農産物・食品検査法の新展開 《普及版》
山本, 重夫
シーエムシー出版, Aug. 2016, 9784781311166, x, 265p, Japanese

農産物・食品検査法の新展開 = Advanced inspection technology of agricultural products and foods
山本, 重夫, 第IV編第1章色で見分ける細菌汚染スクリーニング法～新しいキシレノールオレンジ-鉄錯体法の技術～
シーエムシー出版, Jul. 2010, 9784781302478, x, 265p, Japanese, [Contributor]

水の分析
日本分析化学会北海道支部, 厚谷, 郁夫, 田中, 俊逸, 4.7 フローインジェクション分析（FIA）
化学同人, Jun. 2005, 9784759809916, x, 472p, Japanese, [Contributor]

リポソーム応用の新展開 : 人工細胞の開発に向けて
秋吉, 一成, 辻井, 薫, 奥, 直人, 久保井, 亮一, 宝谷, 紘一, 第4節リポソームを利用した高感度計測法
エヌ・ティー・エス, Jun. 2005, 4860430859, 4, 10, 683, 10, 12p, 図版14p, Japanese, [Contributor]

北海道地域3大学新技術説明会にて成果発表　　　　　　　　　　　　　　　
2012

第２４回国際計量計測展にて研究成果の発表と試作品の展示　　　　　　　　　　　　　　　
2010

第3回次世代医療システム産業化フォーラム2010にて技術発表　　　　　　　　　　　　　　　
2010

北海道大学・科学技術振興機構主催の北海道大学 新技術説明会にて技術発表　　　　　　　　　　　　　　　
2009

北海道大学工学系イノベーションブリッジ2007にて研究成果発表　　　　　　　　　　　　　　　
2007

農業の生産性と持続性の向上を支援する簡便・低コストな画期的スマート土壌診断システムの基盤技術の開発　　　　　　　　　　　　　　　
オープンイノベーション研究・実用化推進事業基礎研究ステージ（基礎重要政策タイプ）
Jul. 2023 - Mar. 2026
渡慶次学, 石田晃彦, 丸山隼人, 細淵幸雄, 松田憲司
生物系特定産業技術研究支援センター, 北海道大学・北海道立総合研究機構・BIPROGY株式会社, Coinvestigator, 05010a3

大麻成分検出ペーパーデバイス開発
産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 産学共同(本格型)
2022 - Mar. 2026
安部寛子, 渡慶次 学, 高橋秀依
近年、違法な大麻栽培や大麻乾燥物の国内流通が増加しており、捜査機関はその取り締まりに苦慮している。また、大麻草に含有されるカンナビノイド類にリラクゼーション・鎮痛作用等が謳われ、大麻草抽出物がセルフメディケーション製品として市販され、健康被害も報告されている。そのため、捜査機関や化粧品、食品業界からは、法規制成分であるカンナビノイド(THC)を精度よく簡便に検出する技術に高い関心が集まっている。そこで本事業では、このような製品中にTHCが含有されているか否かを簡単に正確に判定するペーパーデバイスを開発する。公的機関で利用される信頼性のあるデバイスの開発を行い、安心・安全な社会づくりに貢献する。
科学技術振興機構, 株式会社バイオデザイン, Coinvestigator not use grants, JPMJTR224B

乳牛の分娩間隔短縮技術改良・実証事業　　　　　　　　　　　　　　　
2024 年度日本中央競馬会畜産振興事業
2024 - 2026
公益財団法人 全国競馬・畜産振興会, 北海道大学, Coinvestigator

育種・染色体操作を用いたサケ科魚類の魚卵アレルゲン性低減化の試み
科学研究費助成事業 基盤研究(C)
01 Apr. 2020 - 31 Mar. 2023
清水 裕, 笹岡 友季穂, 藤本 貴史, 平松 尚志, 石田 晃彦, 渡慶次 学, 佐伯 宏樹
進捗状況を3項目に分けて述べる。
1.不妊魚の作出：ニジマスの卵と凍結保存したブラウントラウトあるいはサクラマスの精子を受精し、第二極体放出阻止処理により作出された雑種三倍体候補（A：ニジマス×ブラウントラウト、B：ニジマス×サクラマス）の倍数性を調査した。その結果、候補Aでは全25個体で、候補Bでは1個体を除く30個体が三倍体であった。これらの個体はPITタグで標識し、現在も継続して飼育している。（藤本）
2.魚卵アレルゲン検知系の構築：引き続き、ニジマス卵アレルゲンであるβ’-component（BC）のペーパー免疫分析デバイスの構築に取り組んだ。デバイスは濾紙にインクを印刷して加熱する常法により作製し、インクで囲まれた領域を分析反応ゾーンとした。分析は、反応ゾーンに新規作製した抗BC抗体を固定化して試料、酵素標識抗BC抗体、発色試薬を順に加えて行う手順とした。本手法では、従来並みの感度（検出感度：約1 ng/mL）の分析が従来の1/100の時間（約20分）で可能となった。（渡慶次、石田）
加えて、交雑種に対応した検知系に使用する抗体の作成のため、サクラマス排卵からBCを精製し、これを家兎に免疫し抗血清を作製した。（平松）
3.不妊化魚の魚卵アレルゲン性の調査：全23個体の不妊化三倍体ニジマスの内臓組織に含まれるBCを測定したが、全個体の筋肉には魚卵アレルギー発症リスクが認められなかった。しかし、5個体において生殖線から少量のBCが検出されたが、その内3個体は目視にて明確な生殖腺の発達が観られた。目視で生殖線の発達が確認できなかった20個体について、生殖腺の組織切片を作製・観察し、その発達状況を確認した。その結果、３個体で発達中の卵母細胞が散在しているのが確認され、生殖腺の外観だけでアレルギー発症リスクの有無を見分けるのは困難であることが判明した。（清水、佐伯、笹岡、平松）
日本学術振興会, 基盤研究(C), 北海道大学, 20K05903

Construction of a hybrid electrochemical micro-cell and its application to the analyses of trace biological substances
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2017 - 31 Mar. 2020
Ishida Akihiko
An electrochemical detector for next-generation miniaturized analytical devices for testing blood and urine has been developed. Miniaturization of devices allowing automatic flow-based measurement is one of the worldwide topics because it enables rapid diagnosis near the patient. These devices also require improving sensitivity. However, increasing the electrode area is counterproductive, and no other approach has been attempted. Therefore, this study focused on not the electrode area, but the geometry of the electrode. It demonstrated that the sensitivity was successfully improved by changing the geometry of the conventional shape of a disc to a concentric array of multiple ring electrodes. The proposed array electrode allowed the detection of a trace amount of dopamine in blood without special pre-concentration of a sample.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 17K05892

2018年度 現場での即時検査が可能な高性能可搬型分析装置の開発 補助事業　　　　　　　　　　　　　　　
研究補助開発研究
Apr. 2018 - Mar. 2019
石田 晃彦
JKA, Principal investigator, Competitive research funding

ポータブル分析装置の高感度なマイクロ検出器の開発　　　　　　　　　　　　　　　
研究成果展開事業（研究成果最適展開支援プログラム（A-STEP））フィージビリティスタディ（ＦＳ）ステージ 探索タイプ
Jan. 2015 - Dec. 2015
石田 晃彦
科学技術振興機構, Principal investigator, Competitive research funding

Development of next-generation diagnostic chip and its application to clinical diagnosis
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
31 May 2012 - 31 Mar. 2015
TOKESHI Manabu, YUZAWA Yukio, AKIYAMA Shinichi, TANI Hirofumi, ISHIDA Akihiko
We developed a diagnostic chip with a five biomarker panel for diabetic nephropathy. The biomarker panel of MCP-1, L-FABP, Angiotensinogen, CTGF, and Collagen IV was adopted for diabetic nephropathy. With standard and patient samples, we evaluated the performance of the panel diagnostic chip. For the detection of biomarkers, we confirmed the chip provides rapid analysis (total assay time of 12 min) with high sensitivity and it uses small volumes of the sample and reagent (0.5 μL each), and the obtained results correlated with that of conventional ELISA.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 24245013

Construction of a highly sensitive electrochemical detector on a microchip device with an integrated single comb-like array electrode and its application
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2011 - 2013
ISHIDA Akihiko
In this study, a single comb-like electrode was studied, which was formed by arranging a number of microelectrodes at a given spacing. A thorough examination of the shape factors of the electrode led to electrochemical detection of more trace amounts of substances. High sensitivity has been achieved by the electrode alone with a characteristic shape. The electrode has good utility because a wide variety of target substances can be detected and the detection can be performed with a relatively cheap measuring apparatus. This electrode is expected to be used as a powerful detector for microfluidic analysis devices possibly demanded in a field of clinical diagnosis in near future.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 23550087

刺激応答性ベシクルを利用する高感度電気化学検出バイオ分析チップの開発
科学研究費助成事業 若手研究(B)
2006 - 2007
石田 晃彦
本研究では,微小流路内においてベシクル内から漏出させた電気化学活性物質をアンペロメトリーにより高感度に検出することが重要な課題である。電流値は電極面内に形成する測定物質の濃度勾配の積算値であるから,感度を上げるには作用電極の面積または濃度勾配を大きくすることが必要である。これまでに,電極面積を大きくすることは,S/N比を低下させるため有効ではないことを微小流体デバイスを用いて示した。これは,物質が電極反応により消費されながら流れる結果,電極の下流ほど電極垂直方向の濃度勾配が小さくなるためである。そこで,与えられた面積の中で濃度勾配を増加させることが必要になる。そのため,本研究では一本の電極を複数に分割して間隔を置いて配置するくし状の電極を検討した。電極がない部分で試料が補充されるので,下流側での濃度勾配の減少を抑えることができるからである。さらに十分な間隔があれば,分割したどの電極上でも同様の濃度勾配が形成されると推察される。くし形の電極については,これまでに2対の電極を用いて酸化還元を繰り返して感度を上げる検討は行われていたが,くし形電極単独での詳細な検討はなかった。そこで,ポリマー基板およびポリジメチルシロキサンを用いて,間隔をあけた2本の作用電極をもつ微小流体デバイスを作製し,このアプローチの妥当性を検証した。その際,微小流体デバイスで用いられる流速範囲で,物質の種類を変えることにより電流応答の拡散係数依存性を調べた。その結果,どの拡散係数およびどの流速でも間隔の増加とともに電流値は増加し,200μm近辺で最大一定となった。また,電流値を様々な拡散係数および流速で数値シミュレーションしたところ実験結果と一致した。以上から,このアプローチが妥当であり,微小流体デバイスで用いられる流速で様々な電気活性物質に適用できることを明らかにした。
日本学術振興会, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 18750056

The development of nanor eactor for high sensitive chemi luminescence analysis by using liposomes
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2006 - 2007
KAMIDATE Tamio, TANI Hirofumi, ISHIDA Akihiko
Horseradish peroxidase (HRP) -encapsulated liposomes were prepared by freeze-thawing method. The number of HRP molecules trapped in liposomes was three times greater than that prepared by extrusion method HRP trapped in liposomes was directly detected by using luminol chemiluminescence (CL) with H_2O_2 without lysis of liposomes. The CL measurement conditions in both a lipid-free bulk solution and in liposomes were optimized in the concentrations of luminol and H_2O_2 by measuring the CL response curves, in which only one peak appeared and the CL intensity was maximal. The CL intensity observed in HRP-catalysed luminol CL in liposomes was a factor of seven greater than that observed in a lipid-free bulk solution. The detection limit in the direct detection of HRP encapsulated in liposomes was sensitive by a factor of 3 compared with that in HRP-catalysed luminol CL in a lipid-free bulk solution. In addition, HRP-trapped liposomes were combined to antibody in order to apply HRP-trapped liposomes to the marker in immunoassay. The CL intensity observed in antibody combined HRP-trapped liposomes was greater 125 times that in antibody combined HRP by avidin-biotin bond.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 18350037

化学発光速度を指標とするリポソームの膜透過性の評価法の開発
科学研究費助成事業 萌芽研究
2005 - 2006
上舘 民夫, 谷 博文, 石田 晃彦
1.エオシンYを用いる膜透過性の評価
リボソームの内水相にペルオキシダーゼ(POD)を封入し、その外水相にエオシンYと過酸化水素を添加すると、エオシンYと過酸化水素は迅速に膜を透過し、内水相においてPODを触媒とする化学発光反応が進行した。そこで、リン脂質であるフォスファチジルコリンに対して30〜45%のコレステロール含量を有するリボソームにPODを封入し、発光応答曲線を測定した。その結果、コレステロール含量が増大するほど発光初期速度は減少した。この結果から、エオシンYの発光初期速度が膜透過性を反映することがわかった。
2.ピレン法による膜流動性の評価
コレステロール含量が増大するほど発光初期速度は遅くなる原因として、コレステロール含量が増大するほどリボソームの膜流動性が減少することが考えられる。そこで、30〜45%のコレステロール含量を有するリボソームに蛍光プローブであるピレンを加え、膜流動性を評価した。その結果、コレステロール含量が増大するほど、膜流動性が減少した。したがって、エオシンYの発光初期速度を用いる方法が膜透過性の評価法として利用できることが明らかになった。
3.膜透過性の速度論的解析
リボソーム内へのエオシンYと過酸化水素の透過と化学発光反応を考慮して、発光初期速度を表す速度式を解析した。その結果、発光初期速度は膜透過速度定数、反応速度定数および基質初濃度で表された。また、反応速度定数および基質初濃度が一定のとき、発光初期速度は膜透過速度定数に比例することがわかった。エオシンYの膜透過速度定数を求めたところ、3.02x10^<-3> s^<-1>の値になった。
日本学術振興会, 萌芽研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 17655028

液体クロマトグラフィーの高性能マイクロシステムの構築とその生体分子分析への応用
科学研究費助成事業 若手研究(B)
2004 - 2005
石田 晃彦
本課題の目的は,液体クロマトグラフィー(LC)の要素をガラスやポリマーチップ上に集積化したLCチップを作製し,これを生体分子の分離分析に適用してその有用性を実証することである。本年度は検出部である電極の面積が分離性能および感度(S/N比)に与える影響を検討した。その際,LCチップ間で生じる分離カラムの性能誤差が応答に影響しないよう同一の分離カラムで検討するために,分離カラムから出たバンドを分岐させ,それぞれ異なる電極で同時に検出するLCチップを作製した。電極は金をチップ上に蒸着することにより作製した。分岐部でバンドが等分されることについては蛍光顕微鏡法により確認した。このLCチップを用いて検討した結果,分離度は電極面積に依存しないことを確認した。これは分離カラム(2.5cm長,約10000段/m)から出た試料のバンド幅が電極幅よりも十分に大きいためである。バンド幅と理論段の関係を考慮すると,この結果は小さな分離カラムをもつLCチップ全般にあてはまるといえる。一方,S/N比に関しては電極面積が大きいほど減少した。この理由を電極表面上の試料の流体・拡散シミュレーションに基づいて考察した。すなわち,試料の電極反応が電極上流部で終了するため,電極面積が大きいほど下流部で面積過剰となり電極単位面積当たりに得られる電流値が減少するためと考察した。電極幅は50μm幅が最も有効であった。最後に,医学的な効果が報告されているカテキン類((+)-カテキン,エピカテキン,没食子酸エピガロカテキン)の分離を試みた。その結果,ベースライン分離を達成し,それぞれの検出限界は数百nMレベルであった。また,市販の各種茶飲料のカテキン分析に応用したところ,各成分の分離に加えて,茶葉の発酵の程度が各試料のクロマトグラムに反映していることが確認でき,実試料に対しても良好に適用できることを明らかにした。
日本学術振興会, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 16750056

The investigation for high sensitive chemi luminescence method using nanoreactor as a marker
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2003 - 2004
KAMIDATE Tamio, TANI Hirofumi, ISHIDA Akihiko
Liposomes encapsulated horseradish peroxidase(HRP) were prepared for applying the inner water phase in liposome to the nanoreactor for HRP-catalyzed chemiluminecence(CL) reaction. Phosphatidylcholine(PC), phosphatidylglycerol dimyristoyl(DMPG) and cholesterol were used as a component for liposome. The mole % of PC : DMPG : Chel was 8:1:1. The HRP-trapped liposome was prepared by extrusion method with polycarbonate filter. Homogentisic acid γ-lactone(HAL) and luminol were used as a CL reagent for the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in ρ-iodophenol (ρ-IP) enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in ρ-IP enhanced CL methods, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in ρ-IP enhanced lnminol CL. The linear range of calibration curve for HRP in HAL CL was improved by factors of 50 compared with that in ρ-IP enhanced luminol CL. From these results, it was found that HAL CL were superior to ρ-IP enhanced luminol CL for the determination of HRP encapsulated in liposomes.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, Coinvestigator not use grants, Competitive research funding, 15350038

高輝度光微細加工による生体内物質分析用微小電気化学反応セル創製に関する研究
科学研究費助成事業 萌芽研究
2003 - 2004
坂入 正敏, 石田 晃彦
本研究課題は、高輝度光(集光したパルスYAGレーザー照射)表面微細加工法と電気化学的手法により、酵素反応によって生体内で生成される過酸化水素などの、生体微量物質分析用微小電気化学セルをアルミニウム上に作製すること、さらに、対極などを取り付け微小電気化学分析システムにして、その性能を評価することを目的としている。
昨年度は、アルミニウムを用いてアノード酸化、レーザー加工、電気めっきを用いて、2電極型システムを作製でき、K_3Fe(CN)_6/K_4Fe(CN)_6の混合溶液(約1.5μl)を用いてCV測定を行ったところ、アノードピーク電流の走査速度依存性や濃度依存性を確認できたが、ピーク形状および液漏れの問題があった。そこで、本年度は、流路などの形状の改良および作製工程の改良を行い、より完成度の高い生体微量物質分析用微小電気化学セルの作製およびその電気化学的特性評価を行った。
微細溝および微細貫通孔には、レーザー照射後、再度アノード酸化することで、保護性の皮膜を形成し、電極部にはめっきにより金を析出させた。これら、再アノード酸化および金めっきにより、各種溶液で安定に動作可能な、理論内容量1.5μlの微小電気化学セルを作製できた。作製したセルを用いて、電気化学測定を行った結果、従来のマクロ電極と同様のサイクリックボルタモグラムが測定できた。すなわち、アノードピーク電流は電位走査速度の平方根に比例して増加し、その傾きから電極面積を見積もった結果、妥当な値を得ることができた。
日本学術振興会, 萌芽研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 15656177

自己組織化システムを用いる高性能サイズ制御スポットテスト法
科学研究費助成事業 特別研究員奨励費
1998 - 1998
石田 晃彦
日本学術振興会, 特別研究員奨励費, 東北大学, 97J06075

マイクロチップを利用する化学分析システムの開発　　　　　　　　　　　　　　　
Competitive research funding

血液検体のＡＴＰ測定方法及びキット　　　　　　　　　　　　　　　
Patent right, 石田 晃彦
特許6295408
02 Mar. 2018

ポータブルな液体クロマトグラフ及び液体クロマトグラフィー　　　　　　　　　　　　　　　
Patent right, 石田 晃彦
特許5935696
20 May 2016

検体中に含まれるアデノシン三リン酸の分析方法
Patent right, 石田 晃彦, 山田 泰子, 上舘 民夫, 国立大学法人 北海道大学
JP2006308601, 25 Apr. 2006
WO2006-118093, 09 Nov. 2006
特許第4940432号
30 May 2012
200903081314391360

細胞中ATPの定量法
Patent right, 上舘 民夫, 谷 博文, 石田 晃彦, 三菱化学メディエンス株式会社
特願2004-061585, 05 Mar. 2004
特開2005-245342, 15 Sep. 2005
特許第4546115号
09 Jul. 2010
201103016902185272

イオン性検体のスポットテスト法
Patent right, 四ツ柳 隆夫, 金子 恵美子, 宮川 貴行, 石田 晃彦, 株式会社東北テクノアーチ
特願平6-048632, 18 Mar. 1994
特開平7-260693, 13 Oct. 1995
特許第3393701号
31 Jan. 2003
201103034197155383

試料中成分溶出量の簡易判定方法及びその簡易判定用キット
Patent right, 石田 晃彦, 金子 恵美子, 四ツ柳 隆夫, 株式会社東北テクノアーチ
特願平10-177968, 21 May 1998
特開平11-326309, 26 Nov. 1999
200903018923337891