鈴木 裕 (スズキ ユタカ)
| 北方生物圏フィールド科学センター 耕地圏ステーション 生物生産研究農場 | 准教授 |
Last Updated :2025/03/17
■研究者基本情報
プロフィール情報
乳牛や肉牛の胃袋は、人類が利用できない牧草などの植物性繊維をエネルギーや蛋白質に変換する素晴らしい機能を持っています。しかし、生まれたばかりの子牛ではほどんど働いていません。ウシの消化機能の鍵となる共生微生物や、宿主の消化管組織（反芻胃：ルーメン）が発達する過程を細胞・分子レベルで明らかにし、ウシが持つ資源変換能力を高めることを目指した研究を行っています。また、子牛は免疫機能が弱く、下痢などの疾患に罹りやすい状態にあります。腸管免疫に関する研究も行っており、ウシの健康な生育をサポートすることで生産ロスや環境負荷を低減し、持続可能な生産システムへの貢献を目指しています。
研究室ウェブサイト：https://sites.google.com/view/huenvsci-suzukilab/home
Researchmap個人ページ
J-Global ID
■経歴
委員歴
■研究活動情報
受賞
- 2020年03月, 日本畜産学会, Animal Science Journal Excellent Paper Award
Utilization of digital differential display to identify differentially expressed genes related to rumen development
Kato, D.;Suzuki, Y.;Haga, S.;So, K.;Yamauchi, E.;Nakano, M.;Ishizaki, H.;Choi, K.;Katoh, K.;Roh, S. - 2018年03月, 日本畜産学会, 奨励賞（第111号）
鈴木裕 - 2016年03月, 東北大学大学院農学研究科, 研究科長賞
鈴木裕 - 2016年03月, 日本畜産学会, Animal Science Journal Excellent Paper Award
Chemerin analog regulates energy metabolism in sheep
Suzuki, Y.;Song, S.;Sato, K.;So, K.;Ardiyanti, A.;Kitayama, S.;Hong, Y.;Lee, S.;Choi, K.;Hagino, A.;Kazuo, K.;Roh, S.
論文
- TLR5 ligand induces the gene expression of antimicrobial peptides and CXCL8 through IL-1β gene expression in cultured rumen epithelial cells.
Koki Nishihara, Yutaka Suzuki, Satoshi Haga, Sanggun Roh
Animal science journal = Nihon chikusan Gakkaiho, 95, 1, e13972, 2024年, ［国際誌］
英語, 研究論文（学術雑誌）, High grain feeding or weaning, which could compromise the rumen epithelium by increasing ruminal short-chain fatty acid (SCFA) concentrations with pH reduction, is associated with high levels of ruminal toll-like receptor 5 (TLR5). This study aimed to determine the role of TLR5 in the rumen epithelium. Immunohistochemistry revealed that TLR5 was localized in cells on the basal side (i.e., basal and spinous layers) rather than in the granular layer in the rumen epithelium, where tight junctions are most potent, in pre- and post-weaning calves (n = 9). Primary bovine rumen epithelial cells (BRECs) obtained from Holstein cows (n = 3) were cultured to investigate the factors that upregulate TLR5; however, SCFA, low pH (pH 5.6), BHBA, L-lactate, D-lactate, and LPS did not upregulate TLR5 gene expression in BREC. Primary BREC treated with flagellin (TLR5 ligand) had higher expression of interleukin-1β (IL-1β) (P < 0.05) than BREC treated with vehicle. In addition, BREC treated with IL-1β had higher expression of antimicrobial peptides and C-X-C motif chemokine ligand 8 than BREC treated with vehicle (P < 0.05). These results suggest that ruminal TLR5 may recognize epithelial disruption via flagellin and mediate the immune response via IL-1β during high-grain feeding or weaning. - In vitro Effects of Cellulose Acetate on Fermentation Profiles, the Microbiome, and Gamma-aminobutyric Acid Production in Human Stool Cultures
Hiroaki Yamada, Hiroto Miura, Yutaka Suzuki, Satoshi Koike, Shu Shimamoto, Yasuo Kobayashi
Current Microbiology, 80, 9, 284, 284, Springer Science and Business Media LLC, 2023年07月14日, ［国際誌］
英語, 研究論文（学術雑誌）, Gamma-aminobutyric acid (GABA) is considered as a potential candidate substance that mediates the effects of intestinal bacteria on human mental health. In the present study, we evaluated the effect of water-soluble cellulose acetate (WSCA), a type of cellulose ester, on fermentation and microbial profiles, and GABA production in human stool cultures prepared from fresh feces from volunteers. In addition, the GABA-producing ability of Bacteroides uniformis, which can utilize WSCA, was evaluated in a pure-culture study. All incubations were conducted anaerobically. WSCA supplementation increased (P < 0.05) acetate and propionate production and decreased (P < 0.05) the pH in human fecal cultures. WSCA significantly altered the microbiota, selectively increasing the relative abundance of B. uniformis (P < 0.05). Pure-culture study results revealed that B. uniformis produces GABA, possibly via a glutamate-dependent acid resistance system under low pH conditions. In conclusion, WSCA could be a potential prebiotic material that is fermented by intestinal bacteria and increases short-chain fatty acid and GABA production in the human gut. Bacteroides uniformis might play an important role in both WSCA degradation and GABA production in the intestine. - Batch culture analysis to identify potent organic acids for suppressing ruminal methane production.
Kyouko Yamada, Keiichiro Iwamae, Yutaka Suzuki, Satoshi Koike, Yasuo Kobayashi
Animal science journal = Nihon chikusan Gakkaiho, 94, 1, e13873, 2023年, ［国際誌］
英語, 研究論文（学術雑誌）, We performed an in vitro rumen batch culture study to screen 11 commercially available organic acids for methane-suppressing ability and analyzed the rumen microbiota to determine the mode of action of the acids that showed potent methane-suppressing activity. Nine of the 11 acids showed methane-suppressing activity. Maleic anhydride, itaconate, citrate, and fumarate, which showed the highest activity, were further examined. These four acids showed methane-suppressing activity irrespective of the hay-to-concentrate ratios of the substrate. Maleic anhydride and itaconate decreased total gas and short-chain fatty acid production. Maleic anhydride and fumarate increased propionate production, while itaconate increased butyrate production. Maleic anhydride, itaconate, and citrate increased lactate production. Fumarate increased the abundance of bacteria involved in propionate production. Maleic anhydride, itaconate, and citrate increased the abundance of bacteria involved in lactate production. Thus, the results indicate that maleic anhydride, itaconate, and citrate may decrease methane in part by stimulating the acrylate pathway. - Physiological roles and regulation of hepatic angiopoietin-like protein 3 in Japanese Black cattle (Bos taurus) during the fattening period
Rika Shikida, Minji Kim, Makoto Futohashi, Koki Nishihara, Huseong Lee, Yutaka Suzuki, Yeolchang Baek, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Yoshinobu Uemoto, Satoshi Haga, Fuminori Terada, Sanggun Roh
Journal of Animal Science, 101, Oxford University Press (OUP), 2023年01月01日, ［国際誌］
英語, 研究論文（学術雑誌）, Abstract
Angiopoietin-like protein 3 (ANGPTL3) is expressed predominantly in the liver and plays a major role in regulating the circulating triglyceride and lipoprotein fraction concentrations by inhibiting lipoprotein lipase (LPL) activity. Given these physiological roles, ANGPTL3 may play an important role in metabolic changes related to fat accumulation during the fattening period in Japanese Black. This study aimed to reveal the physiological roles of hepatic ANGPTL3 in Japanese Black steers (Bos taurus) during the fattening period and investigate the regulatory effects of hepatic ANGPTL3. To investigate the gene expression and protein localization of ANGPTL3, 18 tissue samples were collected from tree male Holstein bull calves aged 7 wk. Biopsied liver tissues and blood samples were collected from 21 Japanese Black steers during the early (T1; 13 mo of age), middle (T2; 20 mo), and late fattening phases (T3; 28 mo). Relative mRNA expression, blood metabolite concentrations, hormone concentrations, growth, and carcass traits were analyzed. To identify the regulatory factors of hepatic ANGPTL3, primary bovine hepatocytes collected by two Holstein calves aged 7 wk were incubated with insulin, palmitate, oleate, propionate, acetate, or beta-hydroxybutyric acid (BHBA). The ANGPTL3 gene was most highly expressed in the liver, with minor expression in the renal cortex, lungs, reticulum, and jejunum in Holstein bull calves. In Japanese Black steers, relative ANGPTL3 mRNA expressions were less as fattening progressed, and blood triglyceride, total cholesterol, and nonesterified fatty acid (NEFA) concentrations increased. Relative ANGPTL8 and Liver X receptor alpha (LXRα) mRNA expressions decreased in late and middle fattening phases, respectively. Furthermore, relative ANGTPL3 mRNA expression was positively correlated with ANGPTL8 (r = 0.650; P < 0.01) and ANGPTL4 (r = 0.540; P < 0.05) in T3 and T1, respectively, and LXRα showed no correlation with ANGPTL3. Relative ANGTPL3 mRNA expression was negatively correlated with total cholesterol (r = −0.434; P < 0.05) and triglyceride (r = −0.645; P < 0.01) concentrations in T3 and T1, respectively; There was no significant correlation between ANGTPL3 and carcass traits. Relative ANGTPL3 mRNA expression in cultured bovine hepatocytes was downregulated in oleate treatment. Together, these findings suggest that ANGPTL3 downregulation in late fattening phases is associated with the changes in lipid metabolism. - Changes in immunoglobulin A and immunoglobulin G concentrations in the saliva of Japanese Black beef cows during calving: a pilot study
Satoshi Haga, Yutaka Suzuki, Masafumi Miwa, Kaiyu Kubota, Miki Sakatani
Animal Science Journal, 93, 1, e13793, Wiley, 2022年12月10日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Immunoglobulin A (IgA) in saliva, mostly consisting of secretory IgA, plays an important role in the mucosal immune mechanism. This study evaluated changes in salivary IgA and Immunoglobulin G (IgG) concentrations in Japanese Black cows (n = 16) during calving. Individual saliva samples were collected -2, 0, and 2 weeks postpartum. Immunoglobulin concentrations differed significantly among weeks (P < 0.05), but the effect of parity and week × parity was insignificant. Salivary IgA concentrations decreased drastically (P < 0.05) after calving compared with those at -2 weeks postpartum and remained low until 2 weeks postpartum. The salivary IgG concentrations decreased gradually during peripartum and differed at -2 and 2 weeks postpartum (P < 0.05). Considering the immunoglobulin concentrations at -2 weeks postpartum as the reference standard for 100%, the rates of decrease in IgA concentrations (36.7 ± 6.9%) were significantly lower (P < 0.05) than those of IgG (70.3 ± 10.1%) at calving day. To our knowledge, this is the first report indicating that salivary IgA concentrations decreased drastically after calving in Japanese Black cows. Further studies monitoring the secretory functions of IgA in the salivary gland are essential for understanding maternal immunity in cattle. - Alterations in rumen microbiota via oral fiber administration during early life in dairy cows
Heshan Kodithuwakku, Daiki Maruyama, Hisashi Owada, Yuto Watabe, Hiroto Miura, Yutaka Suzuki, Kazuo Hirano, Yasuo Kobayashi, Satoshi Koike
Scientific Reports, 12, 1, 10798, 10798, Springer Science and Business Media LLC, 2022年12月, ［国際誌］
英語, 研究論文（学術雑誌）, Abstract
Bacterial colonization in the rumen of pre-weaned ruminants is important for their growth and post-weaning productivity. This study evaluated the effects of oral fiber administration during the pre-weaning period on the development of rumen microbiota from pre-weaning to the first lactation period. Twenty female calves were assigned to control and treatment groups (n = 10 each). Animals in both groups were reared using a standard feeding program throughout the experiment, except for oral fiber administration (50–100 g/day/animal) from 3 days of age until weaning for the treatment group. Rumen content was collected during the pre-weaning period, growing period, and after parturition. Amplicon sequencing of the 16S rRNA gene revealed that oral fiber administration facilitated the early establishment of mature rumen microbiota, including a relatively higher abundance of Prevotella, Shuttleworthia, Mitsuokella, and Selenomonas. The difference in the rumen microbial composition between the dietary groups was observed even 21 days after parturition, with a significantly higher average milk yield in the first 30 days of lactation. Therefore, oral fiber administration to calves during the pre-weaning period altered rumen microbiota, and its effect might be long-lasting until the first parturition. - Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis
Hiroto Miura, Masayuki Takeda, Megumi Yamaguchi, Yoshihisa Ohtani, Go Endo, Yasuhisa Masuda, Kaede Ito, Yoshio Nagura, Kunihiro Iwashita, Tomohiro Mitani, Yutaka Suzuki, Yasuo Kobayashi, Satoshi Koike
Frontiers in Microbiology, 13, 783058, 783058, Frontiers Media SA, 2022年03月24日, ［国際誌］
英語, 研究論文（学術雑誌）, The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA gene. In the present study, we performed three experiments to establish a high-resolution and high-throughput rumen microbial profiling approach using a combination of MinION platform and buccal swab sample, which is a proxy for rumen contents. In experiment 1, rumen contents and buccal swab samples were collected simultaneously from cannulated cattle (n = 6) and used for microbiota analysis using three different analytical workflows: amplicon sequencing of the V3–V4 region of the 16S rRNA gene using MiSeq and amplicon sequencing of near full-length 16S rRNA gene using MinION or PacBio Sequel II. All reads derived from the MinION and PacBio platforms were classified at the species-level. In experiment 2, rumen fluid samples were collected from beef cattle (n = 28) and used for 16S rRNA gene amplicon sequencing using the MinION platform to evaluate this sequencing platform for rumen microbiota analysis. We confirmed that the MinION platform allowed species-level taxa assignment for the predominant bacterial groups, which were previously identified at the family- and genus-level using the MiSeq platform. In experiment 3, buccal swab samples were collected from beef cattle (n = 30) and used for 16S rRNA gene amplicon sequencing using the MinION platform to validate the applicability of a combination of the MinION platform and buccal swab samples for rumen microbiota analysis. The distribution of predominant bacterial taxa in the buccal swab samples was similar to that in the rumen samples observed in experiment 2. Based on these results, we concluded that the combination of the MinION platform and buccal swab samples may be potentially applied for rumen microbial analysis in large-scale studies. - Expression of chemerin in intestinal mucosa of calves with comparable expression level with other antimicrobial proteins
Yutaka Suzuki, Kanako Kubota, Satoshi Haga, Hideaki Hayashi, Mutsumi Oishi, Hiroto Miura, Sanggun Roh, Satoshi Koike, Yasuo Kobayashi
Animal Science Journal, 93, 1, e13750, Wiley, 2022年01月, ［国際誌］
英語, 研究論文（学術雑誌）, Neonatal calves are highly susceptible to infectious disorders including diarrhea. Therefore, epithelial innate immunity, including antimicrobial peptides/proteins (AMPs), is important during the early stage of their lives. Chemerin, a multifunctional protein that was originally identified as a chemokine, possesses a potent antimicrobial activity. The present study investigated the expression levels of chemerin in the gastrointestinal (GI) tract of growing calves. Chemerin and its coding gene, retinoic acid receptor responder protein 2 (RARRES2), were highly expressed in duodenum, jejunum, and ileum compared with other parts of the GI tract. Immunohistochemistry demonstrated that chemerin-producing cells were localized in the crypt of the intestinal mucosa. Finally, the expression level of RARRES2 was higher compared with those of other major AMPs in duodenum, although it was lower compared with that of enteric β-defensin but mostly higher than those of other AMPs in jejunum and ileum at various ages in calves. The expression levels of RARRES2 were not influenced by the age of calves in duodenum and jejunum, whereas a higher expression level of RARRES2 in ileum was observed in younger calves. This study revealed that chemerin is produced in the small intestine of calves and has the potential to contribute to the gut epithelial barrier system. - Accelerated discovery of novel glycoside hydrolases using targeted functional profiling and selective pressure on the rumen microbiome
André L. A. Neves, Jiangkun Yu, Yutaka Suzuki, Marisol Baez-Magana, Elena Arutyunova, Eóin O’Hara, Tim McAllister, Kim H. Ominski, M. Joanne Lemieux, Le Luo Guan
Microbiome, 9, 1, 229, 229, Springer Science and Business Media LLC, 2021年12月, ［国際誌］
英語, 研究論文（学術雑誌）, Abstract
Background
Carbohydrate-active enzymes (CAZymes) form the most widespread and structurally diverse set of enzymes involved in the breakdown, biosynthesis, or modification of lignocellulose that can be found in living organisms. However, the structural diversity of CAZymes has rendered the targeted discovery of novel enzymes extremely challenging, as these proteins catalyze many different chemical reactions and are sourced by a vast array of microbes. Consequently, many uncharacterized members of CAZyme families of interest have been overlooked by current methodologies (e.g., metagenomic screening) used to discover lignocellulolytic enzymes.
Results
In the present study, we combined phenotype-based selective pressure on the rumen microbiota with targeted functional profiling to guide the discovery of unknown CAZymes. In this study, we found 61 families of glycoside hydrolases (GH) (out of 182 CAZymes) from protein sequences deposited in the CAZy database—currently associated with more than 20,324 microbial genomes. Phenotype-based selective pressure on the rumen microbiome showed that lignocellulolytic bacteria (e.g., Fibrobacter succinogenes, Butyrivibrio proteoclasticus) and three GH families (e.g., GH11, GH13, GH45) exhibited an increased relative abundance in the rumen of feed efficient cattle when compared to their inefficient counterparts. These results paved the way for the application of targeted functional profiling to screen members of the GH11 and GH45 families against a de novo protein reference database comprised of 1184 uncharacterized enzymes, which led to the identification of 18 putative xylanases (GH11) and three putative endoglucanases (GH45). The biochemical proof of the xylanolytic activity of the newly discovered enzyme validated the computational simulations and demonstrated the stability of the most abundant xylanase.
Conclusions
These findings contribute to the discovery of novel enzymes for the breakdown, biosynthesis, or modification of lignocellulose and demonstrate that the rumen microbiome is a source of promising enzyme candidates for the biotechnology industry. The combined approaches conceptualized in this study can be adapted to any microbial environment, provided that the targeted microbiome is easy to manipulate and facilitates enrichment for the microbes of interest. - Addition of ginkgo fruit to cattle feces and slurry suppresses methane production by altering the microbial community structure
Risa Shintani, Seongjin Oh, Yutaka Suzuki, Satoshi Koike, Yasuo Kobayashi
ANIMAL SCIENCE JOURNAL, 92, 1, e13620, WILEY, 2021年12月, ［国際誌］
英語, 研究論文（学術雑誌）, The effect of ginkgo fruit addition on methane production potential of cattle feces and slurry was assessed in relation to other fermentation products and the microbial community. Holstein cattle fresh feces and slurry were left at 30 degrees C for 0, 30, 60, 90, and 180 days with/without ginkgo fruit to monitor the effect on fermentation potential. With the addition of ginkgo fruit, methane production potential of feces was reduced on Day 30 and thereafter, and that of slurry was consistently reduced over the experimental period. As a general trend, ginkgo fruit addition resulted in decreased acetate and increased propionate in feces and acetate accumulation in slurry. With ginkgo fruit addition, MiSeq analyses indicated decreases in methanogen (in particular Methanocorpusculum), Ruminococcaceae, and Clostridiaceae populations and increases in Bacteroidaceae and Porphyromonadaceae populations, which essentially agreed with quantitative real-time polymerase chain reaction (qPCR) assay results. These data indicate that direct addition of ginkgo fruit to cattle excreta is useful for reducing methane emissions by altering the microbial community structure. The application of ginkgo fruit to lower methane emissions from cattle excreta is, therefore, useful in cases in which the excreta is left without special management for a long period of time. - Identification of the core rumen bacterial taxa and their population dynamics during the fattening period in Japanese Black cattle
Hiroto Miura, Takuya Hashimoto, Yukiko Kawanishi, Hiroki Kawauchi, Ryo Inoue, Noriaki Shoji, Kunihiko Saito, Mario Sekiya, Yosuke Saito, Jumpei Yasuda, Chiemi Yonezawa, Tetsushiro Endo, Hirotaka Kasuya, Yutaka Suzuki, Yasuo Kobayashi, Satoshi Koike
ANIMAL SCIENCE JOURNAL, 92, 1, e13601, WILEY, 2021年12月, ［国際誌］
英語, 研究論文（学術雑誌）, The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. - Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows
Yutaka Suzuki, Sachi Chiba, Koki Nishihara, Keiichi Nakajima, Akihiko Hagino, Won-Seob Kim, Hong-Gu Lee, Tomonori Nochi, Toru Suzuki, Sang-Gun Roh
Animals, 11, 11, 3194, 3194, MDPI AG, 2021年11月09日, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Epithelial barrier function in the mammary gland acts as a forefront of the defense mechanism against mastitis, which is widespread and a major disorder in dairy production. Chemerin is a chemoattractant protein with potent antimicrobial ability, but its role in the mammary gland remains unelucidated. The aim of this study was to determine the function of chemerin in mammary epithelial tissue of dairy cows in lactation or dry-off periods. Mammary epithelial cells produced chemerin protein, and secreted chemerin was detected in milk samples. Chemerin treatment promoted the proliferation of cultured bovine mammary epithelial cells and protected the integrity of the epithelial cell layer from hydrogen peroxide (H2O2)-induced damage. Meanwhile, chemerin levels were higher in mammary tissue with mastitis. Tumor necrosis factor alpha (TNF-α) strongly upregulated the expression of the chemerin-coding gene (RARRES2) in mammary epithelial cells. Therefore, chemerin was suggested to support mammary epithelial cell growth and epithelial barrier function and to be regulated by inflammatory stimuli. Our results may indicate chemerin as a novel therapeutic target for diseases in the bovine mammary gland. - Effects of oral administration of timothy hay and psyllium on the growth performance and fecal microbiota of preweaning calves
K.A.H.T. Kodithuwakku, H. Owada, H. Miura, D. Maruyama, K. Hirano, Y. Suzuki, Y. Kobayashi, S. Koike
Journal of Dairy Science, American Dairy Science Association, 2021年09月
研究論文（学術雑誌） - Chemical and microbial characterization for fermentation of water-soluble cellulose acetate in human stool cultures
Hiroaki Yamada, Yuto Watabe, Yutaka Suzuki, Satoshi Koike, Shu Shimamoto, Yasuo Kobayashi
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 101, 7, 2950, 2960, WILEY, 2021年05月, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND Water-soluble cellulose acetate (WSCA), a synthetic fiber source, was applied to human stool cultures and to pure cultures of representative Bacteroides species to characterize the fermentation properties of WSCA in the human gut, and to assess the potential availability of WSCA as a food or additive candidate.RESULTS All nine of the different types of WSCA tested here provided increased acetate levels in human stool cultures. Greater levels of deacetylation were observed as the degree of substitution of hydroxyl groups by acetyl groups decreased. Among the nine tested types of WSCA, CA-0.78-128 caused the largest shifts of the microbial community, including an increased abundance of members of the genus Bacteroides, especially Bacteroides uniformis. Of four representative human gut Bacteroides species, only B. uniformis grew in pure culture on WSCA to produce acetate actively.CONCLUSION Water-soluble cellulose acetate has the potential for dietary application in human and other monogastric animals, based on the enhanced production of short-chain fatty acids (SCFAs), in particular acetate, in the hindgut. Short-chain fatty acid production is caused by selective proliferation of specific gut bacteria belonging to the genus Bacteroides. (c) 2020 Society of Chemical Industry - Cashew nut shell liquid potentially mitigates methane emission from the feces of Thai native ruminant livestock by modifying fecal microbiota
Chiaki Narabe, Saki Kamiyama, Mizuki Saito, Phoompong Boonsaen, Anchalee Khongpradit, Suriya Sawanon, Yutaka Suzuki, Satoshi Koike, Yasuo Kobayashi
Animal Science Journal, 92, 1, e13614, Wiley, 2021年01月, ［国際誌］
英語, 研究論文（学術雑誌）, The methane-mitigating potency of cashew nutshell liquid (CNSL) was evaluated by investigating gas production from batch cultures using feces from Thai native ruminants that had been incubated for different periods. Feces was obtained from four Thai native cattle and four swamp buffaloes reared under practical feeding conditions at the Kasetsart University farm, Thailand. Fecal slurry from the same farm was also included in the analysis. CNSL addition successfully suppressed the methane production potential of feces from both ruminants by shifting short chain fatty acid profiles towards propionate production. Methane mitigation continued for almost 150 days, although the degree of mitigation was more apparent from Day 0 to Day 30. Bacterial and archaeal community shifts with CNSL addition were observed in feces from both ruminants; specifically, Bacteroides increased, whereas Lachnospiraceae and Ruminococcaceae decreased in feces to which CNSL was added. Fecal slurry did not show marked changes in gas production with CNSL addition. The findings showed that the addition of CNSL to the feces of ruminants native to the Southeast Asian region can suppress methane emission. Because CNSL can be easily obtained as a byproduct of the local cashew industry in this region, its on-site application might be ideal. - Effect of trehalose supplementation in milk replacer on the incidence of diarrhea and fecal microbiota in preweaned calves
Hiroto Miura, Kazuhisa Mukai, Keigo Sudo, Satoshi Haga, Yutaka Suzuki, Yasuo Kobayashi, Satoshi Koike
Journal of Animal Science, 99, 1, Oxford University Press (OUP), 2021年01月01日, ［国際誌］
英語, 研究論文（学術雑誌）,Abstract
Trehalose, a nonreducing disaccharide consisting of d-glucose with α,α-1,1 linkage, was evaluated as a functional material to improve the gut environment in preweaned calves. In experiment 1, 173 calves were divided into two groups; the trehalose group was fed trehalose at 30 g/animal/d with milk replacer during the suckling period, and the control group was fed nonsupplemented milk replacer. Medication frequency was lower in the trehalose group (P < 0.05). In experiment 2, calves (n = 20) were divided into two groups (control group [n = 10] and trehalose group [n = 10]) based on their body weight and reared under the same feeding regimens as in experiment 1. Fresh feces were collected from individual animals at the beginning of the trial (average age 11 d), 3 wk after trehalose feeding (experimental day 22), and 1 d before weaning, and the fecal score was recorded daily. Fecal samples were analyzed for fermentation parameters and microbiota. The fecal score was significantly lower in the trehalose group than in the control group in the early stage (at an age of 14 to 18 d; P < 0.05) of the suckling period. Calves fed trehalose tended to have a higher proportion of fecal butyrate on day 22 than calves in the control group (P = 0.08). Population sizes of Clostridium spp. were significantly lower (P = 0.036), whereas those of Dialister spp. and Eubacterium spp. tended to be higher in the feces of calves in the trehalose group on day 22 (P = 0.060 and P = 0.083). These observations indicate that trehalose feeding modulated the gut environment and partially contributed to the reduction in medication frequency observed in experiment 1. - Effect of colostrum feeding strategies on the expression of neuroendocrine genes and active gut mucosa-attached bacterial populations in neonatal calves.
Jitka Hromádková, Yutaka Suzuki, Sarah Pletts, Jade Pyo, Tao Ma, Yanhong Chen, Michael A Steele, Le Luo Guan
Journal of dairy science, 103, 9, 8629, 8642, 2020年09月, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Colostrum feeding is vital for the development of the immune system and gastrointestinal tract in neonatal calves; however, it is currently unknown whether different colostrum feeding strategies affect their neuroendocrine system and potentially the gut-brain axis. The present study investigated the effect of 3 different colostrum feeding regimens on the expression of neuroendocrine genes in adrenal glands and gastrointestinal tissues and on the abundance of intestinal commensal bacteria. Holstein bull calves were fed colostrum immediately after birth and randomly assigned to 3 groups: whole milk (n = 8), mixture of 50% colostrum and 50% whole milk (n = 8), and colostrum (CF; n = 8) for 72 h with 12-h intervals. Adrenal glands, ileum, and colon tissues were collected at 75 h and were subjected to the expression of 11 targeted neuroendocrine genes and the abundance of tissue mucosa-associated bacteria measurement using quantitative real-time PCR and quantitative PCR, respectively. The expressions of all targeted genes were detected, and the expression of α-adrenergic receptor (ADRA1A) gene was affected by CF in adrenal glands and gut tissues. In addition, CF upregulated the expression of HTR4 (serotonin receptor) and SLC4A4 (serotonin transporter) genes in the ileum and increased the abundance of active Lactobacillus spp. and Escherichia coli (as detected at RNA level) associated with ileum and colon tissue. Furthermore, there were positive correlations between the abundance of active Lactobacillus spp. and E. coli with expression of HTR2B and HTR4 genes in the colon, suggesting that extended colostrum feeding strategies may affect the interaction between gut microbiota and host endocrine functions in neonatal calves. - Ruminal epithelial insulin‐like growth factor‐binding proteins 2, 3, and 6 are associated with epithelial cell proliferation
Koki Nishihara, Yutaka Suzuki, Sanggun Roh
Animal Science Journal, 91, 1, e13422, Wiley, 2020年01月, ［国際誌］
英語, 研究論文（学術雑誌）, The aim of this study was to identify factors that regulate ruminal epithelial insulin-like growth factor-binding protein (IGFBP) expression and determine its role in rumen epithelial cell proliferation. Primary bovine rumen epithelial cells (BREC) were incubated with short-chain fatty acids (SCFAs) at pH 7.4 or 5.6, lactate, lipopolysaccharide (LPS), insulin-like growth factor-I (IGF-I), -II (IGF-II), or recombinant bovine IGFBP2 (rbIGFBP2). The mRNA expression levels of IGFBP in BREC were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation rate of BREC was analyzed using a WST-1 assay. IGFBP2 gene expression tended to be lower with SCFA treatment (p < .1), and IGFBP6 gene expression was significantly lower with SCFA treatment (p < .05). IGFBP3 and IGFBP6 gene expression tended to be higher with d-Lactate treatment (p < .1). IGFBP3 gene expression was significantly higher (p < .05) with LPS treatment. BREC treated with IGF-I grew more rapidly than vehicle control-treated cells (p < .01); however, recombinant bovine rbIGFBP2 inhibited IGF-I-induced proliferation. IGF-II and/or rbIGFBP2 did not affect BREC proliferation. Taken together, SCFA treatment decreased IGFBP2 and IGFBP6 expression in rumen epithelial cells, and lower expression of these IGFBP might promote rumen epithelial cell proliferation by facilitating IGF-I. - Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.
Anchalee Khongpradit, Phoompong Boonsaen, Nitipong Homwong, Yutaka Suzuki, Satoshi Koike, Suriya Sawanon, Yasuo Kobayashi
Animal science journal = Nihon chikusan Gakkaiho, 91, 1, e13459, 2020年01月, ［国際誌］
英語, 研究論文（学術雑誌）, Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study. PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level. PS feeding improved weight gain and feed conversion ratio without affecting feed intake. PS did not obviously influence blood lipid profiles throughout the experiment. Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study. Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment. These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance. - Selection of plant oil as a supplemental energy source by monitoring rumen profiles and its dietary application in Thai crossbred beef cattle.
Keiji Matsuba, Apirada Padlom, Anchalee Khongpradit, Phoompong Boonsaen, Prayad Thirawong, Suriya Sawanon, Yutaka Suzuki, Satoshi Koike, Yasuo Kobayashi
Asian-Australasian journal of animal sciences, 32, 10, 1511, 1520, 2019年10月, ［国際誌］
英語, 研究論文（学術雑誌）, Objective: The present study was conducted to select a plant oil without inhibitory effects on rumen fermentation and microbes, and to determine the optimal supplementation level of the selected oil in a series of in vitro studies for dietary application. Then, the selected oil was evaluated in a feeding study using Thai crossbred beef cattle by monitoring growth, carcass, blood and rumen characteristics. Methods: Rumen fluid was incubated with substrates containing one of three different types of plant oil (coconut oil, palm oil and soybean oil) widely available in Thailand. The effects of each oil on rumen fermentation and microbes were monitored and the oil without a negative influence on rumen parameters was selected. Then, the dose-response of rumen parameters to various levels of the selected palm oil was monitored to determine a suitable supplementation level. Finally, an 8-month feeding experiment with the diet supplemented with palm oil was carried out using 12 Thai crossbred beef cattle to monitor growth, carcass, rumen and blood profiles. Results: Batch culture studies revealed that coconut and soybean oils inhibited the most potent rumen cellulolytic bacterium Fibrobacter succinogenes, while palm oil had no such negative effect on this and on rumen fermentation products at 5% or higher supplementation level. Cattle fed the diet supplemented with 2.5% palm oil showed improved feed conversion ratio (FCR) without any adverse effects on rumen fermentation. Palm oil-supplemented diet increased blood cholesterol levels, suggesting a higher energy status of the experimental cattle. Conclusion: Palm oil had no negative effects on rumen fermentation and microbes when supplemented at levels up to 5% in vitro. Thai crossbred cattle fed the palm oil-supplemented diet showed improved FCR without apparent changes of rumen and carcass characteristics, but with elevated blood cholesterol levels. Therefore, palm oil can be used as a beneficial energy source. - Growth of rumen papillae in weaned calves is associated with lower expression of insulin-like growth factor-binding proteins 2, 3, and 6.
Koki Nishihara, Yutaka Suzuki, Dahye Kim, Sanggun Roh
Animal science journal = Nihon chikusan Gakkaiho, 90, 9, 1287, 1292, 2019年09月, ［国際誌］
英語, 研究論文（学術雑誌）, This study aimed to characterize the relationship between the growth of rumen papillae in calves and the mRNA expression of insulin-like growth factor-binding proteins (IGFBPs) in the rumen papillae. The length of rumen papillae, the mRNA expression of IGFBPs in rumen papillae by quantitative real-time PCR, and the presence of insulin-like growth factors I and II (IGF-I and II) by immunohistochemistry (IHC) were analyzed in nine Holstein calves divided into three groups: suckling (2 weeks, n = 3), milk-continued (8 weeks, n = 3), and weaned (8 weeks, n = 3). The length of rumen papillae was greater (p < 0.01) in weaned calves than in suckling and milk-continued calves, whereas the expressions of IGFBP2, IGFBP3, and IGFBP6 genes were lower (p < 0.05) in the rumen papillae of weaned calves than in milk-continued calves. Thus, rumen papillae length and IGFBP2, 3, and 6 expressions were negatively correlated. The IHC analysis showed that IGF-I and IGF-II were present in the rumen epithelium of calves. These results suggested that the growth of rumen papillae after weaning is associated with the induction of IGFs by the low levels of IGFBP2, IGFBP3, and IGFBP6. - Effect of cashew nut shell liquid feeding on fermentation and microbiota in the rumen of Thai native cattle and swamp buffaloes
S. Konda, R. Onodera, E. Kanchanasatit, P. Boonsaen, S. Sawanon, K. Nagashima, Y. Suzuki, S. Koike, Y. Kobayashi
Livestock Science, 226, 99, 106, Elsevier BV, 2019年08月
研究論文（学術雑誌） - Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter.
Hidehisa Yamano, Yasuhiro Ichimura, Yoshihiko Sawabe, Satoshi Koike, Yutaka Suzuki, Yasuo Kobayashi
Animal science journal = Nihon chikusan Gakkaiho, 90, 6, 790, 798, 2019年06月, ［国際誌］
英語, 研究論文（学術雑誌）, Rumen digesta was obtained from wild Hokkaido sika deer to compare bacterial flora between summer and winter. Bacterial flora was characterized with molecular-based approaches and enrichment cultivation. Bacteroidetes was shown as a major phylum followed by Firmicutes, with similar proportions in both seasons. However, two phylogenetically unique groups in Bacteroidetes were found in each season: unknown group A in winter and unknown group B in summer. The ruminal abundance of unknown group A was the highest followed by Ruminococcus flavefaciens in winter. Moreover, the abundance of these two was higher in winter than in summer. In contrast, the abundance of unknown group B was higher in summer than in winter. In addition, this group showed the highest abundance in summer among the bacteria quantified. Unknown group A was successfully enriched by cultivating with oak bark and sterilized rumen fluid, particularly that from deer. Bacteria of this group were distributed in association with the solid rather than the liquid rumen fraction, and were detected as small cocci. Accordingly, unknown group A is assumed to be involved in degradation of fibrous materials. These results suggest that wild Hokkaido sika deer develop a rumen bacterial flora in response to changes in dietary conditions. - Cellulose acetate, a new candidate feed supplement for ruminant animals: In vitro evaluations
Y. Watabe, Y. Suzuki, S. Koike, S. Shimamoto, Y. Kobayashi
Journal of Dairy Science, 101, 12, 10929, 10938, American Dairy Science Association, 2018年12月
研究論文（学術雑誌） - Dissect the mode of action of probiotics in affecting host-microbial interactions and immunity in food producing animals
Tao Ma, Yutaka Suzuki, Le Luo Guan
Veterinary Immunology and Immunopathology, 205, 35, 48, Elsevier BV, 2018年11月
研究論文（学術雑誌） - Rumen responses to dietary supplementation with cashew nut shell liquid and its cessation in sheep.
Kang S, Suzuki R, Suzuki Y, Koike S, Nagashima K, Kobayashi Y
Animal science journal = Nihon chikusan Gakkaiho, 89, 11, 1549, 1555, Wiley, 2018年09月
研究論文（学術雑誌） - Downregulated angiopoietin-like protein 8 production at calving related to changes in lipid metabolism in dairy cows.
Misato Nakano, Yutaka Suzuki, Satoshi Haga, Eri Yamauchi, Dahye Kim, Koki Nishihara, Keiichi Nakajima, Takafumi Gotoh, Seungju Park, Myunggi Baik, Kazuo Katoh, Sanggun Roh
Journal of animal science, 96, 7, 2646, 2658, 2018年06月29日, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Acute physiological adaptation of lipid metabolism during the postpartum transition period of cows facilitates peripheral metabolic regulation. Hepatokines, which are hormones secreted from hepatocytes, are presumed to play a critical role in systemic metabolic regulation. Angiopoietin-like protein 8 (ANGPTL8) has been identified as a novel hepatokine associated with circulating triglyceride concentrations in mice and humans. However, regulation of ANGPTL8 and its physiological effects is still unknown in cattle. The present study aimed to reveal changes in ANGPTL8 expression and secretion during the periparturient period, and to investigate its regulatory effect on adipocytes and mammary epithelial cells. In the peripartum period, liver ANGPTL8 mRNA expression was lesser on the day of parturition and 1 wk postpartum than it was 1 wk before parturition (P < 0.05). Moreover, plasma ANGPTL8 concentrations decreased on the day of parturition as compared with that 1 wk before parturition (P < 0.05). In addition, ANGPTL8 expression in cultured bovine hepatocytes was downregulated after oleate and palmitate treatment but upregulated after insulin treatment (P < 0.05). ANGPTL8 decreased hormone-sensitive lipase (HSL) expression in differentiated adipocytes and cluster of differentiation 36 (CD36), fatty acid synthase (FAS), acetyl-coa carboxylase (ACC), and stearoyl-coa desaturase (SCD) in cultured bovine mammary epithelial cells (P < 0.05). These data suggest that hepatic ANGPTL8 production was downregulated postpartum when the cows experienced a negative energy balance. This downregulation was associated with increased concentrations of NEFA and decreased concentrations of insulin in lactating cows, and it facilitated lipid mobilization from adipose tissue to the mammary glands. We speculate that ANGPTL8 might have beneficial effects in reverting or improving the physiological adaptation and pathological processes of lipid metabolism during the peripartum period. - Comparative transcriptome analysis of rumen papillae in suckling and weaned Japanese Black calves using RNA sequencing.
Koki Nishihara, Daichi Kato, Yutaka Suzuki, Dahye Kim, Misato Nakano, Yu Yajima, Satoshi Haga, Miwa Nakano, Hiroshi Ishizaki, Ryouka Kawahara-Miki, Tomohiro Kono, Kazuo Katoh, Sang-Gun Roh
Journal of animal science, 96, 6, 2226, 2237, 2018年06月04日, ［国際誌］
英語, 研究論文（学術雑誌）, The length and density of rumen papillae starts to increase during weaning and growth of ruminants. This significant development increases the intraruminal surface area and the efficiency of VFA (acetate, propionate, butyrate, etc.) uptake. Thus, it is important to investigate the factors controlling the growth and development of rumen papillae during weaning. This study aimed to compare the transcriptomes of rumen papillae in suckling and weaned calves. Total RNA was extracted from the rumen papillae of 10 male Japanese Black calves (5 suckling calves, 5 wk old; 5 weaned calves, 15 wk old) and used in RNA-sequencing. Transcript abundance was estimated and differentially expressed genes were identified and these data were then used in Ingenuity Pathway Analysis (IPA) to predict the major canonical pathways and upstream regulators. Among the 871 differentially expressed genes screened by IPA, 466 genes were upregulated and 405 were downregulated in the weaned group. Canonical pathway analysis showed that "atherosclerosis" was the most significant pathway, and "tretinoin," a derivative of vitamin A, was predicted as the most active upstream regulator during weaning. Analyses also predicted IgG, lipopolysaccharides, and tumor-necrosis factor-α as regulators of the microbe-epithelium interaction that activates rumen-related immune responses. The functional category and the up-regulators found in this study provide a valuable resource for studying new candidate genes related to the proliferation and development of rumen papillae from suckling to weaning Japanese Black calves. - Partial characterization of phylogeny, ecology and function of the fibrolytic bacterium Ruminococcus flavefaciens OS14, newly isolated from the rumen of swamp buffalo.
Boonsaen P, Kinjo M, Sawanon S, Suzuki Y, Koike S, Kobayashi Y
Animal science journal = Nihon chikusan Gakkaiho, 89, 2, 377, 385, Blackwell Publishing, 2018年02月01日
英語, 研究論文（学術雑誌）, The fibrolytic rumen bacterium Ruminococcus flavefaciensOS14 was isolated from swamp buffalo and its phylogenetic, ecological and digestive properties were partially characterized. Isolates from rumen contents of four swamp buffalo were screened for fibrolytic bacteria
one of the 40 isolates showed a distinctive feature of solubilizing cellulose powder in liquid culture and was identified as R. flavefaciens based on its 16S ribosomal DNA sequence. This isolate, OS14, was employed for detection and digestion studies, for which a quantitative PCR assay was developed and defined cultures were tested with representative forages in Thailand. OS14 was phylogenetically distant from other isolated and uncultured R. flavefaciens and showed limited distribution among Thai ruminants but was absent in Japanese cattle. OS14 digested rice straw and other tropical forage to a greater extent than the type strain C94 of R. flavefaciens. OS14 produced more lactate than C94, and digested para grass to produce propionate more extensively in co-culture with lactate-utilizing Selenomonas ruminantium S137 than a co-culture of C94 with S137. These results indicate that phylogenetically distinct OS14 could digest Thai local forage more efficiently than the type strain, possibly forming a symbiotic cross-feeding relationship with lactate-utilizing bacteria. This strain might be useful for future animal and other industrial applications. - Post-prandial decrease in plasma growth hormone levels is not related to the increase in plasma insulin levels in goats
Koki Nishihara, Ryoko Kobayashi, Yutaka Suzuki, Katsuyoshi Sato, Kazuo Katoh, Sanggun Roh
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 30, 12, 1696, 1701, ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2017年12月
英語, 研究論文（学術雑誌）, Objective: In the present study, we examined whether the post-prandial reduction in plasma growth hormone (GH) levels is related to the increase in plasma insulin levels in ruminants.
Methods: We performed two experiments: intravenous bolus injection of insulin (0.2 IU/kg body weight) or glucose (1.0 mmol/kg body weight) was administered to increase the plasma insulin levels in male Shiba goats.
Results: In the insulin injection experiment, significant (p<0.05) increase in GH concentrations was observed, 15 to 20 min after the injection; it was accompanied with a significant (p<0.01) increase in cortisol concentrations at 45 to 90 min, when compared to the concentrations in the saline-injected controls. The glucose injection significantly (p<0.05) increased the plasma GH concentration at 20 to 45 min; this was not accompanied by significantly higher cortisol concentrations than were observed for the saline-injected control. Hypoglycemia induced by the insulin injection, which causes the excitation of the adrenal cortex, might be involved in the increase in insulin levels.
Conclusion: Based on these results, we conclude that post-prandial increases in plasma insulin or glucose levels do not induce a decrease in GH concentration after feeding in the ruminants. - Potency of ginkgo fruit for modulation of rumen microbiota and fermentation
OH Seongjin, SUZUKI Yutaka, KOIKE Satoshi, KOBAYASHI Yasuo
栄養生理研究会報, 61, 2, 33‐41, 41, 家畜栄養生理研究会, 2017年09月
英語 - Bean husk, an agricultural by-product having functionality in animal nutrition and health
MYINT Htun, KISHI Hiroyuki, IWAHASHI Yu, SUZUKI Yutaka, KOIKE Satoshi, KOBAYASHI Yasuo
栄養生理研究会報, 61, 2, 13‐19, 19, 家畜栄養生理研究会, 2017年09月
英語 - Epigallocatechin-3-gallate increases autophagy signaling in resting and unloaded plantaris muscles but selectively suppresses autophagy protein abundance in reloaded muscles of aged rats
Hideyuki Takahashi, Yutaka Suzuki, Junaith S. Mohamed, Takafumi Gotoh, Suzette L. Pereira, Stephen E. Alway
EXPERIMENTAL GERONTOLOGY, 92, 56, 66, PERGAMON-ELSEVIER SCIENCE LTD, 2017年06月
英語, 研究論文（学術雑誌）, We have previously found that Epigallocatechin-3-gallate (EGCg), an abundant catechin in green tea, reduced apoptotic signaling and improved muscle recovery in response to reloading after hindlimb suspension (HS). In this study, we investigated if EGCg altered autophagy signaling in skeletal muscle of old rats in response to HS or reloading after HS. Fischer 344 x Brown Norway inbred rats (age 34 months) were given 1 ml/day of purified EGCg (50 mg/kg body weight), or the same sample volume of the vehicle by gavage. One group of animals received HS for 14 days and the second group of rats received 14 days of HS, then the HS was removed and they were allowed to recover by ambulating normally around the cage for two weeks. EGCg decreased a small number of autophagy genes in control muscles, but it increased the expression of other autophagy genes (e.g., ATG16L2, SNCA, TM9SF1, Pink1, PIM-2) and HS did not attenuate these increases. HS increased Beclinl, ATG7 and LC3-II/I protein abundance in hindlimb muscles. Relative to vehicle treatment, EGCg treatment had greater ATG12 protein abundance (35.8%, P < 0.05), but decreased Beclinl protein levels (-101.1%, P < 0.05) after HS. However, in reloaded muscles, EGCg suppressed Beclinl and LC3-II/I protein abundance as compared to vehicle treated muscles. EGCg appeared to "prime" autophagy signaling before and enhance autophagy gene expression and protein levels during unloading in muscles of aged rats, perhaps to improve the clearance of damaged organelles. However, EGCg suppressed autophagy signaling after reloading, potentially to increase the recovery of hindlimb muscles mass and function after loading is restored. (C) 2017 Elsevier Inc. All rights reserved. - ウシ組織におけるα‐トコフェロール体内動態関連遺伝子の発現特性に関する研究
芳賀聡, 中野美和, 宮地慎, 石崎宏, 松山裕城, 鈴木裕, 鈴木裕, 北山峻, 小林洋介, 加藤和雄, 盧尚建
栄養生理研究会報, 61, 1, 9‐19, 19, 家畜栄養生理研究会, 2017年03月
日本語 - Potency of cashew nut shell liquid in rumen modulation under different dietary conditions and indication of its surfactant action against rumen bacteria.
Oh S, Suzuki Y, Hayashi S, Suzuki Y, Koike S, Kobayashi Y
Journal of animal science and technology, 59, 1, 27, Springer Science and Business Media LLC, 2017年
研究論文（学術雑誌） - Postweaning changes in the expression of chemerin and its receptors in calves are associated with the modification of glucose metabolism
Y. Suzuki, S. Haga, M. Nakano, H. Ishizaki, M. Nakano, S. Song, K. Katoh, S. Roh
JOURNAL OF ANIMAL SCIENCE, 94, 11, 4600, 4610, AMER SOC ANIMAL SCIENCE, 2016年11月, ［筆頭著者］
英語, 研究論文（学術雑誌）, Chemerin, originally known as a chemoattractant derived from adipose tissue and the liver, has been reported to have regulatory functions in gluconeogenesis, peripheral insulin sensitivity, and insulin secretion. This study was conducted to assess the postweaning changes in expression of this cytokine and its physiological role in the modification of glucose metabolism associated with weaning. Eighteen tissue samples were collected from Holstein calves (90 d of age; n = 4) to investigate the tissue distributions of chemerin and its receptors genes. Chemerin was highly expressed in the liver, and secreted chemerin protein was found in the plasma. Among the receptors of chemerin, chemokine-like receptor 1 and C-C chemokine receptor-like 2 were ubiquitously expressed whereas G protein-coupled receptor 1 was predominantly expressed in the liver. The changes in glucose metabolism and expression of these genes after weaning were assessed by comparing suckling calves (n = 6) and weaned calves (n = 8) of Japanese Black cattle. No significant difference was observed in plasma glucose levels between suckling and weaned calves (P = 0.22), whereas the plasma level of total ketone bodies was significantly higher in weaned calves (P < 0.01). Plasma levels of insulin and cortisol did not differ between suckling and weaned calves. The mRNA levels of certain key enzymes involved in hepatic gluconeogenesis were also altered; for instance, pyruvate carboxylase level was lower in postweaning calves (P < 0.05) and phosphoenolpyruvate carboxykinase 2 (PCK2) level tended to be higher after weaning (P = 0.08). However, PCK1 was not altered after weaning. The plasma levels of hepatic stress indicators were also changed, with aspartate transaminase, alanine transaminase, and lactate dehydrogenase being significantly elevated in postweaning calves (P < 0.05). Chemerin protein in liver tissue was less abundant in weaned calves (P < 0.05), although there were no changes in its transcript levels. The abundance of plasma chemerin protein did not change after weaning (P = 0.95). In summary, these data indicate that as a consequence of weaning, which causes physiological stress and alters hepatic metabolism, chemerin protein expression within the liver is downregulated, indicating that chemerin plays a role in the upregulation of hepatic PCK2 expression via its inhibitory effect on hepatic gluconeogenesis. - 離乳前後の仔牛のルーメン絨毛上皮組織における遺伝子発現量の比較
Roh Sanggun, 加藤 大地, 鈴木 裕, 芳賀 聡
栄養生理研究会報 = Proceedings of Japanese Society for Animal Nutrition and Metabolism, 60, 2, 79, 84, 家畜栄養生理研究会, 2016年07月
英語 - Utilization of digital differential display to identify differentially expressed genes related to rumen development
Daichi Kato, Yutaka Suzuki, Satoshi Haga, KyoungHa So, Eri Yamauchi, Miwa Nakano, Hiroshi Ishizaki, Kichoon Choi, Kazuo Katoh, Sang-Gun Roh
ANIMAL SCIENCE JOURNAL, 87, 4, 584, 590, WILEY-BLACKWELL, 2016年04月, ［筆頭著者］
英語, 研究論文（学術雑誌）, This study aimed to identify the genes associated with the development of the rumen epithelium by screening for candidate genes by digital differential display (DDD) in silico. Using DDD in NCBI's UniGene database, expressed sequence tag (EST)-based gene expression profiles were analyzed in rumen, reticulum, omasum, abomasum and other tissues in cattle. One hundred and ten candidate genes with high expression in the rumen were derived from a library of all tissues. The expression levels of 11 genes in all candidate genes were analyzed in the rumen, reticulum, omasum and abomasum of nine Japanese Black male calves (5-week-old pre-weaning: n=3; 15-week-old weaned calves: n=6). Among the 11 genes, only 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), aldo-keto reductase family 1, member C1-like (AKR1C1), and fatty acid binding protein 3 (FABP3) showed significant changes in the levels of gene expression in the rumen between the pre- and post-weaning of calves. These results indicate that DDD analysis in silico can be useful for screening candidate genes related to rumen development, and that the changes in expression levels of three genes in the rumen may have been caused by weaning, aging or both. (c) 2015 Japanese Society of Animal Science - 培養脂肪細胞におけるヨード高含有卵の卵黄の脂肪蓄積抑制の効果
Jiro YOKOYAMA, Kyoung-Ha SO, Yutaka SUZUKI, Megumi MORIOKA, Keiichi SUZUKI, Kazuo KATOH, Sang-gun ROH
Nihon Chikusan Gakkaiho, 87, 4, 345, 350, Japanese Society of Animal Science, 2016年
研究論文（学術雑誌） - Physiological Roles of Adipokines, Hepatokines, and Myokines in Ruminants
Sang-Gun Roh, Yutaka Suzuki, Takafumi Gotoh, Ryuichi Tatsumi, Kazuo Katoh
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 29, 1, 1, 15, ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2016年01月, ［筆頭著者］
英語, Since the discovery of leptin secreted from adipocytes, specialized tissues and cells have been found that secrete the several peptides (or cytokines) that are characterized to negatively and positively regulate the metabolic process. Different types of adipokines, hepatokines, and myokines, which act as cytokines, are secreted from adipose, liver, and muscle tissue, respectively, and have been identified and examined for their physiological roles in humans and disease in animal models. Recently, various studies of these cytokines have been conducted in ruminants, including dairy cattle, beef cattle, sheep, and goat. Interestingly, a few cytokines from these tissues in ruminants play an important role in the post-parturition, lactation, and fattening (marbling) periods. Thus, understanding these hormones is important for improving nutritional management in dairy cows and beef cattle. However, to our knowledge, there have been no reviews of the characteristics of these cytokines in beef and dairy products in ruminants. In particular, lipid and glucose metabolism in adipose tissue, liver tissue, and muscle tissue are very important for energy storage, production, and synthesis, which are regulated by these cytokines in ruminant production. In this review, we summarize the physiological roles of adipokines, hepatokines, and myokines in ruminants. This discussion provides a foundation for understanding the role of cytokines in animal production of ruminants. - 反芻動物におけるヘパトカインとしてのChemerinとANGPTL8の発現調節
鈴木裕, 中野美智, 芳賀聡, 芳賀聡, 中島恵一, 加藤和雄, ROH Sanggun
栄養生理研究会報, 59, 2, 59, 68, 家畜栄養生理研究会, 2015年10月, ［筆頭著者］
日本語 - Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells
Yutaka Suzuki, Satoshi Haga, Daiki Katoh, Kyoung-ha So, Ki-choon Choi, U-suk Jung, Hong-gu Lee, Kazuo Katoh, Sang-gun Roh
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 466, 3, 283, 288, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2015年10月, ［筆頭著者］
英語, 研究論文（学術雑誌）, Chemerin is a chemoattractant cytokine (chemokine) produced by adipocytes and hepatocytes; it regulates insulin sensitivity and adipocyte differentiation. The objective of this study was to investigate the effect of chemerin on the expression of genes related to lactogenesis and the regulators of chemerin signaling in a bovine mammary epithelial cell line (MAC-T). Two types of chemerin receptors, chemokine like-receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2), were detected in cultured MAC-T cells, whereas chemerin was not detected. G protein-coupled receptor 1 (GPR1), another receptor of chemerin, was undetectable in MAC-T cells. Chemerin upregulated transcript expression of CMKLR1, CCRL2, and genes associated with fatty acid synthesis, glucose uptake, insulin signaling, and casein synthesis in MAC-T cells. Lactogenic hormones (insulin, growth hormone, and prolactin) downregulated the expression of CMKLR1 in MAC-T cells. Adiponectin suppressed CMKLR1 expression. TNF-alpha suppressed CMKLRI, but induced CCRL2 expression. These data suggest chemerin is a novel regulator of lactogenesis via its own receptor in bovine mammary epithelial cells. (C) 2015 Elsevier Inc. All rights reserved. - Single Nucleotide Polymorphism in the Coding Region of Bovine Chemerin Gene and Their Associations with Carcass Traits in Japanese Black Cattle
Eri Yamauchi, Yutaka Suzuki, Kyoung-Ha So, Kei-ichi Suzuki, Kazuo Katoh, Sang-Gun Roh
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 28, 8, 1084, 1089, ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2015年08月
英語, 研究論文（学術雑誌）, Chemerin, highly expressed in adipose and liver tissues, regulates glucose and lipid metabolism and immunity in these tissues in ruminants and mice. Our previous reports showed that chemerin is involved in adipogenesis and lipid metabolism in adipose tissue as an adipokine. The aim of the present study was to identify single nucleotide polymorphisms (SNPs) in the coding region of the chemerin gene and to analyze their effects on carcass traits and intramuscular fatty acid compositions in Japanese Black cattle. The SNPs in the bovine chemerin gene were detected in 232 Japanese Black steers (n = 161) and heifers (n = 71) using DNA sequencing. The results revealed five novel silent mutations: NM_001046020: c.12A>G (4aa), c.165G<A (55aa), c.276C>T (92aa), c.321 A>G (107aa), and c.396C>T (132aa). There was no association between 4 of the SNPs (c.12A>G [4aa], c.165G<A [55aa], c.321 A>G [107aa], and c.396C>T) and carcass traits or intramuscular fatty acid compositions. Regarding the remaining SNP, c.276C>T, we found that cattle with genotype CC had a higher beef marbling score than that of cattle with genotype CT, whereas cattle with genotype CT had a higher body condition score (p<0.10). Further, cattle with genotype CC had significantly higher C18:0 content in their intramuscular fat tissue than that of cattle with genotype CT (p<0.05). On the other hand, cattle with genotype CT had significantly higher C14:0 and C16:0 content in their intramuscular fat tissue (p<0.05). Moreover, the number of individuals carrying the minor allele of c.276C>T SNP is small. It is suggested that the c.276C>T SNP of the chemerin gene has potential in cattle breeding using modern methods, such as marker assisted selection. So, further functional and physiological research elucidating the impact of the chemerin gene on bovine lipid metabolism including fatty acid synthesis will help in understanding these results. - Soluble extract of soybean fermented with Aspergillus oryzae GB 107 inhibits fat accumulation in cultured 3T3-L1 adipocytes
Kyoung-Ha So, Yasuki Suzuki, Shinichi Yonekura, Yutaka Suzuki, Chan Ho Lee, Sung Woo Kim, Kazuo Katoh, Sang-Gun Roh
NUTRITION RESEARCH AND PRACTICE, 9, 4, 439, 444, KOREAN NUTRITION SOC, 2015年08月
英語, 研究論文（学術雑誌）, BACKGROUND/OBJECTIVES: This study was conducted to investigate the effects of fermented soybean (FS) extract on adipocyte differentiation and fat accumulation using cultured 3T3-L1 adipocytes.
MATERIALS/METHODS: 3T3-L1 adipocytes were treated with FS and nonfermented soybean (NFS) extract during differentiation for 10 days in vitro. Oil red 0 staining was performed and glycerol-3-phosphate dehydrogenase (GPDH) activity was measured for analysis of fat accumulation. Expressions of adipogenic genes were measured.
RESULTS: Soluble extract of soybean fermented with Aspergillus oryzae GB107 contained higher levels of low-molecular-weight protein than conventional soybean protein did. FS extract (50 pg/ml) inhibited adipocyte differentiation and fat accumulation during differentiation of 3T3-L1 preadipocytes for 10 days in vitro. Significantly lower GPDH activity was observed in differentiated adipocytes treated with the FS extract than those treated with NFS extract. Treatment with FS extract resulted in decreased expression levels of leptin, adiponectin, and adipogenin genes, which are associated with adipogenesis.
CONCLUSIONS: This report is the first to demonstrate that the water-soluble extract from FS inhibits fat accumulation and lipid storage in 3T3-L1 adipocytes. Thus, the soybean extract fermented with A. oryzae GB107 could be used to control lipid accumulation in adipocytes. - The Regulation of Oxytocin Receptor Gene Expression during Adipogenesis
K. J. Yi, K. H. So, Y. Hata, Y. Suzuki, D. Kato, K. Watanabe, H. Aso, Y. Kasahara, K. Nishimori, C. Chen, K. Katoh, S. G. Roh
JOURNAL OF NEUROENDOCRINOLOGY, 27, 5, 335, 342, WILEY-BLACKWELL, 2015年05月
英語, 研究論文（学術雑誌）, Although it has been reported that oxytocin stimulates lipolysis in adipocytes, changes in the expression of oxytocin receptor (OTR) mRNA in adipogenesis are still unknown. The present study aimed to investigate the expression of OTR mRNA during adipocyte differentiation and fat accumulation in adipocytes. OTR mRNA was highly expressed in adipocytes prepared from mouse adipose tissues compared to stromal-vascular cells. OTR mRNA expression was increased during the adipocyte differentiation of 3T3-L1 cells. OTR expression levels were higher in subcutaneous and epididymal adipose tissues of 14-week-old male mice compared to 7-week-old male mice. Levels of OTR mRNA expression were higher in adipose tissues at four different sites of mice fed a high-fat diet than in those of mice fed a normal diet. The OTR expression level was also increased by refeeding for 4h after fasting for 16h. Oxytocin significantly induced lipolysis in 3T3-L1 adipocytes. In conclusion, a new regulatory mechanism is demonstrated for oxytocin to control the differentiation and fat accumulation in adipocytes via activation of OTR as a part of the hypothalamic-pituitary-adipose axis. - Neurotensin enhances sperm capacitation and acrosome reaction in mice
Yuuki Hiradate, Hiroki Inoue, Norio Kobayashi, Yoshiki Shirakata, Yutaka Suzuki, Aina Gotoh, Sang-Gun Roh, Takafumi Uchida, Kazuo Katoh, Manabu Yoshida, Eimei Sato, Kentaro Tanemura
Biology of Reproduction, 91, 2, Society for the Study of Reproduction, 2014年08月01日
英語, 研究論文（学術雑誌）, Neurotensin (NT) has multiple functions, ranging from acting as a neurotransmitter to regulating intestinal movement. However, its function in reproductive physiology is unknown. Here, we confirmed the expression and localization of NT receptors (NTR1) in mouse epididymal spermatozoa and investigated the effect of NT on sperm function. Sperm protein tyrosine phosphorylation, one of the indices of sperm capacitation, was facilitated dose-dependently by NT administration. In addition, the acrosome reaction was promoted in capacitated spermatozoa, and addition of a selective antagonist of NTR1 and NTR2 blocked the induction. Furthermore, intracellular calcium mobilization by NT addition was observed. This showed that NT was an accelerator of sperm function via its functional receptors. The presence of NT was confirmed by immunohistochemistry and its localization was observed in epithelia of the uterus and oviduct isthmus and ampulla, which correspond to the fertilization route of spermatozoa. The NT mRNA level in ovulated cumulus cell was remarkably increased by treatment with human chorionic gonadotropin (hCG). Using an in vitro maturation model, we analyzed the effects of FSH, epidermal growth factor (EGF), estradiol, and progesterone in NT production in cumulus cells. We found that FSH and EGF upregulated NT release and mRNA expression. Both FSH- and EGF-induced upregulation were inhibited by U0126, an MAPK kinase inhibitor, indicating that FSH and EGF regulate NT expression via a MAPK-dependent pathway. This evidence suggests that NT can act as a promoter of sperm capacitation and the acrosome reaction in the female reproductive tract. - 反芻動物の糖脂質代謝系におけるChemerinの生理的作用機構と生産性との関連
鈴木裕, 北山峻, 山内恵利, SONG Sanghoun, 加藤和雄, ROH Sanggun
栄養生理研究会報, 57, 1, 45, 54, 家畜栄養生理研究会, 2013年05月, ［筆頭著者］
日本語 - The Regulation of Chemerin and CMKLR1 Genes Expression by TNF-α, Adiponectin, and Chemerin Analog in Bovine Differentiated Adipocytes.
Suzuki Y, Hong YH, Song SH, Ardiyanti A, Kato D, So KH, Katoh K, Roh SG
Asian-Australasian journal of animal sciences, 25, 9, 1316, 1321, ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2012年09月, ［筆頭著者］
英語, 研究論文（学術雑誌）, Adipokines, adipocyte-derived protein, have important roles in various kinds of physiology including energy homeostasis. Chemerin, one of adipocyte-derived adipokines, is highly expressed in differentiated adipocytes and is known to induce macrophage chemotaxis and glucose intolerance. The objective of the present study was to investigate the changes of chemerin and the chemokine-like-receptor 1 (CMKLR1) gene expression levels during differentiation of the bovine adipocyte and in differentiated adipocytes treated with tumor necrosis factor-alpha (TNF-alpha), adiponectin, leptin, and chemerin (peptide analog). The expression levels of the chemerin gene increased at d 6 and 12 of the differentiation period accompanied by increased cytoplasm lipid droplets. From d 6 onward, peroxisome proliferator-activated receptor-gamma 2 (PPAR-gamma 2) gene expression levels were significantly higher than that of d 0 and 3. In contrast, CMKLR1 expression levels decreased at the end of the differentiation period. In fully differentiated adipocytes (i.e. at d 12), the treatment of TNF-alpha and adiponectin upregulated both chemerin and CMKLR1 gene expression levels, although leptin did not show such effects. Moreover, chemerin analog treatment was shown to upregulate chemerin gene expression levels regardless of doses. These results suggest that the expression of chemerin in bovine adipocyte might be regulated by chemerin itself and other adipokines, which indicates its possible role in modulating the adipokine secretions in adipose tissues. - Chemerin analog regulates energy metabolism in sheep
Yutaka Suzuki, Sang-Houn Song, Katsuyoshi Sato, Kyoung-Ha So, Astrid Ardiyanti, Shun Kitayama, Yeon-Hee Hong, Sung-Dae Lee, Ki-Choon Choi, Akihiko Hagino, Kazuo Katoh, Sang-Gun Roh
ANIMAL SCIENCE JOURNAL, 83, 3, 263, 267, WILEY-BLACKWELL, 2012年, ［筆頭著者］
英語, 研究論文（学術雑誌）, Accumulating data suggest a relationship between chemerin and energy metabolism. Our group previously described gene cloning, expression analysis and the regulatory mechanism of chemerin and its own receptor in mice and cattle. The objective of the present study was to investigate the physiological effect of chemerin on endocrine changes and energy metabolism in sheep using a biologically stable chemerin analog. The chemerin analog was intravenously administrated (100 or 500 mu g/head) to sheep, and plasma insulin and metabolites (glucose, nonesterified fatty acids (NEFA), triglyceride, total cholesterol and high-density lipoprotein (HDL) cholesterol) were analyzed. The chemerin analog dramatically increased the insulin levels, and glucose levels were decreased. NEFA levels were slightly decreased at 20 min but then increased gradually from 60 to 180 min after analog administration. In addition, injection of the chemerin analog immediately increased triglyceride and total cholesterol but not HDL levels. These results suggested that chemerin analog regulated insulin secretion related to glucose metabolism and the release of triglycerides in sheep in vivo. This study provides new information about endocrine and metabolic changes in response to chemerin in sheep.
その他活動・業績
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鈴木裕, 林英明, 芳賀聡, 小池聡, 小林泰男, 日本畜産学会第129回大会要旨集, 130, 131, 2021年09月 - 他菌の発酵産物がルーメン細菌Selenomonas ruminantium の運動性と増殖性に及ぼす影響
宮崎黎, 鈴木裕, 小林泰男, 小池聡, 日本畜産学会第128回大会要旨集, 2021年03月 - Effect of early roughage feeding on growth and hindgut environment of pre-weaned calves
Heshan Kodithuwakku, 大和田尚, 平野和夫, 三浦広卓, 鈴木裕, 小林泰男, 小池聡, 本畜産学会第128回大会要旨集, 1, 3, 2021年03月 - 子牛における消化管の発達：草食動物としての機能獲得とそのメカニズム
鈴木裕, 連携型博士研究人材総合育成システム 次世代研究者シンポジウム 2020, 2020年10月 - Distribution of core rumen microbes and its relationship to feed efficiency in Japanese Black cattle
Miura, H, Takeda, M, Akiyama, Y, Lahrita, L, Suzuki, Y, Kobayashi, Y, Koike, S, The 12th China-Japan-Korea Joint Symposium on Rumen Metabolism and Physiology, 2019年10月 - Effect of cashew nut shell liquid feeding on intestinal environment of broiler chickens
Hosokawa, M, Hikita, C, Suzuki, Y, Koike, S, Kobayashi, Y, The 12th China-Japan-Korea Joint Symposium on Rumen Metabolism and Physiology, 2019年10月 - The rumen basal layer cells express esophageal undifferentiated cell markers
Mizutani, R, Koike, S, Kobayashi, Y, Suzuki, Y, The 12th China-Japan-Korea Joint Symposium on Rumen Metabolism and Physiology, 2019年10月 - Upregulation of IgA secretion-related genes in salivary gland of growing calves
Takahashi, H, Koike, S, Kobayashi, Y, Suzuki, Y, The 12th China-Japan-Korea Joint Symposium on Rumen Metabolism and Physiology, 2019年10月 - Chemerin is produced in intestinal mucosa with a potential role in epithelial barrier function in calves.
Suzuki, Y, Hokkaido University and Zhejiang University Joint Symposium 2019, 2019年09月 - Exploration of undifferentiated cells in bovine rumen epithelial tissue
Suzuki, Y., Mizutani, R., Koike, S., Kobayashi, Y., Advances in Animal Biosciences (Proceedings of the XIIIth International Symposium on Ruminant Physiology ISRP 2019), 2019年09月 - Development of salivary IgA secretory ability in weaned calves
Hiroe Takahashi, Yutaka Suzuki, Satoshi Haga, Satoshi Koike, Yasuo Kobayashi, Advances in Animal Biosciences (Proceedings of the XIIIth International Symposium on Ruminant Physiology ISRP 2019), 10, 3, 518 (P45), 2019年09月, ［査読有り］
英語, 研究発表ペーパー・要旨（国際会議） - 子牛における唾液IgA分泌機能の発達
高橋宏枝, 鈴木裕, 芳賀聡, 小池聡, 小林泰男, 北海道畜産草地学会第8回大会要旨集, 2019年08月 - 抗菌性タンパク質Chemerinが仔牛腸内細菌の活性に及ぼす影響
久保田奏子, 鈴木裕, 小池聡, 小林泰男, 第52回 ルーメン研究会要旨集, 2018年11月 - カシューナッツ殻液製剤給与がブロイラー鶏の消化管環境に及ぼす影響
細川美律, 疋田千枝, 鈴木裕, 小池聡, 小林泰男, 北海道畜産草地学会第7回大会要旨集, 2018年09月 - 仔ウシ小腸における抗菌性タンパク質Chemerinの腸内細菌叢への作用
久保田奏子, 小池聡, 小林泰男, 鈴木裕, 北海道畜産草地学会第7回大会要旨集, 2018年09月 - ウシ口腔内の反芻残渣を用いた簡易ルーメン菌叢モニタリング手法の検討
三浦広卓, 小池聡, 新居彦治, 大谷喜永, 鈴木裕, 小林泰男, 北海道畜産草地学会第7回大会要旨集, 2018年09月 - Enhancement of ruminal acetate production by supplementing cellulose acetate, a new feed additive candidate
Watabe, Y, Suzuki, Y, Koike, S, Shimamoto, S, Kobayashi, Y, 10th International Symposium on Nutrition of Herbivores, 2018年09月 - The effect of chemerin as a host-derived factor on intestinal microbial activity in calves
Yutaka Suzuki, Kanako Kubota, Hiroto Miura, Satoshi Haga, Sanggun Roh, Satoshi Koike, Yasuo Kobayashi, 2018 ASAS-CSAS Annual Meeting ＆ Trade Show, 135, 2018年07月
英語, 研究発表ペーパー・要旨（国際会議） - Evaluation of trehalose as a functional material to improve gut health in preweaned calves
Miura, H, Koike, S, Mukai, K, Suzuki, Y, Kobayashi, Y, Rowett-INRA 2018 Gut Microbiology, 2018年06月 - 黒毛和種ルーメン由来新規デンプン分解菌の増殖因子の探索
中光大輔, 小池聡, 鈴木裕, 小林泰男, 日本畜産学会第124回大会要旨集, 125, 2018年03月 - ウシ第１胃（ルーメン）における生体防御とケモカインの関係
西原昂来, 鈴木裕, 金多慧, 芳賀聡, 盧尚建, 2017年度生命科学系学会合同年次大会（ConBio2017）要旨集, 2P-1287, 2017年12月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - Influence of trehalose feeding on fecal microbiota in preweaned calves
Miura, H, Koike, S, Mukai, K, Suzuki, Y, Kobayashi, Y, 11th Joint Symposium on Rumen Metabolism and Physiology, 2017年10月 - Utilization of saccharides by non-fibrolytic ruminal bacteria constituting a fibrolytic consortium
Murazumi, Y, Taketa, H, Fukuma, N, Koike, S, Inamura, Y, Yamano, M, Aizawa, T, Suzuki, Y, Kobayashi, Y, 11th Joint Symposium on Rumen Metabolism and Physiology, 2017年10月 - Mitigation of methane emission from feces an slurry of Holstein cattle by applying ginkgo fruit: in vitro study
Shintani, R, Oh, S, Suzuki, Y, Koike, S, Kobayashi, Y, 11th Joint Symposium on Rumen Metabolism and Physiology, 2017年10月 - Use of cellulose acetate as a feed additive candidate for ruminants
Watabe, Y, Imagawa, T, Koike, S, Suzuki, Y, Shimamoto, S, Kobayashi, Y, 11th Joint Symposium on Rumen Metabolism and Physiology, 2017年10月 - Rumen epithelial cells as a source of inflammatory cytokines
K. Nishihara, Y. Suzuki, S. Haga, D.H. Kim, S.G. Roh, The 11th Joint Symposium of Japan-China-Korea on Rumen Metabolism and Physiology, 50, 2017年10月
英語 - 水牛ルーメン由来繊維分解性細菌Ruminococcus flavefaciens OS14株の機能および生態
金城円花, Phoonpong Boonsaen, Suriya Sawanon, 鈴木裕, 小池聡, 小林泰男, 北海道草地畜産学会第6回大会要旨集, 2017年09月 - Potency of ginkgo fruit for modulation of rumen microbiota and fermentation
Oh, S, Suzuki Y, Koike, S, Kobayashi, Y, 平成29年度栄養生理研究会秋季集談会, 2017年09月 - Bean husk, an agricultural by-product having functionality in animal nutrition and health
Myint, H, Kishi, H, Iwahashi, Y, Suzuki, Y, Koike, S, Kobayashi, Y, 平成29年度栄養生理研究会秋季集談会, 61, 2, 13, 19, 2017年09月
家畜栄養生理研究会, 英語 - 沼沢水牛ルーメンから分離した繊維分解菌Ruminococcus flavefaciensの系統、生態および機能
小林泰男, Phoompong Boonsaen, 金城円花, Suriya Sawanon, 日本畜産学会第123回大会要旨集, 2017年09月 - 黒毛和種牛から分離したデンプン分解性ルーメン細菌の特性評価
小池聡, 秋山瑤子, 鈴木裕, 小林泰男, 日本畜産学会第123回大会要旨集, 2017年09月 - Isolation and partial characterization of starch degrading bacteria belonging to core phylotypes of rumen microbiota in Japanese Black cattle
Koike S, Akiyama Y, Hashimoto T, Inoue R, Endo T, Suzuki Y, Kobayashi Y, Congress on Gastrointestinal Function, 2017年04月 - Chemerin is present in intestinal epithelia of calves with a potential role in barrier function
Suzuki Y, Roh S, Hayashi H, Koike S, Kobayashi Y, Congress on Gastrointestinal Function 2017, 2017年04月 - トレハロース給与が哺乳子牛の腸内環境に与える影響
三浦広卓, 小池聡, 鈴木裕, 向井和久, 小林泰男, 日本畜産学会第122回大会要旨集, 2017年03月 - ウシルーメン絨毛組織の発達におけるIGFとIGFBPsの役割
西原昂来, 鈴木裕, 金多慧, 中野美智, 中野美和, 石崎宏, 芳賀聡, 盧尚建, 日本畜産学会第122回大会講演要旨, 109, 2017年03月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - Gene expression and secretion of chemerin in bovine mammary epithelial cells.
Suzuki Yutaka, Chiba Sachi, Haga Satoshi, Roh Sanggun, 2016 Joint Annual Meeting (JAM) ASAS-ADSA-CSAS-WSASAS in Salt Lake City Program book, 144, 2016年07月, ［査読有り］
英語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - 離乳前後の仔牛の肝臓組織における網羅的トランスクリプトーム解析
盧尚建, 近藤光彦, 鈴木裕, 加藤大地, 芳賀聡, 川原玲香, 河野友宏, 鈴木啓一, 加藤和雄, 日本畜産学会第121回大会講演要旨, 198, 2016年03月
日本語 - 黒毛和種牛のルーメン発達関連因子の解析
加藤大地, 鈴木裕, 芳賀聡, 蘇敬夏, 川原玲香, 河野友宏, 加藤和雄, 盧尚建, 日本畜産学会第121回大会講演要旨, 243, 2016年03月
日本語 - 反芻動物の生産に関する最近の研究動向(4)
芳賀 聡, 鈴木 裕, 加藤 大地, 畜産の研究 = Sustainable livestock production and human welfare, 69, 12, 1005, 1009, 2015年12月
養賢堂, 日本語 - 反芻動物におけるヘパトカインとしてのChemerinとANGPTL8 の発現調節
鈴木裕, 中野美智, 芳賀聡, 中島恵一, 加藤和雄, 盧尚建, 平成27年度 家畜栄養生理研究会, 2015年11月 - 周産期の肝臓組織におけるANGPTL8の発現調節
中野美智, 鈴木裕, 芳賀聡, 中野美和, 宮地慎, 石崎宏, 蘇敬夏, 加藤和雄, 盧尚建, 日本畜産学会第120回大会講演要旨, 90, 2015年09月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - 泌乳期と乾乳期の乳牛における内分泌因子ケメリンのシグナル調節とその役割
鈴木裕, 中島恵一, 芳賀聡, 中野美和, 宮地慎, 石崎宏, 盧尚建, 日本畜産学会第120回大会講演要旨, 120th, 42, 2015年09月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - Expressional regulation of chemerin and its receptors in the liver and adipose tissues of young cattle by weaning and nutrition
Yutaka Suzuki, Daichi Kato, Mitsuhiko Kondo, Hizuru Hatanaka, Satoshi Haga, Takafumi Gotoh, Sanggun Roh, ADSA-ASAS JAM 2015, 588, 2015年07月, ［査読有り］
英語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - RNA-Seqによる黒毛和種牛ルーメン発達関連遺伝子の網羅的トランスクリプトーム解析
加藤大地, 鈴木裕, 芳賀聡, 蘇敬夏, 川原玲香, 河野友宏, 加藤和雄, 盧尚健, 日本畜産学会第119回大会講演要旨, 186, 2015年03月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - ウシBetatrophin の遺伝子発現調節について
盧尚建, 山内恵利, 鈴木裕, 加藤大地, 近藤光彦, 畑中陽弦, 芳賀聡, 蘇敬夏, SeungJu Park, Myunggi Baik, 加藤和雄, 日本畜産学会第119回大会講演要旨, 184, 2015年03月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - ウシ乳腺上皮細胞におけるケメリンの糖・脂質代謝関連遺伝子発現の調節作用
加藤大幾, 鈴木裕, 芳賀聡, 蘇敬夏・Lee Hong-Gu・Jung U-Suk, 加藤和雄, 盧尚建, 日本畜産学会第119回大会講演要旨, 184, 2015年03月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - ウシ培養肝細胞におけるケメリン調節因子に関する研究
近藤光彦, 鈴木裕, 芳賀聡, 畑中陽弦, 加藤大地, 蘇敬夏, 加藤和雄, 盧尚建, 日本畜産学会第119回大会講演要旨, 184, 2015年03月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - Digital Differential Displayによるウシルーメン発達関連遺伝子のIn silico同定
加藤大地, 鈴木裕, 芳賀聡, 蘇敬夏, 中野美和, 石崎宏, 加藤和雄, 盧尚建, 第20回 Hindgut Club Japan, 2014年12月 - 絶食・再給餌によるマウス肝臓および脂肪組織におけるケメリンの発現調節
鈴木裕, 蘇敬夏, 畑優紀, 熊谷優希, 加藤和雄, 盧尚建, 第48回日本栄養・食糧学会東北支部大会要旨集, Ⅳ-10, 2014年11月 - Chemerin mRNA expression is regulated by insulin and fatty acids in the Liver of calves
鈴木裕, 北山俊, 近藤光彦, 芳賀聡, 加藤和雄, 盧尚健, Joint ISNH/ISRP International conference 2014, 30, 272, 2014年09月, ［査読有り］
英語, 研究発表ペーパー・要旨（国際会議） - Gene expression and regulation of betatrophin in cultured hepatocytes of preweaning and postweaning calves
盧尚建, 山内恵利, 鈴木裕, 北山俊, 近藤光彦, 中野美和, 石崎宏, 芳賀聡, 加藤和雄, Joint ISNH/ISRP International conference 2014, 30, 306, 2014年09月, ［査読有り］
英語, 研究発表ペーパー・要旨（国際会議） - ウシBetatropinの発現と調節
山内恵利, 鈴木裕, 東北, 蘇敬夏, 東北, 農, 芳賀聡, 加藤和雄, 盧尚建, 東北大院農, 日本畜産学会第118回大会講演要旨, 231, 2014年03月
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議） - Deficiency of adipogenin decreases fat accumulation and alters glucose metabolism
Abstract book of 4th International Congress on Abdominal Obesity, 111, 2013年09月 - Adipogenin, a regulatory gene plays role in fat accumulation of adipogenesis
So, KH, Song, SH, Hata, Y, Kumagai, Y, Suzuki, Y, Katoh, K, Roh, SG, Abstract book of 4th International Congress on Abdominal Obesity, 110, 2013年09月 - Effect of propionate, palmitate and insulin on chemerin gene expression in monolayer cultures of bovine hepatocytes.
盧尚建, 北山峻, 鈴木裕, 蘇敬夏, 李權正, 山内恵利, 芳賀聡, 加藤和雄, ADSA-ASAS JAM2013, T340, 2013年07月, ［査読有り］
英語, 研究発表ペーパー・要旨（国際会議） - 反芻動物の糖脂質代謝系におけるChemerinの生理的作用機構と生産性との関連
鈴木裕, 北山峻, 山内恵利, 宋相憲, 加藤和雄, 盧尚建, 平成25年度 家畜栄養生理研究会 春季集談会, 2013年05月 - The changes of chemerin and chemerin receptor to regulate lipid metabolism in liver and pituitary gland
Sanggun Roh, Shun Kitayama, Astrid Ardiyanti, Yutaka Suzuki, Eri Yamauchi, Daiki Kato, Kwonjung Yi, Kyoungha So, Akihiko Hagino, Kazuo Katoh, FASEB JOURNAL, 27, 2013年04月
FEDERATION AMER SOC EXP BIOL, 英語, 研究発表ペーパー・要旨（国際会議） - アディポジェニン遺伝子欠損における脂肪代謝作用とその機能解析
蘇敬夏, 立崎泰正, Astrid Aridiyanti, 鈴木裕, 宋相憲, 盧尚健, 加藤和雄, Adiposcience研究会2012, 2012年08月 - The expression in and role of oxytocin receptor on adipose tissue lipid metabolism under different feeding conditions in mice
Roh, SG, So, KH, Suzuki, Y, Chen, C, Katoh, K, The Endocrine Society's 94th Annual Meeting and Expo, SAT-105, 2012年06月 - Deletion of adipogenin gene in mice is effected to adipogenesis and deferred to growth
So Kyoung-Ha, Taisei Tachizaki, Astrid Aridiyanti, Yutaka Suzuki, Sang-Houn Song, Sang-gun Roh, Kazuo Katoh, FASEB JOURNAL, 26, 2012年04月
FEDERATION AMER SOC EXP BIOL, 英語, 研究発表ペーパー・要旨（国際会議） - ウシの脂肪細胞と乳腺上皮細胞におけるオキシトシン受容体(OXT-R)の発現変動
盧尚建, 佐藤達彦, 大谷喜永, 蘇敬夏, 宋相憲, 鈴木裕, 萩野顕彦, 加藤和雄, 日本畜産学会第115回大会要旨集, 233, 2012年03月 - 反芻動物におけるケメリン（Chemerin）の発現調節機構と糖脂質代謝における生理的作用の解明
鈴木裕, 宋相憲, 佐藤勝祥, 菊池佑弥, 盧尚建, 加藤和雄, 日本畜産学会第114回大会要旨集, 73, 2011年08月 - 脂肪細胞分化形成に関わるAdipogeninの機能解析
宋相憲, 鈴木裕, 盧尚建, 加藤和雄, 家畜栄養生理研究会 平成23年度春季集談会, 2011年05月 - ヒト肝がん細胞（HepG2）におけるChemerin分泌調節
盧尚建, 宋相憲, 鈴木裕, Asrdiyanti Astrid, 加藤和雄, 分子生物学会2010, 1P-0029, 2010年12月 - 牛肉,豚肉および鶏肉の購入に対する消費者の意識調査
横田 祥子, 池田 直史, 鈴木 裕, 複合生態フィールド教育研究センター報告, 26, 1, 8, 2010年12月
東北大学大学院農学研究科附属複合生態フィールド教育研究センター, 日本語 - 筋芽細胞および脂肪細胞の脂肪蓄積能におけるAdipogeninの機能解析
宋相憲, 鈴木裕, 盧尚建, 加藤和雄, Adiposcience研究会2010, 36, 2010年08月 - 脂肪細胞と肝細胞におけるChemerinとChemerin受容体の発現動態
鈴木裕, 宋相憲, 盧尚建, 加藤和雄, Adiposcience研究会2010, 33, 2010年08月 - Possible functional roles of chemerin, a new adipokine, in domestic animals
Sang-Gun Roh, Sang-Houn Song, Yutaka Suzuki, Kazuo Katoh, FASEB JOURNAL, 24, 2010年04月
FEDERATION AMER SOC EXP BIOL, 英語, 研究発表ペーパー・要旨（国際会議） - ウシ脂肪細胞におけるケメリン（Chemerin）とその受容体の発現調節機構
鈴木裕, 宋相憲, 盧尚建, 加藤和雄, 日本畜産学会第112回大会要旨集, 98, 2010年03月 - Adipogeninの発現調節について
盧尚建, 宋相憲, 鈴木裕, 佐々木晋一, 加藤和雄, 日本畜産学会第112回大会要旨集, 110, 2010年03月
書籍等出版物
講演・口頭発表等
- ウシ反芻胃における幹細胞探索の試みと組織発達における役割
鈴木裕
日本畜産学会第129回大会 パラレルシンポジウムII 畜産学における組織幹細胞研究の現在・未来, 2021年09月15日
［招待講演］ - Introduction of our group & research: Cellular mechanism of rumen development process
Suzuki, Y
Seminar talk in Can Tho University, 2019年04月14日
［招待講演］ - A study on the developmental mechanism of gastrointestinal tract in calves
Seminar talk in University of Alberta, 2018年09月26日
［招待講演］ - Study on chemerin as a new player in bovine epithelial barrier system
Suzuki, Y
Seminar talk in University of Saskatchewan, VIDO center, 2018年09月14日
［招待講演］
共同研究・競争的資金等の研究課題
- ルーメン細菌叢制御を目指した子ウシ唾液抗体の分泌機能の解明（Ⅲ）
研究助成
2024年04月 - 2025年03月
伊藤記念財団, 北海道大学, 研究代表者 - ルーメン細菌叢制御を目指した子ウシ唾液抗体の分泌機能の解明（Ⅱ）
研究助成
2023年04月 - 2024年03月
伊藤記念財団, 北海道大学, 研究代表者 - 健全な消化管発達の基盤となるウシ反芻胃の幹細胞ニッチの解明
科学研究費助成事業 基盤研究(C)
2021年04月 - 2024年03月
鈴木 裕
ウシの反芻胃（ルーメン）は離乳後の成長期に急速に発達するが、その細胞的・分子的な機序には不明な点が多い。当グループは予備研究において、ルーメン上皮では基底層のみに増殖活性を持つ細胞群が存在することを見出した。これらの細胞が組織幹細胞あるいは前駆細胞として振る舞うことで、ルーメン組織の発達に関与すると考え、その細胞の増殖や制御する組織内の微小環境（ニッチ）の存在を仮定して、その解明を目指した。
本年度はニッチを構成する分子の探索を目指した。まず、ルーメンで発現するニッチ因子を同定するために、レーザーマイクロダイセクションによりルーメン上皮細胞と固有層を分取する方法の確立を目指したが、薄切組織中RNAの保存性が十分ではなかったため、改善作業を継続している。代替策として、凍結した組織全体からRNAを抽出し、ニッチ構成分子として予想される細胞外マトリックスと成長因子の発現解析を行った。様々な上皮組織において細胞増殖制御に関与することが知られるラミニンおよびEGF、FGF、TGF-bファミリーの発現が確認された。相関分析から、これらの中の一部の因子はルーメン上皮細胞の増殖活性（マーカー遺伝子発現量）と強い相関を持つことが明らかになった。
本年度の研究結果から、ルーメン上皮細胞を取り囲むニッチ因子としてラミニンや各種の成長因子が示唆された。今後は網羅的解析により他のニッチ候補分子を探索するとともに、培養ルーメン上皮細胞などを用いてニッチ候補分子の直接的な生理的な役割を明らかにすることを目指す。
日本学術振興会, 基盤研究(C), 北海道大学, 研究代表者, 21K05900 - ルーメン細菌叢制御を目指した子ウシ唾液抗体の分泌機能の解明
研究助成
2022年04月 - 2023年03月
鈴木 裕
伊藤記念財団 - 非侵襲的な免疫機能マーカーとしての子牛の唾液・糞便中免疫グロブリンG濃度の検討
研究助成
2021年09月 - 2022年09月
鈴木裕
栗林育英学術財団, 研究代表者 - 機能性アルキルフェノール含有植物の多面的活用による反すう家畜由来メタンガスの削減
科学研究費助成事業 基盤研究(B)
2018年04月 - 2022年03月
小林 泰男, 鈴木 裕, 小池 聡
東南アジアは世界で有数の反芻家畜からのメタン排出が多大な地域であり、カシュー殻液に代表されるメタン削減素材の機能評価が望まれているため、試験を実施した。すなわち、沼沢水牛4頭を用いたカシューナッツ殻液製剤給与試験を実施した。沼沢水牛はタイのみならず、東南アジアの代表的家畜で飼養年数も長いことから、ルーメンおよび糞便からのメタン削減のより広範囲にわたる高いインパクトが期待できる。
カシューナッツ殻液製剤給与により、沼沢水牛ルーメン液からのメタン生成ポテンシャルは、無添加飼料給与時（対照）に比べ73％低下した。一方、プロピオン酸のモル比は31％増加した。これらはルーメン内での代謝性水素の処理系がメタン生成からフマル酸還元にシフトした結果、ひきおこされたものと推察できた。微生物相の解析結果はこの推察を明瞭に裏付けていた。すなわち、カシューナッツ殻液製剤給与により、水素やギ酸を生成する菌群（TreponemaやRuminococcus）が検出率を下げる一方、コハク酸（プロピオン酸前駆体）やプロピオン酸の生成に関与する菌群（PrevotellaおよびSucciniclasticum）が検出率を上げた。さらに新しい知見として、メタン生成古細菌の構成も変化し、Methanomassiliicoccaceaeが減少、Methanomicrococcusが増加した。
一方、糞便でもメタン生成ポテンシャルの低下（74％）が認められた。その際、酢酸比の低下とプロピオン酸比の増加が生じた。メタン減少は、微生物相の変化に対応したものと推測された。すなわち、Unclassified RuminococcaceaeおよびMethanomassiliicoccaceaeの減少が認められた。
以上より、カシューナッツ殻液製剤給与によるルーメンおよび糞便からのメタン減少は、沼沢水牛ではその他の牛よりもやや明確に生じること、それは微生物相の変化と連動していることが分かった。
日本学術振興会, 基盤研究(B), 北海道大学, 研究分担者, 18H02322 - 組織幹細胞から見たウシ反芻胃の発達メカニズムの解明
科学研究費助成事業 若手研究
2019年04月 - 2021年03月
鈴木 裕
ウシの栄養獲得の要であるルーメン（反芻胃）は離乳前後の時期から急速に発達するが、どのような細胞群が組織発達に関わるのかといったミクロなメカニズムは明らかではなかった。動物組織の発達や維持においては、組織幹細胞による分化細胞の供給が重要な役割を担っていることから、本研究ではルーメン上皮組織における組織幹細胞の探索と組織発達への関与を検討した。結果として、ルーメン上皮基底層に幹細胞マーカーおよび細胞増殖マーカーを発現する細胞群を見出した。また、細胞増殖活性は離乳前の時期に高いことが明らかになった。従って、この時期に始まる組織幹細胞の増殖活性化により組織発達が引き起こされることが示唆された。
日本学術振興会, 若手研究, 北海道大学, 研究代表者, 19K15958 - 仔牛における反芻胃を中心とした消化管機能発達メカニズムの解明 ～次世代ルミノロジーの開拓を目指して～
科学技術人材育成のコンソーシアムの構築事業：連携型博士研究人材総合育成システムの構築
2017年04月 - 2021年03月
鈴木裕
文部科学省・JST, 研究代表者 - 新規抗菌性タンパク質による子牛の腸管バリア機能への寄与の解明
研究助成金
2019年06月 - 2020年06月
鈴木裕
旗影会, 研究代表者 - 細菌由来分子に着目したルーメン上皮バリアの発達機序の解明
科学研究費助成事業 若手研究(B)
2017年04月 - 2019年03月
鈴木 裕
本研究は、ウシのルーメン上皮バリアの機能改善を念頭に、子牛の時期のルーメン上皮バリアの成立機序の解明を目指した。はじめに組織発達前後のルーメンにおいてRNA-seqを行った結果、菌体成分が上皮の免疫・防御反応を促進する可能性が示唆された。同時に、組織発達時に細胞供給源の探索を行った結果、ルーメン上皮基底部において未分化および細胞増殖マーカーを発現する細胞群の存在が示唆された。さらに、ルーメン上皮細胞の培養系を改良し、上皮シートを体外で培養することが可能になった。今後は、確立したルーメン細胞培養系を利用して、上皮バリアと免疫機構を誘導する分子的機序を詳細に検討する必要性が提示された。
日本学術振興会, 若手研究(B), 北海道大学, 研究代表者, 17K15358 - 仔牛の健全な消化機能の基盤となるルーメン幹細胞の同定
2017年09月 - 2018年09月
鈴木裕
栗林育英学術財団, 研究代表者 - 乳房炎防除を目的とした多機能性の新規分泌タンパク質による乳腺バリア調節機能の解明
研究助成（奨励）
2017年09月 - 2018年09月
鈴木裕
秋山記念生命科学振興財団, 研究代表者 - ルーメンアシドーシス防除を目指した新規ルーメン幹細胞培養モデルの開発
研究助成
2017年07月 - 2018年07月
鈴木裕
伊藤記念財団, 研究代表者 - 反芻家畜におけるアディポカインとしてのケメリンの内分泌代謝と生産性への影響
科学研究費助成事業 特別研究員奨励費
2014年04月 - 2016年03月
鈴木 裕
アディポカインであるケメリンは免疫系や糖代謝・脂質代謝を調節するという特徴を持つ。当研究室ではウシケメリンの一塩基多型が枝肉の脂肪交雑や脂肪酸組成に影響することを報告しているが、ウシにおけるケメリンシグナリングの調節機構や生理的作用は未解明である。本研究では、乳牛や肉牛の生産への応用を念頭に、以下１～３のテーマを設定し、ウシにおけるケメリンを中心とした代謝調節機構の解明を目指した。
１、ケメリン遺伝子発現の調節機構の解明：黒毛和種牛においてケメリンは脂肪組織に加え肝臓においても高発現していた。特に、肝臓のケメリン蛋白質は動物の離乳後に減少していた。一方で、育成期のウシでは、粗飼料と比較して濃厚飼料を給餌した際にケメリン発現量は上昇していた。また、周産期の乳牛において肝臓におけるケメリンの発現は分娩直後に低下していた。
２、ウシ血中ケメリン濃度変化の検討：タンパク質発現の変化にもかかわらず、離乳期におけるケメリンの血中濃度には変化が見られなかった。一方で、乳牛ではケメリンの血液濃度変化は肝臓における遺伝子発現変化と類似した傾向を示した。
３、ウシにおけるケメリンの生理的作用の解明：上述のようにケメリン発現は離乳により変化したが、この時肝臓のプロピオン酸を基質とする糖新生系酵素の発現にも大きな変動が見られた。以前の報告から、ケメリンは糖新生を阻害する作用が示唆されるが、本研究によりケメリンは離乳に伴う反芻動物の糖新生系の発達に重要な役割を持つことが示唆された。また、ケメリンは乳牛の乳房内における発現・分泌が確認され、乳腺上皮細胞の牛乳合成の促進作用や免疫細胞の誘引作用を持つことが示唆された。
以上の結果から、ウシにおいてケメリンは離乳やエネルギー摂取、妊娠・出産によりその発現量や分泌量が制御され、肝臓の糖代謝や乳腺の機能を制御することが示唆された。
日本学術振興会, 特別研究員奨励費, 東北大学, 研究代表者, 14J05480
