2024年09月, 第10回北海道大学部局横断シンポジウム研究助成採択 銀賞　　　　　　　　　　　　　　　

2024年08月, Hokkaido University, 2024 年度 One Health Research 推進費　　　　　　　　　　　　　　　
Investigating the dysregulated lipid metabolism in immune cells associated with severe influenza infection in LOX-1 knockout mice

2024年07月, FY2024 Next Generation Researcher Collaborative Research Support Program, Discovery of sphingomyelin synthase inhibitor from small-leaf nettle to control fatty liver disease　　　　　　　　　　　　　　　
FY2024 Next Generation Researcher Collaborative Research Support Program, Discovery of sphingomyelin synthase inhibitor from small-leaf nettle to control fatty liver disease

2022年08月, Hokkaido University, 2022年度 次世代研究者共同研究支援制度　　　　　　　　　　　　　　　
北海道の在来魚シシャモの肥満抑制効果に関する研究
Siddabasave gowda;Bomme gowda

2022年07月, Hokkaido University, 2022年度SOUSEI若手研究者支援プログラム助成金　　　　　　　　　　　　　　　
脂質代謝に関連する肥満症を食品バイオアクチブで克服する
Siddabasa gowda;Bomme gowda

2022年03月, 2020-2021 Top cited author from the Rapid Communications in Mass spectrometry　　　　　　　　　　　　　　　

2021年11月, Hokkaido University, 北海道大学-部局横断型シンポジウム / 助成金-銅賞　　　　　　　　　　　　　　　
インフルエンザ感染における新規腸管バイオマーカーの役割の検討
Siddabasave gowda;Bomme Gowda

2021年06月, Hokkaido University, 2021年度 次世代研究者共同研究支援制度　　　　　　　　　　　　　　　
"スフィンゴ脂質代謝に関連する昆布と肥満の関連性を解明"
Siddabasave gowda;Bomme Gowda

2021年04月, 日本学術振興会, 若手研究　　　　　　　　　　　　　　　
肥満に伴うスフィンゴ糖脂質の代謝におけるヒジキの機能的役割を探る
Siddabasavegowda;Bomme gowda

2020年06月, 北海道大学, スタートアップ助成金　　　　　　　　　　　　　　　

2017年12月, TBRF, Japan, Postdoctoral fellowship　　　　　　　　　　　　　　　
TBRF Postdoctoral Fellow (declined)
ボメゴウダシッダバサヴゴウダ

2016年02月, JASSO, Japan, Scholarship　　　　　　　　　　　　　　　
PhD scholar
ボメゴウダシッダバサヴゴウダ

2015年07月, ONO Pharmacetical, Travel Award　　　　　　　　　　　　　　　
14th Bioactive lipid conference, Hungary
ボメゴウダシッダバサヴゴウダ

2015年03月, Hokkaido University, Travel Award　　　　　　　　　　　　　　　
249th ACS meeting, Colorado, USA
ボメゴウダシッダバサヴゴウダ

2012年10月, Japanese governament, IGP-MEXT Scholarship　　　　　　　　　　　　　　　
Phd Research fellow
ボメゴウダシッダバサヴゴウダ

Discovering Novel Short‐ and Medium‐Chain Esters of Hydroxy Fatty Acids in Human Fecal Samples Using Untargeted Liquid Chromatography/Mass Spectrometry
Jayashankar Jayaprakash, Divyavani Gowda, Rachana M. Gangadhara, Shalini Jain, Hariom Yadav, Siddabasave Gowda B. Gowda, Shu‐Ping Hui
Rapid Communications in Mass Spectrometry, 39, 12, e10032, 2025年06月30日, ［国際誌］
英語, 研究論文（学術雑誌）, RATIONALE: Exploring novel metabolites produced by host gut microbiome communication is crucial for understanding their roles in various disease pathologies. We previously uncovered a novel class of lipids, short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), in mouse fecal samples and demonstrated their promising physiological functions in mammals. However, the discovery of SFAHFAs in human samples remains unexplored. METHODS: This study aimed to analyze the SFAHFAs and their structural analogs in human fecal samples using liquid chromatography/mass spectrometry. RESULTS: We identified 26 isomeric lipid species, including SFAHFAs and novel medium-chain fatty acid esters of hydroxy fatty acids (MFAHFAs). The detected SFAHFAs and MFAHFAs were characterized by accurate mass measurements using MSn analysis. The results were validated by matching the mass spectral fragmentation and retention time with authentic standards. Two new MFAHFAs, enanthic acid and caprylic acid esters of long-chain hydroxy fatty acids (C24 and C26), were detected and characterized for the first time in human fecal samples. Among the 26 isomeric lipid species, SFAHFA 2:0/24:0 or 4:0/22:0 and SFAHFA 2:0/24:1 were most abundant among the saturated and unsaturated SFAHFAs, respectively. CONCLUSIONS: This study offers the first insights into detecting and characterizing novel gut microbial lipids in human fecal samples. Further investigations are essential to recognize the metabolism and function of these lipids in the human gut.

Lipidomic Profiling of Edible Japanese Sea Urchins by LC–MS
Sahana Amai, Kisara Yuki, Siddabasave Gowda B. Gowda, Divyavani Gowda, Shu-Ping Hui
Foods, 2025年06月26日
研究論文（学術雑誌）

Integration of lipidomics with targeted, single cell, and spatial transcriptomics defines an unresolved pro-inflammatory state in colon cancer
Ramani Soundararajan, Michelle M Maurin, Jetsen Rodriguez-Silva, Gunjan Upadhyay, Ashley J Alden, Siddabasave Gowda B Gowda, Michael J Schell, Mingli Yang, Noah Jhad Levine, Divyavani Gowda, Punith M Sundaraswamy, Shu-Ping Hui, Lance Pflieger, Heiman Wang, Jorge Marcet, Carolina Martinez, Robert David Bennett, Allen Chudzinski, Andreas Karachristos, Timothy M Nywening, Paul M Cavallaro, Matthew Linley Anderson, Robert J Coffey, Michael V Nebozhyn, Andrey Loboda, Domenico Coppola, Warren Jackson Pledger, Ganesh V Halade, Timothy J Yeatman
Gut, 74, 4, 586, 602, 2025年04月, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Over a century ago, Virchow proposed that cancer represents a chronically inflamed, poorly healing wound. Normal wound healing is represented by a transitory phase of inflammation, followed by a pro-resolution phase, with prostaglandin (PGE2/PGD2)-induced 'lipid class switching' producing inflammation-quenching lipoxins (LXA4, LXB4). OBJECTIVE: We explored if lipid dysregulation in colorectal cancers (CRCs) is driven by a failure to resolve inflammation. DESIGN: We performed liquid chromatography and tandem mass spectrometry (LC-MS/MS) untargeted analysis of 40 human CRC and normal paired samples and targeted, quantitative analysis of 81 human CRC and normal paired samples. We integrated analysis of lipidomics, quantitative reverse transcription-PCR, large scale gene expression, and spatial transcriptomics with public scRNASEQ data to characterize pattern, expression and cellular localisation of genes that produce and modify lipid mediators. RESULTS: Targeted, quantitative LC-MS/MS demonstrated a marked imbalance of pro-inflammatory mediators, with a dearth of resolving lipid mediators. In tumours, we observed prominent over-expression of arachidonic acid derivatives, the genes encoding their synthetic enzymes and receptors, but poor expression of genes producing pro-resolving synthetic enzymes and resultant lipoxins (LXA4, LXB4) and associated receptors. These results indicate that CRC is the product of defective lipid class switching likely related to inadequate or ineffective levels of PGE2/PGD2. CONCLUSION: We show that the lipidomic profile of CRC tumours exhibits a distinct pro-inflammatory bias with a deficiency of endogenous resolving mediators secondary to defective lipid class switching. These observations pave the way for 'resolution medicine', a novel therapeutic approach for inducing or providing resolvins to mitigate the chronic inflammation driving cancer growth and progression.

Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study
Yifan Chen, Siddabasave Gowda B. Gowda, Divyavani Gowda, Jayashankar Jayaprakash, Lipsa Rani Nath, Atusko Ikeda, Yu Ait Bamai, Rahel Mesfin Ketema, Reiko Kishi, Hitoshi Chiba, Shu-Ping Hui
Diagnostics, 15, 6, 2025年03月16日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Background: Plasmalogens (Pls) are phospholipids with a unique structure, abundant in the brain and heart. Due to their chemical instability and analytical difficulties, less information is available compared to other phospholipids. The importance of Pls in several cellular processes is known, one of which is their protective effect against oxidative damage. The physiological role of Pls in human development has not been elucidated. Despite their clinical importance, the quantitative analysis of Pls in children's plasma has been limited. Methods: This study aims to determine the plasma levels of Pls in prepubertal children using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The plasma samples used were obtained from 9- to 12-year-old girls (n = 156) and boys (n = 178), n = 334 in total, who participated in the Hokkaido study. Results: Ethanolamine plasmalogen (PlsEtn) and choline plasmalogen (PlsCho), both carrying eicosapentaenoic acid, were significantly lower in girls than in boys. In both sexes, the plasmalogen levels for the 12-year-old children were lower than those for the 9-year-old children. PlsCho (16:0/18:2) was lower in the overweight children than in the normal-weight children for both sexes. PlsEtn (18:0/20:4) was the most abundant ethanolamine-type plasmalogen in both sexes. Conclusions: This study is the first report on plasmalogen levels and molecular types in children's plasma. This study provides the information needed to understand the role of Pls in human developmental processes and may open up new opportunities in the future to control age-related changes in Pls.

Determination of plasma lysophosphatidylethanolamines (lyso-PE) by LC-MS/MS revealed a possible relation between obesity and lyso-PE in Japanese preadolescent children: The Hokkaido study.
Nao Inoue, Siddabasave Gowda B Gowda, Divyavani Gowda, Toshihiro Sakurai, Atsuko Ikeda-Araki, Yu Ait Bamai, Rahel Mesfin Ketema, Reiko Kishi, Hitoshi Chiba, Shu-Ping Hui
Annals of clinical biochemistry, 62, 1, 34, 45, 2025年01月, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Lysophosphatidylethanolamines (lyso-PEs) are the partial hydrolysis products of phosphatidylethanolamine. Although lyso-PEs are important biomarkers in various diseases, their determination is limited by the lack of simple and efficient quantification methods. This study aims to develop an improved quantitative method for the determination of lyso-PEs and its application to an epidemiological study. METHODS: Single reaction monitoring channels by collision-induced dissociation for seven lyso-PEs were established using liquid chromatography-tandem mass spectrometry. Plasma lyso-PEs were extracted with a single-phase method using an isotopically labelled internal standard for quantification. The proposed method was adopted to define lyso-PEs in plasma samples of children aged 9-12 years living in Sapporo, Japan. RESULTS: The limit of detection and limit of quantification for each lyso-PE ranged between 0.001-0.015 and 0.002-0.031 pmol/μL, respectively. Recoveries were found to be > 91% for all the species. The analysis results of children's plasma showed that the total lyso-PE concentrations in boys (n = 181) and girls (n = 161) were 11.53 and 11.00 pmol/μL (median), respectively. Participants were further classified by the percentage of overweight and subgrouped as underweight (n = 12), normal range (n = 292), or overweight (n = 38). Interestingly, the reduction of lyso-PE 16:0 and increased lyso-PE 22:6 were observed in overweight children compared with normal range (Fold change: 0.909 and 1.174, respectively). CONCLUSIONS: This study successfully established a simple quantitative method to determine lyso-PE concentrations. Furthermore, our method revealed the possible relation between plasma lyso-PEs and overweight status.

Plasma Lipidomics of Preadolescent Children: A Hokkaido Study
Jayashankar Jayaprakash, Siddabasave Gowda B. Gowda, Divyavani Gowda, Atsuko Ikeda, Yu Ait Bamai, Rahel Mesfin Ketema, Reiko Kishi, Yifan Chen, Hitoshi Chiba, Shu-Ping Hui
Journal of Lipids, 2025, 3106145, 3106145, 2025年01月, ［国際誌］
英語, 研究論文（学術雑誌）, Lipids are the most abundant biomolecules of human plasma, and their balance plays a significant role in health and disease management. Despite the importance of lipids, the studies focused on the comprehensive determination of the plasma lipidome in children are limited. In this study, we investigated the sex, age, and weight-specific changes in the plasma lipidome of nonfasting preadolescent children aged 9-12 years (n = 342) using a nontargeted liquid chromatography-mass spectrometry technique. A total of 219 lipid species were characterized in the plasma samples. Multivariate analysis revealed that boys and girls have similar lipid profiles, but relatively higher levels of capric acid-composed triacylglycerols (TGs) were observed in plasma samples of boys. Saturated fatty acids are the most abundant fatty acyls followed by mono- and polyunsaturated fatty acids in the plasma of both boys and girls. Sphingolipids such as ceramides, hexosylceramides, sphingomyelin, and a phospholipid (phosphatidylinositol) were relatively higher in the plasma of a 10-year-old group than other age groups. Plasma levels of TG and phosphatidylserine were increased within age from 9 to 12 years. Furthermore, most of the TG molecular species were increased in the plasma of overweight children compared to the normal range groups. The receiver operating characteristic analysis results show that TG (10:0/10:0/18:1) could be a specific marker for childhood obesity (area under the curve (AUC) = 0.72). Overall, this study highlights the altered plasma lipidome in preadolescent children for sex, age, and percentage of overweight. Early detection of lipid markers for obesity would be a promising target for developing therapeutic strategies.

Targeted Circulating Lipid Mediators and Immune Cell Gene Transcripts After ST-Elevation Myocardial Infarction.
Dae Hyun Lee, Gunjan Upadhyay, Siddabasave Gowda B Gowda, Vasundhara Kain, Md Abdul Malek, Nicholas Carris, Samip Vasaiwala, Timothy Yeatman, Guilherme Oliveira, Shu-Ping Hui, Ganesh V Halade
American journal of physiology. Heart and circulatory physiology, 2024年12月20日, ［国際誌］
英語, 研究論文（学術雑誌）, Residual inflammation drives atherogenesis to atherosclerosis and myocardial infarction, which triggers acute inflammation. In preclinical studies, polyunsaturated fatty acids-derived specialized pro-resolving mediators (SPMs) have been shown to promote recovery after MI, in contrast to pro-inflammatory lipid mediators (PIMs). However, the dynamic changes of lipid mediators after ST-elevation myocardial infarction (STEMI), particularly after percutaneous coronary intervention (PCI) and respective gene transcripts, are poorly understood. Therefore, the study aimed to assess the early dynamic changes in circulating lipid mediators and lipid pathway transcripts in STEMI patients who undergo PCI. In this prospective observational clinical study, patients with STEMI (n=10) and control subjects (n=6) were included. Plasma samples for lipid mediator profiling (targeted oxylipids) and whole blood for inflammation-related transcript expression were collected at baseline before PCI, 2 hours, and 24 hours post-PCI. A total of 10 STEMI patients received PCI with a mean age of 53.3 years, 90% male. Linoleic acid and DPA levels were higher in STEMI patients. A subset of PIM levels (HETEs, PGE2) were elevated at the baseline, with a subsequent decrease in circulating levels at 2 hours after PCI (TXB2, LTB4, 20-OH-LTB4). A subset of SPM levels (HEPEs) were elevated at the baseline of STEMI suggestive overlap of inflammation-resolution signaling. The temporal kinetics of LMs showed that both the initiation of inflammation and the resolution process start simultaneously and continue as an endogenous repair mechanism during STEMI. Therefore, approaches to increase these endogenous bioactive resolution mediators content and/or efficacy before PCI should be considered in treating MI patients.

Nontargeted Lipidomics of Sorghum Grain Reveals Novel Fatty Acid Esters of Hydroxy Fatty Acids and Cultivar Differences in Lipid Profiles
Lipsa Rani Nath, Siddabasave Gowda B. Gowda, Thomas H. Roberts, Divyavani Gowda, Ali Khoddami, Shu-Ping Hui
Journal of Agricultural and Food Chemistry, 72, 37, 20690, 20703, American Chemical Society (ACS), 2024年09月04日, ［国際誌］
英語, 研究論文（学術雑誌）, Sorghum, a globally grown gluten-free cereal, is used mainly as an animal feed in developed countries regardless of its potential for human consumption. In this study, we utilized nontargeted lipidomics to thoroughly analyze, compare, and characterize whole-grain lipids in six sorghum cultivars (cv) grown in a single field trial in Australia: Buster, Bazley, Cracker, Liberty, MR43, and Tiger. In total, 194 lipid molecular species representing five major lipid classes were identified. Multivariate analysis unveiled distinct lipid profiles among the cultivars. The most distinct lipid profile belonged to cv. MR43. The lower ω-6 to ω-3 ratio and optimal P/S ratio in cv. Bazley reflect this as a valuable source of balanced essential fatty acids in the diet. The novel bioactive lipids known as FAHFAs (fatty acid esters of hydroxy fatty acids) were identified and characterized in sorghum grains. These findings further emphasize the potential of whole-grain sorghum as a basis for new health-promoting food products.

Lipidomics of herbal tea revealed their potential lipid nutrients including novel fatty acid esters of hydroxy fatty acids
Rachana M. Gangadhara, Siddabasave Gowda B. Gowda, Kota Yanagimoto, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui
Journal of Food Measurement and Characterization, Springer Science and Business Media LLC, 2024年08月30日
研究論文（学術雑誌）

Quantitative determination of plasma cholesteryl ester levels in Japanese preadolescents from the Hokkaido study using liquid chromatography/tandem mass spectrometry.
Divyavani Gowda, Siddabasave Gowda B Gowda, Atsuko Ikeda, Rahel Mesfin Ketema, Yu Ait Bamai, Reiko Kishi, Hitoshi Chiba, Shu-Ping Hui
Steroids, 211, 109498, 109498, 2024年08月13日, ［国際誌］
英語, 研究論文（学術雑誌）, Cholesteryl esters (CE) are sterols comprising various fatty acyl chains attached to a cholesterol hydroxyl moiety. CEs are often considered plasma biomarkers of liver function; however, their absolute concentrations in the plasma of Japanese preadolescents have not been well explored. This study aimed to determine the plasma CE levels in Japanese preadolescents of different sexes, ages, and body weights living in Hokkaido, Japan using targeted liquid chromatography/tandem mass spectrometry. The analysis was performed on the non-fasting plasma of preadolescents aged 9-12 years (n = 339 healthy volunteers; 178 boys and 161 girls) from Sapporo, Hokkaido, Japan. The analysis results showed that the total CE levels in boys and girls were 871 ± 153 and 862 ± 96 pmol/μL, respectively. CE 18:2 (41 ± 2.9 %) was found to be the most abundant species followed by CE 18:1 (16 ± 1.5 %) and CE 16:0 (13 ± 1.1 %). The ω-3 fatty acid-containing CEs such as CE 18:3 and CE 20:5 were significantly lower in girls than in boys. Despite the different ages, CEs were tightly regulated in the plasma of children's, and the total CEs ranged between 844 and 906 pmol/μL in boys and 824 and 875 pmol/μL in girls. The participants were further classified into three groups based on their body mass index underweight (n = 237), normal weight (n = 94), and overweight (n = 8). Most of the quantified CEs were accumulated in the overweight group. Interestingly, CE 18:3 was significantly upregulated in the overweight group compared to that in the normal range, and the area under the receiver operating characteristic curve was 0.73, suggesting that it could be a possible marker for obesity. This study marks the initial investigation of absolute CE levels in the plasma of children and can help elucidate the relationship between CEs and childhood obesity.

Lipidomic study of kidney in a mouse model with urine flow obstruction.
Divyavani Gowda, Md Abdul Masum, Siddabasave Gowda B Gowda, Chandra Shekhar, Md Zahir Uddin Rubel, Shunnosuke Kira, Osamu Ichii, Yasuhiro Kon, Hitoshi Chiba, Shu-Ping Hui
Scientific reports, 14, 1, 18042, 18042, 2024年08月05日, ［国際誌］
英語, 研究論文（学術雑誌）, Obstructed urine flow is known to cause structural and functional kidney damage leading to renal fibrosis. However, limited information is available on the change in kidney lipids during urinary tract obstruction. In this study, we investigated the change in lipidome in a mouse model with unilateral ureteral obstruction (UUO). The establishment of the UUO model was confirmed by histopathological examination using transmission electron microscopy. Untargeted liquid chromatography/mass spectrometry was carried out over a time course of 4 and 7 days. Compared to the sham control, the UUO kidney at 7 days showed dilatation of the renal tubule with loss of brush borders and thickening of the capillary endothelium. In the kidney lipidomes obtained from the UUO 7 days group compared to the control, a significant decrease of ceramide, sphingomyelin, phosphatidylcholine, lysophospholipids, and phosphatidylethanolamine was observed, whereas cholesteryl esters, free fatty acids, phosphatidylglycerol, and cardiolipins were significantly increased. The present study revealed the disturbed lipid metabolism in the UUO model, which may provide a clue to potential lipid pathways and therapeutic targets for the early stage of renal fibrosis.

Targeted and untargeted lipidomics with integration of liver dynamics and microbiome after dietary reversal of obesogenic diet targeting inflammation-resolution signaling in aging mice.
Gunjan Upadhyay, Siddabasave Gowda B Gowda, Sidharth P Mishra, Lipsa Rani Nath, Adewale James, Alisha Kulkarni, Yuktee Shrikant, Rohitram Upendram, MathanKumar Marimuthu, Shu-Ping Hui, Shalini Jain, Kain Vasundhara, Hariom Yadav, Ganesh V Halade
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1869, 8, 159542, 159542, 2024年08月02日, ［国際誌］
英語, 研究論文（学術雑誌）, Obesity, a global epidemic linked to around 4 million deaths yearly, arises from lifestyle imbalances impacting inflammation-related conditions like non-alcoholic fatty liver disease and gut dysbiosis. But the long-term effects of inflammation caused by lifestyle-related dietary changes remain unexplained. In this study, we used young male C57Bl/6 mice which were fed either an obesogenic diet (OBD) or a control diet (CON) for six months. Later, a group of mice from the OBD group were intervened to the CON diet (OBD-R) for four months, while another OBD group remained on the OBD diet. The OBD induced distinct changes in gut microbial, notably elevating Firmicutes and Actinobacteria, while reducing Bacteroidetes and Tenericutes. OBD-R restored microbial abundance like CON. Analyzing liver, plasma, and fecal samples revealed OBD-induced alterations in various structural and bioactive lipids, which were normalized to CON in the OBD-R, showcasing lipid metabolism flexibility and adaptability to dietary shifts. OBD increased omega 6 fatty acid, Arachidonic Acid (AA) and decreased omega 3-derived lipid mediators in the OBD mimicking non-alcoholic fatty liver disease thus impacting inflammation-resolution pathways. OBD also induced hepatic inflammation via increasing alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and proinflammatory markers CCR2, TNF-α, and IL-1β in liver. Transitioning from OBD to CON mitigated inflammatory gene expression and restored lipid and cholesterol networks. This study underscores the intricate interplay between lifestyle-driven dietary changes, gut microbiota, lipid metabolism, and liver health. Notably, it suggests that shift from an OBD (omega-6 enriched) to CON partially alleviates signs of chronic inflammation during aging. Understanding these microbial, lipidomic, and hepatic inflammatory dynamics reveals potential therapeutic avenues for metabolic disorders induced by diet, emphasizing the pivotal role of diet in sustaining metabolic health.

Dissecting new lipids and their composition in herbal tea using untargeted LC/MS
Lipsa Rani Nath, Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Shu Ping Hui
Food Chemistry, 447, 138941, 138941, Elsevier BV, 2024年07月, ［国際誌］
英語, 研究論文（学術雑誌）, Herbal teas and beverages have gained global attention because they are rich in natural bioactive compounds, which are known to have diverse biological effects, including antioxidant and anticarcinogenic properties. However, the lipidomic profiles of herbal teas remain unclear. In this study, we applied an untargeted lipidomics approach using high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry to comprehensively profile, compare, and identify unknown lipids in four herbal teas: dokudami, kumazasa, sugina, and yomogi. A total of 341 molecular species from five major classes of lipids were identified. Multivariate principal component analysis revealed distinct lipid compositions for each of the herbs. The fatty acid α-linolenic acid (FA 18:3) was found to be abundant in kumazasa, whereas arachidonic acid (FA 20:4) was the most abundant in sugina. Interestingly, novel lipids were discovered for the first time in plants; specifically, short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs) with 4-hydroxy phenyl nonanoic acid as the structural core. This study provides insight into the lipidomic diversity and potential bioactive lipid components of herbal teas, offering a foundation for further research into their health-promoting properties and biological significance.

Exploration of New Lipid Nutrients and Their Characterization in Herbal Teas Using Non-Targeted Liquid Chromatography-Mass Spectrometry.
Md Abdul Malek, Siddabasave Gowda B Gowda, Rachana M Gangadhara, Divyavani Gowda, Shu-Ping Hui
Foods (Basel, Switzerland), 13, 12, 2024年06月14日, ［国際誌］
英語, 研究論文（学術雑誌）, Herbal teas are blends of leaves, seeds, fruits, and flowers from various plants that provide relaxation, anti-inflammatory benefits, and immune system support for conditions such as diabetes and asthma. Despite their health benefits, comprehensive lipidomic data on herbal teas are limited in the literature. We used non-targeted liquid chromatography-linear ion trap orbitrap mass spectrometry to identify and correlate the lipid species in the following six herbal tea samples: fennel, ginger, juniper, lemon peel, orange peel, and rosehip. A total of 204 lipid molecular species were identified, and multivariate analysis revealed a significant difference between lipid species in herbal teas. Saturated fatty acids (SFAs) and polyunsaturated fatty acids (PUFAs) are significantly abundant in juniper, including ω-3 and ω-6 fatty acids, followed by fennel. Cluster correlations showed that ginger contained mainly sphingolipids and lysophospholipids, whereas fennel was rich in phospholipids. No significant variations in the content of triacylglycerols were observed in any of the herbal teas analyzed. The ratio of PUFAs to SFAs in herbal teas showed that orange peel had the highest ratio, followed by lemon peel and fennel, indicating their potential health benefits. In addition, using high-resolution mass spectrometry, various lipids such as fatty acid esters of hydroxy fatty acids and N-acyl-lysophosphatidylethanolamines were identified and characterized in these herbal teas. This study provides a comprehensive lipid analysis and detailed characterization of lipids in six herbal teas, highlighting their plausible applications in the field of nutrition and various food industries for the development of functional foods.

Alterations in plasma short-chain fatty acids in preadolescence children: The Hokkaido study
Yonghan Li, Siddabasave Gowda B. Gowda, Divyavani Gowda, Atsuko Ikeda, Yu Ait Bamai, Rahel Mesfin Ketema, Reiko Kishi, Hitoshi Chiba, Shu-Ping Hui
Journal of Chromatography B, 1242, 124191, 124191, Elsevier BV, 2024年06月, ［国際誌］
英語, 研究論文（学術雑誌）, The purpose of this study is to explore the plasma short-chain fatty acid (SCFA) concentrations in 9-12-year-old Japanese children collected in the Hokkaido study, focusing on how factors such as age, sex, and body mass index (BMI) correlate with these levels. The Hokkaido Study on Children's Health is an ongoing longitudinal study since 2002, encompassing 20,926 pregnant women in Hokkaido Prefecture, Japan, between 2003 and 2012. We contacted 1881 children aged 9-12 born between April 2006 and January 2010, and 342 non-fasting plasma samples (boys = 181, girls = 161) were obtained from this cohort, alongside assessments of their height and weight. Plasma SCFA concentrations were determined using N,N-dimethylethylenediamine derivatization method coupled with liquid chromatography-mass spectrometry. Ethyl acetate was used to extract SCFAs from plasma, and the recovery ranged from 83 % to 108 %. Our findings indicate that acetic acid had the highest concentration across all age groups and sexes. The concentrations of butyric acid, valeric acid, and hexanoic acid increased with age, peaking in 12-year-old children. Conversely, the level of 4-hydroxy valeric acid showed a decreasing trend with increasing age groups. This study also explored the correlation between BMI and SCFA concentrations, comparatively higher level of propionic acid was observed in the overweight group. The results obtained in this study enhance our understanding of the role of SCFAs in the growth and development of children and provide a foundation for future nutritional intervention and health promotion strategies.

Corrigendum to "Dissecting new lipids and their composition in herbal tea using untargeted LC/MS" [Food Chemistry, 447 (2024), 138941].
Lipsa Rani Nath, Siddabasave Gowda B Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Shu-Ping Hui
Food chemistry, 453, 139745, 139745, 2024年05月24日, ［国際誌］
英語

Regio-specific lipid fingerprinting of edible sea cucumbers using LC/MS.
Yusuke Minami, Siddabasave Gowda B Gowda, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui
Food research international (Ottawa, Ont.), 184, 114253, 114253, 2024年05月, ［国際誌］
英語, 研究論文（学術雑誌）, Sea cucumbers are a rich source of bioactive compounds and are gaining popularity as nutrient-rich seafood. They are consumed as a whole organism in Pacific regions. However, limited data are available on the comparison of their lipid composition and nutritional value. In this study, untargeted liquid chromatography/mass spectrometry was applied to comprehensively profile lipids in the skin, meat, and intestinal contents of three color-distinct edible sea cucumbers. Multivariate principal component analysis revealed that the lipid composition of the intestinal contents of red, black, and blue sea cucumbers differs from that of skin, and meats. Polyunsaturated fatty acids (PUFAs) are abundant in the intestinal contents, followed by meats of sea cucumber. Lipid nutritional quality assessments based on fatty acid composition revealed a high P:S ratio, low index of atherogenicity, and high health promotion indices for the intestinal contents of red sea cucumber, suggesting its potential health benefits. In addition, hierarchical cluster analysis revealed that the intestinal contents of sea cucumbers were relatively high in PUFA-enriched phospholipids and lysophospholipids. Ceramides are abundant in black skin, blue meat, and red intestinal content samples. Overall, this study provides the first insights into a comprehensive regio-specific profile of the lipid content of sea cucumbers and their potential use as a source of lipid nutrients in food and nutraceuticals.

Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS
Jayashankar Jayaprakash, Siddabasave Gowda B. Gowda, Pradeep K. Shukla, Divyavani Gowda, Lipsa Rani Nath, Hitoshi Chiba, Radhakrishna Rao, Shu-Ping Hui
ACS Omega, 9, 14, 16044, 16054, 2024年04月09日, ［国際誌］
英語, 研究論文（学術雑誌）, Consumption of alcohol has widespread effects on the human body. The organs that are most significantly impacted are the liver and digestive system. When alcohol is consumed, it is absorbed in the intestines and processed by the liver. However, excessive alcohol use may affect gut epithelial integrity, microbiome composition, and lipid metabolism. Despite past studies investigating the effect of ethanol on hepatic lipid metabolism, the focus on colonic lipid metabolism has not been well explored. In this study, we investigated the sex-specific effect of ethanol on the colonic content lipidome in a mouse model using nontargeted liquid chromatography-mass spectrometry. Comprehensive lipidome analysis of colonic flush samples was performed using ethanol-fed (EF) and pair-fed (PF) mice of each sex. Partial least-squares discriminant analysis revealed that ethanol altered colonic lipid composition largely in male mice compared with female mice. A significant increase in free fatty acids, ceramides, and hexosylceramides and decreased phosphatidylglycerols (PG) was observed in the EF group compared to the PF group in male mice. Phosphatidylethanolamine (PE) levels were increased significantly in the EF group of both sexes compared to the PF group. The volcanic plot shows that PG (O-15:1/15:0) and PE (O-18:2/15:0) are common markers that are increased in both sexes of the EF group. In addition, decreased fatty acid esters of hydroxy fatty acids (FAHFA) were observed specifically in the EF group of female mice. Overall, a significant variation in the mice colonic content lipidome between the EF and PF groups was observed. Target pathways, such as sphingolipid metabolism in males, FAHFA in females, and PE metabolism in both sexes, were suggested. This study provides new insight into the sex-dependent lipid change associated with alcohol-induced gut-microbiota dysfunction and its potential health impacts.

Glucosylceramide flippases contribute to cellular glucosylceramide homeostasis
Natsuki Kita, Asuka Hamamoto, Siddabasave Gowda B. Gowda, Hiroyuki Takatsu, Kazuhisa Nakayama, Makoto Arita, Shu-Ping Hui, Hye-Won Shin
Journal of Lipid Research, 65, 3, 100508, 100508, 2024年03月, ［国際誌］
英語, 研究論文（学術雑誌）, Lipid transport is an essential cellular process with importance to human health, disease development, and therapeutic strategies. Type IV P-type ATPases (P4-ATPases) have been identified as membrane lipid flippases by utilizing nitrobenzoxadiazole (NBD)-labeled lipids as substrates. Among the 14 human type IV P-type ATPases, ATP10D was shown to flip NBD-glucosylceramide (GlcCer) across the plasma membrane. Here, we found that conversion of incorporated GlcCer (d18:1/12:0) to other sphingolipids is accelerated in cells exogenously expressing ATP10D but not its ATPase-deficient mutant. These findings suggest that 1) ATP10D flips unmodified GlcCer as well as NBD-GlcCer at the plasma membrane and 2) ATP10D can translocate extracellular GlcCer, which is subsequently converted to other metabolites. Notably, exogenous expression of ATP10D led to the reduction in cellular hexosylceramide levels. Moreover, the expression of GlcCer flippases, including ATP10D, also reduced cellular hexosylceramide levels in fibroblasts derived from patients with Gaucher disease, which is a lysosomal storage disorder with excess GlcCer accumulation. Our study highlights the contribution of ATP10D to the regulation of cellular GlcCer levels and maintaining lipid homeostasis.

Analysis of lipid composition and characterization of acyl steryl glycosides in rose petals by using nontargeted LC/MS
Md Abdul Malek, Siddabasave Gowda B. Gowda, Divyavani Gowda, Shu-Ping Hui
Journal of Food Measurement and Characterization, 2024年03月
研究論文（学術雑誌）

A facile method for monitoring sphingomyelin synthase activity in HeLa cells using liquid chromatography/mass spectrometry
Punith M. Sundaraswamy, Yusuke Minami, Jayashankar Jayaprakash, Siddabasave Gowda B. Gowda, Hiroyuki Takatsu, Divyavani Gowda, Hye-Won Shin, Shu-Ping Hui
The Analyst, 149, 12, 3293, 3301, 2024年, ［国際誌］
英語, 研究論文（学術雑誌）, Sphingomyelin synthase (SMS) is a sphingolipid-metabolizing enzyme involved in the de novo synthesis of sphingomyelin (SM) from ceramide (Cer). Recent studies have indicated that SMS is a key therapeutic target for metabolic diseases such as fatty liver, type 2 diabetes, atherosclerosis, and colorectal cancer. However, very few SMS inhibitors have been identified because of the limited sensitivity and selectivity of the current fluorescence-based screening assay. In this study, we developed a simple cell-based assay coupled with liquid chromatography/tandem mass spectrometry (LC-MS/MS) to screen for SMS inhibitors. HeLa cells stably expressing SMS1 or SMS2 were used for the screening. A non-fluorescent unnatural C6-Cer was used as a substrate for SMS to produce C6-SM. C6-Cer and C6-SM levels in the cells were monitored and quantified using LC-MS/MS. The activity of ginkgolic acid C15:1 (GA), a known SMS inhibitor, was measured. GA had half-maximal inhibitory concentrations of 5.5 μM and 3.6 μM for SMS1 and SMS2, respectively. To validate these findings, hSMS1 and hSMS2 proteins were optimized for molecular docking studies. In silico analyses were conducted to assess the interaction of GA with SMS1 and SMS2, and its binding affinity. This study offers an analytical approach for screening novel SMS inhibitors and provides in silico support for the experimental findings.

Regiospecific analysis of lipidome in the brain from mammals of land and aquatic habitats-by liquid chromatography-mass spectrometry
Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Chandra Shekhar, Hitoshi Chiba, Nina Patzke, Shu-Ping Hui
Heliyon, 9, 12, e22959, e22959, Elsevier BV, 2023年12月, ［国際誌］
英語, 研究論文（学術雑誌）, The brain is a complex organ demonstrated by the occurrence of specific types of functional lipids. Despite some studies focusing on providing the animal brain lipid signature, there are limited studies focusing on the comprehensive and regiospecific characterization of multiple animal brain lipidome. Herein we characterized about 294 lipid molecular species from six different lipid classes in different portions of the brain after fixation from mammals of different habitats, fully-aquatic (n = 6), semi-aquatic (n = 6), and terrestrial (n = 4), using liquid chromatography-mass spectrometry. The untargeted brain lipid profiling revealed a significant difference in total lipid levels between fully-aquatic, semi-aquatic, and terrestrial mammals. The polyunsaturated fatty acids and cholesterol esters are abundant in brain tissue of semi-aquatic followed by fully-aquatic mammals whereas phosphatidylethanolamines are profoundly high in terrestrial species. The regiospecific analysis revealed a predominance of sphingolipids in all the groups but no significant differences were observed between the different portions of the brain such as the cerebellum, cortex, pons, spinal cord, and thalamus. Interestingly the multivariate analysis showed almost the same lipid compositions in the spinal cord and thalamus of terrestrial mammals. Overall, this is the first report to compare the comprehensive brain-lipidome among different mammalian groups inhabiting three distinct habitats. These results indicate that the brain lipid composition is specific to the animal habitat.

Crosstalk between Lipids and Non-Alcoholic Fatty Liver Disease
Divyavani Gowda, Chandra Shekhar, Siddabasave Gowda B. Gowda, Yifan Chen, Shu-Ping Hui
Livers, 3, 4, 687, 708, MDPI AG, 2023年11月23日
研究論文（学術雑誌）, Non-alcoholic fatty liver disease (NAFLD), a complex liver disorder that can result in non-alcoholic steatohepatitis, cirrhosis, and liver cancer, is the accumulation of fat in the liver seen in people due to metabolic dysfunction. The pathophysiology of NAFLD is influenced by several variables, such as metabolic dysregulation, oxidative stress, inflammation, and genetic susceptibility. This illness seriously threatens global health because of its link to obesity, insulin resistance, type 2 diabetes, and other metabolic disorders. In recent years, lipid–NAFLD crosstalk has drawn a lot of interest. Through numerous methods, lipids have been connected to the onset and advancement of the illness. The connection between lipids and NAFLD is the main topic of the current review, along with the various therapeutic targets and currently available drugs. The importance of hepatic lipid metabolism in the progression of NAFLD is summarized with the latest results in the field.

The elucidation of plasma lipidome profiles during severe influenza in a mouse model.
Marumi Ohno, Siddabasave Gowda B Gowda, Toshiki Sekiya, Naoki Nomura, Masashi Shingai, Shu-Ping Hui, Hiroshi Kida
Scientific reports, 13, 1, 14210, 14210, 2023年08月30日, ［国際誌］
英語, 研究論文（学術雑誌）, Although influenza virus infection has been shown to affect lipid metabolism, details remain unknown. Therefore, we elucidated the kinetic lipid profiles of mice infected with different doses of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) by measuring multiple lipid molecular species using untargeted lipidomic analysis. C57BL/6 male mice were intranasally infected with PR8 virus at 50 or 500 plaque-forming units to cause sublethal or lethal influenza, respectively. Plasma and tissue samples were collected at 1, 3, and 6 days post-infection (dpi), and comprehensive lipidomic analysis was performed using high-performance liquid chromatography-linear trap quadrupole-Orbitrap mass spectrometry, as well as gene expression analyses. The most prominent feature of the lipid profile in lethally infected mice was the elevated plasma concentrations of phosphatidylethanolamines (PEs) containing polyunsaturated fatty acid (PUFA) at 3 dpi. Furthermore, the facilitation of PUFA-containing phospholipid production in the lungs, but not in the liver, was suggested by gene expression and lipidomic analysis of tissue samples. Given the increased plasma or serum levels of PUFA-containing PEs in patients with other viral infections, especially in severe cases, the elevation of these phospholipids in circulation could be a biomarker of infection and the severity of infectious diseases.

Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS.
Rachana M Gangadhara, Siddabasave Gowda B Gowda, Divyavani Gowda, Ken Inui, Shu-Ping Hui
Foods (Basel, Switzerland), 12, 14, 2023年07月21日, ［国際誌］
英語, 研究論文（学術雑誌）, Beans, a globally significant economic and nutritional food crop, are rich in polyphenolic chemicals with potential health advantages, providing high protein, fiber, minerals, and vitamins. However, studies on the global profiling of lipids in beans are limited. We applied a non-targeted lipidomic approach based on high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (HPLC/LTQ-Orbitrap-MS) to comprehensively profile and compare the lipids in six distinct bean cultivars, namely, adzuki red beans-adzuki cultivar (ARB-AC), adzuki red beans-Benidainagon cultivar (ARB-BC), adzuki red beans-Erimoshouzu cultivar (ARB-EC), soybean-Fukuyutaka cultivar 2021 (SB-FC21), soybean-Fukuyutaka cultivar 2022 (SB-FC22), and soybean-Oosuzu cultivar (SB-OC). MS/MS analysis defined 144 molecular species from four main lipid groups. Multivariate principal component analysis indicated unique lipid compositions in the cultivars except for ARB-BC and ARB-EC. Evaluation of the concentrations of polyunsaturated fatty acid to saturated fatty acid ratio among all the cultivars showed that SB-FC21 and SB-FC22 had the highest value, suggesting they are the most beneficial for health. Furthermore, lipids such as acyl sterol glycosides were detected and characterized for the first time in these bean cultivars. Hierarchical cluster correlations revealed the predominance of ceramides in ARB-EC, lysophospholipids in SB-FC21, and glycerophospholipids in SB-OC. This study comprehensively investigated lipids and their compositions in beans, indicating their potential utility in the nutritional evaluation of beans as functional foods.

Sleep deprivation in obesogenic setting alters lipidome and microbiome toward suboptimal inflammation in acute heart failure.
Ganesh V Halade, Yusuf Mat, Siddabasave Gowda B Gowda, Shalini Jain, Shu-Ping Hui, Hariom Yadav, Vasundhara Kain
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 37, 5, e22899, 2023年05月, ［国際誌］
英語, 研究論文（学術雑誌）, Sleep is a fundamental medicine for cardiac homeostasis, and sleep-deprived individuals are prone to higher incidences of heart attack. The lipid-dense diet (obesogenic diet-OBD) is a cumulative risk factor for chronic inflammation in cardiovascular disease; thus, understanding how sleep fragmentation (SF) in an obesity setting impacts immune and cardiac health is an unmet medical need. We hypothesized whether the co-existence of SF with OBD dysregulates gut homeostasis and leukocyte-derived reparative/resolution mediators, thereby impairing cardiac repair. Two-month-old male C57BL/6J mice were randomized first into two groups, then four groups; Control, control + SF, OBD, and OBD + SF mice subjected to myocardial infarction (MI). OBD mice had higher levels of plasma linolenic acid with a decrease in eicosapentaenoic and docosahexaenoic acid. The OBD mice had lower Lactobacillus johnsonii indicating a loss of probiotic microbiota. SF in OBD mice increased Firmicutes/Bacteroidetes ratio indicative of a detrimental change in SF-directed microbiome. OBD + SF group increased in the neutrophil: lymphocyte ratio suggestive of suboptimal inflammation. As a result of SF, resolution mediators (RvD2, RvD3, RvD5, LXA4 , PD1, and MaR1) decreased and inflammatory mediators (PGD2 , PGE2 , PGF2a , 6k-PGF1a ) were increased in OBD mice post-MI. At the site of infarction, the proinflammatory cytokines Ccl2, IL1β, and IL-6 were amplified in OBD + SF indicating a robust proinflammatory milieu post-MI. Also, brain circadian genes (Bmal1, Clock) were downregulated in SF-subjected control mice, but remained elevated in OBD mice post-MI. SF superimposed on obesity dysregulated physiological inflammation and disrupted resolving response thereby impaired cardiac repair and signs of pathological inflammation.

Mass spectrometric approaches in discovering lipid biomarkers for COVID-19 by lipidomics: Future challenges and perspectives.
Siddabasave Gowda B Gowda, Chandra Shekhar, Divyavani Gowda, Yifan Chen, Hitoshi Chiba, Shu-Ping Hui
Mass spectrometry reviews, 43, 5, 1041, 1065, Wiley, 2023年04月27日, ［国際誌］
英語, 研究論文（学術雑誌）, Coronavirus disease 2019 (COVID-19) has emerged as a global health threat and has rapidly spread worldwide. Significant changes in the lipid profile before and after COVID-19 confirmed the significance of lipid metabolism in regulating the response to viral infection. Therefore, understanding the role of lipid metabolism may facilitate the development of new therapeutics for COVID-19. Owing to their high sensitivity and accuracy, mass spectrometry (MS)-based methods are widely used for rapidly identifying and quantifying of thousands of lipid species present in a small amount of sample. To enhance the capabilities of MS for the qualitative and quantitative analysis of lipids, different platforms have been combined to cover a wide range of lipidomes with high sensitivity, specificity, and accuracy. Currently, MS-based technologies are being established as efficient methods for discovering potential diagnostic biomarkers for COVID-19 and related diseases. As the lipidome of the host cell is drastically affected by the viral replication process, investigating lipid profile alterations in patients with COVID-19 and targeting lipid metabolism pathways are considered to be crucial steps in host-directed drug targeting to develop better therapeutic strategies. This review summarizes various MS-based strategies that have been developed for lipidomic analyzes and biomarker discoveries to combat COVID-19 by integrating various other potential approaches using different human samples. Furthermore, this review discusses the challenges in using MS technologies and future perspectives in terms of drug discovery and diagnosis of COVID-19.

Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease
Asuka Hamamoto, Natsuki Kita, Siddabasave Gowda B Gowda, Hiroyuki Takatsu, Kazuhisa Nakayama, Makoto Arita, Shu-Ping Hui, Hye-Won Shin
Cell Structure and Function, 49, 1, 1, 10, Japan Society for Cell Biology, 2023年, ［国内誌］
英語, 研究論文（学術雑誌）, Gaucher disease (GD) is a recessively inherited lysosomal storage disorder characterized by a deficiency of lysosomal glucocerebrosidase (GBA1). This deficiency results in the accumulation of its substrate, glucosylceramide (GlcCer), within lysosomes. Here, we investigated lysosomal abnormalities in fibroblasts derived from patients with GD. It is noteworthy that the cellular distribution of lysosomes and lysosomal proteolytic activity remained largely unaffected in GD fibroblasts. However, we found that lysosomal membranes of GD fibroblasts were susceptible to damage when exposed to a lysosomotropic agent. Moreover, the susceptibility of lysosomal membranes to a lysosomotropic agent could be partly restored by exogenous expression of wild-type GBA1. Here, we report that the lysosomal membrane integrity is altered in GD fibroblasts, but lysosomal distribution and proteolytic activity is not significantly altered.Key words: glucosylceramide, lysosome, Gaucher disease, lysosomotropic agent.

Temporal lipid profiling in the progression from acute to chronic heart failure in mice and ischemic human hearts
Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Vibhu Parcha, Pankaj Arora, Ganesh V. Halade, Shu-Ping Hui
Atherosclerosis, 363, 30, 41, Elsevier BV, 2022年12月, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND AND AIMS: Myocardial infarction (MI) is a leading cause of heart failure (HF). After MI, lipids undergo several phasic changes implicated in cardiac repair if inflammation resolves on time. However, if inflammation continues, that leads to end stage HF progression and development. Numerous studies have analyzed the traditional risk factors; however, temporal lipidomics data for human and animal models are limited. Thus, we aimed to obtain sequential lipid profiling from acute to chronic HF. METHODS: Here, we report the comprehensive lipidome of the hearts from diseased and healthy subjects. To induce heart failure in mice, we used a non-reperfused model of coronary ligation, and MI was confirmed by echocardiography and histology, then temporal kinetics of lipids in different tissues (heart, spleen, kidney), and plasma was quantitated from heart failure mice and compared with naïve controls. For lipid analysis in mouse and human samples, untargeted liquid chromatography-linear trap quadrupole orbitrap mass spectrometry (LC-LTQ-Orbitrap MS) was performed. RESULTS: In humans, multivariate analysis revealed distinct cardiac lipid profiles between healthy and ischemic subjects, with 16 lipid species significantly downregulated by 5-fold, mainly phosphatidylethanolamines (PE), in the ischemic heart. In contrast, PE levels were markedly increased in mouse tissues and plasma in chronic MI, indicating possible cardiac remodeling. Further, fold change analysis revealed site-specific lipid biomarkers for acute and chronic HF. A significant decrease in sulfatides (SHexCer (34:1; 2O)) and sphingomyelins (SM (d18:1/16:0)) was observed in mouse tissues and plasma in chronic HF. CONCLUSIONS: Overall, a significant decreased lipidome in human ischemic LV and differential lipid metabolites in the transition of acute to chronic HF with inter-organ communication could provide novel insights into targeting integrative pathways for the early diagnosis or development of novel therapeutics to delay/prevent HF.

Detection and characterization of lipids in eleven species of fish by non-targeted liquid chromatography/mass spectrometry
Siddabasave Gowda B. Gowda, Yusuke Minami, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui
Food Chemistry, 393, 133402, 133402, Elsevier BV, 2022年11月, ［国際誌］
英語, 研究論文（学術雑誌）, Fish is an important nutrition source because its lipids, which are rich in ω-3 fatty acids, are beneficial for human health. However, studies focusing on their detection, composition, and nutritional value are limited. In this study, we applied a non-targeted lipidomic approach based on ultra-high performance liquid chromatography coupled with linear-ion trap-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap-MS) to comprehensively profile, compare, and detect unknown lipids in eleven types of dietary fish. A total of 287 molecular species from five major lipid classes were characterized by MS/MS analysis. Multivariate principal component analysis revealed the distinct lipid composition in shishamo smelt and Japanese sardine compared to other fish types. The assessment of nutritional indices based on the levels of free fatty acid suggested that among the eleven fish types, shishamo smelt is highly beneficial for health. Further, lipids such as N-acyl lysophosphatidylethanolamine were detected and characterized for the first time in fish fillets. Hierarchical cluster correlations indicated the predominance of glycerophospholipids (GPs) and sphingolipids in sardine, whereas fatty acyls and triacylglycerols (TAGs) were predominant in shishamo smelt. The high levels of polyunsaturated fatty acid-enriched GPs and TAGs in dietary fish endow it with great potential as a health-promoting food for human consumption. This study offers a comprehensive analysis of lipids and their compositions in fish fillets, demonstrating their potential use in the nutritional assessment of functional foods.

Analysis of Antioxidant Lipids in Five Species of Dietary Seaweeds by Liquid Chromatography/Mass Spectrometry
Siddabasave Gowda B. Gowda, Chen Yifan, Divyavani Gowda, Yui Tsuboi, Hitoshi Chiba, Shu-Ping Hui
Antioxidants, 11, 8, 1538, 1538, MDPI AG, 2022年08月08日, ［国際誌］
英語, 研究論文（学術雑誌）, Seaweeds are a good source of bioactive lipids and are known for their nutritional benefits, making them a valuable food source. Despite their dietary significance and nutritional importance, there are limited reports on comprehensive lipidome analysis of lipids with antioxidant properties. Therefore, this study aimed to compare the lipid profiles of five commonly consumed Japanese dietary seaweeds using non-targeted liquid chromatography/mass spectrometry (LC/MS). A total, of 304 molecular species from four major lipid classes were detected and characterized by MS/MS analysis. Multivariate statistical analysis revealed distinct lipid molecular compositions in kombu and sea mustard compared to hijiki, mozuku, and laver seaweeds. Kombu has been shown to contain large amounts of antioxidants, such as polyunsaturated fatty acids (PUFAs), and a high health promotion index compared to other seaweeds. Hierarchical cluster correlations indicated the predominance of glycerophospholipids (GPs) and glycerolipids (GLs) in sea mustard and kombu. As a result, dietary seaweeds have great potential as antioxidants and health-promoting foods for human consumption due to their high levels of PUFA-rich GPs and GLs. Unsaturated triacylglycerols are predominant in hijiki, whereas other health-beneficial lipids, such as monogalactosyldiacylglycerol and sulfoquinovosyl diacylglycerols, are predominant in sea mustard. This study provides a detailed characterization of lipids and their comparative fingerprints in seaweeds, demonstrating the potential use of dietary seaweeds in biotechnological and industrial applications involving the development of functional food products.

Determination of short-chain fatty acids by N,N-dimethylethylenediamine derivatization combined with liquid chromatography/mass spectrometry and their implication in influenza virus infection.
Divyavani Gowda, Yonghan Li, Siddabasave Gowda B Gowda, Marumi Ohno, Hitoshi Chiba, Shu-Ping Hui
Analytical and bioanalytical chemistry, 414, 22, 6419, 6430, 2022年07月16日, ［国際誌］
英語, 研究論文（学術雑誌）, Short-chain fatty acids (SCFAs) are the end products of the fermentation of complex carbohydrates by the gut microbiota. Although SCFAs are recognized as important markers to elucidate the link between gut health and disease, it has been difficult to analyze SCFAs with mass spectrometry technologies due to their poor ionization efficiency and high volatility. Here, we present a novel and sensitive method for the quantification of SCFAs, including C2-C6 SCFAs and their hydroxy derivatives, by liquid chromatography/tandem mass spectrometry (LC-MS/MS) upon N,N-dimethylethylenediamine (DMED) derivatization with a run time of 10 min. Moreover, the quantification method of DMED-derivatized SCFAs in intestinal contents using isotope-labeled internal standards was also established. The method validation was performed by analyzing spiked intestinal samples; the limits of detection and quantification of SCFAs with this method were found to be 0.5 and 5 fmol, respectively; the recovery was greater than 80% and good linearity (0.9932 to 0.9979) of calibration curves was obtained over the range from 0.005 to 5000 pmol/μL; the intraday and interday precisions were achieved in the range of 1-5%. Furthermore, the validated method was applied to analyze SCFAs in the cecum and colon contents of mice infected with the influenza virus. The results showed that the concentration of most of the SCFAs tested here decreased significantly in a time-dependent manner after the infection, suggesting a possibility that SCFAs in intestinal samples could be used as severe disease markers. Overall, we here successfully developed a simple, fast, and sensitive method for SCFA analysis by LC-MS/MS combined with DMED derivatization. The method for the quantification of SCFAs will be a useful tool for both basic research and clinical studies.

Lipid fingerprinting of yellow mealwormTenebrio molitor by untargeted liquid chromatography-mass spectrometry
S. G. B. Gowda, Y. Sasaki, E. Hasegawa, H. Chiba, S. P. Hui
Journal of Insects as Food and Feed, 8, 2, 157, 168, Wageningen Academic Publishers, 2022年02月01日, ［査読有り］, ［筆頭著者］
研究論文（学術雑誌）, Insects such as Tenebrio molitor have been considered an alternative source of nutrition for animals and have also been adopted as human food throughout history, especially in Asia and Africa. Lipids are the second most abundant component followed by proteins. However, studies focusing on comprehensive lipid composition analsysis of these widely reared species are limited. The untargeted lipidomic analysis of yellow mealworm larvae (T. molitor) led to the identification of several lipid molecular species from lipid classes such as: free fatty acids, sphingolipids, phospholipids, and triacylglycerols. The results revealed that polyunsaturated fatty acids (PUFAs) (45%) are the most abundant fatty acids, followed by monounsaturated fatty acids (MUFAs) (42%) and saturated fatty acids (13%). Fatty acids such as FA 18:1 and FA 18:2 are the most abundant fatty acids and are substantially enriched in other complex lipids in the form of esters. Moreover, functional lipids such as sphingomyelins, ceramides, cardiolipins, phosphatidylinositols, and phosphatidylethanolamines were characterised for the first time, with a large number of MUFAs and PUFAs as their main acyl chains. Overall, our data showed the occurrence of multiple structurally diverse lipids in T. molitor, suggesting that mealworms are not only enriched with proteins but also have several functional lipids, which are highly beneficial to human and animal health. Thus, the larvae of T. molitor could serve as a promising candidate for the development of functional food and feed products.

Group IIA secreted phospholipase A2 controls skin carcinogenesis and psoriasis by shaping the gut microbiota
Yoshimi Miki, Yoshitaka Taketomi, Yuh Kidoguchi, Kei Yamamoto, Kazuaki Muramatsu, Yasumasa Nishito, Jonguk Park, Koji Hosomi, Kenji Mizuguchi, Jun Kunisawa, Tomoyoshi Soga, Eric Boilard, Siddabasave Gowda B. Gowda, Kazutaka Ikeda, Makoto Arita, Makoto Murakami
JCI Insight, 7, 2, American Society for Clinical Investigation, 2022年01月25日, ［国際誌］
英語, 研究論文（学術雑誌）, Besides promoting inflammation by mobilizing lipid mediators, group IIA secreted phospholipase A2 (sPLA2-IIA) prevents bacterial infection by degrading bacterial membranes. Here, we show that, despite the restricted intestinal expression of sPLA2-IIA in BALB/c mice, its genetic deletion leads to amelioration of cancer and exacerbation of psoriasis in distal skin. Intestinal expression of sPLA2-IIA is reduced after treatment with antibiotics or under germ-free conditions, suggesting its upregulation by gut microbiota. Metagenome, transcriptome, and metabolome analyses have revealed that sPLA2-IIA deficiency alters the gut microbiota, accompanied by notable changes in the intestinal expression of genes related to immunity and metabolism, as well as in the levels of various blood metabolites and fecal bacterial lipids, suggesting that sPLA2-IIA contributes to shaping of the gut microbiota. The skin phenotypes in Pla2g2a-/- mice are lost (a) when they are cohoused with littermate WT mice, resulting in the mixing of the microbiota between the genotypes, or (b) when they are housed in a more stringent pathogen-free facility, where Pla2g2a expression in WT mice is low and the gut microbial compositions in both genotypes are nearly identical. Thus, our results highlight a potentially new aspect of sPLA2-IIA as a modulator of gut microbiota, perturbation of which affects distal skin responses.

Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry
Chongsheng Liang, Siddabasave Gowda B. Gowda, Divyavani Gowda, Toshihiro Sakurai, Iku Sazaki, Hitoshi Chiba, Shu-Ping Hui
Antioxidants, 11, 2, 229, 229, MDPI AG, 2022年01月25日, ［国際誌］
英語, 研究論文（学術雑誌）

Defining the kinetic effects of infection with influenza virus A/PR8/34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC/MS
Divyavani Gowda, Marumi Ohno, Siddabasave Gowda B. Gowda, Hitoshi Chiba, Masashi Shingai, Hiroshi Kida, Shu-Ping Hui
Scientific Reports, 11, 1, 20161, 20161, Springer Science and Business Media LLC, 2021年12月, ［国際誌］
英語, 研究論文（学術雑誌）, Influenza remains a world-wide health concern, causing 290,000–600,000 deaths and up to 5 million cases of severe illnesses annually. Noticing the host factors that control biological responses, such as inflammatory cytokine secretion, to influenza virus infection is important for the development of novel drugs. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite and has essential biological functions in inflammation. However, the kinetic effects of influenza virus infection on physiological S1P levels and their signaling in multiple tissues remain unknown. In this study, we utilized a mouse model intranasally infected with 50 or 500 plaque forming units (PFU) of A/Puerto Rico/8/34 (H1N1; PR8) virus to investigate how S1P levels and expression of its regulating factors are affected by influenza virus infection by the liquid-chromatography/mass spectrometry and real-time PCR, respectively. The S1P level was significantly high in the plasma of mice infected with 500 PFU of the virus than that in control mice at 6 day-post-infection (dpi). Elevated gene expression of sphingosine kinase-1 (Sphk1), an S1P synthase, was observed in the liver, lung, white adipose tissue, heart, and aorta of infected mice. This could be responsible for the increased plasma S1P levels as well as the decrease in the hepatic S1P lyase (Sgpl1) gene in the infected mice. These results indicate modulation of S1P-signaling by influenza virus infection. Since S1P regulates inflammation and leukocyte migration, it must be worth trying to target this signaling to control influenza-associated symptoms.

Sphingosine-1-phosphate interactions in the spleen and heart reflect extent of cardiac repair in mice and failing human hearts
SiddabasaveGowda B. Gowda, Divyavani Gowda, Vasundhara Kain, Hitoshi Chiba, Shu-Ping Hui, Charles E. Chalfant, Vibhu Parcha, Pankaj Arora, Ganesh V. Halade
American Journal of Physiology-Heart and Circulatory Physiology, 321, 3, H599, H611, American Physiological Society, 2021年09月01日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Previous studies indicate that sphingosine-1-phosphate (S1P) has some role in cardiovascular disease. This study adds quantitative and integrative systems-based approaches that are necessary for discovery and bedside translation. Here, we quantitated sphinganine, sphingosine, sphingosine-1-phosphate (S1P) in mice and human cardiac pathobiology. Interorgan S1P quantity and respective systems-based receptor activation suggest cardiac repair after myocardial infarction. Thus, S1P serves as a therapeutic target for cardiac protection in clinical translation.

Docosahexaenoic Acid Esters of Hydroxy Fatty Acid Is a Novel Activator of NRF2
Siddabasave Gowda B. Gowda, Takayuki Tsukui, Hirotoshi Fuda, Yusuke Minami, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui
International Journal of Molecular Sciences, 22, 14, 7598, 7598, MDPI AG, 2021年07月15日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with interesting physiological functions in mammals. Despite their structural diversity and links with nuclear factor erythroid 2-related factor 2 (NRF2) biosynthesis, FAHFAs are less explored as NRF2 activators. Herein, we examined for the first time the synthetic docosahexaenoic acid esters of 12-hydroxy stearic acid (12-DHAHSA) or oleic acid (12-DHAHOA) against NRF2 activation in cultured human hepatoma-derived cells (C3A). The effect of DHA-derived FAHFAs on lipid metabolism was explored by the nontargeted lipidomic analysis using liquid chromatography-mass spectrometry. Furthermore, their action on lipid droplet (LD) oxidation was investigated by the fluorescence imaging technique. The DHA-derived FAHFAs showed less cytotoxicity compared to their native fatty acids and activated the NRF2 in a dose-dependent pattern. Treatment of 12-DHAHOA with C3A cells upregulated the cellular triacylglycerol levels by 17-fold compared to the untreated group. Fluorescence imaging analysis also revealed the suppression of the degree of LDs oxidation upon treatment with 12-DHAHSA. Overall, these results suggest that DHA-derived FAHFAs as novel and potent activators of NRF2 with plausible antioxidant function.

Detection and Structural Characterization of SFAHFA Homologous Series in Mouse Colon Contents by LTQ-Orbitrap-MS and Their Implication in Influenza Virus Infection
Siddabasave Gowda B. Gowda, Divyavani Gowda, Marumi Ohno, Chongsheng Liang, Hitoshi Chiba, Shu-Ping Hui
Journal of the American Society for Mass Spectrometry, 32, 8, 2196, 2205, American Chemical Society (ACS), 2021年06月25日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with promising physiological functions in mammals. We previously introduced a new type of lipids to this family called short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), branching specific to the C2 carbon of a long-chain fatty acid (≥C20). In this study, we discovered a homologous series of SFAHFAs comprising C16-C26 hydroxy fatty acids esterified with short-chain fatty acids (C2-C5) in mouse colon contents. The detected SFAHFAs were characterized by high-resolution mass spectrometry with MSn analysis. The double-bond position of monounsaturated SFAHFAs was determined by the epoxidation reaction of samples with m-chloroperoxybenzoic acid and their MSn analysis. Further, the measurement of SFAHFA concentration in the colon contents of mice infected with influenza A/Puerto Rico/8/34 (H1N1; PR8) virus revealed a significant increase in their levels compared to native control. A strong correlation was observed between hydroxy fatty acid and SFAHFAs. Detection, characterization, and profiling of these new SFAHFA levels in relation with pandemic H1N1; PR8 influenza virus will contribute to the in-depth study of their function and metabolism.

Lipidomic analysis of non-esterified furan fatty acids and fatty acid compositions in dietary shellfish and salmon by UHPLC/LTQ-Orbitrap-MS
Siddabasave Gowda B. Gowda, Yusuke Minami, Divyavani Gowda, Daisuke Furuko, Hitoshi Chiba, Shu-Ping Hui
Food Research International, 144, 110325, 110325, Elsevier BV, 2021年03月, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Lipids such as furan fatty acids (F-acids) are the valuable minor bioactive components of food such as fatty fish and plants. They are reported to have positive health benefits, including antioxidant and anti-inflammatory activities. Despite their importance, limited studies are focusing on F-acid determination in dietary seafood. This study aimed to identify and profile non-esterified F-acids and free fatty acids in total lipid extract of seafood such as shellfish and salmon. The lipidomic analysis using liquid chromatography-linear trap quadrupole-orbitrap mass spectrometry led to identifying seven types of free F-acids in shellfish (n = 5) and salmon (n = 4). The identified F-acids were confirmed by their high-resolution masses and acquired mass spectra. The relative concentrations of F-acids in shellfish range from 0.01 to 10.93 mg/100 g of the fillet, and in salmon, 0.01 to 14.21 mg/100 g of the fillet. The results revealed the highest abundance of F-acids in Sakhalin surf clam, Japanese scallop, and a fatty salmon trout. Besides, relative levels of saturated, monounsaturated, and polyunsaturated fatty acids (PUFAs) in these seafoods were compared with each other, suggesting basket clams and salmon trout to have significantly higher levels of PUFAs. The dietary seafoods enriched with F-acids and PUFAs may have possible health benefits. Hence, the applied technique could be a promising tool for rapid detection and analysis of non-esterified fatty acids in food.

LC-MSによる非アルコール性脂肪性肝疾患患者の血清リゾホスファチジルエタノールアミンレベルの分析
Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Takayuki Furukawa, Siddabasave Gowda, B. Gowda, Yue Wu, Kazuhiro Nouso, Yuki Fujii, Yuki Yoshikawa, Hitoshi Chiba, Shu-Ping Hui
Analytical and Bioanalytical Chemistry, 413, 1, 245, 254, Springer Science and Business Media LLC, 2020年10月22日, ［査読有り］, ［責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Lysophosphatidylethanolamines (LysoPEs) are the partial hydrolysis products of phosphatidylethanolamine. Despite the unique in vitro bioactivities of LysoPEs, there are limited reports on the pathophysiological role of LysoPEs in the serum, due to the lack of sensitive analytical methods for determination of each molecular species in clinical samples. Herein, we developed a highly sensitive quantitative method to profile the serum LysoPE species by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with selected reaction monitoring (SRM). The internal standard (IS), chemically synthesized in-house, and the lineup of seven major LysoPE species were used in this study. The limits of detection and quantification for each LysoPE species ranged within 0.5-3.3 pmol/mL and 1.0-5.0 pmol/mL, respectively. The combined concentrations of LysoPEs in the serum from healthy subjects (n = 8) and the patients with non-alcoholic fatty liver diseases (NAFLD) including simple steatosis (SS, n = 9) and non-alcoholic steatohepatitis (NASH, n = 27) were 18.030 ± 3.832, 4.867 ± 1.852, and 5.497 ± 2.495 nmol/mL, respectively. The combined and individual concentrations of LysoPEs, except for LysoPE 18:0, significantly decreased in the patients with NAFLD compared with those for the healthy subjects. However, no significant difference was observed between the SS and NASH groups. Our proposed LC-MS/MS method is valid and has advantages of small sample volume, high sensitivity, and simultaneous absolute quantitation for multiple molecular species. This method may enable diagnostic evaluation and elucidation of the as-yet uncovered pathophysiological role of LysoPEs.

ヒドロキシ脂肪酸の短鎖脂肪酸エステルの化学的標識支援検出および同定
Siddabasave Gowda B. Gowda, Divyavani Gowda, Chongsheng Liang, Yonghan Li, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu-Ping Hui
Metabolites, 10, 10, 398, 398, MDPI AG, 2020年10月08日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.

Identification of short‐chain fatty acid esters of hydroxy fatty acids (SFAHFAs) in a murine model by nontargeted analysis using ultra‐high‐performance liquid chromatography/linear ion trap quadrupole‐Orbitrap mass spectrometry
Siddabasave Gowda B. Gowda, Chongsheng Liang, Divyavani Gowda, Fengjue Hou, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu‐Ping Hui
Rapid Communications in Mass Spectrometry, 34, 17, e8831, Wiley, 2020年09月15日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, RATIONALE: Fatty acid esters of hydroxy fatty acids (FAHFAs) are recently discovered endogenous lipids with outstanding health benefits. FAHFAs are known to exhibit antioxidant, antidiabetic and anti-inflammatory properties. The number of known long-chain FAHFAs in mammalian tissues and dietary resources increased recently because of the latest developments in high-resolution tandem mass spectrometry techniques. However, there are no reports on the identification of short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs). METHODS: Intestinal contents, tissues, and plasma of rats fed with high-fat diet (HFD) and normal diet (ND) were analyzed for fatty acids, hydroxy fatty acids, and FAHFAs using ultra-high-performance liquid chromatography (UHPLC) and linear trap quadrupole-Orbitrap mass spectrometry (LTQ Orbitrap MS) with negative heated electrospray ionization. RESULTS: Untargeted analysis of total lipid extracts from murine samples (male 13-week-old WKAH/HKmSlc rats) led to the identification of several new SFAHFAs of acetic acid or propanoic acid esterified long-chain (>C20)-hydroxy fatty acids. Furthermore, MS3 analysis revealed the position of the hydroxyl group in the long-chain fatty acid as C-2. The relative amounts of SFAHFAs were quantified in intestinal contents and their tissues (Cecum, small intestine, and large intestine), liver, and plasma of rats fed with HFD and ND. The large intestine showed the highest abundance of SFAHFAs with a concentration range from 0.84 to 57 pmol/mg followed by the cecum with a range of 0.66 to 28.6 pmol/mg. The SFAHFAs were significantly altered between the HFD and ND groups, with a strong decreasing tendency under HFD conditions. CONCLUSIONS: Identification of these novel SFAHFAs can contribute to a better understanding of the chemical and biological properties of individual SFAHFAs and their possible sources in the gut, which in turn helps us tackle the role of these lipids in various metabolic diseases.

Untargeted Lipidomic Analysis of Plasma from High-fat Diet-induced Obese Rats Using UHPLC–Linear Trap Quadrupole–Orbitrap MS
Siddabasave Gowda B. GOWDA, Zi-Jun GAO, Zhen CHEN, Takayuki ABE, Shota HORI, Satoru FUKIYA, Satoshi ISHIZUKA, Atsushi YOKOTA, Hitoshi CHIBA, Shu-Ping HUI
Analytical Sciences, 36, 7, 821, 828, Japan Society for Analytical Chemistry, 2020年07月10日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, High-fat diet (HFD)-induced obesity is a primary risk factor for serious health problems. Although much research has been performed at the genomic level, lipidomic studies were limited. In this study, we aim to obtain a comprehensive profile of circulating plasma lipids, which are altered in rodent rat obesity by untargeted liquid chromatography-mass spectrometry. Rats fed with HFD for 8 weeks had increased body weight, liver and adipose tissue weight. The analysis results revealed that polyunsaturated fatty acids (PUFAs) and their corresponding phosphatidylcholine, phosphatidylinositol, and phosphatidylserine were significantly decreased in rats fed with HFD. In contrast, less unsaturated and ether type phosphatidylglycerols were increased. The triacylglycerides (TAGs) having saturated FA were increased in the HFD condition, whereas TAGs having PUFA were decreased. The levels of many plasma lipids were altered, and interestingly PUFA derived lipids were negatively associated with obesity. This signifies the importance of a PUFAs enriched diet to overwhelm obesity associated diseases.

A Simple and Efficient Method for Synthesis of sn ‐Glycero ‐Phosphoethanolamine
Siddabasave Gowda B. Gowda, Hirotoshi Fuda, Yusuke Yamamoto, Hitoshi Chiba, Shu‐Ping Hui
Lipids, 55, 4, 395, 401, Wiley, 2020年07月, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, An efficient three-step strategy for the convenient synthesis of Sn-glycero-3-phosphoethanolamine (GroPEtn) from a commercially available 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) is reported. Direct hydrolysis of DPPE produces a complex inseparable mixture, hence a protection and deprotection strategy is employed to prepare GroPEtn. The primary amine of DPPE is protected with a highly stable acid-labile trityl group, followed by strong base hydrolysis of N-trityl-DPPE gives N-trityl-GroPEtn. Further a mild, rapid, and efficient deprotection method is established using trifluoroacetic acid to remove N-trityl moiety, affords GroPEtn as a single product. This is the first semisynthetic approach and efficient method to produce GroPEtn with a total yield of 66% in three steps. GroPEtn did not show any cytotoxicity against human kidney (HK-2) cells and reporter gene assay for activation of Keap1-Nrf2-mediated antioxidant defense mechanism showed no significant effects.

Discovery of Eicosapentaenoic Acid Esters of Hydroxy Fatty Acids as Potent Nrf2 Activators
Siddabasave Gowda B. Gowda, Hirotoshi Fuda, Takayuki Tsukui, Hitoshi Chiba, Shu-Ping Hui
Antioxidants, 9, 5, 397, 397, MDPI AG, 2020年05月08日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids with anti-inflammatory and anti-diabetic properties. Despite the possible link between endogenous FAHFA levels and nuclear factor erythroid 2-related factor 2 (Nrf2), their possible function as antioxidants and the mechanisms involved in this are unknown. Here, we investigate FAHFAs’ plausible antioxidant potential with reference to their effect on the Nrf2 levels, oxidative stress, and lipid droplet oxidation in human hepatocytes (C3A). Six authentic FAHFAs were chemically synthesized and performed activity-based screening by reporter gene assay. Among them, eicosapentaenoic acid (EPA) esterified 12-hydroxy stearic acid (12-HSA) and 12-hydroxy oleic acid (12-HOA) FAHFAs showed less cytotoxicity compared to their free fatty acids and potent activators of Nrf2. To define their mode of action, relative levels of nuclear Nrf2 were determined, which found a higher amount of Nrf2 in nucleus of cells treated with 12-EPAHSA compared to the control. Furthermore, 12-EPAHSA increased the expression of Nrf2-dependent antioxidant enzyme genes (NQO1, GCLM, GCLC, SOD-1, and HO-1). Fluorescence imaging analysis of linoleic-acid-induced lipid droplets (LDs) in C3A cells treated with 12-EPAHSA revealed the strong inhibition of small-size LD oxidation. These results suggest that EPA-derived FAHFAs as a new class of lipids with less cytotoxicity, and strong Nrf2 activators with plausible antioxidant effects via the induction of cytoprotective proteins against oxidative stress, induced cellular damage.

Facile Chemoselective Strategy toward Capturing Sphingoid Bases by a Unique Glutaraldehyde-Functionalized Resin.
Siddabasave Gowda B Gowda, Atsufumi Nakahashi, Keiko Yamane, Saori Nakahashi, Yuta Murai, Ananda Kumar C Siddegowda, Mostafa A S Hammam, Kenji Monde
ACS omega, 3, 1, 753, 759, 2018年01月31日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Sphingoid bases, which have a 2-amino-1,3-diol common functional group, are the structural backbone units of all sphingolipids. Recently, much attention has been focused on sphingoid bases because of their potentially beneficial bioactivities toward various cancer cells as well as their dietary interest. However, low abundance and the handling complexity caused by their amphiphilic character led to very limited research on them. Glutaraldehyde has two aldehyde groups, and it reacts rapidly with the 2-amino-1,3-diol functional group of sphingosine to give a tricyclic product. Immobilization of glutaraldehyde on a resin was successfully performed by organic synthesis, starting from trans-p-coumaric acid via eight steps. This approach suppresses the self-polymerization of glutaraldehyde, and addition of water to the developed resin causes the formation of cyclic double hemiacetal function, which avoids oxidation like a reducing sugar in nature and makes it stable even for up to 1 year incubation. The resin was applied to the solid-phase extracting experiment of free sphingosine from human serum at a concentration of 280 nM. Another extraction study of edible golden oyster mushrooms showed that the sphingoid base was selectively captured from complex natural extracts. These results demonstrate that the developed glutaraldehyde resin method is a highly selective method, and hence, the combination of it with the o-phthaldialdehyde HPLC method was confirmed as an efficient and sensitive method for analysis of sphingoid bases in biological samples.

“Facile determination of sphingolipids under alkali conditions using metal free column by LC-MS/M
ボメゴウダシッダバサヴゴウダ
Analytical Bioanalytical Chemistry, 410, 20, 4793, 4803, 2018年, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Extraction and analysis of sphingolipids from biological samples is a critical step in lipidomics, especially for minor species such as sphingoid bases and sphingosine-1-phosphate. Although several liquid chromatography-mass spectrometry methods enabling the determination of sphingolipid molecular species have been reported, they were limited in analytical sensitivity and reproducibility by causing significant peak tailing, especially by the presence of phosphate groups, and most of the extraction techniques are laborious and do not cover a broad range of sphingolipid metabolites. In this study, we developed a rapid single-phase extraction and highly sensitive analytical method for the detection and quantification of sphingolipids (including phosphates) comprehensively using liquid chromatography-triple quadruple mass spectrometry. After validating the reliability of the method, we analyzed the intestinal tissue sphingolipids of germ-free (GF) and specific pathogen-free (SPF) mice and found significantly higher levels of free sphingoid bases and sphingosine-1-phosphate in the GF condition as compared to the SPF condition. This method enables a rapid extraction and highly sensitive determination of sphingolipids comprehensively at low femtomolar ranges. Graphical abstract Diagrammatic comparision of sphingolipid (phosphates) analysis between conventional and this method.

Stereochemical Study of Sphingosine by Vibrational Circular Dichroism
Atsufumi Nakahashi, Ananda Kumar C. Siddegowda, Mostafa A. S. Hammam, Siddabasave Gowda B. Gowda, Yuta Murai, Kenji Monde
ORGANIC LETTERS, 18, 10, 2327, 2330, 2016年05月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）

Highly efficient preparation of sphingoid bases from glucosylceramides by chemoenzymatic method
Siddabasave B. Gowda Gowda, Seigo Usuki, Mostafa A. S. Hammam, Yuta Murai, Yasuyuki Igarashi, Kenji Monde
JOURNAL OF LIPID RESEARCH, 57, 2, 325, 331, 2016年02月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）

トランス脂肪酸の高感度LC/MS分析法の開発
上田里奈湖, SIDDABASAVE GOWDA B. Gowda, GOWDA Divyavani, 千葉仁志, 惠淑萍, 日本栄養・食糧学会大会講演要旨集, 78th, 2024年

スフィンゴミエリン合成酵素を標的とした肥満制御のための食品中の機能性物質の探索
PUNITH M. Sundaraswamy, SIDDABASAVE GOWDA B. Gowda, SIDDABASAVE GOWDA B. Gowda, GOWDA Divyavani, 千葉仁志, 惠淑萍, 日本栄養・食糧学会大会講演要旨集, 78th, 2024年

LC/MSを用いた食品中の新規脂質代謝物とその機能の探索
SIDDABASAVE GOWDA B. Gowda, SIDDABASAVE GOWDA B. Gowda, 柳本幸太, NATH Lipsa Rani, 南雄介, GOWDA Divyavani, 千葉仁志, 惠淑萍, 日本栄養・食糧学会大会講演要旨集, 78th, 2024年

UHPLC/LTQ Orbitrap MSを用いた日本産魚類における脂質組成の網羅的解析
MINAMI Yusuke, BOMMEGOWDA Siddabasavegowda, GOWDA Divyavani, CHIBA Hitoshi, HUI Shu-Ping, JSBMS Letters, 46, Supplement, 2021年

薬剤耐性白血病の脂質バイオマーカー発見のための脂質プロフィール
MATSUHISA Chihiro, BOMMEGOWDA Siddabasavegowda, USUKI Seigo, SAKURAI Toshihiro, CHIBA Hitoshi, HUI Shu-Ping, JSBMS Letters, 46, Supplement, 2021年

LC/MSで脂肪酸ヒドロペルオキシドのワンステップ誘導体化によるターゲット分析
LIANG Chongsheng, BOMMEGOWDA Siddabasavegowda, SAKURAI Toshihiro, CHIBA Hitoshi, HUI Shu-Ping, JSBMS Letters, 46, Supplement, 2021年

LC/MSによる早期疾患バイオマーカー探索のためのグローバル脂質プロファイリング
BOMMEGOWDA Siddabasavegowda, GOWDA Divyavani, LIANG Chongsheng, CHIBA Hitoshi, HUI Shu-Ping, JSBMS Letters, 46, Supplement, 2021年

短鎖脂肪酸エステル化ヒドロキシ脂肪酸の合成と分析方法の開発
HOU Fengjue Hou, BOMMEGOWDA Siddabasavegowda, GOWDA Divyavani, CHIBA Hitoshi, HUI Shu-Ping, JSBMS Letters, 46, Supplement, 2021年

環境・エネルギーハーベスティングのためのナノコンポジット材料の進歩　　　　　　　　　　　　　　　
カーボンナノ構造体ベースの環境・エネルギー応用複合材料
スプリンガー・ネイチャー, 2022年, 9783030943196, ［共著］

マウスおよび虚血性心疾患患者における急性期から慢性期までの脂質動態。　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, Gowda D, Chiba H, Parcha V, Arora P, Halade G, Hui S-P
第17回がん・炎症・関連疾患における生理活性脂質、2022年10月30日-11月02日、米国ニューオリンズ。
2022年10月30日 - 2022年11月02日

ヒドロキシ脂肪酸の短鎖脂肪酸エステル類の簡便な分析法　　　　　　　　　　　　　　　
Fengiue H, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
17th Bioactive lipids in Cancer, Inflammation and Related Diseases, Oct-30- Nov. 02, 2022, New Orleans, USA.
2022年10月30日 - 2022年11月02日

カソケロウィルス感染による脂質代謝への影響に関する検討　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, Kajihara M, Ohno M
The 8th Hokkaido University Cross-Departmental Symposium, 2022, Oct 28, Sapporo,
2022年10月28日 - 2022年10月28日

LC/MSによる心不全の脂質バイオマーカーの解析　　　　　　　　　　　　　　　
Siddabasave gowda, B. Gowda
第５回学術集会 / 第２回中性脂肪月間, 2022, Oct 15, 福岡、日本
2022年10月15日 - 2022年10月15日

肥満症は世界的に懸念されている生活習慣病　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda
第2回国際会議 国連世界平和協会2022、, 英語, 口頭発表（一般）
2022年10月09日 - 2022年10月11日, ［招待講演］

食用海藻5種における脂質の特性解析について 液体クロマトグラフィー/質量分析法　　　　　　　　　　　　　　　
Chen Y, Hui S-P, Chiba H, D, Gowda, Siddabasave Gowda, B. Gowda
61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
2022年09月30日 - 2022年10月02日

ヒドロキシ脂肪酸の短鎖脂肪酸エステルの液体クロマトグラフィー/質量分析による解析 クロマトグラフィー／質量分析計による分析　　　　　　　　　　　　　　　
Li Y, Siddabasave Gowda, B. Gowda, D, Gowda, Hui S-P, Chiba H
61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
2022年09月30日 - 2022年10月02日

インフルエンザウイルスA / PR8 / 34 (H1N1)のスフィンゴシン-1-リン酸シグナルに対する をターゲットとしたLC / MSによるマウスでの解析　　　　　　　　　　　　　　　
D., Gowda, Chiba H, Hui S-P, Siddabasave Gowda, B. Gowda
61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
2022年09月30日 - 2022年10月02日

"液体クロマトグラフィー質量分析法によるインフルエンザウイルス感染症の脂質バイオマーカー解析"　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
2022年09月30日 - 2022年10月02日

“肥満に関連するスフィンゴ脂質代謝に対する食品由来阻害剤の発見“.　　　　　　　　　　　　　　　
Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
61回日本臨床化学会年次学術集会”. 2022, 富山県, 日本. 2022.
2022年09月30日 - 2022年10月02日

ヒドロキシ脂肪酸の短鎖脂肪酸エステル" の簡便な分析法の開発　　　　　　　　　　　　　　　
Hou F, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
第47回日本医用マススペクトル学会年会. 2022, Sept 9-10, 東京, 日本.
2022年09月09日 - 2022年09月10日

食用魚11品目の未知脂質に挑むと同時に、その組成をアンターゲットLC/MSで解析 アンターゲットLC/MSによる組成分析 "　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, Minami Y, D., Gowda, Chiba H, Hui S-P
第47回日本医用マススペクトル学会年会. 2022, Sept 9-10, 東京, 日本., 英語, 口頭発表（一般）
2022年09月09日 - 2022年09月10日

"食品由来の肥満関連スフィンゴ脂質代謝阻害剤の発見"　　　　　　　　　　　　　　　
Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
第47回日本医用マススペクトル学会年会. 2022, Sept 9-10, 東京, 日本., ポスター発表
2022年09月09日 - 2022年09月10日

アンターゲットLC/MSによる11種類の食用魚の脂質フィンガープリント法　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, Minami Y, Gowda D, Chiba H, Hui S-P
第6回国際シンポジウム「医療と食品におけるファイトケミカル」開催, 2022年08月05日, エウェ語, 口頭発表（一般）
2022年08月13日, ［招待講演］

Exploring Functional Lipids in Food, Health, and Disease by state-of-the-art of mass spectrometry　　　　　　　　　　　　　　　
Siddabasave gowda, Bomme Gowda
Two days Virtual International Conference on Advanced Materials, Dept. of Organic Chemistry, University of Mysore, India., 2022年03月04日, 英語, 口頭発表（招待・特別）

急性心筋梗塞から慢性心筋梗塞までの脂質のキネティックプロファイル　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, Gowda D, Chiba H, Hui S-P
第61回日本臨床化学会年次学術集会.(online) 2021,東京, 日本., 2021年11月06日, 口頭発表（一般）

健康・疾病における機能性脂質の化学　　　　　　　　　　　　　　　
Siddabasave gowda, Bomme Gowda
A Virtual Conference on Recent Advances in Chemical Science and Medicinal Chemistry,JSS Science & Technology University, India, 2021年07月07日, 英語, 口頭発表（招待・特別）
［招待講演］

LC-MS/MSを用いたワンステップ誘導体化による脂質ヒドロペルオキシドの測定　　　　　　　　　　　　　　　
Chongsheng Liang, Siddabasavegowda, B. Gowda, Hitoshi Chiba, Shu-ping Hui
第60回日本臨床化学会年次学術集会, ポスター発表
2020年10月26日 - 2020年11月01日

心筋梗塞におけるS1Pシグナリングの役割　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, Divayvani Gowda, Hitoshi Chiba, Ganesh Halade, Shu-ping Hui
第60回日本臨床化学会年次学術集会, ポスター発表
2020年10月26日 - 2020年11月01日

"Nrf2を介した強力な抗酸化物質としてのn-3系脂肪酸由来の脂質の発見"　　　　　　　　　　　　　　　
Hitoshi Chiba, Siddabasavegowda, B. Gowda, Hirotoshi Fuda, Takayuki Tsukui, Shu-Ping Hui
第60回日本臨床化学会年次学術集会, 口頭発表（一般）
2020年10月26日 - 2020年11月01日

Global lipid fingerprinting for biomarker discovery and their functional studies　　　　　　　　　　　　　　　
Siddabasave gowda, B. Gowda
第6回 北大・部局横断シンポジウム, 口頭発表（一般）
2020年10月19日 - 2020年10月19日

リピドミクスによる健 康と病気における脂質 の役割の解明　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda
超異分野meetup- 2020 Sapporo, Japan (札幌，日本), ポスター発表
2020年03月03日 - 2020年03月04日

Improved method for analysis of sphingolipids by ultra-high performance liquid chromatography- mass spectrometry”2nd Japan-Korea Lipid symposium　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
2nd Japan-Korea Lipid symposium, 2018年09月12日, 英語, 口頭発表（一般）
［国際会議］

Application of novel polymer sustained glutaraldehyde resin towards rapid extraction of sphigolipids　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
Japan-Taiwan Bilateral polymer symposium, 2015年09月02日, 英語, ポスター発表
［国際会議］

A novel approach towards efficient supply of natural sphingoid bases and their application as multi drug resistance inhibitors in cancer　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
14th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases, 2015年07月12日, 英語, ポスター発表
［国際会議］

Development of novel extraction method of natural sphingoids by chemoselective capturing reaction　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
27th Banyu Sapporo Symposium, 2015年07月04日, 英語, ポスター発表
［国内会議］

Application of novel polymer sustained glutaraldehyde resin towards rapid extraction of sphingoids　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
64th SPSJ Annual Meeting, 2015年05月27日, 英語, ポスター発表
［国内会議］

Innovative tactic towards rapid isolation of unique bioactive sphingoids from dietary natural resources　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
249th ACS National Meeting & Exposition, 2015年03月22日, 英語, 口頭発表（一般）
［国際会議］

Development of a highly efficient novel methodology for the rapid isolation of bioactive sphingoid base – toward construction of their chemical library　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
The 2nd International Life-Science Symposium for Young Scientists, 2014年10月23日, 英語, 口頭発表（一般）
［国際会議］

Development of a highly efficient novel methodology for the isolation of sphingoid bases and its application towards construction of their chemical library　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
The 12th International Symposium for future Drug Discovery and Medical Care, 2014年09月05日, 英語, ポスター発表
［国際会議］

Chemoselective capture of sphingolipids by efficient resin and stereochemical analysis method　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
Molecular Chirality 2014, 2014年06月06日, 英語, ポスター発表
［国内会議］

A new tactic towards rapid extraction of unique sphingoid bases　　　　　　　　　　　　　　　
ボメゴウダシッダバサヴゴウダ
Chemical Society Japan Meeting, 2014年01月27日, 英語, 口頭発表（一般）
［国内会議］

ヒドロキシ脂肪酸の短鎖脂肪酸エステル - 肥満とインフルエンザ感染の新しい腸内脂質バイオマーカー　　　　　　　　　　　　　　　
SIDDABASAVEGOWDA B. GOWDA, CHONGSHENG LIANG, DIVYAVANI GOWDA, FENGJUE HOU, HITOSHI CHIBU, SHU-PING HUI
口頭発表（一般）

化学的標識による短鎖脂肪酸エステルの検出と同定の支援 ラット結腸および盲腸内容物中のヒドロキシ脂肪酸の脂肪酸エステルの検出および同定の補助　　　　　　　　　　　　　　　
i Y, D, Gowda, Siddabasave Gowda, B. Gowda, Liang C, Chiba H, Hui S-P
第61回日本臨床化学会年次学術集会". 2021, 福岡、日本.

LC/MSによる早期疾患バイオマーカー探索のためのグローバル脂質フィンガープリンティング　　　　　　　　　　　　　　　
第46回日本医用マススペクトル学会年会. 2021,宮城, 日本., 口頭発表（招待・特別）

白血病における薬剤耐性の脂質バイオマーカー探索のための脂質フィンガープリンティング　　　　　　　　　　　　　　　
Matsuhisa C, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S.P
第46回日本医用マススペクトル学会年会. 2021,宮城, 日本., 英語, 口頭発表（一般）

UHPLC/LTQ Orbitrap MSを用いた食餌性日本産魚類の脂質組成の網羅的解析　　　　　　　　　　　　　　　
Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
第46回日本医用マススペクトル学会年会. 2021,宮城, 日本., 英語, 口頭発表（一般）

ヒドロキシ脂肪酸の短鎖脂肪酸エステルの合成と簡便な分析法の開発　　　　　　　　　　　　　　　
Fengiue H, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P
第46回日本医用マススペクトル学会年会. 2021,宮城,日本., 英語, 口頭発表（一般）

LC/MSを用いた脂肪酸ヒドロペルオキシドのワンステップ誘導体化によるターゲット分析　　　　　　　　　　　　　　　
Liang C, Siddabasave Gowda B, Sakurai T, Chiba H, Hui S.P
第46回日本医用マススペクトル学会年会. 2021,宮城, 日本, 英語, 口頭発表（一般）

インフルエンザウイルス感染の新規バイオマーカーとしてのヒドロキシ脂肪酸の飽和脂肪酸エステル　　　　　　　　　　　　　　　
Siddabasave Gowda, B. Gowda, D., Gowda, Oono M, Liang C, Chiba H, Hui S-P
第55回 日本臨床検査医学会北海道支部総会/第31回 日本臨床化学会北海道支部例会. 2021,北海道, 日本., 英語, 口頭発表（一般）

(肥満の新しいバイオマー カーとしての腸管内容物の ヒドロキシ脂肪酸の短鎖脂 肪酸エステルの発見　　　　　　　　　　　　　　　
第54回日本臨床検査医 学会・第30回日本臨床 化学会北海道支部例会 (誌上開催)(札幌，日 本)

非標的リピドミクス分析 による心筋梗塞の新しい脂 質バイオマーカー　　　　　　　　　　　　　　　
Siddabasave gowda, B. Gowda
第54回日本臨床検査医 学会・第30回日本臨床 化学会北海道支部例会 (誌上開催)(札幌，日 本)

B4生アシスタント講師　　　　　　　　　　　　　　　
北海道大学 大学院保健科学研究院
2022年04月 - 現在

健康科学　　　　　　　　　　　　　　　
北海道大学 大学院保健科学研究院
2021年04月 - 現在

医療英語　　　　　　　　　　　　　　　
北海道大学 大学院保健科学研究院
2021年04月 - 現在

食資源と健康　　　　　　　　　　　　　　　
Graduate School Global Food Resources
2021年04月 - 現在

グローバルな食料資源における生産　　　　　　　　　　　　　　　
Graduate School of Global Food Resources Hokkaido University
2021年04月 - 現在

フレッシュマンセミナー　　　　　　　　　　　　　　　
北海道大学 大学院保健科学研究院
2020年11月 - 現在

代謝研究のための分析化学　　　　　　　　　　　　　　　
北海道大学 大学院保健科学研究院
2020年11月 - 現在

Basic concepts in organic chemistry　　　　　　　　　　　　　　　
Faculty of Health Sciences
2020年07月 - 2020年07月

Importance of lipid extraction, analysis and biological applications　　　　　　　　　　　　　　　
Faculty of Health Sciences
2020年07月 - 2020年07月

Basic Concepts in Organic Chemistry　　　　　　　　　　　　　　　
Graduate School of Life Science, Hokkaido University

Chemistry for IIT-JEE　　　　　　　　　　　　　　　
Akaash institute, Nimbus tutorials, Yukthi Learning center

General Chemistry　　　　　　　　　　　　　　　
University of Mysore

2025年05月 - 現在
日本油化学会　　　　　　　　　　　　　　　

2025年01月 - 現在
日本脂質生化学会　　　　　　　　　　　　　　　

2025年01月 - 現在
アメリカ石油化学　　　　　　　　　　　　　　　

2024年04月 - 現在
在日インド科学者協会　　　　　　　　　　　　　　　

2024年04月 - 現在
インド 日本科学技術教育学会 (IJSSTE)　　　　　　　　　　　　　　　

2024年01月 - 現在
インドバイオテクノロジー研究協会 (BRSI）　　　　　　　　　　　　　　　

2022年04月 - 現在
中性脂肪蓄積心筋血管症研究班について　　　　　　　　　　　　　　　

2020年04月 - 現在
日本臨床化学会　　　　　　　　　　　　　　　

2022年01月
American Society for Mass Spectrometry　　　　　　　　　　　　　　　

2021年04月
Japanese Society for Biomedical Mass Spectrometry　　　　　　　　　　　　　　　

2021年04月
American Chemical Society　　　　　　　　　　　　　　　

2021年04月
International Lipidomics Society　　　　　　　　　　　　　　　

Targeting sphingomyelin synthase inhibition for fatty liver disease control using paprika extracts
科学研究費助成事業
2025年02月28日 - 2028年03月31日
BommeGowda SiddabasaveGowda
日本学術振興会, 基盤研究(C), 北海道大学, 25K00258

若手研究
科学研究費助成事業
2021年04月 - 2025年03月
BommeGowda SiddabasaveGowda
We achieved significant progress in our research in the last year. We successfully established the sphingomylein synthase (SMS) monitoring assay method using LC/MS and validated by in-vitro and in-silico studies using a known inhibitor for SMS. Also, the manuscript was prepared and submitted to the international peer-reviewed journal. We successfully identified the compound in Hijiki responsible for SMSs nhibition by mass spectrometry and NMR techniques. The identified compound was confirmed with authentic standard. Further, we investigated the inhibition affinity of active compounds with SMS enzymes by docking studies. The summary of results including isolation characterization and SMS assay results are under preparation for a possible manuscript while further studies are in progress.
日本学術振興会, 肥満に伴うスフィンゴ糖脂質の代謝におけるヒジキの機能的役割を探る, 北海道大学, 研究代表者, 21K14812

北海道の在来魚シシャモの肥満抑制効果に関する研究　　　　　　　　　　　　　　　
2022年度次世代研究者共同研究支援制度。
2022年08月 - 2023年03月
Siddabasave gowda, Bomme Gowda
北海道大学, 北海道大学, 研究代表者, 870411A

脂質代謝に関連する肥満症を食品バイオアクチブで克服する　　　　　　　　　　　　　　　
2022年度SOUSEI若手研究者支援プログラム助成金
2022年07月 - 2023年03月
Siddabasave gowda, Bomme Gowda
北海道大学, 北海道大学, 研究代表者, 87051V

インフルエンザ感染における新規腸管バイオマーカーの役割の検討　　　　　　　　　　　　　　　
The 7th Hokkaido University Cross-Departmental Symposium Research Grant
2021年11月 - 2022年03月
Siddabasave gowda, Bomme Gowda
北海道大学, Hokkaido University, 研究代表者

"Unrevealing the links between Kombu and obesity associated to sphingolipid metabolism"　　　　　　　　　　　　　　　
FY2021 Support System for the Collaborative Research of Next-Generation Researchers:
2021年07月 - 2022年02月
Hokkaido University, 研究代表者

Start-up grant of 300,000 yen　　　　　　　　　　　　　　　
2019年06月 - 2021年05月
ボメゴウダシッダバサヴゴウダ
Hokkaido University, 研究代表者, 競争的資金

糖スフィンゴシン及びスフィンゴ塩基の製造方法発明者　　　　　　　　　　　　　　　
特許権, ボメゴウダシッダバサヴゴウダ
特願P2014-215478, 2014年10月22日
特許P2016-79161A
2016年05月16日

Sorghum’s bioactive compounds could improve modern diets　　　　　　　　　　　　　　　
2024年10月
Hokkaido University Press Release
https://www.global.hokudai.ac.jp/blog/sorghums-bioactive-compounds-could-improve-modern-diets/, ［インターネットメディア］

Sphingosine-1-phosphate interactions in the spleen and heart reflect extent of cardiac repair in mice and failing human hearts　　　　　　　　　　　　　　　
本人以外
for details access this link: https://physiology.altmetric.com/details/112226626/news
［新聞・雑誌］