Tosa Noriko
| Faculty of Medicine Institute for Animal Experimentation | Assistant Professor |
Last Updated :2025/06/07
■Researcher basic information
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J-Global ID
Educational Organization
- Bachelor's degree program, Departments of Medicine, School of Medicine
- Master's degree program, Graduate School of Medicine
- Doctoral (PhD) degree program, Graduate School of Medicine
■Career
Career
■Research activity information
Awards
- Sep. 2023, Asian Federation of Laboratory Animal Science Assosiations, The 9th AFLAS Congress 2023 Travel Award
Analysis of storage and transport conditions of multiplex ICG sticks for diagnosis of infectious diseases in rats and mice
Noriko Tosa - May 2013, 日本実験動物技術者協会, 第4回実験動物福祉奨励賞
マウスの被毛異常または床敷湿潤が発生した後の環境エンリッチメントの効果
土佐 紀子
Papers
- Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay.
Tosa N, Ishida T, Yoshimatsu K, Hayashimoto N, Shiokawa K, Takakura A, and Arikawa J
Exp. Anim., 70, 2, 161, 168, May 2021, [Peer-reviewed], [Lead author]
English, Scientific journal - Multiplex Immunochromatographic Assay for Serologic Diagnosis of Major Infectious Diseases in Laboratory Mice.
Noriko Tosa, Tomoko Ishida, Kumiko Yoshimatsu, Nobuhito Hayashimoto, Kanae Shiokawa, Akira Takakura, Jiro Arikawa
Journal of the American Association for Laboratory Animal Science : JAALAS, 58, 6, 790, 795, 01 Nov. 2019, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal, Serologic monitoring of infectious diseases is important for microbial control in colonies of laboratory mice. Rapid and simple tests that do not require killing animals are valuable for this purpose. In this study, we developed a multiplex immunochromatographic assay (ICA) for detection of antibodies to mouse hepatitis virus (MHV), Sendai virus (also known as hemagglutinating virus of Japan [HVJ]), and Clostridium piliforme (The pathogen that causes Tyzzer disease), which are major infectious diseases in mice. For this assay, an ICA strip was put into a microtube containing 150 μL PBS and either 0.75 μL mouse serum or 1.5 μL whole blood. Binding antibodies were visualized by using protein A-conjugated colloidal gold. Under these conditions, multiplex ICA simultaneously and specifically detected antibodies to multiple antigens. To evaluate the sensitivity and specificity of multiplex ICA, positive serum samples for each infectious disease were used. Sensitivities of the multiplex ICA test for MHV, HVJ, and C. piliforme were 100%, 100%, and 90%, respectively. No nonspecific reaction was observed in any of the 30 positive sera. In addition, 10 samples of uninfected sera did not show any bands except for the control line. These observations indicate high specificity of the multiplex ICA test. Moreover, the multiplex ICA could be applied to diluted blood. These results indicate that the multiplex ICA is appropriate for rapid, simple, and safe serologic testing of laboratory mice. - Comparison of immune response in mice sensitized to an animal allergen, Can f 1, and to a food allergen, ovalbumin
Noriko Tosa, Kumiko Yoshimatsu, Motoko Takahashi, Jiro Arikawa
Biomedical Research, 40, 1, 9, 15, 2019, [Peer-reviewed], [Lead author], [Domestic magazines]
English, Scientific journal, Can f 1 belongs to the lipocalin superfamily and is considered to be an animal allergen. The immune response induced by Can f 1 in mice was compared with that induced by ovalbumin (OVA), a typical food allergen. Female BALB/c and C57BL/6 mice (6 weeks of age) were subcutaneously injected with Can f 1 or OVA with or without aluminum hydroxide (Alum) three times with intervals of two weeks. Serum levels of total IgE or antigen-specific IgE and production of IL13 and IFNγ from splenocytes were analyzed. Immunization with Can f 1 or OVA increased serum levels of both total IgE and antigen-specific IgE significantly irrespective of Alum. These results indicate that Can f 1 and OVA were able to induce allergic sensitization in mice. Splenocyte production of IL13 in mice immunized with Can f 1 or OVA with and without Alum were significantly increased after stimulation with each antigen. However, IL13 levels in the mice immunized with Can f 1 with Alum were significantly lower than those immunized without Alum. Increases in IFNγ levels after stimulation with Can f 1 or OVA were not remarkable. No influence of genetic backgrounds of BALB/c and C57BL/6 mice was found. Although Can f 1 induced Th2 type immune responses as was also the case for immunization with OVA, an inhibitory effect of Alum on induction of IL13 was observed only in mice immunized with Can f 1. These results suggest that the immune mechanism for allergic sensitization with Can f 1 is different from that with OVA. - Variation in the bacterial conditions inside cages is correlated with intracage humidity and ammonia levels.
Tosa N, Yoshimatsu K, Tadasuke Tsukiyama, Hatakeyama S, Arikawa J
Lab Animal and Environ, 21, 2, 87, 98, 2013, [Peer-reviewed], [Lead author]
English, Scientific journal - Environmental enrichment after the occurrence of wet bedding and the appearance of abnormal fur in mice is effective for solving those problems
TOSA Noriko, YOSHIMATSU Kumiko, ARIKAWA Jiro
Lab Animal and Environ, 20, 1, 1, 8, 2012, [Peer-reviewed], [Lead author]
English, Scientific journal - Effect of environmental enrichment after the occurrence of wet bedding created by mice and abnormal fur in mice.
Tosa N, Yoshimatsu K, Arikawa J
J Am Assoc Lab Anim Sci, 50, 5, 779, 780, Sep. 2011, [Peer-reviewed], [Lead author] - Rab7 is a critical mediator in vesicular transport of tyrosinase-related protein 1 in melanocytes
Tokimasa Hida, Hitoshi Sohma, Yasuo Kokai, Akinori Kawakami, Kuninori Hirosaki, Masae Okura, Noriko Tosa, Toshiharu Yamashita, Kowichi Jimbow
JOURNAL OF DERMATOLOGY, 38, 5, 432, 441, May 2011, [Peer-reviewed]
English, Scientific journal - The functional role of the glucocorticoid receptor (Gr) and Nur77 in thymocyte development
Noriko Tosa, Takahiro Fukumoto, Tadaaki Miyazaki
Nuclear Receptors, 67, 86, Nova Science Publishers, Inc., Mar. 2011
English, In book - Androgen receptors have a potential role in mediating the serotonin synthesis mechanism
Takahiro Fukumoto, Noriko Tosa, Tadaaki Miyazaki
Nuclear Receptors, 113, 120, Nova Science Publishers, Inc., Mar. 2011
English, In book - Critical function of death-associated protein 3 in T cell receptor-mediated apoptosis induction
Noriko Tosa, Atsushi Iwai, Taku Tanaka, Tomoka Kumagai, Takeshi Nitta, Satoko Chiba, Masahiro Maeda, Yousuke Takahama, Toshimitsu Uede, Tadaaki Miyazaki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 395, 3, 356, 360, May 2010, [Peer-reviewed], [Lead author]
English, Scientific journal - Functional role of death-associated protein 3 (DAP3) in anoikis
T Miyazaki, M Shen, D Fujikura, N Tosa, HR Kim, S Kon, T Uede, JC Reed
JOURNAL OF BIOLOGICAL CHEMISTRY, 279, 43, 44667, 44672, Oct. 2004, [Peer-reviewed], [Lead author]
English, Scientific journal - Functional role of DAP(death associated protein)3 in apoptosis induction
Miyazaki F, Shen M, Tosa N, Fujikura D, Kon S, Uede T, Reed JC
FASEB JOURNAL, 18, 4, A42, 23 Mar. 2004, [Peer-reviewed] - Critical function of T cell death-associated gene 8 in glucocorticoid-induced thymocyte apoptosis
N Tosa, M Murakami, WY Jia, M Yokoyama, T Masunaga, C Iwabuchi, M Inobe, K Iwabuchi, T Miyazaki, K Onoe, M Iwata, T Uede
INTERNATIONAL IMMUNOLOGY, 15, 6, 741, 749, Jun. 2003, [Peer-reviewed], [Lead author]
English, Scientific journal - Defective development of NK1.1+ T-cell antigen receptor + cells in zeta-associated protein 70 null mice with an accumulation of NK1.1+ CD3- NK-like cells in the thymus.
Iwabuchi K, Iwabuchi C, Tone S, Itoh D, Tosa N, Negishi I, Ogasawara K, Uede T, Onoe K
Blood, 91, 6, 1765, 1775, 2001, [Peer-reviewed]
English, Scientific journal - Involvement of CD28/CTLA4-B7 costimulatory pathway in the development of lymphadenopathy and splenomegaly in MRL/lpr mice
M Takiguchi, M Murakami, Nakagawa, I, MM Rashid, N Tosa, S Chikuma, A Hashimoto, T Uede
JOURNAL OF VETERINARY MEDICAL SCIENCE, 62, 1, 29, 36, Jan. 2000, [Peer-reviewed]
English, Scientific journal - Detection of various epitopes of murine osteopontin by monoclonal antibodies
H Hotta, S Kon, YU Katagiri, N Tosa, T Tsukamoto, AF Chambers, T Uede
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 257, 1, 6, 11, Apr. 1999, [Peer-reviewed]
English, Scientific journal - Haptoglobin in Carnivora: a unique molecular structure in bear, cat and dog haptoglobins.
K Mominoki, N Nakagawa-Tosa, M Morimatsu, B Syuto, M Saito
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 110, 4, 785, 9, Apr. 1995, [International Magazine]
English, Scientific journal, Haptoglobin (Hp), a hemoglobin-binding protein in plasma, consists of alpha and beta subunits and has a tetra-chain arrangement (beta-alpha-alpha-beta) connected by disulfide bridges in most mammals so far examined. Dog Hp has been reported to be unique compared with other Hps in respect that (1) the two alpha beta units are joined by a non-covalent interaction rather than a disulfide bridge and (2) the alpha chain has an oligosaccharide-binding sequence (Asn-X-Ser/Thr) and is glycosylated. To determine whether the unique structures of dog Hp are common in the Carnivora, we purified Hps from sera of bear and cat, and analyzed their subunit structure and partial amino acid sequences. The analyses by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, under both reducing and non-reducing conditions, revealed that bear and cat Hps have similar subunit arrangements to dog Hp, suggesting the absence of a disulfide bridge between two alpha chains. This was confirmed by amino acid sequence analysis of the alpha chains: that is, Cys15 participating in the inter-alpha chain disulfide bridge was replaced by Val in bear or Leu in cat and dog. Thus, the unique subunit arrangement of Hp reported in dog may be common in the Carnivora. In contrast to dog Hp, however, alpha chains of bear and cat Hps were found not to have the typical oligosaccharide binding sequence on their alpha chains and were not glycosylated. - HAPTOGLOBIN IN CARNIVORA - A UNIQUE MOLECULAR-STRUCTURE IN BEAR, CAT AND DOG HAPTOGLOBINS
K MOMINOKI, N NAKAGAWATOSA, M MORIMATSU, B SYUTO, M SAITO
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 110, 4, 785, 789, Apr. 1995, [Peer-reviewed]
English, Scientific journal - STIMULATION OF HAPTOGLOBIN SYNTHESIS BY INTERLEUKIN-6 AND TUMOR-NECROSIS-FACTOR, BUT NOT BY INTERLEUKIN-1, IN BOVINE PRIMARY CULTURED-HEPATOCYTES
N NAKAGAWATOSA, M MORIMATSU, M KAWASAKI, H NAKATSUJI, B SYUTO, M SAITO
JOURNAL OF VETERINARY MEDICAL SCIENCE, 57, 2, 219, 223, Apr. 1995, [Peer-reviewed], [Lead author]
English, Scientific journal - ISOLATION AND PRIMARY CULTURE OF BOVINE HEPATOCYTES - ALBUMIN SYNTHESIS AND ADRENERGIC ACTIVATION OF GLYCOGENOLYSIS
N NAKAGAWATOSA, M MORIMATSU, K MOMINOKI, H NAKATSUJI, B SYUTO, M SAITO
JOURNAL OF VETERINARY MEDICAL SCIENCE, 56, 1, 125, 129, Feb. 1994, [Peer-reviewed], [Lead author]
English, Scientific journal - PURIFICATION AND IDENTIFICATION OF A SERUM-PROTEIN INCREASED BY ANTHELMINTIC DRUGS FOR DIROFILARIA-IMMITIS IN DOGS
N TOSA, M MORIMATSU, M NAKAGAWA, F MIYOSHI, E UCHIDA, M NIIYAMA, B SYUTO, M SAITO
JOURNAL OF VETERINARY MEDICAL SCIENCE, 55, 1, 27, 31, Feb. 1993, [Peer-reviewed], [Lead author]
English, Scientific journal
Other Activities and Achievements
- コモンマーモセットの飼育管理における試行錯誤 ～個性豊かなマーモセットたちと向き合って～
土佐紀子, 日本実験動物技術者協会北海道支部会誌, 45, Apr. 2022, [Invited], [Lead author] - コモンマーモセット飼育室の稼働 ～沢山の方たちに助けて頂いて～
土佐紀子, 日本実験動物技術者協会北海道支部会誌, 44, 6, 10, Apr. 2021, [Invited], [Lead author]
Japanese, Others - プロポロリスコーティング床敷はケージ内常在菌の増殖とマウスアレルゲンの発生を抑制する
TOSA NORIKO, YOSHIMATSU, KUMIKO, TSUKIYAMA TADAYUI, HATAKEYAMA SHIGETSUGU, ARIKAWA JIRO, 日本実験動物学会総会講演要旨集, 61st, 280, 01 May 2014
Japanese - DAP3(death associated protein 3)を介するアポトーシスシグナルの解明
宮崎忠昭, SHEN M, 藤倉大介, 土佐紀子, 今重之, 上出利光, REED J, 日本分子生物学会年会プログラム・講演要旨集, 27th, 2004 - Functional role of DAP3 (death associated protein 3) in anoikis
T Miyazaki, M Shen, N Tosa, S Kon, T Uede, JC Reed, FASEB JOURNAL, 17, 7, C316, C316, Apr. 2003
English, Summary international conference - アポトーシス誘導におけるDAP3の機能
宮崎忠昭, CHEN M, 土佐紀子, 今重之, 上出利光, REED J, 日本分子生物学会年会プログラム・講演要旨集, 26th, 2003 - The functional analysis of DIG1 molecule, a putative G protein-coupled receptor, in dexamethasone-induced thymocyte apoptosis.
N Tosa, M Murakami, M Iwata, WY Jia, M Yokoyama, K Iwabuchi, M Inobe, T Uede, FASEB JOURNAL, 15, 4, A315, A315, Mar. 2001
English, Summary international conference - 胸腺内NK-T細胞の分化経路
岩渕和也, 岩渕千雅子, 刀祢さおり, 土佐紀子, 上出利光, 小野江和則, 日本呼吸器外科学会雑誌, 15, (), 1, 2001 - Acute Phase Response of Haptoglobin in Cattle Assessed by a Single Radial Immunodiffusion Method.
MORIMATSU MASAMI, TOSA NORIKO, NAIKI MASAHARU, SAITO MASAYUKI, 家畜生化学研究会報, 29, 61, 68, Sep. 1992
Japanese
Research Themes
- Development and Evaluation of Damage Control Resuscitation Training Curriculum for General Surgeons
Grants-in-Aid for Scientific Research
01 Apr. 2022 - 31 Mar. 2027
村上 壮一, 土佐 紀子, 方波見 謙一, 倉島 庸, 平野 聡, 本間 宙, 七戸 俊明, 伊澤 祥光
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 22K10408 - Development of rapid and simple individeual diagnostic method for major infectious diseases in laboratory animals : a multiplex immunochromatographic test
Grants-in-Aid for Scientific Research
01 Apr. 2020 - 31 Mar. 2023
土佐 紀子, 林元 展人, 石田 智子, 森松 組子
学術・科学技術の研究に供する動物の微生物モニタリングは、研究結果の信頼性の担保、および動物福祉の観点から必要不可欠である。近年、多系統少数個体維持の増加や個別換気型飼育機の普及等により、従来の微生物モニタリング方法、すなわち「おとり動物」の感染症の検出を指標とする間接的な方法では感染症を摘発することが困難となっている。この問題を解決するためには、動物を安楽死させることなく微量全血を用い、マウス、ラットの主要感染症を同時に且つ迅速・簡便に検出できる個体別血清診断法の確立が必要である。その方法として有望と考えられる「多項目イムノクロマト(ICG)法」の開発について申請者らは取り組んで来た。
本研究ではこれまでの成果を基に、１）多項目ICG法の検出条件が確定したマウス・ラットの感染症（マウス：マウス肝炎ウイルス感染症、センダイウイルス感染症、ティザー病。ラット：唾液腺涙腺炎ウイルス感染症、センダイウイルス感染症、腎症候性出血熱）の検出における本法の実用性を検証し、２）上記感染症に加え、主要感染症であるマイコプラズマ感染症（マウス、ラット）とティザー病（ラット）のICG法を確立することを目的としている。２年度（令和３年度）においては、以下の研究を実施した。
１．多項目ICGスティックの最適な保管条件・最長保管期間を明らかにする実験において、保管期間６ヶ月および１２ヶ月の多項目ICGスティックの各保管条件における検出・感度特異性への影響の結果を得た。
２．乾燥血液を用いた時のICG法の検出・感度特異性を明らかにするため、乾燥血液を作製するための条件を検討した。
３．多項目ICGスティックの輸送条件を確定するための実験設備の準備を行った。
４．Myco（マウス、ラット）とTyzzer（ラット）のICG法を確立するため、抗原の準備を行い、ICGメンブレンに塗布する抗原処理方法の実験に着手した。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20K06456 - Development of new DCS training course aiming at improving the survival rate of severe trauma patients in non-urban area
Grants-in-Aid for Scientific Research
01 Apr. 2016 - 31 Mar. 2020
MURAKAMI SOICHI
We contacted 117 trauma care specialists nationwide to cooperate in selecting trauma treatment skills that general surgeons should acquire, conducted a questionnaire survey using the Delphi method, and selected 35 items with Cronbach's alpha 0.94. We also conducted the survey for the experience and proficiency of the items on this list with 739 general surgeons in Hokkaido and analyzed 444 effective responses. Eight items, splenectomy, diaphragm injury repair, intraperitoneal gauze packing, liver injury gauze packing, suture for liver injury, hepatorrhaphy for liver injury, trauma laparotomy, Focused Assessment with Sonography for Trauma (FAST) were selected as training items. Based on this result, a new training course of trauma care that is optimized for general surgeons, using simulators, living pigs, cadavers, and e-learning was established.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 16K11392 - Analysis of the relationship between T cell repertory and incidence of laboratory animal allergy by environmental factors
Grants-in-Aid for Scientific Research
01 Apr. 2015 - 31 Mar. 2018
Tosa Noriko
In this study, exposure of antigens to fetus or juvenile mice suppressed the induction of laboratory animal allergy. These results suggest incidence of laboratory animal allergy is influenced by environmental factors and the relationship between the influence and establishment of T cell repertory as T cell repertory is established in fetus or juvenile mice. Moreover, animal allergen Can f 1-specific IgE and IL13 levels in the mice immunized with Can f 1 were influenced by aluminum hydroxide, differing from the results for immunization with OVA as a food allergen. These results suggest that the immune mechanism for laboratory animal allergy is different from that with food allergy.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 15K07776 - Studies on the immunochromatography for detecting antibody to major zoonoses and infectious diseases among laboratory rat and mouse.
Grants-in-Aid for Scientific Research
01 Apr. 2015 - 31 Mar. 2018
ARIKAWA Jiro
Immunochromatography test for detecting antibody against causative agents of diseases of laboratory rat (Sendai virus, Tyzzer’s disease, Mycoplasma, sialodacryoadenitis virus, hemorrhagic fever with renal syndrome) and laboratory mouse (mouse hepatitis, Sendai virus, Tyzzer’s disease, Mycoplasma) was developed by using extracted antigen or virus particle as antigens and Protein A labeled colloidal gold particle. Results with immune serum and rat and mouse sera collected from multiple animal facilities showed the sufficient specificity and sensitivity. Further, this immunochromatography test is available with diluted blood specimens. These results indicated that this immunochromatography test is rapid and sensitive serodiagnostic tool among laboratory mouse and rats.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 15K07717 - The role of DIG-1 gene steroid-induced apoptosis.
Grants-in-Aid for Scientific Research
1998 - 2000
UEDE Toshimitsu, TOSA Noriko, IWATA Makoto
1. We cloned and sequence a DIG-1 gene that specifically expressed by thymocytes after dexamethason(DEX)treatment.
2. DIG-1 gene encodes 7 transmembrane G protein coupled receptor.
3. We have established 3 lines of DIG-1 transgenic mice.
4. In transgenic mice, thymus were significantly smaller than those in control mice. After DEX injection, the apoptotic cells within thymic cortex increased siginificantly as compared to those in control mice. The apoptotic cells were mainly CD4+CD8+ thymocytes.
5. The expression of apoptosis-related proteins, Bcl-2 and BAX were not significantly different from thosse in transgenic mice.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B)., HOKKAIDO UNIVERSITY, 10470053
