2024年03月, 日本水産学会, 2023年度日本水産学会論文賞　　　　　　　　　　　　　　　
The effect of temperature on environmental DNA degradation of Japanese eel
Kasai A;Takada S;Yamazaki A;Masuda R;Yamanaka H

2022年03月, 日本水産学会, 2021年度日本水産学会論文賞　　　　　　　　　　　　　　　
Dispersion and degradation of environmental DNA from caged fish in a marine environment
Murakami H;Yoon S;Kasai A;Minamoto T;Yamamoto S;Sakata MK;Horiuchi T;Sawada H;Kondoh M;Yamashita Y;Masuda R

2017年03月, 水産海洋学会, 水産海洋学会論文賞　　　　　　　　　　　　　　　
Relative contribution of estuarine nursery areas to the adult population of the temperate seabass Lateolabrax japonicus in the Tango Sea, as revealed by otolith Sr:Ca ratios
冨士泰期・笠井 亮秀・上野正博・山下洋

2015年03月, 水産海洋学会, 宇田賞　　　　　　　　　　　　　　　
沿岸域の物質循環と生物生産機構に関する研究
笠井 亮秀

2012年03月, 日本水産学会, 水産学進歩賞　　　　　　　　　　　　　　　
沿岸生態系における流動環境と物質循環に関する研究
笠井 亮秀

2010年, 水産海洋学会, 2010年度水産海洋学会論文賞　　　　　　　　　　　　　　　
夏季の有明海筑後川の高濁度汽水域における粒状有機物の動態
鈴木啓太・杉本亮・笠井亮秀・小路淳・中山耕至・田中克

2008年03月, 日本水産学会, 論文賞　　　　　　　　　　　　　　　
Transport and survival processes of eggs and larvae of jackmackerel Trachurus japonicus in the East China Sea
笠井 亮秀

2007年, 日本水産学会, 2007年度日本水産学会論文賞　　　　　　　　　　　　　　　
Cellulose digestion by common Japanese freshwater clam Corbicula japonica
Sakamoto K;Touhata K;Yamashita M;Kasai A;Toyohara H

2007年, 水産海洋学会, 2007年度水産海洋学会論文賞　　　　　　　　　　　　　　　
春季の有明海筑後川の高濁度汽水域における粒状有機物の動態
鈴木啓太;杉本亮;笠井亮秀;小路淳;中山耕至;田中克

2006年03月, 日本水産学会, 論文賞　　　　　　　　　　　　　　　
Food sources for the bivalve Corbicula japonica in the foremost fishing lakes estimated from stable isotope analysis
笠井 亮秀

2002年03月, 水産海洋学会, 水産海洋学会論文賞　　　　　　　　　　　　　　　
伊勢湾の流動構造と貧酸素水塊
日本国

Seasonal changes in the protist communities of Hakodate Bay, southern Hokkaido, from 2020 to 2022
Yusuke Hamao, Kyosei Morimoto, Shoko Tatamisashi, Masahide Wakita, Akihide Kasai, Kohei Matsuno
Regional Studies in Marine Science, 78, 2024年12月15日, ［査読有り］
研究論文（学術雑誌）, Hakodate Bay at the southern end of Hokkaido experiences physical and chemical environmental changes in the adjacent Tsugaru Strait. Harmful algal blooms have occurred since 2015. However, information on the response of protist community composition, photosynthetic activity to environmental variables or the mechanisms causing harmful blooms in the Hakodate Bay is lacking. We investigated the seasonal changes in the protist community using weekly sampling to reveal the relationship between the protist community and environmental variables in the Hakodate Bay. Based on cluster analysis, six groups were identified: Winter, Spring, Late Spring, Summer, Benthic, and Karenia. High Fv/Fm was seen in the Summer, Benthic and Spring groups supported by high nutrients, warm temperature and sufficient irradiance. From the Winter to Spring group, a species transition from low-temperature-adapting Thalassiosira spp. to Chaetoceros spp. was found. The Summer group comprised high salinity-tolerant species (the diatom Leptocylindrus danicus and dinoflagellate Prorocentrum spp.) because of high fluctuations in salinity due to precipitation. The other groups (Late Spring, Benthic, and Karenia) randomly filled the spaces between the three groups. The dinoflagellate Scrippsiella spp. was dominant in the Late Spring group because of accumulation by wind-driven currents. Because a strong relationship between benthic species and wind speed was exhibited, the Benthic group comprised benthic diatoms released into the water by mixing. The Karenia group was observed only from late August 2021 to early January 2022, when higher ammonium and lower light conditions (i.e., cloudy conditions) were detected. Owing to environmental conditions, diatom growth was restricted by low light intensity, whereas that of Karenia mikimotoi increased by taking advantage of the abundant nutrients. Seasonal changes in the protist community of the Hakodate Bay can generally be explained by temperature; however, in the short term (1–2 weeks), changes were also influenced by meteorological conditions (solar radiation, wind, and precipitation).

Community Science Initiatives Utilizing Environmental DNA
Michio Kondoh, Minoru Kasada, Takuzo Abe, Akihide Kasai, Akihiro Dazai, Reiji Masuda, Satoquo Seino, Shota Suzuki, Yukari Suzuki-Ohno, Akifumi S. Tanabe
Ecological Research Monographs, 83, 99, Springer Nature Singapore, 2024年06月25日
論文集(書籍)内論文

Linkage between spatiotemporal distribution of environmental DNA and phenological activity in an amphidromous fish, ayu Plecoglossus altivelis altivelis, in a river located in its northernmost distributional area
Takuma Yonago, Tatsuya Kawakami, Akihide Kasai
Journal of Fish Biology, 104, 5, 1468, 1482, Wiley, 2024年02月18日, ［査読有り］, ［最終著者］
英語, 研究論文（学術雑誌）, Abstract

Environmental DNA (eDNA) is a promising tool for the continuous monitoring of fish ecology and diversity. However, its potential for describing the phenological activity of fish has rarely been examined. This study aimed to elucidate a linkage between the spatiotemporal distribution of eDNA and the phenology of an amphidromous fish, ayu Plecoglossus altivelis altivelis, in a river in Hokkaido, Japan, which is its northernmost distributional area. A significant positive correlation between eDNA concentration and catch per unit effort of P. a. altivelis in the river confirmed the use of eDNA as a surrogate for the abundance of P. a. altivelis. eDNA of P. a. altivelis was first detected in late April on a sandy beach adjacent to the river mouth. Subsequent to its first detection at the lowest site in the river in early May, eDNA spread throughout the river, indicating the upstream migration of P. a. altivelis. Spawning activity was also represented by a rapid increase in eDNA concentration and its surge at night in the lowest reaches of the river during September and October. These results suggest that upstream migration and spawning primarily commenced when the water temperature reached 10°C and decreased below 20°C, respectively. This observation is consistent with the behavioral responses observed in P. a. altivelis populations from other regions of Japan. Consequently, this study demonstrated that eDNA distribution was closely linked to the phenological activity of P. a. altivelis and that eDNA is a powerful tool for studying the phenology of migratory fishes.

Comparison of fish species richness and community between the two river estuaries in Hakodate Bay based on a one-year survey　　　　　　　　　　　　　　　
Hyojin Ahn, Edouard Lavergne, Yoh Yamashita, Akihide Kasai
Coastal Marine Science, 47, 8, 37, 2024年, ［査読有り］, ［最終著者］
研究論文（学術雑誌）

Multidisciplinary research for sea ice in Saroma-ko Lagoon, Hokkaido, Japan 2023（2023年北海道サロマ湖における学際的海氷研究について）
Daiki NOMURA, Ryota AKINO, Matthew CORKILL, Keizo HIRANO, Akihide KASAI, Seiji KATAKURA, Yusuke KAWAGUCHI, Tatsuya KAWAKAMI, Riri KIMURA, Delphine LANNUZEL, Ryosuke MAKABE, Mirai MATSUURA, Kohei MATSUNO, Klaus MEINERS, Keizo NAGASAKI, Yuichi NOSAKA, Nana SAMORI, Shinnosuke SAKAYA, Eun Yae SON, Ryotaro SUGA, Yumi SUNAKAWA, Keigo D. TAKAHASHI, Masaharu TAKAHASHI, Yuka TAKEDA, Takenobu TOYOTA, Manami TOZAWA, Pat WONGPAN, Hiroshi YOSHIDA, Kazuhiro YOSHIDA, Masaki YOSHIMURA
Bulletin of Glaciological Research, 42, 19, 37, Japanese Society of Snow and Ice, 2024年, ［査読有り］
研究論文（学術雑誌）

Evaluation of community science monitoring with environmental DNA for marine fish species: “Fish survey project using environmental DNA ”
Yukari Suzuki‐Ohno, Akifumi S. Tanabe, Akihide Kasai, Reiji Masuda, Satoquo Seino, Akihiro Dazai, Shota Suzuki, Takuzo Abe, Michio Kondoh
Environmental DNA, Wiley, 2023年04月18日, ［査読有り］
研究論文（学術雑誌）

Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean
Tatsuya Kawakami, Aya Yamazaki, Maki Asami, Yuko Goto, Hiroki Yamanaka, Susumu Hyodo, Hiromichi Ueno, Akihide Kasai
Ecology and Evolution, 13, 3, Wiley, 2023年03月24日, ［査読有り］, ［最終著者］
研究論文（学術雑誌）

Inshore migration of Japanese eel Anguilla japonica encouraged by active horizontal swimming during the glass eel stage
Tatsuro Karaki, Kei Sakamoto, Goro Yamanaka, Shingo Kimura, Akihide Kasai
Fisheries Oceanography, Wiley, 2023年03月20日, ［査読有り］, ［最終著者］
研究論文（学術雑誌）

Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA
Tatsuya Kawakami, Aya Yamazaki, Hai Chao Jiang, Hiromichi Ueno, Akihide Kasai
Frontiers in Marine Science, 10, 2023年, ［査読有り］, ［最終著者］
研究論文（学術雑誌）, Ongoing warming and sea-ice reductions in the Arctic can seriously impact cold-water species, such as polar cod (Boreogadus saida), necessitating biomonitoring to reveal the ecological consequences. Recent methodological advancements in environmental DNA (eDNA) techniques have increased our ability to conduct ecological monitoring at various locations, including the Arctic. This study aimed to provide an overview of the distribution of polar cod across the Bering and Chukchi Seas by employing species-specific detection of eDNA. First, we successfully developed novel species-specific qPCR assay targeting the mitochondrial D-loop region, which exclusively amplifies eDNA derived from polar cod. Subsequently, polar cod eDNA was detected using the assay from the samples that we collected latitudinally across the study area during the open-water season. Polar cod eDNA was primarily detected in the surface water from the central Chukchi Sea shelf and the northernmost observation line (75°N), which was located on the shelf slope, off the Point Barrow, and in the marginal ice zone. In contrast, only trace amounts of eDNA were detected in the Bering Sea. This pattern corresponded well with the distribution of water masses classified based on environmental conditions. The detection of eDNA in surface water was clearly limited to cold (-1 to 5°C) and low salinity (25–32) water, whereas it was detected in a higher salinity range (32–34) in the middle and bottom layers. These findings are consistent with current knowledge about the distribution and habitat of the polar cod, suggesting that eDNA can be regarded as a reliable tool to replace or supplement conventional methods. Incorporating eDNA techniques into large-scale oceanographic surveys can improve the spatial and temporal resolution of fish species detection with a reasonable sampling effort and will facilitate the continuous monitoring of Arctic ecosystems.

Modeling Coastal Ecosystem Complexes
Sachihiko Itoh, Aigo Takeshige, Akihide Kasai, Shingo Kimura, Jun Hayakawa, Naoya Ohtsuchi
Reference Module in Earth Systems and Environmental Sciences, Elsevier, 2023年, ［査読有り］
論文集(書籍)内論文

Otolith isotopic characterization as a nursery habitat indicator for stone flounder Platichthys bicoloratus
Yosuke Amano, Tsuguo Otake, Hiroyuki Togashi, Toshihiro Wada, Akihide Kasai, Yoshikazu Kato, Chikage Yoshimizu, Ichiro Tayasu, Yutaka Kurita, Kotaro Shirai
Estuarine, Coastal and Shelf Science, 277, 108028, 108028, Elsevier BV, 2022年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Dispersion and degradation of environmental DNA from caged fish in a marine environment
弘章 村上, 錫鎭 尹, 亮秀 笠井, 利文 源, 哲史 山本, 雅之 坂田, 智矢 堀内, 英樹 澤田, 倫生 近藤, 洋 山下, 玲爾 益田
NIPPON SUISAN GAKKAISHI, 88, 4, 206, 206, Japanese Society of Fisheries Science, 2022年07月15日, ［査読有り］
研究論文（学術雑誌）

Atmosphere-sea ice-ocean interaction study in Saroma-ko Lagoon, Hokkaido, Japan 2021
Daiki NOMURA, Hiroki IKAWA, Yusuke KAWAGUCHI, Naoya KANNA, Tatsuya KAWAKAMI, Yuichi NOSAKA, Sachi UMEZAWA, Manami TOZAWA, Takahito HORIKAWA, Reishi SAHASHI, Taichi NOSHIRO, Ibuki KABA, Makoto OZAKI, Fumiyoshi KONDO, Keisuke ONO, Itsuka S. YABE, Eun Yae SON, Takahiro TOYODA, Sohiko KAMEYAMA, Changqing WANG, Hajime OBATA, Atsushi OOKI, Hiromichi UENO, Akihide KASAI
Bulletin of Glaciological Research, 40, 1, 17, Japanese Society of Snow and Ice, 2022年04月, ［査読有り］, ［最終著者］
英語, 研究論文（学術雑誌）

Effects of forest cover on richness of threatened fish species in Japan
Edouard Lavergne, Manabu Kume, Hyojin Ahn, Yumi Henmi, Yuki Terashima, Feng Ye, Satoshi Kameyama, Yoshiaki Kai, Kohmei Kadowaki, Shiho Kobayashi, Yoh Yamashita, Akihide Kasai
Conservation Biology, 36, 3, Wiley, 2021年12月02日, ［査読有り］, ［最終著者］
研究論文（学術雑誌）

Quantitative Estimation of the Ecosystem Services Supporting the Growth of Japanese Chum Salmon
Yuka Karasawa, Hiromichi Ueno, Ryo Tanisugi, Ryo Dobashi, Seokjin Yoon, Akihide Kasai, Masashi Kiyota
Technical Report, 17, North Pacific Anadromous Fish Commission, 2021年11月29日, ［査読有り］
研究論文（学術雑誌）

Intraspecific genetic variability and diurnal activity affect environmental DNA detection in Japanese eel
Sayaka Takahashi, Shingo Takada, Hiroki Yamanaka, Reiji Masuda, Akihide Kasai
PLOS ONE, 16, 9, e0255576, e0255576, Public Library of Science (PLoS), 2021年09月16日, ［査読有り］, ［最終著者］, ［国際誌］
研究論文（学術雑誌）, Environmental DNA (eDNA) analysis with species-specific primer/probe sets is promising as a tool to quantify fish abundance and distribution. Nevertheless, several factors could reduce the accuracy of this method. Here, we aimed to analyze whether intraspecific variability and diel activity rhythm affect eDNA detection in Japanese eels (Anguilla japonica). For this purpose, we performed tank experiments focusing on two points. First, we assessed the effects of base pair sequences with probe region polymorphism on eDNA detection. Next, we evaluated the influences of diel rhythm, activity, and individual differences in eDNA release rate on eDNA concentration. We examined the base pair sequences of the probe regions of 20 individuals and found genetic mismatches in two of them. The eDNA concentration was estimated to be much lower in these variants than it was in the other individuals. We conducted a rearing experiment on four non-variant individuals to explore the influences of diel activity and inter-individual differences in eDNA detection. Nocturnal eel activity was reflected in the eDNA detection but the inter-individual differences remained large. The observed weak positive correlations between eDNA concentration and activity suggest that eDNA emission is highly dependent on basal metabolism. The present study suggests that consideration of polymorphic sites at the probe region and diel activity rhythms should improve the accuracy and precision of abundance estimation through eDNA. Such fine-tuning is applicable not only for eels but also for other fishes to be targeted by eDNA technology., 12963194

Distribution of Japanese Eel Anguilla japonica Revealed by Environmental DNA
Akihide Kasai, Aya Yamazaki, Hyojin Ahn, Hiroki Yamanaka, Satoshi Kameyama, Reiji Masuda, Nobuyuki Azuma, Shingo Kimura, Tatsuro Karaki, Yuko Kurokawa, Yoh Yamashita
Frontiers in Ecology and Evolution, 9, Frontiers Media SA, 2021年02月25日, ［査読有り］, ［筆頭著者, 責任著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, The abundance of Japanese eel Anguilla japonica has rapidly decreased in recent decades. Following a re-evaluation of the possibility of extinction, the Japanese Ministry of the Environment and the International Union for Conservation of Nature listed the Japanese eel as an endangered species in 2013 and 2014, respectively. However, their abundance and precise distribution have never been clarified owing to their nocturnality and difficulty in their capture. In this study, the distribution of Japanese eels was investigated by monitoring for environmental DNA (eDNA), a non-invasive and efficient detection method. A total of 365 water samples were collected from 265 rivers located throughout Japan. High concentrations of eDNA of Japanese eels were detected in rivers on the Pacific side, but were low in the Sea of Japan side. In particular, very little eDNA amplification was confirmed from Hokkaido and the north of the Sea of Japan. The eDNA distribution in Japanese rivers coincides with the transport of the larvae in the ocean, as estimated by numerical simulations. Generalized linear mixed models were developed to explain the distribution of eDNA concentrations. The total nitrogen concentration emerged as an important factor in the best model. These results indicate that the distribution of Japanese eel is mostly determined by the maritime larval transport, and their survival and growth depend on the abundance of food in the river. The findings of the present study are useful for the management of populations and in the conservation of Japanese eels., 12963194

Watershed proportion of forest cover fostering the conservation of red-listed fish richness in estuaries　　　　　　　　　　　　　　　
Lavergne E, Kume M, Ahn H, Henmi Y, Terashima Y, Ye F, Kameyama S, Kai Y, Kadowaki K, Yamashita Y, Kasai A
Conservation Biology, 2021年, ［査読有り］, ［最終著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）

Traditional land use effects on nutrient export from watersheds to coastal seas　　　　　　　　　　　　　　　
Ryo Sugimoto, Akihide Kasai, Douglas R. Tait, Takahito Rihei, Takeru Hirai, Kazuyoshi Asai, Yuji Tamura, Yoh Yamashita
Nutrient Cycling in Agroecosystems, 2020年11月11日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）

Evaluation of fish biodiversity in estuaries using environmental DNA metabarcoding
Hyojin Ahn, Manabu Kume, Yuki Terashima, Feng Ye, Satoshi Kameyama, Masaki Miya, Yoh Yamashita, Akihide Kasai
PLOS ONE, 15, 10, e0231127, e0231127, Public Library of Science (PLoS), 2020年10月06日, ［査読有り］, ［最終著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）

An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols
Toshifumi Minamoto, Masaki Miya, Tetsuya Sado, Satoquo Seino, Hideyuki Doi, Michio Kondoh, Keigo Nakamura, Teruhiko Takahara, Satoshi Yamamoto, Hiroki Yamanaka, Hitoshi Araki, Wataru Iwasaki, Akihide Kasai, Reiji Masuda, Kimiko Uchii
Environmental DNA, 3, 1, 8, 13, Wiley, 2020年08月10日, ［査読有り］, ［国際誌］
研究論文（学術雑誌）

Estimating fish population abundance by integrating quantitative data on environmental DNA and hydrodynamic modelling
Keiichi Fukaya, Hiroaki Murakami, Seokjin Yoon, Kenji Minami, Yutaka Osada, Satoshi Yamamoto, Reiji Masuda, Akihide Kasai, Kazushi Miyashita, Toshifumi Minamoto, Michio Kondoh
Molecular Ecology, 30, 13, 3057, 3067, Wiley, 2020年07月20日, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）

The effect of temperature on environmental DNA degradation of Japanese eel
Akihide Kasai, Shingo Takada, Aya Yamazaki, Reiji Masuda, Hiroki Yamanaka
Fisheries Science, 86, 3, 465, 471, Springer Science and Business Media LLC, 2020年05月, ［査読有り］, ［筆頭著者］, ［国際誌］
研究論文（学術雑誌）

Winter monsoon promotes the transport of Japanese temperate bass Lateolabrax japonicus eggs and larvae toward the innermost part of Tango Bay, the Sea of Japan
Keita W. Suzuki, Taiki Fuji, Akihide Kasai, Sachihiko Itoh, Shingo Kimura, Yoh Yamashita
Fisheries Oceanography, 29, 1, 66, 83, Wiley, 2020年01月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）

Correlation of changes in seasonal distribution and catch of red sea bream Pagrus major with winter temperature in the eastern Seto Inland Sea, Japan (1972-2010)　　　　　　　　　　　　　　　
Yamamoto M, Omi H, Yasue N, Kasai A
Fisheries Oceanography, 29, 1, 9, 2020年, ［査読有り］, ［最終著者］, ［国際誌］
英語, 研究論文（学術雑誌）

Factors structuring estuarine and coastal fish communities across Japan using environmental DNA metabarcoding
Kume M, Lavergne E, Ahn H, Terashima Y, Kadowaki K, Ye F, Kameyama S, Kai Y, Henmi Y, Yamashita Y, Kasai A
Ecological Indicators, 121, 107216, 107216, Elsevier BV, 2020年, ［査読有り］, ［最終著者］, ［国際誌］
英語, 研究論文（学術雑誌）, © 2020 The Author(s) Estuarine and coastal ecosystems are important habitats for many fish species. Large-scale patterns of estuarine and coastal fish communities can be affected by geographical factors, such as water temperature and latitude, that limit or allow fish dispersal, and/or by environmental factors that select locally adapted species. Furthermore, increased human activity has also altered fish diversity by modifying estuarine/coastal environments. Here, we used environmental DNA metabarcoding to study a large-scale pattern of estuarine/coastal fish communities across 25 watersheds in the Japanese Archipelago, and we assessed the effects of geographical, environmental, and anthropogenic factors in structuring these communities. Of the 290 fish species detected, 47 species were red-listed by the Japanese Ministry of Environment, and nine were alien. Non-metric multidimensional scaling showed that latitude and water temperature affected both river-mouth and coastal-sea fish communities. Although other anthropogenic factors did not affect these communities, bank artificialisation rates influenced both. The relationship between each fish community and ocean currents was weak. The number of red-listed fish affected both fish community structures, whereas that of alien species did not. The numbers of red-listed fish in coastal points and of all fish species were negatively influenced to the human population density. These findings provide useful information that may be used to conserve estuarine and coastal ecosystems.

Quantitative estimation of the ecosystem services supporting the growth of Japanese chum salmon
Karasawa Y, Ueno H, Tanisugi R, Dobashi R, Yoon S, Kasai A, Kiyota M
Deep Sea Research Part II, 175, 104702, 104702, Elsevier BV, 2020年, ［査読有り］, ［国際誌］
研究論文（学術雑誌）

Dispersion and degradation of environmental DNA from caged fish in a marine environment
Hiroaki Murakami, Seokjin Yoon, Akihide Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Yoh Yamashita, Reiji Masuda
Fisheries Science, 85, 2, 327, 337, Springer Science and Business Media LLC, 2019年03月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）

Age determination and growth pattern of temperate seabass Lateolabrax japonicus　　　　　　　　　　　　　　　
Jiang W, Lavergne E, Kurita Y, Todate K, Kasai A, Fuji T, Yamashita Y
Fisheries Science, 85, 81, 98, 2019年, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）

Age determination and growth pattern of temperate seabass Lateolabrax japonicus in Tango Bay and Sendai Bay, Japan (vol 85, pg 81, 2019)
JIANG Wei, LAVERGNE Edouard, KURITA Yutaka, TODATE Kana, KASAI Akihide, FUJI Taiki, YAMASHITA Yoh
FISHERIES SCIENCE, 85, 1, 99, 100, 2019年01月, ［査読有り］
英語

Modeling the coastal ecosystem complex: present situation and challenges
Sachihiko Itoh, Aigo Takeshige, Akihide Kasai, Shingo Kimura
Fisheries Science, 84, 2, 293, 307, Springer Tokyo, 2018年03月01日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, To enhance numerical modeling of the coastal ecosystem complex (CEC), we reviewed the CEC and related concepts along with the current coastal ecosystem model framework in this study. We identified two model implementation paths from the initial objectives to numerical models: specific model building, and the use of existing model frameworks. As the CEC is still at the conceptual stage, both paths are possible. Four important ecological features of CEC modeling (population connectivity, habitat heterogeneity, ontogeny of organisms, and trophic interactions) were also identified. Models for population connectivity, species distributions, life histories, and food webs were categorized using these features. We found that some previously established concepts (between–habitat interactions, coastal ecosystem mosaic, and seascape nursery) overlap with the CEC concept. Several existing integrated model frameworks were reviewed, focusing on their potential to simulate CEC processes. Building specific models for the CEC at the current conceptual stage will be challenging, and modification of existing models will be needed if they are to be used for CEC modeling. Habitat function, ontogenetic development in early life stages, and recruitment variability are important factors when modifying existing models for the development of CEC models. Although model complexity should become high to reproduce observed ecoclogical processes, an intermediate level of model ccomplexity is feasible to decrease parameter uncertainty in models for fisheries management.

Upstream migration mechanisms of juvenile temperate sea bass Lateolabrax japonicus in the stratified Yura River estuary
Taiki Fuji, Akihide Kasai, Yoh Yamashita
Fisheries Science, 84, 2, 163, 172, Springer Tokyo, 2018年03月01日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Hydrographic conditions and distributions of juvenile temperate sea bass Lateolabrax japonicus were observed in early spring from 2009 to 2012 in the Yura River estuary, which is highly stratified due to its small tides and consequent seawater intrusion into the bottom layer of the river as a salt wedge. In all four studied years, the upstream expansion of their distribution coincided with the timing of the salt wedge intrusion from the lower to upper estuary, indicating that juvenile fish used salt wedge intrusions to ascend the estuary in early spring. However, juveniles sometimes remained in the nearshore area even when the salt wedge intrusion had already occurred, indicating that other triggers are also likely to be necessary. We therefore evaluated the effects of temperature on upstream migration behaviors. The relationship between the mean temperature they experienced from hatch until starting the ascent and mean age of each cohort at the upstream migration fitted with the law of effective cumulative temperature. Most cohorts ascended the river at an effective cumulative temperature of approximately 500 °C–days. This suggests that higher temperatures would lead to a shorter period prior to the upstream migration.

Partial migration of juvenile temperate seabass Lateolabrax japonicus: a versatile survival strategy
Akihide Kasai, Taiki Fuji, Keita W. Suzuki, Yoh Yamashita
Fisheries Science, 84, 2, 153, 162, Springer Tokyo, 2018年03月01日, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Partial migration describes intrapopulation variation in the migratory behavior, i.e. some individuals from a population migrate to low-salinity river areas, while others remain in coastal areas. This paper reviews the partial migration pattern of juvenile temperate seabass Lateolabrax japonicus, which is a migration pattern not commonly seen in Japan. Seabass spawn offshore, and eggs and larvae are transported to coastal areas. Some of these juveniles then ascend rivers, while others remain in coastal areas. Juveniles efficiently use physical structures in their habitat
they use tidal currents to ascend rivers in macrotidal estuaries, while they use the salt wedge in microtidal estuaries. Once juveniles ascend the river, they can feed on the abundant prey and attain more rapid growth than those remaining in coastal areas. As estuaries are highly productive areas, they play significant roles as nurseries for juveniles of various fishes. However, compared with coastal areas, the relative area of estuaries is considerably smaller and its environmental conditions are more variable. For example, nearly 40% of adult seabass in Tango Bay were estimated to use estuarine areas as a nursery, while the other 60% use coastal areas during their juvenile stage. Using both estuaries and coastal areas through partial migration during the juvenile stage is concluded to contribute to the stabilization and yield of seabass populations.

大分県国東半島宇佐地域におけるウナギ石倉漁とその生態学的役割
原田真実, 久米学, 望岡典隆, 田村勇司, 神崎東子, 橋口峻也, 笠井亮秀, 山下洋
水産学会誌, 84, 1, 45, 53, Nihon Suisan Gakkai, 2018年, ［査読有り］, ［国内誌］
日本語, 研究論文（学術雑誌）, Local people in Oita Prefecture perform a traditional fishery called the Ishi-kura fishing method in estuaries. Ishi-kura is an underwater pile of approximately 0.5 tons of stones 15-30 cm in diameter, and wild eels dwell inside the pile and are caught using nets.We examined the relationship between Japanese eel Anguilla japonica and aquatic animals caught with an Ishi-kura net which was modified for aquatic animal research, in the Iroha and Katsura Rivers, Oita Prefecture, Japan. Based on community structure analysis, the aquatic animal community in the Ishi-kura net consisting of 54 taxonomic animals was divided into two groups by distance from the river month and substrate
the upstream group mainly contained the genus Hemigrapsus and genus Batillaria, and the downstream one predominantly contained Macrobrachium nipponense and Clithon retropicta. Based on stomach content analysis, 39 out of 79 eels consumed the same aquatic animal groups found in Ishi-kura. However, eels inhabiting downstream mainly preyed on mud shrimp, and those inhabiting upstream predominantly fed on crab, suggesting that eels may not use Ishi-kura for feeding but for living space. These findings suggest that Ishi-kura nets may be useful for monitoring eels as well as aquatic animals as their potential prey items.

Relative contributions of external forcing factors to circulation and hydrographic properties in a micro-tidal bay
Seokjin Yoon, Akihide Kasai
ESTUARINE COASTAL AND SHELF SCIENCE, 198, 225, 235, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2017年11月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, The dominant external forcing factors influencing estuarine circulation differ among coastal environments. A three-dimensional regional circulation model was developed to estimate external influence indices and relative contributions of external forcing factors such as external oceanic forcing, surface heat flux, wind stress, and river discharge to circulation and hydrographic properties in Tango Bay, Japan. Model results show that in Tango Bay, where the Tsushima Warm Current passes offshore of the bay, under conditions of strong seasonal winds and river discharge, the water temperature and salinity are strongly influenced by surface heat flux and river discharge in the surface layer, respectively, while in the middle and bottom layers both are mainly controlled by open boundary conditions. The estuarine circulation is comparably influenced by all external forcing factors, the strong current, surface heat flux, wind stress, and river discharge. However, the influence degree of each forcing factor varies with temporal variations in external forcing factors as: the influence of open boundary conditions is higher in spring and early summer when the stronger current passes offshore of the bay, that of surface heat flux reflects the absolute value of surface heat flux, that of wind stress is higher in late fall and winter due to strong seasonal winds, and that of river discharge is higher in early spring due to snow-melting and summer and early fall due to flood events. (C) 2017 Elsevier Ltd. All rights reserved.

Numerical simulation on distribution of environmental DNA of Japanese jack mackerel (Trachurusjaponicus) in a semi-closed bay　　　　　　　　　　　　　　　
Seokjin Yoon, Akihide Kasai, Satoshi Yamamoto, Kenji Minami, Kazushi Miyashita, Reiji Matsuda, Michio Kondoh
한국수산과학회 양식분과 학술대회, 126, 126, 2017年05月, ［査読有り］, ［国際共著］, ［国内誌］
研究論文（学術雑誌）

Food composition for blue mussels (Mytilus edulis) in the Menai Strait, UK, based on physical and biochemical analyses　　　　　　　　　　　　　　　
Morioka H, Kasai A, Miyake Y, Kitagawa T, Kimura S
Journal of Shellfish Research, 36, 659, 668, 2017年, ［査読有り］
英語, 研究論文（学術雑誌）

Agriculture, forestry and fisheries in watershed area and regional promotion-Forests, rivers, villages and oceans in Kunisaki Peninsula and Usa area of Globally Important Agricultural Heritage Systems-
Yuji Tamura, Ryo Sugimoto, Akihide Kasai, Hisashi Yokoyama, Yoh Yamashita
NIPPON SUISAN GAKKAISHI, 83, 6, 1017, 1017, JAPANESE SOC FISHERIES SCIENCE, 2017年, ［査読有り］
日本語, 研究論文（学術雑誌）

Can we verify the importance of ecological connectivity of hills, rivers, humans and oceans?
Yoh Yamashita, Akihide Kasai, Takayuki Shiraiwa, Yoshihisa Shirayama
NIPPON SUISAN GAKKAISHI, 83, 6, 1008, 1008, JAPANESE SOC FISHERIES SCIENCE, 2017年, ［査読有り］
日本語, 研究論文（学術雑誌）

Estuarine circulation-driven entrainment of oceanic nutrients fuels coastal phytoplankton in an open coastal system in Japan
Kenta Watanabe, Akihide Kasai, Koji Fukuzaki, Masahiro Ueno, Yoh Yamashita
ESTUARINE COASTAL AND SHELF SCIENCE, 184, 126, 137, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2017年01月, ［査読有り］
英語, 研究論文（学術雑誌）, We investigated interactions among seasonal fluctuations in phytoplankton biomass, riverine nutrient flux, and the fluxes of nutrients entrained by estuarine circulation in Tango Bay, Japan, to determine the influence of freshwater inflows to an open bay on coastal phytoplankton productivity. The riverine nutrient flux was strongly regulated by river discharge. Estuarine circulation was driven by river discharge, with high fluxes of nutrients (mean nitrate + nitrite flux: 5.3 +/- 3.5 Mg [mega grams]-N day(-1)) between winter and early spring, enhanced by nutrient supply to the surface water via vertical mixing. In contrast, low-nutrient seawater was delivered to the bay between late spring and summer (1.0 0.8 Mg N, day(-1)). Seasonal fluctuations in phytoplanlcton biomass were affected by the entrained fluxes of oceanic nutrients and variation in the euphotic zone depth, and to a lesser degree by the riverine nutrient flux. Bioassays and stoichiometric analyses indicated that phytoplankton growth was limited by nitrogen and/or phosphorus. Both the entrainment of oceanic nutrients and the euphotic zone depth affected the duration and magnitude of blooms. Our findings show that, unlike semi-enclosed bays, seasonal variations in coastal phytoplankton in an open coastal system are primarily fueled by the entrainment of oceanic nutrients and are influenced by both freshwater inflow and coastal conditions (e.g. vertical mixing and wind events). (C) 2016 Elsevier Ltd. All rights reserved.

Dynamics of iron in the Chikugo River Basin: Comparison of iron with nitrogen and phosphate input to the estuary
Mitsuo Yamamoto, Dan Liu, Akihide Kasai, Kei Okubo, Masaru Tanaka
REGIONAL STUDIES IN MARINE SCIENCE, 8, 89, 98, ELSEVIER SCIENCE BV, 2016年11月, ［査読有り］
英語, 研究論文（学術雑誌）, Iron (Fe) dynamics in the Chikugo River were investigated in order to understand the relationship between the characteristics of Fe concentrations in the basin and the river estuarine ecosystem and that of the inner area of the Ariake Sea. Analysis of Fe concentration from monthly samples at 29 sites in the Chikugo River from July 2011 to May 2012 was conducted together with analyses of nitrogen (N) and phosphate (P) concentrations. Fe concentration changed slightly from the upstream to the middle basin, whereas in the estuary it increased sharply at 15 km from the river mouth and decreased again near the river mouth. This trend is different from the trends for N and P concentrations, and the Fe distribution was distinctive throughout the year. Further investigation was conducted focusing on the Fe dynamics in the estuary and the inner Ariake Sea. It was found that the trend of Fe concentration corresponds to the trend of turbidity in the estuary. The reasons of the characteristic Fe dynamics are suggested that supplied Fe is accumulated by the scavenging effect of particulate organic matter in the estuarine turbidity maximum to form floccules. As these particle materials are important for forming the ecosystem in the inner Ariake Sea, it is suggested that Fe dynamics have an influence in forming the ecosystem in the Chikugo River and the Ariake Sea. (C) 2016 Elsevier B.V. All rights reserved.

Circulation and haline structure of a microtidal bay in the Sea of Japan influenced by the winter monsoon and the Tsushima Warm Current
Sachihiko Itoh, Akihide Kasai, Aigo Takeshige, Kei Zenimoto, Shingo Kimura, Keita W. Suzuki, Yoichi Miyake, Tatsuhiro Funahashi, Yoh Yamashita, Yoshiro Watanabe
JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS, 121, 8, 6331, 6350, AMER GEOPHYSICAL UNION, 2016年08月, ［査読有り］
英語, 研究論文（学術雑誌）, Mooring and hydrographic surveys were conducted in Tango Bay, a microtidal region of freshwater influence (ROFI) in the Sea of Japan, in order to clarify the circulation pattern in the bay and its driving forces. Monthly mean velocity records at four stations revealed an inflow and outflow at the eastern and northern openings of the bay, respectively, indicating an anticyclonic circulation across the bay mouth. The circulation was significantly intensified in winter, in accordance with the prevailing NW wind component of the winter monsoon. The anticyclonic circulation at the bay mouth was connected to an estuarine circulation that was evident near the mouth of the Yura River at the bay head. Surface salinity just offshore of the river mouth was closely related to the Yura River discharge, whereas in lower layers the offshore water had a stronger influence on salinity. Prior to a seasonal increase in the Yura River discharge, summer salinity decreased markedly through the water column in Tango Bay, possibly reflecting intrusion of the Changjiang Diluted Water transported by the Tsushima Warm Current. In contrast with the traditional assumption that estuarine circulation is controlled mainly by river discharge and tidal forcing, the circulation in Tango Bay is strongly influenced by seasonal wind and the Tsushima Warm Current. The narrow shelf may be responsible for the strong influence of the Tsushima Warm Current on circulation and water exchange processes in Tango Bay.

The Importance of Estuarine Production of Large Prey for the Growth of Juvenile Temperate Seabass (Lateolabrax japonicus)
Taiki Fuji, Akihide Kasai, Masahiro Ueno, Yoh Yamashita
ESTUARIES AND COASTS, 39, 4, 1208, 1220, SPRINGER, 2016年07月, ［査読有り］
英語, 研究論文（学術雑誌）, The feeding ecology of juvenile temperate seabass (Lateolabrax japonicus) in relation to various prey environments was analyzed in the Yura River estuary from 2008 to 2012. Juveniles mainly fed on copepods and mysids, both in the Yura River estuary and in the adjacent coastal area. The dependency on the mysids increased as juveniles grew from 15 to 25 mm standard length (SL), and the main stomach contents were mysids for juveniles larger than 25 mm SL. The maximum size of food contents increased from 2 to 15 mm with the growth of juveniles from 15 to 25 mm SL. Most prey items smaller and larger than 2 mm were composed of copepods and mysids, respectively. The mean size of ingested food items increased with the size of the juveniles, while the mean number of prey items in each stomach decreased with growth after juveniles reached 22 mm SL. As a result, the stomach content index increased with the size of juveniles. These results indicate that the shift to larger-sized prey items (from copepods to mysids) is important for juveniles to obtain energy efficiently. Mysids were larger and more abundant in the estuaries than those in the coastal area. Therefore, the estuary provided better foraging conditions to juvenile temperate seabass than the coastal area.

Importance of estuarine nursery areas for the adult population of the temperate seabass Lateolabrax japonicus, as revealed by otolith Sr:Ca ratios
Taiki Fuji, Akihide Kasai, Masahiro Ueno, Yoh Yamashita
FISHERIES OCEANOGRAPHY, 25, 4, 448, 456, WILEY-BLACKWELL, 2016年07月, ［査読有り］
英語, 研究論文（学術雑誌）, Otolith Sr:Ca ratios were used to evaluate the contribution of estuarine nursery areas to the temperate seabass (Lateolabrax japonicus) population in Tango Bay, the Sea of Japan. Otolith Sr:Ca ratios of juveniles collected from freshwater, brackish and coastal areas were analysed to determine a criterion for distinguishing whether individuals used estuarine or coastal areas as nurseries. Otolith Sr:Ca ratios of adult seabass collected around Tango Bay were also analysed to detect the records of salinity that juveniles experienced and to estimate the contribution of each habitat. Sr:Ca ratios of the edges of otoliths of juveniles from freshwater areas were 2.4x10(-3) in average, which were significantly lower than those of juveniles collected from a coastal area (4.9x10(-3) in average) owing to differences in salinity conditions among these areas. Based on the threshold criterion determined by this difference, 39 out of 107 (36%) adult seabass were estimated to have used the estuary as a nursery during their juvenile stage according to their Sr:Ca chronologies. Despite the small area covered by estuarine nurseries compared with coastal waters, the Yura River estuary contributed considerably to the adult seabass population. This indicates estuaries play an important role as nurseries for seabass juveniles, and it should be noted that the estuaries are important nurseries to conserve the stocks of coastal fishes.

Isotopic trophic-step fractionation of the freshwater clam Corbicula sandai
Akihide Kasai, Daisuke Ishizaki, Takane Isoda
FISHERIES SCIENCE, 82, 3, 491, 498, SPRINGER JAPAN KK, 2016年05月, ［査読有り］
英語, 研究論文（学術雑誌）, Diet-switch experiments with three different species of microalgae were conducted to estimate diet-tissue isotopic fractionation of carbon (C) and nitrogen (N) isotopes in the freshwater clam Corbicula sandai. The delta C-13 and delta N-15 values in the soft tissues of C. sandai changed in each experiment, with little inter-individual deviations, thereby reflecting the switch in diet. Isotope values of the clam reached the asymptotic value at around 40 days after the switch. Equilibrium isotopic signatures, as well as turnovers of carbon and nitrogen in the whole soft tissues, were estimated by exponential decay models. Fractionations for C. sandai varied from 0.1 to 0.7 aEuro degrees for carbon, and from 2.1 to 3.6 aEuro degrees for nitrogen; these values fell within or were close to the range of previously accepted fractionation values (0-1 aEuro degrees for carbon and 3-4 aEuro degrees for nitrogen). The half-life values for carbon in the bivalves (12-22 days) were about twofold longer than those for nitrogen (7-9 days). The specific fractionation values estimated in this study contribute towards improving our understanding of inter-specific trophic relationships and aquatic food webs.

Key biogeochemical processes evaluated by the stable nitrogen isotopes of dissolved inorganic nitrogen in the Yodo River estuary, Japan: significance of estuarine nutrient recycling as a possible source for coastal production
Ryo Sugimoto, Akihide Kasai
BIOGEOCHEMISTRY, 128, 1-2, 1, 17, SPRINGER, 2016年03月, ［査読有り］
英語, 研究論文（学術雑誌）, Isotope analysis of dissolved inorganic nitrogen (DIN) and a mixing model along the salinity gradient were used to clarify the dominant biogeochemical processes controlling nutrient dynamics within a shallow eutrophic estuary in Japan. Although delivery of riverine DIN into the estuary is largely dominated by mixing with seawater during most months, internal biogeochemical processes of DIN assimilation by phytoplankton and DIN production by remineralization and subsequent nitrification within the estuary offer the most reasonable explanation for observed deviations in the isotopic compositions of NO3- from mixing behavior. However, the balance of each process changed over time. During phytoplankton blooming in summer, co-occurrence of NO3- assimilation and NO3- regeneration inhibited the accumulation of NO3- within the estuary. Moreover, assimilation of NH4+ as well as NO3- by phytoplankton complicates the nutrient dynamics within the estuary. However, mostly conservative or productive behavior of DIN as well as PO43- showed that recycled nutrients are significant net source within the estuary. These results suggest recycled nutrients within the estuary could have a non-negligible impact on eutrophication in Osaka Bay.

環境DNA を用いた沿岸域における魚類モニタリング
源 利文, 山本 哲史, 笠井 亮秀, 近藤 倫生
沿岸海洋研究, 53, 2, 173, 178, 日本海洋学会 沿岸海洋研究会, 2016年, ［査読有り］
日本語, 研究論文（学術雑誌）, 環境DNA を用いて水中の生物分布を明らかにする手法が近年急速に発展している．環境DNA を用いた生物分布の推定手法は，種特異的な検出系を用いた特定の種の在不在を調べる方法と，メタバーコーディングを用いた生息種の網羅的検出の手法に大別できる．本稿では，これまでに報告されている研究例をあげながら，このような手法について概説する．海洋における環境DNA 分析手法の実施例はまだ報告例が少ないが，ここでは著者らの研究チームが実施した環境DNA 定量によるマアジ（Trachurus japonicus）の相対バイオマス推定の予備的な解析結果についても報告し，海域における環境DNA 分析手法の現時点での到達点について述べる．マアジの環境DNA 分析の結果は，海域においても環境DNAを用いたバイオマス推定が可能である事を示唆している．今後，環境DNA 分析によって沿岸域における魚類の生息状況や生態の把握はいっそう進むと期待され，本手法は水産学，魚類学，生態学など様々な分野に貢献することができるだろう．

The Response of Fish Communities to Climate and Human-Induced Changes Inferred from Fishery Landings in an Enclosed Bay
Sangdeok Chung, Hirokazu Suzaki, Akihide Kasai, Hideaki Nakata
ESTUARIES AND COASTS, 38, 4, 1365, 1375, SPRINGER, 2015年07月, ［査読有り］
英語, 研究論文（学術雑誌）, Variations in the fish community structure in Omura Bay, an enclosed bay in the East China Sea, were investigated using long-term fishery landing data over the past five decades (1965-2006). We confirmed that the fish community structure in Omura Bay showed three conspicuous regimes during the study period. Anchovies dominated in the pelagic domain with abundant and various demersal fish species in the first phase (1965-1978). Demersal fish declined remarkably with the dominance of sardines in the pelagic domain during the second phase (1979-1987), and the fish community was simplified with the return of anchovies and no recovery of demersal fish in the third phase (1988-2006). These changes were significantly related with periodic large-scale climate changes and cumulative regional-scale human impacts. The replacement of sardine/anchovy caused by a climate regime shift in the North Pacific contributed to the fish community regime shifts in Omura Bay. Moreover, habitat degradation such as depletion of dissolved oxygen combined with climate-induced changes in food web systems resulted in a rapid reduction in demersal fish. Contraction of the habitat volume due to an increase in oxygen-restricted water simplified the fish community structure and eventually reduced fishery landings in Omura Bay. These findings demonstrate that long-term fish community responses to large-scale climate changes and regional-scale human impacts can be observed in a coastal enclosed bay, providing useful information for fisheries management in areas with climate and human-induced changes.

Growth and migration patterns of juvenile temperate seabass Lateolabrax japonicus in the Yura River estuary, Japan-combination of stable isotope ratio and otolith microstructure analyses
Taiki Fuji, Akihide Kasai, Masahiro Ueno, Yoh Yamashita
ENVIRONMENTAL BIOLOGY OF FISHES, 97, 11, 1221, 1232, SPRINGER, 2014年11月, ［査読有り］
英語, 研究論文（学術雑誌）, It is important to know the characteristics of migration pattern and vital rates of juveniles to understand the early life history and its effect on the population dynamics of fishes. The relationship between growth and migration pattern of juvenile temperate seabass Lateolabrax japonicus in the Yura River estuary was examined by combination of stable carbon isotope ratio (delta C-13) and otolith microstructure. Gut fullness indices were also examined to know the feeding condition of juveniles. delta C-13 values of seabass juveniles in the lower estuary and surf zone (LES) were enriched, while those in freshwater zone (FW) were depleted, consistent with delta C-13 differences in prey items. The back-calculated growth rates of juveniles in FW were significantly lower than those of juveniles that resided in the LES from 50 days to 90 days old, implying that juveniles with poor growth ascended the river while those with better growth remained in the LES. However, the growth rates of the juveniles, which resided in FW for more than 1 month, caught up with and even overtook those of juveniles in LES within 1 month after ascended the river. The higher water temperature and better feeding conditions would contribute to better growth rates of juveniles in FW than those in LES.

Influence of salt-wedge intrusion on ecological processes at lower trophic levels in the Yura Estuary, Japan
Kenta Watanabe, Akihide Kasai, Emily S. Antonio, Kentaro Suzuki, Masahiro Ueno, Yoh Yamashita
ESTUARINE COASTAL AND SHELF SCIENCE, 139, 67, 77, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2014年02月, ［査読有り］
英語, 研究論文（学術雑誌）, To examine the influence of salt-wedge intrusion on ecological processes at lower trophic levels in estuaries, we investigated seasonal variability in the nutrients, phytoplankton biomass (chlorophyll a), zooplankton density, and composition in the Yura Estuary, Japan. Phytoplankton composition was determined under two discriminative regimes: salt-wedge regime (summer) and freshwater regime (winter). Phytoplankton from two groups bloomed simultaneously under the salt-wedge regime. Freshwater and brackish phytoplankton dominated the upper freshwater layer (similar to 16,100 cells mL(-1)) while marine phytoplankton were dominant below the halocline (similar to 12,200 cells mL(-1)). Both phytoplankton groups grew using riverine nutrients. Marine phytoplankton increased closer to the tip of the salt-wedge, indicating that marine phytoplankton grew in situ in the river. The residence time of salt water increased due to the low river discharge from spring to summer, enhancing mineralization in the salt-wedge. Regenerated nutrients in the salt-wedge contribute to subsequent marine phytoplankton blooms. Copepods were more abundant in the salt-wedge (similar to 80.9 ind. L-1) than in the upper freshwater layer ( similar to 142 ind. L-1) during summer. Phytoplankton and zooplankton densities under the freshwater regime (winter; similar to 360 cells mL(-1), similar to 39.7 ind. L-1) were lower than under the salt-wedge regime (summer). The high river discharge under the freshwater regime flushes out the salt-wedge and discourages biological production in the river. Our results showed that salt-wedge intrusions change the physical conditions and nutrient dynamics, enhancing biological production in the salt-wedge estuary. (C) 2014 The Authors. Published by Elsevier Ltd. All rights reserved.

Hypoxia controlled by hydrodynamics
笠井 亮秀
Aqua-Bio Science Monographs, 7, 4, 117, 145, Terrapub, 2014年, ［査読有り］
英語, 研究論文（学術雑誌）

安定同位体比を利用した河口・沿岸域における懸濁態有機物の挙動と物質循環解析 (シンポジウム 沿岸海域における懸濁物質・海底表層堆積物の動態研究の現在)
杉本 亮, 笠井 亮秀, 佐藤 専寿
沿岸海洋研究 = Bulletin on coastal oceanography, 51, 1, 19, 28, 日本海洋学会沿岸海洋研究部会, 2013年08月, ［査読有り］
日本語

Trophic characteristics of a mangrove fish community in Southwest Thailand: Important mangrove contribution and intraspecies feeding variability
Matiss Zagars, Kou Ikejima, Akihide Kasai, Nobuaki Arai, Prasert Tongnunui
ESTUARINE COASTAL AND SHELF SCIENCE, 119, 145, 152, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2013年03月, ［査読有り］
英語, 研究論文（学術雑誌）, Mangrove production has been found to make a major contribution to the nutrition of a fish community in the Sikao Creek mangrove estuary, Southwest Thailand. Gut content analysis and carbon and nitrogen stable isotope analysis were used to assess fish feeding behavior and trophic reliance on different primary producers (mangrove leaves, phytoplankton, microphytobenthos) focusing on 19 dominant fish species, and 4 potential fish food items. Cluster analysis identified 5 trophic groups and the IsoSource model indicated the importance of primary food sources in trophically supporting different fish species. Most analyzed fish species had carbon isotopic signatures that were more depleted than those reported in previous studies, and the IsoSource model indicated that mangrove leaves were an important primary food source. This may be a specific characteristic of our study site, which is not well connected to other productive coastal habitats that provide alternative primary food sources. Thus we suggest that food chains in trophically isolated mangrove estuaries of southwest Thailand are more dependent on mangrove tree production. We also assessed the relationship of individuality in fish feeding habits and variability of delta C-13 values and showed that several mangrove fish species have significant intraspecies variability in feeding habits, possibly due to high intraspecific competition. (C) 2013 Elsevier Ltd. All rights reserved.

Effects of short time variation in the river discharge on the salt wedge intrusion in the Yura Estuary, a micro tidal estuary, Japan　　　　　　　　　　　　　　　
Funahashi T, Kasai A, Ueno M, Yamashita Y
Journal of Water Resource and Protection, 5, 343-348., 2013年, ［査読有り］
英語

River ascending mechanisms of juvenile seabass　　　　　　　　　　　　　　　
Taiki Fuji, Akihide Kasai, Masahiro Ueno, Yoh Yamashita
Proceedings of the 10th Global Congress on ICM: Lessons Learned to Address New Challenges, EMECS 2013 - MEDCOAST 2013 Joint Conference, 1, 581, 590, Middle East Technical University, 2013年
英語, 研究論文（国際会議プロシーディングス）, Recent surveys have revealed that juvenile temperate seabass, Lateolabrax japonicus, migrate into upper estuaries in early spring. However, their mechanisms for river ascent are insufficiently understood. In order to clarify these mechanisms, hydrographic conditions and juvenile distribution were observed in early spring from 2009 to 2012 in the Yura River estuary, which is highly stratified due to its small tidal variations and consequent seawater intrusion into the bottom layer of the river as a salt wedge. The timing and distance of salt-wedge intrusion are mainly controlled by river discharge. In all four studied years, juveniles first ascended the river to the middle estuary 4-6 km upstream from the river mouth. Subsequently, they reached the upper estuary, 11-15 km from the river mouth, within one month. The expansion of their distribution coincided with the timing of salt-wedge intrusion from the mid- to upper estuary. This indicates that the distribution of juveniles is dependent on the extent of the seawater intrusion into the bottom layer in early spring. However, juveniles sometimes remained in the nearshore area even when the salt-wedge intrusion had already occurred, indicating that salt-wedge intrusion is only one necessary condition, and that there is likely another trigger for ascent. The effects of temperature on river ascent behaviours were evaluated. There was a significant negative relationship between the mean age of seabass juveniles at the time of river ascent and the mean temperature they experienced from birth until starting the ascent. Most juveniles ascended the river at an effective cumulative temperature of approximately 500 C. This suggests that juveniles need to reach a cumulative temperature threshold in order to start ascending the river, and higher temperatures would lead to a shorter preparation period for river ascent. It is considered that high temperatures would lead to a short period for the metamorphosis of juveniles. Metamorphosis enables seabass to adjust to environments of different salinities.

Spatial-temporal feeding dynamics of benthic communities in an estuary-marine gradient
Emily S. Antonio, Akihide Kasai, Masahiro Ueno, Yuka Ishihi, Hisashi Yokoyama, Yoh Yamashita
ESTUARINE COASTAL AND SHELF SCIENCE, 112, 86, 97, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2012年10月, ［査読有り］
英語, 研究論文（学術雑誌）, We investigated the fluctuations of carbon and nitrogen stable isotope ratios in benthic consumers and their potential food sources to determine the spatial and temporal variations in the utilization of available organic matter, indicating the origin and pathways of energy from Yura Estuary to Tango Sea, Japan. Field samplings were conducted from the upper estuary to offshore with sampling frequency of twice per season from April (spring) 2006 to February (winter) 2007. The delta C-13 signatures of the upper and lower estuary benthos showed depleted and in wide range (-28.9 parts per thousand to -13.5 parts per thousand) compared to the enriched and within narrow range signatures of marine benthos (-20.6 parts per thousand to -14.0 parts per thousand) in all seasons. On the contrary, the delta N-15 signatures of benthic communities showed decreasing trend seaward and summer values were different from the other seasons. Using the dual isotope and multisource mixing models, we estimated the relative contributions of potential food sources to the benthos diet. River POM played an important source of energy for the estuarine benthos, especially in winter when river discharge was high. Marine POM served as an important alternative food for the estuarine benthos from spring to autumn when seawater intruded the bottom estuary. Benthic microalgae were the major food source at the shallow coast throughout the year, while marine POM fueled the deep coast and offshore benthic food webs. Spatial and temporal feeding variations in estuarine benthic communities were driven by the hydrology of the estuary, whereas primary production and transport of food source dictated diet variations of marine benthic communities. The elucidation of the dynamic energy subsidy among aquatic systems highlights the importance of the land sea transition zones that is crucial for benthic secondary productions. (C) 2011 Elsevier Ltd. All rights reserved.

Year-round accumulation of particulate organic matter in the estuarine turbidity maximum: comparative observations in three macrotidal estuaries (Chikugo, Midori, and Kuma Rivers), southwestern Japan
Keita W. Suzuki, Akihide Kasai, Kouji Nakayama, Masaru Tanaka
JOURNAL OF OCEANOGRAPHY, 68, 3, 453, 471, SPRINGER, 2012年06月, ［査読有り］
英語, 研究論文（学術雑誌）, The inner part of the Ariake Sea is one of the most productive estuarine systems in Japan. To examine potential food items for estuarine organisms, we conducted monthly observations of the dynamics of particulate organic matter along the macrotidal Chikugo River estuary in 2005 and 2006. In the neighboring macrotidal Midori and Kuma River estuaries, comparative observations were made. High turbidity and strong vertical mixing were observed only at low salinities (<10) in the Chikugo River estuary. In contrast, the Midori and Kuma River estuaries were characterized by less turbid and less mixed waters. Concentrations of particulate organic carbon often exceeded 5 mg l(-1) in or close to the estuarine turbidity maximum (ETM) of the Chikugo River estuary. However, such high concentrations were rarely observed in the other two estuaries. The observed differences could be attributable to different hydrodynamic processes related to the different lengths of tidal reaches: 23, 8, and 6 km in the Chikugo, Midori, and Kuma Rivers, respectively. In the Chikugo River estuary, spatiotemporal changes of chlorophyll a suggested that phytoplankton occurred abundantly up- and/or downstream from the ETM especially during the warm season. In contrast, pheophytin (i.e., plant detritus) always accumulated in or close to the ETM. Carbon stable isotope ratios and carbon to nitrogen ratios indicated that the plant detritus was derived from phytoplankton and terrestrial plants. The Chikugo River estuary has a high potential to support the production of estuarine organisms through abundant plant detritus in the well-developed ETM all the year round.

Physical environment and material circulation in coastal ecosystems
Akihide Kasai
NIPPON SUISAN GAKKAISHI, 78, 3, 372, 375, JAPANESE SOC FISHERIES SCIENCE, 2012年05月
日本語

小型魚類の生体内元素分析を用いたダム下流域における急激な物質循環変化の検証　　　　　　　　　　　　　　　
東信行, 村元寛子, 伊藤結美, 河野冬樹, 野田香織, 笠井亮秀, 渡邉泉
河川技術論文集, 18, 405-410., 2012年, ［査読有り］
日本語

DIET SHIFT IN THE SAND SHRIMP CRANGON URITAI ALONG THE ESTUARY-MARINE GRADIENT
Emily S. Antonio, Akihide Kasai, Masahiro Ueno, Yuka Ishihi, Hisashi Yokoyama, Yoh Yamashita
JOURNAL OF CRUSTACEAN BIOLOGY, 31, 4, 635, 646, CRUSTACEAN SOC, 2011年10月, ［査読有り］
英語, 研究論文（学術雑誌）, Spatial and temporal variations in the diet of Crangon uritai were examined using stable isotope and stomach content analyses. The sand shrimp and its potential prey were collected from Yura Estuary (3-5 m), shallow coast (5-10 m), and deep coast (30-60 m) stations of Tango Sea from April 2006 to February 2007. The measurements of the length (carapace and total lengths) and feeding habit (stomach content and stable isotope ratios) of C. uritai showed that this shrimp changed its diet during migrations between the estuary and marine environments. This species consumes a variety of food at the lower estuary: mysid Orientomysis japonica, small crustaceans particularly amphipod Melita hoshinoi, and bivalve Veremolpa micra. The abundant mysid O. japonica at the shallow coast and juveniles of unidentified bivalve species at the deep coast were the favored food throughout the year. The delta C-13 and delta N-15 variations in C. uritai confirmed the spatial and temporal diet shift in gut content composition as the sand shrimp grow and migrate along the estuary-marine gradient.

Production and prey source of juvenile black rockfish Sebastes cheni in a seagrass and macroalgal bed in the Seto Inland Sea, Japan: estimation of the economic value of a nursery
Yasuhiro Kamimura, Akihide Kasai, Jun Shoji
AQUATIC ECOLOGY, 45, 3, 367, 376, SPRINGER, 2011年09月, ［査読有り］
英語, 研究論文（学術雑誌）, The economic value of ecosystem services of vegetated habitats (seagrass and macroalgal beds) has been estimated to be among the highest of the various ecosystems on earth. However, fish production has not been included in the evaluations due to the difficulty of quantitative estimation of fish production in the field. In the present study, annual production and economic value of wild juvenile black rockfish, Sebastes cheni, a dominant fish species in seagrass and macroalgal beds in the central Seto Inland Sea were estimated. Juvenile S. cheni migrated into vegetated habitats at about 20 mm total length (TL) and grew up to about 60 mm TL by late May. Juvenile abundance was highest in April (2007) or March (2008). Eight cohorts with the same extrusion period (each cohort covering a 10-day period) were identified using otolith daily increments. The annual estimates of S. cheni juvenile production were 13,080 g ha(-1) year(-1) in 2007 and 18,360 g (-1) year(-1) in 2008. Based on the unit price of artificially raised S. cheni juveniles, the economic value of the annual wild juvenile production was converted to 654,000 JPY (Japanese yen: 100 JPY = ca. 1 USD) ha(-1) year(-1) for 2007 and 918,000 JPY ha(-1) year(-1) for 2008. Analyses of stomach contents and stable isotope (delta(13)C or delta(15)N) showed that juvenile S. cheni were highly dependent on copepods. The planktonic trophic pathway originating from phytoplankton supports the majority of the juvenile black rockfish production during the post-migration period (20-60 mm TL) in the seagrass and macroalgal beds through the production of copepod zooplankton. The total economic value of the ecosystem services of the vegetated habitat is suggested to be much higher than estimates in previous studies if the provisioning and regulating services which originate from fish production were included in the estimates.

Migration ecology of juvenile temperate seabass Lateolabrax japonicus: a carbon stable-isotope approach
T. Fuji, A. Kasai, K. W. Suzuki, M. Ueno, Y. Yamashita
JOURNAL OF FISH BIOLOGY, 78, 7, 2010, 2025, WILEY-BLACKWELL, 2011年06月, ［査読有り］
英語, 研究論文（学術雑誌）, The migration pattern of juvenile temperate seabass Lateolabrax japonicus in the stratified estuary of the Yura River was examined using carbon stable-isotope ratios (delta(13)C). delta(13)C values of mysids, which are the most important prey items for this species, were consistently enriched in the lower estuary and surf zone (LES), while depleted in the freshwater zone (FW). delta(13)C values of juveniles in LES were enriched, while those in FW were depleted, consistent with the delta(13)C difference in prey items. The results of delta(13)C showed that many juveniles migrated upstream from April to June and most of them stayed in FW until at least July. Juveniles that stayed in FW and LES for a relatively longer period (> c. 20 days) showed higher condition factors than those that stayed in FW for a short period (several days). This indicates that residence in FW enabled juveniles to achieve as good a body condition as residence in LES. (C) 2011 The Authors Journal of Fish Biology (C) 2011 The Fisheries Society of the British Isles

Assessment of nitrogen loading from the Kiso-Sansen Rivers into Ise Bay using stable isotopes
Ryo Sugimoto, Akihide Kasai, Kouichi Fujita, Kenichi Sakaguchi, Tomomi Mizuno
JOURNAL OF OCEANOGRAPHY, 67, 2, 231, 240, SPRINGER, 2011年04月, ［査読有り］
英語, 研究論文（学術雑誌）, Concentrations of particulate organic nitrogen (PN), dissolved inorganic nitrogen (DIN), and their nitrogen isotope ratios (delta (15)N) in the Kiso-Sansen Rivers were determined from monthly observations over the course of a year to assess variations in the form and sources of riverine nitrogen discharged into Ise Bay. The delta (15)N values of NO(3) (-) observed in the Kiso-Sansen Rivers showed a logarithmic decreasing trend from 8 to 0aEuro degrees, which varied with the river discharge, indicating mixing between point sources with high delta (15)N and non-point sources with low delta (15)N. The influence of isotope fractionation of in situ biogeochemical processes (mainly DIN assimilation by phytoplankton) on delta (15)N of NO(3) (-) was negligible, because sufficient concentrations of NH(4) (+) for phytoplankton demand would inhibit the assimilation of NO(3) (-). A simple relationship between river discharge and delta (15)N of NO(3) (-) showed that the fraction of total NO(3) (-) flux arising from point sources increased from 4.0-6.3% (1.1-1.8 tN day(-1)) during higher discharge (> 600 m(3) s(-1)) to 30.2-48.3% (2.6-4.1 tN day(-1)) during lower discharge (< 300 m(3) s(-1)). Riverine NO(3) (-) discharge from the Kiso-Sansen Rivers can explain 75% of the variations in surface NO(3) (-) at the head of Ise Bay over the year.

平成22年度日本水産学会秋季大会を開催して
菅原 達也, 荒井 修亮, 農原 治彦, 吉田 天士, 左子 芳彦, 笠井 亮秀, 吉永 郁生, 荒井 修亮, 藤原 建紀, 山下 洋, 益田 玲爾, 澤山 茂樹, 木下 政人, 鰺坂 哲朗
日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries, 77, 1, 147, 149, The Japanese Society of Fisheries Science, 2011年01月15日
日本語

Migration ecology of juvenile temperate seabass Lateolabrax japonicus in the Yura River estuary: A carbon stable isotope approach　　　　　　　　　　　　　　　
Fuji T, Kasai A, Suzuki K, Ueno M, Yamashita Y
Journal of Fish Biology, 78, 2010-2025, 2011年, ［査読有り］
英語

Freshwater migration and feeding habits of juvenile temperate seabass Lateolabrax japonicus in the stratified Yura River estuary, the Sea of Japan
Taiki Fuji, Akihide Kasai, Keita W. Suzuki, Masahiro Ueno, Yoh Yamashita
FISHERIES SCIENCE, 76, 4, 643, 652, SPRINGER TOKYO, 2010年07月, ［査読有り］
英語, 研究論文（学術雑誌）, Juvenile temperate seabass Lateolabrax japonicus were sampled along the Yura River estuary from April to July 2008 to determine their distribution and feeding habits during migration within a microtidal estuary. Throughout the sampling period, juvenile seabass were distributed not only in the surf zone, but also in the freshwater zone, and they were particularly abundant in areas with aquatic vegetation in the freshwater zone. This distribution pattern suggests that the early life history of the temperate seabass depends more intensively on the river (freshwater) than previously considered. Small juveniles in the freshwater zone fed on copepods and chironomid larvae and upon reaching a standard length (SL) of approximately 20 mm did they fed on mysids. In contrast, juveniles (approx. 17-80 mm SL) in the surf zone fed mainly on mysids.

Distribution of moon jellyfish Aurelia aurita in relation to summer hypoxia in Hiroshima Bay, Seto Inland Sea
Jun Shoji, Takaya Kudoh, Hideyuki Takatsuji, Osamu Kawaguchi, Akihide Kasai
ESTUARINE COASTAL AND SHELF SCIENCE, 86, 3, 485, 490, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2010年02月, ［査読有り］
英語, 研究論文（学術雑誌）, Biological and physical surveys were conducted in order to investigate the relationship between environmental conditions and the distribution of moon jellyfish Aurelia aurita in Hiroshima Bay, western Seto Inland Sea, Japan. Moon jellyfish and ichthyoplankton were collected at 13 stations in Hiroshima Bay during monthly surveys from July to September in 2006 and 2007. Surface temperature in 2006 was significantly lower during the August and September cruises and surface salinity was lower during all cruises than in 2007. Moon jellyfish was the most dominant gelatinous plankton collected, accounting for 89.7% in wet weight. Mean moon jellyfish abundance in 2006 was higher than that in 2007 from July through September, with significant inter-year differences for July and September. Variability in precipitation and nutritional input from the Ohta River, northernmost part of Hiroshima Bay, were suggested as possible factors affecting the inter-annual variability in moon jellyfish abundance in the coastal areas of northern Hiroshima Bay. Moon jellyfish were more abundant in the coastal areas of northern Hiroshima Bay, where the dissolved oxygen (DO) concentration was lower, while low in the central part of the bay. Japanese anchovy Engraulis japonicus eggs were most dominant (58.1% in number) among the ichthyoplankton and were abundant in the central area of Hiroshima Bay. Explanatory analysis was conducted to detect possible effects of environmental conditions on the abundance of moon jellyfish and Japanese anchovy eggs during the summer months in Hiroshima Bay. Of the environmental conditions tested (temperature, salinity and DO of surface and bottom layers at each sampling station), bottom DO had the most significant effect on the moon jellyfish abundance: there was a negative correlation between the bottom DO and the moon jellyfish abundance in Hiroshima Bay during summer. (C) 2009 Elsevier Ltd. All rights reserved.

Modeling phytoplankton production in Ise Bay, Japan: Use of nitrogen isotopes to identify dissolved inorganic nitrogen sources
Ryo Sugimoto, Akihide Kasai, Toshihiro Miyajima, Kouichi Fujita
ESTUARINE COASTAL AND SHELF SCIENCE, 86, 3, 450, 466, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2010年02月, ［査読有り］
英語, 研究論文（学術雑誌）, An important aspect of the nitrogen cycle in coastal environments concerns the source of the nitrogen used in primary production. Phytoplankton production in Ise Bay, one of the most eutrophic embayments in Japan, is supported by external nitrogen derived from rivers and the ocean, and regenerated nitrogen formed in hypoxic water within the bay. We evaluated the contribution of each source of dissolved inorganic nitrogen (DIN) to phytoplankton production in Ise Bay. A unique three-dimensional ecosystem model including nitrogen isotopes (delta N-15) was developed based on precise observations. Model results revealed that DIN (=ammonium + nitrate) consumption by phytoplankton exceeds the DIN supply from the rivers and ocean, indicating that a large amount of phytoplankton production in Ise Bay depends on regenerated DIN within the bay rather than on newly supplied DIN. However, the ratio of consumption to external supply differs seasonally. Distributions of simulated delta N-15 clearly showed the source of nitrogen incorporated by phytoplankton in each Source. The intrusion depth of oceanic water changes from the bottom to the middle layer in spring. Oceanic nitrate is transported into the euphotic layer by the middle layer intrusion and stimulates phytoplankton production at the bay mouth. The subsurface chlorophyll maximum layer then develops. In autumn, however, the intrusion depth of oceanic water changes from the middle layer to the bottom layer. Regenerated NO3-, which is accumulated in the hypoxic water mass, is uplifted and continuously supplied to the euphotic layer. These results imply that phytoplankton production in Ise Bay is mainly dominated by the internal cycle rather than the external supply. (C) 2009 Elsevier Ltd. All rights reserved.

Salt-wedge intrusion of seawater and its implication for phytoplankton dynamics in the Yura Estuary, Japan
Akihide Kasai, Yoshiro Kurikawa, Masahiro Ueno, Dominique Robert, Yoh Yamashita
ESTUARINE COASTAL AND SHELF SCIENCE, 86, 3, 408, 414, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2010年02月, ［査読有り］, ［筆頭著者］
英語, 研究論文（学術雑誌）, We monitored hydrographic and biological conditions in the Yura Estuary and its coastal area from April 2006 to March 2008. Hydrographic conditions changed seasonally, and were mainly determined by river discharge and sea level, with tides playing a minor role. In summer, when river discharge was low and sea level was high, a salt-wedge regime was established until similar to 18 km upstream from the river mouth. Seawater intrusion into the river was however restricted in winter, when the river discharge was high and sea level was low. Water stratification caused by the seawater intrusion played an important role in phytoplankton dynamics. Phytoplankton developed in the middle layer of the estuary utilizing riverine nutrients, corresponding to the seawater intrusion into the bottom layer in summer. In winter, however, the phytoplankton production remained low in both estuary and sea. (C) 2009 Elsevier Ltd. All rights reserved.

Consumption of terrestrial organic matter by estuarine molluscs determined by analysis of their stable isotopes and cellulase activity
Emily S. Antonio, Akihide Kasai, Masahiro Ueno, Yoshiro Kurikawa, Kanako Tsuchiya, Haruhiko Toyohara, Yuka Ishihi, Hisashi Yokoyama, Yoh Yamashita
ESTUARINE COASTAL AND SHELF SCIENCE, 86, 3, 401, 407, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2010年02月, ［査読有り］
英語, 研究論文（学術雑誌）, We examined the feeding niche of four species of molluscs by analysis of their stable isotope signatures and cellulase activities to determine if they could utilize terrestrial organic matter. The molluscs and potential food sources were collected from the upper, middle and lower estuary of the Yura River from spring 2007 to winter 2008. All species showed positive cellulase activity which highlighted their potential to digest terrestrial organic matter. Consumption and assimilation of terrestrial organic matter by estuarine molluscs however varied spatially and temporally, reflecting species-specific differences in feeding niche and in response to variations in food availability in the estuary. Thus, terrestrial primary production in the catchment area supports secondary production of molluscs in the Yura River estuary. (C) 2009 Elsevier Ltd. All rights reserved.

Spatial variation in organic matter utilization by benthic communities from Yura River-Estuary to offshore of Tango Sea, Japan
Emily S. Antonio, Akihide Kasai, Masahiro Ueno, Nam-il Won, Yuka Ishihi, Hisashi Yokoyama, Yoh Yamashita
ESTUARINE COASTAL AND SHELF SCIENCE, 86, 1, 107, 117, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2010年01月, ［査読有り］
英語, 研究論文（学術雑誌）, We investigated the distribution of PC and delta N-15 of organic matter among benthic communities from the upper estuary of Yura River to offshore of Tango Sea, Japan, to determine spatial variation in utilization of organic matter by benthic communities. The delta C-13 values of benthic animals ranged from -27 to -15 parts per thousand in the upper estuary, -21 to -15 parts per thousand in the lower estuary, -20 to -16 parts per thousand in the shallow coast (5-10 m depths), -18 to -16 parts per thousand in the deep coast (30-60 m depths) and -19 to -15 parts per thousand in offshore (100-150 m depths) stations. Adapting the dual isotope values to mixing models, we estimated the relative contributions of potential food sources to the benthos diet. Phytoplankton and macroalgae that intruded the estuary in summer were utilized as alternative food aside from the terrestrial-origin organic matter assimilated by the estuarine benthic consumers. Resuspended benthic microalgae were important source of energy in the shallow coastal stations, while abundant supply of phytodetritus fueled the deep coastal and offshore benthic food webs. Spatial difference in the diet of benthic communities depends largely on the shifts in the primary carbon source. Thus, benthic communities are important link of autochthonous/allochthonous production and secondary production in the continuous river-estuary-marine system. (C) 2009 Elsevier Ltd. All rights reserved.

Controlling factors of seasonal variation in the nitrogen isotope ratio of nitrate in a eutrophic coastal environment
Ryo Sugimoto, Akihide Kasai, Toshihiro Miyajima, Kouichi Fujita
ESTUARINE COASTAL AND SHELF SCIENCE, 85, 2, 231, 240, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2009年11月, ［査読有り］
英語, 研究論文（学術雑誌）, Seasonal changes in the nitrogen stable isotope ratio of NO(3)(-) (delta(15)N(NO3)) were investigated in a temperate eutrophic coastal embayment in Japan. Wide variation of delta(15)N(NO3), from -10.4 to 9.3 parts per thousand was found along with changes in temperature, dissolved oxygen concentrations, and the composition of the dissolved inorganic nitrogen (DIN) pool. Throughout the year,delta(15)N(NO3), showed positive exponential and linear relationships with NO(3)(-) concentrations and fractions, respectively. The minima of delta(15)N(NO3) were observed in spring and autumn when the NO(3)(-) fraction in DIN was small, while the maximum was in summer when the NO(3)(-) fraction was large. These results indicate that nitrification was the dominant process that determined delta(15)N(NO3) variation. The delta(15)N(NO3) minima in spring and autumn suggested that hydrographic properties, such as low water temperature and short residence time of the water, suppressed the nitrifying bacterial activity in these seasons. An apparent nitrogen isotope discrimination (epsilon(nit)) by nitrification was estimated as -15 to -25 parts per thousand, which is similar to or slightly higher than previous laboratory estimates of marine nitrifiers. Furthermore, isotope results at the bay center over a year suggest that most of the NO(3)(-) was regenerated by in situ nitrification in the water-column. This implies that large amounts of regenerated nitrogen would lead the eutrophication in Ise Bay. (C) 2009 Elsevier Ltd. All rights reserved.

夏季の有明海筑後川の高濁度汽水域における粒状有機物の動態-河川流量の変動にともなう変化
鈴木 啓太, 杉本 亮, 笠井 亮秀, 中山 耕至, 田中 克
水産海洋研究, 73, 3, 149, 160, 2009年08月28日, ［査読有り］
日本語

Transport of oceanic nitrate from the continental shelf to the coastal basin in relation to the path of the Kuroshio
Ryo Sugimoto, Akihide Kasai, Toshihiro Miyajima, Kouichi Fujita
CONTINENTAL SHELF RESEARCH, 29, 14, 1678, 1688, PERGAMON-ELSEVIER SCIENCE LTD, 2009年07月, ［査読有り］
英語, 研究論文（学術雑誌）, Hydrographic and biogeochemical observations were conducted along the longitudinal section from Ise Bay to the continental margin (southern coast of Japan) to investigate changes according to the Kuroshio path variations during the summer. The strength of the uplift of the cold deep water was influenced by the surface intrusion of the Kuroshio water to the shelf region. When the intrusion of the Kuroshio surface water to the shelf region was weak in 2006, the cold and NO(3)(-)-rich shelf water intruded into the bottom layer in the bay from the shelf This bottom intrusion was intensified by the large river discharge. The nitrogen isotope ratio (delta(15)N) of NO(3)(-)(4-5 parts per thousand) in the bottom bay water was same as that in the deeper NO(3)(-) over the shelf, indicating the supply of new nitrogen to the bay. The warm and NO(3)(-)-poor shelf water intruded into the middle layer via the mixing region at the bay mouth when the Kuroshio water distributed in the coastal areas off Ise Bay in 2005. The regenerated NO(3)(-) with isotopically light nitrogen (delta(15)N = -1 parts per thousand) was supplied from the shelf to the bay. This NO(3)(-) is regenerated by the nitrification in the upper layer over the shelf. The contribution rate of regenerated NO(3)(-) over the shelf to the total NO(3)(-) in the subsurface chlorophyll maximum layer in the bay was estimated at similar to 56% by a two-source mixing model coupled with the Rayleigh equation. (C) 2009 Elsevier Ltd. All rights reserved.

Nitrogen isotope ratios of nitrate as a clue to the origin of nitrogen on the Pacific coast of Japan
Ryo Sugimoto, Akihide Kasai, Toshihiro Miyajima, Kouichi Fujita
CONTINENTAL SHELF RESEARCH, 29, 10, 1303, 1309, PERGAMON-ELSEVIER SCIENCE LTD, 2009年05月, ［査読有り］
英語, 研究論文（学術雑誌）, To clarify the sources and transformation of NO(3)(-) on the Pacific coast of Japan, observations over the continental shelf were conducted during the summer in 2005 and 2006 when the Kuroshio flowed close to and away from the coastal area, respectively. Below the halocline, there are two prominent salinity peaks that originated in two different waters. In the subsurface layer, the salinity maximum (S(max)) was indicative of the Kuroshio Water (KW), while the salinity minimum (S(min)) in the middle layer at similar to 400 m depth was indicative of the North Pacific Intermediate Water (NPIW). delta(15)N(NO3) ranged from 4.1 parts per thousand to 5.1 parts per thousand with a mean of 4.8 +/- 0.4 parts per thousand in the deeper water around S(min). Below 50m depth over the shelf break, delta(15)N(NO3) values (3.1 +/- 0.8 parts per thousand in 2005 and 4.6 +/- 0.3 parts per thousand in 2006) clearly increased as contribution of NPIW increased in 2006. On the contrary, subsurface delta(15)N of NO(3)(-) values (-1.1 +/- 0.1 parts per thousand) remained unchanged in both years, although the contribution of the KW to the subsurface water changed significantly. This Suggests that the source of NOT has little effect on the delta(15)N of NO(3)(-) in this layer. The negative delta(15)N values also coincided with the base of the chlorophyll maximum layer suggesting that these isotopic signals must be evidence of active nitrification in the upper layer. (C) 2009 Elsevier Ltd. All rights reserved.

Distinctive stable isotope ratios in important zooplankton species in relation to estuarine salinity gradients: Potential tracer of fish migration
Keita W. Suzuki, Akihide Kasai, Takane Isoda, Kouji Nakayama, Masaru Tanaka
ESTUARINE COASTAL AND SHELF SCIENCE, 78, 3, 541, 550, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2008年07月, ［査読有り］
英語, 研究論文（学術雑誌）, To assess the potential of stable isotope ratios as an indicator of fish migration within estuaries, stable isotope ratios in important zooplankton species were analyzed in relation to estuarine salinity gradients. Gut contents from migratory juveniles of the eurybaline marine fish Lateolabrax japonicus were examined along the Chikugo River estuary of the Ariake Sea, which has the most developed estuarine turbidity maximum (ETM) in Japan. Early juveniles in March and April preyed primarily on two copepod species; Sinocalanus sinensis at lower salinities and Acartia omorii at higher salinities. Late juveniles (standard length > 40 mm) at lower salinities preyed exclusively on the mysid Acanthomysis longirostris until July and complementarily on the decapod Acetes japonicus in August. These prey species were collected along the estuary during the spring-summer seasons of 2003 and 2004, and their carbon and nitrogen stable isotope ratios (delta(13)C and delta(15)N) were evaluated. The delta(13)C values of prey species were distinct from each other and were primarily depleted within and in close proximity to the ETM (salinity < 10); S. sinensis (-26.6 parts per thousand) < Acanthomysis longirostris (-23.3 parts per thousand) < Acartia omorii (-21.1 parts per thousand) < Acetes japonicus (-18.5 parts per thousand). The overall gradient of delta(13)C with salinity occurred for all prey species and showed minor temporal fluctuations, while it was not directly influenced by the delta(13)C values in particulate organic matter along the estuary. In contrast to delta(13)C, the delta(15)N values of prey species did not exhibit any clear relationship with salinity. The present study demonstrated that delta(13)C has the potential for application as a tracer of fish migration into lower salinity areas including the ETM. (c) 2008 Elsevier Ltd. All rights reserved.

Transport and survival processes of eggs and larvae of jack mackerel Trachurus japonicus in the East China Sea
Akihide Kasai, Kousei Komatsu, Chiyuki Sassa, Yoshinobu Konishi
FISHERIES SCIENCE, 74, 1, 8, 18, BLACKWELL PUBLISHING, 2008年02月, ［査読有り］, ［筆頭著者］
英語, 研究論文（学術雑誌）, Recent surveys showed substantial aggregation of larvae of jack mackerel in the southern East China Sea, indicating intensive spawning grounds near Taiwan. A numerical model was applied to investigate transport and survival processes of eggs and larvae of jack mackerel from the spawning area to the nurseries. The results show that: (i) the distributions of larvae simulated by the model agreed well with those obtained by field survey; (ii) the stock of jack mackerel in the Sea of Japan is composed of both groups from north of Taiwan and from the western coast of Kyushu. It takes more than two months for the former to reach the Sea of Japan, while it is within 40 days for the latter; and (iii) large proportions of the eggs and larvae spawned off the north of Taiwan are transported rapidly to the Pacific side of Kyushu by the Kuroshio Current, and the rest slowly to the east or north-east along the continental slope in the East China Sea. In contrast to the larval flux, survivors are more abundant in the northern East China Sea than in the Pacific Ocean, indicating that survival in the northern East China Sea would determine the jack mackerel stock in Japan.

Nitrogen isotopic discrimination by water column nitrification in a shallow coastal environment
Ryo Sugimoto, Akihide Kasai, Toshihiro Miyajima, Kouichi Fujita
JOURNAL OF OCEANOGRAPHY, 64, 1, 39, 48, SPRINGER, 2008年02月, ［査読有り］
英語, 研究論文（学術雑誌）, Temporal changes in nitrogen isotopic composition (delta N-15) of the NO3- Pool in the water column below the pycnocline in Ise Bay, Japan were investigated to evaluate the effect of nitrification on the change in the delta N-15 in the water column. The delta N-15 of NO3- in the lower layers varied from -8.5 parts per thousand in May to +8.4 parts per thousand in July in response to the development of seasonal hypoxia and conversion from NH4+ to NO3-. The significantly N-15-depleted NO3- in May most likely arose from nitrification in the water column. The calculated apparent isotopic discrimination for water column nitrification (epsilon(nit) = delta N-15(substrate) - delta(15) N-product) was 24.5 parts per thousand, which lies within the range of previous laboratory-based estimates. Though prominent deficits of NO3- from hypoxic bottom waters due to denitrification were revealed in July, the isotopic discrimination of denitrification in the sediments was low (epsilon(denit) = similar to 1 parts per thousand). delta N-15(NO3) in the hypoxic lower layer mainly reflects the isotopic effect of water column nitrification, given that water column nitrification is not directly linked with sedimentary denitrification and the effect of sedimentary denitrification on the change in delta N-15(NO3) is relatively small.

Combined stable carbon isotope and C/N ratios as indicators of source and fate of organic matter in the Bangpakong River Estuary, Thailand.　　　　　　　　　　　　　　　
Thanomsak Boonphakdee, Akihide Kasai, Tateki Fujiwara, Pichan Sawangwong, Voravit Cheevaporn
Environment Asia, 1, 28-36., 2008年, ［査読有り］
英語

Migration of Japanese temperate bass Lateolabrax japonicus juveniles within the Chikugo River estuary revealed by delta C-13 analysis
Keita W. Suzuki, Akihide Kasai, Taro Ohta, Kouji Nakayama, Masaru Tanaka
MARINE ECOLOGY PROGRESS SERIES, 358, 245, 256, INTER-RESEARCH, 2008年, ［査読有り］
英語, 研究論文（学術雑誌）, With conventional techniques it is difficult to evaluate the nursery value for migratory juveniles of Japanese temperate bass Lateolabrax japonicus within the Chikugo River estuary in Japan, especially in lower salinity areas where highly abundant prey organisms are distributed. In the present study stable carbon isotope ratios (delta C-13) were used as a migration tracer within the estuary. Based on preparatory information from diet-switch experiments and field observations of prey organisms, the ranges on which tissue VC values of juveniles should converge (convergence range) were determined in lower and higher salinity areas (LSA salinity < 10, HSA salinity > 10). For early juveniles, muscle delta C-13 values were analyzed and ages were determined by otolith increment counts. For more developed juveniles delta C-13 values were analyzed in muscle and liver, whose half-life values were 11.4 and 2.9 d, respectively. Temporal patterns of migration were estimated by comparing delta C-13 values in muscle and liver with the convergence ranges of both LSA and HSA. Early juveniles aggregated around the river mouth in March 2003 and the majority gradually ascended the river to LSA by April. Most of them stayed in LSA until August, while some began to expand their home ranges to HSA after July. In addition, it is suggested that the timing of ascent influenced the early growth of juveniles. Consequently, the potential importance of LSA was demonstrated during the first growth season of Japanese temperate bass in the estuary.

春季の有明海筑後川の高濁度汽水域における粒状有機物の動態
鈴木 啓太, 杉本 亮, 笠井 亮秀, 小路 淳, 中山 耕至, 田中 克
水産海洋研究, 71, 3, 190, 198, 水産海洋学会, 2007年08月, ［査読有り］
日本語

Cellulose digestion by common Japanese freshwater clam Corbicula japonica
Kentaro Sakamoto, Ken Touhata, Michiaki Yamashita, Akihide Kasai, Haruhiko Toyohara
FISHERIES SCIENCE, 73, 3, 675, 683, BLACKWELL PUBLISHING, 2007年06月, ［査読有り］
英語, 研究論文（学術雑誌）, Cellulose digestion by Corbicula japonica was investigated according to the hypothesis that without any symbiotic aid, this organism can utilize cellulose as a carbon source. Enzymatic studies revealed the complete cellulase activity of this species, and molecular cloning resulted in the isolation of cDNA with an ORF encoding a 596-amino-acid protein that shares significant homology with abalone and termite cellulases with an amino acid identity of 52.2% and 50.5%, respectively. The isolated cellulase had a carbohydrate-binding module at the N-terminal region that was also reportedly present in abalone cellulase, and its mRNA were specifically expressed in the digestive gland. These findings strongly support the assumption that C. japonica has an endogenous cellulose, as well as abalones and termites. It is further believed that C. japonica plays an important roll in decomposing cellulose, and consequently contributes to the carbon-cycle in the aquatic environment, as termites do in terrestrial forests.

Flow structure and hypoxia in Hiuchi-nada, Seto Inland Sea, Japan
Akihide Kasai, Tatsuo Yamada, Hiroshi Takeda
ESTUARINE COASTAL AND SHELF SCIENCE, 71, 1-2, 210, 217, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2007年01月, ［査読有り］
英語, 研究論文（学術雑誌）, Oxygen in the bottom water of the eastern basin of Hiuchi-nada. which is located in the central part of the Seto Inland Sea, Japan, is often depleted in summer. To clarify the formation mechanism of the hypoxic water mass, oxygen consumption rate experiments and detailed hydrographic observations were conducted. The experimental results showed that the oxygen consumption is unremarkable at the bottom in Hiuchinada, compared with other waters that show higher oxygen concentrations. From the hydrographic observations, the water is stratified and a cold dome, which corresponds to oxygen depleted water, is detected under the thermocline in the eastern basin of Hiuchi-nada. By contrast, outside of the dome, the water column is well mixed by strong tidal currents and the oxygen concentration is high. A prominent bottom front separates these two different water masses. There is a strong correlation between temperature and oxygen concentration under the thermocline (r(2) > 0.7). Residual currents from ADCP records show cyclonic circulation above the cold dome, while the water is nearly stationary inside the dome. Estimates of geostrophic currents from the density distribution are consistent with the observed flow pattern. These results indicate that the cold dome is isolated from the surrounding water Such that water exchange is insufficient, and thus the oxygen concentration reduces in Summer. Therefore, in Hiuchi-nada, the contribution of physical processes outweighs that of biochemical processes in the formation of the hypoxia. (c) 2006 Elsevier Ltd. All rights reserved.

内湾域における中層クロロフィル極大の形成機構
笠井亮秀, 杉本 亮, 赤嶺里美
海と空, 82, 3, 53, 60, 海洋気象学会, 2007年, ［査読有り］
日本語, 研究論文（学術雑誌）

Food sources for the bivalve Corbicula japonica in the foremost fishing lakes estimated from stable isotope analysis
A Kasai, H Toyohara, A Nakata, T Miura, N Azuma
FISHERIES SCIENCE, 72, 1, 105, 114, SPRINGER JAPAN KK, 2006年02月, ［査読有り］
英語, 研究論文（学術雑誌）, Carbon and nitrogen stable isotope ratios in tissue of the bivalve corbicula Corbicula japonica and particulate organic matter (POM) were measured in Lake Jusan, Lake Ogawara and Lake Shinji, which are the foremost fishing grounds for the corbicula in Japan, to determine their food sources. The bivalves in Lake Ogawara and Lake Shinji showed enriched isotope composition, while those in Lake Jusan were depleted. In addition, the difference in the isotope ratios between the sampling sites was remarkable in Lake Jusan. Chlorophyll concentrations were significantly higher in Lake Ogawara and Lake Shinji than those in the inflow rivers, although that in Lake Jusan was equivalent to that in the river. Residence time of river water was estimated at 1 day, 455 days and 88 days in Lake Jusan, Lake Ogawara and Lake Shinji, respectively. These values indicate that the bivalves in Lake Ogawara and Lake Shinji assimilate autochthonous phytoplankton, while those in Lake Jusan assimilate terrestrial matter in the upper reaches and marine phytoplankton in the lower reaches because of low production in the lake.

Short-term variation in behavior of allochthonous particulate organic matter accompanying changes of river discharge in Ise Bay, Japan
R Sugimoto, A Kasai, S Yamao, T Fujiwara, T Kimura
ESTUARINE COASTAL AND SHELF SCIENCE, 66, 1-2, 267, 279, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2006年01月, ［査読有り］
英語, 研究論文（学術雑誌）, The composition and behavior of allochthonous particulate organic matter (POM) in the northern part of Ise Bay, Japan were investigated to elucidate the short-term variation in POM accompanying changes in river discharge. The behavior of POM was significantly regulated by hydrographic conditions, but behavior was different in the upper layer versus the middle and lower layers. The former showed simple dynamics controlled by the river plume, while the latter showed complex dynamics because of changes in river discharge and subsequent variation in estuarine circulation. During normal discharge, the contribution of riverine materials to POC in the surface water within the bay was negligible because most riverine organic matter is deposited before flowing into the bay. During high discharge, on the other hand, the contribution of riverine organic matter to total POM increased to 50% at similar to 10 km from the river mouth. Though riverine organic matter loads increased, the total amount of POC decreased around the river mouth due to flushing of phytoplankton. After river discharge, the contribution decreased rapidly.
The behavior of POM in the middle and lower layers differed from that in the surface layer. At normal discharge, the influence of riverine organic matter was weak. During high discharge, high flooding temporarily weakened the bottom inflow, resulting in heavier riverine organic matter distributed from the river mouth to mid regions within the bay in the lower layer. The maximum contribution of riverine organic matter to total POM was estimated to be similar to 60% around 25 km from the river mouth. After high discharge, riverine POM in the lower layer was pushed to the bay head by enhanced estuarine circulation and was uplifted to the middle layer. The behavior of riverine POM dynamically changed in relation to fiver discharge, and exerted a significant influence on bottom water conditions in the bay. (c) 2005 Elsevier Ltd. All rights reserved.

Differential isotopic enrichment and half-life among tissues in Japanese temperate bass (Lateolabrax japonicus) juveniles: implications for analyzing migration
KW Suzuki, A Kasai, K Nakayama, M Tanaka
CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 62, 3, 671, 678, NATL RESEARCH COUNCIL CANADA, 2005年03月, ［査読有り］
英語, 研究論文（学術雑誌）, As a first step for field applications of stable isotope techniques to investigate the migration of Japanese temperate bass (Lateolabrax japonicus) (Perciformes) juveniles, we conducted a diet switch experiment and fitted an exponential model to changes in stable carbon (delta(13)C) and nitrogen (delta(15)N) isotope ratios for muscle, fin, and liver. The trophic enrichment values were ranked liver < muscle < fin for delta(13)C (range -0.80e to +3.66e) and liver < fin < muscle for delta(15)N (+0.59e to +3.12e). The half-life values were similar for muscle and fin for both delta(13)C and delta(15)N (19.3-25.7 days), while those for liver were 5.3 days for delta(13)C and 14.4 days for delta(15)N. Both the delta(13)C and delta(15)N values of muscle reached the asymptotic value after a threefold body weight increase, reflecting the diet after the switch. These results suggest that fin is a useful substitute for muscle in field applications of stable isotope techniques and that liver, with a shorter half-life, has the potential to provide more recent information about migration.

Utilization of terrestrial organic matter by the bivalve Corbicula japonica estimated from stable isotope analysis
A Kasai, A Nakata
FISHERIES SCIENCE, 71, 1, 151, 158, SPRINGER JAPAN KK, 2005年02月, ［査読有り］
英語, 研究論文（学術雑誌）, Carbon and nitrogen isotopic ratios in tissue of the bivalve corbicula (Corbicula japonica) and particulate organic matter (POM) were measured along a salinity gradient in the Kushicla Estuary, Japan. The bivalve exhibited a gradual isotopic enrichment from the uppermost estuarine site (delta(13)C = -24.8parts per thousand and delta(15)N = 8.6parts per thousand) to the marine site (delta(13)C = -16.1parts per thousand and delta(15)N = 11.8parts per thousand). Using the concentration-weighted mixing model, the bivalves' food source is estimated from the isotope values for the bivalves and POM from terrestrial plants, marine phytoplankton and benthic microalgae. The results indicated that the contributions of benthic micro algae and phytoplankton were small, while terrestrial particulate matter is significantly important for the corbicula diet, although the contribution varies among sampling sites.

Fortnightly shifts of intrusion depth of oceanic water into Ise Bay
A Kasai, T Fujiwara, T Kimura, H Yamada
JOURNAL OF OCEANOGRAPHY, 60, 5, 817, 824, TERRA SCIENTIFIC PUBL CO, 2004年10月, ［査読有り］
英語, 研究論文（学術雑誌）, To clarify the time change in water exchanges between Ise Bay and the adjacent ocean, repeated hydrographic observations were conducted along the longitudinal section in Ise Bay. The results show that the mixing condition at the bay mouth (Irago Strait) changed fortnightly in summer. During the spring tides, the strait water below the pycnocline was well-mixed and nearly homogeneous. By contrast, it was weakly stratified during the neap tide. There is a strong negative correlation between the tidal range and the density difference between the upper and lower layers at the strait. In summer, the intrusion depth of oceanic water into the bay and consequent hydrographic conditions inside the bay changed frequently according to the tidal strength. During the spring tides a prominent bottom front was created at the bay mouth, indicating that the strait water, which is a mixture of oceanic and bay waters, intruded through the middle layer. On the other hand, during the neaps, cold and saline oceanic water intruded through the bottom layer into the bay. The intrusion depth is significantly correlated with the tidal range. It is considered that the well-mixed strait water, which has a density equivalent to the middle layer inside the bay, is lighter than the bottom bay water and thus intrudes through the middle layer during the spring tides, while insufficient mixing makes the bottom water at the strait heavier than the bay water, leading to the bottom intrusion during the neap tides.

Selection of food sources by Ruditapes philippinarum and Mactra veneriformis (Bivalva : Mollusca) determined from stable isotope analysis
A Kasai, H Horie, W Sakamoto
FISHERIES SCIENCE, 70, 1, 11, 20, JAPANESE SOC FISHERIES SCIENCE, 2004年02月, ［査読有り］
英語, 研究論文（学術雑誌）, Carbon and nitrogen isotopic ratios in the body of the bivalves Ruditapes philippinarum and Mactra veneriformis were measured to estimate the role of the bivalves in the ecosystem in an estuary. The isotopic signatures were compared with those obtained from particulate organic matter (POM) in the middle reach of the river, in the estuary, and off the estuary. Both the bivalves showed constant delta(13)C and delta(15)N values of approximately -17parts per thousand. and 10parts per thousand. that were significantly enriched compared with those for POM in the habitat (-25parts per thousand. and 4parts per thousand.). The relative contributions of terrestrial organic matter to the POM in the habitat and to the bivalve diet were estimated: the former was approximately 90% while the latter approximately 10%. This indicates that the bivalves select marine POM from the organic matter available in their habitat, and play a minor role in the direct removal of terrestrial particulate matter. Temporal changes in delta(13)C and delta(15)N for both bivalves were small, but a distinct decrease in delta(13)C was observed following a period of heavy rain. During rainfall the concentration of terrestrial material increased in the habitat, and the bivalves would then have to change their food sources temporarily.

伊勢湾における河川流量の変動に伴う懸濁態有機物の変化　　　　　　　　　　　　　　　
杉本 亮, 笠井亮秀, 山尾理, 藤原建紀, 木村琢磨
水産海洋研究, 68, 3, 142, 150, 2004年, ［査読有り］
日本語, 研究論文（学術雑誌）

河川水と海水の接合点
笠井 亮秀
沿岸海洋研究, 40, 101, 108, 2003年, ［査読有り］
日本語

Offshore entrainment of anchovy larvae and its implication for their survival in a frontal region of the Kuroshio
Y Okazaki, H Nakata, S Kimura, A Kasai
MARINE ECOLOGY PROGRESS SERIES, 248, 237, 244, INTER-RESEARCH, 2003年, ［査読有り］
英語, 研究論文（学術雑誌）, Focusing on an incidence of coastal water entrainment into the Kuroshio frontal region, we looked at the fate of anchovy larvae Engraulis japonicus originating from coastal spawning grounds and entrained into the frontal region with the coastal low salinity water. Drifters were released in the low salinity water of the Kuroshio frontal region, and observation and sampling were conducted during drifter tracking from 18 to 21 May 1997. Naupliar copepod abundance and copepod production were high in the low salinity water at an early phase of the tracking, but decreased with elapsed days. Anchovy larvae were most abundant on the coastal side of the Kuroshio, especially in the low salinity water. Their ages estimated from otoliths were consistent with those inferred from the advective transport speed from the coastal spawning ground. The estimated biological mortality rate of first-feeding larvae (less than 7 d old) in the low salinity water was 0.71 d(-1), which was high compared to rates previously reported for Engraulis spp. This high mortality could have been caused by poor nutritional conditions due to decreasing copepod production and abundance, suggesting that the offshore entrainment of fish larvae originating from coastal water could be unfavorable for their survival. The encounter with a frontal eddy would enhance food production and the subsequent survival of anchovy larvae in the frontal region; otherwise, they may perish due to low food availability.

Entrainment of coastal water into a frontal eddy of the Kuroshio and its biological significance
Akihide Kasai, Shingo Kimura, Hideaki Nakata, Yuji Okazaki
Journal of Marine Systems, 37, 1-3, 185, 198, 2002年11月15日, ［査読有り］
英語, 研究論文（国際会議プロシーディングス）, The Pacific coastal areas of Japanese Island are major spawning grounds of various fishes. It is considered that large amount of eggs and larvae are dragged into the Kuroshio front so that the survival of fish larvae at the front is important for their recruitment. From this viewpoint, a low-salinity water mass, which was withdrawn from the coastal area to the Kuroshio front, was investigated by drifters, in addition to fine-scale hydrographic observations and water sampling in and around the Kuroshio frontal area off Enshu-nada. The drifters were transported to the east within the low-salinity water along the Kuroshio front in the first stage, and were thereafter entrained into an eddy, which was caused by the frontal meander. They moved closely to each other along the front, but diverged in the eddy. This movement of the drifters coincided with the deformation of low-salinity water mass
the low-salinity water concentrated at the Kuroshio front surrounded by strong salinity gradients at first, while it spread out horizontally and became vague in the shallow surface layer in the frontal eddy. Comparing temperature sections across the front, the strong upwelling was detected in the eddy. Limiting factors for primary production and growth rates were calculated in six sections using the observed temperatures and concentrations of nutrients. In the frontal area of the Kuroshio, low concentration of nutrients limited the primary production shallower than 50 m. Due to the low productivity, concentration of chlorophyll a in the low-salinity water tended to decrease, although the initial concentration was high. Once the coastal water mass was entrained into the frontal eddy, on the contrary, the concentration recovered due to the enhanced primary production in the subsurface layer supported by the upwelling of nutrient-rich water. Fish larvae in the low-salinity water are assumed to use the new production in the eddy
otherwise, they would starve. The entrainment process, which was probably caused by offshoreward movement of the Kuroshio, holds the key to successive survival and recruitment of fish larvae in the Kuroshio system. © 2002 Elsevier Science B.V. All rights reserved.

Circulation and cold dome in a gulf-type ROFI
A Kasai, T Fujiwara, JH Simpson, S Kakehi
CONTINENTAL SHELF RESEARCH, 22, 11-13, 1579, 1590, PERGAMON-ELSEVIER SCIENCE LTD, 2002年07月, ［査読有り］
英語, 研究論文（学術雑誌）, Recent surveys in Ise Bay, which is a major gulf-type region of freshwater influence in Japan, reveal that a cold dome is often observed in the stratified season. To elucidate the formation mechanism of the cold dome, detailed hydrographic and ADCP surveys were conducted in August 1995. The results show that a cold (T<20&DEG;C) water mass existed under the thermocline in the western basin of the bay. By contrast, in the middle layer of the eastern basin, there was mixed water, which exhibited characteristics equivalent to the water in the Irago Strait. A prominent bottom front separated these two different water masses. The ADCP results show a strong inflow in the form of a geostrophic current flowing above the bottom front, while the flow speed was weak in the cold water mass. The inflow area corresponded with the mixed water area, suggesting that the strait water would intrude into the bay along the eastern coast. In the western half of the basin, on the other hand, relict spring water is excluded from the exchange circulation and was trapped beneath the thermocline, where it formed the cold dome. The formation of the cold dome by this process is investigated using a three-dimensional model with two different density structures; a stratified and mixed region. The model results strongly support the formation mechanism of the cold dome, as the observed density and flow fields are successfully demonstrated. The key point to control the mechanism is the coexistence of the stratified and the mixed region. (C) 2002 Elsevier Science Ltd. All rights reserved.

The role of circulation in the development of hypoxia in Ise Bay, Japan
T Fujiwara, T Takahashi, A Kasai, Y Sugiyama, M Kuno
ESTUARINE COASTAL AND SHELF SCIENCE, 54, 1, 19, 31, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2002年01月, ［査読有り］
英語, 研究論文（学術雑誌）, Seasonally occurring hypoxia in Ise Bay, Japan was studied by comparing flow structures and ages of water parcels. Age is defined as the elapsed time after isolation of the water parcel from the oxygen source. In this bay, bay water is strongly stratified and advective transport by the estuarine gravitational circulation dominates from April to October. In contrast, the strong tidal current keeps the water column well mixed and oxygen-rich from top to bottom in the bay-mouth strait, which connects the bay with the Pacific Ocean. The estuarine circulation in Ise Bay is influenced by the effect of the Earth's rotation; the well-mixed water intrudes into the eastern half of the bay under the pycnocline with the land on its right (looking down current). Surrounded by this strait water, relatively cooler water (cold pool) remains on the bottom in the western half of the bay, isolated from the seasonal heating. Comparing the temperature of water under the pycnocline in the bay with the temperature of the strait water, we can estimate an age (tau) of water parcels. This age coincides with the mean residence time, which has traditionally been used. This method gives an age to the water parcel at a point under the pycnocline in the bay.
Oxygen concentration under the pycnocline indicates a high correlation coefficient (R-2 > 0.8) with age. The decrease of oxygen concentration during the period tau gives the oxygen consumption rate (OCR). The OCRs under the pycnocline were calculated from May to August. The OCR is approximately homogeneous independent of location in the bay. Moreover, monthly values of OCRs are also approximately constant from May to August, despite the mean temperature increase from 14.4 to 20.4 degreesC during this period. Known physical processes explain 80% of the spatial and temporal variation in oxygen concentration. In contrast, biochemical processes provide homogeneous OCR and play only a minor role in the variation in oxygen concentration. (C) 2002 Elsevier Science Ltd.

紀伊水道の海洋構造と栄養塩輸送
笠井亮秀, 藤原建紀, 多田光男
海岸工学論文集, 48, 1, 436, 440, Japan Society of Civil Engineers, 2001年10月20日, ［査読有り］
日本語

夏季の紀伊水道における残差流と生物輸送
笠井亮秀, 多田光男, 藤原建紀
海と空, 77, 2, 99, 106, 海洋気象学会, 2001年09月07日, ［査読有り］
日本語

伊勢湾の流動構造と貧酸素水塊
笠井亮秀, 川西里佳, 藤原建紀
水産海洋研究, 64, 4, 235, 243, 2000年11月30日, ［査読有り］
日本語

Behaviour of immature yellowtails (Seriola quinqueradiata) observed by electronic data-recording tags
A Kasai, W Sakamoto, Y Mitsunaga, S Yamamoto
FISHERIES OCEANOGRAPHY, 9, 3, 259, 270, BLACKWELL SCIENCE LTD, 2000年09月, ［査読有り］
英語, 研究論文（学術雑誌）, The long-term behaviour of immature yellowtails, Seriola quinqueradiata, was studied using electronic data-recording tags in Sagami Bay, Japan, during the autumn and early winter. Temperature or depth data were continuously recorded for 5-69 days on six yellowtails. The temperature recorded from the 0-year-old yellowtails showed only small variations (< 0.5 degrees C day(-1)) around the SST on the coast, indicating the 0-years stayed in the immediate coastal area with little horizontal movement. The 1-year-old yellowtail showed larger temperature variations that reached 3 degrees C day(-1), which implies distinctly larger horizontal movements than the 0-year-olds. Depth records showed the 0-years stayed around 10 m while the 1-year yellowtail swam in deeper water (mainly 10-30 m) with large variations. The latter often dived to 40 m in the daytime, but shallower than the depth of the thermocline. Both temperature and depth records showed apparent diurnal patterns. Comparing the fish data with environmental conditions, it is suggested that 1-year yellowtails stay close to the coast during the night, move at dawn to feed in the daytime and return at dusk via a frontal zone. In addition, wind stress could change yellowtails' behaviour.

Effect of the Earth's rotation on the circulation in regions of freshwater influence
A Kasai, AE Hill, T Fujiwara, JH Simpson
JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS, 105, C7, 16961, 16969, AMER GEOPHYSICAL UNION, 2000年07月, ［査読有り］
英語, 研究論文（学術雑誌）, Recent surveys in various regions of freshwater influence have shown considerably different now patterns in each region. An analytical model including viscous effects and the Earth's rotation is proposed to examine the along-channel flow pattern and to explain the differences. Model results show that the flow pattern is strongly dependent on the Ekman number E. With a large Ekman number (E > 1) the system is governed by gravitational circulation, and thus horizontal density gradients in along-channel direction are important. The whole water column is in the Ekman layer and consequent jet inflow penetrates the surface over a deep depression if the bottom topography varies in a cross-channel direction. With an intermediate Ekman number (E similar to 0.1) this jet inflow concentrates in the lower layer in which the viscosity still plays an important role. The flow in the upper layer is, on the other hand, determined by the geostrophic balance. The contribution by the geostrophic flow becomes larger so that the cross-channel density gradients are important when the Ekman number is small (E < 0.01). Since the Ekman layer is clung to the bottom, the jet inflow exists only in the thin bottom layer. The hydrographic and acoustic Doppler current profiler surveys were conducted in Ise Bay, Japan. Both the density structure and the flow pattern were different from those observed in many drowned river valleys, a strong jet inflow existed in the lower layer over the depression while the flow in the upper layer suggested anticyclonic circulation. The estimated Ekman number is 0.07 bn the bay and thus the observed pattern is consistent with the model result when E = 0.1.

Implications of meso-scale eddies caused by frontal disturbances of the Kuroshio Current for anchovy recruitment
H Nakata, S Kimura, Y Okazaki, A Kasai
ICES JOURNAL OF MARINE SCIENCE, 57, 1, 143, 151, ACADEMIC PRESS LTD, 2000年02月, ［査読有り］
英語, 研究論文（学術雑誌）, This paper discusses the implications of the meso-scale eddies, which are caused by frontal disturbances of the Kuroshio Current, for larval transport, distribution and food availability for Japanese anchovy, Engraulis japonicus (Houttuyn). It is a ubiquitous feature of the Kuroshio Front that wave-like meanders, with wavelengths of 100-400 km, generate cyclonic frontal eddies off the Pacific coast of the island of Japan. These eddies are likely to affect larval transport and the survival of the coastal spawning fish such as anchovy in the Kuroshio region by possibly moving coastal water offshore to the frontal region.
With this in mind, a frontal eddy was tracked from 18-23 May 1994 in the Enshu-nada Sea, where one of large spawning grounds of anchovy was found. Intensive transect surveys across the eddy were made to collect anchovy eggs and larvae simultaneously with prey organisms e.g. naupliar and copepodite copepods. These surveys showed that anchovy eggs and larvae hatched in the coastal water of the Enshu-nada Sea were entrained into the frontal eddy and transported along the Kuroshio front, possibly recruiting to coastal nurseries in the Enshu-nada Sea. In addition, the upwelling of nutrient-rich water in the vicinity of this cyclonic eddy enhanced primary production and subsequent copepod production, providing potentially favourable conditions for the feeding and growth of the anchovy larvae entrained in the eddy. (C) 2000 International Council for the Exploration of the Sea.

Density and flow structure in the Clyde Sea front
A Kasai, TP Rippeth, JH Simpson
CONTINENTAL SHELF RESEARCH, 19, 14, 1833, 1848, PERGAMON-ELSEVIER SCIENCE LTD, 1999年11月, ［査読有り］
英語, 研究論文（学術雑誌）, A well-defined front in temperature and salinity separates the stratified Clyde Sea water from the vertically well mixed water of the North Channel. The detailed structure of the front was observed in autumn 1990 by a combination of, repeated crossings of the front using a shipborne ADCP and a towed undulating CTD system, and the deployment of a fixed mooring system with temperature, salinity and velocity sensors for a period of 12 days. The results show that the front was situated on the Great Plateau near a contour of log(10)(H/U-2(3)) = 2.7 similar to 3.7 where H is the water depth and U-2 the amplitude of M-2 tidal velocity. The temperature structure in the Clyde Sea was inverted and the Clyde Sea surface temperature was lower than that of the vertically well mixed water in the North Channel. Since the salinity gradient was stronger than the temperature gradient with fresher water on the surface, the density structure was predominantly controlled by salinity. There were indications of warm and saline bottom water upwelling on the mixed side of the front during spring tides. This upwelling disappeared and the salinity and temperature structure at the front was more diffuse during the neap tide period. A jet-like along-front residual current was observed flowing to the northwest in the surface layer with a counter flow to the southeast in the bottom layer. The vertical difference in velocity was about 9 cm s(-1) and was approximately consistent with the shear determined from the thermal wind relation. Both cross- and along-front components of the current observed at the mooring station varied in response to the advection of the front, although both components had large variations with periods of less than one day and several days. The front was advected past the mooring system by a mean flow from the North Channel to the inner basin, while oscillating 3-5 km back and forth with the tidal currents. From the velocity at a current meter mooring and CTD data, the front was estimated to have moved up to 20 km during the observational period and the cross frontal velocity was inferred to be 3-4 cm s(-1). (C) 1999 Elsevier Science Ltd. All rights reserved.

一次生産量の時空間分布の算定手法
藤原建紀, 山尾理, 高橋鉄哉, 笠井亮秀, 杉山陽一, 原田一利
海岸工学論文集, 46, 2, 1211, 1215, 1999年10月20日, ［査読有り］
日本語

野外におけるマダイの酸素消費量の水温を指標とした見積もり
光永靖, 坂本亘, 荒井修亮, 笠井亮秀
日本水産学会誌, 65, 1, 48, 54, JAPAN SOC SCI FISHERIES SCI, 1999年01月, ［査読有り］, ［最終著者］
日本語, 研究論文（学術雑誌）, Two experiments were executed to investigate the correlation among water temperature, metabolic rate and swimming behavior of red sea bream Pagrus major. In the former experiment, the relationship between oxygen consumption and water temperature was measured in laboratory respirometry trials. The result shelved water temperature (T, degrees C) has the following correlation with oxygen consumption (OC, mg min.(-1) kg(-1)); OC=1.47+0.01x1.22(T), R=0.92. In the latter experiment, a wild adult fish was tracked in the open sea using an ultrasonic transmitter to record the horizontal migration paths, vertical swimming trajectory and ambient water temperature. The fish rested on the bottom during the day. While at night, it swam up and down within the depth range 30 to 80 m. The ambient water temperature ranged from 18.5 to 22.9 degrees C. In the period of resting, the red sea bream was estimated to consume oxygen ranging from 1.91 to 2.55 mg min.(-1) kg(-1), in the open ocean. During the experimental period of 28 days, the fish remained within a shoal and moved only small horizontal distances. Fluctuation of ambient water temperature seemed to have little effect on the swimming behavior of red sea bream.

マイクロデータロガーによるイナダの遊泳行動解析
笠井 亮秀
日本水産学会誌, 64, 2, 197, 203, Nihon Suisan Gakkai, 1998年, ［査読有り］
日本語, 研究論文（学術雑誌）, An acoustic transmitter and two micro data recording tags were used to observe the behavior of yellowtails Seriola quinqueradiata, in Sagami Bay, Japan from 24 October through 19 November. Depth data were continuously recorded for 23 days and temperature data for 27 days with a 20-minute interval. The yellowtails showed an apparent diurnal vertical migration pattern in their movements
they crossed the thermocline actively during the daylight hours, but calmed down and remained in the shallow layer above the thermocline at night. Their movements were changed by environmental temperature variation: 1) After the ambient water temperature rapidly decreased, a change was seen in the average depth and pattern in the vertical movement of the yellowtail. 2) They could move vertically to avoid the warm water from the Kuroshio because no ambient temperature rise and no shallower position were observed when the warm water intruded into the bay from the Kuroshio.

外洋から瀬戸内海に流入する窒素・リンの負荷量
藤原建紀, 宇野奈津子, 多田光男, 中辻啓二, 笠井亮秀, 坂本亘
海岸工学論文集, 44, 2, 1061, 1065, Japan Society of Civil Engineers, 1997年11月, ［査読有り］
日本語

紀伊水道の流れと栄養塩輸送
藤原建紀, 宇野奈津子, 多田光男, 中辻啓二, 笠井亮秀, 坂本亘
海と空, 73, 2, 63, 72, 海洋気象学会, 1997年09月
日本語

The dependence of yearly recruitment of Japanese sardine Sardinops melanostictus on survival in the Kuroshio-Oyashio Transition region
A Kasai, T Sugimoto, H Nakata
FISHERIES SCIENCE, 63, 3, 372, 377, JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES, 1997年06月, ［査読有り］
英語, 研究論文（学術雑誌）, We have numerically modeled the transport and survival processes of Japanese sardine eggs and larvae Sardinops melanostictus in the Kuroshio-Oyashio region to investigate the dependence of yearly recruitment of the sardine on the mortality rate during the early life stages. The model covers from 130 degrees E to 160 degrees E and simulates the Kuroshio Current and wind-induced current resulting from the winter monsoon in a simple way. As a biological process, natural mortality of the eggs and larvae is parameterized by area and year, considering the oceanographic condition such as the extent of southward intrusion of the Oyashio. The year-to-year variations in the amount of survivors simulated by the model are compared to that of one-year sardine stock based on the field data. The variation in the abundance of one-year old sardine is well correlated to that of the surviving juveniles off the east but not the south of Japan, both of which are calculated by the model. This indicates that the survival condition after the postlarval period off the east coast of Japan, which is strongly affected by the Oyashio, could play an important role in determining the year class strength. A strong year class can be formed by the numerous juveniles transported to the east and/or by the good survival condition in the eastern offshore region of Japan.

Biological productivity of meso-scale eddies caused by frontal disturbances in the Kuroshio
S Kimura, A Kasai, H Nakata, T Sugimoto, JH Simpson, JVS Cheok
ICES JOURNAL OF MARINE SCIENCE, 54, 2, 179, 192, OXFORD UNIV PRESS, 1997年04月, ［査読有り］
英語, 研究論文（学術雑誌）, Temporal and spatial changes in nutrient and chlorophyll distributions caused by frontal disturbances of the Kuroshio, a western boundary current in the Pacific Ocean, are presented. The regions of high nitrate and phosphate concentrations are associated with an eddy of positive vorticity which separates from the Kuroshio front within a few days of generation. The nutrients supplied to the euphotic layer by upwelling and mixing in the cyclonic eddy accelerate primary production in the frontal region. The specific growth rate calculated from temporal changes in chlorophyll and nitrate concentrations, of 0.8 d(-1) is considerably larger in the offshore region. According to a numerical simulation made using a turbulent closure model a chlorophyll maximum occurs during the fifth day after the generation of the eddy and the growth rate in the euphotic layer is in good agreement with that estimated from observations. After the peak production the growth becomes nutrient limited. Since the frontal disturbances occur in association with short-term fluctuations in the Kuroshio frontal meander, which has a period of a few weeks, the biological production enhancement by this kind of eddy is expected to occur with similar frequency, It is estimated that the total annual nitrogen input to the region via the eddies could result in a carbon production rate of 40 gC m(-2) y(-1). (C) 1997 International Council for the Exploration of the Sea.

Transport and survival of Japanese sardine larvae (Sardinops melanostictus) in the Kuroshio Oyashio region: A numerical approach
A Kasai, N Koizumi, T Sugimoto
SURVIVAL STRATEGIES IN EARLY LIFE STAGES OF MARINE RESOURCES, 219, 226, A A BALKEMA, 1996年, ［査読有り］
英語, 研究論文（国際会議プロシーディングス）

Influence of wind-induced currents on the recruitment of Japanese sardine (Sardinops melanostictus) eggs and larvae
KASAI A
Canadian Special Publication of Fisheries and Aquatic Sciences, 121, 547, 552, 1995年, ［査読有り］, ［筆頭著者］
英語, 研究論文（学術雑誌）

MIGRATION OF YELLOWTAIL IN RELATION TO INTRUSIONS OF WARM WATER FROM THE KUROSHIO
S KIMURA, A KASAI, T SUGIMOTO
FISHERIES SCIENCE, 60, 6, 635, 641, JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES, 1994年12月, ［査読有り］
英語, 研究論文（学術雑誌）, In the Kumano-nada and Enshu-nada Seas, warm water masses from the Kuroshio intrude into the coastal area depending on the paths type of the Kuroshio. Associated with the intrusions which occur with a period of 50 days from the Izu Peninsula when the Kuroshio takes the B-type path, yellowtail adults called buri migrate to the coastal area. However, the long time-lag between the intrusion and buri catch causes a negative relationship between the temperature and catch. The results suggest that the buri school migrates westward after the water mass has diffused. Since there is a positive relationship between temperature and catch associated with very rapid intrusions when the Kuroshio takes the C-type path, it is necessary to distinguish oceanographic phenomena in order to understand the fish behavior in response to offshore oceanic changes. In addition, the seasonal horizontal temperature gradient should be considered when estimating the optimum temperature range for fish because the relative temperature change greatly affects fish migration rather than the optimum temperature.

Warm water intrusion from the Kuroshio into the coastal areas south of Japan
Akihide Kasai, Shingo Kimura, Takashige Sugimoto
Journal of Oceanography, 49, 6, 607, 624, Kluwer Academic Publishers, 1993年11月, ［査読有り］, ［筆頭著者］
英語, 研究論文（学術雑誌）, Sea surface temperature (SST) has been measured in the south of Japan using a thermometer set up in the ferry boat to investigate the characteristics of the warm water intrudes into the coastal areas from the Kuroshio. Time series analysis was applied to the SST data with satellite images and hydrographic observation data from April 1987 to September 1989. The results indicate that the warm Kuroshio water intruded into the coastal areas on the Enshu-nada and the Kumano-nada Seas intermittently with periods of about 50 and 20 days associated with the fluctuation of the Kuroshio path and the Kuroshio frontal disturbance respectively. The intrusion with a 50-day period was dominant when the Kuroshio took a stationary small meander path (B- and C-types). The warm water spread to the west at 20 cm s-1, and was estimated to have a depth of 150 m at least and supply enough heat to make up the loss due to the evaporation in the coastal area. During the straight path of the Kuroshio, it was detected that the warm water intruded into coastal areas only with a 20-day period. The warm water that intrudes with a period of 20 days spreads to the west at 25 cm s-1 in a small scale. © 1993 Journal of the Oceanographic Society of Japan.

1次元モデルによるマイワシ卵稚仔の輸送・生残に関する研究
笠井 亮秀, 岸道郎, 杉本隆成
水産海洋研究, 56, 2, 99, 105, 水産海洋学会, 1992年04月, ［査読有り］
日本語, 研究論文（学術雑誌）

Modeling the transport and survival of Japanese sardine larvae in and around the Kuroshio Current
AKIHIDE KASAI, MICHIO J. KISHI, TAKASHIGE SUGIMOTO
Fisheries Oceanography, 1, 1, 1, 10, 1992年, ［査読有り］, ［筆頭著者］
英語, 研究論文（学術雑誌）, We have numerically modeled the advection and diffusion of sardine eggs and larvae to investigate the larval transport processes of Japanese sardine from the spawning grounds by the Kuroshio. The results indicated that the offshore drift current induced by the winter monsoon and the location of the spawning ground have significant effects on the survival of the Japanese sardine. The contribution of the drift current, the distance of the spawning ground from the Kuroshio axis, and the eddy diffusivity to the larval retention in the coastal area is approximately expressed by the following equation: where R is the retention rate in the coastal area, a the variance of initial distribution of eggs, T the time after the eggs were spawned, – V0 the velocity of the wind‐induced offshore current, y0 the distance of the center of the spawning area from the Kuroshio axis, and K the coefficient of horizontal eddy diffusivity. The year‐to‐year variation in larval survival rates stimulated by the two‐dimensional model are consistent with those estimated previously by using field data of egg and larval abundance during 1978–1988. Copyright © 1992, Wiley Blackwell. All rights reserved

黒潮親潮混合域における高気圧性渦が魚類分布に与える影響—Impact of anticyclonic eddy on fish distribution in the Kuroshio-Oyashio transition area
土橋 稜, 上野 洋路, 加藤 陸, 閻 乃筝, 向井 徹, 安間 洋樹, 野村 大樹, 伊藤 幸彦, 笠井 亮秀, 水産海洋研究 = Bulletin of the Japanese Society of Fisheries Oceanography, 88, 3, 178, 189, 2024年08月
東京 : 水産海洋学会, 日本語

夏の知床半島周辺海域における魚類相
櫻木雄太, 川上達也, 笠井亮秀, 山村織生, 三谷曜子, 日本水産学会大会講演要旨集(CD-ROM), 2023, 2023年

ニホンウナギの仔魚輸送シミュレーション-シラスは泳いで川を目指す!?-
唐木達郎, 坂本圭, 山中吾郎, 木村伸吾, 木村伸吾, 笠井亮秀, 水産海洋研究, 86, 2, 2022年

環境DNAを用いた夏期知床半島周辺海域における魚類群集の空間分布
櫻木雄太, 山崎彩, 笠井亮秀, 三谷曜子, 日本水産学会大会講演要旨集(CD-ROM), 2022, 2022年

瀬戸内海における河川の魚類多様性に与える環境要因と人間活動の影響
山崎彩, 村上弘章, 亀岡大真, 山中裕樹, 亀山哲, 久米学, 山下洋, 笠井亮秀, 日本水産学会大会講演要旨集(CD-ROM), 2022, 2022年

豊かな森林が河口域の生物多様性保全につながる~環境DNAメタバーコーディングによるアプローチ~
LAVERGNE Edouard, 久米学, AHN Hyojin, 邉見由美, 寺島佑樹, YE Feng, 亀山哲, 甲斐嘉晃, 門脇浩明, 小林志保, 山下洋, 笠井亮秀, 日本水産学会大会講演要旨集(CD-ROM), 2022, 2022年

黒潮親潮混合域における高気圧性渦の一次生産と魚類分布への影響
土橋稜, 上野洋路, 加藤陸, 閻乃筝, 向井徹, 安間洋樹, 野村大樹, 伊藤幸彦, 笠井亮秀, 日本水産学会大会講演要旨集(CD-ROM), 2021, 2021年

広域的な環境DNA解析によって捉えた魚類群集と海洋大規模循環構造との対応
川上達也, 山崎彩, 上野洋路, 兵藤晋, 笠井亮秀, 水産海洋学会研究発表大会講演要旨集, 2021, 2021年

環境DNA分析による全国の河川におけるニホンウナギ分布域の推定
笠井亮秀, 山崎彩, 安孝珍, 山中裕樹, 亀山哲, 益田玲爾, 東信行, 木村伸吾, 木村伸吾, 唐木達郎, 黒川優子, 山下洋, 日本水産学会大会講演要旨集(CD-ROM), 2021, 2021年

環境DNAによる森里海連環の解明~河口域魚類群集に底層溶存酸素濃度が及ぼす影響~
小林志保, 久米学, AHN Hyojin, 邉見由美, LAVERGNE Edouard, 寺島佑樹, YE Feng, YE Feng, 亀山哲, 甲斐嘉晃, 山下洋, 笠井亮秀, 日本水産学会大会講演要旨集, 2020, 2020年

陸域生態系が河川を通して沿岸生態系に与える影響評価のためのモデル構築
大庭ゆりか, 伊勢武史, 笠井亮秀, 亀山哲, AHN Hyojin, 久米学, 寺島佑樹, YE Feng, 山下洋, 日本生態学会大会講演要旨(Web), 67th, 2020年

気象庁海洋大循環モデルを用いたニホンウナギの仔魚輸送実験
唐木達郎, 坂本圭, 木村伸吾, 木村伸吾, 笠井亮秀, 日本海洋学会大会講演要旨集, 2020, 2020年

利根川水系とその周辺におけるチャネルキャットフィッシュの分布
山崎彩, 山中裕樹, 伊藤元裕, 木村伸吾, 笠井亮秀, 個体群生態学会大会プログラム・講演要旨集(Web), 36th, 2020年

Dispersion and degradation of environmental DNA from caged fish in a marine environment (vol 85, pg 327, 2019)
Hiroaki Murakami, Seokjin Yoon, Akihide Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Yoh Yamashita, Reiji Masuda, FISHERIES SCIENCE, 85, 6, 1109, 1109, 2019年11月
In the original publication the text in right column of page 330, the sequences of primers were incorrectly published as., SPRINGER JAPAN KK, 英語, その他

環境DNA を用いた全国河川のニホンウナギ分布調査 口頭
笠井 亮秀, 高田 真悟, 山崎 彩, 定行 洋亮, 木村 伸吾, 安 孝珍, 寺島 佑樹, 久米 学, 益田 玲爾, 山下 洋, 山中 裕樹, 今藤 夏子, 亀山 哲, 東 信行, 平成31年度日本水産学会春季大会, 2019, p.38, 2019年03月29日
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議）

福島県松川浦と流入河川におけるニホンウナギの摂餌生態 口頭
Alisa Kutzer, 小巻 拓平, 野田 琢嗣, 和田 敏裕, 久米 学, 寺島 佑樹, 堀 友彌, 高木 淳一, 三田村 啓理, 笠井 亮秀, 荒井 修亮, 山下 洋, 平成31年度日本水産学会春季大会, 2019, p.38, 2019年03月29日
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議）

環境DNAを用いた全国31河川河口域における魚類群集構造の解明
久米学, AHN Hyojin, 寺島佑樹, LAVERGNE Edouard, YE Feng, 亀山哲, 邉見由美, 甲斐嘉晃, 伊勢武史, 笠井亮秀, 山下洋, 日本魚類学会年会講演要旨, 52nd, 2019年

環境DNA分析によるエスチュアリーにおける魚類の多様性評価
笠井亮秀, 安孝珍, 久米学, 寺島佑樹, FENG Ye, 亀山哲, 宮正樹, 山下洋, 水産海洋学会研究発表大会講演要旨集, 2019, 2019年

環境DNA手法を用いた日本縁辺域におけるニホンウナギの分布可能性の解明
定行洋亮, 笠井亮秀, 山下洋, 東信行, 山中裕樹, 亀山哲, 久米学, 和田敏裕, 木村伸吾, 水産海洋学会研究発表大会講演要旨集, 2019, 2019年

環境DNAによるニホンウナギの分布域の推定 ポスター
笠井 亮秀, 高田 真悟, 山崎 彩, 定行 洋亮, 木村 伸吾, 安 孝珍, 寺島 佑樹, 久米 学, 益田 玲爾, 山下 洋, 山中 裕樹, 今藤 夏子, 亀山 哲, 東 信行, 2018年度水産海洋学会研究発表大会, 2018, p.83, 2018年11月17日
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議）

舞鶴湾におけるマアジとカタクチイワシの環境DNAの水平・鉛直分布 口頭　　　　　　　　　　　　　　　
村上 弘章, 山本 哲史, 源 利文, 南 憲吏, 宮下 和士, 深谷 肇一, 尹 錫鎭, 笠井 亮秀, 澤田 英樹, 鈴木 啓太, 益田 玲爾, 山下 洋, 近藤 倫生, 平成30年度日本水産学会春季大会, p.28, 2018年03月27日
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議）

環境DNA調査によるニホンウナギの淡水域分布特性の解明 口頭
定行 洋亮, 山中 裕樹, 久米 学, 山下 洋, 山崎 彩, 笠井 亮秀, 平成30年度日本水産学会春季大会, 2018, p.38, 2018年03月27日
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議）

東部瀬戸内海における基礎生産構造
安佛かおり, 宮原一隆, 山本圭吾, 秋山諭, 笠井亮秀, 日本生態学会大会講演要旨(Web), 65th, 2018年

流動モデルを用いた環境DNA濃度分布の再現　　　　　　　　　　　　　　　
笠井亮秀, 尹錫鎭, 海洋と生物, 234, 23, 27, 2018年
日本語

仙台湾におけるスズキの卓越年級群の形成要因 口頭　　　　　　　　　　　　　　　
外舘 香奈, 冨士 泰期, 高林 長将, 曽我部 共生, 鈴木 勇人, 栗田 豊, 山下 洋, 笠井 亮秀, 2017年度水産海洋学会研究発表大会, p.92, 2017年11月19日
日本語, 研究発表ペーパー・要旨（全国大会，その他学術会議）

異なるハビタットにおけるスズキ稚魚の生息密度と環境DNAの関係　　　　　　　　　　　　　　　
村上 弘章, 曽我部 共生, 鈴木 勇人, 源 利文, 笠井 亮秀, 鈴木 啓太, 山下 洋, 益田 玲爾, 2017年日本水産学会春季大会, p.33, 2017年03月28日
日本語

流域圏における農林水産業と地域振興-世界農業遺産国東半島・宇佐地域の森川里海-　　　　　　　　　　　　　　　
田村 勇司, 杉本 亮, 笠井 亮秀, 横山 寿, 山下 洋, 2017年日本水産学会春季大会, p.255, 2017年03月26日
日本語

Ⅱ-3. 由良川・丹後海における栄養塩，溶存鉄の動態と生物生産
渡辺 謙太, 福島 慶太郎, 笠井 亮秀, 吉岡 崇仁, 山下 洋, 日本水産学会誌, 83, 6, 1014, 1014, 2017年
公益社団法人 日本水産学会, 日本語, 速報，短報，研究ノート等（学術雑誌）

Numerical simulation of the transport of temperate seabass eggs and larvae in a region of freshwater influence 口頭　　　　　　　　　　　　　　　
Yoon, Seokj, Suzuki, Keita, W, Yamashita, Yoh, Kasai, Akihide, KOFFST International Conference 2017, 2017年
英語, 研究発表ペーパー・要旨（国際会議）

Comparison of aquatic biological production systems between two rivers in Oita Prefecture, Japan ポスター　　　　　　　　　　　　　　　
Kanzaki, Toko, Kume,Manabu, Hashiguchi, Shunya, Harada, Mami, Mochioka, Noritaka, Kasai, Akihide, Tamura, Yuji, Yokoyama, Hisashi, Arai, Nobuyuki, Yamashita, Yoh, 日本水産学会国際シンポジウム, SP05, 7, 2017年
英語, 研究発表ペーパー・要旨（国際会議）

A Lagrangian modeling approach for the transport of temperate seabass eggs and larvae in a region of freshwater influence 口頭　　　　　　　　　　　　　　　
Yoon, Seokj, Suzuki, Keita, W, Yamashita, Yoh, Kasai, Akihide, 日本水産学会国際シンポジウム, SO04, 23, 2017年
英語, 研究発表ペーパー・要旨（国際会議）

大分県国東半島における森里海連環‐陸起源懸濁物質の海域への流出　　　　　　　　　　　　　　　
橋口 峻也, 神崎 東子, 田村 勇司, 荒川 久幸, 山下 洋, 荒井 修亮, 笠井 亮秀, 平成28年度日本水産学会近畿支部例会, p.24, 2016年12月18日
日本語

大分県国東半島における森里海連環‐ニホンウナギを指標として‐　　　　　　　　　　　　　　　
神崎 東子, 橋口 峻也, 原田 真美, 鶴川 亮太, 望岡 典隆, 田村 勇司, 山下 洋, 荒井 修亮, 笠井 亮秀, 平成28年度日本水産学会近畿支部例会, p.25, 2016年12月18日
日本語

ウナギの石倉漁と資源保護に関する研究　　　　　　　　　　　　　　　
原田 真実, 神崎 東子, 橋口 峻也, 望岡 典隆, 鶴川 亮太, 清水 夏樹, 横山 寿, 田村 勇司, 笠井 亮秀, 山下 洋, 平成28年度日本水産学会近畿支部例会, p.26, 2016年12月18日
日本語

夏季の国東半島における河川水中の溶存無機態窒素・リンの動態の俯瞰的評価　　　　　　　　　　　　　　　
理塀隆人, 杉本亮, 田村勇司, 笠井亮秀, 山下 洋, 平成28年度日本水産学会中部支部会, p., 2016年12月03日
日本語

Commons management of estuarine ecosystem services: the case study for Japanese eels (Anguilla japonica)　　　　　　　　　　　　　　　
Harada,M, Onoue,M, Kanzaki,T, Hashiguchi,S, Tsurukawa,R, Mochioka,N, Tamura,Y, Shimizu,N, Kasai,A, Yamashita,Y, ECSA(Estuarine Coastal Shelf Association), GS3C O3-39, 2016年09月, ［査読有り］
英語

Connectivity of forests, rivers and seas −Relationship between land-use and aquatic biological production　　　　　　　　　　　　　　　
Kanzaki,T, Sogabe,T, Hashiguchi,S, Harada,M, Tsurukawa,R, Mochioka,N, Kasai,A, Tamura,Y, Yokoyama,H, Arai,N, Yamashita,Y, ECSA(Estuarine Coastal Shelf Association), GS3C O3-39, 2016年09月, ［査読有り］
英語

Connectivity of forests, rivers, and seas- Relation between land-use and water quality　　　　　　　　　　　　　　　
Hashiguchi,S, Kanzaki,T, Kasai,A, Tamura,Y, Sugimoto,R, Rihei,T, Arai,N, Yamashita,Y, ECSA(Estuarine Coastal Shelf Association), GS3C O3-39, 2016年09月, ［査読有り］
英語

夏季の大分県国東半島における河川水中の溶存無機態窒素・リンの動態　　　　　　　　　　　　　　　
理塀 隆人, 杉本 亮, 田村 勇司, 笠井 亮秀, 山下 洋, 2016年度地球惑星連合大会, A-CG23-03, 2016年05月24日
日本語

宇田賞受賞記念講演 沿岸域の物質循環と生物生産機構に関する研究
笠井 亮秀, 水産海洋研究 = Bulletin of the Japanese Society of Fisheries Oceanography, 80, 2, 146, 152, 2016年05月
水産海洋学会, 日本語

スズキ稚魚の成長に伴う成育場利用の変化　　　　　　　　　　　　　　　
笠井 亮秀, 曽我部 共生, 鈴木 勇人, 鈴木 啓太, 山下 洋, 平成28年度日本水産学会春季大会, p.40, 2016年03月27日
日本語

沿岸海域における環境DNAの分散過程に関する生簀を用いた検証実験
村上弘章, 尹錫鎭, 笠井亮秀, 源利文, 山本哲史, 坂田雅之, 堀内智矢, 澤田英樹, 益田玲爾, 日本水産学会大会講演要旨集, 2016, 35, 2016年03月26日
日本語

丹後海におけるスズキ仔稚魚の成育場の利用特性 ポスター　　　　　　　　　　　　　　　
曽我部 共生, 鈴木 勇人, 笠井 亮秀, 鈴木 啓太, 山下 洋, 日本生態学会第63回仙台大会, 2016年03月
日本語

海洋生態系モデリング Atlantisを用いた沿岸域生態系モデルの構築
竹茂愛吾, 伊藤幸彦, 笠井亮秀, YIN Shengle, YIN Shengle, 木村伸吾, 木村伸吾, 月刊海洋, 48, 7, 2016年

丹後海舞鶴湾におけるスズキ稚魚の成育場の利用特性　　　　　　　　　　　　　　　
曽我部 共生, 笠井 亮秀, 鈴木 啓太, 山下 洋, 荒井 修亮, 鈴木 勇人, 2015年度日本水産学会近畿支部後期例会, p.13, 2015年12月13日
日本語

国東半島における陸起源物質負荷量の降雨に伴う短期変動　　　　　　　　　　　　　　　
橋口 峻也, 笠井 亮秀, 田村 勇司, 山下 洋, 荒井 修亮, 2015年度日本水産学会近畿支部後期例会, p.21, 2015年12月13日
日本語

丹後海におけるスズキの初期生活史　　　　　　　　　　　　　　　
笠井亮秀, 冨士泰期, 曽我部共生, 政友明秀, 鈴木啓太, 山下洋, 東京大学大気海洋研究所共同利用集会「海洋における生物生産機構の多様性と連関−黒潮生態系から沿岸複合生態系まで−」, 2015年12月02日
日本語

沿岸域の循環と食物網動態：複合生態系モデリング　　　　　　　　　　　　　　　
伊藤 幸彦, 竹茂 愛吾, 笠井 亮秀, 尹 盛楽, 木村 伸吾, 東京大学大気海洋研究所共同利用集会「海洋における生物生産機構の多様性と連関−黒潮生態系から沿岸複合生態系まで−」, 2015年12月02日
日本語

複合生態研究における丹後海の流動環境　　　　　　　　　　　　　　　
木村 伸吾, 笠井 亮秀, 伊藤 幸彦, 銭本 慧, 竹茂 愛吾, 三宅 陽一, 鈴木 啓太, 山下 洋, 渡邊 良朗, 東京大学大気海洋研究所共同利用集会「海洋における生物生産機構の多様性と連関−黒潮生態系から沿岸複合生態系まで−」, 2015年12月01日
日本語

マナマコ浮遊幼生の湾内滞留機構について　　　　　　　　　　　　　　　
澤田 英樹, 山? 哲也, 上野 正博, 山下 洋, 笠井 亮秀, 2015年度日本ベントス学会・日本プランクトン学会合同大会, 2015年09月04日
日本語

沿岸複合生態系の構造と資源生物生産機能-４．スズキ稚魚による丹後海複合生態系の利用、口頭
山下洋, 笠井亮秀, 冨士泰期, 秋山諭, 渡辺謙太, 上野正博, 鈴木啓太, 平成32年度日本水産学会春季大会講演要旨集, 2015, p.63, 2015年03月
日本語

沿岸複合生態系の構造と資源生物生産機能-３．丹後海における基礎生産構造、口頭　　　　　　　　　　　　　　　
笠井亮秀, 安佛かおり, 尹錫鎭, 鈴木啓太, 山下洋, 渡辺謙太, 舟橋達宏, 平成31年度日本水産学会春季大会講演要旨集, p.63, 2015年03月
日本語

沿岸複合生態系の構造と資源生物生産機能-２．丹後海の流れとスズキ仔魚の輸送・生残 口頭
木村伸吾, 笠井亮秀, 伊藤幸彦, 銭本慧, 竹茂愛吾, 三宅陽一, 鈴木啓太, 山下洋, 渡邊良朗, 平成30年度日本水産学会春季大会講演要旨集, 2015, p.63, 2015年03月
日本語

東部瀬戸内海における基礎生産
安佛かおり, 笠井亮秀, 一見和彦, 山口一岩, 大美博昭, 秋山諭, 宮原一隆, 山本昌幸, 日本生態学会大会講演要旨(Web), 62nd, 2015年

東部瀬戸内海における基礎生産
安佛かおり, 一見和彦, 山口一岩, 大美博昭, 秋山諭, 宮原一隆, 山本昌幸, 笠井亮秀, 日本地球惑星科学連合大会予稿集(Web), 2015, 2015年

Atlantisを用いた沿岸域生態系モデルの構築
竹茂愛吾, 伊藤幸彦, 笠井亮秀, 尹盛楽, 木村伸吾, 水産海洋学会研究発表大会講演要旨集, 2015, 2015年

Artificial upwelling maintains favorable summer environment for farmed oysters　　　　　　　　　　　　　　　
Mizuta DD, Kasai A, Yamaguchi H, Nakata H, Global aquaculture advocate, 7, 16, 18, 2015年
英語, 記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）

生息環境ごとのスズキ仔稚魚の成長の差異とその要因、口頭　　　　　　　　　　　　　　　
曽我部共生, 高林長将, 冨士泰期, 鈴木啓太, 笠井亮秀, 山下洋, 2014年度水産学会近畿支部例会講演要旨集, p.8, 2014年11月22日
日本語

スズキ稚魚食性の海域間比較、口頭　　　　　　　　　　　　　　　
高林長将, 曽我部共生, 鈴木啓太, 笠井亮秀, 山下洋, 栗田豊, 2014年度水産学会近畿支部例会講演要旨集, p.9, 2014年11月22日
日本語

由良川河口から丹後海における基礎生産構造　　　　　　　　　　　　　　　
安佛かおり, 舟橋達宏, 渡辺謙太, 秋山諭, 上野正博, 笠井亮秀, 山下洋, 2014年度生物地球化学研究会講演要旨集, 2014年10月24日
日本語

沿岸性魚類スズキにおける河川・河口域の重要性　　　　　　　　　　　　　　　
冨士泰期, 鈴木啓太, 上野正博, 笠井亮秀, 山下洋, 2014年度生物地球化学研究会講演要旨集, 2014年10月24日
日本語

Relative contribution of estuarine nursery areas to the adult population of the temperate seabass Lateolabrax japonicus in Tango Bay, as revealed by otolith Sr:Ca ratios、口頭　　　　　　　　　　　　　　　
Taiki Fuji, Akihide Kasai, Masahiro Ueno, Yoh Yamashita, 5Th International otolith symposium. 要旨集, p.12, 2014年10月, ［査読有り］
英語

異なる河川におけるスズキ稚魚の摂餌生態と成長〜下流域におけるアミ類の分布と稚魚の摂餌〜 口頭　　　　　　　　　　　　　　　
桑原卓哉, 冨士泰期, 鈴木啓太, 笠井亮秀, 山下 洋, 岩本有司, 小路 淳, 平成26年度日本水産学会秋季大会講演要旨集, p.79, 2014年09月20日
日本語

由良川下流から河口域における基礎生産 口頭　　　　　　　　　　　　　　　
安佛かおり, 舟橋達宏, 石井健一郎, 笠井亮秀, 上野正博, 山下 洋, 日本陸水学会 第79回大会講演要旨集, p.90, 2014年09月10日
日本語

東北海域におけるホシガレイ仔稚魚の採捕記録と成長　　　　　　　　　　　　　　　
和田敏裕, 有瀧真人, 冨山毅, 中山耕至, 鈴木啓太, 笠井亮秀, 山下洋, 田中克, 平成26年度日本水産学会秋季大会講演要旨集, p.80, 2014年09月
日本語

由良川河口域におけるスズキ稚魚の摂餌生態と餌環境（口頭発表）　　　　　　　　　　　　　　　
冨士泰期, 笠井亮秀, 上野正博, 山下 洋, 平成26年度日本水産学会春季大会, p.93, 2014年03月28日
日本語

若狭湾西部海域における一次生産構造の季節変化 （口頭発表）　　　　　　　　　　　　　　　
舟橋達宏, 笠井亮秀, 鈴木啓太, 上野正博, 山下 洋, 平成26年度日本水産学会春季大会, p.137, 2014年03月28日
日本語

スズキ仔稚魚の成長に影響を及ぼす環境要因 （口頭発表）　　　　　　　　　　　　　　　
笠井亮秀, 青木貴志, 高林長将, 冨士泰期, 舟橋達宏, 栗田豊, 和田敏裕, 日比野学, 山下 洋, 平成26年度日本水産学会春季大会, p.92, 2014年03月28日
日本語

数値実験を用いた由良川河口域の低次生産過程の再現とその考察　　　　　　　　　　　　　　　
舟橋達宏, 笠井亮秀, 上野正博, 山下 洋, 木文化プロジェクト 03年度報告会, 2014年03月05日
日本語

耳石解析によるスズキ稚魚の回遊生態　　　　　　　　　　　　　　　
冨士泰期, 笠井亮秀, 上野正博, 山下 洋, 木文化プロジェクト 03年度報告会, 2014年03月05日
日本語

由良川−丹後海における栄養塩・懸濁態有機物の動態と基礎生産構造　　　　　　　　　　　　　　　
渡辺謙太, 笠井亮秀, 福?康司, 吉岡崇仁, 上野正博, 山下 洋, 木文化プロジェクト 03年度報告会, 2014年03月05日
日本語

由良川河口域における魚類群集と餌生物の季節変動　　　　　　　　　　　　　　　
青木貴志, 笠井亮秀, 冨士泰期, 上野正博, 山下 洋, 木文化プロジェクト 03年度報告会, 2014年03月05日
日本語

由良川下流域から丹後海における底生動物による有機物利用の空間変動　　　　　　　　　　　　　　　
Emily S. Antonio, 笠井亮秀, 上野正博, 横山寿, 山下 洋, 木文化プロジェクト 03年度報告会, 2014年03月05日
日本語

大村湾における人工的な湧昇が養殖マガキCrassostrea gigasの漁場環境と生育に及ぼす影響
MIZUTA Darien Danielle, 笠井 亮秀, 石井 健一郎, 水産海洋研究 = Bulletin of the Japanese Society of Fisheries Oceanography, 78, 1, 13, 27, 2014年02月
水産海洋学会, 英語

スズキ仔稚魚の成長に関する海域間比較 ‐耳石日周輪解析によるアプローチ‐ （口頭発表）　　　　　　　　　　　　　　　
青木貴志, 高林長将, 冨士泰期, 舟橋達宏, 栗田 豊, 和田敏裕, 日比野学, 笠井亮秀, 山下 洋, 2013年度水産学会近畿支部後期例会, 2013年12月07日
日本語

かわいい子には旅をさせよ 〜スズキの遡河群と沿岸群の初期生活史〜 （口頭発表）　　　　　　　　　　　　　　　
冨士泰期, 笠井亮秀, 上野正博, 山下 洋, 2013年度水産海洋学会シンポジウム, p.125, 2013年11月15日
日本語

スズキ稚魚に対する河川の役割 （口頭発表）　　　　　　　　　　　　　　　
冨士泰期, 笠井亮秀, 上野正博, 山下 洋, 第3回日本海研究集会 日本海の水産資源と環境・地域社会を考えるシンポジウム「みえる水（河川表流水）・みえない水（地下水）を通した陸域と海域のつながり」, 2013年11月09日
日本語

水産海洋アーカイブズ 赤潮と内湾環境 : 瀬戸内海を事例として
今井 一郎, 笠井 亮秀, 小路 淳, 水産海洋研究 = Bulletin of the Japanese Society of Fisheries Oceanography, 77, 39, 45, 2013年11月
水産海洋学会, 日本語

Mechanisms of river ascending by juvenile temperate seabass Lateolabrax japonicas ポスター　　　　　　　　　　　　　　　
Fuji T, Kasai A, Ueno M, Yamashita Y, 2013 Global Congress on ICM: Lessons learned to address new challenges, Marmaris, Turkey, 2013年11月, ［査読有り］
英語

Linkage between coastal phytoplankton production and river discharge in the Yura River Estuary, japan ポスター　　　　　　　　　　　　　　　
Watanabe K, Kasai A, Fukusaki K, Ueno M, Yamashita Y, 2013年11月, ［査読有り］
英語

Mechanisms of the river ascent of juveniles seabass　　　　　　　　　　　　　　　
Fuji T, Kasai A, Ueno M, Yamashita Y, 2013年11月, ［査読有り］
英語

Standing stocks and productivity of phytoplankton in the Yura River estuary ポスター　　　　　　　　　　　　　　　
Anbutsu K, Funahashi T, Ishii K, Kasai A, Ueno M, Yamashita Y, 2013年11月, ［査読有り］
英語

The effect of hydrographic conditions on the primary production in the Yura Estuary, Japan ポスター　　　　　　　　　　　　　　　
Funahashi T, Kasai A, Ueno M, Yamashita Y, 2013年11月, ［査読有り］
英語

弱混合型エスチュアリー由良川における基礎生産構造　　　　　　　　　　　　　　　
笠井亮秀, 安佛かおり, 舟橋達宏, 石井健一郎, 上野正博, 山下洋, 平成25年度日本水産学会春季大会, p.172, 2013年03月28日
日本語

日本海と瀬戸内海に流入する河川におけるスズキの初期生態比較　　　　　　　　　　　　　　　
桑原 卓哉, 冨士 泰期, 笠井 亮秀, 山下 洋, 一見 和彦, 岩本 有司, 荻野 裕平, 中野 光, 小路 淳, 平成25年度日本水産学会春季大会, p.67, 2013年03月27日
日本語

スズキ稚魚の河川遡上メカニズム - 2．水温との関係　　　　　　　　　　　　　　　
冨士泰期, 笠井亮秀, 上野正博, 山下洋, 平成25年度日本水産学会春季大会, p.67, 2013年03月27日
日本語

スズキ稚魚の河川遡上メカニズム - 1．塩水遡上との関係　　　　　　　　　　　　　　　
冨士泰期, 笠井亮秀, 上野正博, 山下洋, 平成25年度日本水産学会春季大会, p.67, 2013年03月27日
日本語

由良川河口域における植物プランクトンの基礎生産構造　　　　　　　　　　　　　　　
安佛 かおり, 舟橋 達宏, 石井 健一郎, 笠井 亮秀, 上野 正博, 山下 洋, 日本生態学会静岡大会, p.362, 2013年03月06日
日本語

大阪湾奥部底層水中の溶存無機態窒素の安定同位体比特性
杉本亮, 笠井亮秀, 山本圭吾, 日本海洋学会大会講演要旨集, 2013, 2013年

複合生態系モデリング:現状と課題
伊藤幸彦, 笠井亮秀, 木村伸吾, 木村伸吾, 水産海洋学会研究発表大会講演要旨集, 2013, 2013年

丹後海の流動構造と変動特性
銭本慧, 伊藤幸彦, 笠井亮秀, 木村伸吾, 木村伸吾, 日本水産学会大会講演要旨集, 2013, 2013年

Nitrogen dynamics in the Yodo River estuary, Japan
Sugimoto R, Kasai A, Proceedings of the 10th Global Congress on ICM: Lessons Learned to Address New Challenges, EMECS 2013 - MEDCOAST 2013 Joint Conference, 2, 777, 784, 2013年01月01日, ［査読有り］
The increase in human population in coastal watersheds has increased the delivery of nitrogen from the land to coastal environments. Accelerated nitrogen cycles in coastal environments have led to an increase in hypoxic waters and instances of harmful algal blooms. Physical and biogeochemical processes within estuaries generally regulate nitrogen fluxes from land to sea. The estuaries of major rivers on the continents are thought to be sites of massive nitrate losses. However, function of estuaries to nitrogen transfer must vary according to each estuarine hydrology and biogeochemistry. Osaka Bay is one of the most eutrophic embayments in Japan. A large amount of terrestrial nitrogen empties into the bay head from the Yodo River. Although the estuary would have a crucial role in modifying nitrogen fluxes, its function to nitrogen transfer is still unclear. In this study, we examined concentrations and stable isotope ratios (δ15N) of NO3- in the Yodo River Estuary to clarify the dominant biogeochemical processes on DIN dynamics within the estuary and to assess the effect of function of the estuary on eutrophication in Osaka Bay. Contrastive results were obtained from the observations during spring tides in May 2010 and 2011. Nitrate concentrations were high and δ15N was low when high salinity water (>30) intruded into the estuary in 2010. Most of the δ15N variations within the estuary were expressed by the Rayleigh accumulation model with isotope discrimination of nitrification, suggesting the importance of remineralisation for determinant of DIN concentration within the estuary. However, nitrate concentrations were low and δ15N was high when the high salinity., 英語, 記事・総説・解説・論説等（国際会議プロシーディングズ）

Subsurface Chlorophyll Maximum in a Stratified Estuary 口頭
Kasai A, Funahashi T, Anbutsu K, Ishii K, Ueno M, Yamashita Y, 2013 Global Congress on ICM: Lessons learned to address new challenges, Marmaris, Turkey, 807, 816, 2013年, ［査読有り］
Estuaries are formed around the river mouths, where lighter fresh water meets denser sea water. Many comparative studies have shown that production in estuaries is highest in marine systems, although there is a great deal of variety in mixing processes and salinity distributions. This study was conducted in the Yura Estuary, which is highly stratified because of the small tide, facing to the Sea of Japan. Seasonal and temporal variety of river discharge affects the dynamics in the estuary; the salt wedge intrudes into the bottom layer in summer when the river discharge is low. Consequent primary production is high in summer and a chlorophyll maximum is generated around the halocline, indicating that the salt wedge plays an important role in the ecosystem in the estuary.
A hydrodynamic-ecosystem coupled model was developed to investigate the formation mechanism of the chlorophyll maximum in the subsurface layer in the Yura Estuary. Salinity and chlorophyll distributions obtained by the model agreed well with field observations. The model results shows that in addition to the seasonal variation, the salt wedge responds over short time scales according to the flood events, and consequent distribution of chlorophyll frequently changes. Marine diatoms grow using a plenty of nutrients from the river when the salt wedge is formed in the bottom layer. However, production around the halocline is not always higher than the other layers. Direct measurements of the primary production in the Yura River showed the highest production in the surface layer, supporting the model results. These results indicate that the retention of advected phytoplankton is important for the formation of the chlorophyll maximum in the subsurface layer in the Yura Estuary., MEDITERRANEAN COASTAL FOUNDATION-MEDCOAST FOUNDATION, 英語

由良川河口域における魚類群集と餌生物の相互関係　　　　　　　　　　　　　　　
青木 貴志, 冨士 泰期, 笠井 亮秀, 上野 正博, 山下 洋, 2012年度水産学会近畿支部後期例会, 2012年12月01日
日本語

河川はスズキの生育場として重要なのか? −耳石Sr/Caによるアプローチ−　　　　　　　　　　　　　　　
冨士 泰期, 笠井 亮秀, 上野 正博, 山下 洋, 2012年度水産学会近畿支部後期例会, 2012年12月01日
日本語

由良川河口域における浮遊藻類の分布と一次生産について　　　　　　　　　　　　　　　
安佛 かおり, 舟橋 達宏, 笠井 亮秀, 上野 正博, 山下 洋, 日本陸水学会第77回大会, p.164, 2012年09月16日
日本語

3つの河川の下流?河口域におけるスズキ仔稚魚の摂餌生態比較　　　　　　　　　　　　　　　
桑原 卓哉, 一見 和彦, 岩本 有司, 冨士 泰期, 笠井 亮秀, 山下 洋, 荻野 裕平, 中野 光, 小路 淳, 平成24年度日本水産学会秋季大会, p.93, 2012年09月15日
日本語

河口域におけるヤマトシジミの成長と生残
野々大樹, 笠井亮秀, 山本圭吾, 酒井基介, 林浩志, 日本水産学会大会講演要旨集, 2012, 2012年

Influence of river flow on the dynamics of phytoplankton blooms during winter to spring in a micro-tidal estuary: Yura River Estuary, Japan　　　　　　　　　　　　　　　
Watanab,Kenta, Fukuzaki,Koji, Akiyama,Satoshi, Ichimi,Kazuhiko, Kasai,Akihide, Ueno,Masahiro, Yamashita,Yoh, ECSA 50, 2012年, ［査読有り］
英語

Relative contribution of the river nursery areas to temperate seabass Lateolabrax japonicus adult population revealed by otolith Sr:Ca ratio.　　　　　　　　　　　　　　　
Fuji,Taiki, Kasai,Akihide, Ueno,Masahiro, Yamashita,Yoh, 50th ECSA (Estuarine Coastal Science Association) Conference, 2012年, ［査読有り］
英語

シンポジウム「「里海」の学術的基礎」のまとめ(シンポジウム:「里海」の学術的基礎)
松野 健, 笠井 亮秀, 沿岸海洋研究, 48, 2, 95, 97, 2011年
日本海洋学会沿岸海洋研究部会, 日本語

ヨーロッパイガイ養殖場における好成長環境
森岡裕詞, 笠井亮秀, 北川貴士, 河村知彦, 木村伸吾, 水産海洋学会研究発表大会講演要旨集, 2011, 2011年

Temporal diet shift among benthic communities in a river-estuary-marine gradient　　　　　　　　　　　　　　　
Antonio,E. S, Ueno,M, Kasai,A, Ishihi,Y, Yokoyama,H, Yamashita,Yoh, Annual Conference of the South African Society of Aquatic Scientists, p.15, 2011年, ［査読有り］
英語

Use of salt-wedge intrusion by temperate seabass juveniles to ascend to upper estuary areas in the Yura River, Japan　　　　　　　　　　　　　　　
Fuji,T, Kasai,A, Ueno,M, Yamashita,Yoh, 21st Biennial Conference of the Coastal and Estuarine Research Federation, p.73, 2011年, ［査読有り］
英語

Feeding dynamics of macrobenthic communities from the Yura River-Estuary to Tango Sea　　　　　　　　　　　　　　　
Antonio,E.S, Ueno,Masahiro, Kasai,A, Ishihi,Y, Yokoyama,H, Yamashita,Yoh, 平成23年度日本水産学会春季大会講演要旨集, p.61, 2011年, ［査読有り］
英語

由良川河口域における塩水楔の短期変動に関する数値実験　　　　　　　　　　　　　　　
舟橋 達宏, 笠井 亮秀, 上野 正博, 山下 洋, 平成23年度日本水産学会春季大会講演要旨集,, p.60, 2011年
日本語

河川下流域における海洋構造とヤマトシジミの食性
笠井亮秀, 山本圭吾, 大美博昭, 佐野雅基, 酒井基介, 住友寿明, 西岡智哉, 岩本俊樹, 日本水産学会大会講演要旨集, 2010, 112, 2010年09月22日, ［査読有り］
日本語

Land-Sea Connection　　　　　　　　　　　　　　　
Antonio,E.S, Ueno,M, Kasai,A, Ishihi,Y, Yokoyama,H, Yamashita,Yoh, Key for Management of Estuary-Marine Systems?ECSA 47 Symposium , Book of Abstracts (Estuarine and Coastal Sciences Association), p.53, 54, 2010年, ［査読有り］
英語

京都府由良川河口域におけるスズキ稚魚の河川への回遊と塩水遡上との関係　　　　　　　　　　　　　　　
笠井亮秀, 2010年度水産海洋学会研究発表大会, 2010年, ［査読有り］
日本語

弱混合域における河川流量と塩水遡上の関係 -数値実験によるアプローチ-　　　　　　　　　　　　　　　
笠井亮秀, 2010年度水産海洋学会研究発表大会, 2010年, ［査読有り］
日本語

Spatial variability of food environment for bivalves in a narrow strait using stable isotope analysis　　　　　　　　　　　　　　　
Akihide Kasai, International symposium on isotope ecology 2010 in Kyoto: Relationship between biodiversity and ecosystem function, 2010年, ［査読有り］
英語

182. Migration and growth pattern of the temperate seabass Lateorabrax japonicus in the Yura River estuary revealed by δ13C　　　　　　　　　　　　　　　
Akihide Kasai, International symposium on isotope ecology 2010 in Kyoto: Relationship between biodiversity and ecosystem function, 2010年, ［査読有り］
日本語

Land-sea connection: Crucial for management of estuary-marine systems　　　　　　　　　　　　　　　
Akihide Kasai, Techno-Ocean 2010, 2010年, ［査読有り］
英語

Early life of the temperate seabass Lateolabrax japonicus　　　　　　　　　　　　　　　
Akihide Kasai, Techno-Ocean 2010, 2010年, ［査読有り］
英語

若狭湾由良川河口域におけるスズキ稚魚の生態　　　　　　　　　　　　　　　
笠井亮秀, 公開シンポジウム森里海連環と地球的課題, 2010年, ［査読有り］
日本語

Land-sea connection: Key for management of estuary-marine systems　　　　　　　　　　　　　　　
Akihide Kasai, ECSA (The Estuarine & Coastal Sciences Association) 47 symposium, 2010年, ［査読有り］
英語

十三湖におけるヤマトシジミ(Corbicula japonica)の減耗要因について　　　　　　　　　　　　　　　
笠井亮秀, 2010年度日本水産学会春季大会, 2010年, ［査読有り］
日本語

天然水域におけるヤマトシジミの成長要因の解明　　　　　　　　　　　　　　　
笠井亮秀, 2010年度日本水産学会春季大会, 2010年, ［査読有り］
日本語

河川流量の変動がヤマトシジミの成長に及ぼす影響　　　　　　　　　　　　　　　
笠井亮秀, 河川生態学術研究会第４回岩木川研究グループ発表会, 2010年, ［査読有り］
日本語

Food habits and migration pattern of the Japanese temperate bass Lateolabrax japonicus in the Yura River Estuary　　　　　　　　　　　　　　　
Akihide Kasai, International Symposium on Integrated Coastal Management for Marine Biodiversity in Asia, 2010年, ［査読有り］
英語

東シナ海におけるマアジ Trachurus japonicus 卵稚仔の輸送・生残過程
笠井 亮秀, 小松 幸生, 佐々 千由紀, 小西 芳信, 日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries, 75, 4, 615, 2009年07月15日
日本語

森と里と海のつながり 13.由良川に生息するメイオベントスのセルロース分解能の検討
豊原治彦, 土屋佳奈子, 笠井亮秀, 日本水産学会大会講演要旨集, 2009, 158, 2009年03月27日
日本語

SA-3 貧酸素水塊中に蓄積される無機態窒素の動態と富栄養化問題(Nitrogen Network-最新の実験・解析方法から新しい研究の切り口まで-, シンポジウム)
杉本 亮, 笠井 亮秀, 日本微生物生態学会講演要旨集, 25, 44, 44, 2009年
日本微生物生態学会, 日本語

Cosumption of organic matter by benthic communities determined by stable isotope analysis from downstream of Yura River to offshore of Tango Sea 森と里と海のつながり12． 安定同位対比分析による由良川下流〜丹後海におけるベントスの有機物利用　　　　　　　　　　　　　　　
Antonio, E.S, Ueno M, Kasai A, Kurikawa Y, Ishihi Y, Yokoyama H, Yamashita Y, 2010年度日本水産学会春季大会 講演要旨集, p.157, 2009年, ［査読有り］
英語

安定同位体比を用いたスズキ仔稚魚の回遊推定　　　　　　　　　　　　　　　
冨士 泰期, 笠井 亮秀, 鈴木 啓太, 上野 正博, 山下 洋, 2009年度水産海洋学会研究発表大会講演要旨集, p.32, 2009年
日本語

江戸・明治期の東京湾の物理環境と生態系
笠井亮秀, 2009年度水産海洋学会研究発表大会, 2009, 2009年, ［査読有り］
日本語

安定同位体比を用いた伊勢湾への河川起源窒素の負荷量評価　　　　　　　　　　　　　　　
笠井亮秀, 2009年度水産海洋学会研究発表大会, 2009年, ［査読有り］
日本語

海峡域の干潟に生息する二枚貝の摂餌に対する底生微細藻類の役割
笠井亮秀, 2009年度日本海洋学会秋季大会, 2009, 2009年, ［査読有り］
日本語

沿岸域における硝酸態窒素のd15Nの時空間変化
笠井亮秀, 2009年度日本海洋学会秋季大会, 2009, 2009年, ［査読有り］
日本語

由良川河口域の物理・化学・基礎生産過程　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

由良川河口域におけるスズキ仔魚の成長と生残　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

太田川河口域における魚類生産と餌起源の季節変化～河川・河口・海域の比較～　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

由良川に生息するメイオベントスのセルラース分解能の検討　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

由良川河口沿岸域における底生マクロベントスの食物構造　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

157. 由良川河口沿岸域におけるスズキ稚魚の出現と食性　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

由良川水系における懸濁態有機物と土地利用との関係　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

夏季の筑後川の高濁度汽水域における粒状有機物の動態　　　　　　　　　　　　　　　
笠井亮秀, 2009年度日本水産学会春季大会, 2009年, ［査読有り］
日本語

Diet shift in sand shrimp Crangon uritai as they migrate from marine to estuarine waters
ANTONIO Emily S, KASAI Akihide, UENO Masahiro, WON Nam‐Il, KAWAMURA Tomohiko, ISHIHI Yuka, MUKAI Hiroshi, YOKOYAMA Hisashi, YAMASHITA Yoh, 日本甲殻類学会大会講演要旨集, 47th, 127, 2009年, ［査読有り］
英語

ヤマトシジミによるセルロースの分解(平成19年度日本水産学会論文賞受賞)
坂本 健太郎, 東畑 顕, 山下 倫明, 笠井 亮秀, 豊原 治彦, 京都大学大学院農学研究科, 独, 水産総合研究センタ, 中央水産研究所, 独)水産総合研究センタ, 中央水産研究所, 京都大学大学院農学研究科, 京都大学大学院農学研究科, 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries, 74, 4, 569, 569, 2008年07月15日
日本語

安定同位体比を用いた餌料源の推定モデル
笠井亮秀, 安定同位体スコープで覗く海洋生物の生態-アサリからクジラまで, 46, 57, 2008年
恒星社厚生閣

δ¹⁵N結合生態系モデルによる伊勢湾の低次生産機構の解明-無機態窒素の挙動と一次生産の関係-
杉本亮, 笠井亮秀, 宮島利宏, 藤田弘一, 日本海洋学会大会講演要旨集, 2008, 2008年

陸棚域から伊勢湾への外海起源窒素の流入過程
杉本亮, 杉本亮, 笠井亮秀, 宮島利宏, 藤田弘一, 水産海洋学会研究発表大会講演要旨集, 2008, 2008年

安定同位体比を用いた陸棚域における硝酸態窒素の起源推定
笠井亮秀, 杉本亮, 杉本亮, 宮島利宏, 藤田弘一, 水産海洋学会研究発表大会講演要旨集, 2008, 2008年

英国メナイ海峡におけるヨーロッパイガイの生産機構の解明-2.安定同位体比による餌料環境の解析-
森岡裕詞, 木村伸吾, 笠井亮秀, 日本水産学会大会講演要旨集, 2008, 2008年

Origin of particulate organic matter in the Yura River, Japan　　　　　　　　　　　　　　　
Suzuki, K, Sugimoto R, Kasai A, Ueno M, Yamashita Y, EMECS (Environmental Management of Enclosed Coastal Seas)-8, Abstract, p.96, 97, 2008年, ［査読有り］
英語

Modeling phytoplankton production in Ise Bay, Japan -application of nitrogen isotopes to identification of DIN sources　　　　　　　　　　　　　　　
Sugimoto,Ryo, Kasai,Akihide, Miyajima,T, Fujita,K, EMECS8 (Shanghai, China), 2008年, ［査読有り］
英語

英国メナイ海峡におけるム‐ル貝（Mytilus edulis）の餌料環境の変動
笠井亮秀, 2008年度水産海洋学会研究発表大会, 2008, 2008年, ［査読有り］
日本語

藻場の魚類生産にもとづく生態系サービスの定量評価の試み－アマモ場におけるメバル仔稚魚の生産速度と餌起源の推定－　　　　　　　　　　　　　　　
笠井亮秀, 2008年度水産海洋学会研究発表大会, 2008年, ［査読有り］
日本語

安定同位体比を用いた大村湾における食物網解析　　　　　　　　　　　　　　　
笠井亮秀, 2008年度水産海洋学会研究発表大会, 2008年, ［査読有り］
日本語

Isotope evidence of seasonal variation in feeding niche of river and brackish gastropods　　　　　　　　　　　　　　　
Akihide Kasai, 2008 8th EMECS (Environmental Management of Enclosed Coastal Seas) conference, 2008年, ［査読有り］
英語

Modeling of phytoplankton production in Ise Bay, Japan: application of nitrogen isotopes to identification of DIN sources　　　　　　　　　　　　　　　
Akihide Kasai, 2008 8th EMECS (Environmental Management of Enclosed Coastal Seas) conference, 2008年, ［査読有り］
英語

Seasonal variation in seawater intrusion and primary production in the Yura Estuary, Japan　　　　　　　　　　　　　　　
Akihide Kasai, 2008 8th EMECS (Environmental Management of Enclosed Coastal Seas) conference, 2008年, ［査読有り］
英語

Increase in moon jellyfish populations in the Seto Inland Sea, Japan: Possible effect on predator-prey interactions under summer hypoxia　　　　　　　　　　　　　　　
Akihide Kasai, 2008 8th EMECS (Environmental Management of Enclosed Coastal Seas) conference, 2008年, ［査読有り］
英語

Ecosystem and biological production in Lake Jusan; nutrient cycles and spatiotemporal variability　　　　　　　　　　　　　　　
Akihide Kasai, 2008 5th world fisheries congress, 2008年, ［査読有り］
英語

Isotopic benthic community structure from downstream to offshore of Yura River　　　　　　　　　　　　　　　
Akihide Kasai, 2008 5th world fisheries congress, 2008年, ［査読有り］
英語

英国メナイ海峡におけるヨーロッパイガイの生産機構の解明 -1. 流動場からの考察-
笠井亮秀, 2008年度 海洋学会春季大会, 2008, 2008年, ［査読有り］
日本語

炭素・窒素安定同位体比分析及び飼育実験によるアイゴの摂餌選択性の解明　　　　　　　　　　　　　　　
笠井亮秀, 2008年度 海洋学会春季大会, 2008年, ［査読有り］
日本語

伊勢湾における無機態窒素の挙動と低次生産機構の解明－d15N結合生態系モデルによる低次生産機構の解明－　　　　　　　　　　　　　　　
笠井亮秀, 2008年度 海洋学会春季大会, 2008年, ［査読有り］
日本語

Hydrodynamics and ecosystem in the Yura Estuary
KASAI Akihide, ANTONIO Emily, KURIKAWA Yoshiro, UENO Masahiro, YAMASHITA Yoh, Program Abstr 5th World Fish Congr 2008, 250, 2008年, ［査読有り］
英語

Immunohistochemical, in situ hybridization, and biochemical studies on endogenous cellulase of Corbicula japonic
SAKAMOTO Kentaro, SAKAMOTO Kentaro, TOUHATA Ken, KASAI Akihide, TOYOHARA Haruhiko, Program Abstr 5th World Fish Congr 2008, 358, 2008年, ［査読有り］
英語

内湾への河川水の流入と一次生産
笠井 亮秀, 水産海洋研究, 71, 4, 299, 301, 2007年11月08日
水産海洋学会, 日本語

マアジ卵仔稚魚輸送予測モデルの開発—Numerical prediction of transport of eggs and larvae of jack mackerel—マアジ仔稚魚の初期生態と日本沿岸への輸送機構
小松 幸生, 笠井 亮秀, 月刊海洋 = Kaiyo monthly / 月刊海洋編集部 編, 39, 8, 550, 555, 2007年08月
海洋出版, 日本語

安定同位体比による汽水湖におけるヤマトシジミの食性の推定(平成18年度日本水産学会論文賞受賞)
笠井 亮秀, 豊原 治彦, 中田 晶子, 三浦 常廣, 東 信行, 京都大学農学研究科, 京都大学農学研究科, 京都大学農学研究科, 島根県水産技術センター, 弘前大学農学生命科学部, 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries, 73, 4, 646, 646, 2007年07月15日
日本語

シジミの内源性セルラーゼに関する研究
坂本健太郎, 笠井亮秀, 豊原治彦, 日本水産学会大会講演要旨集, 2007, 135, 2007年03月28日
日本語

高精度海況予測システムによるマアジ卵稚仔輸送・生残の年々変動特性—水産海洋地域研究集会 九州沖縄地区合同シンポジウム 東シナ海の黒潮・対馬暖流と生物資源
小松 幸生, 笠井 亮秀, 水産海洋研究 = Bulletin of the Japanese Society of Fisheries Oceanography, 71, 1, 53, 55, 2007年02月
水産海洋学会, 日本語

窒素安定同位体比に基づく伊勢湾における低次生産機構の解明
杉本亮, 笠井亮秀, 宮島利宏, 藤田弘一, 水産海洋学会研究発表大会講演要旨集, 2007, 2007年

伊勢湾における無機態窒素の安定同位体比特性
笠井亮秀, 杉本亮, 宮島利宏, 藤田弘一, 水産海洋学会研究発表大会講演要旨集, 2007, 2007年

伊勢湾における無機態窒素の挙動と低次生産機構の解明-陸棚域の硝酸態窒素の起源と挙動-
杉本亮, 笠井亮秀, 宮島利宏, 藤田弘一, 日本海洋学会大会講演要旨集, 2007, 2007年

伊勢湾の富栄養化と物質循環 (総特集 伊勢湾の貧酸素化と再生)
杉本 亮, 藤原 建紀, 笠井 亮秀, 海洋, 39, 1, 9, 14, 2007年01月
海洋出版, 日本語

Causes of the decrease of coastal fishery stocks in Japan, examination from ecological links from forest to coastal waters　　　　　　　　　　　　　　　
Yamashita,Y, Ueno,M, Kasai,A, International Symposium on Integrated Coastal Zone Management, Book of Abstracts, p.138, 2007年, ［査読有り］
英語

Formation mechanism of hypoxia in estuarine and coastal seas　　　　　　　　　　　　　　　
Kasai,A, Kurikawa,Y, Ueno,M, Yamashita,Y, International Symposium on Integrated Coastal Zone Management, Book of Abstracts, p.117, 2007年, ［査読有り］
英語

由良川水系における土地利用形態と水質の関係　　　　　　　　　　　　　　　
鈴木 健太郎, 杉本 亮, 笠井 亮秀, 上野 正博, 山下 洋, 田中 克, 平成19年度日本水産学会春季大会講演要旨集, p.240, 2007年
日本語

由良川水系における懸濁態有機物の挙動　　　　　　　　　　　　　　　
鈴木 健太郎, 杉本 亮, 笠井 亮秀, 上野 正博, 山下 洋, 平成19年度日本水産学会近畿支部後期例会要旨集, p.7, 2007年
日本語

由良川河口域の物理構造・一次生産構造の解明　　　　　　　　　　　　　　　
栗川 喜朗, 笠井 亮秀, 上野 正博, 山下 洋, 平成19年度日本水産学会春季大会講演要旨集, p.239, 2007年
日本語

シジミのセルロース分解プロセスに関する生化学的研究
坂本健太郎, 笠井亮秀, 坂上善人, 豊原治彦, 日本水産学会大会講演要旨集, 2006, 215, 2006年03月30日
日本語

7.安定同位体比から見た底生動物の餌料としての浮泥の役割(内湾浅海域浮泥の水産環境保全における重要性,日本水産学会水産環境保全委員会,懇話会ニュース)
笠井 亮秀, 京大院農, 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries, 72, 2, 283, 284, 2006年03月15日
日本語

伊勢湾における無機態窒素の挙動と低次生産機構の解明-硝化・脱窒に伴う窒素安定同位体比の変化-
杉本亮, 宮島利宏, 笠井亮秀, 藤田弘一, 日本海洋学会大会講演要旨集, 2006, 2006年

河口域潮下帯のヒトデ類は何を食べているか？　　　　　　　　　　　　　　　
坂本 三和, 上野 正博, 山下 洋, 笠井 亮秀, 林 勇夫, 平成18年度日本水産学会大会講演要旨集, p.106, 2006年
日本語

シジミにおける内在性セルラーゼの存在
豊原治彦, 高木雅哉, 林勇夫, 久保田賢, 東畑顕, 山下倫明, 笠井亮秀, 日本水産学会大会講演要旨集, 2005, 138, 2005年04月01日
日本語

有明海産スズキ稚魚の河川遡上生態－安定同位体比からのアプローチ－　　　　　　　　　　　　　　　
鈴木啓太, 笠井亮秀, 太田太郎, 礒田能年, 中山耕至, 田中 克, 2005年度日本水産学会春季大会, 2005年03月
日本語

餌生物の切り替えによるミズクラゲの炭素および窒素安定同位体比の経時変化　　　　　　　　　　　　　　　
木村 千秋, 笠井 亮秀, 益田 玲爾, 山下 洋, 日本水産学会近畿支部大会講演要旨集, 2005年
日本語

由良川河口域潮下帯におけるベントスの食物網　　　　　　　　　　　　　　　
坂本 三和, 上野 正博, 笠井 亮秀, 山下, 林 勇夫, 日本ベントス学会講演要旨集, 2005年
日本語

若狭湾潮下帯ベントスは陸上有機物を食べているか？　　　　　　　　　　　　　　　
坂本 三和, 上野 正博, 笠井 亮秀, 山下 洋, 林 勇夫, 北大-京大-琉大連携フィールド科学シンポジウム要旨集, p.24, 2005年
日本語

若狭湾2河口域に出現するクラゲ3種の生態特性-安定同位体比分析と胃内容物査定による食性解析-　　　　　　　　　　　　　　　
木村 千秋, 益田 玲爾, 笠井 亮秀, 上野 正博, 山下 洋, 田中 克, 北大-京大-琉大連携フィールド科学シンポジウム要旨集, p.15, 2005年
日本語

有明海産スズキ稚魚の生態解明への安定同位体比の応用－基礎情報としての濃縮係数と半減期の決定－　　　　　　　　　　　　　　　
鈴木啓太, 笠井亮秀, 太田太郎, 中山耕至, 田中 克, 2004年度日本水産学会春季大会, 2004年03月
日本語

Influence of changes in river discharge on the variation in particulate organic matter in Ise Bay.　　　　　　　　　　　　　　　
Sugimoto, R, Kasai, A, Yamao, S, Fujiwara, T, Kimura, T, Proceedings of International Symposium on Long-term Variations in the Coastal Environments and Ecosystems, 206, 211, 2004年
英語

海の栄養、陸の栄養　　　　　　　　　　　　　　　
笠井 亮秀, 瀬戸内海,31, 4-8, 2002年, ［査読有り］
日本語

Modeling the transport and survival of fish eggs and larvae.　　　　　　　　　　　　　　　
笠井 亮秀, GLOBEC Report,15, 49-51, 2002年, ［査読有り］
英語

イギリス,ウェールズで沿岸海洋学の研究に携わる　　　　　　　　　　　　　　　
笠井 亮秀, '99 大学院留学辞典,/,228-229, 1999年, ［査読有り］
日本語

Coastal Oceanography in Wales　　　　　　　　　　　　　　　
笠井 亮秀, ,/,228-229, 1999年, ［査読有り］
英語

ブイ追跡による遠州灘黒潮前線域の生物生産環境の研究 1. 物理・基礎生産
木村伸吾, 中田英昭, 岡崎雄二, 笠井亮秀, 杉本隆成, 日本水産学会大会講演要旨集, 1998, 1998年

遠州灘黒潮前線域の生物生産環境
木村伸吾, 中田英昭, 岡崎雄二, 笠井亮秀, 杉本隆成, 日本海洋学会大会講演要旨集, 1998, 1998年

酸素消費量と心拍数によるマダイの代謝と遊泳行動に関する研究 その2 バイオテレメトリーによる自然水域における遊泳行動の研究
光永靖, 坂本亘, 荒井修亮, 笠井亮秀, 日本水産学会大会講演要旨集, 1997, 89, 1997年04月
日本語

マイクロデタロガーによるイナダの遊泳行動解析 II
笠井亮秀, 坂本亘, 光永靖, 山本章太郎, 日本水産学会大会講演要旨集, 1997, 90, 1997年04月
日本語

魚卵・仔稚魚の輸送 -マイワシ資源変動との関連(共著)
笠井 亮秀, 海洋と生物,19/3,219-223, 19, 3, 219, 223, 1997年, ［査読有り］
生物研究社, 日本語

Transport of eggs and larvae with relation to the variation in sardine stock　　　　　　　　　　　　　　　
笠井 亮秀, ,19/3,219-223, 1997年, ［査読有り］
英語

マイクロデタロガーによるイナダの遊泳行動解析
笠井亮秀, 坂本亘, 光永靖, 山本章太郎, 日本水産学会大会講演要旨集, 1996, 48, 1996年03月
日本語

黒潮前線渦域における仔稚魚の分布と摂餌
岡崎雄二, 中田英昭, 木村伸吾, 笠井亮秀, 日本水産学会大会講演要旨集, 1996, 1996年

バイオテレメトリーによるイナダの遊泳行動解析　　　　　　　　　　　　　　　
光永 靖, 坂本 亘, 笠井亮秀, 山本章太郎, 海洋理工学会 平成8年度春季大会講演要旨集, 9, 1996年

マイワシ資源変動における物理環境の影響とそのモデル化(共著)　　　　　　　　　　　　　　　
笠井 亮秀, 月刊海洋,28/,98-103, 1996年
日本語

Effects of physical environment on the recruitment of sardine　　　　　　　　　　　　　　　
笠井 亮秀, Kaiyo monthly,28/,98-103, 1996年
英語

黒潮前線域の生物生産環境 生物生産機能
木村伸吾, 笠井亮秀, 中田英昭, 杉本隆成, 日本海洋学会大会講演要旨集, 1995, 1995年

黒潮前線域の生物生産環境 流動構造
笠井亮秀, 木村伸吾, 中田英昭, 杉本隆成, 日本海洋学会大会講演要旨集, 1995, 1995年

黒潮前線域の生物生産環境 卵・仔稚魚分布との対応
中田英昭, 岡崎雄二, 笠井亮秀, 木村伸吾, 日本海洋学会大会講演要旨集, 1995, 1995年

熊野灘・遠州灘への黒潮系暖水の流入(共著)
笠井 亮秀, 月刊海洋,26/11,703-707, 26, 11, 1994年
日本語

Intrusion of theKuroshio water into the Kumano-nada and Enshu-nada Seas　　　　　　　　　　　　　　　
笠井 亮秀, ,26/11,703-707, 1994年, ［査読有り］
英語

黒潮系水の沿岸域への流入に伴うブリの回遊について
笠井亮秀, 木村伸吾, 杉本隆成, 日本海洋学会大会講演要旨集, 1993, 1993年

いのちの循環「森里海」の現場から : 未来世代へのメッセージ72
笠井亮秀, 森と海をつなぐ河口域生態系を探る
花乱社, 2022年02月, 9784910038469, 351p, 日本語, ［共著］

環境DNA 生態系の真の姿を読み解く　　　　　　　　　　　　　　　
近藤倫生, 笠井亮秀, 清野聡子, 益田玲爾, 環境DNAを用いた大規模生態系観測の意義と展望
共立出版, 2021年03月, ［分担執筆］

豊かな内水面水産資源の復活のために－宍道湖からの提言　　　　　　　　　　　　　　　
ヤマトシジミの好適餌料の推定および数値シミュレーションによるヤマトシジミ浮遊幼生の輸送・生残実験
生物研究社, 2020年02月, ［共著］

Connectivity of Hills, Humans and Oceans: Challenge to Improvement of Watershed and Coastal Environments　　　　　　　　　　　　　　　
笠井 亮秀
京都大学出版会, 2014年, ［共編者(共編著者)］

水産海洋学入門　　　　　　　　　　　　　　　
笠井 亮秀, 貧酸素現象と青潮
講談社サイエンティフィック, 2014年, ［分担執筆］

海は百面相　　　　　　　　　　　　　　　
笠井 亮秀, 魚類の生態を読み解く新たなカギ
京都通信社, 2013年, ［分担執筆］

最新 水産ハンドブック　　　　　　　　　　　　　　　
笠井 亮秀, 潮汐流・干満・エスチャリー循環，高次食物連鎖
講談社サイエンティフィック, 2012年, ［分担執筆］

安定同位体スコープで覗く海洋生物の生態　　　　　　　　　　　　　　　
笠井 亮秀, 安定同位体比を用いた餌料源の推定モデル
恒星社厚生閣, 2008年, ［共著］

森川海のつながりと河口・沿岸域の生物生産　　　　　　　　　　　　　　　
笠井 亮秀, 河口・沿岸域での陸上有機物の挙動
恒星社厚生閣, 2008年, ［分担執筆］

Frontier Science Series No.48 (FSS-48)　　　　　　　　　　　　　　　
SUGIMOTO Ryo, KASAI Akihide, Changes in natural isotopic composition of nitrate during the development of hypoxia in Ise Bay, Japan
Universal Academic Press, 2007年, ［共著］

栽培システム学　　　　　　　　　　　　　　　
笠井 亮秀, アジアの栽培システム（水産業）
朝倉書店, 2005年, ［分担執筆］

水産海洋ハンドブック　　　　　　　　　　　　　　　
笠井 亮秀, 海水の物性
生物研究社, 2004年, ［分担執筆］

Oceanography　　　　　　　　　　　　　　　
笠井 亮秀
,, 2000年, 英語, 学術書

海洋学　　　　　　　　　　　　　　　
笠井 亮秀
2000大学院留学ガイド(]G0027[)環境学〜海外の環境学プログラム,, 2000年, 日本語, 学術書

黒潮・親潮混合域におけるマイワシ稚仔魚の輸送と生残(共著)　　　　　　　　　　　　　　　
笠井 亮秀
海洋生物の初期ステージにおける生き残り戦略,, 1996年, 日本語, 学術書

Transport and survival of Japanese sardine larvae in the Kuroshio-Oyashio region -A numerical approach-　　　　　　　　　　　　　　　
笠井 亮秀
Survival strategies in early life stages of marine resources,, 1996年, 英語, 学術書

環境DNAによるニホンウナギの分布域の推定　　　　　　　　　　　　　　　
笠井亮秀, 定行洋亮, 高田真悟, 安孝珍, 山崎彩, 久米学, 寺島佑樹, 東信行, 亀山哲, 木村伸吾, 山中裕樹, 益田玲爾, 山下洋
2018年度水産海洋学会研究発表大会, 2018年, 水産海洋学会
東京大学大気海洋研究所

水温と塩分がニホンウナギの環境DNA放出量に及ぼす影響　　　　　　　　　　　　　　　
高田真悟, 高橋さやか, 益田玲爾, 笠井亮秀
2018年度水産海洋学会研究発表大会, 2018年, 水産海洋学会
東京大学大気海洋研究所

環境DNA濃度の定量データと流動モデルの統合による個体数・生物量の推定　　　　　　　　　　　　　　　
深谷肇一, 村上弘章, 尹錫鎭, 南憲吏, 長田穣, 山本哲史, 益田玲爾, 笠井亮秀, 宮下和士, 源利文, 近藤倫生
第１回環境DNA学会東京大会, 2018年, 環境DNA学会
日本科学未来館

Evaluation of the fish distribution and their environmental DNA in a semi-closed bay using a three-dimensional tracer model　　　　　　　　　　　　　　　
Seokjin Yoon, 笠井亮秀, 村上弘章, 南憲吏, 益田玲爾, 宮下和士, 源利文, 近藤倫生
第１回環境DNA学会東京大会, 2018年, 環境DNA学会
日本科学未来館

環境DNA調査によるニホンウナギの淡水域分布特性の解明　　　　　　　　　　　　　　　
定行洋亮, 山中裕樹, 山下 洋, 久米 学, 山崎 彩, 笠井亮秀
平成30年度日本水産学会春季大会, 2018年, 日本水産学会, 日本語
東京海洋大学

舞鶴湾におけるマアジとカタクチイワシの環境DNA量の水平・鉛直分布　　　　　　　　　　　　　　　
村上弘章, 山本哲史, 源 利文, 南 憲吏, 宮下和士, 深谷 肇一, 尹 錫鎭, 笠井亮秀, 澤田英樹, 鈴木啓太, 益田玲爾, 山下 洋, 近藤倫生
平成30年度日本水産学会春季大会, 2018年, 日本水産学会, 日本語
東京海洋大学

東部瀬戸内海における基礎生産構造　　　　　　　　　　　　　　　
安佛かおり, 宮原一隆, 山本圭吾, 秋山諭, 笠井亮秀
第65回日本生態学会大会, 2018年, 日本生態学会, 日本語
札幌コンベンションセンター

由良川における河口域生態系研究から筑後川研究への展望I　　　　　　　　　　　　　　　
有明海森里海連環再生研究会, 2010年, 日本語, 口頭発表（招待・特別）

川の流れに支配される十三湖の生態系　　　　　　　　　　　　　　　
河川生態学術研究会第5回岩木川研究グループ研究発表会, 2010年, 日本語, 口頭発表（招待・特別）

アサリやシジミは水をきれいにしているのか？－安定同位体比分析による二枚貝の食性解析－　　　　　　　　　　　　　　　
2010. 第41回放射線科学研究会, 2010年, 日本語, 口頭発表（招待・特別）

十三湖におけるヤマトシジミの成長要因の解明　　　　　　　　　　　　　　　
平成20年度河川生態学術研究会岩木川グループ発表会, 2009年, 日本語, 口頭発表（招待・特別）

Removal of terrestrial organic matter by bivalves estimated from stable isotope analysis　　　　　　　　　　　　　　　
2008 University of Hawaii and University of Tokyo Joint Symposium on Ocean and Coastal Sciences, 2008年, 英語, 口頭発表（招待・特別）

陸棚域から伊勢湾への外海起源窒素の流入過程　　　　　　　　　　　　　　　
杉本亮, 杉本亮, 笠井亮秀, 宮島利宏, 藤田弘一
水産海洋学会研究発表大会講演要旨集, 2008年, 日本語

安定同位体比を用いた陸棚域における硝酸態窒素の起源推定　　　　　　　　　　　　　　　
笠井亮秀, 杉本亮, 杉本亮, 宮島利宏, 藤田弘一
水産海洋学会研究発表大会講演要旨集, 2008年, 日本語

Nutrients, chlorophyll and suspended solids from three rivers into Hakodate Bay, Hokkaido, Japan　　　　　　　　　　　　　　　
A. Kasai, R. Sugimoto, N. Itoh, K. Tsukagawa, K. Todate
ECSA 57, Estuarine Coastal Sciences Association, 英語
Pan Pacific Perth, Australia

海洋生物環境学　　　　　　　　　　　　　　　
京都大学農学部

生物圏環境学特論　　　　　　　　　　　　　　　
広島大学生物生産学部

海洋力学　　　　　　　　　　　　　　　
九州大学応用力学研究所

統計学　　　　　　　　　　　　　　　
近畿大学農学部

生態系モデリング　　　　　　　　　　　　　　　
北海道大学水産学部

海洋環境学　　　　　　　　　　　　　　　
北海道大学水産学部

海洋生物資源の変動要因の解明と高精度変動予測技術の開発　　　　　　　　　　　　　　　
2002年 - 2004年

環境DNAで北極海の海氷下の魚を探る
科学研究費助成事業 基盤研究(B)
2022年04月 - 2025年03月
笠井 亮秀, 野村 大樹
日本学術振興会, 基盤研究(B), 北海道大学, 22H02415

ウナギ属魚類の分布・来遊機構に与える地球環境変動および人為的環境改変の影響
科学研究費助成事業 基盤研究(A)
2021年04月 - 2025年03月
木村 伸吾, 笠井 亮秀, 萩原 聖士, 脇谷 量子郎
[輸送分散過程の数値シミュレーション解析] 主にEUのCopernicusが開発したデータ(GLOBAL OCEAN PHYSICS REANALYSIS)を使用して、オオウナギ北太平洋集団の産卵場（西マリアナ海嶺南部海域）からの仔魚輸送分散シミュレーションを実施した。その結果、北赤道海流によって西方に輸送された後、黒潮に取り込まれて北上するものとミンダナオ海流に取り込まれて南下する輸送経路を再現し、両者の割合には季節的・経年的変動があることを明らかにした。
[ニホンウナギとオオウナギを対象とした成魚の行動調査および人工構造物の影響評価] 養殖ニホンウナギの放流後の行動を調べるために、養殖ウナギ170個体を静岡県の深田川に放流した。携帯型RFIDシステムを用いて経時的追跡調査を実施したところ、上流移動が落差によって制限されることや、肥満度が低いほど下流移動確率が高まることが示された。また、静岡県の道龍川で採集したニホンウナギ49個体と奄美大島の大棚川で採集したオオウナギ106個体の水系内移送放流を行い、経時的追跡調査を実施した。その結果、10日後の元の生息地への回帰率はニホンウナギの場合には上流移送群が29.2%、下流移送群が16.0%、オオウナギの場合には上流移送群が3.7%、下流移送群が36.5%であり、種特異的な生息地固執性・選好性の存在が示唆された。
[環境DNA分析のための採水・濾過および解析] 日本産ウナギ属魚類4種を対象に、環境DNA検出/定量用の種特異的プライマー・プローブセットを設計した。房総半島、屋久島、奄美大島、石垣島、西表島の20河川、計51地点において採水を行い、試料を収集した。また、南西諸島海域で行った学術研究船「白鳳丸」による調査データの遺伝子解析を継続して実施し、その際に採集した仔稚魚の種同定結果との比較を行う準備を進めた。
日本学術振興会, 基盤研究(A), 東京大学, 21H04939

環境DNAを用いた全国の河川におけるニホンウナギの分布・生息量推定
科学研究費助成事業 基盤研究(A)
2017年04月 - 2021年03月
笠井 亮秀, 東 信行, 益田 玲爾, 山下 洋, 山中 裕樹, 亀山 哲, 木村 伸吾
本研究では，環境DNAを用いて，日本全国の河川において，絶滅危惧種に指定されているニホンウナギの分布を確定し，そのバイオマスを推定することを目的としている。


今年度も，昨年度に引き続き，全国の河川下流～河口域において採水を行った。2019年度は，167ヶ所で採水した。環境条件として，水温，電気伝導度，川幅，河口からの距離も同時に測定した。採水サンプルから水中有機物のDNAを抽出し，2017年度に作成したニホンウナギの種特異的プライマーを用いてDNA分析を行った。環境DNAの分布と環境条件との関係を解析したところ，日本国内の河川におけるニホンウナギの分布やバイオマスは，ほぼ仔魚期の輸送環境によって決まることが分かった。そこで産卵場から卵稚仔の輸送をシミュレーションしたところ，地球温暖化に伴って将来日本へのウナギ仔魚の輸送が減少する可能性も示唆された。
また堰が存在する河川においては堰の上流側と下流側で採水とウナギのサンプリングを行い，環境DNAを分析した。その結果，堰がニホンウナギの遡上を妨げており，その分布に影響を及ぼしていることが明らかとなった。特に200mm以下の小型の個体は，堰が増えるにつれその上流側で個体数が有意に少なくなり，小型個体は堰を超えて遡上しづらい状況にあることが分かった。
上記に加えて，環境DNAの分解に関する実験を行った。ウナギを10℃～30℃ の5つの水温区に保った水槽に入れて飼育したのち，水槽から水を取り出し6日間保管した。その間，毎日環境DNA濃度を測定した。その結果，環境DNA濃度の減衰率kと水温Tとの関係はk = 0.02T + 0.18で表され，水温が高いほど分解率が速いことが分かった。
日本学術振興会, 基盤研究(A), 北海道大学, 17H01412

森から海までの生態系連環機構の解明によるニホンウナギ資源の再生
科学研究費助成事業
2016年04月01日 - 2020年03月31日
山下 洋, 望岡 典隆, 笠井 亮秀, 木村 伸吾, 杉本 亮, 荒井 修亮
大分県、和歌山県、福島県の河川において、ニホンウナギの生産を支える環境と生態系を流域レベルで調べ、ウナギの資源生産の観点から河川再生方策を検討した。ウナギの食性は、サイズ、季節、地域で変化したが、基本的に広食性で柔軟な摂餌生態を有した。全長200mm未満の小型魚と200mm以上の大型魚では、好適な生息環境特性が異なった。ウナギの河口から河川上流への移動・拡散は堰により阻害された。本種は水質の許容範囲が広く、清流から都市型河川まで生息した。ウナギの河川内及び河川と沿岸間の移動、隠れ場、餌生物の生産を可能にする一定の環境条件整備により、本種資源の保全と培養が可能であると考えられた。
日本学術振興会, 基盤研究(A), 京都大学, 16H02563

環境DNA分析に基づく魚類群衆の定量モニタリングと生態系評価方法の開発　　　　　　　　　　　　　　　
CREST
2013年 - 2018年
近藤 倫生
科学技術振興機構, 競争的資金

森里海の連環を基盤とした食料生産機構の解明と地域振興策の検討
科学研究費助成事業
2014年07月18日 - 2017年03月31日
山下 洋, 横山 壽, 杉本 亮, 清水 夏樹, 吉岡 崇仁, 笠井 亮秀
豊かな森林に恵まれた国東半島を流域とする桂川では、森林土壌由来の栄養塩が河川に供給され、それらが食物網を通して河口域の生物多様性やスズキ稚魚、ニホンウナギの生産に貢献する可能性が示された。森林には、水圏生態系に有害な微細粒子（＜30μm）の河川への供給を抑制する効果も認められた。世界農業遺産に認定された国東半島・宇佐地域では、河川下流域でニホンウナギを漁獲するための石倉漁が行われている。ウナギ石倉は河口域に加入する小型ウナギに好適な生息場を提供し、餌料生物を生産する機能をもつとともに、世界農業遺産において地域の文化的景観を保全し人と自然をつなぐ重要な社会的役割を有することがわかった。
日本学術振興会, 基盤研究(B), 京都大学, 26310308

沿岸海域複合生態系の変動機構に基づく生物資源生産力の再生・保全と持続的利用に関する研究　　　　　　　　　　　　　　　
国家基幹研究開発推進事業 海洋資源利用促進技術開発プログラム
2011年 - 2017年
渡邊 良朗
文部科学省, 競争的資金

貧酸素化が進行する閉鎖性内湾の環境修復：大村湾をシミュレーターとした検証実験
科学研究費助成事業
2010年04月01日 - 2014年03月31日
中田 英昭, 松岡 數充, 梅澤 有, 和田 実, 笠井 亮秀, 山口 仁士, 杉本 亮
夏季に底層水の貧酸素化が恒常的に進行する大村湾をシミュレーター（模擬実験場）として、貧酸素化の進行に伴う栄養環境、赤潮プランクトンの変化や、海底微生物群集の酸素消費活性、生態系のエネルギーフロー等の現状を把握するとともに、海底からの散気（空気の供給）と海藻や底棲動物等の生物機能を活用した栄養塩・有機物の除去を組み合わせた実海域規模の貧酸素環境修復実験に取り組んだ。その成果にもとづき、大村湾の漁業生産を支える生態系の持続性や自律性を回復させる方策について総合的に取りまとめた。それを受けて、本研究で提示した技術の実用化に向けた試験が、26年度から長崎県で実施されることとなった。
日本学術振興会, 基盤研究(A), 長崎大学, 22248022

水産重要魚類稚魚の成育場としての河口・沿岸域生産力の定量評価
科学研究費助成事業
2009年 - 2012年
山下 洋, 笠井 亮秀, 富永 修, 松石 隆, 小路 淳, 富永 修
沿岸域を成育場とするスズキやヒラメなどの有用魚類の生産力を定量的に推定するために、由良川河口・丹後海沿岸域をフィールドとして、海洋物理構造、栄養塩供給、植物プランクトン生産、餌料動物生産、稚魚生産までの生態系を調査した。得られたデータをもとに、デルフト3Dにより基礎生産までの再現性の高いモデル化に成功した。また、エコパスモデルにより稚魚生産までの生態系モデルの基本構造を構築した
日本学術振興会, 基盤研究(B), 京都大学, 21380124

湿地帯におけるメイオベントスの生態学的機能に関する分子生化学的研究
科学研究費助成事業
2009年 - 2011年
豊原 治彦, 笠井 亮秀, 上高原 浩
南西諸島マングローブ湿地帯のセルロース分解機構を調査した結果、底泥のセルラーゼ活性のレベルには違いが認められた。貧毛類、線虫、ヨコエビ、介形虫などのセルロース分解を確認したところ、全てにおいてセルラーゼ活性が検出され、南西諸島マングローブ湿地帯のセルロース分解機構にメイオベントスが関わっていることが明らかになった。また、最も底泥の活性が高かったマーレ川について優占マクロベントスであるキバウミニナTerebralia palustris の底泥中のセルロース分解に対する寄与を調べる為に糞を回収したところ、消化管と糞中のバンドの一部が一致した。以上の結果より南西諸島マングローブ湿地帯底泥のセルロース分解においてベントスの果たす役割が大きいことが明らかになった
日本学術振興会, 基盤研究(B), 京都大学, 21380131

農業系水域ネットワークに生息する生物の移動履歴に関する生体情報分析法の開発
科学研究費助成事業
2008年 - 2010年
東 信行, 佐原 雄二, 笠井 亮秀, 和田 実
1)水路網内の移動履歴追跡
個体標識および個体の分布パターンの季節変化など,従来型の方法を用い,調査地区内の魚類の移動パターンを想定した。その結果,特定の支水路が産卵場である可能性が示唆されたため,採集ポイントを定め支水路と幹線水路での採捕を行ない,サンプルを収集した。今年度は炭素・窒素同位体の分析に加えて、筋肉・肝臓の微量元素分析を行った。28元素を定量化し、生息地ごとの分別が可能であるか判別分析を行った。窒素炭素安定同位体では分別できない場合でも、複数の元素を用いて判別分析を行うことにより、ほぼ100%の分別が可能となった。また,クラスター分析を行うことにより,個体レベルの移動履歴を判別することが可能となった。
2)ため池・ダム湖・その他の環境における分別
1)同様に、水路網につながる、溜池やダム湖内でも生息地点による違いが検出できるかサンプルを収集した。この結果、同位体によって分別できない場合においても、90%以上の分別が可能になり、なおかつその要因が推定できる結果となった。ダム湖では,流入河川毎に異なる特徴が現れ,生息地や出生河川などの判別が可能となることが示された。また同様の手法の発展性について,昆虫や鳥類のサンプルも同時に分析を試みその有用性が示された。
日本学術振興会, 挑戦的萌芽研究, 弘前大学, 20658054

貧酸素化に伴うクラゲの増加が沿岸域の生態系に及ぼす影響の解明
科学研究費助成事業
2006年 - 2008年
笠井 亮秀, 小路 淳, 小路 淳
フィールド調査の結果、ミズクラゲ重量と底層の溶存酸素濃度の間に負の相関が認められた。またミズクラゲの分布域と、魚卵稚仔や動物プランクトンの分布域は、一致していなかった。安定同位体比分析より、ミズクラゲは魚卵稚仔を含む動物プランクトンを主な餌とする雑食性であると推定された。ミズクラゲは強い貧酸素耐性を有しており、沿岸域の 貧酸素化にともない、ミズクラゲへの栄養フローが増大していると推定される。
日本学術振興会, 基盤研究(C), 京都大学, 18580181

強乱流混合海域における力学機構と高生物生産維持機構の解明
科学研究費助成事業
2006年 - 2008年
木村 伸吾, 笠井 亮秀, 森岡 裕詞, シンプソン ジョン, ウィルス フィル, ジョーンズ グゥイン, パウエル ベン
乱流混合が強い英国メナイ海峡域の干潟では、外部からの浮遊性植物プランクトンの流入よりも底生珪藻による基礎生産が大きく、また、底生生物であるヨーロッパイガイ(ムール貝)は底生珪藻と懸濁態有機物を主要な餌資源とする可能性が示された。しかし、ムール貝は懸濁態有機物中のデトライタスを餌として積極的に利用することはなく、その成長には底生珪藻の寄与が極めて大きいことが分かった。結論として、従来考えられてきた外部からの浮遊性植物プランクトンの流入が、高いムール貝の資源量を維持しているのではなく、乱流混合に伴う底生珪藻の生産が重要であることが明らかになった。
日本学術振興会, 基盤研究(B), 東京大学, 18405030

沿岸海域における陸域起源有機物の挙動と資源生物生産に対する役割の解明
科学研究費助成事業
2006年 - 2008年
山下 洋, 上野 正博, 笠井 亮秀, 吉永 郁生, 富永 修
沿岸域における魚介類生産は、陸から河川により輸送される栄養物質で支えられている。森で生産された木の葉や枝などの分解されにくい有機物も、河川に住む水生動物は分解酵素を持つことにより有効に利用した。しかし海では、陸からの有機物を直接利用できる動物は多くなく、陸域起源有機物の多くは微生物などの分解を経て無機化され、植物プランクトンなどから始まる新しい生産系に入ることがわかった。一方、陸域起源有機物からゴカイやアミ類を通してスズキ稚魚やヒラメ稚魚に至る食物網が確認された。
日本学術振興会, 基盤研究(B), 京都大学, 18380115

有明海湾奥部に存続する"大陸沿岸遺存生態系"の起原と特性
科学研究費助成事業
2006年 - 2008年
田中 克, 山下 洋, 笠井 亮秀, 中山 耕至, 白山 義久, 深見 裕伸, 大塚 攻, 原口 昭
有明海特産稚魚の存続に不可欠な筑後川の低塩分高濁度汽水域に存在する大陸沿岸遺存生態系の実体解明を, 主として炭素窒素安定同位体比を用いて試みた. その特異な生態系の起源が現存すると推定された韓国西岸群山に流入する錦江河口域において筑後川と同様の調査を行い, 両者を比較した. いずれの河口域においても河川からの淡水流入量や流入パターンが極めて重要な役割を果たしていることが明らかになった.
日本学術振興会, 基盤研究(A), 京都大学, 18208019

貝類による陸上起源有機物の除去量の推定
科学研究費助成事業
2002年 - 2004年
笠井 亮秀
ヤマトシジミの餌資源を特定することは,資源管理,水質環境保全の両面から意義深いものと考えられる.最近の研究により,河口域に生息するヤマトシジミはあまり餌の選択を行わず,上流側に生息する個体ほど陸上有機物を多く同化することが明らかとなっている.しかし汽水湖に生息するヤマトシジミの食性についてはよく分かっていない.本研究では,小川原湖,十三湖,宍道湖のヤマトシジミの餌資源を安定同位体比分析から推定し,河川棲のヤマトシジミと比較した.
観測は,2004年7月16〜21日,7月21〜25日,8月2〜5日にそれぞれ小川原湖,十三湖,宍道湖で行った.湖内の複数点においてヤマトシジミと水を採取し,水温,塩分,クロロフィル濃度の測定を行った.そして,採取したヤマトシジミ,底生珪藻と水中の粒状有機物について安定同位体比分析を行った.
得られたヤマトシジミの同位体比について判別分析を行った結果,小川原湖,十三湖は大きく2つのグループに分けられ,宍道湖は3つのグループに分けられた.いずれの湖においても,流入河川に近いヤマトシジミほど同位体比が低くなり,特に十三湖ではその傾向が顕著であった.同位体比を基にヤマトシジミの餌資源の寄与率を求めたところ,十三湖の一部を除いて植物プランクトンの寄与率が6割以上と高かった.クロロフィル濃度は,小川原湖と宍道湖では流入河川より明らかに高かったが,十三湖では河川とほぼ同程度であった.また,塩分から河川水の平均滞留時間を見積もると,小川原湖で366日,十三湖で2日,宍道湖で87日となった.すなわち十三湖では,河口域同様水の入れ替わりが速く植物プランクトンの生産がおさえられているため,ヤマトシジミは陸上有機物を多く摂取せざるを得ない.一方,小川原湖や十三湖では水が滞留することで植物プランクトンが豊富に発生し,それがヤマトシジミの餌資源となっていると考えられる.
日本学術振興会, 若手研究(B), 京都大学, 14780413

伊勢湾下層に形成される貧酸素水塊の分布、変動に及ぼす流動構造の影響
科学研究費助成事業
2000年 - 2001年
笠井 亮秀
従来,内湾域において貧酸素水塊は底層に形成されるとされてきたが,中層に形成される場合もある.その違いは,外洋系水の進入深度によって起きる.伊勢湾の場合,外洋系水は伊良湖水道で混合されるので,酸素を豊富に含んでいる.この外洋系水が中層に進入する時は底層に貧酸素水塊ができ,底層に進入する場合は貧酸素水塊が中層に浮き上がる.
進入深度は湾口部と湾内の海水密度の差異によって決まる.そのため,その差異が変化すれば,進入進度も変化する.伊勢湾では,冬季は伊良湖水道の海水の密度が湾内底層の密度よりも大きいので,底層進入となる.一方夏季にはその逆の密度分布となることが多く,外洋系水は中層に進入することが多い.また,伊良湖水道の海水の密度は湾外の海水の影響を受けているため,黒潮の離接岸などによる年毎の変動も大きい.さらに小潮時には伊良湖水道での鉛直混合が若干弱くなるため,下層の密度が湾内底層の密度よりも大きくなり,夏季でも底層進入となる.このような進入進度の変化に伴って,貧酸素水塊も湾奥に押しやられたり中層に浮上したりするなど,短期間のうちに水平・鉛直的な位置が変化する.
伊勢湾の幅は約30kmとロスビーの内部変形半径よりも大きいため,湾内の流れには地球自転の効果が影響を及ぼす.そのため,中層に流入する外洋系水は,愛知県側に押しやられる.その結果三重県側の下層が海水交換から取り残される形になり,低温のドームができ,貧酸素化する.
診断モデルを用いて湾内の密度構造や流れを3次元的に再現するとともに,粒子追跡を行い外洋系水の進入状況を調べた.1986年のように貧酸素化が激しかった年には外洋系水の湾内底層への流入が弱かったため,低温・高密度の海水が湾内下層に広く分布していた.一方比較的酸素濃度が高かった1988年にはエスチュアリー循環によって外洋水が湾内下層に進入し,海水交換が促進される傾向にあった.
日本学術振興会, 奨励研究(A), 京都大学, 12780393

黒潮・親潮移行域の中規模海洋変動が小型浮魚類仔稚魚の成長と生残に及ぼす影響の解明
科学研究費助成事業
1997年 - 1999年
中田 英昭, 笠井 亮秀, 木村 伸吾
本研究では、黒潮・親潮移行域を主な対象として、イワシ類など小型浮魚類の加入量変動に重要なかかわりを持つと考えられる中規模海洋変動、とくに黒潮フロントの擾乱に伴ってその縁辺域に発生する低気圧性の前線渦が、生物生産や仔稚魚の成長・生残に及ぼす影響を明らかにしようとした。1999年度は、これまで黒潮続流フロント域や遠州灘沖の黒潮フロント域で行った前線渦の集中観測の結果を総合的に解析し、黒潮フロントの北縁に広がる黒潮系の暖水城は生物生産が高く、カタクチイワシなどの仔魚の成長や生残に潜在的に好適な条件を提供していること、その生物生産の高さには前線渦の発生に伴う下層の栄養塩の湧昇が多大の貢献をしていることを明らかにした。黒潮系水や親潮系水では、黒潮系暖水域に比ぺて仔魚の成長遠度が遅く、黒潮系水では餌料密度の低さ、親潮系水では低水温がその要困となっているものと推察された。また、このような前線渦近傍の生物生産過程を、生態系の数値モデルによって再現した。
一方、前線渦付近の流れは、仔稚魚を渦域に水平的に引き込みその縁辺に集積させる働きをしており、栄養塩の湧昇による餌料生物生産の増大と相まって、このような前線渦との遭遇の有無が、黒潮および黒潮続流域における仔稚魚の生き残りの程度を大きく左右している可能性があることを指摘した。
以上の研究成果を、lCES J.Mar.Sci.などに論文として印刷公表するとともに、日本水産学会、日本海洋学会、水産海洋学会や、いくつかの国際シンポジウムで口頭発表を行った。
日本学術振興会, 基盤研究(B), 東京大学, 09460085

遠隔測定による水圏生物の環境履歴と回遊経路の解析に関する研究
科学研究費助成事業
1996年 - 1997年
坂本 亘, 笠井 亮秀, 荒井 修亮
平成8年度には,耳石などを中心とした硬組織内微量元素分析による,個体が経験した環境履歴について解析を行った.また,平成9年度にはこの実験と平行して,マイクロデータロガによる魚類の行動解析も試みた.最初の段階では,耳石に含まれる微量元素濃度のレベルが分からなかったため,高性能の分析機(PIXE)を用いた.その結果,環境履歴を解析する際に注目する元素として,Srが適当であるとの結果を得た.さらに遠隔測定解析の対象魚種としては,淡水域と海水域とで生活を行うサケ・マス類がSr濃度変化に急激な相違が見い出せるため,適当であることがわかった.サクラマスについて,いつ淡水域から海洋にくだり,さらに再び産卵のため河川にもどるのかを推定することが可能となった.実験は平成8年度には富山県神通川で,また平成9年度には神通川と同時に,青森県老部川に帰る個体についても解析を試みた.これらの研究と同時に,サケ・マスが母川を識別する機構を推定するため,水深と水温が記録できるマイクロデータロガを,母川回帰したサクラマスに装着し,再度海洋に放流して回収を試みたが失敗した.マイクロデータロガによる魚類の回遊行動は,イナダを用いて行った実験により成功した.これは10月から11月に相模湾で行ったものであり,総計12個体から2ないし3カ月間,5分あるいは20分間隔で資料を得ることができた.これらの成果はいくつかの学会誌に報告し,成果の一部として取りまとめた.また,平成9年度には安定同位体比による,食物連鎖網を通してみた環境履歴解析も試み,個体群識別が可能なことを見い出した.これらの研究は新たな展開が期待されるため,さらに平成10年度科学研究費として申請を行った.
日本学術振興会, 基盤研究(B), 京都大学, 08456097

黒潮フロント域における低次生産に関する研究
科学研究費助成事業
1996年 - 1996年
笠井 亮秀
黒潮フロントの蛇行に伴う低気圧性渦は,マイワシ等の稚仔魚あるいはその餌生物の輸送や分布に大きな影響を及ぼしている.また黒潮フロント付近は生産性が高く稚仔魚にとって好都合な海域となっている.しかしこの低気圧性渦に対応した生物の分布・動態・生産性については,よく理解されていない.そこで本研究では,1994年5月に遠州灘の黒潮前線域における低気圧性渦を観測した結果に基づいて数値実験を行い,現実の海洋でどの様に植物プランクトン量,栄養塩濃度,水温運動エネルギーが変化するかを再現し,渦の発生に伴う生物生産量を定量的に堆定した.
生物の基礎方程式は,クロロフィル濃度,植物プランクトン内窒素濃度,海水中の硝酸・亜硝酸態窒素濃度から構成されている.また物理モデルとしては,鉛直一次元モデルを用い,鉛直拡散係数はturbulent closureモデルから決定した.
モデルを用いて数値実験を行った結果,渦が形成されてから4日目にそれまで一定であった植物プランクトンの増殖率が急速に減少すると共に,植物プランクトン内窒素濃度が最大となった.そして5日目にはクロロフィル濃度が最大値に達した.これらの結果は観測から求められた結果とよく一致している.また,海水中の硝酸態窒素濃度が1μM以下になった時点で増殖率は急速に低下したが,クロロフィル濃度はそれとは時間差を持って急速に減少していることから,植物プランクトンが二次生産を伴う捕食や沈降にまわされていることが分かった.またこれらの時間スケールとして渦が生成されてから約1週間弱で1サイクルの現象が終了することも,観測結果と一致する.この低気圧性渦による基礎生産量を見積もると,40gCm^<-2>y^<-1>となり,過去に推定された遠州灘沿岸域全体での基礎生産量の20〜30%を担っていると考えられる.
日本学術振興会, 奨励研究(A), 京都大学, 08760179

生物装着記録標識を用いたブリ成魚の回遊行動に関する研究
科学研究費助成事業
1995年 - 1995年
笠井 亮秀
海洋環境の変動に対応したブリの回遊行動を調べる実験を,1995年10月から11月にかけて相模湾において行った.
1.超小型超音波発信器(ピンガー)を用いた実験
まず,1日以下のブリの行動を詳細に探るために,ピンガーをブリの背中に取り付け,約20時間追跡した.用いたピンガーは水温と水深を測定し,3秒間隔でデータ値を発信するもので,船上の受信器でデータをキャッチできる仕組みになっている.船の位置をGPSシステムにより確定することにより,水平移動も同時に調査できる.
水平行動に関しては,放流直後ブリは約1時間迷走したが,その後瀬に付き,昼間はその瀬の周辺に留まっていた.日没後急に移動し始め,約5km離れた人工漁礁に付いた後は夜明けまで漁礁から離れなかった.鉛直移動については,昼間は20〜40m深を活発に遊泳し,索餌を試みていたようである.ところが,夜間はほとんどの時間を10m以浅で過ごしていた.その一方で,1時間に数回約40mにまで達する急激なダイビングを見せた.
2.メモリー内蔵型超小型測定器(データロガー)を用いた実験
数日以上の長期にわたるブリの行動を調べるために,データロガーをブリの背中に取り付け,放流した.用いたデータロガーは水温または水深を測定し,20分毎にメモリーにデータを保存できるもので,回収後そのデータをパーソナルコンピュータにより解析できる.放流した3尾のうち,2個体が約2週間後に相模湾内で再捕された.
約2週間のうち2度ほど低気圧の通過により水温が急激に降下したが,それに伴ってブリは平均遊泳水深を変化させたり,鉛直移動の回数を変化させるなど,その行動は明らかに変化した.一方,潮汐や降雨に伴う塩分や濁度の変化に対しては特別な行動の変化は見られなかった.また,ピンガーを用いた実験同様,昼間活発に行動し夜は海表面近くで留まるという明らかな昼行性が確認できた.
日本学術振興会, 奨励研究(A), 京都大学, 07760184

遊泳行動記録標識による産卵期アカウミガメの回遊機構解析に関する研究
科学研究費助成事業
1993年 - 1994年
坂本 亘, 笠井 亮秀, 荒井 修亮, 藤原 建紀
本研究は、海域実験と室内実験とに分けて行われた.海域実験としては,
1)人工衛生を利用した回避経路の測定,2)記録標識による産卵期間中の摂餌生態,3)潜水行動の解析,4)記録標識を用いた日本列島の主要産卵場における砂中温度の同時測定,5)来遊頭数の年別比較が試みられた.室内実験では,6)砂中温度変化にともなう孵化仔ガメ雌雄比,7)砂中温度と仔ガメ孵化率との関係調査が行われたが,これらの中には一部海域実験の結果が取り込まれた.以下に各実験項目ごとに実績を報告する.
1)人工衛生アルゴスシステムを用いて,2頭にPTTを装着した.受信さた位置情報は,7点でありすべてが産卵場沖合いからであった.このことから見て,2個体は広範囲な回遊は行っていなかったことが判明した.
2)胃内に挿入した温度記録標識により,産卵期アカウミガメの摂餌の有無について15個体測定した結果,摂餌はしていないことが判明した.
3)潜水記録には、極力エネルギーを節約するように,一定の水深のみが記録され,呼吸時以外は静止していることを示唆した.しかし,環境水温が低い時には,表層回遊に切り替わることが,深度と水温記録から判明した.
4)来遊頭数は5年間減少し続けた.全国の砂中温度環境は集計整理中である.
5)卵の孵化では,発育段階23から26の期間に経験する温度によって,雌雄が決定されることが判明した.これは,この期間に分泌されるホルモン量が温度に依存することによると思われ,現在新しい研究を計画中である.
6)実測された砂中温度により,実際の砂浜での雌雄比の時間変化を計算した.6月中旬以前と8月中旬以後の産卵個体は雄となることが判明した.
7)孵化率は砂中温度に関係なく,70%で積算温度一定の法則が見いだされた.
日本学術振興会, 一般研究(B), 京都大学, 05454093

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The effect of egg and larval transport on the recruitment of Pelagic fish　　　　　　　　　　　　　　　
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