中村 剛 (ナカムラ コウ)
| 北方生物圏フィールド科学センター 耕地圏ステーション 植物園 | 准教授 |
Last Updated :2025/04/25
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- 70532927
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- Morphological and molecular data demonstrate the existence of Betula fruticosa and B. middendorffii and the absence of B. ovalifolia in the northeastern China
Xingjian Dun, Junyi Ding, Katarzyna A. Jadwiszczak, Shaojie Zhou, Qi Wang, Koh Nakamura, Yuyun Fan, Teruyoshi Nagamitsu, Dafeng Chen, Nian Wang
Botanical Journal of the Linnean Society, 00, 1, 10, 2024年11月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - Ecological and evolutionary factors contribute to the uneven diversification of firs in the Northern Hemisphere
Qiaoping Xiang, Jie Yang, Davids S. Gernand, Ling Yang, Jia-Ming Pan, Ruichen Xiang, Cheng Zhao, Koh Nakamura, Xianchun Zhang, Yongbo Liu, Ran Wei
Journal of Biogeography, 0, 1, 15, 2024年11月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - The complete chloroplast genome sequence of Hypecoum erectum L. (Papaveraceae)
Haruto Mano, Eugeny V. Boltenkov, Elena A. Marchuk, Koh Nakamura, Watanabe Yoichi
Mitochondrial DNA Part B, 9, 8, 1010, 1014, 2024年08月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - Phenolic compounds from five alpine Leontopodium species (Asteraceae) in Japan
Yoshinori Murai, Hayato Tsuboi, Koh Nakamura
Bulletin National Museum of Nature and Science, Series B, Botany, 50, 2, 79, 86, 2024年05月, ［査読有り］, ［最終著者］, ［国内誌］
英語, 研究論文（学術雑誌） - Circular genetic structure of the Abies nephrolepis species complex shaped by the circular landform of Northeast Asia
Myounghai Kwak, Koh Nakamura, Qiaoping Xiang, Jun Wen, Eun Sil Lee, Jaram Hong, Nataliya Kovtonyuk, Maria Kryukova, Olga Korchagina, Kyung Ah Koo, Masakazu G. Iwaizumi, Byoung Yoon Lee, Goro Kokubugata, Xianchun Zhang, Dmitry Lebedev
Journal of Biogeography, 51, 1533, 1548, 2024年03月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - 『北海道主要樹木圖譜』成立過程の再検討
加藤克, 中村剛
札幌博物場研究会誌, 1, 27, 2023年11月, ［査読有り］, ［国内誌］
日本語, 研究論文（学術雑誌） - Ghost introgression facilitates genomic divergence of a sympatric cryptic lineage in Cycas revoluta
Jui-Tse Chang, Koh Nakamura, Chien-Ti Chao, Min-Xin Luo, Pei-Chun Liao
Ecology and Evolution, 13, 8, e10435, 2023年08月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - 北海道大学植物園におけるヒダカソウCallianthemum miyabeanum生息域外保全の10年間（2011～2020年）の成果
永谷工, 稲川博紀, 高田純子, 西川洋子, 島村崇志, 陶山佳久, 水永優紀, 中村 剛
日本植物園協会誌, 57, 57, 66, 71, 日本植物園協会, 2023年03月, ［査読有り］, ［最終著者］, ［国内誌］
日本語, 研究論文（学術雑誌） - Phylogenetic relationships among populations of Portulaca okinawensis (Portulacaceae) in the Ryukyu Archipelago of Japan using MIG-seq SNP data
Goro Kokubugata, Satoshi Kakishima, Atsushi Abe, Koh Nakamura, Kuo-Fang Chung, Masatsugu Yokota
Bulletin National Museum of Nature and Science, Series B, Botany, 49, 33, 40, 2023年02月, ［査読有り］, ［国際共著］, ［国内誌］
英語, 研究論文（学術雑誌） - Phenolic compounds from Hedysarum vicioides (Fabaceae) in Japan
Yoshinori Murai, Koh Nakamura
Bulletin National Museum of Nature and Science, Series B, Botany, 48, 95, 100, 2022年08月, ［査読有り］, ［最終著者］, ［国内誌］
英語, 研究論文（学術雑誌） - Divergence with Gene Flow and contrasting population size blur the species boundary in Cycas sect. Asiorientales, as inferred from morphology and RAD-seq data
Jui-Tse Chang, Chien-Ti Chao, Koh Nakamura, Hsiao-Lei Liu, Min-Xin Luo, Pei-Chun Liao
Frontiers in Plant Science, 13, Article 824158, 2022年05月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - Lectotypification of Saxifraga yuparensis Nosaka (Saxifragaceae)
SAYA TAMURA, HIDEKI TAKAHASHI, KOHTAROH SHUTOH, KEN SATO, HIROYUKI SATO, TAKASHI SHIMAMURA, KOH NAKAMURA
Phytotaxa, 543, 1, 095, 098, Magnolia Press, 2022年04月08日, ［査読有り］, ［最終著者, 責任著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, We discussed the typification of the name Saxifraga yuparensis applicable to an alpine plant described from Hokkaido, northern Japan. The holotype is not found in the cited herbarium and other herbaria related to Nosaka. No isotypes or paratypes were cited in its protologue, and no other uncited specimens are unambiguously deduced to have been used by Nosaka when describing the species and do not comprise the original material (Art. 9.4 of the Botanical Code). Therefore, an illustration (Fig. 11 in Nosaka 1974) published as part of the protologue is the only original material that can be chosen in lectotype designation in conformity with Art. 9.12. The illustration is here designated as the lectotype for the name. - Chromosome number of Micranthes nelsoniana var. tateyamensis (Saxifragaceae)
Tomoko Fukuda, Toshifumi Adachi, Koh Nakamura
Journal of Japanese Botany, 97, 241, 245, 2022年04月, ［査読有り］, ［最終著者］, ［国内誌］
英語 - 北大植物園におけるクマゲラDryocopus martius martius (Linnaeus, 1758)の初記録
髙谷文仁, 中村剛, 冨士田裕子
北大植物園技術報告・年次報告, 2022年03月, ［国内誌］
日本語, 研究論文（大学，研究機関等紀要） - 北大植物園から札幌市円山動物園への伐木及び剪定枝の提供－アジアゾウ Elephas maximus Linnaeus, 1758の飼料として－
髙谷文仁, 高田純子, 朝倉卓也, 野村友美, 中村剛, 冨士田裕子
北大植物園技術報告・年次報告, 2022年03月, ［国内誌］
日本語, 研究論文（大学，研究機関等紀要） - Inferring the migration of Lobelia sect. Hypsela (Campanulaceae) from the Ryukyu Archipelago of Japan to mainland China using molecular data
Goro Kokubugata, Koh Nakamura, Yumiko Hirayama, Masatsugu Yokota
Bulletin National Museum of Nature and Science, Series B, Botany, 48, 23, 29, 2022年02月, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌） - Parentage of hybrids in a disturbed population of Betula ovalifolia endangered in Japan and suggestions for conservation management
Yuki Shiotani, Tomoko Fukuda, Elena A. Marchuk, Pavel V. Krestov, Ekaterina A. Petrunenko, Svetlana N. Bondarchuk, Yoko Nishikawa, Takashi Shimamura, Yoshiyasu Fujimura, Koh Nakamura
Acta Phytotaxonomica et Geobotanica, 73, 119, 130, 2022年02月, ［査読有り］, ［最終著者, 責任著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - 北海道大学植物園が絶滅危惧植物保全に果たす役割とその課題
中村剛, 田村紗彩
北方林業, 73, 11, 15, 2022年01月, ［招待有り］, ［筆頭著者, 責任著者］, ［国内誌］
日本語, 研究論文（学術雑誌） - A discussion of the relationship between Ophiorrhiza exigua and O. michelloides (Rubiaceae) with the description of a new species
Yan‐Hua Hu, Wen‐Jian Liu, Xiao‐Fei Song, Guo‐Xing Deng, Koh Nakamura, Lei Wu, Quan‐Ru Liu
Nordic Journal of Botany, 39, 6, e03138, Wiley, 2021年06月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - Begonia ×dinglensis, a natural hybrid of Philippine Begonia section Baryandra, as evidenced by morphological, phylogenetic and cytological data
KOH NAKAMURA, ROSARIO RIVERA RUBITE, YOSHIKO KONO, JELENE V. MACABASCO, ARLENE D. TALAÑA, GRECEBIO J. D. ALEJANDRO, MENG-JUNG HO, HSUN-AN YANG, KUO-FANG CHUNG, CHING-I PENG
Phytotaxa, 494, 1, 151, 164, Magnolia Press, 2021年03月31日, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Here we describe a natural hybrid of Philippine Begonia from Bulabog Puti-an National Park in Dingle, Iloilo, Panay Island. The hybrid, named Begonia ×dinglensis after its place of origin, grows on moist, coralline-rock slopes at 200 m elevation. Morphological, molecular, and cytological evidence support its origin from natural hybridization between Philippine endemics B. camiguinensis and B. nigritarum. - Phylogenetic position of Lagotis kunawurensis (Plantaginaceae) in Himalaya, a species of boreal L. glauca aggregate.
Koh Nakamura, Rinchen Yangzom, Atsushi Sugano, Yoshinori Murai
Bulletin National Museum of Nature and Science, Series B, Botany, 47, 1, 6, 2021年03月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国内誌］
英語 - Merger of Betula tatewakiana (Betulaceae) from northern Japan with northeast Asian B. ovalifolia based on ploidy level
Y. Shiotania, T. Fukuda, E.A. Marchuk, E.A. Petrunenko, P.V. Krestov, S.N. Bondarchuk, Y. Nishikawa, T. Shimamura, Y. Fujimura, K. Nakamura
Phytokeys, 170, 83, 91, 2020年12月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, 31624334 - New localities of seed plants recorded from the subtropical Ryukyus of Japan at island level based on herbarium data
Goro Kokubugata, Atsushi Ebihara, Yukiko Saito, Masaharu Amano, Atsushi Abe, Koh Nakamura, Akiyo Naiki, Tadashi Kajita, Masatsugu Yokota
Bulletin National Museum of Nature and Science, Series B, Botany, 46, 75, 87, 2020年05月, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌） - The first record of the genus Vitex (Lamiaceae) from Hokkaido, northern Japan
Koh Nakamura, Goro Kokubugata, Ken Sato
Journal of Phytogeography and Taxonomy, 68, 2020年, ［査読有り］, ［筆頭著者］, ［国内誌］
研究論文（学術雑誌） - Two-way migration of Lychnis wilfordii caused by the circular landform of Japan-Korea-northeast China-Russian Far East region and its suggestion for conservation in northeast Asia
Saya Tamura, Myounghai Kwak, Goro Kokubugata, Chan-ho Park, Byoung-Yoon Lee, Tomoko Fukuda, Ekaterina Petrunenko, Inna Koksheeva, Elena Pimenova, Pavel Krestov, Svetlana Bondarchuk, Jin-Shuang Ma, Hai-Cheng Zhou, Hayato Tsuboi, Yoko Nishikawa, Takashi Shimamura, Hiroko Fujita, Koh Nakamura
Cold Spring Harbor Laboratory, 2019年11月27日
ABSTRACT
In northeast Asia, substantial portion of the floras, including endangered species, are shared among its component countries in the continental, peninsula, and island parts largely through Quaternary migration. To effectively conserve nationally endangered plants in Northeast Asia, transnational conservation studies are vitally needed. Lychnis wilfordii (Caryophyllaceae) has disjunct distribution in Russian Far East (Primorsky Krai), northeast China (Jilin), Korea (Gangwon-do) and Japan (Hokkaido, Aomori, Nagano), surrounding the sea, and this is designated as an endangered species in Japan and Korea. Population genetic and molecular dating analyses were conducted 1) to elucidate geographic genetic structure covering the species range, 2) to test possible scenarios of migration, and 3) to develop logical plans for effective conservation. Population genetic analyses indicated the continent and peninsula parts (north and south Primorsky Krai, Jilin, and Gangwon-do) had higher genetic diversity compared to those in the Japanese Archipelago (Hokkaido and Nagano). Five genetically distinct groups were recognized, namely, Nagano, Gangwon-do, Jilin, north and south Primorsky Krai plus Aomori, and Hokkaido. Genetic distance between Hokkaido and Nagano was larger than between Hokkaido and north Primorsky Krai, and between Nagano and Gangwon-do, crossing national borders and the natural barrier of the sea. Considering these results, L. wilfordii likely migrated from the Asian continent to the Japanese Archipelago using two routes: north route from Russian Far East to Hokkaido and Aomori, and south route from the Korean Peninsula to Nagano. Based on molecular dating, migration from the continent to the islands likely occurred from the middle Pleistocene to the Holocene. For effective conservation of L. wilfordii, Hokkaido and Nagano populations should be distinguished as different evolutionary significant units, although these two regions belong to the same country, because Hokkaido and Nagano populations are at the different ends of the two migratory routes based on the migration scenario. - Genetic diversity and population structure of Lychnis wilfordii (Caryophyllaceae) with newly developed 17 microsatellite markers
Bora Kim, Koh Nakamura, Saya Tamura, Byoung Yoon Lee, Myounghai Kwak
Genes & Genomics, 2019年, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - 崕山固有の希少植物キリギシソウの保全における今後の課題：基亜種カラフトミヤマイチゲにみる健全な生育環境と，キリギシソウの生息域外保全
中村剛
崕山自然保護20周年記念誌, 26, 33, 2019年, ［招待有り］
日本語, 研究論文（その他学術会議資料等） - 北海道大学植物園ウェブサイトのリニューアル：一般向けサイト，研究・教育者向けサイト，英語サイトの作成
板羽貴史, 高田純子, 中村剛, 加藤克
北大植物園技術報告･年次報告, 17, 2, 4, 2019年
日本語, 研究論文（大学，研究機関等紀要） - Reappraisal of Tashiroea as a genus independent of Bredia (Melastomataceae) based on molecular data
Goro Kokubugata, Koh Nakamura, Wen-Hsi Kuo, Zhe-Chen Qi, Kuo-Fang Chung, Cheng-Xin Fu, Yumiko Suzuki, Masatsugu Yokota
Phytotaxa, 392, 75, 83, 2019年, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌） - Molecular and cytological evidences denied the immediate-hybrid hypothesis for saxifraga yuparensis (Sect. bronchiales, saxifragaceae) endemic to mt. yubari in Hokkaido, northern Japan
Saya Tamura, Tomoko Fukuda, Elena A. Pimenova, Ekaterina A. Petrunenko, Pavel V. Krestov, Svetlana N. Bondarchuk, Olga A. Chernyagina, Yoshihisa Suyama, Yoshihiro Tsunamoto, Ayumu Matsuo, Hayato Tsuboi, Hideki Takahashi, K. E.N. Sato, Yoko Nishikawa, Takashi Shimamura, Hiroko Fujita, K. O.H. Nakamura
Phytotaxa, 373, 1, 53, 70, 2018年10月23日, ［査読有り］, ［責任著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, © 2018 Magnolia Press. An alpine plant Saxifraga yuparensis is endemic to a scree consisting of greenschist of Mt. Yubari in Hokkaido, Japan and it has been proposed as an immediate hybrid derived from two species of the same section Bronchiales based on morphological intermediacy: namely S. nishidae, a diploid species endemic to a nearby cliff composed of greenschist and tetraploid S. rebunshirensis comparatively broadly distributed in Japan and Russian Far East. Saxifraga yuparensis is red-listed and it is crucial for conservation planning to clarify whether this is an immediate hybrid and lacks a unique gene pool. The immediate-hybrid hypothesis was tested by molecular and cytological data. In nuclear ribosomal and chloroplast DNA trees based on maximum parsimony and Bayesian criteria, S. yuparensis and S. rebunshirensis formed a clade with several other congeners while S. nishidae formed another distinct clade. Genome-wide SNP data clearly separated these three species in principal coordinate space, placing S. yuparensis not in-between of S. rebunshirensis and S. nishidae. Chromosome observation indicated that S. yuparensis is tetraploid, not triploid directly derived from diploid-tetraploid crossing. Additionally, observation of herbarium specimens revealed that leaf apex shape of S. yuparensis fell within the variation of S. rebunshirensis. These results indicate that S. yuparensis is not an immediate hybrid of S. rebunshirensis and S. nishidae but a distinct lineage and an extremely narrow endemic species, that deserves for intensive conservation. - 植物園と地権者企業、行政が協力した絶滅危惧植物の植え戻し計画と実施の検討：北海道におけるエンビセンノウ（ナデシコ科）の事例
田村紗彩, 冨士田裕子, 西川洋子, 島村崇志, 稲川博紀, 高田純子, 永谷工, 中村剛
日本植物園協会誌, 53, 27, 34, 2018年, ［査読有り］
日本語 - 北海道指定希少野生植物エンビセンノウ（ナデシコ科）の北大植物園における生態展示の実施
田村紗彩, 高田純子, 稲川博紀, 永谷工, 冨士田裕子, 中村剛
北大植物園技術報告･年次報告, 16, 11, 17, 2018年, ［査読有り］
日本語, 研究論文（大学，研究機関等紀要） - Flavonoids from three Wild Glycine Species in Japan and Taiwan
Tsukasa Iwashina, Goro Kokubugata, Koh Nakamura, Takayuki Mizuno, Hari Prasad Devkota, Masatsugu Yokota, Yoshinori Murai, Yukiko Saito
Natural Product Communications, 13, 12, 1641, 1644, 2018年, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Fourteen flavonols, four flavones and six isoflavones were isolated from the aerial parts of two Japanese Glycine species, G. tabacina and G. koidzumii, and the leaves of Taiwanese G. max subsp. formosana. Of their flavonoids, twelve flavonols were identified as kaempferol 3-O-sophoroside (1), 3-O-rutinoside (2), 3-O-robinobioside (3) and 3-O-rhamnosyl-(1→4)-[rhamnosyl-(1→6)-galactoside] (4), quercetin 3-O-gentiobioside (5), 3-O-glucoside (6), 3-O-galactoside (7), 3-O-rutinoside (8), 3-O-robinobioside (9) and 3-O-rhamnosyl-(1→4)-[rhamnosyl-(1→6)-galactoside] (10), and isorhamnetin 3-O-rutinoside (11) and 3-O-robinobioside (12). Other two flavonols were characterized as isorhamnetin 3-O-rhamnosylrhamnosylglucoside (13) and 3-O-rhamnosylrhamnosylgalactoside (14). Four flavones and six isoflavones were estimated as schaftoside (15), apigenin 6,8-di-C-arabinoside (16), luteolin 7-O-glucoside (17) and chrysoeriol 7-O-glucoside (18), and daidzein 7-O-glucoside (19), 4´-O-glucoside (20) and 7-O-xylosylglucoside (21), genistein 7-O-glucoside (22) and 4´-O-glucoside (23), and 3´-O-methylorobol 7-O-glucoside (24). Although flavonoid composition of G. tabacina and G. koidzumii was similar to each other, that of G. max subsp. formosana was different with those of two Japanese Glycine species described above. Flavonoids of their Glycine species were reported for the first time except for those of G. tabacina. - Unique parallel radiations of high-mountainous species of the genus Sedum (Crassulaceae) on the continental island of Taiwan
Takuro Ito, Chih-Chieh Yu, Koh Nakamura, Kuo-Fang Chung, Qin-er Yang, Cheng-Xin Fu, Zhe-Chen Qi, Goro Kokubugata
MOLECULAR PHYLOGENETICS AND EVOLUTION, 113, 9, 22, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017年08月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, We explored the temporal and spatial diversification of the plant genus Sedum L. (Crassulaceae) in Taiwan based on molecular analysis of nrITS and cpDNA sequences from East Asian Sedum members. Our phylogenetic and ancestral area reconstruction analysis showed that Taiwanese Sedum comprised two lineages that independently migrated from Japan and Eastern China. Furthermore, the genetic distances among species in these two clades were smaller than those of other East Asian Sedum clades, and the Taiwanese members of each Glade occupy extremely varied habitats with similar niches in high-mountain regions. These data indicate that species diversification occurred in parallel in the two Taiwanese Sedum lineages, and that these parallel radiations could have occurred within the small continental island of Taiwan. Moreover, the estimated time of divergence for Taiwanese Sedum indicates that the two radiations might have been correlated to the formation of mountains in Taiwan during the early Pleistocene. We suggest that these parallel radiations may be attributable to the geographical dynamics of Taiwan and specific biological features of Sedum that allow them to adapt to new ecological niches. (C) 2017 Elsevier Inc. All rights reserved. - 北海道指定希少野生植物エンビセンノウ（ナデシコ科）の保全を目的とした生態調査と遺伝解析
田村 紗彩, 冨士田 裕, 西川 洋子, 島村 崇志, 稲川 博紀, 高田 純子, 中村 剛
日本植物園協会誌, 51, 51, 33, 43, 日本植物園協会, 2016年, ［査読有り］
日本語 - Characterization of transcriptome and development of novel EST-SSR makers based on next-generation sequencing technology in Neolitsea sericea (Lauraceae) endemic to East Asian land-bridge islands
Lu-Yao Chen, Ya-Nan Cao, Na Yuan, Koh Nakamura, Guo-Ming Wang, Ying-Xiong Qiu
MOLECULAR BREEDING, 35, 9, e187, SPRINGER, 2015年09月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Neolitsea sericea (Lauraceae), endemic to East Asian land-bridge islands, is an economically important tree species because of its characteristic aroma and timber uses. However, due to the lack of efficient molecular markers, the genetic diversity and historical demography of this endemic species is not clearly understood. In this study, we performed high-throughput transcriptome sequencing of N. sericea leaves using the Illumina HiSeq (TM) 2000 sequencing platform, and generated large transcript sequences for functional characterization and development of gene-associated SSR markers. A total of 68,624 unigenes (mean length 733 bp) were assembled from about 54.7 million reads, and 41,130 (59.94 %) unigenes of all the assembled unigenes showed similarity to public databases. From 68,624 unigenes, 13,213 expressed sequence tag-simple sequence repeats (EST-SSRs) were identified. Di-nucleotide SSRs were the most abundant motif (36.5 %), followed bymono-(32.3 %) and tri-nucleotide (26.5 %) repeats. From the 13,213 EST-SSRs, 1191 primer pairs were designed for marker mining. After selecting 131 of these pairs at random for further validation, 13 polymorphic pairs were identified as polymorphic SSR loci. These 13 EST-SSR markers showed intermediate levels of genetic diversity (e.g., N-A = 7.15; mean H-E = 0.51) when surveyed across six populations from East China (2), Taiwan (1), Korea (1), and the Ryukyus (1) and Honshu (1) of Japan. Both genetic distance and STRUCTURE analyses identified two genetic clusters largely congruent with recent findings revealed by variations in chloroplast DNA sequences and genomic SSRs. The EST-SSR markers developed in our research will be an information resource for future studies on ecological, evolutionary, and conservation genomics in N. sericea. - Taxonomic reexamination of Portulaca boninensis (Portulacaceae) in the Bonin (Ogasawara) Islands of Japan using molecular and morphological data
Goro Kokubugata, Hidetoshi Kato, Duilio Iamonico, Hana Umemoto, Takuro Ito, Koh Nakamura, Noriaki Murakami, Masatsugu Yokota
PHYTOTAXA, 217, 3, 279, 287, MAGNOLIA PRESS, 2015年06月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Molecular phylogenetic analyses, morphological observations, and nomenclatural studies were carried out to investigate the taxonomic status of Portulaca boninensis, endemic species from the Bonin (Ogasawara) Islands (Japan). The results addressed controversy between the widely naturalized P. boninensis and P. pilosa, indicating that they are phylogenetically and morphologically distinct. Furthermore, P. boninensis was showed to be conspecific to P. psammotropha which is until now recorded in southern China, Taiwan, and the northern Philippines, but not in the Ryukyus. The name of P. psammotropha was lectotypified on a specimen preserved at K. Based on phylogeography, P. psammotropha likely migrated to the Oceanic Bonin Islands oversea by sea-current dispersal or by exo-and/or end ozoochory through migratory birds without passing through Ryukyus. - Distyly and floral morphology of Psychotria cephalophora (Rubiaceae) on the oceanic Lanyu (Orchid) Island, Taiwan
Kenta Watanabe, T. Y. Aleck Yang, Chihiro Nishihara, Tai-Liang Huang, Koh Nakamura, Ching-I Peng, Takashi Sugawara
BOTANICAL STUDIES, 56, e10, SPRINGER, 2015年05月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Background: Psychotria cephalophora Merr. (Rubiaceae), a shrub in oceanic islands of Taiwan and the Philippines, appears to be distylous, but distyly is usually rare on oceanic islands. To elucidate the functional breeding system of P. cephalophora can improve our understanding of plant reproductive ecology on oceanic islands.
Results: Field investigations on Lanyu (Orchid Island) off the coast of southeastern Taiwan revealed the flowers to be distylous with short (S)-and long (L)-styled morphs, with only one morph per individual. Laboratory observations revealed that both morphs had stainable pollen grains and indicated dimorphism in stigmatic papillae and pollen size. In hand pollination experiments, the pollen tubes reached the base of the style in intermorph crossing, whereas they rarely penetrated stylar tissue in intramorph crossing and selfing. Open pollinated S- and L-styled flowers produced fruit.
Conclusions: The results indicate that the breeding system of P. cephalophora is morphologically and functionally distylous. - Phylogeny and biogeography of the Viola iwagawae-tashiroi species complex (Violaceae, section Plagiostigma) endemic to the Ryukyu Archipelago, Japan
Koh Nakamura, Tetsuo Denda, Goro Kokubugata, Chiun-Jr Huang, Ching-I Peng, Masatsugu Yokota
PLANT SYSTEMATICS AND EVOLUTION, 301, 1, 337, 351, SPRINGER WIEN, 2015年01月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, In continental island biogeography, geologically age-old straits have been considered to be the most likely barriers in determining geographical patterns of speciation/genetic differentiation among islands. Straits with similar ages may have had different influences if geographical width across the straits had fluctuated differently in the past. However, this issue has seldom been discussed. We explored it by studying the Viola iwagawaetashiroi species complex in the Ryukyu Archipelago, Japan. The archipelago is divided into three island groups, the northern, central, and southern Ryukyus, by two old straits called the Tokara and Kerama gaps, which are roughly of the same age. Bayesian and maximum parsimony phylogenetic analyses of section Plagiostigma and Bayesian molecular dating using multiple calibration points were conducted based on nuclear ribosomal DNA internal transcribed spacer (ITS) sequences. To reveal the detailed genealogy of the species complex, statistical parsimony networks were estimated separately for the ITS and chloroplast DNA (cpDNA) sequences and a calibrated multispecies coalescent tree based on both ITS and cpDNA sequences was constructed. Results suggest that the V. iwagawae-tashiroi species complex originated in the Ryukyu Archipelago when this region formed part of the East Asian continental margin or was formed by a few larger islands and that the complex was already distributed across the archipelago by the late Pliocene-early Pleistocene. Divergence time estimations suggest that the Kerama Gap has been a long-term effective barrier to dispersal preventing gene flow across it, whereas recent dispersal over the Tokara Gap was inferred. These contrasting results are likely explained by the fact that the minimum geographical width across the straits is geohistorically and currently greater over the Kerama Gap than at the Tokara Gap. - New distribution record of Begonia grandis (Begoniaceae, section Diploclinium) from Taiwan, with subspecies assignment based on morphology and molecular phylogeny
Koh Nakamura, Yi-Fu Wang, Meng-Jung Ho, Kuo-Fang Chung, Ching-I Peng
Taiwania, 60, 49, 53, 2015年, ［査読有り］, ［国際共著］, ［国際誌］ - Ixeridium calcicola (Compositae), a New Limestone Endemic from Taiwan, with Notes on Its Atypical Basic Chromosome Number, Phylogenetic Affinities, and a Limestone Refugium Hypothesis
Koh Nakamura, Shih-Wen Chung, Yoshiko Kono, Meng-Jung Ho, Tian-Chuan Hsu, Ching-I Peng
PLOS ONE, 9, 10, e109797, PUBLIC LIBRARY SCIENCE, 2014年10月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, A new species Ixeridium calcicola (Compositae) endemic to middle altitude (ca 1,000-2,000 m asl) limestone mountains of eastcentral Taiwan is described based on morphological and chromosome cytological observations and molecular phylogenetic analyses. Ixeridium calcicola resembles Ixeridium transnokoense, endemic to upper montane and alpine ranges (2,600-3,500 m asl) of Taiwan, in the dwarf habit, but differs in the oblong to lanceolate leaf blades (vs. linear to linear-lanceolate), the presence of mucronulate teeth on the leaf margin and petiole (vs. smooth to very sparse), the dark purple lower leaf surface (vs. greenish), the capitulum with 10 to 12 florets (vs. 5 to 7) and 8 to 10 inner phyllaries (vs. 5, rarely to 7). The basic chromosome number in Ixeridium was known as X = 7. However, the new species has a basic chromosome number of X = 8, as recorded also in the closely related Ixeris. Molecular phylogenetic analyses with the expanded sampling of Ixeridium and Ixeris including both type species supported the monophyly of each of the genera and the placement of the new species in Ixeridium. The result of the phylogenetic analyses and detailed observation of the chromosome morphology revealed that X = 8 in Ixeridium calcicola is derived from centric fission in an ancestral karyomorphotype with X = 7 in Ixeridium. Ixeridium calcicola and Ixeridium transnokoense formed a Taiwan endemic lineage and their estimated divergence time was in the middle Pleistocene. Their common ancestral lineage may have experienced altitudinal distribution shifts in response to glacial-interglacial temperature fluctuation, and a lineage which had not retreated to alpine ranges in an interglacial period likely survived in a limestone refugium, where ordinary plant species did not grow, leading to allopatric speciation. - Nuclear and plastid DNA data confirm that Sedum tosaense (Crassulaceae) has a disjunct distribution between Pacific mainland Japan and Jeju Island, Korea
Takuro Ito, Koh Nakamura, Chan-Ho Park, Gwan-Pil Song, Ayako Maeda, Yuki Tanabe, Goro Kokubugata
PHYTOTAXA, 177, 4, 221, 230, MAGNOLIA PRESS, 2014年09月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Our molecular phylogeographic analyses based on the nrDNA ITS and cpDNA trnLF of Sedum tosaense populations in the Shikoku District, Japan and Jeju Island, Korea suggested a disjunct distribution. Plants of S. tosaense from the two regions comprised a well-supported clade consisted of plants from Kochi (Shikoku District) and those from Jeju Island; we estimated a divergence time of 0.61 Ma between the Kochi and Jeju populations based on the ITS and partial trnLF. We conclude that: 1) S. tosaense has a disjunct distribution between Kochi and Jeju Island, and 2) plants of this species might have dispersed between Kochi and Jeju Island over water, but not via a land bridge, which flooded before subclade divergence. - Development of EST-derived microsatellite markers in Ophiorrhiza pumila (Rubiaceae) and their application in congeners
Koh Nakamura, Meng-Jung Ho, Chia-Jou Ma, Hsun-An Yang, Ching-I Peng
CONSERVATION GENETICS RESOURCES, 6, 3, 649, 651, SPRINGER, 2014年09月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, In East and Southeast Asian islands, forest area is decreasing and population size of multiple Ophiorrhiza species (Rubiaceae) is diminishing. Here we report the first expressed sequence tag-derived microsatellite markers, which can be applied to wide range of species of the genus. We developed 38 tri- and tetra-nucleotide microsatellite markers based on nonredundant unigenes of O. pumila and tested polymorphism of nine markers using the phylogenetically distant congener O. japonica (n = 96). The number of alleles ranged from 4 to 9. Observed and expected heterozygosities ranged 0.044-0.631 and 0.096-0.674, respectively. The polymorphic information content ranged 0.094-0.626. No null allele, significant deviation from Hardy-Weinberg equilibrium, or significant linkage disequilibrium was observed. Additional tests of cross-species amplification with three other congeners were successful in the nine markers. These markers will be used to investigate genetic diversity and differentiation of island populations of multiple Ophiorrhiza species to build conservation plans. - Begonia jinyunensis (Begoniaceae, section Platycentrum), a new palmately compound leaved species from Chongqing, China
Bo Ding, Koh Nakamura, Yoshiko Kono, Meng-Jung Ho, Ching-I Peng
BOTANICAL STUDIES, 55, e62, SPRINGER, 2014年08月, ［査読有り］, ［責任著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Background: Continental China is the center of Begonia species diversity in Asia and contains more than 60 species out of about 110 named species of section Platycentrum. Mt. Jinyun, located in Chongqing City at the upper reaches of the Yangtze River, harbors a subtropical broadleaved forest with high species diversity. During a botanical survey in Mt. Jinyun, an unknown Begonia species of sect. Platycentrum with palmately compound leaves was collected and studied based on detailed morphological observations and cytological and molecular phylogenetic analyses.
Results: The unknown Begonia bears a superficial resemblance to B. hemsleyana in having palmately compound leaves, a feature unseen in other species of sect. Platycentrum in China. It is however sharply distinct from the latter in the acaulous habit with aerial stems seen only at anthesis and long rhizomes (vs. erect stems to 70 cm or taller with short rhizomes), 4-6 pinnatilobed leaflets with indistinct, decurrent petiolules (vs. 7-10 serrate leaflets with distinct petiolules), and white (vs. pink) tepals. Molecular phylogenetic analyses based on nuclear ribosomal DNA and chloroplast DNA sequences indicated that this species was allied to Platycentrum species occurring in Southwest and South-central China and Vietnam, including B. hemsleyana, and clearly separable from these species. Somatic chromosome number of 2n = 22 was reported for this unknown species. The diploid chromosome number is agreeable with those published for Begonia sect. Platycentrum.
Conclusions: The unknown Begonia is confirmed to be a new species of sect. Platycentrum and hereto described as Begonia jinyunensis C.-I Peng, B. Ding & Q. Wang. - Developing DNA barcodes for species identification in Podophylloideae (Berberidaceae)
Yun-Rui Mao, Yong-Hua Zhang, Koh Nakamura, Bi-Cai Guan, Ying-Xiong Qiu
JOURNAL OF SYSTEMATICS AND EVOLUTION, 52, 4, 487, 499, WILEY-BLACKWELL, 2014年07月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Species of Podophyllum, Dysosma, Sinopodophyllum, and Diphylleia, genera from Podophylloideae of Berberidaceae, have long been used in traditional herbal medicine in East Asia and/or North America. Accurate identification of the species of these four genera is crucial to their medicinal uses. In this study, we tested the utility of nine barcodes (matK, rbcL, atpH-atpI, rpl32-trnL(UAG), rps18-clpp, trnL-trnF, trnL-ndhJ, trnS-trnfM, and internal transcribed spacer (ITS)) to discriminate different species of Podophylloideae. Thirty-six individuals representing 12 species of Podophylloideae were collected from different locations in China, Japan, and North America. We assessed the feasibility of amplification and sequencing of all markers, examined the levels of the barcoding gap based onDNA sequence divergence between ranges of intra-and interspecific variation using pairwise distances, and further evaluated successful identifications using each barcode by similarity-based and tree-based methods. Results showed that nine barcodes, except rps18-clpp, have a high level of primer universality and sequencing success. As a single barcode, ITS has the most variable sites, greater intra-and interspecific divergences, and the highest species discrimination rate (83%), followed by matK which has moderate variation and also high species discrimination rates. However, these species can also be discriminated by ITS alone, except Dysosma versipellis (Hance) M. Cheng ex T. S. Ying and D. pleiantha (Hance) Woodson. The combination of ITS+matK did not improve species resolution over ITS alone. Thus, we propose that ITS may be used as a sole region for identification of most species in Podophylloideae. The failure of ITS to distinguish D. versipellis and D. pleiantha is likely attributed to incomplete lineage sorting due to recent divergence of the two species. - Development and characterization of microsatellite markers in the coffee-family herb Ophiorrhiza japonica (Rubiaceae)
Koh Nakamura, Chia-Jou Ma, Chiun-Jr Huang, Meng-Jung Ho, Hsun-An Yang, Ching-I Peng
CONSERVATION GENETICS RESOURCES, 6, 2, 447, 449, SPRINGER, 2014年06月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Ophiorrhiza japonica (Rubiaceae) in Taiwan shows altitudinal morphological differentiation, suggesting altitudinal genetic differentiation. However, global warming and/or road building are possibly causing human-mediated altitudinal gene flow and genetic disturbance. To investigate this possibility, we developed the first nuclear microsatellite markers for the genus. Using the low-cost compound microsatellite marker technique, 17 markers were developed and nine markers were polymorphic. The number of alleles per polymorphic locus ranged from 2 to 13. At population level, observed (H (o) ) and expected (H (e) ) heterozygosities ranged 0.070-0.831 and 0.068-0.812, respectively. The polymorphic information content ranged 0.066-0.787. No null allele, significant deviation from Hardy-Weinberg equilibrium, or significant linkage disequilibrium was observed. The 17 marker loci were successfully PCR-amplified in three congeneric species except for three cases. The developed microsatellite markers will be used to investigate genetic structure and gene flow among O. japonica populations at different altitudes. - In situ glacial survival at the northern limit of tropical insular Asia by a lowland herb Begonia fenicis (Begoniaceae)
Koh Nakamura, Goro Kokubugata, Rosario Rivera Rubite, Chiun-Jr Huang, Yoshiko Kono, Hsun-An Yang, Auriea Lopez-Feliciano, Mina L. Labuguen, Masatsugu Yokota, Ching-I Peng
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY, 174, 3, 305, 325, WILEY-BLACKWELL, 2014年03月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, Equatorward contraction and re-expansion during and after the last glacial maximum has been reported for temperate plants, but little is known about latitudinal shifts of tropical plants in insular Asia. We used molecular methods to test the post-glacial migration hypothesis for Begonia fenicis at the northern limit of tropical insular Asia, namely, the northern Philippines, southern Taiwan and the southern Ryukyus of Japan. Based on internal transcribed spacer sequences, analyses of phylogeographical structure suggested long-term survival of island populations and negated recent northward migration; demographic analyses indicated spatial expansion in the early Pleistocene and subsequent stable demography. Based on nuclear microsatellites, Bayesian clustering and other spatial analyses indicated inter-island differentiation, and coalescent analysis of migration indicated the lack of inter-island migration during and after the last glacial maximum. Our results refuted the postglacial migration hypothesis for B. fenicis and suggest in situ survival through the last glacial maximum. The stable historical distribution and demography are probably attributable to the Kuroshio Current bringing warm and humid air, which washed these islands even during the glacial periods. Additionally, because these are low islands, absence of competition with montane plants descending to the lowlands during cooler periods may have mitigated the influence of temperature lowering and facilitated their survival. (c) 2014 The Linnean Society of London, Botanical Journal of the Linnean Society, 2014, 174, 305-325. - Molecular phylogenetic distinction between Taiwan endemic Rhynchotechum brevipedunculatum and R. discolor (Gesneriaceae) widespread in subtropical and tropical Asia
Goro Kokubugata, Koh Nakamura, Yukiko Saito, Mina L. Labuguen, Auriea Lopez-Feliciano, Masatsugu Yokota
Bulletin of the National Museum of Nature and Science. Series B, Botany, 40, 1, 47, 53, National Museum of Nature and Science, 2014年, ［査読有り］, ［国際共著］, ［国内誌］
英語 - Molecular and morphological data reconfirm the occurrence of two varieties of Helwingia japonica subsp. japonica (Helwingiaceae) in Kochi Prefecture, Japan
Hana Umemoto, Koh Nakamura, Akiko Maeda, Masatsugu Yokota, Goro Kokubugata
Bulletin of the National Museum of Nature and Science. Series B, Botany, 40, 4, 153, 161, National Museum of Nature and Science, 2014年, ［査読有り］, ［国内誌］
英語 - First record of the genus Limonium (Plumbaginaceae) from the Malesian region
Koh Nakamura, Rosario Rivera Rubite, Goro Kokubugata, Yoshiko Kono, Masatsugu Yokota, Ching-I Peng
PHYTOTAXA, 152, 1, 56, 58, MAGNOLIA PRESS, 2013年12月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語 - Begonia tandangii (Begoniaceae, section Baryandra), a new species from Luzon Island, the Philippines
Koh Nakamura, Rosario Rivera Rubite, Yoshiko Kono, John Rey Callado, Ching-I Peng
PHYTOTAXA, 145, 1, 27, 37, MAGNOLIA PRESS, 2013年11月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, We describe Begonia tandangii, a new species of Begonia sect. Baryandra from the Sierra Madre Mountain Range of Luzon Island, the Philippines. Begonia tandangii has a close resemblance to B. fenicis in gross morphology, differing in having leaf margin sparsely fringed with minute hairs (vs. glabrous or with minute hairs only on teeth) and capsules with broadly-ovate outline and an acuminate apex (vs. capsules with broadly-obovate outline and a rounded to truncate apex). Phylogenetic analyses of Philippines species of sect. Baryandra based on ITS sequences revealed that B. tandangii was clearly separated from B. fenicis. Begonia tandangii is currently known only from the type locality in a coastal forest of Baler, Aurora Province, which is in the neighborhood of Aurora Memorial National Park. - Taxonomic reexamination of Portulaca okinawensis (Portulacaceae) in the Ryukyu Archipelago of Japan based on molecular and morphological data
Goro Kokubugata, Koh Nakamura, Yumiko Hirayama, Masatsugu Yokota
PHYTOTAXA, 117, 1, 11, 22, MAGNOLIA PRESS, 2013年07月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, We used molecular phylogenetic and morphological data to reexamine the taxonomic status of Portulaca okinawensis (equivalent to P. pilosa subsp. okinawensis) endemic to the central Ryukyu islands, southwestern Japan. Our molecular analyses showed that P. okinawensis is monophyletic, sister to the clade of P. psammotropha and P. tuberosa and it is not closely related to P. pilosa subsp. pilosa. Two subclades, one comprising plants from the Okinawa Islands and the other from the Amami Islands, were recognized. The plants from the Okinawa Islands had more than 20 stamens, orange-yellow nallowly obovate to oblanceolate petals, and reddish-green stems (as the holotype), while the plants from the Amami Islands had less than 20 stamens, lemon-colored obovate petals, and bright-green stems. The molecular and morphological data support a taxonomic treatment of Walker & Tawada (1951) regarding P. okinawensis as a separate species, also suggesting that the plants from the Amami and Okinawa islands should be treated as different taxa. A new variety Portulaca okinawensis var. amamiensis was here described. - Correction of Confusions Regarding the Identity and Synonymy of Youngia (Asteraceae: Tribe Cichorieae) in Taiwan
Koh Nakamura, Yoshiko Kono, Chiun-Jr Huang, Kuo-Fang Chung, Ching-I Peng
SYSTEMATIC BOTANY, 38, 2, 507, 516, AMER SOC PLANT TAXONOMISTS, 2013年04月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Youngia japonica, the type species for its genus, has the widest distribution in the genus and is invasive worldwide. Recent molecular phylogenetic analysis supported recognition of three ecologically differentiated entities: Y. japonica subsp. formosana, Y. japonica subsp. japonica, and an undescribed morphological variant thought to be a new subspecies in Taiwan. These are the only infraspecific taxa currently recognized in this morphologically variable species. Use of proper names for these taxa and related species is important for future revision of Y. japonica in other regions, for nomenclature and biogeography of the genus, and for activities to control its invasiveness. Careful examination of the holotype of Y. japonica subsp. formosana and relevant literature revealed that the name has long been misapplied to Youngia in mountain ranges in Taiwan; it should be applied to the morphological variant growing on littoral, raised coral reefs in southwestern Taiwan. The misidentified entity is here described as Youngia japonica subsp. monticola. In addition, Y. taiwaniana was treated as a synonym of Y. longiflora (equivalent to Y. japonica subsp. longiflora) in the Flora of Taiwan, but the holotype has achenes with a long beak and does not belong to Youngia. It is here treated as a synonym of Ixeridium laevigatum. A key to Youngia in Taiwan is provided. - Migration of Lobelia zeylanica (Campanulaceae) from Taiwan to the Ryukyu Archipelago inferred from chloroplast DNA data
Goro Kokubugata, Yukiko Saito, Koh Nakamura, Auriea Lopez-Feliciano, Mina L. Labuguen, Masatsugu Yokota
Bulletin of the National Museum of Nature and Science. Series B, Botany, 39, 2, 95, 100, National Museum of Nature and Science, 2013年, ［査読有り］, ［国際共著］, ［国内誌］
英語 - Molecular phylogeography reveals an antitropical distribution and local diversification of Solenogyne (Asteraceae) in the Ryukyu Archipelago of Japan and Australia.
Nakamura K, Denda T, Kokubugata G, Forster PI, Wilson G, Peng CI, Yokota M
Biological Journal of the Linnean Society, 105, 1, 197, 217, WILEY-BLACKWELL, 2012年12月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, An antitropical distribution represents an intriguing disjunction, in which a given species or sister lineages occupy regions north and south of the tropics but are absent from the intervening areas. Solenogyne mikadoi endemic to the Ryukyu Archipelago is regarded as an Australian element. Testing the phylogenetic relationship with Australian congeners and discussing the onset timing and causes of the disjunction would potentially enhance the understanding of antitropical distribution. A nuclear ribosomal DNA phylogeny was reconstructed using Bayesian and most parsimonious criteria with allied genera. Solenogyne was monophyletic and clustered with Lagenophora huegelii endemic to Australia, indicating the antitropical distribution and Australian origin of Solenogyne. Multispecies coalescent analysis based on nuclear ribosomal DNA and chloroplast DNA indicated the divergence of S. mikadoi and Australian congeners in the Plio-Pleistocene. Phylogenetic network analyses suggested that the ancestral lineage of S. mikadoi first colonized the southernmost island in the archipelago and then dispersed northward. The migration to the archipelago likely followed the flourishing of Solenogyne in open vegetation communities that radiated in south-eastern Australia during the late Pliocene. This disjunction might arise through long-distance dispersal across the tropics or, alternatively, through extinction in the tropics as a result of unsuitably high temperatures during climate oscillation and/or competitions from diverse tropical flora surviving since the early Tertiary. (C) 2011 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 105, 197217. - Cassytha pubescens and C. glabella (Lauraceae) are not disjunctly distributed between Australia and the Ryukyu Archipelago of Japan - evidence from morphological and molecular data
Goro Kokubugata, Koh Nakamura, Paul I. Forster, Gary W. Wilson, Ailsa E. Holland, Yumiko Hirayama, Masatsugu Yokota
AUSTRALIAN SYSTEMATIC BOTANY, 25, 5, 364, 373, CSIRO PUBLISHING, 2012年10月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Morphological comparisons and molecular phylogenetic analyses were conducted to resolve taxonomic confusion in Cassytha glabella and C. pubescens, both of which were first described from Australia and subsequently considered to be disjunctly distributed between Australia and the Ryukyu Archipelago of Japan. In the morphological comparisons, plants considered as C. pubescens in the Ryukyus differ from C. pubescens in Australia in the presence or absence of hairs on the petals, and those considered as C. glabella in the Ryukyus differ from the C. glabella in Australia in bract and peduncle morphology. The molecular analyses indicated that plants attributed to C. pubescens in the Ryukyus were not closely related to C. pubescens in Australia, and were nested in a clade of populations of a Pan-Western Pacific species C. filiformis. Plants attributed to C. glabella in the Ryukyus were distantly related to C. glabella in Australia. We concluded that plants considered as C. pubescens and C. glabella in the Ryukyus are to be respectively treated as C. filiformis and the Ryukyu endemic species C. pergracilis. - Late Pleistocene lineage divergence among populations of Neolitsea sericea (Lauraceae) across a deep sea-barrier in the Ryukyu Islands
Sheng-Nan Zhai, Hans Peter Comes, Koh Nakamura, Hai-Fei Yan, Ying-Xiong Qiu
JOURNAL OF BIOGEOGRAPHY, 39, 7, 1347, 1360, WILEY-BLACKWELL, 2012年07月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Aim Our goals were: (1) to assess the population genetic structure and demographic divergence history of a bird-dispersed tree, Neolitsea sericea, endemic to East Asian land-bridge islands; and (2) to interpret the results in the light of controversies over the dating and configurations of land bridges through the Japanese Ryukyu Island Arc. Location JapanRyukyuTaiwan Island Arc and Chinese/Korean offshore islands. Methods We applied 10 nuclear microsatellites (nSSRs) and one chloroplast (cp) DNA sequence marker (psbAtrnH intergenic spacer) to 31 populations (397 and 326 individuals, respectively) from throughout the species range to infer current patterns of genetic diversity and structure, and pollen-to-seed migration ratios (r). A coalescent-based isolation-with-migration (IM) model was applied to the combined nSSR/cpDNA data set to estimate lineage divergence times and population demographic parameters. Results The geographic structure of nSSRs and the distribution of most cpDNA haplotypes revealed two distinct lineages located in areas north and south of the Tokara Gap, a narrow (c. 37 km wide) but deep (> 1000 m) sea-strait between the northern and central Ryukyus. Based on the IM analyses, we (1) dated the divergence of these northern and southern lineages to c. 0.07 Ma (90% highest posterior density interval: 0.020.38 Ma); (2) estimated a slightly smaller effective population size for the northern compared to the southern lineage; and (3) recovered only trivial signals of post-divergence gene flow between them. Main conclusions The estimated divergence time for northern and southern lineages is consistent with geological evidence for the existence of land connections in the Tokara region during cold stages of the latest Pleistocene; it is thus incompatible with an ancient sea-barrier hypothesis for the Ryukyu Arc, where we would have expected much older divergences related to the initial formation of the Tokara and Kerama tectonic straits during the Pliocene. Multiple factors are likely to have had a role in the divergence of N. sericea, including not only land-bridge submergence, but also island configuration, and/or constraints on adaptation along a latitudinal temperature gradient. - Extreme habitats that emerged in the Pleistocene triggered divergence of weedy Youngia (Asteraceae) in Taiwan
Koh Nakamura, Kuo-Fang Chung, Chiun-Jr Huang, Yoshiko Kono, Goro Kokubugata, Ching-I Peng
MOLECULAR PHYLOGENETICS AND EVOLUTION, 63, 2, 486, 499, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012年05月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Weeds with broad distributions and large morphological variation are challenging for systematists and evolutionarily intriguing because their intensive dispersal would likely prevent local morphological differentiation. Study on weeds will help to understand divergence in plants unlikely to be affected by geographical and ecological barriers. We studied Youngia japonica based on nrDNA and cpDNA sequences. This is a widespread native in Asia and invasive worldwide: nevertheless, three subspecies (japonica, longiflora, and formosana) and an undescribed variant occur in Taiwan.
Bayesian and the most parsimonious phylogenies revealed that subspecies longiflora is a different linage and independently arrived in Taiwan during the Pleistocene via land connection to the Asian Continent. Bayesian time estimation suggested that Youngia in Taiwan diverged in the lower Pleistocene or more recently. Extreme habitats that emerged in the Pleistocene, i.e., cold mountain ranges for subspecies formosana and xeric, raised coral reefs for the undescribed Youngia variant probably had triggered the divergence.
Components of Youngia in Taiwan are not monophyletic; a coalescent-based test suggested incomplete lineage sorting. Nevertheless, the samples within each taxon share unique morphological features suggesting a common gene pool and each taxon has different dominant ITS and/or cpDNA types; these conditions suggest ongoing process toward monophyly via coalescent processes and support the delimitation of intraspecific taxa. (C) 2012 Elsevier Inc. All rights reserved. - DEVELOPMENT OF MICROSATELLITE MARKERS IN HETEROSTYLOUS HEDYOTIS CHRYSOTRICHA (RUBIACEAE)
Na Yuan, Yi Sun, Koh Nakamura, Ying-Xiong Qiu
AMERICAN JOURNAL OF BOTANY, 99, 2, E43, E45, BOTANICAL SOC AMER INC, 2012年02月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Premise of the study: Microsatellite primers were developed for a heterostylous herb, Hedyotis chrysotricha to investigate the effect of habitat fragmentation on its genetic diversity and population structure.
Methods and Results: Twelve primer sets were developed and their polymorphisms were tested on 47 individuals from two island populations of H. chrysotricha in Thousand Island Lake of China. The number of alleles per locus ranged from five to 10, with an average of seven alleles. Expected heterozygosity per locus ranged from 0.284 to 0.821 and observed heterozygosity ranged from 0.191 to 0.851.
Conclusions: We showed that all of the 12 microsatellite markers developed for H. chrysotricha are polymorphic within populations, which should provide a powerful tool for assessing population structure and genetic diversity across fragmented and continuous populations, and for studying the genetic effects of habitat fragmentation on this species. - Antitropical distribution of Lobelia species (Campanulaceae) between the Ryukyu Archipelago of Japan and Oceania as indicated by molecular data
Goro Kokubugata, Koh Nakamura, Paul I. Forster, Yumiko Hirayama, Masatsugu Yokota
AUSTRALIAN JOURNAL OF BOTANY, 60, 5, 417, 428, CSIRO PUBLISHING, 2012年, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, We tested the antitropical distribution of Lobelia loochooensis, endemic to the Ryukyu Archipelago of Japan, and its putative sister species of the same section Hypsela in Oceania (Australia and New Zealand). Molecular phylogenetic analyses based on cpDNA sequences were conducted for 41 Lobelia species of 11 sections including all the species of sect. Hypsela investigated in Asia and Malesia and 32 species of 16 allied genera of family Campanulaceae, because the genus Lobelia is known to be polyphyletic. In the result, L. loochooensis and an Australian endemic L. fluviatilis formed a clade, and this clade was sister to a clade of four New Zealand endemics: L. carens, L. fatiscens, L. fugax and L. ionantha. These two clades were nested in a clade with two other Australian congeners. We conclude that: (1) the lineage of L. loochooensis and the five Lobelia species occurring in Oceania demonstrate an antitropical distribution pattern; and (2) L. loochooensis has likely originated from a dispersal event from Australia to the Ryukyu Archipelago. - 琉球及び台湾の植物地理（第11回日本植物分類学会奨励賞受賞記念論文）
中村剛
分類−日本植物分類学会誌, 12, 2, 117, 139, 日本植物分類学会, 2012年, ［査読有り］, ［招待有り］, ［筆頭著者］, ［国内誌］
日本語 - ISOLATION OF COMPOUND MICROSATELLITE MARKERS IN BEGONIA FENICIS (BEGONIACEAE) ENDEMIC TO EAST AND SOUTHEAST ASIAN ISLANDS
Koh Nakamura, Chiun-Jr Huang, Rosario Rivera Rubite, Wai-Chao Leong, Yoshiko Kono, Hsun-An Yang, Ching-I Peng
AMERICAN JOURNAL OF BOTANY, 99, 1, E20, E23, BOTANICAL SOC AMER INC, 2012年01月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Premise of the study: We developed compound microsatellite markers for Begonia fenicis, a species endemic to eastern and southeastern Asian islands, to investigate geographical genetic structure.
Methods and Results: Using the compound microsatellite marker technique, 21 markers were developed and six polymorphic markers were characterized for samples from four islands in Taiwan and southern Japan. The number of alleles per locus ranged from two to six (mean = 4.33). Observed and expected heterozygosities were 0.125-0.725 (0.383) and 0.498-0.719 (0.641), and polymorphic information content was 0.371-0.664 (0.567). The interspecific transferability of the 21 markers was evaluated for eight species of the section Diploclinium from the Philippines; 15 markers were successfully amplified in one to eight species.
Conclusions: These results indicate the utility of the six microsatellite markers in B. fenicis to investigate geographical genetic structure. The transferable markers are potentially useful for other species of the section. - The architectural stratification and woody species diversity in subtropical evergreen broadleaf forests along a latitudinal thermal gradient of the Ryukyu Archipelago, Japan
S. M. Feroz, Rempei Suwa, Koh Nakamura, Akio Hagihara, Masatsugu. Yokota
Raffles Bulletin of Zoology, In press, 2011年, ［査読有り］, ［国際共著］, ［国際誌］ - Taxonomic reconsideration and phytogeographic implications for Nertera yamashitae (Rubiaceae), endemic to Amami Island of the Ryukyu Archipelago, Japan
Goro Kokubugata, Koh Nakamura, Yumiko Hirayama, Ching-I Peng, Masatsugu Yokota
Bulletin of the National Museum of Nature and Science. Series B, Botany, 37, 1, 9, 22, National Museum of Nature and Science, 2011年, ［査読有り］, ［国際共著］, ［国内誌］
英語 - The impacts of historical barriers on floristic patterns of plant groups with different dispersal abilities in the Ryukyu Archipelago, Japan
Koh Nakamura, Rempei Suwa, Tetsuo Denda, Masatsugu Yokota
Raffles Bulletin of Zoology, In press, 2011年, ［査読有り］, ［筆頭著者］, ［国際誌］ - ISOLATION OF COMPOUND MICROSATELLITE MARKERS FOR THE ENDANGERED PLANT NEOLITSEA SERICEA (LAURACEAE)
Sheng-Nan Zhai, Xiao-Ling Yan, Koh Nakamura, Misako Mishima, Ying-Xiong Qiu
AMERICAN JOURNAL OF BOTANY, 97, 12, E139, E141, BOTANICAL SOC AMER INC, 2010年12月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Premise of the study: The development of compound microsatellite markers was conducted in Neolitsea sericea to investigate genetic diversity and population genetic structure of this endangered insular species.
Methods and Results: Using the compound microsatellite marker technique, 10 compound microsatellite markers that were successfully amplified showed polymorphism when assessed in 55 individuals from two populations in East China and Japan. Overall, the number of alleles ranged from 3 to 17, with an average of 7.9 alleles per locus. In addition, these primers could be easily amplified in Neolitsea aurata var. paraciculata and N. aurata var. chekiangensis.
Conclusions: The highly polymorphic markers developed and characterized in this study will be useful for population genetic studies of N. sericea. - Phylogeography of Ophiorrhiza japonica (Rubiaceae) in continental islands, the Ryukyu Archipelago, Japan
Koh Nakamura, Tetsuo Denda, Goro Kokubugata, Rempei Suwa, T. Y. Aleck Yang, Ching-I Peng, Masatsugu Yokota
JOURNAL OF BIOGEOGRAPHY, 37, 10, 1907, 1918, WILEY-BLACKWELL PUBLISHING, INC, 2010年10月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Aim Phylogeographical patterns in the Ryukyu Archipelago have been explained primarily by landbridge formation and the opening of two straits in the Pliocene, namely the Tokara and Kerama gaps. These old straits have been considered to be the barriers most likely to determine genetic boundaries. To test this, we conducted a molecular analysis of the herb Ophiorrhiza japonica. We discuss the causes of and processes involved in its phylogeographical structure and explore aspects of island separation other than the duration of the straits to explain genetic boundaries at the gaps.
Location Ryukyu Archipelago, Japan.
Methods Plants were collected from 40 localities in the archipelago and vicinity. Non-coding regions of chloroplast DNA were sequenced. The genealogical relationships among haplotypes were estimated using a statistical parsimony network. To examine the phylogeographical structure, we compared two parameters of population differentiation, namely G(ST) and N(ST), and conducted correlation analysis of genetic and geographical distances. Genetic boundaries were identified using Monmonier's maximum difference algorithm. To test vicariance-dispersal hypotheses, that is, vicariance after migration via the Pliocene landbridge or over-sea dispersal in the Pleistocene, molecular dating analysis was conducted.
Results A statistical parsimony network revealed that the haplotypes from the Ryukyu Archipelago and northwards coalesce to one ancestral haplotype in Taiwan. A clear phylogeographical structure was observed: plants within the same population and populations in geographical proximity were phylogenetically close. A genetic boundary was recognized across the Kerama Gap, but not across the Tokara Gap. Dating analysis suggested that population divergence across the Kerama Gap occurred in the early to late Pleistocene.
Main conclusions The statistical parsimony network suggests migration from Taiwan and northward range expansion in the archipelago. Based on the divergence time, over-sea dispersal in the Pleistocene is likely, although migration via a Pliocene landbridge is not totally rejected. Negligible genetic differentiation across the Tokara Gap suggests recent over-sea dispersal, possibly facilitated by the small geographical width of the gap. Conversely, the large genetic differentiation across the Kerama Gap is probably explained by the large geographical distance across it. The past splitting of a landbridge would have had a significant influence on population differentiation after a certain geographical distance was reached. - Evidence of three parallel evolutions of leaf dwarfism and phytogeography in Lysimachia sect. Nummularia in Japan and Taiwan
Goro Kokubugata, Koh Nakamura, Wataru Shinohara, Yukiko Saito, Ching-I Peng, Masatsugu Yokota
MOLECULAR PHYLOGENETICS AND EVOLUTION, 54, 2, 657, 663, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2010年02月, ［査読有り］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Phylogenetic analyses were performed using nrITS variations of eight species including one variety of the genus Lysimachia sect. Nummularla in Japan and Taiwan, with another 34 Lysimachia species and Glaux maritime obtained from a DNA database. Among the four dwarf-leaf taxa in Japan and Taiwan, L. japonica var. minutissima of Japan and two Taiwanese species were placed into two different clades: and L. liukiuensis of Japan, was positioned outside of these two clades. These results suggest that three independent dwarfism events generated the four dwarf-leaf taxa; and that there were likely multiple migration events from China to Japan and Taiwan. (C) 2009 Elsevier Inc. All rights reserved. - Geohistorical and current environmental influences on floristic differentiation in the Ryukyu Archipelago, Japan
Koh Nakamura, Rempei Suwa, Tetsuo Denda, Masatsugu Yokota
JOURNAL OF BIOGEOGRAPHY, 36, 5, 919, 928, WILEY-BLACKWELL, 2009年05月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Floristic differentiation in the Ryukyu Archipelago has been explained primarily by geohistory, specifically landbridge formation and vicariance at the Tokara and Kerama Gaps, two deep-sea channels through the island arc. This ignores current environmental effects, which may also be important. We therefore tested whether the floristic differentiation pattern is explained primarily by the historical effect of the gaps as barriers, or whether a better understanding of floristic differentiation is achieved when both historical and current environmental factors are incorporated.
Ryukyu Archipelago, Japan: an assemblage of continental islands.
We compiled a presence-absence matrix of 1815 plant species on 26 islands. Floristic dissimilarity distances between islands were calculated using Simpson's similarity index and analysed using cluster analysis. We also conducted multiple regression on distance matrices (MRM) to examine the significance of the historical factors of the gaps and current environmental factors: geographical distance among islands and differences in island area and maximum elevation.
We detected clear patterns of floristic differentiation across the gaps. Using the two gaps as explanatory variables, the MRM showed that both had significant effects on floristic dissimilarity distance. However, when geographical distance was added to the explanatory model, the Kerama Gap effect disappeared. When all five explanatory variables were used, the Tokara Gap and geographical distance had positive effects, but area difference had a negative effect. The Kerama Gap and difference in maximum elevation had no effect.
The geographical pattern of floristic differentiation appears to indicate the influence of both gaps. However, the MRM indicates that the floristic differentiation across the Kerama Gap is no more than could be explained solely by geographical distance. Across the Tokara Gap, however, floristic differentiation is larger than geographical distance alone can explain. This additional differentiation is attributable to the effect of the historical barrier. To verify the significance of historical effects of vicariance on island biota, the confounding effects of geographical distance must be considered. The distance decay of floristic similarity and negative effect of area difference on floristic differentiation demonstrate that floristic differentiation is better understood by incorporating both historical and current environmental factors. - Floristic composition, woody species diversity and spatial distribution of trees on the basis of architectural stratification in a subtropical evergreen broadleaf forest on Ishigaki Island of the Ryukyu Archipelago, Japan
S. M. Feroz, Min Wu, Sahadev Sharma, Yan Li, Rempei Suwa, Koh Nakamura, Akio Hagihara, Tetsuo Denda, Masatsugu Yokota
Tropics, 18, 103, 114, 2009年, ［査読有り］, ［国際共著］, ［国内誌］ - 「世界の侵略的外来種ワースト100」の1種センニンサボテンOpuntia stricta（サボテン科）の北大東島への侵入とその防除法
中村剛, 佐藤亜希子, 小林峻, 伊澤雅子, 傳田哲郎, 横田昌嗣
分類−日本植物分類学会誌, 9, 2, 159, 165, 日本植物分類学会, 2009年, ［査読有り］
日本語 - 花冠裂片数の変異に基づくヘツカリンドウ（リンドウ科）の２変種
中村剛, 國府方吾郎, 傳田哲郎, 横田昌嗣
植物地理・分類研究, 56, 1, 33, 34, 2008年 - Breakdown of distyly in a tetraploid variety of Ophiorrhiza japonica (Rubiaceae) and its phylogenetic analysis
Koh Nakamura, Tetsuo Denda, Osamu Kameshima, Masatsugu Yokota
JOURNAL OF PLANT RESEARCH, 120, 4, 501, 509, SPRINGER TOKYO, 2007年07月, ［査読有り］, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, We examined the floral morph of tetraploid Ophiorrhiza japonica Blume var. amamiana Hatus. and diploid O. japonica var. japonica to elucidate the association of distyly and ploidy levels. Chloroplast DNA phylogeny was reconstructed to determine the number of tetraploidization events and floral morph shifts in O. japonica. All individuals of O. japonica var. amamiana proved to be long-homostylous, whereas O. japonica var. japonica was distylous with typical long- and short-styled flowers. Distyly is related to the ploidy level. The bagging treatment of flowers indicated that O. japonica var. amamiana is self-compatible and potentially automatically self-pollinating. In cpDNA sequencing analysis, no haplotype was shared between the two varieties. The cpDNA haplotype network displayed the monophyly of O. japonica var. amamiana, suggesting a single origin of this variety. Hence, both tetraploidization and the breakdown of distyly to homostyly in O. japonica var. amamiana likely occurred just once. Because O. japonica var. amamiana having the morphological and cytological entity is recognized as a single lineage and clearly separated from O. japonica var. japonica, this variety can be considered to be a distinct species. We therefore propose to raise O. japonica var. amamiana to the rank of species. - Phylogenetic systematics of the monotypic genus Hayataella (Rubiaceae) endemic to Taiwan
Koh Nakamura, Shih-Wen Chung, Goro Kokubugata, Tetsuo Denda, Masatsugu Yokota
JOURNAL OF PLANT RESEARCH, 119, 6, 657, 661, SPRINGER TOKYO, 2006年11月, ［査読有り］, ［筆頭著者］, ［国際共著］, ［国際誌］
英語, 研究論文（学術雑誌）, Hayataella (Rubiaceae) is a monotypic genus endemic to Taiwan that comprises H. michelloides. In recent years, Hayataella was considered to be synonymous with Ophiorrhiza; however, no specific data have been reported, and the systematic treatment of Hayataella has been unclear. To elucidate the systematic treatment of Hayataella, molecular phylogenetic analyses based on ITS of nrDNA, atp B-rbcL, and trnK/matK of cpDNA were conducted with four Ophiorrhiza species. In the Bayesian and the most parsimonious trees, H. michelloides was included in the Ophiorrhiza clade. The monotypic status of Hayataella is, therefore, not considered appropriate, and the combination Ophiorrhiza michelloides (Masam.) H. S. Lo is supported. - Karyotype of Ophiopogon reversus (Convallariaceae) from Taiwan and the Southern Ryukyus
Tetsuo Denda, Koh Nakamura, Masatsugu Yokota
Taiwania, 51, 117, 122, 2006年, ［査読有り］, ［国際誌］ - Homostyly and autogamy in Ophiorrhiza pumila (Rubiaceae) in the Ryukyu Archipelago
Koh Nakamura, Tetsuo Denda, Masatsugu Yokota
Journal of Japanese Botany, 81, 2, 113, 120, 津村研究所, 2006年, ［査読有り］, ［筆頭著者］, ［国内誌］
英語 - 琉球列島産ソテツCycas revoluta Thunberg（ソテツ科Cycadaceae）の種子形態
中村剛, 傳田哲郎, 横田昌嗣
沖縄生物学会誌, 43, 53, 57, 2005年, ［査読有り］
書籍等出版物
- 礼文島維管束植物に関するレッドデータブック
佐藤謙, 宮本誠一郎, 村上賢治, 中川博之, 首藤光太郎, 山崎真美, 中村剛
礼文町, 2023年03月, 87, 日本語, 調査報告書, ［共著］ - 通往世界的植物: 臺灣高山植物的時空旅史
游旨价著, 中村剛, 李建成, 李攀, 趙建棣, 鍾國芳審訂, 臺灣，琉球群島與日本列島間的聯繋
春山出版, 2020年03月, ［国際共著］, ［分担執筆］ - 「琉球の植物」データベース
琉球の植物研究グループ(阿部篤志, 海老原 淳, 國府方吾郎, 齊藤由紀子, 中村剛, 横田昌嗣
2018年, 日本語 - 須崎忠助植物画集-大雪山植物其他
加藤克, 高橋英樹, 中村剛, 早川尚
北海道大学出版会, 2016年06月 - 北海道の自然を特徴づける湿地植物の保全遺伝学的研究
中村剛, 田村紗彩
2016年, 日本語
講演・口頭発表等
- Challenges and approaches in living-collection data management for conservation in botanic gardens in Japan
Koh Nakamura
East Asia Biodiversity Conservation Network（EABCN）Academic Workshop 2024, 2024年10月10日, East Asia Biodiversity Conservation Network（EABCN）, 英語, 口頭発表（基調）
Shenyang, China, 中華人民共和国, ［招待講演］, ［国際会議］ - 北海道-東北アジアの植物の保全
中村 剛
国際シンポジウム 人と自然の共生―植物園の役割を考える, 2024年10月06日, 京都府, 日本語, シンポジウム・ワークショップパネル（指名）
京都府，京都市, 日本国, ［招待講演］, ［国際会議］ - 北日本に優占するハルニレは南方系：遺伝構造と低温応答の緯度分化が示す分布拡大仮説
岩佐真彦, 村井良徳, 伊東拓朗, 岩泉正和, Kwak Myounghai, Elena A. Marchuk, Pavel V. Krestov, Svetlana N. Bondarchuk, Nataliya K. Kovtonyuk, Ma Jinshuang, Xing Yaowu, Yu Chih-Chieh, 福澤加里部, 山本福壽, 福田知子, 國府方吾郎, 中村剛
日本植物分類学会第23回大会, 2024年03月10日, 日本語, 口頭発表（一般）
宮城県，仙台市, 日本国, ［国内会議］, ［国際共著］ - 北海道の石灰岩地に固有の国内希少種シリベシナズナの遺伝的管理単位と栽培土壌条件
髙橋尚敏, 佐藤謙, 中川博之, 渡部敏裕, 平田聡之, 國府方吾郎, 村井良徳, 中村剛
日本植物分類学会第23回大会, 2024年03月10日, 日本語, 口頭発表（一般）
宮城県，仙台市, 日本国, ［国内会議］ - 植物園におけるコレクション管理の問題点と、個体管理データベースの希少種保全における活用事例
中村剛
日本植物園協会環境省連携事業データベース研修会, 2024年03月06日, 日本語, 公開講演，セミナー，チュートリアル，講習，講義等
オンライン, 日本国, ［招待講演］, ［国内会議］ - 日本列島・琉球列島の植物地理
中村剛
種生物学会和文誌シンポジウム「古くて新しい島の生物学」, 2023年12月03日, 日本語, シンポジウム・ワークショップパネル（指名）
愛知県，岡崎市, 日本国, ［招待講演］, ［国内会議］ - The ecological divergence processes of Anaphalis margaritacea var. yedoensis (Asteraceae), exclusively inhabiting gravel riverbanks, inferred by demographic modeling analysis (P9)
Taishi Hoson, Takuro Ito, Shinji Fujii, Daiki Takahashi, Koh Nakamura, Yoshihisa Suyama, Masayuki Maki
The 10th East Asian Plant Diversity and Conservation Symposium 2023 (Osaka), 2023年10月28日, 英語, ポスター発表
2023年10月28日 - 2023年10月29日, Osaka city, Osaka Pref., 日本国, ［国際会議］ - Problems for conservation in record management of living collections of Japanese botanic gardens (P17)
Saya (Tamura)Asano, Tomohisa Yukawa, Koh Nakamura
The 10th East Asian Plant Diversity and Conservation Symposium 2023 (Osaka), 2023年10月28日, 英語, ポスター発表
2023年10月28日 - 2023年10月29日, Osaka city, Osaka Pref., 日本国, ［国際会議］ - Impact of gene flow on the evolution of Cycas revoluta Thunb. (P25)
Jui-Tse Chang, Koh Nakamura, Chien-Ti Chao, Min-Xin Luo, Pei-Chun Liao
The 10th East Asian Plant Diversity and Conservation Symposium 2023 (Osaka), 2023年10月28日, 英語, ポスター発表
2023年10月28日 - 2023年10月29日, Osaka city, Osaka Pref., 日本国, ［国際会議］, ［国際共著］ - Botanic garden collections in Japan for conservation: progress and problems of living collections
Koh Nakamura
International Conference on the Protection and Application of Plant Diversity in Northern Hemispheres, 2023年10月25日, 英語, 口頭発表（招待・特別）
Beijing, China, 中華人民共和国, ［招待講演］, ［国際会議］ - 北大植物園における保全と他園との連携：エンビセンノウ（ナデシコ科）の例
中村剛
日本植物園協会シンポジウム「植物園における植物多様性保全」, 2023年10月01日, 日本語, シンポジウム・ワークショップパネル（指名）
大阪府，交野市, 日本国, ［国内会議］ - 日本植物園協会「植物個体管理データベース」への登録と二次元バーコード付き植物ラベルの作成：北海道大学植物園における試行例
板羽貴史, 持田大, 稲川博紀, 大野祥子, 永谷工, 高田純子, 浅野(田村)紗彩, 中村 剛
日本植物園協会第58回大会, 2023年05月30日, 日本語, 口頭発表（一般）
高知県，高知市, 日本国, ［国内会議］ - ヒルムシロ属新雑種と複数の北海道新産種を含む茨戸川の水生植物相
中里清孝, 山崎真実, 中村剛, 首藤光太郎
日本植物分類学会第22回大会, 2023年03月05日, 日本語, 口頭発表（一般）
千葉県千葉市, 日本国, ［国内会議］ - 礫河原環境に特異的に生育するカワラハハコの起源を探る
保尊大志, 伊東拓朗, 中村剛, 藤井伸二, 高橋大樹, 陶山佳久, 牧雅之
日本植物分類学会第22回大会, 2023年03月03日, 日本語, 口頭発表（一般）
千葉県千葉市, 日本国, ［国内会議］ - Conservation in botanic gardens in Japan
Koh Nakamura
Pusat Riset Konservasi Tumbuhan, Kebun Raya dan Kehutanan, BRIN. Garden Talk Series 1, Research and Conservation of Heath Forest in Botanical Gardens, 2023年02月14日, 英語, 口頭発表（招待・特別）
Bogor, Indonesia, インドネシア共和国, 42228908, ［招待講演］, ［国際会議］ - Cycas taitungensis C. F. Chen as a synonym of C. revoluta Thumb.
Jui-Tse Chang, Chien-Ti Chao, Koh Nakamura, Hsiao-Lei Liu, Min-Xin Luo, Pei-Chun Liao
The 3rd International Electronic Conference on Forests — Exploring New Discoveries and New Directions in Forests session Forest Ecophysiology and Genetics, 2022年10月31日, 英語, 口頭発表（一般）
Online, ［国際会議］, ［国際共著］ - 日本産イワベンケイ属の自生地と栽培条件下におけるフェノール成分量の変動
平野日向, 伊東拓朗, 中村剛, 村井良徳
日本植物学会第86回大会, 2022年09月15日, 日本語, ポスター発表
京都府京都市, 日本国, ［国内会議］ - 日本の遺存固有種オサバグサを含むケシ科の系統関係の推定
真野温人, Evgeny Boltenkov, Elena Marchuk, 中村剛, 渡辺洋一
日本植物学会第86回大会, 2022年09月15日, 日本語, ポスター発表
京都府京都市, 日本国, ［国内会議］, ［国際共著］ - 日本の植物園における生息域外保全の質的課題－複数園協働で種内の複数系統を保全するメタコレクションを目指して－
浅野(田村)紗彩, 遊川知久, 中村剛
日本植物園協会第57回大会, 2022年05月18日, 日本語, 口頭発表（一般）
岐阜県各務原市, 日本国, ［国内会議］ - タテヤマイワブキ（ユキノシタ科）の染色体数と系統的位置の推定
福田知子, 足立敏文, 中村剛
日本植物分類学会第 21 回大会, 2022年03月06日, 日本語, 口頭発表（一般）
オンライン, 日本国, ［国内会議］ - 植物園の導入・分譲履歴調査から明らかになった日本の域外保全の質的課題
田村紗彩, 遊川知久, 中村剛
日本植物分類学会第 21 回大会, 2022年03月05日, 日本語, ポスター発表
オンライン, 日本国, ［国内会議］ - 北海道大学植物園温室のマイナーリニューアル
大野祥子, 持田大, 永谷工, 高田純子, 東隆行, 中村剛
日本植物園協会第56回大会, 2021年06月, 日本語, ポスター発表
オンライン, 日本国, ［国内会議］ - Parentage of hybrids in a disturbed population of Betula ovalifolia endangered in Japan and suggestions for conservation management.
Yuki Shiotani, Tomoko Fukuda, Elena A. Marchuk, Pavel V. Krestov, Ekaterina A. Petrunenko, Svetlana N. Bondarchuk, Yoko Nishikawa, Takashi Shimamura, Yoshiyasu Fujimura, Koh Nakamura
日本植物分類学会第20回大会, 2021年03月 - ⼤陸辺縁の島弧が促す系統の分岐と再接触 –環⽇本海の南北2ルート移⼊とその意義–
中村剛
日本から発信する島嶼生物学―世界の島嶼生物学で日本列島が果たしうる役割を考える―, 2021年01月
［招待講演］ - 近隣国との互恵的協力による，北海道-東北アジアの希少植物の保全
中村剛
第6回 北大・部局横断シンポジウム, 2020年10月
共同研究・競争的資金等の研究課題
- 日露中韓共通の絶滅危惧植物の適応可能性を最大化する，東北アジアスケール保全単位
科学研究費助成事業
2024年04月01日 - 2028年03月31日
中村 剛, 國府方 吾郎, 福田 知子, 村井 良徳, 伊東 拓朗
日本学術振興会, 基盤研究(B), 北海道大学, 24K01773 - 琉球低地と台湾高地に産する植物の分布成立過程の追跡と環境適応の解明
科学研究費助成事業
2024年04月 - 2028年03月
國府方 吾郎, 傳田 哲郎, 中村 剛, 奥山 雄大, 伊東 拓朗
日本学術振興会, 基盤研究(B), 独立行政法人国立科学博物館, 24K02091 - 植物特性評価に基づいてブリトゥン島の荒野林を効果・効率的に保全する
科学研究費助成事業
2023年09月 - 2028年03月
國府方吾郎, 中村剛, 堤千絵, 伊東拓朗
日本学術振興会, 国際共同研究加速基金(海外連携研究), 独立行政法人国立科学博物館, 23KK0115 - 北海道固有の石灰岩植物シリベシナズナの遺伝的多様性を守る生息域外保全事業
生物多様性保全推進支援事業
2022年04月 - 2025年03月
中村剛
環境省, 研究代表者 - 東北アジアのアクセス困難地域の調査による，北海道希少植物の固有性と保全単位の 検証
科学研究費助成事業
2020年04月 - 2024年03月
中村剛, 村井良徳, 福田知子
北海道のヤチカンバ（環境省IB類）について，昨年度の研究（Shiotani et al. 2020）で固有種説を否定し東北アジア広域種と結論したことを受け，その保全価値を氷期遺存集団という点と日本の遺伝資源という点で再評価し，保全遺伝解析を行った．自生地の湿原では排水路の乾燥環境にヤチカンバの雑種個体が確認される．遺伝解析の結果，雑種片親は排水路に生育するシラカンバではなく，近隣には生育しないダケカンバと判明した．日中露サンプルに基づきヤチカンバの種特異遺伝マーカーを開発して雑種判別を行い，遺伝的汚染の防止を議論した（Shiotani et al., 2022）．
ユウバリクモマグサ（環境省IA類）は，シコタンソウ（本州，北海道，南千島，サハリン）とは別種で，北海道固有であることを我々は示したが（Tamura et al., 2018），その学名を担うホロタイプが所在不明であった．分類・保全の基礎である学名の不備を解決するため，主要標本庫を精査したがホロタイプは認められないことから，命名規約に従い原記載の図解をレクトタイプに指定した（Tamura et al., 2022）．
周北極分布するシベリアイワブキとその2変種，チシマイワブキ（北海道．環境省IB類），タテヤマイワブキ（北アルプス．長野I類，富山II類）の系統関係を染色体・遺伝データから推定した（Fukuda et al., 査読中）．チシマイワブキはサハリン・大陸ロシア系統と千島・アリューシャン系統の交雑で，また，タテヤマイワブキは千島・アリューシャン系統とクロクモソウ系統の交雑で生じた可能性が示された．両変種は保全上，区別すべきといえる．
さらに，北海道，つまり国境域の絶滅危惧植物の保全における固有性，保全単位，国際協力の問題，生息域外保全の課題と将来ビジョンなどについて議論した（中村・田村, 2022）．
科研費基盤研究C, 基盤研究(C), 北海道大学, 研究代表者, 20K06087 - 北海道等のカラフトグワイの遺伝的多様性を守る生息域外保全事業
生物多様性保全推進支援事業
2019年04月 - 2022年03月
中村剛
環境省, 研究代表者, 競争的資金 - 北海道崕山固有キリギシソウの遺伝的多型を守る生息域外保全事業
生物多様性保全推進支援事業
2018年04月 - 2021年03月
中村剛
環境省, 研究代表者, 競争的資金 - 極東ロシアとの比較による，北海道指定希少植物の固有性，集団分化の検証と保全提言
2016年04月 - 2020年03月
中村剛
科研費若手研究B, 研究代表者, 競争的資金 - 国境を越えて分布する北海道指定希少野生植物をモデルとした，国際共同保全システムの確立
2016年04月 - 2019年03月
中村剛
三井物産環境基金研究助成, 研究代表者, 競争的資金 - ユウバリクモマグサの生息域外保全のための生育地調査及び種子等採集活動
環境省希少野生植物の生息域外保全検討実施委託業務
2019年 - 2019年
中村剛
研究代表者, 競争的資金 - オニオトコヨモギの生息域外保全のための生育地調査及び種子等採集活動
2018年 - 2018年
中村剛
環境省希少野生植物の生息域外保全検討実施委託業務, 研究代表者, 競争的資金 - 北海道の自然を特徴づける湿地植物の保全遺伝学的研究
2015年04月 - 2016年03月
中村剛
公益財団法人・栗林育英学術財団研究助成, 研究代表者, 競争的資金 - 博士研究員研究奨励費
2014年01月 - 2015年03月
中村剛
中華民国科技部, 研究代表者, 競争的資金 - 招聘博士研究員研究奨励費
2012年01月 - 2013年12月
中村剛
台湾中央研究院 生命科学組, 研究代表者, 競争的資金 - 招聘博士研究員研究奨励費
2010年02月 - 2011年12月
中村剛
台湾中央研究院 生命科学組, 研究代表者, 競争的資金 - 琉球列島とフィリピン，オーストラリアに隔離分布する植物の系統地理学的解析
2008年04月 - 2009年03月
中村剛
琉球大学21世紀COEプログラム公募研究費, 研究代表者, 競争的資金 - 琉球列島産植物の系統地理パタンにおける，地史的，生態学的要因に関する研究
2007年04月 - 2008年03月
中村剛
琉球大学21世紀COEプログラム公募研究費, 研究代表者, 競争的資金 - 琉球列島産陸上維管束植物の系統地理学的研究
科学研究費助成事業
2004年04月 - 2007年03月
中村剛
琉球列島は,大陸東縁の陸橋に由来し,鮮新世末から更新世初期にまず北,中,南琉球が分断され,漸次現在の島弧が成立したと考えられている.この島嶼化の過程が,植物相の分化や,植物種内の遺伝的分化に与えた影響を調査した.
琉球列島の主要26島について,種子植物ほぼ全種約1800種の有無を文献調査し,島間の植物相の類似度を種,属,科のレベルで算出した.この類似度に基づく解析から,琉球列島の植物相は,種および属レベルで見た場合に,北,中,南琉球間で有意に異なることが示された.
草本種サツマイナモリの系統地理学的解析を葉緑体DNA塩基配列に基づき行なった.その結果,琉球列島の複数の遺伝的タイプおよび中琉球固有の四倍体変種アマミイナモリはいずれも,台湾以南で既に分化し,その後に琉球列島へ進入したことが示唆された.また,トカラ海峡を挟む北,中琉球間で,遺伝的分化は認められなかった.この結果は,琉球列島の生物の分化が,海峡成立などにより当地域内で引き起こされたとする従来の説に対する重要な反例であり,今後の当地域の生物地理学的研究に大きな影響を与える示唆をもたらしている.また,系統地理学的研究においては独創的な試みとして,低温耐性の種内多型の有無を光合成活性を指標に調査した.その結果,低緯度(南琉球)の集団は高緯度(中琉球から九州)の集団に比べ,低温耐性が低いことが示された.このことは,琉球列島を分布北上する過程で,種内の一部は,緯度上昇に伴う気温低下により分散に制限を受けた可能性を示唆した.
また,アマミイナモリについては,分子系統解析の結果,単系統群として明瞭に区別されたこと,さらに,サツマイナモリに見られる二型花柱性がアマミイナモリでは崩壊しており繁殖様式にも分化が認められたことから,その分類学的取り扱いを独立種とすることを提案した(日本植物分類学会第6回大会大会発表賞(口頭発表部門)受賞).
科研費特別研究員奨励費, 特別研究員奨励費, 琉球大学, 研究代表者, 競争的資金, 04J54231
