Jul. 2025, Royal Society of Chemistry, Nanoscale Advances Paper Prize Award　　　　　　　　　　　　　　　
Runner-Up Paper
Masatoshi Maeki;Niko Kimura;Yuto Okada;Kazuki Shimizu;Kana Shibata;Yusuke Miyazaki;Akihiko Ishida;Kento Yonezawa;Nobutaka Shimizu;Wataru Shinoda;Manabu Tokeshi

Oct. 2021, Network Joint Research Center for Materials and Devices, Material / Device Joint Research Award　　　　　　　　　　　　　　　
蛍光偏光イムノアッセイ法による抗体検出法
Manabu Tokeshi;Keine Nishiyama;Kazuki Takahashi;Akihide Hibara

Feb. 2021, 北海道大学, 教育研究総長表彰　　　　　　　　　　　　　　　
渡慶次学

Apr. 2019, Royal Society of Chemistry, Fellow（FRSC）　　　　　　　　　　　　　　　
TOKESHI Manabu

Sep. 2018, 日本分析化学会, 学会賞　　　　　　　　　　　　　　　
渡慶次 学

Oct. 2011, 堀場製作所, 堀場雅夫賞　　　　　　　　　　　　　　　
渡慶次 学

Oct. 2007, Pioneers of Miniaturisation Prize　　　　　　　　　　　　　　　
Japan

Jul. 2007, 可視化情報学会学会賞（論文賞）　　　　　　　　　　　　　　　
Japan

May 2007, CHEMINAS奨励賞　　　　　　　　　　　　　　　
Japan

Examining the Impact of Storage Conditions on the Stability of a Liquid Formulation of mRNA-Loaded Lipid Nanoparticles
Mina Sato, Eleni Samaridou, Moritz Beck-Broichsitter, Masatoshi Maeki, Shunsuke Kita, Manabu Tokeshi, Katsumi Maenaka, Hideyoshi Harashima, Yusuke Sato
Pharmaceutics, 17, 9, 1194, 1194, MDPI AG, 14 Sep. 2025, [Peer-reviewed]
English, Scientific journal, Background/Objectives: This study investigated the effect of storage conditions on mRNA-LNPs in situ via identification of the formulation traits necessary for improving storage stability. Methods: We synthesized an ionizable lipid, namely TOT-28, which has a hydrolysis-susceptible ester bond in its hydrophilic head group that allows it to act as an indicator of the hydrophilic environment within the mRNA-LNPs. LNPs were stored either at 4 or 25 °C for up to 8 weeks to investigate the effect of pH and temperature on ester hydrolysis, internal mRNA integrity, physicochemical properties of the LNPs, and mRNA gene expression. Results: The results indicate that, at 25 °C, a lower buffer pH increases ester hydrolysis, whereas an opposite trend slightly occurs in ester hydrolysis with storage at 4 °C. We also found that TOT-28-based LNPs were less hydrated and microviscosity was higher at 4 °C compared with storage temperature at 25 °C. Therefore, TOT-28-based LNPs seem less sensitive to external buffer solutions because of a higher-order structure when stored at lower temperatures. In addition, we found that LNPs with different ionizable lipid structures exhibit distinct responses to pH changes at specific storage temperatures. Conclusions: Our findings provide novel insights into the appropriate conditions for long-term storage of the mRNA-LNPs as a liquid formulation.

Development of Paper-based Microfluidic Devices for the Detection of Cannabis Components
Akihiko Ishida, Nozomi Nagase, Chikako Takei, Kayo Nakamura, Hideyo Takahashi, Hiroko Abe, Manabu Tokeshi
IEEJ Transactions on Sensors and Micromachines, 145, 9, 195, 200, Institute of Electrical Engineers of Japan (IEE Japan), 01 Sep. 2025, [Peer-reviewed], [Last author]
Japanese, Scientific journal, 50144367

Paper discs in a 3D printed microplate hybrid microfluidic device for low-cost, rapid, and ultrasensitive paper-based bioluminescence detection of human epidermal growth factor receptor 2 (HER2) breast cancer biomarker
Ahmed A. Shalaby, Asmaa Salah, Akihiko Ishida, Masatoshi Maeki, Manabu Tokeshi
Biosensors and Bioelectronics: X, 24, 100621, 100621, Elsevier BV, Aug. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Isolating and Determining the Structures of Colored Products from the Reactions of Cannabinoids with Fast Blue RR
Kayo Nakamura, Hikari Nishiguchi, Ryosuke Arai, Riho Hamajima, Hiroko Abe, Akihiko Ishida, Manabu Tokeshi, Kyohei Higashi, Akiyoshi Saitoh, Hideyo Takahashi
Molecules, 30, 3462, Aug. 2025, [Peer-reviewed]
English, Scientific journal

Lipid nanoparticle delivery of the CRISPR/Cas9 system directly into the mitochondria of cells carrying m.7778G>T mutation in MtDNA (mt-Atp8)
Kaede Norota, Sen Ishizuka, Misa Hirose, Yusuke Sato, Masatoshi Maeki, Manabu Tokeshi, Saleh M. Ibrahim, Hideyoshi Harashima, Yuma Yamada
Scientific Reports, 15, 1, Springer Science and Business Media LLC, 19 Jun. 2025, [Peer-reviewed]
English, Scientific journal

Microfluidic Production of Exosome-Mimicking Lipid Nanoparticles for Enhanced RNA Delivery: Role of Exosomal Proteins
Masatoshi Maeki, Ayuka Niwa, Shota Oyama, Kyoko Aratani, Rina Ito, Yuichi Suzuki, Yusuke Sato, Akihiko Ishida, Hideyoshi Harashima, Manabu Tokeshi
ACS Applied Materials & Interfaces, 17, 29, 41666, 41679, American Chemical Society (ACS), 16 Jun. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Conjugated Polymer Nanoparticles and Thin Films of Defect-Free Cyclic P3HT: Effects of Polymer Topology on the Nanostructure
Tomohisa Watanabe, Masatoshi Maeki, Manabu Tokeshi, Tianle Gao, Feng Li, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Takuya Yamamoto
Molecules, 30, 2490, Jun. 2025, [Peer-reviewed]
English, Scientific journal

Unraveling the Molecular Mechanism of Transient Multilamellar Formation in Ethanol-Modified Vesicle Solutions
Kana Shibata, Masatoshi Maeki, Manabu Tokeshi, Wataru Shinoda
Langmuir, 41, 21, 13372, 13380, American Chemical Society (ACS), 20 May 2025, [Peer-reviewed]
English, Scientific journal

Microfluidic technologies for protein crystallography: advances and applications
Masatoshi Maeki, Akihiko Ishida, Manabu Tokeshi
Analytical Sciences, 41, 8, 1203, 1211, Springer Science and Business Media LLC, 21 Apr. 2025, [Peer-reviewed], [Invited], [Last author]
English, Scientific journal

Microfluidic paper-based analytical devices: proven applications and future prospects in therapeutic drug monitoring, homeland security, and chemical education
Manabu Tokeshi
Analytical Sciences, 41, 8, 1157, 1168, Springer Science and Business Media LLC, 19 Apr. 2025, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
English, Scientific journal, 45205131

Splenic B cell-targeting lipid nanoparticles for safe and effective mRNA vaccine delivery.
Yuichi Suzuki, Mai Yakuwa, Mina Sato, Eleni Samaridou, Moritz Beck-Broichsitter, Masatoshi Maeki, Manabu Tokeshi, Yuma Yamada, Hideyoshi Harashima, Yusuke Sato
Journal of Controlled Release, 382, 113687, 113687, 03 Apr. 2025, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, mRNA-loaded lipid nanoparticles (LNPs) have emerged as a potent and versatile platform that underpins the success of mRNA vaccines, but guidelines for designing safe and effective formulations with minimal off-target effects remain unclear. In this study, we focused on a rational design for a novel ionizable lipid library that is based on ionizable tri-oleoyl-tris (iTOT) compounds with a high yield via a simple 2-step synthesis. To further enhance the efficacy and safety of this potent library for vaccine applications, we identified the optimal composition for a vaccine by focusing on the molar ratio of specific lipid excipients in the formulation. This composition brought about a shift in delivery to the spleen, and the LNP formulation, which contained 15 mol% DSPC (15%DSPC-LNPs), was thoroughly taken up by both B cells and other splenic immune cells. This formulation requires neither additional lipid components nor targeting ligand modifications, and it is accompanied by antigen-specific cytotoxic T lymphocyte responses. The rigid, hydrophobic, and charge-neutral surface of 15%DSPC-LNPs minimizes apolipoprotein E-dependent hepatic uptake and maximizes complement receptor-mediated B-cell targeting. Furthermore, as an intramuscularly administered vaccine, 15%DSPC-LNPs induce antigen-specific immune responses and, importantly, results in significantly lower levels of hepatotoxicity compared with that of the mRNA vaccine formulations currently being marketed. In summary, this study demonstrated how the passive targeting of mRNA-LNPs to organs and cells could be regulated by designing novel ionizable lipids combined with adjusting the relative proportions of lipid components. The results of this study also emphasize how selective mRNA delivery to the spleen could avoid the liver, which highlights a promising strategy for the development of safe and effective vaccines.

2-Propanol Suspension Method to Increase Acetylcholinesterase and Flow Stability on μPADs
Akinori Yamaguchi, Shota Oyama, Akihiko Ishida, Takanori Enomoto, Nobuyuki Sanari, Hajime Miyaguchi, Manabu Tokeshi
ACS Applied Bio Materials, 8, 2, 1699, 1706, American Chemical Society (ACS), 24 Jan. 2025, [Peer-reviewed], [Last author]
English, Scientific journal

Marginal-zone B cells as promising targets of an mRNA-loaded, lipid-nanoparticle cancer vaccine
Yuichi Suzuki, Mai Yakuwa, Mina Sato, Eleni Samaridou, Moritz Beck-Broichsitter, Masatoshi Maeki, Manabu Tokeshi, Yuma Yamada, Hideyoshi Harashima, Yusuke Sato
Next Nanotechnology, 8, 100154, 100154, Elsevier BV, 2025, [Peer-reviewed]
English, Scientific journal

Straightforward high-temperature antibody immobilization for rapid and simple microfluidic paper-based ELISA for detection of β’-component (Onc k 5) protein, a major IgE-binding protein in salmon roe
Ahmed A. Shalaby, Ryoga Maeda, Akihiko Ishida, Yutaka Shimizu, Hiroki Saeki, Masatoshi Maeki, Manabu Tokeshi
Sensors and Actuators B: Chemical, 422, 01 Jan. 2025, [Peer-reviewed], [Invited], [Last author, Corresponding author]
Scientific journal

Enhanced Broad-Spectrum Efficacy of an L2-Based mRNA Vaccine Targeting HPV Types 6, 11, 16, 18, with Cross-Protection Against Multiple Additional High-Risk Types
Kosuke Tsukamoto, Akio Yamashita, Masatoshi Maeki, Manabu Tokeshi, Hirotatsu Imai, Akira Fukao, Toshinobu Fujiwara, Koji Okudera, Nobuhisa Mizuki, Kenji Okuda, Masaru Shimada
Vaccines, Oct. 2024, [Peer-reviewed]
Scientific journal

Investigation of the Nanoparticulation Method and Cell-Killing Effect following the Mitochondrial Delivery of Hydrophobic Porphyrin-Based Photosensitizers
Rina Naganawa, Hanjun Zhao, Yuta Takano, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima, Yuma Yamada
International Journal of Molecular Sciences, 25, 8, 4294, 4294, MDPI AG, 12 Apr. 2024, [Peer-reviewed]
English, Scientific journal, Photodynamic therapy is expected to be a less invasive treatment, and strategies for targeting mitochondria, the main sources of singlet oxygen, are attracting attention to increase the efficacy of photodynamic therapy and reduce its side effects. To date, we have succeeded in encapsulating the photosensitizer rTPA into MITO-Porter (MP), a mitochondria-targeted Drug Delivery System (DDS), aimed at mitochondrial delivery of the photosensitizer while maintaining its activity. In this study, we report the results of our studies to alleviate rTPA aggregation in an effort to improve drug efficacy and assess the usefulness of modifying the rTPA side chain to improve the mitochondrial retention of MITO-Porter, which exhibits high therapeutic efficacy. Conventional rTPA with anionic side chains and two rTPA analogs with side chains that were converted to neutral or cationic side chains were encapsulated into MITO-Porter. Low-MP (MITO-Porter with Low Drug/Lipid) exhibited high drug efficacy for all three types of rTPA, and in Low-MP, charged rTPA-encapsulated MP exhibited high drug efficacy. The cellular uptake and mitochondrial translocation capacities were similar for all particles, suggesting that differences in aggregation rates during the incorporation of rTPA into MITO-Porter resulted in differences in drug efficacy.

Discrimination of extracellular miRNA sources for the identification of tumor-related functions based on nanowire thermofluidics
Kunanon Chattrairat, Akira Yokoi, Min Zhang, Mikiko Iida, Kosuke Yoshida, Masami Kitagawa, Ayuka Niwa, Masatoshi Maeki, Takeshi Hasegawa, Takeshi Yokoyama, Yoshikazu Tanaka, Yusuke Miyazaki, Wataru Shinoda, Manabu Tokeshi, Kazuki Nagashima, Takeshi Yanagida, Hiroaki Kajiyama, Yoshinobu Baba, Takao Yasui
Device, 2, 6, 100363, 100363, Elsevier BV, Apr. 2024, [Peer-reviewed]
English, Scientific journal

Development of Polymer-Lipid Hybrid Nanoparticles for Large-Sized Plasmid DNA Transfection.
Masatoshi Maeki, Shuya Uno, Kaisei Sugiura, Yusuke Sato, Yoichiro Fujioka, Akihiko Ishida, Yusuke Ohba, Hideyoshi Harashima, Manabu Tokeshi
ACS Applied Materials & Interfaces, 16, 2, 2110, 2119, 17 Jan. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, RNA and DNA delivery technologies using lipid nanoparticles (LNPs) have advanced significantly, as demonstrated by their successful application in mRNA vaccines. To date, commercially available RNA therapeutics include Onpattro, a 21 bp siRNA, and mRNA vaccines comprising 4300 nucleotides for COVID-19. However, a significant challenge remains in achieving efficient transfection, as the size of the delivered RNA and DNA increases. In contrast to RNA transfection, plasmid DNA (pDNA) transfection requires multiple steps, including cellular uptake, endosomal escape, nuclear translocation, transcription, and translation. The low transfection efficiency of large pDNA is a critical limitation in the development of artificial cells and their cellular functionalization. Here, we introduce polymer-lipid hybrid nanoparticles designed for efficient, large-sized pDNA transfection. We demonstrated that LNPs loaded with positively charged pDNA-polycation core nanoparticles exhibited a 4-fold increase in transfection efficiency for 15 kbp pDNA compared with conventional LNPs, which encapsulate a negatively charged pDNA-polycation core. Based on assessments of the size and internal structure of the polymer-lipid nanoparticles as well as hemolysis and cellular uptake analysis, we propose a strategy to enhance large-sized pDNA transfection using LNPs. This approach holds promise for accelerating the in vivo delivery of large-sized pDNA and advancing the development of artificial cells.

Sensitivity-improved blocking agent-free fluorescence polarization assay through surface modification using polyethylene glycol
Hao Liu, Mao Fukuyama, Yu Ogura, Motohiro Kasuya, Sho Onose, Ayuko Imai, Koji Shigemura, Manabu Tokeshi, Akihide Hibara
The Analyst, 149, 20, 5139, 5144, Royal Society of Chemistry (RSC), 2024, [Peer-reviewed]
English, Scientific journal, A polyethylene glycol-based surface modification improved the sensitivity of fluorescence polarization assays in microfluidic devices, eliminating the need for blocking agents.

Understanding the effects of ethanol on the liposome bilayer structure using microfluidic-based time-resolved small-angle X-ray scattering and molecular dynamics simulations
Masatoshi Maeki, Niko Kimura, Yuto Okada, Kazuki Shimizu, Kana Shibata, Yusuke Miyazaki, Akihiko Ishida, Kento Yonezawa, Nobutaka Shimizu, Wataru Shinoda, Manabu Tokeshi
Nanoscale Advances, 6, 8, 2166, 2176, Royal Society of Chemistry (RSC), 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, We investigated ethanol-induced structural changes in liposomes on a time scale from microseconds to tens of seconds using a microfluidic-based small-angle X-ray scattering (SAXS) measurement system coupled with molecular dynamics (MD) simulations.

An Ionizable Lipid Material with a Vitamin E Scaffold as an mRNA Vaccine Platform for Efficient Cytotoxic T Cell Responses
Ryotaro Oyama, Harumichi Ishigame, Hiroki Tanaka, Naho Tateshita, Moeko Itazawa, Ryosuke Imai, Naomasa Nishiumi, Jun-ichi Kishikawa, Takayuki Kato, Jessica Anindita, Yoshifumi Nishikawa, Masatoshi Maeki, Manabu Tokeshi, Kota Tange, Yuta Nakai, Yu Sakurai, Takaharu Okada, Hidetaka Akita
ACS Nano, 17, 19, 18758, 18774, American Chemical Society (ACS), 26 Sep. 2023
English, Scientific journal

A portable liquid chromatography system based on a separation/detection chip module consisting of a replaceable ultraviolet-visible absorbance or contactless conductivity detection unit
Akihiko Ishida, Takuma Nishimura, Kaito Koyama, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
Journal of Chromatography A, 1706, 464272, 464272, Elsevier BV, Sep. 2023, [Peer-reviewed], [Last author]
Scientific journal

Rapid determination of domoic acid in seafood by fluorescence polarization immunoassay using a portable analyzer
Yu Ogura, Mao Fukuyama, Motohiro Kasuya, Koji Shigemura, Sergei A. Eremin, Manabu Tokeshi, Akihide Hibara
Analytical Sciences, 149, 20, 5139, 5144, Springer Science and Business Media LLC, 31 Aug. 2023, [Peer-reviewed]
Scientific journal, Abstract

Monitoring phycotoxin accumulation in marine products such as edible shellfish is a regulatory requirement in many countries. Therefore, a simple and rapid onsite quantification method is sought. Herein, we present a fluorescence polarization immunoassay (FPIA), a well-known one-step immunoassay, using a portable fluorescence polarization analyzer for domoic acid (DA), widely referred to as the primary toxin of amnesic shellfish poisoning (ASP). To establish FPIA for DA, the matrix effect of methanol, which is widely used to extract DA from shellfish, on FPIA was investigated. To validate this method, we performed a spike recovery test using oysters containing DA at a concentration equivalent to the regulatory limits of North America and the European Union (20 mg/kg). The recovery rate was found to be 79.4–114.7%, which is equivalent to that of the commercially available enzyme-linked immunosorbent assay (ELISA). We expect that this FPIA system will enable the quantitative onsite analysis of DA and significantly contribute to the safety of marine products.

Graphical Abstract

Micro- and nanochamber array system for single enzyme assays.
Kazuki Iijima, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Scientific reports, 13, 1, 13322, 13322, 16 Aug. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Arrays of small reaction containers, ranging from 624 femtoliters (10-15 L) to 270 attoliters (10-18 L), for capturing a single enzyme molecule and measuring the activity were developed along with a new reversible sealing system based on a pneumatic valve actuator made of polydimethylsiloxane (PDMS). The valve was actuated by PBS solution, effectively preventing evaporation of the solution from the micro- and nanochambers and allowing the assay to be performed over a long period of time. The hydrolysis rates of β-D-galactosidase (β-gal), kcat, were decreased according to the decrease of the chamber size, and the overall tendency seems to be symmetrically related to the specific surface area of the chambers even under the prevented condition of non-specific adsorption. The spatial localization of the protons in the chambers, which might could affect the dissociation state of the proteins, was also investigated to explain the decrease in the hydrolysis rate. The developed chamber system developed here may be useful for artificially reproducing the confined intracellular environment and molecular crowding conditions.

Structure of lasso peptide epimerase MslH reveals metal-dependent acid/base catalytic mechanism
Yu Nakashima, Atsushi Kawakami, Yasushi Ogasawara, Masatoshi Maeki, Manabu Tokeshi, Tohru Dairi, Hiroyuki Morita
Nature Communications, 14, 1, Springer Science and Business Media LLC, 08 Aug. 2023, [Peer-reviewed]
English, Scientific journal, Abstract

The lasso peptide MS-271 is a ribosomally synthesized and post-translationally modified peptide (RiPP) consisting of 21 amino acids with D-tryptophan at the C-terminus, and is derived from the precursor peptide MslA. MslH, encoded in the MS-271 biosynthetic gene cluster (msl), catalyzes the epimerization at the Cα center of the MslA C-terminal Trp21, leading to epi-MslA. The detailed catalytic process, including the catalytic site and cofactors, has remained enigmatic. Herein, based on X-ray crystallographic studies in association with MslA core peptide analogues, we show that MslH is a metallo-dependent peptide epimerase with a calcineurin-like fold. The crystal structure analysis, followed by site-directed mutagenesis, docking simulation, and ICP-MS studies demonstrate that MslH employs acid/base chemistry to facilitate the reversible epimerization of the C-terminal Trp21 of MslA, by utilizing two pairs of His/Asp catalytic residues that are electrostatically tethered to a six-coordination motif with a Ca(II) ion via water molecules.

Construction of the systemic anticancer immune environment in tumour-bearing humanized mouse by using liposome-encapsulated anti-programmed death ligand 1 antibody-conjugated progesterone
Yoshie Kametani, Ryoji Ito, Shino Ohshima, Yoshiyuki Manabe, Yusuke Ohno, Tomoka Shimizu, Soga Yamada, Nagi Katano, Daiki Kirigaya, Keita Ito, Takuya Matsumoto, Banri Tsuda, Hirofumi Kashiwagi, Yumiko Goto, Atsushi Yasuda, Masatoshi Maeki, Manabu Tokeshi, Toshiro Seki, Koichi Fukase, Mikio Mikami, Kiyoshi Ando, Hitoshi Ishimoto, Takashi Shiina
Frontiers in Immunology, 14, 1173728, Frontiers Media SA, 10 Jul. 2023, [Peer-reviewed]
English, Scientific journal

Antigen/Adjuvant-Displaying Enveloped Viral Replica as a Self-Adjuvanting Anti-Breast-Cancer Vaccine Candidate
Keita Ito, Hiroto Furukawa, Hiroshi Inaba, Shino Ohshima, Yoshie Kametani, Masatoshi Maeki, Manabu Tokeshi, Xuhao Huang, Kazuya Kabayama, Yoshiyuki Manabe, Koichi Fukase, Kazunori Matsuura
Journal of the American Chemical Society, 145, 29, 15838, 15847, American Chemical Society (ACS), 21 Jun. 2023, [Peer-reviewed]
Scientific journal

A system that delivers an antioxidant to mitochondria for the treatment of drug-induced liver injury.
Mitsue Hibino, Masatoshi Maeki, Manabu Tokeshi, Yoichi Ishitsuka, Hideyoshi Harashima, Yuma Yamada
Scientific Reports, 13, 1, 6961, 6961, 10 May 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Mitochondria, a major source of reactive oxygen species (ROS), are intimately involved in the response to oxidative stress in the body. The production of excessive ROS affects the balance between oxidative responses and antioxidant defense mechanisms thus perturbing mitochondrial function eventually leading to tissue injury. Therefore, antioxidant therapies that target mitochondria can be used to treat such diseases and improve general health. This study reports on an attempt to establish a system for delivering an antioxidant molecule coenzyme Q10 (CoQ10) to mitochondria and the validation of its therapeutic efficacy in a model of acetaminophen (APAP) liver injury caused by oxidative stress in mitochondria. A CoQ10-MITO-Porter, a mitochondrial targeting lipid nanoparticle (LNP) containing encapsulated CoQ10, was prepared using a microfluidic device. It was essential to include polyethylene glycol (PEG) in the lipid composition of this LNP to ensure stability of the CoQ10, since it is relatively insoluble in water. Based on transmission electron microscope (TEM) observations and small angle X-ray scattering (SAXS) measurements, the CoQ10-MITO-Porter was estimated to be a 50 nm spherical particle without a regular layer structure. The use of the CoQ10-MITO-Porter improved liver function and reduced tissue injury, suggesting that it exerted a therapeutic effect on APAP liver injury.

Mass production system for RNA-loaded lipid nanoparticles using piling up microfluidic devices
Masatoshi Maeki, Yuto Okada, Shuya Uno, Kaisei Sugiura, Yuichi Suzuki, Kento Okuda, Yusuke Sato, Masao Ando, Hiroyuki Yamazaki, Masaki Takeuchi, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
Applied Materials Today, 31, 101754, 101754, Elsevier BV, Apr. 2023, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Microfluidic paper-based analytical devices for cancer diagnosis
Ahmed A. Shalaby, Chia Wen Tsao, Akihiko Ishida, Masatoshi Maeki, Manabu Tokeshi
Sensors and Actuators B: Chemical, 379, 133243, 133243, Elsevier BV, 15 Mar. 2023, [Peer-reviewed], [Invited], [Last author, Corresponding author]
English, Scientific journal, 45205131

Effects of Addition of Blocking Agents on Fluorescence Polarization Immunoassay of Okadaic Acid Using a Microfluidic Device
Shunsuke CHIDA, Kazuki TAKAHASHI, Mao FUKUYAMA, Motohiro KASUYA, Masatoshi MAEKI, Akihiko ISHIDA, Hirofumi TANI, Koji SHIGEMURA, Anatoly V. ZHERDEV, Sergei A. EREMIN, Akihide HIBARA, Manabu TOKESHI
BUNSEKI KAGAKU, 72, 3, 133, 138, Japan Society for Analytical Chemistry, 05 Mar. 2023, [Peer-reviewed], [Last author, Corresponding author]
Japanese, Scientific journal

Development of an absorbance detection module using a deep UV light-emitting diode for a portable miniaturized liquid chromatograph
Kaito Koyama, Takuma Nishimura, Akihiko Ishida, Mitsue Hibino, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
BUNSEKI KAGAKU, 72, 3, 125, 131, Mar. 2023, [Peer-reviewed], [Last author]
Japanese, Scientific journal

Controlling lamellarity and physicochemical properties of liposomes prepared using a microfluidic device
Yuka Matsuura-Sawada, Masatoshi Maeki, Shuya Uno, Koichi Wada, Manabu Tokeshi
Biomaterials Science, 11, 7, 2419, 2426, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Microfluidic Platform Enabling Efficient On-Device Preparation of Lipid Nanoparticles for Formulation Screening
Yuka Matsuura-Sawada, Shuya Uno, Masatoshi Maeki, Koichi Wada, Manabu Tokeshi
ACS Applied Engineering Materials, 1, 1, 278, 286, American Chemical Society (ACS), 10 Nov. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Effect of Organic Solvents on a Production of PLGA-Based Drug-Loaded Nanoparticles Using a Microfluidic Device
Yi Bao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Omega, 7, 37, 33079, 33086, 20 Sep. 2022, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Fine-tuning the encapsulation of a photosensitizer in nanoparticles reveals the relationship between internal structure and phototherapeutic effects.
Fumika Kubota, Satrialdi, Yuta Takano, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima, Yuma Yamada
Journal of Biophotonics, 16, 3, e202200119, 01 Sep. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Photodynamic therapy (PDT) is a cancer therapy that uses a photosensitizer (PS) in the presence of oxygen molecules. Since singlet oxygen is highly reactive, it is important to deliver it to the target site. Thus, an efficient drug delivery system (DDS) is essential for enhancing the efficacy of such a treatment and protecting against the side effects of PDT. Here, we report on attempts to increase the therapeutic effect of PDT by using a DDS, a lipid nanoparticle (LNP). We prepared a porphyrin analog, rTPA (PS) that was encapsulated in LNPs using a microfluidic device. The findings indicated that the internal structure of the prepared particles changed depending on the amount of rTPA in LNPs. The photoactivity and cell-killing effect of PS in LNPs also changed when the amount of the cargo increased. These results suggest that the internal structure of LNPs is important factors that affect drug efficacy. This article is protected by copyright. All rights reserved.

Preparation of size-tunable sub-200 nm PLGA-based nanoparticles with a wide size range using a microfluidic platform
Yi Bao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
PLOS ONE, 17, 8, Aug. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

On the size-regulation of RNA-loaded lipid nanoparticles synthesized by microfluidic device.
Kento Okuda, Yusuke Sato, Kazuki Iwakawa, Kosuke Sasaki, Nana Okabe, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima
Journal of Controlled Release, 348, 648, 659, 18 Jun. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, The use of lipid nanoparticles (LNPs) for nucleic acid delivery is now becoming a promising strategy with a number of clinical trials as vaccines or as novel therapies against a variety of genetic and infectious diseases. The use of microfluidics for the synthesis of the LNPs has attracted interest because of its considerable advantages over other conventional synthetic methods including scalability, reproducibility, and speed. However, despite the potential usefulness of large particles for nucleic acid delivery to dendritic cells (DCs) as a vaccine, the particle size of the LNPs prepared using microfluidics is typically limited to approximately from 30 to 100 nm. In this study, focusing on Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, the effect of some synthetic parameters, including total flow rate, flow rate ratio, buffer pH, lipid concentration, molar ratio of PEG-lipid as well as salt concentration, on particle size was systematically examined by means of the design of experiment approaches. The findings indicated that the simple addition of salt (e.g. NaCl) to a buffer containing nucleic acids contributed greatly to the synthesis of large LNPs over 200 nm and this effect was concentration-dependent with respect to the salt. The effect of salt on particle size was consistent with a Hofmeister series. The systemic injection of larger mRNA-loaded LNPs resulted in a higher transgene expression in mouse splenic DCs, a higher activation of various splenic immune cells, and had a superior effect as a therapeutic cancer vaccine in a syngeneic mouse model compared to the smaller-sized counterpart with constant lipid composition prepared with lower NaCl concentration. Collectively, size-regulation by the simple addition of salt is a promising strategy for developing potent LNPs.

Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer.
Kazuki Takahashi, Shunsuke Chida, Thanawat Suwatthanarak, Mikiko Iida, Min Zhang, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Takao Yasui, Yoshinobu Baba, Akihide Hibara, Mina Okochi, Manabu Tokeshi
Lab on a Chip, 22, 16, 2971, 2977, 17 Jun. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.

Microfluidic technologies and devices for lipid nanoparticle-based RNA delivery
Masatoshi Maeki, Shuya Uno, Ayuka Niwa, Yuto Okada, Manabu Tokeshi
Journal of Controlled Release, 344, 80, 96, Elsevier BV, Apr. 2022, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

Enzyme kinetics in confined geometries at the single enzyme level.
Hisashi Murahara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
The Analyst, 147, 7, 1375, 1384, 28 Mar. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, The effects of increased confinement on the catalytic rates of individual enzyme molecules were studied at the single molecule level using femtolitre chambers and molecular crowders. According to the increase of confinement, from micro to nanometer cubic space in the chambers, the hydrolysis rate of β-galactosidase (β-gal) decreased to one-tenth of the rate in bulk. When molecular crowders suppressed the diffusion rates that reduced the collision chance of an enzyme and a substrate, the hydrolysis rate also decreased, which happened also in the case of femtolitre chambers. However, their kinetic trend was different especially from the viewpoint of the diffusion rates in diffusion-limited space. These data suggested that cell or organelle-scale confined environments might affect the kinetics of biochemical reactions and emphasized the importance of understanding enzyme kinetics in the in vivo environment.

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device.
Masatoshi Maeki, Yuto Okada, Shuya Uno, Ayuka Niwa, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Journal of Visualized Experiments : JoVE, 2022, 181, 22 Mar. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal

Dimethoxytriadinylation LC-MS/MS of Novichok A-Series Degradation Products in Human Urine.
Akinori Yamaguchi, Hajime Miyaguchi, Manabu Tokeshi
Analytical Chemistry, 94, 11, 4658, 4665, 22 Mar. 2022, [Peer-reviewed], [Last author], [International Magazine]
English, Scientific journal, Novichok A-series compounds, novel nerve agents, pose an increasing threat to citizens worldwide; however, no analytical methods have been reported for detecting their hydrolysis products. Herein, a screening method was developed to detect and identify Novichok A-series degradation products (hydrolysates of A230, A232, A234, A262, and one related compound) and alkyl methylphosphonic acids (RMPAs, conventional nerve agent hydrolysates) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified a suitable derivatization reagent, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM), and optimized the reaction conditions. The derivatized esters of Novichok A-series degradation products were stable and easily detected. We used this derivatization to achieve the first analytical method for Novichok hydrolysis products in urine (0.40-4.0 ng/mL). The detection limits of the RMPAs (0.1-0.4 ng/mL) were comparable to those presented in previous reports involving pentafluorobenzylation or direct LC-MS/MS. The applicability of the newly developed method was evaluated by analyzing urine samples from the OPCW Fifth Biomedical Proficiency Test.

Bioanalytical Technologies for Safety Control of Fish and Seafood by Sensitive Rapid Tests for Phycotoxins
S A Eremin, A V Zherdev, M Tokeshi, A Hibara
IOP Conference Series: Earth and Environmental Science, 988, 4, 042041, 042041, IOP Publishing, 01 Feb. 2022
Scientific journal, Abstract

Wide and operate safety monitoring of foodstuffs is highly demanded in modern society. This work aims to develop and characterize new test systems for control of phycotoxins, dangerous contaminants of fish and seafood. For rapid and productive testing, 2 methods have been implemented: immunochromatographic assay and fluorescence polarization immunoassay. Various approaches for reducing the limit of detection of target analytes have been considered, including changes in the use of optical and fluorescent labels and varied order of the detected complexes formation. Antibodies and aptamers have been considered as receptor molecules. The developed techniques provide rapid (20–30 minutes, including sample preparation) and sensitive testing. Their effectiveness has been shown for different kinds of fish and seafood. Portable detectors have been proposed that allow testing directly at the sampling points, without transportation to centralized laboratories.

Microfluidic Device-Enabled Mass Production of Lipid-Based Nanoparticles for Applications in Nanomedicine and Cosmetics
Yuka Matsuura-Sawada, Masatoshi Maeki, Takaaki Nishioka, Ayuka Niwa, Jun Yamauchi, Masashi Mizoguchi, Koichi Wada, Manabu Tokeshi
ACS Applied Nano Materials, 5, 6, 7867, 7876, 2022, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Determination of blood lithium-ion concentration using digital microfluidic whole-blood separation and preloaded paper sensors
Takeshi Komatsu, Manabu Tokeshi, Shih-Kang Fan
Biosensors and Bioelectronics, 195, 113631, 113631, Elsevier BV, Jan. 2022, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Topology-Dependent Interaction of Cyclic Poly(ethylene glycol) Complexed with Gold Nanoparticles against Bovine Serum Albumin for a Colorimetric Change
Onyinyechukwu Justina Oziri, Masatoshi Maeki, Manabu Tokeshi, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Takuya Yamamoto
Langmuir, 38, 17, 5286, 5295, American Chemical Society (ACS), 08 Dec. 2021, [Peer-reviewed]
Scientific journal

Electrochemical enzyme-based blood ATP and lactate sensor for a rapid and straightforward evaluation of illness severity
Keine Nishiyama, Ryohei Mizukami, Shizuka Kuki, Akihiko Ishida, Junji Chida, Hiroshi Kido, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
Biosensors and Bioelectronics, 113832, 113832, Elsevier BV, Nov. 2021, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Microchip immunoassays for monitoring renal function: Rapid, low‐cost, and highly sensitive quantification of urinary biomarkers of diabetic nephropathy
Toshihiro Kasama, Miaomiao Sun, Noritada Kaji, Shin’Ichi Akiyama, Yukio Yuzawa, Manabu Tokeshi, Seiichi Matsuo, Yoshinobu Baba
Micromachines, 12, 11, Nov. 2021, [Peer-reviewed]
Scientific journal

Determination of Deoxynivalenol in Wheat, Barley, Corn Meal, and Wheat-Based Products by Simultaneous Multisample Fluorescence Polarization Immunoassay Using a Portable Analyzer
Ayano Nakamura, Mitsutoshi Aoyagi, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
ACS Food Science & Technology, 1, 9, 1623, 1628, American Chemical Society (ACS), 15 Oct. 2021, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Facile and rapid detection of SARS-CoV-2 antibody based on a noncompetitive fluorescence polarization immunoassay in human serum samples
Keine Nishiyama, Kazuki Takahashi, Mao Fukuyama, Motohiro Kasuya, Ayuko Imai, Takumi Usukura, Nako Maishi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Kyoko Hida, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Biosensors and Bioelectronics, 190, 113414, 113414, Elsevier BV, Oct. 2021, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Using a Paper-Based Analytical Device Designed for Remote Learning Environments to Achieve Simple Quantitative Colorimetry without Micropipettes
Takeshi Komatsu, Ryan Russel Gabatino, Harrienica Hofileña, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Journal of Chemical Education, 98, 9, 3050, 3054, 14 Sep. 2021, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Paper-Based Analytical Device for the On-Site Detection of Nerve Agents
Akinori Yamaguchi, Hajime Miyaguchi, Akihiko Ishida, Manabu Tokeshi
ACS Applied Bio Materials, 4, 8, 6512, 6518, American Chemical Society (ACS), 16 Aug. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, We report a colorimetric paper-based microfluidic device based on an enzyme inhibition assay that allows the on-site detection of nerve agents by sampling and wicking. The sample and reagents are automatically transported through the channel where an enzyme inhibition reaction is conducted, followed by an enzyme-substrate reaction and a color reaction. This device can detect 0.1 μg/mL of the nerve agent VX in a 2.5 μL drop and is nerve agent selective and robust against temperature, pH, and several liquids. We confirmed that sampling procedures (dilution and wiping) are applicable to this device. Furthermore, the fabrication procedure is easy, and the cost is at most a few tens of cents. Thus, the present device provides a practical method for the urgent detection of nerve agents in suspected chemical terrorism incidents.

Delivery of Oligonucleotides Using a Self-Degradable Lipid-Like Material.
Hiroki Tanaka, Nae Takata, Yu Sakurai, Tokuyuki Yoshida, Takao Inoue, Shinya Tamagawa, Yuta Nakai, Kota Tange, Hiroki Yoshioka, Masatoshi Maeki, Manabu Tokeshi, Hidetaka Akita
Pharmaceutics, 13, 4, 13 Apr. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, The world-first success of lipid nanoparticle (LNP)-based siRNA therapeutics (ONPATTRO®) promises to accelerate developments in siRNA therapeutics/gene therapy using LNP-type drug delivery systems (DDS). In this study, we explore the optimal composition of an LNP containing a self-degradable material (ssPalmO-Phe) for the delivery of oligonucleotides. siRNA or antisense oligonucleotides (ASO) were encapsulated in LNP with different lipid compositions. The hepatic knockdown efficiency of the target genes and liver toxicity were evaluated. The optimal compositions for the siRNA were different from those for ASO, and different from those for mRNA that were reported in a previous study. Extracellular stability, endosomal escape and cellular uptake appear to be the key processes for the successful delivery of mRNA, siRNA and ASO, respectively. Moreover, the compositions of the LNPs likely contribute to their toxicity. The lipid composition of the LNP needs to be optimized depending on the type of nucleic acids under consideration if the applications of LNPs are to be further expanded.

Simple Approach for Fluorescence Signal Amplification Utilizing a Poly(vinyl alcohol)-Based Polymer Structure in a Microchannel.
Keine Nishiyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Hideaki Hisamoto, Manabu Tokeshi
ACS Omega, 6, 12, 8340, 8345, 30 Mar. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Analytical methods with fluorescence detection are in widespread use for detecting low abundance analytes. Here, we report a simple method for fluorescence signal amplification utilizing a structure of an azide-unit pendant water-soluble photopolymer (AWP) in a microchannel. The AWP is a poly(vinyl alcohol)-based photocross-linkable polymer, which is often used in biosensors. We determined that the wall-like structure of the AWP (AWP-wall) constructed in a microchannel functioned as an amplifier of a fluorescence signal. When a solution of fluorescent molecules was introduced into the microchannel having the AWP-wall, the fluorescent molecules accumulated inside the AWP-wall by diffusion. Consequently, the fluorescence intensity inside the AWP-wall increased locally. Among the fluorescent molecules considered in this paper, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) (DDAO) showed the highest efficiency of fluorescence signal amplification. We prepared a calibration curve for DDAO using the fluorescence intensity inside the AWP-wall, and the sensitivity was 5-fold that for the microchannel without the AWP-wall. This method realizes the improved sensitivity of fluorescence detection easily because the fluorescence signal was amplified only by injecting the solution into the microchannel having the AWP-wall. Furthermore, since this method is not limited to only the use of microchannel, we expect it to be applicable in various fields.

Dip-Type Paper-Based Analytical Device for Straightforward Quantitative Detection without Precise Sample Introduction.
Takeshi Komatsu, Ryoga Maeda, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS sensors, 6, 3, 1094, 1102, 26 Mar. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, The development of low-cost, user-friendly paper-based analytical devices (PADs) that can easily measure target chemicals is attracting attention. However, most PADs require manipulation of the sample using sophisticated micropipettes for quantitative analyses, which restricts their user-friendliness. In addition, immobilization of detection molecules to cellulose fibers is essential for achieving good measuring ability as it ensures the homogeneity of color development. Here, we have described a dip-type PAD that does not require pipette manipulation for sample introduction and immobilization of detection molecules to cellulose fibers and its application to ascorbic acid (AA) and pH assays. The PAD consisted of a dipping area and two channels, each with two detection zones. The developed PADs show color distribution in the two detection zones depending on the sample flow from the dipping area. In comparison with a PAD that has one detection zone at the end of the channel, our developed device achieved higher sensitivity (limit of detection (LOD), 0.22 mg/mL) and reproducibility (maximum coefficient of variation (CV), 2.4%) in AA detection. However, in pH detection, the reproducibility of the PAD with one detection zone at the end of the channel (maximum CV, 21%) was worse than that with two zones (maximum CV, 11%). Furthermore, a dipping time over 3 s did not affect color formation or calibration curves in AA detection: LODs at 3 and 30 s dipping time were 18 and 5.8 μg/mL, respectively. The simultaneous determination of AA and pH in various beverages was performed with no significant difference compared to results of the conventional method.

Lipid nanoparticles loaded with ribonucleoprotein-oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition.
Yuichi Suzuki, Haruno Onuma, Risa Sato, Yusuke Sato, Akari Hashiba, Masatoshi Maeki, Manabu Tokeshi, Mohammad Enamul Hoque Kayesh, Michinori Kohara, Kyoko Tsukiyama-Kohara, Hideyoshi Harashima
Journal of Controlled Release, 330, 61, 71, 10 Feb. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has considerable therapeutic potential for use in treating a wide range of intractable genetic and infectious diseases including hepatitis B virus (HBV) infections. While non-viral delivery technologies for the CRISPR/Cas system are expected to have clinical applications, difficulties associated with the clinically relevant synthesis of formulations and the poor efficiency of delivery severely hinder therapeutic genome editing. We report herein on the production of a lipid nanoparticle (LNP)-based CRISPR/Cas ribonucleoprotein (RNP) delivery nanoplatform synthesized using a clinically relevant mixer-equipped microfluidic device. DNA cleavage activity and the aggregation of Cas enzymes was completely avoided under the optimized synthetic conditions. The optimized formulation, which was identified through 2 steps of design of experiments, exhibited excellent gene disruption (up to 97%) and base substitution (up to 23%) without any apparent cytotoxicity. The addition of negative charges to the RNPs by complexing single-stranded oligonucleotide (ssON) significantly enhanced the delivery of both Cas9 and Cpf1 RNPs. The optimized formulation significantly suppressed both HBV DNA and covalently closed circular DNA (cccDNA) in HBV-infected human liver cells compared to adeno-associated virus type 2 (AAV2). These findings represent a significant contribution to the development of CRISPR/Cas RNP delivery technology and its practical applications in genome editing therapy.

Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer.
Keine Nishiyama, Yohei Takeda, Kazuki Takahashi, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Haruko Ogawa, Manabu Tokeshi
Analytical and Bioanalytical Chemistry, 413, 18, 4619, 4623, 05 Feb. 2021, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Nowadays, the diagnosis of viral infections is receiving broad attention. We have developed a non-competitive fluorescence polarization immunoassay (NC-FPIA), which is a separation-free immunoassay, for a virus detection. H5 subtype avian influenza virus (H5-AIV) was used as a model virus for the proof of concept. The fluorescein-labeled Fab fragment that binds to H5 hemagglutinin was used for NC-FPIA. The purified H5-AIV which has H5 hemagglutinin was mixed with the fluorescein-labeled Fab fragment. After that, the degree of fluorescence polarization was measured with a portable FPIA analyzer. H5-AIV was successfully detected with an incubation time of 15 min. In addition, the portable FPIA analyzer enables performance of on-site NC-FPIA with a sample volume of 20 μL or less. This is the first research of detecting a virus particle by FPIA. This NC-FPIA can be applied to rapid on-site diagnosis of various viruses.

One-Step Production Using a Microfluidic Device of Highly Biocompatible Size-Controlled Noncationic Exosome-like Nanoparticles for RNA Delivery
Niko Kimura, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Applied Bio Materials, 4, 2, 1783, 1793, Feb. 2021, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Rapid, sensitive universal paper-based device enhances competitive immunoassays of small molecules.
Takeshi Komatsu, Yuki Sato, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analytica Chimica Acta, 1144, 85, 95, 01 Feb. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Competitive immunoassays comprise the standard means of detecting small molecules. However, conventional methods using microwells are difficult to apply during point-of-care tests (POCT) because they require complicated handling and are time consuming. Although paper-based analytical devices (PAD) have received considerable focus because of their rapid and straightforward operation, only a few devices have been proposed for competitive immunoassays. Herein, we describe a novel universal PAD format with a 3-dimensional configuration for competitive immunoassays that rapidly and sensitively detects small molecules. The proposed device comprised a layered structure with uniform color formation and high capture efficiency between antigen and antibody that results in rapid and reproducible results. The device rapidly (90 s) assayed biotin as a model target, with a limit of detection (LOD) of 5.08 ng mL-1, and detected progesterone with an LOD of 84 pg mL-1 within 5 min. Moreover, sample volumes and reagent consumption rates were minimized. Thus, our device could be applied to competitive immunoassays of various small molecules in POCT.

PEGylation of Silver Nanoparticles by Physisorption of Cyclic Poly(Ethylene Glycol) for Enhanced Dispersion Stability, Antimicrobial Activity, and Cytotoxicity
Onyinyechukwu Oziri, Yubo Wang, Tomohisa Watanabe, Shuya Uno, Masatoshi Maeki, Manabu Tokeshi, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Yutaka Miura, Takuya Yamamoto
Nanoscale Advances, 4, 2, 532, 545, Royal Society of Chemistry (RSC), 2021, [Peer-reviewed]
Scientific journal, Silver nanoparticles (AgNPs) are practically valuable in biological applications. However, no steady PEGylation has been established, which is essential for internal use in humans or animals. In this study, cyclic...

Three-dimensional, symmetrically assembled microfluidic device for lipid nanoparticle production
Niko Kimura, Masatoshi Maeki, Kosuke Sasaki, Yusuke Sato, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
RSC Advances, 11, 3, 1430, 1439, Jan. 2021, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Enhanced dispersion stability of gold nanoparticles by the physisorption of cyclic poly(ethylene glycol).
Yubo Wang, Jose Enrico Q Quinsaat, Tomoko Ono, Masatoshi Maeki, Manabu Tokeshi, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-Ichiro Sato, Yutaka Miura, Takuya Yamamoto
Nature communications, 11, 1, 6089, 6089, 30 Nov. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Nano-sized metal particles are attracting much interest in industrial and biomedical applications due to the recent progress and development of nanotechnology, and the surface-modifications by appropriate polymers are key techniques to stably express their characteristics. Herein, we applied cyclic poly(ethylene glycol) (c-PEG), having no chemical inhomogeneity, to provide a polymer topology-dependent stabilization for the surface-modification of gold nanoparticles (AuNPs) through physisorption. By simply mixing c-PEG, but not linear counterparts, enables AuNPs to maintain dispersibility through freezing, lyophilization, or heating. Surprisingly, c-PEG endowed AuNPs with even better dispersion stability than thiolated PEG (HS-PEG-OMe). The stronger affinity of c-PEG was confirmed by DLS, ζ-potential, and FT-IR. Furthermore, the c-PEG system exhibited prolonged blood circulation and enhanced tumor accumulation in mice. Our data suggests that c-PEG induces physisorption on AuNPs, supplying sufficient stability toward bio-medical applications, and would be an alternative approach to the gold-sulfur chemisorption.

The use of design of experiments with multiple responses to determine optimal formulations for in vivo hepatic mRNA delivery.
Akari Hashiba, Manaya Toyooka, Yusuke Sato, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima
Journal of controlled release, 327, 467, 476, 10 Nov. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Although great advances have been made in the delivery of short RNAs by lipid nanoparticles (LNPs), the optimal formulation composition and physicochemical properties of LNPs for long RNA (including mRNA) remain unclear. In the present study, we optimized the lipid composition of liver-targeted mRNA-loaded LNPs that were prepared with pH-sensitive cationic lipids that had been previously designed for siRNA delivery through a two stepped design of experiment (DoE). Multiple responses including physicochemical properties, gene expression, and liver-specificity were analyzed in order, not only to understand the role of each formulation parameter, but also to examine parameters that would be difficult to predict. We found that particle size and the PEG-to-phospholipid (PEG/PL) ratio were additional key factors for liver-specific gene expression in addition to the other formulation factors. The optimized formulation showed a better gene expression compared to other lipid formulations from industry leaders. These findings suggest that a "DoE with multiple responses" approach can be used to predict significant parameters and permit optimized formulations to be prepared more efficiently.

Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination.
Mao Fukuyama, Ayano Nakamura, Keine Nishiyama, Ayuko Imai, Manabu Tokeshi, Koji Shigemura, Akihide Hibara
Analytical Chemistry, 92, 21, 14393, 14397, 03 Nov. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH antibody). FPIA with VHH was successfully used to quantitate rabbit immunoglobulin G (IgG) and demonstrated a wider response range than that observed with antibody-binding (Fab) fragment. Then, using a portable FPIA instrument, a VHH-based immunoassay of human IgG in a human serum certified reference material was demonstrated.

Silica Nanopillar Arrays for Monitoring Diffraction-Based Label-Free Biomolecule Separation
Taiga Ajiri, Takao Yasui, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Junji Nishii, Yoshinobu Baba, Manabu Tokeshi
ACS Applied Nano Materials, 3, 9, 8810, 8816, Sep. 2020, [Last author, Corresponding author]
English, Scientific journal

Room-temperature crystallography using a microfluidic protein crystal array device and its application to protein-ligand complex structure analysis
Masatoshi Maeki, Sho Ito, Reo Takeda, Go Ueno, Akihiko Ishida, Hirofumi Tani, Masaki Yamamoto, Manabu Tokeshi
Chemical Science, 11, 34, 9072, 9087, Sep. 2020, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

One-step non-competitive fluorescence polarization immunoassay based on a Fab fragment for C-reactive protein quantification
Keine Nishiyama, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi
Sensors and Actuators B: Chemical, 128982, 128982, Elsevier BV, Sep. 2020, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer.
Keine Nishiyama, Yohei Takeda, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Yutaka Yonezawa, Kunitoshi Imai, Haruko Ogawa, Manabu Tokeshi
Sensors and Actuators. B, Chemical, 316, 128160, 128160, 01 Aug. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, A rapid, facile and selective detection of anti-H5 subtype avian influenza virus (AIV) antibody in serum by fluorescence polarization immunoassay (FPIA) was achieved. A fragment of recombinant H5 subtype AIV hemagglutinin was produced and labeled with fluorescein to use it as a labeled antigen in FPIA. This labeled antigen was mixed with anti-AIV sera (H1-H16 subtypes) and FP of the mixture was measured using a portable FP analyzer on a microdevice. It was found that FP increased in proportion to the concentration of anti-H5 AIV antibody (serum) and was significantly higher than FP obtained with the other sera. The selective detection of anti-H5 subtype AIV antibody was confirmed. The required volume of original sample was 2 μL and analysis time was within 20 min. This detection system realizes an efficient on-site diagnosis and surveillance of AIV.

Development of a Microfluidic-Based Post-Treatment Process for Size-Controlled Lipid Nanoparticles and Application to siRNA Delivery.
Niko Kimura, Masatoshi Maeki, Yusuke Sato, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
ACS Applied Materials & Interfaces, 12, 30, 34011, 34020, 29 Jul. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Microfluidic methodologies for preparation of lipid nanoparticles (LNPs) based on an organic solvent injection method enable precise size control of the LNPs. After preparation of LNPs, the organic solvent injection method needs some post-treatments, such as overnight dialysis or direct dilution with a buffer solution. LNP production using the microfluidic-based organic solvent injection method is dominated by kinetics rather than thermodynamics. Kinetics of ethanol removal from the inner and outer membranes of LNPs could induce a structural change in the membrane that could lead to fusion of LNPs. However, the effects of microfluidic post-treatment on the final size of LNPs have not been sufficiently understood. Herein, we investigated the effect of the post-treatment processes on the final product size of LNPs in detail. A simple baffle device and a model lipid system composed of a neutral phospholipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC) and cholesterol were used to produce the LNPs. We demonstrated that flow conditions of the post-treatment diluting the remaining ethanol in the LNP suspension affected the final product size of LNPs. Based on the findings, we developed an integrated baffle device composed of an LNP production region and a post-treatment region for a microfluidic-based LNP production system; this integrated baffle device prevented the undesirable aggregation or fusion of POPC LNPs even for the high-lipid-concentration condition. Finally, we applied our concept to small interfering RNA (siRNA) delivery and confirmed that no significant effects due to the continuous process occurred on the siRNA encapsulation efficiency, biological distribution, and knockdown activity. The microfluidic post-treatment method is expected to contribute to the production of LNPs for practical applications and the development of novel LNP-based nanomedicines.

Real-Time Measurement of Protein Crystal Growth Rates within the Microfluidic Device to Understand the Microspace Effect.
Masatoshi Maeki, Shohei Yamazaki, Reo Takeda, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Omega, 5, 28, 17199, 17206, 21 Jul. 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Preparation of high-quality protein crystals is a major challenge in protein crystallography. Natural convection is considered to be an uncontrollable factor of the crystallization process at the ground level as it disturbs the concentration gradient around the growing crystal, resulting in lower-quality crystals. A microfluidic environment expects an imitated microgravity environment because of the small Gr number. However, the mechanism of protein crystal growth in the microfluidic device was not elucidated due to limitations in measuring the crystal growth process within the device. Here, we demonstrate the real-time measurement of protein crystal growth rates within the microfluidic devices by laser confocal microscopy with differential interference contrast microscopy (LCM-DIM) at the nanometer scale. We confirmed the normal growth rates in the 20 and 30 μm-deep microfluidic device to be 42.2 and 536 nm/min, respectively. In addition, the growth rate of crystals in the 20 μm-deep microfluidic device was almost the same as that reported in microgravity conditions. This phenomenon may enable the development of more accessible alternatives to the microgravity environment of the International Space Station.

Observation of Ethanol-Induced Condensation and Decondensation Processes at a Single-DNA Molecular Level in Microfluidic Devices Equipped with a Rapid Solution Exchange System.
Hiroshi Suzuki, Kentaro Fujiyoshi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Chemistry, 92, 13, 9132, 9137, 07 Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Conformational transitions from secondary (e.g., B- to A-form DNA) to higher-order (e.g., coil to globule) transitions play important roles in genome expression and maintenance. Several single-molecule approaches using microfluidic devices have been used to determine the kinetics of DNA chromatin assembly because microfluidic devices can afford stretched DNA molecules through laminar flow and rapid solution exchange. However, some issues, particularly the uncertainty of time 0 in the solution exchange process, are encountered. In such kinetic experiments, it is critical to determine when the target solution front approaches the target DNA molecules. Therefore, a new design for a microfluidic device is developed that enables the instantaneous exchange of solutions in the observation channel, allowing accurate measurements of DNA conformational transitions; stepwise, ethanol-induced conformational transitions are revealed. Although full DNA contraction from coil to globule is observed with >50% ethanol, no outstanding change is observed at concentrations <40% in 10 min. With 50% ethanol solution, the DNA conformational transition passes through two steps: (i) fast and constant-velocity contraction and (ii) relatively slow contraction from the free end. The first process is attributed to the B to A conformational transition by gradual dehydration. The second process is due to the coil-globule transition as the free end of DNA starts the contraction. This globular structure formation counteracts the shear force from the microfluids and decelerates the contraction velocity. This real-time observation system can be applied to the kinetic analysis of DNA conformational transitions such as kinetics of chromatin assembly and gene expression.

Paper-Based Device for the Facile Colorimetric Determination of Lithium Ions in Human Whole Blood.
Takeshi Komatsu, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ACS Sensors, 5, 5, 1287, 1294, 22 May 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Lithium carbonate is an effective medicine for the treatment of the bipolar disorder, but the concentration of lithium in the patient's blood must be frequently monitored because of its toxicity. To date, no colorimetric methods of lithium ion detection in whole blood without pretreatment have been reported. Here, we report a colorimetric paper-based device that allows point-of-care testing in one step. This device is composed of two paper-based elements linked to each other: a blood cell separation unit and a colorimetric detection unit. After a portion of whole blood has been placed on the end of the separation unit, plasma in the sample is automatically transported to the detection unit, which displays a diagnostic color. The key feature of this device is its simple, user-friendly operation. The limit of detection is 0.054 mM and the coefficient of variance is below 6.1%, which are comparable to those of conventional instruments using the same colorimetric reaction. Furthermore, we achieved high recovery (>90%) and reproducibility (<9.8%) with spiked human blood samples. Thus, the presented device provides an alternative method for the regular monitoring of lithium concentrations in the treatment of bipolar disorder by augmenting the coefficient of variation (maximum value, 6.1%).

The Effect of Size and Charge of Lipid Nanoparticles Prepared by Microfluidic Mixing on Their Lymph Node Transitivity and Distribution.
Takashi Nakamura, Minori Kawai, Yusuke Sato, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima
Molecular Pharmaceutics, 17, 3, 944, 953, 02 Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Because the lymph node (LN) is a critical organ for inducing immune responses against pathogens and cancers, the transport of immune functional molecules such as antigens and adjuvants to LNs by delivery systems is a useful strategy for the effective outcome of an immune response. The size and charge of a delivery system largely affect the transitivity to and distribution within LN. Although pH-sensitive lipid nanoparticles (LNPs) prepared by microfluidic mixing are the latest delivery system to be applied clinically, the effects of their size and charge on the transitivity to and distribution within LN are currently unknown. We investigated the size and charge effect of LNPs prepared by microfluidic mixing on transitivity to and distribution within LNs. A 30 nm-sized LNP (30-LNP) was efficiently translocated to LNs and was taken up by CD8+ dendritic cells, while the efficiency was drastically decreased in the cases of 100 and 200 nm-sized LNPs. Furthermore, a comparative study between neutral, positively, and negatively charged 30-LNP revealed that the negative 30-LNP moved to the LN more efficiently than the other LNPs. Interestingly, the negative 30-LNP reached the deep cortex, namely, the T cell zone. Our findings provide informative insights for designing LN-targeting LNPs prepared by microfluidic mixing and for the translocation of nanoparticles in LNs.

Hydrophobic scaffolds of pH-sensitive cationic lipids contribute to miscibility with phospholipids and improve the efficiency of delivering short interfering RNA by small-sized lipid nanoparticles.
Yusuke Sato, Nana Okabe, Yusuke Note, Kazuki Hashiba, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima
Acta Biomaterialia, 102, 341, 350, 15 Jan. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Despite the fact that small-sized lipid nanoparticles (LNPs) are important for improved tissue penetration and efficient drug delivery, their poor stability and intracellular trafficking significantly hinders their use as potent small-sized LNPs. It has been reported that both the diffusion of lipid components from LNPs and the adsorption of proteins on the surface of LNPs are responsible for their decreased potency. To overcome this issue, we focused on the chemical structure of hydrophobic scaffolds of pH-sensitive cationic lipids with various lengths and shapes. LNPs composed of a pH-sensitive cationic lipid with long, linear scaffolds induced gene silencing in a dose-dependent manner, while LNPs with a classical scaffold length (C18) failed. Replacing the helper lipid from cholesterol to egg sphingomyelin (ESM) resulted in the formation of smaller LNPs with a diameter of ~22 nm and enhanced gene silencing activity. Most of the ESMs were located in the outer layer and functioned to stabilize the LNPs. Long, linear scaffolds contributed to immiscibility with phosphocholine-containing lipids including ESM. This contribution was dependent on the scaffold length of pH-sensitive cationic lipids. Although phosphocholine-containing lipids usually inhibit membrane fusion-mediated endosomal escape, long, linear scaffolds contributed to avoiding the inhibitory effect and to enhance the potency of the LNPs. These findings provide useful information needed for the rational design of pH-sensitive cationic lipid structures and the selection of appropriate helper lipids and will facilitate the development of highly potent small-sized LNPs. STATEMENT OF SIGNIFICANCE: Despite the fact that small-sized lipid nanoparticles (LNPs) are important for improved tissue penetration and efficient drug delivery, the size reduction-associated decrease in the stability and intracellular trafficking significantly hinders the development of potent small-sized LNPs. Our limited understanding of the mechanism underlying the reduced potency has also hindered the development of more potent small-sized LNPs. The findings of the present study indicate that long and linear hydrophobic scaffolds of pH-sensitive cationic lipids could overcome the loss of efficiency for nucleic acid delivery. In addition, the long hydrophobic scaffolds led to immiscibility with neutral phospholipids, resulting in efficient endosomal escape. These findings provide useful information needed for the rational design of pH-sensitive cationic lipid structures and will facilitate the development of highly potent small-sized LNPs.

Development of a Paper-based Analytical Chip for the Detection of Bacterial 16S rRNA in Wastewater Samples
Meri Nakajima, Akihiko Ishida, Manabu Tokeshi, Hisashi Satoh
BUNSEKI KAGAKU, 69, 12, 715, 722, 2020, [Peer-reviewed]
Japanese, Scientific journal

Development of an immuno-wall device for the rapid and sensitive detection of EGFR mutations in tumor tissues resected from lung cancer patients.
Naoyuki Yogo, Tetsunari Hase, Toshihiro Kasama, Keine Nishiyama, Naoya Ozawa, Takahiro Hatta, Hirofumi Shibata, Mitsuo Sato, Kazuki Komeda, Nozomi Kawabe, Kohei Matsuoka, Toyofumi Fengshi Chen-Yoshikawa, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Yoshinori Hasegawa
PloS One, 15, 11, e0241422, 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Detecting molecular targets in specimens from patients with lung cancer is essential for targeted therapy. Recently, we developed a highly sensitive, rapid-detection device (an immuno-wall device) that utilizes photoreactive polyvinyl alcohol immobilized with antibodies against a target protein via a streptavidin-biotin interaction. To evaluate its performance, we assayed epidermal growth factor receptor (EGFR) mutations, such as E746_A750 deletion in exon 19 or L858R substitution in exon 21, both of which are common in non-small cell lung cancer and important predictors of the treatment efficacy of EGFR tyrosine kinase inhibitors. The results showed that in 20-min assays, the devices detected as few as 1% (E746_A750 deletion) and 0.1% (L858R substitution) of mutant cells. Subsequent evaluation of detection of the mutations in surgically resected lung cancer specimens from patients with or without EGFR mutations and previously diagnosed using commercially available, clinically approved genotyping assays revealed diagnostic sensitivities of the immuno-wall device for E746_A750 deletion and L858R substitution of 85.7% and 87.5%, respectively, with specificities of 100% for both mutations. These results suggest that the immuno-wall device represents a good candidate next-generation diagnostic tool, especially for screening of EGFR mutations.

An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A.
Donny Nugraha Mazaafrianto, Akihiko Ishida, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
Analytical Sciences, 35, 11, 1221, 1226, 10 Nov. 2019, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
English, Scientific journal, In this study, we developed an electrochemical sensor for ochratoxin A (OTA) by using an aptamer having a dithiol-based anchor, which exhibited higher stability on a gold electrode than a monothiol-based aptamer because of its two anchors. The sensor was also based on a signal-on scheme that produces a signal current resulting from structure-switching of the aptamer upon interaction with OTA. For simple fabrication of this sensor, the non-covalent interaction of methylene blue with the aptamer was also employed as an electrochemical indicator. In this study, the performance of the sensor, including the dissociation constant of the aptamer-OTA complex, was characterized. The proposed sensor exhibited high reproducibility and enough sensitivity to detect the minimum amount of OTA required for the analysis of real food samples with a limit of detection of 113 pM.

A paper-based analytical device coupled with electrochemical detection for the determination of dexamethasone and prednisolone in adulterated traditional medicines.
Vitsarut Primpray, Orawon Chailapakul, Manabu Tokeshi, Theerasak Rojanarata, Wanida Laiwattanapaisal
Analytica Chimica Acta, 1078, 16, 23, 31 Oct. 2019, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, The adulteration of herbal medicines by dexamethasone or prednisolone is regarded as a serious problem in many communities. Herein, a novel platform for the separation and quantification of both target steroids in herbal medicines based on electrochemical paper-based analytical devices (ePADs) has been created. The ePAD was composed of Whatman SG81 chromatography paper, 3D-printed devices and a commercial screen-printed electrode. Whatman SG81 silica-coated paper was used for the separation of dexamethasone and prednisolone based on the difference in their partition coefficients during the flow of the mobile phase. The optimal mobile phase was composed of 60% ethyl acetate in cyclohexane and required 7 min for separation. The separated steroids on the paper were then quantified by electrochemical detection using differential pulse voltammetry, in which the 3D-printed devices facilitated the measurement. Analytical detection ranges of 10-500 μg mL-1 were obtained for both dexamethasone and prednisolone (r2 = 0.988 and 0.994, respectively). The limits of detection for dexamethasone and prednisolone were 3.59 and 11.98 μg mL-1, respectively, whereas the limits of quantification were 6.00 and 20.02 μg mL-1, respectively. The amounts of both target steroids derived from real herbal medicine samples determined by the proposed method were comparable to those obtained with assays using standard high-performance liquid chromatography. In addition, a simple evaporation step can be used to increase the concentration of the samples before analysis. These ePADs are simple, low-cost, rapid, and very promising for on-site quantitative detection.

Rapid, Sensitive, and Selective Detection of H5 Hemagglutinin from Avian Influenza Virus Using an Immunowall Device.
Kenia Chávez Ramos, Keine Nishiyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Toshihiro Kasama, Yoshinobu Baba, Manabu Tokeshi
ACS Omega, 4, 15, 16683, 16688, 08 Oct. 2019, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Avian influenza virus (AIV) infection, caused by influenza virus type A, is an infectious, acute respiratory disease of birds related to influenza outbreaks worldwide. The highly pathogenic AIV subtype H5N1 has crossed species barriers to infect mammals, including humans, with fatal outcomes and has received attention as a potential pandemic threat. A rapid and timely detection in poultry is vitally important to prevent the virus spread. Despite their great sensitivity, conventional detection methods such as real-time reverse transcription-polymerase chain reaction and the agar gel precipitation test are time-consuming and labor-intensive and require special training. In this work, an immunowall device was evaluated as an easier and faster way for detecting AIV H5-hemagglutinin (AIV H5-HA). For detection, fluorescence-labeled or enzyme-labeled antibody was employed as a labeling antibody in a sandwich immunoassay. Both were shown in this paper to be easier and faster assays for detection compared with the conventional enzyme-linked immunosorbent assay (ELISA) kit. In addition, high selectivity was achieved for AIV H5-HA detection after the evaluation of other different HA virus subtypes. The limit of detection was 0.23 ng/mL for the enzyme-labeled antibody. This value was equivalent to that of the conventional ELISA kit but 8 times faster (31 min compared to 260 min). The detection range was 0.23-100 ng/mL. The immunowall device with the enzyme-labeled antibody offers a rapid, sensitive, selective, and simple immunoassay system for future H5 AIV real sample detection.

High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer.
Osamu Wakao, Ken Satou, Ayano Nakamura, Polina A Galkina, Keine Nishiyama, Ken Sumiyoshi, Fumio Kurosawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Mikhail A Proskurnin, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Lab on a Chip, 19, 15, 2581, 2588, 07 Aug. 2019, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, High-throughput fluorescence polarization immunoassays (FPIAs) for mycotoxin were conducted using a portable FP analyzer with a microdevice. Simultaneous FPIA measurements for 8 different deoxynivalenol (DON) concentrations in 12 chambers (total of 96 samples) and high-throughput FPIA measurements for single DON concentrations in more than 500 chambers were conducted. The results indicated that simultaneous FPIAs for 96 independent samples and for 500 samples were possible by FP imaging. The FP analyzer has a size of 65 cm (W 35 cm × D 15 cm × H 15 cm) and costs less than $5000. The sample volume was 1 nL. Furthermore, it is expected that sample reaction and FP detection can be automatically conducted with the analyzer by changing the microdevice and the software. Its features such as low cost and portability will contribute to on-site measurement and point-of-care testing. Additionally, the high-throughput feature will contribute to the study of molecular interactions based on FP measurements.

Ultrasensitive detection of disease biomarkers using an immuno-wall device with enzymatic amplification.
Keine Nishiyama, Toshihiro Kasama, Seiya Nakamata, Koya Ishikawa, Daisuke Onoshima, Hiroshi Yukawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi
The Analyst, 144, 15, 4589, 4595, 07 Aug. 2019, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, We present an ultrasensitive immunoassay system for disease biomarkers utilizing the immuno-wall device and an enzymatic amplification reaction. The immuno-wall device consisted of 40 microchannels, each of which contained an antibody-modified wall-like structure along the longitudinal axis of the microchannel. The wall was fabricated with a water-soluble photopolymer containing streptavidin by photolithography, and biotinylated capture antibodies were immobilized on the sides through streptavidin-biotin interaction. For an assay, introducing the target biomarker and secondary and labeled antibodies produced a sandwich complex anchored on the sides of the wall. A conventional immuno-wall device uses a fluorescence-labeled antibody as a labeling antibody. To achieve an ultrasensitive detection of a trace biomarker, we used an enzyme label and amplified the signal with the enzymatic reaction with a fluorogenic substrate in the microchannel. The highest signal/background ratio was obtained by using alkaline phosphatase-labeled antibody and 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate. To evaluate the device performance, we detected human C-reactive protein (CRP) as a model biomarker. The detection limit (LOD) of CRP in phosphate-buffered saline was 2.5 pg mL-1 with a sample volume of 0.25 μL. This LOD was approximately 3 orders of magnitude lower than that obtained with fluorescent-dye (DyLight 650)-labeled antibody. In addition, the present device provided a wide detection range of 0.0025-10 ng mL-1 for CRP. We successfully developed an ultrasensitive immunoassay system with simple operation and only a small sample volume.

The Use of a Microfluidic Device to Encapsulate a Poorly Water-Soluble Drug CoQ10 in Lipid Nanoparticles and an Attempt to Regulate Intracellular Trafficking to Reach Mitochondria.
Mitsue Hibino, Yuma Yamada, Naoki Fujishita, Yusuke Sato, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima
Journal of Pharmaceutical Sciences, 108, 8, 2668, 2676, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, A number of drugs that are currently on the market, as well as new candidates for drugs, are poorly water soluble. Because of this, a need exists to develop drug formulations that will permit the expanded use of such drugs. The use of liposomes and lipid nanoparticles for drug delivery has attracted attention as a technique for solubilizing molecules that are poorly water soluble, but this technique faces serious scale-up risks. In this study, we report on attempts to encapsulate Coenzyme Q10 (CoQ10) as a model of a poorly water-soluble drug in an MITO-Porter, a liposome for mitochondrial delivery using a microfluidic device (a CoQ10-MITO-Porter [μ]). The physical properties of the CoQ10-MITO-Porter [μ] including homogeneity, size, and preparation volume were compared with those for a CoQ10-MITO-Porter prepared by the ethanol dilution method (a CoQ10-MITO-Porter [ED]). In the case where a microfluidic device was used, a small-sized CoQ10-MITO-Porter was formed homogeneously, and it was possible to prepare it on a large scale. Intracellular observations using HeLa cells showed that the CoQ10-MITO-Porter [μ] was efficiently internalized by cells to reach mitochondria. These results indicate that the CoQ10-MITO-Porter [μ] represents a potential candidate for use in mitochondrial nanomedicine.

A Concentric Ring Array Electrode for a Wall-Jet Cell in a Microfluidic Device　　　　　　　　　　　　　　　
Keine Nishiyama, Koki Hoshikawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Electroanalysis, 31, 1736, 1743, Jun. 2019, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Sensitive Fluorescent Polarization Immunoassay by Optimizing Synchronization Mismatch Condition
Osamu Wakao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi
Sensors and Actuators B, 285, 418, 422, Apr. 2019, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Understanding structure-activity relationships of pH-sensitive cationic lipids facilitates the rational identification of promising lipid nanoparticles for delivering siRNAs in vivo
Yusuke Sato, Kazuki Hashiba, Kosuke Sasaki, Masatoshi Maeki, Manabu Tokeshi, Hideyoshi Harashima
Journal of Controlled Release, 295, 140, 152, Elsevier BV, Feb. 2019, [Peer-reviewed]
English, Scientific journal

Development of a Microdevice for Facile Analysis of Theophylline in Whole Blood by a Cloned Enzyme Donor Immunoassay　　　　　　　　　　　　　　　
Keine Nishiyama, Kanako Sugimura, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Lab on a Chip, 19, 233, 240, Feb. 2019, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Label-Free Electrochemical Sensor for Ochratoxin A Uisng a Microfabricated Electrode with Immobilized Aptamer
Donny N. Mazaafrianto, Akihiko Ishida, Masatoshi Maeki, Hirofumi Tani, Manabu Tokeshi
ACS Omega, 3, 12, 16823, 16830, American Chemical Society (ACS), Dec. 2018, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Development of the iLiNP Device: Fine Tuning the Lipid Nanoparticle Size within 10 nm for Drug Delivery
Niko Kimura, Masatoshi Maeki, Yusuke Sato, Yusuke Note, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
ACS Omega, 3, 5, 5044, 5051, American Chemical Society, 09 May 2018, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Recent Microdevice-based Aptamer Sensors
Manabu Tokeshi
Micromachines, 9, 5, 1, 25, MDPI AG, Apr. 2018, [Peer-reviewed], [Invited], [Last author, Corresponding author]
English, Scientific journal, Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques, such as electrochemical and optical detection, have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.

Microfluidic Devices for Drug Delivery Systems
Manabu Tokeshi
Advanced Drug Delivery Reviews, 128, 1, 2, Elsevier B.V., 15 Mar. 2018, [Peer-reviewed], [Invited], [Corresponding author]
English, Scientific journal

Advances in microfluidics for lipid nanoparticles and extracellular vesicles and applications in drug delivery systems
Masatoshi Maeki, Niko Kimura, Yusuke Sato, Hideyoshi Harashima, Manabu Tokeshi
Advanced Drug Delivery Reviews, 128, 84, 100, Elsevier B.V., 15 Mar. 2018, [Peer-reviewed], [Invited], [Last author, Corresponding author]
English, Scientific journal

A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer
Osamu Wakao, Ken Satou, Ayano Nakamura, Ken Sumiyoshi, Masanori Shirokawa, Chikaaki Mizokuchi, Kunihiro Shiota, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Review of Scientific Instruments, 89, 2, 024103, American Institute of Physics Inc., 01 Feb. 2018, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Characteristics of microfluidic paper-based analytical devices fabricated by four different methods
Takeshi Komatsu, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analytical Sciences, 34, 1, 39, 44, Japan Society for Analytical Chemistry, 2018, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Dynamic wettability of polyethylene glycol-modified poly(dimethylsiloxane) surfaces in an aqueous/organic two-phase system
Mao Fukuyama, Manabu Tokeshi, Mikhail A. Proskurnin, Akihide Hibara
Lab on a Chip, 18, 2, 356, 361, Royal Society of Chemistry, 2018, [Peer-reviewed], ［Internationally co-authored］
English, Scientific journal

マイクロデバイスを用いたタンパク質の立体構造解析
真栄城 正寿, 宮崎 真佐也, 渡慶次 学
ぶんせき, 516, 575, 580, (公社)日本分析化学会, Dec. 2017
Japanese

Understanding the formation mechanism of lipid nanoparticles in microfluidic devices with chaotic micromixers
Masatoshi Maeki, Yuka Fujishima, Yusuke Sato, Takao Yasui, Noritada Kaji, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Hideyoshi Harashima, Manabu Tokeshi
PLoS ONE, 12, 11, e0187962, Nov. 2017, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Using Laser Interference Lithography in the Fabrication of a Simplified Micro and Nanofluidic Device for Label-free Detection
Taiga Ajiri, Haruya Kasa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Junji Nishi, Manabu Tokeshi
Analytical Sciences, 33, 10, 1197, 1199, Oct. 2017, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Optimization of the nanofluidic design for label-free detection of biomolecules using a nanowall array
Taiga Ajiri, Takao Yasui, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi
Sensors and Actuators B-Chemical, 250, 39, 43, Oct. 2017, [Peer-reviewed], [Corresponding author]
English, Scientific journal

A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits
Qiong Wu, Noritada Kaji, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Manabu Tokeshi, Tomoji Kawai, Yoshinobu Baba
Scientific Reports, 7, 43877, Mar. 2017, [Peer-reviewed]
English, Scientific journal

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips
Saeed Mohammadi, Lori Shayne Alamo Busa, Masatoshi Maeki, Reza M. Mohamadi, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analytical Sciences, 32, 12, 1359, 1362, Dec. 2016, [Peer-reviewed]
English, Scientific journal

Novel concept of washing for microfluidic paper-based analytical devices based on capillary force of paper substrates
Saeed Mohammadi, Lori Shayne Alamo Busa, Masatoshi Maeki, Reza M. Mohamadi, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analytical and Bioanalytical Chemistry, 408, 27, 7559, 7563, Nov. 2016, [Peer-reviewed]
English, Scientific journal

Simple and sensitive colorimetric assay system for horseradish peroxidase using microfluidic paper-based devices
Lori Shayne Alamo Busa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Sensors and Actuators B: Chemical, 236, 433, 441, Nov. 2016
English, Scientific journal

Label-free detection of real-time DNA amplification using a nanofluidic diffraction grating
Takao Yasui, Kensuke Ogawa, Noritada Kaji, Mats Nilsson, Taiga Ajiri, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba
Scientific Reports, 6, 31642, Aug. 2016, [Peer-reviewed]
English, Scientific journal

3,3 ',5,5 '-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays
Lori Shayne Alamo Busa, Takeshi Komatsu, Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
ANALYTICAL SCIENCES, 32, 8, 815, 818, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Microfluidic Autologous Serum Eye-Drops Preparation as a Potential Dry Eye Treatment
Takao Yasui, Jumpei Morikawa, Noritada Kaji, Manabu Tokeshi, Kazuo Tsubota, Yoshinobu Baba
Micromachines, 7, 7, 113, Jul. 2016, [Peer-reviewed]
English, Scientific journal

Elucidation of the physicochemical properties and potency of siRNA-loaded small-sized lipid nanoparticles for siRNA delivery
Yusuke Sato, Yusuke Note, Masatoshi Maeki, Noritada Kaji, Yoshinobu Baba, Manabu Tokeshi, Hideyoshi Harashima
Journal of Controlled Release, 229, 48, 57, May 2016, [Peer-reviewed]
English, Scientific journal

An Easy-to-Use Polystyrene Microchip-based Cell Culture System
Hidekatsu Tazawa, Shohei Sunaoshi, Manabu Tokeshi, Takehiko Kitamori, Ritsuko Ohtani-Kaneko
Analytical Sciences, 32, 3, 349, 353, Mar. 2016, [Peer-reviewed]
English, Scientific journal

Microfluidic Autologous Serum Eye-Drops Preparation as a Potential Dry Eye Treatment.
Takao Yasui, Jumpei Morikawa, Noritada Kaji, Manabu Tokeshi, Kazuo Tsubota, Yoshinobu Baba
Micromachines, 7, 7, 113, 113, 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal

Advances in microfluidic paper-based analytical devices for food and water analysis
Lori Shayne Alamo Busa, Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Micromachines, 7, 5, 1, 21, MDPI AG, 2016, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Microfluidic approaches for protein crystal structure analysis
Masatoshi Maeki, Hiroshi Yamaguchi, Manabu Tokeshi, Masaya Miyazaki
Analytical Sciences, 32, 1, 3, 9, Japan Society for Analytical Chemistry, 2016, [Peer-reviewed]
English, Scientific journal

A competitive immunoassay system for microfluidic paper-based analytical detection of small size molecules
Lori Shayne Alamo Busa, Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analyst, 141, 24, 6598, 6603, 2016, [Peer-reviewed]
English, Scientific journal

Image analysis for a microfluidic paper-based analytical device using the CIE L(star)a(star)b(star) color system
Takeshi Komatsu, Saeed Mohammadi, Lori Shayne Alamo Busa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analyst, 141, 24, 6507, 6509, 2016, [Peer-reviewed]
English, Scientific journal

A microfluidic-based protein crystallization method in 10 micrometer-sized crystallization space
Masatoshi Maeki, Shohei Yamazaki, Ashtamurthy S. Pawate, Akihiko Ishida, Hirofumi Tani, Kenichi Yamashita, Masakazu Sugishima, Keiichi Watanabe, Manabu Tokeshi, Paul J. A. Kenis, Masaya Miyazaki
CrystEngComm, 18, 40, 7722, 7727, 2016, [Peer-reviewed]
English, Scientific journal

An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas
Akane Yamamichi, Toshihiro Kasama, Fumiharu Ohka, Hiromichi Suzuki, Akira Kato, Kazuya Motomura, Masaki Hirano, Melissa Ranjit, Lushun Chalise, Michihiro Kurimoto, Goro Kondo, Kosuke Aoki, Noritada Kaji, Manabu Tokeshi, Toshio Matsubara, Takeshi Senga, Mika K. Kaneko, Hidenori Suzuki, Masahito Hara, Toshihiko Wakabayashi, Yoshinobu Baba, Yukinari Kato, Atsushi Natsume
Science and Technology of Advanced Materials, 17, 1, 618, 625, 2016, [Peer-reviewed]
English, Scientific journal

A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed Column
Akihiko Ishida, Mitsutaka Fujii, Takehiro Fujimoto, Shunsuke Sasaki, Ichiro Yanagisawa, Hirofumi Tani, Manabu Tokeshi
Analytical Sciences, 31, 11, 1163, 1169, 10 Nov. 2015, [Peer-reviewed]
English, Scientific journal

Micropillars Fabricated on Poly(methyl methacrylate) Substrates for Separation of Microscale Objects
Takao Yasui, Satoru Ito, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Sciences, 31, 11, 1197, 1200, Nov. 2015, [Peer-reviewed]
English, Scientific journal

Fluorescence Polarization Measurement System Using a Liquid Crystal Layer and an Image Sensor
Osamu Wakao, Yusaku Fujii, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi
Analytical Chemistry, 87, 19, 9647, 9652, Oct. 2015, [Peer-reviewed]
English, Scientific journal

A microfluidic cell culture system for monitoring of sequential changes in endothelial cells after heat stress
Hidekatsu Tazawa, Kenjiro Sato, Atsuhiro Tsutiya, Manabu Tokeshi, Ritsuko Ohtani-Kaneko
Thrombosis Research, 136, 2, 328, 334, Aug. 2015, [Peer-reviewed]
English, Scientific journal

Arrangement of a Nanostructure Array To Control Equilibrium and Nonequilibrium Transports of Macromolecules
Takao Yasui, Noritada Kaji, Ryo Ogawa, Shingi Hashioka, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba
Nano Letters, 15, 5, 3445, 3451, May 2015, [Peer-reviewed]
English, Scientific journal

A Method of Cryoprotection for Protein Crystallography by Using a Microfluidic Chip and Its Application for in Situ X-ray Diffraction Measurements
Masatoshi Maeki, Ashtamurthy S. Pawate, Kenichi Yamashita, Masahide Kawamoto, Manabu Tokeshi, Paul J. A. Kenis, Masaya Miyazaki
Analytical Chemistry, 87, 8, 4194, 4200, Apr. 2015, [Peer-reviewed]
English, Scientific journal

An instrument-free, screen-printed paper microfluidic device that enables bio and chemical sensing
Saeed Mohammadi, Masatoshi Maeki, Reza M. Mohamadi, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Analyst, 140, 19, 6493, 6499, 2015, [Peer-reviewed]
English, Scientific journal

Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture
Toyohito Naito, Noritada Kaji, Manabu Tokeshi, Takuya Kubo, Yoshinobu Baba, Koji Otsuka
Analytical Methods, 7, 17, 7264, 7269, 2015, [Peer-reviewed]
English, Scientific journal

Rapid, highly sensitive, and simultaneous detection of staphylococcal enterotoxins in milk by using immuno-pillar devices
Toshihiro Kasama, Mai Ikami, Wanchun Jin, Keiko Yamada, Noritada Kaji, Yusuke Atsumi, Makoto Mizutani, Atsushi Murai, Akira Okamoto, Takao Namikawa, Michio Ohta, Manabu Tokeshi, Yoshinobu Baba
Analytical Methods, 7, 12, 5092, 5095, 2015, [Peer-reviewed]
English, Scientific journal

A strategy for synthesis of lipid nanoparticles using microfluidic devices with a mixer structure
Masatoshi Maeki, Tatsuyoshi Saito, Yusuke Sato, Takao Yasui, Noritada Kaji, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Hideyoshi Harashima, Manabu Tokeshi
RSC Advances, 5, 57, 46181, 46185, 2015, [Peer-reviewed]
English, Scientific journal

Development of High-performance Immuno-pillar Devices: Improvement of Antibody-immobilized Solid Support
NISHIWAKI Nanako, KASAMA Toshihiro, ISHIDA Akihiko, TANI Hirofumi, BABA Yoshinobu, TOKESHI Manabu
BUNSEKI KAGAKU, 64, 5, 329, 335, Japan Society for Analytical Chemistry, 2015, [Peer-reviewed]
Japanese, Scientific journal, In order to realize ultra-early diagnosis of disease in practical applications, we have fabricated next-generation immuno-pillar devices with higher sensitivity. In the newly developed devices, capture antibodies were immobilized on affinity beads based on chemical bonding, while in the previous-generation ones, polystyrene beads were used for physical adsorption-based immobilization. To evaluate the sensitivity of the next-generation immuno-pillar device, we quantitatively analyzed C-reactive protein (CRP). The limit of detection was estimated to be 0.1 ng mL−1 (total assay time, 23 min), which was twoorders of magnitude lower than that obtained by using the previous-generation immuno-pillar device, and was low enough to perform the CRP test. In addition, we investigated the storage stability of the immuno-pillar device, and confirmed that the device can retain its performance for over 9 months.

Development of a Micro RNA Extraction Chip from Human Tumor Cells
OKAMOTO Yukihiro, HIBINO Ayato, KAJI Noritada, TOKESHI Manabu, BABA Yoshinobu
BUNSEKI KAGAKU, 64, 1, 9, 13, The Japan Society for Analytical Chemistry, 2015, [Peer-reviewed]
Japanese, Scientific journal, The importance of micro RNA analysis has been significantly increasing because the role of micro RNA in the human body has been gradually revealed. Despite its importance, the analysis has suffered from several troublesome pretreatments, which hamper any easy analysis. Therefore, an easy and high-throughput pretreatment method has been demanded. In this paper, we focused on the great advantages of microchip pretreatments over conventional manual pretreatments and developed a microchip for micro RNA extraction. To simplify microchip fabrication, we adopted poly(dimethyl siloxane) (PDMS) microchip and a silica membrane, which has rolls in RNA extraction fields. With silica membranes and the adhesion nature of PDMS, we could easily fabricate RNA extraction fields in the microchip. With this microchip, we successfully extracted micro RNA from cancer tumor cells. Though this is a preliminary experiment, and still has many improvement points, our device is expected to be applied for easy and fast micro RNA extraction from biological samples.

Synthesis and Reactions of Ynolates via a Stop-Flow Method with a Flow Microreactor
Toshiya Yoshiiwa, Satoshi Umezu, Manabu Tokeshi, Yoshinobu Baba, Mitsuru Shindo
Journal of Flow Chemistry, 4, 4, 180, 184, Dec. 2014, [Peer-reviewed]
English, Scientific journal

Bioluminescence immunoassay using luciferase-encapsulated liposome as a label
Yusuke Nakatani, Chiaki Shido, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Luminescence, 29, 83, 84, Aug. 2014, [Peer-reviewed]
English

Bioluminescence Assay Based on Controlled Inhibitory Effect on Firefly Luciferase
Ryoko Kurishiba, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Luminescence, 29, 78, 78, Aug. 2014, [Peer-reviewed]
English

Effect of reductants on oscillatory chemiluminescence of fluorescein catalyzed by horseradish peroxidase
Hirofumi Tani, Ai Masuyama, Akihiko Ishida, Manabu Tokeshi
Luminescence, 29, 99, 100, Aug. 2014, [Peer-reviewed]
English

Generation of ynolates via reductive lithiation using flow microreactors
Satoshi Umezu, Toshiya Yoshiiwa, Manabu Tokeshi, Mitsuru Shindo
Tetrahedron Letters, 55, 10, 1822, 1825, Mar. 2014, [Peer-reviewed]
English, Scientific journal

Microfluidic biosensor for the detection of DNA by fluorescence enhancement and the following streptavidin detection by fluorescence quenching
Jun Wang, Michihiko Aki, Daisuke Onoshima, Kenji Arinaga, Noritada Kaji, Manabu Tokeshi, Shozo Fujita, Naoki Yokoyama, Yoshinobu Baba
Biosensors and Bioelectronics, 51, 280, 285, Jan. 2014, [Peer-reviewed]
English, Scientific journal

Nanopillar array chip integrated with on-line stacking for fast DNA separation with high sensitivity and high resolution
Takao Yasui, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba
Microfluidics and Nanofluidics, 14, 6, 961, 967, Jun. 2013, [Peer-reviewed]
English, Scientific journal

Highly efficient electrochemical valence control of uranium using microfluidic chip equipped with microelectrodes
Takehiko Tsukahara, Hiroyasu Hotokezaka, Masayuki Harada, Yoshikuni Kikutani, Manabu Tokeshi, Yasuhisa Ikeda
Microfluidics and Nanofluidics, 14, 6, 989, 994, Jun. 2013, [Peer-reviewed]
English, Scientific journal

DNA manipulation and separation in sublithographic-scale nanowire array
Takao Yasui, Sakon Rahong, Koki Motoyama, Takeshi Yanagida, Qiong Wu, Noritada Kaji, Masaki Kanai, Kentaro Doi, Kazuki Nagashima, Manabu Tokeshi, Masateru Taniguchi, Satoyuki Kawano, Tomoji Kawai, Yoshinobu Baba
ACS Nano, 7, 4, 3029, 3035, Apr. 2013, [Peer-reviewed]
English, Scientific journal

Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method
Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Takao Yasui, Manabu Tokeshi, Yoshinobu Baba
Analytical Sciences, 29, 3, 367, 371, Mar. 2013, [Peer-reviewed]
English, Scientific journal

Application of IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products.
Wanchun Jin, Keiko Yamada, Mai Ikami, Noritada Kaji, Manabu Tokeshi, Yusuke Atsumi, Makoto Mizutani, Atsushi Murai, Akira Okamoto, Takao Namikawa, Yoshinobu Baba, Michio Ohta
Journal of Microbiological Methods, 92, 3, 323, 31, Mar. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal, Staphylococcal enterotoxins (SEs), produced by Staphylococcus aureus, are a major cause of staphylococcal food poisoning. Traditionally, sandwich enzyme-linked immunosorbent assay (ELISA) and reverse passive latex agglutination with rabbit antibody IgG have been used to detect SEs. However, most of these kits require a long processing time and there is a risk of false-positive results since IgG reacts nonspecifically with protein A produced by S. aureus. In this study, we prepared antienterotoxin chicken IgY antibodies specific for each SE (SEA to SEE) without reaction to protein A, which enabled a drastic reduction in nonspecific reactions. ELISAs, lateral flow device (LFDs), and IgY-based immunopillar chips were developed for SE detection. All the ELISAs developed were as sensitive as commercially available kits. The SEs in milk were successfully detected by the ELISAs, LFDs, and immunopillar chips without any sample pretreatment. The LFD could detect SEA even at the low concentration of 0.2 ng/ml within 15 min in milk. The detection limit of the immunopillar chips for the SEs ranged from 0.01 to 0.1 ng/ml in milk; the SEs were detected within 12 min and specialized skills were not required. The ELISA and LFD detected SEA in dairy products artificially contaminated with S. aureus, including ice cream, yogurt, and café au lait, in a dose-dependent manner. In conclusion, IgY allows highly specific detection of SEs, and ELISAs, LFDs, and immunopillar chips should be useful tools for screening SEs in milk and dairy products.

Quantum Dots Conjugated with Transferrin for Brain Tumor Cell Imaging　　　　　　　　　　　　　　　
Hiroshi Yukawa, Ryoko Tsukamoto, Ayano Kano, Yukihiro Okamoto, Manabu Tokeshi, Tetsuya Ishikawa, Masaaki Mizuno, Yoshinobu Baba
Journal of Cell Science & Therapy, 4, 150-1, 150-7, 2013, [Peer-reviewed]
English, Scientific journal

Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules
Takao Yasui, Koki Motoyama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
RSC Advances, 3, 10, 3237, 3240, 2013, [Peer-reviewed]
English, Scientific journal

Temperature-driven self-actuated microchamber sealing system for highly integrated microfluidic devices
Toyohiro Naito, Rerngchai Arayanarakool, Severine Le Gac, Takao Yasui, Noritada Kaji, Manabu Tokeshi, Albert van den Berg, Yoshinobu Baba
Lab on a Chip, 13, 3, 452, 458, 2013, [Peer-reviewed]
English, Scientific journal

Inkjet Injection of DNA Droplets for Microchannel Array Electrophoresis
Takao Yasui, Yosuke Inoue, Toyohiro Naito, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Chemistry, 84, 21, 9282, 9286, Nov. 2012, [Peer-reviewed]
English, Scientific journal

Fabrication of Functionalized Double-Lamellar Multifunctional Envelope-Type Nanodevices Using a Microfluidic Chip with a Chaotic Mixer Array
Katsuma Kitazoe, Yeon-Su Park, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Kentaro Kogure, Hideyoshi Harashima, Yoshinobu Baba
PLOS ONE, 7, 6, e39057-1, 8, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Monitoring transplanted adipose tissue-derived stem cells combined with heparin in the liver by fluorescence imaging using quantum dots
Hiroshi Yukawa, Masaki Watanabe, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshitaka Miyamoto, Hirofumi Noguchi, Yoshinobu Baba, Shuji Hayashi
Biomaterials, 33, 7, 2177, 2186, Mar. 2012, [Peer-reviewed]
English, Scientific journal

Introducing carbon nanotubes into living walled plant cells through cellulase-induced nanoholes
Maged F. Serag, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
RSC Advances, 2, 2, 398, 400, 2012, [Peer-reviewed]
English, Scientific journal

Confocal Microscopic Evaluation of Mixing Performance for Three-Dimensional Microfluidic Mixer
Takao Yasui, Yusuke Omoto, Keiko Osato, Noritada Kaji, Norikazu Suzuki, Toyohiro Naito, Yukihiro Okamoto, Manabu Tokeshi, Eiji Shamoto, Yoshinobu Baba
Analytical Sciences, 28, 1, 57, 59, Jan. 2012, [Peer-reviewed]
English, Scientific journal

The plant cell uses carbon nanotubes to build tracheary elements
Maged F. Serag, Noritada Kaji, Manabu Tokeshi, Alberto Bianco, Yoshinobu Baba
Integrative Biology, 4, 2, 127, 131, 2012, [Peer-reviewed]
English, Scientific journal

Aqueous Phase Synthesized CdSe Magic-Sized Clusters: Solution Composition Dependence of Adsorption Layer Structure
Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Journal of Nanoscience and Nanotechnology, 12, 1, 539, 546, Jan. 2012, [Peer-reviewed]
English, Scientific journal

A deep microfluidic absorbance detection cell replicated from a thickly stacked SU-8 dry film resist mold
Daisuke Onoshima, Jun Wang, Michihiko Aki, Kenji Arinaga, Noritada Kaji, Manabu Tokeshi, Shozo Fujita, Naoki Yokoyama, Yoshinobu Baba
Analytical Methods, 4, 12, 4368, 4372, 2012, [Peer-reviewed]
English, Scientific journal

Characterization of low viscosity polymer solutions for microchip electrophoresis of non-denatured proteins on plastic chips
Takao Yasui, Mohamad Reza Mohamadi, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
Biomicrofluidics, 5, 4, 044114-1, 9, Dec. 2011, [Peer-reviewed]
English, Scientific journal

Estimation of the Distribution of Intravenously Injected Adipose Tissue-Derived Stem Cells Labeled with Quantum Dots in Mice Organs through the Determination of their Metallic Components by ICPMS
Yuka Takasaki, Masaki Watanabe, Hiroshi Yukawa, Akhmad Sabarudin, Kazumi Inagaki, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshitaka Miyamoto, Hirofumi Noguchi, Tomonari Umemura, Shuji Hayashi, Yoshinobu Baba, Hiroki Haraguchi
Analytical Chemistry, 83, 21, 8252, 8258, Nov. 2011, [Peer-reviewed]
English, Scientific journal

Rapid qualitative evaluation of DNA transcription factor NF-kappa B by microchip electrophoretic mobility shift assay in mammalian cells
Sonoko Inoue, Noritada Kaji, Masatoshi Kataoka, Yasuo Shinohara, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
ELECTROPHORESIS, 32, 22, 3241, 3247, Nov. 2011, [Peer-reviewed]
English, Scientific journal

Functional Platform for Controlled Subcellular Distribution of Carbon Nanotubes
Maged F. Serag, Noritada Kaji, Enrica Venturelli, Yukihiro Okamoto, Kazuyoshi Terasaka, Manabu Tokeshi, Hajime Mizukami, Kevin Braeckmans, Alberto Bianco, Yoshinobu Baba
ACS Nano, 5, 11, 9264, 9270, Nov. 2011, [Peer-reviewed]
English, Scientific journal

Aqueous phase-synthesized small CdSe quantum dots: adsorption layer structure and strong band-edge and surface trap emission
Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Journal of Nanoparticle Research, 13, 11, 5781, 5798, Nov. 2011, [Peer-reviewed]
English, Scientific journal

Extraction of Am(III) at the Interface of Organic-Aqueous Two-Layer Flow in a Microchannel
Yasutoshi Ban, Yoshikuni Kikutani, Manabu Tokeshi, Yasuji Morita
Journal of Nuclear Science and Technology, 48, 10, 1313, 1318, Oct. 2011, [Peer-reviewed]
English, Scientific journal

A clinical trial for therapeutic drug monitoring using microchip-based fluorescence polarization immunoassay
Tomoya Tachi, Tetsunari Hase, Yukihiro Okamoto, Noritada Kaji, Takeshi Arima, Hiroyuki Matsumoto, Masashi Kondo, Manabu Tokeshi, Yoshinori Hasegawa, Yoshinobu Baba
Analytical and Bioanalytical Chemistry, 401, 7, 2301, 2305, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Electroosmotic Flow in Microchannels with Nanostructures
Takao Yasui, Noritada Kaji, Mohamad Reza Mohamadi, Yukihiro Okamoto, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba
ACS Nano, 5, 10, 7775, 7780, Oct. 2011, [Peer-reviewed]
English, Scientific journal

DNA Separation in Nanowall Array Chips
Takao Yasui, Noritada Kaji, Ryo Ogawa, Shingi Hashioka, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba
Analytical Chemistry, 83, 17, 6635, 6640, Sep. 2011, [Peer-reviewed]
English, Scientific journal

Label-Free Detection of DNA-Binding Proteins Based on Microfluidic Solid-State Molecular Beacon Sensor
Jun Wang, Daisuke Onoshima, Michihiko Aki, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Chemistry, 83, 9, 3528, 3532, May 2011, [Peer-reviewed]
English, Scientific journal

Trafficking and Subcellular Localization of Multiwalled Carbon Nanotubes in Plant Cells
Maged F. Serag, Noritada Kaji, Claire Gaillard, Yukihiro Okamoto, Kazuyoshi Terasaka, Mohammad Jabasini, Manabu Tokeshi, Hajime Mizukami, Alberto Bianco, Yoshinobu Baba
ACS Nano, 5, 1, 493, 499, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Online transient isotachophoresis concentration by the pseudo-terminating electrolyte buffer for the separation of DNA-aptamer and its thrombin complex in poly(methyl methacrylate) microchip
Jun Wang, Yong Zhang, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analyst, 136, 6, 1142, 1147, 2011, [Peer-reviewed]
English, Scientific journal

Thermal lens detection device
Kazuma Mawatari, Toshinori Ohashi, Tomohiko Ebata, Manabu Tokeshi, Takehiko Kitamori
Lab on a Chip, 11, 17, 2990, 2993, 2011, [Peer-reviewed]
English, Scientific journal

A touch-and-go lipid wrapping technique in microfluidic channels for rapid fabrication of multifunctional envelope-type gene delivery nanodevices
Katsuma Kitazoe, Jun Wang, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Kentaro Kogure, Hideyoshi Harashima, Yoshinobu Baba
Lab on a Chip, 11, 19, 3256, 3262, 2011, [Peer-reviewed]
English, Scientific journal

Microfluidic baker's transformation device for three-dimensional rapid mixing
Takao Yasui, Yusuke Omoto, Keiko Osato, Noritada Kaji, Norikazu Suzuki, Toyohiro Naito, Masaki Watanabe, Yukihiro Okamoto, Manabu Tokeshi, Eiji Shamoto, Yoshinobu Baba
Lab on a Chip, 11, 19, 3356, 3360, 2011, [Peer-reviewed]
English, Scientific journal

Aqueous Phase Synthesized CdSe Nanoparticles with Well-Defined Numbers of Constituent Atoms
Yeon-Su Park, Andriy Dmytruk, Igor Dmitruk, Atsuo Kasuya, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Journal of Physical Chemistry C, 114, 44, 18834, 18840, Nov. 2010, [Peer-reviewed]
English, Scientific journal

DNA separation by cholesterol-bearing pullulan nanogels
Keisuke Kondo, Noritada Kaji, Sayaka Toita, Yukihiro Okamoto, Manabu Tokeshi, Kazunari Akiyoshi, Yoshinobu Baba
Biomicrofluidics, 4, 3, 032210-1, 10, Sep. 2010, [Peer-reviewed]
English, Scientific journal

Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells
Hiroshi Yukawa, Yukimasa Kagami, Masaki Watanabe, Koichi Oishi, Yoshitaka Miyamoto, Yukihiro Okamoto, Manabu Tokeshi, Noritada Kaji, Hirofumi Noguchi, Kenji Ono, Makoto Sawada, Yoshinobu Baba, Nobuyuki Hamajima, Shuji Hayashi
Biomaterials, 31, 14, 4094, 4103, May 2010, [Peer-reviewed]
English, Scientific journal

A Probe Containing Two Base-discriminating Fluorescent (BDF) Nucleosides for SNP Typing
Yasuko Yoshida, Kousuke Niwa, Kazunari Yamada, Manabu Tokeshi, Yoshinobu Baba, Yoshio Saito, Akimitsu Okamoto, Isao Saito
Chemistry Letters, 39, 2, 116, 117, Feb. 2010, [Peer-reviewed]
English, Scientific journal

Size-Selective Growth and Stabilization of Small CdSe Nanoparticles in Aqueous Solution
Yeon-Su Park, Andriy Dmytruk, Igor Dmitruk, Atsuo Kasuya, Motohiro Takeda, Noriaki Ohuchi, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
ACS Nano, 4, 1, 121, 128, Jan. 2010, [Peer-reviewed]
English, Scientific journal

Development of an Easy and High-Throughput Cell Assay System with a Culture Chip and an Assay Chip
Kanako Sugiura, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
IEEJ Transactions on Sensors and Micromachines, 130, 10, 471, 475, Institute of Electrical Engineers of Japan (IEE Japan), 2010, [Peer-reviewed]
Japanese, Scientific journal

Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay
Mai Ikami, Ayako Kawakami, Masaya Kakuta, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Lab on a Chip, 10, 24, 3335, 3340, 2010, [Peer-reviewed]
English, Scientific journal

Nanopillar, nanoball, and nanofibers for highly efficient analysis of biomolecules
Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
Chemical Society Reviews, 39, 3, 948, 948, Royal Society of Chemistry (RSC), 2010, [Peer-reviewed]
English, Scientific journal

Exceeding 20 000-fold concentration of protein by the on-line isotachophoresis concentration in poly(methyl methacrylate) microchip
Jun Wang, Yong Zhang, Molhamad Reza Mohamadi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Electrophoresis, 30, 18, 3250, 3256, Sep. 2009, [Peer-reviewed]
English, Scientific journal

Cell separation by the combination of microfluidics and optical trapping force on a microchip
Masaya Murata, Yukihiro Okamoto, Yeon-Su Park, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical and Bioanalytical Chemistry, 394, 1, 277, 283, May 2009, [Peer-reviewed]
English, Scientific journal

Microchip-based homogeneous immunoassay using fluorescence polarization spectroscopy.
Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Lab on a Chip, 9, 7, 966, 71, 07 Apr. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal, We have realized fluorescence polarization immunoassay (FPIA) on a microchip in about 1 minute. FPIA is a homogeneous competitive immunoassay which is based on measuring fluorescence polarization after competitive binding of an analyte and a tracer to an antibody. We constructed a microfluidic FPIA system composed of a newly designed microchip, a laser, a CCD camera and an optical microscope with two specially installed polarizers-one fixed and one rotatable. Theophylline, a typical small drug molecule, was used as a model analyte. Theophylline and fluorescence-labeled theophylline were introduced through different inlets and combined in a 100 microm-wide microchannel where anti-theophylline antibody was added. To optimize the microchip design for FPIA, we investigated the diffusion time of theophylline and the mixing time of theophylline and antibody in this channel, which were 6 s and 36 s, respectively. We successfully carried out a quantitative analysis of theophylline in serum near the therapeutic range in 65 s. In FPIA, a larger tracer-antibody complex emits more polarized fluorescence than the tracer, and therefore, by increasing the antigen concentration in a sample, more polarization relaxation is observed since the tracer-antibody complex concentration is decreased and the tracer concentration is increased. Tracer binding to an antibody is directly measured by spectroscopic techniques without any separation process.This microchip-based FPIA is very simple and rapid, unlike microchip-based heterogeneous immunoassay, because it does not require several processes such as washing and reflowing and immobilizing of antibodies or antigens in the channel. In the future, microchip-based FPIA should find frequent use for point-of-care testing in the clinical field, where conventional FPIA has been used for laboratory tests.

Simultaneous Separation, Metering, and Dilution of Plasma from Human Whole Blood in a Microfluidic System
Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Chemistry, 81, 8, 3194, 3198, Apr. 2009, [Peer-reviewed]
English, Scientific journal

Phase separation of gas-liquid and liquid-liquid microflows in microchips
Arata Aota, Kazuma Mawatari, Susumu Takahashi, Teruki Matsumoto, Kazuteru Kanda, Ryo Anraku, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
Microchimica Acta, 164, 3-4, 249, 255, Mar. 2009, [Peer-reviewed]
English, Scientific journal

Microchip-based homogeneous immunoassay using a cloned enzyme donor.
Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Sciences, 25, 2, 149, 51, Feb. 2009, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme beta-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 microg mL(-1). Our CEDIA using a microfluidic device was very simple and rapid, unlike microchip-based heterogeneous immunoassays and CEDIA on a well-type microchip.

Luminescence of Cup-Stacked Carbon Nanotubes and Its Application to Microchip Electrophoresis
Yousuke Inoue, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
BUNSEKI KAGAKU, 58, 6, 517, 521, Japan Society for Analytical Chemistry, 2009, [Peer-reviewed]
Japanese, Scientific journal

A micro-ELISA system for the rapid and sensitive measurement of total and specific immunoglobulin E and clinical application to allergy diagnosis
Toshinori Ohashi, Kazuma Mawatari, Kae Sato, Manabu Tokeshi, Takehiko Kitamori
Lab on a Chip, 9, 7, 991, 995, 2009, [Peer-reviewed]
English, Scientific journal

Quantum Dots for Labeling Adipose Tissue-Derived Stem Cells
Hiroshi Yukawa, Shogo Mizufune, Chiharu Mamori, Yukimasa Kagami, Koichi Oishi, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Hirofumi Noguchi, Yoshinobu Baba, Michinari Hamaguchi, Nobuyuki Hamajima, Shuji Hayashi
Cell Transplantation, 18, 5-6, 591, 599, 2009
English, Scientific journal

Nanotechnologies in the biosciences
Manabu Tokeshi, Yoshinobu Baba
Analytical and Bioanalytical Chemistry, 391, 8, 2693, 2694, Aug. 2008, [Peer-reviewed]
Scientific journal

On-chip fabrication of mutifunctional envelope-type nanodevices for gene delivery
Hiroshi Kuramoto, Yeon-Su Park, Noritada Kaji, Manabu Tokeshi, Kentaro Kogure, Yasuo Shinohara, Hideyoshi Harashima, Yoshinobu Baba
Analytical and Bioanalytical Chemistry, 391, 8, 2729, 2733, Aug. 2008
English, Scientific journal

A viscosity-tunable polymer for DNA separation by microchip electrophoresis
Daisuke Kuroda, Yong Zhang, Jun Wang, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical and Bioanalytical Chemistry, 391, 7, 2543, 2549, Aug. 2008
English, Scientific journal

Flowing thermal lens micro-flow velocimeter
Yoshikuni Kikutani, Kazuma Mawatari, Kenji Katayama, Manabu Tokeshi, Takashi Fukuzawa, Mitsuo Kitaok, Takehiko Kitamori
Sensors and Actuators B-Chemical, 133, 1, 91, 96, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Highly sensitive double-fluorescent dye staining method on microchip electrophoresis for analysis of milk proteins
Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Electrophoresis, 29, 12, 2533, 2538, Jun. 2008, [Peer-reviewed]
English, Scientific journal

Microchip analysis of plant glucosinolates
Maged Fouad, Mohammad Jabasini, Noritada Kaji, Kazuyoshi Terasaka, Manabu Tokeshi, Hajime Mizukami, Yoshinobu Baba
Electrophoresis, 29, 11, 2280, 2287, Jun. 2008, [Peer-reviewed]
English, Scientific journal

Numerical analysis of thermal lens effect for sensitive detection on microchips
Ryo Anraku, Kazuma Mawatari, Manabu Tokeshi, Masatoshi Nara, Takahiro Asai, Akihiko Hattori, Takehiko Kitamori
Electrophoresis, 29, 9, 1895, 1901, May 2008, [Peer-reviewed]
English, Scientific journal

Accurate quantitation of salivary and pancreatic amylase activities in human plasma by microchip electrophoretic separation of the substrates and hydrolysates coupled with immunoinhibition
Eiki Maeda, Masatoshi Kataoka, Shouki Yatsushiro, Kazuaki Kajimoto, Mami Hino, Noritada Kaji, Manabu Tokeshi, Mika Bando, Jun-Ichi Kido, Mitsuru Ishilkawa, Yasuo Shinohara, Yoshinobu Baba
Electrophoresis, 29, 9, 1902, 1909, May 2008
English, Scientific journal

Rinse and evaporation coating of poly(methyl methacrylate) microchip for separation of sodium dodecyl sulfate-protein complex
Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Journal of Chromatography A, 1192, 2, 289, 293, May 2008
English, Scientific journal

Rolling circle amplification and circle-to-circle amplification of a specific gene integrated with electrophoretic analysis on a single chip
Laili Mahmoudian, Noritada Kaji, Manabu Tokeshi, Mats Nilsson, Yoshinobu Baba
Analytical Chemistry, 80, 7, 2483, 2490, Apr. 2008
English, Scientific journal

Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification
Laili Mahmoudian, Jonas Melin, Mohamad Reza Mohamadi, Keiko Yamada, Michio Ohta, Noritada Kaji, Manabu Tokeshi, Mats Nilsson, Yoshinobu Baba
Analytical Sciences, 24, 3, 327, 332, 10 Mar. 2008, [Peer-reviewed]
English, Scientific journal

Poly(methylmethacrylate) microchip electrophoresis of proteins using linear-poly(acrylamide) solutions as separation matrix
Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Sciences, 24, 3, 321, 325, Mar. 2008
English, Scientific journal

Quantitative determination of amino acids in functional foods by microchip electrophoresis
Hiroko Ueno, Jun Wang, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Journal of Separation Science, 31, 5, 898, 903, Mar. 2008
English, Scientific journal

Nuclease tolerant FRET probe based on DNA-quantum dot conjugation
Daisuke Onoshima, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Sciences, 24, 2, 181, 183, Feb. 2008
English, Scientific journal

Dynamic cross-linking effect of Mg2+ to enhance sieving properties of low-viscosity poly(vinylpyrrolidone) solutions for microchip electrophoresis of proteins
Mohamad Reza Mohamadi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Chemistry, 80, 1, 312, 316, Jan. 2008
English, Scientific journal

Channel wall coating on a poly-(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size-based protein separation.
Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Electrophoresis, 28, 24, 4582, 9, Dec. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal, We demonstrate channel wall coating using a cellulose derivative on a poly-(methyl methacrylate) (PMMA) CE microchip to eliminate EOF disturbing protein separation. The channel walls were modified by preconditioning with a solution containing the cellulose derivative and then thermally evaporating the solution to produce hydrophilic channel walls which prevent adsorption of analytes via a hydrophobic interaction. When the PMMA substrate was coated with the cellulose derivative hydroxypropylmethylcellulose (HPMC) 90SH, the water contact angle on the coated substrate was decreased (up to 15 degrees ) and EOF was significantly suppressed (up to 4.0 x 10(-6) cm2.V(-1)s(-1)). Three proteins (20.5, 68.0, and 114.6 kDa) were successfully separated on the 0.15% HPMC 90SH-coated channel walls with good reproducibility of migration time (RSD <1.75%) and high efficiency (theoretical plate number per meter: 2.62 x 10(5)).

Dynamic modification of poly(methyl methacrylate) chips using poly(vinyl alcohol) for glycosaminoglycan disaccharide isomer separation.
Yong Zhang, Guichen Ping, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Electrophoresis, 28, 18, 3308, 14, Sep. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal, We describe a microchip electrophoresis (MCE) method for the assay of unsaturated disaccharides of chondroitin sulfates, dermatan sulfates, and hyaluronic acid (HA). Poly(vinyl alcohol) (PVA) could be irreversibly adsorbed onto poly(methyl methacrylate) (PMMA) substrates and this approach was applicable for dynamic coating. The characteristics of the PMMA surface with PVA coating were evaluated in terms of the wettability, EOF, and adsorption of 2-aminoacridone (AMAC)-labeled disaccharide. The water contact angle decreased from 73 degrees on a pristine PMMA surface to 37.5 degrees on a PVA-coated surface, indicating that the PVA coating increased hydrophilicity. EOF was reduced approximately twofold and was relatively stable. Scanning electron microscopy and fluorescence microscopy images showed that adsorption of AMAC-labeled disaccharides was dramatically suppressed. Using the PVA coating, baseline separation of two pairs of glycosaminoglycan (GAG) disaccharide isomers, DeltaDi-diS(B)/DeltaDi-diS(D) and DeltaDi-0S/DeltaDi-HA, was achieved in Tris-borate buffer within 130 s by MCE.

Nanobiotechnology: quantum dots in bioimaging
Yong Zhang, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Expert Review of Proteomics, 4, 4, 565, 572, Informa UK Limited, Aug. 2007, [Peer-reviewed], [Invited]
English, Scientific journal

Online preconcentration by transient isotachophoresis in linear polymer on a poly(methyl methacrylate) microchip for separation of human serum albumin immunoassay mixtures.
Mohamad Reza Mohamadi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Chemistry, 79, 10, 3667, 72, 15 May 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal, Online preconcentration of human serum albumin (HSA) and its immunocomplex with a monoclonal antibody by on-chip transient isotachophoresis is reported. An 800-fold signal enhancement was achieved following the preconcentration on standard cross-channel microchips made of poly (methyl methacrylate). Sample injection, preconcentration, and separation were done continuously and controlled solely by a sequential voltage switching program. The preconcentration was followed by on-chip nondenaturing gel electrophoresis in methylcellulose solution. The method was applied to microchip electrophoresis immunoassay of HSA. Baseline separation of HSA and its immunocomplex was achieved in 25 s in the first 1 cm of the microchannel. In a direct immunoassay, the minimum detectable concentration of fluorescent labeled HSA by laser-induced fluorescence detection was 7.5 pM.

Evaluation of amplified cRNA targets for oligonucleotide microarrays.
Akihiro Sawada, Shogo Mizufune, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical and Bioanalytical Chemistry, 387, 8, 2645, 54, Apr. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal, Due to their hybridization specificity and capacity for systematic gene discovery, oligonucleotide-based microarray platforms offer numerous advantages over the cDNA microarrays currently widely used for comprehensive analysis of gene expression. Although fluorescently labeled amplified cRNA generated by T7 transcription is generally used in oligonucleotide microarrays, the feasibility of this combination (and that of cDNA microarrays) is yet to be studied systematically. In this paper, we performed a comparative study using a direct labeling method and T7 amplification to evaluate amplified cRNA targets for oligonucleotide microarrays. The efficiency of incorporation of Cy3- and Cy5-CTP into the target preparations, the reproducibility and the number of genes detected were investigated for each labeling approach and compared. The 12 genes that showed different expression profiles in the two labeling methods were evaluated by quantitative real-time PCR. In the 60-mer oligonucleotide microarray, amplified cRNA targets prepared by the T7 amplification method showed higher reproducibility and reliability than targets prepared by the direct labeling method in a comparative analysis of gene expression. This result also suggests the importance of fragmenting cRNA down to lengths of 50-200 bases before the hybridization process.

Enhanced electrophoretic resolution of monosulfate glycosaminoglycan disaccharide isomers on poly(methyl methacrylate) chips.
Yong Zhang, Guichen Ping, Bingmei Zhu, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Electrophoresis, 28, 3, 414, 21, Feb. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal, To improve the separation of monosulfate glycosaminoglycan disaccharide isomers by microchip electrophoresis, we found that addition of 1,4-dioxane (DO) dramatically improved analyte resolution, probably due to solvation effects. Methylcellulose (MC) was tested for the ability to suppress EOF and analyte adsorption to the chip. To improve analyte resolution, buffer pH, beta-CD, and DO were systematically investigated. Fast separation was achieved by increasing the electric field strength, and field-amplified sample stacking occurred with increasing buffer concentrations. Therefore, based on our findings, we describe an efficient method for the separation of monosulfate and trisulfate unsaturated disaccharides (DeltaDi-UA2S, DeltaDi-4S, DeltaDi-6S, and DeltaDi-triS) derivatized with 2-aminoacridone hydrochloride. A mixture of monosulfate disaccharide isomers (DeltaDi-UA2S, DeltaDi-4S, and DeltaDi-6S) was baseline-separated within 75 s on a poly(methyl methacrylate) chip using a mixed buffer (DO/running buffer 57:43 v:v), 0.5% MC, pH 6.81, with an E(sep) of 558 V/cm. The theoretical plate was in the range of 5 x 10(5) to 1 x 10(6) m-1.

Highly Sensitive Detection of Non-Labeled Peptides Using UV Excitation Thermal Lens Microscope/Liquid Chromatography
Shinichiro HIKI, Manabu TOKESHI, Masaya KAKUTA, Kazuma MAWATARI, Yoshikuni KIKUTANI, Kiichi SATO, Akihide HIBARA, Kiyohito SHIMURA, Naoyuki UCHIDA, Takehiko KITAMORI
BUNSEKI KAGAKU, 56, 1, 1, 7, 2007, [Peer-reviewed]
Japanese, Scientific journal

Quantum dots for single bio-molecule imaging.
Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Analytical Sciences, 23, 1, 21, 4, Springer Science and Business Media LLC, Jan. 2007, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The emerging nanomaterial, quantum dots or QDs, offers numerous potential applications in the biological area. As cell labeling probes, QDs become now an alternative of existing organic fluorescent dyes and fluorescent proteins. In this short review, we cover typical and successful applications of QDs as fluorescent probes in cell labeling and genomic diagnosis. As a future important application, biomolecular detection at a single molecule level utilizing QDs is also discussed.

Development of the microchip-based repeatable immunoassay system for clinical diagnosis
M. Kakuta, H. Takahashi, S. Kazuno, K. Murayama, T. Ueno, M. Tokeshi
Measurement Science and Technology, 17, 12, 3189, 3194, Institute of Physics Publishing, 01 Dec. 2006, [Peer-reviewed]
English, Scientific journal

Pressure-driven flow control system for nanofluidic chemical process
Eiichiro Tamaki, Akihide Hibara, Haeng-Boo Kim, Manabu Tokeshi, Takehiko Kitamori
Journal of Chromatography A, 1137, 2, 256, 262, Dec. 2006, [Peer-reviewed]
English, Scientific journal

Study of water properties in nanospace.
Noritada Kaji, Ryo Ogawa, Akio Oki, Yasuhiro Horiike, Manabu Tokeshi, Yoshinobu Baba
Analytical and Bioanalytical Chemistry, 386, 3, 759, 64, Oct. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal, Here we report an anomalous behavior of water, especially its viscosity and hydrodynamic flow, in a nanometer-confined space. As a typical model of a nanometer-confined space, the nanopillar chip, which was developed for DNA size-based separation was used, and single-particle tracking (SPT) technique was applied to investigate water viscosity and hydrodynamic flow in the nanopillar chip. The diffusion coefficients of nanospheres were almost one-third of the theoretical value derived from the Stokes-Einstein equation. This result gave indirect proof that water viscosity in a nanometer-confined space is higher than in a bulk solution. In order to improve resolution and throughput of the nanopillar chip for DNA separation, these potential factors affecting performance should be seriously considered.

Microchip-based liquid–liquid extraction for gas-chromatography analysis of amphetamine-type stimulants in urine
Hajime Miyaguchi, Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara, Hiroyuki Inoue, Takehiko Kitamori
Journal of Chromatography A, 1129, 1, 105, 110, Sep. 2006, [Peer-reviewed]
English, Scientific journal

Electrophoretic behavior of plasmid DNA in the presence of various intercalating dyes.
Fuquan Dang, Wenhao Li, Lihua Zhang, Mohammad Jabasini, Tatsuhiro Ishida, Hiroshi Kiwada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Journal of Chromatography. A, 1118, 2, 218, 25, 23 Jun. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal, In the present study, the electrophoretic behavior of linear, supercoiled and nicked circular plasmid DNA in the presence of various intercalating dyes was characterized using pGL3 plasmid DNA as a model. The enzymatic digestion of pGL3 plasmid DNA with HindIIIwas monitored by capillary electrophoresis coupled with laser-induced fluorescence detection (CE-LIF). Nicked circular plasmid DNA was found to be relatively sensitive to enzymes, and was almost digested into the linear conformer after 10-min incubation, indicating that nicked circular plasmid DNA has little chance of targeting and entering the cell nucleus. Partly digested plasmid DNA containing only linear and supercoiled conformers can be used as a standard to confirm the migration order of plasmid DNA. In methylcellulose (MC) solution with YO-PRO-1 or YOYO-1, linear plasmid DNA eluted first, followed by supercoiled and nicked plasmid DNA, and nicked plasmid DNA eluted as a broad peak. With SYBR Green 1, nicked plasmid DNA eluted first as three sharp peaks, followed by linear and supercoiled plasmid DNA. The nuclear plasmid DNA from two transfected cell lines was successfully analyzed using the present procedure. Similar results were obtained with an analysis time of seconds using microchip electrophoresis with laser-induced fluorescence detection (mu-CE-LIF). To our knowledge, these results represent the first reported analysis of nuclear plasmid DNA from transfection cells by CE-LIF or mu-CE-LIF without pre-preparation, suggesting that the present procedure is a promising alternative method for evaluating transfection efficiency of DNA delivery systems.

Quantitative detection and fixation of single and multiple gold nanoparticles on a microfluidic chip by thermal lens microscope
Kazuma Mawatari, Manabu Tokeshi, Takehiko Kitamori
Analytical Sciences, 22, 5, 781, 784, May 2006, [Peer-reviewed]
English, Scientific journal

Circular dichroism thermal lens microscope for sensitive chiral analysis on microchip
Masayo Yamauchi, Kazuma Mawatari, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
Analytical Chemistry, 78, 8, 2646, 2650, 15 Apr. 2006, [Peer-reviewed]
English, Scientific journal

Monitoring of intercellular messengers released from neuron networks cultured in a microchip
Kiichi Sato, Akiko Egami, Tamao Odake, Manabu Tokeshi, Makoto Aihara, Takehiko Kitamori
Journal of Chromatography A, 1111, 2, 228, 232, 14 Apr. 2006, [Peer-reviewed]
English, International conference proceedings

Liquid Filling Method for Nanofluidic Channels Utilizing the High Solubility of CO2
Eiichiro Tamaki, Akihide Hibara, Haeng-Boo Kim, Manabu Tokeshi, Takeshi Ooi, Masayuki Nakao, Takehiko Kitamori
Analytical Sciences, 22, 4, 529, 532, 14 Apr. 2006, [Peer-reviewed]
English, Scientific journal

Total reflection X-ray fluorescence analysis with chemical microchip
Kouichi Tsuji, Yousuke Hanaoka, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
Spectrochimica Acta - Part B Atomic Spectroscopy, 61, 4, 389, 392, Apr. 2006
English, Scientific journal

Low viscous separation media for genomics and proteomics analysis on microchip electrophoresis system.
Mohammad Jabasini, Yuji Murakami, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Biological and Pharmaceutical Bulletin, 29, 4, 595, 604, Pharmaceutical Society of Japan, Apr. 2006, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Microchip electrophoresis has widely grown during the past few years, and it has showed a significant result as a strong separation tool for genomic as well as proteomic researches. To enhance and expand the role of microchip electrophoresis, several studies have been proposed especially for the low viscous separation media, which is an important factor for the success of microchip with its narrow separation channels. In this paper we show an overview for the done researches in the field of low viscous media developed for the use in microchip electrophoresis. For genomic separation studies polyhydroxy additives have been used enhance the separation of DNA at low polymer concentration of HPMC (Hydroxypropylmethyl cellulose) which could keep the viscosity low. Mixtures of poly(ethylene oxide) as well as Hydroxyporpyl cellulose have been successfully introduced for chip separation. Furthermore high molecular mass polyacrylamides at low concentrations have been studied for DNA separation. A mixture of polymer nanoparticle with conventional polymers could show a better resolution for DNA at low concentration of the polymer. For the proteomic field isoelectric focusing on chip has been well overviewed since it is the most viscous separation media which is well used for the protein separation. The different types of isoelectric focusing such as the ampholyte-free type, the thermal type as well as the ampholyte-depended type have been introduced in this paper. Isoelectric focusing on chip with its combination with sodium dodecyl sulfate (SDS) page or free solution could give a better separation. Several application for this low viscous separation medias for either genomic or proteomic could clearly show the importance of this field.

Rapid analysis of oligosaccharides derived from glycoproteins by microchip electrophoresis.
Fuquan Dang, Kazuaki Kakehi, Kazuki Nakajima, Yasuo Shinohara, Mitsuru Ishikawa, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Journal of Chromatography. A, 1109, 2, 138, 43, 24 Mar. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal, A novel method for fast profiling of complex oligosaccharides released from glycoproteins based on microchip electrophoresis (mu-CE) is presented here. The characterization of separation conditions, i.e., the composition, concentration and pH of running buffer as well as the applied voltage, has been performed using maltose (G2), cellobiose ( G2'), maltriose (G3) and panose (G3') as oligosaccharide isomer models. In mu-CE, much better separation of oligosaccharide isomers and oligosaccharide ladder was obtained in phosphate buffer than in borate buffer over a wide pH range. Under optimal conditions, high-performance separation of the N-linked complex oligosaccharides released from ribonuclease B, fetuin, alpha1-acid glycoprotein (AGP) and IgG was achieved using polymethylmethacrylate (PMMA) microchips with an effective separation channel of 30 mm. These results represent the first reported analysis of the N-linked oligosaccharides derived from glycoproteins by mu-CE, indicating that the present mu-CE-based method is a promising alternative for characterization of the N-linked oligosaccharides in glycoproteins.

Development of the innovative nuclide separation system for high-level radioactive waste using microchannel chip—extraction behavior of metal ions from aqueous phase to organic phase in microchannel
Hiroyasu Hotokezaka, Manabu Tokeshi, Masayuki Harada, Takehiko Kitamori, Yasuhisa Ikeda
Progress in Nuclear Energy, 48, 2, 187, 187, Elsevier BV, Mar. 2006, [Peer-reviewed]
English, Scientific journal

Nanotechnology for genomics & proteomics
Mohamad Reza Mohamadi, Laili Mahmoudian, Noritada Kaji, Manabu Tokeshi, Hiroshi Chuman, Yoshinobu Baba
Nano Today, 1, 1, 38, 45, Elsevier BV, Feb. 2006, [Peer-reviewed], [Invited]
English, Scientific journal

Miniaturized thermal lens and fluorescence detection system for microchemical chips
Masayo Yamauchi, Manabu Tokeshi, Jun Yamaguchi, Takashi Fukuzawa, Akihiko Hattori, Akihide Hibara, Takehiko Kitamori
Journal of Chromatography A, 1106, 1-2, 89, 93, Feb. 2006, [Peer-reviewed]
English, Scientific journal

Radiation Degradation of Microchemical Chips and Capillary Tubes by Gamma-Ray Irradiation
Hidematsu IKEDA, Manabu TOKESHI, Hiroyasu HOTOKEZAKA, Yasuhisa IKEDA, Takehiko KITAMORI
Transactions of the Atomic Energy Society of Japan, 5, 3, 209, 220, Atomic Energy Society of Japan, 2006, [Peer-reviewed]
Japanese, Scientific journal

“UV-excitation thermal lens microscope for non-labeled and ultrasensitive detection of non-fluorescent molecules
S. Hiki, K.Mawatari, A.Hibara, M.Tokeshi, T.Kitamori
Analytical Chemistry, 8, 78, 2859, 2863, 2006, [Peer-reviewed]
English, Scientific journal

Rapid Multiplexing and Simultaneous Detection of Human Spermatogenetic Failure with a 12 Lane Microchip Electrophoresis System
Mohammad Jabasini, Ashraf Abdulazim Ewis, Maged Fouad, Fuquan Dang, Guichen Ping, Toshikatsu Shinka, Yutaka Nakahori, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
Biological and Pharmaceutical Bulletin, 29, 7, 1487, 1489, Pharmaceutical Society of Japan, 2006, [Peer-reviewed]
English, Scientific journal

Continuous‐Flow Chemical Processing in Three‐Dimensional Microchannel Network for On‐Chip Integration of Multiple Reactions in a Combinatorial Mode
Yoshikuni Kikutani, Masaharu Ueno, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori
QSAR & Combinatorial Science, 24, 6, 742, 757, Wiley, 16 Aug. 2005, [Peer-reviewed], [Invited]
English, Scientific journal, Abstract

Chemical syntheses using microchemical chips have been intensively investigated in recent years, but they have still not fully reached the stage of practical use. Combinatorial synthesis has been expected to be one of their useful applications. Benefits of using microchips, such as reduction in quantity of reagents and wastes, are thought to be suited for combinatorial syntheses, where numerous variations of products have to be synthesized each in small amounts. There are two modes of combinatorial syntheses using microchemical chips. One is sequential synthesis, in which reactant solutions are introduced sequentially in various combinations to a reaction microchannel. The other is parallel synthesis, where the number of reaction channels is the same as the number of final products, and starting materials are distributed to all the reaction channels in different combinations. Sequential synthesis has been considered as the more favorable way, because, especially when libraries of starting materials are large, numerous microchannels and complicated pipings for distribution of reagents to the channels are required for the parallel mode. Therefore, research on parallel microcombinatorial syntheses has been done only rarely, whereas there have been many reports of sequential microcombinatorial syntheses. Against this trend, our research group has taken an alternative path and tried to integrate both parallel reaction microchannels onto a glass microchip and channels for distributing reactant solutions to each reaction channel in a combinatorial mode. Microchips fabricated by laminating multiple layers of glass substrates have been used to construct three‐dimensional microchannel networks necessary for mixing reagent flows in different combinations. We utilized low Reynolds number multi‐phase laminar flow and developed micro‐unit operations (MUOs). By combining MUOs in the multi‐phase continuous laminar flows, microchemical systems were constructed. We called this micro‐integration methodology “continuous‐flow chemical processing (CFCP)”, and by using it, a sequence of various operations in chemical syntheses could be integrated on a microchemical chip. Not just simple mixing of two reagents, which was most commonly done in microreactors, but also multi‐step processes including other chemical operations, such as extraction and separation, could be carried out via CFCP. In the first part of this short review article, we explain CFCP with an example. Then, a microchemical chip with a three‐dimensional microchannel network for a parallel reaction system is introduced. Finally, we present a case in which CFCP was realized in a three‐dimensional microchannel network.

System for high-level radioactive waste using microchannel chip - Extraction behavior of metal ions from aqueous phase to organic phase in microchannel
Hiroyasu Hotokezaka, Manabu Tokeshi, Masayuki Harada, Takehiko Kitamori, Yasuhisa Ikeda
Progress in Nuclear Energy, 47, 1-4, 439, 447, Jul. 2005, [Peer-reviewed]
English, International conference proceedings

Effect of korteweg stress in miscible liquid two-layer flow in a microfluidic device
Y. Sugii, K. Okamoto, A. Hibara, M. Tokeshi, T. Kitamori
Journal of Visualization, 8, 2, 117, 124, Jun. 2005, [Peer-reviewed]
Scientific journal

Surface modification method of microchannels for gas-liquid two-phase flow in microchips
Akihide Hibara, Shinobu Iwayama, Shinya Matsuoka, Masaharu Ueno, Yoshikuni Kikutani, Manabu Tokeshi, Takehiko Kitamori
Analytical Chemistry, 77, 3, 943, 947, 01 Feb. 2005, [Peer-reviewed]
English, Scientific journal

Optimization of an Interface Chip for Coupling Capillary Electrophoresis with Thermal Lens Microscopic Detection
Kenji Uchiyama, Manabu Tokeshi, Yoshikuni Kikutani, Akihiko Hattori, Takehiko Kitamori
Analytical Sciences, 21, 1, 49, 52, 25 Jan. 2005, [Peer-reviewed]
English, Scientific journal

Continuous flow chemical processing on a microchip using microunit operations and a multiphase flow network
Manabu Tokeshi, Takehiko Kitamori
Progress in Nuclear Energy, 47, 1-4, 434, 438, Jan. 2005, [Peer-reviewed]
English, Scientific journal

Tunable thermal lens spectrometry utilizing microchannel-assisted thermal lens spectrometry
Eiichiro Tamaki, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
Lab on a Chip, 5, 2, 129, 129, 2005, [Peer-reviewed]
English, Scientific journal

Development of a Microchip-Based Bioassay System Using Cultured Cells　　　　　　　　　　　　　　　
Makiko Goto, Kiichi Sato, Atsushi Murakami, Manabu Tokeshi, Takehiko Kitamori
Analytical Chemistry, 77, 2125, 2131, 2005

Thermal Lens Micro Optical Systems
Manabu Tokeshi, Jun Yamaguchi, Akihiko Hattori, Takehiko Kitamori
Analytical Chemistry, 77, 2, 626, 630, 08 Dec. 2004, [Peer-reviewed]
English, Scientific journal

Rapid proton diffusion in microfluidic devices by means of micro-LIF technique
K. Shinohara, Y. Sugii, A. Hibara, M. Tokeshi, T. Kitamori, K. Okamoto
Experiments in Fluids, 38, 1, 117, 122, 30 Nov. 2004, [Peer-reviewed]
English, Scientific journal

High-speed micro-PIV measurements of transient flow in microfluidic devices
Kyosuke Shinohara, Yasuhiko Sugii, Arata Aota, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori, Koji Okamoto
Measurement Science and Technology, 15, 10, 1965, 1970, 21 Aug. 2004, [Peer-reviewed]
English, Scientific journal

Measurement of pH field of chemically reacting flow in microfluidic devices by laser-induced fluorescence
Kyosuke Shinohara, Yasuhiko Sugii, Koji Okamoto, Haruki Madarame, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
Measurement Science and Technology, 15, 5, 955, 960, 21 Apr. 2004, [Peer-reviewed]
English, Scientific journal

Peer Reviewed: Thermal Lens Microscopy and Microchip Chemistry
Takehita Kitamori, Manabu Tokeshi, Akihide Hibara, Kiichi Sato
Analytical Chemistry, 76, 3, 52 A, 60 A, 01 Feb. 2004, [Peer-reviewed]
English, Scientific journal

Microchip-based enzyme-linked immunosorbent assay (microELISA) system with thermal lens detection
Kiichi Sato, Maho Yamanaka, Tomokazu Hagino, Manabu Tokeshi, Hiroko Kimura, Takehiko Kitamori
Lab on a Chip, 4, 6, 570, 575, Royal Society of Chemistry, 2004, [Peer-reviewed]
English, Scientific journal

Micro wet analysis system using multi-phase laminar flows in three-dimensional microchannel networkElectronic supplementary information (ESI) available: illustration and microscopic view of two-phase laminar flow. See http://www.rsc.org/suppdata/lc/b4/b400233d/
Yoshikuni Kikutani, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori
Lab on a Chip, 4, 4, 328, 328, 2004, [Peer-reviewed]
English, Scientific journal

Spectroscopic Analysis of Liquid/Liquid Interfaces in Multiphase Microflows
Akihide Hibara, Masaki Nonaka, Manabu Tokeshi, Takehiko Kitamori
Journal of the American Chemical Society, 125, 49, 14954, 14955, 15 Nov. 2003, [Peer-reviewed]
English, Scientific journal

Chemical processing on microchips for analysis, synthesis, and bioassay
Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Takehiko Kitamori
ELECTROPHORESIS, 24, 21, 3583, 3594, Wiley, 06 Nov. 2003, [Peer-reviewed], [Invited]
English, Scientific journal, Abstract

This review describes our recent research on miniaturization of chemical systems. We have developed a miniaturization methodology based on pressure‐driven multiphase laminar flow and a highly sensitive detection tool, the thermal lens microscope. Some representative applications of the methodology in the fields of analysis, synthesis, and bioassay are described.

Optimisation of thermal lens microscopic measurements in a microchip
Mikhail A. Proskurnin, Maksim N. Slyadnev, Manabu Tokeshi, Takehiko Kitamori
Analytica Chimica Acta, 480, 1, 79, 95, Elsevier, 17 Mar. 2003, [Peer-reviewed]
English, Scientific journal

Microchip-based chemical and biochemical analysis systems
Kiichi Sato, Akihide Hibara, Manabu Tokeshi, Hideaki Hisamoto, Takehiko Kitamori
Advanced Drug Delivery Reviews, 55, 3, 379, 391, Elsevier BV, Feb. 2003, [Peer-reviewed], [Invited]
English, Scientific journal

Microchannel-assisted thermal-lens spectrometry for microchip analysis
Eiichiro Tamaki, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
Journal of Chromatography A, 987, 1-2, 197, 204, Feb. 2003, [Peer-reviewed]
English, Scientific journal

Integration of Chemical and Biochemical Analysis Systems into a Glass Microchip
Kiichi Sato, Akihide Hibara, Manabu Tokeshi, Hideaki Hisamoto, Takehiko Kitamori
Analytical Sciences, 19, 1, 15, 22, Springer Science and Business Media LLC, 11 Jan. 2003, [Peer-reviewed], [Invited]
English, Scientific journal

An interface chip connection between capillary electrophoresis and thermal lens microscope
Kenji Uchiyama, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori
Electrophoresis, 24, 1-2, 179, 184, 10 Jan. 2003, [Peer-reviewed]
English, Scientific journal

Fundamental Study on the Segmented Flow Injection - Multiphase Flow Formation Towards Microchip-Based Multi-Ion Sensing
Hideaki Hisamoto, Takayuki Horiuchi, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
IEEJ Transactions on Sensors and Micromachines, 123, 4, 124, 127, Institute of Electrical Engineers of Japan (IEE Japan), 2003, [Peer-reviewed]
Japanese, Scientific journal

Development of a desktop-sized thermal lens microscope
Shinichiro HIKI, Manabu TOKESHI, Akihide HIBARA, Takehiko KITAMORI
BUNSEKI KAGAKU, 52, 8, 569, 574, 2003, [Peer-reviewed]
Japanese, Scientific journal

Chemicofunctional Membrane for Integrated Chemical Processes on a Microchip
Hideaki Hisamoto, Yuki Shimizu, Kenji Uchiyama, Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara, Takehiko Kitamori
Analytical Chemistry, 75, 2, 350, 354, 13 Dec. 2002, [Peer-reviewed]
English, Scientific journal

Integrated chemical systems on microchips for analysis and assay. Potential future, mobile high-performance detection system for chemical weapons
Yoshikuni Kikutani, Manabu Tokeshi, Kiichi Sato, Takehiko Kitamori
Pure and Applied Chemistry, 74, 12, 2299, 2309, Walter de Gruyter GmbH, 01 Dec. 2002, [Peer-reviewed]
English, International conference proceedings

Nanochannels on a Fused-Silica Microchip and Liquid Properties Investigation by Time-Resolved Fluorescence Measurements
Akihide Hibara, Takumi Saito, Haeng-Boo Kim, Manabu Tokeshi, Takeshi Ooi, Masayuki Nakao, Takehiko Kitamori
Analytical Chemistry, 74, 24, 6170, 6176, 08 Nov. 2002, [Peer-reviewed]
English, Scientific journal

Glass microchip with three-dimensional microchannel network for 2 × 2 parallel synthesis
Yoshikuni Kikutani, Takayuki Horiuchi, Kenji Uchiyama, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori
Lab on a Chip, 2, 4, 188, 192, Royal Society of Chemistry, 01 Nov. 2002, [Peer-reviewed]
English, Scientific journal

Microchip-based immunoassay system with branching multichannels for simultaneous determination of interferon-γ
Kiichi Sato, Maho Yamanaka, Hiroko Takahashi, Manabu Tokeshi, Hiroko Kimura, Takehiko Kitamori
Electrophoresis, 23, 5, 734, 739, Mar. 2002, [Peer-reviewed]
English, Scientific journal

Single-Cell Analysis by a Scanning Thermal Lens Microscope with a Microchip:  Direct Monitoring of Cytochrome c Distribution during Apoptosis Process
Eiichiro Tamaki, Kiichi Sato, Manabu Tokeshi, Kae Sato, Makoto Aihara, Takehiko Kitamori
Analytical Chemistry, 74, 7, 1560, 1564, 21 Feb. 2002, [Peer-reviewed]
English, Scientific journal

Continuous-Flow Chemical Processing on a Microchip by Combining Microunit Operations and a Multiphase Flow Network
Manabu Tokeshi, Tomoko Minagawa, Kenji Uchiyama, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Takehiko Kitamori
Analytical Chemistry, 74, 7, 1565, 1571, 21 Feb. 2002, [Peer-reviewed], [Lead author]
English, Scientific journal

Stabilization of Liquid Interface and Control of Two-Phase Confluence and Separation in Glass Microchips by Utilizing Octadecylsilane Modification of Microchannels
Akihide Hibara, Masaki Nonaka, Hideaki Hisamoto, Kenji Uchiyama, Yoshikuni Kikutani, Manabu Tokeshi, Takehiko Kitamori
Analytical Chemistry, 74, 7, 1724, 1728, 16 Feb. 2002, [Peer-reviewed]
English, Scientific journal

Pile-up glass microreactor
Yoshikuni Kikutani, Akihide Hibara, Kenji Uchiyama, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori
Lab on a Chip, 2, 4, 193, 193, 2002, [Peer-reviewed]
English, Scientific journal

Fast and high conversion phase-transfer synthesis exploiting the liquid–liquid interface formed in a microchannel chip
Hideaki Hisamoto, Takumi Saito, Manabu Tokeshi, Akihide Hibara, Takehiko Kitamori
Chemical Communications, 24, 2662, 2663, 04 Dec. 2001, [Peer-reviewed]
English, Scientific journal

On-Chip Integration of Sequential Ion-Sensing System Based on Intermittent Reagent Pumping and Formation of Two-Layer Flow
Hideaki Hisamoto, Takayuki Horiuchi, Kenji Uchiyama, Manabu Tokeshi, Akihide Hibara, Takehiko Kitamori
Analytical Chemistry, 73, 22, 5551, 5556, 28 Sep. 2001, [Peer-reviewed]
English, Scientific journal

Photothermal Temperature Control of a Chemical Reaction on a Microchip Using an Infrared Diode Laser
Maxim N. Slyadnev, Yuki Tanaka, Manabu Tokeshi, Takehiko Kitamori
Analytical Chemistry, 73, 16, 4037, 4044, 12 Jul. 2001, [Peer-reviewed]
English, Scientific journal

Morphological Dependence of Radiative and Nonradiative Relaxation Energy Balance in Photoexcited Aryl Ether Dendrimers as Observed by Fluorescent and Thermal Lens Spectroscopies
Yuki Wakabayashi, Manabu Tokeshi, Akihide Hibara, Dong-Lin Jiang, Takuzo Aida, Takehiko Kitamori
The Journal of Physical Chemistry B, 105, 19, 4441, 4445, 25 Apr. 2001, [Peer-reviewed]
English, Scientific journal

Determination of Subyoctomole Amounts of Nonfluorescent Molecules Using a Thermal Lens Microscope:  Subsingle-Molecule Determination
Manabu Tokeshi, Marika Uchida, Akihide Hibara, Tsuguo Sawada, Takehiko Kitamori
Analytical Chemistry, 73, 9, 2112, 2116, 29 Mar. 2001, [Peer-reviewed], [Lead author]
English, Scientific journal

On-Chip Integration of Neutral Ionophore-Based Ion Pair Extraction Reaction
Hideaki Hisamoto, Takayuki Horiuchi, Manabu Tokeshi, Akihide Hibara, Takehiko Kitamori
Analytical Chemistry, 73, 6, 1382, 1386, 10 Feb. 2001, [Peer-reviewed]
English, Scientific journal

Determination of Carcinoembryonic Antigen in Human Sera by Integrated Bead-Bed Immunoasay in a Microchip for Cancer Diagnosis
Kiichi Sato, Manabu Tokeshi, Hiroko Kimura, Takehiko Kitamori
Analytical Chemistry, 73, 6, 1213, 1218, 10 Feb. 2001, [Peer-reviewed]
English, Scientific journal

Acceleration of an enzymatic reaction in a microchip
Yuki Tanaka, Maxim N. Slyadnev, Kiichi Sato, Manabu Tokeshi, Haeng-Boo Kim, Takehiko Kitamori
Analytical Sciences, 17, 7, 809, 810, 2001, [Peer-reviewed]
English, Scientific journal

Integration of a wet analysis system on a glass chip: determination of Co(ii) as 2-nitroso-1-naphthol chelates by solvent extraction and thermal lens microscopy
Tomoko Minagawa, Manabu Tokeshi, Takehiko Kitamori
Lab on a Chip, 1, 1, 72, 72, 2001, [Peer-reviewed]
English, Scientific journal

Optimization of the optical-scheme design for photothermal-lens microscopy in microchips
MA Proskurnin, M Tokeshi, MN Slyadnev, T Kitamori
Analytical Sciences, 17, S454, S457, 2001, [Peer-reviewed]
English, Scientific journal

Infrared Absorption Characteristics of Large-Sized Spherical Aryl-Ether Dendrimers
Yuki Wakabayashi, Manabu Tokeshi, Akihide Hibara, Dong-Lin Jiang, Takuzo Aida, Takehiko Kitamori
Analytical Sciences, 16, 12, 1323, 1326, 17 Dec. 2000, [Peer-reviewed]
English, Scientific journal

Non-contact photothermal control of enzyme reactions on a microchip by using a compact diode laser
Yuki Tanaka, Maxim N Slyadnev, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori
Journal of Chromatography A, 894, 1-2, 45, 51, Oct. 2000, [Peer-reviewed]
English, Scientific journal

Integration of a microextraction system
Manabu Tokeshi, Tomoko Minagawa, Takehiko Kitamori
Journal of Chromatography A, 894, 1-2, 19, 23, Oct. 2000, [Peer-reviewed], [Lead author]
English, Scientific journal

Molecular Transport between Two Phases in a Microchannel
Kiyoshi Sato, Manabu Tokeshi, Tsuguo Sawada, Takehiko Kitamori
Analytical Sciences, 16, 5, 455, 456, 17 May 2000, [Peer-reviewed]
English, Scientific journal

Integration of an immunosorbent assay system: Analysis of secretory human immunoglobulin A on polystyrene beads in a microchip
Kiichi Sato, Manabu Tokeshi, Tamao Odake, Hiroko Kimura, Takeshi Ooi, Masayuki Nakao, Takehiko Kitamori
Analytical Chemistry, 72, 6, 1144, 1147, 15 Mar. 2000, [Peer-reviewed]
English, Scientific journal

Integration of a Microextraction System on a Glass Chip:  Ion-Pair Solvent Extraction of Fe(II) with 4,7-Diphenyl-1,10-phenanthrolinedisulfonic Acid and Tri-n-octylmethylammonium Chloride
Manabu Tokeshi, Tomoko Minagawa, Takehiko Kitamori
Analytical Chemistry, 72, 7, 1711, 1714, 26 Feb. 2000, [Peer-reviewed], [Lead author]
English, Scientific journal

Integration of Flow Injection Analysis and Zeptomole-Level Detection of the Fe(II)-o-Phenanthroline Complex
Kiyoshi Sato, Manabu Tokeshi, Takehiko Kitamori, Tsuguo Sawada
Analytical Sciences, 15, 7, 641, 645, 15 Jul. 1999, [Peer-reviewed]
English, Scientific journal

Sub-Zeptomole Detection in a Microfabricated Glass Channel by Thermal-Lens Microscopy
Kiyoshi Sato, Hiroaki Kawanishi, Manabu Tokeshi, Takehiko Kitamori, Tsuguo Sawada
Analytical Sciences, 15, 6, 525, 529, 23 Jun. 1999, [Peer-reviewed]
English, Scientific journal

Single- And countable-molecule detection of non-fluorescent molecules in liquid phase
Manabu Tokeshi, Marika Uchida, Kenji Uchiyama, Tsuguo Sawada, Takehiko Kitamori
Journal of Luminescence, 83-84, 261, 264, Elsevier, 1999, [Peer-reviewed]
English, Scientific journal

Long-term energy storage of dendrimers
Yuki Wakabayashi, Manabu Tokeshi, Dong-Ling Jiang, Takuzo Aida, Takehiko Kitamori
Journal of Luminescence, 83-84, 313, 315, Elsevier, 1999, [Peer-reviewed]
English, Scientific journal

Photoionization Threshold of Perylene on Water Surface as Measured by Synchrotron Radiation
Takanori Inoue, Shin-ya Sasaki, Manabu Tokeshi, Teiichiro Ogawa
Chemistry Letters, 27, 7, 609, 610, Oxford University Press (OUP), 01 Jul. 1998, [Peer-reviewed]
English, Scientific journal, Abstract

Photoionization efficiency of perylene on water surface was measured in the 165-260 nm region by synchrotron radiation. The photoionization threshold was determined to be 5.9 eV and agreed well with that determined in the laser two-photon ionization. This finding indicates that the water surface is more hydrophobic than its bulk.

Development of Tunable Ultraviolet Laser System Synchronizing Precisely with Synchrotron Radiation Pulses from UVSOR　　　　　　　　　　　　　　　
Masakazu Mizutani, Manabu Tokeshi, Atsunari Hirata, Koichiro Mitsuke
Journal of Synchrotron Radiation, 4, 6, 13, 1997, [Peer-reviewed]
English, Scientific journal

Doppler Profiles of the Balmer-α and -β Lines of the Excited Hydrogen Atoms (n = 3,4) Produced in Ar+-C2H6 Collisions
Manadu Tokeshi, Keiji Nakashima, Teiichiro Ogawa
Chemistry Letters, 25, 11, 943, 944, Oxford University Press (OUP), 01 Nov. 1996, [Peer-reviewed], [Lead author]
English, Scientific journal, Abstract

The Doppler profiles of the Balmer lines are very broad; their width decreases at lower ion energies. The translational energy distributions of H* calculated from the Doppler profile have two major components at about 10 eV and at about 100 eV for 2.5 keV of Ar+.

Dissociative excitation of aliphatic hydrocarbons (C2H2n: n = 1, 2, 3) by fast argon ion impact: Rovibrational distribution of CH(A2Δ)
Manabu Tokeshi, Keiji Nakashima, Teiichiro Ogawa
Chemical Physics, 206, 1-2, 237, 243, Elsevier BV, May 1996, [Peer-reviewed], [Lead author]
English, Scientific journal

Dissociation of aliphatic hydrocarbons by controlled electron impact: Vibrational and rotational energy distributions of the excited CH radicals
Manabu Tokeshi, Keiji Nakashima, Teiichiro Ogawa
Chemical Physics, 203, 2, 257, 266, Elsevier BV, Feb. 1996, [Peer-reviewed], [Lead author]
English, Scientific journal

Direct Collisional Scattering of the Excited Hydrogen Atom: Doppler Profile Analysis in the Fast Ar+ Ions Collisions with Acetylene and Ethylene　　　　　　　　　　　　　　　
Manabu Tokeshi, Keiji Nakashima, Teiichiro Ogawa
Chemical Physics Letters, 263, 783, 788, 1996, [Peer-reviewed], [Lead author]
English, Scientific journal

Rovibrational Distributions of CH(B 2.SIGMA.-) Produced in Electron-C2H2 Collisions
Kenji Furuya, Akihiro Matsuo, Manabu Tokeshi, Teiichiro Ogawa
The Journal of Physical Chemistry, 99, 38, 13772, 13777, American Chemical Society (ACS), Sep. 1995, [Peer-reviewed]
English, Scientific journal

Rovibrational Distributions of CH(A2Δ) Produced in e-C2H2 Collisions
Manabu Tokeshi, Keiji Nakashima, Teiichiro Ogawa
Chemistry Letters, 22, 6, 995, 996, Oxford University Press (OUP), 01 Jun. 1993, [Peer-reviewed], [Lead author]
English, Scientific journal, Abstract

Emission spectra of the CH(A2Δ-X2Π) transition produced in the electron impact dissociation of acethylene have been measured. The rovibrational distributions of CH(A2Δ) depend on the electron impact energy; the rotational temperature of CH(A2Δ, v′ = 0) varied from 3200 K at 17 eV to 2500 K at 100 eV, and the ratio of the vibrational populations (P(v′ = 1)/P(v′ = 0)) varied from 0.45 at 17 eV to 0.80 at 100 eV.

粒径制御型ハイブリッドエクソソーム作製法の開発
大山祥大, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

タンパク質結晶のサイズ分離デバイスの開発とX線結晶構造解析への応用
平山兼光, 真栄城正寿, 真栄城正寿, 真栄城正寿, 上野剛, 坂井直樹, 日比野光恵, 石田晃彦, 山本雅樹, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

深紫外発光ダイオードを光源とするポータブル吸光度検出液体クロマトグラフの開発
小山魁人, 石田晃彦, 日比野光恵, 真栄城正寿, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

タンパク質X線結晶構造解析の自動化のためのオールインワンデバイスの開発
一町田由貴, 真栄城正寿, 真栄城正寿, 真栄城正寿, 上野剛, 小西真昌, 坂井直樹, 日比野光恵, 石田晃彦, 山本雅樹, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

人工エクソソームのRNA送達における膜タンパク質の機能解明
荒谷響子, 丹羽彩由花, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

アプタマーを用いた非競合蛍光偏光分析法における検出感度の向上
千田駿亮, 植咲月, 福山真央, 粕谷素洋, 日比野光恵, 石田晃彦, 真栄城正寿, 火原彰秀, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

マイクロ流体デバイスを用いた核酸搭載ウイルス様粒子作製法の開発
杉浦魁星, 岡田悠斗, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

Development of a SARS-CoV-2-like particles preparation method using microfluidic devices and its application to nucleic acid delivery
杉浦魁星, 岡田悠斗, 真栄城正寿, 真栄城正寿, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム, 23, 1, 2024

細胞外小胞の単一粒子解析と炎症の早期検出
川口彰太, 阿尻大雅, 神谷由紀子, 真栄城正寿, 渡慶次学, 古賀大尚, 村上正晃, 安井隆雄, 安井隆雄, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 49th, 2024

マイクロ流体デバイスを用いた人工エクソソームの開発
丹羽彩由花, 大山祥大, 真栄城正寿, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

ポリカチオン/DNA複合体搭載脂質ナノ粒子による効率的な長鎖DNAトランスフェクション
宇野秀哉, 真栄城正寿, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 日比野光恵, 谷博文, 原島秀吉, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

カリウム・硝酸・リン酸イオンの同時定量のための紙分析デバイスの開発
才木陸朗, 石田晃彦, 日比野光恵, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

紙ベースの分析デバイスを用いた生物発光レポーターアッセイ
古坐あすか, 日比野光恵, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

LNP production method and process
真栄城正寿, 渡慶次学, Pharm Tech Japan, 39, 3, 2023

小型測定デバイスからなるATPの簡便かつ迅速な比色定量システム
菊知龍哉, 石田晃彦, 日比野光恵, 真栄城正寿, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 72nd, 2023

LEDベースのUV吸光度検出小型ユニットからなるパームトップ液体クロマトグラフ
小山魁人, 石田晃彦, 日比野光恵, 真栄城正寿, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 72nd, 2023

非競合蛍光偏光分析法の検出感度に影響する因子:トレーサーの蛍光位置および蛍光色素の寿命
千田駿亮, 植咲月, 福山真央, 粕谷素洋, 日比野光恵, 石田晃彦, 真栄城正寿, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

タンパク質のX線結晶構造解析の自動化に向けたマイクロ流体デバイスの開発
一町田由貴, 真栄城正寿, 真栄城正寿, 真栄城正寿, 上野剛, 小西真昌, 坂井直樹, 石田晃彦, 山本雅樹, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

マイクロ流体デバイスを用いたSARS-CoV-2様粒子作製法の開発と薬剤送達への応用
杉浦魁星, 岡田悠人, 真栄城正寿, 真栄城正寿, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

マイクロ流体デバイスを用いたハイブリッドエクソソームの作製
大山祥大, 真栄城正寿, 日比野光恵, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

Title Development of a portable liquid chromatography system with an electroosmotic pump and chip-based column
石田晃彦, 小山魁人, 日比野光恵, 真栄城正寿, 渡慶次学, キャピラリー電気泳動シンポジウム講演要旨集, 43rd (CD-ROM), 2023

ナノin situハイブリダイゼーション法によるエクソソームのデジタル解析
川口彰太, 安井隆雄, 安井隆雄, 安井隆雄, 嶋田泰佑, 神谷由紀子, 真栄城正寿, 渡慶次学, 古賀大尚, 村上正晃, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th, 2023

Improved Sensitivity of Bioluminescence Assay in a Paper-Based Analytical Device as a Platform
田中楓子, 大山祥大, 石田晃彦, 真栄城正寿, 渡慶次学, 藤原政司, 田島健次, 谷博文, セルロース学会年次大会講演要旨集, 30th, 2023

Effect of Lipid Nanoparticle Properties on the Therapeutic Effects against Acetaminophen Liver Injury
日比野光恵, 日比野光恵, 真栄城正寿, 渡慶次学, 原島秀吉, 山田勇磨, 日本薬剤学会年会講演要旨集(CD-ROM), 38th, 2023

難水溶性物質CoQ₁₀を搭載可能な脂質ナノ粒子設計の検討
杉原崚太, 日比野光恵, 真栄城正寿, 渡慶次学, 中村孝司, 原島秀吉, 山田勇磨, 生体膜と薬物の相互作用シンポジウム講演要旨集, 44th, 2023

Validation of mitochondrial-derived component supplementation strategy for the purpose of activating mitochondrial function
伊藤百, 山田勇磨, 山田勇磨, 真栄城正寿, 渡慶次学, 原島秀吉, 日本薬学会年会要旨集(Web), 143rd, 2023

Evaluation of an innovative mitochondrial genome-editing system using mitochondria-targeted nanoparticles in vitro
野呂田楓, 石塚宣, 廣瀬みさ, 真栄城正寿, 渡慶次学, 原島秀吉, 山田勇磨, 山田勇磨, 日本薬学会年会要旨集(Web), 143rd, 2023

マイクロ流体デバイスを用いた脂質・エクソソーム様ナノ粒子作製と核酸送達への応用
真栄城正寿, 真栄城正寿, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 47th, 2023

ブロックコポリマー/機能性ペプチド共集合化ナノ粒子の調製と経鼻投与による脳脊髄への核酸DDSキャリアとしての有用性評価
青木駿典, 金沢貴憲, 金沢貴憲, 飯岡真吾, 照喜名孝之, 真栄城正寿, 渡慶次学, 近藤啓, 日本DDS学会学術集会プログラム予稿集, 39th, 2023

特性の異なるコエンザイムQ₁₀搭載脂質ナノ粒子を用いたアセトアミノフェン肝障害に対する治療効果の検証
日比野光恵, 真栄城正寿, 渡慶次学, 原島秀吉, 山田勇磨, 日本DDS学会学術集会プログラム予稿集, 39th, 2023

CRISPR/Cas9システムを用いたmtDNAゲノム編集の検証
野呂田楓, 野呂田楓, 野呂田楓, 石塚宣, 廣瀬みさ, 真栄城正寿, 渡慶次学, 原島秀吉, 山田勇磨, 日本DDS学会学術集会プログラム予稿集, 39th, 2023

癌光治療用ミトコンドリア標的型ナノカプセルの殺細胞効果向上を目指した試み
長縄莉奈, 高野勇太, 高野勇太, 趙韓俊, 真栄城正寿, 渡慶次学, 原島秀吉, 山田勇磨, 日本DDS学会学術集会プログラム予稿集, 39th, 2023

セルロースナノファイバシートによる単一細胞外小胞解析
川口彰太, 安井隆雄, 安井隆雄, 安井隆雄, 嶋田泰佑, 神谷由紀子, 浅沼浩之, 真栄城正寿, 渡慶次学, 古賀大尚, 村上正晃, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 47th, 2023

Single extracellular vesicle analysis with drying shrinkage of cellulose nanofiber paper
川口彰太, 安井隆雄, 安井隆雄, 安井隆雄, 嶋田泰佑, 神谷由紀子, 浅沼浩之, 真栄城正寿, 渡慶次学, 古賀大尚, 村上正晃, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会春季年会講演予稿集(Web), 103rd, 2023

酵素と基質をセルロース媒体に固定化した電気化学ATP-乳酸センサー
九鬼静香, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

ルシフェラーゼ発現大腸菌の紙デバイス上での発現誘導
古坐あすか, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

抗SARS-CoV-2抗体およびSARS-CoV-2スパイクタンパク質断片の非競合蛍光偏光免疫分析法
高橋和希, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

薬剤送達ナノ粒子作製用ガラス製マイクロ流体システムの開発
岡田悠斗, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

タンパク質分析用ペーパー分析デバイスの開発を目的としたろ紙上でのホタルルシフェラーゼ活性の制御
野中康伸, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

タンパク質-リガンド複合体解析のためのマイクロ流体デバイスの開発
舟久保智瑛, 真栄城正寿, 上野剛, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

Preparation of various block copolymer nanoparticles by microfluidic device method and evaluation of their physical properties and brain delivery
吉田圭輝, 金沢貴憲, 飯岡真吾, 真野結奈, 照喜名孝之, 真栄城正寿, 渡慶次学, 近藤啓, 日本薬学会年会要旨集(Web), 142nd, 2022

ミトコンドリア標的型DDSを基盤とした癌光治療の検証
山田勇磨, 窪田文佳, 高野勇太, BIJU Vasudevanpillai, 真栄城正寿, 渡慶次学, 繁富香織, 宮武由甲子, 原島秀吉, 日本DDS学会学術集会プログラム予稿集, 38th, 2022

コエンザイムQ₁₀搭載ミトコンドリア標的型ナノカプセル注射剤の開発
日比野光恵, 山田勇磨, 真栄城正寿, 渡慶次学, 原島秀吉, 生体膜と薬物の相互作用シンポジウム講演要旨集, 43rd, 2022

ルシフェラーゼ発現大腸菌の紙デバイスへの固定化と発現誘導
古坐あすか, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 71st, 2022

エクソソームによるリキッドバイオプシーのための標準脂質ナノ粒子の開発
大山祥大, 丹羽彩由花, 真栄城正寿, 真栄城正寿, 真栄城正寿, 石田晃彦, 谷口正輝, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 71st, 2022

カリウムイオン定量のための距離ベース紙分析デバイスの開発
才木陸朗, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th, 2022

蛍光ナノダイヤモンド搭載セラノスティクス用脂質ナノ粒子の作製
杉浦魁星, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 45th, 2022

マイクロ流体デバイスを用いた人工エクソソーム作製法の開発
丹羽彩由花, 大山祥大, 真栄城正寿, 真栄城正寿, 石田晃彦, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th, 2022

タンパク質含有ハイドロゲルによるX線結晶構造解析法の開発
一町田由貴, 真栄城正寿, 真栄城正寿, 上野剛, 小西真昌, 坂井直樹, 石田晃彦, 谷博文, 山本雅樹, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 45th, 2022

マイクロ流体デバイスを用いた長鎖プラスミドDNA搭載ナノ粒子の作製
宇野秀哉, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th, 2022

セルロースナノファイバーの乾燥収縮を介した膜融合反応による単一EV統合解析
川口彰太, 安井隆雄, 安井隆雄, 安井隆雄, 嶋田泰佑, 神谷由紀子, 浅沼浩之, 真栄城正寿, 渡慶次学, 古賀大尚, 村上正晃, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th, 2022

Construction of preparation method using microfluidic device and validation of therapeutic effect for mitochondrial targeted coenzyme Q₁₀ encapsulated nanocapsule injectable drug
日比野光恵, 山田勇磨, 真栄城正寿, 渡慶次学, 原島秀吉, 日本薬学会年会要旨集(Web), 142nd, 2022

Validation of mitochondrial component supplementation strategy to activate mitochondrial function
伊藤百, 山田勇磨, 山田勇磨, 日比野光恵, 佐々木大輔, 真栄城正寿, 渡慶次学, 渡慶次学, 太田善浩, 原島秀吉, 原島秀吉, 日本薬学会年会要旨集(Web), 142nd, 2022

DEVELOPMENT OF AN ELECTROCHEMICAL SENSOR FOR DIAGNOSING SEVERITY USING BLOOD LACTATE / ATP RATIO AS A BIOMARKER
九鬼静香, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, Chemical Sensors, 37, Supplement B, 2021

ポータブル蛍光偏光測定装置を用いたヒト血清中の抗SARS-CoV-2抗体の検出
高橋和希, 西山慶音, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 分析化学討論会講演要旨集(Web), 81st, 2021

エタノール混合によるベシクル構造転移:マルチラメラ形成機構
柴田果奈, 真栄城正寿, 渡慶次学, 篠田渉, 篠田渉, 分子シミュレーション討論会講演要旨集, 35th (CD-ROM), 2021

Development Of An Electrochemical Sensor For Diagnosing Severity Using Blood Lactate / ATP Ratio As A Biomarker
九鬼静香, 石田晃彦, 真栄城正寿, 谷博文, 渡慶次学, 電気化学秋季大会講演要旨集(CD-ROM), 2021, 2021

ミトコンドリア標的型光線力学的療法を可能とするナノキャリアの構築及び機能評価
窪田文佳, 山田勇磨, SATRIALDI, 高野勇太, 真栄城正寿, 渡慶次学, 原島秀吉, 生体膜と薬物の相互作用シンポジウム講演要旨集, 42nd (CD-ROM), 2021

マイクロ流体デバイスを用いたプラスミドDNA搭載脂質ナノ粒子の作製
宇野秀哉, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 43rd, 2021

蛍光偏光免疫分析法によるオカダ酸の検出
千田駿亮, 高橋和希, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, ZHERDEV Anatoly V., EREMIN Sergei A., EREMIN Sergei A., 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 44th, 2021

Affibodyを用いた非競合蛍光偏光免疫分析法によるEGFRの検出
高橋和希, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 44th, 2021

Trans MIT system
山田勇磨, 山田勇磨, 日比野光恵, 伊藤百, 荒井愛永, 佐々木大輔, 真栄城正寿, 渡慶次学, 渡慶次学, 太田善浩, 原島秀吉, 原島秀吉, 日本薬剤学会年会講演要旨集(CD-ROM), 36th, 2021

Properties of lipid nanoparticles affecting their lymph node transitivity and distribution
中村孝司, 河合美典, 佐藤悠介, 真栄城正寿, 渡慶次学, 原島秀吉, 日本薬剤学会年会講演要旨集(CD-ROM), 36th, 2021

Fundamental study on preparation of polymer nanoparticles by microfluidic device
飯岡真吾, 日比野光恵, 山田勇磨, 真栄城正寿, 渡慶次学, 照喜名孝之, 金沢貴憲, 近藤啓, 日本薬学会年会要旨集(Web), 141st, 2021

蛍光偏光免疫測定法によるヒト血清中の抗SARS-CoV-2抗体の検出
高橋和希, 西山慶音, 福山真央, 粕谷素洋, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, キャピラリー電気泳動シンポジウム講演要旨集, 40th (CD-ROM), 2020

ナノ構造体を用いた脂質ナノ粒子のサイズ分離
清水一樹, 真栄城正寿, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

ペーパーデバイスによる懸濁試料の簡易・迅速分析のための前処理ユニットの開発
前田陵我, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

血中リチウムイオン測定のための全自動分析システムの開発
小松雄士, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

ポータブルHPLCのためのLEDおよびフォトダイオードを用いた小型吸光検出モジュールの開発
西村卓馬, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

ルシフェラーゼの活性制御に基づくオンチップ生物発光アッセイの開発
野中康伸, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

常温X線結晶構造解析のためのマイクロデバイスの開発
舟久保智瑛, 真栄城正寿, 真栄城正寿, 伊藤翔, 伊藤翔, 上野剛, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

生物発光分析のためのペーパーデバイスの開発とキナーゼ分析への応用
高田一生, 渡慶次学, 谷博文, 石田晃彦, 真栄城正寿, 分析化学討論会講演要旨集(Web), 80th, 2020

高感度免疫分析デバイスの開発とH5型鳥インフルエンザウイルス検出への応用
西山慶音, RAMOS Kenia Chavez, 真栄城正寿, 石田晃彦, 谷博文, 笠間敏博, 馬場嘉信, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

乳酸/ATPの血中濃度比による重症度診断のための電気化学酵素センサーの開発
九鬼静香, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集(Web), 80th, 2020

Development of Micro Device for Simple and Rapid Measurement of Progesterone Using Paper Substrates.
小松雄士, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 日本化学会春季年会講演予稿集(CD-ROM), 100th, 2020

紙ベースイムノアッセイのための抗体の簡便な固定化法
前田陵我, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

ナノ構造体搭載デバイスによる脂質ナノ粒子のサイズ分離
清水一樹, 真栄城正寿, 真栄城正寿, 石田晃彦, 谷博文, 西井準治, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

Size and charge effect of lipid nanoparticles on transitivity to lymph node and distribution in lymph node
中村孝司, 河合美典, 佐藤悠介, 真栄城正寿, 渡慶次学, 原島秀吉, 日本薬学会年会要旨集(CD-ROM), 140th (Web), 2020

CRISPR/Casタンパク質搭載脂質ナノ粒子の開発
佐藤悠介, 鈴木裕一, 小沼はるの, 佐藤里咲, 真栄城正寿, 渡慶次学, 原島秀吉, 日本DDS学会学術集会プログラム予稿集, 36th, 2020

コエンザイムQ10封入ミトコンドリア標的型DDSの開発およびアセトアミノフェン肝障害モデルへの治療効果の検証
日比野光恵, 山田勇磨, 真栄城正寿, 渡慶次学, 石塚洋一, 原島秀吉, 日本DDS学会学術集会プログラム予稿集, 36th, 2020

ガラス製マイクロ流体デバイスを用いたsiRNA搭載脂質ナノ粒子の作製と大量生産用集積化デバイスの開発
真栄城正寿, 真栄城正寿, 岡田悠斗, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 日本DDS学会学術集会プログラム予稿集, 36th, 2020

DDSナノ粒子作製用ガラス製マイクロ流体デバイスの開発
岡田悠斗, 真栄城正寿, 真栄城正寿, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

マイクロデバイスを用いたタンパク質結晶の室温複合体構造解析
竹田怜央, 真栄城正寿, 真栄城正寿, 伊藤翔, 伊藤翔, 上野剛, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 42nd (Web), 2020

UNDERSTANDING THE LIPID NANOPARTICLES STRUCTURE DYNAMICS USING A TIME-RESOLVED SAXS MEASUREMENT
Masatoshi Maeki, Niko Kimura, Kazuki Shimizu, Kento Yonezawa, Nobutaka Shimizu, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi, Proc. Micro TAS 2019, 1478, 1479, Oct. 2019, [Peer-reviewed]
English

PORTABLE FLUORESCENCE POLARIZATION ANALYZER FOR ON-SITE MULTISAMPLE IMMUNOASSAY
Ayano Nakamura, Osamu Wakao, Ken Satou, Mitsutoshi Aoyagi, Kazuhiko Nishimura, Chikaaki Mizokuchi, Ken Sumiyoshi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi, Proc. Micro TAS 2019, 1393, 1394, Oct. 2019, [Peer-reviewed]
English

DEVELOPMENT OF A THREE-DIMENSIONAL MICROMIXER DEVICE FOR PRODUCTION OF VARIOUS LIPID-BASED NUCLEIC ACID NANOCARRIERS
Niko Kimura, Masatoshi Maeki, Yusuke Sato, Kosuke Sasaki, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi, Proc. Micro TAS 2019, 368, 369, Oct. 2019, [Peer-reviewed]
English

HIGH-THROUGHPUT X-RAY CRYSTALLOGRAPHY BASED ON THE PROTEIN CRYASTAL ARRAY
Reo Takeda, Masatoshi Maeki, Sho Ito, Go Ueno, Kunio Hirata, Akihiko Ishida, Hirofumi Tani, Masaki Yamamoto, Manabu Tokeshi, Proc. Micro TAS 2019, 191, 192, Oct. 2019, [Peer-reviewed]
English

FLUORESCENCE SIGNAL AMPLIFICATION FOR SENSITIVE ENZYME IMMUNOASSAY UTILIZING AN IMMUNO-WALL
Keine Nishiyama, Toshihiro Kasama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi, Proc. Micro TAS 2019, 689, 690, Oct. 2019, [Peer-reviewed]
English

実験計画法によるmRNA送達キャリアの効率的な最適化
橋場月, 佐藤悠介, 真栄城正寿, 渡慶次学, 原島秀吉, 日本核酸医薬学会年会講演要旨集, 5th, 2019

メタン代謝酵素を用いたメタン特異的な分析手法の開発
清田雄平, 真栄城正寿, 渡慶次学, 宮崎真佐也, 分析化学討論会講演要旨集, 79th, 2019

フローデバイスを用いたフルオレセイン振動化学発光
池田晋吾, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集, 79th, 2019

ゲノム編集タンパク質搭載脂質ナノ粒子の開発
佐藤悠介, 鈴木裕一, 佐藤里咲, 真栄城正寿, 渡慶次学, 原島秀吉, 日本核酸医薬学会年会講演要旨集, 5th, 2019

マイクロデバイスを用いたタンパク質-リガンド複合体構造解析
真栄城正寿, 真栄城正寿, 竹田怜央, 伊藤翔, 伊藤翔, 上野剛, 平田邦生, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 分析化学討論会講演要旨集, 79th, 2019

電気浸透流ポンプとオンチップカラムに基づく可搬型イオンクロマトグラフの開発
石田晃彦, 西村卓馬, 真栄城正寿, 谷博文, 渡慶次学, イオンクロマトグラフィー討論会講演要旨集, 36th, 2019

Microfluidic Device: As a Tool of Pharmaceutical Research and Development
西山慶音, 木村笑, 真栄城正寿, 渡慶次学, 薬剤学(Web), 79, 3, 2019

核酸搭載脂質ナノ粒子の動的構造解析
真栄城正寿, 真栄城正寿, 木村笑, 清水一樹, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 40th, 2019

核酸ナノキャリア製造のための新規バッフルデバイスの開発
木村笑, 真栄城正寿, 真栄城正寿, 石田明彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 40th, 2019

蛍光偏光免疫分析法によるH5亜型鳥インフルエンザウイルスの検出
西山慶音, 武田洋平, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 小川晴子, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 40th, 2019

マイクロデバイスを用いたX線結晶構造解析の高速化とリガンドスクリーニングへの応用
竹田怜央, 真栄城正寿, 真栄城正寿, 伊藤翔, 伊藤翔, 上野剛, 平田邦夫, 石田晃彦, 谷博文, 山本雅貴, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 39th, 2019

脂質ナノ粒子形成後の二次希釈操作が粒子サイズへ与える影響の解明
木村笑, 真栄城正寿, 岡部奈々, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 39th, 2019

マイクロ流路デバイスによるメタン分析のための新規メタン特異的検出法の開発
清田雄平, 真栄城正寿, 渡慶次学, 宮崎真佐也, 宮崎真佐也, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 39th, 2019

小角X線溶液散乱法による脂質ナノ粒子の動的構造解析法の開発
真栄城正寿, 木村笑, 石田晃彦, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 68th, 2019

電気浸透流ポンプとオンチップカラムに基づくオールインワンコンパクトLCシステムの開発
石田晃彦, 西村卓馬, 真栄城正寿, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集(Web), 68th, 2019

Bioanalysis: Applications of Microfluidic Systems in Biology and Medicine　　　　　　　　　　　　　　　
Tokeshi Manabu, Daisuke Onoshima, Hiroshi Yukawa, Yoshinobu Baba, Bioanalysis: Applications of Microfluidic Systems in Biology and Medicine, 7, 275, 300, 2019
English, Others

Microfluidic stepwise and rapid ethanol dilution for precise size control of lipid nanoparticles　　　　　　　　　　　　　　　
Niko Kimura, Masatoshi Maeki, Nana Okabe, Yusuke Sato, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi, Proc. Micro TAS 2018, 1404, 1405, Nov. 2018, [Peer-reviewed]
English

MICROSCOPIC REAL-TIME MEASUREMENT OF PROTEIN CRYSTAL GROWTH IN MICROFLUIDIC DEVICES
Masatoshi Maeki, Shohei Yamazaki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi, Proc. Micro TAS 2018, 4, 2192, 2193, Nov. 2018, [Peer-reviewed]
English

ナノピラーデバイスを用いたDNAのサイズ分離と無標識検出　　　　　　　　　　　　　　　
阿尻 大雅, 安井 隆雄, 笠 晴也, 真栄城 正寿, 石田 晃彦, 谷 博文, 西井 準治, 馬場 嘉信, 渡慶次 学, 日本分析化学会講演要旨集, 67年会, 125, 125, Aug. 2018
(公社)日本分析化学会, Japanese

電気化学検出液体クロマトグラフィーチップの開発とその尿中シュウ酸分析への応用
藤井大地, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

マイクロ流体デバイスにおけるルミノール化学発光の増感効果
菊地優仁, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

マイクロデバイスを用いた小麦中デオキシニバレノールの多サンプル同時蛍光偏光免疫分析
中村彩乃, 若尾摂, 佐藤憲, 住吉研, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 37th, 2018

微小空間内におけるリゾチームの結晶化制御および晶析挙動解析
山崎翔平, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

Simultaneous Multiplex Determination of Deoxynivalenol in Wheat By Fluorescence Polarization Immunoassay Using Microdevice
中村彩乃, 若尾摂, 佐藤憲, 住吉研, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム, 17, 2, 2018

オンチップ液体クロマトグラフィーのためのウォールジェット式電気化学検出器の開発
干川晃生, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集, 67th, 2018

マイクロデバイスを用いた小型蛍光偏光測定装置の開発とその応用
中村彩乃, 若尾摂, 佐藤憲, 溝口親明, 住吉研, 黒澤文夫, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

ペーパーデバイスによるPOCTのための高感度競合イムノアッセイの実現
前田陵我, 小松雄士, 佐藤優樹, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

ハイスループット蛍光偏光イムノアッセイデバイスの開発
若尾摂, 中村彩乃, 佐藤憲, 溝口親明, 住吉研, 黒澤文夫, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

シクロデキストリンと両親媒性物質を用いたホタルルシフェラーゼの活性制御と生物発光分析法への応用
工藤裕貴, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 分析化学討論会講演要旨集, 78th, 2018

固定化酵素の再活性化法の開発とマイクロリアクターへの応用
清田雄平, 山口浩, 真栄城正寿, 渡慶次学, 宮崎真佐也, 宮崎真佐也, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

酵素免疫測定法を利用した高感度イムノウォールデバイスの開発
西山慶音, 笠間敏博, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

ペーパーデバイスによるリチウムイオン濃度測定
小松雄士, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

微小空間におけるタンパク質結晶成長のリアルタイム計測
竹田怜央, 山崎翔平, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 38th, 2018

イムノウォールデバイスの開発と遺伝子変異たんぱく質の検出
中股征哉, 笠間敏博, 笠間敏博, 長谷哲成, 與語直之, 與語直之, 小沢直也, 真栄城正寿, 石田晃彦, 佐藤光夫, 加地範匡, 谷博文, 長谷川好規, 馬場嘉信, 馬場嘉信, 渡慶次学, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

iLiNPデバイスによるpH応答性カチオン性脂質ナノ粒子の粒径制御
木村笑, 真栄城正寿, 岡部奈々, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 37th, 2018

新規製造現場での基盤技術 脂質ナノ粒子作製用マイクロ流体デバイス
木村笑, 真栄城正寿, 渡慶次学, ケミカルエンジニヤリング, 63, 6, 2018

Microfluidic immunoassay devices as next-generation cancer and medical diagnostics platform
Toshihiro Kasama, Yoshinobu Baba, Manabu Tokeshi, Next Generation Point-of-care Biomedical Sensors Technologies for Cancer Diagnosis, 305, 322, 30 Dec. 2017

A Small-Sized Lipid Nanoparticles Production Method Using Microfluidic Devices with Baffle Structures
N. Kimura, M. Maeki, Y. Sato, A. Ishida, H. Tani, H. Harashima, M. Tokeshi, Proc. Micro TAS 2017, 965, 966, Oct. 2017
English

High-Throughput Fluorescence Polarization Measurement System Towards Molecular Interaction Analysis
O. Wakao, M. Maeki, A. Ishida, H. Tani, A. Hibara, M. Tokeshi, Proc. Micro TAS 2017, 557, 558, Oct. 2017, [Peer-reviewed]
English

A Microfluidic-Based Technique for Creating Amyloid Nanostructures and Its Application to Enzyme Reaction
M. Maeki, S. Sato, A. Ishida, H. Tani, M. Tokeshi, Proc. Micro TAS 2017, 1279, 1280, Oct. 2017, [Peer-reviewed]
English

分子間相互作用測定のための多サンプル同時蛍光偏光測定システムの開発
若尾摂, 住吉研, 溝口親明, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, センサ・マイクロマシンと応用システムシンポジウム(CD-ROM), 34th, 2017

小型蛍光偏光イムノアッセイ装置の開発
若尾摂, 住吉研, 溝口親明, 真栄城正寿, 石田晃彦, 谷博文, 重村幸治, 火原彰秀, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

微小空間によるタンパク質結晶化制御と分光法を用いた晶析挙動解析
山崎翔平, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

マイクロデバイスを用いたアミロイド構造体作製法の開発と酵素反応への応用
真栄城正寿, 佐藤紗耶, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

高効率免疫分析を可能とするイムノウォールデバイスの開発
中股征哉, 笠間敏博, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 36th, 2017

DNA分離のためのナノピラーデバイス作製の簡便化とその応用
阿尻大雅, 安井隆雄, 安井隆雄, 笠晴也, 真栄城正寿, 石田晃彦, 谷博文, 西井準治, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, キャピラリー電気泳動シンポジウム講演要旨集, 37th, 2017

バッフル構造を配置した流路デザインによる粒径の精密制御
木村笑, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 35th, 2017

マイクロデバイスを用いた脂質ナノ粒子作製と生体内動態挙動
真栄城正寿, 木村笑, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学工学会秋季大会研究発表講演要旨集(CD-ROM), 49th, 2017

Lipid nanoparticle-size control by microchannel with baffle structures
木村笑, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学とマイクロ・ナノシステム, 16, 2, 2017

Nanopillars, Nanowires and Nanoballs for DNA and Protein Analysis
Noritada Kaji, Takao Yasui, Manabu Tokeshi, Yoshinobu Baba, RSC Nanoscience and Nanotechnology, 2017-January, 41, 76, 98, 2017

Development of a New Technique for Washing Steps in Multistep Assays Using Microfluidic Paper-based Analytical Devices
Saeed Mohammadi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi, Proceedings of MicroTAS 2016, 970, 971, Oct. 2016, [Peer-reviewed]
English, Introduction international proceedings

Fluorescence Polarization-based Multiple Samples Detection Using Microchamber Array Towards High-throughput Molecular Interaction Analysis
Osamu Wakao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi, Proceedings of MicroTAS 2016, 1400, 1401, Oct. 2016, [Peer-reviewed]
English, Introduction international proceedings

Micro/Nano Devices for Chemical Analysis
Manabu Tokeshi, Kiichi Sato, MICROMACHINES, 7, 9, Sep. 2016
English, Others

マイクロ分析チップの新たな可能性　　　　　　　　　　　　　　　
渡慶次学, 真栄城正寿, JPCA NEWS, 576, 8, 13, Sep. 2016, [Invited]
Japanese, Introduction commerce magazine

がん診断のためのエクソソーム分析法の開発　　　　　　　　　　　　　　　
阿尻 大雅, 安井 隆雄, 真栄城 正寿, 石田 晃彦, 谷 博文, 馬場 嘉信, 渡慶次 学, 日本分析化学会講演要旨集, 65年会, 165, 165, Aug. 2016
(公社)日本分析化学会, Japanese

Formation of Lipid Nanoparticles Using Microfluidic Devices and its Application for DDS
真栄城正寿, 佐藤悠介, 原島秀吉, 渡慶次学, 機能材料, 36, 7, 15, 21, Jul. 2016, [Invited]
シーエムシー出版, Japanese, Introduction commerce magazine

分子間相互作用測定のための蛍光偏光イメージングシステムの開発
若尾摂, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, 分析化学討論会講演要旨集, 76th, 2016

マイクロデバイスを用いたクロラムフェニコールの蛍光偏光免疫測定
青木琴, 若尾摂, 真栄城正寿, 石田晃彦, 谷博文, EREMIN Sergei, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 33rd, 2016

蛍光偏光イメージングによる複数サンプルの同時イムノアッセイ
若尾摂, 真栄城正寿, 石田晃彦, 谷博文, 火原彰秀, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

ATP測定のための電気化学酵素センサーの開発
西山慶音, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

マイクロチップ電気化学検出LCデバイスの生体試料分析への応用
藤井大地, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 日本分析化学会年会講演要旨集, 65th, 2016

ルシフェラーゼ内封リポソームを用いたオンチップイムノアッセイ
中谷友祐, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

マイクロデバイスによるタンパク質の立体構造解析
真栄城正寿, 真栄城正寿, 山崎翔平, 杉島正一, 渡邉啓一, 石田晃彦, 谷博文, 宮崎真佐也, 渡慶次学, 分析化学討論会講演要旨集, 76th, 2016

PDMS製チップホルダーを用いたペーパーマイクロ分析チップの開発
MOHAMMADI Saeed, BUSA Lori Shayne Alamo, 真栄城正寿, 石田晃彦, 谷博文, 渡慶次学, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 34th, 2016

脳腫瘍の完全切除を目指した術中遺伝子変異診断
笠間敏博, 笠間敏博, 笠間敏博, 山道茜, 山道茜, 大岡史治, 加藤幸成, 加藤彰, 平野雅規, LUSHUN Chalise, 栗本路弘, 近藤五郎, 青木恒介, 鈴木啓道, 本村和也, 加地範匡, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 渡慶次学, 松原年生, 鈴木秀鎌, 若林俊彦, 夏目敦至, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 34th, 2016

新規迅速解析デバイスを用いたグリオーマIDH1遺伝子変異の術中診断とその応用
山道茜, 山道茜, 大岡史治, 笠間敏博, 笠間敏博, 鈴木啓道, 青木恒介, 本村和也, 平野雅規, RANJIT Melissa, CHALISE Lushun, 栗本路弘, 近藤五郎, 加地範匡, 渡慶次学, 松原年生, 鈴木秀謙, 馬場嘉信, 加藤幸成, 木村晋也, 若林俊彦, 夏目敦至, 日本脳腫瘍学会プログラム・抄録集, 34th, 2016

がん診断のためのエクソソームの無標識検出
阿尻大雅, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 真栄城正寿, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, 2016

Immuno-wall lab-on-a-chip protein analysis devices for high precision surgery of gliomas
T. Kasama, A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, Y. Baba, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 651, 652, 2016, [Peer-reviewed]
English, Report scientific journal

Effects of the grooved structures and the ethanol concentration on the small-sized lipid nanoparticles formation
Y. Fujishima, M. Maeki, Y. Sato, T. Yasui, A. Ishida, H. Tani, Y. Baba, H. Harashima, M. Tokeshi, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 1412, 1413, 2016, [Peer-reviewed]
English, Introduction international proceedings

液晶ディスプレイを利用した蛍光偏光イメージング
若尾摂, 真栄城正寿, 火原彰秀, 渡慶次学, ケミカルエンジニアリング, 6, 1, 12, 17, Jan. 2016, [Invited]
化学工業社, Japanese, Introduction commerce magazine

マイクロ化学チップの最新動向：紙チップとタンパク質結晶構造解析チップ　　　　　　　　　　　　　　　
真栄城正寿, 渡慶次学, クリーン・テクノロジー, 25, 11, 1, 4, Nov. 2015, [Invited]
Japanese, Introduction commerce magazine

Fluorescence Polarization Imaging for Multisample Immunoassay
Osamu Wakao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi, Proceedings of MicroTAS 2015, 1816, 1818, Oct. 2015, [Peer-reviewed]
English, Introduction international proceedings

W221004 Medical Applications Using Microfluidic Devices
TOKESHI Manabu, Mechanical Engineering Congress, Japan, 2015, "W221004, 1"-"W221004-4", 13 Sep. 2015
Microfluidic devices have great potential for medical and life science applications. Recent progresses in microfluidic devices have enabled precise analysis of a small amount of proteins and drugs in blood or urine, and preparation of highly functional nanoparticles. We have developed that several immunoassay systems using the microfluidic devices for rapid diagnosis of biomakers, and have fabricated a novel non-viral gene delivery vector, lipid nanoparticles using the microfluidic devices. In this presentation, I will present about several topics: on-chip immunoassay using hydrogel pillars, fabrication of functional nanoparticles for drug delivery system, and so forth. Moreover, future challenges and potentials of microfluidic devices for biotechnology, medicine, and clinical diagnostics will be also discussed, The Japan Society of Mechanical Engineers, Japanese

Development of Microfluidic Devices for Rapid, Low-Cost Detection of EGFR Mutations in Cytological Samples from Patients with Lung Cancer
Tetsunari Hase, Toshihiro Kasama, Nanako Nishiwaki, Naoyuki Yogo, Mitsuo Sato, Noritada Kaji, Masashi Kondo, Manabu Tokeshi, Yoshinobu Baba, Yoshinori Hasegawa, JOURNAL OF THORACIC ONCOLOGY, 10, 9, S585, S585, Sep. 2015, [Peer-reviewed]
English, Summary international conference

紙を利用した分析・診断チップ　　　　　　　　　　　　　　　
真栄城正寿, 渡慶次学, 紙パルプ技術タイムス, 58, 37, 42, Aug. 2015, [Invited]
Japanese, Introduction commerce magazine

脂質ナノ粒子の粒径制御のためのマイクロ流体デバイス設計
真栄城正寿, 藤島由佳, 佐藤悠介, 石田晃彦, 谷博文, 原島秀吉, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 32nd, 2015

バイオミメティック・ケミストリーによる単一細胞からのRNA抽出法の創成
岡本行広, 大川智生, 小野島大介, 湯川博, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 95th, 1, 2015

バイオミメティック・ケミストリーによる単一細胞からのRNA抽出法の創成
岡本行広, 大川智生, 小野島大介, 湯川博, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 95th, 3, 2015

バイオミメティックな手法によるマイクロチップ表面の改質および分離への応用
岡本行広, 渡慶次学, 馬場嘉信, 分析化学討論会講演要旨集, 75th, 2015

肺癌体細胞遺伝子変異の免疫学的診断デバイスの開発
笠間敏博, 笠間敏博, 長谷哲成, 石川広弥, 石川広弥, 與語直之, 近藤征史, 加地範匡, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 渡慶次学, 長谷川好規, 長谷川好規, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 32nd, 2015

Immuno-wallマイクロ免疫診断チップによるIDH1遺伝子変異の迅速診断
山道茜, 山道茜, 大岡史治, 笠間敏博, 加藤幸成, 栗本路弘, 近藤五郎, 青木恒介, 鈴木啓道, 本村和也, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 鈴木秀謙, 若林俊彦, 夏目敦至, 日本脳腫瘍学会プログラム・抄録集, 33rd, 2015

Immuno-wall lab-on-chip companion diagnostic devices for rapid and low-cost detection of mutant epidermal growth factor receptors (EGFR) from cytological samples in lung cancer patients
T. Kasama, T. Hase, N. Nishiwaki, N. Yogo, M. Sato, M. Kondo, N. Kaji, M. Tokeshi, Y. Hasegawa, Y. Baba, MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 925, 927, 2015, [Peer-reviewed]
English, Report scientific journal

Label-free detection of extracellular vesicles for cancer diagnosis
Taiga Ajiri, Takao Yasui, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi, MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1789, 1791, 2015, [Peer-reviewed]
English, Report scientific journal

Microfluidic approach for production of lipid nanoparticles-based nano medicine
M. Maeki, T. Saito, Y. Node, Y. Sato, T. Yasui, N. Kaji, A. Ishida, H. Tani, Y. Baba, H. Harashima, M. Tokeshi, MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 838, 840, 2015, [Peer-reviewed]
English, Introduction international proceedings

2014年ノーベル化学賞 超解像蛍光顕微鏡の開発
加地 範匡, 渡慶次 学, 馬場 嘉信, 現代化学 = Chemistry today, 525, 31, 34, Dec. 2014
東京化学同人, Japanese

超解像蛍光顕微鏡の開発　　　　　　　　　　　　　　　
加地範匡, 渡慶次学, 馬場嘉信, 現代化学, 12, 31-34, 01 Dec. 2014
Japanese, Report scientific journal

LCD-CCD Synchronization Detection for Fluorescece Polarization Immunoassay　　　　　　　　　　　　　　　
Osamu Wakao, Yusaku Fujii, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi, Proceedings of the Micro TAS 2014 Symposium, 2271, 2273, Oct. 2014, [Peer-reviewed]
English, Introduction international proceedings

Crystal Habit Modification of Protein by Using Microfluidic Chip
Masatoshi Maeki, Ashtamurthy P. Pawate, Keiichi Watanabe, Manabu Tokeshi, Paul J. Kenis, Masaya Miyazaki, Proceedings of the Micro TAS 2014 Symposium, 1083, 1085, Oct. 2014, [Peer-reviewed]
English, Introduction international proceedings

細胞表層改質による単一細胞抽出法の創成　　　　　　　　　　　　　　　
岡本 行広, 大川 智生, 小野島 大介, 湯川 博, 渡慶次 学, 馬場 嘉信, 日本分析化学会講演要旨集, 63年会, 37, 37, Sep. 2014
(公社)日本分析化学会, Japanese

マイクロリアクタを用いたカリックスアレーン誘導体による希少金属分離プロセスの最適化
RAO Sathuluri. R., 真栄城正寿, 真栄城正寿, KIM Jee Young, 上田祐生, 大渡啓介, 渡慶次学, 宮崎真佐也, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

マイクロ流体デバイスによるタンパク質の結晶化制御技術
真栄城正寿, 真栄城正寿, PAWATE Ashtamurthy S., 渡邉啓一, 渡慶次学, KENIS Paul J. A., 宮崎真佐也, 日本結晶学会年会講演要旨集, 2014, 2014

マイクロ流路を用いたタンパク質結晶の晶癖制御
真栄城正寿, 真栄城正寿, 真栄城正寿, PAWATE Ashtamurthy, 渡邉啓一, 渡慶次学, KENIS Paul J. A., 宮崎真佐也, 宮崎真佐也, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 29th, 2014

簡便な稀少細胞同定・回収方法の創成
岡本行広, 湯川博, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 94th, 1, 2014

無動力駆動抽出法の創成
岡本行広, 湯川博, 渡慶次学, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 74th, 2014

無動力抽出法の創成
岡本行広, 湯川博, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 94th, 2, 2014

ナノバイオデバイスを用いたmicro-RNAの高速抽出
WU Qiong, WU Qiong, 安井隆雄, 安井隆雄, SAKON Rahong, 長島一樹, 柳田剛, 金井真樹, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 川合知二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 94th, 2, 2014

ナノ構造体を用いた無標識DNAの高感度検出
阿尻大雅, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 29th, 2014

治療標的としての上皮成長因子受容体遺伝子変異の特異的検出
笠間敏博, 笠間敏博, 長谷哲成, 近藤征史, 加地範匡, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 渡慶次学, 長谷川好規, 長谷川好規, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

マイクロ流路内におけるリポソーム形成メカニズムの考察
齋藤竜亮, 真栄城正寿, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

ナノ構造体の光回折を利用した高感度無標識検出法の開発
阿尻大雅, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

イムノウォールデバイスによる疾病マーカーのマルチプレックスアッセイ
西脇奈菜子, 笠間敏博, 石田晃彦, 谷博文, 馬場嘉信, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 30th, 2014

オンチップ制限酵素反応における遭遇複合体形成過程の1分子解析
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 94th, 2, 2014

小型・低コストな疾病マーカー定量分析装置の開発
笠間敏博, 笠間敏博, 長谷川寛, 近藤治靖, 小澤勉, 加地範匡, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 94th, 2, 2014

迅速・簡便疾患診断のためのイムノピラーデバイスの形状最適化
笠間敏博, 笠間敏博, 長谷川寛, 長谷川寛, 近藤治靖, 近藤治靖, 小澤勉, 小澤勉, 加地範匡, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 29th, 2014

Development of Label-Free Detection of Biomaterials Using Nanostructures
渡慶次 学, 旭硝子財団助成研究成果報告, 1, 6, 2014
旭硝子財団, Japanese

On-Chip Analysis of Intermittent Molecular Encounters in Nuclease Digestion of Specific DNA Sequence
Daisuke Onoshima, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, BIOPHYSICAL JOURNAL, 106, 2, 699A, 699A, Jan. 2014
English, Summary international conference

Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay
Toshihiro Kasama, Yutaka Hasegawa, Haruyasu Kondo, Tsutomu Ozawa, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 935, 937, 2014, [Peer-reviewed]
English, Introduction international proceedings

A millisecond micro-RNA extraction technique for nanopore-based nucleic acid sequencing
Qiong Wu, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 233, 235, 2014, [Peer-reviewed]
English, Introduction international proceedings

量子スイッチング効果による脳腫瘍細胞の高感度検出手法の構築
湯川 博, 塚本 涼子, 岡本 行広, 水野 正明, 加地 範匡, 渡慶次 学, 馬場 嘉信, Organ Biology, 20, 3, 105, 105, Oct. 2013
(一社)日本臓器保存生物医学会, Japanese

ナノワイヤデバイスを用いた細胞破砕　　　　　　　　　　　　　　　
安井 隆雄, 伊藤 聡, 柳田 剛, 加地 範匡, Yong He, Rahong Sakon, 金井 真樹, 長島 一樹, 渡慶次 学, 川合 知二, 馬場 嘉信, 日本分析化学会講演要旨集, 62年会, 145, 145, Aug. 2013
(公社)日本分析化学会, Japanese

Application of IgY to ELISA, LFDs, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products
W. Jin, K. Yamada, M. Ikami, N. Kaji, M. Tokeshi, Y. Atsumi, M. Mizutani, A. Murai, A. Okamoto, T. Namikawa, Y. Baba, M. Ohta, INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 42, S46, S47, Jun. 2013
English, Summary international conference

Nano Medicine based on Nano Packaging
松尾保隆, 加地範匡, 畠山浩人, 渡慶次学, 小暮健太朗, 馬場嘉信, 原島秀吉, 表面, 51, 5, 227-240, 240, May 2013, [Peer-reviewed]
広信社, Japanese, Report scientific journal

ICP-MS連結マイクロ流路による1細胞レベルでの金属元素検出システムの開発
宮崎義之, 安井隆雄, 宮下振一, 稲垣和三, 加地範匡, 梅村知也, 渡慶次学, 馬場嘉信, 化学とマイクロ・ナノシステム研究会誌,, 12, 1, 16-21, 29 Mar. 2013
Japanese, Report scientific journal

小型・高感度マイクロチップ用蛍光検出システムの構築と評価
長谷川寛, 長谷川寛, 松本浩幸, 笠間敏博, 笠間敏博, 加地範匡, 加地範匡, 加地範匡, 近藤治靖, 近藤治靖, 小澤勉, 小澤勉, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 93rd, 1, 2013

超高性能無動力in situ抽出法の創成
岡本行広, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 93rd, 1, 2013

細胞の表層改質を利用したマイクロデバイスによる単一細胞解析
日比野理人, 岡本行広, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 93rd, 2, 2013

ナノ構造体を用いたDNA増幅のリアルタイム無標識検出
安井隆雄, 安井隆雄, 小川謙亮, 小川謙亮, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 堀池靖浩, NILSSON Mats, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 73rd, 2013

リアルタイムDNA増幅の高感度無標識検出法
小川謙亮, 小川謙亮, 安井隆雄, 安井隆雄, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 堀池靖浩, NILSSON Mats, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 93rd, 2, 2013

血中希少循環腫瘍細胞の簡便・安価な非標識検出法および回収法の創成
岡本行広, 長谷哲成, 渡慶次学, 長谷川好規, 馬場嘉信, 日本化学会講演予稿集, 93rd, 1, 2013

単一細胞解析のためのバイオミメティック細胞表層改質法の創成
岡本行広, 渡慶次学, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 73rd, 2013

遺伝子治療用ナノ構造体精製のためのフリーフロー電気泳動マイクロデバイスの開発
重中大輔, 鵜川真美, 加地範匡, 渡慶次学, 秋田英万, 原島秀吉, 馬場嘉信, 日本化学会講演予稿集, 93rd, 2, 2013

ナノバイオデバイスを用いた生体分子の超高速分離
WU Qiong, WU Qiong, 安井隆雄, 安井隆雄, SAKON Rahong, 長島一樹, 柳田剛, 金井真樹, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 川合知二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 93rd, 2, 2013

細胞の物理的破砕のためのナノワイヤデバイスの開発
安井隆雄, 安井隆雄, 伊藤聡, 伊藤聡, 柳田剛, 加地範匡, 加地範匡, HE Yong, RAHONG Sakon, 金井真樹, 長島一樹, 渡慶次学, 渡慶次学, 川合知二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 27th, 2013

音を利用したマイクロ流体デバイスへの小型電力供給システム
内藤豊裕, 内藤豊裕, 内藤豊裕, 加地範匡, 加地範匡, LE GAC Severine, 安井隆雄, 安井隆雄, 渡慶次学, 渡慶次学, VAN DEN BERG Albert, 久保拓也, 大塚浩二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 27th, 2013

ベッドサイド診断を目的としたイムノピラーデバイスの可搬型検出器の開発
笠間敏博, 笠間敏博, 長谷川寛, 長谷川寛, 松本浩幸, 近藤治靖, 近藤治靖, 小澤勉, 小澤勉, 加地範匡, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 27th, 2013

マイクロ流体デバイスを用いるDDSナノキャリアの作製
齋藤竜亮, 安井隆雄, 安井隆雄, 石田晃彦, 谷博文, 加地範匡, 加地範匡, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 28th, 2013

単一細胞解析のためのバイオミメティック細胞表層改質法の創成
岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 高分子学会予稿集(CD-ROM), 62, 1, 2013

積層厚膜SU-8ドライフィルムレジストで成形した深型流路を持つ吸光測定用マイクロフローセル
小野島大介, WANG Jun, 安藝理彦, 有永健児, 加地範匡, 渡慶次学, 藤田省三, 横山直樹, 馬場嘉信, 日本化学会講演予稿集, 93rd, 2, 2013

自己組織化ナノワイヤ構造体を利用したDNAの分離解析
安井隆雄, 安井隆雄, SAKON Rahong, 柳田剛, 加地範匡, 加地範匡, 金井真樹, 長島一樹, 渡慶次学, 渡慶次学, 川合知二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 93rd, 2, 2013

Point-of-care testing system with immuno-pillar devices
笠間敏博, 加地範匡, 馬場嘉信, 長谷川寛, 松本浩幸, 近藤治靖, 小澤勉, 渡慶次学, 電気学会バイオ・マイクロシステム研究会資料, BMS-13, 35-47, 2013

高感度CRP分析のための小型診断システム
笠間敏博, 笠間敏博, 長谷川寛, 長谷川寛, 近藤治靖, 近藤治靖, 小澤勉, 小澤勉, 加地範匡, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 28th, 2013

イムノピラーチップの高性能化
西脇奈菜子, 石田晃彦, 谷博文, 笠間敏博, 笠間敏博, 馬場嘉信, 馬場嘉信, 馬場嘉信, 渡慶次学, 渡慶次学, 渡慶次学, 化学とマイクロ・ナノシステム学会研究会講演要旨集, 27th, 2013

High-speed micro-RNA isolation from DNA fragments by nanopiller array chip
Qiong Wu, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2, 1206, 1208, 2013, [Peer-reviewed]
English, Introduction international proceedings

High throughput purification devices for in vivo applications of gene-delivery multifunctional envelope-type nanodevices
Noritada Kaji, Daisuke Shigenaka, Masami Ukawa, Manabu Tokeshi, Hidetaka Akita, Hideyoshi Harashima, Yoshinobu Baba, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2, 1170, 1172, 2013, [Peer-reviewed]
English, Introduction international proceedings

Development of 3RD generation immuno-pillar device for high sensitive detection of disease markers
N. Nishiwaki, T. Kasama, A. Ishida, H. Tani, Y. Baba, M. Tokeshi, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2, 1164, 1166, 2013, [Peer-reviewed]
English, Introduction international proceedings

Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal
Takao Yasui, Kensuke Ogawa, Noritada Kaji, Mats Nilsson, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2, 1123, 1125, 2013, [Peer-reviewed]
English, Introduction international proceedings

Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break
D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2, 820, 822, 2013, [Peer-reviewed]
English, Introduction international proceedings

連載 超解像顕微鏡の世界：波長の壁を超えて見る(1) 超解像顕微鏡とは：Abbeの法則への挑戦　　　　　　　　　　　　　　　
加地範匡, 渡慶次学, 馬場嘉信, 現代化学, 502, 1, 50-54, Dec. 2012
Japanese, Introduction scientific journal

Portable Liquid Chromatography System Based on Battery-Powered Electroosmotic Pump and Microchip with Packed Column and Electrochemical Detector　　　　　　　　　　　　　　　
Akihiko Ishida, Takehiro Fujimoto, Satoshi Yokokawa, Hirofumi Tani, Manabu Tokeshi, Ichiro Yanagisawa, Proceedings of the MicroTAS 2012 Symposium, 1183, 1185, Oct. 2012, [Peer-reviewed]
English, Summary international conference

On-Chip Bioluminescence Assay of ATP and Kinases Using Immobilized Firefly Luciferase in Three-Dimensional Microfluidic Chip　　　　　　　　　　　　　　　
Hirofumi Tani, Atsuki Morisaki, Akihiko Ishida, Manabu Tokeshi, Proceedings of the MicroTAS 2012 Symposium, 1618, 1620, Oct. 2012, [Peer-reviewed]
English, Summary international conference

エピジェネティクス解析のための一分子DNAメチル化検出技術の開発　　　　　　　　　　　　　　　
岡本 行広, 佐野 竜輝, 加地 範匡, 渡慶次 学, 馬場 嘉信, 日本分析化学会講演要旨集, 61年会, 68, 68, Sep. 2012
(公社)日本分析化学会, Japanese

ナノワイヤデバイスによる生体分子解析　　　　　　　　　　　　　　　
大塚 康平, 安井 隆雄, Rahong Sakon, 柳田 剛, 加地 範匡, 金井 真樹, 長島 一樹, 渡慶次 学, 谷口 正輝, 川合 知二, 馬場 嘉信, 日本分析化学会講演要旨集, 61年会, 191, 191, Sep. 2012
(公社)日本分析化学会, Japanese

マイクロ流体チップによる多機能性エンベロープ型ナノ構造体の作製　　　　　　　　　　　　　　　
加地 範匡, 北添 雄眞, 重中 大輔, 小暮 健太朗, 秋田 英万, 原島 秀吉, 渡慶次 学, 馬場 嘉信, 日本DDS学会学術集会プログラム予稿集, 28回, 182, 182, Jun. 2012
日本DDS学会, Japanese

ナノ構造体を用いたリアルタイムDNA増幅の無標識検出法
小川謙亮, 小川謙亮, 安井隆雄, 安井隆雄, 加地範匡, 加地範匡, 岡本行広, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

フリーフロー電気泳動法を用いた遺伝子治療用ナノデバイスの精製
重中大輔, 鵜川真美, 加地範匡, 岡本行広, 渡慶次学, 秋田英万, 原島秀吉, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

ナノピラー境界面でのDNAトラッピング現象を用いたDNA分離
本山高貴, 安井隆雄, 加地範匡, 岡本行広, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

PMMA製マイクロピラーアレイデバイスによる血球分離
伊藤聡, 伊藤聡, 安井隆雄, 安井隆雄, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 25th, 2012

マイクロデバイスとICP-MSを組み合わせた単一細胞解析法の開発
宮崎義之, 宮崎義之, 安井隆雄, 安井隆雄, 稲垣和三, 岡本行広, 加地範匡, 加地範匡, 梅村知也, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 1, 2012

ナノワイヤデバイスを用いた生体分子の分離
WU Qiong, WU Qiong, 本山高貴, 本山高貴, 安井隆雄, 安井隆雄, SAKON Rahong, 柳田剛, 金井真樹, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 長島一樹, 川合知二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 1, 2012

ナノ構造体による回折現象を用いたDNAの無標識検出
安井隆雄, 小川謙亮, 加地範匡, 岡本行広, 渡慶次学, 堀池靖浩, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

ナノ構造体を用いたDNA無標識検出法
安井隆雄, 安井隆雄, 小川謙亮, 小川謙亮, 加地範匡, 加地範匡, 岡本行広, 渡慶次学, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 72nd, 2012

ナノピラーとナノスリットを組み合わせた新規ナノ構造体による生体分子の超高速分離
呉瓊, 呉瓊, 安井隆雄, 安井隆雄, RAHONG Sakon, 柳田剛, 金井真樹, 長島一樹, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 川合知二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 25th, 2012

迅速・簡便なエピジェネティクス解析のためのマイクロ流体デバイスを利用した1分子DNAメチル化検出
佐野竜輝, 小野島大介, 岡本行広, 加地範匡, 近藤豊, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

マイクロ血球分離デバイスの開発
伊藤聡, 伊藤聡, 安井隆雄, 安井隆雄, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 1, 2012

1分子DNAのマイクロ流体制御下における酵素分解反応の運動学的解析
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

浮遊細胞培養のための環流培養チップの開発
内藤豊裕, 内藤豊裕, 加地範匡, 加地範匡, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム, 11, 1, 2012

ミドリムシによる細胞分離法の創成
岡本行広, 中北行紀, 佐野貴大, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 3, 2012

高集積化マイクロ化学チップのための自律駆動型マイクロバルブの開発
内藤豊裕, 内藤豊裕, 加地範匡, 加地範匡, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

機能性ユーグレナの創成および分離手法への応用
岡本行広, 中北行紀, 佐野貴大, 加地範匡, 加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 72nd, 2012

ナノワイヤデバイスによる1分子DNA解析
本山高貴, 本山高貴, 安井隆雄, 安井隆雄, RAHONG Sakon, 柳田剛, 加地範匡, 加地範匡, 金井真樹, 長島一樹, 岡本行広, 渡慶次学, 渡慶次学, 渡慶次学, 川合知二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 25th, 2012

ICP-MSを用いた単一がん細胞内金属元素量解析のための微粒子挙動制御デバイスの作製
宮崎義之, 宮崎義之, 安井隆雄, 安井隆雄, 稲垣和三, 岡本行広, 加地範匡, 加地範匡, 梅村知也, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 25th, 2012

ハイスループット血漿分離のための渦巻き型マイクロチャネル
森川純平, 森川純平, 加地範匡, 加地範匡, 岡本行広, 渡慶次学, 渡慶次学, 坪田一男, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

バイオミメティックプロセスを利用した単一細胞解析手法の開発
日比野理人, 岡本行広, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

Development of Mass-Producible Rapid Mixer Based on Baker’s Transformation
尾本悠輔, 加藤大香士, 鈴木教和, 安井隆雄, 大里恵子, 加地範匡, 渡慶次学, 馬場嘉信, 酒井康彦, 社本英二, 日本機械学会論文集 B編(Web), 78, 788, 2012

カーボンナノウォールの細胞培養基材への応用
岡本行広, 久保和稔, 久保和稔, 渡邊均, 近藤博基, 渡慶次学, 渡慶次学, 堀勝, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 3, 2012

量子ドットを用いた腫瘍細胞イメージング
中尾早織, 中尾早織, PARK Yeon-Su, 岡本行広, 加地範匡, 加地範匡, 水野正明, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

イムノピラーチップを用いた尿中の糖尿病性腎症マーカーの高感度・高速検出
笠間敏博, 笠間敏博, SUN Miaomiao, 秋山真一, 加地範匡, 加地範匡, 湯澤由紀夫, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 92nd, 2, 2012

マイクロチップを用いた分析手法のための蛍光検出システムの構築と評価
長谷川寛, 長谷川寛, 長谷川寛, 松本浩幸, 笠間敏博, 笠間敏博, 加地範匡, 加地範匡, 加地範匡, 近藤治靖, 近藤治靖, 小澤勉, 小澤勉, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, キャピラリー電気泳動シンポジウム講演要旨集, 32nd, 2012

On-chip electric power generation system from sound of portable music plyers and smartphones towerd portable μTAS
Toyohiro Naito, Noritada Kaji, Séverine Le Gac, Manabu Tokeshi, Albert Van Den Berg, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1852, 1854, 2012, [Peer-reviewed]
English, Summary international conference

Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection
Yukihiro Okamoto, Tatsuki Sano, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1939, 1941, 2012, [Peer-reviewed]
English, Summary international conference

Label-free detection of real-time DNA amplification using nanowall array structures
Kensuke Ogawa, Takao Yasui, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yasuhiro Horiike, Mats Nilsson, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 25, 27, 2012, [Peer-reviewed]
English, Summary international conference

Single DNA manipulation in sublithographic nanowire array chips
Takao Yasui, Sakon Rahong, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kentaro Doi, Manabu Tokeshi, Satovuki Kawano, Tomoji Kawai, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 228, 230, 2012, [Peer-reviewed]
English, Summary international conference

Plasma separation from human blood using spiral microchannels for dry eye treatment
Jumpei Morikawa, Takao Yasui, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Kazuo Tsubota, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 464, 466, 2012, [Peer-reviewed]
English, Summary international conference

Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips
Qiong Wu, Koki Motoyama, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 599, 601, 2012, [Peer-reviewed]
English, Summary international conference

A highly efficient 3D micromixer fabricated by standard soft-lithography equipment
Toyohiro Naito, Rerngchai Arayanarakool, Noritada Kaji, Séverine Le Gac, Manabu Tokeshi, Albert Van Den Berg, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 662, 664, 2012, [Peer-reviewed]
English, Summary international conference

Detection of metallic elements in a single cancer cell using microfluidic devices coupled with ICP-MS
Yoshiyuki Miyazaki, Takao Yasui, Kazumi Inagaki, Yukihiro Okamoto, Noritada Kaji, Tomonari Umemura, Manabu Tokeshi, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 767, 769, 2012, [Peer-reviewed]
English, Summary international conference

Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices
Miaomiao Sun, Toshihiro Kasama, Noritada Kaji, Shin Ichi Akiyama, Yukio Yuzawa, Seiichi Matsuo, Manabu Tokeshi, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 812, 814, 2012, [Peer-reviewed]
English, Summary international conference

Microfluidic rare cancer cell collection with anti-EpCAM antibody modified euglena by phototaxis inside microchannels
Yukihiro Okamoto, Yukinori Nakakita, Takahiro Sano, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1108, 1110, 2012, [Peer-reviewed]
English, Summary international conference

Develpoement of novel circulating tumor cells separation and non-labeling detection by circulating tumor cells' specific properties
Kouhei Ootsuka, Yukihiro Okamoto, Tetsunari Hase, Manabu Tokeshi, Noritada Kaji, Yoshinori Hasegawa, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1123, 1125, 2012, [Peer-reviewed]
English, Summary international conference

Nanopillar parallel-array structure with DNA trapping and torque-assisted escape mode for DNA separation
Takao Yasui, Koki Motoyama, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1186, 1188, 2012, [Peer-reviewed]
English, Summary international conference

Fabrication of PMMA micropillars by reactive ion etching towards separation of white and red blood cells
Satoru Ito, Takao Yasui, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1234, 1236, 2012, [Peer-reviewed]
English, Summary international conference

High throughput purification device for gene delivery multifunctional envelope-type nanodevice
Daisuke Shigenaka, Masami Ukawa, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Hidetaka Akita, Hideyoshi Harashima, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1546, 1548, 2012, [Peer-reviewed]
English, Summary international conference

Graphene forest devices as cell scaffolds for stem cells
Yukihiro Okamoto, Hitoshi Watanabe, Kazutoshi Kubo, Hiroki Kondo, Noritada Kaji, Manabu Tokeshi, Masaru Hori, Yoshinobu Baba, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1633, 1635, 2012, [Peer-reviewed]
English, Summary international conference

ナノ構造体による回折現象を利用した無標識検出法
安井隆雄, 加地範匡, 岡本行広, 渡慶次学, 堀池靖浩, 馬場嘉信, 化学とマイクロ・ナノシステム研究会誌,, 10, 2, 22-23, Nov. 2011
Japanese, Report scientific journal

Electroosmotic Flow in Nanopillar Chips　　　　　　　　　　　　　　　
Takao Yasui, Noritada Kaji, M. Reza Mohamadi, Yukihiro Okamoto, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, ACS Nano, 5, 10, 7775-80, 25 Oct. 2011, [Peer-reviewed]
English, Report scientific journal

急性肝不全マウスにおける移植幹細胞のIn vivoイメージング　　　　　　　　　　　　　　　
湯川 博, 渡辺 将生, 宮本 義孝, 岡本 行広, 加地 範匡, 渡慶次 学, 野口 洋文, 馬場 嘉信, 林 衆治, JSMI Report, 4, 2, 57, 57, May 2011
日本分子イメージング学会, Japanese

急性肝不全マウスにおける移植幹細胞のIn vivoイメージング　　　　　　　　　　　　　　　
湯川 博, 渡辺 将生, 宮本 義孝, 岡本 行広, 加地 範匡, 渡慶次 学, 野口 洋文, 馬場 嘉信, 林 衆治, JSMI Report, 4, 2, 167, 167, May 2011
日本分子イメージング学会, Japanese

ナノ構造体上での幹細胞挙動の観察
KUBO KAZUTOSHI, OKAMOTO YUKIHIRO, YAMAMOTO MASAYA, KAJI NORITADA, TOKESHI MANABU, TABATA YASUHIKO, BABA YOSHINOBU, 日本化学会講演予稿集, 91st, 3, 716, 11 Mar. 2011
Japanese

Tracking Degradations of Single DNA and Protein Molecules in Fluid　　　　　　　　　　　　　　　
D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba, Biophysical Society 55th Annual Meeting, 100, 3, 151a-152a, Mar. 2011, [Peer-reviewed]
English, Summary national conference

量子ドットによる幹細胞のin vivoイメージング　　　　　　　　　　　　　　　
渡辺将生, 湯川博, 鏡味幸真, 加地範匡, 宮本義孝, 岡本行広, 渡慶次学, 林衆治, 馬場嘉信, 生命化学研究レター, 35, 38-42, 01 Feb. 2011
Japanese, Introduction scientific journal

量子ドットを用いた移植幹細胞のin vivoイメージング
渡辺将生, 渡辺将生, 湯川博, 加地範匡, 加地範匡, 宮本義孝, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 林衆治, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 91st, 2, 2011

ナノ空間中のDNAダイナミクス制御に基づく長鎖DNA分離
本山高貴, 本山高貴, 安井隆雄, 安井隆雄, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 23rd, 2011

治療薬物モニタリングチップ:血清サンプルの1ステップ分析
杉浦佳奈子, 岡本行広, 加地範匡, 渡慶次学, 馬場嘉信, 杉浦佳奈子, 岡本行広, 加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 91st, 2, 2011

ミドリムシを利用した細胞分離法の開発(2)
岡本行広, 中北行紀, 佐野貴大, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本分析化学会年会講演要旨集, 60th, 2011

マイクロ化学チップの高集積化に向けた熱応答性バルブの開発
内藤豊裕, 内藤豊裕, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 91st, 2, 2011

マイクロ・ナノ構造体による1分子酵素反応解析
飯嶋和樹, 飯嶋和樹, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 91st, 2, 2011

ナノ構造体を用いた新規無標識バイオセンサの開発
安井隆雄, 加地範匡, 岡本行広, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 91st, 2, 2011

浮遊細胞培養のための還流培養チップの開発
内藤豊裕, 内藤豊裕, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 24th, 2011

磁気ビーズによるイムノピラーチップのマイクロ流路内攪拌
笠間敏博, 笠間敏博, 渡慶次学, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 24th, 2011

熱応答性自立駆動型マイクロバルブの開発
内藤豊裕, 内藤豊裕, ARAYANARAKOOL Rerngchai, LE GAC Severine, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, VAN DEN BERG Albert, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 23rd, 2011

ミドリムシによる細胞分離法の創成
岡本行広, 岡本行広, 佐野貴大, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 91st, 3, 2011

マイクロ流体制御下におけるプロテアーゼ反応の分子的検出
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 91st, 2, 2011

Single molecule enzymatic kinetics in subcellularsized nanospaces using pneumatic valve-assisted atto-liter chamber array devices
K. Iijima, N. Kaji, Y. Okamoto, M. Tokeshi, Y. Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2, 1092, 1094, 2011, [Peer-reviewed]
English, Summary national conference

DNA trapping at the interface of nanopillar and nanopillar-free regions for long DNA separation
Koki Motoyama, Takao Yasui, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 1, 647, 649, 2011, [Peer-reviewed]
English, Summary national conference

Epigenetic analysis of a single DNA molecule by microfluidic device with quantum dot
Tatsuki Sano, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 1, 299, 301, 2011, [Peer-reviewed]
English, Summary national conference

Microfluidic cell separation with antibody modified euglena by using phototaxis mediated migration
Yukihiro Okamoto, Yukinori Nakakita, Takahiro Sano, Jumpei Morikawa, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 3, 1909, 1911, 2011, [Peer-reviewed]
English, Summary national conference

Long-time stress less cell culture chips for non-adhesive cells
Toyohiro Naito, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2, 816, 818, 2011, [Peer-reviewed]
English, Summary national conference

Temperature-driven self-actuated microvalve for PCR
Toyohiro Naito, Rerngchai Arayanarakool, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Séverine Le Gac, Albert Van Den Berg, Y. Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 1, 248, 250, 2011, [Peer-reviewed]
English, Summary national conference

Nanowall array chips for DNA separation
Takao Yasui, Noritada Kaji, Ryo Ogawa, Shingi Hashioka, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 1, 638, 640, 2011, [Peer-reviewed]
English, Summary national conference

Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging
D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 3, 2061, 2063, 2011, [Peer-reviewed]
English, Summary national conference

ナノ構造体上での培養が幹細胞の増殖と分化へ及ぼす影響
KUBO KAZUTOSHI, OKAMOTO YUKIHIRO, YAMAMOTO MASAYA, KAJI NORITADA, TOKESHI MANABU, TABATA YASUHIKO, BABA YOSHINOBU, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 31, 17 Nov. 2010
Japanese

量子ドットによる細胞移植後の幹細胞イメージング　　　　　　　　　　　　　　　
渡辺将生, 湯川博, 鏡味幸真, 加地範匡, 宮本義孝, 岡本行広, 渡慶次学, 林衆治, 馬場嘉信, ナノ学会会報, 9, 1, 7-12, Oct. 2010
Japanese, Technical report

ICP-MSによるマウスに静注した量子ドット標識化脂肪組織由来幹細胞の体内動態の精密測定　　　　　　　　　　　　　　　
高崎 裕加, 渡辺 将生, 湯川 博, 梅村 知也, 加地 範匡, 岡本 行広, 宮本 義孝, 野口 洋文, 渡慶次 学, 林 衆治, 馬場 嘉信, 日本分析化学会講演要旨集, 59年会, 153, 153, Sep. 2010
(公社)日本分析化学会, Japanese

幹細胞治療に対する量子ドットを用いたin vivoイメージング　　　　　　　　　　　　　　　
湯川 博, 渡辺 将生, 鏡味 幸真, 宮本 義孝, 加地 範匡, 渡慶次 学, 野口 洋文, 馬場 嘉信, 林 衆治, JSMI Report, 3, 2, 49, 49, May 2010
日本分子イメージング学会, Japanese

高感度脳腫瘍細胞イメージング方法の開発　　　　　　　　　　　　　　　
岡本 行広, 中尾 早織, 水野 正明, 加地 範匡, 渡慶次 学, 川西 悟基, 榊 裕之, 馬場 嘉信, JSMI Report, 3, 2, 157, 157, May 2010
日本分子イメージング学会, Japanese

幹細胞治療に対する量子ドットを用いたin vivoイメージング　　　　　　　　　　　　　　　
湯川 博, 渡辺 将生, 鏡味 幸真, 宮本 義孝, 加地 範匡, 渡慶次 学, 野口 洋文, 馬場 嘉信, 林 衆治, JSMI Report, 3, 2, 166, 166, May 2010
日本分子イメージング学会, Japanese

Development of high performance separation and analysis method
OKAMOTO Yukihiro, KAJI Noritada, TOKESHI Manabu, BABA Yoshinobu, 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan, 2010, 1, 21, 25, 29 Jan. 2010
Japanese

間隔の異なるナノピラーを集積化したナノバイオデバイスの開発
安井隆雄, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 2, 2010

イムノピラーチップ:血液中の疾病マーカーの多項目同時検出
川上亜矢子, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 2, 2010

ナノピラーチップを用いた簡便なスタッキング法の開発
安井隆雄, 加地範匡, 岡本行広, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 21st, 2010

マイクロ構造体を用いた1分子酵素反応解析
飯嶋和樹, 飯嶋和樹, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 21st, 2010

Development of an integrated microchip for therapeutic drug monitoring
杉浦佳奈子, 杉浦佳奈子, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, Chromatography, 31, Supplement 2, 2010

マイクロバイオデバイスを用いた血漿中DNAの高効率抽出
佐野竜輝, 岡本行広, 加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

マイクロ構造体を用いた1分子酵素反応解析
飯嶋和樹, 飯嶋和樹, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

ナノ構造体を用いた新規無標識検出法の開発
安井隆雄, 加地範匡, 岡本行広, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

ナノ構造体を利用した新規無標識検出法の開発
安井隆雄, 加地範匡, 岡本行広, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, キャピラリー電気泳動シンポジウム講演要旨集, 30th, 2010

CEDIA法を利用した治療薬物モニタリングチップの開発
杉浦佳奈子, 杉浦佳奈子, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 21st, 2010

治療薬物モニタリングチップの開発:反応試薬の集積化と試料の定量採取
杉浦佳奈子, 杉浦佳奈子, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

ヒト全血から血漿の同時分離,定容,希釈を可能とするマイクロ流体システムの開発
森川純平, 森川純平, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

細胞濃縮のためのマイクロ流体デバイスの開発
内藤豊裕, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 2, 2010

マイクロ流体制御下における核酸分解酵素反応の分子的検出
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 90th, 2, 2010

血中薬物濃度分析のためのマイクロ流体デバイスの開発
杉浦佳奈子, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 2, 2010

マイクロ流体デバイスを用いたDNA高次構造転移の1分子解析
鈴木博詞, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本分析化学会年会講演要旨集, 59th, 2010

マイクロ流体デバイスを利用したDNAコンフォメーション変化の1分子解析
鈴木博詞, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 2, 2010

ミドリムシが細胞を分離する
岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本分析化学会年会講演要旨集, 59th, 2010

Development of a Microfluidic System for Simultaneous Separation, Metering, and Dilution of Plasma from Human Whole Blood
森川純平, 森川純平, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, Chromatography, 31, Supplement 2, 2010

温度応答性マイクロバルブの開発
内藤豊裕, 内藤豊裕, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

イムノピラーチップを用いた甲状腺刺激ホルモンの検出
孫苗苗, 孫苗苗, 川上亜矢子, 川上亜矢子, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

マイクロバイオデバイスを用いた血漿中DNAの高効率捕捉
佐野竜輝, 佐野竜輝, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 東京コンファレンス講演要旨集, 2010, 2010

光硬化性樹脂を用いたダイヤフラム型ニューマティックバルブの作製法
内藤豊裕, 内藤豊裕, ARAYANAKOOL Rerngchai, LE GAC Severine, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, VAN DEN BERG Albert, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 21st, 2010

マイクロバイオデバイスを用いた血中DNAの高効率捕捉
佐野竜輝, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 1, 2010

高感度脳腫瘍細胞検出の試み
岡本行広, 中尾早織, 水野正明, 加地範匡, 渡慶次学, 川西悟基, 榊裕之, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 71st, 2010

量子ドット内包リポソームの作製と特性評価
中尾早織, 岡本行広, 岡本行広, 水野正明, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 川西悟基, 榊裕之, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 1, 2010

膜タンパク質解析のための脂質二重膜構築
西川勝彦, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 3, 2010

マイクロ構造体を利用した微小空間における1分子酵素反応解析
飯嶋和樹, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 2, 2010

非標識型タンパク質検出手法を適用した毒素タンパク質の検出
安藝理彦, WANG Jun, 小野島大介, 吉瀬智康, JIN Wanchun, 藤原健志, 有永健児, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 山田景子, 山田景子, 藤田省三, 馬場嘉信, 馬場嘉信, 太田美智男, 太田美智男, 横山直樹, 応用物理学関係連合講演会講演予稿集(CD-ROM), 57th, 2010

マイクロ流体デバイスによる遺伝子治療用ナノデバイスの高速作製
北添雄眞, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 小暮健太郎, 原島秀吉, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 90th, 3, 2010

ミドリムシが細胞を分離する
岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 東京コンファレンス講演要旨集, 2010, 2010

膜タンパク質解析のためのマイクロチャンバーアレイデバイス
西川勝彦, 西川勝彦, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

ミドリムシによる細胞分離の試み
中北行紀, 中北行紀, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

プロテアーゼ反応の分子的リアルタイムトラッキング
小野島大介, 小野島大介, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 22nd, 2010

Tracking of single DNA and protein molecules undergoing enzymatic degradation in fluid
D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3, 1439, 1441, 2010, [Peer-reviewed]
English

国際会議 microTAS2010　　　　　　　　　　　　　　　
渡慶次学, 馬場嘉信, 次世代センサ, 20, 15-18, 2010
Japanese, Technical report

A microdevice with chaotic mixer to constract multifunctional envelope-type nanodevice for delivery system
K. Kitazoe, Y. Okamoto, N. Kaji, M. Tokeshi, K. Kogure, H. Harashima, Y. Baba, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 2, 827, 829, 2010, [Peer-reviewed]
English, Summary national conference

Development of three-step consolidating microchip for therapeutic drug monitoring
K. Sugiura, N. Kaji, Y. Okamoto, M. Tokeshi, Y. Baba, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 2, 806, 808, 2010, [Peer-reviewed]
English, Summary national conference

Real-time observation of dna comformational transitions at a single-molecule level by microfluidic devices
H. Suzuki, N. Kaji, Y. Okamoto, M. Tokeshi, Y. Baba, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3, 1880, 1882, 2010, [Peer-reviewed]
English, Summary national conference

Microfluidic sensor for the detection of DNA or protein by hybridization-based fluorescence enhancement or immunoassay-based fluorescence quenching
J. Wang, M. Aki, D. Onoshima, K. Arinaga, N. Kaji, M. Tokeshi, S. Fujita, N. Yokoyama, Y. Baba, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3, 1514, 1516, 2010, [Peer-reviewed]
English, Summary national conference

Influence of nanostructure on proliferation and differentiation processes of stem cell
Kazutoshi Kubo, Yukihiro Okamoto, Masaya Yamamoto, Noritada Kaji, Manabu Tokeshi, Yasuhiko Tabata, Yoshinobu Baba, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 1, 181, 183, 2010, [Peer-reviewed]
English, Summary national conference

Label-free detection of biomolecules with nanowall arrays
Takao Yasui, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 1, 485, 487, 2010, [Peer-reviewed]
English, Summary national conference

BIOINDUSTRY センシングバイオロジー-生命科学そして医療を支えるセンシング技術 量子ドットを用いた脂肪組織由来幹細胞のバイオイメージング
鏡味幸真, 渡辺将生, 湯川 博, 加地範匡, 岡本行広, 渡慶次学, 林 衆治, 馬場嘉信, BIOINDUSTRY センシングバイオロジー-生命科学そして医療を支えるセンシング技術 量子ドットを用いた脂肪組織由来幹細胞のバイオイメージング, 26, 12, 14, 18, Dec. 2009
シーエムシー出版, Japanese, Others

量子ドットを用いた脂肪組織由来幹細胞のバイオイメージング　　　　　　　　　　　　　　　
鏡味幸真, 渡辺将生, 湯川博, 加地範匡, 岡本行広, 渡慶次学, 林衆治, 馬場嘉信, Bioindustry, 309, (5), 14-18, Dec. 2009
Japanese, Technical report

マイクロチップを利用した簡易細胞アッセイ技術の開発　　　　　　　　　　　　　　　
岡本 行広, 杉浦 佳奈子, 加地 範匡, 渡慶次 学, 馬場 嘉信, 日本バイオマテリアル学会大会予稿集, 31回, 316, 316, Nov. 2009
日本バイオマテリアル学会, Japanese

臨床応用が始まるチップ疾患診断　　　　　　　　　　　　　　　
渡慶次学, 舘知也, 馬場嘉信, 現代化学, 464, (11), 34-38, Oct. 2009
Japanese, Technical report

非標識型タンパク質検出手法の迅速化と高感度化 毒素タンパク質検出への応用　　　　　　　　　　　　　　　
安藝 理彦, 汪 俊, 小野島 大介, 吉瀬 智康, 金 万春, 藤原 健志, 有永 健児, 加地 範匡, 渡慶次 学, 山田 景子, 藤田 省三, 馬場 嘉信, 太田 美智男, 横山 直樹, 日本分析化学会講演要旨集, 58年会, 52, 52, Sep. 2009
(公社)日本分析化学会, Japanese

ANYL 226-Micro- and nanostructures for DNA and protein analysis
Noritada Kaji, Y. Okamoto, Manabu Tokeshi, Yoshinobu Baba, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 238, ., Aug. 2009, [Peer-reviewed]
English, Summary international conference

【ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術 細胞の生存、増殖、機能のコントロールから創薬研究、再生医療まで】細胞周辺環境のための材料加工・利用技術 医療に貢献するナノバイオ技術　　　　　　　　　　　　　　　
岡本 行広, 加地 範匡, 渡慶次 学, 馬場 嘉信, 遺伝子医学MOOK, 別冊, ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術, 161, 168, Aug. 2009
(株)メディカルドゥ, Japanese

マイクロ流体デバイスを利用した血中薬物定量分析
舘 知也, 加地 範匡, 渡慶次 学, 馬場 嘉信, TDM研究, 26, 3, s134, s134, Jun. 2009
(一社)日本TDM学会, Japanese

Development of Immunoassay Chip Toward Practical Applications
TOKESHI Manabu, 電気学会研究会資料. OQD, 光・量子デバイス研究会, 2009, 42, 31, 32, 07 May 2009
Japanese

Velocity gap theory developed for magnifying resolutions without changing separation mechanisms or separation lengths
Yong Zhang, Jun Wang, Yukihiro Okamoto, Manabu Tokeshi, Noritada Kaji, Yoshinobu Baba, Analytical Chemistry, 81, 7, 2745, 2750, Apr. 2009, [Peer-reviewed], [International Magazine]
English

ナノ構造体を用いたDNA解析　　　　　　　　　　　　　　　
安井隆雄, 加地範匡, 岡本行広, 渡慶次学, 馬場嘉信, NEW GLASS, 24, (1), 22-27, Mar. 2009
Japanese, Technical report

新しい感染症検査の動向 マイクロ流路とIgY抗体を用いた黄色ブドウ球菌エンテロトキシンの微量・迅速・超高感度の検出
金 万春, 山田 景子, 岡本 陽, 太田 美智男, 伊神 舞, 渡慶次 学, 加地 範匡, 馬場 嘉信, 木下 圭司, 水谷 誠, 村井 篤嗣, 並河 鷹夫, 日本細菌学雑誌, 64, 1, 106, 106, Feb. 2009
日本細菌学会, Japanese

ナノピラー中における非平衡輸送原理を利用したDNA分離
安井隆雄, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 20th, 2009

リアルタイムPCRチップの開発(2)
内藤豊裕, 八橋愛, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 20th, 2009

細胞パターニングと濃度勾配を利用した細胞アッセイシステムの開発
杉浦佳奈子, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 19th, 2009

Development of a microchip for real-time PCR
八橋愛, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

Development of rapid and high sensitive screening for methicillin-resistant Staphylococcus aureus using immunomagnetic beads in a microfluidic device
川上亜矢子, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 山田景子, 山田景子, 渡慶次学, 渡慶次学, 太田美智男, 太田美智男, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

Cell assay utilizing cell patterning and concentration gradients in microfluidic channels
杉浦佳奈子, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

Development of the new injection method for the microchip electrophoresis with inkjet apparatus
井上陽介, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 1, 2009

Application of anomalous transport phenomena in nanostructures for fast DNA separation
安井隆雄, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

マイクロ流体デバイスを利用したDNA構造転移の1分子解析
鈴木博詞, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 20th, 2009

リアルタイムPCRチップの開発
八橋愛, 内藤豊裕, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 19th, 2009

ナノピラーチップ中での電気浸透流の解析
安井隆雄, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 19th, 2009

Intracellular imaging of cationic liposomes with Quantum Dots
渡辺将生, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

Rapid construction of Multifunctional Envelope-type Nanodevice using microfluidic device
北添雄眞, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 小暮健太郎, 原島秀吉, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

マイクロフルイディクスを用いた血液成分の網羅的解析-細胞・DNA・タンパク質-
渡慶次学, 渡慶次学, 舘知也, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 東京コンファレンス講演要旨集, 2009, 2009

Development of rapid and easy-to-use immunoassay chip
渡慶次学, 渡慶次学, 伊神舞, 加地範匡, 加地範匡, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 1, 2009

ヌクレアーゼ相互作用のマイクロ流体制御と1分子リアルタイム計測
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 中部化学関係学協会支部連合秋季大会講演予稿集, 40th, 2009

Analysis of the Reaction Mechanism of DNA-Binding Protein Using Microchannel and Single-Molecule Tracking
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

Micro Fluidic Structure Optimized by DNA hybridization-Application to protein detection-
安藝理彦, WANG Jun, 小野島大介, 有永健児, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 藤田省三, 馬場嘉信, 馬場嘉信, 横山直樹, 日本化学会講演予稿集, 89th, 1, 2009

Development of a new analysis device for membrane protein
西川勝彦, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 日本化学会講演予稿集, 89th, 2, 2009

ナノデバイスによる生体分子解析
安井隆雄, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 東京コンファレンス講演要旨集, 2009, 2009

DNAハイブリダイゼーションにより最適化した流路構造~たんぱく質検出への応用~
安藝理彦, WANG Jun, 小野島大介, 有永健児, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 藤田省三, 馬場嘉信, 馬場嘉信, 横山直樹, 応用物理学関係連合講演会講演予稿集, 56th, 3, 2009

イムノピラーチップ:血液中の疾病マーカーの検出
川上亜矢子, 田澤英克, 江端智彦, 岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 20th, 2009

マイクロ流体デバイスを利用した多機能性エンベロープ型ナノ構造体の高速作製
北添雄眞, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 小暮健太朗, 原島秀吉, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 20th, 2009

Nanopillars and Nanoballs for DNA Analysis
Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu, NANOFLUIDICS: NANOSCIENCE AND NANOTECHNOLOGY, 179, 6, 179-191, 2009, [Peer-reviewed]
RSC Publishing, English, Report scientific journal

On-chip real-time PCR for genetic tug-of-war (g-TOW) experiment
Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Yukihiro Okamoto, Manabu Tokeshi, Yosinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 627, 629, 2009, [Peer-reviewed]
English, Summary national conference

Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers
Jun Wang, Yong Zhang, Yeon Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 791, 793, 2009, [Peer-reviewed]
English, Summary national conference

Non-equilibrium DNA transport in nanopillar array structures
Takao Yasui, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1198, 1200, 2009, [Peer-reviewed]
English, Summary national conference

Easy cell assay device using two microchannels
Kanako Sugiura, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1243, 1245, 2009, [Peer-reviewed]
English, Summary national conference

Precisely size controlled liposome formation with a microfluidic device
Mitsuru Shibata, Yukihiro Okamoto, Kaji Noritada, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 624, 626, 2009, [Peer-reviewed]
English, Summary national conference

High-throughput microchip electrophoresis analysis with inkjet-injection method
Yukihiro Okamoto, Yousuke Inoue, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 794, 796, 2009, [Peer-reviewed]
English, Summary national conference

Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure
Tomoya Tachi, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 427, 429, 2009, [Peer-reviewed]
English, Summary national conference

Immuno-pillar chip: Multiplex detection of proteins in real samples
Ayako Kawakami, Mai Ikami, Yukihiro Okamoto, Noritada Kaji, Jin Wanchun, Keiko Yamada, Michio Ohata, Hidekatsu Tazawa, Tomohiko Ebata, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 436, 438, 2009, [Peer-reviewed]
English, Summary national conference

A single molecule analysis for the conformational transition of DNA using microfluidics
Hiroshi Suzuki, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 827, 829, 2009, [Peer-reviewed]
English, Summary national conference

A new microdevice to rapidly constract Multifunctional Envelope-type Nanodevice for gene therapy
Katsuma Kitazoe, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Kentaro Kogure, Hideyoshi Harashima, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1267, 1269, 2009, [Peer-reviewed]
English, Summary national conference

Velocity gap theory developed for magnifying resolutions without changing separation mechanisms or separation lengths
Yong Zhang, Jun Wang, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 788, 790, 2009
English, Summary national conference

A simple and low-cost technique for fabricating microchannels and microstructures with high aspect ratio
Daisuke Onoshima, Jun Wang, Michihiko Aki, Kenji Arinaga, Noritada Kaji, Manabu Tokeshi, Shozo Fujita, Naoki Yokoyama, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1587, 1589, 2009, [Peer-reviewed]
English, Summary national conference

Atto- and femto-litre microfabrcated structures for investigating depression of enzyme activity at a single molecule reaction
Kazuki Iijima, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba, Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 854, 856, 2009, [Peer-reviewed]
English, Summary national conference

A versatile method for the surface modification of the microchannels in polymer microchips　　　　　　　　　　　　　　　
Yukihiro Okamoto, Yeon-Su Park, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2008, 892-894, Oct. 2008, [Peer-reviewed]
English, Summary national conference

COLL 134-Aqueous phase fabrication of CdSe-based nanoparticles and their application for cancer cell imaging
Yeon-Su Park, Yukihiro Okamoto, Jun Wang, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 236, ., Aug. 2008, [Peer-reviewed]
English, Summary international conference

治療薬物モニタリングのためのマイクロ化学システムの開発　　　　　　　　　　　　　　　
渡慶次 学, 舘 知也, 加地 範匡, 馬場 嘉信, 日本分析化学会講演要旨集, 57年会, 63, 63, Aug. 2008
(公社)日本分析化学会, Japanese

量子ドットを用いた非ウイルスベクターの細胞内トラフィッキングの可視化　　　　　　　　　　　　　　　
水船 祥吾, 加地 範匡, 渡慶次 学, 馬場 嘉信, 日本分析化学会講演要旨集, 57年会, 98, 98, Aug. 2008
(公社)日本分析化学会, Japanese

Medical Applications Using Microchips
TOKESHI Manabu, KAJI Noritada, BABA Yoshinobu, 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan, 2008, 1, 21, 24, 12 Feb. 2008
Japanese

DNA Analysis Using Nano Devices
YASUI Takao, KAJI Noritada, TOKESHI Manabu, BABA Yoshinobu, 電気学会研究会資料. OQD, 光・量子デバイス研究会, 2008, 1, 13, 16, 08 Feb. 2008
Japanese

square型ナノピラーチップデバイスを用いたrod-like-DNAの分離
安井隆雄, 加地範匡, 加地範匡, 小川涼, 橋岡真義, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 17th, 2008

マイクロチップ電気泳動によるニューキノロン系耐性菌のSNPs解析
吉川枝里, 加地範匡, 加地範匡, 川村久美子, 川村久美子, 太田美智男, 太田美智男, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 17th, 2008

浮遊細胞のためのマイクロ細胞培養チップの開発
内藤豊裕, 加地範匡, 加地範匡, 安藤妙子, 橋岡真義, 佐藤一雄, 堀池靖浩, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 17th, 2008

インクジェット技術を用いた超高性能マイクロチップ電気泳動法の開発
井上陽介, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 中部化学関係学協会支部連合秋季大会講演予稿集, 39th, 2008

ナノ構造体を用いた生体分子解析
安井隆雄, 加地範匡, 加地範匡, 小川涼, 橋岡真義, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 69th, 2008

ナノピラーチップデバイスによるDNA分離
安井隆雄, 加地範匡, 小川涼, 橋岡真義, 堀池靖浩, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 88th, 2, 2008

高電場下におけるナノピラーチップ中でのDNAの泳動挙動解析
安井隆雄, 加地範匡, 加地範匡, 小川涼, 橋岡真義, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 中部化学関係学協会支部連合秋季大会講演予稿集, 39th, 2008

光圧力とシースフローを利用したマイクロ流路内における生死細胞分離
村田聖弥, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 88th, 2, 2008

マイクロチャネルを利用したDNA凝縮の1分子リアルタイム観察
藤吉健太郎, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 88th, 2, 2008

マイクロフルイディクスを利用した制限酵素反応の1分子トラッキング
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 88th, 2, 2008

マイクロチャネルを利用したDNA凝縮過程の1分子観察
藤吉健太郎, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 17th, 2008

分子トラッキングによる制限酵素反応のケモメトリック解析
小野島大介, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 17th, 2008

B型・C型エンテロトキシンの迅速検出
渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 加地範匡, 加地範匡, 伊神舞, 分析化学討論会講演要旨集, 69th, 2008

マイクロ化学チップを用いた新規リポソーム合成法の開発
柴田充, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 88th, 2, 2008

マイクロ化学チップを用いたリポソーム新規作製法の開発
柴田充, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 中部化学関係学協会支部連合秋季大会講演予稿集, 39th, 2008

あらゆるポリマー製マイクロチップに適用可能な簡易表面改質法の開発
岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 69th, 2008

迅速診断のためのイムノアッセイデバイスの開発
伊神舞, 山田景子, 渡慶次学, 加地範匡, 太田美智男, 馬場嘉信, 日本化学会講演予稿集, 88th, 1, 2008

量子ドットによる遺伝子の細胞内トラフィッキングイメージング
間森千春, 加地範匡, 加地範匡, 岡本行広, 岡本行広, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 中部化学関係学協会支部連合秋季大会講演予稿集, 39th, 2008

タンパク質解析チップ用セルロースフィルムコーティング
岡田浩樹, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 88th, 1, 2008

微小空間における1分子酵素反応の解析
加地範匡, 加地範匡, 村原寿, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 69th, 2008

マイクロ化学デバイスによるタンパク質無細胞合成系の構築
川名隆, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 17th, 2008

汎用ポリマー製マイクロチップへの簡易表面改質の試み
岡本行広, 岡本行広, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 17th, 2008

セルロース誘導体フィルムを用いた電気泳動チップへのコーティング法の開発とタンパク質解析への応用
岡田浩樹, 岡田浩樹, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, バイオメディカル分析科学シンポジウム講演要旨集, 21st, 2008

Microfluidic device to construct multifunctional envelope-type nanodevice for gene therapy
K. Kitazoe, N. Kaji, Y. Okamoto, M. Tokeshi, K. Kogure, H. Harashima, Y. Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 1891, 1893, 2008, [Peer-reviewed]
English

Viscosity-tunable polymers for capillary and microchip electrophoresis.
Kaji Noritada, Okamoto Yukihiro, Tokeshi Manabu, Baba Yoshinobu, SEIBUTSU BUTSURI KAGAKU, 52, 3, 95, 99, 2008
Various kinds of functional polymers have been developed for capillary and microchip electrophoresis. A versatile alternative to entangled and random-coiled polymers, Pluronic F127, is a typical functional polymer for a DNA separation matrix in microchip electrophoresis. This temperature-sensitive and viscosity-tunable polymer provided excellent resolutions over a wide range of DNA sizes based on a different separation mechanism compared with conventional polymers such as cellulose-derivatives. In this review, we will describe different type of viscosity-tunable polymers as well as our recent work using Pluronic F127.
, Japanese Electrophoresis Society, Japanese

Quantitative evaluation of dynamic coating on plastic microchips for preventing protein adsorption
M. R. Mohamadi, T. Yasui, N. Kaji, M. Tokeshi, Y. Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 495, 497, 2008, [Peer-reviewed]
English, Summary national conference

Nanotechnology meets plant biotechnology: Carbon nanotubes deliver DNA and incorporate into the plant cell structure
Maged Fouad, Noritada Kaji, Mohammad Jabasini, Manabu Tokeshi, Yoshinobu Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 227, 229, 2008, [Peer-reviewed]
English, Summary national conference

Liposome formation by counter-current flows in microchannels
Mitsuru Shibata, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 1426, 1428, 2008, [Peer-reviewed]
English, Summary national conference

External force responsible Nanogels for microchip electrophoresis of DNA
K. Kondo, N. Kaji, S. Toita, K. Akiyoshi, M. Tokeshi, Y. Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 1132, 1134, 2008, [Peer-reviewed]
English, Summary national conference

Real-time monitoring of conformational transition of DNA at a single molecule level in microfluidic devices
K. Fujiyoshi, N. Kaji, M. Tokeshi, Y. Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 441, 443, 2008, [Peer-reviewed]
English, Summary national conference

Inkjet-based DNA injector for microchip electrophoresis
Yousuke Inoue, N. Kaji, Y. Okamoto, M. Tokeshi, Y. Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 1456, 1458, 2008, [Peer-reviewed]
English, Summary national conference

Rapid and easy-to-use multiplex immunoassay device
Mai Ikami, Manabu Tokeshi, Yukihiro Okamoto, Noritada Kaji, Yoshinobu Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 1111, 1113, 2008, [Peer-reviewed]
English, Summary national conference

Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins
Takao Yasui, Noritada Kaji, Mohamad Reza Mohamadi, Ryo Ogawa, Shingi Hashioka, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 432, 434, 2008, [Peer-reviewed]
English, Summary national conference

A microfluidic molecular tracking system detects a time-to-search DNA sequence to be cleaved by restriction enzymes
D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, 18, 20, 2008, [Peer-reviewed]
English, Summary national conference

マイクロ化学チップを用いた微小流路内におけるTODGAのNd(III)抽出分離特性
伴康俊, 森田泰治, 菊谷善国, 原田雅幸, 池田泰久, 渡慶次学, 日本原子力学会秋の大会予稿集(CD-ROM), 2007, H59, 07 Sep. 2007
Japanese

Determination of human blood glucose levels using microchip electrophoresis.
Eiki Maeda, Masatoshi Kataoka, Mami Hino, Kazuaki Kajimoto, Noritada Kaji, Manabu Tokeshi, Jun-Ichi Kido, Yasuo Shinohara, Yoshinobu Baba, Electrophoresis, 28, 16, 2927, 33, Aug. 2007, [Peer-reviewed], [International Magazine]
A high-performance monitoring system for human blood glucose levels was developed using microchip electrophoresis with a plastic chip. The combination of reductive amination as glucose labeling with fluorescent 2-aminoacridone (AMAC) and glucose-borate complex formation realized the highly selective detection of glucose even in a complex matrix such as a blood sample. The migration time of a single peak, observed on an electropherogram of AMAC-labeled plasma, closely resembled that of glucose standard solution. The treatment of plasma with hexokinase or glucokinase for glucose phosphorylation resulted in a peak shift from approximately 145 to 70 s, corresponding to glucose and glucose-6-phosphate, respectively. A double-logarithm plot revealed a linear relationship between glucose concentration and fluorescence intensity in the range of 1-300 microM of glucose (r(2) = 0.9963; p <0.01), and the detection limit was 0.92 microM. Furthermore, blood glucose concentrations estimated from the standard curves of three subjects were compared with results obtained by conventional colorimetric analysis using glucose dehydrogenase. Good correlation was observed between methods according to simple linear regression analysis (p <0.05). The reproducibility of the assay was about 6.3-9.1% (RSD) and the within-days and between-days reproducibility were 1.6-8.4 and 5.2-7.2%, respectively. This system enables us to determine blood glucose with high sensitivity and accuracy, and will be applicable to clinical diagnosis., English

Microchip-based immunoassay
Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, BUNSEKI KAGAKU, 56, 7, 521, 534, Jul. 2007
Japanese, Book review

Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis
Laili Mahmoudian, Jonas Melin, Mohamad Reza Mohamadi, Keiko Yamada, Michio Ohta, Noritada Kaji, Manabu Tokeshi, Mats Nilsson, Yoshinobu Baba, Chemistry Letters, 36, 3, 396, 397, 05 Mar. 2007, [Peer-reviewed]
English, Report scientific journal

Development of microchip immunoassay using a novel antibody immobilization method
渡慶次 学, 角田 正也, 宮川 堅次, 塗料の研究, 147, 41, 44, Mar. 2007
関西ペイント, Japanese

Rapid bonding of Pyrex glass microchips
Yoshitake Akiyama, Keisuke Morishima, Atsuna Kogi, Yoshikuni Kikutani, Manabu Tokeshi, Takehiko Kitamori, ELECTROPHORESIS, 28, 6, 994, 1001, Mar. 2007
English

バイオ関連トピックス (特集 第10回化学・生命科学マイクロシステム国際会議(μTAS2006)) -- (研究概要)
渡慶次 学, 馬場 嘉信, 化学とマイクロ・ナノシステム, 6, 1, 5, 7, Mar. 2007
化学とマイクロ・ナノシステム研究会, Japanese

Dynamic coating using methylcellulose and polysorbate 20 for nondenaturing electrophoresis of proteins on plastic microchips.
Mohamad Reza Mohamadi, Laili Mahmoudian, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Electrophoresis, 28, 5, 830, 6, Mar. 2007, [Peer-reviewed], [International Magazine]
A dynamic coating using methylcellulose (MC) and a nonionic detergent (polysorbate 20) was developed, which controlled protein adsorption onto the surface of microchannels on a microchip made of poly(methyl methacrylate) (PMMA). Optimum concentration of polysorbate 20 in combination with the range of MC concentrations controlled the protein adsorption onto the microchannel surface, and increased the solubility of the protein samples while facilitating the injection of high concentrations of MC solutions into the microchannels. Higher concentrations of nonionic detergent increased the EOF mobility as opposed to the electrophoretic mobility and caused the electrophoresis to fail. Nondenaturing microchip electrophoresis of protein samples with molecular masses ranging from 20 to 100 kDa were completed in 100 s. Also, successful separation of a BSA sample and its complex with anti-BSA mAb ( 220 kDa) was achieved on a PMMA microchip. The separation exhibited high reproducibility in both migration time (RSD = 1%) and peak area (RSD = 10-15%)., English

Bio-Molecule Imaging by Quantum Dots
KAJI Noritada, TOKESHI Manabu, BABA Yoshinobu, 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan, 2007, 1, 25, 28, 30 Jan. 2007
Japanese

Rapid and simultaneous multiple genotyping of human Y chromosome from a human blood sample by on-chip enzymatic digestion and microchannel array electrophoresis coupled with blood sample pretreatment, and microscale PCR
Lihua Zhang, Toshikatsu Shinka, Yutaka Nakahori, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Sensors and Actuators, B: Chemical, 121, 1, 124, 128, 30 Jan. 2007, [Peer-reviewed]
English, Report scientific journal

DNA分離に対するナノピラーデバイスの特性解析と最適化
安井隆雄, 加地範匡, 加地範匡, 小川涼, 橋岡真義, 渡慶次学, 渡慶次学, 堀池靖浩, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 16th, 2007

タンパク質の高効率マイクロチップ電気泳動解析のためのセルロース誘導体を用いた新規チップコーティング手法の開発
岡田浩樹, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 87th, 2, 2007

マイクロチャネルを利用したB型からA型DNAへのコンフォメーション変化の1分子計測
藤吉健太郎, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 87th, 2, 2007

ナノ構造体を用いた分離分析法
加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 分析化学討論会講演要旨集, 68th, 2007

量子ドット複合体による非ウイルス性ベクターの細胞内導入過程の可視化
水船翔悟, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 87th, 2, 2007

光硬化性樹脂を用いた新規イムノアッセイデバイスの開発
伊神舞, 渡慶次学, 渡慶次学, 加地範匡, 加地範匡, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 15th, 2007

3次元構造を利用したマイクロミキサー
大里恵子, 渡慶次学, 渡慶次学, 加地範匡, 加地範匡, 尾本悠輔, 鈴木教和, 酒井康彦, 社本英二, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 15th, 2007

マイクロ流体デバイスを用いた無細胞タンパク質合成系の構築
川名隆志, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 87th, 2, 2007

量子ドットをプローブとしたDNAの1分子相互作用解析
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会講演予稿集, 87th, 2, 2007

新規イムノアッセイデバイスの開発
渡慶次学, 渡慶次学, 伊神舞, 加地範匡, 加地範匡, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 高分子学会予稿集(CD-ROM), 56, 2 Disk1, 2007

ナノデバイスによる1分子バイオ計測
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 高分子学会予稿集(CD-ROM), 56, 2 Disk1, 2007

微小空間内における酵素反応のモレキュラークラウディング効果
村原寿, 加地範匡, 加地範匡, 渡慶次学, 渡慶次学, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 16th, 2007

光硬化性樹脂を用いた新規イムノアッセイデバイスの評価
伊神舞, 渡慶次学, 渡慶次学, 加地範匡, 加地範匡, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 16th, 2007

A New On-Chip Platform For Rapid And Easy-To-Use Immunoassay
Mai Ikami, Manabu Tokeshi, Noritada Kaji, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 655-657, 657, 2007
English, Summary national conference

Development of new microfluidic immunoassay chips
Tokeshi Manabu, Ikami Mai, Kaji Noritada, Baba Yoshinobu, PROCEEDINGS OF THE FIRST SHENYANG INTERNATIONAL COLLOQUIUM ON MICROFLUIDICS, 199-200, 200, 2007, [Peer-reviewed]
English, Report scientific journal

A novel high efficient and speed construction method of a multi functional envelope-type nano device on microdevice　　　　　　　　　　　　　　　
Hiroshi Kuramoto, Noritada Kaji, Kentarou Kogure, Manabu Tokeshi, Yasuo Shinohara, Hideyoshi Harashima, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 781-783, 2007
English, Summary national conference

Cell Sorting Of Live And Dead Cells By Laser Radiation Pressure And Sheath Flow In Microchannel
Masaya Murata, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 116-118, 118, 2007, [Peer-reviewed]
English, Summary national conference

Molecular Crowding Effect On Enzymatic Reaction In A Fl-Microchamber To Mimic Crowded Intracellular Enviromnent
Hisashi Murahara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 1003-1005, 1005, 2007
Japanese, Summary national conference

Dna Separation By Square Patterned Nanopillar Chips
Takao Yasui, Noritada Kaji, Ryo Ogawa, Shingi Hashioka, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 1207-1209, 1209, 2007
English, Summary national conference

Viscosity-Tunable Polymer For Microchip Electrophoresis
Daisuke Kuroda, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 841-843, 843, 2007
English, Summary national conference

Quantum-Dot-Connected Dna For Single-Molecular Nanobiodevices　　　　　　　　　　　　　　　
Daisuke Onoshima, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 1619-1621, 2007
English, Summary national conference

800 Fold Signal Enhancements By Transient Isotachophoresis In Linear Polymer Solution For Immunoassay Of Hsa On Standard Cross Channel Microchips
Mohamad Reza Mohamadi, Laili Mahmoudian, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 1402-1404, 1404, 2007
English, Summary national conference

Dynamics Measurement Of Structural Change Of Helical Polymer Using Thermal Lens Microscopy And Microfluidic Technique
Keiko Osato, Manabu Tokeshi, Noritada Kaji, Kazuma Mawatari, Takehiko Kitamori, Eiji Yashima, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 1565-1567, 1567, 2007
English, Summary national conference

Micromixer Based On Baker'S Transformation
Keiko Osato, Manabu Tokeshi, Noritada Kaji, Yusuke Omoto, Yasuhiko Sakai, Eiji Shamoto, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 309-311, 311, 2007
English, Summary national conference

Fast Differentiative Quantitation Of Salivary And Pancreatic Amylase Activities In Human Blood By Microchip Electrophoretic Separation Of Substrate/Hydorlysate Coupled With Immunoinhibition　　　　　　　　　　　　　　　
Eiki Maeda, Masatoshi Kataoka, Yasuo Shinohara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2007, 2007, 65-67, 2007
English, Summary national conference

マイクロチップイムノアッセイ　　　　　　　　　　　　　　　
舘知也, 加地範匡, 渡慶次学, 馬場嘉信, 分析化学, 56, 7, 521-534, 2007, [Invited]
Japanese, Report scientific journal

Nanotechnology in Proteome Analysis　　　　　　　　　　　　　　　
Y. Zhang, N. Kaji, M. Tokeshi, Y. Baba, Expert Review of Proteomics, 4, 4, 565-572, 2007, [Peer-reviewed]
English, Report scientific journal

Influences of electroosmotic flows in nanopillar chips on DNA separation: Experimental results and numerical simulations
Noritada Kaji, Akio Oki, Ryo Ogawa, Yuzuru Takamura, Takahiro Nishimoto, Hiroaki Nakanishi, Yasuhiro Horiike, Manabu Tokeshi, Yoshinobu Baba, Israel Journal of Chemistry, 47, 2, 161, 169, 2007, [Peer-reviewed]
English, Report scientific journal

マイクロリアクターを用いた高効率化学合成　　　　　　　　　　　　　　　
渡慶次学, 菊谷善国, バイオインダストリー, 24, 2, 53-58, 2007, [Invited]
Japanese, Report scientific journal

【ナノテクノロジーとバイオセンサ】マイクロチップ分析関連 マイクロ・ナノバイオデバイスによるDNA・蛋白質解析
渡慶次 学, 加地 範匡, 馬場 嘉信, 臨床検査, 50, 12, 1567, 1575, Nov. 2006
(株)医学書院, Japanese

マイクロ・ナノデバイスによる生体分子解析と疾患診断
加地 範匡, 渡慶次 学, 馬場 嘉信, 現代化学, 425, 31, 38, Aug. 2006, [Invited]
東京化学同人, Japanese, Report scientific journal

マイクロチャネルを利用したB型からA型DNAへのコンフォメーション変化の直接観察
加地範匡, 藤吉健太郎, 渡慶次学, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 13th, 2006

マイクロチップゲルシフトアッセイによるDNAとタンパク質の相互作用解析
井上園子, 片岡正俊, 片岡正俊, 篠原康雄, 加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本分析化学会年会講演要旨集, 55th, 2006

マイクロチップ電気泳動による糖鎖解析
Fuquan Dang, 掛樋一晃, 石川満, 加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 日本分析化学会年会講演要旨集, 55th, 2006

マイクロチャネルを用いた無細胞タンパク質合成系の構築
川名隆志, 加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 13th, 2006

量子ドットを用いた1分子DNA-タンパク質間相互作用のオンチップ計測
小野島大介, 加地範匡, 渡慶次学, 馬場嘉信, 日本化学会バイオテクノロジー部会シンポジウム講演要旨集, 9th, 2006

マイクロチップゲルシフトアッセイを用いたDNA-NFκBの相互作用に関する研究
井上園子, 片岡正俊, 片岡正俊, 篠原康雄, 加地範匡, 渡慶次学, 馬場嘉信, 馬場嘉信, 化学とマイクロ・ナノシステム研究会講演要旨集, 13th, 2006

Continuous flow chemical processing on a microchip using microunit operations and a multiphase flow network (vol 47, pg 434, 2005)
M Tokeshi, T Kitamori, PROGRESS IN NUCLEAR ENERGY, 48, 2, 182, 182, 2006
English, Others

On-chip construction of a multifunctional envelope-type nano device for a non-viral gene delivery system
Hiroshi Kuramoto, Noritada Kaji, Kentarou Kogure, Manabu Tokeshi, Yasuo Shinohara, Hideyoshi Harashima, Yoshinobu Baba, Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS2006, 1005, 1007, 2006, [Peer-reviewed]
English

Direct monitoring of B- to a-DNA conformational transition at a single molecule level induced by ethanol in microfluidic devices
Kentaro Fujiyoshi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS2006, 1396, 1398, 2006, [Peer-reviewed]
English

ナノバイオデバイスのバイオ応用　　　　　　　　　　　　　　　
渡慶次 学, 加地範匡, 細胞工学, 25, 873-877, 2006, [Invited]
Japanese, Report scientific journal

Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB
Sonoko Inoue, Masatoshi Kataoka, Yasuo Shinohara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS2006, 903, 905, 2006
English, Summary national conference

A microchip-based fluorescence polarization immunoassay
Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS2006, 816, 818, 2006
English, Summary national conference

Determination of blood sugar using microchip electrophoresis
Eiki Maeda, Masatoshi Kataoka, Yasuo Shinohara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS2006, 813, 815, 2006
English, Summary national conference

Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis
Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS2006, 401, 403, 2006
English, Summary national conference

APPLICATION OF 1,4-DIOXANE TO ENHANCING SELECTIVITY IN ANALYSIS OF GLYCOSAMINOGLYCAN DISACCHARIDES BY MICROCHIP ELECTROPHORESIS　　　　　　　　　　　　　　　
Yong Zhang, Guichen Ping, Bingmei Zhu, Hiroshi Chuman, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba, Micro Total Analysis System 2006, μTAS2006, 2006
English, Summary national conference

Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip
Mohamad Reza Mohamadi, Laili Mahmoudian, Noritada Kaji, Manabu Tokeshi, Hiroshi Chuman, Yoshinobu Baba, Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS2006, 353, 355, 2006
English, Summary national conference

細胞培養のためのマイクロチップ温度制御装置の開発
田中有希, 西中正弘, 馬渡和真, 渡慶次学, 渡慶次学, 北森武彦, 北森武彦, 分析化学討論会講演要旨集, 66th, 16, 30 Apr. 2005
Japanese

Chemical Process in Microchannels Using Gas-Liquid Two-Phase Flow
TOKESHI Manabu, KITAMORI Takehiko, Japanese journal of multiphase flow, 19, 1, 25, 30, 15 Mar. 2005
日本混相流学会, Japanese

Thermal Lens Microscope
MAWATARI Kazuma, HIKI Shinichiro, FUKUZAWA Takashi, YAMAGUCHI Jun, HATTORI Akihiko, TOKESHI Manabu, KITAMORI Takehiko, Japanese journal of optics, 33, 12, 708, 714, 10 Dec. 2004
応用物理学会分科会日本光学会, Japanese

Ultratrace Analysis-Single Molecule Detection Methods
MAWATARI Kazuma, TOKESHI Manabu, KITAMORI Takehiko, ぶんせき, 357, 506, 512, 05 Sep. 2004
日本分析化学会, Japanese