2025年10月, 日本プロテインホスファターゼ研究, 日本プロテインホスファターゼ研究会功労賞　　　　　　　　　　　　　　　
癌抑制タンパク質p53誘導性Ser/ThrホスファターゼPPM1D
坂口和靖

2024年10月, 日本ペプチド学会, 日本ペプチド学会賞　　　　　　　　　　　　　　　
ペプチド科学を基盤とした生命現象における多量体化および複合体形成を介した機能制御機構の解明とその応用
坂口和靖

2010年10月, 日本生化学会, 2010 年度（第18 回） JB 論文賞　　　　　　　　　　　　　　　
PPM1D430, a Novel Alternative Splicing Variant of the Human PPM1D, can Dephosphorylate p53 and Exhibits Specific Tissue Expression
中馬吉郎;栗橋渉;水上洋平;梨本健紘;八木寛陽;坂口和靖

Bacterial Systematic Genetics and Integrated Multi-Omics: Beyond Static Genomics Toward Predictive Models
Tatsuya Sakaguchi, Yuta Irifune, Rui Kamada, Kazuyasu Sakaguchi
International Journal of Molecular Sciences, 26, 19, 9326, 9326, MDPI AG, 2025年09月24日, ［査読有り］, ［最終著者, 責任著者］
英語, 研究論文（学術雑誌）, The field of bacterial systems biology is rapidly advancing beyond static genomic analyses, and moving toward dynamic, integrative approaches that connect genetic variation with cellular function. This review traces the progression from genome-wide association studies (GWAS) to multi-omics frameworks that incorporate transcriptomics, proteomics, and interactome mapping. We emphasize recent breakthroughs in high-resolution transcriptomics, including single-cell, spatial, and epitranscriptomic technologies, which uncover functional heterogeneity and regulatory complexity in bacterial populations. At the same time, innovations in proteomics, such as data-independent acquisition (DIA) and single-bacterium proteomics, provide quantitative insights into protein-level mechanisms. Experimental and AI-assisted strategies for mapping protein–protein interactions help to clarify the architecture of bacterial molecular networks. The integration of these omics layers through quantitative trait locus (QTL) analysis establishes mechanistic links between single-nucleotide polymorphisms and systems-level phenotypes. Despite persistent challenges such as bacterial clonality and genomic plasticity, emerging tools, including deep mutational scanning, microfluidics, high-throughput genome editing, and machine-learning approaches, are enhancing the resolution and scope of bacterial genetics. By synthesizing these advances, we describe a transformative trajectory toward predictive, systems-level models of bacterial life. This perspective opens new opportunities in antimicrobial discovery, microbial engineering, and ecological research.

Dominant‐Negative Effects of p53 R337 Variants in Li–Fraumeni Syndrome: Impact on Tetramer Formation and Transcriptional Activity
Rui Kamada, Shuya Sakaguchi, Madoka Kanno, Takaaki Ozawa, Natsumi Nakagawa, James G. Omichinski, Kazuyasu Sakaguchi
ChemBioChem, Wiley, 2025年07月24日, ［査読有り］, ［最終著者, 責任著者］
研究論文（学術雑誌）, Li–Fraumeni syndrome (LFS) is an inherited cancer predisposition disorder caused by heterozygous TP53 mutations. Among these, missense mutations at Arg337—such as R337C and R337H—are common in LFS patients. Although many studies have characterized individual p53 variants in LFS, the impact of tetramerization domain (TD) mutations on wild‐type (WT) p53 function remains unclear. Herein, a novel FRET‐based assay system that enables the simultaneous detection of heterotetramer formation and p53‐dependent transcriptional activity in live cells is developed. These results show that the heteromultimerization of the R337C variant with WT p53 is only slightly reduced compared to WT homotetramers, yet its transcriptional activity is diminished by over 50%. In contrast, the R337H variant forms heterotetramers at near‐normal levels but exhibits markedly compromised transcriptional activity. These findings reveal a previously unrecognized dominant‐negative‐like effect, suggesting reduced p53 function is due not only to decreased tetramer formation but also to diminished heterotetramer stability. Moreover, the LFS‐associated p53TD variants show a greater loss of activity against the low‐affinity, apoptosis‐inducing bax response element than against the high‐affinity, cell cycle arrest‐related CDKN1A response element. Collectively, this study demonstrates that p53TD mutations can exert dominant‐negative effects, advancing the understanding of p53 heteromultimer function in LFS pathogenesis. These mechanistic insights into p53 heterotetramer stability may not only inform genetic screening strategies for LFS but also support future therapeutic approaches aimed at restoring p53 function by stabilizing mutant tetramers.

Targeted inhibition of WIP1 and histone H3K27 demethylase activity synergistically suppresses neuroblastoma growth
Diana Treis, Kristina Ihrmark Lundberg, Nicola Bell, Panagiotis Alkinoos Polychronopoulos, Conny Tümmler, Emma Åkerlund, Stefania Aliverti, Ingrid Lilienthal, Adena Pepich, Brinton Seashore-Ludlow, Kazuyasu Sakaguchi, Per Kogner, John Inge Johnsen, Malin Wickström
Cell Death & Disease, 16, 1, Springer Science and Business Media LLC, 2025年04月19日, ［査読有り］
研究論文（学術雑誌）, Abstract

High-risk neuroblastoma frequently exhibits segmental gain of chromosome 17q, including the locus of PPM1D, which encodes the phosphatase WIP1, a regulator of p53 activity, DNA repair, and apoptosis. High expression of PPM1D is correlated to poor prognosis, and genetic or pharmacologic inhibition of WIP1 suppresses neuroblastoma growth. Here, we show that combining drugs that target WIP1 and H3K27 demethylation induces synergistic cytotoxicity in neuroblastoma. We screened 527 different compounds together with inhibitors of WIP1 and identified a strong cytotoxic synergism between the WIP1 inhibitor SL-176 and GSK-J4, a specific inhibitor of the H3K27 demethylase JMJD3. Viability assays in neuroblastoma cell lines and treatment of tumor spheroids confirmed the synergistic effect of combining SL-176 with GSK-J4. Immunoblot experiments demonstrated a marked effect on WIP1 downstream targets and apoptosis markers, while qPCR showed a synergistic upregulation of p53 downstream targets PUMA and p21. RNA sequencing revealed a vast number of differentially expressed genes, suggesting a pervasive effect of this drug combination on transcription, with enrichment of pathways involved in DNA damage response. Finally, this drug combination was confirmed to reduce tumor growth in zebrafish xenograft experiments. In conclusion, the combination of the WIP1 inhibitor SL-176 and the epigenetic modifier GSK-J4 induces synergistic cytotoxicity in neuroblastoma cells by potentiating p53 downstream effects.

Biomineralization through a Symmetry-Controlled Oligomeric Peptide
Tatsuya Sakaguchi, Natsumi Nakagawa, Kenta Mine, Jose Isagani B. Janairo, Rui Kamada, James G. Omichinski, Kazuyasu Sakaguchi
Biomimetics, 8, 8, 606, 606, MDPI AG, 2023年12月14日, ［査読有り］, ［最終著者, 責任著者］
研究論文（学術雑誌）, Biomineralization peptides are versatile tools for generating nanostructures since they can make specific interactions with various inorganic metals, which can lead to the formation of intricate nanostructures. Previously, we examined the influence that multivalency has on inorganic structures formed by p53 tetramer-based biomineralization peptides and noted a connection between the geometry of the peptide and its ability to regulate nanostructure formation. To investigate the role of multivalency in nanostructure formation by biomineralization peptides more thoroughly, silver biomineralization peptides were engineered by linking them to additional self-assembling molecules based on coiled-coil peptides and multistranded DNA oligomers. Under mild reducing conditions at room temperature, these engineered biomineralization peptides self-assembled and formed silver nanostructures. The trimeric forms of the biomineralization peptides were the most efficient in forming a hexagonal disk nanostructure, with both the coiled-coil peptide and DNA-based multimeric forms. Together, the results suggest that the spatial arrangement of biomineralization peptides plays a more important role in regulating nanostructure formation than their valency.

Highly Similar Tetramerization Domains from the p53 Protein of Different Mammalian Species Possess Varying Biophysical, Functional and Structural Properties.
Shuya Sakaguchi, Natsumi Nakagawa, Haytham M Wahba, Junya Wada, Rui Kamada, James G Omichinski, Kazuyasu Sakaguchi
International journal of molecular sciences, 24, 23, 2023年11月22日, ［査読有り］, ［最終著者, 責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, The p53 protein is a transcriptional regulatory factor and many of its functions require that it forms a tetrameric structure. Although the tetramerization domain of mammalian p53 proteins (p53TD) share significant sequence similarities, it was recently shown that the tree shrew p53TD is considerably more thermostable than the human p53TD. To determine whether other mammalian species display differences in this domain, we used biophysical, functional, and structural studies to compare the properties of the p53TDs from six mammalian model organisms (human, tree shrew, guinea pig, Chinese hamster, sheep, and opossum). The results indicate that the p53TD from the opossum and tree shrew are significantly more stable than the human p53TD, and there is a correlation between the thermostability of the p53TDs and their ability to activate transcription. Structural analysis of the tree shrew and opossum p53TDs indicated that amino acid substitutions within two distinct regions of their p53TDs can dramatically alter hydrophobic packing of the tetramer, and in particular substitutions at positions corresponding to F341 and Q354 of the human p53TD. Together, the results suggest that subtle changes in the sequence of the p53TD can dramatically alter the stability, and potentially lead to important changes in the functional activity, of the p53 protein.

Bilayer Hydrogel Composed of Elastin-Mimetic Polypeptides as a Bio-Actuator with Bidirectional and Reversible Bending Behaviors
Rui Kamada, Hiromitsu Miyazaki, Jose Isagani Janairo, Yoshiro Chuman, Kazuyasu Sakaguchi
Molecules, 2023年07月
研究論文（学術雑誌）

Development of a fluorescence reporter system to quantify transcriptional activity of endogenous p53 in living cells.
Tatsuki Tsuruoka, Emiri Nakayama, Takuya Endo, Shingo Harashima, Rui Kamada, Kazuyasu Sakaguchi, Toshiaki Imagawa
Journal of cell science, 2023年05月22日, ［査読有り］, ［責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）, The tumor suppressor p53 plays a central role in cellular stress responses by regulating transcription of multiple target genes. The temporal dynamics of p53 are thought to be important for its function: it encode input information and are decoded to induce distinct cellular phenotypes. However it remains unclear to what extent the temporal dynamics of p53 reflects the activity of p53-induced gene expression. In this study, we report a multiplexed reporter system that allows us to visualize the transcriptional activity of p53 at the single cell level. Our reporter system features simple and sensitive observation of the transcriptional activity of endogenous p53 to the response elements of various target genes. Using this system, we show that the transcriptional activation of p53 exhibits strong cell-to-cell heterogeneity. The transcriptional activation of p53 by etoposide is highly dependent on the cell cycle but not by UV. Finally, we show that our reporter system allows simultaneous visualization of the transcriptional activity of p53 and cell cycle. Our reporter system can thus be a useful tool for studying biological processes involving the p53 signaling pathway.

Ribosomal protein uL30 undergoes phase separation with nucleophosmin and regulates nucleolar formation in the absence of RNA.
Itsumi Tani, Yui Oikawa, Seiyo Doi, Jose Isagani B Janairo, Rui Kamada, Kazuyasu Sakaguchi
Biochemical and biophysical research communications, 642, 35, 40, 2023年01月29日, ［査読有り］, ［最終著者, 責任著者］, ［国際誌］
英語, 研究論文（学術雑誌）

Lipid Droplet Formation Is Regulated by Ser/Thr Phosphatase PPM1D via Dephosphorylation of Perilipin 1
Rui Kamada, Sae Uno, Nozomi Kimura, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
International Journal of Molecular Sciences, 23, 19, 12046, 12046, MDPI AG, 2022年10月10日
研究論文（学術雑誌）, Hypertrophy and hyperplasia of white adipocytes induce obesity, leading to diseases such as type 2 diabetes and hypertension, and even cancer. Hypertrophy of white adipocytes is attributed to the excessive storage of the energy form of triglycerides in lipid droplets (LDs). LDs are fat storage organelles that maintain whole-body energy homeostasis. It is important to understand the mechanism of LD formation for the development of obesity therapy; however, the regulatory mechanisms of LD size and formation are not fully understood. In this study, we demonstrated that the PPM family phosphatase PPM1D regulates LD formation. PPM1D specific inhibitor, SL-176 significantly decreased LD formation via two different pathways: dependent of and independent of adipocyte-differentiation processes. In the mature white adipocytes after differentiation, LD formation was found to be controlled by PPM1D via dephosphorylation of Ser511 of perilipin 1. We found that inhibition of PPM1D in mature white adipocytes significantly reduced the size of the LDs via dephosphorylation of Ser511 of perilipin 1 but did not change the lipolysis sensitivity and the total amount of lipid in cells. Collectively, the results of this study provide evidence that PPM1D plays an important role in LD formation in mature adipocytes.

Stereoselective Interaction of Helix-Hairpin-Helix Motif Peptides with DNA Structure
Rui Kamada, Shuya Sakaguchi, Shoma Kura, Kaori Takamatsu, Tetsuya Yamamoto, Toshiaki Imagawa, Kazuyasu Sakaguchi
Chemistry Letters, 51, 10, 1029, 1032, The Chemical Society of Japan, 2022年10月05日
研究論文（学術雑誌）

Role of active site arginine residues in substrate recognition by PPM1A
Itsumi Tani, Shogo Ito, Yukiko Shirahata, Yutaka Matsuyama, James G. Omichinski, Yasuyuki Shimohigashi, Rui Kamada, Kazuyasu Sakaguchi
Biochemical and Biophysical Research Communications, 581, 1, 5, Elsevier BV, 2021年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Reversible protein phosphorylation is a key mechanism for regulating numerous cellular events. The metal-dependent protein phosphatases (PPM) are a family of Ser/Thr phosphatases, which uniquely recognize their substrate as a monomeric enzyme. In the case of PPM1A, it has the capacity to dephosphorylate a variety of substrates containing different sequences, but it is not yet fully understood how it recognizes its substrates. Here we analyzed the role of Arg33 and Arg186, two residues near the active site, on the dephosphorylation activity of PPM1A. The results showed that both Arg residues were critical for enzymatic activity and docking-model analysis revealed that Arg186 is positioned to interact with the substrate phosphate group. In addition, our results suggest that which Arg residue plays a more significant role in the catalysis depends directly on the substrate.

PPM1D Is a Therapeutic Target in Childhood Neural Tumors
Jelena Milosevic, Diana Treis, Susanne Fransson, Gabriel Gallo-Oller, Baldur Sveinbjörnsson, Nina Eissler, Keiji Tanino, Kazuyasu Sakaguchi, Tommy Martinsson, Malin Wickström, Per Kogner, John Inge Johnsen
Cancers, 13, 23, 6042, 6042, MDPI AG, 2021年11月30日
研究論文（学術雑誌）, Childhood medulloblastoma and high-risk neuroblastoma frequently present with segmental gain of chromosome 17q corresponding to aggressive tumors and poor patient prognosis. Located within the 17q-gained chromosomal segments is PPM1D at chromosome 17q23.2. PPM1D encodes a serine/threonine phosphatase, WIP1, that is a negative regulator of p53 activity as well as key proteins involved in cell cycle control, DNA repair and apoptosis. Here, we show that the level of PPM1D expression correlates with chromosome 17q gain in medulloblastoma and neuroblastoma cells, and both medulloblastoma and neuroblastoma cells are highly dependent on PPM1D expression for survival. Comparison of different inhibitors of WIP1 showed that SL-176 was the most potent compound inhibiting medulloblastoma and neuroblastoma growth and had similar or more potent effects on cell survival than the MDM2 inhibitor Nutlin-3 or the p53 activator RITA. SL-176 monotherapy significantly suppressed the growth of established medulloblastoma and neuroblastoma xenografts in nude mice. These results suggest that the development of clinically applicable compounds inhibiting the activity of WIP1 is of importance since PPM1D activating mutations, genetic gain or amplifications and/or overexpression of WIP1 are frequently detected in several different cancers.

Metal-dependent Ser/Thr protein phosphatase PPM family: Evolution, structures, diseases and inhibitors
Rui Kamada, Fuki Kudoh, Shogo Ito, Itsumi Tani, Jose Isagani B. Janairo, James G. Omichinski, Kazuyasu Sakaguchi
Pharmacology & Therapeutics, 215, 107622, 107622, Elsevier BV, 2020年11月, ［査読有り］, ［招待有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Protein phosphatases and kinases control multiple cellular events including proliferation, differentiation, and stress responses through regulating reversible protein phosphorylation, the most important post-translational modification. Members of metal-dependent protein phosphatase (PPM) family, also known as PP2C phosphatases, are Ser/Thr phosphatases that bind manganese/magnesium ions (Mn2+/Mg2+) in their active center and function as single subunit enzymes. In mammals, there are 20 isoforms of PPM phosphatases: PPM1A, PPM1B, PPM1D, PPM1E, PPM1F, PPM1G, PPM1H, PPM1J, PPM1K, PPM1L, PPM1M, PPM1N, ILKAP, PDP1, PDP2, PHLPP1, PHLPP2, PP2D1, PPTC7, and TAB1, whereas there are only 8 in yeast. Phylogenetic analysis of the DNA sequences of vertebrate PPM isoforms revealed that they can be divided into 12 different classes: PPM1A/PPM1B/PPM1N, PPM1D, PPM1E/PPM1F, PPM1G, PPM1H/PPM1J/PPM1M, PPM1K, PPM1L, ILKAP, PDP1/PDP2, PP2D1/PHLPP1/PHLPP2, TAB1, and PPTC7. PPM-family members have a conserved catalytic core region, which contains the metal-chelating residues. The different isoforms also have isoform specific regions within their catalytic core domain and terminal domains, and these regions may be involved in substrate recognition and/or functional regulation of the phosphatases. The twenty mammalian PPM phosphatases are involved in regulating diverse cellular functions, such as cell cycle control, cell differentiation, immune responses, and cell metabolism. Mutation, overexpression, or deletion of the PPM phosphatase gene results in abnormal cellular responses, which lead to various human diseases. This review focuses on the structures and biological functions of the PPM-phosphatase family and their associated diseases. The development of specific inhibitors against the PPM phosphatase family as a therapeutic strategy will also be discussed.

Characterization of a C-Terminal SUMO-Interacting Motif Present in Select PIAS-Family Proteins.
Mathieu Lussier-Price, Xavier H Mascle, Laurent Cappadocia, Rui Kamada, Kazuyasu Sakaguchi, Haytham M Wahba, James G Omichinski
Structure (London, England : 1993), 28, 5, 573, 585, 2020年05月05日, ［国際誌］
英語, 研究論文（学術雑誌）, The human PIAS proteins are small ubiquitin-like modifier (SUMO) E3 ligases that participate in important cellular functions. Several of these functions depend on a conserved SUMO-interacting motif (SIM) located in the central region of all PIAS proteins (SIM1). Recently, it was determined that Siz2, a yeast homolog of PIAS proteins, possesses a second SIM at its C terminus (SIM2). Sequence alignment indicates that a SIM2 is also present in PIAS1-3, but not PIAS4. Using biochemical and structural studies, we demonstrate PIAS-SIM2 binds to SUMO1, but that phosphorylation of the PIAS-SIM2 or acetylation of SUMO1 alter this interaction in a manner distinct from what is observed for the PIAS-SIM1. We also show that the PIAS-SIM2 plays a key role in formation of a UBC9-PIAS1-SUMO1 complex. These results provide insights into how post-translational modifications selectively regulate the specificity of multiple SIMs found in the PIAS proteins by exploiting the plasticity built into the SUMO-SIM binding interface.

Acetylation of SUMO1 Alters Interactions with the SIMs of PML and Daxx in a Protein-Specific Manner
Xavier H. Mascle, Christina Gagnon, Haytham M. Wahba, Mathieu Lussier-Price, Laurent Cappadocia, Kazuyasu Sakaguchi, James G. Omichinski
Structure, 28, 2, 157, 168.e5, Elsevier BV, 2020年02月
研究論文（学術雑誌）

The tetramerization domain of the tree shrew p53 protein displays unique thermostability despite sharing high sequence identity with the human p53 protein.
Nakagawa N, Sakaguchi S, Nomura T, Kamada R, Omichinski JG, Sakaguchi K
Biochemical and biophysical research communications, 521, 3, 681, 686, 2019年11月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, The p53 protein plays a number of roles in protecting organisms from different genotoxic stresses and this includes DNA damage induced by acetaldehyde, a metabolite of alcohol. Since the common tree shrew ingests high levels of alcohol as part of its normal diet, this suggests that its p53 protein may possess unique properties. Using a combination of biophysical and modeling studies, we demonstrate that the tetramerization domain of the tree shrew p53 protein is considerably more stable than the corresponding domain from humans despite sharing almost 90% sequence identity. Based on modeling and mutagenesis studies, we determine that a glutamine to methionine substitution at position 354 plays a key role in this difference. Given the link between stability of the p53 tetramerization domain and its transcriptional activity, the results suggest that this enhanced stability could lead to important consequences at p53-regulated genes in the tree shrew.

Inhibition of lipid droplet formation by Ser/Thr protein phosphatase PPM1D inhibitor, SL-176
Rui Kamada, Nozomi Kimura, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
PLOS ONE, 14, 2, e0212682, 2019年02月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Obesity is a worldwide public health problem, which is associated with various severe diseases including diabetes, hypertension, atherosclerosis, and cancer. Recent studies have revealed that combination treatment of several different compounds using low doses is effective to reduce side effects. Thus, there is a need to develop an efficient inhibitor for reducing lipid droplets with a divergent target/pathway. Ser/Thr protein phosphatase PPM1D is involved in cellular metabolic processes and is a promising target for anti-obesity treatment. We have previously developed a potent and specific PPM1D inhibitor, SL-176. In this study, we demonstrated that significant reduction of lipid droplet formation in adipocytes by the PPM1D specific inhibitor, SL-176. Using Oil-red O staining and fluorescent imaging of lipid droplet, we found that treatment of SL-176 significantly suppressed lipid droplet formation of 3T3-L1 cells both in amount and in size. SL-176 also repressed mRNA and protein expression of PPARγ and C/EBPα, adipogenic markers, at nontoxic conditions. Thus, SL-176 is a unique and potent inhibitor of lipid droplet formation that acts via PPM1D, a novel target toward inhibiting adipocyte differentiation.

PPM1D enhances retinoic acid-induced differentiation in human embryonic carcinoma cell line
Ogasawara, S, Chuman, Y, Michiba, T, Kamada, R, Imagawa, T, Sakaguchi, K
J. Biochem., 165, 6, 471, 477, 2019年, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, The protein phosphatase PPM1D (Wip1) was originally identified as a p53 target product. Activation of PPM1D through various mechanism promotes the tumorigenic potential of various cancers by suppressing p53 and other DNA damage response proteins. New functions of PPM1D have recently been revealed in physiological processes such as cell differentiation. However, the regulatory mechanisms of signalling pathway to maintain stemness and induce cell differentiation are still unclear. Here we report that PPM1D modulates retinoic acid (RA) signalling. PPM1D knockdown resulted in decreased alkaline phosphatase activity of the human teratocarcinoma cell line NT2/D1. Inhibition of PPM1D-induced cell differentiation and decreased gene expression of the stem cell marker Oct-4 (POU5F1). RA-induced cell differentiation was promoted by reducing PPM1D activity. RA treatment elicited activation of the MEK-ERK pathway and induced rapid and transient activation of the extracellular signal-regulated kinase 1/2 (ERK-1/2). PPM1D dephosphorylated a phosphopeptide with the TEY motif in ERK-1/2 in vitro. Moreover, phosphorylation of ERK-1/2 was facilitated by PPM1D inhibition. Our study shows that PPM1D plays an important role in maintaining the undifferentiation state and a new function in RA-induced ERK regulation and cell differentiation.

Interferon Stimulation Creates Chromatin Marks And Establishes Transcriptional Memory
Rui Kamada, Wenjing Yang, Yubo Zhang, Mira C. Patel, Yanqin Yang, Ryota Ouda, Anup Dey, Yoshiyuki Wakabayashi, Kazuyasu Sakaguchi, Takashi Fujita, Tomohiko Tamura, Jun Zhu, Keiko Ozato
Proceedings of the National Academy of Sciences of the USA, 115, 39, E9162, E9171, 2018年09月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Epigenetic memory for signal-dependent transcription has remained elusive. So far, the concept of epigenetic memory has been largely limited to cell-autonomous, preprogrammed processes such as development and metabolism. Here we show that IFNβ stimulation creates transcriptional memory in fibroblasts, conferring faster and greater transcription upon restimulation. The memory was inherited through multiple cell divisions and led to improved antiviral protection. Of ∼2,000 IFNβ-stimulated genes (ISGs), about half exhibited memory, which we define as memory ISGs. The rest, designated nonmemory ISGs, did not show memory. Surprisingly, mechanistic analysis showed that IFN memory was not due to enhanced IFN signaling or retention of transcription factors on the ISGs. We demonstrated that this memory was attributed to accelerated recruitment of RNA polymerase II and transcription/chromatin factors, which coincided with acquisition of the histone H3.3 and H3K36me3 chromatin marks on memory ISGs. Similar memory was observed in bone marrow macrophages after IFNγ stimulation, suggesting that IFN stimulation modifies the shape of the innate immune response. Together, external signals can establish epigenetic memory in mammalian cells that imparts lasting adaptive performance upon various somatic cells.

Mutant p53-Expressing Cells Undergo Necroptosis via Cell Competition with the Neighboring Normal Epithelial Cells.
Hirotaka Watanabe, Kojiro Ishibashi, Hiroki Mano, Sho Kitamoto, Nanami Sato, Kazuya Hoshiba, Mugihiko Kato, Fumihiko Matsuzawa, Yasuto Takeuchi, Takanobu Shirai, Susumu Ishikawa, Yuka Morioka, Toshiaki Imagawa, Kazuyasu Sakaguchi, Suguru Yonezawa, Shunsuke Kon, Yasuyuki Fujita
Cell reports, 23, 13, 3721, 3729, 2018年06月26日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, p53 is a tumor suppressor protein, and its missense mutations are frequently found in human cancers. During the multi-step progression of cancer, p53 mutations generally accumulate at the mid or late stage, but not in the early stage, and the underlying mechanism is still unclear. In this study, using mammalian cell culture and mouse ex vivo systems, we demonstrate that when p53R273H- or p53R175H-expressing cells are surrounded by normal epithelial cells, mutant p53 cells undergo necroptosis and are basally extruded from the epithelial monolayer. When mutant p53 cells alone are present, cell death does not occur, indicating that necroptosis results from cell competition with the surrounding normal cells. Furthermore, when p53R273H mutation occurs within RasV12-transformed epithelia, cell death is strongly suppressed and most of the p53R273H-expressing cells remain intact. These results suggest that the order of oncogenic mutations in cancer development could be dictated by cell competition.

Synthesis of bimetallic PdAg nanoparticles through an oligomerization- controlled biomineralization peptide
Jose Isagani B. Janairo, Kazuyasu Sakaguchi
Materials Science Forum, 928, 77, 82, Trans Tech Publications Ltd, 2018年
英語, 研究論文（国際会議プロシーディングス）

Quantitative single cell analysis for transcriptional activity of p53 hetero-tetramers between wild-type protein and oligomerization domain
Yu Toguchi, Rui Kamada, Madoka Kanno, Toshiaki Imagawa, Kazuyasu Sakaguchi
Chemistry Letters, 47, 2, 217, 220, Chemical Society of Japan, 2018年, ［査読有り］
英語, 研究論文（学術雑誌）

Synergic strategies for the enhanced self-assembly of biomineralization peptides for the synthesis of functional nanomaterials
Jose Isagani B. Janairo, Tatsuya Sakaguchi, Kenta Mine, Rui Kamada, Kazuyasu Sakaguchi
Protein and Peptide Letters, 25, 1, 4, 14, Bentham Science Publishers B.V., 2018年01月01日, ［査読有り］, ［招待有り］
英語, 研究論文（学術雑誌）

Inhibition of Ser/Thr phosphatase PPM1D induces neutrophil differentiation in HL-60 cells
Rui Kamada, Fuki Kudoh, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
JOURNAL OF BIOCHEMISTRY, 162, 4, 303, 308, 2017年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Heterochiral Jun and Fos bZIP peptides form a coiled-coil heterodimer that is competent for DNA binding
Rui Kamada, Natsumi Nakagawa, Taiji Oyama, Kazuyasu Sakaguchi
JOURNAL OF PEPTIDE SCIENCE, 23, 7-8, 644, 649, 2017年07月, ［査読有り］
英語, 研究論文（学術雑誌）

Oligomerization enhances the binding affinity of a silver biomineralization peptide and catalyzes nanostructure formation
Tatsuya Sakaguchi, Jose Isagani B. Janairo, Mathieu Lussier-Price, Junya Wada, James G. Omichinski, Kazuyasu Sakaguchi
SCIENTIFIC REPORTS, 7, 1, 1400, 2017年05月, ［査読有り］
英語, 研究論文（学術雑誌）

Tetramer Formation of Tumor Suppressor Protein p53: Structure, Function, and Applications
Kamada, R, Toguchi, Y, Nomura, T, Sakaguchi, K
Biopolymers: Peptide Science, 106, 4, 598, 612, 2016年11月, ［査読有り］, ［招待有り］
英語, 研究論文（学術雑誌）

PPM1D controls nucleolar formation by up-regulating phosphorylation of nucleophosmin
Yuuki Kozakai, Rui Kamada, Junya Furuta, Yuhei Kiyota, Yoshiro Chuman, Kazuyasu Sakaguchi
SCIENTIFIC REPORTS, 6, 33272, 2016年09月, ［査読有り］
英語, 研究論文（学術雑誌）

Patterning nanofibrils through the templated growth of multiple modified amyloid peptides
Hiroki Sakai, Ken Watanabe, Fuki Kudoh, Rui Kamada, Yoshiro Chuman, Kazuyasu Sakaguchi
SCIENTIFIC REPORTS, 6, 31993, 2016年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells
Rui Kamada, Fumi Tano, Fuki Kudoh, Nozomi Kimura, Yoshiro Chuman, Ayumi Osawa, Kosuke Namba, Keiji Tanino, Kazuyasu Sakaguchi
PLOS ONE, 11, 8, e0160625, 2016年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Quantitative Correlation between the Protein Expression Level in Escherichia Coli and Thermodynamic Stability of Protein In Vitro
Junya Wada, Hiromitsu Miyazaki, Rui Kamada, Kazuyasu Sakaguchi
CHEMISTRY LETTERS, 45, 2, 185, 187, 2016年02月, ［査読有り］
英語, 研究論文（学術雑誌）

Effect of C-terminal Region of Ser/Threonine Phosphatase PPM1D on its Location.　　　　　　　　　　　　　　　
Kudoh, F, Kamada, R, Ito, S, Kiyota, Y, Sakaguchi, K
Peptide Sci., 2015, 221, 222, 2016年, ［査読有り］
英語, 研究論文（学術雑誌）

Novel inhibitors targeting PPM1D phosphatase potently suppress cancer cell proliferation
Sari Ogasawara, Yuhei Kiyota, Yoshiro Chuman, Ayano Kowata, Fumihiko Yoshimura, Keiji Tanino, Rui Kamada, Kazuyasu Sakaguchi
BIOORGANIC & MEDICINAL CHEMISTRY, 23, 19, 6246, 6249, 2015年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Antiproliferative Activity of Silver Nanoplates on Human Promyelocytic Leukemia Cell Lines
Tatsuya Sakaguchi, Kenta Mine, Fuki Kudoh, Rui Kamada, Kazuyasu Sakaguchi
CHEMISTRY LETTERS, 44, 3, 327, 329, 2015年03月, ［査読有り］
英語, 研究論文（学術雑誌）

Synthesis of yellow and red fluorescent 1,3a, 6a-triazapentalenes and the theoretical investigation of their optical properties
Kosuke Namba, Ayumi Osawa, Akira Nakayama, Akane Mera, Fumi Tano, Yoshiro Chuman, Eri Sakuda, Tetsuya Taketsugu, Kazuyasu Sakaguchi, Noboru Kitamura, Keiji Tanino
CHEMICAL SCIENCE, 6, 2, 1083, 1093, 2015年, ［査読有り］
英語, 研究論文（学術雑誌）

Structural and Functional Characterization of the Phosphorylation-Dependent Interaction between PML and SUMO1
Laurent Cappadocia, Xavier H. Mascle, Veronique Bourdeau, Samuel Tremblay-Belzile, Malik Chaker-Margot, Mathieu Lussier-Price, Junya Wada, Kazuyasu Sakaguchi, Muriel Aubry, Gerardo Ferbeyre, James G. Omichinski
STRUCTURE, 23, 1, 126, 138, 2015年01月, ［査読有り］
英語, 研究論文（学術雑誌）

Function of Proto-oncogene Product PPM1D and Development of PPM1D Inhibitors for Cancer Chemotherapy
Kamada R, Chuman Y, Kozakai Y, Sakaguchi K
Seikagaku. The Journal of Japanese Biochemical Society, 87, 5, 531, 538, 日本生化学会, 2015年, ［査読有り］
日本語

Inhibition of C-terminal truncated PPM1D enhances the effect of doxorubicin on cell viability in human colorectal carcinoma cell line
Yuuki Kozakai, Rui Kamada, Yuhei Kiyota, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 24, 24, 5593, 5596, 2014年12月, ［査読有り］
英語, 研究論文（学術雑誌）

Effects of Buffer on the Structure and Catalytic Activity of Palladium Nanomaterials Formed by Biomineralization
Jose Isagani B. Janairo, Kazuyasu Sakaguchi
CHEMISTRY LETTERS, 43, 8, 1315, 1317, 2014年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Effects of biomineralization peptide topology on the structure and catalytic activity of Pd nanomaterials
Jose Isagani B. Janairo, Tatsuya Sakaguchi, Kenji Hara, Atsushi Fukuoka, Kazuyasu Sakaguchi
CHEMICAL COMMUNICATIONS, 50, 66, 9259, 9262, 2014年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Formation of functionalized nanowires by control of self-assembly using multiple modified amyloid peptides
Hiroki Sakai, Ken Watanabe, Yuya Asanomi, Yumiko Kobayashi, Yoshiro Chuman, Lihong Shi, Takuya Masuda, Thomas Wyttenbach, Michael T. Bowers, Kohei Uosaki, Kazuyasu Sakaguchi
Advanced Functional Materials, 23, 39, 4881, 4887, WILEY-VCH Verlag, 2013年10月18日, ［査読有り］
英語, 研究論文（学術雑誌）

Effects of E/Z Configuration of Fluoroalkene-containing HDAC Inhibitors on Selectivity for HDAC Isoforms
Yoshiro Chuman, Mariko Ueyama, Satoshi Sano, Fei Wu, Yuhei Kiyota, Takayoshi Higashi, Satoshi Osada, Kazuyasu Sakaguchi
CHEMISTRY LETTERS, 42, 8, 833, 835, 2013年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Inhibition of tumor suppressor protein p53-dependent transcription by a tetramerization domain peptide via hetero-oligomerization
Junya Wada, Rui Kamada, Toshiaki Imagawa, Yoshiro Chuman, Kazuyasu Sakaguchi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 22, 8, 2780, 2783, 2012年04月, ［査読有り］
英語, 研究論文（学術雑誌）

癌原遺伝子産物PPM1Dの相互作用因子の同定と核小体形成における機能解析
小境 夕紀, 清田 雄平, 八木 寛陽, 中馬 吉郎, 坂口 和靖
日本プロテオーム学会大会要旨集, 2012, 138, 138, 日本プロテオーム学会（日本ヒトプロテオーム機構）, 2012年
日本語

A small molecule inhibitor of p53-inducible protein phosphatase PPM1D
Yagi Hiroaki, Chuman Yoshiro, Kozakai Yuuki, Imagawa Toshiaki, Takahashi Yu, Yoshimura Fumihiko, Tanino Keiji, Sakaguchi Kazuyasu
Bioorganic & Medicinal Chemistry Letters, 22, 1, 729, 732, Elsevier BV, 2012年, ［査読有り］
研究論文（学術雑誌）

Phosphatase assay for multi-phosphorylated substrates using phosphatase specific-motif antibody
Yoshiro Chuman, Kanako Iizuka, Takeshi Honda, Hitoshi Onoue, Yasuyuki Shimohigashi, Kazuyasu Sakaguchi
JOURNAL OF BIOCHEMISTRY, 150, 3, 319, 325, 2011年09月, ［査読有り］
英語, 研究論文（学術雑誌）

Formation process and structure of polyproline self-assembled monolayer on gold surface
Han Ying, Noguchi Hidenori, Kazuyasu Sakaguchi, Uosaki Kohei
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 241, 2011年03月27日, ［査読有り］

Effective method for formation of functionalized nanowires using amyloid peptides
Sakai Hiroki, Watanabe Ken, Chuman Yoshiro, Uosaki Kohei, Sakaguchi Kazuyasu
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 241, 2011年03月27日, ［査読有り］

Cancer-associated p53 tetramerization domain mutants: quantitative analysis reveals a low threshold for tumor suppressor inactivation.
Kamada, R, Nomura, T, Anderson, C. W, Sakaguchi, K
J. Biol. Chem., 286, 252, 258, 2011年, ［査読有り］
英語, 研究論文（学術雑誌）

Probing phenylalanine environments in oligomeric structures with pentafluorophenylalanine and cyclohexylalanine.
Nomura, T, Kamada, R, Ito, I, Sakamoto, K, Chuman, Y, Ishimori, K, Shimohigashi, Y, Sakaguchi, K
Biopolymers, 95, 6, 410, 419, 2011年, ［査読有り］

Novel chemical inhibitors specific for p53-inducible protein phosphatase PPM1D
Yagi Hiroaki, Chuman Yoshiro, Yoshimura Fumihiko, Tanino Keiji, Sakaguchi Kazuyasu
Abstracts of Papers of the American Chemical Society, 241, 2011年, ［査読有り］

Design of Potent Inhibitors of Human RAD51 Recombinase Based on BRC Motifs of BRCA2 Protein: Modeling and Experimental Validation of a Chimera Peptide
Julian Nomme, Axelle Renodon-Corniere, Yuya Asanomi, Kazuyasu Sakaguchi, Alicja Z. Stasiak, Andrzej Stasiak, Bengt Norden, Vinh Tran, Masayuki Takahashi
JOURNAL OF MEDICINAL CHEMISTRY, 53, 15, 5782, 5791, 2010年08月
英語, 研究論文（学術雑誌）

Enhancement of transcriptional activity of mutant p53 tumor suppressor protein through stabilization of tetramer formation by calix[6]arene derivatives
Rui Kamada, Wataru Yoshino, Takao Nomura, Yoshiro Chuman, Toshiaki Imagawa, Takanori Suzuki, Kazuyasu Sakaguchi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20, 15, 4412, 4415, 2010年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Drastic effects on fibril formation of amyloid-$β$ peptides by the addition of amino acid residue units to the termini
Asanomi Yuya, Kobayashi Yumiko, Sakai Hiroki, Masuda Takuya, Chen Xinjiang, Chuman Yoshiro, Uosaki Kohei, Sakaguchi Kazuyasu
Protein and peptide letters, 17, 4, 458, 463, 2010年04月, ［査読有り］
英語, 研究論文（学術雑誌）

Fluoroalkene Modification of Mercaptoacetamide-based Histone Deacetylase Inhibitors
OSADA S, SANO S, KODAMA H, UEYAMA M, CHUMAN Y, SAKAGUCHI K
Bioorg. Med. Chem., 18, 2, 605, 611, 2010年, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Inhibitors of histone deacetylases (HDAC) are emerging as a promising class of anti-cancer agents. The mercaptoacetoamide-based inhibitors are reported to be less toxic than hydroxamate and are worthy of further consideration. Therefore, we have designed a series of analogs as potential inhibitors of HDACs, in which the mercaptoacetamide group was replaced by (mercaptomethyl)fluoroalkene, and their HDAC inhibitory activity was evaluated. Subnanomolar inhibition was observed for all synthetic compounds.

Tetramerization of tumor suppressor protein p53
Rui Kamada, Kazuyasu Sakaguchi
Seikagaku, 82, 6, 484, 493, 2010年, ［査読有り］
日本語, 研究論文（学術雑誌）

Characterization of a new cancer-associated mutant of p53 with a missense mutation (K351N) in the tetramerization domain
Michela Muscolini, Elisa Montagni, Silvana Caristi, Takao Nomura, Rui Kamada, Silvia Di Agostino, Marco Corazzari, Mauro Piacentini, Giovanni Blandino, Antonio Costanzo, Kazuyasu Sakaguchi, Loretta Tuosto
CELL CYCLE, 8, 20, 3396, 3405, 2009年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Transcriptional Activity of p53 in Individual Living Mammalian Cells.
Imagawa, T, Terai, T, Yamada, Y, Kamada, R, Sakaguchi, K
Anal. Biochem., 387, 249, 256, 2009年, ［査読有り］
英語, 研究論文（学術雑誌）

Effects of tumor-associated mutations in the p53 tetramerization domain on oligomerization state and transcriptional activity.
Kamada, R, Terai, T, Nomura, T, Chuman, Y, Imagawa, T, Sakaguchi, K
Adv. Exp. Med. Biol., 611, 567, 568, 2009年, ［査読有り］
英語, 研究論文（学術雑誌）

Oxidation of methionine residue at hydrophobic core destabilizes p53 tetrameric structure
NOMURA Takao, KAMADA Rui, ITO Issaku, CHUMAN Yoshiro, SHIMOHIGASHI Yasuyuki, SAKAGUCHI Kazuyasu
Biopolymers, 91, 1, 78, 84, 2009年, ［査読有り］

PPM1D430, a Novel Alternative Splicing Variant of the Human PPM1D, can Dephosphorylate p53 and Exhibits Specific Tissue Expression
Yoshiro Chuman, Wataru Kurihashi, Yohei Mizukami, Takehiro Nashimoto, Hiroaki Yagi, Kazuyasu Sakaguchi
JOURNAL OF BIOCHEMISTRY, 145, 1, 1, 12, 2009年01月, ［査読有り］
英語, 研究論文（学術雑誌）

Differential Recognition of Phosphorylated Transactivation Domains of p53 by Different p300 Domains
POLLEY Smarajit, POLLEY Smarajit, GUHA Soumi, ROY Neeladri Sekhar, KAR Sanchari, SAKAGUCHI Kazuyasu, CHUMAN Yoshiro, SWAMINATHAN V, KUNDU Tapas, ROY Siddhartha
J. Mol. Biol., 376, 1, 8, 12, 2008年, ［査読有り］

Characterization of the Active Site and a Unique Uncompetitive Inhibitor of the PPM1-type Protein Phosphatase PPM1D
Chuman, Y, Yagi, H, Fukuda, T, Nomura, T, Matsukizono, M, Shimohigashi, Y, Sakaguchi K
Protein Pept. Lett., 15, 938, 948, 2008年, ［査読有り］

PILLARS OF IMMUNOLOGY
Stern Lawrence J, Hunt Donald F, Henderson Robert A, Shabanowitz Jeffrey, Sakaguchi Kazuyasu, Michel Hanspeter, Sevilir Noelle, Cox Andrea L, Appella Ettore, Engelhard Victor H
J Immunol, 179, 2665, 2007年, ［査読有り］

Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies
Takeshi Honda, Ayami Matsushima, Kazunori Sumida, Yoshiro Chuman, Kazuyasu Sakaguchi, Hitoshi Onoue, Ian A. Meinertzhagen, Yasuyuki Shimohigashi, Miki Shimohigashi
JOURNAL OF COMPARATIVE NEUROLOGY, 499, 3, 404, 421, 2006年11月, ［査読有り］
英語, 研究論文（学術雑誌）

Unfolding, aggregation, and amyloid formation by the tetramerization domain from mutant p53 associated with lung cancer.
Higashimoto Y, Asanomi Y, Takakusagi S, Lewis MS, Uosaki K, Durell SR, Anderson CW, Appella E, Sakaguchi K
Biochemistry, 45, 6, 1608, 1619, 2006年02月, ［査読有り］

Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase
K Abe, S Kaya, K Taniguchi, Y Hayashi, T Imagawa, M Kikumoto, K Oiwa, K Sakaguchi
JOURNAL OF BIOCHEMISTRY, 138, 3, 293, 301, 2005年09月, ［査読有り］
英語, 研究論文（学術雑誌）

Thr-774 (transmembrane segment M5), Val-920 (M8), and Glu-954 (M9) are involved in Na+ transport, and Gln-923 (m8) is essential for Na,K-ATPaseActivity
T Imagawa, T Yamamoto, S Kaya, K Sakaguchi, K Taniguchi
JOURNAL OF BIOLOGICAL CHEMISTRY, 280, 19, 18736, 18744, 2005年05月, ［査読有り］
英語, 研究論文（学術雑誌）

Mechanism of Amyloid-Like Fibrillar Aggregation of Mutant Peptide of p53 Tetramerization Domain
ASANOMI Yuya, TAKAKUSAGI Satoru, CHUMAN Yoshiro, KAYA Shunji, IMAGAWA Toshiaki, UOSAKI Kohei, SAKAGUCHI Kazuyasu
Peptide science : proceedings of the ... Japanese Peptide Symposium, 2004, 0, 313, 316, 2005年03月
英語, 研究論文（国際会議プロシーディングス）

Site-directed affinity-labeling of delta opioid receptors by SNpys-containing enkephalin and dynorphin analogues
K Isozaki, H Fukahori, T Honda, N Shirasu, K Okada, T Nose, K Sakaguchi, Y Shimohigashi
LETTERS IN PEPTIDE SCIENCE, 10, 5-6, 511, 522, 2003年, ［査読有り］
英語, 研究論文（学術雑誌）

Gamma interferon triggers interaction between ICSBP (IRF-8) and TEL, recruiting the histone deacetylase HDAC3 to the interferon-responsive element
T Kuwata, C Gongora, Y Kanno, K Sakaguchi, T Tamura, T Kanno, Basrur, V, R Martinez, E Appella, T Golub, K Ozato
MOLECULAR AND CELLULAR BIOLOGY, 22, 21, 7439, 7448, 2002年11月, ［査読有り］
英語, 研究論文（学術雑誌）

Structural requirements of nociceptin antagonist Ac-RYYRIK-NH2 for receptor binding
M Kawano, K Okada, T Honda, T Nose, K Sakaguchi, T Costa, Y Shimohigashi
JOURNAL OF PEPTIDE SCIENCE, 8, 10, 561, 569, 2002年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Human p53 is phosphorylated on serines 6 and 9 in response to DNA damage-inducing agents.
Higashimoto Y, Saito S, Tong XH, Hong A, Sakaguchi K, Appella E, Anderson CW
The Journal of biological chemistry, 275, 30, 23199, 23203, 2000年07月, ［査読有り］

Damage-mediated phosphorylation of human p53 threonine 18 through a cascade mediated by a casein 1-like kinase - Effect on Mdm2 binding
K Sakaguchi, S Saito, Y Higashimoto, S Roy, CW Anderson, E Appella
JOURNAL OF BIOLOGICAL CHEMISTRY, 275, 13, 9278, 9283, 2000年03月, ［査読有り］
英語, 研究論文（学術雑誌）

Identification of gel-separated tumor marker proteins by mass spectrometry
AC Bergman, T Benjamin, A Alaiya, M Waltham, K Sakaguchi, B Franzen, S Linder, T Bergman, G Auer, E Appella, PJ Wirth, H Jornvall
ELECTROPHORESIS, 21, 3, 679, 686, 2000年02月, ［査読有り］
英語, 研究論文（学術雑誌）

Phosphorylation of human p53 by p38 kinase coordinates N-terminal phosphorylation and apoptosis in response to UV radiation
DV Bulavin, S Saito, MC Hollander, K Sakaguchi, CW Anderson, E Appella, AJ Fornace
EMBO JOURNAL, 18, 23, 6845, 6854, 1999年12月, ［査読有り］
英語, 研究論文（学術雑誌）

Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas
Y Chuman, AC Bergman, T Ueno, S Saito, K Sakaguchi, AA Alaiya, B Franzen, T Bergman, D Arnott, G Auer, E Appella, H Jornvall, S Linder
FEBS LETTERS, 462, 1-2, 129, 134, 1999年11月, ［査読有り］
英語, 研究論文（学術雑誌）

Calreticulin and calreticulin fragments are endothelial cell inhibitors that suppress tumor growth
SE Pike, L Yao, J Setsuda, KD Jones, B Cherney, E Appella, K Sakaguchi, H Nakhasi, CD Atreya, J Teruya-Feldstein, P Wirth, G Gupta, G Tosato
BLOOD, 94, 7, 2461, 2468, 1999年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Calcium-dependent interaction of S100B with the C-terminal domain of the tumor suppressor p53.
Delphin C, Ronjat M, Deloulme JC, Garin G, Debussche L, Higashimoto Y, Sakaguchi K, Baudier J
The Journal of biological chemistry, 274, 15, 10539, 10544, 1999年04月, ［査読有り］

Protein kinase-dependent overexpression of the nuclear protein pirin in c-JUN and RAS transformed fibroblasts
AC Bergman, AA Alaiya, W Wendler, B Binetruy, M Shoshan, K Sakaguchi, T Bergman, U Kronenwett, G Auer, E Appella, H Jornvall, S Linder
CELLULAR AND MOLECULAR LIFE SCIENCES, 55, 3, 467, 471, 1999年03月, ［査読有り］
英語, 研究論文（学術雑誌）

Effects of mutations on tetramer formation of tumor suppressor protein p53
Y Higashimoto, MS Lewis, P Kennedy, AM Gronenborn, GM Clore, E Appella, K Sakaguchi
PEPTIDE SCIENCE - PRESENT AND FUTURE, 303, 305, 1999年, ［査読有り］
英語, 研究論文（国際会議プロシーディングス）

Vasostatin, a calreticulin fragment, inhibits angiogenesis and suppresses tumor growth
SE Pike, L Yao, KD Jones, B Cherney, E Appella, K Sakaguchi, H Nakhasi, J Teruya-Feldstein, P Wirth, G Gupta, G Tosato
JOURNAL OF EXPERIMENTAL MEDICINE, 188, 12, 2349, 2356, 1998年12月, ［査読有り］
英語, 研究論文（学術雑誌）

Identification of new melanoma epitopes on melanosomal proteins recognized by tumor infiltrating T lymphocytes restricted by HLA-A1, -A2, and -A3 alleles
Y Kawakami, PF Robbins, Wang, X, JP Tupesis, MR Parkhurst, XQ Kang, K Sakaguchi, E Appella, SA Rosenberg
JOURNAL OF IMMUNOLOGY, 161, 12, 6985, 6992, 1998年12月, ［査読有り］
英語, 研究論文（学術雑誌）

Posttranslational modifications involved in the DNA damage response.
CW Anderson, E Appella, K Sakaguchi
JOURNAL OF PROTEIN CHEMISTRY, 17, 6, 527, 527, 1998年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Phosphorylation of p53: a novel pathway for p53 inactivation in human T-cell lymphotropic virus type 1-transformed cells
CA Pise-Masison, M Radonovich, K Sakaguchi, E Appella, JN Brady
JOURNAL OF VIROLOGY, 72, 8, 6348, 6355, 1998年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Dilation of the human immunodeficiency virus-1 envelope glycoprotein fusion pore revealed by the inhibitory action of a synthetic peptide from gp41
Munoz-Barroso, I, S Durell, K Sakaguchi, E Appella, R Blumenthal
JOURNAL OF CELL BIOLOGY, 140, 2, 315, 323, 1998年01月, ［査読有り］
英語, 研究論文（学術雑誌）

An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli
ML Cai, Y Huang, K Sakaguchi, GM Clore, AM Gronenborn, R Craigie
JOURNAL OF BIOMOLECULAR NMR, 11, 1, 97, 102, 1998年01月, ［査読有り］
英語, 研究論文（学術雑誌）

DNA damage induces phosphorylation of the amino terminus of p53
JD Siliciano, CE Canman, Y Taya, K Sakaguchi, E Appella, MB Kastan
GENES & DEVELOPMENT, 11, 24, 3471, 3481, 1997年12月, ［査読有り］
英語, 研究論文（学術雑誌）

Increased expression of $α$-enolase in c-jun transformed rat fibroblasts without increased activation of plasminogen
Bergman Ann-Charlotte, Linder Christina, Sakaguchi Kazuyasu, Sten-Linder Margareta, Alaiya Ayodele A, Franz{\'e}n Bo, Shoshan Maria C, Bergman Tomas, Wiman Bj{\"o}rn, Auer Gert, other
FEBS letters, 417, 1, 17, 20, 1997年11月, ［査読有り］
英語, 研究論文（学術雑誌）

Phosphorylation of serine 392 stabilizes the tetramer formation of tumor suppressor protein p53
K Sakaguchi, H Sakamoto, MS Lewis, CW Anderson, JW Erickson, E Appella, D Xie
BIOCHEMISTRY, 36, 33, 10117, 10124, 1997年08月, ［査読有り］
英語, 研究論文（学術雑誌）

Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner
M Fiscella, HL Zhang, SJ Fan, K Sakaguchi, SF Shen, WE Mercer, GF VandeWoude, PM OConnor, E Appella
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 94, 12, 6048, 6053, 1997年06月, ［査読有り］
英語, 研究論文（学術雑誌）

Potent immunogenic short linear peptide constructs composed of B cell epitopes and Pan DR T Helper Epitopes (PADRE) for antibody responses in vivo
MF delGuercio, J Alexander, RT Kubo, T Arrhenius, A Maewal, E Appella, SL Hoffman, T Jones, D Valmori, K Sakaguchi, HM Grey, A Sette
VACCINE, 15, 4, 441, 448, 1997年03月, ［査読有り］
英語, 研究論文（学術雑誌）

Identification of subdominant CTL epitopes of the GP100 melanoma-associated tumor antigen by primary in vitro immunization with peptide-pulsed dendritic cells
Tsai, V, S Southwood, J Sidney, K Sakaguchi, Y Kawakami, E Appella, A Sette, E Celis
JOURNAL OF IMMUNOLOGY, 158, 4, 1796, 1802, 1997年02月, ［査読有り］
英語, 研究論文（学術雑誌）

Chemical synthesis of phosphorylated peptides of the carboxy-terminal domain of human p53 by a segment condensation method
H Sakamoto, H Kodama, Y Higashimoto, M Kondo, MS Lewis, CW Anderson, E Appella, K Sakaguchi
INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 48, 5, 429, 442, 1996年11月, ［査読有り］
英語, 研究論文（学術雑誌）

The proteolytic environment involved in MHC class II-restricted antigen presentation can be modulated by the p41 form of invariant chain
B Fineschi, K Sakaguchi, E Appella, J Miller
JOURNAL OF IMMUNOLOGY, 157, 8, 3211, 3215, 1996年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Chemical synthesis and applications of phosphopeptides
Sakaguchi Kazuyasu, Roller Peter P, Appella Ettore
Genetic engineering, 249, Springer, Boston, MA, 1996年, ［査読有り］

IDENTIFICATION OF A TYROSINASE EPITOPE RECOGNIZED BY HLA-A24-RESTRICTED, TUMOR-INFILTRATING LYMPHOCYTES
XQ KANG, Y KAWAKAMI, M ELGAMIL, RF WANG, K SAKAGUCHI, YANNELLI, JR, E APPELLA, SA ROSENBERG, PF ROBBINS
JOURNAL OF IMMUNOLOGY, 155, 3, 1343, 1348, 1995年08月, ［査読有り］
英語, 研究論文（学術雑誌）

CLONING OF A NEW GENE ENCODING AN ANTIGEN RECOGNIZED BY MELANOMA-SPECIFIC HLA-A24-RESTRICTED TUMOR-INFILTRATING LYMPHOCYTES
PF ROBBINS, M ELGAMIL, YF LI, SL TOPALIAN, L RIVOLTINI, K SAKAGUCHI, E APPELLA, Y KAWAKAMI, SA ROSENBERG
JOURNAL OF IMMUNOLOGY, 154, 11, 5944, 5950, 1995年06月, ［査読有り］
英語, 研究論文（学術雑誌）

BACKBONE DYNAMICS OF THE OLIGOMERIZATION DOMAIN OF P53 DETERMINED FROM N-15 NMR RELAXATION MEASUREMENTS
RT CLUBB, JG OMICHINSKI, K SAKAGUCHI, E APPELLA, AM GRONENBORN, GM CLORE
PROTEIN SCIENCE, 4, 5, 855, 862, 1995年05月, ［査読有り］
英語, 研究論文（学術雑誌）

REFINED SOLUTION STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF THE TUMOR-SUPPRESSOR P53
GM CLORE, J ERNST, R CLUBB, JG OMICHINSKI, WMP KENNEDY, K SAKAGUCHI, E APPELLA, AM GRONENBORN
NATURE STRUCTURAL BIOLOGY, 2, 4, 321, 333, 1995年04月, ［査読有り］
英語, 研究論文（学術雑誌）

RECOGNITION OF MULTIPLE EPITOPES IN THE HUMAN-MELANOMA ANTIGEN GP100 BY TUMOR-INFILTRATING T-LYMPHOCYTES ASSOCIATED WITH IN-VIVO TUMOR-REGRESSION
Y KAWAKAMI, S ELIYAHU, C JENNINGS, K SAKAGUCHI, XQ KANG, S SOUTHWOOD, PF ROBBINS, A SETTE, E APPELLA, SA ROSENBERG
JOURNAL OF IMMUNOLOGY, 154, 8, 3961, 3968, 1995年04月, ［査読有り］
英語, 研究論文（学術雑誌）

INDUCTION OF TUMOR-REACTIVE CTL FROM PERIPHERAL-BLOOD AND TUMOR-INFILTRATING LYMPHOCYTES OF MELANOMA PATIENTS BY IN-VITRO STIMULATION WITH AN IMMUNODOMINANT PEPTIDE OF THE HUMAN-MELANOMA ANTIGEN MART-1
L RIVOLTINI, Y KAWAKAMI, K SAKAGUCHI, S SOUTHWOOD, A SETTE, PF ROBBINS, FM MARINCOLA, ML SALGALLER, YR YANNELLI, E APPELLA, SA ROSENBERG
JOURNAL OF IMMUNOLOGY, 154, 5, 2257, 2265, 1995年03月, ［査読有り］
英語, 研究論文（学術雑誌）

THE HUMAN DNA-ACTIVATED PROTEIN-KINASE, DNA-PK, IS ACTIVATED BY DNA BREAKS AND PHOSPHORYLATES NUCLEAR DNA-BINDING PROTEIN SUBSTRATES ON SERINES AND THREONINES FOLLOWING BY GLUTAMINE
CW ANDERSON, MA CONNELLY, H ZHANG, JD SIPLEY, SP LEESMILLER, K SAKAGUCHI, SJ ULLRICH, SP JACKSON, E APPELLA
JOURNAL OF PROTEIN CHEMISTRY, 13, 5, 500, 501, 1994年07月, ［査読有り］
英語, 研究論文（学術雑誌）

STRUCTURE AND POSTTRANSLATIONAL MODIFICATION OF THE HUMAN P53 PROTEIN
E APPELLA, M FISCELLA, N ZAMBRANO, SJ ULLRICH, K SAKAGUCHI, H SAKAMOTO, MS LEWIS, D LIN, WE MERCER, CW ANDERSON
JOURNAL OF PROTEIN CHEMISTRY, 13, 5, 499, 500, 1994年07月, ［査読有り］
英語, 研究論文（学術雑誌）

IDENTIFICATION OF THE IMMUNODOMINANT PEPTIDES OF THE MART-1 HUMAN-MELANOMA ANTIGEN RECOGNIZED BY THE MAJORITY OF HLA-A2-RESTRICTED TUMOR-INFILTRATING LYMPHOCYTES
Y KAWAKAMI, S ELIYAHU, K SAKAGUCHI, PF ROBBINS, L RIVOLTINI, YANNELLI, JR, E APPELLA, SA ROSENBERG
JOURNAL OF EXPERIMENTAL MEDICINE, 180, 1, 347, 352, 1994年07月, ［査読有り］
英語

IDENTIFICATION OF A HUMAN-MELANOMA ANTIGEN RECOGNIZED BY TUMOR-INFILTRATING LYMPHOCYTES ASSOCIATED WITH IN-VIVO TUMOR REJECTION
Y KAWAKAMI, S ELIYAHU, CH DELGADO, PF ROBBINS, K SAKAGUCHI, E APPELLA, YANNELLI, JR, GJ ADEMA, T MIKI, SA ROSENBERG
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 91, 14, 6458, 6462, 1994年07月, ［査読有り］
英語, 研究論文（学術雑誌）

HIGH-RESOLUTION STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTIDIMENSIONAL NMR
GM CLORE, JG OMICHINSKI, K SAKAGUCHI, N ZAMBRANO, H SAKAMOTO, E APPELLA, AM GRONENBORN
SCIENCE, 265, 5170, 386, 391, 1994年07月, ［査読有り］
英語, 研究論文（学術雑誌）

STRUCTURAL ESSENTIALS OF SER-1 IN TETHERED PEPTIDE LIGAND OF HUMAN THROMBIN RECEPTOR FOR PHOSPHOINOSITIDE HYDROLYSIS
K SAKAGUCHI, H KODAMA, Y OGINO, T COSTA, T NOSE, Y SHIMOHIGASHI
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 67, 6, 1659, 1663, 1994年06月, ［査読有り］
英語, 研究論文（学術雑誌）

MOLECULAR-INTERACTIONS BETWEEN INTERFERON CONSENSUS SEQUENCE BINDING-PROTEIN AND MEMBERS OF THE INTERFERON REGULATORY FACTOR FAMILY
C BOVOLENTA, PH DRIGGERS, MS MARKS, JA MEDIN, AD POLITIS, SN VOGEL, DE LEVY, K SAKAGUCHI, E APPELLA, JE COLIGAN, K OZATO
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 91, 11, 5046, 5050, 1994年05月, ［査読有り］
英語, 研究論文（学術雑誌）

CD45 REGULATION OF TYROSINE PHOSPHORYLATION AND ENZYME-ACTIVITY OF SRC FAMILY KINASES
CM BURNS, K SAKAGUCHI, E APPELLA, JD ASHWELL
JOURNAL OF BIOLOGICAL CHEMISTRY, 269, 18, 13594, 13600, 1994年05月, ［査読有り］
英語, 研究論文（学術雑誌）

DEFINITION OF SPECIFIC PEPTIDE MOTIFS FOR 4 MAJOR HLA-A ALLELES
RT KUBO, A SETTE, HM GREY, E APPELLA, K SAKAGUCHI, NZ ZHU, D ARNOTT, N SHERMAN, J SHABANOWITZ, H MICHEL, WM BODNAR, TA DAVIS, DF HUNT
JOURNAL OF IMMUNOLOGY, 152, 8, 3913, 3924, 1994年04月, ［査読有り］
英語, 研究論文（学術雑誌）

NATURALLY PROCESSED PEPTIDES LONGER THAN 9 AMINO-ACID-RESIDUES BIND TO THE CLASS-I MHC MOLECULE HLA-A2.1 WITH HIGH-AFFINITY AND IN DIFFERENT CONFORMATIONS
Y CHEN, J SIDNEY, S SOUTHWOOD, AL COX, K SAKAGUCHI, RA HENDERSON, E APPELLA, DF HUNT, A SETTE, VH ENGELHARD
JOURNAL OF IMMUNOLOGY, 152, 6, 2874, 2881, 1994年03月, ［査読有り］
英語, 研究論文（学術雑誌）

THE PERIPHERAL SUBUNIT-BINDING DOMAIN OF THE DIHYDROLIPOYL ACETYLTRANSFERASE COMPONENT OF THE PYRUVATE-DEHYDROGENASE COMPLEX OF BACILLUS-STEAROTHERMOPHILUS - PREPARATION AND CHARACTERIZATION OF ITS BINDING TO THE DIHYDROLIPOYL DEHYDROGENASE COMPONENT
DS HIPPS, LC PACKMAN, MD ALLEN, C FULLER, K SAKAGUCHI, E APPELLA, RN PERHAM
BIOCHEMICAL JOURNAL, 297, 1, 137, 143, 1994年01月, ［査読有り］
英語, 研究論文（学術雑誌）

DIRECT IDENTIFICATION OF AN ENDOGENOUS PEPTIDE RECOGNIZED BY MULTIPLE HLA-A2.1-SPECIFIC CYTOTOXIC T-CELLS
RA HENDERSON, AL COX, K SAKAGUCHI, E APPELLA, J SHABANOWITZ, DF HUNT, VH ENGELHARD
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 90, 21, 10275, 10279, 1993年11月, ［査読有り］
英語, 研究論文（学術雑誌）

CHARACTERISTICS OF ENDOGENOUS PEPTIDES ELUTED FROM THE CLASS-I MHC MOLECULE HLA-B7 DETERMINED BY MASS-SPECTROMETRY AND COMPUTER MODELING
EL HUCZKO, WM BODNAR, D BENJAMIN, K SAKAGUCHI, NZ ZHU, J SHABANOWITZ, RA HENDERSON, E APPELLA, DF HUNT, VH ENGELHARD
JOURNAL OF IMMUNOLOGY, 151, 5, 2572, 2587, 1993年09月, ［査読有り］
英語, 研究論文（学術雑誌）

2-DIMENSIONAL NUCLEAR-MAGNETIC-RESONANCE ANALYSIS OF A LABELED PEPTIDE BOUND TO A CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX MOLECULE
PC DRISCOLL, JD ALTMAN, JJ BONIFACE, K SAKAGUCHI, PA REAY, JG OMICHINSKI, E APPELLA, MM DAVIS
JOURNAL OF MOLECULAR BIOLOGY, 232, 2, 342, 350, 1993年07月, ［査読有り］
英語, 研究論文（学術雑誌）

PHOSPHORYLATION AT SER-15 AND SER-392 IN MUTANT P53-MOLECULES FROM HUMAN TUMORS IS ALTERED COMPARED TO WILD-TYPE P53
SJ ULLRICH, K SAKAGUCHI, SP LEESMILLER, M FISCELLA, WE MERCER, CW ANDERSON, E APPELLA
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 90, 13, 5954, 5958, 1993年07月, ［査読有り］
英語, 研究論文（学術雑誌）

IDENTIFICATION OF A BINDING-SITE FOR THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NUCLEOCAPSID PROTEIN
K SAKAGUCHI, N ZAMBRANO, ET BALDWIN, BA SHAPIRO, JW ERICKSON, JG OMICHINSKI, GM CLORE, AM GRONENBORN, E APPELLA
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 90, 11, 5219, 5223, 1993年06月, ［査読有り］
英語, 研究論文（学術雑誌）

THE EZRIN-LIKE FAMILY OF TYROSINE KINASE SUBSTRATES - RECEPTOR-SPECIFIC PATTERN OF TYROSINE PHOSPHORYLATION AND RELATIONSHIP TO MALIGNANT TRANSFORMATION
F FAZIOLI, WT WONG, SJ ULLRICH, K SAKAGUCHI, E APPELLA, PP DIFIORE
ONCOGENE, 8, 5, 1335, 1345, 1993年05月, ［査読有り］
英語, 研究論文（学術雑誌）

SEQUENCE OF A CDNA-ENCODING BOVINE APOLIPOPROTEIN-H
B GAO, M VIRMANI, E ROMM, E LAZARWESLEY, K SAKAGUCHI, E APPELLA, G KUNOS, L TAKACS
GENE, 126, 2, 287, 288, 1993年04月, ［査読有り］
英語

THE HIGH-RESOLUTION STRUCTURE OF THE PERIPHERAL SUBUNIT-BINDING DOMAIN OF DIHYDROLIPOAMIDE ACETYLTRANSFERASE FROM THE PYRUVATE-DEHYDROGENASE MULTIENZYME COMPLEX OF BACILLUS-STEAROTHERMOPHILUS
YN KALIA, SM BROCKLEHURST, DS HIPPS, E APPELLA, K SAKAGUCHI, RN PERHAM
JOURNAL OF MOLECULAR BIOLOGY, 230, 1, 323, 341, 1993年03月, ［査読有り］
英語, 研究論文（学術雑誌）

OPTIMUM CROSS-LINKING SPACER LENGTH OF DIMERIC NEUROKININ-B ANALOGS FOR INTERACTION WITH NK-1 TACHYKININ RECEPTORS
H MATSUMOTO, Y SHIMOHIGASHI, Y TAKANO, K SAKAGUCHI, H KAMIYA, M OHNO
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 66, 1, 196, 204, 1993年01月, ［査読有り］
英語, 研究論文（学術雑誌）

INVARIANT CHAIN PEPTIDES IN MOST HLA-DR MOLECULES OF AN ANTIGEN-PROCESSING MUTANT
A SETTE, S CEMAN, RT KUBO, K SAKAGUCHI, E APPELLA, DF HUNT, TA DAVIS, H MICHEL, J SHABANOWITZ, R RUDERSDORF, HM GREY, R DEMARS
SCIENCE, 258, 5089, 1801, 1804, 1992年12月, ［査読有り］
英語, 研究論文（学術雑誌）

HUMAN DNA-ACTIVATED PROTEIN-KINASE PHOSPHORYLATES SERINE-15 AND SERINE-37 IN THE AMINO-TERMINAL TRANSACTIVATION DOMAIN OF HUMAN P53
SP LEESMILLER, K SAKAGUCHI, SJ ULLRICH, E APPELLA, CW ANDERSON
MOLECULAR AND CELLULAR BIOLOGY, 12, 11, 5041, 5049, 1992年11月, ［査読有り］
英語, 研究論文（学術雑誌）

THYMOCYTE COSTIMULATING ANTIGEN IS CD26 (DIPEPTIDYLPEPTIDASE-IV) - COSTIMULATION OF GRANULOCYTE, MACROPHAGE, AND T-LINEAGE CELL-PROLIFERATION VIA CD26
LA BRISTOL, K SAKAGUCHI, E APPELLA, D DOYLE, L TAKACS
JOURNAL OF IMMUNOLOGY, 149, 2, 367, 372, 1992年07月, ［査読有り］
英語, 研究論文（学術雑誌）

PEPTIDES PRESENTED TO THE IMMUNE-SYSTEM BY THE MURINE CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX MOLECULE-I-A(D)
DF HUNT, H MICHEL, TA DICKINSON, J SHABANOWITZ, AL COX, K SAKAGUCHI, E APPELLA, HM GREY, A SETTE
SCIENCE, 256, 5065, 1817, 1820, 1992年06月, ［査読有り］
英語, 研究論文（学術雑誌）

INTERNALIZATION OF THE UROKINASE-PLASMINOGEN ACTIVATOR INHIBITOR TYPE-1 COMPLEX IS MEDIATED BY THE UROKINASE RECEPTOR
D OLSON, J POLLANEN, G HOYERHANSEN, E RONNE, K SAKAGUCHI, TC WUN, E APPELLA, K DANO, F BLASI
JOURNAL OF BIOLOGICAL CHEMISTRY, 267, 13, 9129, 9133, 1992年05月, ［査読有り］
英語, 研究論文（学術雑誌）

3-DIMENSIONAL SOLUTION STRUCTURE OF THE E3-BINDING DOMAIN OF THE DIHYDROLIPOAMIDE SUCCINYL TRANSFERASE CORE FROM THE 2-OXOGLUTARATE DEHYDROGENASE MULTIENZYME COMPLEX OF ESCHERICHIA-COLI
MA ROBIEN, GM CLORE, JG OMICHINSKI, RN PERHAM, E APPELLA, K SAKAGUCHI, AM GRONENBORN
BIOCHEMISTRY, 31, 13, 3463, 3471, 1992年04月, ［査読有り］
英語, 研究論文（学術雑誌）

RECEPTOR INTERACTIONS OF SYNTHETIC MORPHICEPTIN ANALOGS CONTAINING PHENYLALANINE HOMOLOGS IN POSITION-4
K SAKAGUCHI, T COSTA, H SAKAMOTO, Y SHIMOHIGASHI
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 65, 4, 1052, 1056, 1992年04月, ［査読有り］
英語, 研究論文（学術雑誌）

HLA-A2.1-ASSOCIATED PEPTIDES FROM A MUTANT-CELL LINE - A 2ND PATHWAY OF ANTIGEN PRESENTATION
RA HENDERSON, H MICHEL, K SAKAGUCHI, J SHABANOWITZ, E APPELLA, DF HUNT, VH ENGELHARD
SCIENCE, 255, 5049, 1264, 1266, 1992年03月, ［査読有り］
英語, 研究論文（学術雑誌）

CHARACTERIZATION OF PEPTIDES BOUND TO THE CLASS-I MHC MOLECULE HLA-A2.1 BY MASS-SPECTROMETRY
DF HUNT, RA HENDERSON, J SHABANOWITZ, K SAKAGUCHI, H MICHEL, N SEVILIR, AL COX, E APPELLA, VH ENGELHARD
SCIENCE, 255, 5049, 1261, 1263, 1992年03月, ［査読有り］
英語, 研究論文（学術雑誌）

High-resolution structure of a double zinc finger from the human enhancer binding protein MPB-1 in solution
Omichinski James G, Sakaguchi Kazuyasu, Clore G Marius, Gronenborn Angela M, Appella Ettore
Journal of Protein Chemistry, 11, 4, 408, Springer, 1992年, ［査読有り］

SPECIFIC DNA-BINDING TO A MAJOR HISTOCOMPATIBILITY COMPLEX ENHANCER SEQUENCE BY A SYNTHETIC 57-RESIDUE DOUBLE ZINC FINGER PEPTIDE FROM A HUMAN ENHANCER BINDING-PROTEIN
K SAKAGUCHI, E APPELLA, JG OMICHINSKI, GM CLORE, AM GRONENBORN
JOURNAL OF BIOLOGICAL CHEMISTRY, 266, 11, 7306, 7311, 1991年04月, ［査読有り］
英語, 研究論文（学術雑誌）

HIGH-RESOLUTION 3-DIMENSIONAL STRUCTURE OF A SINGLE ZINC FINGER FROM A HUMAN ENHANCER BINDING-PROTEIN IN SOLUTION
JG OMICHINSKI, GM CLORE, E APPELLA, K SAKAGUCHI, AM GRONENBORN
BIOCHEMISTRY, 29, 40, 9324, 9334, 1990年10月, ［査読有り］
英語, 研究論文（学術雑誌）

Opioid Activities of Morphiceptin Analogs Derived from Human $β$-Casein
Sakaguchi Kazuyasu, Sakamoto Hiroshi, Tsubaki Yoshiko, Waki Michinori, Costa Tommaso, Shimohigashi Yasuyuki
Bulletin of the Chemical Society of Japan, 63, 6, 1753, 1757, 1990年06月, ［査読有り］
英語, 研究論文（学術雑誌）

CHARACTERIZATION OF TACHYKININ RECEPTORS IN ENDOTHELIAL-CELLS OF PORCINE ARTERY
R SAITO, H KONISHI, Y TAKANO, S NONAKA, K SAKAGUCHI, Y SHIMOHIGASHI, HO KAMIYA
NEUROSCIENCE LETTERS, 110, 3, 337, 342, 1990年03月, ［査読有り］
英語, 研究論文（学術雑誌）

Opioid activities of morphiceptin-like peptides latent in various natural proteins.
Shimohigashi Y, Sakaguchi K, Sakamoto H, Waki M, Costa T
Peptide research, 3, 5, 216, 1990年, ［査読有り］

Design and synthesis of dimeric analogs of neurokinin A and B: effect of dimerization of COOH-terminal heptapeptides on receptor selection.
Sakaguchi K, Kodama H, Matsumoto H, Yoshida M, Takano Y, Kamiya H, Waki M, Shimohigashi Y
Peptide research, 2, 5, 345, 1989年, ［査読有り］

STUDIES OF PEPTIDE ANTIBIOTICS .48. CONFORMATIONALLY STABILIZED GRAMICIDIN S-ANALOG CONTAINING DEHYDROPHENYLALANINE IN PLACE OF D-PHENYLALANINE4,4' - SYNTHESIS AND ANTIMICROBIAL ACTIVITY
S IMAZU, Y SHIMOHIGASHI, H KODAMA, K SAKAGUCHI, M WAKI, T KATO, N IZUMIYA
INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 32, 4, 298, 306, 1988年10月, ［査読有り］
英語, 研究論文（学術雑誌）

DIMERIZATION OF NEUROKININ-A AND NEUROKININ-B COOH-TERMINAL HEPTAPEPTIDE FRAGMENTS ENHANCED THE SELECTIVITY FOR TACHYKININ RECEPTOR SUBTYPES
H KODAMA, Y SHIMOHIGASHI, K SAKAGUCHI, M WAKI, Y TAKANO, A YAMADA, Y HATAE, H KAMIYA
EUROPEAN JOURNAL OF PHARMACOLOGY, 151, 2, 317, 320, 1988年07月, ［査読有り］
英語

BIOLOGICAL EVALUATION OF DIMERIC ANALOGS OF TACHYKININ PEPTIDES
Y SHIMOHIGASHI, H KODAMA, K SAKAGUCHI, T KATO, M WAKI, H KAMIYA, A YAMADA, Y HIGUCHI, Y TAKANO
REGULATORY PEPTIDES, 22, 1-2, 172, 172, 1988年07月, ［査読有り］
英語

自然免疫を担うインターフェロン経路における「転写記憶」の発見とその制御機構解明　　　　　　　　　　　　　　　
鎌田瑠泉, 坂口和靖, 尾里啓子, 実験医学, 37, 3, 415, 418, 2019年02月, ［招待有り］
日本語

EVOLUTION OF THE TETRAMERIZATION DOMAIN IN TUMOR SUPPRESSOR PROTEIN P53
N. Nakagawa, J. Wada, R. Kamada, T. Nomura, T. Imagawa, K. Sakaguchi, JOURNAL OF PEPTIDE SCIENCE, 22, S22, S22, 2016年09月
英語, 研究発表ペーパー・要旨（国際会議）

ARGININE METHYLATION OF TETRAMERIZATION DOMAIN DESTABILIZES THE TETRAMERIC STRUCTURE OF TUMOR SUPPRESSOR PROTEIN P53
R. Kamada, N. Nakagawa, J. Wada, K. Sakaguchi, JOURNAL OF PEPTIDE SCIENCE, 22, S128, S128, 2016年09月
英語, 研究発表ペーパー・要旨（国際会議）

THE EFFECTS OF P53 TETRAMERIZATION DOMAIN MUTANTS FOUND IN LI-FRAUMENI SYNDROME ON HETERO-OLIGOMER FORMATION AND TRANSCRIPTIONAL ACTIVITY
Y. Toguchi, M. Kanno, R. Kamada, T. Imagawa, K. Sakaguchi, JOURNAL OF PEPTIDE SCIENCE, 22, S86, S88, 2016年09月
英語, 研究発表ペーパー・要旨（国際会議）

癌原遺伝子産物プロテインホスファターゼによる細胞癌化機構と阻害剤開発
小笠原紗里, 鎌田瑠泉, 坂口和靖, 化学工業, 67, 10, 61, 66, 2016年, ［招待有り］
小峰工業出版, 日本語, 記事・総説・解説・論説等（学術雑誌）

The PPM1D encoded phosphatase Wip1 is a novel oncogene and potential therapeutic target in neuroblastoma and medulloblastoma
Jelena Miloseyic, Diana Treis, Malin Wickstrom, Susanne Fransson, Nina Eissler, Baldur Syeinbjornsson, Ninib Baryawno, Keiji Tanino, Galina Selivanova, Kazuyasu Sakaguchi, Tommy Martinsson, John Inge Johnsen, Per Kogner, CANCER RESEARCH, 75, 2015年08月
英語, 研究発表ペーパー・要旨（国際会議）

多量体化と配向化を基盤としたバイオミネラリゼーションペプチドによる銀ナノ構造制御
坂口達也, 峯健太, 工藤風樹, 鎌田瑠泉, 坂口和靖, 日本生化学会大会(Web), 88th, 2015年

がん原遺伝子産物PPM1Dの細胞がん化機構および創薬を指向した阻害剤
鎌田瑠泉, 中馬吉郎, 小境夕紀, 坂口和靖, 生化学, 87, 531, 538, 2015年, ［査読有り］, ［招待有り］
日本語, 記事・総説・解説・論説等（学術雑誌）

Analysis of Substrate Preference of Human PPM1 Type Ser/Thr Phosphatases
SHIRAHATA Yukiko, CHUMAN Yoshiro, IWAMURO Ryo, JANAIRO Jose isagani B., KIYOTA Yuhei, YAGI Hiroaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2012, 403, 404, 2013年03月01日
英語

Silver Nanocrystals Formed by Oligomeric Biomineralization Peptide via p53 Tetramerization Domain
SAKAGUCHI Tatsuya, JANAIRO Jose Isagani B., CHUMAN Yoshiro, HARA Kenji, FUKUOKA Atsushi, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2012, 57, 58, 2013年03月01日
英語

p53四量体形成ドメインを介したバイオミネラリゼーションペプチドの立体配置による銀ナノ結晶形成
坂口達也, JANAIRO Jose Isagani, 中馬吉郎, 原賢二, 福岡淳, 坂口和靖, 日本化学会講演予稿集, 93rd, 3, 2013年

Probing the Differential Phosphothreonine and Metal Selectivity of Human PPM1 Phosphatase Family
JANAIRO Jose Isagani, IWAMURO Ryo, KAYA Shunji, YAGI Hiroaki, CHUMAN Yoshiro, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 245, 246, 2012年03月01日
英語

Development of Screening System for Tumor Suppressor p53 Activators and Inhibitors
UESUGI Ken-ichiro, OSHIMA Shogo, TANAKA Aya, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 403, 404, 2012年03月01日
英語

Analysis of Interaction between p53-Inducible Protein Phosphatase PPM1D and Nucleolar Protein
KOZAKAI Yuuki, YAGI Hiroaki, TEDUKA Youhei, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 43, 44, 2012年03月01日
英語

Formation of Functionalized Nanowires Using Structure-Controllable Amyloid Peptide
SAKAI Hiroki, WATANABE Ken, CHUMAN Yoshiro, MASUDA Takuya, UOSAKI Kohei, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 23, 24, 2012年03月01日
英語

Repression of p53 Transcriptional Activity by Inhibitory Peptide via Heterotetramerization
WADA Junya, KAMADA Rui, CHUMAN Yoshiro, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 301, 302, 2012年03月01日
英語

Nanostructure Formed by Biomineralization Peptide Oligomerized via p53 Tetramerization
SAKAGUCHI Tatsuya, KAMADA Rui, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 345, 346, 2012年03月01日
英語

Involvement of PPM1D Specific Pro-Loop in Regulatory Mechanism of PPM1D Phosphatase Activity
YAGI Hiroaki, CHUMAN Yoshiro, KOZAKAI Yuuki, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2011, 373, 374, 2012年03月01日
英語

p53四量体形成ドメインを介した多量体化バイオミネラリゼーションペプチドによる銀粒子形成
坂口達也, 鎌田瑠泉, 中馬吉郎, 坂口和靖, 日本化学会講演予稿集, 92nd, 3, 2012年

Formation Process and Solvent-Dependent Structure of a Polyproline Self-Assembled Monolayer on a Gold Surface
Ying Han, Hidenori Noguchi, Kazuyasu Sakaguchi, Kohei Uosaki, LANGMUIR, 27, 19, 11951, 11957, 2011年10月
英語

Effect of Peptide Oligomerization Mediated by p53 Tetramerization Domain on Biomineralization Activity
SAKAGUCHI Tatsuya, KAMADA Rui, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2010, 269, 269, 2011年03月01日
英語

Controlling Initiation Position of Amyloid Nanowire Using a Gold Nanoparticle
SAKAI Hiroki, WATANABE Ken, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2010, 276, 276, 2011年03月01日
英語

Dephosphorylation of Nucleolar Protein Nucleophosmin by Protein Phosphatase PPM1D
KOZAKAI Yuuki, YAGI Hiroaki, CHUMAN Yoshiro, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2010, 92, 92, 2011年03月01日
英語

Repression of p53 transcriptional activity by p53 tetramerization domain peptide containing PTD and NLS domain
WADA Junya, KAMADA Rui, CHUMAN Yoshiro, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2010, 95, 95, 2011年03月01日
英語

Low Threshold of Destabilization for Loss of Tumor Suppressor Activity of p53 : A Quantitative Analysis of p53 Tetramerization Domain Mutants
KAMADA Rui, NOMURA Takao, CHUMAN Yoshiro, IMAGAWA Toshiaki, ANDERSON Carl W., SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2010, 149, 149, 2011年03月01日
英語

p53四量体形成ドメインを介した空間配向制御によるバイオミネラリゼーションの活性増強と構造制御
坂口達也, 鎌田瑠泉, 野村尚生, 中馬吉郎, 今川敏明, 坂口和靖, 日本化学会講演予稿集, 90th, 3, 2010年

p53四量体形成ドメインとバイオミネラリゼーションペプチドの融合による銀粒子の構造制御
坂口達也, 鎌田瑠泉, 野村尚生, 中馬吉郎, 今川敏明, 坂口和靖, 日本化学会北海道支部夏季研究発表会講演要旨集, 2010, 2010年

赤外分光法によるペプチド固相合成過程におけるペプチド構造のその場追跡
野口秀典, 野口秀典, 安達龍彦, 坂口和靖, 魚崎浩平, 魚崎浩平, 日本化学会講演予稿集, 90th, 3, 2010年

がん抑制タンパク質p53の四量体形成　　　　　　　　　　　　　　　
鎌田瑠泉, 坂口和靖, 生化学, 82, 7, 484, 493, 2010年, ［査読有り］, ［招待有り］
日本語, 記事・総説・解説・論説等（学術雑誌）

Extremely Long Nanofibrils of Functionalized Structure-Controlled-Amyloid Peptides Derived from Transthyretin
SAKAI Hiroki, ASANOMI Yuya, KOBAYASHI Yumiko, CHEN Xinjiang, CHUMAN Yoshiro, MASUDA Takuya, UOSAKI Kohei, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2008, 465, 466, 2009年03月01日
英語

PPM1D430, a Novel Alternative Splicing Variant of the Human PPM1D
CHUMAN Yoshiro, KURIHASHI Wataru, MIZUKAMI Yohei, NASHIMOTO Takehiro, YAGI Hiroaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2008, 531, 532, 2009年03月01日
英語

Thermal Stability of p53 Tetramerization Domain Peptides Derived from Various Species
NOMURA Takao, KAMADA Rui, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2008, 35, 36, 2009年03月01日
英語

Identification of Novel Chemical Inhibitors for p53-Inducible Protein Phosphatase PPM1D
YAGI Hiroaki, CHUMAN Yoshiro, YOSHIMURA Fumihiko, TANINO Keiji, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2008, 385, 386, 2009年03月01日
英語

Structural Photomodulation of Peptides with Azobenzoate Derivative in Peptide Backbone
KAMADA Rui, FUKUDA Kouichiro, MIYAZAKI Hiromitsu, NOMURA Takao, CHUMAN Yoshiro, IMAGAWA Toshiaki, TANINO Keiji, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2007, 447, 448, 2008年03月01日
英語

Correlation between Expression Level in E. coli and Stability of Oligomeric Structure of the p53 Tetramerization Domain
MIYAZAKI Hiromitsu, KAMADA Rui, NOMURA Takao, CHUMAN Yoshiro, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2007, 317, 318, 2008年03月01日
英語

Screening of Peptides to Stabilize the p53 Tetramer Formation from Phage Displayed Library
NOMURA Takao, KAMADA Rui, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2007, 315, 316, 2008年03月01日
英語

Correlation of Oligomeric Structure and Transcriptional Activity of p53 Mutants
KAMADA Rui, TERAI Tomoko, NOMURA Takao, CHUMAN Yoshiro, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2007, 69, 70, 2008年03月01日
英語

Function of Basic Loop in Substrate Recognition of Protein Phosphatase PPM1D
CHUMAN Yoshiro, FUKUDA Tomohiko, YAGI Hiroaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2006, 17, 18, 2007年03月01日
英語

Stabilization of p53 Tetrameric Structure by Polyol Compounds
NOMURA Takao, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2006, 131, 132, 2007年03月01日
英語

Thermodynamic Stability of Mutant p53 Tetramerization Domain Peptides Associated with Human Tumors
KAMADA Rui, TERAI Tomoko, NOMURA Takao, CHUMAN Yoshiro, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2006, 293, 294, 2007年03月01日
英語

Effects of Tumor-Associated mutations in the p53 tetramerization domain on oligomerization state and transcriptional activity
R. Kamada, T. Terai, T. Nomura, Y. Chuman, T. Imagawa, K. Sakaguchi, BIOPOLYMERS, 88, 4, 626, 626, 2007年
英語, 研究発表ペーパー・要旨（国際会議）

Effects of Mutation in the β-Strand Region of p53 Tetramerizaion Domain on Oligomeric Structure and Stability
KAMADA Rui, TERAI Tomoko, NOMURA Takao, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2005, 267, 270, 2006年03月01日
英語

Fibrillar and Granular Aggregation of G334V Mutant p53 Tetramer Peptide
ASANOMI Yuya, HIGASHIMOTO Yuichiro, CHUMAN Yoshiro, KAYA Shunji, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2005, 397, 398, 2006年03月01日
英語

Analysis of Stabilization by Substitution of Cyclohexylalanine for Phe341 in Hydrophobic Core of p53 Tetramer
NOMURA Takao, SAKAMOTO Koichi, KASAI Yusuke, CHUMAN Yoshiro, ISHIMORI Koichiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2005, 399, 402, 2006年03月01日
英語

Characterization and Application of Monoclonal Antibody Specific for Ser(P)-Gln Phosphorylation Motif
IIZUKA Kanako, CHUMAN Yoshiro, HONDA Takeshi, ONOUE Hitoshi, SHIMOHIGASHI Yasuyuki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2005, 499, 502, 2006年03月01日
英語

Substrate Recognition of PP2C Family Phosphatase PPM1D
FUKUDA Tomohiko, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2005, 503, 504, 2006年03月01日
英語

New evidence for ATP binding induced catalytic subunit interactions in pig kidney Na/K-ATPase.　　　　　　　　　　　　　　　
Tanoue, K, Kaya, S, Hayashi, Y, Abe, K, Imagawa, T, Taniguchi, K, Sakaguchi, K, Journal of Biochemistry, 140, 599-607, 2006年
英語, 速報，短報，研究ノート等（学術雑誌）

The third Na+ binding pocket is located near the amino acid residues Thr-774 and Gln-923 in rat Na,K-ATPase.
T Imagawa, T Yamamoto, S Kaya, K Sakaguchi, K Taniguchi, JOURNAL OF GENERAL PHYSIOLOGY, 126, 1, 19A, 19A, 2005年07月
英語, 研究発表ペーパー・要旨（国際会議）

High and low affinity ATP effects on both NaE1 and KE2 of Na/K-ATPase studied by substrate analogues.
K Tanoue, S Kaya, K Abe, T Imagawa, Y Hayashi, K Sakaguchi, K Taniguchi, JOURNAL OF GENERAL PHYSIOLOGY, 126, 1, 41A, 42A, 2005年07月
英語, 研究発表ペーパー・要旨（国際会議）

Interaction region between catalytic subunits during ATP hydrolysis in oligomeric Na/K-ATPase.
S Kaya, T Imagawa, K Sakaguchi, K Taniguchi, JOURNAL OF GENERAL PHYSIOLOGY, 126, 1, 37A, 38A, 2005年07月
英語, 研究発表ペーパー・要旨（国際会議）

K+-induced change in oligomeric assembly of C12E8-solubilized pig gastric H/K-ATPase observed by single-molecule detection techniques.
K Abe, S Kaya, K Sakaguchi, T Imagawa, Y Hayashi, K Taniguchi, JOURNAL OF GENERAL PHYSIOLOGY, 126, 1, 34A, 35A, 2005年07月
英語, 研究発表ペーパー・要旨（国際会議）

Effects of Mutation and Modification on Tetramer Formation of Tumor Suppressor Protein p53
SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2004, 17, 20, 2005年03月01日
英語

DNA Binding of Peptide with Single Helix-hairpin-Helix Motif
YAMAMOTO Tetsuya, CHUMAN Yoshiro, KAYA Shunji, IMAGAWA Toshiaki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2004, 517, 520, 2005年03月01日
英語

Stability and Folding Mechanism for Trp-contaning Peptides of p53 Tetramerization Domain
KASAI Yusuke, KAYA Shunji, CHUMAN Yoshiro, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2004, 419, 422, 2005年03月01日
英語

Characteraization of Superstable Variant Peptide of Human p53 Tetramerization Domain
NOMURA Takao, ITO Issaku, CHUMAN Yoshiro, SHIMOHIGASHI Yasuyuki, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2004, 423, 426, 2005年03月01日
英語

δ‐オピオイド受容体のチオールジスルフィド交換反応による特異的親和標識
磯崎要, 深堀英彦, 岡田一志, 白須直人, 本田健, 野瀬健, 坂口和靖, COSTA T, 下東康幸, 生化学, 76, 3, 319, 2004年03月25日
日本語

概日リズムペースメーカーホルモン・PDFの局在部位解析を目的とした部位特異的抗体の作製
本田健, 中馬吉郎, 浅井大輔, 野瀬健, 坂口和靖, 尾上均, 冨永佳也, 下東康幸, 下東美樹, 生化学, 76, 3, 318, 2004年03月25日
日本語

Destabilization and Oligomer Formation of p53 Tetramer Mutant Associated with Human Lung Carcinoma
HIGASHIMOTO Yuichiro, ASANOMI Yuya, LEWIS Marc S., APPELLA Ettore, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2003, 17, 20, 2004年03月01日
英語

Localization Profiles of Circadian Neuropeptide PDF in Brain of the Cricket Gryllus Bimaculatus
HONDA Takeshi, CHUMAN Yoshiro, MATSUSHIMA Ayami, ASAI Daisuke, SUMIDA Kazunori, NOSE Takeru, SAKAGUCHI Kazuyasu, ONOUE Hitoshi, TOMINAGA Yoshiya, SHIMOHIGASHI Yasuyuki, SHIMOHIGASHI Miki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2003, 81, 84, 2004年03月01日
英語

Mutant p53 Peptide Forms Amyloid under Physiological Condition
ASANOMI Yuya, HIGASHIMOTO Yuichiro, IMAGAWA Toshiaki, KAYA Shunji, APPELLA Ettore, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2003, 379, 382, 2004年03月01日
英語

Binding Specificity of HhH Motif Peptide that Recognizes DNA Structure
YAMAMOTO Tetsuya, IMAGAWA Toshiaki, KAYA Shunji, SAKAGUCHI Kazuyasu, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2003, 311, 314, 2004年03月01日
英語

Specific Affinity Labeling of Cys60 in the δ Opioid Receptor by Npys-containing Dynorphin A Analog
ISOZAKI Kaname, FUKAHORI Hidehiko, OKADA Kazushi, SHIRASU Naoto, HONDA Takeshi, NOSE Takeru, SAKAGUCHI Kazuyasu, COSTA Tommaso, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2003, 273, 276, 2004年03月01日
英語

Hetero-Oligomer Formation of Peptides Derived from Tetramerization Domain in Tumor Suppressor Protein p53
SAKAGUCHI Kazuyasu, MENO Takashi, ASANOMI Yuya, NOSE Takeru, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2003, 307, 310, 2004年03月01日
英語

Presence of two different ATP effects on both Na-bound and Rb-occluded Na/K-ATPase
K Taniguchi, K Tanoue, K Abe, S Kaya, T Imagawa, K Sakaguchi, BIOPHYSICAL JOURNAL, 86, 1, 192A, 192A, 2004年01月
英語, 研究発表ペーパー・要旨（国際会議）

Localization Profiles of the Precursor Protein of Circadian Rhythm Pacemaker Hormone PDF in Gryllus Brain
HONDA Takeshi, CHUMAN Yoshiro, MATSUSHIMA Ayami, ASAI Daisuke, NOSE Takeru, SAKAGUCHI Kazuyasu, ONOUE Hitoshi, TOMINAGA Yoshiya, SHIMOHIGASHI Yasuyuki, SHIMOHIGASHI Miki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2002, 433, 436, 2003年02月01日
英語

Phosphorylation Site-Specific Monoclonal Antibody Recognizing Ser(P)-Gln Sequence
SAKAGUCHI Kazuyasu, HONDA Takeshi, ONOUE Hitoshi, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2002, 439, 442, 2003年02月01日
英語

Specific Affinity Labeling of Delta Opioid Receptors via Thiol-Disulfide Exchange Reaction
FUKAHORI Hidehiko, SHIRASU Naoto, NOSE Takeru, SAKAGUCHI Kazuyasu, COSTA Tommaso, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2002, 257, 260, 2003年02月01日
英語

Structure-Activity Studies on the Nociceptin Peptidic Antagonist Ac-RYYRIK-NH_2
KAWANO Michiaki, HONDA Takeshi, OKADA Kazushi, CHUMAN Yoshiro, NOSE Takeru, SAKAGUCHI Kazuyasu, COSTA Tommaso, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2001, 185, 188, 2002年03月01日
英語

Structural Characterization of Phe Residues in the p53 Tetramerization Domain
SAKAGUCHI Kazuyasu, ITO Issaku, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2001, 135, 138, 2002年03月01日
英語

Characteristic Intra- and Intermolecular Interactions of Dipeptide-Chymotrypsin Complex as Specific Structural Essentials for Enzyme Inhibition
KOMADA Akira, YAMAUCHI Yasuko, IKESUE Koichi, FUJITA Tsugumi, NOSE Takeru, SAKAGUCHI Kazuyasu, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2001, 179, 182, 2002年03月01日
英語

ノシセプチンのアンタゴニスト・Ac‐RYYRIK‐NH₂のORL1受容体結合における構造活性相関
河野道昭, 本田健, 岡田一志, 中馬吉郎, 野瀬健, 坂口和靖, COSTA T, 下東康幸, 生化学, 73, 11, 1376, 2001年11月25日
日本語

鎮痛ペプチド・エンドモルフィン2Phe‐Phe残基の(F₅)Phe‐(F₅)Pheジペプチド置換による生物活性の変化
本田健, 岡田一志, 藤田亜美, 野瀬健, 坂口和靖, COSTA T, 下東康幸, 生化学, 73, 8, 945, 2001年08月25日
日本語

ORL1ノシセプチン受容体アンタゴニスト・Ac‐RYYRIK‐NH₂の構造活性相
河野道昭, 本田健, 岡田一志, 中馬吉郎, 野瀬健, 坂口和靖, COSTA T, 下東康幸, 生化学, 73, 8, 946, 2001年08月25日
日本語

ジペプチドH‐D‐Leu‐Phe‐NH‐Bzlのキモトリプシン阻害における疎水相互作用およびπ‐πスタッキング相互作用の重要性
駒田彰, 藤田亜美, 野瀬健, 坂口和靖, 下東康幸, 日本化学会講演予稿集, 79th, 2, 1027, 2001年03月15日
日本語

Preparation of the Antibody Which Discriminates the Conformations of Ligand-bound and Ligand-free Estrogen Receptors : Applications to the Quantitative Analysis of Ligand Bindings
ASAI Daisuke, KOIZUMI Osamu, MOHRI Shirou, NAKAI Makoto, YAKABE Yoshikuni, SAKAGUCHI Kazuyasu, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2000, 411, 412, 2001年03月01日
英語

Destabilization of Tumor Suppressor Protein p53 Tetramer by Oxidation of Met340
SAKAGUCHI Kazuyasu, ITO Issaku, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2000, 277, 280, 2001年03月01日
英語

Differential Receptor Binding Characteristics of the Phenylalanine Residues at Positions 3-4 of Endomorphin-2
HONDA Takeshi, SHIGEHIRO Daiki, OKADA Kazushi, SHIRASU Naoto, CHUMAN Yoshiro, FUJITA Tsugumi, NOSE Takeru, SAKAGUCHI Kazuyasu, SHIMOHIGASHI Yasuyuki, Peptide science : proceedings of the ... Japanese Peptide Symposium, 2000, 139, 142, 2001年03月01日
英語

Stabilization of Tumor Suppressor Protein p53 through a Phosphorylation Cascade Mediated by DNA Damage
SAKAGUCHI Kazuyasu, SAITO Shin'ichi, HIGASHIMOTO Yuichiro, ROY Siddhartha, ANDERSON Carl W., APPELLA Ettore, Peptide science : proceedings of the ... Japanese Peptide Symposium, 1999, 45, 48, 2000年03月01日
英語

DNA損傷によって誘起されるp53の18位スレオニンのリン酸化によるMDM2結合の阻害　　　　　　　　　　　　　　　
坂口 和靖, 斎藤 伸一, 東元 祐一郎, Roy Siddhartha, Anderson Carl W, Appella Ettore, 生化学, 71, 8, 759, 759, 1999年08月
(公社)日本生化学会, 日本語

Phosphorylation Influences The Association of p53 Tetramers
SAKAGUCHI Kazuyasu, SAKAMOTO Hiroshi, XIE Dong, ERICKSON John W., LEWIS Marc S., ANDERSON Carl W., APPELLA Ettore, Peptide chemistry : proceedings of the ... Symposium on Peptide Chemistry, 1996, 165, 168, 1996年10月01日
英語

Chemical Synthesis of Phosphorylated Peptides of The Carboxy-Terminal Domain of Human p53 by A Segment Condensation Method
SAKAMOTO Hiroshi, KODAMA Hiroaki, HIGASHIMOTO Yuichiro, KONDO Michio, LEWIS Marc S., ANDERSON Carl W., APPELLA Ettore, SAKAGUCHI Kazuyasu, Peptide chemistry : proceedings of the ... Symposium on Peptide Chemistry, 1996, 85, 88, 1996年10月01日
英語

SOLUTION STRUCTURE OF THE HUMAN P53 OLIGOMERIZATION DOMAIN BY THE USE OF SYNTHETIC PEPTIDES AND MULTIDIMENSIONAL NMR
K SAKAGUCHI, H SAKAMOTO, MS LEWIS, H KODAMA, JG OMICHINSKI, AM GRONENBORN, GM CLORE, E APPELLA, PEPTIDE CHEMISTRY 1994, 105, 108, 1995年
英語

Interhelical angles in the solution structure of the oligomerization domain of p53: Correction
Clore,G. M, Omichinski,J. G, Sakaguchi,K, Zambrano,N, Sakamoto,H, Appella,E, Gronenborn,A. M, Science, 267, 1515, 1516, 1995年

Specific sequences from the carboxy terminus of human p53 gene product form anti-parallel teramers in solution
Sakamoto,H, Lewis,M. S, Kodama,H, Appella,E, Sakaguchi,K, Proceedings of the National Academy of Sciences of the United States of America, 91, 8974, 8978, 1994年

STRUCTURE-ACTIVITY-RELATIONSHIPS OF A TETHERED PEPTIDE LIGAND OF THE HUMAN THROMBIN RECEPTOR
K SAKAGUCHI, H KODAMA, Y OGINO, T COSTA, Y SHIMOHIGASHI, PEPTIDE CHEMISTRY 1992, 371, 372, 1993年
英語

[4,4'-(Z)-DEHYDROPHENYLALANINE]GRAMICIDIN-S WITH STABILIZED BIOACTIVE CONFORMATION AND STRONG ANTIMICROBIAL ACTIVITY
Y SHIMOHIGASHI, H KODAMA, S IMAZU, H HORIMOTO, K SAKAGUCHI, M WAKI, H UCHIDA, M KONDO, T KATO, N IZUMIYA, FEBS LETTERS, 222, 2, 251, 255, 1987年10月
英語

Peptide Science 2011　　　　　　　　　　　　　　　
The Japanese Peptide Society, 2012年

自然科学実験　　　　　　　　　　　　　　　
学術図書出版, 2007年

抗生物質に対する細菌の薬剤耐性獲得における新規機構の解明
科学研究費助成事業
2023年06月30日 - 2026年03月31日
坂口 和靖, 鎌田 瑠泉, 中川 夏美
日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 23K17966

リボソームＲＮＡ潜在性ＯＲＦ由来機能性ポリペプチドによる細菌の生存危機防御機構
科学研究費助成事業
2023年04月01日 - 2026年03月31日
坂口 和靖, 鎌田 瑠泉, 中川 夏美
翻訳装置であるリボソームを構成する主要構成分子であるリボソームRNA（rRNA）は、タンパク質をコードしないノンコーディングRNAとされている。本研究では、「細菌のrRNAが潜在的コード情報を内在し、生存危機などの特異な状況においてポリペプチドr-Peptideとして翻訳され機能する」という申請者の仮説の立証を目指す。このため、各種生存危機条件下におけるnLC-MS/MSによるトップダウン的手法による大腸菌r-Peptideの同定と機能解析、バイオインフォマティクスを用いたボトムアップ的手法によるr-Peptideの合成と機能解析を行う。その中で、医学的に特に優れた機能をもつ可能性のあるr-Peptideの機能発現におけるメカニズム解析を実施する。
初年度は、大腸菌rRNA latent ORFにコードされたr-Peptideデータベースの構築を行った。続いて、大腸菌を生存危機ストレスに曝露した際に、rRNAから翻訳されるr-Peptideの分離・同定のための詳細な条件検討を実施した。その結果、カナマイシン添加時に特定のr-Peptideが複数の酸化状態のフラグメントとして存在することが示された。また、異なる配列を有するr-Peptideについても同定された。並行してインフォマティクス解析より得られた大腸菌rRNA latent ORFにコードされたr-Peptideを合成した。今後大腸菌の増殖、形態への効果、r-Peptideの局在解析、各種抗生物質の活性に対する効果を解析する。
本研究により、『原核生物がrRNAを鋳型としてポリペプチドが翻訳する』というセントラルドグマ基本概念の拡張へと繋がる。さらに、この新規メカニズムに基づいた薬剤開発へと展開されることが期待される。
日本学術振興会, 基盤研究(B), 北海道大学, 23K26791

リボソームＲＮＡ潜在性ＯＲＦ由来機能性ポリペプチドによる細菌の生存危機防御機構
科学研究費助成事業
2023年04月01日 - 2026年03月31日
坂口 和靖, 鎌田 瑠泉, 中川 夏美
日本学術振興会, 基盤研究(B), 北海道大学, 23H02098

癌抑制タンパク質p53の一過的機能停止制御を介した新規ゲノム編集法の開発
科学研究費助成事業
2020年04月01日 - 2023年03月31日
坂口 和靖, 鎌田 瑠泉, 中川 夏美
ゲノム編集は、次世代の遺伝子治療法として大きな注目を集めている。しかしながら、CRISPR/Cas9においても、癌抑制タンパク質p53が変異・欠損している細胞に対して優先的にゲノム編集が起こり、治療後に細胞癌化の副作用の恐れがある。このため、有効な治療法が遺伝子治療のみである遺伝性疾患等の治療のために、より安全なゲノム編集法の開発が求められている。安全なゲノム編集のためには、細胞癌化の抑制機構経路の中心であるp53機能を『一過的』に停止制御することが必須である。本研究では、癌抑制タンパク質p53機能の時間的制御による新規ゲノム編集法の開発研究を実施する。すなわち、p53の機能発現に必須な四量体形成を介して、ゲノム編集するときのみp53活性を停止させ、効率的なゲノム編集を達成し、編集後にp53活性を回復させることにより細胞癌化を抑止可能な安全なゲノム編集法の開発を目指す。
初年度は、コイルドコイルペプチドによる機能性ペプチドの多量体化がその生理活性に及ぼす効果を制御することを見出した。今年度は、機能性ペプチドとして我々が発見した抗菌活性ペプチドr-Pep1を用い、その多量体化により大腸菌および哺乳細胞の増殖に対する効果を顕著に変化させることを見出した。さらに、一過的にp53の機能を阻害した際のゲノム編集効率を解析するため、ID-p53Tetペプチドの機能解析のためのp53活性およびゲノム編集効率を同時にモニターするレポーター系を確立した。
本研究における、多量体化を基盤とした新規機能性ペプチドの創成および癌抑制タンパク質p53のヘテロオリゴマー化を介した一過的機能阻害ペプチドID-p53Tetの開発により、安全かつ効率的なゲノム編集法の開発が強く期待される。
日本学術振興会, 基盤研究(B), 北海道大学, 20H02873

細菌リボソームRNAにコードされ生存危機ストレスにより翻訳される防御ポリペプチド
科学研究費助成事業
2019年06月28日 - 2022年03月31日
坂口 和靖, 鎌田 瑠泉
近年、抗生剤の重要性が医学的・社会的にますます大きくなっている。本研究では、ノンコーディングRNAとされている原核生物リボソームRNA（rRNA）が遺伝子情報を内在的にコードし、生存危機回避などの特殊な状況においてポリペプチドに翻訳されるという『r-Peptide』仮説の解明を目指し、rRNA配列由来のポリペプチド（r-peptide）の同定と機能について研究を実施した。バイオインフォマティクス解析より得られたr-peptideが特異的な抗-抗菌活性を有し、その作用機序モデルを示した。さらに、質量分析解析により、抗生物質存在下において酸化型r-peptideの発現が示唆された。
日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 19K22240

配位金属制御に基づく新規な基質トラッピング法による癌抑制ホスファターゼ標的の探索　　　　　　　　　　　　　　　
挑戦的萌芽研究
2015年 - 2016年
坂口 和靖
科研費, 研究代表者, 競争的資金

癌抑制タンパク質ｐ５３の多量体化と配向化を基盤とした生物イベント制御と機能解明　　　　　　　　　　　　　　　
基盤研究（B）
2012年 - 2014年
坂口 和靖
科研費, 研究代表者, 競争的資金

ＰＰＭ１Ｄホスファターゼ過剰発現癌細胞における染色体分配制御の破綻と分子基盤解明　　　　　　　　　　　　　　　
基盤研究（C）
2012年 - 2014年
中馬 吉郎
科研費, 競争的資金

iPS細胞の樹立効率化のためのヘテロオリゴマーを介した癌抑制タンパク質p53阻害　　　　　　　　　　　　　　　
挑戦的萌芽研究
2011年 - 2012年
坂口 和靖
科研費, 研究代表者, 競争的資金

腫瘍由来変異と進化に基づく癌抑制タンパク質p53四量体安定性と機能不全閾値の解明　　　　　　　　　　　　　　　
基盤研究（B）
2009年 - 2011年
坂口 和靖
科研費, 研究代表者, 競争的資金

E‐Life構築を目指したD型タンパク質翻訳系の開発　　　　　　　　　　　　　　　
挑戦的萌芽研究
2009年 - 2010年
坂口 和靖
科研費, 研究代表者, 競争的資金

がん抑制タンパク質p53四量体形成変異による不活性化機構の解明と機能修復剤の開発研　　　　　　　　　　　　　　　
基盤研究（B）
2006年 - 2008年
坂口 和靖
科研費, 研究代表者, 競争的資金

がん抑制タンパク質p53の四量体形成ドメインのフォールディング　　　　　　　　　　　　　　　
特定領域研究
2004年 - 2005年
坂口 和靖
科研費, 研究代表者, 競争的資金

酸化ストレス解析のための特異的モノクローナル抗体作製法の開発　　　　　　　　　　　　　　　
萌芽研究
2003年 - 2004年
坂口 和靖
科研費, 研究代表者, 競争的資金

がん遺伝子治療用の非ヘテロ四量体形成性がん抑制タンパク質p53の設計と開発　　　　　　　　　　　　　　　
基盤研究（B）
2002年 - 2004年
坂口 和靖
科研費, 研究代表者, 競争的資金

Structure and Post-translational Modification of Tumor suppressor Protein p53 and its related proteins　　　　　　　　　　　　　　　
2003年
競争的資金

リン酸化モチーフ特異的抗体を用いたタンパク質リン酸化解析法の開発　　　　　　　　　　　　　　　
基盤研究（C）
2000年 - 2001年
科研費, 競争的資金

p53誘導性ホスファターゼWip1の局在化とSH3蛋白質を介した細胞周期の制御　　　　　　　　　　　　　　　
特定領域研究（C）
2001年
科研費, 競争的資金

p53誘導性ホスファターゼWip1の局在化調節を介した細胞周期の制御機構の解明　　　　　　　　　　　　　　　
特定領域研究（C）
2000年
科研費, 競争的資金

Cell cycle control by protein phosphatase　　　　　　　　　　　　　　　
1999年
競争的資金