Interspecific variation in root morphological traits under salt stress among Larix gmelinii, L. kaempferi, and their hybrid
Tetsuto Sugai, Naoki Makita, Hikari Yahara, Toshihiro Watanabe
Acta Physiologiae Plantarum, 48, 1, Springer Science and Business Media LLC, 31 Dec. 2025
Scientific journal

Cesium accumulation in nodules is involved in mitigating cesium transfer to shoot.
Kazuki Murashima, Naoto Nihei, Nao Okuma, Hayato Maruyama, Toshihiro Watanabe, Takuro Shinano
Scientific reports, 15, 1, 44449, 44449, 24 Dec. 2025, [International Magazine]
English, Scientific journal, Radiocesium (137Cs) transfer from soil to crops is largely regulated by soil potassium (K) levels owing to the chemical similarity between K and cesium (Cs). However, the mitigation of Cs translocation in soybean through soil K is lower than in other crops, highlighting the importance of clarifying soybean-specific Cs translocation mechanisms. Although root nodule symbiosis has been proposed to alter nutrient transport systems, its impact on Cs dynamics remains unclear. We hypothesized that Cs translocation mechanisms are altered under root nodule symbiosis. To elucidate these mechanisms, we conducted field experiments using three soybean genotypes with different nodulation abilities and analyzed their elemental distribution patterns. Additionally, hydroponic experiments using inoculated soybeans were conducted to investigate 137Cs distribution. We found that Cs concentrations were consistently higher in nodules than in other organs. Radioisotope imaging also showed predominant 137Cs accumulation in nodules. Covariance analysis revealed that Cs translocation to shoot was lower in genotypes with higher nodule formation under the same soil exchangeable K conditions. Furthermore, increased nodule formation, especially nodule number, was associated with reduced Cs translocation to shoot. These results suggest that nodules contribute to suppressing Cs translocation to shoot and provide new insights into Cs dynamics under root nodule symbiosis.

Enhancing Molybdenum Availability for Nitrogen Fixation with Manure Biochar: Effect on Soil Conditions and Nutrient Uptake
Mahmudul Islam Piash, Moeka Abe, Takanori Itoh, Kazunori Iwabuchi, Toshihiro Watanabe, Hayato Maruyama
Journal of Soil Science and Plant Nutrition, 25, 4, 9757, 9767, Springer Science and Business Media LLC, 28 Oct. 2025
Scientific journal

4-4-3 マルチオミクスによる牛糞堆肥施与条件下のダイズ農業生態系構造の変動解析 （4-4 植物の代謝成分と農作物の品質）
村島 和基, 丸山 隼人, 渡部 敏裕, 草野 都, 杉山 暁史, 小林 奈通子, 濱本 昌一郎, 福島 敦史, 大津 厳生, 大熊 直生, 藤原 風輝, 二瓶 直登, 市橋 泰範, 信濃 卓郎
日本土壌肥料学会講演要旨集, 71, 74, 74, 一般社団法人 日本土壌肥料学会, 08 Sep. 2025
Japanese

Enhanced CO2 removal and improved carbon budget by enhanced rock weathering: a field experiment in Hokkaido, Japan
Yilin Yang, Gen Kosaka, Hiroshi Uchibayashi, Yan Zhu, Kanta Kuramochi, Takuro Shinano, Toshihiro Watanabe, Hayato Maruyama, Shoichiro Hamamoto, Atsushi Nakao, Yo Toma
Nutrient Cycling in Agroecosystems, Springer Science and Business Media LLC, 06 Jun. 2025
Scientific journal, Abstract

Climate change affects food production, increasing the need for CO₂ removal (CDR) strategies. Enhanced rock weathering, which involves the spreading of crushed silicate rock powder on agricultural soil to sequester atmospheric CO₂ via enhanced natural weathering, shows potential to enrich the agricultural soil. In this study, we evaluated the short-term impacts of basalt powder on CO₂ emissions in rhizosphere and non-rhizosphere soils and estimated the field carbon budget in an experimental soybean field at Hokkaido University, Japan. Basalt powder application at 150 Mg ha⁻¹, with incorporation into the soil to a depth of 15 cm, significantly increased the soil pH and reduced the soil volumetric water content. Regardless of treatment, the carbon budget was negative, indicating the overall carbon loss in field. Basalt powder application reduced this carbon loss from 2.69 ± 0.41 to 1.90 ± 0.73 Mg C ha⁻¹, primarily by absorbing CO₂ that would otherwise have been released into the atmosphere through weathering and sequestering it in the soil, though the difference was not significant. ERW-induced CO₂ emission reduction rate was 0.81 ± 0.17 Mg C ha⁻¹, with approximately 86.4% attributed to rhizosphere soil. In the rhizosphere, basalt application significantly reduced CO₂ emissions, suggesting that root exudates may promote basalt weathering and increase stabilization of carbon in the rhizosphere through interactions with the rock powder, thereby contributing to the observed emission reduction. These findings indicate that basalt application can significantly reduce CO₂ emissions from agricultural soils, particularly in the rhizosphere, and could potentially improve the overall farmland carbon balance. However, further research is necessary to explore the long-term effects of ERW on soil carbon dynamics and elucidate the differential responses of soil organic and inorganic carbon pools, especially considering that the applied basalt powder is unlikely to completely weather within a single year, and thus its CO₂ removal effects may persist over multiple growing seasons.

HalALMT1 mediates malate efflux in the cortex of mature cluster rootlets of Hakea laurina, occurring naturally in severely phosphorus-impoverished soil.
Hirotsuna Yamada, Lydia Ratna Bunthara, Akira Tanaka, Takuro Kohama, Hayato Maruyama, Wakana Tanaka, Sho Nishida, Tantriani, Akira Oikawa, Keitaro Tawaraya, Toshihiro Watanabe, Shu Tong Liu, Patrick M Finnegan, Hans Lambers, Takayuki Sasaki, Jun Wasaki
The New phytologist, 246, 6, 2597, 2616, Jun. 2025, [International Magazine]
English, Scientific journal, Hakea laurina, a woody Proteaceae, naturally occurs in severely phosphorus (P)-impoverished habitats in southwest Australia. It develops distinctive cluster roots that exhibit a high capacity for carboxylate exudation and acid phosphatase activity, contributing to its P acquisition. However, the molecular mechanisms underlying these physiological functions remain poorly understood. We explored the cluster-root transcriptome using de novo RNA-Seq and identified Hakea laurina Aluminum-activated Malate Transporter 1 (HalALMT1), encoding an aluminum (Al)-activated malate transporter induced in mature cluster roots. We characterized HalALMT1 through electrophysiological assays and overexpression in Arabidopsis thaliana, and localized HalALMT1 expression, acid phosphatase activity, and suberized boundaries in cluster roots. Differentially expressed genes highlighted multiple increased carboxylate-related processes at cluster-root maturity. HalALMT1 released malate, an activity further enhanced by exposure to Al3+. Notably, HalALMT1 was specifically expressed in mature cortex cells of cluster rootlets, which lack a suberized exodermis. Acid phosphatase activity was pronounced throughout the cluster rootlets, unlike in noncluster roots where it was limited to the epidermis and stele. Substantial malate release and acid phosphatase activity in the cortex cells in cluster rootlets, which lack a suberized exodermis, allowed massive exudation. This study sheds light on an exquisite P-acquisition strategy of Proteaceae, enabling survival under extremely low P availability.

Why can Palhinhaea cernua (lycophyte) grow closer to fumaroles in highly acidic solfatara fields?
Toshihiro Watanabe, Nozomi Imai, Syuntaro Hiradate, Hayato Maruyama, Jun Wasaki
Journal of plant research, 138, 1, 19, 35, Jan. 2025, [Domestic magazines]
English, Scientific journal, Palhinhaea cernua, a lycophyte, and Dicranopteris linearis, a fern, are commonly observed in solfatara fields in Kyushu, Japan, but their distribution trends are different. The aim of this study was to determine why P. cernua is more abundant in areas closer to fumaroles from both a soil and plant perspective. Samples of P. cernua and D. linearis, as well as their respective growing soils, were collected, and the mineral properties, including the concentration of various mineral elements and inorganic anions and δ15N, were determined. P. cernua was better adapted to soil with lower pH, higher soluble aluminum concentrations, and poorer calcium and phosphorus concentrations than D. linearis. A positive correlation was observed between shoot nitrogen concentration and both shoot sulfur concentration and soil water-soluble sulfur concentration in P. cernua, implying the involvement of sulfur in nitrogen acquisition in P. cernua. The results also suggested that D. linearis mainly uses soil NO3-N, while P. cernua uses NH4-N, which is predominant and excessive in the solfatara fields, particularly near the fumaroles. This high preference for NH4-N in P. cernua was confirmed through a cultivation experiment. While D. linearis prefers NO3-N and distributes further from fumaroles, P. cernua may have survived in the solfatara fields by utilizing NH4-N and sulfur, which are abundant near fumaroles where competition from other plant species is minimal.

Evaluation of Functional Components in Paprika Fruits (<i>Capsicum annuum</i> L.) under Different Nitrogen Concentrations
Kazuki Murashima, Ryota Arakawa, Hayato Maruyama, Toshihiro Watanabe, Takuro Shinano
The Horticulture Journal, Japanese Society for Horticultural Science, 2025
Scientific journal

Impact of basalt application on soil chemical properties and elemental uptake by paddy rice through enhanced rock weathering
Hiroshi Uchibayashi, Hayato Maruyama, Toshihiro Watanabe, Shoichiro Hamamoto, Yo Toma, Atsushi Nakao, Kohei Kurokawa, Takuro Shinano
Soil Science and Plant Nutrition, 2025
Scientific journal

Concentrations and inter‐element correlations of seven essential elements in wild plants of Japan
Takafumi Mizuno, Daichi Kondo, Hiroto Kasai, Kosuke Kuwabara, Jun Yamashita, Yoshinori Murai, Antony van der Ent, Atsushi Hashimoto, Toshihiro Watanabe
Ecological Research, Wiley, 10 Oct. 2024, [Peer-reviewed], [Last author]
English, Scientific journal, Abstract

We assessed the effects of soil type on the concentrations of seven elements (calcium, potassium, sulfur, phosphorus, iron, manganese, zinc) in plants using x‐ray fluorescence (XRF) analysis and plant specimens collected from calcareous and ultramafic soil areas, and ‘general soils’ (other types of soil represented by Brown Forest soils and Andosols) of Japan. Compared with the plants from other types of soils, the means of these seven elements were lower in plants originating from ultramafic soils, especially in phosphorus. Plants from calcareous soil areas had high iron and zinc concentrations on average, but this tendency was biased by plants collected on Mt. Fujiwara, which had extremely high average values of these elements. Calcium concentration in plants had a negative correlation with zinc or iron from calcareous or general soils, respectively, and iron showed positive correlations with zinc, manganese, and sulfur in plants from calcareous, ultramafic, and general soils, respectively. These correlations were not found in plants from general soils, suggesting that these relationships reflect the chemical characteristics of soil types. On the contrary, plants from all soil types showed a positive correlation between sulfur and phosphorus, whereas a positive correlation between potassium and phosphorus was found in plants from calcareous and general soils, but not in ultramafic plants, which instead showed a weak, but statistically significant correlation between potassium and sulfur. Statistically, iron and sulfur concentrations were positively correlated in all soil types, but manganese showed a low correlation with the other elements.

Decay Resistance of Citric Acid Cross-Linked Paper
Takuya Nakagawa, Toshihiro Watanabe, Hirokatsu Uchino
Journal of Fiber Science and Technology, 80, 9, 197, 203, Society of Fiber Science and Technology Japan, 10 Sep. 2024, [Peer-reviewed]
Scientific journal

8-2-2 畑地への玄武岩施与が土壌化学性およびダイズの生育に及ぼす影響と風化促進の可能性（8-2 地球環境 2024年度福岡大会）
内林 大志, 丸山 隼人, 渡部 敏裕, 当真 要, 濱本 昌一郎, 楊 椅麟, 朱 顔, 小坂 弦, 中尾 淳, 黒川 耕平, 信濃 卓郎
日本土壌肥料学会講演要旨集, 70, 152, 152, 一般社団法人 日本土壌肥料学会, 26 Aug. 2024
Japanese

Effects of soil and phylogeny on sulfur and phosphorus concentrations in wild plants on volcanic and non-volcanic soils in Japan
Takafumi Mizuno, Daichi Kondo, Hiroto Kasai, Jun Yamashita, Takuro Ito, Yoshinori Murai, Antony van der Ent, Atsushi Hashimoto, Toshihiro Watanabe
Soil Science and Plant Nutrition, 1, 11, Informa UK Limited, 22 Jul. 2024, [Peer-reviewed], [Last author]
Scientific journal

Alleviating effect of calcium sulfate on aluminum stress through enhanced malate release with increased gene expression of malate transporter and sulfate transporter
Koffi Pacome Kouame, Raj Kishan Agrahari, Tasuku Miyachi, Yasufumi Kobayashi, Toshihiro Watanabe, Toshiki Nakamura, Akiko Maruyama-Nakashita, Masafumi Shimizu, Hiroyuki Koyama, Yuriko Kobayashi
Soil Science and Plant Nutrition, 2024
Scientific journal

Ecophysiological difference in co-existing beech and oak saplings grown in different soil types under a free-air ozone exposure system
Satoshi Kitaoka, Cong Shi, Toshihiro Watanabe, Takayoshi Koike
Journal of Forest Research, 29, 1, 30, 37, Informa UK Limited, 20 Dec. 2023, [Peer-reviewed]
Scientific journal

Leaf defense traits of birch, beech, and oak saplings grown under two types of soil in a free-air ozone exposure system
Cong Shi, Noburu Masui, Takayoshi Koike, Chisato Terada, Masahiro Nakamura, Toshihiro Watanabe
Journal of Forest Research, online first, 1, 11, Informa UK Limited, 16 Nov. 2023, [Peer-reviewed]
Scientific journal

Zero-valent iron reduces cadmium and arsenic accumulation in spinach ( Spinacia oleracea L.)
Takashi Nakamura, Mitsuru Osaki, Toshihiro Watanabe
Journal of Plant Nutrition, 47, 4, 646, 655, Informa UK Limited, 09 Nov. 2023, [Peer-reviewed]
Scientific journal

Effects of cattle manure compost application on crop growth and soil-to-crop transfer of cesium in a physically radionuclide-decontaminated field
Masataka Suzuki, Katashi Kubo, Mayumi Hachinohe, Takashi Sato, Hirofumi Tsukada, Noriko Yamaguchi, Toshihiro Watanabe, Hayato Maruyama, Takuro Shinano
Science of The Total Environment, 167939, Nov. 2023, [Peer-reviewed]

Essential Role of Symbiotic Microorganisms Supporting Forests in East Asia under Changing Environment
Wang YN, Qu LY, Tamai Y, Watanabe T, Koike T
IntechOpen, https://www.intechopen.com/chapters/86879, 2, 26, Jun. 2023, [Peer-reviewed]
In book

Bacterial Communities and Soil Properties Influencing Dung Decomposition and Gas Emissions Among Japanese Dairy Farms
Chidozie J. Oraegbunam, Akari Kimura, Terumasa Yamamoto, Yvonne M. Madegwa, Sunday E. Obalum, Chikae Tatsumi, Toshihiro Watanabe, Yoshitaka Uchida
Journal of Soil Science and Plant Nutrition, 23, 3, 3343, 3348, Springer Science and Business Media LLC, 27 Apr. 2023, [Peer-reviewed]
Scientific journal

Landscape gradient of autumn photosynthetic decline in Abies sachalinensis seedlings
Tetsuto Sugai, Wataru Ishizuka, Toshihiro Watanabe
Journal of Forestry Research, 34, 1, 187, 195, Springer Science and Business Media LLC, Feb. 2023, [Peer-reviewed]
Scientific journal, Abstract

Understanding what environmental factors are genetically linked to a phenological event is critical for predicting responses to climate change. Photosynthetic phenology often varies among a species of evergreen conifers due to local adaptation. However, few empirical studies have revealed relevant relationships between climatic factors in provenance environments and photosynthetic phenology. This study evaluated the effects of environmental conditions of the growing site and seed source provenance on the seasonal changes in maximal photochemical quantum yield of photosystem II (F_v/F_m) in a common garden experiment with 2-year-old seedlings of Sakhalin fir (Abies sachalinensis), a representative species with local adaptation, from four seed source provenances. A logistic model was constructed to explain the seasonal variation of F_v/F_m from July to October and the relationships between the estimated model parameters and representative factors featuring provenance environments were evaluated. The landscape gradient of the detected model parameters responsible for the provenance environments was visualized in a map of the distribution area. The lowest temperature was the most plausible factor in the growing environment to explain the seasonal changes of F_v/F_m. Among the representative meteorological factors of provenance environments, the lowest temperatures in July showed significant relationships with two model parameters, explaining the lower limit of F_v/F_m and the higher sensitivity of autumn F_v/F_m decline. The estimated spatial maps of model parameters consistently showed that the higher the lowest temperature in July in the provenance environment, the lower the F_v/F_m in October and the greater the decrease in the autumn F_v/F_m decline. Therefore, the lowest summer temperature could be associated with the local adaptation of autumn photosynthetic phenology in A. sachalinensis.

Different nitrogen acquirement and utilization strategies might determine the ecological competition between ferns and angiosperms
Chengming Zhang, Chaoqun Zhang, Takayuki Azuma, Hayato Maruyama, Takuro Shinano, Toshihiro Watanabe
Annals of Botany, Oxford University Press (OUP), 20 Jan. 2023
Scientific journal, Abstract

Background and Aims

The abundance or decline of fern populations in response to environmental change has been found to be largely dependent on specific physiological properties that distinguish ferns from angiosperms. Many studies have focused on water use efficiency and stomatal behaviours, but the effects of nutrition acquirement and utilization strategies on niche competition between ferns and flowering plants are rarely reported.

Methods

We collected 34 ferns and 42 angiosperms from the Botanic Garden of Hokkaido University for nitrogen (N), sulphur (S), NO3− and SO42− analysis. We then used a hydroponic system to compare the different N and S utilization strategies between ferns and angiosperms under N deficiency conditions.

Key Results

Ferns had a significantly higher NO3−-N concentration and NO3−-N/N ratio than angiosperms, although the total N concentration in ferns was remarkably lower than that in the angiosperms. Meanwhile, a positive correlation between N and S was found, indicating that nutrient concentration is involved in assimilation. Pteris cretica, a fern species subjected to further study, maintained a slow growth rate and lower N requirement in response to low N stress, while both the biomass and N concentration in wheat (Triticum aestivum) responded quickly to N deficiency conditions.

Conclusions

The different nutritional strategies employed by ferns and angiosperms depended mainly on the effects of phylogenetic and evolutionary diversity. Ferns tend to adopt an opportunistic strategy of limiting growth rate to reduce N demand and store more pooled nitrate, whereas angiosperms probably utilize N nutrition to ensure as much development as possible under low N stress. Identifying the effects of mineral nutrition on the evolutionary results of ecological competition between plant species remains a challenge.

Potassium application reduced cesium uptake and altered strontium translocation in soybean plants
Syaiudin, M, Suzuki, M, Maruyama, H, Kubo, K, Watanabe, T, Shinano, T
Soil Science and Plant Nutrition, Jan. 2023, [Peer-reviewed]
Scientific journal

Identification of a Saltol-Independent Salinity Tolerance Polymorphism in Rice Mekong Delta Landraces and Characterization of a Promising Line, Doc Phung.
Tam Thanh Nguyen, Maria Stefanie Dwiyanti, Shuntaro Sakaguchi, Yohei Koide, Dung Viet Le, Toshihiro Watanabe, Yuji Kishima
Rice (New York, N.Y.), 15, 1, 65, 65, 18 Dec. 2022, [International Magazine]
English, Scientific journal, The Mekong Delta River in Vietnam is facing salinity intrusion caused by climate change and sea-level rise that is severely affecting rice cultivation. Here, we evaluated salinity responses of 97 rice accessions (79 landraces and 18 improved accessions) from the Mekong Delta population by adding 100 mM NaCl to the nutrient solution for up to 20 days. We observed a wide distribution in salinity tolerance/sensitivity, with two major peaks across the 97 accessions when using the standard evaluation system (SES) developed by the International Rice Research Institute. SES scores revealed strong negative correlations (ranging from - 0.68 to - 0.83) with other phenotypic indices, such as shoot elongation length, root elongation length, shoot dry weight, and root dry weight. Mineral concentrations of Na+ in roots, stems, and leaves and Ca2+ in roots and stems were positively correlated with SES scores, suggesting that tolerant accessions lower their cation exchange capacity in the root cell wall. The salinity tolerance of Mekong Delta accessions was independent from the previously described salinity tolerance-related locus Saltol, which encodes an HKT1-type transporter in the salinity-tolerant cultivars Nona Bokra and Pokkali. Indeed, genome-wide association studies using SES scores and shoot dry weight ratios of the 79 accessions as traits identified a single common peak located on chromosome 1. This SNP did not form a linkage group with other nearby SNPs and mapped to the 3' untranslated region of gene LOC_Os01g32830, over 6.5 Mb away from the Saltol locus. LOC_Os01g32830 encodes chloroplast glycolate/glycerate translocator 1 (OsPLGG1), which is responsible for photorespiration and growth. SES and shoot dry weight ratios differed significantly between the two possible haplotypes at the causal SNP. Through these analyses, we characterize Doc Phung, one of the most salinity-tolerant varieties in the Mekong Delta population and a promising new genetic resource.

Effect of Light Quality on Metabolomic, Ionomic, and Transcriptomic Profiles in Tomato Fruit
Lingran Xiao, Tomoki Shibuya, Toshihiro Watanabe, Kazuhisa Kato, Yoshinori Kanayama
International Journal of Molecular Sciences, 23, 21, 13288, 13288, MDPI AG, 31 Oct. 2022
Scientific journal, Light quality affects plant growth and the functional component accumulation of fruits. However, there is little knowledge of the effects of light quality based on multiomics profiles. This study combined transcriptomic, ionomic, and metabolomic analyses to elucidate the effects of light quality on metabolism and gene expression in tomato fruit. Micro-Tom plants were grown under blue or red light-emitting diode light for 16 h daily after anthesis. White fluorescent light was used as a reference. The metabolite and element concentrations and the expression of genes markedly changed in response to blue and red light. Based on the metabolomic analysis, amino acid metabolism and secondary metabolite biosynthesis were active in blue light treatment. According to transcriptomic analysis, differentially expressed genes in blue and red light treatments were enriched in the pathways of secondary metabolite biosynthesis, carbon fixation, and glycine, serine, and threonine metabolism, supporting the results of the metabolomic analysis. Ionomic analysis indicated that the element levels in fruits were more susceptible to changes in light quality than in leaves. The concentration of some ions containing Fe in fruits increased under red light compared to under blue light. The altered expression level of genes encoding metal ion-binding proteins, metal tolerance proteins, and metal transporters in response to blue and red light in the transcriptomic analysis contributes to changes in the ionomic profiles of tomato fruit.

The ratio of plant 137Cs to exchangeable 137Cs in soil is a crucial factor in explaining the variation in 137Cs transferability from soil to plant.
Masataka Suzuki, Tetsuya Eguchi, Kazuki Azuma, Atsushi Nakao, Katashi Kubo, Shigeto Fujimura, Muhamad Syaifudin, Hayato Maruyama, Toshihiro Watanabe, Takuro Shinano
The Science of the total environment, 857, Pt 1, 159208, 159208, 05 Oct. 2022, [International Magazine]
English, Scientific journal, To mitigate radioactive cesium from soil to plant, increasing and maintaining the exchangeable potassium (ExK) level during growth is widely accepted after Tokyo Electric Company's Fukushima Dai-ichi Nuclear Plant accident in Japan. This is because the antagonistic relationship between soil solution K and 134Cs + 137Cs (RCs) concentrations changes the transfer factor (TF: designated as the ratio of radioactivity of plant organ to soil) of RCs. As the relationship between ExK and TF depends on the soil types, crop species, and other environmental factors, the required amount of ExK should be set to a safe side. Eleven years after the accident, as the activity of 134Cs was almost negligible, 137Cs became the main RCs in most of the agricultural fields in Fukushima Prefecture. We propose a new indicator, the concentration ratio of plant 137Cs to soil exchangeable 137Cs (Ex137Cs), instead of TF, which showed a better correlation with ExK even among soils with different properties (or mineralogy).

Vigor and Health of Urban Green Resources under Elevated O₃ in Far East Asia
Laiye Qu, Yannan Wang, Cong Shi, Xiaoke Wang, Noboru Masui, Thomas Rötzer, Toshihiro Watanabe, Takayoshi Koike
IntechOpen (Environmental Sciences) series, IntechOpen, 29 Sep. 2022, [Peer-reviewed]
In book, Conservation of urban greens is an essential action for city residents, however, declining symptoms and/or traces in the annual ring of trees grown are found in parks and forest stands in a city as well as its suburb with a high level of ozone (O3). Urban greens, including roof-green, provide comfortable conditions for the people and a moderate environment in a city. They are exposed to severe environments; heat, drought, air-pollutions, etc. even with intensive management of the people. How can we proceed with the conservation and wise use of urban greens? We should know the ecophysiological responses of urban trees to such a global environment as well as a local one. Defensive capacities of urban greens should be analyzed in terms of damages caused by biotic and abiotic stresses, and it is important to understand their interactions from the viewpoint of plant-insect/disease. There is a concern that some green areas are suffering from an outbreak of insects and diseases, reducing the vigor and health of urban greens. We discuss these based on specific examples, such as man-made forests, in cities in far east Asia for considering our approach to how to keep urban green resources.

Analysis of varietal differences in grain productivity and seed mineral content of oats
Ishii Rei, Maruyama Hayato, Watanabe Toshihiro, Shinano Takuro, Kubo Katashi
Abstracts of Meeting of the CSSJ, 254, 12, 12, CROP SCIENCE SOCIETY OF JAPAN, 18 Sep. 2022
Japanese

P4-3-9 アルミニウム集積植物メラストーマにおけるFRD3タイプMATE のアルミニウム輸送への関与（4-3 植物の有害元素 2022年度東京大会）
坂口 文香, 丸山 隼人, 佐々木 孝行, 西田 翔, 和崎 淳, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 68, 59, 59, 一般社団法人 日本土壌肥料学会, 05 Sep. 2022
Japanese

4-3-7 Al 耐性遺伝子PGIP1 の発現量ゲノムワイド関連解析によるPGIP1 発現を制御するシグナル伝達経路と遺伝子の同定（4-3 植物の有害元素 2022年度東京大会）
榎本 拓央, 伊藤 弘樹, 中野 友貴, 柳瀬 笑子, 渡部 敏裕, 井内 聖, 小林 正智, 山本 義治, 小山 博之, 小林 佑理子
日本土壌肥料学会講演要旨集, 68, 55, 55, 一般社団法人 日本土壌肥料学会, 05 Sep. 2022
Japanese

4-4-4 パプリカ果実における部位別の元素動態と尻腐れ果発症との関係（4-4 植物の代謝成分と農作物の品質 2022年度東京大会）
荒川 竜太, 鳥山 星呂, アクリッシュ 穂波, 渡部 敏裕, 信濃 卓郎, 丸山 隼人
日本土壌肥料学会講演要旨集, 68, 62, 62, 一般社団法人 日本土壌肥料学会, 05 Sep. 2022
Japanese

Responses of ectomycorrhizal diversity of larch and its hybrid seedlings and saplings to elevated CO2, O3, and high nitrogen loadin
QU, Laiye, WANG, Xiaona, MAO, Qiaozhi, AGATHOKLEOUS, Evgenios, CHOI, DongSu, TAMAI, Yutaka, WATANABE, Toshihiro, KOIKE
Eurasian Journal of Forest Research, 22, 23, 27, May 2022, [Peer-reviewed], [Invited]
Research institution

Growth and nitrogen use characteristics of black locust, an invasive alien species, grown under different light and CO₂ conditions
Satoshi Kitaoka, Saki Fujita, Yoko Watanabe, DongSu Choi, Toshihiro Watanabe, Takuro Shinano, Fuyuki Satoh, Takayoshi Koike
Eurasian Journal of Forests Research, 22, 28, 32, Apr. 2022, [Peer-reviewed], [Invited]
English, Research institution

Differences in carbon and nitrogen retention and bacterial diversity in sandy soil in response to application methods of charred organic materials
Chidozie J. Oraegbunam, Sunday E. Obalum, Toshihiro Watanabe, Yvonne M. Madegwa, Yoshitaka Uchida
APPLIED SOIL ECOLOGY, 170, Feb. 2022
English, Scientific journal

Effects of soil compaction on the seedlings growth and ectomycorrhizal fungal community in hybrid larch
SUGAI, Tetsuto, YOKOYAMA, Satoko, TAMAI, Yutaka, MORI, Hirotaka, MARCHI, Enrico, WATANABE, Toshihiro, SATOH, Fuyuki, KOIKE, Takayoshi
Eurasian journal of forest research, 22, 78, 80, 2022

Relationship between soil phosphorus dynamics and low-phosphorus responses at specific root locations of white lupine
Ayane Kan, Hayato Maruyama, Nao Aoyama, Jun Wasaki, Yoshiko Tateishi, Toshihiro Watanabe, Takuro Shinano
Soil Science and Plant Nutrition, 2022, [Peer-reviewed]
Scientific journal

シロバナルーピンの放射性セシウム吸収と体内輸送の品種間差
丸山 隼人, 菅 あやね, 浅枝 諭史, 久保 堅司, 古川 純, 渡部 敏裕, 信濃 卓郎
アイソトープ・放射線研究発表会, 2, 126, 公益社団法人 日本アイソトープ協会, 2022
Japanese

Ionomic Profiling of Rice Genotypes and Identification of Varieties with Elemental Covariation Effects
Zhang Chengming, Nobuhiro Tanaka, Maria Stefanie Dwiyanti, Matthew Shenton, Hayato Maruyama, Takuro Shinano, Chu Qingnan, Xie Jun, Toshihiro Watanabe
Rice Science, 29, 1, 76, 88, Elsevier BV, Jan. 2022
Scientific journal

Expression GWAS of PGIP1 Identifies STOP1-Dependent and STOP1-Independent Regulation of PGIP1 in Aluminum Stress Signaling in Arabidopsis
Raj Kishan Agrahari, Takuo Enomoto, Hiroki Ito, Yuki Nakano, Emiko Yanase, Toshihiro Watanabe, Ayan Sadhukhan, Satoshi Iuchi, Masatomo Kobayashi, Sanjib Kumar Panda, Yoshiharu Y. Yamamoto, Hiroyuki Koyama, Yuriko Kobayashi
Frontiers in Plant Science, 12, Frontiers Media SA, 17 Dec. 2021
Scientific journal, To elucidate the unknown regulatory mechanisms involved in aluminum (Al)-induced expression of POLYGALACTURONASE-INHIBITING PROTEIN 1 (PGIP1), which is one of the downstream genes of SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) regulating Al-tolerance genes, we conducted a genome-wide association analysis of gene expression levels (eGWAS) of PGIP1 in the shoots under Al stress using 83 Arabidopsis thaliana accessions. The eGWAS, conducted through a mixed linear model, revealed 17 suggestive SNPs across the genome having the association with the expression level variation in PGIP1. The GWAS-detected SNPs were directly located inside transcription factors and other genes involved in stress signaling, which were expressed in response to Al. These candidate genes carried different expression level and amino acid polymorphisms. Among them, three genes encoding NAC domain-containing protein 27 (NAC027), TRX superfamily protein, and R-R-type MYB protein were associated with the suppression of PGIP1 expression in their mutants, and accordingly, the system affected Al tolerance. We also found the involvement of Al-induced endogenous nitric oxide (NO) signaling, which induces NAC027 and R-R-type MYB genes to regulate PGIP1 expression. In this study, we provide genetic evidence that STOP1-independent NO signaling pathway and STOP1-dependent regulation in phosphoinositide (PI) signaling pathway are involved in the regulation of PGIP1 expression under Al stress.

Nitrogen deficiency-induced molybdenum accumulation in wheat
Toshihiro Watanabe, Ryoskuke Okada, Soyoka Tokunaga, Hayato Maruyama, Masaru Urayama, Takuro Shinano
JOURNAL OF PLANT NUTRITION, 45, 9, 1413, 1424, Dec. 2021
English, Scientific journal

Possible solubilization of various mineral elements in the rhizosphere of Lupinus albus L.
Atsuhide Takao, Jun Wasaki, Hisae Fujimoto, Hayato Maruyama, Takuro Shinano, Toshihiro Watanabe
Soil Science and Plant Nutrition, 1, 8, Informa UK Limited, 07 Oct. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal, 33048185

4-1-4 シロバナルーピン根域における土壌中リン動態に与える影響の局所的評価（4-1 植物の多量栄養素 2021年度北海道大会）
菅 あやね, 丸山 隼人, 和崎 淳, 渡部 敏裕, 信濃 卓郎
日本土壌肥料学会講演要旨集, 67, 45, 45, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

8-1-11 異なる圃場におけるダイズへの放射性セシウムの移行比較（8-1 環境保全 2021年度北海道大会）
鈴木 政崇, 藤本 久恵, 浅枝 諭史, 丸山 隼人, 久保 堅司, 藤村 恵人, 渡部 敏裕, 信濃 卓郎
日本土壌肥料学会講演要旨集, 67, 126, 126, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

8-1-10 シロバナルーピンの放射性セシウム吸収特性と根圏土壌中元素動態の関係（8-1 環境保全 2021年度北海道大会）
浅枝 諭史, 丸山 隼人, 藤本 久恵, 久保 堅司, 藤村 恵人, 渡部 敏裕, 信濃 卓郎
日本土壌肥料学会講演要旨集, 67, 126, 126, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

P4-1-19 トマト尻腐れ発症率の品種間差と果実への無機元素蓄積の関係（4-1 植物の多量栄養素 2021年度北海道大会）
長谷川 公紀, 渡邊 涼太郎, 丸山 隼人, 金山 喜則, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 67, 58, 58, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

4-3-8 火山性強酸性土壌に生育する植物の葉身元素組成の特徴（4-3 植物の有害元素 2021年度北海道大会）
和崎 淳, 山本 晃弘, 丸山 隼人, 中坪 孝之, 渡部 敏裕
日本土壌肥料学会講演要旨集, 67, 71, 71, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

4-1-1 ミナトカモジグサの生育および養分状態に応じたリン獲得の分子制御機構（4-1 植物の多量栄養素 2021年度北海道大会）
永山 航平, 菅井 徹人, 佐藤 匠, 熊石 紀恵, 市橋 泰範, 渡部 敏裕, 信濃 卓郎, 丸山 隼人
日本土壌肥料学会講演要旨集, 67, 44, 44, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

4-1-3 コムギコアコレクションを用いた可塑的土壌リン獲得形質の品種間差（4-1 植物の多量栄養素 2021年度北海道大会）
青山 奈央, 渡部 敏裕, 信濃 卓郎, 丸山 隼人
日本土壌肥料学会講演要旨集, 67, 44, 44, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

4-4-1 根圏の有機物・ガスに関する植物栄養学研究の一視点—根の細胞壁・細胞膜での各種の化学的・物理的な力と下流遺伝子群への注目（4-4 植物の代謝成分と農作物の品質 2021年度北海道大会）
我妻 忠雄, 俵谷 圭太郎, 程 為国, 渡部 敏裕, 丸山 隼人, 和崎 淳
日本土壌肥料学会講演要旨集, 67, 76, 76, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

4-1-5 異なる土壌における白花ルーピンの根分泌物分布特性の可視化（4-1 植物の多量栄養素 2021年度北海道大会）
尹 永根, 鈴井 伸郎, 三好 悠太, 海野 佑介, 丸山 隼人, 和崎 淳, 菅 あやね, 圓尾 明子, 渡部 敏裕, 橋本 洋平, 内田 翔子, 信濃 卓郎, 河地 有木
日本土壌肥料学会講演要旨集, 67, 45, 45, 一般社団法人 日本土壌肥料学会, 03 Sep. 2021
Japanese

Ionomic differences between tomato introgression line IL8–3 and its parent cultivar M82 with different trends to the incidence of blossom-end rot
Toshihiro Watanabe, Ryota Tomizaki, Ryotaro Watanabe, Hayato Maruyama, Takuro Shinano, Masaru Urayama, Yoshinori Kanayama
Scientia Horticulturae, 287, 110266, 110266, Sep. 2021, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, 12149803

Differences in mineral accumulation and gene expression profiles between two metal hyperaccumulators, Noccaea japonica and Noccaea caerulescens ecotype Ganges, under excess nickel condition.
Takuo Enomoto, Junko Yoshida, Takafumi Mizuno, Toshihiro Watanabe, Sho Nishida
Plant signaling & behavior, 16, 10, 1945212, 1945212, 06 Jul. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Here we compare mineral accumulation and global gene expression patterns between two metal hyperaccumulator plants - Noccaea japonica, originating from Ni-rich serpentine soils, and Noccaea caerulescens (ecotype Ganges), originating from Zn/Pb-mine soils - under excess Ni conditions. Significant differences in the accumulation of K, P, Mg, B, and Mo were explained by the expression levels of specific transporters for each mineral. We previously showed that total Ni accumulation in the whole plant is higher in N. caerulescens than in N. japonica. Here we found a similar tendency for Fe under excess Ni; however, the expression of iron-regulated transporter 1 (IRT1), which encodes the primary Fe uptake transporter and causes excess Ni uptake in Arabidopsis thaliana, was higher in N. japonica. NjIRT1 has a point mutation at Asp100, which is essential for Fe transport, and so might lack its Fe and possibly Ni transport function. Noccaea japonica might have lost its IRT1 function, which would prevent excess Ni uptake via IRT1 in Ni-rich soils, and come to rely on other transporters.

Zerovalent Iron Modulates the Influence of Arsenic-Contaminated Soil on Growth, Yield and Grain Quality of Rice
Sanjida Akter, Golum Kibria Muhammad Mustafizur Rahman, Mirza Hasanuzzaman, Zakaria Alam, Toshihiro Watanabe, Tofazzal Islam
Stresses, Apr. 2021
Scientific journal

Ionomic variation in leaves of 819 plant species growing in the botanical garden of Hokkaido University, Japan.
Toshihiro Watanabe, Takayuki Azuma
Journal of plant research, 134, 2, 291, 304, Mar. 2021, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
English, Scientific journal, Ionomics is the measurement of total metal, metalloid, and nonmetal accumulation in living organisms. Plant ionomics has been applied to various types of research in the last decade. It has been reported that the ionome of a plant is strongly affected by its evolution and by environmental factors. In this study, we analyzed the concentration of 23 elements in leaves of 819 plant species (175 families) growing in the Botanic Garden of Hokkaido University, Japan. Relative variation estimated by the coefficient of variation in foliar concentrations of essential elements among various plant species tended to be low, whereas nickel concentration showed exceptionally large relative variation. By contrast, the relative variation in nonessential elements was high, particularly in sodium, aluminum, and arsenic. The higher relative variations in these element concentrations can be explained by the occurrence of plants that are hyperaccumulators for these elements. Differences in life forms such as herbaceous/woody species, deciduous/evergreen woody species and annual/perennial herbaceous species affected the concentration of several elements in the leaves. These differences were considered to be due to the combined factors including differences in lifespan, growth rate, and cell wall thickness of the leaves. Results of principal component analyses (based on concentration data of essential and nonessential elements in leaf samples) indicated phylogenetic influences on plant ionomes at the family level in Polypodiales, Pinales, Poales, and Ericales. Furthermore, when analyzing correlations among concentrations of all elements in each order and comparing among different orders, the results also suggested that Polypodiales, Pinales, and Poales each had a specific ion homeostasis network.

Correction to: Ionomic variation in leaves of 819 plant species growing in the botanical garden of Hokkaido University, Japan.
Toshihiro Watanabe, Takayuki Azuma
Journal of plant research, 134, 2, 305, 305, Mar. 2021, [Domestic magazines]
English

P4-4-3 温度処理にともなう元素吸収・分配がパプリカの尻腐れ果発症に与える影響（4-4 植物の代謝成分と農作物の品質 2021年度北海道大会）
鳥山 星呂, 荒川 竜太, 村島 和基, 丸山 隼人, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 67, 78, 78, 一般社団法人 日本土壌肥料学会, 2021
Japanese

4-4-2 異なる窒素条件下におけるパプリカ果実の元素組成と尻腐れ果発症との関係（4-4 植物の代謝成分と農作物の品質 2021年度北海道大会）
荒川 竜太, 村島 和基, 渡部 敏裕, 信濃 卓郎, 丸山 隼人
日本土壌肥料学会講演要旨集, 67, 76, 76, 一般社団法人 日本土壌肥料学会, 2021
Japanese

4-4-3 異なる窒素条件下におけるパプリカ果実の機能性成分解析（4-4 植物の代謝成分と農作物の品質 2021年度北海道大会）
村島 和基, 荒川 竜太, 丸山 隼人, 渡部 敏裕, 信濃 卓郎
日本土壌肥料学会講演要旨集, 67, 76, 76, 一般社団法人 日本土壌肥料学会, 2021
Japanese

Effects of Different Potassium Nitrate Concentrations and Temperature Conditions on the Elemental Composition and Blossom-End Rot in Paprika (Capsicum annuum L.)
Ryota Arakawa, Hisae Fujimoto, Haruka Kameoka, Seiro Toriyama, Yoshihiro Yoshida, Toshihiro Watanabe, Hayato Maruyama
The Horticulture Journal, Japanese Society for Horticultural Science, 2021, [Peer-reviewed]
English, Scientific journal,

Blossom-end rot (BER) in paprika (Capsicum annuum L.), as in tomato fruits, is a physiological disorder caused by calcium (Ca) deficiency in fruits. While studies have revealed that excessive nitrogen and potassium fertilization and high temperatures affect Ca transport to fruits and induce BER, few studies have investigated the effects of other elemental concentrations in paprika fruit and their association with BER occurrence. This study aimed to investigate the relationship between the changes in the elemental composition and incidence of BER in paprika fruits grown at different potassium nitrate (KNO₃) concentrations and different temperature conditions. Paprika plants were grown in rockwool blocks in a greenhouse at 25°C and 30°C for approximately four months and irrigated with liquid fertilizer and three different concentrations (0, 27.9, and 74.4 mM) of KNO₃ were added. Subsequently, the total fresh weights of ripe paprika fruits and the incidence of BER were measured, and the elemental compositions in the pericarp of the first and last sampled fruits were analyzed. The total fresh weights significantly decreased and the incidence of BER increased with increasing KNO₃ concentrations and temperature. The profiles of 11 mineral elements in the pericarps of paprika fruits revealed a significant positive or negative correlation between not only the concentration of Ca, but also that of several other elements including boron (which crosslinks pectin in the cell wall like Ca) and the incidence of BER. These results suggest that Ca deficiency may not be the only cause of BER occurrence, and that several elements may also be involved. The insights from this study will contribute to help predict the incidence of BER and stabilize crop production by improving fertilizer application and environmental control.

Expression genome-wide association study identifies that phosphatidylinositol-derived signalling regulates ALUMINIUM SENSITIVE3 expression under aluminium stress in the shoots of Arabidopsis thaliana.
Ayan Sadhukhan, Raj Kishan Agrahari, Liujie Wu, Toshihiro Watanabe, Yuki Nakano, Sanjib Kumar Panda, Hiroyuki Koyama, Yuriko Kobayashi
Plant science : an international journal of experimental plant biology, 302, 110711, 110711, Jan. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, To identify unknown regulatory mechanisms leading to aluminium (Al)-induction of the Al tolerance gene ALS3, we conducted an expression genome-wide association study (eGWAS) for ALS3 in the shoots of 95 Arabidopsis thaliana accessions in the presence of Al. The eGWAS was conducted using a mixed linear model with 145,940 genome-wide single nucleotide polymorphisms (SNPs) and the association results were validated using reverse genetics. We found that many SNPs from the eGWAS were associated with genes related to phosphatidylinositol metabolism as well as stress signal transduction, including Ca2+signals, inter-connected in a co-expression network. Of these, PLC9, CDPK32, ANAC071, DIR1, and a hypothetical protein (AT4G10470) possessed amino acid sequence/ gene expression level polymorphisms that were significantly associated with ALS3 expression level variation. Furthermore, T-DNA insertion mutants of PLC9, CDPK32, and ANAC071 suppressed shoot ALS3 expression in the presence of Al. This study clarified the regulatory mechanisms of ALS3 expression in the shoot and provided genetic evidence of the involvement of phosphatidylinositol-derived signal transduction under Al stress.

Ionomic Responses of Local Plant Species to Natural Edaphic Mineral Variations.
Chengming Zhang, Syuntaro Hiradate, Yoshinobu Kusumoto, Sayaka Morita, Tomoyo F Koyanagi, Qingnan Chu, Toshihiro Watanabe
Frontiers in plant science, 12, 614613, 614613, 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Leaf ionome indicates plant phylogenetic evolution and responses to environmental stress, which is a critical influential factor to the structure of species populations in local edaphic sites. However, little is known about leaf ionomic responses of local plant species to natural edaphic mineral variations. In the present study, all plant species and soil samples from a total of 80 soil sites in Shiozuka Highland were collected for multi-elemental analysis. Ioniomic data of species were used for statistical analysis, representing 24 species and 10 families. Specific preferences to ionomic accumulation in plants were obviously affected by the phylogeny, whereas edaphic impacts were also strong but limited within the phylogenetic preset. Correlations among elements resulted from not only elemental synergy and competition but also the adaptive evolution to withstand environmental stresses. Furthermore, ionomic differences of plant families were mainly derived from non-essential elements. The majority of variations in leaf ionome is undoubtedly regulated by evolutionary factors, but externalities, especially environmental stresses also have an important regulating function for landscape formation, determining that the contributions of each factor to ionomic variations of plant species for adaptation to environmental stress provides a new insight for further research on ionomic responses of ecological speciation to environmental perturbations and their corresponding adaptive evolutions.

Effects of Different Feedstock Type and Carbonization Temperature of Biochar on Oat Growth and Nitrogen Uptake in Coapplication with Compost
Shoki Ochiai, Kazunori Iwabuchi, Takanori Itoh, Toshihiro Watanabe, Mitsuru Osaki, Katsumori Taniguro
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, 21, 1, 276, 285, Oct. 2020
English, Scientific journal

Heterophyllous Shoots of Japanese Larch Trees: The Seasonal and Yearly Variation in CO2 Assimilation Capacity of the Canopy Top with Changing Environment.
Satoshi Kitaoka, Qu Laiye, Yoko Watanabe, Makoto Watanabe, Toshihiro Watanabe, Takayoshi Koike
Plants (Basel, Switzerland), 9, 10, 28 Sep. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Japanese larch (Larix kaempferi = L. leptolepis) is often characterized by its high growth rate with heterophyllous shoots, but the functional differences of heterophyllous shoots still remain unclear. Recently, abrupt high temperature and drought during spring induced high photosynthetic rate via change in leaf morphology of the deciduous habit. In order to reveal the photosynthetic characteristics of both short and long-shoot needles of sunny canopy of the larch trees using a canopy tower, we calculated the seasonal change of gas exchange characters and leaf mass per area (LMA) and foliar nitrogen content (N) of heterophyllous needles: short and long-shoot needles over 3 years. No marked difference in light-saturated photosynthetic rates (Psat) was observed between short and long shoots after leaf maturation to yellowing, although the difference was obvious in a specific year, which only shows that seasonal change in temperature and soil moisture determines the in situ photosynthetic capacity of needles. The large annual and seasonal variations in Psat in both shoots were found to be mainly determined by climatic variations, while shoot types determined the strategy of their photosynthetic N utilization as well as the stomatal regulation.

4-1-35 中国地方の貧栄養な花崗岩質土壌に生育する木本植物の養分吸収特性（4-1 植物の多量栄養素 2020年度岡山大会）
和崎 淳, 岡村 惟史, 山田 大綱, 愛原 健司, 坪田 博美, 渡部 敏裕
日本土壌肥料学会講演要旨集, 66, 48, 48, 一般社団法人 日本土壌肥料学会, 01 Sep. 2020
Japanese

Evaluating Soil–Root Interaction of Hybrid Larch Seedlings Planted under Soil Compaction and Nitrogen Loading
Tetsuto Sugai, Satoko Yokoyama, Yutaka Tamai, Hirotaka Mori, Enrico Marchi, Toshihiro Watanabe, Fuyuki Satoh, Takayoshi Koike
Forests, 11, 9, 947, 947, MDPI AG, 29 Aug. 2020
Scientific journal, Although compacted soil can be recovered through root development of planted seedlings, the relationship between root morphologies and soil physical properties remain unclear. We investigated the impacts of soil compaction on planted hybrid larch F1 (Larix gmelinii var. japonica×L. kaempferi, hereafter F1) seedlings with/without N loading. We assumed that N loading might increase the fine root proportion of F1 seedlings under soil compaction, resulting in less effects of root development on soil recovery. We established experimental site with different levels of soil compaction and N loading, where two-year-old F1 seedlings were planted. We used a hardness change index (HCI) to quantify a degree of soil hardness change at each depth. We evaluated root morphological responses to soil compaction and N loading, focusing on ectomycorrhizal symbiosis. High soil hardness reduced the total dry mass of F1 seedlings by more than 30%. Significant positive correlations were found between HCI and root proportion, which indicated that F1 seedling could enhance soil recovery via root development. The reduction of fine root density and its proportion due to soil compaction was observed, while these responses were contrasting under N loading. Nevertheless, the relationships between HCI and root proportion were not changed by N loading. The relative abundance of the larch-specific ectomycorrhizal fungi under soil compaction was increased by N loading. We concluded that the root development of F1 seedling accelerates soil recovery, where N loading could induce root morphological changes under soil compaction, resulting in the persistent relationship between root development and soil recovery.

Effect of mixed cropping with lupin (Lupinus albus L.) on growth and nitrogen uptake in pasture grasses grown under manure application
Toshihiro Watanabe
ARCHIVES OF AGRONOMY AND SOIL SCIENCE, 66, 1, 96, 109, 02 Jan. 2020, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

14 土壌窒素バランスに対する小麦の葉位別イオノーム解析と光合成機能の関連（北海道支部講演会，2019年度各支部会）
菅井 徹人, 丸山 隼人, 渡部 敏裕, 信濃 卓郎
日本土壌肥料学会講演要旨集, 66, 197, 197, 一般社団法人 日本土壌肥料学会, 2020
Japanese

13 白花ルーピンのK 欠乏下における根圏土壌中K 及びCs の形態変化（北海道支部講演会，2019年度各支部会）
藤本 久恵, 高雄 惇英, 丸山 隼人, 俵谷 圭太郎, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 66, 197, 197, 一般社団法人 日本土壌肥料学会, 2020
Japanese

17 ハーブの養分欠乏ストレスと機能性成分とのかかわり（北海道支部講演会，2019年度各支部会）
伊東 和輝, 渡部 敏裕, 信濃 卓郎, 丸山 隼人
日本土壌肥料学会講演要旨集, 66, 198, 198, 一般社団法人 日本土壌肥料学会, 2020
Japanese

18 ナトリウムによるテンサイの生育促進機構に関する研究（北海道支部講演会，2019年度各支部会）
小濱 諒太, 合田 健登, 丸山 隼人, 菅井 徹人, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 66, 198, 198, 一般社団法人 日本土壌肥料学会, 2020
Japanese

4-1-24 コムギコアコレクションを用いた根圏リンの形態別評価とリン獲得形質との関係（4-1 植物の多量栄養素 2020年度岡山大会）
青山 奈央, 森田 洸介, 渡部 敏裕, 信濃 卓郎, 丸山 隼人
日本土壌肥料学会講演要旨集, 66, 44, 44, 一般社団法人 日本土壌肥料学会, 2020
Japanese

4-1-23 土壌リン・窒素制限下におけるダイズの生育とリン獲得経路の経時的評価（4-1 植物の多量栄養素 2020年度岡山大会）
浅尾 真結子, 渡部 敏裕, 信濃 卓郎, 丸山 隼人
日本土壌肥料学会講演要旨集, 66, 44, 44, 一般社団法人 日本土壌肥料学会, 2020
Japanese

4-3-22 ナトリウムによるテンサイの生育促進と光環境の関係（4-3 植物の有害元素 2020年度岡山大会）
小濱 諒太, 合田 健登, 丸山 隼人, 菅井 徹人, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 66, 68, 68, 一般社団法人 日本土壌肥料学会, 2020
Japanese

4-3-14 火山性強酸性土壌に生育するツクシテンツキのイオノミクス解析（4-3 植物の有害元素 2020年度岡山大会）
藤原 久貴, 丸山 隼人, 和崎 淳, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 66, 65, 65, 一般社団法人 日本土壌肥料学会, 2020
Japanese

4-3-10 アルミニウム集積植物 Melastoma marabathricum におけるMATE 遺伝子の機能解析（4-3 植物の有害元素 2020年度岡山大会）
坂口 文香, 吉井 健祐, 丸山 隼人, 佐々木 孝行, 西田 翔, 和崎 淳, 信濃 卓郎, 渡部 敏裕
日本土壌肥料学会講演要旨集, 66, 64, 64, 一般社団法人 日本土壌肥料学会, 2020
Japanese

Does ozone alter the attractiveness of Japanese white birch leaves to a leaf beetle (Agelastica coerulea) via changes in biogenic volatile organic compounds (BVOCs): An examination with the Y-tube test
Masui N, T Mochizuki, A Tani, H Matsuura, E Agathokleous, T Watanabe, T Koike
Forests, special issue "Impacts of O3, 1, https://www.mdpi.com/1999-4907, 58, MDPI AG, Jan. 2020, [Peer-reviewed]
English, Scientific journal, Elevated ground-level ozone (O3) reduced C-based defense chemicals; however, severe grazing damages were found in leaves grown in the low O3 condition of a free air O3-concentration enrichment (O3-FACE) system. To explain this phenomenon, this study investigates the role of BVOCs (biogenic volatile organic compounds) as signaling compounds for insect herbivores. BVOCs act as scents for herbivore insects to locate host plants, while some BVOCs show high reactivity to O3, inducing changes in the composition of BVOCs in atmospheres with elevated O3. To assess the aforementioned phenomenon, profiles of BVOCs emitted from birch (Betula platyphylla var. japonica Hara) leaves were analyzed ex situ, and Y-tube insect preference tests were conducted in vitro to study the insect olfactory response. The assays were conducted in June and August or September, according to the life cycle of the adult alder leaf beetle Agelastica coerulea Baly (Coleoptera: Chrysomelidae). The Y-tube tests revealed that the leaf beetles were attracted to BVOCs, and O3 per se had neither an attractant nor a repellent effect. BVOCs became less attractant when mixed with highly concentrated O3 (>80 ppb). About 20% of the total BVOCs emitted were highly O3-reactive compounds, such as β-ocimene. The results suggest that BVOCs emitted from the birch leaves can be altered by elevated O3, thus potentially reducing the attractiveness of leaves to herbivorous insects searching for food.

Brief experimental history of evaluation of the environmental environment (elevated CO2 and O3) on urban and suburb trees with use of a mini-FACE and flux monitoring towers
小池, 孝良, 江口, 則和, 渡部, 敏裕, 市川, 一, 藤戸, 永志, 曲, 来葉, 渡辺, 誠, 渡邊, 陽子, アガトクレオス, エフゲニオス, 石, 聡, 北尾, 光俊, 高木, 健太郎, 日浦, 勉, 里村, 多賀美, 半, 智史, 船田, 良, 山﨑, 友紀, 佐藤, 冬樹
Technical report for boreal forest conservation of the Hokkaido University Forests, 37, 18, 26, 北海道大学北方生物圏フィールド科学センター森林圏ステーション, Dec. 2019
Japanese, Research institution

Metabolic reprogramming in nodules, roots, and leaves of symbiotic soybean in response to iron deficiency.
Qingnan Chu, Zhimin Sha, Hayato Maruyama, Linzhang Yang, Gang Pan, Lihong Xue, Toshihiro Watanabe
Plant, cell & environment, 42, 11, 3027, 3043, Nov. 2019, [Peer-reviewed], [Last author], [International Magazine]
English, To elucidate the mechanism of adaptation of leguminous plants to iron (Fe)-deficient environment, comprehensive analyses of soybean (Glycine max) plants (sampled at anthesis) were conducted under Fe-sufficient control and Fe-deficient treatment using metabolomic and physiological approach. Our results show that soybeans grown under Fe-deficient conditions showed lower nitrogen (N) fixation efficiency; however, ureides increased in different tissues, indicating potential N-feedback inhibition. N assimilation was inhibited as observed in the repressed amino acids biosynthesis and reduced proteins in roots and nodules. In Fe-deficient leaves, many amino acids increased, accompanied by the reduction of malate, fumarate, succinate, and α-ketoglutarate, which implies the N reprogramming was stimulated by the anaplerotic pathway. Accordingly, many organic acids increased in roots and nodules; however, enzymes involved in the related metabolic pathway (e.g., Krebs cycle) showed opposite activity between roots and nodules, indicative of different mechanisms. Sugars increased or maintained at constant level in different tissues under Fe deficiency, which probably relates to oxidative stress, cell wall damage, and feedback regulation. Increased ascorbate, nicotinate, raffinose, galactinol, and proline in different tissues possibly helped resist the oxidative stress induced by Fe deficiency. Overall, Fe deficiency induced the coordinated metabolic reprogramming in different tissues of symbiotic soybean plants.

A Reduced Phosphorus Application Rate Using a Mycorrhizal Plant as the Preceding Crop Maintains Soybean Seeds' Nutritional Quality.
Zhimin Sha, Toshihiro Watanabe, Qingnan Chu, Norikuni Oka, Mitsuru Osaki, Takuro Shinano
Journal of agricultural and food chemistry, 67, 1, 32, 42, 09 Jan. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, We tested whether introducing an arbuscular mycorrhizal fungi (AMF)-host plant with a reduced P application rate could maintain soybean seeds' nutrient quality. The dynamic variation of 14 nutrients was analyzed in source and sink organs during the seed-filling stage. The AMF-host and non-AMF-host plants, sunflower and mustard, were grown as preceding crops (PCs). Soybeans, the succeeding crops, were planted with three different phosphorus levels, namely, 0, 50, and 150 kg P2O5 ha-1. The results showed that the AMF-host PC with a reduced P application rate maintained the seed's yield and nutrients quality. During the seed-filling stage, the AMF-host PC with a reduced P application rate increased the uptake of most nutrients compared to the non-AMF-host PC, and improved the remobilization efficiency of all nutrients except Mn, Fe, and Se, compared to the optimal P application rate. These results could help improve the utilization efficiency of P fertilizers and protect soybeans' nutritional value.

Can needle nitrogen content explain the interspecific difference in ozone sensitivities of photosynthesis between Japanese larch (Larix kaempferi) and Sakhalin fir (Abies sachalinensis)?.
T. Sugai, M. Kitao, T. Watanabe, T. Koike
Photosynthetica, 57, 2, 540, 547, Institute of Experimental Botany, 2019, [Peer-reviewed]
Scientific journal

Nitrogen loading increases the ozone sensitivity of larch seedlings with higher sensitivity to nitrogen loadin
Tetsuto Sugai, Toshihiro Watanabe, Kazuhito Kita, Takayoshi Koike
Science of The Total Environment, 663, 1, 587, 595, Elsevier BV, 2019, [Peer-reviewed]
English, Scientific journal

Sensitive to Proton Rhizotoxicity1 Regulates Salt and Drought Tolerance of Arabidopsis thaliana through Transcriptional Regulation of CIPK23
Toshihiro Watanabe
PLANT AND CELL PHYSIOLOGY, 60, 9, 2113, 2126, 2019, [Peer-reviewed]
English, Scientific journal

Salt Stress Reduced the Seedling Growth of Two Larch Species Under Elevated Ozone
Tetsuto Sugai, Wang Yannan, Toshihiro Watanabe, Fuyuki Satoh, Laiye Qu, Takayoshi Koike
Frontiers in Forests and Global Change, 2, 53, Frontiers Media SA, 2019, [Peer-reviewed]
English, Scientific journal

Comparison of the Ability of Several White-rot Fungi to Biobleach Acacia Oxygen-delignified Kraft Pulp
Sitompul Afrida, Toshihiro Watanabe, Yutaka Tamai
Asian Journal of Biotechnology and Bioresource Technology, 5, 3, 1, 10, Sciencedomain International, 2019, [Peer-reviewed]
Scientific journal, Previous screening analyses demonstrated that the in vivo biobleaching activities of the white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 are higher than those of Phanerochaete chrysosporium and Trametes versicolor. The purpose of the current study was to examine the production of extracellular enzymes of these four white-rot fungi grown on three types of low-cost media containing agricultural and forestry waste, and to evaluate the ability of the produced extracellular enzymes to biobleach Acacia oxygen-delignified kraft pulp (A-OKP). The biobleaching activity of extracellular fractions of I. lacteus, L. tigrinus, T. versicolor, and P. chrysosporium cultures was the most pronounced after 3 days of incubation with Acacia mangium wood powder supplemented with rice bran and 1% glucose (WRBG) with resultant Kappa number reduction of 4.4%, 6.7%, 3.3%, and 3.3%, respectively. Therefore, biobleaching ability of I. lacteus and L. tigrinus have been shown to be higher than of T. versicolor and P. chrysosporium, both in vivo and in vitro.

Dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols.
Tadao Wagatsuma, Eriko Maejima, Toshihiro Watanabe, Tomonobu Toyomasu, Masaharu Kuroda, Toshiya Muranaka, Kiyoshi Ohyama, Akifumi Ishikawa, Masami Usui, Shahadat Hossain Khan, Hayato Maruyama, Keitaro Tawaraya, Yuriko Kobayashi, Hiroyuki Koyama
Journal of experimental botany, 69, 3, 567, 577, 23 Jan. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Aluminum-sensitive rice (Oryza sativa L.) cultivars showed increased Al tolerance under dark conditions, because less Al accumulated in the root tips (1 cm) under dark than under light conditions. Under dark conditions, the root tip concentration of total sterols, which generally reduce plasma membrane permeabilization, was higher in the most Al-sensitive japonica cultivar, Koshihikari (Ko), than in the most Al-tolerant cultivar, Rikuu-132 (R132), but the phospholipid content did not differ between the two. The Al treatment increased the proportion of stigmasterol (which has no ability to reduce membrane permeabilization) out of total sterols similarly in both cultivars under light conditions, but it decreased more in Ko under dark conditions. The carotenoid content in the root tip of Al-treated Ko was significantly lower under dark than under light conditions, indicating that isopentenyl diphosphate transport from the cytosol to plastids was decreased under dark conditions. HMG2 and HMG3 (encoding the key sterol biosynthetic enzyme 3-hydroxy-3-methylglutaryl CoA reductase) transcript levels in the root tips were enhanced under dark conditions. We suggest that the following mechanisms contribute to the increase in Al tolerance under dark conditions: inhibition of stigmasterol formation to retain membrane integrity; greater partitioning of isopentenyl diphosphate for sterol biosynthesis; and enhanced expression of HMGs to increase sterol biosynthesis.

Effects of ozone (O3) and ethylenediurea (EDU) on the ecological stoichiometry of a willow grown in a free-air exposure system
Evgenios Agathokleous, Mitsutoshi Kitao, Chu Qingnan, Costas J Saitanis, Elena Paoletti, William J Manning, Toshihiro Watanabe, Takayoshi Koike
Environmental Pollution, 238, 663, 676, 2018, [Peer-reviewed]
English, Scientific journal

Concentration of some trace elements in two wild edible ferns, Diplazium esculentum and Stenochlaena palutris, inhabiting tropical peatlands under different environments in Central Kalimantan
Rahmawati Della, Wijaya C. Hanny, Hashidoko Yasuyuki, Djajakirana Gunawan, Haraguchi Akira, Watanabe Toshihiro, Kuramochi Kanta, Nion Yanetri Asi
Eurasian Journal of Forest Research, 20, 11, 20, Hokkaido University Forests, EFRC, Dec. 2017, [Peer-reviewed]
English, Research institution, Young leaves of two wild ferns (Diplazium esculentum and Stenochlaena palutris) are uniquely eaten among local peoples in Kalimantan Island. These edible ferns are regarded as important sources of Fe and other trace elements essential for human body. In order to investigate the effect of soil environments on the metal content, we analyzed eight elements (Al, Fe, Cr, Cu, Mn, Zn, Cs, and Pb) in the edible ferns collected from five different sites (riverside of Kahayan River near Bukit Rawi, farming area in Tangkiling, natural forest near Kasongan, and two peat soils at campus of The University of Palangkaraya and residential area in Palangkaraya city) in Central Kalimantan with distinguishable soil conditions. At the five sampling locations, we also collected an aluminum accumulator bush tree (Melastoma malabathricum) as a reference plant. The underground parts of S. palutris and D. esculentum accumulated higher concentration of Al and Fe than their leaf parts, whereas M. malabathricum was rich in Al and Mn in the leaves rather than the roots. Although Fe contents in the edible parts of D. esculentum (3.1–3.3 μg/g-dry weight of young leaves) and S. palutris (1.0–2.1 μg/g dw leaves) from the samples collected at the riverside and the farming area were not so high, Al contents in their edible parts (3.9–6.3 and 7.8–7.9 μg/g-dry weight, respectively) were not excessive. Other trace elements, including Mn, was not the levels to be concerned. Thus, utilizing young leaves and shoots of the ferns as wild vegetable seemed to cover chronical deficiency of trace elements necessary for human nutrition, without any excessive intake of Al and other toxic metals, unless collected at urban area. We further found relatively high Cs-absorbable cropping plants in this study.

LjMOT1, a high-affinity molybdate transporter from Lotus japonicus, is essential for molybdate uptake, but not for the delivery to nodules.
Guilan Duan, Tsuneo Hakoyama, Takehiro Kamiya, Hiroki Miwa, Fabien Lombardo, Shusei Sato, Satoshi Tabata, Zheng Chen, Toshihiro Watanabe, Takuro Shinano, Toru Fujiwara
The Plant journal : for cell and molecular biology, 90, 6, 1108, 1119, Jun. 2017, [Peer-reviewed], [International Magazine]
English, Molybdenum (Mo) is an essential nutrient for plants, and is required for nitrogenase activity of legumes. However, the pathways of Mo uptake from soils and then delivery to the nodules have not been characterized in legumes. In this study, we characterized a high-affinity Mo transporter (LjMOT1) from Lotus japonicus. Mo concentrations in an ethyl methanesulfonate-mutagenized line (ljmot1) decreased by 70-95% compared with wild-type (WT). By comparing the DNA sequences of four AtMOT1 homologs between mutant and WT lines, one point mutation was found in LjMOT1, which altered Trp292 to a stop codon; no mutation was found in the other homologous genes. The phenotype of Mo concentrations in F2 progeny from ljmot1 and WT crosses were associated with genotypes of LjMOT1. Introduction of endogenous LjMOT1 to ljmot1 restored Mo accumulation to approximately 60-70% of the WT. Yeast expressing LjMOT1 exhibited high Mo uptake activity, and the Km was 182 nm. LjMOT1 was expressed mainly in roots, and its expression was not affected by Mo supply or rhizobium inoculation. Although Mo accumulation in the nodules of ljmot1 was significantly lower than that of WT, it was still high enough for normal nodulation and nitrogenase activity, even for cotyledons-removed ljmot1 plants grown under low Mo conditions, in this case the plant growth was significantly inhibited by Mo deficiency. Our results suggest that LjMOT1 is an essential Mo transporter in L. japonicus for Mo uptake from the soil and growth, but is not for Mo delivery to the nodules.

Leaf stoichiometry of deciduous tree species in different soils exposed to free-air O-3 enrichment over two growing seasons
Cong Shi, Toshihiro Watanabe, Takayoshi Koike
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 138, 148, 163, Jun. 2017, [Peer-reviewed]
English, Scientific journal

The evolution of aluminum accumulation in ferns and lycophytes
Marco Schmitt, Klaus Mehltreter, Michael Sundue, Weston Testo, Toshihiro Watanabe, Steven Jansen
AMERICAN JOURNAL OF BOTANY, 104, 4, 573, 583, Apr. 2017, [Peer-reviewed]
English, Scientific journal

Contrasting Effects of Cattle Manure Applications and Root-Induced Changes on Heavy Metal Dynamics in the Rhizosphere of Soybean in an Acidic Haplic Fluvisol: A Chronological Pot Experiment
Qingnan Chu, Zhimin Sha, Mitsuru Osaki, Toshihiro Watanabe
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 65, 15, 3085, 3095, Apr. 2017, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Contribution of constitutive characteristics of lipids and phenolics in roots of tree species in Myrtales to aluminum tolerance
Toshihiro Watanabe
PHYSIOLOGIA PLANTARUM, 160, 1, 11, 20, 2017
Scientific journal

A Pronounced Improvement of the Kappa Number Reduction and Pulp Properties Associated with the Use of Extracellular Enzymes Secreted by Selected Fungal Strains
Toshihiro Watanabe
BIORESOURCES, 12, 4, 8272, 8285, 2017, [Peer-reviewed]
Scientific journal

Differential Responses of Soybean and Sorghum Growth, Nitrogen Uptake, and Microbial Metabolism in the Rhizosphere to Cattle Manure Application: A Rhizobox Study
Qingnan Chu, Zhimin Sha, Takuji Nakamura, Norikuni Oka, Mitsuru Osaki, Toshihiro Watanabe
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 64, 43, 8084, 8094, Nov. 2016, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Retranslocation of foliar nutrients of deciduous tree seedlings in different soil condition under free-air O-3 enrichment
Cong Shi, Norikazu Eguchi, Fankang Meng, Toshihiro Watanabe, Fuyuki Satoh, Takayoshi Koike
IFOREST-BIOGEOSCIENCES AND FORESTRY, 9, 5, 835, 841, Oct. 2016, [Peer-reviewed]
English, Scientific journal

The dynamic state of the ionome in roots, nodules, and shoots of soybean under different nitrogen status and at different growth stages
Qingnan Chu, Toshihiro Watanabe, Takuro Shinano, Takuji Nakamura, Norikuni Oka, Mitsuru Osaki, Zhimin Sha
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, 179, 4, 488, 498, Aug. 2016
English, Scientific journal

Distribution of minerals in young and mature leaves of different leaf vegetable crops cultivated in a field.　　　　　　　　　　　　　　　
Watanabe, T, Maejima, E, Urayama, M, Owadano, M, Yamauchi, A, Okada, R, Shinano, T, Osaki, M
Open Journal of Plant Science, 1, 1, 5, 9, May 2016, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Metabolomics approach in soybean　　　　　　　　　　　　　　　
Takuji Nakamura, Keiki Okazaki, Noureddine Benkeblia, Jun Wasaki, Toshihiro Watanabe, Hideyuki Matsuura, Hirofumi Uchimiya, Setsuko Komatsu, Takuro Shinano
Genetics, Genomics, and Breeding of Soybean, 313, 330, CRC Press, 19 Apr. 2016
English, In book

Aluminium Accumulation and Intra-Tree Distribution Patterns in Three Arbor aluminosa (Symplocos) Species from Central Sulawesi
Marco Schmitt, Sven Boras, Aiyen Tjoa, Toshihiro Watanabe, Steven Jansen
PLOS ONE, 11, 2, e0149078, Feb. 2016
English, Scientific journal

ニホンカラマツとグイマツ雑種F1苗木の光合成能に対するオゾン暴露と硫酸アンモニウム付加の影響
菅井徹人, 渡部敏裕, 来田和人, 齋藤秀之, 渋谷正人, 小池孝良
北方森林研究, 64, 64, 63, 66, 北方森林学会, Feb. 2016, [Peer-reviewed]
Japanese

The Ionomic Study of Vegetable Crops.
Toshihiro Watanabe, Eriko Maejima, Tomoko Yoshimura, Masaru Urayama, Aiko Yamauchi, Masako Owadano, Ryosuke Okada, Mitsuru Osaki, Yoshinori Kanayama, Takuro Shinano
PloS one, 11, 8, e0160273, 2016, [International Magazine]
English, Soil contains various essential and nonessential elements, all of which can be absorbed by plants. Plant ionomics is the study of the accumulation of these elements (the ionome) in plants. The ionomic profile of a plant is affected by various factors, including species, variety, organ, and environment. In this study, we cultivated various vegetable crop species and cultivars under the same field conditions and analyzed the level of accumulation of each element in the edible and nonedible parts using ionomic techniques. The concentration of each element in the edible parts differed between species, which could be partly explained by differences in the types of edible organs (root, leaf, seed, and fruit). For example, the calcium concentration was lower in seeds and fruit than in other organs because of the higher dependency of calcium accumulation on xylem transfer. The concentration of several essential microelements and nonessential elements in the edible parts also varied greatly between cultivars of the same species, knowledge of which will help in the breeding of vegetables that are biofortified or contain lower concentrations of toxic elements. Comparison of the ionomes of the fruit and leaves of tomato (Solanum lycopersicum) and eggplant (S. melongena) indicated that cadmium and boron had higher levels of accumulation in eggplant fruit, likely because of their effective transport in the phloem. We also found that homologous elements that have been reported to share the same uptake/transport system often showed significant correlation only in a few families and that the slopes of these relationships differed between families. Therefore, these differences in the characteristics of mineral accumulation are likely to affect the ionomic profiles of different families.

Effect of biochar application on mineral and microbial properties of soils growing different plant species
Anna Hairani, Mitsuru Osaki, Toshihiro Watanabe
SOIL SCIENCE AND PLANT NUTRITION, 62, 5-6, 519, 525, 2016
English, Scientific journal

The effects of aluminium on plant growth in a temperate and deciduous aluminium accumulating species
Marco Schmitt, Toshihiro Watanabe, Steven Jansen
AOB PLANTS, 8, plw065, 2016
English, Scientific journal

Effects of ozone and/or ammonium sulfate on the photosynthesis and growth of Japanese larch and hybrid larch F₁
Sugai Tetsuto, Watanabe Toshihiro, Kita Kazuhito, Saito Hideyuki, Shibuya Masato, Koike Takayoshi
The Japanese Forest Society Congress, 127, 730, 730, THE JAPANESE FORESTRY SOCIETY, 2016
Japanese, [in Japanese]

4-3-10 低温条件下で栽培したMelastoma malabathricumの生育に対するアルミニウムの効果（4-3 植物の有害元素，2016年度佐賀大会）
前島 恵理子, 合田 健登, 大崎 満, 渡部 敏裕
日本土壌肥料学会講演要旨集, 62, 66, 66, 一般社団法人 日本土壌肥料学会, 2016
Japanese

4-3-4 Effect of cattle farmyard manure application on heavy metals dynamics in soil and soybean plant（4-3 植物の有害元素，2016年度佐賀大会）
褚 清南, 渡部 敏裕, 中村 卓司, 岡 紀邦, 大崎 滿
日本土壌肥料学会講演要旨集, 62, 64, 64, 一般社団法人 日本土壌肥料学会, 2016
Japanese

7-2-2 テンサイの擂潰籾殻混合培地が移植後の生育に及ぼす影響（7-2 土壌改良資材，2016年度佐賀大会）
大竹 勝, 渡部 敏裕, 大崎 満
日本土壌肥料学会講演要旨集, 62, 137, 137, 一般社団法人 日本土壌肥料学会, 2016
Japanese

P4-3-7 ストレス条件下でのテンサイの生育に対するナトリウムの効果（ポスター，4-3 植物の有害元素，2016年度佐賀大会）
南波佐間 幹人, 前島 恵理子, 松本 卓也, 土谷 修平, 大崎 満, 渡部 敏裕
日本土壌肥料学会講演要旨集, 62, 73, 73, 一般社団法人 日本土壌肥料学会, 2016
Japanese

P4-2-3 RILsを用いた大豆青立ち耐性機構の解明 1. 包括的元素分析による解析（ポスター，4-2 植物の微量栄養素，2016年度佐賀大会）
中村 卓司, 山田 哲也, 渡部 敏裕, 岡崎 圭毅
日本土壌肥料学会講演要旨集, 62, 61, 61, 一般社団法人 日本土壌肥料学会, 2016
Japanese

14 テンサイの擂潰籾殻混合育苗培地がペーパーポット紙質へ及ばす影響（北海道支部講演会，2015年度各支部会）
大竹 勝, 渡部 敏裕, 大崎 満
日本土壌肥料学会講演要旨集, 62, 243, 243, 一般社団法人 日本土壌肥料学会, 2016
Japanese

12 シロバナルーピンで観察される特異的なCs 吸収傾向（北海道支部講演会，2015年度各支部会）
高雄 惇英, 渡部 敏裕, 和崎 淳, 大崎 満
日本土壌肥料学会講演要旨集, 62, 242, 242, 一般社団法人 日本土壌肥料学会, 2016
Japanese

13 テンサイのカリウム施肥、リン酸施肥及び栽植密度の変動が収量・品質に及ぼす影響の場所間差と土壌分析値との関連について（北海道支部講演会，2015年度各支部会）
鷹田 秀一, 渡部 敏裕, 大崎 満
日本土壌肥料学会講演要旨集, 62, 243, 243, 一般社団法人 日本土壌肥料学会, 2016
Japanese

IV-1 熱帯アジア地域の問題土壌と農業生産 強酸性土壌に生育する植物とアルミニウムの関係（シンポジウム，2016年度佐賀大会）
渡部 敏裕, 前島 恵理子
日本土壌肥料学会講演要旨集, 62, 206, 206, 一般社団法人 日本土壌肥料学会, 2016
Japanese

8-1-38 Potential effect of wood and bone biochars on greenhouse gas emission of paddy soil under waterlogged and upland conditions（8-1 環境保全，2016年度佐賀大会）
Hairani Anna, 渡部 敏裕, 長竹 新, Nursyamsi Dedi, 波多野 隆介, 大崎 満
日本土壌肥料学会講演要旨集, 62, 155, 155, 一般社団法人 日本土壌肥料学会, 2016
English

7-1-19 テンサイのカリウム施肥、リン酸施肥が収量・品質に及ぼす影響と土壌分析値との関連及び圃場におけるカリウム・りん酸施肥効果特性の判別について（7-1 肥料および施肥法，2016年度佐賀大会）
鷹田 秀一, 渡部 敏裕, 大崎 満
日本土壌肥料学会講演要旨集, 62, 125, 125, 一般社団法人 日本土壌肥料学会, 2016
Japanese

Varietal difference in radiocesium uptake and transfer from radiocesium deposited soils in the genus Amaranthus (vol 60, pg 809, 2014)
Toshihiro Watanabe
SOIL SCIENCE AND PLANT NUTRITION, 62, 5-6, 569, 2016, [Peer-reviewed]
Scientific journal

Ionomic Analysis of Horticultural Plants Reveals Tissue-specific Element Accumulation
Tomoki Shibuya, Toshihiro Watanabe, Hirold Ikeda, Yoshinori Kanayama
HORTICULTURE JOURNAL, 84, 4, 305, 313, Oct. 2015
English, Scientific journal

Interactions between Cs, Sr, and other nutrients and trace element accumulation in Amaranthus shoot in response to variety effect.
Qingnan Chu, Toshihiro Watanabe, Zhimin Sha, Mitsuru Osaki, Takuro Shinano
Journal of agricultural and food chemistry, 63, 8, 2355, 63, 04 Mar. 2015, [International Magazine]
English, Aiming at clarifying the interactions between Cs, Sr, and other mineral elements in the genus Amaranthus, this study adopted 33 different varieties of Amaranthus and investigated the concentrations of 23 mineral elements in shoots grown in the fields of Iino in Fukushima prefecture. Significant varietal effects were detected for all elements except Se, and degree of interspecies variation was highly element dependent. Among 23 elements, amaranths were less sensitive to the accumulation of Cs and Sr than most other mineral elements to the species level. There are six elements showing significant correlation with Cs, positive correlations between As, Rb, Al, Fe, Ni, and Cs, and negative correlation between Ba and Cs. Significant correlations between Ca, Mg, Mn, Zn, B, Ba, Cd, and Sr were detected, and all of the coefficients were positive. Cs and Sr did not present significant correlation, but they were both significantly correlated with Ba. By principal component analysis (PCA), the first and second principal components (PC1 and PC2) accounted for 23.2 and 20.3% of the total variance and associated with Cs and Sr, respectively. Both of the two species took up more Cs by promoting the influx of elements positively correlated with Cs into shoot, but at the same time, Amaranthus hypochondriacus (L.) Mapes 847 decreased the K and Ba uptake and Amaranthus powellii (S. Wats) subsp. Powellii inhibited the accumulation of Rb, Sr, and significantly correlated elements of Sr in shoot. This study is the first to pave the way for comprehension on ionome in amaranth shoot at the variety level. The results of this research provide the ionomic basis for implementing countermeasures in the field against the translocation of Cs (and potentially Sr) toward crops and food.

Higher sterol content regulated by CYP51 with concomitant lower phospholipid content in membranes is a common strategy for aluminium tolerance in several plant species
Tadao Wagatsuma, Md. Shahadat Hossain Khan, Toshihiro Watanabe, Eriko Maejima, Hitoshi Sekimoto, Takao Yokota, Takeshi Nakano, Tomonobu Toyomasu, Keitaro Tawaraya, Hiroyuki Koyama, Matsuo Uemura, Satoru Ishikawa, Takashi Ikka, Akifumi Ishikawa, Takeshi Kawamura, Satoshi Murakami, Nozomi Ueki, Asami Umetsu, Takayuki Kannari
JOURNAL OF EXPERIMENTAL BOTANY, 66, 3, 907, 918, Feb. 2015
English, Scientific journal

Application of ionomics to plant and soil in fields under long-term fertilizer trials.
Toshihiro Watanabe, Masaru Urayama, Takuro Shinano, Ryosuke Okada, Mitsuru Osaki
SpringerPlus, 4, 1, 781, 781, 2015, [International Magazine]
English, Ionomics is the study of elemental accumulation in living organisms using high-throughput elemental profiling. In the present study, we examined the ionomic responses to nutrient deficiency in maize grown in the field in long-term fertilizer trials. Furthermore, the available elements in the field soils were analyzed to investigate their changes under long-term fertilizer treatment and the ionomic relationships between plant and soil. Maize was cultivated in a field with the following five long-term fertilizer treatments: complete fertilization, fertilization without nitrogen, without phosphorus, without potassium, and no fertilization. Concentrations of 22 elements in leaves at an early flowering stage and in soils after harvest were determined. The fertilizer treatments changed the availabilities of many elements in soils. For example, available cesium was decreased by 39 % and increased by 126 % by fertilizations without nitrogen and potassium, respectively. Effects of treatments on the ionome in leaves were evaluated using the translocation ratio (the concentration in leaves relative to the available concentration in soils) for each element. Nitrogen deficiency specifically increased the uptake ability of molybdenum, which might induce the enhancement of nitrogen assimilation and/or endophytic nitrogen fixation in plant. Potassium deficiency drastically enhanced the uptake ability of various cationic elements. These elements might act as alternatives to K in osmoregulation and counterion of organic/inorganic anions. Two major groups of elements were detected by multivariate analyses of plant ionome. Elements in the same group may be linked more or less in uptake and/or translocation systems. No significant correlation between plant and soil was found in concentrations of many elements, even though various soil extraction methods were applied, implying that the interactions between the target and other elements in soil must be considered when analyzing mineral dynamics between plant and soil.

Significant Role of the Plasma Membrane Lipid Bilayers in Aluminum Tolerance of Plants
Toshihiro Watanabe
ALUMINUM STRESS ADAPTATION IN PLANTS, 2015
Scientific journal

Varietal difference in radiocesium uptake and transfer from radiocesium deposited soils in the genus Amaranthus
Takuro Shinano, Toshihiro Watanabe, Qingnan Chu, Mitsuru Osaki, Daisuke Kobayashi, Toshiyasu Okouchi, Hisaya Matsunami, Osamu Nagata, Keiki Okazaki, Takuji Nakamura
SOIL SCIENCE AND PLANT NUTRITION, 60, 6, 809, 817, Nov. 2014
English, Scientific journal

Biobleaching of Acacia kraft pulp with extracellular enzymes secreted by Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 using low-cost media
Sitompul Afrida, Yutaka Tamai, Toshihiro Watanabe, Mitsuru Osaki
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 30, 8, 2263, 2271, Aug. 2014
English, Scientific journal

Rice RCN1/OsABCG5 mutation alters accumulation of essential and nonessential minerals and causes a high Na/K ratio, resulting in a salt-sensitive phenotype
Shuichi Matsuda, Hidetaka Nagasawa, Nobuhiro Yamashiro, Naoko Yasuno, Toshihiro Watanabe, Hideyuki Kitazawa, Sho Takano, Yoshihiko Tokuji, Masayuki Tani, Itsuro Takamure, Kiyoaki Kato
PLANT SCIENCE, 224, 103, 111, Jul. 2014
English, Scientific journal

Comparative analysis of aluminum accumulation in leaves of three angiosperm species
Eriko Maejima, Syuntaro Hiradate, Steven Jansen, Mitsuru Osaki, Toshihiro Watanabe
BOTANY-BOTANIQUE, 92, 5, 327, 331, May 2014
English, Scientific journal

Phosphorus deficiency enhances aluminum tolerance of rice (Oryza sativa) by changing the physicochemical characteristics of root plasma membranes and cell walls
Eriko Maejima, Toshihiro Watanabe, Mitsuru Osaki, Tadao Wagatsuma
JOURNAL OF PLANT PHYSIOLOGY, 171, 2, 9, 15, Jan. 2014
English, Scientific journal

Arsenic alters uptake and distribution of sulphur in Pteris vittata.
Toshihiro Watanabe, Ran Kouho, Tomo Katayose, Nobuyuki Kitajima, Naoya Sakamoto, Noriko Yamaguchi, Takuro Shinano, Hisayoshi Yurimoto, Mitsuru Osaki
Plant, cell & environment, 37, 1, 45, 53, Jan. 2014, [International Magazine]
English, Scientific journal, Low-molecular-weight thiol (LMWT) synthesis has been reported to be directly induced by arsenic (As) in Pteris vittata, an As hyperaccumulator. Sulphur (S) is a critical component of LMWTs. Here, the effect of As treatment on the uptake and distribution of S in P. vittata was investigated. In P. vittata grown under low S conditions, the presence of As in the growth medium enhanced the uptake of SO4(2-), which was used for LMWT synthesis in fronds. In contrast, As application did not affect SO4(2-) uptake in Nephrolepis exaltata, an As non-hyperaccumulator. Moreover, the isotope microscope system revealed that S absorbed with As accumulated locally in a vacuole-like organelle in epidermal cells, whereas S absorbed alone was distributed uniformly. These results suggest that S is involved in As transport and/or accumulation in P. vittata. X-ray absorption near-edge structure analysis revealed that the major As species in the fronds and roots of P. vittata were inorganic As(III) and As(V), respectively, and that As-LMWT complexes occurred as a minor species. Consequently, in case of As accumulation in P. vittata, S possibly acts as a temporary ligand for As in the form of LMWTs in intercellular and/or intracellular transport (e.g. vacuolar sequestration).

Proportion of phospholipids in the plasma membrane is an important factor in Al tolerance
Eriko Maejima, Toshihiro Watanabe
Plant Signaling and Behavior, 9, MAY, e29277, Landes Bioscience, 2014
English, Scientific journal

Comparative analysis of aluminum accumulation in leaves of three angiosperm species
Eriko Maejima, Syuntaro Hiradate, Steven Jansen, Mitsuru Osaki, Toshihiro Watanabe
Botany, 92, 5, 327, 331, National Research Council of Canada, 2014, [Peer-reviewed]
English, Scientific journal

Molecular and Physiological Analysis of Al3+ and H+ Rhizotoxicities at Moderately Acidic Conditions
Yasufumi Kobayashi, Yuriko Kobayashi, Toshihiro Watanabe, Jon E. Shaff, Hiroyuki Ohta, Leon V. Kochian, Tadao Wagatsuma, Thomas B. Kinraide, Hiroyuki Koyama
PLANT PHYSIOLOGY, 163, 1, 180, 192, Sep. 2013
English, Scientific journal

Metabolite Profiling of Mizuna (Brassica rapa L. var. Nipponsinica) To Evaluate the Effects of Organic Matter Amendments
Toshihiro Watanabe
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 61, 5, 1009, 1016, 2013
Scientific journal

Effect of Phosphorus Levels on the Protein Profiles of Secreted Protein and Root Surface Protein of Rice
Takuro Shinano, Tomoko Yosihmura, Toshihiro Watanabe, Yusuke Unno, Mitsuru Osaki, Yohei Nanjo, Setsuko Komatsu
Journal of Proteome Research, 12, 11, 4748, 4756, 2013
English, Scientific journal

Ionome of Soybean Seed Affected by Previous Cropping with Mycorrhizal Plant and Manure Application
Zhimin Sha, Norikuni Oka, Toshihiro Watanabe, Biatna Dulbert Tampubolon, Keiki Okazaki, Mitsuru Osaki, Takuro Shinano
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60, 38, 9543, 9552, Sep. 2012
English, Scientific journal

Effect of exogenous phosphatase and phytase activities on organic phosphate mobilization in soils with different phosphate adsorption capacities
Hayato Maruyama, Takuya Yamamura, Yohei Kaneko, Hirokazu Matsui, Toshihiro Watanabe, Takuro Shinano, Mitsuru Osaki, Jun Wasaki
SOIL SCIENCE AND PLANT NUTRITION, 58, 1, 41, 51, 2012
English, Scientific journal

DIFFERENCES IN ACQUISITION OF ORGANIC NITROGEN IN SOILS BETWEEN BOK CHOY AND TOMATO
Toshihiro Watanabe, Takumi Enomoto, Michihiko Sakurai, Miwa Okamoto, Takuro Shinano, Mitsuru Osaki
JOURNAL OF PLANT NUTRITION, 35, 10, 1468, 1476, 2012
English, Scientific journal

Ionomic response of Lotus japonicus to different root-zone temperatures
Quazi Forhad Quadir, Toshihiro Watanabe, Zheng Chen, Mitsuru Osaki, Takuro Shinano
SOIL SCIENCE AND PLANT NUTRITION, 57, 2, 221, 232, Apr. 2011
English, Scientific journal

Proteomic analysis of secreted proteins from aseptically grown rice
T. Shinano, S. Komatsu, T. Yoshimura, S. Tokutake, F. -J. Kong, T. Watanabe, J. Wasaki, M. Osaki
PHYTOCHEMISTRY, 72, 4-5, 312, 320, Mar. 2011
English, Scientific journal

Changes in saccharide, amino acid and S-methylmethionine content during malting of barley grown with different nitrogen and sulfur status
Masahito Nanamori, Toshihiro Watanabe, Takuro Shinano, Makoto Kihara, Kazumitsu Kawahara, Shinji Yamada, Mitsuru Osaki
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 91, 1, 85, 93, Jan. 2011
English, Scientific journal

Influence of arsenic stress on synthesis and localization of low-molecular-weight thiols in Pteris vittata
Yuki Sakai, Toshihiro Watanabe, Jun Wasaki, Takeshi Senoura, Takuro Shinano, Mitsuru Osaki
ENVIRONMENTAL POLLUTION, 158, 12, 3663, 3669, Dec. 2010
English, Scientific journal

Promotion of root growth by the application of inosine
Dai Tokuhisa, Takuro Shinano, Toshihiro Watanabe, Takuya Yamamura, Mitsuru Osaki
SOIL SCIENCE AND PLANT NUTRITION, 56, 2, 272, 280, Apr. 2010
English, Scientific journal

Screening of white rot fungi for biobleaching of Acacia oxygen-delignified kraft pulp
Sitompul Afrida, Yutaka Tamai, Toshihiro Watanabe, Mitsuru Osaki
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 25, 4, 639, 647, Apr. 2009
English, Scientific journal

Element interconnections in Lotus japonicus: A systematic study of the effects of element additions on different natural variants
Zheng Chen, Toshihiro Watanabe, Takuro Shinano, Tatsuhiro Ezawa, Jun Wasaki, Kazuhiko Kimura, Mitsuru Osaki, Yong-Guan Zhu
SOIL SCIENCE AND PLANT NUTRITION, 55, 1, 91, 101, Feb. 2009
English, Scientific journal

Analysis of Diversity of Diazotrophic Bacteria Associated with the Rhizosphere of a Tropical Arbor, Melastoma malabathricum L.
Atsuya Sato, Toshihiro Watanabe, Yusuke Unno, Erry Purnomo, Mitsuru Osaki, Takuro Shinano
MICROBES AND ENVIRONMENTS, 24, 2, 81, 87, 2009
English, Scientific journal

Mechanisms of Aluminum Tolerance in Phosphoenolpyruvate Carboxylase Transgenic Rice
Toshihiro Watanabe
JOURNAL OF PLANT NUTRITION, 32, 1, 84, 96, 2009
Scientific journal

Rapid characterization of plant mutants with an altered ion-profile: a case study using Lotus japonicus
Zheng Chen, Toshihiro Watanabe, Takuro Shinano, Keiki Okazaki, Mitsuru Osaki
NEW PHYTOLOGIST, 181, 4, 795, 801, 2009
English, Scientific journal

Effect of Zero-Valent Iron Application on Cadmium Uptake in Rice Plants Grown in Cadmium-Contaminated Soils
Toshihiro Watanabe, Yasutoshi Murata, Takashi Nakamura, Yuki Sakai, Mitsuru Osaki
JOURNAL OF PLANT NUTRITION, 32, 7, 1164, 1172, 2009
English, Scientific journal

Amaranthus Tricolor Has the Potential for Phytoremediation of Cadmium-Contaminated Soils
Toshihiro Watanabe, Yasutoshi Murata, Mitsuru Osaki
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS, 40, 19-20, 3158, 3169, 2009
English, Scientific journal

Effects of nitrogen application on malt modification and dimethyl sulfide precursor production in two Japanese barley cultivars
Masahito Nanamori, Ryoichi Kanatani, Makoto Kihara, Kazumitsu Kawahara, Katsuhiro Hayashi, Toshihiro Watanabe, Takuro Shinano, Mitsuru Osaki
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 88, 8, 1464, 1471, Jun. 2008
English, Scientific journal

Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation
Toshihiro Watanabe, Seiji Misawa, Syuntaro Hiradate, Mitsuru Osaki
NEW PHYTOLOGIST, 178, 3, 581, 589, May 2008
English, Scientific journal

Biotransformation of (+)-catechin into taxifolin by a two-step oxidation: Primary stage of (+)-catechin metabolism by a novel (+)-catechin-degrading bacteria, Burkholderia sp KTC-1, isolated from tropical peat
Motoki Matsuda, Yuichiro Otsuka, Shigeki Jin, Jun Wasaki, Jun Watanabe, Toshihiro Watanabe, Mitsuru Osaki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 366, 2, 414, 419, Feb. 2008
English, Scientific journal

Advances in developing screening methods and improving aluminum resistance in common bean and Brachiaria
Idupulapati Rao, Peter Wenzl, Adriana Arango, John Miles, Toshihiro Watanabe, Takuro Shinano, Mitsuru Osaki, Tadao Wagatsuma, Germ?n Manrique, Stephen Beebe, Joe Tohme, Manabu Ishitani, Andr?s Rangel, Walter Horst
Current Agricultural Science and Technology, 14, 4, 1, 7, 2008

Root mucilage enhances aluminum accumulation in Melastoma malabathricum, an aluminum accumulator
Toshihiro Watanabe, Seiji Misawa, Syuntaro Hiradate, Mitsuru Osaki
Plant Signaling and Behavior, 3, 8, 603, 605, Landes Bioscience, 2008
English, Scientific journal

Sago (Metroxylon sagu Rottb.) growth is affected by weeds in a tropical peat swamp in Thailand
Monrawee Yanbuaban, Mitsuru Osaki, Tanit Nuyim, Jumpen Onthong, Toshihiro Watanabe
SOIL SCIENCE AND PLANT NUTRITION, 53, 3, 267, 277, Jun. 2007
English, Scientific journal

Screening of phosphate-solubilizing microorganisms in rhizosphere and rhizoplane of adverse soil-adapting plants in Southern Thailand
Ashara Pengnoo, Yasuyuki Hashidoko, Jumpen Onthong, Sayjai Gimsanguan, Manoon Sae-ong, Takuro Shinano, Toshihiro Watanabe, Mitsuru Osaki
Tropics, 16, 1, 10, 7, 日本熱帯生態学会, 2007
English, Various adverse soils; peat, podzolic sandy, acid sulfate and salted-affected acid sulfate soils were widely distributed throughout Southern Thailand. These soils were infertile and strongly acidic. Phosphorus fertility of these soils was low due to the low content of available phosphorus (0.31-3.70 mg kg-1) especially in podzolic sandy soil. Acid sulfate soil and salt-affected acid sulfate soil contain relatively high level of exchangeable Al (13.3 and 9.87 cmolc kg-1, respectively). However, some native plants; Melaleuca cajuputi, Melastoma malabathricum, Scleria sumatrensis and Oryza sativa grow well in these adverse soils. Since the adverse soils distributed there are phosphorus-unavailable soils. Hence, base on an idea that phosphate-solubilizing microorganism is a key factor for plant growth, such functional rhizospherous microorganisms were screened using modified Pikovskaya,s media containing 5 mg l-1 P in different phosphate forms, Al-PO4, Fe-PO4 and Na-phytate, and adjusted pH 4.0. The number of microorganisms in the rhizosphere tended to be lower than in the rhizoplane. Twelve isolates showed remarkable growth performance on these solid media. Among these selected, 5 strains were obtained from acid sulfate soil, suggesting acid sulfate soil-adapting plant is a good source of the phosphate-solubilizing microorganism.

Nutritional ecology of plants grown in a tropical peat swamp
Monrawee YANBUABAN, Tanit NUYIM, Takeshi MATSUBARA, Toshihiro WATANABE, Mitsuru OSAKI
Tropics, 16, 1, 31, 39, 日本熱帯生態学会, 2007
English

Evolutionary control of leaf element composition in plants
Toshihiro Watanabe, Martin R. Broadley, Steven Jansen, Philip J. White, Jitsuya Takada, Kenichi Satake, Takejiro Takamatsu, Sehat Jaya Tuah, Mitsuru Osaki
NEW PHYTOLOGIST, 174, 3, 516, 523, 2007
English, Scientific journal

Al-Fe interactions and growth enhancement in Melastoma malabathricum and Miscanthus sinensis dominating acid sulphate soils
Toshihiro Watanabe, Steven Jansen, Mitsuru Osaki
PLANT CELL AND ENVIRONMENT, 29, 12, 2124, 2132, Dec. 2006
English, Scientific journal

Internal mechanisms of plant adaptation to aluminum toxicity and phosphorus starvation in three tropical forages
Toshihiro Watanabe, Mitsuru Osaki, Hiromi Yano, Idupulapati M. Rao
JOURNAL OF PLANT NUTRITION, 29, 7, 1243, 1255, Jul. 2006
English, Scientific journal

Role of phosphoenolpyruvate carboxylase in the adaptation of a tropical forage grass to low-phosphorus acid soils
HH Begum, M Osaki, M Nanamori, T Watanabe, T Shinano, IM Rao
JOURNAL OF PLANT NUTRITION, 29, 1, 35, 57, Jan. 2006
English, Scientific journal

Different characteristics of nitrogen utilization between lupin and soybean: can lupin utilize organic nitrogen in soils?
T Watanabe, M Okamoto, S Misawa, M Urayama, M Osaki
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 84, 1, 20, 27, Jan. 2006
English, Scientific journal

Aluminum accumulation in the roots of Melastoma malabathricum, an aluminum-accumulating plant
T Watanabe, S Misawa, M Osaki
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 83, 11, 1518, 1522, Nov. 2005
English, Scientific journal

The beneficial effect of aluminium and the role of citrate in Al accumulation in Melastoma malabathricum
T Watanabe, S Jansen, M Osaki
NEW PHYTOLOGIST, 165, 3, 773, 780, Mar. 2005
English, Scientific journal

Interactive effects of Al, Ca and other cations on root elongation of rice cultivars under low pH
T Watanabe, K Okada
ANNALS OF BOTANY, 95, 2, 379, 385, Jan. 2005
English, Scientific journal

Efeito do aluminio no desenvolvimento e nutricao de Tapirira guianensis Aublet em solucao hidroponica
Ricardo Miranda de Britez, Carlos Bruno Reissmann, Toshihiro Watanabe, Mitsuru Osaki
Floresta, 32, 2, 171, 187, 2005

The function of a maize-derived phosphoenolpyruvate carboxylase (PEPC) in phosphorus-deficient transgenic rice
Hasna Hena Begum, Mitsuru Osaki, Takuro Shinano, Hiroaki Miyatake, Jun Wasaki, Takuya Yamamura, Toshihiro Watanabe
Soil Science and Plant Nutrition, 51, 4, 497, 506, 2005
English, Scientific journal

The distribution and phylogeny of aluminium accumulating plants in the ericales
S Jansen, T Watanabe, P Caris, K Geuten, F Lens, N Pyck, E Smets
PLANT BIOLOGY, 6, 4, 498, 505, Jul. 2004
English, Scientific journal

The evolution of aluminium accumulation in angiosperms
Steven Jansen, Toshihiro Watanabe, Steven Dessein, Elmar Robbrecht, Erik Smets
The Evolution of Plant Physiology, 467, 479, Elsevier Inc., 2004, [Peer-reviewed]
English, In book

Growth responses of cereal crops to organic nitrogen in the field
M Okamoto, K Okada, T Watanabe, N Ae
SOIL SCIENCE AND PLANT NUTRITION, 49, 3, 445, 452, Jun. 2003
English, Scientific journal

A comparative study of metal levels in leaves of some Al-accumulating Rubiaceae
S Jansen, T Watanabe, S Dessein, E Smets, E Robbrecht
ANNALS OF BOTANY, 91, 6, 657, 663, May 2003
English, Scientific journal

Breeding Wheat for Zinc Efficiency Improvement in Semi-arid Climate-A Review
Rishi Kumar BEHL, Mitsuru OSAKI, Jun WASAKI, Toshihiro WATANABE, Takuro SHINANO
Tropics, 12, 4, 295, 312, 日本熱帯生態学会, 2003
English

Nutritional characteristics of the leaves of native plants growing in adverse soils of humid tropical lowlands
M Osaki, T Watanabe, T Ishizawa, C Nilnond, T Nuyim, T Shinano, M Urayama, SJ Tuah
PLANT FOODS FOR HUMAN NUTRITION, 58, 2, 93, 115, 2003
English, Scientific journal

Mineral characteristics of the leaves of 166 plant species with different phylogeny in the temperate region
M Osaki, S Yamada, T Ishizawa, T Watanabe, T Shinano, SJ Tuah, M Urayama
PLANT FOODS FOR HUMAN NUTRITION, 58, 2, 139, 152, 2003
English, Scientific journal

Mineral characteristics of leaves of plants from different phylogeny grown in various soil types in the temperate region
M Osaki, S Yamada, T Ishizawa, T Watanabe, T Shinano, M Urayama
PLANT FOODS FOR HUMAN NUTRITION, 58, 2, 117, 137, 2003
English, Scientific journal

The relationship between aluminium and silicon accumulation in leaves of Faramea marginata (Rubiaceae)
RM Britez, T Watanabe, S Jansen, CB Reissmann, M Osaki
NEW PHYTOLOGIST, 156, 3, 437, 444, Dec. 2002
English, Scientific journal

Genotypic variability in phosphorus solubilizing activity of root exudates by pigeonpea grown in low-nutrient environments
S Ishikawa, JJ Adu-Gyamfi, T Nakamura, T Yoshihara, T Watanabe, T Wagatsuma
PLANT AND SOIL, 245, 1, 71, 81, Aug. 2002
English, Scientific journal

Role of organic acids in aluminum accumulation and plant growth in Melastoma malabathricum
T Watanabe, M Osaki
TREE PHYSIOLOGY, 22, 11, 785, 792, Aug. 2002
English, Scientific journal

Aluminium accumulation in leaves of 127 species in Melastomataceae, with comments on the order Myrtales
S Jansen, T Watanabe, E Smets
ANNALS OF BOTANY, 90, 1, 53, 64, Jul. 2002
English, Scientific journal

Mechanisms of adaptation to high aluminum condition in native plant species growing in acid soils: A review
T Watanabe, M Osaki
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS, 33, 7-8, 1247, 1260, 2002
English

Influence of aluminum and phosphorus on growth and xylem sap composition in Melastoma malabathricum L.
T Watanabe, M Osaki
PLANT AND SOIL, 237, 1, 63, 70, Nov. 2001
English, Scientific journal

Al uptake kinetics in roots of Melastoma malabathricum L. - an Al accumulator plant
T Watanabe, M Osaki, T Tadano
PLANT AND SOIL, 231, 2, 283, 291, Apr. 2001
English, Scientific journal

Comparison of aluminum tolerance and phosphate absorption between rape (Brassica napus L.) and tomato (Lycopersicum esculentum Mill.) in relation to organic acid exudation
HM Luo, T Watanabe, T Shinano, T Tadano
SOIL SCIENCE AND PLANT NUTRITION, 45, 4, 897, 907, Dec. 1999
English, Scientific journal

Effects of nitrogen source and aluminum on growth of tropical tree seedlings adapted to low pH soils
T Watanabe, M Osaki, T Tadano
SOIL SCIENCE AND PLANT NUTRITION, 44, 4, 655, 666, Dec. 1998
English, Scientific journal

Distribution and chemical speciation of aluminum in the Al accumulator plant, Melastoma malabathricum L.
T Watanabe, M Osaki, T Yoshihara, T Tadano
PLANT AND SOIL, 201, 2, 165, 173, Apr. 1998
English, Scientific journal

Nutritional characteristics in leaves of native plants grown in acid sulfate, peat, sandy podzolic, and saline soils distributed in Peninsular Thailand
M Osaki, T Watanabe, T Ishizawa, C Nilnond, T Nuyim, C Sittibush, T Tadano
PLANT AND SOIL, 201, 2, 175, 182, Apr. 1998, [Peer-reviewed]
English, Scientific journal

Nutritional characteristics of sage palm and oil palm in tropical peat soil
M Matsumoto, M Osaki, T Nuyim, A Jongskul, P Eam-on, Y Kitaya, M Urayama, T Watanabe, T Kawamukai, T Nakamura, C Nilnond, T Shinano, T Tadano
JOURNAL OF PLANT NUTRITION, 21, 9, 1819, 1841, 1998
English, Scientific journal

Aluminum-induced growth stimulation in relation to calcium, magnesium, and silicate nutrition in Melastoma malabathricum L.
T Watanabe, M Osaki, T Tadano
SOIL SCIENCE AND PLANT NUTRITION, 43, 4, 827, 837, Dec. 1997
English, Scientific journal

Beneficial effect of aluminum on growth of plants adapted to low pH soils
M Osaki, T Watanabe, T Tadano
SOIL SCIENCE AND PLANT NUTRITION, 43, 3, 551, 563, Sep. 1997
English, Scientific journal

イネおよびオオムギにおける耐塩性機構の差異
中村 卓司, 大崎 満, 安藤 理子, 但野 利秋
日本土壌肥料学雑誌, 67, 4, 459, 459, 一般社団法人 日本土壌肥料学会, 1996
Japanese

Titles of research presentation in Annual Meeting for Japanese Society of Soil Science and Plant Nutrition Hokkaido Branch (2024)
Gotou Eiji, Nakamura Takuji, Okada Kanako, Watanabe Toshihiro, Japanese Journal of Soil Science and Plant Nutrition, 96, 2, 189, 191, 05 Apr. 2025
Japanese Society of Soil Science and Plant Nutrition, Japanese

Dauciform root formation and nutrient status of Cyperaceae plants grown in harsh environments
和崎淳, 和崎淳, LI Xiao-Long, 山本晃弘, 青島大祐, 山田大綱, 井上竜輔, 大崎壮巳, 大崎壮巳, 中坪孝之, 中坪孝之, WANG Jinniu, PENG Fei, 渡部敏裕, ZHOU Jun, 日本植物生理学会年会(Web), 66th, 2025

Aluminum tolerance mechanisms of solfatara plants and involvement of endophytic fungi
青島大祐, 大崎壮巳, 大崎壮巳, 山本晃弘, 丸山隼人, 渡部敏裕, 中坪孝之, 中坪孝之, 和崎淳, 和崎淳, 日本植物生理学会年会(Web), 66th, 2025

Do soil conditions of serpentine and limestone areas limit plant growth?
井上竜輔, 山本晃弘, 渡部敏裕, 竹田一彦, 和崎淳, 中坪孝之, 日本生態学会大会講演要旨(Web), 72nd, 2025

Relationship between the spatial elemental distribution and the incidence of blossom-end rot in paprika.
荒川竜太, 村島和基, 渡部敏裕, 信濃卓郎, 丸山隼人, 園芸学研究 別冊, 24, 1, 2025

P4-4-3 トマトの尻腐れ果発症に対するグリシンベタイン葉面散布の影響（4-4 植物の代謝成分と農作物の品質 2024年度福岡大会）
渡邊 大起, 長谷川 公紀, 北岡 哲, 丸山 隼人, 信濃 卓郎, 金山 喜則, 中川 卓也, 佐々木 壮規, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 70, 79, 79, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

25 植物根が粘土鉱物中のセシウム動態に与える影響（北海道支部講演会）
水野 亜紗美, 圓尾 明子, 藤本 久恵, 黒川 耕平, 丸山 隼人, 中尾 淳, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 70, 241, 241, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P4-2-1 植物標本と蛍光 X 線分析を用いた野生植物の元素集積傾向の解析（6）新規マンガン超集積性植物（群）の発見とその元素集積傾向（4-2 植物の微量栄養素 2024年度福岡大会）
水野 隆文, 桑原 康輔, 安原 輝, 山下 純, 橋本 篤, 上野 大勢, 中村 進一, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 70, 60, 60, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P8-1-3 植物固有のセシウム吸収係数を用いた植物体放射性セシウム濃度予測の検討（第2報）（8-1 環境保全 2024年度福岡大会）
望月 杏樹, 久保 堅司, 藤村 恵人, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 70, 148, 148, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

24 Na によるシロバナルーピンの放射性セシウム吸収および体内分配の影響（北海道支部講演会）
岩井 純平, 菅 あやね, 丸山 隼人, 古川 純, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 70, 240, 240, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P3-2-1 根粒・菌根二重共生下におけるダイズのリン獲得の分子機構（3-2 土壌生物の応用と制御 2024年度福岡大会）
藤村 駿介, 馮 麟茜, 神山 拓也, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 70, 39, 39, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P4-3-9 コムギ子実中のミネラル成分変動に関わる遺伝的要因の探索（4-3 植物の有害元素 2024年度福岡大会）
市川 このか, 久保 堅司, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 70, 70, 70, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P4-3-12 アルミニウム集積植物メラストーマにおけるNrampファミリー遺伝子の機能解析（4-3 植物の有害元素 2024年度福岡大会）
新田 智美, 山口 夢乃, 丸山 隼人, 信濃 卓郎, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 70, 71, 71, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

4-3-10 根粒がマメ科植物における土壌から植物へのセシウム移行に与える影響（4-3 植物の有害元素 2024年度福岡大会）
村島 和基, 大熊 直生, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 70, 67, 67, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P4-3-11 強酸性硫気荒原に分布するミズスギとコシダの植物栄養学的特性（4-3 植物の有害元素 2024年度福岡大会）
渡部 敏裕, 今井 希, 平舘 俊太郎, 丸山 隼人, 和崎 淳, 日本土壌肥料学会講演要旨集, 70, 71, 71, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P4-3-7 テンサイのNaClによる生育促進について異なるナトリウム塩施与試験と遺伝子発現解析による考察（4-3 植物の有害元素 2024年度福岡大会）
野別 花, 熊谷 起一, 丸山 隼人, 信濃 卓郎, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 70, 69, 69, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

29 風化促進技術としての玄武岩の施与が土壌の化学性に与える影響（北海道支部講演会）
内林 大志, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 70, 242, 242, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P4-1-20 硫黄栄養状態がイネのリン欠乏応答に及ぼす影響（4-1 植物の多量栄養素 2024年度福岡大会）
山田 郁絵, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 70, 54, 54, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P7-1-7 ルーピン混植が主作物のリン濃縮スラグ肥料からの元素吸収に及ぼす影響（7-1 肥料および施肥法 2024年度福岡大会）
安孫子 実聖, 渡部 敏裕, 和崎 淳, 日本土壌肥料学会講演要旨集, 70, 128, 128, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

P7-2-10 異なる植物種における液肥GBの効果について（7-2 土壌改良資材 2024年度福岡大会）
佐々木 壮規, 中川 卓也, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 70, 143, 143, 26 Aug. 2024
一般社団法人 日本土壌肥料学会, Japanese

Titles of research presentation in Annual Meeting for Japanese Society of Soil Science and Plant Nutrition Hokkaido Branch (2023)
Okada Kanako, Nakamura Takuji, Onodera Masayuki, Watanabe Toshihiro, Japanese Journal of Soil Science and Plant Nutrition, 95, 3, 169, 170, 05 Jun. 2024
Japanese Society of Soil Science and Plant Nutrition, Japanese

Growth and ecophysiological responses of two species of poplar to two soil types and ozone levels
KOIKE Takayoshi, MASUI Noboru, WATANABE Yoko, WATANABE Toshihiro, Boreal Forest Research, 72, 15, 16, 29 Feb. 2024
Boreal Forest Society, Japanese

根粒がマメ科植物における土壌から植物へのセシウム移行に与える影響
村島和基, 大熊直生, 丸山隼人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 70, 2024

降下する放射性セシウムの農作物への付着・移行等の解明
八戸真弓, 山口紀子, 藤原英司, 井倉将人, 万福裕造, 浅枝諭史, 小椋智文, 平山孝, 信濃卓郎, 渡部敏裕, 丸山隼人, 農林水産省関係放射能調査研究年報, 2022, 2024

降下する放射性セシウムの農作物への付着・移行等の解明
八戸真弓, 山口紀子, 藤原英司, 井倉将人, 万福裕造, 浅枝諭史, 小椋智文, 平山孝, 信濃卓郎, 渡部敏裕, 丸山隼人, 農林水産省関係放射能調査研究年報, 2023, 2024

Plants grow in ultrabasic soil and highly acidic volcanic soil: Ion profiles of plants distributed in two contrasting soils
山本晃弘, 井上竜輔, 渡部敏裕, 中坪孝之, 和崎淳, 日本生態学会大会講演要旨(Web), 71st, 2024

根粒・菌根二重共生下におけるダイズのリン獲得の分子機構
藤村駿介, 馮麟茜, 神山拓也, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 70, 2024

畑地への玄武岩施与が土壌化学性およびダイズの生育に及ぼす影響と風化促進の可能性
内林大志, 丸山隼人, 渡部敏裕, 当真要, 濱本昌一郎, 楊椅麟, 朱顔, 小坂弦, 中尾淳, 黒川耕平, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 70, 2024

Effect of bone charcoal and ectomycorrhizal fungi on the growth of larch seedlings. -Expectations of trees as grave-marker tree for arboricultural burial-
KOIKE, Takayoshi, MASUI, Noboru, TAMAI, Yutaka, SASAKI, Keiko, FIJITO, Eishi, ITO, Yuya, WATANABE, Toshihiro, UEDA, Hirofumi, Technical report for boreal forest conservation of the Hokkaido University Forests, 42, 38, 43, 2024
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

Use of cover crops on fallow land for suppressing weed in experimental nursery fields
KOIKE, Takayoshi, MASUI, Noboru, KITAOKA, Satoshi, SASAKI, Keiko, FUJITO, Eishi, HIRATA, Toshiyuki, WATANABE, Toshihiro, Technical report for boreal forest conservation of the Hokkaido University Forests, 42, 34, 37, 2024
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

Plant-insect association analysis using an open system ozone addition facility. -The case study on alder leaf beetle and white birch-
MASUI, Noboru, KOIKE, Takayoshi, MATSUURA, Hideyuki, SASAKI, Keiko, FIJITO, Eishi, WATANABE, Toshihiro, Technical report for boreal forest conservation of the Hokkaido University Forests, 42, 44, 49, 2024
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

Relationship between elements and metabolites in paprika pericarp under different nitrogen supply.
村島和基, 荒川竜太, 丸山隼人, 渡部敏裕, 信濃卓郎, 園芸学研究 別冊, 23, 2, 2024

P4-4-4 マルチオミクスによるダイズ子実収量に関わる複合的要因解析（4-4 植物の代謝成分と農作物の品質 2023年度愛媛大会）
村島 和基, 丸山 隼人, 渡部 敏裕, 大津 厳生, 草野 都, 杉山 暁史, 小林 奈通子, 濱本 昌一郎, 福島 敦史, 大熊 直生, 藤原 風輝, 二瓶 直登, 市橋 泰範, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 69, 77, 77, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

P8-1-1 植物固有のセシウム吸収係数を用いた植物体放射性セシウム濃度予測の検討（8-1 環境保全 2023年度愛媛大会）
望月 杏樹, 鈴木 政祟, 久保 堅司, 藤村 恵人, 浅枝 諭史, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 69, 139, 139, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

P4-1-4 コムギ品種間におけるリン獲得と根圏細菌叢の関係性（4-1 植物の多量栄養素 2023年度愛媛大会）
今井 智葉, 青山 奈央, 能勢 結衣, 熊石 妃恵, 市橋 泰範, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 69, 53, 53, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

4-1-15 植物標本と蛍光X線分析を用いた野生植物の元素集積傾向の解析（5）土壌の違いが野生植物の各種元素の集積量と相関性に与える影響（4-1 植物の多量栄養素 2023年度愛媛大会）
水野 隆文, 桑原 康輔, 近藤 大地, 伊東 拓朗, 村井 良徳, 山下 純, 橋本 篤, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 69, 48, 48, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

4-1-24 ミナトカモジグサ−菌根菌共生系におけるリンの吸収・可給化機構の根局所的評価（4-1 植物の多量栄養素 2023年度愛媛大会）
永山 航平, 熊石 紀恵, 市橋 泰範, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 69, 51, 51, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

P4-3-2 アルミニウム集積植物メラストーマ（Melastoma malabathricum）のアルミニウムによる鉄毒性緩和機構に関する研究（4-3 植物の有害元素 2023年度愛媛大会）
山口 夢乃, 丸山 隼人, 山下 寛人, 一家 崇志, 信濃 卓郎, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 69, 69, 69, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

16 異なる土壌養分条件における土壌からのCs移行に対するダイズの根粒着生の影響（北海道支部講演会）
村島 和基, 外島 海斗, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 69, 218, 218, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

P8-1-3 異なる粒子径の玄武岩資材がダイズの元素吸収に与える影響（8-1 環境保全 2023年度愛媛大会）
内林 大志, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 69, 140, 140, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

17 中長期的な有機肥料施与による作物の放射性セシウム吸収抑制の可能性（北海道支部講演会）
鈴木 政崇, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 69, 218, 218, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

P4-1-6 イネにおける硫黄とリンの吸収・体内分配のクロストーク（4-1 植物の多量栄養素 2023年度愛媛大会）
山田 郁絵, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 69, 54, 54, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

4-1-17 宮島に自生するクラスター根形成種ヤマモモの葉身元素組成と雌雄間差（4-1 植物の多量栄養素 2023年度愛媛大会）
和崎 淳, 愛原 健司, 渡部 敏裕, 坪田 博美, 日本土壌肥料学会講演要旨集, 69, 49, 49, 04 Sep. 2023
一般社団法人 日本土壌肥料学会, Japanese

異なる土壌養分条件における土壌からのCs移行に対するダイズの根粒着生の影響
村島和基, 外島海斗, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 69, 2023

植物固有のセシウム吸収係数を用いた植物体放射性セシウム濃度予測の検討
望月杏樹, 鈴木政祟, 久保堅司, 藤村恵人, 浅枝諭史, 浅枝諭史, 丸山隼人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 69, 2023

Variation in fatty acid composition in leaves of birch (Betula platyphylla var. japonica) in a free-air ozone fumigation system : A plant-insect communication perspective
Masui, Noboru, Inoue, Shiro, Matsuura, Hideyuki, Evgenios, Agatokleous, Fujito, Eishi, Watanabe, Toshihiro, Koike, Takayoshi, Technical report for boreal forest conservation of the Hokkaido University Forests, 41, 21, 29, 2023
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

Effects of bone charcoal and ectomycorrhizal fungi on the growth of Japanese larch seedlings : German natural burial initiative to return burnt bones to the earth at the roots of forests
Koike, Takayoshi, Ueda, Hirofumi, Koda, Kei'ichi, Kitaoka, Satoshi, Sasaki, Keiko, Fujito, Eishi, Ito, Yuya, Takai, Yutaka, Watanabe, Toshihiro, Technical report for boreal forest conservation of the Hokkaido University Forests, 41, 16, 20, 2023
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

マルチオミクスによるダイズ子実収量に関わる複合的要因解析
村島和基, 丸山隼人, 渡部敏裕, 大津厳生, 草野都, 杉山暁史, 小林奈通子, 濱本昌一郎, 福島敦史, 大熊直生, 藤原風輝, 藤原風輝, 二瓶直登, 市橋泰範, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 69, 2023

Effect of nitrogen nutrient conditions on elemental composition and functional constituents in paprika pericarps.
荒川竜太, 村島和基, 丸山隼人, 渡部敏裕, 信濃卓郎, 園芸学研究 別冊, 22, 1, 2023

5 異なる交換性カリレベルがダイズのカリウム・セシウムの体内分配に与える影響（北海道支部講演会 2021年度支部講演会）
鈴木 政崇, 藤本 久恵, 浅枝 諭史, 丸山 隼人, 久保 堅司, 藤村 恵人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 68, 174, 174, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

4-1-8 植物標本と蛍光X線分析を用いた野生植物の元素集積傾向の解析（4）日本の野生植物におけるイオウとリンの集積傾向とその正の相関（4-1 植物の多量栄養素 2022年度東京大会）
水野 隆文, 近藤 大地, 河西 皓仁, 伊東 拓朗, 村井 良徳, 山下 純, 橋本 篤, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 68, 38, 38, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

P3-2-9 ミナトカモジグサ菌根共生系におけるリン獲得の菌種特徴的分子機構（3-2 土壌生物の応用と制御 2022年度東京大会）
永山 航平, 菅井 徹人, 佐藤 匠, 神山 拓也, 市橋 泰範, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 68, 35, 35, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

4-3-8 硫気荒原に生息するツクシテンツキのアルミニウム耐性における硫黄の役割（4-3 植物の有害元素 2022年度東京大会）
外島 海斗, 丸山 隼人, 和崎 淳, 平舘 俊太郎, 神山 拓也, 信濃 卓郎, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 68, 55, 55, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

P4-3-10 異なる窒素条件下において塩化ナトリウムがテンサイの生育に与える影響（4-3 植物の有害元素 2022年度東京大会）
熊谷 起一, 北岡 哲, 小濱 諒太, 丸山 隼人, 信濃 卓郎, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 68, 59, 59, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

9 葉中の窒素分配からみたニセアカシアの光利用特性（北海道支部講演会 2021年度支部講演会）
北岡 哲, 渡邊 陽子, 信濃 卓郎, 渡部 敏裕, 佐藤 冬樹, 小池 孝良, 日本土壌肥料学会講演要旨集, 68, 175, 175, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

4-1-1 ダイズ根粒・菌根共生系における土壌リン獲得の制御機構（4-1 植物の多量栄養素 2022年度東京大会）
丸山 隼人, 浅尾 真結子, 神山 拓也, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 68, 36, 36, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

P4-3-2 シロバナルーピンのセシウム吸収・分配における元素間相互作用（4-3 植物の有害元素 2022年度東京大会）
菅 あやね, 丸山 隼人, 古川 純, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 68, 57, 57, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

10 オゾン増加環境でのブナとミズナラ稚樹の光合成と養分特性 開放系オゾン付加施設での事例（北海道支部講演会 2021年度支部講演会）
小池 孝良, 北岡 哲, 増井 昇, 渡部 敏裕, 佐藤 冬樹, 日本土壌肥料学会講演要旨集, 68, 176, 176, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

4-2-14 ダイズのCs体内分配に関わるミネラルネットワークの経時的変動（4-2 植物の微量栄養素 2022年度東京大会）
村島 和基, 丸山 隼人, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 68, 49, 49, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

P4-3-7 アルミニウム集積植物メラストーマ （Melastoma malabathricum）の好アルミニウム性における鉄の関与（4-3 植物の有害元素 2022年度東京大会）
山口 夢乃, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 68, 58, 58, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

P4-1-10 異なる光質が葉野菜の体内元素濃度に及ぼす影響（4-1 植物の多量栄養素 2022年度東京大会）
芹澤 和樹, 大橋(兼子) 敬子, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 68, 44, 44, 05 Sep. 2022
一般社団法人 日本土壌肥料学会, Japanese

Growth and out-break of insects and disease of 2 species of poplar sprouts grown under elevated Ozone
KOIKE TAKAYOSHI, Masui Noboru, Kitaoka Satoshi, Watanabe Yoko, Satoh Fuyuki, Watanabe Toshihiro, The Japanese Forest Society Congress, 133, 21, 30 May 2022
[in Japanese], The Japanese Forest Society, English

Outbreak of insects and diseases of two poplar cuttings grown under free air elevated ozone
Masui Noboru, Koike Takayoshi, Kitaoka Satoshi, Watanabe Yoko, Satoh Fuyuki, Watanabe Toshihiro, Tree and Forest Health, 26, 1, 26, 27, 31 Jan. 2022
Tree Health Research Society, Japan, Japanese

ダイズのCs体内分配に関わるミネラルネットワークの経時的変動
村島和基, 丸山隼人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 68, 2022

異なる交換性カリレベルがダイズのカリウム・セシウムの体内分配に与える影響
鈴木政崇, 藤本久恵, 浅枝諭史, 浅枝諭史, 丸山隼人, 久保堅司, 藤村恵人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 68, 2022

ダイズ根粒・菌根共生系における土壌リン獲得の制御機構
丸山隼人, 浅尾真結子, 神山拓也, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 68, 2022

硫気荒原に生息するツクシテンツキのアルミニウム耐性における硫黄の役割
外島海斗, 丸山隼人, 和崎淳, 平舘俊太郎, 神山拓也, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 68, 2022

ミナトカモジグサ菌根共生系におけるリン獲得の菌種特徴的分子機構
永山航平, 菅井徹人, 菅井徹人, 佐藤匠, 神山拓也, 市橋泰範, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 68, 2022

Seasonal change in outbreak of disease, insect grazing and growth in two species of Poplar cuttings raised under a free-air Ozone fumigation : a case of 2021
Masui, Noboru, Kitaoka, Satoshi, Watanabe, Yoko, Watanabe, Toshihiro, Fujito, Eishi, Sasaki, Keiko, Satoh, Fuyuki, Agathokleous, Evgenios, Koike, Takayoshi, Technical report for boreal forest conservation of the Hokkaido University Forests, 40, 17, 24, 2022
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

Al耐性遺伝子PGIP1の発現量ゲノムワイド関連解析によるPGIP1発現を制御するシグナル伝達経路と遺伝子の同定
AGRAHARI Raj Kishan, 榎本拓央, 伊藤弘樹, 中野友貴, 柳瀬笑子, 渡部敏裕, 井内聖, 小林正智, 山本義治, 小山博之, 小林佑理子, 日本土壌肥料学会講演要旨集(Web), 68, 2022

Forests and Forestry in the Republic of Lithuania.: A report of the JSPS Joint Research
Kitaoka Satoshi, Watanabe Toshihiro, Koike Takayoshi, Japanese Journal of International Forest and Forestry, 112, 15, 31 Dec. 2021
Japan International Forestry Promotion and Cooperation Center, Japanese

リトアニアの森林と林業—Forests and Forestry in the Republic of Lithuania
北岡 哲, 渡部 敏裕, 小池 孝良, 海外の森林と林業 = Japanese journal of international forest and forestry / 海外の森林と林業編集委員会 編, 112, 15, 19, Dec. 2021
東京 : 国際緑化推進センター, Japanese

Measurement of biogenic volatile organic compounds (BVOCs）and dynamics of insect herbivories
増井, 昇, 谷, 晃, 松浦, 英幸, 渡部, 敏裕, 藤戸, 永志, 佐々木, 圭子, 佐藤, 冬樹, 高木, 健太郎, アガトクレオス, エフゲニオス, 小池, 孝良, Technical report for boreal forest conservation of the Hokkaido University Forests, 39, 1, 5, Nov. 2021
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

P4-2-6 ハンドヘルド型蛍光X 線分析計を用いた野生植物の元素集積解析（3）野生植物の硫黄（S）含有量に関する調査・解析（4-2 植物の微量栄養素 2021年度北海道大会）
近藤 大地, 河西 皓仁, 渡部 敏裕, 橋本 篤, 水野 隆文, 日本土壌肥料学会講演要旨集, 67, 67, 67, 03 Sep. 2021
一般社団法人 日本土壌肥料学会, Japanese

4-2-20 ハンドヘルド型蛍光X 線分析計を用いた野生植物の元素集積解析（2）石灰岩土壌植物の元素集積特性（4-2 植物の微量栄養素 2021年度北海道大会）
水野 隆文, 河西 皓仁, 近藤 大地, 渡部 敏裕, 橋本 篤, 日本土壌肥料学会講演要旨集, 67, 65, 65, 03 Sep. 2021
一般社団法人 日本土壌肥料学会, Japanese

Biological stress on two poplar species grown under a free air ozone exposure system
Koike Takayoshi, Masui Noboru, Watanabe Yoko, Sugai Tetsuto, Satoh Fuyuki, Watanabe Toshihiro, The Japanese Forest Society Congress, 132, 100, 24 May 2021
[in Japanese], The Japanese Forest Society, Japanese

シロバナルーピン根域における土壌中リン動態に与える影響の局所的評価
菅あやね, 丸山隼人, 和崎淳, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 67, 2021

ミナトカモジグサの生育および養分状態に応じたリン獲得の分子制御機構
永山航平, 菅井徹人, 菅井徹人, 佐藤匠, 熊石紀恵, 市橋泰範, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 67, 2021

トマト尻腐れ発症率の品種間差と果実への無機元素蓄積の関係
長谷川公紀, 渡邊涼太郎, 丸山隼人, 金山喜則, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 67, 2021

コムギコアコレクションを用いた可塑的土壌リン獲得形質の品種間差
青山奈央, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 67, 2021

異なる土壌における白花ルーピンの根分泌物分布特性の可視化
尹永根, 鈴井伸郎, 三好悠太, 海野佑介, 丸山隼人, 和崎淳, 菅あやね, 圓尾明子, 渡部敏裕, 橋本洋平, 内田翔子, 信濃卓郎, 河地有木, 日本土壌肥料学会講演要旨集(Web), 67, 2021

異なる圃場におけるダイズへの放射性セシウムの移行比較
鈴木政崇, 藤本久恵, 浅枝諭史, 浅枝諭史, 丸山隼人, 久保堅司, 藤村恵人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 67, 2021

異なる窒素条件下におけるパプリカ果実の元素組成と尻腐れ果発症との関係
荒川竜太, 村島和基, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 67, 2021

根圏の有機物・ガスに関する植物栄養学研究の一視点-根の細胞壁・細胞膜での各種の化学的・物理的な力と下流遺伝子群への注目
我妻忠雄, 俵谷圭太郎, 程為国, 渡部敏裕, 丸山隼人, 和崎淳, 日本土壌肥料学会講演要旨集(Web), 67, 2021

温度処理にともなう元素吸収・分配がパプリカの尻腐れ果発症に与える影響
鳥山星呂, 荒川竜太, 村島和基, 丸山隼人, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 67, 2021

シロバナルーピンの放射性セシウム吸収特性と根圏土壌中元素動態の関係
浅枝諭史, 浅枝諭史, 丸山隼人, 藤本久恵, 久保堅司, 藤村恵人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 67, 2021

異なる窒素条件下におけるパプリカ果実の機能性成分解析
村島和基, 荒川竜太, 丸山隼人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 67, 2021

火山性強酸性土壌に生育する植物の葉身元素組成の特徴
和崎淳, 和崎淳, 山本晃弘, 山本晃弘, 丸山隼人, 中坪孝之, 中坪孝之, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 67, 2021

高オゾン環境下でのヤマナラシ属2種の成長と病虫害の観察-中間報告-
増井昇, 菅井徹人, 渡邊陽子, 渡部敏裕, 塩尻かおり, 佐々木圭子, 藤戸永司, 荒川圭太, 佐藤冬樹, 小池孝良, 北方森林保全技術(Web), 38, 2021

土壌の締め固めと苗木の生育 : 根系への注目
菅井 徹人, 小池 孝良, 渡部 敏裕, Shinrin Kagaku, 90, 14, 18, 01 Oct. 2020
The Japanese Forest Society, Japanese

4-2-16 H₂O₂ 高放出性植物の鉄存在下での根圏フェントン反応によるメタン、農薬類の分解（4-2 植物の微量栄養素 2020年度岡山大会）
我妻 忠雄, 大谷 卓, 清家 伸康, 並木 小百合, 俵谷 圭太郎, 渡部 敏裕, 田中 和博, 飯野 由一郎, 伊藤 太地, 佐藤 毅, 冨田 慎虎, 絵面 智宏, 日本土壌肥料学会講演要旨集, 66, 57, 57, 01 Sep. 2020
一般社団法人 日本土壌肥料学会, Japanese

20 HMG 遺伝子を過剰発現させたイネのCd イオンストレス耐性（北海道支部講演会，2019年度各支部会）
漆原 まりあ, 亀岡 悠香, 丸山 隼人, 我妻 忠雄, 黒田 善治, 前島 恵理子, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 66, 199, 199, 01 Sep. 2020
一般社団法人 日本土壌肥料学会, Japanese

4-2-22 ハンドヘルド型蛍光X 線分析計を用いた野生植物の元素集積解析（1）データ収集法の検討（4-2 植物の微量栄養素 2020年度岡山大会）
水野 隆文, 佐藤 奏美, 岡野 隆志, 渡部 敏裕, 橋本 篤, 亀岡 孝治, 日本土壌肥料学会講演要旨集, 66, 59, 59, 01 Sep. 2020
一般社団法人 日本土壌肥料学会, Japanese

重機導入に伴う踏圧の影響評価の動向 : 中欧を中心とした研究事例—Trends in the evaluation of the impact of pedaling pressure due to the introduction of heavy machinery : Case studies focusing on Central Europe
小池 孝良, 菅井 徹人, 渡部 敏裕, 佐藤 冬樹, 海外の森林と林業 = Japanese journal of international forest and forestry / 海外の森林と林業編集委員会 編, 108, 27, 30, Aug. 2020
東京 : 国際緑化推進センター, Japanese

Responses of root systems in hybrid larch F₁ seedlings grown under compacted soil
Sugai Tetsuto, Tamai Yutaka, Koike Takayoshi, Sato Fuyuki, Watanabe Toshihiro, The Japanese Forest Society Congress, 131, 339, 25 May 2020
[in Japanese], The Japanese Forest Society, Japanese

Growth of Sakhalin fir and environmental changes in Sapporo and its vicinity
Koike Takayoshi, Moser-Reischl Astrid, Qu Laiye, Watanabe Toshihiro, Ulbricht Matthias, Roetzer Thomas, The Japanese Forest Society Congress, 131, 263, 25 May 2020
[in Japanese], The Japanese Forest Society, Japanese

A large scale evaluation of effect of changing environment on forest stands: results from a Free Air CO2 or O3 enrichment system　　　　　　　　　　　　　　　
Koike T, Eguchi N, Watanabe T, Ichikawa K, Fujito E・Qu LY, Watanabe M, Watanabe Y, Agathokleou E, Kitao M, Takagi K, Hiura T, Nakaba S, Satomura T, Funada R, Satoh F, 北方森林保全技術, 37, Feb. 2020
Japanese, Introduction research institution

Urban ecology under Ozone pollution: an experimental approach with a Free-Air Controlled Elevated (FACE) O3 system supported by the JST (Japan Science and Technology Agency)　　　　　　　　　　　　　　　
KOIKE Takayoshi, 北方森林保全技術, 37, Feb. 2020
Japanese, Introduction research institution

コムギコアコレクションを用いた根圏リンの形態別評価とリン獲得形質との関係
青山奈央, 森田洸介, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 66, 2020

アルミニウム集積植物Melastoma marabathricumにおけるMATE遺伝子の機能解析
坂口文香, 吉井健祐, 丸山隼人, 佐々木孝行, 西田翔, 和崎淳, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 66, 2020

ナトリウムによるテンサイの生育促進と光環境の関係
小濱諒太, 合田健登, 合田健登, 丸山隼人, 菅井徹人, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 66, 2020

火山性強酸性土壌に生育するツクシテンツキのイオノミクス解析
藤原久貴, 丸山隼人, 和崎淳, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 66, 2020

白花ルーピンのK欠乏下における根圏土壌中K及びCsの形態変化
藤本久恵, 高雄惇英, 丸山隼人, 俵谷圭太郎, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 66, 2020

土壌窒素バランスに対する小麦の葉位別イオノーム解析と光合成機能の関連
菅井徹人, 丸山隼人, 渡部敏裕, 信濃卓郎, 日本土壌肥料学会講演要旨集(Web), 66, 2020

ナトリウムによるテンサイの生育促進機構に関する研究
小濱諒太, 合田健登, 合田健登, 丸山隼人, 菅井徹人, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 66, 2020

ハーブの養分欠乏ストレスと機能性成分とのかかわり
伊東和輝, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 66, 2020

土壌リン・窒素制限下におけるダイズの生育とリン獲得経路の経時的評価
浅尾真結子, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 66, 2020

P4-1-17 道央・十勝地域の大豆展開葉元素含有率とその生産性の関係（4-1 植物の多量栄養素，2019年静岡大会）
中村 卓司, 渡部 敏裕, 長南 友也, 林 怜史, 辻 博之, 岡 紀邦, 森本 晶, 大友 量, 村上 則幸, 日本土壌肥料学会講演要旨集, 65, 53, 53, 03 Sep. 2019
一般社団法人 日本土壌肥料学会, Japanese

8-1-16 高オゾン環境下で生産された落葉広葉樹の葉リターの分解過程（8-1 環境保全，2019年静岡大会）
野中 佳祐, 石 聡, 李 瑞利, 菅井 徹人, 佐藤 冬樹, 渡部 敏裕, 小池 孝良, 日本土壌肥料学会講演要旨集, 65, 132, 132, 03 Sep. 2019
一般社団法人 日本土壌肥料学会, Japanese

Growth and Physiological responses of hybrid larch F₁ seedling to soil compaction and nitrogen loading
Sugai Tetsuto, Yokoyama Satoko, Fan Rong, Sato Fuyuki, Watanabe Toshihiro, Koike Takayoshi, The Japanese Forest Society Congress, 130, 767, 27 May 2019
[in Japanese], The Japanese Forest Society, Japanese

土場を想定した踏圧が植栽苗木の成長に及ぼす影響調査(予報) : グイマツ雑種F₁を対象にした試験地の設定
菅井 徹人, 横山 聡子, 市川 一, 藤戸 永志, 藤田 早紀, 玉井 裕, 渡部 敏裕, 佐藤 冬樹, 小池 孝良, 北方森林保全技術, 36, 20, 24, Feb. 2019
北海道大学北方生物圏フィールド科学センター森林圏ステーション, Japanese

硫気荒原に分布する耐酸性植物種のイオンプロファイル
和崎淳, 山本晃弘, 齋藤天翔, 坪田博美, 渡部敏裕, 中坪孝之, 日本植物生理学会年会(Web), 60th, 2019

産地別トドマツ苗木の遮光処理に対する生理成長応答
菅井徹人, 石塚航, 丸山隼人, 渡部敏裕, 日本生態学会大会講演要旨(Web), 66th, 2019

HMG遺伝子を過剰発現させたイネの金属イオンストレス耐性
亀岡悠香, 亀岡悠香, 丸山隼人, 我妻忠雄, 黒田昌治, 漆原まりあ, 前島恵理子, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 65, 2019

異なる光条件下におけるナトリウムの西洋フダンソウに対する影響
合田健登, 菅井徹人, 丸山隼人, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 65, 2019

窒素欠乏下におけるコムギの光合成特性とモリブデン集積の関係
菅井徹人, 徳長そよ香, 丸山隼人, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 65, 2019

コムギにおける低リン耐性および土壌蓄積リン利用能の品種間差
森田洸介, 渡部敏裕, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 65, 2019

HMG遺伝子を過剰発現させたイネのアルミニウム耐性獲得について
亀岡悠香, 丸山隼人, 我妻忠雄, 黒田昌治, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 65, 2019

白花ルーピンのカリウム欠乏下における不可給態カリウム及びセシウムの可給化機構
藤本久恵, 高雄惇英, 丸山隼人, 俵谷圭太郎, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 65, 2019

養分欠乏がダイズ種子に与える影響の品種間比較
浅尾真結子, 丸山隼人, 信濃卓郎, 渡部敏裕, 日本土壌肥料学会講演要旨集(Web), 65, 2019

日本のコムギコアコレクションにおける土壌中難利用性リン可給化能の品種間比較
青山奈央, 森田洸介, 渡部敏裕, 信濃卓郎, 丸山隼人, 日本土壌肥料学会講演要旨集(Web), 65, 2019

異なる窒素栄養および温度条件下で栽培したパプリカ果実の元素動態に関する網羅的解析
荒川竜太, 藤本久恵, 亀岡悠香, 鳥山星呂, 渡部敏裕, 丸山隼人, 園芸学研究 別冊, 18, 2, 2019

13 異なる光条件下におけるナトリウムの西洋フダンソウに対する影響（北海道支部講演会）
合田 健登, 菅井 徹人, 丸山 隼人, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 65, 0, 227, 227, 2019
一般社団法人 日本土壌肥料学会, Japanese

40 窒素欠乏下におけるコムギの光合成特性とモリブデン集積の関係（北海道支部講演会）
菅井 徹人, 徳長 そよ香, 丸山 隼人, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 65, 0, 236, 236, 2019
一般社団法人 日本土壌肥料学会, Japanese

42 HMG遺伝子を過剰発現させたイネのアルミニウム耐性獲得について（北海道支部講演会）
亀岡 悠香, 丸山 隼人, 我妻 忠雄, 黒田 昌治, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 65, 0, 236, 236, 2019
一般社団法人 日本土壌肥料学会, Japanese

41 コムギにおける低リン耐性および土壌蓄積リン利用能の品種間差（北海道支部講演会）
森田 洸介, 渡部 敏裕, 丸山 隼人, 日本土壌肥料学会講演要旨集, 65, 0, 236, 236, 2019
一般社団法人 日本土壌肥料学会, Japanese

P4-1-16 養分欠乏がダイズ種子に与える影響の品種間比較（4-1 植物の多量栄養素，2019年静岡大会）
浅尾 真結子, 丸山 隼人, 信濃 卓郎, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 65, 0, 53, 53, 2019
一般社団法人 日本土壌肥料学会, Japanese

P4-1-7 日本のコムギコアコレクションにおける土壌中難利用性リン可給化能の品種間比較（4-1 植物の多量栄養素，2019年静岡大会）
青山 奈央, 森田 洸介, 渡部 敏裕, 信濃 卓郎, 丸山 隼人, 日本土壌肥料学会講演要旨集, 65, 0, 50, 50, 2019
一般社団法人 日本土壌肥料学会, Japanese

P4-1-9 白花ルーピンのカリウム欠乏下における不可給態カリウム及びセシウムの可給化機構（4-1 植物の多量栄養素，2019年静岡大会）
藤本 久恵, 高雄 惇英, 丸山 隼人, 俵谷 圭太郎, 信濃 卓郎, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 65, 0, 50, 50, 2019
一般社団法人 日本土壌肥料学会, Japanese

P4-3-7 HMG遺伝子を過剰発現させたイネの金属イオンストレス耐性（4-3 植物の有害元素，2019年静岡大会）
亀岡 悠香, 丸山 隼人, 我妻 忠雄, 黒田 昌治, 漆原 まりあ, 前島 恵理子, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 65, 0, 69, 69, 2019
一般社団法人 日本土壌肥料学会, Japanese

土壌踏圧と森林の科学 − 欧州と札幌の事例から −　　　　　　　　　　　　　　　
菅井徹人, 渡部敏裕, 佐藤冬樹, 小池 孝良, 森林技術, 918:, 30, 33, Sep. 2018, [Peer-reviewed]
Japanese, Introduction other

P8-1-12 Effects of salinity stress on the growth and physiology of hybrid larch F₁ and its parents exposed to elevated ozone（ポスター，8-1 環境保全，2018年度神奈川大会）
菅井 徹人, 汪 雁楠, 張 超群, 来田 和人, 小池 孝良, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 64, 146, 146, 29 Aug. 2018
一般社団法人 日本土壌肥料学会, Japanese

4-1-4 日本に在来するカヤツリグサ科植物のダウシフォーム根形成と低リン耐性（4-1 植物の多量栄養素，2018年度神奈川大会）
和崎 淳, 松山 理絵, 枡田 元気, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 64, 46, 46, 29 Aug. 2018
一般社団法人 日本土壌肥料学会, Japanese

26 ダイズにおける開花期展開葉の元素含有率と収量の関係（北海道支部講演会，2017年度各支部会）
渡部 敏裕, 長南 友也, 林 怜史, 中村 卓司, 日本土壌肥料学会講演要旨集, 64, 243, 243, 29 Aug. 2018
一般社団法人 日本土壌肥料学会, Japanese

トマト染色体置換系統における果実の細胞壁構成と尻腐れ発症の関係性
渡邊涼太郎, 富崎亮太, 丸山隼人, 金山喜則, 渡部敏裕, 日本土壌肥料学会講演要旨集, 64, 50, 29 Aug. 2018
Japanese

Fluctuation effect of potassium and phosphoric acid fertilization and planting density on the yield and quality of beet and the relationship between the degrees of fluctuation on each field and soil analysis values
Takada Shuichi, Watanabe Toshihiro, Osaki Mitsuru, Japanese Journal of Soil Science and Plant Nutrition, 89, 2, 95, 107, 05 Apr. 2018, [Peer-reviewed]
Potassium and phosphoric acid fertilization and planting density analyses were conducted across 31 fields in Tokachi District, Hokkaido, in 1990. The three test plots were arranged side-by-side in the same field, each with an area of 15 m² and designed in a fully randomized method, with no repetition. Seven levels of potassium fertilization [range, 0–249 kg-K ha⁻¹ (0–300 kg-K₂O ha⁻¹)], six levels of phosphoric acid fertilization [0–273 kg-P ha⁻¹ (0–625 kg-P₂O₅ ha⁻¹)], and planting density [50,000–90,000 plants ha⁻¹] were examined. To quantify the effect of fertilization and planting density on the yield and quality of sugar beet, these factors were varied, and linear regression coefficients were calculated for each studied field. Next, the correlation between these regression coefficients and soil analysis values was examined. Some significant correlation was found between these variables, and these signs of significant correlations were opposite between potassium and phosphoric acid fertilizations. A negative significant correlation was found between regression coefficients of potassium and phosphoric acid fertilization effects on the root weight and sugar yield.

The effect of potassium and phosphoric acid fertilization can be classified into the following three groups: group A, strong effect of potassium; group B, strong effect of phosphoric acid; and group C, strong effect of both potassium and phosphoric acid. Hierarchical cluster analysis was performed for the regression coefficients of potassium and phosphoric acid fertilization effects on the sugar yield. Therefore, we investigated a method to classify cultivation fields into the abovementioned groups using canonical discriminant analysis for soil parameters and developed a prototype for automatic calculation using Microsoft Excel.

This model is a field discrimination model derived from the field test of sugar beet, but it seems to be helpful in estimating the potassium and phosphate acidic characteristics of the field including other crops., Japanese Society of Soil Science and Plant Nutrition, Japanese

窒素付加とオゾン暴露がカラマツ属2種苗木の針葉成分に与える影響
菅井徹人, 渡部敏裕, 北尾光俊, 来田和人, 小池孝良, 日本森林学会大会学術講演集, 129th, 96, 26 Mar. 2018
Japanese

トドマツ当年葉のクロロフィル蛍光反応に対するオゾン暴露の影響
菅井徹人, 北尾光俊, 渡部敏裕, 小池孝良, 北方森林研究, 66, 41‐42, 28 Feb. 2018
Japanese

塩類化土壌で生育させたグイマツ苗木初期成長に対するオゾン付加の影響
WANG Yannan, 菅井徹人, 坪奈津美, 渡部敏裕, 小池孝良, 北方森林研究, 66, 51‐54, 28 Feb. 2018
Japanese

塩類化土壌とオゾン暴露に対するカラマツ属3種苗木の生理応答~クロロフィル蛍光応答に着目して~
坪奈津美, WANG Yannan, 菅井徹人, 渡部敏裕, 斎藤秀之, 澁谷正人, 小池孝良, 北方森林研究, 66, 55‐57, 28 Feb. 2018
Japanese

シロイヌナズナ野生系統を用いたカドミウム耐性のゲノムワイド関連解析
中野友貴, 楠和隆, TAYLOR Gregory J., 渡部敏裕, 井内聖, 小林正智, 小山博之, 小山博之, 小林佑理子, 小林佑理子, 日本植物生理学会年会(Web), 59th, 2018

Effect of and foliar spraying of molybdenum (Mo) in flowering stage and the seed Mo enrichment on productivity of soybean in Hokkaido region.
Nakamura Takuji, Watanabe Toshihiro, Chonan Yuya, Abstracts of Meeting of the CSSJ, 245, 177, 2018
CROP SCIENCE SOCIETY OF JAPAN, Japanese

トマトの染色体断片置換系統IL8-3を用いた尻腐れ発症機構の解明
渡邊涼太郎, 富崎亮太, 丸山隼人, 金山喜則, 渡部敏裕, 日本植物生理学会年会(Web), 59th, 2018

P4-3-2 アルミニウム集積植物Melastoma malabathricumのアルミニウムストレスに対する初期応答（ポスター，4-3 植物の有害元素，2018年度神奈川大会）
吉井 健祐, 丸山 隼人, 西田 翔, 和崎 淳, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 64, 0, 69, 69, 2018
一般社団法人 日本土壌肥料学会, Japanese

P4-2-5 窒素欠乏下で栽培したコムギのモリブデン集積特性（ポスター，4-2 植物の微量栄養素，2018年度神奈川大会）
徳長 そよ香, 丸山 隼人, 岡田 僚介, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 64, 0, 64, 64, 2018
一般社団法人 日本土壌肥料学会, Japanese

4-1-5 日本在来種ヤマモガシのクラスター根におけるリン可給化能（4-1 植物の多量栄養素，2018年度神奈川大会）
岡村 惟史, 渡部 敏裕, 坪田 博美, 和崎 淳, 日本土壌肥料学会講演要旨集, 64, 0, 46, 46, 2018
一般社団法人 日本土壌肥料学会, Japanese

Influence of ground rice chaffs in nursery soil on the quality of paper pots for sugar beet
大竹 勝, 渡部 敏裕, 大崎 満, 日本土壌肥料学雑誌 = Japanese journal of soil science and plant nutrition, 88, 5, 403, 412, Oct. 2017
日本土壌肥料学会 ; 1927-, Japanese

How can plant nutrition contribute to solving soil problems in tropical Asia?
小林 優, 小山 博之, 渡部 敏裕, 前島 恵理子, Sriprachote Anongnat, 落合 久美子, 清水 顕史, 近藤 始彦, 飯泉 佳子, 渡辺 武, 俵谷 圭太郎, 渡邉 彰, 日本土壌肥料学雑誌 = Japanese journal of soil science and plant nutrition, 88, 4, 346, 351, Aug. 2017
熱帯アジア地域における安定的な農業生産は，この地域に生活する人々のみならず，食料を含む各種作物の生産供給をこの地域に大きく依存している我が国にとっても，直接的に重要な課題である。今後も増加が見込まれる世界人口を支えるためには農業生産量のさらなる増加が必要である。しかし，環境への影響を考えれば，従来のような森林伐採による農耕地拡大はもはや困難であり，今後は既存農地の保全と集約的な利用，あるいは荒廃地の修復利用を図っていかなければならない。そこで，本シンポジウムではこれまで実際に熱帯アジア地域をフィールドとして研究に取り組んできた演者から，この地域の農業生産を制限する土壌肥料学的な諸問題を解説してもらうとともに，それら問題を克服する試みの実例についても話題提供を受け，持続可能な農業発展という難しい目標に対して，どのような方向で研究を進めるべきか議論した。以下に講演の概要を紹介する。, 日本土壌肥料学会 ; 1927-, Japanese

Species difference in the responses of Japanese larch and hybrid larch F₁ to O₃ exposure and ammonium sulfate
Sugai Tetsuto, Kita Kazuhito, Watanabe Toshihiro, Koike Takayoshi, The Japanese Forest Society Congress, 128, 725, 2017
[in Japanese], The Japanese Forest Society, Japanese

Nutrient dynamics of broadleaved trees grown under free-air enriched O₃ exposure
Koike Takayoshi, Shi Cong, Agathokleous Eugenios, Satoh Fuyuki, Watanabe Toshihiro, The Japanese Forest Society Congress, 128, 726, 2017
[in Japanese], The Japanese Forest Society, Japanese

トマトの染色体断片置換系統を用いた尻腐れ果抑制に関する研究
渡部敏裕, 富崎亮太, 前島恵理子, 丸山隼人, 渡邊涼太郎, 金山喜則, 渡部敏裕, 日本土壌肥料学会講演要旨集, 63, 2017

土壌難利用性リン可給化におけるルーピン根分泌性酸性ホスファターゼLASAP2と有機酸の相乗効果
古谷浩章, 丸山隼人, 渡部敏裕, 和崎淳, 日本土壌肥料学会講演要旨集, 63, 2017

P2-1-14 土壌難利用性リン可給化におけるルーピン根分泌性酸性ホスファターゼLASAP2と有機酸の相乗効果（ポスター，2-1 土壌有機・無機成分の構造・機能・ダイナミクス，2017年度仙台大会）
古谷 浩章, 丸山 隼人, 渡部 敏裕, 和崎 淳, 日土肥講要, 63, 0, 27, 27, 2017
一般社団法人 日本土壌肥料学会, Japanese

P4-1-1 トマトの染色体断片置換系統を用いた尻腐れ果抑制に関する研究（ポスター，4-1 植物の多量栄養素，2017年度仙台大会）
渡部 敏裕, 富崎 亮太, 前島 恵理子, 丸山 隼人, 渡邊 涼太郎, 金山 喜則, 渡部 敏裕, 日土肥講要, 63, 0, 56, 56, 2017
一般社団法人 日本土壌肥料学会, Japanese

P4-1-22 クラスタ一根を形成するルーピンとヤマモガシ科植物の低リン環境下での生理応答の比較（ポスター，4-1 植物の多量栄養素，2017年度仙台大会）
岡村 惟史, 花城 清俊, 丸山 隼人, 俵谷 圭太郎, 渡部 敏裕, 坪田 博美, 和崎 淳, 日本土壌肥料学会講演要旨集, 63, 0, 62, 62, 2017
一般社団法人 日本土壌肥料学会, Japanese

Relationship between the element contents of leaves in flowering stage and productivity of soybean in Hokkaido region
Nakamura Takuji, Watanabe Toshihiro, Morimoto Sho, Oka Norikuni, Tsuji Hiroyuki, Hayashi Satoshi, Chonan Yuya, Murakami Noriyuki, Sasaki Ryo, Ohashi Yuji, Abstracts of Meeting of the CSSJ, 243, 148, 2016
CROP SCIENCE SOCIETY OF JAPAN, Japanese

P4-1-18 低リン土壌に生育するヤマモガシが周辺植物のミネラル吸収に及ぼす影響（ポスター，4-1 植物の多量栄養素，2016年度佐賀大会）
岡村 惟史, 渡部 敏裕, 坪田 博美, 和崎 淳, 日本土壌肥料学会講演要旨集, 62, 0, 56, 56, 2016
一般社団法人 日本土壌肥料学会, Japanese

4-2-11 Effect of different nitrogen sources on N, mineral elements mobility and microbial metabolism in rhizosphere soil of different plant species
Chu Qingnan, Abstracts of the meeting, the Society of the Science of Soil and Manure, 61, 68, 68, 09 Sep. 2015
Japanese Society of Soil Science and Plant Nutrition, English

P4-3-8 アルミニウム超耐性をもつ木本植物の根に含まれるフェノリクスの特性(ポスター,4-3 植物の有害元素,2015年度京都大会)
前島 恵理子, 渡部 敏裕, 我妻 忠雄, 大崎 満, 日本土壌肥料学会講演要旨集, 61, 79, 79, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

4-3-11 ナトリウム処理下におけるテンサイの抗酸化応答(4-3 植物の有害元素,2015年度京都大会)
土谷 修平, 渡部 敏裕, 松本 卓也, 大崎 満, 日本土壌肥料学会講演要旨集, 61, 75, 75, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

P7-2-1 Biocharが有機質肥料を施肥した土壌の窒素動態に与える影響とその要因(ポスター,7-2 土壌改良資材,2015年度京都大会)
竹内 友規, 渡部 敏裕, 中村 隆一, 本間 千晶, 大崎 満, 日本土壌肥料学会講演要旨集, 61, 149, 149, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

6-4-1 ルーピンとの混植がイネ科牧草の生育および窒素吸収に及ぼす影響(6-4 草地土壌肥沃度,2015年度京都大会)
藤石 愛美, 渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 61, 125, 125, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

7-1-32 テンサイのカリウム施肥、リン酸施肥及び施肥栽培密度の違いが収量・根中糖分及び製糖品質に及ぼす影響について(7-1 肥料および施肥法,2015年度京都大会)
鷹田 秀一, 渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 61, 137, 137, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese

17 ナトリウムに対する生育応答の異なるテンサイの選抜(北海道支部講演会,2014年度各支部会)
松本 卓也, 田口 和憲, 大崎 満, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 61, 0, 242, 2015
一般社団法人 日本土壌肥料学会, Japanese

8-1-25 土壌に蓄積する有機態リンの主要な形態である難溶性フィチン酸塩の放射性セシウムイオン吸着特性(8-1 環境保全,2015年度京都大会)
海野 佑介, 高雄 惇英, 江口 哲也, 武田 晃, 渡部 敏裕, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 61, 0, 160, 160, 2015
一般社団法人 日本土壌肥料学会, Japanese

25 有機物連用土壌および慣行土壌で栽培したミズナとその土壌の成分に与える有機物施肥の影響(北海道支部講演会,2013年度各支部会)
大熊 直生, 渡部 敏裕, 岡崎 圭毅, 大崎 満, 日本土壌肥料学会講演要旨集, 60, 250, 250, 09 Sep. 2014
一般社団法人日本土壌肥料学会, Japanese

P4-3-10 イネの塩害に対する生育評価および遺伝学的解析(ポスター,4-3 植物の有害元素,2014年度東京大会)
清水 克, 石黒 聖也, 渡部 敏裕, 大崎 満, 貴島 祐治, 日本土壌肥料学会講演要旨集, 60, 77, 77, 09 Sep. 2014
一般社団法人日本土壌肥料学会, Japanese

P4-3-5 異なるアルミニウム集積植物におけるアルミニウムの集積形態と分布(ポスター,4-3 植物の有害元素,2014年度東京大会)
前島 恵理子, 渡部 敏裕, 平舘 俊太郎, Jansen Steven, 大崎 満, 日本土壌肥料学会講演要旨集, 60, 75, 75, 09 Sep. 2014
一般社団法人日本土壌肥料学会, Japanese

4-4-5 有機物連用土壌で栽培されたミズナの成分の特性とその変動要因(4-4 植物の代謝成分と農作物の品質,2014年度東京大会)
大熊 直生, 渡部 敏裕, 岡崎 圭毅, 岡 紀邦, 大崎 満, 日本土壌肥料学会講演要旨集, 60, 60, 81, 81, 09 Sep. 2014
一般社団法人日本土壌肥料学会, Japanese

有機物施用土壌で栽培した作物の根圏環境評価受容性
大和田野昌子, 渡部敏裕, 海野佑介, 大崎満, 日本土壌肥料学会講演要旨集, 60, 60, 248, 248, 09 Sep. 2014
一般社団法人日本土壌肥料学会, Japanese

20 有機物施用土壌で栽培した作物の根圏環境評価受容性(北海道支部講演会,2013年度各支部会)
大和田野, 昌子, 渡部 敏裕, 海野 佑介, 大崎 満, 日土肥講要, 60, 0, 248, 248, 2014
一般社団法人 日本土壌肥料学会, Japanese

Plant minerals and metabolites analysis for the improvement of plant productivity
Nakamura Takuji, Hayashi Satoshi, Murakami Noriyuki, Tsuji Noriyuki, Komatsu Kunihiko, Okazaki Keiki, Sha Zimin, Qingnan Chu, Watanabe Toshihiro, Shinano Takuro, Abstracts of Meeting of the CSSJ, 237, 0, 2014
CROP SCIENCE SOCIETY OF JAPAN, Japanese

P4-2-2 塩塚高原に生育する各種植物の土壌環境の違いによるイオノーム変動(ポスター,4-2 植物の微量栄養素,2014年度東京大会)
渡部 敏裕, 楠本 良延, 森田 沙綾香, 小柳 知代, 大崎 満, 平舘 俊太郎, 日本土壌肥料学会講演要旨集, 60, 0, 65, 65, 2014
一般社団法人 日本土壌肥料学会, Japanese

4-2-6 Ionome analysis of different organs in soybean at different growth stages in response to different nitrogen sources
Chu Qingnan, Sha Zhimin, Abstracts of the Annual Meetings, Japanese Society of Soil Science and Plant Nutrition, 60, 0, 62, 62, 2014
Japanese Society of Soil Science and Plant Nutrition, English

4-4-7 大豆の開花期における展開葉元素含有率とその生産性との関係(4-4 植物の代謝成分と農作物の品質,2014年度東京大会)
中村 卓司, 山田 哲也, 島田 信二, 村上 則幸, 林 怜史, 辻 博之, 小松 邦彦, 渡部 敏裕, Chu Qingnan, 岡崎 圭毅, 岡 紀邦, 日本土壌肥料学会講演要旨集, 60, 0, 82, 82, 2014
一般社団法人 日本土壌肥料学会, Japanese

P8-1-26 異なる施肥条件での長期連用による土壌の物理化学性の変化に起因した土壌中Cs動態及び植物体へのCs移行量変化の解析(ポスター,8-1 環境保全,2014年度東京大会)
高雄 惇英, 海野 佑介, 渡部 敏裕, 大崎 満, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 60, 0, 161, 161, 2014
一般社団法人 日本土壌肥料学会, Japanese

32 Impacts of previous cropping with mycorrhizal plant and manure application on the ionome of soybean seed
Sha Zhimin, Oka Norikuni, Watanabe Toshihiro, Okazaki Keiki, Osaki Mitsuru, Shinano Takuro, Abstracts of the meeting, the Society of the Science of Soil and Manure, 59, 248, 248, 11 Sep. 2013
Japanese Society of Soil Science and Plant Nutrition, English

Salt tolerance conferred by a plant endogenous peptide elicitor
Doman K, Watanabe T, Fujino K, Yamaguchi Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 54, 0, 23, 24, 2013
Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, Japanese

Potential of basic researches in plant nutrition for better agroproduction
KOYAMA Hiroyuki, KOBAYASHI Yuriko, TAKAHASHI Michiko, SHINANO Takuro, OHKAMA-OHTSU Naoko, MARUYAMA-NAKASHITA Akiko, MIWA Kyoko, KOBAYASHI Masaru, WATANABE Toshihiro, UENO Daisei, NAKAMURA Shin-ichi, Japanese Journal of Soil Science and Plant Nutrition, 84, 2, 136, 141, 2013
Japanese Society of Soil Science and Plant Nutrition, Japanese

P4-3-1 強酸性土壌に生育する植物の根細胞成分の特性(4-3 植物の有害元素)
前島 恵理子, 渡部 敏裕, 我妻 忠雄, 大崎 満, 日本土壌肥料学会講演要旨集, 59, 0, 82, 82, 2013
一般社団法人 日本土壌肥料学会, Japanese

P4-4-3 野菜のイオノーム解析(4-4 植物の代謝成分と農作物の品質)
渡部 敏裕, 吉村 誠子, 前島 恵理子, 山内 愛子, 大和田野 昌子, 岡田 遼介, 信濃 卓郎, 大崎 満, 浦山 勝, 日本土壌肥料学会講演要旨集, 59, 0, 90, 90, 2013
一般社団法人 日本土壌肥料学会, Japanese

35 窒素欠乏下における作物のモリブデン集積特性(北海道支部講演会,2012年度各支部会)
岡田 遼介, 渡部 敏裕, 浦山 勝, 海野 佑介, 信濃 卓郎, 大崎 満, 日本土壌肥料学会講演要旨集, 59, 0, 2013
一般社団法人 日本土壌肥料学会, Japanese

植物種によるセシウム吸収の差違
渡部敏裕, 東隆行, 日本植物生理学会年会要旨集, 53rd, 112, 09 Mar. 2012
Japanese

ミヤコグサAtMOT1相同遺伝子の機能解析
箱山雅生, DUAN Guilan, 神谷岳洋, FABIEN Lombardo, 横田圭祐, 三輪大樹, 佐藤修正, 田畑哲之, ZHENG Chen, 渡部敏裕, 信濃卓郎, 林誠, 藤原徹, 日本植物生理学会年会要旨集, 53rd, 374, 09 Mar. 2012
Japanese

P16-7 有機物施用が作物根圏に与える影響(16.畑地土壌肥よく度,2012年度鳥取大会)
大和田野, 昌子, 渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 58, 0, 120, 120, 2012
一般社団法人 日本土壌肥料学会, Japanese

11-5 イネの根脂質改変とアルミニウム耐性(11.植物の有害元素,2012年度鳥取大会)
前島 恵理子, 渡部 敏裕, 松浦 英幸, 黒田 昌治, 豊増 知伸, 岡咲 洋三, 斉藤 和季, 大崎 満, 我妻 忠雄, 日本土壌肥料学会講演要旨集, 58, 0, 74, 74, 2012
一般社団法人 日本土壌肥料学会, Japanese

12-4 代謝物プロファイリングによる有機物施用の影響評価 : ミズナで観測された有機質肥料に対する特異的な応答(12.植物の代謝成分・農作物の品質,2012年度鳥取大会)
渡邊 彩乃, 岡崎 圭毅, 渡部 敏裕, 大崎 満, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 58, 0, 89, 89, 2012
一般社団法人 日本土壌肥料学会, Japanese

IV-4 植物イオノミクスの基礎と応用(IV 植物栄養の基礎研究から見えてきた応用への可能性,シンポジウム,2012年度鳥取大会)
渡部 敏裕, 日本土壌肥料学会講演要旨集, 58, 0, 219, 219, 2012
一般社団法人 日本土壌肥料学会, Japanese

26 養分欠乏下で栽培した各種作物のイオノーム解析(北海道支部講演会,2011年度各支部会)
岡田 遼介, 渡部 敏裕, 浦山 勝, 大崎 満, 日本土壌肥料学会講演要旨集, 58, 0, 246, 246, 2012
一般社団法人 日本土壌肥料学会, Japanese

35 堆肥施用がミズナの代謝プロファイルに与える影響の解析(北海道支部講演会,2011年度各支部会)
渡邊 彩乃, 岡崎 圭毅, 福田 朋彦, 岡 紀邦, 斎藤 修平, 渡部 敏裕, 大崎 満, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 58, 0, 249, 249, 2012
一般社団法人 日本土壌肥料学会, Japanese

維管束植物種におけるセシウム集積
東隆行, 渡部敏裕, 日本植物学会大会研究発表記録, 75th, 145, 10 Sep. 2011
Japanese

35 Ionomic response of Lotus japonicusto different root-zone temperatures
QUADIR Quazi Forhad, WATANABE Toshihiro, CHEN Zheng, SHINANO Takuro, OSAKI Mitsuru, Abstracts of the meeting, the Society of the Science of Soil and Manure, 57, 297, 297, 08 Aug. 2011
Japanese Society of Soil Science and Plant Nutrition, Japanese

植物による土壌浄化の可能性と課題
渡部 敏裕, サイエンスネット, 42, 10, 13, 2011

土壌におけるセシウムの動態とファイトレメディエーション
渡部敏裕, 科学技術政策研究所講演録, 287 (Web), 2011

Plant Toxic Elements(Division 4: Plant Nutrition,Progress Review)
Shinano T, Watanabe T, Japanese Journal of Soil Science and Plant Nutrition, 82, 6, 504, 510, 2011
Japanese Society of Soil Science and Plant Nutrition, Japanese

22-7 植物のセシウムおよびストロンチウム集積特性の解析(22.環境保全)
渡部 敏裕, 東 隆行, 岡田 遼介, 信濃 卓郎, 浦山 勝, 大崎 満, 日本土壌肥料学会講演要旨集, 57, 0, 152, 152, 2011
一般社団法人 日本土壌肥料学会, Japanese

イネからの根分泌タンパク質のプロテオーム解析
信濃卓郎, 吉村誠子, KONG Fan‐Jiang, 徳竹俊二, 小松節子, 渡部敏裕, 大崎満, 和崎淳, 日本植物生理学会年会要旨集, 51st, 349, 12 Mar. 2010
Japanese

Responses of mineral stress in higher plants
Makino Amane, YAMAYA Tomoyuki, KAMATA Atsushi, OCHIAI Kumiko, KOYAMA Hiroyuki, MA Jian Feng, WATANABE Toshihiro, Japanese Journal of Soil Science and Plant Nutrition, 81, 2, 181, 189, 2010
第4部門でのシンポジウム企画の目的を述べる前に、当該部門における部会再編の経過を説明する。第4部門では、かねてより、植物栄養学研究の進展と成果を共有したいと言う要望が多く、現在の部会編成が必ずしもその要望に答えるものではない、という意見があった。一方、一つの部会「植物の無機栄養」が極端に肥大化してしまい、一部門の年会発表総数が部門総発表数の60％を超える事態も生じていた。そんな中、2007年の東京大会では、そのような会員の要望に応えて第4部門の一会場開催も試みた。この試み概ね高い評価を受け、継続の要望も多かったが、諸般の事情により2008年の名古屋大会では継続されなかった。また、今年度の京都大会においては、会場の都合上、第4部門一会場開催は不可能となった。, Japanese Society of Soil Science and Plant Nutrition, Japanese

11-12 モエジマシダのヒ素耐性における硫黄の重要性(11.植物の有害元素,2010年度北海道大会)
皇甫 蘭, 渡部 敏裕, 小野寺 とも, 中村 考志, 山口 紀子, 北島 信行, 大崎 満, 日本土壌肥料学会講演要旨集, 56, 0, 78, 78, 2010
一般社団法人 日本土壌肥料学会, Japanese

11-19 根端細胞膜脂質を改変してアルミニウム耐性植物を創る(2)(11.植物の有害元素,2010年度北海道大会)
我妻 忠雄, 小山 博之, 渡部 敏裕, 豊増 知伸, 黒田 昌治, 大山 清, 村中 俊哉, 石川 明史, 神成 貴之, 村山 哲也, 日本土壌肥料学会講演要旨集, 56, 0, 81, 81, 2010
一般社団法人 日本土壌肥料学会, Japanese

16-5 長期継続三要素試験圃場の土壌とそこで栽培したトウモロコシが含む元素の一斉分析(16.畑地土壌肥よく度,2010年度北海道大会)
渡部 敏裕, 岡田 遼介, 浦山 勝, 大崎 満, 日本土壌肥料学会講演要旨集, 56, 0, 114, 114, 2010
一般社団法人 日本土壌肥料学会, Japanese

22 ヒ素ストレスに対する植物の代謝応答解析(北海道支部講演会,2009年度各支部会)
中村 考志, 渡部 敏裕, 岡崎 圭毅, 大崎 満, 信濃 卓郎, 日本土壌肥料学会講演要旨集, 56, 0, 245, 245, 2010
一般社団法人 日本土壌肥料学会, Japanese

P10-5 Characterization of a Lotus japonicus mutant showing strong reduction in Molybdenum accumulation
Lombardo Fabien, Miwa Hiroki, Sato Shusei, Tabata Satoshi, Chen Zheng, Watanabe Toshihiro, Shinano Takuro, Fujiwara Toru, Abstracts of the meeting, the Society of the Science of Soil and Manure, 55, 88, 88, 15 Sep. 2009
Japanese Society of Soil Science and Plant Nutrition, English

イオノーム解析を用いた栄養吸収変異体の獲得方法
信濃卓郎, 岡崎圭毅, ZHENG Chen, 渡部敏裕, 研究成果情報 北海道農業, 2008, 212, 213, 30 Jun. 2009
Japanese

9-16 シロバナルーピン由来酸性ホスファターゼ及びフィターゼ過剰発現タバコにおける土壌中有機態リンの利用(9.植物の多量栄養素,2009年度京都大会)
丸山 隼人, 渡部 敏裕, 信濃 卓郎, 和崎 淳, 大崎 満, 日本土壌肥料学会講演要旨集, 55, 0, 70, 70, 2009
一般社団法人 日本土壌肥料学会, Japanese

P11-14 モエジマシダのヒ素耐性メカニズム : チオール化合物の寄与(ポスター紹介,11.植物の有害元素,2009年度京都大会)
酒井 有希, 渡部 敏裕, 中村 孝志, 石川 覚, 平舘 俊太郎, 信濃 卓郎, 大崎 満, 日本土壌肥料学会講演要旨集, 55, 0, 102, 102, 2009
一般社団法人 日本土壌肥料学会, Japanese

P20-1 イネ科植物における有機質資材からの窒素獲得(ポスター紹介,20.土壌改良資材,2009年度京都大会)
横手 美彌, 櫻井 道彦, 榎本 匠, 信濃 卓郎, 関 一人, 岸野 正典, 佐藤 真由美, 大崎 満, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 55, 0, 162, 162, 2009
一般社団法人 日本土壌肥料学会, Japanese

II-7 好酸性植物とアルミニウム(II 植物のミネラルストレス応答,シンポジウム,2009年度京都大会)
渡部 敏裕, 日本土壌肥料学会講演要旨集, 55, 0, 219, 219, 2009
一般社団法人 日本土壌肥料学会, Japanese

11 無菌条件下におけるイノシン施与が根の生長に与える影響(北海道支部講演会,2008年度各支部会)
トクヒサ ダイ, 山村 卓也, 信濃 卓郎, 渡部 敏裕, 横井 大輔, 大崎 満, 日本土壌肥料学会講演要旨集, 55, 0, 246, 246, 2009
一般社団法人 日本土壌肥料学会, English

モエジマシダのヒ素無毒化機構におけるγグルタミルシステイン合成酵素の役割
酒井有希, 渡部敏裕, 和崎淳, 瀬野浦武志, 信濃卓郎, 大崎満, 日本植物生理学会年会要旨集, 49th, 109, 15 Mar. 2008
Japanese

熱帯泥炭より分離した新規(+)‐catechin資化細菌Burkholderia sp.KTC‐1による(+)‐catechinのtaxifolinへの変換
松田元規, 大塚祐一郎, 神繁樹, 和崎淳, 和崎淳, 渡辺純, 渡部敏裕, 大崎満, 日本農芸化学会大会講演要旨集, 2008, 265, 05 Mar. 2008
Japanese

A study of a novel integrated myocardial management associated with Microplegia
Watanabe Satoru, Chiba Fumio, Nasu Toshihiro, Sugawara Seiichi, Nemoto Takafumi, Furukawa Hirokazu, Japanese Journal of Extra-Corporeal Technology, 35, 2, 138, 141, 2008
要旨連合型心筋保護法(Integrated Myocardial Management:IMM)にMicroplegiaを併用した開心術症例について、従来の心筋保護法を実施した症例と比較検討し、報告する。対象は2006年9月から2007年5月までの成人開心術症例のうち、IMMのみ行った15例(I群)とIMMにMicroplegiaを併用した15例(M群)とした。その結果、ICU帰室後CPK-MB値、心肺血K値(max)などでそれぞれ統計学的有意差は認められなかった。しかし、cold blood灌流時に心室細動が発生し、それに対し心筋保護液が過剰投与されることによるoverdoseを懸念し、最低限のカリウム濃度で灌流中に弛緩性心停止を維持できるMicroplegiaを併用する必要があった。そのため、MPSを使用しIMMにcold bloodにカリウムを添加して灌流するMicroplegiaを併用した心筋保護法は、血液灌流時に6～8mEq/Lのカリウム濃度を維持することが可能であることにより、確実な心停止が得られる方法として有用であると考えられた。, The Japanese Society of Extra-Corporeal Technology in Medicine, Japanese

Development of Catalytic Membrane-Installed Microreaction Devices
Yamada Yoichi M. A., Watanabe Toshihiro, Uozumi Yasuhiro, Fukuyama Naoshi, Beppu Tomohiko, Torii Kaoru, Proceedings of the Symposium on Progress in Organic Reactions and Syntheses, 34, 178, 178, 2008
Instantaneous C-C bond forming reactions were achieved by using a microchannel reactor bearing catalytic membrane inside the channel. Thus, the catalytic membrane PA-TAP-Pd was constructed inside the microchannel in a "ship-in-a-bottle" fashion via molecular convolution of an non-crosslinked polymeric phosphine ligand and (NH4)2PdCl4 at 25 C for 10 min at the interface between EtOAc and aqueous phases flowing laminarly, where an non-crosslinked polymer-bound phosphine and (NH4)2PdCl4 dissolved, respectively. The palladium-catalyzed Suzuki-Miyaura reaction of aryl halides in EtOAc/i-C3H7OH and arylboronic acids in aqueous Na2CO3 at 50 C was performed using the microreaction device to afford the corresponding biaryl compounds in 96-99% yield within 4 sec of retention time in the defined channel region. The microdevice system was also successfully applied to arylation of allylic esters to give allylarenes with retention time of 1 sec., Division of Organic Chemistry, The Pharmaceutical Society of Japan, Japanese

01-012 Analysis of diazotroph diversity associated with the rhizosphere of Melastoma malabathricum L.(Soil ecosystem)
Sato Atsuya, Shinano Takuro, Watanabe Toshihiro, Purnomo Erry, Osaki Mitsuru, 日本微生物生態学会講演要旨集, 24, 64, 64, 2008
日本微生物生態学会, Japanese

9-1 Ionomics study of Lotus japonicus reveals the element homeostasis network in plants
Quadir Quazi Forhad, Abstracts of the Annual Meetings, Japanese Society of Soil Science and Plant Nutrition, 54, 0, 70, 70, 2008
Japanese Society of Soil Science and Plant Nutrition, English

9-27 モエジマシダのヒ素無毒化機構におけるチオールペプチドの役割について(9.植物の無機栄養,2008年度愛知大会)
酒井 有希, 渡部 敏裕, 和崎 淳, 瀬野浦 武志, 信濃 卓郎, 大崎 満, 日本土壌肥料学会講演要旨集, 54, 0, 78, 78, 2008
一般社団法人 日本土壌肥料学会, Japanese

9-45 シロバナルーピン由来酸性ホスファターゼアイソザイムの発現とプロモーター領域の解析(9.植物の無機栄養,2008年度愛知大会)
丸山 隼人, 田中 美穂, 和崎 淳, 瀬野浦 武志, 渡部 敏裕, 信濃 卓郎, 伊藤 進, 大崎 満, 日本土壌肥料学会講演要旨集, 54, 0, 84, 84, 2008
一般社団法人 日本土壌肥料学会, Japanese

11-10 根圏プロテアーゼに着目した、植物の有機態窒素利用の検討(11.植物の栄養生態,2008年度愛知大会)
榎本 匠, 渡部 敏裕, 岡本 美輪, 櫻井 道彦, 信濃 卓郎, 大崎 満, 日本土壌肥料学会講演要旨集, 54, 0, 106, 106, 2008
一般社団法人 日本土壌肥料学会, Japanese

P20-4 ホウレンソウのヒ素及びカドミウム吸収に対する零価鉄施与の効果(ポスター紹介,20.土壌改良資材,2008年度愛知大会)
中村 考志, 渡部 敏裕, 酒井 有希, 村田 泰利, 大崎 満, 日本土壌肥料学会講演要旨集, 54, 0, 164, 164, 2008
一般社団法人 日本土壌肥料学会, Japanese

6 カドミウム耐性を有する微生物による植物生長への影響(北海道支部講演会,2007年度各支部会)
野村 和弘, 海野 佑介, 渡部 敏裕, 松田 元規, 大崎 満, 日本土壌肥料学会講演要旨集, 54, 0, 242, 242, 2008
一般社団法人 日本土壌肥料学会, Japanese

8-20 アーバスキュラー菌根共生によるAl感受性植物の耐酸性付与のメカニズム(8.共生,2007年度東京大会)
稲葉 尚子, 渡部 敏裕, 大崎 満, 江沢 辰広, 日本土壌肥料学会講演要旨集, 53, 0, 58, 58, 2007
一般社団法人 日本土壌肥料学会, Japanese

P9-33 Ion-profile variations of Lotus japonicus wild lines under 9 elements treatment in subtoxic levels
Zheng Chen, Abstracts of the Annual Meetings, Japanese Society of Soil Science and Plant Nutrition, 53, 0, 87, 87, 2007
Japanese Society of Soil Science and Plant Nutrition, English

P9-43 シダ植物の元素集積特性(9.植物の無機栄養,2007年度東京大会)
渡部 敏裕, Jansen Steven, 大崎 満, 日本土壌肥料学会講演要旨集, 53, 0, 90, 90, 2007
一般社団法人 日本土壌肥料学会, Japanese

酸性土壌に生育する植物たち : アルミニウム集積植物に見る多彩な適応のメカニズム
渡部 敏裕, 化学と生物, 44, 7, 496, 499, 01 Jul. 2006
日本農芸化学会, Japanese

8-2 Al超集積性植物 Melastoma spp. (ノボタン属植物) の生育およびAl吸収におけるアーバスキュラー菌根共生の役割(8. 共生, 2006年度秋田大会講演要旨)
稲葉 尚子, 渡部 敏裕, 大崎 満, 江沢 辰広, 日本土壌肥料学会講演要旨集, 52, 0, 41, 41, 2006
一般社団法人 日本土壌肥料学会, Japanese

P9-2 Melastoma malabathricum の根が分泌するムシラゲの性質に関する研究(9. 植物の無機栄養, 2006年度秋田大会講演要旨)
見澤 誠司, 渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 52, 0, 74, 74, 2006
一般社団法人 日本土壌肥料学会, Japanese

P9-6 酸性硫酸塩土壌優占種の生育におけるAlとFeの相互作用(9. 植物の無機栄養, 2006年度秋田大会講演要旨)
渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 52, 0, 75, 75, 2006
一般社団法人 日本土壌肥料学会, Japanese

11-6 葉菜類におけるアミノ酸吸収とその動態(11. 植物の栄養生態, 2006年度秋田大会講演要旨)
岡本 美輪, 明石 崇, 大崎 満, 渡部 敏裕, 日本土壌肥料学会講演要旨集, 52, 0, 92, 92, 2006
一般社団法人 日本土壌肥料学会, Japanese

22-9 零価鉄施与がイネのカドミウム吸収および土壌中カドミウムの形態に与える影響(22. 環境保全, 2006年度秋田大会講演要旨)
村田 泰利, 渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 52, 0, 163, 163, 2006
一般社団法人 日本土壌肥料学会, Japanese

9-15 Melastoma malabathricumの根が分泌するムシラゲのアルミニウム吸収における役割(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)
見澤 誠司, 渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 51, 0, 62, 62, 2005
一般社団法人 日本土壌肥料学会, Japanese

9-16 Melastoma malabathricumの根が分泌するムシラゲの特性(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)
渡部 敏裕, 見澤 誠司, 平舘 俊太郎, 大崎 満, 日本土壌肥料学会講演要旨集, 51, 0, 63, 63, 2005
一般社団法人 日本土壌肥料学会, Japanese

9-27 重金属汚染土壌を修復するための新たな植物種の選定(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)
村田 泰利, 渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 51, 0, 66, 66, 2005
一般社団法人 日本土壌肥料学会, Japanese

Rhizosphere Management : From Analysis of Rhizosphere Function to Its Control
NISHIYAMA Masaya, HASHIDOKO Yasuyuki, EZAWA Tatsuya, OHSAKI Mitsuru, ZHANG Fusuo, SAITO Masanori, Japanese journal of soil science and plant nutrition, 75, 3, 405, 410, 05 Jun. 2004
日本土壌肥料学会, Japanese

ルーピンの有機態窒素利用特性の解析(11. 植物の栄養生態, 2004年度大会講演要旨集)
渡部 敏裕, 見澤 誠司, 浦山 勝, 大崎 満, 日本土壌肥料学会講演要旨集, 50, 0, 103, 103, 2004
一般社団法人 日本土壌肥料学会, Japanese

9-18 低pH条件下で多価カチオンがイネの根伸長に与える影響 : アルミニウムイオンを中心に(9.植物の無機栄養)
渡部 敏裕, 岡田 謙介, 日本土壌肥料学会講演要旨集, 49, 0, 70, 70, 2003
一般社団法人 日本土壌肥料学会, Japanese

9-7 イネのアルミニウム耐性品種間差に関する研究(9.植物の無機栄養)
渡部 敏裕, 岡田 謙介, 日本土壌肥料学会講演要旨集, 48, 0, 48, 48, 2002
一般社団法人 日本土壌肥料学会, Japanese

11-11 有機態窒素に対するイネ科作物の生育反応特性(11.植物の栄養生態)
岡本 美輪, 岡田 謙介, 渡部 敏裕, 阿江 教治, 日本土壌肥料学会講演要旨集, 48, 0, 78, 78, 2002
一般社団法人 日本土壌肥料学会, Japanese

9-32 Melastoma malabathricumのAl集積と生育における有機酸の役割(9.植物の無機栄養)
渡部 敏裕, 大崎 満, 日本土壌肥料学会講演要旨集, 47, 0, 76, 76, 2001
一般社団法人 日本土壌肥料学会, Japanese

9-26 Melastoma malabathricum L.のアルミニウム集積に関する研究(9.植物の無機栄養)
渡部 敏裕, 大崎 満, 但野 利秋, 日本土壌肥料学会講演要旨集, 46, 0, 78, 78, 2000
一般社団法人 日本土壌肥料学会, Japanese

植物体内でのAlとSiの局在性 (文部省S)
佐藤純子, 渡部敏裕, 信濃卓郎, 但野利秋, 植物・土壌系におけるアルミニウムとケイ酸の相互作用の機構解明 平成8-10年度 No.08406007, 59, 69, 1999
Japanese

8 リン栄養状態を異にする作物のアルミニウム耐性(北海道支部講演会)
羅 会明, 渡部 敏裕, 信濃 卓郎, 但野 利秋, 日本土壌肥料学会講演要旨集, 45, 0, 322, 322, 1999
一般社団法人 日本土壌肥料学会, Japanese

9-26 酸性土壌に適応した木本植物の生育におけるAlと窒素源の相互作用(9.植物の無機栄養)
渡部 敏裕, 大崎 満, 但野 利秋, 日本土壌肥料学会講演要旨集, 44, 0, 77, 77, 1998
一般社団法人 日本土壌肥料学会, Japanese

Improvement of 8-Quinolinol Method to Determine Low Concentration of Aluminum under the Presence of High Iron and Copper
WATANABE Toshihiro, MIZUNO Naoharu, TADANO Toshiaki, Japanese Journal of Soil Science and Plant Nutrition, 68, 4, 441, 443, 1997
Japanese Society of Soil Science and Plant Nutrition, Japanese

9-18 Al集積植物体内におけるAlの分配(9.植物の無機栄養)
渡部 敏裕, 大崎 満, 吉原 照彦, 但野 利秋, 日本土壌肥料学会講演要旨集, 43, 0, 80, 80, 1997
一般社団法人 日本土壌肥料学会, Japanese

10-12 低pH土壌適応植物のAlに対する生育反応とAl吸収の特性(10.植物の無機栄養・代謝)
渡部 敏裕, 大崎 満, 但野 利秋, 日本土壌肥料学会講演要旨集, 42, 0, 73, 73, 1996
一般社団法人 日本土壌肥料学会, Japanese

10-44 好酸性および耐酸性植物の生育と養分吸収におよぼすAlとCaの施与ならびにpHの影響(10.植物の無機栄養・代謝)
渡部 敏裕, 大崎 満, 但野 利秋, 日本土壌肥料学会講演要旨集, 41, 0, 95, 95, 1995
一般社団法人 日本土壌肥料学会, Japanese

5-52 低pH土壌に生育する植物のAl耐性(5.植物の無機栄養および養分吸収)
大崎 満, 渡部 敏裕, 阿部 孝, 但野 利秋, 日本土壌肥料学会講演要旨集, 40, 0, 86, 86, 1994
一般社団法人 日本土壌肥料学会, Japanese

6-28 高CO_2がガス交換能と窒素利用効率におよぼす影響のコムギとダイズ間比較(6. 植物の代謝および代謝成分)
中村 卓司, 大崎 満, 小池 孝良, 但野 利秋, 日本土壌肥料学会講演要旨集, 39, 39, 88, 88, 25 Mar. 1993
一般社団法人日本土壌肥料学会, Japanese

Aluminum Stress Adaptation in Plants　　　　　　　　　　　　　　　
Wagatsuma T, Maejima E, Watanabe T, Khan MSH, Ishikawa S, Significant Role of the Plasma Membrane Lipid Bilayers in Aluminum Tolerance of Plants
Springer, 2015, 99-124, [Joint work]

園芸学の基礎　　　　　　　　　　　　　　　
Toshihiro Watanabe, 養分吸収の生理
農文協, 2012, 109-118, [Joint work]

Principles, Application and Assessment in Soil Science　　　　　　　　　　　　　　　
Watanabe T, Khan MSH, Rao IM, Wasaki J, Shinano T, Ishitani M, Koyama H, Ishikawa S, Tawaraya K, Nanamori N, Ueki N, Wagatsuma T, Physiological and Biochemical Mechanisms of Plant Adaptation to Low-Fertility Acid Soils of the Tropics: The Case of Brachiariagrasses
IntechOpen, 2011, [Joint work]

Genetics, Genomics, and Breeding of Soybean　　　　　　　　　　　　　　　
Nakamura T, Okazaki K, Benkeblia N, Wasaki J, Watanabe T, Matsuura H, Uchimiya H, Komatsu S, Shinano T, Metabolomics approach in soybean
CRC Press, 2010, 313-330, [Joint work]

北海道農業と土壌肥料2010　　　　　　　　　　　　　　　
櫻井道彦, 渡部敏裕, 土壌有機物の動態解析法
北農会, 2010, 253-255, [Joint work]

The Evolution of Plant Physiology. From whole plant to ecosystems　　　　　　　　　　　　　　　
Jansen S, Watanabe T, Dessein S, Robbrecht E, Smets E, The evolution of aluminium accumulation in angiosperms
Elsevier Academic Press, 2004, 467-479, [Joint work]

Sustainable Agriculture for Food, Energy, and Industry　　　　　　　　　　　　　　　
Osaki M, Matsumoto M, Watanabe T, Kawamukai T, Shinano T, Nuyim T, Nilnond C, Tadano T, Strategies for adaptation of plants grown in adverse soils
James & James Ltd., 1998, 537-546, [Joint work]

Response of plants adapted in low pH soils to aluminum　　　　　　　　　　　　　　　
Watanabe T, Osaki M, Tadano T
XIII International Plant Nutrition Colloquium
Tokyo

Growth stimulation effect of aluminum on Al accumulator plant - Melastoma malabathricum　　　　　　　　　　　　　　　
Watanabe T, Osaki M, Damdinsuren T, Tadano T
International Workshop on New Concepts of Plant Nutrient Acquisition
Tsukuba

Effect of aluminum on growth of melastoma (Melastoma malabathricum L.)　　　　　　　　　　　　　　　
Watanabe T, Osaki M, Tadano T
International Symposium on Impact of Potential Tolerance of Plants on the Increased Productivity under Aluminum Stress
Okayama

Study on aluminum resistance in relation to organic acid anion exudation from roots of PEPC transgenic rice plants　　　　　　　　　　　　　　　
Osaki M, Nursymsi D, Begum HH, Watanabe T
XIV International Plant Nutrition Colloquium
Germany

A physiological study of Melastoma malabathricum, an aluminium accumulating woody plant　　　　　　　　　　　　　　　
Watanabe T, Jansen S, Osaki M
Fifth Keele Meeting on Aluminium. Aluminium in Life: from acid rain to Alzheimer's disease,
U.K.

The distribution, ecology and evolution of aluminium accumulation in flowering plants　　　　　　　　　　　　　　　
Jansen S, Watanabe T, Smets E
Fifth Keele Meeting on Aluminium. Aluminium in Life: from acid rain to Alzheimer's disease,
U.K.

The relationship between growth and Al accumulation in Melastoma malabathricum L., an Al accumulator growing in tropical acid soils　　　　　　　　　　　　　　　
Watanabe T, Jansen S, Osaki M
6th International Symposium on Plant-Soil Interactions at Low pH
Sendai

Variation in leaf mineral concentrations of terrestrial plants in Japan　　　　　　　　　　　　　　　
Osaki M, Watanabe T, Tuah SJ, Takada J, Satake K, Takamatsu T, Jansen S
First International Workshop on Plant Ionomics
China

Possibility of rhizosphere regulation for phytoremediation and ecosystem health　　　　　　　　　　　　　　　
Osaki M, Matsuda M, Afrida, Hoshina S, Tamai Y, Purnomo E, Watanabe T, Wasaki J, Shinano T
International Symposium on Phytoremediation and Ecosystem Health
China

Different capabilities of organic nitrogen uptake in cereal crops　　　　　　　　　　　　　　　
Okamoto M, Okada K, Watanabe T, Ae N, Osaki M
XV International Plant Nutrition Colloquium
China

The characteristics of aluminum uptake in roots of Melastoma malabathricum, an aluminum accumulating plant　　　　　　　　　　　　　　　
Watanabe T, Misawa S, Hiradate S, Jansen S, Osaki M
XV International Plant Nutrition Colloquium
China

Mechanism underlying improved plant growth in acidic soil by arbuscular mycorrhizal symbiosis　　　　　　　　　　　　　　　
Inaba S, Watanabe T, Osaki M, Ezawa T
Second International Rhizosphere Conference
France

Different characteristics of root mucilage in aluminium adsorption between Melastoma malabathricum and Zea mays　　　　　　　　　　　　　　　
Watanabe T, Misawa S, Hiradate S, Osaki M
Second International Rhizosphere Conference
France

Difference in acquisition of soil organic nitrogen between pak-choi and tomato　　　　　　　　　　　　　　　
Watanabe T, Enomoto T, Okamoto M, Sakurai M, Takuro S, Osaki M
International Plant Nutrition Colloquium XVI
U.S.A.

Role of thiol compounds in arsenic tolerance in Pteris vittata　　　　　　　　　　　　　　　
Watanabe T, Yuki S, Senoura T, Hiradate S, Wasaki J, Osaki M
International Plant Nutrition Colloquium XVI
U.S.A.

Decrease of cadmium accumulation in crops by zero-valent iron　　　　　　　　　　　　　　　
Watanabe T, Nakamura T, Murata Y, Sakai Y, Osaki M
International Plant Nutrition Colloquium XVI
U.S.A.

Possible reasons why aluminum is a beneficial element for Melastoma malabathricum, an aluminum accumulator　　　　　　　　　　　　　　　
Watanabe T, Osaki M
International Plant Nutrition Colloquium XVI
U.S.A.

Rice root growth with increasing in plant hormone and allantoin by inosine in nutrient solution　　　　　　　　　　　　　　　
Tokuhisa D, Okazaki K, Shinano T, Watanabe T, Yokoi D, Osaki M
International Plant Nutrition Colloquium XVI
U.S.A.

Comprehensive analysis of mineral elements in different plant species under N, P and K deficiencies　　　　　　　　　　　　　　　
Okada R, Watanabe T, Urayama M, Osaki M
Rhizosphere 3 International Conference
Australia

Plasma membrane sterols and the related gene in root-tip portion as key components for aluminium tolerance of varieties of plants species, cultivars, lines and mutants　　　　　　　　　　　　　　　
Wagatsuma T, Watanabe T, Khan MSH, Sekimoto H, Yokota T, Nakano T, Toyomasu T, Tawaraya K, Koyama H
Rhizosphere 3 International Conference
Australia

Uptake and distribution of sulphur in Pteris vittata grown with arsenic　　　　　　　　　　　　　　　
Watanabe T, Kouho R, Onodera T, Kitajima N, Sakamoto N, Shinano T, Yurimoto H, Osaki M
Rhizosphere 3 International Conference
Australia

Nano LC-MS analysis on root secreting　　　　　　　　　　　　　　　
Shinano T, Yoshimura T, Komatsu S, Tokutake S, Kong FJ, Watanabe T, Osaki M
8th Symposium of the International Society of Root Research
U.K.

HMG is a promising key gene for the enhancement of aluminum tolerance of rice via modulating membrane dterols in toot-yip portion　　　　　　　　　　　　　　　
Wagatsuma T, Watanabe T, Toyomasu T, Kuroda M, Muranaka T, Ohyama K, Ishikawa A, Usui M, Maejima E, Khan MSH, Tawaraya K, Koyama H
International Plant Nutrition Colloquium
Turkey

Characterization of nickel and zinc tolerance, accumulation and mineral profile in the metal hyperaccumulator Noccaea cochleariforme　　　　　　　　　　　　　　　
Nishida S, Yoshida J, Watanabe T, Fujiwara T, Miozuno T
International Plant Nutrition Colloquium
Turkey

Ionomics study of fertilizer responses in soybean shoots　　　　　　　　　　　　　　　
Sha Z, Watanabe T, Ohira K, Okazaki K, Osaki M, Shinano T
International Plant Nutrition Colloquium
Turkey

Effects of Organic matter application on the dynamics of organic & inorganic compounds, & microbial activities in Rhizosphere　　　　　　　　　　　　　　　
Owadano M, Watanabe T, Osaki M
International Plant Nutrition Colloquium
Turkey

Accumulation of non-essential elements in pteridophytes with emphasis on aluminum　　　　　　　　　　　　　　　
Watanabe T, Jansen S, Maejima E, Azuma T, Hiradate S, Osaki M
International Plant Nutrition Colloquium
Turkey

Phosphorus deficiency enhances the tolerance of rice to the acidic soil stress　　　　　　　　　　　　　　　
Maejima E, Watanabe T, Osaki M, Wagatsuma T
International Plant Nutrition Colloquium
Turkey

Characteristics of arsenic accumulation in aquatic plants　　　　　　　　　　　　　　　
Yamauchi A, Watanabe T, Osaki M
International Plant Nutrition Colloquium
Turkey

Physiological responses of Hakea laurina (Proteaceae) to low-P and high-P conditions　　　　　　　　　　　　　　　
Maruyama H, Yamauchi T, Kohama T, Watanabe T, Wasaki J
5th Phosphorus in Soils and Plants Symposium
France

Varietal difference in the radioactive cesium uptake in Amaranthus and soybean　　　　　　　　　　　　　　　
Shinano T, Chu Q, Watanabe T, Matsunami H, Murakami T, Kobayashi D, Okouchi T
13th International Conference on the Biogeochemistry of Trace Elements
Fukuoka

Adsorption of radiocesium by metal phytate, major component of soil organic phosphorus　　　　　　　　　　　　　　　
Unno Y, Takao A, Eguchi T, Takeda A, Watanabe T, Shinano T
13th International Conference on the Biogeochemistry of Trace Elements
Fukuoka

Effect of phosphorus status on distribution of other elements in Hakea laurina (Proteaceae)　　　　　　　　　　　　　　　
Maruyama H, Kohama T, Yamauchi T, Watanabe T, Wasaki J
13th International Conference on the Biogeochemistry of Trace Elements
Fukuoka

Comparative analysis of aluminium accumulation in leaves of several woody aluminium accumulators　　　　　　　　　　　　　　　
Maejima E, Watanabe T, Hiradate S, Jansen S, Osaki M
The Eleventh Keele Meeting on Aluminium
France

Differences and similarities in the characteristics of aluminium accumulation in various aluminium accumulators　　　　　　　　　　　　　　　
Watanabe T, Maejima E, Hiradate S, Osaki M, Jansen S
The Eleventh Keele Meeting on Aluminium
France

The characteristics of root cell components of plants adapted to acidic soil　　　　　　　　　　　　　　　
Maejima E, Watanabe T, Wagatsuma T, Osaki M
Rhizosphere 4
The Netherlands

Effect of different nitrogen sources on nitrogen dynamics, mineral elements and microbial communities in rhizosphere soil of different plant species　　　　　　　　　　　　　　　
Chu Q, Watanabe T, Sha Z, Osaki M
Rhizosphere 4
The Netherlands

Ionomic variation in plant species growing in various soil environments　　　　　　　　　　　　　　　
Watanabe T, Kusumoto Y, Morita S, Koyanagi T, Osaki M, Hiradate S
Rhizosphere 4
The Netherlands

Physiological variations in aluminum accumulation in different aluminum-accumulator plant species　　　　　　　　　　　　　　　
Watanabe T, Maejima E, Hiradate S, Wasaki J, Osaki M, Jansen S
The 9th International Symposium on Plant-Soil Interactions at Low pH
Croatia

Characteristics of root cell components in aluminum-tolerant woody plants　　　　　　　　　　　　　　　
Maejima E, Watanabe T, Wagatsuma T, Osaki M
The 9th International Symposium on Plant-Soil Interactions at Low pH
Croatia

Significant role of the plasma membrane (PM) lipid bilayers in aluminum (Al) tolerance of plants　　　　　　　　　　　　　　　
Wagatsuma T, Maejima E, Watanabe T, Khan MSH, Ishikawa S, Tawaraya K, Koyama H
The 9th International Symposium on Plant-Soil Interactions at Low pH
Croatia

Ionomic variation in leaves of 826 plant species growing in the botanical garden of Hokkaido University, Japan　　　　　　　　　　　　　　　
Watanabe T, Maejima E, Osaki M, Azuma T
Plant Biology Europe EPSO/FESPB 2016 Congress
Czech Republic

Characteristics of phenolics in root cells of Al-tolerant woody plants　　　　　　　　　　　　　　　
Maejima E, Hiradate S, Osaki M, Wagatsuma T, Watanabe T
Plant Biology Europe EPSO/FESPB 2016 Congress
Czech Republic

The mechanism of acid tolerance of Lycopodium cernuum L. grown in a solfatara field　　　　　　　　　　　　　　　
Saito T, Watanabe T, Nakatsubo T, Wasaki J
Taiwan Japan Plant Biology 2017
Taiwan

土壌及び植物栄養学概論　　　　　　　　　　　　　　　
北海道大学

物理化学　　　　　　　　　　　　　　　
北海道大学

生物共生化学　　　　　　　　　　　　　　　
北海道大学

植物栄養生態学　　　　　　　　　　　　　　　
北海道大学

生物機能化学実験II　　　　　　　　　　　　　　　
北海道大学

肥料学　　　　　　　　　　　　　　　
北海道大学

植物栄養学　　　　　　　　　　　　　　　
北海道大学

2024 - Present
植物学会　　　　　　　　　　　　　　　

2023 - Present
Sigma Xi　　　　　　　　　　　　　　　

2022 - Present
日本鉄鋼協会　　　　　　　　　　　　　　　

「植物界のシーラカンス」小葉類の極限環境突破能力
科学研究費助成事業
27 Jun. 2025 - 31 Mar. 2029
和崎 淳, 中坪 孝之, 上田 晃弘, 丸山 隼人, 草場 信, 中野 道治, 渡部 敏裕
日本学術振興会, 挑戦的研究(開拓), 広島大学, 25K21735

Technology to promote the circulation of inorganic nutrients that constitute bone through ectmycorrhizal fungi: A case study of larch forests
Grants-in-Aid for Scientific Research
27 Jun. 2025 - 31 Mar. 2028
小池 孝良, 上田 裕文, 渡部 敏裕
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, 25K22378

Evolutionary aspects of aluminum hyperaccumulation in vascular plants
Grants-in-Aid for Scientific Research
01 Apr. 2023 - 31 Mar. 2027
渡部 敏裕, 和崎 淳, 東 隆行, 佐野 雄三, 丸山 隼人, 平舘 俊太郎
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23K26801

Relationship between vascular plant evolution and aluminum hyperaccumulation
Grants-in-Aid for Scientific Research
01 Apr. 2023 - 31 Mar. 2027
渡部 敏裕, 和崎 淳, 平舘 俊太郎, 佐野 雄三, 丸山 隼人, 東 隆行
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23H02108

作物の生理障害の機構解明におけるブレークスルーテクノロジーの開発と検証
科学研究費助成事業
05 Apr. 2021 - 31 Mar. 2026
金山 喜則, 高橋 英樹, 渡部 敏裕, 須川 成利, 栗原 大輔, 黒田 理人
日本学術振興会, 基盤研究(A), 東北大学, 21H04721

Utilization of functions by cluster root formation for sustainable crop production
Grants-in-Aid for Scientific Research
05 Apr. 2021 - 31 Mar. 2025
和崎 淳, 坪田 博美, 丸山 隼人, 西田 翔, 渡部 敏裕, 佐々木 孝行, 俵谷 圭太郎, 廣田 隆一
リン酸質肥料は作物生産に必須であるが、資源は涸渇に瀕しており、持続的な作物生産にはその問題の解決が必要である。低リン耐性を有する一部の植物は、クラスター根とよばれる小根が密集した根を形成する能力がある。このクラスター根は、根の表面積を増やし、難利用性リンの吸収を強化して適応するために形成されると考えられているが、その形成能と難利用性リンの供給能の詳細は未解明である。本研究ではシステムズ生物学アプローチ解析によるクラスター根の形態形成イベントの制御、爆発的な根分泌の誘導による難利用性リンからの供給能の解明に取り組む。
これまでに、クラスター根形成種であるシロバナルーピンおよびHakea laurinaのRNA-seqデータなどを元にして複数の有機酸トランスポーター候補の同定に成功した。これらの候補遺伝子の発現量を調査したところ、低リン条件のクラスター根で顕著な発現誘導が確認された。また、シロバナルーピンのRNA-seqデータからクラスター根の発達へのエチレンの寄与が示唆されたことから、エチレン前駆体であるACCおよびエチレン合成阻害剤であるCo2+イオンを添加してその影響を評価した。その結果、エチレンはクラスター根を構成する小根の伸長停止に寄与するとともに、リン欠乏への発現応答にも関与していることが示唆された。
新規なクラスター根形成種の探索を行うため、根のメタバーコーディングライブラリー作成のためのサンプル採集を進めた。クラスター根圏土壌細菌群集のメタバーコーディングを行うため、根圏土壌を採取し、アンプリコンシーケンスの準備を進めた。シロバナルーピンの根箱栽培を実施し、クラスター根におけるリン可溶化に関わる遺伝子発現および土壌中のリン形態を調査した結果、クラスター根の発達段階だけでなく根が形成された位置による違いが認められ、局所的に異なる機能を持つことが明らかとなった。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hiroshima University, 21H04717

Large-scale X-ray fluorescence analysis on plant specimens and construction of information base on element accumulation of plants
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2020 - 31 Mar. 2024
水野 隆文, 渡部 敏裕, 橋本 篤
初年度（2020年）にXRFによる測定方法を確立し，さらに博物館，植物園および大学など植物標本を所有する学術機関と連携し，標本の貸与に関するルールを作成した。そのうえで2021年度は新たに高知県立牧野植物園，東北大学附属植物園，国立科学博物館，岡山大学資源植物科学研究所と連携し，XRFによる元素濃度のデータ収集し，日本の野生植物に関する植物栄養学知見の獲得を進めた。
特に2021年度は日本の国土の30％を占める代表的な土壌型である黒ボク土地帯と，それ以外の地域に分布する野生植物について，イオウとリンの集積レベルに関する解析を実施した。自作標本を含む1,322点の植物標本について解析した結果，次の様な結果を得た。①火山周辺の黒ボク土に分布する野生植物は，非火山灰土壌の植物と比べ統計的に有意にイオウが高く，また健全な生育に必要とされる乾燥重量あたりの濃度(0.1％）を下回った比率は標本全体で17％程度となり，日本の野生植物は火山から供給により十分なイオウ濃度を維持していることが判明した。②黒ボク土では植物のリン吸収が抑制されることが広く知られているが，植物標本の解析結果からは土壌タイプの違いによる集積量の差は認められず，一方でいずれの土壌においても全体の三分の二が健全な生育に必要なリン濃度(0.2%)を下回っていた。よって，リンについては火山灰土壌か否かではなく，土壌に含まれるリン吸着性を有する粘土鉱物（アロフェン）の含有率で比較すべきであることが示唆された。それ以外にも③イオウとリンいずれも木本植物より草本植物での集積量が有意に高い，④植物の科のなかでユリ科の植物に高いイオウ集積性が確認された。⑤植物のイオウとリンの集積量には正の相関が認められる。などのことが新たに確認されるなど，植物のイオウとリンの集積に関する重要な知見を得ることができた。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Mie University, 20K05765

Development of two-dimensional ionomics and its application to plant nutrition
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2020 - 31 Mar. 2023
渡部 敏裕, 平舘 俊太郎
昨年度はアルミニウム（Al）および鉄（Fe）処理を施した植物サンプルに含まれる元素をバッファーで抽出し、分子量で分画した後に各元素濃度を測定することを試みた。しかし元素による抽出効率の違いが大きく、分画時に吸着による損失が生じた。そこで、単一のバッファーによる抽出液を分画するのではなく、異なるバッファーで抽出される成分の元素プロファイルに対する処理の影響を調査した。+Al処理と+Fe処理をメラストーマに対して行い、処理後の各部位を中性あるいは酸性バッファーで抽出し、抽出液をICP-MSで測定した。また、サンプルを湿式分解し全濃度についても測定した。その結果、抽出バッファーの違いで傾向が異なる元素が認められた。例えば、若葉でのカルシウムは+Al処理で中性バッファーではコントロールと比べて濃度が著しく低下した一方、酸性バッファーでは大きく上昇した。この傾向は+Fe処理では認められず+Al特有の現象だった。一方、若葉のモリブデン濃度については+Al処理と+Fe処理の両方で酸性バッファーでの濃度が上昇しており、AlとFeの影響は類似した。他の元素においても特徴的な結果は複数認められ、部位間による傾向の違いも見られた。来年度はこれらについても解析を進めたい。それぞれの抽出液における分子量別元素プロファイリングについても検討する。
次に、窒素、リン、カリウムのいずれかを欠乏させた圃場および全て施肥した圃場で栽培した異なる4植物種の根圏および非根圏土壌における抽出性元素濃度の差異を水抽出と0.1 M塩酸抽出でそれぞれ調べた。その結果、水抽出と塩酸抽出では多くの元素で傾向が異なり、根圏において同様の挙動を示す元素グループが複数確認された。種間においても傾向の違いは見られ、それぞれの養分元素に対する植物側の元素集積プロファイルとの比較から植物根の元素可給化能の違いを説明できる可能性が示された。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20K05762

How does "Coelacanth" in plant kingdom cope under hyper-stress environments?
Grants-in-Aid for Scientific Research
30 Jul. 2020 - 31 Mar. 2022
WASAKI JUN
“Solfatara field” located around volcanic vents is a typical acid sulfate soil, which is the severe environment for plants by the hyper-stresses, such as very low pH, Al toxicity, starvation of essential elements, and inhibition of respiration by hydrogen sulfide. The aim of this study is to investigate the unique tolerant strategies of a Lycophytes, Lycopodium cernuum, which makes pure plant community in solfatara fields. It was indicated that specific tolerances to low-pH and aluminum of L. cernuum were important to grow under the hyper-stressed environments. It is suggested that isolation of free Al ion in vacuole is one of strategies of Al tolerance. Our results also implies that L. cernuum may utilize Al in intact plants. It was suggested that microbial community structure in the rhizosphere of L. cernuum was distinctive in solfatara fields, although involvement of the endophytes in the tolerance of L. cernuum is still unclear.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hiroshima University, 20K21315

Application of trace element solubilizing mechanism in rhizosphere and regulate radioactive cesium uptake
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
01 Apr. 2019 - 31 Mar. 2022
SHINANO TAKURO
The short-lived radiocarbon, 11C, is generated as carbon dioxide gas by a cyclotron, collected, and immediately assimilated into the lupin of a legume plant, and a system was constructed to observe the subsequent transfer of 11C to the roots and secretion from the roots to the surrounding soil. The results of the study are shown in Table 1. PET was used for detection. The use of lupin revealed that large amounts of assimilates were secreted from specific root sites (cluster root) into the rhizosphere soil, but not similarly in all cluster roots, and only in certain cluster roots.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 19H01169

New foresight on root exudation: utilization for agricultural production and ecological function
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2017 - 31 Mar. 2020
Wasaki Jun
This study aimed (1) to clarify the formation of cluster roots and their function and (2) to survey the possibility to utilize on crop production and their ecological functions using cluster root-forming plants, which have super-tolerant to low-P environments. It was suggested that the sparingly soluble organic P fraction in the rhizosphere soil was mobilized by the cluster roots. It was also suggested that the nutrient accumulation pattern of neighbor plants of cluster root-forming plants was altered by rhizosphere sharing with cluster roots. P accumulation of main crop intercropped with cluster root-forming plants was higher than monocropped plants. This result suggests a possibility to decrease of usage of P fertilizer by intercropping of cluster root-forming plants.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hiroshima University, 17H03783

樹皮組織のイオノミクス
科学研究費補助金(挑戦的萌芽研究)
2016 - 2018
佐野雄三
文部科学省, Competitive research funding

膜脂質層の分子的改変による、イオンストレスマルチ耐性植物の創生
科学研究費補助金(基盤研究(B))
2015 - 2017
我妻忠雄
文部科学省, Competitive research funding

Why toxic elements can be beneficial in some plant species
Grants-in-Aid for Scientific Research
2015 - 2017
Watanabe Toshihiro
In the present study, as one of the mechanisms responsible for the "beneficial effects" of toxic elements on plant growth, we expected the involvement of the activation of antioxidative stress responses induced by these toxic elements. We focused on aluminum and sodium, which are well known as toxic and beneficial elements. No direct result supporting this hypothesis was observed for aluminum in experiments using Melastoma. For sodium, by contrast, the hypothesis was supported in sugar beet under light-induced oxidative stress. However, chlorophyll fluorescence analysis suggested no involvement of photosynthesis in this phenomenon. Further studies such as measurement of antioxidants seems necessary.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 15K14673

New development of mechanisms of aluminum accumulation and aluminum tolerance in aluminum accumulator woody plants
Grants-in-Aid for Scientific Research
2015 - 2017
Watanabe Toshihiro
In this study, we comprehensively analyzed how Al-accumulator woody plants adapt to aluminum (Al) stress by analyzing gene expression, element, metabolite, and Al-form mainly using Melastoma malabathricum (Melastoma). As a result, the whole aspect of gene expression related to Al absorption, transportation and accumulation in Melastoma, the importance of Al-citrate chelate in Al accumulation in leaves, and the importance of phenolics in Al accumulation of woody plants were clarified. The results obtained in this study will give many suggestions in future research on mechanisms of adaptation to excess Al / mechanisms of Al accumulation, in Al-accumulator woody plants.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 15H04465

The study of the mechanism of green stem disorder insensitivity of soybean RILs using metabolomics and ionomics techniques
Grants-in-Aid for Scientific Research
01 Apr. 2013 - 31 Mar. 2016
Nakamura Takuji, Okazaki Keiki, Yamada Tetsuya, Shinano Takuro, Fukusaki Eichiro, Watanabe Toshihiro
Green stem disorder (GSD) causes a serious problem in the soybean harvesting because seed coat surfaces are soiled and its quality is deteriorated during machine harvesting. The metabolite- and element-compositions in the stems of soybean RILs with different GSD sensitivity were examined using the metabolomics and ionomics technique to evaluate the relationship between the genetical traits and physiological mechanism of GSD insensitivity. In the sensitive RIL to GSD, the amino acid and organic acid metabolism were activated, results in the increase of nitrogen content of stems. It was suggested GSD was activated the nitrogen metabolism because the increase of the amount of translocation of the element to the stems, not to seeds.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), National Agriculture and Food Research Organization, 25450089

開放系オゾン付加施設で生育する冷温帯樹種の成長に及ぼす窒素沈着の影響解明と応用
科学研究費補助金(基盤研究(B))
2014 - 2016
小池孝良
文部科学省, Competitive research funding

尻腐れ果の発生機構の解明と克服のための統合的アプローチ
科学研究費補助金(基盤研究(A))
2014 - 2015
金山喜則
文部科学省, Competitive research funding

イオノミクスを活用した果実の栄養強化に関する研究
科学研究費補助金(挑戦的萌芽研究)
2014 - 2015
金山喜則
文部科学省, Competitive research funding

Variation of mineral accumulation and acquisition in plant
Grants-in-Aid for Scientific Research
2012 - 2014
Watanabe Toshihiro
In the present study, the diversity of element accumulation was widely investigated in terms of variation by genetic factors, chemical forms and translocation in plant, and soil-plant interaction. Variation in foliar concentration among different species (approximately 850 species) tended to be small in essential elements but large in nonessential elements. Variations in the chemical form of aluminum in leaves of various aluminum accumulators was small, and the common form of Al was detected. In leaves of soybean, concentrations of phosphorus, potassium, and cupper decreased and those of many other elements, particularly molybdenum, increased with aging. The correlations of element concentrations between leaf and soil (available concentration) were determined in each element and in each species. Only some elements showed positive correlations under phylogenetic influence.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 24580088

Making of multi-tolerant plants against complexed acid-soil stresses by molecular modifications of membrane lipid layers
Grants-in-Aid for Scientific Research(基盤研究(B))
2011 - 2013
Tadao WAGATSUMA, Keitarou TAWARAYA, Jun WASAKI, Toshihiro WATANABE, Hiroyuki KOYAMA, Tomonobu TOYOMASU, Masaharu KURODA, Akira OIKAWA
In order to lower the content of phospholipids and to increase that of sterols in roots, we made the new plants by modification using two different techniques, i.e., culturing method and molecular method: Al-sensitive rice cultivar Koshihikari grown previously under P-deficient conditions and transformed by the overexpressions of PAH (key gene to exchange phospholipids into galactolipids) and HMG (key gene to biosynthesize sterols). Al tolerance and low-Ca tolerance under low pH conditions of P-deficient Al-sensitive Koshihikari were significantly enhanced, and Al tolerance and low-P tolerance of the several lines of the transformed Koshihikari were also enhanced. Among several tens of rice cultivars, low-P tolerant rice cultivar was found out to have a specific characteristics on the decomposition of P-containing metabolites. Several genes contributing to P-recycling ability were identified together with each function.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 山形大学, Coinvestigator not use grants, Competitive research funding, 23380041

Ionomic response of plant to environmental stress
Grants-in-Aid for Scientific Research(若手研究(B))
2010 - 2011
Toshihiro WATANABE
Effects of nutrient deficiency and aluminum toxicity stresses on plant ionome, all the mineral nutrient and trace elements found in an organism, were investigated. Nitrogen, phosphorus, and potassium deficiencies greatly changed ionome profile in plant. Among analyzed elements, changes in concentration of molybdenum and cesium were noticeable. It was expected that increase in molybdenum concentration under nitrogen deficient conditions could be related to nitrogen-fixing microorganisms. Aluminum stress also affected ionome profile in plant
Ministry of Education, Culture, Sports, Science and Technology, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 22780053

Cesium accumulation in plant　　　　　　　　　　　　　　　
2010
Competitive research funding

イオノームによるミヤコグサの養分動態変異体の獲得と解析
科学研究費補助金(特定領域研究)
2008 - 2009
信濃 卓郎, 渡部 敏裕
マメ科モデル植物のミヤコグサ(Lotus japonicus)の養分吸収変異体を獲得するために、これまでに確立した吸収元素の網羅的解析手法であるイオノーム手法を用いた。変異体のスクリーニング対象としてはEMS(ethylmethane sulfonate)処理を行った種子を自分たちで調整した約200系統およびミヤコグサバイオリソースセンターから提供された約7000糸統を用いた。スクリーニング対象とした元素は必須元素としてはB, Mg, K, Ca, Mn, Fe, Cu, Zn, Mo、非必須元素としてはAI, As, Na, Ni, Sr, Cd, Csであり、非必須元素は野生株の生育に障害が出ない程度のレベルで培養液に添加して栽培を行なった。栽培は一定の環境条件下で同時に200系統を栽培し、全ての個体の地上部を別々に分解をし、対象元素含有率の測定をICP-MSを用いて行った。 200系統全体を母集団として、その平均から3倍以上あるいは半分以下にいずれかの元素含有率が変動した場合に変異を生じている候補として選抜し、地上部を切除した後の根部から植物体を再生することで種子を獲得した。同様の操作を繰り返し4世代目にいたっても元素含有率に変動か生じることを確認した系統を変異系統とした。 2000系統から32の変異系統が獲得された。得られた変異体の中で一つの元素だけが変動している系統は少なく、その平均は4-5種類の元素が同時に変異していることが示された。また元素の種類によっては変動株が多く得られるものがあり、その逆も認められた。実際に獲得したMoの変異株は体内めMoトランスポーターに変異が生じていることが確認され、本手法の有効性が認められた。また、化学的性質の異なる元素が体内においで一定のバランスを持っで存在するイオンホメオスタシスの現象が認められ、変異によってこのバランスかくずれる可能性が初めて示唆された。
文部科学省, 特定領域研究, 北海道大学->独立行政法人農業技術研究機構->独立行政法人農業・食品産業技術総合研究機構, Coinvestigator not use grants, Competitive research funding, 20053001

体内イオンバランスの調整に関与する遺伝子のイオノームによる解析手法の開発
科学研究費補助金(特定領域研究)
2006 - 2007
信濃 卓郎, 渡部 敏裕
誘導結合プラズマ質量分析(ICP-MS)法を用いてマメ科のモデル植物であるミヤコグサの各種有害元素(アルミニウム、ヒ素、カドミウム、セシウム、鉛、モリブデン、ニッケル、塩、ストロンチウム)の吸収、利用機構についての網羅的解析を進めた。昨年度までに各種有害元素の施与条件、分析条件等の確立を行ない、これまでに変異剤(EMS)処理を行なった約2000株の養分吸収能に関わる変異のスクリーニングを進めた。有害元素を含む20種類の異なる元素に関して植物体全体での濃度に変異が認められる系統、特定の部位(根あるいは地上部)に局在する系統を中心に植物と種子の獲得を行なっている。その結果、銅,マンガン,マグネシウム,モリブデン,カドミウム,ニッケル,ヒ素に関して通常の野生株とは全くことなる挙動を示す変異株が存在することが明らかとなった。このように、当初の目的であるイオノームによる解析手法の開発はほぼ完了したと判断される。そこで、特に挙動の異なるマグネシウム変異体に関して戻し交雑をすすめることにより、変異を引き起こしている遺伝子の獲得を進めている。このマグネシウム変異体は地上部へのマグネシウムの移行が抑制されており、生育量に大きな違いは出ないにも関わらず葉脈間に黄化が認められるという表現系を伴うため、変異の発現の確認が用意である利点もある。ミヤコグサはマメ科のモデル植物であり、マメ科はイネ科植物と比較してカルシウム、マグネシウムの吸収量が多いことが知られており、同じ二価のカチオンであるマグネシウムの吸収移行変異体の獲得は今後のマメ科作物の養分吸収機構を解析するための極めて重要な情報を提供することが期待される。
文部科学省, 特定領域研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 18056001

重金属集積植物の探査とその機能解析
科学研究費補助金(若手研究(B))
2004 - 2006
渡部 敏裕
1)昨年度までの実験で、インディアンマスタードとアマランサスは水耕条件では地上部に同程度カドミウムを集積するが、土耕条件ではインディアンマスタードの集積能力はアマランサスと比べて著しく劣ることがわかった。本年度の研究から、この土耕における異なる集積能力は根から分泌される主要な有機酸の種類が関与していることが示唆された。すなわち、アマランサスは不溶性カドミウムを可溶化する能力の高いクエン酸を分泌する一方、インディアンマスタードは可溶化能の低いシュウ酸が分泌性有機酸の主体であった。インディアンマスタード、アマランサスともにカドミウム以外の重金属も超集積する傾向があり、様々な重金属に汚染された土壌のファイトレメディエーションに利用可能であることが示唆された。2)昨年度までの実験で、カドミウム汚染土壌への零価鉄添加が植物のカドミウム集積低減に有効である事がわかった。本年度はその低減メカニズムを土壌化学的手法により解明した。カドミウムで汚染した水田土壌に零価鉄を添加することにより、土壌中のカドミウムは、植物に吸収されやすい交換態などの形態が減少し、吸収されにくい吸蔵態画分が増加することが明かとなった。3)鉱山跡地に生育する野生植物の根圏より採取した土壌から、カドミウム耐性細菌を分離した。分離した細菌をイネ幼植物体の根に塗布し、カドミウム汚染土壌で無菌栽培したところ、塗布しなかったコントロールと比較して大きく生育が改善された。
文部科学省, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 16780043

Investigation and application of adaptation strategies of plant roots to acid soil.
Grants-in-Aid for Scientific Research(基盤研究(A))
2004 - 2006
Mitsuru OSAKI, Takuro SHINANO, Toshihiro WATANABE, Hiroyuki KOYAMA, Keitaro TAWARAYA, Jun WASAKI
This study aimed to solve problems on acid soils for utilization as arable soils. Critical problems of acid soil are 1) toxicity of metallic ions and 2) phosphorus starvation. Some plant species have been developed some strategies to adapt on acid soils. We have shown that the several root exudates bring the tolerance to stresses on acid soils. Therefore, the secreting abilities of plant roots were investigated in this study.A gene for secretory acid phosphatase isolated from white lupin, which is well known as a phosphorus tolerative plant, was introduced into tobacco plants. The growth and phosphorus acquisition of transgenic plant were improved from wild type plants. To investigate the secreting ability of organic acid, which can liberate inorganic phosphate from sparingly soluble forms, malate exudation of Arabidopsis was chosen as a model. It was indicated that malate release was induced by the up-regulation of malate transporter protein. The induction of malate transporter expression by aluminum was specific in the root tips and internal tissues of roots, suggesting that the mechanism is regulated to minimize the carbon consumption. It is concluded that site-specific regulation of the transporters is required to improve the inorganic phosphate uptake ability.It was suggested that the specificities of mucilage, consisted of polysaccharides, in the root exudates differed among plant species. The highest affinities of mucilage of Melastoma malabathricum and maize were shown with trivalent and divalent cations, respectively. The affinity of mucilage of Melastoma malabathricum was high to aluminum ion, though the binding strength was quite lower than that of maize. It was supposed that not only the composition of sugar but also methylation of uronic acids were involved in the differences of adsorption specificity to the cation. Separation and purification of substances involved in the elongation of mycorrhizal hyphae was conducted. It was succeeded that an A2 fraction, which induced an elongation and secondary and further branching of mycorrhizal hyphae was obtained from root exudates of onion.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(A), 北海道大学, Coinvestigator not use grants, Competitive research funding, 16208008

Ionomics in plant and soil　　　　　　　　　　　　　　　
2003
Competitive research funding

Acid soil tolerance in plants　　　　　　　　　　　　　　　
1992
Competitive research funding

セシウム吸着材、その利用、および、フィチン酸鉄塩の製造方法　　　　　　　　　　　　　　　
Patent right, 海野 佑介, 信濃 卓郎, 渡部 敏裕, 高雄 惇英
特願2015-115268, 05 Jun. 2015