Sep. 2019, The Akiyama Life Science Foundation, The Akiyama Life Science Foundation Award 2019　　　　　　　　　　　　　　　
腸内細菌叢の制御における胆汁酸の役割に関する微生物生理学的研究
YOKOTA Atsushi

Jul. 2019, Japan Society for Lactic Acid Bacteria, Japan Society for Lactic Acid Bacteria (JSLAB) Award　　　　　　　　　　　　　　　
Comprehensive studies on the interactions between bile acid and lactic acid bacteria/bifidobactria/gut microbes
YOKOTA Atsushi

Oct. 2012, The Society for Biotechnology, Japan, Achievement Award of the Society for Biotechnology, Japan　　　　　　　　　　　　　　　
Basic research on the enhancement of central metabolism in bacteria producing useful metabolites
YOKOTA Atsushi

Mar. 1996, The Japan Society for Bioscience, Biotechnology, and Agrochemistry, The Japan Society for Bioscience, Biotechnology, and Agrochemistry Award for Encouragement of Young Scientists　　　　　　　　　　　　　　　
エネルギー代謝変異による有用微生物の育種に関する研究
YOKOTA Atsushi

Bile acid is a responsible host factor for high-fat diet-induced gut microbiota alterations in rats: proof of the "bile acid hypothesis".
Masamichi Watanabe, Yuki Fujita, Masahito Hagio, Satoshi Ishizuka, Yoshitoshi Ogura, Tetsuya Hayashi, Satoru Fukiya, Atsushi Yokota
Bioscience of microbiota, food and health, 44, 2, 110, 121, 2025, [Domestic magazines]
English, Scientific journal, High-fat diet (HFD)-induced alterations in gut microbiota may be associated with host pathophysiology, prompting increased interest in elucidating their causal relationships. However, the mechanisms by which HFDs induce these alterations require further clarification. Our previous study using cholic acid (CA)-fed rats suggested that bile acid drives the HFD-induced microbiota alterations as a host factor, a concept termed the "bile acid hypothesis". We analyzed the alterations in the cecal microbiota and bile acid composition in HFD-fed rats and compared the results with those of rats on a CA-supplemented diet. In both cases, the concentrations of total bile acids, including highly bactericidal deoxycholic acid (DCA), increased, concomitant with the increases in the Firmicutes (Bacillota)/Bacteroidetes (Bacteroidota) ratio. Operational taxonomic units (OTUs), accounting for 63.39% of the cecal microbiota of control rats, showed a significant correlation with the total bile acid concentration in HFD-fed rats. A DCA sensitivity test conducted in Firmicutes isolates, corresponding to the predominant OTUs from the HFD-fed rats, exhibited significantly higher DCA resistance compared with Bacteroidetes. The top 12 most abundant OTUs of Firmicutes and Bacteroidetes showing positive or negative correlations with the total bile acid concentration were selected from the HFD-fed rats, and their dynamics were compared with those in the CA-fed rats. Of the 24 OTUs, 18, which constituted 48.28% of the cecal population in the control rats, were altered in the same direction (increase or decrease) in the HFD- and CA-supplemented diet groups. Therefore, approximately half of the cecal populations in the control rats were affected by bile acids, substantiating the bile acid hypothesis microbiologically and quantitatively.

Improved fermentative gamma-aminobutyric acid production from glucose by the inactivation of respiratory chain components NDH-I and Cytbo₃ in Escherichia coli.
Hiroki Wakahara, Takuya Mizokoshi, Kotaro Yamagami, Satoru Fukiya, Atsushi Yokota, Tomoya Maeda
Journal of bioscience and bioengineering, 07 Sep. 2024, [Domestic magazines]
English, Scientific journal, Gamma-aminobutyric acid (GABA), which is synthesized from l-glutamic acid via glutamate decarboxylase (Gad), is used as food, supplements, and biodegradable plastics. Our previous study demonstrated an Escherichia coli mutant (ΔΔ) strain, lacking type I NADH dehydrogenase (NDH-I) and cytochrome bo3 oxidase (Cytbo3), produced 7 g/L glutamic acid on MS1 glucose-minimal medium. In this study, the ΔΔ strain was used for improving GABA production. A plasmid (pMBL19-gadB') expressing a mutated E. coli GadB (Glu89Gln/Δ452-466), retaining activity at neutral pH, was introduced into the ΔΔ strain and its parent strain (W1485). The ΔΔ strain carrying pMBL19-gadB' exhibited a twofold increase in GABA production compared to the W1485 strain carrying pMBL19-gadB'. Deleting the C-terminal (Δ471-511) of GadC antiporter in the ΔΔ strain further improved GABA yield by 1.5 g/L when cultured in MS1 glucose-minimal medium. On the other hand, a large amount of glutamic acid produced by the ΔΔ strain was not fully converted to GABA, likely due to the inhibition of GadB activity by the accumulation of acetic acid. Although there is room for improvement, these results indicate the efficacy of the ΔNDH-IΔCytbo3 double mutation in augmenting GABA production.

Contributions of the anaplerotic reaction enzymes pyruvate carboxylase and phosphoenolpyruvate carboxylase to l-lysine production in Corynebacterium glutamicum.
Akinobu Shinmori, Zhen Guo, Tomoya Maeda, Satoru Fukiya, Masaru Wada, Atsushi Yokota
Journal of bioscience and bioengineering, 27 Jun. 2024, [Domestic magazines]
English, Scientific journal, Anaplerotic reactions catalyzed by pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPC) have important roles in the production of l-lysine to replenish oxaloacetic acid (OAA) in Corynebacterium glutamicum. However, the relative contributions of these enzymes to l-lysine production in C. glutamicum are not fully understood. In this study, using a parent strain (P) carrying a feedback inhibition-resistant aspartokinase with the T311I mutation, we constructed a PC gene-deleted mutant strain (PΔPC) and a PEPC gene-deleted mutant strain (PΔPEPC). Although the growth of both mutant strains was comparable to the growth of strain P, the maximum l-lysine production in strains PΔPC and PΔPEPC decreased by 14% and 49%, respectively, indicating that PEPC strongly contributed to OAA supply. l-Lysine production in strain PΔPC slightly decreased during the logarithmic phase, while production during the early stationary phase was comparable to production in strain P. By contrast, strain PΔPEPC produced l-lysine in an amount comparable to the production of strain P during the logarithmic phase; l-lysine production after the early stationary phase was completely stopped in strain PΔPEPC. These results indicate that OAA is supplied by both PC and PEPC during the logarithmic phase, while only PEPC can continuously supply OAA after the logarithmic phase.

Synthesis and quantification of short-chain fatty acid esters of hydroxy fatty acids in rat intestinal contents and fecal samples by LC-MS/MS
Siddabasave Gowda B. Gowda, Fengjue Hou, Divyavani Gowda, Hitoshi Chiba, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Shu-Ping Hui
Analytica Chimica Acta, 1288, 342145, 342145, Elsevier BV, Feb. 2024
Scientific journal

Development of an improved colonization system for human-derived Bifidobacterium longum subsp. longum in conventional mice through the feeding of raffinose or 1-kestose.
Mina Shimada, Youhei Kawase, Kei Sonoyama, Yoshitoshi Ogura, Tetsuya Hayashi, Atsushi Yokota, Satoru Fukiya
Bioscience of microbiota, food and health, 43, 2, 110, 119, 2024, [Domestic magazines]
English, Scientific journal, How bifidobacteria colonize and survive in the intestine is not fully understood. The administration of bifidobacteria to conventional mice can be used to evaluate their ability to colonize the intestine in the presence of endogenous gut microbiota. However, human-derived bifidobacteria do not readily colonize the intestines of conventional mice, and although colonization by Bifidobacterium breve UCC2003 has been achieved, the viability of such populations requires improvement. Therefore, we aimed to establish a colonization system with human-derived bifidobacteria of high viability in conventional mice using Bifidobacterium longum subsp. longum 105-A. Lactose, raffinose, and 1-kestose were identified as the preferred carbohydrate sources for the growth of this strain in culture. The administration of B. longum 105-A to conventional BALB/c mice fed these carbohydrates showed that diets containing 6% (w/w) raffinose or 1-kestose facilitated colonization with >108 colony-forming units/g feces for 2 weeks. The population of this strain was more stable in the raffinose-fed group than in the 1-kestose-fed group. The ingestion of these prebiotics had a greater impact on the composition of the microbiota than the administration of B. longum 105-A. The ingestion of these prebiotics also increased the fecal concentrations of organic acids, which was indicative of greater intestinal fermentation. Collectively, we established a colonization system for B. longum 105-A with high viability in conventional mice by feeding the mice raffinose or 1-kestose. This system should be useful for elucidation of the mechanisms of colonization and survival of bifidobacteria in the intestines in the presence of the endogenous gut microbiota.

Bifidobacterium longum subsp.longumにおける1-Kestose代謝メカニズムの解明　　　　　　　　　　　　　　　
難波 有希, 倉持 碧海, 山口 颯人, 吉田 佳鼓, 後藤 恭宏, 門田 吉弘, 栃尾 巧, 小椋 義俊, 林 哲也, 前田 智也, 横田 篤, 吹谷 智
日本乳酸菌学会誌, 33, 2, 120, 120, 日本乳酸菌学会, Jun. 2022
Japanese

腸内での二次胆汁酸生成と腸内細菌叢変化・疾病の誘導　　　　　　　　　　　　　　　
吹谷 智, 横田 篤, 石塚 敏
機能性食品と薬理栄養, 15, 4, 229, 234, (株)インフォノーツパブリッシング, Feb. 2022, [Invited]
Japanese, Scientific journal

Diversification of a Fucosyllactose Transporter within the Genus Bifidobacterium.
Miriam N Ojima, Yuya Asao, Aruto Nakajima, Toshihiko Katoh, Motomitsu Kitaoka, Aina Gotoh, Junko Hirose, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota, Maher Abou Hachem, Mikiyasu Sakanaka, Takane Katayama
Applied and environmental microbiology, 88, 2, e0143721, 25 Jan. 2022, [International Magazine]
English, Scientific journal, Human milk oligosaccharides (HMOs), which are natural bifidogenic prebiotics, were recently commercialized to fortify formula milk. However, HMO assimilation phenotypes of bifidobacteria vary by species and strain, which has not been fully linked to strain genotype. We have recently shown that specialized uptake systems, particularly for the internalization of major HMOs (fucosyllactose [FL]), are associated with the formation of a Bifidobacterium-rich gut microbial community. Phylogenetic analysis revealed that FL transporters have diversified into two clades harboring four clusters within the Bifidobacterium genus, but the underpinning functional diversity associated with this divergence remains underexplored. In this study, we examined the HMO consumption phenotypes of two bifidobacterial species, Bifidobacterium catenulatum subsp. kashiwanohense and Bifidobacterium pseudocatenulatum, both of which possess FL-binding proteins that belong to phylogenetic clusters with unknown specificities. Growth assays, heterologous gene expression experiments, and HMO consumption analyses showed that the FL transporter type from B. catenulatum subsp. kashiwanohense JCM 15439T conferred a novel HMO uptake pattern that includes complex fucosylated HMOs (lacto-N-fucopentaose II and lacto-N-difucohexaose I/II). Further genomic landscape analyses of FL transporter-positive bifidobacterial strains revealed that the H-antigen- or Lewis antigen-specific fucosidase gene(s) and FL transporter specificities were largely aligned. These results suggest that bifidobacteria have acquired FL transporters along with the corresponding gene sets necessary to utilize the imported HMOs. Our results provide insight into the species- and strain-dependent adaptation strategies of bifidobacteria in HMO-rich environments. IMPORTANCE The gut of breastfed infants is generally dominated by health-promoting bifidobacteria. Human milk oligosaccharides (HMOs) from breast milk selectively promote the growth of specific taxa such as bifidobacteria, thus forming an HMO-mediated host-microbe symbiosis. While the coevolution of humans and bifidobacteria has been proposed, the underpinning adaptive strategies employed by bifidobacteria require further research. Here, we analyzed the divergence of the critical fucosyllactose (FL) HMO transporter within Bifidobacterium. We have shown that the diversification of the solute-binding proteins of the FL transporter led to uptake specificities of fucosylated sugars ranging from simple trisaccharides to complex hexasaccharides. This transporter and the congruent acquisition of the necessary intracellular enzymes allow bifidobacteria to consume different types of HMOs in a predictable and strain-dependent manner. These findings explain the adaptation and proliferation of bifidobacteria in the competitive and HMO-rich infant gut environment and enable accurate specificity annotation of transporters from metagenomic data.

Comparative Genomic and Physiological Analysis against Clostridium scindens Reveals Eubacterium sp. c-25 as an Atypical Deoxycholic Acid Producer of the Human Gut Microbiota.
Isaiah Song, Yasuhiro Gotoh, Yoshitoshi Ogura, Tetsuya Hayashi, Satoru Fukiya, Atsushi Yokota
Microorganisms, 9, 11, 29 Oct. 2021, [International Magazine]
English, Scientific journal, The human gut houses bile acid 7α-dehydroxylating bacteria that produce secondary bile acids such as deoxycholic acid (DCA) from host-derived bile acids through enzymes encoded by the bai operon. While recent metagenomic studies suggest that these bacteria are highly diverse and abundant, very few DCA producers have been identified. Here, we investigated the physiology and determined the complete genome sequence of Eubacterium sp. c-25, a DCA producer that was isolated from human feces in the 1980s. Culture experiments showed a preference for neutral to slightly alkaline pH in both growth and DCA production. Genomic analyses revealed that c-25 is phylogenetically distinct from known DCA producers and possesses a multi-cluster arrangement of predicted bile-acid inducible (bai) genes that is considerably different from the typical bai operon structure. This arrangement is also found in other intestinal bacterial species, possibly indicative of unconfirmed 7α-dehydroxylation capabilities. Functionality of the predicted bai genes was supported by the induced expression of baiB, baiCD, and baiH in the presence of cholic acid substrate. Taken together, Eubacterium sp. c-25 is an atypical DCA producer with a novel bai gene cluster structure that may represent an unexplored genotype of DCA producers in the human gut.

Bifidobacterium longum subsp.longumにおける1-Kestose代謝メカニズムの解明　　　　　　　　　　　　　　　
難波 有希, 山口 颯人, 吉田 佳鼓, 後藤 恭宏, 門田 吉弘, 栃尾 巧, 小椋 義俊, 林 哲也, 横田 篤, 吹谷 智
日本生物工学会大会講演要旨集, 2021年, 230, 230, (公社)日本生物工学会, Oct. 2021
Japanese

Dietary raffinose ameliorates hepatic lipid accumulation induced by cholic acid via modulation of enterohepatic bile acid circulation in rats.
Kenta Maegawa, Haruka Koyama, Satoru Fukiya, Atsushi Yokota, Koichiro Ueda, Satoshi Ishizuka
The British journal of nutrition, 127, 11, 1, 10, 14 Jul. 2021, [International Magazine]
English, Scientific journal, Enterohepatic circulation of 12α-hydroxylated (12αOH) bile acid (BA) is enhanced depending on the energy intake in high-fat diet-fed rats. Such BA metabolism can be reproduced using a diet supplemented with cholic acid (CA), which also induces simple steatosis, without inflammation and fibrosis, accompanied by some other symptoms that are frequently observed in the condition of non-alcoholic fatty liver in rats. We investigated whether supplementation of the diet with raffinose (Raf) improves hepatic lipid accumulation induced by the CA-fed condition in rats. After acclimation to the AIN-93-based control diet, male Wistar rats were fed diets supplemented with a combination of Raf (30 g/kg diet) and/or CA (0·5 g/kg diet) for 4 weeks. Dietary Raf normalised hepatic TAG levels (two-way ANOVA P < 0·001 for CA, P = 0·02 for Raf and P = 0·004 for interaction) in the CA-supplemented diet-fed rats. Dietary Raf supplementation reduced hepatic 12αOH BA concentration (two-way ANOVA P < 0·001 for CA, P = 0·003 for Raf and P = 0·03 for interaction). The concentration of 12αOH BA was reduced in the aortic and portal plasma. Raf supplementation increased acetic acid concentration in the caecal contents (two-way ANOVA P = 0·001 as a main effect). Multiple regression analysis revealed that concentrations of aortic 12αOH BA and caecal acetic acid could serve as predictors of hepatic TAG concentration (R2 = 0·55, P < 0·001). However, Raf did not decrease the secondary 12αOH BA concentration in the caecal contents as well as the transaminase activity in the CA diet-fed rats. These results imply that dietary Raf normalises hepatic lipid accumulation via suppression of enterohepatic 12αOH BA circulation.

Primary 12α-Hydroxylated Bile Acids Lower Hepatic Iron Concentration in Rats.
Shota Hori, Minako Satake, Ohji Kohmoto, Ryo Takagi, Kazufumi Okada, Satoru Fukiya, Atsushi Yokota, Satoshi Ishizuka
The Journal of nutrition, 151, 3, 523, 530, 11 Mar. 2021, [International Magazine]
English, Scientific journal, BACKGROUND: Primary 12α-hydroxylated bile acids (12αOH BAs) enhance intestinal iron uptake due to their ability ex vivo to chelate iron. However, no information is available on their role in vivo, especially in the liver. OBJECTIVES: To investigate the effects and mechanisms of primary 12αOH BAs on hepatic iron concentration in vivo. METHODS: Male Wistar King A Hokkaido male rats (WKAH/HkmSlc) rats aged 4-5 weeks were fed a control diet or a diet with cholic acid (CA; 0.5 g/kg diet), the primary 12αOH BA, for 2 weeks (Study 1) or 13 weeks (Study 2). In Study 3, rats fed the same diets were given drinking water either alone or containing vancomycin (200 mg/L) for 6 weeks. The variables measured included food intake (Studies 1-3), bile acid profiles (Studies 1 and 3), hepatic iron concentration (Studies 1-3), fecal iron excretion (Studies 1 and 2), iron-related liver gene expression (Studies 2 and 3), and plasma iron-related factors (Studies 2 and 3). RESULTS: In Study 1, CA feed reduced the hepatic iron concentration (-16%; P = 0.005) without changing food intake or fecal iron excretion. In Study 2, we found a significant increase in the aortic plasma concentration of lipocalin 2 (LCN2; +65%; P < 0.001), an iron-trafficking protein. In Study 3, we observed no effect of vancomycin treatment on the CA-induced reduction of hepatic iron concentration (-32%; P < 0.001), accompanied by increased plasma LCN2 concentration (+72%; P = 0.003), in the CA-fed rats despite a drastic reduction in the secondary 12αOH BA concentration (-94%; P < 0.001) in the aortic plasma. CONCLUSIONS: Primary 12αOH BAs reduced the hepatic iron concentration in rats. LCN2 may be responsible for the hepatic iron-lowering effect of primary 12αOH BAs by transporting iron out of the liver.

Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile
Rika Hirano, Mikiyasu Sakanaka, Kazuto Yoshimi, Naohisa Sugimoto, Syogo Eguchi, Yuko Yamauchi, Misaki Nara, Shingo Maeda, Yuta Ami, Aina Gotoh, Takane Katayama, Noriho Iida, Tamotsu Kato, Hiroshi Ohno, Satoru Fukiya, Atsushi Yokota, Mamoru Nishimoto, Motomitsu Kitaoka, Hiroyuki Nakai, Shin Kurihara
Gut Microbes, 13, 1, Informa UK Limited, 01 Jan. 2021
Scientific journal

Methionine utilization by bifidobacteria: possible existence of a reverse transsulfuration pathway.
Masaru Wada, Satoru Fukiya, Azusa Suzuki, Nanae Matsumoto, Miki Matsuo, Atsushi Yokota
Bioscience of microbiota, food and health, 40, 1, 80, 83, 2021, [Domestic magazines]
English, Scientific journal, Although bifidobacteria are already widely used as beneficial microbes with health-promoting effects, their amino acid utilization and metabolism are not yet fully understood. Knowledge about the metabolism of sulfur-containing amino acids in bifidobacteria is especially limited. In this study, we tested the methionine utilization ability of several bifidobacterial strains when it was the sole available sulfur source. Although bifidobacteria have long been predominantly considered to be cysteine auxotrophs, we showed that this is not necessarily the case.

12α-Hydroxylated bile acid induces hepatic steatosis with dysbiosis in rats
Ja Young Lee, Hidehisa Shimizu, Masahito Hagio, Satoru Fukiya, Masamichi Watanabe, Yasutake Tanaka, Ga Hyun Joe, Hitoshi Iwaya, Reika Yoshitsugu, Keidai Kikuchi, Misaki Tsuji, Nanako Baba, Takuma Nose, Koji Tada, Taketo Hanai, Shota Hori, Akari Takeuchi, Yumiko Furukawa, Bungo Shirouchi, Masao Sato, Tadasuke Ooka, Yoshitoshi Ogura, Tetsuya Hayashi, Atsushi Yokota, Satoshi Ishizuka
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1865, 12, Dec. 2020
Scientific journal

Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
Siddabasave Gowda B. Gowda, Divyavani Gowda, Chongsheng Liang, Yonghan Li, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu-Ping Hui
Metabolites, 10, 10, 398, 398, MDPI AG, 08 Oct. 2020
Scientific journal, Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.

Identification of short‐chain fatty acid esters of hydroxy fatty acids (SFAHFAs) in a murine model by nontargeted analysis using ultra‐high‐performance liquid chromatography/linear ion trap quadrupole‐Orbitrap mass spectrometry
Siddabasave Gowda B. Gowda, Chongsheng Liang, Divyavani Gowda, Fengjue Hou, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu‐Ping Hui
Rapid Communications in Mass Spectrometry, 34, 17, Wiley, 15 Sep. 2020
Scientific journal

Association between 12α-hydroxylated bile acids and hepatic steatosis in rats fed a high-fat diet.
Shota Hori, Takayuki Abe, Dong Geun Lee, Satoru Fukiya, Atsushi Yokota, Nao Aso, Bungo Shirouchi, Masao Sato, Satoshi Ishizuka
The Journal of nutritional biochemistry, 83, 108412, 108412, 20 May 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, High-fat (HF) diet induces hepatic steatosis that is a risk factor for noncommunicable diseases such as obesity, type 2 diabetes and cardiovascular disease. Previously, we found that HF feeding in rats increases the excretion of fecal bile acids (BAs), specifically 12α-hydroxylated (12αOH) BAs. Although the liver is the metabolic center in our body, the association between hepatic steatosis and 12αOH BAs in HF-fed rats is unclear. Thus, we investigated extensively BA composition in HF-fed rats and evaluated the association between hepatic steatosis and 12αOH BAs. Acclimated male inbred WKAH/HkmSlc rats were divided into two groups and fed either control or HF diet for 8 weeks. Feeding HF diet increased hepatic triglyceride and total cholesterol concentrations, which correlated positively with 12αOH BAs concentrations but not with non-12αOH BAs in the feces, portal plasma and liver. Accompanied by the increase in 12αOH BAs, the rats fed HF diet showed increased fat absorption and higher mRNA expression of liver Cidea. The enhancement of 12αOH BA secretion may contribute to hepatic steatosis by the promotion of dietary fat absorption and hepatic Cidea mRNA expression. The increase in 12αOH BAs was associated with enhanced liver cholesterol 7α-hydroxylase (Cyp7a1) and sterol 12α-hydroxylase (Cyp8b1) mRNA expression. There was a significant increase in 7α-hydroxycholesterol, a precursor of BAs, in the liver of HF-fed rats. Altogether, these data suggest that the HF diet increases preferentially 12αOH BAs synthesis by utilizing the accumulated hepatic cholesterol and enhancing mRNA expression of Cyp7a1 and Cyp8b1 in the liver.

Application of Recombinase-Based In Vivo Expression Technology to Bifidobacterium longum subsp. longum for Identification of Genes Induced in the Gastrointestinal Tract of Mice.
Hiroka Koguchi, Natsumi Ishigami, Mikiyasu Sakanaka, Kako Yoshida, Sayaka Hiratou, Mina Shimada, Satoru Fukiya, Kei Sonoyama, Atsushi Yokota
Microorganisms, 8, 3, 13 Mar. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bifidobacteria are one of the major components in human gut microbiota and well-known as beneficial microbes. However, clarification of commensal mechanisms of bifidobacteria in the intestines is still ongoing, especially in the presence of the gut microbiota. Here, we applied recombinase-based in vivo expression technology (R-IVET) using the bacteriophage P1 Cre/loxP system to Bifidobacterium longum subsp. longum 105-A (B. longum 105-A) to identify genes that are specifically expressed in the gastrointestinal tract of conventionally raised mice. Oral administration of the genomic DNA library of B. longum 105-A to conventionally raised mice resulted in the identification of 73 in vivo-induced genes. Four out of seven tested genes were verified in vivo-specific induction at least in the cecum by quantitative reverse transcription PCR. Although there is still room for improvement of the system, our findings can contribute to expanding our understanding of the commensal behavior of B. longum in the gut ecosystem.

Untargeted lipidomic analysis of plasma from high fat diet-induced obese rats using UHPLC- linear trap quadrupole -Orbitrap MS.
Siddabasave Gowda B Gowda, Zi-Jun Gao, Zhen Chen, Takayuki Abe, Shota Hori, Satoru Fukiya, Satoshi Ishizuka, Atsushi Yokota, Hitoshi Chiba, Shu-Ping Hui
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 17 Jan. 2020, [Peer-reviewed], [Domestic magazines]
English

Administration of Cholic Acid Inhibits Equol Production from Daidzein in Mice.
Hiroko Yoshioka, Masamichi Watanabe, Fumio Nanba, Toshio Suzuki, Satoru Fukiya, Atsushi Yokota, Toshiya Toda
Journal of nutritional science and vitaminology, 66, 6, 571, 576, 2020, [Domestic magazines]
English, Scientific journal, Equol (Eq) is a metabolite of soy isoflavone daidzein (De) produced by the intestinal microbiota. The clinical effectiveness of soy isoflavone is considered to depend on the individual ability of Eq production. Previous studies have demonstrated that habitual dietary patterns may influence the production of Eq. For example, high Eq producers consumed less fat as a percentage of energy than low Eq producers. However, the inhibitory factors of Eq production are unknown. Recently, it was reported that bile acids induced by high-fat diet consumption may be a host-related factor controlling the composition of the intestinal microbiota. In this study, we investigated the effect of cholic acid (CA) administration, a mimic of the microbiota altered by a high-fat diet, on Eq production in mice. CA administration significantly decreased the levels of the De metabolites Eq, dihydrodaidzein, and O-desmethylangolensin in the serum of mice. However, CA administration did not affect the total molar concentration of De and its metabolites. Moreover, CA administration increased the levels of secondary bile acids, particularly deoxycholic acid (DCA), which has strong antibacterial activity in the cecum contents of mice. Thus, CA administration may increase the levels of DCA, a secondary bile acid, resulting in inhibition of Eq production. These findings may help to reveal the factors inhibiting Eq production and enhance the clinical effectiveness of isoflavone intake.

Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis.
Mikiyasu Sakanaka, Morten Ejby Hansen, Aina Gotoh, Toshihiko Katoh, Keisuke Yoshida, Toshitaka Odamaki, Hiroyuki Yachi, Yuta Sugiyama, Shin Kurihara, Junko Hirose, Tadasu Urashima, Jin-Zhong Xiao, Motomitsu Kitaoka, Satoru Fukiya, Atsushi Yokota, Leila Lo Leggio, Maher Abou Hachem, Takane Katayama
Science advances, 5, 8, eaaw7696, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that human milk oligosaccharides (HMOs) in breast milk promote the formation of a bifidobacteria-rich microbiota in infant guts; however, the underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA and HMO consumption analyses, combined with deposited metagenome data mining, revealed that FL transporter-2 is primarily associated with the bifidobacteria-rich microbiota formation in breast-fed infant guts. Structural analyses of the solute-binding protein (SBP) of FL transporter-2 complexed with 2'-fucosyllactose and 3-fucosyllactose, together with phylogenetic analysis of SBP homologs of both FL transporters, highlight a unique adaptation strategy of Bifidobacterium to HMOs, in which the gain-of-function mutations enable FL transporter-2 to efficiently capture major fucosylated HMOs. Our results provide a molecular insight into HMO-mediated symbiosis and coevolution between bifidobacteria and humans.

The membrane phospholipid cardiolipin plays a pivotal role in bile acid adaptation by Lactobacillus gasseri JCM1131T.
Shinji Kato, Haruhi Tobe, Hiroki Matsubara, Mariko Sawada, Yasuko Sasaki, Satoru Fukiya, Naoki Morita, Atsushi Yokota
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864, 3, 403, 412, Mar. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bile acids exhibit strong antimicrobial activity as natural detergents, and are involved in lipid digestion and absorption. We investigated the mechanism of bile acid adaptation in Lactobacillus gasseri JCM1131T. Exposure to sublethal concentrations of cholic acid (CA), a major bile acid in humans, resulted in development of resistance to otherwise-lethal concentrations of CA by this intestinal lactic acid bacterium. As this adaptation was accompanied by decreased cell-membrane damage, we analyzed the membrane lipid composition of L. gasseri. Although there was no difference in the proportions of glycolipids (~70%) and phospholipids (~20%), adaptation resulted in an increased abundance of long-sugar-chain glycolipids and a 100% increase in cardiolipin (CL) content (to ~50% of phospholipids) at the expense of phosphatidylglycerol (PG). In model vesicles, the resistance of PG vesicles to solubilization by CA increased with increasing CL/PG ratio. Deletion of the two putative CL synthase genes, the products of which are responsible for CL synthesis from PG, decreased the CL content of the mutants, but did not affect their ability to adapt to CA. Exposure to CA restored the CL content of the two single-deletion mutants, likely due to the activities of the remaining CL synthase. In contrast, the CL content of the double-deletion mutant was not restored, and the lipid composition was modified such that PG predominated (~45% of total lipids) at the expense of glycolipids. Therefore, CL plays important roles in bile acid resistance and maintenance of the membrane lipid composition in L. gasseri.

Ingestion of difructose anhydride III partially suppresses the deconjugation and 7α-dehydroxylation of bile acids in rats fed with a cholic acid-supplemented diet.
Lee DG, Hori S, Kohmoto O, Kitta S, Yoshida R, Tanaka Y, Shimizu H, Takahashi K, Nagura T, Uchino H, Fukiya S, Yokota A, Ishizuka S
Bioscience, Biotechnology, and Biochemistry, 83, 7, 1329, 1335, Mar. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Difructose anhydride III (DFAIII) is a prebiotic involved in the reduction of secondary bile acids (BAs). We investigated whether DFAIII modulates BA metabolism, including enterohepatic circulation, in the rats fed with a diet supplemented with cholic acid (CA), one of the 12α-hydroxylated BAs. After acclimation, the rats were fed with a control diet or a diet supplemented with DFAIII. After 2 weeks, each group was further divided into two groups and was fed diet with or without CA supplementation at 0.5 g/kg diet. BA levels were analyzed in aortic and portal plasma, liver, intestinal content, and feces. As a result, DFAIII ingestion reduced the fecal deoxycholic acid level via the partial suppression of deconjugation and 7α-dehydroxylation of BAs following CA supplementation. These results suggest that DFAIII suppresses production of deoxycholic acid in conditions of high concentrations of 12α-hydroxylated BAs in enterohepatic circulation, such as obesity or excess energy intake. Abbreviation: BA: bile acid; BSH: bile salt hydrolase; CA: cholic acid; DCA: deoxycholic acid; DFAIII: difructose anhydride III; MCA: muricholic acid; MS: mass spectrometry; NCDs: non-communicable diseases; LC: liquid chromatography; SCFA: short-chain fatty acid; TCA: taurocholic acid; TCDCA: taurochenodeoxycholic acid; TDCA: taurodeoxycholic acid; TUDCA: tauroursodeoxychlic acid; TαMCA: tauro-α-muricholic acid; TβMCA: tauro-β-muricholic acid; TωMCA: tauro-ω-muricholic acid.

Engineering of Corynebacterium glutamicum as a prototrophic pyruvate-producing strain: Characterization of a ramA-deficient mutant and its application for metabolic engineering.
Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Wada M, Yokota A, Matsushita K
Bioscience, Biotechnology, and Biochemistry, 83, 2, 372, 380, Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, To construct a prototrophic Corynebacterium glutamicum strain that efficiently produces pyruvate from glucose, the effects of inactivating RamA, a global regulator responsible for activating the oxidative tricarboxylic acid (TCA) cycle, on glucose metabolism were investigated. ΔramA showed an increased specific glucose consumption rate, decreased growth, comparable pyruvate production, higher formation of lactate and acetate, and lower accumulation of succinate and 2-oxoglutarate compared to the wild type. A significant decrease in pyruvate dehydrogenase complex activity was observed for ΔramA, indicating reduced carbon flow to the TCA cycle in ΔramA. To create an efficient pyruvate producer, the ramA gene was deleted in a strain lacking the genes involved in all known lactate- and acetate-producing pathways. The resulting mutant produced 161 mM pyruvate from 222 mM glucose, which was significantly higher than that of the parent (89.3 mM; 1.80-fold).

A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS3 Family Insertion Sequence, ISBlo11.
Mikiyasu Sakanaka, Shingo Nakakawaji, Shin Nakajima, Satoru Fukiya, Arisa Abe, Wataru Saburi, Haruhide Mori, Atsushi Yokota
Applied and environmental microbiology, 84, 17, 01 Sep. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combination with next-generation sequencing, it enables exhaustive identification of genes that contribute to microbial fitness. We now describe a transposon mutagenesis system for Bifidobacterium longum subsp. longum 105-A (JCM 31944) based on ISBlo11, a native IS3 family insertion sequence. To build this system, xylose-inducible or constitutive bifidobacterial promoters were tested to drive the expression of full-length or a truncated form at the N terminus of the ISBlo11 transposase. An artificial transposon plasmid, pBFS12, in which ISBlo11 terminal inverted repeats are separated by a 3-bp spacer, was also constructed to mimic the transposition intermediate of IS3 elements. The introduction of this plasmid into a strain expressing transposase resulted in the insertion of the plasmid with an efficiency of >103 CFU/μg DNA. The plasmid targets random 3- to 4-bp sequences, but with a preference for noncoding regions. This mutagenesis system also worked at least in B. longum NCC2705. Characterization of a transposon insertion mutant revealed that a putative α-glucosidase mediates palatinose and trehalose assimilation, demonstrating the suitability of transposon mutagenesis for loss-of-function analysis. We anticipate that this approach will accelerate functional genomic studies of B. longum subsp. longumIMPORTANCE Several hundred species of bacteria colonize the mammalian intestine. However, the genes that enable such bacteria to colonize and thrive in the intestine remain largely unexplored. Transposon mutagenesis, combined with next-generation sequencing, is a promising tool to comprehensively identify these genes but has so far been applied only to a small number of intestinal bacterial species. In this study, a transposon mutagenesis system was established for Bifidobacterium longum subsp. longum, a representative health-promoting Bifidobacterium species. The system enables the identification of genes that promote colonization and survival in the intestine and should help illuminate the physiology of this species.

乳酸桿菌の対数期に膜小胞上に局在するリポテイコ酸　　　　　　　　　　　　　　　
白石 宗, 横田 伸一, 佐藤 耶舞羽, 伊藤 利章, 吹谷 智, 山本 聡, 佐藤 豊孝, 横田 篤
日本乳酸菌学会誌, 29, 2, 116, 116, 日本乳酸菌学会, Jul. 2018
Japanese

Lipoteichoic acids are embedded in cell walls during logarithmic phase, but exposed on membrane vesicles in Lactobacillus gasseri JCM 1131T
T. Shiraishi, S. Yokota, Y. Sato, T. Ito, S. Fukiya, S. Yamamoto, T. Sato, A. Yokota
Beneficial Microbes, 9, 4, 653, 662, Wageningen Academic Publishers, 2018, [Peer-reviewed]
English, Scientific journal

Isolation of six novel 7-oxo- or urso-type secondary bile acid-producing bacteria from rat cecal contents
Sarinya Tawthep, Satoru Fukiya, Ja-Young Lee, Masahito Hagio, Yoshitoshi Ogura, Tetsuya Hayashi, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124, 5, 514, 522, Nov. 2017, [Peer-reviewed]
English, Scientific journal

Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation
Keita Nishiyama, Yuji Yamamoto, Makoto Sugiyama, Takashi Takaki, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota, Nobuhiko Okada, Takao Mukai
MBIO, 8, 5, Sep. 2017, [Peer-reviewed]
English, Scientific journal

Comprehensive evaluation of the bactericidal activities of free bile acids in the large intestine of humans and rodents
Masamichi Watanabe, Satoru Fukiya, Atsushi Yokota
JOURNAL OF LIPID RESEARCH, 58, 6, 1143, 1152, Jun. 2017, [Peer-reviewed]
English, Scientific journal

Lactobacillus gasseri種に共通する種特異的なリポテイコ酸の化学構造　　　　　　　　　　　　　　　
久富 亮佑, 白石 宗, 佐藤 耶舞羽, 森田 直樹, 吹谷 智, 佐藤 豊孝, 横田 篤, 横田 伸一
日本乳酸菌学会誌, 28, 2, 123, 123, 日本乳酸菌学会, Jun. 2017
Japanese

Pyruvate dehydrogenase complex regulator (PdhR) gene deletion boosts glucose metabolism in Escherichia coli under oxygen-limited culture conditions
Soya Maeda, Kumiko Shimizu, Chie Kihira, Yuki Iwabu, Ryuichi Kato, Makoto Sugimoto, Satoru Fukiya, Masaru Wada, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123, 4, 437, 443, Apr. 2017, [Peer-reviewed]
English, Scientific journal

Lactobacillus gasseri種に共通するリポテイコ酸の特異的構造　　　　　　　　　　　　　　　
久富 亮佑, 白石 宗, 佐藤 耶舞羽, 森田 直樹, 吹谷 智, 佐藤 豊孝, 横田 篤, 横田 伸一
日本細菌学雑誌, 72, 1, 86, 86, 日本細菌学会, Feb. 2017
Japanese

Isolation and amino acid sequence of a dehydratase acting on d-erythro-3-hydroxyaspartate from Pseudomonas sp N99, and its application in the production of optically active 3-hydroxyaspartate
Hiroyuki Nagano, Kana Shibano, Yu Matsumoto, Atsushi Yokota, Masaru Wada
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81, 6, 1156, 1164, 2017, [Peer-reviewed]
English, Scientific journal

Boosting Anaplerotic Reactions by Pyruvate Kinase Gene Deletion and Phosphoenolpyruvate Carboxylase Desensitization for Glutamic Acid and Lysine Production in Corynebacterium glutamicum
Atsushi Yokota, Kazunori Sawada, Masaru Wada
AMINO ACID FERMENTATION, 159, 181, 198, 2017, [Peer-reviewed]
English, Scientific journal

Amino Acid Fermentation Preface
Atsushi Yokota, Masato Ikeda
AMINO ACID FERMENTATION, 159, V, VI, 2017, [Peer-reviewed]
English, In book

構造情報の蓄積から見えてきたリポテイコ酸の細菌種による構造多様性.　　　　　　　　　　　　　　　
白石宗, 横田伸一, 吹谷智, 横田篤
日本乳酸菌学会誌, 28, 3, 177, 177, 2017, [Invited]
Japanese, Scientific journal

Pyruvate kinase deletion as an effective phenotype to enhance lysine production in Corynebacterium glutamicum ATCC13032: Redirecting the carbon flow to a precursor metabolite
Masaki Yanase, Tohru Aikoh, Kazunori Sawada, Kotaro Ogura, Takuya Hagiwara, Keita Imai, Masaru Wada, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122, 2, 160, 167, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Effects of phosphoenolpyruvate carboxylase desensitization on glutamic acid production in Corynebacterium glutamicum ATCC 13032
Masaru Wada, Kazunori Sawada, Kotaro Ogura, Yuta Shimono, Takuya Hagiwara, Masakazu Sugimoto, Akiko Onuki, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121, 2, 172, 177, Feb. 2016, [Peer-reviewed]
English, Scientific journal

Structural diversity and biological significance of lipoteichoic acid in Gram-positive bacteria: focusing on beneficial probiotic lactic acid bacteria.
Tsukasa Shiraishi, Shinichi Yokota, Satoru Fukiya, Atsushi Yokota
Bioscience of microbiota, food and health, 35, 4, 147, 161, 2016, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Bacterial cell surface molecules are at the forefront of host-bacterium interactions. Teichoic acids are observed only in Gram-positive bacteria, and they are one of the main cell surface components. Teichoic acids play important physiological roles and contribute to the bacterial interaction with their host. In particular, lipoteichoic acid (LTA) anchored to the cell membrane has attracted attention as a host immunomodulator. Chemical and biological characteristics of LTA from various bacteria have been described. However, most of the information concerns pathogenic bacteria, and information on beneficial bacteria, including probiotic lactic acid bacteria, is insufficient. LTA is structurally diverse. Strain-level structural diversity of LTA is suggested to underpin its immunomodulatory activities. Thus, the structural information on LTA in probiotics, in particular strain-associated diversity, is important for understanding its beneficial roles associated with the modulation of immune response. Continued accumulation of structural information is necessary to elucidate the detailed physiological roles and significance of LTA. In this review article, we summarize the current state of knowledge on LTA structure, in particular the structure of LTA from lactic acid bacteria. We also describe the significance of structural diversity and biological roles of LTA.

Effect of pyruvate kinase gene deletion on the physiology of Corynebacterium glutamicum ATCC13032 under biotin-sufficient non-glutamate-producing conditions: Enhanced biomass production
Kazunori Sawada, Masaru Wada, Takuya Hagiwara, Susumu Zen-in, Keita Imai, Atsushi Yokota
Metabolic Engineering Communications, 2, 67, 75, Elsevier, 01 Dec. 2015, [Peer-reviewed]
English, Scientific journal

Structural insights into the substrate stereospecificity of D-threo-3-hydroxyaspartate dehydratase from Delftia sp HT23: a useful enzyme for the synthesis of optically pure L-threo- and D-erythro-3-hydroxyaspartate
Yu Matsumoto, Yoshiaki Yasutake, Yuki Takeda, Tomohiro Tamura, Atsushi Yokota, Masaru Wada
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99, 17, 7137, 7150, Sep. 2015, [Peer-reviewed]
English, Scientific journal

Activity of ERK regulates mucin 3 expression and is involved in undifferentiated Caco-2 cell death induced by 3-oxo-C-12-homoserine lactone
Hidehisa Shimizu, Nanako Baba, Takuma Nose, Ryoko Taguchi, Shinya Tanaka, Ga-Hyun Joe, Hideaki Maseda, Nobuhiko Nomura, Masahito Hagio, Ja-Young Lee, Satoru Fukiya, Atsushi Yokota, Satoshi Ishizuka, Hitoshi Miyazaki
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79, 6, 937, 942, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Isolation and transposition properties of ISBlo11, an active insertion sequence belonging to the IS3 family, from Bifidobacterium longum 105-A
Mikiyasu Sakanaka, Satoru Fukiya, Ryoko Kobayashi, Arisa Abe, Yosuke Hirayama, Yasunobu Kano, Atsushi Yokota
FEMS MICROBIOLOGY LETTERS, 362, 7, Apr. 2015, [Peer-reviewed]
English, Scientific journal

Diet supplementation with cholic acid promotes intestinal epithelial proliferation in rats exposed to gamma-radiation
Masahito Hagio, Hidehisa Shimizu, Ga-Hyun Joe, Manami Takatsuki, Maiko Shiwaku, Hong Xu, Ja-Young Lee, Nobuyuki Fujii, Satoru Fukiya, Hiroshi Hara, Atsushi Yokota, Satoshi Ishizuka
TOXICOLOGY LETTERS, 232, 1, 246, 252, Jan. 2015, [Peer-reviewed]
English, Scientific journal

Complete Genome Sequence of Bifidobacterium longum 105-A, a Strain with High Transformation Efficiency.
Yu Kanesaki, Hisayoshi Masutani, Mikiyasu Sakanaka, Yuh Shiwa, Takatomo Fujisawa, Yasukazu Nakamura, Atsushi Yokota, Satoru Fukiya, Tohru Suzuki, Hirofumi Yoshikawa
Genome announcements, 2, 6, 18 Dec. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bifidobacterium longum 105-A shows high transformation efficiency and allows for the generation of gene knockout mutants through homologous recombination. Here, we report the complete genome sequence of strain 105-A. Genes encoding at least four putative restriction-modification systems were found in this genome, which might contribute to its transformation efficiency.

Deoxycholic Acid Is Involved in the Proliferation and Migration of Vascular Smooth Muscle Cells
Hidehisa Shimizu, Masahito Hagio, Hitoshi Iwaya, Ikuya Tsuneki, Ja-Young Lee, Satoru Fukiya, Atsushi Yokota, Hitoshi Miyazaki, Hiroshi Hara, Satoshi Ishizuka
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 60, 6, 450, 454, Dec. 2014, [Peer-reviewed]
English, Scientific journal

Functional analysis of bifidobacterial promoters in Bifidobacterium longum and Escherichia coli using the alpha-galactosidase gene as a reporter
Mikiyasu Sakanaka, Saki Tamai, Yosuke Hirayama, Ai Onodera, Hiroka Koguchi, Yasunobu Kano, Atsushi Yokota, Satoru Fukiya
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 118, 5, 489, 495, Nov. 2014, [Peer-reviewed]
English, Scientific journal

The Combination of Functional Metagenomics and an Oil-Fed Enrichment Strategy Revealed the Phylogenetic Diversity of Lipolytic Bacteria Overlooked by the Cultivation-Based Method
Takashi Narihiro, Aya Suzuki, Kazuaki Yoshimune, Tomoyuki Hori, Tamotsu Hoshino, Isao Yumoto, Atsushi Yokota, Nobutada Kimura, Yoichi Kamagata
MICROBES AND ENVIRONMENTS, 29, 2, 154, 161, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a metabolic toggle switch
Yuki Soma, Keigo Tsuruno, Masaru Wada, Atsushi Yokota, Taizo Hanai
METABOLIC ENGINEERING, 23, 175, 184, May 2014, [Peer-reviewed]
English, Scientific journal

Contribution of the 7 beta-hydroxysteroid dehydrogenase from Ruminococcus gnavus N53 to ursodeoxycholic acid formation in the human colon
Ja-Young Lee, Hisashi Arai, Yusuke Nakamura, Satoru Fukiya, Masaru Wada, Atsushi Yokota
JOURNAL OF LIPID RESEARCH, 54, 11, 3062, 3069, Nov. 2013, [Peer-reviewed]
English, Scientific journal

Crystallization and preliminary X-ray diffraction studies of D-threo-3-hydroxyaspartate dehydratase isolated from Delftia sp HT23
Yu Matsumoto, Yoshiaki Yasutake, Yuki Takeda, Tomohiro Tamura, Atsushi Yokota, Masaru Wada
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 69, Pt 10, 1131, 1134, Oct. 2013, [Peer-reviewed]
English, Scientific journal

Lacto-N-biosidase Encoded by a Novel Gene of Bifidobacterium longum Subspecies longum Shows Unique Substrate Specificity and Requires a Designated Chaperone for Its Active Expression
Haruko Sakurama, Masashi Kiyohara, Jun Wada, Yuji Honda, Masanori Yamaguchi, Satoru Fukiya, Atsushi Yokota, Hisashi Ashida, Hidehiko Kumagai, Motomitsu Kitaoka, Kenji Yamamoto, Takane Katayama
JOURNAL OF BIOLOGICAL CHEMISTRY, 288, 35, 25194, 25206, Aug. 2013, [Peer-reviewed]
English, Scientific journal

Characterization of a Lactobacillus gasseri JCM 1131(T) Lipoteichoic Acid with a Novel Glycolipid Anchor Structure
Tsukasa Shiraishi, Shin-ichi Yokota, Naoki Morita, Satoru Fukiya, Satoru Tomita, Naoto Tanaka, Sanae Okada, Atsushi Yokota
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79, 10, 3315, 3318, May 2013, [Peer-reviewed]
English, Scientific journal

Development of a Double-Crossover Markerless Gene Deletion System in Bifidobacterium longum: Functional Analysis of the alpha-Galactosidase Gene for Raffinose Assimilation
Yosuke Hirayama, Mikiyasu Sakanaka, Hidenori Fukuma, Hiroki Murayama, Yasunobu Kano, Satoru Fukiya, Atsushi Yokota
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78, 14, 4984, 4994, Jul. 2012, [Peer-reviewed]
English, Scientific journal

Mechanism of increased respiration in an H+-ATPase-defective mutant of Corynebacterium glutamicum
Kazunori Sawada, Yui Kato, Keita Imai, Liyuan Li, Masaru Wada, Kazunobu Matsushita, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113, 4, 467, 473, Apr. 2012, [Peer-reviewed]
English, Scientific journal

Alterations of glucose metabolism in Escherichia coli mutants defective in respiratory-chain enzymes
Chie Kihira, Yukari Hayashi, Naoki Azuma, Sakiko Noda, Soya Maeda, Satoru Fukiya, Masaru Wada, Kazunobu Matsushita, Atsushi Yokota
JOURNAL OF BIOTECHNOLOGY, 158, 4, 215, 223, Apr. 2012
English, Scientific journal

Is bile acid a determinant of the gut microbiota on a high-fat diet?
Atsushi Yokota, Satoru Fukiya, Tadasuke Ooka, Yoshitoshi Ogura, Tetsuya Hayashi, Satoshi Ishizuka
Gut Microbes, 3, 5, 455, 459, Landes Bioscience, 2012, [Peer-reviewed]
English, Scientific journal

Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments.
Satoru Fukiya, Yosuke Hirayama, Mikiyasu Sakanaka, Yasunobu Kano, Atsushi Yokota
Bioscience of microbiota, food and health, 31, 2, 15, 25, 2012, [Peer-reviewed], [Domestic magazines]
English, Bifidobacteria are well known as beneficial intestinal bacteria that exert health-promoting effects in humans. In addition to physiological and immunological investigations, molecular genetic technologies have been developed and have recently started to be applied to clarify the molecular bases of host-Bifidobacterium interactions. These technologies include transformation technologies and Escherichia coli-Bifidobacterium shuttle vectors that enable heterologous gene expression. In this context, a plasmid artificial modification method that protects the introduced plasmid from the restriction system in host bifidobacteria has recently been developed to increase transformation efficiency. On the other hand, targeted gene inactivation systems, which are vital for functional genomics, seemed far from being practically applicable in bifidobacteria. However, remarkable progress in this technology has recently been achieved, enabling functional genomics in bifidobacteria. Integrated use of these molecular genetic technologies with omics-based analyses will surely boost characterization of the molecular basis underlying beneficial effects of bifidobacteria. Applications of recombinant bifidobacteria to medical treatments have also progressed.

Bile Acid Is a Host Factor That Regulates the Composition of the Cecal Microbiota in Rats
K. B. M. Saiful Islam, Satoru Fukiya, Masahito Hagio, Nobuyuki Fujii, Satoshi Ishizuka, Tadasuke Ooka, Yoshitoshi Ogura, Tetsuya Hayashi, Atsushi Yokota
GASTROENTEROLOGY, 141, 5, 1773, 1781, Nov. 2011
English, Scientific journal

Purification, characterization and amino acid sequence of a novel enzyme, D-threo-3-hydroxyaspartate dehydratase, from Delftia sp. HT23
Takayuki Maeda, Yuki Takeda, Tomoko Murakami, Atsushi Yokota, Masaru Wada
JOURNAL OF BIOCHEMISTRY, 148, 6, 705, 712, Dec. 2010
English, Scientific journal

Ingestion of Epilactose, a Non-digestible Disaccharide, Improves Postgastrectomy Osteopenia and Anemia in Rats through the Promotion of Intestinal Calcium and Iron Absorption
Takuya Suzuki, Megumi Nishimukai, Aki Shinoki, Hidenori Taguchi, Satoru Fukiya, Atsushi Yokota, Wataru Saburi, Takeshi Yamamoto, Hiroshi Hara, Hirokazu Matsui
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58, 19, 10787, 10792, Oct. 2010
English, Scientific journal

Characterization of an insertion sequence-like element, ISBlo15, identified in a size-increased cryptic plasmid pBK283 in Bifidobacterium longum BK28
Satoru Fukiya, Tomohiko Sugiyama, Yasunobu Kano, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 110, 2, 141, 146, Aug. 2010
English, Scientific journal

Metabolic changes in a pyruvate kinase gene deletion mutant of Corynebacterium glutamicum ATCC 13032
Kazunori Sawada, Susumu Zen-in, Masaru Wada, Atsushi Yokota
METABOLIC ENGINEERING, 12, 4, 401, 407, Jul. 2010
English, Scientific journal

One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase
Hiroto Kikuchi, Hiroaki Sakurai, Taizo Nagura, Tsutomu Aritsuka, Fusao Tomita, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 109, 3, 240, 243, Mar. 2010
English, Scientific journal

The nondigestible disaccharide epilactose increases paracellular Ca absorption via rho-associated kinase- and myosin light chain kinase-dependent mechanisms in rat small intestines.
Suzuki T, Nishimukai M, Takechi M, Taguchi H, Hamada S, Yokota A, Ito S, Hara H, Matsui H
Journal of agricultural and food chemistry, 58, 3, 1927, 1932, Feb. 2010, [Peer-reviewed]

Synbiotic promotion of epithelial proliferation by orally ingested encapsulated Bifidobacterium breve and raffinose in the small intestine of rats
Satoshi Ishizuka, Ami Iwama, Achmad Dinoto, Akarat Suksomcheep, Kohshi Maeta, Takanori Kasai, Hiroshi Hara, Atsushi Yokota
MOLECULAR NUTRITION & FOOD RESEARCH, 53, S62, S67, May 2009
English, Scientific journal

A novel membrane-anchored cytochrome c-550 of alkaliphilic Bacillus clarkii K24-1U: expression, molecular features and properties of redox potential
Ogami, Shinichi, Hijikata, Shoichi, Tsukahara, Tamotsu, Mie, Yasuhiro, Matsuno, Toshihide, Morita, Naoki, Hara, Isao, Yamazaki, Koji, Inoue, Norio, Yokota, Atsushi, Hoshino, Tamotsu, Yoshimune, Kazuaki, Yumoto, Isao
EXTREMOPHILES, 13, 3, 491, 504, May 2009
English, Scientific journal

Gene Cloning and Expression of Pyridoxal 5-Phosphate-Dependent L-threo-3-Hydroxyaspartate Dehydratase from Pseudomonas sp T62, and Characterization of the Recombinant Enzyme
Tomoko Murakami, Takayuki Maeda, Atsushi Yokota, Masaru Wada
JOURNAL OF BIOCHEMISTRY, 145, 5, 661, 668, May 2009
English, Scientific journal

Conversion of cholic acid and chenodeoxycholic acid into their 7-oxo derivatives by Bacteroides intestinalis AM-1 isolated from human feces
Satoru Fukiya, Miki Arata, Hiroko Kawashima, Daisuke Yoshida, Maki Kaneko, Kimiko Minamida, Jun Watanabe, Yoshio Ogura, Kiyohisa Uchida, Kikuji Itoh, Masaru Wada, Susumu Ito, Atsushi Yokota
FEMS MICROBIOLOGY LETTERS, 293, 2, 263, 270, Apr. 2009
English, Scientific journal

Industrial production of difructose anhydride III (DFA III) from crude inulin extracted from chicory roots using Arthrobacter sp H65-7 fructosyltransferase
Hiroto Kikuchi, Masanao Inoue, Hidetoshi Saito, Hiroaki Sakurai, Tsutomu Aritsuka, Fusao Tomita, Atsushi Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 107, 3, 262, 265, Mar. 2009
English, Scientific journal

Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
Takeshi Senoura, Hidenori Taguchi, Shigeaki Ito, Shigeki Hamada, Hirokazu Matsui, Satoru Fukiya, Atsushi Yokota, Jun Watanabe, Jun Wasaki, Susumu Ito
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73, 2, 400, 406, Feb. 2009
English, Scientific journal

Enhanced Valine Production in Corynebacterium glutamicum with Defective H+-ATPase and C-Terminal Truncated Acetohydroxyacid Synthase
Masaru Wada, Nowaki Hijikata, Ryo Aoki, Nobuchika Takesue, Atsushi Yokota
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 72, 11, 2959, 2965, Nov. 2008
English, Scientific journal

[FISH-flow cytometry, a new tool for the analysis of intestinal microbiota].
Fukiya S, Yokota A
Seikagaku. The Journal of Japanese Biochemical Society, 80, 5, 421, 425, 5, 25 May 2008, [Peer-reviewed]
Japanese

Change of proteins in instant Chinese noodle by the fermentation of Lactobacillus plantarum NRIC 0380 affects the noodle quality
Eriko Yahata, Yuki Sawatari, Hisashi Sugiyama, Akihiro Hanaoka, Atsushi Yokota, Hamo Saruyama
Food Science and Technology Research, 14, 3, 285, 292, May 2008, [Peer-reviewed]
English, Scientific journal

Change of proteins in instant Chinese noodle by the fermentation of Lactobacillus plantarum NRIC 0380 affects the noodle quality
Eriko Yahata, Yuki Sawatari, Hisashi Sugiyama, Akihiro Hanaoka, Atsushi Yokota, Haruo Saruyama
FOOD SCIENCE AND TECHNOLOGY RESEARCH, 14, 3, 285, 292, May 2008
English, Scientific journal

Distribution of L-azetidine-2-carboxylate N-acetyltransferase in yeast
Masaru Wada, Kae Okabe, Michihiko Kataoka, Sakayu Shimizu, Atsushi Yokota, Hiroshi Takagi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 72, 2, 582, 586, Feb. 2008
English, Scientific journal

Cytoplasmic proteome reference map for a glutamic acid-producing Corynebacterium glutamicum ATCC 14067
Liyuan Li, Masaru Wada, Atsushi Yokota
PROTEOMICS, 7, 23, 4317, 4322, Dec. 2007
English, Scientific journal

Alanine production in an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli expressing alanine dehydrogenase
Masaru Wada, Kotomi Narita, Atsushi Yokota
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 76, 4, 819, 825, Sep. 2007
English, Scientific journal

A comparative proteomic approach to understand the adaptations of an H+-ATPase-defective mutant of Corynebacterium glutamicum ATCC14067 to energy deficiencies
Liyuan Li, Masaru Wada, Atsushi Yokota
PROTEOMICS, 7, 18, 3348, 3357, Sep. 2007
English, Scientific journal

Erratum: Diversity and mechanisms of alkali tolerance in lactobacilli (Applied and Environmental Microbiology (2007) 73,12, (3909-3915))
Yuki Sawatari, Atsushi Yokota
Applied and Environmental Microbiology, 73, 16, 5385, Aug. 2007, [Peer-reviewed]
English, Scientific journal

Diversity and mechanisms of alkali tolerance in lactobacilli
Yuki Sawatari, Atsushi Yokota
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73, 12, 3909, 3915, Jun. 2007
English, Scientific journal

Yoghurt fermented by Lactobacillus delbrueckii subsp bulgaricus H+-ATPase-defective mutants exhibits enhanced viability of Bifidobacterium breve during storage.
Martin Patrick Ongol, Yaki Sawatani, Yoshiko Ebina, Teruo Sone, Michiko Tanaka, Fusao Tomita, Atsushi Yokota, Kozo Asano
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 116, 3, 358, 366, May 2007
English, Scientific journal

FISH-Flow Cytometry : A High-Throughput Molecular Ecological Analysis of Intestinal Microbiota
DINOTO Achmad, FUKIYA Satoru, YOKOTA Atsushi
Japanese Journal of Lactic Acid Bacteria, 17, 2, 110, 117, 01 Dec. 2006
English

Development of food grade media for the preparation of Lactobacillus plantarum starter culture
Yuki Sawatari, Tomomi Hirano, Atsushi Yokota
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 52, 6, 349, 356, Dec. 2006
English, Scientific journal

Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry
Achmad Dinoto, Tatiana M. Marques, Kanta Sakamoto, Satoru Fukiya, Jun Watanabe, Susumu Ito, Atsushi Yokota
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72, 12, 7739, 7747, Dec. 2006
English, Scientific journal

Alterations of cellular physiology in Escherichia coli in response to oxidative phosphorylation impaired by defective F-1-ATPase
Sakiko Noda, Yuji Takezawa, Tomohiko Mizutani, Tomoaki Asakura, Eiichiro Nishiumi, Kazunori Onoe, Masaru Wada, Fusao Tomita, Kazunobu Matsushita, Atsushi Yokota
JOURNAL OF BACTERIOLOGY, 188, 19, 6869, 6876, Oct. 2006
English, Scientific journal

Mechanism of growth inhibition by free bile acids in lactobacilli and bifidobacteria
P Kurdi, K Kawanishi, K Mizutani, A Yokota
JOURNAL OF BACTERIOLOGY, 188, 5, 1979, 1986, Mar. 2006
English, Scientific journal

Modulation of rat cecal microbiota by administration of raffinose and encapsulated Bifidobacterium breve
A Dinoto, A Suksomcheep, S Ishizuka, H Kimura, S Hanada, Y Kamagata, K Asano, F Tomita, A Yokota
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72, 1, 784, 792, Jan. 2006
English, Scientific journal

Development of fermented instant Chinese noodle using Lactobacillus plantarum
Y Sawatari, H Sugiyama, Y Suzuki, A Hanaoka, K Saito, H Yamauchi, S Okada, A Yokota
FOOD MICROBIOLOGY, 22, 6, 539, 546, Dec. 2005
English, Scientific journal

Development and assessment of a real-time PCR assay for rapid and sensitive detection of a novel thermotolerant bacterium, Lactobacillus thermotolerans, in chicken feces
ASM Selim, P Boonkumklao, T Sone, A Assavanig, M Wada, A Yokota
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71, 8, 4214, 4219, Aug. 2005
English, Scientific journal

Enhanced glutamic acid production by a H+-ATPase-defective mutant of Corynebacterium glutamicum
R Aoki, M Wada, N Takesue, K Tanaka, A Yokota
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 69, 8, 1466, 1472, Aug. 2005
English, Scientific journal

Effects of difructose anhydride III (DFA III) administration on bile acids and growth of DFA III-assimilating bacterium Ruminococcus productus on rat intestine.
Minamida K, Kaneko M, Ohashi M, Sujaya IN, Sone T, Wada M, Yokota A, Hara H, Asano K, Tomita F
Journal of bioscience and bioengineering, 99, 6, 548, 554, The Society for Biotechnology, Japan, Jun. 2005, [Peer-reviewed]
English, Scientific journal, The growth of DFA III-assimilating bacteria in the intestines of rats fed 3% DFA III for 2 weeks was examined. Sixty-four percent of the DFA III intake had been assimilated on day 3 of ingestion, and almost all of the DFA III was assimilated at the end of the experiment. The DFA III-assimilating bacterium, Ruminococcus productus, in DFA III-fed rats was in the stationary state of 10⁸–10⁹ cells/g dry feces within a week from 10⁶ cells/g dry feces on day 1 of DFA III ingestion. The number of R. productus cells was associated with the amount of DFA III excreted in the feces. The acetic acid produced from DFA III by R. productus lowered the cecal pH to 5.8. In control-fed rats and DFA III-fed rats, 94% of secondary bile acids and 94% of primary bile acids, respectively, were accounted for in the total bile acids analyzed. DFA III ingestion increased the ratio of primary bile acids and changed the composition of fecal bile acids. In conclusion, R. productus assimilated DFA III, produced short chain fatty acids, and the cecal pH was lowered. The acidification of rat intestine perhaps inhibited secondary bile acid formation and decreased the ratio of secondary bile acids. Therefore, it is expected that DFA III may prevent colorectal cancer and be a new prebiotic candidate.

Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
Minamida K, Shiga K, Sujaya IN, Sone T, Yokota A, Hara H, Asano K, Tomita F
Journal of bioscience and bioengineering, 99, 3, 230, 236, The Society for Biotechnology, Japan, Mar. 2005, [Peer-reviewed]
English, Scientific journal, The effects of difructose anhydride III (di-D-fructofuranose-1,2′:2,3′-dianhydride; DFA III) administration (3% DFA III for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (DGGE). According to DGGE profiles, the number of bacteria related to Bacteroides acidofaciens and uncultured bacteria within the Clostridium lituseburense group decreased, while that of bacteria related to Bacteroides vulgatus, Bacteroides uniformis and Ruminococcus productus increased in DFA III-fed rat cecum. In the cecal contents of DFA III-fed rats, a lowering of pH and an increase in short chain fatty acids (SCFAs), especially acetic acid, were observed. The DFA III-assimilating bacterium, Ruminococcus sp. M-1, was isolated from the cecal contents of DFA III-fed rats. The strain had 98% similarity with R. productus ATCC 27340^T (L76595), and mainly produced acetic acid. These results confirmed that the bacteria harmful to host health were not increased by DFA III administration. Moreover, DFA III stimulated the growth of Ruminococcus sp. M-1 producing acetic acid, which may alter the intestinal microbiota towards a healthier composition. It is expected that DFA III would be a new candidate as a prebiotic.

Development of a novel fermented food from wheat flour:Fermented instant Chinese noodle using plant-origin lactobacilli
Sawatari Yuki, Yokota Atsushi
Japanese Journal of Lactic Acid Bacteria, 16, 2, 75, 83, 日本乳酸菌学会誌, 2005, [Peer-reviewed]
Japanese, We have been engaged in the development of a novel fermented food from wheat flour &ldquo;fermented instant Chinese noodl&rdquo; using lactic acid bacterial fermentation. A few fermented foods from wheat flour using lactic acid bacterial fermentation are known. The only well known products are sourdough breads. Since the pH value of the noodle environment is alkaline with <I>kansui</I> (around 8.5), alkaline tolerance is required for the lactic acid bacteria to ferment noodle dough. The screening of the lactic acid bacterial strains suitable for the fermentation was conducted using 46 strains from 12 species (including subspecies)of lactobacilli isolated mostly from plant-materials. Positive results were obtained only with <I>Lactobacillus</I> <I>(Lb.)</I> pentosus and Lb. plantarum strains. The fermented instant Chinese noodle prepared by the bestselected strain, <I>Lb. plantarum</I> NRIC 0380, showed improved noodle texture with a light sour taste. The relationship between the food chemical characteristics of the noodle dough and physiological characteristics of lactobacilli are also discussed.

The effects of di-D-fructofuranose-1,2 ': 2,3 '-dianhydride (DFA III) administration on human intestinal microbiota
K Minamida, IN Sujaya, A Tamura, N Shigematsu, T Sone, A Yokota, K Asano, Y Benno, F Tomita
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 98, 4, 244, 250, Oct. 2004
English, Scientific journal

Isolation of a cadmium-releasing bacterium and characterization of its novel protease
S Ren, F Tomita, A Yokota, K Asano
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68, 8, 1627, 1633, Aug. 2004, [Peer-reviewed]
English, Scientific journal

Effects of indigenous starter cultures on the microbial and physicochemical characteristics of Urutan, a Balinese fermented sausage
NS Antara, IN Sujaya, A Yokota, K Asano, F Tomita
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 98, 2, 92, 98, Aug. 2004, [Peer-reviewed]
English, Scientific journal

Identification and characterization of yeasts in brem, a traditional Balinese rice wine
IN Sujaya, NS Antara, T Sone, Y Tamura, WR Aryanta, A Yokota, K Asano, F Tomita
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 20, 2, 143, 150, Mar. 2004
English, Scientific journal

Physical, chemical and physiological properties of difructose anhydride III produced from inulin by enzymatic reaction
KIKUCHI H.
J. Appl. Glycosci., 51, 4, 291, 296, 2004

Development of internal transcribed spacer regions amplification restriction fragment length polymorphism method and its application in monitoring the population of Zygosaccharomyces rouxii M2 in miso fermentation
IN Sujaya, Y Tamura, T Tanaka, T Yamaki, T Ikeda, N Kikushima, H Yata, A Yokota, K Asano, F Tomita
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 96, 5, 438, 447, Nov. 2003
English, Scientific journal

Cholic acid accumulation and its diminution by short-chain fatty acids in bifidobacteria
P Kurdi, H Tanaka, HW van Veen, K Asano, F Tomita, A Yokota
MICROBIOLOGY-SGM, 149, 8, 2031, 2037, Aug. 2003
English, Scientific journal

Molecular cloning of the gene encoding the di-D-fructofuranose 1,2 ': 2,3 ' dianhydride hydrolysis enzyme (DFA IIIase) from Arthrobacter sp H65-7
K Saito, Y Sumita, Y Nagasaka, F Tomita, A Yokota
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 95, 5, 538, 540, May 2003
English, Scientific journal

Bacterial adjunct to improve the quality of alkaline noodles
K Saito, Z Nishio, K Takata, T Kuwabara, A Yokota, H Yamauchi, Y Oda
FOOD SCIENCE AND TECHNOLOGY RESEARCH, 9, 1, 40, 44, Feb. 2003
English, Scientific journal

Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces
P Niamsup, IN Sujaya, M Tanaka, T Sone, S Hanada, Y Kamagata, S Lumyong, A Assavanig, K Asano, F Tomita, A Yokota
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 53, 1, 263, 268, Jan. 2003
English, Scientific journal

Molecular cloning of the gene for 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2
K Saito, Y Oda, F Tomita, A Yokota
FEMS MICROBIOLOGY LETTERS, 218, 2, 265, 270, Jan. 2003
English, Scientific journal

Production of lepidimoide by an endophytic fungus from polysaccharide extracted from Abelmoschus sp.: Identification of the product and the organism producing it
M Tanaka, M Yoshimura, M Suto, A Yokota, K Asano, E Sukara, F Tomita
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93, 6, 531, 536, Jun. 2002
English, Scientific journal

Specific enumeration of lactic acid bacteria in ragi tape by colony hybridization with specific oligonucleotide probes
IN Sujaya, S Amachi, K Saito, A Yokota, K Asano, F Tomita
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 18, 3, 263, 270, Apr. 2002
English, Scientific journal

Identification and succession of lactic acid bacteria during fermentation of 'urutan', a Balinese indigenous fermented sausage
NS Antara, IN Sujaya, A Yokota, K Asano, WR Aryanta, F Tomita
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 18, 3, 255, 262, Apr. 2002
English, Scientific journal

Endophytes as producers of xylanase
M Suto, M Takebayashi, K Saito, M Tanaka, A Yokota, F Tomita
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93, 1, 88, 90, Jan. 2002, [Peer-reviewed]
English, Scientific journal

H+-ATPase defect in Corynebacterium glutamicum abolishes glutamic acid production with enhancement of glucose consumption rate
H Sekine, T Shimada, C Hayashi, A Ishiguro, F Tomita, A Yokota
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 57, 4, 534, 540, Nov. 2001
English, Scientific journal

Identification and characterization of lactic acid bacteria in ragi tape
IN Sujaya, S Amachi, A Yokota, K Asano, F Tomita
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 17, 4, 349, 357, Jun. 2001
English, Scientific journal

Microbial demetallization of crude oil: Nickel protoporphyrin disodium as a model organo-metallic substrate
GR Dedeles, A Abe, K Saito, K Asano, K Saito, A Yokota, F Tomita
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 90, 5, 515, 521, Nov. 2000
English, Scientific journal

Cholic acid is accumulated spontaneously, driven by membrane Delta pH, in many lactobacilli
P Kurdi, HW van Veen, H Tanaka, Mierau, I, WN Konings, GW Tannock, F Tomita, A Yokota
JOURNAL OF BACTERIOLOGY, 182, 22, 6525, 6528, Nov. 2000
English, Scientific journal

Cholate resistance in Lactococcus lactis is mediated by an ATP-dependent multispecific organic anion transporter
A Yokota, M Veenstra, P Kurdi, HW van Veen, WN Konings
JOURNAL OF BACTERIOLOGY, 182, 18, 5196, 5201, Sep. 2000
English, Scientific journal

Molecular cloning and high-level expression in Escherichia coli of fungal alpha-galactosidase from Absidia corymbifera IFO 8084
SH Baik, K Saito, A Yokota, K Asano, F Tomita
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 90, 2, 168, 173, Aug. 2000
English, Scientific journal

Purification and characterization of 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2
A Saito, K Kondo, Kojima, I, A Yokota, F Tomita
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66, 1, 252, 256, Jan. 2000
English, Scientific journal

An antifungal compound from roots of Welsh onion
N Phay, T Higashiyama, M Tsuji, H Matsuura, Y Fukushi, A Yokota, F Tomita
PHYTOCHEMISTRY, 52, 2, 271, 274, Sep. 1999
English, Scientific journal

A novel screen for the detection of chitin acting antifungal compounds
T Sakurai, N Cheeptham, T Mikawa, A Yokota, F Tomita
JOURNAL OF ANTIBIOTICS, 52, 5, 508, 511, May 1999
English

Effects of DFA IV in rats: Calcium absorption and metabolism of DFA IV by intestinal microorganisms
K Saito, T Hira, T Suzuki, H Hara, A Yokota, F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 63, 4, 655, 661, Apr. 1999
English, Scientific journal

Studies on an antifungi antibiotic from Ellisiodothis inguinans L-1588-A8
NAOWARAT C.
Thai J. Biotechnol., 1, 1, 37, 45, 1999

Purification and properties of the raw-starch-digesting glucoamylases from Corticium rolfsii
Y Nagasaka, K Kurosawa, A Yokota, F Tomita
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 50, 3, 323, 330, Sep. 1998
English, Scientific journal

Characterization of a mutant of Lactococcus lactis with reduced membrane-bound ATPase activity under acidic conditions
S Amachi, K Ishikawa, S Toyoda, Y Kagawa, A Yokota, F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62, 8, 1574, 1580, Aug. 1998
English, Scientific journal

Molecular cloning of levan fructotransferase gene from Arthrobacter nicotinovorans GS-9 and its expression in Escherichia coli
K Saito, A Yokota, F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 61, 12, 2076, 2079, Dec. 1997
English, Scientific journal

Purification of levan fructotransferase from Arthrobacter nicotinovorans GS-9 and production of DFA IV from levan by the enzyme
K Saito, H Goto, A Yokota, F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 61, 10, 1705, 1709, Oct. 1997
English, Scientific journal

Metabolism of DFA III by Arthrobacter sp. H65-7: Purification and properties of a DFA III hydrolysis enzyme (DFA IIIase)
H Sakurai, A Yokota, Y Sumita, Y Mori, H Matsui, F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 61, 6, 989, 993, Jun. 1997
English, Scientific journal

Breeding of useful microorganisms by mutation in energy metabolism
Atsushi Yokota
Nippon Nogeikagaku Kaishi, 71, 1, 9, 14, Japan Society for Bioscience Biotechnology and Agrochemistry, 1997, [Peer-reviewed]
Japanese, Scientific journal

Enhancement of glucose metabolism in a pyruvic acid-hyperproducing Escherichia coli mutant defective in F-1-ATPase activity
A Yokota, M Henmi, N Takaoka, C Hayashi, Y Takezawa, Y Fukumori, F Tomita
JOURNAL OF FERMENTATION AND BIOENGINEERING, 83, 2, 132, 138, 1997
English, Scientific journal

Molecular cloning of an inulin fructotransferase (depolymerizing) gene from Arthrobacter sp H65-7 and its expression in Escherichia coli
H Sakurai, A Yokota, F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 61, 1, 87, 92, Jan. 1997
English, Scientific journal

NP-101A, antifungal antibiotic from Streptomyces aurantiogriseus NPO-101
N Phay, H Yada, T Higashiyama, A Yokota, A Ichihara, F Tomita
JOURNAL OF ANTIBIOTICS, 49, 7, 703, 705, Jul. 1996
English, Scientific journal

L-tryptophan production by a pyruvic acid-producing Escherichia coli strain carrying the Enterobacter aerogenes tryptophanase gene
K Kawasaki, A Yokota, F Tomita
JOURNAL OF FERMENTATION AND BIOENGINEERING, 82, 6, 604, 606, 1996
English, Scientific journal

Cloning of Corticium rolfsii glucoamylase cDNA and its expression in Saccharomyces cerevisiae
Y Nagasaka, N Muraki, A Kimura, M Suto, A Yokota, F Tomita
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 44, 3-4, 451, 458, Dec. 1995
English, Scientific journal

PRODUCTION OF INULOTRIOSE FROM INULIN BY INULIN-DEGRADING ENZYME FROM STREPTOMYCES-ROCHEI-E87
A YOKOTA, O YAMAUCHI, F TOMITA
LETTERS IN APPLIED MICROBIOLOGY, 21, 5, 330, 333, Nov. 1995
English, Scientific journal

ACID SENSITIVITY OF A MUTANT OF LACTOCOCCUS-LACTIS SUBSP LACTIS C2 WITH REDUCED MEMBRANE-BOUND ATPASE ACTIVITY
A YOKOTA, S AMACHI, S ISHII, F TOMITA
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 59, 10, 2004, 2007, Oct. 1995
English, Scientific journal

ENZYMATIC-SYNTHESIS OF L-TRYPTOPHAN BY ENTEROBACTER-AEROGENES TRYPTOPHANASE HIGHLY EXPRESSED IN ESCHERICHIA-COLI, AND SOME PROPERTIES OF THE PURIFIED ENZYME
K KAWASAKI, A YOKOTA, F TOMITA
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 59, 10, 1938, 1943, Oct. 1995
English, Scientific journal

Microbial Degradation of Quinoline in Heavy Oil
Toshiyuki Obara, Atsushi Yokota, Fusao Tomita
Nihon Enerugi Gakkaishi/Journal of the Japan Institute of Energy, 74, 4, 228, 232, 1995
English, Scientific journal

PYRUVIC-ACID PRODUCTION BY AN F-1-ATPASE-DEFECTIVE MUTANT OF ESCHERICHIA-COLI W1485LIP2
A YOKOTA, Y TERASAWA, N TAKAOKA, H SHIMIZU, F TOMITA
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 58, 12, 2164, 2167, Dec. 1994
English, Scientific journal

PYRUVIC-ACID PRODUCTION BY A LIPOIC ACID AUXOTROPH OF ESCHERICHIA-COLI W1485
A YOKOTA, H SHIMIZU, Y TERASAWA, N TAKAOKA, F TOMITA
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 41, 6, 638, 643, Aug. 1994
English, Scientific journal

CLONING AND CHARACTERIZATION OF A TRYPTOPHANASE GENE FROM ENTEROBACTER-AEROGENES SM-18
K KAWASAKI, A YOKOTA, S OITA, C KOBAYASHI, S YOSHIKAWA, SI KAWAMOTO, S TAKAO, F TOMITA
JOURNAL OF GENERAL MICROBIOLOGY, 139, 12, 3275, 3281, Dec. 1993
English, Scientific journal

PRODUCTION OF LEVANBIOSE BY A LEVAN-DEGRADING ENZYME FROM STREPTOMYCES-EXFOLIATUS F3-2
A YOKOTA, K KONDO, M NAKAGAWA, KOJIMA, I, F TOMITA
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 57, 5, 745, 749, May 1993
English, Scientific journal

MECHANISM OF ACTION OF INHIBITORS FOR BACTERIAL OUTER-MEMBRANE
F TOMITA, A YOKOTA
NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 65, 6, 998, 1002, Jun. 1991, [Peer-reviewed]
Japanese, Scientific journal

PRODUCTION OF INULIN FRUCTOTRANSFERASE (DEPOLYMERIZING) BY ARTHROBACTER SP H65-7 AND PREPARATION OF DFA-III FROM INULIN BY THE ENZYME
A YOKOTA, S HIRAYAMA, K ENOMOTO, Y MIURA, S TAKAO, F TOMITA
JOURNAL OF FERMENTATION AND BIOENGINEERING, 72, 4, 258, 261, 1991
English, Scientific journal

PURIFICATION AND PROPERTIES OF AN INULIN FRUCTOTRANSFERASE (DEPOLYMERIZING) FROM ARTHROBACTER SP H65-7
A YOKOTA, K ENOMOTO, F TOMITA
JOURNAL OF FERMENTATION AND BIOENGINEERING, 72, 4, 262, 265, 1991
English, Scientific journal

ETHANOL-PRODUCTION FROM RAW STARCH BY SIMULTANEOUS FERMENTATION USING SCHIZOSACCHAROMYCES-POMBE AND A RAW STARCH SACCHARIFYING ENZYME FROM CORTICIUM-ROLFSII
J HARIANTONO, A YOKOTA, S TAKAO, F TOMITA
JOURNAL OF FERMENTATION AND BIOENGINEERING, 71, 5, 367, 369, 1991
English

DIHYDRODIPICOLINATE SYNTHASE DEFICIENCY OF BREVIBACTERIUM-FLAVUM STRAIN-BB69, A THREONINE-PRODUCING MUTANT WITH A FEEDBACK-RESISTANT HOMOSERINE DEHYDROGENASE
A YOKOTA, SHIIO, I
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 54, 2, 547, 548, Feb. 1990
English

ENZYMATIC PRODUCTION OF TRYPTOPHAN USING A LIPOIC ACID AND THIAMINE DOUBLE AUXOTROPH OF ENTEROBACTER-AEROGENES HAVING BOTH PYRUVIC-ACID PRODUCTIVITY AND HIGH TRYPTOPHANASE ACTIVITY
S OITA, A YOKOTA, S TAKAO
JOURNAL OF FERMENTATION AND BIOENGINEERING, 69, 4, 256, 258, 1990
English

ISOLATION AND PROPERTIES OF DIHYDRODIPICOLINATE SYNTHASE-DEFECTIVE THREONINE-PRODUCING MUTANTS FROM A BREVIBACTERIUM-FLAVUM STRAIN WITH FEEDBACK-SENSITIVE ASPARTOKINASE
SHIIO, I, A YOKOTA, K KAWAMURA
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 53, 8, 2169, 2175, Aug. 1989
English, Scientific journal

TRYPTOPHAN PRODUCTION BY A LIPOIC ACID AUXOTROPH OF ENTEROBACTER-AEROGENES HAVING BOTH PYRUVIC-ACID PRODUCTIVITY AND HIGH TRYPTOPHANASE ACTIVITY
A YOKOTA, S OITA, S TAKAO
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 53, 8, 2037, 2044, Aug. 1989
English, Scientific journal

PYRUVIC-ACID PRODUCTION BY LIPOIC ACID AUXOTROPHS OF ENTEROBACTER-AEROGENES
A YOKOTA, S TAKAO
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 53, 3, 705, 711, Mar. 1989
English, Scientific journal

THREONINE PRODUCTION BY DIHYDRODIPICOLINATE SYNTHASE-DEFECTIVE MUTANTS OF BREVIBACTERIUM-FLAVUM
SHIIO, I, A YOKOTA, Y TORIDE, SI SUGIMOTO
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 53, 1, 41, 48, Jan. 1989
English, Scientific journal

EFFECTS OF REDUCED CITRATE SYNTHASE ACTIVITY AND FEEDBACK-RESISTANT PHOSPHOENOLPYRUVATE CARBOXYLASE ON LYSINE PRODUCTIVITIES OF BREVIBACTERIUM-FLAVUM MUTANTS
A YOKOTA, SHIIO, I
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 52, 2, 455, 463, Feb. 1988
English, Scientific journal

EFFECT OF PYRUVATE-KINASE DEFICIENCY ON L-LYSINE PRODUCTIVITIES OF MUTANTS WITH FEEDBACK-RESISTANT ASPARTOKINASES
SHIIO, I, A YOKOTA, S SUGIMOTO
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 51, 9, 2485, 2493, Sep. 1987
English, Scientific journal

CONVERSION OF PYRUVIC-ACID FERMENTATION TO TRYPTOPHAN PRODUCTION BY THE COMBINATION OF PYRUVIC ACID-PRODUCING MICROORGANISMS AND ENTEROBACTER-AEROGENES HAVING HIGH TRYPTOPHANASE ACTIVITY
A YOKOTA, S TAKAO
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 48, 11, 2663, 2668, 1984
English, Scientific journal

ENZYMATIC PRODUCTION OF TRYPTOPHAN COUPLED TO PYRUVIC-ACID FERMENTATION
S TAKAO, A YOKOTA, M TANIDA
JOURNAL OF FERMENTATION TECHNOLOGY, 62, 4, 329, 334, 1984
English, Scientific journal

L-MALIC ACID FERMENTATION BY A MIXED CULTURE OF RHIZOPUS-ARRHIZUS AND PAECILOMYCES-VARIOTI
S TAKAO, A YOKOTA, M TANIDA
JOURNAL OF FERMENTATION TECHNOLOGY, 61, 6, 643, 645, 1983
English

高脂肪食摂取ラット盲腸で増加した遊離脂肪酸種が腸内細菌に与える影響の評価
川上志野, 川上憲太郎, GOWDA Siddabasave Gowda B., CHEN Zhen, 吹谷智, LIANG Chongsheng, HAI Han, 石塚敏, 小椋義俊, 後藤恭宏, 前田智也, 林哲也, 千葉仁志, 惠淑萍, 横田篤, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2023, 2023

Elucidation of the effects of the fatty acid profile changes on the gut microbiota composition in the cecum of rats fed a high-fat diet: validation of a fatty-acid hypothesis
川上憲太郎, GOWDA Siddabasave Gowda B., CHEN Zhen, 吹谷智, LIANG Chongsheng, HAI Han, 石塚敏, 小椋義俊, 後藤恭宏, 前田智也, 林哲也, 千葉仁志, 惠淑萍, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2022, 2022

Elucidation of the metabolic mechanism of 1-Kestose in Bifidobacterium longum subsp. longum
難波有希, 倉持碧海, 山口颯人, 吉田佳鼓, 後藤恭宏, 門田吉弘, 栃尾巧, 小椋義俊, 林哲也, 前田智也, 横田篤, 吹谷智, 日本乳酸菌学会誌, 33, 2, 2022

高脂肪食摂取ラットにおける盲腸内脂肪酸プロファイルの変化が腸内細菌叢構成に与える影響
川上憲太郎, GOWDA B. Gowda Siddabasave, CHEN Zhen, 吹谷智, LIANG Chongsheng, HAI Han, 石塚敏, 小椋義俊, 後藤恭宏, 前田智也, 林哲也, 千葉仁志, 惠淑萍, 横田篤, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2021, 2021

Elucidation of the metabolic mechanism of 1-Kestose in Bifidobacterium longum subsp. longum
難波有希, 山口颯人, 吉田佳鼓, 後藤恭宏, 門田吉弘, 栃尾巧, 小椋義俊, 林哲也, 横田篤, 吹谷智, 日本生物工学会大会講演要旨集, 73rd, 2021

Comparison of contributions of pyruvate carboxylase and phosphoenolpyruvate carboxylase reactions on lysine production by Corynebacterium glutamicum
新森彰信, GUO Zhen, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2021, 2021

Elucidation of the mechanism of enhanced lysine production by mutation in citrate synthase gene leading to its reduced activity in Corynebacterium glutamicum
大塚萌音, GUO Zhen, 佐藤桃梨, 愛甲徹, 柳瀬真紀, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2021, 2021

Identification of enzyme genes involved in reverse transsulfuration pathway in Bifidobacterium longum 105-A using gene-knockout mutants
宮森那知, 松本菜々恵, 吹谷智, 横田篤, 和田大, 日本農芸化学会大会講演要旨集(Web), 2021, 2021

Evaluation of the bifidobacterial genes for contribution to colonization and survival in the conventional mouse intestine as identified by Insertion-Sequencing
井上太貴, 横田篤, 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2021, 2021

Identification of bifidobacterial genes that contribute to survival and colonization in the gastrointestinal tract of conventional mice using insertion-sequencing.
山口颯人, 倉持碧海, 後藤恭宏, 小椋義俊, 園山慶, 林哲也, 横田篤, 吹谷智, 日本乳酸菌学会誌, 32, 2, 2021

Mucin O-glycan assimilation pathway in Bifidobacterium
CHOI Moonhak, 後藤愛那, 阪中幹祥, 加藤紀彦, 西本完, 杉山友太, 栗原新, 吹谷智, 横田篤, 北岡本光, 片山高嶺, 日本農芸化学会大会講演要旨集(Web), 2021, 2021

Identification and functional verification of enzyme genes constituting reverse transsulfuration pathway in Bifidobacterium longum 105-A
宮森那知, 松本菜々恵, 吹谷智, 横田篤, 和田大, 日本乳酸菌学会誌, 32, 2, 2021

Bile acid as a responsible factor for high-fat diet-induced gut microbiota alterations: Proof of “bile acid hypothesis“
横田篤, 渡辺真通, 藤田祐己, 石塚敏, 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2020, 2020

Association between 12α-hydroxylated bile acids and hepatic steatosis
堀将太, 阿部隆行, LEE DongGeun, 吹谷智, 横田篤, 麻生菜央, 城内文吾, 佐藤匡央, 石塚敏, 日本農芸化学会大会講演要旨集(Web), 2020, 2020

エクオール産生におよぼす胆汁酸の影響
吉岡寛子, 渡辺真通, 難波文男, 鈴木利雄, 吹谷智, 横田篤, 戸田登志也, 日本農芸化学会大会講演要旨集(Web), 2019, ROMBUNNO.4C1a11 (WEB ONLY), 05 Mar. 2019
Japanese

The membrane phospholipid cardiolipin plays a pivotal role in bile acid adaptation by Lactobacillus gasseri JCM1131 ^T
Shinji Kato, Haruhi Tobe, Hiroki Matsubara, Mariko Sawada, Yasuko Sasaki, Satoru Fukiya, Naoki Morita, Atsushi Yokota, Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1864, 403, 412, 01 Mar. 2019

ビフィズス菌のトランスポゾン変異株を用いたα-グルコシダーゼ遺伝子の機能解明
阪中幹祥, 阪中幹祥, 阪中幹祥, 中川路伸吾, 中島森, 阿部光紗, 佐分利亘, 森春英, 横田篤, 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2019, 2019

高脂肪食中に含まれる脂肪酸がラット盲腸内細菌叢構成に与える影響の解明:脂肪酸仮説の検証
阿部隆行, 渡辺真通, 藤田祐己, 小椋義俊, 吹谷智, 石塚敏, 林哲也, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2019, 2019

高脂肪食またはコール酸摂取マウス腸内における胆汁酸組成および細菌叢構成の動態解析による胆汁酸仮説の検証
坂本佳奈子, 相磯知里, 松浦史佳, 吹谷智, 園山慶, 小椋義俊, 林哲也, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2019, 2019

胆汁酸変換菌の共培養によるウルソコール酸からデオキシコール酸への変換機構の検証
北川更, TAWTHEP Saryinya, 吹谷智, 小椋義俊, 林哲也, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2019, 2019

Bifidobacterium animalis subsp.lactisが有するストレス耐性遺伝子の探索
齋藤史, 磯奏子, 尾淵駿, 吹谷智, 横田篤, 山本裕司, 向井孝夫, 日本乳酸菌学会誌, 30, 2, 2019

胆汁酸変換菌の相互作用によるウルソコール酸からデオキシコール酸への新たな変換経路の同定とその変換機構の解明
北川更, TAWTHEP Sarinya, 吹谷智, 小椋義俊, 林哲也, 横田篤, 日本乳酸菌学会誌, 30, 2, 2019

イソフラボン代謝におよぼす胆汁酸の影響
吉岡寛子, 渡辺真通, 難波文男, 鈴木利雄, 吹谷智, 横田篤, 戸田登志也, 日本栄養・食糧学会大会講演要旨集, 73rd, 2019

慢性的な高脂肪食摂取によって腸肝局所で増加する12α水酸化胆汁酸はインスリン感受性を向上させる一方で肝臓脂質蓄積を亢進する
堀将太, 吉次玲香, 菊池慧大, 阿部隆行, 原博, 吹谷智, 横田篤, 石塚敏, 日本栄養・食糧学会大会講演要旨集, 73rd, 2019

Evolutionary traits found in fucosyllactose transporters support symbiosis between bifidobacteria and breast-fed infants
阪中幹祥, 阪中幹祥, 栗原新, 栗原新, 片山高嶺, 片山高嶺, HANSEN Morten Ejby, 後藤愛那, 加藤紀彦, 吉田圭佑, 小田巻俊孝, 杉山友太, 廣瀬潤子, 浦島匡, 清水(肖)金忠, 北岡本光, 吹谷智, 横田篤, LEGGIO Leila Lo, HACHEM Maher Abou, Institute for Fermentation, Osaka. Research Communications, 33, 2019

ピルビン酸カルボキシラーゼ遺伝子の欠失がCorynebacterium glutamicumのリジン生産に与える影響
新森彰信, GUO Zhen, 和田大, 横田篤, 日本生物工学会大会講演要旨集, 71st, 2019

ビフィズス菌の含硫アミノ酸代謝に関与するC-Sリアーゼの解析
黒瀧空, 松本菜々恵, 吹谷智, 横田篤, 和田大, 日本生物工学会大会講演要旨集, 71st, 2019

機能性糖質によるラット盲腸内細菌叢の改変に基づいたデオキシコール酸生成菌と相互作用する腸内細菌種の同定
吹谷智, 佐竹実菜子, 佐藤昌直, 石塚敏, 小椋義俊, 林哲也, 横田篤, 日本乳酸菌学会誌, 30, 2, 2019

Bifidobacterium bifidumの細胞外シアリダーゼの腸粘液との相互作用と糖の資化における役割
西山啓太, 長井暁, 吹谷智, 横田篤, 山本裕司, 向井孝夫, 岡田信彦, 腸内細菌学雑誌, 32, 2, 102, Apr. 2018
Japanese

ビフィズス菌におけるラクト-N-テトラオース輸送体の同定およびビフィズスフローラ形成への関与
阪中 幹祥, 砂川 直輝, 佐藤 真与, 吉田 圭佑, 石田 卓也, 後藤 愛那, 加藤 紀彦, 谷内 寛之, 密山 恵梨, 加藤 久美子, 廣瀬 潤子, 阿部 紘一, 前田 信悟, 杉山 友太, 吹谷 智, 横田 篤, 栗原 新, 荒川 孝俊, 小田巻 俊孝, 清水 金忠, 五十嵐 圭日子, 伏信 進矢, 片山 高嶺, 腸内細菌学雑誌, 32, 2, 101, 101, Apr. 2018
(公財)日本ビフィズス菌センター, Japanese

トランスポゾン変異株集団を用いたビフィズス菌の腸内生存戦略の解明手法の確立
中島森, 久保勇貴, 中川路伸吾, 阪中幹祥, 小椋義俊, 林哲也, 横田篤, 吹谷智, 日本ゲノム微生物学会年会要旨集, 12th, 2018

高脂肪食摂取ラットにおける盲腸内細菌叢変動機構の解析:「胆汁酸仮説」の証明に向けて
横田篤, 渡辺真通, 藤田祐己, 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2018, 2018

ビフィズス菌の腸内生存・定着機構の解明に向けたINSeq法の確立とその評価
中島森, 久保勇貴, 中川路伸吾, 阪中幹祥, 小椋義俊, 吉本真, 吉田圭佑, 清水(肖)金忠, 小田巻俊孝, 林哲也, 横田篤, 吹谷智, 日本乳酸菌学会誌, 29, 2, 2018

ビフィズス菌における低温生残性向上機構の解析
齋藤史, 磯奏子, 小泉亮子, 原翔, 吹谷智, 横田篤, 山本裕司, 向井孝夫, 日本乳酸菌学会誌, 29, 2, 2018

ヒト由来ビフィズス菌Bifidobacterium longum105-Aが通常飼育マウス腸内に定着可能な飼育試験系の確立
嶋田みな, 川瀬陽平, 園山慶, 小椋義俊, 林哲也, 横田篤, 吹谷智, 日本乳酸菌学会誌, 29, 2, 2018

マウス腸内への定着に寄与するビフィズス菌遺伝子の網羅的同定に向けたINSeq法の確立
久保勇貴, 中島森, 中川路伸吾, 阪中幹祥, 小椋義俊, 吉本真, 吉田圭佑, XIAO Jin-Zhong, 小田巻俊孝, 林哲也, 横田篤, 吹谷智, 日本生物工学会大会講演要旨集, 70th, 2018

A unique species-specific structure of lipoteichoic acid common to Lactobacillus gasseri
白石宗, 久富亮佑, 佐藤耶舞羽, 森田直樹, 吹谷智, 佐藤豊孝, 横田篤, 横田伸一, エンドトキシン・自然免疫研究(Web), 21, 2018

エネルギー代謝の改変によるCorynebacterium glutamicumでのピルビン酸生産
片岡尚也, VANGNAI Alisa S., 藥師寿治, 和田大, 横田篤, 松下一信, 日本農芸化学会中四国支部講演会講演要旨集(Web), 50th, 2018

大腸菌のNADH脱水素酵素Iとシトクロムbo₃オキシダーゼ両欠損株におけるNDH-II過剰発現が中枢代謝に与える影響
濱田和宏, 瀬島祐大, 吹谷智, 和田大, 横田篤, 日本生物工学会大会講演要旨集, 70th, 2018

好気代謝の改変によるCorynebacterium glutamicumでの効率的ピルビン酸生産
片岡尚也, 片岡尚也, VANGNAI Alisa S., 藥師寿治, 藥師寿治, 和田大, 横田篤, 松下一信, 松下一信, 日本農芸化学会大会講演要旨集(Web), 2018, 2018

ラットへのコール酸および難消化性オリゴ糖の同時投与による腸内細菌叢改変に基づいたデオキシコール酸生成菌と相互作用する腸内細菌種の探索
佐竹実菜子, 吹谷智, 佐藤昌直, 石塚敏, 小椋義俊, 林哲也, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2018, 2018

ABCトランスポーターを介したビフィズス菌の糖質取込みを包括的に制御するATP加水分解酵素MsiK
阪中幹祥, 前田信悟, 後藤愛那, 村上隆太, 加藤紀彦, 杉山友太, 谷内寛之, 栗原新, 玉置尚徳, 吹谷智, 横田篤, 片山高嶺, 片山高嶺, 日本乳酸菌学会誌, 29, 2, 2018

ビフィズス菌由来ATPase MsiKはABCトランスポーターを介した糖質取込みをグローバルに制御する
前田信悟, 阪中幹祥, 後藤愛那, 村上隆太, 加藤紀彦, 杉山友太, 谷内寛之, 栗原新, 玉置尚徳, 吹谷智, 横田篤, 片山高嶺, 片山高嶺, 日本農芸化学会大会講演要旨集(Web), 2018, 2018

セミナー室 腸内相互作用の理解に基づいた健康の増進・疾患の予防(6)腸内細菌叢の機能理解に向けて : ビフィズス菌における遺伝子操作系の開発
吹谷 智, 横田 篤, 化学と生物 : 日本農芸化学会会誌 : 生命・食・環境, 55, 9, 637, 643, Sep. 2017
日本農芸化学会 ; 1962-, Japanese

大腸菌のNADH脱水素酵素Iとシトクロムbo₃オキシダーゼ両欠損株における異常な糖代謝の解析
瀬島祐大, 紀平千枝, 吹谷智, 和田大, 横田篤, 日本生物工学会大会講演要旨集, 69th, 220, 08 Aug. 2017
Japanese

オキサロ酢酸蓄積型リジン生産Corynebacterium glutamicumの代謝プロファイル解析
佐藤桃梨, 柳瀬真紀, 愛甲徹, 和田大, 横田篤, 日本生物工学会大会講演要旨集, 69th, 218, 08 Aug. 2017
Japanese

ビフィズス菌におけるgalacto-N-biose/lacto-N-biose Iトランスポーターの多様性解析 母乳オリゴ糖lacto-N-tetraoseの取込みに寄与するアミノ酸配列の発見　　　　　　　　　　　　　　　
阪中 幹祥, 石田 卓也, 佐藤 真与, 谷内 寛之, 阿部 紘一, 前田 信悟, 吹谷 智, 横田 篤, 栗原 新, 荒川 孝俊, 五十嵐 圭日子, 伏信 進矢, 片山 高嶺, 日本乳酸菌学会誌, 28, 2, 127, 127, Jun. 2017
日本乳酸菌学会, Japanese

Bifidobacterium longum105‐Aのメチオニン利用に関与する遺伝子の発現解析および遺伝子破壊による検証
和田大, 松本菜々恵, 小松一喜, 阪中幹祥, 松尾実紀, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.4C24a04 (WEB ONLY), 05 Mar. 2017
Japanese

キノール酸化酵素の欠失がCorynebacterium glutamicumのグルタミン酸生産に与える影響
片岡尚也, 片岡尚也, 片岡尚也, 那須裕介, 藥師寿治, 藥師寿治, 藥師寿治, 和田大, 横田篤, 松下一信, 松下一信, 松下一信, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.3C24a01 (WEB ONLY), 05 Mar. 2017
Japanese

Bifidobacterium bifidumにおけるシアリダーゼは宿主との相互作用を促進し糖の資化をサポートする
西山啓太, 山本裕司, 杉山真言, 篠原有香, 浦島匡, 吹谷智, 横田篤, 岡田信彦, 向井孝夫, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.3C18p05 (WEB ONLY), 05 Mar. 2017
Japanese

高脂肪食摂取によるマウス腸内の胆汁酸量の増加が腸内細菌叢構成に与える影響
相磯知里, 松浦史佳, 吹谷智, 渡辺真通, 園山慶, 小椋義俊, 林哲也, 横田篤, 日本乳酸菌学会誌, 28, 2, 2017

コール酸・ケノデオキシコール酸・デオキシコール酸各投与マウスにおける盲腸内胆汁酸組成および菌叢構成の変化
松浦史佳, 相磯知里, 吹谷智, 渡辺真通, 園山慶, 小椋義俊, 林哲也, 横田篤, 日本乳酸菌学会誌, 28, 2, 2017

消化管における生存と定着に寄与するビフィズス菌遺伝子の探索系の構築
中川路伸吾, 中島森, 阪中幹祥, 小椋義俊, 林哲也, 横田篤, 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2017, 2017

二種の腸内細菌の共培養によって明らかになったデオキシコール酸生成の活性化と新たな生成経路
TAWTHEP Sarinya, 鳥居剛史, 山崎真侑, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2017, 2017

消化管における生存と定着に寄与するビフィズス菌遺伝子の網羅的同定に向けたINSeq法の確立
中島森, 中川路伸吾, 阪中幹祥, 小椋義俊, 林哲也, 横田篤, 吹谷智, 日本生物工学会大会講演要旨集, 69th, 2017

Lactobacillus gasseri種に共通するリポテイコ酸の特異的構造
久富亮佑, 白石宗, 佐藤耶舞羽, 森田直樹, 吹谷智, 佐藤豊孝, 横田篤, 横田伸一, 日本細菌学雑誌(Web), 72, 1, 2017

コリネ型細菌のグルタミン酸発酵におけるキノール酸化酵素の役割
那須裕介, 片岡尚也, 片岡尚也, 片岡尚也, 薬師寿治, 薬師寿治, 薬師寿治, 和田大, 横田篤, 松下一信, 松下一信, 松下一信, 日本農芸化学会中四国支部講演会講演要旨集(Web), 47th, ROMBUNNO.F‐2 (WEB ONLY), 2017
Japanese

Characteristics of lactic acid bacteria from the view of the structural diversity of lipoteichoic acids
白石宗, 横田伸一, 吹谷智, 横田篤, JATAFFジャーナル, 5, 3, 26, 32, 2017
農林水産・食品産業技術振興協会, Japanese, Introduction scientific journal

中枢代謝活性化によるアミノ酸発酵の効率化
和田大, 横田篤, 電子情報通信学会大会講演論文集(CD-ROM), 2016, ROMBUNNO.BI‐8‐2, 06 Sep. 2016
Japanese

D‐erythro‐3‐ヒドロキシアスパラギン酸デヒドラターゼの機能解析および光学分割への応用
柴野可菜, 長野弘幸, 横田篤, 和田大, 日本生物工学会大会講演要旨集, 68th, 290, 25 Aug. 2016
Japanese

リンゴ酸デヒドロゲナーゼ欠失がCorynebacterium glutamicumのリジン生産に与える影響
佐藤桃梨, 柳瀬真紀, 愛甲徹, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 2F151 (WEB ONLY), 05 Mar. 2016
Japanese

大腸菌の1,3‐ブタンジオール高生産に向けた代謝改変
片岡尚也, VANGNAI Alisa S, 藥師寿治, 加藤純一, 和田大, 横田篤, 松下一信, 日本農芸化学会大会講演要旨集(Web), 2016, 3A044 (WEB ONLY), 05 Mar. 2016
Japanese

Bifidobacterium longum105‐Aのメチオニン利用性に関与するS‐アデノシルメチオニン回路の解析
松本菜々恵, 小松一喜, 阪中幹祥, 鈴木梓, 吹谷智, 横田篤, 和田大, 日本農芸化学会大会講演要旨集(Web), 2016, 2F138 (WEB ONLY), 05 Mar. 2016
Japanese

クエン酸シンターゼの活性低下がCorynebacterium glutamicumのリジン生産に与える影響
愛甲徹, 柳瀬真紀, 佐藤桃梨, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 2F150 (WEB ONLY), 05 Mar. 2016
Japanese

呼吸鎖改変大腸菌ΔNDH‐IΔCyt boの酸素制限による糖代謝の変化
杉本諒, 加藤竜一, 荒井博紀, 紀平知枝, 吹谷智, 和田大, 片岡尚也, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 3F144 (WEB ONLY), 05 Mar. 2016
Japanese

高脂肪食摂取ラットの盲腸内菌叢の変化における胆汁酸の寄与:「胆汁酸仮説」の証明にむけて
渡辺真通, 萩尾真人, 李慈英, 小椋義俊, 林哲也, 清水英寿, 石塚敏, 吹谷智, 横田篤, 腸内細菌学雑誌, 30, 2, 2016

高脂肪食摂取ラットの盲腸内菌叢の変化における腸内細菌の胆汁酸耐性の寄与
渡辺真通, 萩尾真人, LEE Ja-Young, 小椋義俊, 林哲也, 清水英寿, 石塚敏, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 2016

対数期におけるLactobacillus gasseri JCM1131^Tのリポテイコ酸の局在
白石宗, 横田伸一, 佐藤耶舞羽, 伊藤利章, 吹谷智, 山本聡, 小笠原徳子, 佐藤豊孝, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 2016

腸内生存に寄与するビフィズス菌遺伝子の探索系の構築
中川路伸吾, 阪中幹祥, 横田篤, 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2016, 2016

R-IVET法によるマウス消化管特異的に発現するビフィズス菌遺伝子の同定
石神夏実, 河口礼佳, 平等清夏, 阪中幹祥, 横田篤, 吹谷智, 日本生物工学会大会講演要旨集, 68th, 2016

マウス消化管内で特異的に発現するビフィズス菌遺伝子の同定法の確立
河口礼佳, 平等清夏, 石神夏実, 阪中幹祥, 横田篤, 吹谷智, 日本ゲノム微生物学会年会要旨集, 10th, 2016

トランスポゾン変異導入系を用いたビフィズス菌の腸内生存に寄与する遺伝子の探索系の構築
中川路伸吾, 阪中幹祥, 横田篤, 吹谷智, 日本ゲノム微生物学会年会要旨集, 10th, 2016

食品タンパク質由来腸内細菌代謝産物スカトールが糞中胆汁酸組成および肝臓に与える影響
清水英寿, 清水英寿, 田中愛建, 野勢琢馬, 吹谷智, 横田篤, 石塚敏, 日本栄養・食糧学会中国・四国支部大会講演要旨集, 49th, 2016

大腸菌でのpdhR欠損およびNADPH供給の強化は1,3‐ブタンジオール生産を向上させる
片岡尚也, VANGNAI Alisa S, 藥師寿治, 加藤純一, 和田大, 横田篤, 松下一信, 日本農芸化学会中四国支部講演会講演要旨集(Web), 44th, F-7 (WEB ONLY), 2016
Japanese

Lactobacillus gasseri JCM 1131Tの対数期におけるリポテイコ酸の局在：Membrane vesicle表層に露出されるリポテイコ酸　　　　　　　　　　　　　　　
白石宗, 佐藤耶舞羽, 横田伸一, 伊藤利章, 吹谷智, 横田篤, エンドトキシン・自然免疫研究19－新たなる基礎と臨床の架け橋－, 19, 15, 17, 2016
Japanese, Introduction commerce magazine

Corynebacterium glutamicumピルビン酸キナーゼ欠失株における代謝変化とそのメカニズム
横田篤, 澤田和典, 萩原拓也, 今井恵太, 禅院進, 和田大, 日本生物工学会大会講演要旨集, 67th, 116, 25 Sep. 2015
Japanese

大腸菌のピルビン酸脱水素酵素複合体制御因子PdhR欠損変異株での1,3‐ブタンジオール生産
片岡尚也, VANGNAI Alisa S, 藥師寿治, 加藤純一, 和田大, 横田篤, 松下一信, 日本生物工学会大会講演要旨集, 67th, 115, 25 Sep. 2015
Japanese

Bifidobacterium longum 105‐Aのメチオニン利用性に関与するC‐Sリアーゼの解析
松本菜々恵, 鈴木梓, 小松一喜, 吹谷智, 横田篤, 和田大, 日本農芸化学会北海道支部講演会講演要旨, 2015, 17, 08 Aug. 2015
Japanese

ピルビン酸キナーゼ欠失がCorynebacterium glutamicumのリジン生産に与える影響
横田篤, 柳瀬真紀, 愛甲徹, 和田大, 日本農芸化学会大会講演要旨集(Web), 2015, 2A37P10 (WEB ONLY), 05 Mar. 2015
Japanese

Bifidobacterium longum105‐Aのメチオニン利用においてS‐リボシルホモシステインリアーゼが重要な役割を果たす
和田大, 小松一喜, 松本菜々恵, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2015, 2A37P11 (WEB ONLY), 05 Mar. 2015
Japanese

高脂肪食摂取がラット盲腸内容物の胆汁酸分泌および菌叢変化に与える影響
渡辺真通, 萩尾真人, 藤田祐己, LEE Ja-Young, 馬場菜那子, 小椋義俊, 小椋義俊, 林哲也, 林哲也, 清水英寿, 石塚敏, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2015, 2015

INSeq法を用いた腸内生存に寄与するビフィズス菌遺伝子の探索系の構築
中川路伸吾, 阪中幹祥, 横田篤, 吹谷智, 日本乳酸菌学会誌, 26, 2, 2015

コール酸長期摂取ラットにおける腸内細菌叢変動を介した非肥満性脂肪肝の発症
横田篤, 李慈英, 清水英寿, 萩尾真人, 吹谷智, 大岡唯祐, 小椋義俊, 林哲也, 石塚敏, 日本未病システム学会学術総会抄録集, 22nd, 2015

IS3 family挿入配列ISBlo11を用いたビフィズス菌のトランスポゾン変異導入系の確立
阪中幹祥, 横田篤, 吹谷智, 日本ゲノム微生物学会年会要旨集, 9th, 2015

Bifidobacterium breve JCM 1192^Tを指標菌としたラット腸内に存在する胆汁酸分子種の細胞毒性評価
渡辺真通, 吹谷智, 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2015, 2015

マウス腸内で特異的に発現するビフィズス菌遺伝子の探索
河口礼佳, 石神夏実, 平等清夏, 阪中幹祥, 横田篤, 吹谷智, 日本農芸化学会北海道支部講演会講演要旨, 2015, 2015

ビフィズス菌におけるシアリダーゼのムチン付着因子としての新たな役割
西山啓太, 西山啓太, 篠原有香, 浦島匡, 吹谷智, 横田篤, 山本裕司, 向井孝夫, 日本乳酸菌学会誌, 26, 2, 2015

腸内乳酸菌 Lactobacillus gasseri JCM 1131T のリポテイコ酸に認められた新奇構造　　　　　　　　　　　　　　　
白石宗, 横田伸一, 吹谷智, 横田篤, エンドトキシン・自然免疫研究18－自然免疫における生体防御ペプチドの多様性－, 18, 79, 81, 2015
Japanese, Introduction other

Delftia sp. HT23株由来D‐threo‐3‐ヒドロキシアスパラギン酸デヒドラターゼへの低活性変異導入による酵素‐基質複合体構造解析
松本悠, 安武義晃, 田村具博, 横田篤, 和田大, 日本農芸化学会北海道支部講演会講演要旨, 2014, 29, 22 Sep. 2014
Japanese

ビフィズス菌のメチオニン資化性に関与するS‐アデノシルメチオニン回路の解析
小松一喜, 鈴木梓, 阪中幹祥, 吹谷智, 横田篤, 和田大, 日本生物工学会大会講演要旨集, 66th, 46, 05 Aug. 2014
Japanese

代謝トグルスイッチを用いた細胞内代謝流束制御によるIPA生産効率の向上
相馬悠希, 鶴野圭悟, 和田大, 横田篤, 花井泰三, 日本生物工学会大会講演要旨集, 66th, 246, 05 Aug. 2014
Japanese

大腸菌によるものつくりをめざした中枢代謝と電子伝達系のエンジニアリング
横田篤, 和田大, 日本生物工学会大会講演要旨集, 66th, 7, 05 Aug. 2014
Japanese

1P-115 Effects of respiratory-chain-enzyme defective mutations on central carbon metabolism in Escherichia coli
KATOH Ryuichi, ARAI Hironori, KIHIRA Chie, FUKIYA Satoru, WADA Masaru, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 66, 46, 46, 05 Aug. 2014
The Society for Biotechnology, Japan, Japanese

ビフィズス菌の逆流硫黄経路の同定と活性染色法によるC‐Sリアーゼの解析
鈴木梓, 和田大, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2014, 3A15P17 (WEB ONLY), 05 Mar. 2014
Japanese

Lactobacillus gasseriにおける生育段階によるリポテイコ酸の局在変化
白石宗, 横田伸一, 佐藤耶舞羽, 伊藤利章, 吹谷智, 横田篤, 日本乳酸菌学会誌, 25, 2, 2014

微生物のストレス応答 腸管内での胆汁酸ストレスと腸内乳酸菌の生残戦略
白石宗, 横田伸一, 吹谷智, 横田篤, 日本乳酸菌学会誌, 25, 1, 2014

大腸菌低活性型のビフィズス菌プロモーターの同定およびトランスポゾン変異導入系への応用
阪中幹祥, 玉井早紀, 平山洋佑, 横田篤, 吹谷智, 日本乳酸菌学会誌, 25, 2, 2014

大腸菌およびBifidobacterium longumにおけるビフィズス菌プロモーターの機能解析
阪中幹祥, 玉井早紀, 平山洋佑, 横田篤, 吹谷智, 日本ゲノム微生物学会年会要旨集, 8th, 2014

コール酸経口負荷による肝脂質蓄積
辻美咲, 吉次玲香, 菊地慧大, 野勢琢馬, 清水英寿, 李慈英, 馬場菜那子, 萩尾真人, 吹谷智, 横田篤, 原博, 石塚敏, 肥満研究, 20, Supplement, 2014

コール酸長期摂取ラットにおける糞便中胆汁酸組成の変化と腸内細菌叢との関係
馬場菜那子, 萩尾真人, LEE Ja-Young, 吹谷智, 小椋義俊, 小椋義俊, 清水英寿, 大岡唯祐, 林哲也, 林哲也, 石塚敏, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2014, 2014

胆汁酸と腸内フローラ:メタボリックシンドローム発症との関連性
横田篤, 吹谷智, ISLAM K.B.M. Saiful, 大岡唯祐, 小椋義俊, 小椋義俊, 林哲也, 林哲也, 萩尾真人, 石塚敏, 日本農芸化学会大会講演要旨集(Web), 2014, 2014

消化管内で発現するビフィズス菌遺伝子の探索系の構築
河口礼佳, 平等清夏, 阪中幹祥, 平山洋佑, 横田篤, 吹谷智, 日本乳酸菌学会誌, 25, 2, 2014

消化管内特異的に発現するビフィズス菌遺伝子の探索系の構築
平等清夏, 河口礼佳, 阪中幹祥, 平山洋佑, 横田篤, 吹谷智, 日本ゲノム微生物学会年会要旨集, 8th, 2014

ビフィズス菌由来シアリダーゼのムチン付着因子としての評価
西山啓太, 吹谷智, 横田篤, 山本裕司, 向井孝夫, 日本畜産学会大会講演要旨, 118th, 2014

Skatoleの経口負荷が肝Cyp7a1発現と二次胆汁酸排泄の亢進を誘導する
野勢琢馬, 清水英寿, 萩尾真人, 吹谷智, 横田篤, 原博, 石塚敏, 肥満研究, 20, Supplement, 2014

腸内細菌代謝物スカトールは肝臓でのCyp7a1の発現と糞中二次胆汁酸増加を誘導する
野勢琢馬, 清水英寿, 萩尾真人, 吹谷智, 横田篤, 原博, 石塚敏, 日本分子生物学会年会プログラム・要旨集(Web), 37th, 2014

2P-023 Development of a screening system for Bifidobacterium genes specifically expressed in vivo
Koguchi Hiroka, Hirato Sayaka, Sakanaka Mikiyasu, Yokota Atsushi, Fukiya Satoru, 日本生物工学会大会講演要旨集, 66, 112, 112, 2014
日本生物工学会, Japanese

1P-117 Effect of fumarate on the cholic acid metabolism in Clostridium hiranonis
Torii Tsuyoshi, Honda Nozomi, Hagio Masahito, Fukiya Satoru, Yokota Atsushi, 日本生物工学会大会講演要旨集, 66, 46, 46, 2014
日本生物工学会, Japanese

ビフィズス菌のシアリダーゼは付着因子として機能する
西山啓太, 吹谷智, 横田篤, 山本裕司, 向井孝夫, ミルクサイエンス, 63, 2, 2014

Characterization of a Lactobacillus gasseri JCM 1131(T) Lipoteichoic Acid with a Novel Glycolipid Anchor Structure (vol 79, pg 3315, 2013)
Tsukasa Shiraishi, Shin-ichi Yokota, Naoki Morita, Satoru Fukiya, Satoru Tomita, Naoto Tanaka, Sanae Okada, Atsushi Yokota, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79, 24, 7931, 7931, Dec. 2013
English, Others

Corynebacterium glutamicumのリンゴ酸‐キノン酸化還元酵素は中枢代謝におけるストレス応答時の制御点として機能する
萩原拓也, 澤田和典, 和田大, 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2013, 16, 27 Nov. 2013
Japanese

1P-114 Effects of pyruvate kinase gene deletion and phosphoenolpyruvate carboxylase feedback resistance on lysine productivity in Corynebacterium glutamicum
Yanase Masaki, Hagiwara Takuya, Ogura Kotaro, Wada Masaru, Yokota Atsushi, 日本生物工学会大会講演要旨集, 65, 25 Aug. 2013
The Society for Biotechnology, Japan, Japanese

2P-093 Effects of pdhR-deleted mutation on glucose metabolism in Escherichia coli
Iwabu Yuki, Kihira Chie, Maeda Soya, Fukiya Satoru, Wada Masaru, Yokota Atsushi, 日本生物工学会大会講演要旨集, 65, 127, 127, 25 Aug. 2013
The Society for Biotechnology, Japan, Japanese

Oral administration of cholic acid modulates the gut microbiota and epithelial proliferation
Satoshi Ishizuka, Manami Takatsuki, Maiko Shiwaku, Masahito Hagio, Reika Yoshitsugu, Keidai Kikuchi, Nobuyuki Fujii, Ga-Hyun Joe, Hidehisa Shimizu, Hiroshi Hara, Satoru Fukiya, Atsushi Yokota, FASEB JOURNAL, 27, Apr. 2013
English, Summary international conference

微生物の共存・共生と相互作用 遺伝子変異導入法の確立により見えてきたビフィズス菌の腸管定着因子
阪中幹祥, 吹谷智, 横田篤, 日本乳酸菌学会誌, 24, 1, 36, 15 Mar. 2013
Japanese

新規D‐erythro‐3‐hydroxyaspartate分解酵素の探索及び一次構造解析
和田大, 長野弘幸, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2013, 2C15P05 (WEB ONLY), 05 Mar. 2013
Japanese

D‐threo‐3‐ヒドロキシアスパラギン酸デヒドラターゼの立体構造解析
松本悠, 安武義晃, 武田由希, 田村具博, 横田篤, 和田大, 日本農芸化学会大会講演要旨集(Web), 2013, 2C15A15 (WEB ONLY), 05 Mar. 2013
Japanese

乳酸菌のリポテイコ酸のイムノブロットによる検出方法の確立
佐藤耶舞羽, 白石宗, 横田伸一, 吹谷智, 横田篤, 日本乳酸菌学会誌, 24, 2, 2013

二重相同組換え法を用いたBifidobacterium longumの染色体上遺伝子への欠失変異導入技術の開発
吹谷智, 平山洋佑, 阪中幹祥, 福間英訓, 村山寛樹, 加納康正, 横田篤, 日本ゲノム微生物学会年会要旨集, 7th, 2013

腸内特異的に発現するビフィズス菌遺伝子の探索系の構築
平等清夏, 河口礼佳, 阪中幹祥, 平山洋佑, 吹谷智, 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2013, 2013

異種遺伝子発現への応用を目的としたビフィズス菌のプロモーターの評価
玉井早紀, 阪中幹祥, 平山洋佑, 吹谷智, 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2013, 2013

Lactobacillus gasseri JCM1131^Tに見出された新規なリポテイコ酸の構造
白石宗, 横田伸一, 森田直樹, 吹谷智, 冨田理, 田中尚人, 岡田早苗, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2013, 2013

プロバイオティクス機能の分子機構解明に向けたビフィズス菌の遺伝子欠損導入系の開発
吹谷智, 平山洋佑, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2013, 2013

Basic research on the enhancement of central metabolism in bacteria producing useful metabolites
Yokota Atsushi, 生物工学会誌 : seibutsu-kogaku kaishi, 91, 6, 294, 300, 2013
日本生物工学会, Japanese

Corynebacterium glutamicumのオキサロ酢酸供給強化に向けた代謝改変
萩原拓也, 下野雄太, 澤田和典, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2013, 2013

COMPARISON OF FERMENTATION LEVELS IN LARGE INTESTINE OF RATS FED DIFFERENT CARBOHYDRATE-SOURCE DIETS BY ANALYZING FECES USING LC/MS
M. Hagio, M. Matsumoto, A. Shinoki, A. Yokota, S. Ishizuka, ANNALS OF NUTRITION AND METABOLISM, 63, 362, 363, 2013
English, Summary international conference

Bifidobacterium longum105‐Aにおけるシステイン/メチオニン代謝の解析
鈴木梓, 吹谷智, 横田篤, 和田大, 日本農芸化学会北海道支部講演会講演要旨, 2012, 20, 01 Nov. 2012
Japanese

大腸菌とコリネ型グルタミン酸生産菌のエネルギー代謝工学(代謝工学,<特集>バイオ技術10年の軌跡,創立90周年記念特別企画)
横田 篤, 和田 大, 生物工学会誌 : seibutsu-kogaku kaishi, 90, 10, 621, 622, 25 Oct. 2012
公益社団法人日本生物工学会, Japanese

呼吸鎖酵素およびPdhRの欠失による大腸菌の糖代謝活性の変化
荒井博紀, 紀平知枝, 前田壮矢, 吹谷智, 和田大, 横田篤, 日本生物工学会大会講演要旨集, 64th, 204, 25 Sep. 2012
Japanese

4Dp01 Metabolic engineering for enhanced supply of oxaloacetate in Corynebacterium glutamicum
Ogura Kotaro, Sawada Kazunori, Hagiwara Takuya, Yanase Masaki, Wada Masaru, Yokota Atsushi, 日本生物工学会大会講演要旨集, 64, 25 Sep. 2012
The Society for Biotechnology, Japan, Japanese

2Cp18 Diversity of 3-hydroxyaspartate degrading enzyme found in soilbacteria
Nagano Hiroyuki, Yokota Atsushi, Wada Masaru, 日本生物工学会大会講演要旨集, 64, 43, 43, 25 Sep. 2012
The Society for Biotechnology, Japan, Japanese

新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53由来の7β‐hydroxysteroid dehydrogenaseの発現と機能解析
李慈英, 吹谷智, 和田大, 横田篤, 日本乳酸菌学会誌, 23, 2, 124, 01 Jul. 2012
Japanese

Corynebacterium glutamicumのピルビン酸キナーゼ欠失およびクエン酸シンターゼ変異がアミノ酸生産に与える影響
澤田和典, 下野雄太, 小倉紘太郎, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C20P17 (WEB ONLY), 05 Mar. 2012
Japanese

Corynebacterium glutamicumのピルビン酸キナーゼ欠失およびアナプレロティック経路変異がアミノ酸生産に与える影響
下野雄太, 澤田和典, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C20P18 (WEB ONLY), 05 Mar. 2012
Japanese

pdhR欠失変異が大腸菌の糖代謝に与える影響
前田壮矢, 紀平知枝, 清水久美子, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C20P14 (WEB ONLY), 05 Mar. 2012
Japanese

NADH dehydrogenase II過剰発現による大腸菌の糖代謝特性の変動
紀平知枝, 林由香里, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C20P15 (WEB ONLY), 05 Mar. 2012
Japanese

酵素法による光学活性3‐ヒドロキシアスパラギン酸異性体の調製
武田由希, 田中修, 横田篤, 和田大, 日本農芸化学会大会講演要旨集(Web), 2012, 3C23P11 (WEB ONLY), 05 Mar. 2012
Japanese

新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53由来の7β‐hydroxysteroid dehydrogenaseの発現と機能解析
LEE JaYoung, 荒井尚志, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C09A11 (WEB ONLY), 05 Mar. 2012
Japanese

腸内乳酸菌Lactobacillus gasseri JCM1131^Tのリポテイコ酸の構造とコール酸適応への関与
白石宗, 冨田理, 田中尚人, 吹谷智, 森田直樹, 横田伸一, 岡田早苗, 横田篤, 日本乳酸菌学会誌, 23, 2, 2012

ビフィズス菌由来挿入配列TLS143の転移機能の解析
阪中幹祥, 阿部光紗, 平山洋佑, 吹谷智, 加納康正, 横田篤, 腸内細菌学雑誌, 26, 2, 2012

ランダム変異導入法への応用を目指したビフィズス菌由来挿入配列TLS143の機能解析
阪中幹祥, 阿部光紗, 平山洋佑, 吹谷智, 加納康正, 横田篤, 日本ゲノム微生物学会年会要旨集, 6th, 2012

新規大腸菌-ビフィズス菌シャトルベクターの構築
小野寺愛, 平山洋佑, 阪中幹祥, 吹谷智, 片山高嶺, 加納康正, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 2012

Lactobacillus gasseri JCM1131^Tのリポテイコ酸:胆汁酸適応への関与
白石宗, 冨田理, 田中尚人, 吹谷智, 森田直樹, 横田伸一, 岡田早苗, 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 2012

4Fp27 Bile acid is a host factor that regulates the composition of the cecal microbiota in rats
Fukiya Satoru, Islam K.B.M. Saiful, Hagio Masahito, Fujii Nobuyuki, Ishizuka Satoshi, Ooka Tadasuke, Ogura Yoshitoshi, Hayashi Tetsuya, Yokota Atsushi, 日本生物工学会大会講演要旨集, 64, 219, 219, 2012
日本生物工学会, Japanese

4Dp11 Effect of fumarate on bile acid transformation by intestinal bacteria
Honda Nozomi, Chang Hsin-Yu, Fukiya Satoru, Ishizuka Satoshi, Yokota Atsushi, 日本生物工学会大会講演要旨集, 64, 208, 208, 2012
日本生物工学会, Japanese

大腸菌の呼吸鎖改変による糖代謝変化に関する研究
荒井博紀, 紀平知枝, 吹谷智, 和田大, 横田篤, 日本生体エネルギー研究会討論会講演要旨集, 38th, 21, 22, 2012
Japanese

2Ha09 Effectiveness of pdhR deletion in glucose metabolism of Escherichia coli
MAEDA Soya, KIHIRA Chie, SHIMIZU Kumiko, FUKIYA Satoru, WADA Masaru, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 0, 63, 168, 168, 25 Aug. 2011
公益社団法人日本生物工学会, Japanese

Delftia sp.HT23株由来のD‐スレオ‐3‐ヒドロキシアスパラギン酸デヒドラターゼの機能解析と発現量増大
武田由希, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2011, 197, 05 Mar. 2011
Japanese

大腸菌のpdhR欠失変異株の培養特性解析
前田壮矢, 紀平知枝, 清水久美子, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2011, 90, 05 Mar. 2011
Japanese

酵素的不斉還元反応を用いた光学活性フェニルセリンの合成
井村友哉, 川端潤, 安田磨理, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2011, 118, 05 Mar. 2011
Japanese

新規ウルソデオキシコール酸生成腸内細菌の反応特性の解析
荒井尚志, 李慈英, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2011, 274, 05 Mar. 2011
Japanese

呼吸鎖欠損が大腸菌の糖代謝に与える影響
紀平知枝, 前田壮矢, 吹谷智, 和田大, 松下一信, 横田篤, 日本農芸化学会大会講演要旨集, 2011, 90, 05 Mar. 2011
Japanese

コール酸添加食摂取がラット盲腸内の細菌叢及び胆汁酸代謝に与える影響
SAIFUL Islam K. B. M., 吹谷智, 萩尾真人, 石塚敏, 横田篤, 日本農芸化学会大会講演要旨集, 2011, 2011

Lactobacillus gasseri JCM1131における推定カルジオリピンシンターゼ遺伝子欠失がコール酸適応現象に与える影響
戸部はるひ, 澤田真理子, 加藤慎二, 森田直樹, 佐々木泰子, 扇谷悟, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集, 2011, 2011

ビフィズス菌の挿入配列を利用したランダム変異導入系の確立
阿部光紗, 阪中幹祥, 小林諒子, 平山洋佑, 吹谷智, 加納康正, 横田篤, 日本農芸化学会大会講演要旨集, 2011, 2011

Bifidobacterium longum105-Aのα-ガラクトシダーゼ遺伝子破壊株の性状解析とレポーターアッセイ系への応用
平山洋佑, 福間英訓, 村山寛樹, 吹谷智, 加納康正, 横田篤, 日本乳酸菌学会誌, 22, 2, 2011

Bifidobacterium longum105-A由来挿入配列TLS143の挿入特異性および転移活性の解析
阪中幹祥, 阿部光紗, 平山洋佑, 吹谷智, 加納康正, 横田篤, 日本乳酸菌学会誌, 22, 2, 2011

NADH dehydrogenase IIの過剰発現による大腸菌の糖代謝活性の増大および呼吸鎖欠損株への導入による糖代謝改善効果
紀平知枝, 林由香里, 吹谷智, 和田大, 横田篤, 日本生体エネルギー研究会討論会講演要旨集, 37th, 96, 97, 2011
Japanese

新規D‐erythro‐3‐ヒドロキシアスパラギン酸変換酵素の探索
長野弘幸, 横田篤, 和田大, 日本農芸化学会北海道支部講演会講演要旨, 2011, 7, 2011
Japanese

2P-1109 Optimization of culture conditions for physiological analysis of fermentation in Escherichia coli
Kihira Chie, Maeda Soya, Fukiya Satoru, Wada Masaru, Yokota Atsushi, 日本生物工学会大会講演要旨集, 22, 0, 25 Sep. 2010
公益社団法人日本生物工学会, Japanese

Bile acid stress response in lactic acid bacteria and bifidobacteria
YOKOTA Atsushi, 日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria, 21, 2, 87, 94, 01 Jul. 2010
Japanese

呼吸鎖欠損変異による大腸菌の糖代謝特性の変化
紀平知枝, 吹谷智, 和田大, 松下一信, 横田篤, 日本農芸化学会大会講演要旨集, 2010, 205, 05 Mar. 2010
Japanese

H⁺‐ATPase欠損がCorynebacterium glutamicumの呼吸関連酵素活性へ与える影響
嘉藤由衣, 今井恵太, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2010, 206, 05 Mar. 2010
Japanese

Delftia sp.HT23株に見出されたD‐threo‐3‐ヒドロキシアスパラギン酸デヒドラターゼの機能解析
武田由希, 井村友哉, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2010, 19, 05 Mar. 2010
Japanese

Corynebacterium glutamicumピルビン酸キナーゼ欠失株における菌体収率の向上
澤田和典, 今井恵太, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2010, 206, 05 Mar. 2010
Japanese

嫌気呼吸の促進によるラット腸管における発癌二次胆汁酸生成抑制の試み
張シンユ, 萩尾真人, 吹谷智, 石塚敏, 横田篤, 日本農芸化学会大会講演要旨集, 2010, 2010

16S rRNA遺伝子クローンライブラリー法による胆汁酸添加食摂取ラット腸内細菌叢の解析
SAIFUL ISLAM K. B. M., 萩尾真人, 石塚敏, 吹谷智, 横田篤, 腸内細菌学雑誌, 24, 2, 2010

腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構と細胞膜脂質組成変化の関連性
加藤慎二, 森田直樹, 佐々木泰子, 扇谷悟, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集, 2010, 2010

胆汁酸代謝を介した生理作用評価のための実験系構築の試み
藤井暢之, 萩尾真人, 安藤翠, ISLAM K. B. M. Saiful, 吹谷智, 横田篤, 原博, 石塚敏, 日本食物繊維学会誌, 14, 2, 2010

Bifidobacterium longum105-A株における染色体遺伝子破壊技術の構築
平山洋佑, 福間英訓, 村山寛樹, 吹谷智, 加納康正, 横田篤, 日本農芸化学会大会講演要旨集, 2010, 2010

Bifidobacterium longum105-A株における染色体上の標的遺伝子破壊技術の構築
平山洋佑, 福間英訓, 村山寛樹, 吹谷智, 加納康正, 横田篤, 日本乳酸菌学会誌, 21, 2, 2010

Corynebacterium glutamicum ATCC 13032のピルビン酸キナーゼ欠失株における代謝変化
澤田和典, 和田大, 横田篤, 日本生物工学会シンポジウム講演要旨集, 2010, 20, 2010
Japanese

1Dp18 Enzymatic preparation of 3-hydroxyaspartate isomers
WADA Masaru, TANAKA Shuu, SHINOHARA Shigeki, TAKEDA Yuki, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 21, 0, 25 Aug. 2009
公益社団法人日本生物工学会, Japanese

2Fa15 Enhancement of glucose metabolism by pdhR deletion in Escherichia coli
Kihira Chie, Shimizu Kumiko, Fukiya Satoru, Wada Masaru, Yokota Atsushi, 日本生物工学会大会講演要旨集, 21, 0, 77, 77, 25 Aug. 2009
公益社団法人日本生物工学会, Japanese

Corynebacterium glutamicumのピルビン酸キナーゼ欠失株における代謝変化
澤田和典, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2009, 260, 05 Mar. 2009
Japanese

新規二次胆汁酸生成菌Bacteroides intestinalis AM‐1の7‐oxo胆汁酸の生成特性について
川嶋紘子, 荒田美紀, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2009, 124, 05 Mar. 2009
Japanese

Pseudomonas sp.T62由来L‐threo‐3‐hydroxyaspartate dehydrataseへの変異導入による機能改変
和田大, 村上知子, 横田篤, 日本農芸化学会大会講演要旨集, 2009, 37, 05 Mar. 2009
Japanese

新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53の分離および本株の胆汁酸変換反応の解析
中村勇介, 荒田美紀, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2009, 123, 05 Mar. 2009
Japanese

呼吸鎖欠損変異による大腸菌の糖代謝活性の向上
紀平知枝, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2009, 261, 05 Mar. 2009
Japanese

Corynebacterium glutamicumのH⁺‐ATPase欠損による呼吸関連酵素活性の変化
嘉藤由衣, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2009, 261, 05 Mar. 2009
Japanese

腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構の解明
加藤慎二, 澤田真理子, 松原裕樹, 森田直樹, 佐々木泰子, 扇谷悟, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集, 2009, 2009

腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構と細胞膜脂質組成変化の関連性
加藤慎二, 澤田真理子, 松原裕樹, 森田直樹, 佐々木泰子, 扇谷悟, 吹谷智, 横田篤, 日本乳酸菌学会誌, 20, 2, 2009

Corynebacterium glutamicumのピルビン酸キナーゼ欠失株の代謝変化とその利用
今井恵太, 澤田和典, 和田大, 横田篤, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2009, 15, 2009
Japanese

呼吸鎖欠損変異が大腸菌の糖代謝に与える影響
紀平知枝, 吹谷智, 和田大, 横田篤, 日本生体エネルギー研究会討論会講演要旨集, 35th, 33, 34, 2009
Japanese

急展開を見せる腸内細菌研究 : 大規模シークエンス解析により明らかになってきた腸内細菌叢の構造と機能
吹谷 智, 横田 篤, 化学と生物, 47, 1, 11, 13, 01 Jan. 2009
社団法人 日本農芸化学会, Japanese

Defective F1-ATPase　　　　　　　　　　　　　　　
John Wiley & Sons, Inc. USA, 2009

2Fa10 Application of D-threo-3-dehydratase from Delftia sp. HT23
WADA Masaru, SHINOHARA Shigeki, MAEDA TAKAYUKI, YOKOTA ATSUSHI, 日本生物工学会大会講演要旨集, 20, 0, 160, 160, 11 Jul. 2008
公益社団法人日本生物工学会, Japanese

3S2a03 Enhancement of central metabolism by H^+-ATPase defect : comparison between Escherichia coli and Corynebacterium glutamicum
YOKOTA Atsushi, WADA Masaru, 日本生物工学会大会講演要旨集, 20, 0, 23, 23, 11 Jul. 2008
公益社団法人日本生物工学会, Japanese

Pseudomonas sp.T62由来のL‐threo‐3‐ヒドロキシアスパラギン酸デヒドラターゼの機能解析
村上知子, 前田隆行, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2008, 26, 05 Mar. 2008
Japanese

Delftia sp.HT23由来のD‐threo‐3‐ヒドロキシアスパラギン酸デヒドラターゼのクローニングと発現
前田隆行, 村上知子, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2008, 26, 05 Mar. 2008
Japanese

Corynebacterium glutamicumのピルビン酸キナーゼ欠損株の解析
禅院進, 嘉藤由衣, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2008, 104, 05 Mar. 2008
Japanese

呼吸鎖欠損変異が大腸菌の糖代謝活性に与える影響
紀平知枝, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2008, 86, 05 Mar. 2008
Japanese

腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構の解明
松原裕樹, 森田直樹, 佐々木泰子, 扇谷悟, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008

新たな腸内細菌叢解析手法としてのFISH-フローサイトメトリー法
吹谷智, 横田篤, 生化学, 80, 5, 2008

Bifidobacterium longumからの転位活性を持つ挿入配列の単離
吹谷智, 小林諒子, 加納康正, 横田篤, 日本乳酸菌学会誌, 19, 2, 2008

ヒト腸内フローラの迅速・網羅的解析法の開発
辻浩和, 松田一乗, 朝原崇, 松本一政, 松木隆広, 野本康二, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008

ビフィズス菌からの転位能をもつ挿入配列の単離と同定
小林諒子, 吹谷智, 加納康正, 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008

プロテオーム解析によるLactobacillus gasseri JCM 1131^Tのコール酸適応機構の解明
平野友美, 加藤慎二, 佐々木泰子, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008

Oenococcus oeni由来プラスミドpOM1に存在するORF3は酸耐性に関与するレギュレーター様タンパク質をコードする
小田美也子, 澤渡優喜, 吹谷智, 横田篤, 池田隆幸, 日本農芸化学会大会講演要旨集, 2008, 2008

01-014 Exploration of novel lipolytic gene by metagenomic approach(Soil ecosystem)
Suzuki Aya, Narihiro Takashi, Kimura Nobutada, Yumoto Isao, Yokota Atsushi, Kamagata Yoichi, 日本微生物生態学会講演要旨集, 24, 65, 65, 2008
日本微生物生態学会, Japanese

S-B1 Monitoring of population dynamics of bifidobacteria in human intestine by FISH-flow cytometry
横田 篤, 吹谷 智, 日本微生物生態学会講演要旨集, 24, 183, 183, 2008
日本微生物生態学会, Japanese

Pseudomonas sp.T62由来のL‐threo‐3‐hydroxyaspartate dehydrataseの機能解析と分子モデリング
村上知子, 横田篤, 和田大, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2008, 30, 2008
Japanese

Corynebacterium glutamicumのH⁺‐ATPase欠損が呼吸関連酵素活性に与える影響
嘉藤由衣, 和田大, 横田篤, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2008, 19, 2008
Japanese

2C11-3 Molecular cloning of 3-Hydroxyaspartate dehydratase
Wada Masaru, Murakami Tomoko, Maeda Takayuki, Yokota Atsushi, 日本生物工学会大会講演要旨集, 19, 0, 83, 83, 02 Aug. 2007
The Society for Biotechnology, Japan, Japanese

Diversity and mechanisms of alkali tolerance in Lactobacilli (vol 73, pg 3909, 2007)
Yuki Sawatari, Atsushi Yokota, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73, 16, 5385, 5385, Aug. 2007
English, Others

Enhancement of central metabolism by the manipulation of energy metabolism and its application in strain improvement for the production of useful metabolites by fermentation
YOKOTA Atsushi, Bioscience & industry, 65, 6, 276, 281, 01 Jun. 2007
バイオインダストリー協会, Japanese

新規二次胆汁酸生成菌Bacteroides intestinalis AM‐1の二次胆汁酸生成能について
荒田美紀, 吹谷智, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2007, 165, 05 Mar. 2007
Japanese

Corynebacterium glutamicumのピルビン酸キナーゼ欠損株の解析
禅院進, 椎名春樹, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2007, 186, 05 Mar. 2007
Japanese

D‐threo‐3‐ヒドロキシアスパラギン酸デヒドラターゼの探索と精製
前田隆行, 村上知子, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2007, 202, 05 Mar. 2007
Japanese

Corynebacterium glutamicumのH⁺‐ATPase欠損変異株における新奇な中枢代謝活性化機構
横田篤, 李立源, 和田大, 松下一信, 日本農芸化学会大会講演要旨集, 2007, 41, 05 Mar. 2007
Japanese

Corynebacterium glutamicumのH⁺‐ATPase活性低下変異株によるバリン発酵
土方野分, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2007, 186, 05 Mar. 2007
Japanese

大腸菌のH⁺‐ATPase欠損変異株を利用したアラニン発酵~LDH欠損の効果
成田琴美, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2007, 186, 05 Mar. 2007
Japanese

腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構の解明
松原裕樹, 森田直樹, 佐々木泰子, 扇谷悟, 吹谷智, 横田篤, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2007, 2007

ビフィズス菌Bifidobacterium longum内在性プラスミドからの挿入配列の同定と塩基配列解析
吹谷智, 杉山友彦, 加納康正, 横田篤, 日本乳酸菌学会誌, 18, 2, 2007

FISH-フローサイトメトリー法によるラフィノース摂取ヒト糞便における種レベルでのビフィズス菌動態解析
吹谷智, DINOTO Achmad, TATIANA Marques, 渡辺純, 伊藤進, 横田篤, 日本農芸化学会大会講演要旨集, 2007, 2007

2E11-3 Identification and characterization of a putative insertion sequence in a cryptic plasmid from Bifidobacterium longum
FUKIYA Satoru, SUGIYAMA Tomohiko, KANO Yasunobu, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 19, 119, 119, 2007
日本生物工学会, Japanese

Pseudomonas sp.T62由来L‐threo‐3‐hydroxyaspartate dehydratase遺伝子のクローニングと大腸菌での発現
村上知子, 横田篤, 和田大, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2007, 12, 2007
Japanese

ヒト糞便から新規二次胆汁酸生成菌の分離と同定
荒田美紀, 南田公子, 吹谷智, 和田大, 小倉嘉夫, 伊藤喜久治, 内田清久, 横田篤, 日本農芸化学会大会講演要旨集, 2006, 244, 05 Mar. 2006
Japanese

酵母におけるL‐アゼチジン‐2‐カルボン酸アセチルトランスフェラーゼの分布
岡部佳恵, 高木博史, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2006, 250, 05 Mar. 2006
Japanese

Corynebacterium glutamicumのH⁺‐ATPase活性低下変異株によるパリン発酵
土方野分, 青木亮, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2006, 237, 05 Mar. 2006
Japanese

大腸菌のH⁺‐ATPase欠損変異株を利用したアラニン発酵
成田琴美, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2006, 237, 05 Mar. 2006
Japanese

Lactobacillus gasseri JCM1131^Tにおけるコール酸適応現象
松原裕樹, 水谷佳奈子, 森田直樹, 佐々木泰子, 扇谷悟, 吹谷智, 横田篤, 日本農芸化学会大会講演要旨集, 2006, 2006

FISH-フローサイトメトリー法を用いたラフィノース摂取ヒト糞便におけるビフィズス菌の動態解析
ACHMAD Dinoto, Marques Tatiana, 吹谷智, 渡辺純, 伊藤進, 横田篤, 日本農芸化学会大会講演要旨集, 2006, 2006

1E-PM1 Research trends in lactic acid bacteria in post-genome era : comparison between Japan and Europe
YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 18, 75, 75, 2006
日本生物工学会, Japanese

1D10-2 Proteome analysis of a H^+-ATPase-defective mutant of Corynebacterium glutamicum cultured under biotin-sufficient conditions :
LI Liyuan, WADA Masaru, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 18, 60, 60, 2006
日本生物工学会, English

Corynebacterium glutamicum H⁺-ATPase活性不変異株の代謝変化とそのグルタミン酸生産への影響
竹末信親, 青木亮, LI Liyuan, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2006, 2006

2F11-2 Transcriptome analysis of a respiratory chain-manipulated mutant of Escherichia coli K-12
NODA Sakiko, AZUMA Naoki, MATSUCHITA Kazunobu, WADA Masaru, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 17, 0, 25 Sep. 2005
The Society for Biotechnology, Japan, Japanese

呼吸鎖改変による大腸菌の糖代謝活性の向上
東直樹, 野田咲子, 林由香里, 松下一信, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2005, 81, 05 Mar. 2005
Japanese

Corynebacterium glutamicumのH¹‐ATPase変異株によるバリン発酵
青木亮, 土方野分, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2005, 82, 05 Mar. 2005
Japanese

Corynebacterium glutamicumのエネルギー代謝変異株を用いたアミノ酸生産
和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2005, 475, 05 Mar. 2005
Japanese

酵母におけるL‐アゼチジン‐2‐カルボン酸アセチルトランスフェラーゼの分布
岡部佳恵, 高木博史, 横田篤, 和田大, 日本農芸化学会大会講演要旨集, 2005, 221, 05 Mar. 2005
Japanese

発酵性糖による二次胆汁酸生成抑制機構の解明
金子真紀, 結城敏文, 池田満雄, 冨田房男, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2005, 76, 05 Mar. 2005
Japanese

北海道産小麦粉を用いた乳酸菌発酵即席麺の開発
花岡彰宏, 杉山久, 山内宏昭, 横田篤, 澤渡優喜, グリーンテクノ情報, 1, 1, 13, 16, Mar. 2005
Japanese

1G14-2 Development of FISH-flow cytometry coupled method for enumeration of bifindobacteria in human feces
DINOTO Achmad, WATANABE Jun, ITO Susumu, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 17, 192, 192, 2005
日本生物工学会, Japanese

Corynebacterium glutamicumのH⁺-ATPase欠損変異株のグルタミン酸生産時におけるプロテオーム解析
Li Liyuan, 青木亮, 竹末信親, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2005, 2005

酵母におけるL‐アゼチジン‐2‐カルボン酸アセチルトランスフェラーゼの分布
岡部佳恵, 高木博史, 横田篤, 和田大, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2005, 10, 2005
Japanese

Corynebacterium glutamicumのH⁺‐ATPase活性低下変異株によるバリン発酵
土方野分, 青木亮, 和田大, 横田篤, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2005, 11, 2005
Japanese

2K15-2 H^+-ATPase defect in Corynebacterium glutamicum improve L-Valine productivity
AOKI Ryo, WADA Masaru, YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 16, 0, 251, 251, 25 Aug. 2004
The Society for Biotechnology, Japan, Japanese

Bile acid accumulation in enteric lactic acid bacteria and its probiotic relevance
YOKOTA Atsushi, Bioscience & industry, 62, 8, 517, 520, 01 Aug. 2004
バイオインダストリー協会, Japanese

DFA III(Difructose anhydride III)投与における効果
南田公子, 金子真紀, 天野みどり, イヌガ スジャーヤ, 曽根輝雄, 和田大, 横田篤, 原博, 浅野行蔵, 日本農芸化学会大会講演要旨集, 2004, 36, 05 Mar. 2004
Japanese

Levodione reductaseのCorynebacterium glutamicum中での発現と立体選択性
和田大, 片岡道彦, 清水昌, 横田篤, 日本農芸化学会大会講演要旨集, 2004, 182, 05 Mar. 2004
Japanese

Corynebacterium glutamicum ATCC13032へのH⁺‐ATPase活性低下変異の導入
竹末信親, 青木亮, 和田大, 横田篤, 日本農芸化学会大会講演要旨集, 2004, 11, 05 Mar. 2004
Japanese

H^+-ATPase defect in Corynebacterium glutamicum improve L-glutamate productivity
Aoki Ryo, Wada Masaru, Tomita Fusao, Yokota Atsushi, 日本生物工学会大会講演要旨集, 15, 0, 25 Aug. 2003
公益社団法人日本生物工学会, Japanese

Quantitative proteome analysis of Escherichia coli K-12 mutant defective in F_1-ATPase activity
Onoe Kazunori, Wada Masaru, Tomita Fusao, Yokota Atsushi, 日本生物工学会大会講演要旨集, 15, 0, 25 Aug. 2003
公益社団法人日本生物工学会, Japanese

リモニウムペレジー(Limonium perezii)の発根を促進する微生物
イ・ヌガ スジャーヤ, 田中みち子, 曽根輝雄, 和田大, 浅野行蔵, 横田篤, 日本農芸化学会北海道支部・北海道農芸化学協会シンポジウム及び合同学術講演会講演要旨, 2003, 2003

Purification and characterization of the membrane-associated cytochrome c-551 from obligate alkaliphilic Bacillus clarkii K24-1U
Hijikata Shoichi, Matsuno Toshihide, Hara Isao, Hoshino Tamotsu, Matsuyama Hidetoshi, Yokota Atsushi, Yumoto Isao, 日本生物工学会大会講演要旨集, 15, 50, 50, 2003
日本生物工学会, Japanese

エネルギーが欠乏しても糖代謝活性は亢進するメカニズムとは? : 大腸菌から明らかになった呼吸鎖酵素活性の上昇による細胞の「質的変化」
横田 篤, 冨田 房男, 化学と生物, 40, 12, 772, 774, 25 Dec. 2002
学会出版センタ-, Japanese

411 Taxonomic diversity of salt-tolerant yeasts from miso mash
Tanaka Takayuki, Sujaya I Nengah, Tamura Yoshifumi, Yamaki Tazusa, Sone Teruo, Yokota Atsushi, Asano Kouzou, Tomita Fusao, 日本生物工学会大会講演要旨集, 14, 53, 53, 2002
日本生物工学会, Japanese

402 Analysis of the fecal microbiota of human-subjects consuming DFA3
Minamida Kimiko, Nengah Sujaya, Sone Teruo, Asano Kozo, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 14, 51, 51, 2002
日本生物工学会, Japanese

366 Energetics and L-glutamate productivity of a H^+-ATPase defective mutant of Corynebacterium glutamicum
Aoki Ryo, Tanaka Kenji, Tomita Fusao, Yokota Atsushi, 日本生物工学会大会講演要旨集, 14, 43, 43, 2002
日本生物工学会, Japanese

腸内細菌の新機能 : Lactobacillus属乳酸菌による胆汁酸輸送とプロビオティックメカニズム(<特集>現代の乳酸菌研究を斬る)
横田 篤, KURDI Peter, VAN VEEN Hedrik W., 田中 博, MIERAU Igor, KONINGS Wil N., TANNOCK Gerald W., 冨田 房男, 生物工学会誌 : seibutsu-kogaku kaishi, 79, 6, 176, 177, 2001
日本生物工学会, Japanese

803 Molecular cloning and high-level expression in Escherichia coli of fungal α-galactosidase from Absidia corymbifera IFO 8084
Baik Sang Ho, Saito Katsuichi, Sakurai Hiroaki, Sayama Koji, Yokota Atsushi, Asano Kozo, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 180, 180, 2000
日本生物工学会, English

Cloning and analysis of the atp gene coding for H^+-ATPase in Corynebacterium ammoniagenes
Sekine Hironobu, Ishiguro Atsuko, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 80, 80, 2000
日本生物工学会, Japanese

Production of lepidimoide from polysaccharide of okra by filamentous fungus No.12-3.
Yoshimura Makoto, Saito Katsuichi, Tanaka Michiko, Hirose Katsutoshi, Asano Kozo, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 226, 226, 2000
日本生物工学会, Japanese

Culture conditions of intestinal bacteria by anaerobic jar fermentor
Kamijo Manjiro, Kurdi Peter, Asano Kozo, Yokota Atushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 259, 259, 2000
日本生物工学会, Japanese

Screening of microbes for components difficult to degrade in crude oil at low temperature.
Takida Yoshiki, Yokokawa Yasuki, Kurane Ryuichiro, Asano Kozo, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 132, 132, 2000
日本生物工学会, Japanese

542 Purification and Characterization of Nickel Protoporphyrin Degrading Enzyme from Pseudomonas azelaica YA-1
Dedeles Gina Rio, Abe Ayumi, Saito Katsuichi, Suto Manabu, Asano Kozo, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 131, 131, 2000
日本生物工学会, English

Bile acid transport in enteric bacteria and probiotic mechanism
Yokota Atsushi, Kurdi Peter, van Veen Hendrik W., Tanaka Hiroshi, Mierau Igor, Konings Wil N., Tannock Gerald W., Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 373, 373, 2000
日本生物工学会, Japanese

Production of DFA IV from beet sugar by the microbiological methods : Part 1. Production.
Sakurai Hiroaki, Kikuchi Hiroto, Sayama Koji, Asano Kozo, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 54, 54, 2000
日本生物工学会, Japanese

Production of DFA IV from beet sugar by the microbiological methods : Part 2. Purification and properties.
Kikuchi Hiroto, Sakurai Hiroaki, Sayama Koji, Asano Kozo, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 12, 54, 54, 2000
日本生物工学会, Japanese

306 Characterization of Yeast Strains in Alcoholic Fermentation of Brem :
Sujaya I. Nengah, Sone T., Yokota A., Asano K., Tomita F., 日本生物工学会大会講演要旨集, 12, 59, 59, 2000
日本生物工学会, English

A novel screen for the detection of chitin acting antifungal compounds (vol 52, pg 508, 1999)
T Sakurai, N Cheeptham, T Mikawa, A Yokota, F Tomita, JOURNAL OF ANTIBIOTICS, 52, 10, C4, C4, Oct. 1999
English, Others

Membrane-bound ATPase Mutation in Lactococcus lactis and its potential uses as a starter strain
TOMITA Fusao, YOKOTA Atsushi, AMACHI Seigo, 日本農芸化学会誌, 73, 519, 519, 05 Mar. 1999
English

1340 Microbial Degradation of Nickel Protoporphyrin Disodium
Dedeles Gina Rio, Abe Ayumi, Sumita Yoko, Saito Katsuichi, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 11, 314, 314, 1999
日本生物工学会, English

The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation (eds. M. C. Flickinger and S. W. Drew)　　　　　　　　　　　　　　　
John Wiley & Sons, 1999

乳酸菌のATPase欠損変異株の解析 : 酸性条件におけるATPase遺伝子の発現調節
天知 誠吾, 石川 浩平, 豊田 修次, 香川 靖雄, 横田 篤, 冨田 房男, 日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria, 9, 1, 19, 19, 30 Sep. 1998
Japanese

乳酸菌 Lactococcus lactis subsp. lactisのH^+-ATPase遺伝子のクローニング
石川 浩平, 天知 誠吾, 櫻井 博章, 豊田 修次, 横田 篤, 冨田 房男, 日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria, 9, 1, 20, 20, 30 Sep. 1998
Japanese

Mutation in energy metabolism and changes in central metabolism
YOKOTA Atsushi, SEKINE Hironobu, TOMITA Fusao, Bioscience & industry, 56, 3, 29, 31, 01 Mar. 1998
バイオインダストリ-協会, Japanese

3.乳酸菌のATPase欠損変異株の解析 : 酸性条件におけるATPase遺伝子の発現調節(乳酸菌の総合的産業利用に関する研究 : 平成9年度乳酸菌工学研究部会報告)
天知 誠吾, 石川 浩平, 横田 篤, 冨田 房男, 生物工学会誌 : seibutsu-kogaku kaishi, 76, 11, 454, 455, 1998
日本生物工学会, Japanese

Molecular cloning of a DFAIIIase gene from Arthrobacter sp. H65-7
Sumita Yoko, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 10, 275, 275, 1998
日本生物工学会, Japanese

Strategy for diversification of industrial application of lactic acid microorganism
A Ishizaki, K Sonomoto, S Okada, S Amachi, K Ishikawa, A Yokota, F Tomita, K Doi, S Eguchi, N Akiyoshi, F Inagaki, Y Fujio, O Tanaka, S Ohmomo, S Ogata, O Kobayashi, K Hiyama, S Etoh, T Kato, S Shioya, H Shimizu, K Uchiyama, M Morita, K Shimizu, T Suzuki, R Ohashi, T Yamamoto, PM Yin, Y Park, M Okabe, H Ohara, SEIBUTSU-KOGAKU KAISHI, 76, 11, 451, 468, 1998
Japanese, Book review

乳酸菌の膜結合型ATPase欠損変異株に関する研究
天知 誠吾, 横田 篤, 豊田 修次, 冨田 房男, 日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria, 8, 1, 30, 30, 30 Sep. 1997
Japanese

Lactobacillus helveticus に対するlactoperoxidase の影響
河村 泰代, 平野 まゆみ, 天知 誠吾, 横田 篤, 冨田 房男, 日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria, 8, 1, 32, 32, 30 Sep. 1997
Japanese

乳酸菌の総合的産業利用に関する研究--平成8年度乳酸菌工学研究部会報告
天知 誠吾, 横田 篤, 富田 房男, 生物工学会誌, 75, 5, 359, 384, Sep. 1997
日本生物工学会, Japanese

A new perspective on inulin as a carbohydrate resourse
SAKURAI Hiroaki, YOKOTA Atsushi, TOMITA Fusao, Bioscience & industry, 55, 1, 25, 29, 01 Jan. 1997
バイオインダストリ-協会, Japanese

1.乳酸菌の膜結合型ATPase欠損変異株に関する研究(乳酸菌の総合的産業利用に関する研究 : 平成8年度乳酸菌工学研究部会報告
天知 誠吾, 横田 篤, 冨田 房男, 生物工学会誌 : seibutsu-kogaku kaishi, 75, 5, 360, 361, 1997
日本生物工学会, Japanese

Acid tolorance and proton ATPase of lactic acid bacteria.
Amachi Seigo, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 9, 274, 274, 1997
日本生物工学会, Japanese

375 Studies on Antifungal Antibiotics from Ellisiodothis inquinans L1588-A8 :
Cheeptham Naowarat, Higashiyama Takako, Mikawa Takashi, Phay Nyunt, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 9, 123, 123, 1997
日本生物工学会, English

乳酸菌の膜結合型ATPase欠損変異株に関する研究
天知 誠吾, 横田 篤, 冨田 房男, 乳酸菌研究集談会誌, 7, 1, 12, 12, 30 Nov. 1996
Japanese

Arthrobacter sp. H65-7株のInulin fructotransferase (DFA III生産型)遺伝子のクローニング : 酵素
櫻井 博章, 横田 篤, 冨田 房男, 日本農藝化學會誌, 70, 297, 297, 05 Mar. 1996
社団法人日本農芸化学会, Japanese

Arthrobacter nicotinovorans GS-9株のlevan fructotransferaseの精製と性質 : 酵素
斎藤 勝一, 横田 篤, 冨田 房男, 日本農藝化學會誌, 70, 297, 297, 05 Mar. 1996
社団法人日本農芸化学会, Japanese

Strategy for Diversification of Industrial Application of Lactic Acid Microorganism. : Reports of the Research Groups for Fermentation Engineering on Lactic Acid Microorganism in 1995
冨田 房勇, 天知 誠吾, 横田 篤, 生物工学会誌 : seibutsu-kogaku kaishi, 74, 4, 291, 294, 1996
日本生物工学会, Japanese

Purification and properties of DFA III hydrolysis enzyme from Arthrobacter sp. H65-7.
Sakurai Hiroaki, Yokota Atsushi, Matsui Hirokazu, Sumita Yoko, Mori Yumiko, Tomita Fusao, 日本生物工学会大会講演要旨集, 8, 183, 183, 1996
日本生物工学会, Japanese

微好気条件下における含窒素化合物の微生物分解 : 環境科学
山本 千勢子, 我部 政晴, 小原 寿幸, 横田 篤, 冨田 房男, 日本農藝化學會誌, 69, 371, 371, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

大腸菌K-12株のF_1-ATPアーゼ欠損変異株によるピルビン酸発酵 : エネルギー代謝変異株による発酵生産の効率化 : メタボリックエンジニアリング : 有用微生物創製の新展開 : シンポジウム(S-11)
横田 篤, 冨田 房男, 日本農藝化學會誌, 69, 439, 439, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

Arthrobacter nicotinovorans GS-9株によるレバンからのDFAIVの生産 : 微生物
横田 篤, 斎藤 勝一, 後藤 敬子, 冨田 房男, 日本農藝化學會誌, 69, 306, 306, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

大腸菌K-12株のF_1-ATPase欠損変異株のグルコース代謝促進機構の解析 : 微生物
横田 篤, 逸見 将, 冨田 房男, 日本農藝化學會誌, 69, 75, 75, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

乳酸菌のH^+-ATPase欠損変異株に関する研究 : 微生物
横田 篤, 天知 誠吾, 石井 哲, 冨田 房男, 日本農藝化學會誌, 69, 74, 74, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

Leptosphaeria sp.の生産する抗真菌剤 : 有機化学・天然物化学
東山 耕子, Phay Nyunt, 渡部 賢二, 三川 隆, 横田 篤, 冨田 房男, 日本農藝化學會誌, 69, 1, 1, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

ニッケルプロトポルフィリンの微生物分解 : 環境科学
阿部 歩, 山本 千勢子, 斎藤 紘一, 横田 篤, 冨田 房男, 日本農藝化學會誌, 69, 175, 175, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

Enhancement of fermentative production of pyruvic acid by a mutant defective in energy metabolism
YOKOTA Atsushi, TOMITA Fusao, Bioscience & industry, 53, 5, 24, 26, 01 May 1995
バイオインダストリ-協会, Japanese

オリゴ糖の機能と生産に関する研究の最近の潮流
横田 篤, 富田 房男, 日本醸造協会誌, 89, 8, p626, 633, Aug. 1994
日本醸造協会, Japanese

CLONING AND CHARACTERIZATION OF A TRYPTOPHANASE GENE FROM ENTEROBACTER-AEROGENES SM-18 (VOL 139, PG 3275, 1993)
K KAWASAKI, A YOKOTA, S OITA, C KOBAYASHI, S YOSHIKAWA, SI KAWAMOTO, S TAKAO, F TOMITA, MICROBIOLOGY-UK, 140, 999, 999, Apr. 1994
English, Others

Functionally Analysis of Tryptophanase by Site-directed Mutagenesis.
Kawasaki Kosei, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 6, 238, 238, 1994
日本生物工学会, Japanese

Synthesis of a new oligosaccharide by transglycosylation of a levan-degrading enzyme.
Sakurai Masaaki, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 6, 86, 86, 1994
日本生物工学会, Japanese

Tryptophan production by recombinant Escherichia coli having heterospecific tryptophanase gene.
Kawasaki Kosei, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 5, 135, 135, 1993
日本生物工学会, Japanese

微生物による天然多糖からの有用オリゴ糖の生産
富田 房男, 横田 篤, 化学と生物, 30, 3, p170, 175, Mar. 1992
学会出版センタ-, Japanese

Overproduction of Enterobacter aerogenes tryptophanase in Escherichia coli, and its application to enzymatic production of tryptophan.
Kawasaki Kosei, Yokota Atsushi, Tomita Fusao, 日本生物工学会大会講演要旨集, 4, 21, 21, 1992
日本生物工学会, Japanese

Genetic analysis of tryptophanase gene of Enterobacter aerogenes and its expression under trp promoter.
Kawasaki Kosei, Yoshikawa Syuji, Takaoka Naohisa, Yokota Atsushi, Tomita Fusao, 日本醗酵工学会大会講演要旨集, 3, 104, 104, 1991
日本醗酵工学会, Japanese

Production of difructose anhydride III (DFA III) by Arthrobacter sp. H65-7.
Hirayama Tetsu, Yokota Atsushi, Miura Yumiko, Enomoto Koichi, Tomita Fusao, Takao Shoichi, 日本醗酵工学会大会講演要旨集, 234, 234, 1989
日本醗酵工学会, Japanese

Amino acid fermentation
Atsushi Yokota, Masato Ikeda
Springer, 2017, 9784431565185, English, [Editor]

応用微生物学 第３版
横田篤, 大西康夫, 小川順
文永堂出版, Jul. 2016, 4830041315, 327, [Editor]

Lactic acid bacteria and bifidobacteria : current progress in advanced research
Kenji Sonomoto, Atsushi Yokota
Caister Academic Press, 2011, 9781904455820, English, [Editor]

乳酸菌とビフィズス菌のサイエンス　　　　　　　　　　　　　　　
京都大学出版会, 2010, [Joint editor]

Handbook of Corynebacterium glutamicum (eds. L. Eggeling and M. Bott)　　　　　　　　　　　　　　　
CRC Press, 2005, [Contributor]

「微生物利用の大展開」今中忠行 監修　　　　　　　　　　　　　　　
株式会社エヌ・ティー・エス, 2002

「食による生体機能調節の新展開」栄養・食糧科学セレクション3 <安本教傳，葛西隆則 編集>　　　　　　　　　　　　　　　
日本食品出版, 2002

Food for Health in the Pacific Rim (eds. J. R. Whitaker, N. F. Haard, C. F. Shoemaker and R. P. Singh)　　　　　　　　　　　　　　　
Food & Nutrition Press, Inc., 1999

適応的実験室進化による産業微生物のエネルギー欠乏への潜在的な適応能力の解明
科学研究費助成事業
01 Apr. 2021 - 31 Mar. 2024
前田 智也, 横田 篤
本研究では、発酵産業に重要な大腸菌やコリネ型細菌の発酵生産効率の向上を目的として、中枢代謝が活性化している様々な呼吸鎖酵素欠損株の適応的実験室進化を行うことで、産業微生物の細胞増殖と目的物質生産のバランスを最適化させる方法を明らかにすることを最終目的としている。
大腸菌やコリネ型細菌において、酸化的リン酸化の阻害によるエネルギー欠乏の誘導が糖代謝などの中枢代謝を活性化させる一方、著しい増殖悪化を引き起こすことが先行研究から明らかにされている。申請者は、本研究において、エネルギー欠乏株の多くが酢酸を単一炭素源として生育できない、または著しく生育が阻害されることを見出した。酢酸を単一炭素源とした場合、取り込んだ酢酸をまず活性化してアセチルリン酸へ変換する過程で1分子のATPを消費し、その後基質レベルのリン酸化では1分子のATPしか合成できないため、ATP合成は酸化的リン酸化に依存していると考えられる。そのため、酢酸を単一炭素源とする選択圧をかける実験室進化を行うことで、酸化的リン酸化が阻害されているエネルギー欠乏株において、エネルギー欠乏への潜在的な適応能力が活性化された進化株が出現し得るのではないかと考えた。そこで今年度は、まず大腸菌の様々な呼吸鎖酵素を欠損したエネルギー欠乏株6株を親株として、酢酸最少培地を用いた適応的実験室進化を行った。適応的実験室進化は、コントロールとして呼吸鎖を欠損していない野生株を用い、また同一培養条件における反復数4として合計28系列行った。このような適応的実験室進化により、酢酸最少培地における生育が回復または、増殖速度が向上した進化株を取得することに成功した。
日本学術振興会, 基盤研究(C), 北海道大学, 21K06142

Metabolic analysis of an Escherichia coli mutant defective in respiratory chain enzymes and its application to the production of 1,3-butanediol and gamma-aminobutyric acid.
Grants-in-Aid for Scientific Research
01 Apr. 2019 - 31 Mar. 2022
Atsushi Yokota
The respiratory chain of Escherichia coli includes NADH dehydrogenase (NDH) and terminal oxidase. We previously knocked out the highest proton-motive force-generating capacity components (NADH dehydrogenase-1 and cytochrome bo3 oxidase) using the E. coli wild-type W1485 strain. In this study, we found that the double knockout mutant strain (ΔΔ strain) showed an increase in the glucose consumption rate, respiration rate, acetate production, NADH/NAD+ ratio, and glutamate production. A metabolome analysis and RNA-seq analysis revealed that genes encoding for pentose phosphate pathway, acetate metabolism, and gadAB encoding glutamate decarboxylase were significantly increased. We also demonstrated that the ΔΔ strain can be applied for the production of 1,3-butanediol and γ-aminobutyric acid (GABA).
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 19H02863

食事と腸内細菌叢：西欧食による腸内細菌叢崩壊機構の解明
科学研究費助成事業
01 Apr. 2013 - 31 Mar. 2014
横田 篤
日本学術振興会, 基盤研究(B), 北海道大学, 25292042

Development of an innovative method for reducing colon-cancer-inducing secondary bile acid formation through enhancement of anaerobic respiration of the intestinal bacteria
Grants-in-Aid for Scientific Research
2011 - 2012
YOKOTA Atsushi, ISHIZUKA Satoshi
Some secondary bile acids formed in the large intestine are known as a riskfactor of colon cancer. In this study a novel method that reduces such secondary bile acids formed by thereductive metabolism of gut microbes were investigated. We tested if the enhancement of oxidativemetabolism of the responsible gut microbes by the introduction of anaerobic respiration can reduce thetarget secondary bile acids. Thus, rats were fed sodium fumarate-supplemented diet to enhanceanaerobic respiration of the gut microbes. Although the results suggested a possibility of decreasingrelative abundance of deoxycholic acid, a representative colon-cancer-inducing secondary bile acid, theobserved effects were not practically applicable, because high dose of fumarate was required (5~10% ofthe diet), which resulted in the diarrhea in rats experiments. Therefore, improved method of fumaratefeeding, e.g. encapsulation, might be necessary to achieve this goal.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 23658064

Survival strategy of intestinal lactic acid bacteria in the gut : functional analysis of cell surface structure involved in bile acid adaptation
Grants-in-Aid for Scientific Research
2009 - 2011
YOKOTA Atsushi, FUKIYA Satoru, MORITA Naoki, YOKOTA Shin-ichishi
Free bile acids (FBAs) are considered the most deleterious stress compounds that inhibit the growth of intestinal bacteria by their membrane-damaging effect. Previously, we found adaptation in Lactobacillus gasseri JCM1131T to cholic acid (CA), one of the major FBAs in human intestine. In this adaptation, exponentially growing cells were first exposed to 4 mM CA, a sub-lethal concentration, for 30 min and then to lethal concentration at 15 mM. The adapted cells did not show any appreciable decrease in viability in the presence of 15 mM CA even after 7 h, while non-adapted cells rapidly lost viability by the factors of 10-3~-4. In the adapted cells a significant relief of membrane damage was observed in the presence of CA, suggesting alterations in lipid composition in the cell envelope.
Total lipids extracted from adapted and non-adapted cells were separated into three fractions, neutral lipids, glycolipids and phospholipids. Determination of amount of each fraction revealed no significant changes between adapted and non-adapted cells. Likewise, significant differences were neither found in fatty acids compositions of each fraction. However, significant alterations were detected in glycolipids and phospholipids compositions. In glycolipids, increases in the length of sugar chain were detected during the adaptation. Furthermore, a significant increase in relative amount of cardiolipin (CL) was observed in the phospholipid fraction.
To evaluate physiological importance of CL in CA adaptation, vesicle experiment was conducted. It was found that CL confers resistance to the liposome against CA attack, suggesting a pivotal role of CL in adaptation mechanism.
Mutants having deletion in two putative cardiolipin synthase genes (cls) singly or in combination were then derived, and their abilities in CA adaptation were investigated. Although CL levels of the both single-knockout mutants were lower than that of the wild type JCM1131T, the levels were found to increase after exposure to 4 mM CA, and a comparable CA adaptation to the wild type strain was observed in both the mutants. The double-knockout mutant possessed negligible amount of CL but still showed CA adaptation, suggesting a dispensable role of CL in CA adaptation. However, in this mutant, significant decrease in glycolipid fraction and significant increase in phospholipid fraction were observed as compared to wild-type strain and these shifts were more prominent after CA adaptation. Therefore, CL was found to work as a key determinant for the lipid composition of the bacterial cell membrane.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 21380053

Screening of novel secondary bile acid-producing intestinal bacteria and clarification of the mechanism of formation of colon-cancer promoter
Grants-in-Aid for Scientific Research
2004 - 2006
YOKOTA Atsushi, FUKIYA Satoru, YUMOTO Isao
Isolation of novel intestinal bacteria converting primary bile acids such as cholic acid (CA) or chenodeoxycholic acid (CDCA) into colon cancer-promoting secondary bile acids such as deoxycholic acid (DCA) or lithocholic acid (LCA), respectively, was conducted from fecal samples of humans.
Total 619 strains were isolated from fecal samples in anaerobic chamber. Their activities for the formation of secondary bile acids including DCA and LCA were examined by culturing them in the medium containing CA or CDCA followed by the detection of the reaction products by TLC, HPLC and GC-MS analyses. As the results, eight strains were obtained as the secondary bile-acid producers. Determination of 16SrRNA gene sequence revealed that two of them producing DCA or LCA were Clostridium scindens and C.leptum, both of which are known producers for DCA or LCA. The other six strains were found to produce 7-oxo-lithocholic acid (3α-hydroxy-7-oxo-5β-cholanoic acid, 7-keto-LCA) from CDCA. These strains consisted of already know producers for 7-keto-LCA including Escherichia coli(three strains) and Bacteroides fragilis (two strains) and one previously not described strain, Bacteroides intestinalis. Therefore, this strain was selected as a novel producer and designated B. intestinalis AM-1. This strain produced 7-keto-DCA from CA. The same activities were also detected in the type strain of B. intestinalis JCM13265^T
Conversion reaction of strain AM-1 was compared with those of E. coliHB101 and B. fragilis JCM11019^T as the reference strains. It was revealed that strain AM-1 showed conversion yield of more than 90% with lower growth level than the other two strains, resulting in higher activity of conversion per cell than the others. However, we did not observe significant differences in the activities of 7α-hydroxysteroid dehydrogenase, a responsible enzyme producing 7-keto-LCA, in these three strains.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, 16380054

Proleome and transcriptome analyses of an Escherichia coli mutant defective in oxidative phosphorylation
Grants-in-Aid for Scientific Research
2001 - 2002
YOKOTA Atsushi, TOMITA Fusao
Effects of a defect in oxidative phosphorylation (F_1-ATPase defect) on Escherichia coli cells was investigated by proteome and transcriptome analyses.
(1) Sapmple preparation : E. coli K-12 strain W1485 and its F_1-ATPase defective mutant ware cultured in glucose in limited chemostat and used as samples for the analyses.
(2) Proteome analysis : All the expressed proteins were separated by 2 dimentional gel electrophoresis, and the protein spots that showed difference in their densities between the two strains were identified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry.
(3) Transcriptome analysis : Total RNA was extracted from both strains, and ^<30>P-labeled cDNA wsa prepared. DNA array was probed with the prepared cDNA, and the image analysis of the hybridized DNA array was conducted for the analysis of the difference in the gene expression between the two strains.
[Results] Among enzymes involved in glycolysis, several enzymes (phosphoglycerate kinase, enolase, pyruvate kinase I, pyruvate dehydrogenase) showed increased expression both in the level of enzyme activities and in protein amounts (proteome analysis) in the mutant. However, transcriptome analysis showed only pyruvate dehydrogenase expression was increased in the mutant, and no difference was observed in the transcription of the rest of the glycolytic enzymes. Many TCA cycle enzymes (citrate synthase, succinyl-CoA synthetase, malate dehydrogenase, isocitrate lyase, malate synthase) were found to be down-regulated in tha mutant in the levels of enzyme activities, proteome and transcriptome analyses. However, succinate dehydrogenase and fumarate hydratase were found to be up-regulated only in the level of enzyme activities and in proteome analysis. In respiratory chain, expression of NADH dehydrogenase-2 and cytochrome bd oxidase were found to be up-regulated in transcriptome analysis.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), HOKKAIDO UNIVERSITY, 13660072

Strain Improvement and Analysis of Industrial Microorganisms by the Manipulation of Energy Metabolism
Grants-in-Aid for Scientific Research
1998 - 2000
YOKOTA Atsushi, TOMITA Fusao
Effect of the manipulation of H+-ATPase activity, involved in the energy metabolism, on the characteristics of the industrially important bacteria were investigated. The following results obtained suggest that the manipulation of energy metabolism allow us to improve microbial function differently from ordinary way.
1. Escherichia coli : Central metabolism of the F1-ATPase-defective mutant of E.coli was analyzed using the physiologically defined cells cultured continuously in minimal medium. In the mutant increases in the activities of glycolytic enzymes, NADH dehydrogenase of respiratory chain, decreases in the TCA cycle enzyme activities were observed. Based on the genome information, proteome analysis was conducted. The results revealed the changes in the cell protein composition in the mutant.
2. Coryneform-glutantic acid producer : Fermentation profiles of a mutant of Corynebacterium glutamicum defective in H+-ATPase activity was investigated. In the mutant the rate of glucose consumption per cell was found to be enhanced. Glutamic acid production was hardly observed, while the production of pyruvic acid, alanine, lactic acid was increased. All the genes of the H+-ATPase operon were cloned. These genes were inserted into E.coli-Corynebacterium shuttle vector, and wild type strain of C.glutamicum was transformed with this plasmid. The transformant showed 2.7-fold activity of H+-ATPase as that of the wild type.
3. Lactic acid bacteria : A decrease in the H+-ATPase activity in Lactococcus lactis, a starter strain for cheese making, resulted in the acid sensitivity, thereby demonstrating the importance of this enzyme activity on the pH homeostasis of L.lactis. All the genes of H+-ATPase operon of L.lactis were cloned.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B)., HOKKAIDO UNIVERSITY, 10460033

変異株を用いる抗真菌剤の探索
科学研究費助成事業
1998 - 1999
冨田 房男, 横田 篤
先に得られた物質AM-1及びNCA-D6関連物質の単離精製、を行った。
AM-1関連物質と想定されるAM-2を単離した。
AM-2はキチン合成酵素の一部を欠損した株にのみ生育阻害をもたらし、同時に行った野生株には生育阻害は見られなかった。また、その他の糸状菌Aiternaria sp.、Fusarium oxysporum、細菌Salmonella typhymurium、Bacillus subtillisにも生育阻害は見られなかった。AM-2の機器分析の結果分子量が303、分子式がC_<21>H_<37>Nであることがわかった。AM-2は、野生株の粗精製キチン合成酵素反応を阻害することが確認された。また、^3H-uracil、^3H-thymidine、^3H-leucineを用いた取り込み実験の結果、AM-2が3H-uracilと^3H-leucineの取り組みを阻害しており、AM-2によるRNAおよびタンパク合成が影響を受けていると示唆された。
NCA-D6関連の少なくとも4つの物質が確認された。NCA-D6も、キチン合成酵素の一部欠損株にのみ生育阻害をもたらし、同時に行った野生株には生育阻害は見られなかった。また、その他の糸状菌Aiternaria sp.、Fusarium oxysporum、細菌Salmomella typhymurium、Bacillus subtillisにも生育阻害は見られなかった。分子量646、分子式C_<37>H_<58>O_9であり、その構造を明らかにした。NCA-D6は野生株の粗精製キチン合成酵素反応を阻害し また、^3H-uracil、^3H-thymidine、^3H-leucineを用いた取り込み実験の結果、3H-leucineの取り込みを阻害しており、タンパク合成が影響を受けていると示唆された。既知の物質ではあったものの、キチン合成を阻害するという報告は本研究が初めてである。関連物質について単離精製を進め、機器分析にかける段階である。
日本学術振興会, 萌芽的研究, 北海道大学, 10876015

Production of novel oligosaccharides by microbes and evaluation of their function
Grants-in-Aid for Scientific Research
1995 - 1997
TOMITA Fusao, SAYAMA Koji, HARA Hiroshi, KASAI Takanori, YOKOTA Atsushi
1.Preparation of Raw Material for Oligosaccharides Production : Extraction and purification of inulin from chicory was attempted according to the method of sugar purification from beet, and was successfully achieved. Preparation of levan was also carried out by using levan producing bacteria, and levan was recovered and purified from its culture broth. More simple purification process for preparation of raw material has attempted by such method as addition of oligosaccharide-producing enzyme to crude extracts.
2.Screening and Improvement of Oligosaccharide Producing Microbe : Oligosaccharide producing microbe was screened from plant in addition to soil as a screening source, and xylobiose-producing fungi was newly isolated. Molecular cloning of ift and lft gene, which encode DFAIII and DFAIV producing enzyme respectively, were executed. Expression systems of ift and lft gene was constructed in E.coli system, and higher activities were gained by using these system rather these original strains.
3.Establishment of Oligosaccharide Production Process : Culture conditions for oligosaccharides producing enzymes and reaction conditions were optimized by characterizing microbes and enzymes producing DFAIII,DFAIV,inulotriose and levanbiose. Large scale preparations of purified DFAIII and DFAIV were gained by the process such as fermentation of reaction mixturi by yeast, ion exchange column and crystallization.
4.Evaluation of Physiological Function of Oligosaccharide : Properties of DFAIII with respect to both industrial processing and nutritional function by using microbe, rat and human were examined. In the case of rat, calcium absorption was increased by adding DFAIII to feed, and the regions of absorption were determined in large and small intestines. Physiological fuction of DFAIV has also been evaluation.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), HOKKAIDO UNIVERSITY, 07556087

大腸菌及びコリネ型細菌のエネルギー代謝変異株の解析と発酵生産
科学研究費助成事業
1996 - 1996
横田 篤, 須藤 学, 冨田 房男
大腸菌及びコリネ型細菌のH^+-ATPアーゼの活性低下変異株について研究を進め下に示す結果を得た。
1)これまでに当研究室で得られていた大腸菌のH^+-ATPアーゼ欠損株の炭素の流れを解糖系の酸素及び糖の取込み系について解析した。ピルビン酸キナーゼ-1とホスホグリセリン酸キナーゼの活性が元株の約1.5倍、グルコース取込み系(ホスホトランスフェラーゼ系)が約2.1倍、チトクロームb含有量が約1.4倍(呼吸が約1.7倍)に上昇しており、これらの共同作用で炭素の流れ(フラックス)に変化がでたものと結論した。中でもグルコース取込み系の増大は、ピルビン酸の生産向上と協同してフラックスの変化に最も大きな寄与があるものと考えられる。
2)コリネ型細菌のH^+-ATPアーゼの活性が元株の約30%にまで低下した変異株を得てその解析を試みた。本変異株は生育初期には元株よりも生育が遅いが最終的な生育量はほとんど同じであった。本菌においても大腸菌と同様にピルビン酸の生産が増大し、オキソグルタール酸の生産はなくなり、クエン酸サイクルが動いていないことが示された。その結果グルタミン酸の生産はほとんどなくなり、アラニンの生産が増大した。また本菌でも糖消費が増大していたがこれもグルコース取込み系(ホスホトランスフェラーゼ系)の増大が大きく寄与していることが示された。
日本学術振興会, 基盤研究(C), 北海道大学, 08660083

2. 腸内細菌と胆汁酸の相互作用に関する総合的研究　　　　　　　　　　　　　　　
1996
Competitive research funding

大腸菌のエネルギー代謝変異株の解析-糖代謝の新規制御機構と発酵生産の効率化-
科学研究費助成事業
1995 - 1995
横田 篤, 冨田 房男
大腸菌K-12株由来のリボ酸要求性ピルビン酸生産菌を新株として、形質導入法によりF_1-ATPaseの欠損変異遺伝子atpA401を導入して得られたTBLA-1株では、生育は親株の70%に低下したが、菌体当りのグルコース消費速度及びピルビン酸生産速度が親株の2倍及び2.5倍に増大し、その結果ピルビン酸の生産効率が大幅に向上した。平成7年度はTBLA-1株の性質解明とともに、新たに大腸菌K-12株野生株からも同様なF_1-ATPaseの欠損変異株を導き、この株についての性質も検討し、以下の結果を得た。
1.TBLA-1株における解糖系諸酵素活性の変化:グルコースの取込みにかかわるホスホトランスフェラーゼ系を除く解糖系の全ての酵素活性の測定を行い、ホスホグリセリン酸キナーゼ及びピルビン酸キナーゼIの活性が対数増殖期において親株の最大1.5倍に増大していることを認めた。この二つの酵素は共に基質レベルのリン酸化を触媒するものであり、F_1-ATPaseの欠損変異におけるエネルギー(ATP)欠乏を補うレスポンスとして合目的的と考えられた。その他の酵素については両株間で有意な差が認められなかった。
2.大腸菌K-12株野生株からのF_1-ATPase欠損変異株の取得:大腸菌K-12株野生株W1485に、形質導入法によりF_1-ATPaseの欠損変異遺伝子atpA401を導入しHBA-1株を取得した。
3.培養条件の検討:TBLA-1、HBA-1両株の培養条件を検討し、鉄イオンの添加と、有機窒素源としてポリペプトンの添加が有効であることを認めた。
4.HBA-1の糖代謝:3の条件でTBLA-1、HBA-1両株を培養した結果、HBA-1株の培養では著量のピルビン酸が生成した。ピルビン酸生産株でない野生株由来のF_1-ATPaseの欠損株でもピルビン酸が蓄積することから、F_1-ATPaseの欠損株では解糖系が強く活性化されていることが示唆された。
日本学術振興会, 一般研究(C), 北海道大学, 07806010

大腸菌のピルビン酸発酵をモデルとしたエネルギー代謝変異株による発酵生産の効率化
科学研究費助成事業
1994 - 1994
横田 篤, 富田 房男
大腸菌K-12株由来のリポ酸要求性ピルビン酸生産菌を親株として、形質導入法によりF_1-ATPaseの欠損変異遺伝子atpA401を導入して得られたTBLA-1株では、生育は親株の70%に低下したが、菌体当たりのグルコース消費速度およびピルビン酸生産速度が親株の2倍および2.5倍に増大し、その結果ピルビン酸生産効率が大幅に向上した。平成6年度はTBLA-1株の性質を解明することを目的として検討し、次に示す成果を得た。
1.F_1-ATPaseレベルとピルビン酸生産効率との関連性の検討:TBLA-1株から親株の10%の活性を持つF_1-ATPaseの部分復帰変異株を取得した。この株のピルビン酸生産能は親株と同じであった。従って、ピルビン酸生産の効率化のためには、F_1-ATPaseがさらに低下する必要があることが分かった。
2.TBLA-1株における酸素消費量の変化:ピルビン酸生産培養時のTBLA-1株の酸素消費量は親株の1.7倍に増大していた。
3.電子伝達系のチトクロムb含有量の測定:分光学的な測定の結果、TBLA-1株では含有量が親株の3倍に増大していた。2.の結果と合わせて考えると、TBLA-1株では糖消費の増大の結果過剰に生成されるNADHを再酸化するために、これらの変化が起こったと考察された。
4.TBLA-1株における解糖系諸酵素活性の変化:glucose phosphate isomerase,phosphofructokinase,fructose bisphosphate aldolase,phosphoglycerate mutase,pyruvate kinaseの5種類の酵素活性について測定したところ、pyruvate kinaseの活性がTBLA-1において約50%増大しており、解糖系が活性化されていることが見出された。
日本学術振興会, 一般研究(C), 北海道大学, 06806009

Production of useful oligosaccharides from unused polysaccharides by microbial enzymes
Grants-in-Aid for Scientific Research
1992 - 1994
TOMITA Fusao, SUTO Manabu, YOKOTA Atsushi
Oligo-saccharides attract much attention for their physiological function such as anticarious activity, prevention of constipation and of accumulation of plasma cholesterol, and low calorie sweetener etc. It has also been shown that oligo-saccharides are excellent health-care compounds for plants and microbes. As the importance of oligo-saccharides is recognized, it is apparent that development of their production methods becomes important. Thus, we initiated to study oligo-saccharides production by microbial processes using inexpensive starting materials such as inulin and levan. We isolated many microbes degrading inulin or levan from soil samples, and screened oligo-saccharides producers.
1. Oligo-saccharides production from inulin : Arthrobacter sp.H65-7 producing difructose anhydride III (DFA III) was shown to convert DFA III into inulobiose by an intracellular DFA III-degrading enzyme. Inulobiose production from DFA III is possible with whole cell reaction. Streptomyces rochei E87 was found to produce an extracellular enzyme degrading inulin into inulotriose in an exo-manner. We have purified this enzyme and clarified its enzymatic properties.
2. Oligo-saccharides production from levan : Streptomyces exfoliatus F3-2 and Arthrobacter nicotinovorans SG9 were found to produce levanbiose and DFA IV,respectively. Levanbiose-producing enzyme was purified and its enzymatic properties were investigated.
3. Oligo-saccharides production by transfer reaction of degrading enzyme : Arthrobacter sp.MS9 produces a levanbiose-producing enzyme that transfer fructose residue of inulobiose to reducing-terminal fructose of inulobiose by beta2-6 linkage forming a novel trisaccharide.
Japan Society for the Promotion of Science, Grant-in-Aid for General Scientific Research (B), Hokkaido University, 04454074

1. エネルギー代謝に着目した有用微生物の機能解析と産業利用　　　　　　　　　　　　　　　
1988
Competitive research funding