OlCHR, encoding a chromatin remodeling factor, is a killer causing hybrid sterility between rice species Oryza sativa and O. longistaminata.
Zin Mar Myint, Yohei Koide, Wakana Takanishi, Tomohito Ikegaya, Choi Kwan, Kiwamu Hikichi, Yoshiki Tokuyama, Shuhei Okada, Kazumitsu Onishi, Ryo Ishikawa, Daisuke Fujita, Yoshiyuki Yamagata, Hideo Matsumura, Yuji Kishima, Akira Kanazawa
iScience, 27, 5, 109761, 109761, 17 May 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, The genetic mechanisms of reproductive isolation have been widely investigated within Asian cultivated rice (Oryza sativa); however, relevant genes between diverged species have been in sighted rather less. Herein, a gene showing selfish behavior was discovered in hybrids between the distantly related rice species Oryza longistaminata and O. sativa. The selfish allele S13l in the S13 locus impaired male fertility, discriminately eliminating pollens containing the allele S13s from O. sativa in heterozygotes (S13s/S13l). Genetic analysis revealed that a gene encoding a chromatin-remodeling factor (CHR) is involved in this phenomenon and a variety of O. sativa owns the truncated gene OsCHR745, whereas its homologue OlCHR has a complete structure in O. longistaminata. CRISPR-Cas9-mediated loss of function mutants restored fertility in hybrids. African cultivated rice, which naturally lacks the OlCHR homologue, is compatible with both S13s and S13l carriers. These results suggest that OlCHR is a Killer gene, which leads to reproductive isolation.

Pigmentation of soybean seed coats via a mutation that abolishes production of multiple-phased siRNAs of chalcone synthase genes.
Mashiro Yuhazu, Shun Mikuriya, Ayumi Mori, Maria Stefanie Dwiyanti, Mineo Senda, Akira Kanazawa
Genes & Genetic Systems, 99, 23-00260, 23-00260, 29 Mar. 2024, [Peer-reviewed], [Corresponding author], [Domestic magazines]
English, Scientific journal, Lack of pigmentation in seed coats of soybean is caused by natural RNA silencing of chalcone synthase (CHS) genes. This phenomenon is an evolutionary consequence of structural changes in DNA that resulted in the production of double-stranded RNAs (dsRNAs) that trigger RNA degradation. Here we determined that a mutant with pigmented seed coats derived from a cultivar that lacked the pigmentation had a deletion between DNA regions ICHS1 and a cytochrome P450 gene; the deletion included GmIRCHS, a candidate gene that triggers CHS RNA silencing via production of CHS dsRNAs. We also characterized CHS short interfering RNAs (siRNAs) produced in the wild-type seed coats that had CHS RNA silencing. Phased 21-nt CHS siRNAs were detected in all 21 phases and were widely distributed in exon 2 of CHS7, which indicates commonality in the pattern of RNA degradation in natural CHS RNA silencing between distantly related species. These results with the similarities in the rearrangements found in spontaneous mutants suggest that the structural organization that generates dsRNAs that trigger phased siRNA production is vulnerable to further structural changes, which eventually abolish the induction of RNA silencing.

Functional redundancy of R2R3-MYB transcription factors involved in anthocyanin biosynthesis is manifested in anther pigmentation in petunia
Mashiro Yuhazu, Ryoko Hara, Mei Kimura, Akira Kanazawa
Plant Biotechnology, 41, 1, 9, 18, Japanese Society for Plant Cell and Molecular Biology, 25 Mar. 2024, [Peer-reviewed], [Corresponding author]
Scientific journal

Frequency of cytosine methylation in the adjacent regions of soybean retrotransposon SORE-1 depends on chromosomal location
Kenta Nakashima, Mashiro Yuhazu, Shun Mikuriya, Megumi Kasai, Jun Abe, Akito Taneda, Akira Kanazawa
Genome, 67, 1, 1, 12, Canadian Science Publishing, 01 Jan. 2024, [Peer-reviewed], [Corresponding author]
Scientific journal, Mobilization of transposable elements (TEs) is suppressed by epigenetic mechanisms involving cytosine methylation. However, few studies have focused on clarifying relationships between epigenetic influences of TEs on the adjacent DNA regions and time after insertion of TEs into the genome and/or their chromosomal location. Here we addressed these issues using soybean retrotransposon SORE-1. We analyzed SORE-1, inserted in exon 1 of the GmphyA2 gene, one of the newest insertions in this family so far identified. Cytosine methylation was detected in this element but was barely present in the adjacent regions. These results were correlated, respectively, with the presence and absence of the production of short interfering RNAs. Cytosine methylation profiles of 74 SORE-1 elements in the Williams 82 reference genome indicated that methylation frequency in the adjacent regions of SORE-1 was profoundly higher in pericentromeric regions than in euchromatic chromosome arms and was only weakly correlated with the length of time after insertion into the genome. Notably, the higher level of methylation in the 5′ adjacent regions of SORE-1 coincided with the presence of repetitive elements in pericentromeric regions. Together, these results suggest that epigenetic influence of SORE-1 on the adjacent regions is influenced by its location on the chromosome.

Characterization of chlorophyll-deficient soybean [Glycine max (L.) Merr.] mutants obtained by ion-beam irradiation reveals concomitant reduction in isoflavone levels
Yui Shiroshita, Mashiro Yuhazu, Yoshihiro Hase, Tetsuya Yamada, Jun Abe, Akira Kanazawa
GENETIC RESOURCES AND CROP EVOLUTION, 68, 3, 1213, 1223, Mar. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Disturbance of floral colour pattern by activation of an endogenous pararetrovirus, petunia vein clearing virus, in aged petunia plants.
Kazunori Kuriyama, Midori Tabara, Hiromitsu Moriyama, Akira Kanazawa, Hisashi Koiwa, Hideki Takahashi, Toshiyuki Fukuhara
The Plant journal : for cell and molecular biology, 103, 2, 497, 511, Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, White areas of star-type bicolour petals of petunia (Petunia hybrida) are caused by post-transcriptional gene silencing (PTGS) of the key enzyme of anthocyanin biosynthesis. We observed blotched flowers and a vein-clearing symptom in aged petunia plants. To determine the cause of blotched flowers, we focused on an endogenous pararetrovirus, petunia vein clearing virus (PVCV), because this virus may have a suppressor of PTGS (VSR). Transcripts and episomal DNAs derived from proviral PVCVs accumulated in aged plants, indicating that PVCV was activated as the host plant aged. Furthermore, DNA methylation of CG and CHG sites in the promoter region of proviral PVCV decreased in aged plants, suggesting that poor maintenance of DNA methylation activates PVCV. In parallel, de novo DNA methylation of CHH sites in its promoter region was also detected. Therefore, both activation and inactivation of PVCV occurred in aged plants. The accumulation of PVCV transcripts and episomal DNAs in blotched regions and the detection of VSR activity support a mechanism in which suppression of PTGS by PVCV causes blotched flowers.

A Soybean Deletion Mutant That Moderates the Repression of Flowering by Cool Temperatures.
Jingyu Zhang, Meilan Xu, Maria Stefanie Dwiyanti, Satoshi Watanabe, Tetsuya Yamada, Yoshihiro Hase, Akira Kanazawa, Takashi Sayama, Masao Ishimoto, Baohui Liu, Jun Abe
Frontiers in plant science, 11, 429, 429, 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Ambient growing temperature and photoperiod are major environmental stimuli that summer annual crops use to adjust their reproductive phenology so as to maximize yield. Variation in flowering time among soybean (Glycine max) cultivars results mainly from allelic diversity at loci that control photoperiod sensitivity and FLOWERING LOCUS T (FT) orthologs. However, variation in the thermal regulation of flowering and its underlying mechanisms are poorly understood. In this study, we identified a novel mutant (ef1) that confers altered thermal regulation of flowering in response to cool ambient temperatures. Mapping analysis with simple sequence repeat (SSR) markers located the mutation in the upper part of chromosome 19, where no QTL for flowering has been previously reported. Fine-mapping and re-sequencing revealed that the mutation was caused by deletion of a 214 kbp genomic region that contains 11 annotated genes, including CONSTANS-LIKE 2b (COL2b), a soybean ortholog of Arabidopsis CONSTANS. Comparison of flowering times under different photo-thermal conditions revealed that early flowering in the mutant lines was most distinct under cool ambient temperatures. The expression of two FT orthologs, FT2a and FT5a, was dramatically downregulated by cool temperature, but the magnitude of the downregulation was lower in the mutant lines. Cool temperatures upregulated COL2b expression or delayed peak expression, particularly at the fourth trifoliate-leaf stage. Intriguingly, they also upregulated E1, a soybean-specific repressor of FT orthologs. Our results suggest that the ef1 mutation is involved in thermal regulation of flowering in response to cool ambient temperature, and the lack of COL2b in the mutant likely alleviates the repression of flowering by cool temperature. The ef1 mutant can be used as a novel gene resource in breeding soybean cultivars adapted to cool climate and in research to improve our understanding of thermal regulation of flowering in soybean.

Loss of Function of the E1-Like-b Gene Associates With Early Flowering Under Long-Day Conditions in Soybean
Jianghui Zhu, Ryoma Takeshima, Kohei Harigai, Meilan Xu, Fanjiang Kong, Baohui Liu, Akira Kanazawa, Tetsuya Yamada, Jun Abe
Frontiers in Plant Science, 9, 1867, Frontiers Media {SA}, Jan. 2019, [Peer-reviewed]

Transcription of soybean retrotransposon SORE-1 is temporally upregulated in developing ovules.
Kenta Nakashima, Mayumi Tsuchiya, Sae Fukushima, Jun Abe, Akira Kanazawa
Planta, 248, 5, 1331, 1337, Nov. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, MAIN CONCLUSION: Transcription of soybean retrotransposon SORE-1 was temporally upregulated during ovule development. This transcriptional pattern was under intrinsic control conferred by the long terminal repeat of SORE-1. Transcriptionally active retrotransposons are capable of inducing random disruption of genes, providing a powerful tool for mutagenesis. Activation of retrotransposons in reproductive cells, in particular, can lead to heritable changes. Here, we examined developmental control of transcription of soybean retrotransposon SORE-1. Transgenic Arabidopsis plants that contain β-glucuronidase (GUS) reporter gene fused with the SORE-1 long terminal repeat (LTR) had GUS staining in the ovule. Quantitative analysis of transcripts in plants with this DNA construct and those with the full-length SORE-1 element indicated a temporal upregulation of SORE-1 transcription during ovule development. A comparable phenomenon was also observed in soybean plants that had a recent insertion of this element in the GmphyA2 gene. These results provide evidence that the temporal upregulation of SORE-1 in the reproductive organ is sufficiently controlled by its LTR and indicate that the intrinsic expression pattern of SORE-1 is consistent with its mutagenic property.

Chromosomal distribution of soybean retrotransposon SORE-1 suggests its recent preferential insertion into euchromatic regions.
Kenta Nakashima, Jun Abe, Akira Kanazawa
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 26, 3, 199, 210, Sep. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Retrotransposons constitute a large portion of plant genomes. The chromosomal distribution of a wide variety of retrotransposons has been analyzed using genome sequencing data in several plants, but the evolutionary profile of transposition has been characterized for a limited number of retrotransposon families. Here, we characterized 96 elements of the SORE-1 family of soybean retrotransposons using genome sequencing data. Insertion time of each SORE-1 element into the genome was estimated on the basis of sequence differences between the 5' and 3' long terminal repeats (LTRs). Combining this estimation with information on the chromosomal location of these elements, we found that the insertion of the existing SORE-1 into gene-rich chromosome arms occurred on average more recently than that into gene-poor pericentromeric regions. In addition, both the number of insertions and the proportion of insertions into chromosome arms profoundly increased after 1 million years ago. Solo LTRs were detected in these regions at a similar frequency, suggesting that elimination of SORE-1 via unequal homologous recombination was unbiased. Taken together, these results suggest the preference of a recent insertion of SORE-1 into chromosome arms comprising euchromatic regions. This notion is contrary to an earlier view deduced from an overall profiling of soybean retrotransposons and suggests that the pattern of chromosomal distribution can be more diverse than previously thought between different families of retrotransposons.

Lineage-specific gene acquisition or loss is involved in interspecific hybrid sterility in rice.
Yohei Koide, Atsushi Ogino, Takanori Yoshikawa, Yuki Kitashima, Nozomi Saito, Yoshitaka Kanaoka, Kazumitsu Onishi, Yoshihiro Yoshitake, Takuji Tsukiyama, Hiroki Saito, Masayoshi Teraishi, Yoshiyuki Yamagata, Aiko Uemura, Hiroki Takagi, Yoriko Hayashi, Tomoko Abe, Yoshimichi Fukuta, Yutaka Okumoto, Akira Kanazawa
Proceedings of the National Academy of Sciences of the United States of America, 115, 9, E1955-E1962, 27 Feb. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Understanding the genetic basis of reproductive barriers between species has been a central issue in evolutionary biology. The S1 locus in rice causes hybrid sterility and is a major reproductive barrier between two rice species, Oryza sativa and Oryza glaberrima The O. glaberrima-derived allele (denoted S1g) on the S1 locus causes preferential abortion of gametes with its allelic alternative (denoted S1s) in S1g/S1s heterozygotes. Here, we used mutagenesis and screening of fertile hybrid plants to isolate a mutant with an allele, S1mut, which does not confer sterility in the S1mut/S1g and S1mut/S1s hybrids. We found that the causal mutation of the S1mut allele was a deletion in the peptidase-coding gene (denoted "SSP") in the S1 locus of O. glaberrima No orthologous genes of SSP were found in the O. sativa genome. Transformation experiments indicated that the introduction of SSP in carriers of the S1s allele did not induce sterility. In S1mut/S1s heterozygotes, the insertion of SSP led to sterility, suggesting that SSP complemented the loss of the functional phenotype of the mutant and that multiple factors are involved in the phenomenon. The polymorphisms caused by the lineage-specific acquisition or loss of the SSP gene were implicated in the generation of hybrid sterility. Our results demonstrated that artificial disruption of a single gene for the reproductive barrier creates a "neutral" allele, which facilitates interspecific hybridization for breeding programs.

Frequent generation of mutants with coincidental changes in multiple traits via ion-beam irradiation in soybean.
Mikuriya S, Kasai M, Nakashima K, Natasia, Hase Y, Yamada T, Abe J, Kanazawa A
Genes & genetic systems, 92, 3, 153, 161, Jan. 2018, [Peer-reviewed], [Last author, Corresponding author]

RNA silencing in the life cycle of soybean: multiple restriction systems and spatiotemporal variation associated with plant architecture
Ayumi Mori, Hiroshi Sato, Megumi Kasai, Tetsuya Yamada, Akira Kanazawa
TRANSGENIC RESEARCH, 26, 3, 349, 362, Jun. 2017, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II
Kaori Kohzuma, Yutaka Sato, Hisashi Ito, Ayako Okuzaki, Mai Watanabe, Hideki Kobayashi, Michiharu Nakano, Hiroshi Yamatani, Yu Masuda, Yumi Nagashima, Hiroyuki Fukuoka, Tetsuya Yamada, Akira Kanazawa, Keisuke Kitamura, Yutaka Tabei, Masahiko Ikeuchi, Wataru Sakamoto, Ayumi Tanaka, Makoto Kusaba
PLANT PHYSIOLOGY, 173, 4, 2138, 2147, Apr. 2017, [Peer-reviewed]
English, Scientific journal

Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROSI ortholog using a virus vector in Nicotiana benthamiana (vol 4, 44, 2013)
Shungo Otagaki, Megumi Kasai, Chikara Masuta, Akira Kanazawa
FRONTIERS IN GENETICS, 7, Feb. 2016, [Peer-reviewed]
English

"Size and positional effects of promoter RNA segments on virus-induced RNA-directed DNA methylation and transcriptional gene silencing"
Shungo Otagaki, Miou Kawai, Chikara Masuta, Akira Kanazawa
EPIGENETICS, 11, 1, 101, 101, Jan. 2016, [Peer-reviewed]
English

A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog.
Zhao C, Takeshima R, Zhu J, Xu M, Sato M, Watanabe S, Kanazawa A, Liu B, Kong F, Yamada T, Abe J
BMC plant biology, 16, 20, Jan. 2016, [Peer-reviewed]

The Soybean-Specific Maturity Gene E1 Family of Floral Repressors Controls Night-Break Responses through Down-Regulation of FLOWERING LOCUS T Orthologs.
Xu M, Yamagishi N, Zhao C, Takeshima R, Kasai M, Watanabe S, Kanazawa A, Yoshikawa N, Liu B, Yamada T, Abe J
Plant physiology, 168, 4, 1735, 1746, Aug. 2015, [Peer-reviewed]

Profiles of embryonic nuclear protein binding to the proximal promoter region of the soybean β-conglycinin α subunit gene　　　　　　　　　　　　　　　
Yoshino, M, Tsutsumi, K, Kanazawa, A
Plant Biology, 17, 147, 152, 2015, [Peer-reviewed], [Last author, Corresponding author]

Transposon-mediated mutagenesis and its application to plant breeding
Kanazawa Akira, Fukai Eigo, Miyao Akio, Saze Hidetoshi, Tsukiyama Takuji
Breeding Research, 17, 2, 77, 87, Japanese Society of Breeding, 2015, [Peer-reviewed], [Lead author, Corresponding author]
Japanese

Epigenetic Control of Gene Expression and Engineering Novel Traits in Plants
KANAZAWA Akira
KAGAKU TO SEIBUTSU, 52, 4, 241, 248, Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2014, [Peer-reviewed], [Lead author, Last author, Corresponding author]
Japanese, 植物においては，核酸の塩基配列特異的に起きるRNA分解，ならびにDNAのメチル化やヒストン修飾の変化を伴うエピジェネティックな変化を誘導することが可能である．本稿では，これらの反応経路が明らかになった経緯を概説するとともに，後者の例として，ウイルスベクターを用いてRNA-directed DNA methylationを誘導し，外来遺伝子をもたずに特定の内在性遺伝子の発現が抑制された植物を作出した研究を紹介する．また，遺伝子特異的，あるいは非特異的にエピジェネティックな変化を誘導する方法，ならびにCRISPR/Cas系などによるゲノム編集を含む，新規な植物育種技術の有効性と特徴を論じる．

Genetic variation in four maturity genes affects photoperiod insensitivity and PHYA-regulated post-flowering responses of soybean.
Xu M, Xu Z, Liu B, Kong F, Tsubokura Y, Watanabe S, Xia Z, Harada K, Kanazawa A, Yamada T, Abe J
BMC plant biology, 13, June, 91, 25 Jun. 2013, [Peer-reviewed]
English

Deep sequencing uncovers commonality in small RNA profiles between transgene-induced and naturally occurring RNA silencing of chalcone synthase-A gene in petunia
Megumi Kasai, Hideo Matsumura, Kentaro Yoshida, Ryohei Terauchi, Akito Taneda, Akira Kanazawa
BMC genomics, 14, 63, 63, 30 Jan. 2013, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, BACKGROUND: Introduction of a transgene that transcribes RNA homologous to an endogenous gene in the plant genome can induce silencing of both genes, a phenomenon termed cosuppression. Cosuppression was first discovered in transgenic petunia plants transformed with the CHS-A gene encoding chalcone synthase, in which nonpigmented sectors in flowers or completely white flowers are produced. Some of the flower-color patterns observed in transgenic petunias having CHS-A cosuppression resemble those in existing nontransgenic varieties. Although the mechanism by which white sectors are generated in nontransgenic petunia is known to be due to RNA silencing of the CHS-A gene as in cosuppression, whether the same trigger(s) and/or pattern of RNA degradation are involved in these phenomena has not been known. Here, we addressed this question using deep-sequencing and bioinformatic analyses of small RNAs. RESULTS: We analyzed short interfering RNAs (siRNAs) produced in nonpigmented sectors of petal tissues in transgenic petunia plants that have CHS-A cosuppression and a nontransgenic petunia variety Red Star, that has naturally occurring CHS-A RNA silencing. In both silencing systems, 21-nt and 22-nt siRNAs were the most and the second-most abundant size classes, respectively. CHS-A siRNA production was confined to exon 2, indicating that RNA degradation through the RNA silencing pathway occurred in this exon. Common siRNAs were detected in cosuppression and naturally occurring RNA silencing, and their ranks based on the number of siRNAs in these plants were correlated with each other. Noticeably, highly abundant siRNAs were common in these systems. Phased siRNAs were detected in multiple phases at multiple sites, and some of the ends of the regions that produced phased siRNAs were conserved. CONCLUSIONS: The features of siRNA production found to be common to cosuppression and naturally occurring silencing of the CHS-A gene indicate mechanistic similarities between these silencing systems especially in the biosynthetic processes of siRNAs including cleavage of CHS-A transcripts and subsequent production of secondary siRNAs in exon 2. The data also suggest that these events occurred at multiple sites, which can be a feature of these silencing phenomena.

Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants
Kasai Megumi, Kanazawa Akira
Plant Biotechnology, 30, 3, 233, 241, Japanese Society for Plant Biotechnology, 2013, [Peer-reviewed], [Last author, Corresponding author]
English, Gene silencing through transcriptional repression can be induced by double-stranded RNA (dsRNA) that targets a gene promoter. This phenomenon, termed RNA-mediated transcriptional gene silencing (TGS), was first discovered in plants using a transgene that transcribes an inverted repeat of promoter sequence. However, endogenous genes differ from transgenes in the feasibility of TGS induction, by being more resistant to silencing. Heritable, transgenerational silencing of an endogenous gene has been induced by targeting dsRNA to the promoter in petunia and tomato plants, using a vector based on Cucumber mosaic virus. Efficient TGS depends on the function of a viral protein, which can facilitate epigenetic modifications through the transport of short interfering RNA to the nucleus. The efficiency of the TGS also depends on the length and nucleotide composition of the promoter RNA segments. Such epigenetic changes induced by the viral vector results in a novel class of modified plant, a plant that does not carry a transgene but has altered traits. Thus, TGS to modify the epigenetic state of a plant is now a feasible tool to engineer novel traits. Here we review epigenetic changes induced in a particular gene through RNA-directed DNA methylation and those induced randomly on the genome in terms of their use for plant biotechnology.

An Active CACTA-Family Transposable Element is Responsible for Flower Variegation in Wild Soybean Glycine soja
Ryoji Takahashi, Yasumasa Morita, Masayoshi Nakayama, Akira Kanazawa, Jun Abe
PLANT GENOME, 5, 2, 62, 70, Jul. 2012, [Peer-reviewed]
English, Scientific journal

Complex genetic nature of sex-independent transmission ratio distortion in Asian rice species: the involvement of unlinked modifiers and sex-specific mechanisms
Y. Koide, Y. Shinya, M. Ikenaga, N. Sawamura, K. Matsubara, K. Onishi, A. Kanazawa, Y. Sano
HEREDITY, 108, 3, 242, 247, Mar. 2012, [Peer-reviewed]
English, Scientific journal

In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation by genistein
Sachiko Arase, Megumi Kasai, Akira Kanazawa
PLANT METHODS, 8, 10, Mar. 2012, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Coincident sequence-specific RNA degradation of linked transgenes in the plant genome
Megumi Kasai, Maiko Koseki, Kazunori Goto, Chikara Masuta, Shiho Ishii, Roger P. Hellens, Akito Taneda, Akira Kanazawa
PLANT MOLECULAR BIOLOGY, 78, 3, 259, 273, Feb. 2012, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Establishment of an efficient in vitro culture and particle bombardment-mediated transformation systems in Miscanthus sinensis Anderss., a potential bioenergy crop
Xun Wang, Tetsuya Yamada, Fan-Jiang Kong, Yuki Abe, Yoichiro Hoshino, Hiroko Sato, Tadashi Takamizo, Akira Kanazawa, Toshihiko Yamada
GLOBAL CHANGE BIOLOGY BIOENERGY, 3, 4, 322, 332, Aug. 2011, [Peer-reviewed]
English, Scientific journal

Size and positional effects of promoter RNA segments on virus-induced RNA-directed DNA methylation and transcriptional gene silencing
Shungo Otagaki, Miou Kawai, Chikara Masuta, Akira Kanazawa
EPIGENETICS, 6, 6, 681, 691, Jun. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Optimization of ion-beam irradiation for mutagenesis in soybean: effects on plant growth and production of visibly altered mutants
Sachiko Arase, Yoshihiro Hase, Jun Abe, Megumi Kasai, Tetsuya Yamada, Keisuke Kitamura, Issay Narumi, Atsushi Tanaka, Akira Kanazawa
PLANT BIOTECHNOLOGY, 28, 3, 323, 329, 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants
Akira Kanazawa, Jun-ichi Inaba, Hanako Shimura, Shungo Otagaki, Sayuri Tsukahara, Akihiko Matsuzawa, Bo Min Kim, Kazunori Goto, Chikara Masuta
PLANT JOURNAL, 65, 1, 156, 168, Jan. 2011, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

RNA-mediated epigenetic modifications of an endogenous gene targeted by a viral vector: A potent gene silencing system to produce a plant that does not carry a transgene but has altered traits
Akira Kanazawa, Jun-ichi Inaba, Megumi Kasai, Hanako Shimura, Chikara Masuta
Plant Signaling and Behavior, 6, 8, 1090, 1093, Landes Bioscience, 2011, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

RNA silencing as a tool to uncover gene function and engineer novel traits in soybean
Megumi Kasai, Akira Kanazawa
BREEDING SCIENCE, 61, 5, 468, 479, Jan. 2011, [Peer-reviewed], [Last author, Corresponding author]
English

Two Coordinately Regulated Homologs of FLOWERING LOCUS T Are Involved in the Control of Photoperiodic Flowering in Soybean
Fanjiang Kong, Baohui Liu, Zhengjun Xia, Shusei Sato, Bo Min Kim, Satoshi Watanabe, Tetsuya Yamada, Satoshi Tabata, Akira Kanazawa, Kyuya Harada, Jun Abe
PLANT PHYSIOLOGY, 154, 3, 1220, 1231, Nov. 2010, [Peer-reviewed]
English, Scientific journal

The Soybean Stem Growth Habit Gene Dt1 Is an Ortholog of Arabidopsis TERMINAL FLOWER1
Baohui Liu, Satoshi Watanabe, Tomoo Uchiyama, Fanjiang Kong, Akira Kanazawa, Zhengjun Xia, Atsushi Nagamatsu, Maiko Arai, Tetsuya Yamada, Keisuke Kitamura, Chikara Masuta, Kyuya Harada, Jun Abe
PLANT PHYSIOLOGY, 153, 1, 198, 210, May 2010, [Peer-reviewed]
English, Scientific journal

Fertility restoration by Ifr1 in rice with BT-type cytoplasmic male sterility is associated with a reduced level, but not processing, of atp6-orf79 co-transcribed RNA
Haruka Ohta, Atsushi Ogino, Megumi Kasai, Yoshio Sano, Akira Kanazawa
PLANT CELL REPORTS, 29, 4, 359, 369, Apr. 2010, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Adaptive evolution as a consequence of gene duplication and subsequent insertion of a novel Ty1/copia-like retrotransposon in soybean
Kanazawa Akira, Liu Baohui, Kong Fanjiang, Arase Sachiko, Abe Jun
GENES & GENETIC SYSTEMS, 84, 6, 465, Dec. 2009, [Peer-reviewed]

Adaptive Evolution Involving Gene Duplication and Insertion of a Novel Ty1/copia-Like Retrotransposon in Soybean
Akira Kanazawa, Baohui Liu, Fanjiang Kong, Sachiko Arase, Jun Abe
JOURNAL OF MOLECULAR EVOLUTION, 69, 2, 164, 175, Aug. 2009, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Down-regulation of flavonoid 3′-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence
Atsushi Nagamatsu, Chikara Masuta, Hideyuki Matsuura, Keisuke Kitamura, Jun Abe, Akira Kanazawa
Journal of Plant Physiology, 166, 1, 32, 39, Elsevier BV, Jan. 2009, [Peer-reviewed], [Last author, Corresponding author]

Spatial and temporal control of transcription of the soybean beta-conglycinin alpha subunit gene is conferred by its proximal promoter region and accounts for the unequal distribution of the protein during embryogenesis
Yusuke Imoto, Tetsuya Yamada, Keisuke Kitamura, Akira Kanazawa
GENES & GENETIC SYSTEMS, 83, 6, 469, 476, Dec. 2008, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

RNA silencing of a gene involved in flavonoid synthesis reveals the presence of a threshold mRNA level associated with pigmentation in plant tissues
Nagamatsu Atsushi, Masuta Chikara, Matsuura Hideyuki, Kitamura Keisuke, Abe Jun, Kanazawa Akira
GENES & GENETIC SYSTEMS, 83, 6, 515, Dec. 2008, [Peer-reviewed]

Genetic Redundancy in Soybean Photoresponses Associated With Duplication of the Phytochrome A Gene
Baohui Liu, Akira Kanazawa, Hisakazu Matsumura, Ryoji Takahashi, Kyuya Harada, Jun Abe
GENETICS, 180, 2, 995, 1007, Oct. 2008, [Peer-reviewed]
English, Scientific journal

The evolution of sex-independent transmission ratio distortion involving multiple allelic interactions at a single locus in rice
Yohei Koide, Mitsunobu Ikenaga, Noriko Sawamura, Daisuke Nishimoto, Kazuki Matsubara, Kazumitsu Onishi, Akira Kanazawa, Yoshio Sano
GENETICS, 180, 1, 409, 420, Sep. 2008, [Peer-reviewed]
English, Scientific journal

RNA silencing manifested as visibly altered phenotypes in plants
Akira Kanazawa
PLANT BIOTECHNOLOGY, 25, 5, 423, 435, 2008, [Peer-reviewed], [Lead author, Last author, Corresponding author]
English

Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass
Hiroshi Hisano, Akira Kanazawa, Midori Yoshida, Mervyn O. Humphreys, Masaru Iizuka, Keisuke Kitamura, Toshihiko Yamada
NEW PHYTOLOGIST, 178, 4, 766, 780, 2008, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Sex-independent transmission ratio distortion system responsible for reproductive barriers between Asian and African rice species
Yohei Koide, Kazumitsu Onishi, Daisuke Nishimoto, Akhil Ranjan Baruah, Akira Kanazawa, Yoshio Sano
NEW PHYTOLOGIST, 179, 3, 888, 900, 2008, [Peer-reviewed]
English, Scientific journal

Genetics of speciation in rice
Yohei Koide, Kazumitsu Onishi, Akira Kanazawa, Yoshio Sano
Biotechnology in Agriculture and Forestry, 62, 247, 259, Springer International Publishing, 2008, [Peer-reviewed]
English, In book

Temporal and spatial regulation of the soybean seed storage protein β-conglycinin α subunit gene promoter in transgenic Arabidopsis thaliana
Imoto Y, Kitamura K, Kanazawa A
Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 48, 105, 106, The Crop Science Society of Japan, Dec. 2007
Japanese

Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing
Atsushi Nagamatsu, Chikara Masuta, Mineo Senda, Hideyuki Matsuura, Atsushi Kasai, Sung Hong, Keisuke Kitamura, Jun Abe, Akira Kanazawa
PLANT BIOTECHNOLOGY JOURNAL, 5, 6, 778, 790, Nov. 2007, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Epigenetic inactivation of chalcone synthase-A transgene transcription in petunia leads to a reversion of the post-transcriptional gene silencing phenotype
Akira Kanazawa, Michael O'Dell, Roger P. Hellens
PLANT AND CELL PHYSIOLOGY, 48, 4, 638, 647, Apr. 2007, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

The binding of nuclear factors to the as-1 element in the CaMV 35S promoter is affected by cytosine methylation in vitro　　　　　　　　　　　　　　　
Kanazawa, A, O’Dell, M, Hellens, R. P
Plant Biology, 9, 435, 441, 2007, [Peer-reviewed], [Lead author, Corresponding author]

Relationship between methylation status of a transgene promoter and the stability of transcriptional gene silencing in plants
Otagaki S, Masuta C, Kanazawa A
Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 47, 49, 50, The Crop Science Society of Japan, Dec. 2006
Japanese

Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation
H Shinozuka, H Hisano, S Yoneyama, Y Shimamoto, ES Jones, JW Forster, T Yamada, A Kanazawa
MOLECULAR GENETICS AND GENOMICS, 275, 4, 399, 408, Apr. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

The regulatory function of the upstream sequence of the beta-conglycinin alpha subunit gene in seed-specific transcription is associated with the presence of the RY sequence
Michiko Yoshino, Atsushi Nagamatsu, Ken-ichi Tsutsumi, Akira Kanazawa
GENES & GENETIC SYSTEMS, 81, 2, 135, 141, Apr. 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector
Shungo Otagaki, Makoto Arai, Akiko Takahashi, Kazunori Goto, Jin-Sung Hong, Chikara Masuta, Akira Kanazawa
Plant Biotechnology, 23, 3, 259, 265, Japanese Society for Plant Cell and Molecular Biology, 2006, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Induction and maintenance of transcriptional repression of a transgene by RNA-directed DNA methylation depend on the target sequence in the promoter
Otagaki S, Hong J S, Goto K, Masuta C, Kanazawa A
Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 46, 65, 66, The Crop Science Society of Japan, Dec. 2005
Japanese

The star-type color pattern in Petunia hybrida 'Red star' flowers is induced by sequence-specific degradation of chalcone synthase RNA
M Koseki, K Goto, C Masuta, A Kanazawa
PLANT AND CELL PHYSIOLOGY, 46, 11, 1879, 1883, Nov. 2005, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Molecular analysis and physicochemical properties of electrophoretic variants of wild soybean Glycine soja storage proteins
T Fukuda, N Maruyama, A Kanazawa, J Abe, Y Shimamoto, M Hiemori, H Tsuji, T Tanisaka, S Utsumi
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 53, 9, 3658, 3665, May 2005, [Peer-reviewed]
English, Scientific journal

Induction of transcriptional repression on a transgene promoter using a plant virus vector.
Otagaki S, Hong JS, Goto K, Masuta C, Kanazawa A
Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 45, 109, 110, The Crop Science Society of Japan, Dec. 2004
Japanese

Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing
H Hisano, A Kanazawa, A Kawakami, M Yoshida, Y Shimamoto, T Yamada
PLANT SCIENCE, 167, 4, 861, 868, Oct. 2004, [Peer-reviewed]
English, Scientific journal

Changes in the Structural Properties of Chloroplasts and Mitochondria in Response to Low Temperature in Perennial Ryegrass (Lolium perenne L.)
Tominaga Yoko, Kanazawa Akira, Shimamoto Yoshiya
Journal of Japanese Society of Grassland Science, 50, 1, 31, 39, Japanese Society of Grassland Science, 15 Apr. 2004, [Peer-reviewed], [Corresponding author]
English, Changes in the ultrastructural properties of chloro-plasts and mitochondria in response to low temperature were analyzed by transmission electron microscopy in perennial ryegrass (Lolium perenne L.). Specimens from plants treated at 4℃ for 24 hr revealed increases in the sizes of chloroplasts in mesophyll cells and mitochondria in mesophyll and vascular bundle sheath cells. Longer cold treatments caused smaller amount of changes in the sizes of these organelles. The enlargement of chloroplasts in mesophyll cells was accompanied by a proportional decrease in the size of vacuoles. The lamella...

Marker-assisted analysis for soybean hard seededness with isozyme and simple sequence repeat loci
SI Sakamoto, J Abe, A Kanazawa, Y Shimamoto
BREEDING SCIENCE, 54, 2, 133, 139, 2004, [Peer-reviewed]
English, Scientific journal

High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima
Hisano, H, Kimoto, Y, Hayakawa, H, Takeichi, J, Domae, T, Hashimoto, R, Abe, J, Asano, S, Kanazawa, A, Shimamoto, Y
Plant Cell Reports, 22, 12, 910, 918, Springer Berlin / Heidelberg, 2004, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Communicated by H. UchimiyaAbstract We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and -glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.

Phylogenetic relationships of the chloroplast genomes in the genus Glycine inferred from four intergenic spacer sequences
M Sakai, A Kanazawa, A Fujii, FS Thseng, J Abe, Y Shimamoto
PLANT SYSTEMATICS AND EVOLUTION, 239, 1-2, 29, 54, Jul. 2003, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Photoperiod-insensitive Japanese soybean landraces differ at two maturity loci　　　　　　　　　　　　　　　
Abe, J, Xu, D, Miyano, A, Komatsu, K, Kanazawa, A, Shimamoto, Y
Crop Science, 43, 1300, 1304, 2003, [Peer-reviewed]

A simple and rapid method to detect plant siRNAs using nonradioactive probes　　　　　　　　　　　　　　　
Goto, K, Kanazawa, A, Kusaba, M, Masuta, C
Plant Molecular Biology Reporter, 21, 51, 58, 2003, [Peer-reviewed], [Corresponding author]

Soybean germplasm pools in Asia revealed by nuclear SSRs　　　　　　　　　　　　　　　
Abe, J, Xu, D. H, Suzuki, Y, Kanazawa, A, Shimamoto, Y
Theoretical and Applied Genetics, 106, 445, 453, 2003, [Peer-reviewed]

Structural variation around the gene encoding the alpha subunit of soybean beta-conglycinin and correlation with the expression of the alpha subunit
M Yoshino, A Kanazawa, K Tsutsumi, Nakamura, I, K Takahashi, Y Shimamoto
BREEDING SCIENCE, 52, 4, 285, 292, Dec. 2002, [Peer-reviewed]
English, Scientific journal

Identification of Cold-responsive Genes in Perennial Ryegrass (Lolium perenne L.) by a Modified Differential Display Method
TOMINAGA Yoko, KANAZAWA Akira, SHIMAMOTO Yoshiya
Journal of Japanese Society of Grassland Science, 47, 5, 516, 519, Japanese Society of Grassland Science, 15 Dec. 2001, [Peer-reviewed]
English

Structure and characterization of the gene encoding alpha subunit of soybean beta-conglycinin
M Yoshino, A Kanazawa, K Tsutsumi, Nakamura, I, Y Shimamoto
GENES & GENETIC SYSTEMS, 76, 2, 99, 105, Apr. 2001, [Peer-reviewed]
English, Scientific journal

Identification of sequence variations by PCR-RFLP and its application to the evaluation of cpDNA diversity in wild and cultivated soybeans　　　　　　　　　　　　　　　
Xu, D. H, Abe, J, Kanazawa, A, Gai, J. Y, Shimamoto, Y
Theoretical and Applied Genetics, 102, 683, 688, 2001, [Peer-reviewed]

Mini-scale method for nuclear run-on transcription assay in plants　　　　　　　　　　　　　　　
Kanazawa, A, O’Dell, M, Hellens, R. P, Hitchin, E, Metzlaff, M
Plant Molecular Biology Reporter, 18, 377, 383, 2000, [Peer-reviewed], [Lead author, Corresponding author]

Sequence variation of non-coding regions of chloroplast DNA of soybean and related wild species and its implications for the evolution of different chloroplast haplotypes　　　　　　　　　　　　　　　
Xu, D, Abe, J, Sakai, M, Kanazawa, A, Shimamoto, Y
Theoretical and Applied Genetics, 101, 724, 732, 2000, [Peer-reviewed]

Inter- and intra-specific distribution of Stowaway transposable elements in AA-genome species of wild rice　　　　　　　　　　　　　　　
Kanazawa, A, Akimoto, M, Morishima, H, Shimamoto, Y
Theoretical and Applied Genetics, 101, 327, 335, 2000, [Peer-reviewed], [Lead author]

Soybean recombination sites are present as dispersed segments in Arabidopsis and liverwort mitochondrial DNA　　　　　　　　　　　　　　　
Kanazawa, A, Shimamoto, Y
Plant Molecular Biology Reporter, 17, 19, 29, 1999, [Peer-reviewed], [Lead author, Corresponding author]

Phylogenetic relationships of the mitochondrial genomes in the genus Glycine subgenus Soja
A Kanazawa, A Tozuka, S Akimoto, J Abe, Y Shimamoto
GENES & GENETIC SYSTEMS, 73, 4, 255, 261, Aug. 1998, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Rapid Decrease in DNA Content Induced by Gamma Irradiation in Cultured Cells of Tobacco
KANAZAWA Akira, HATANAKA Mitsuhiro, TSUTSUMI Nobuhiro, EGUCHI Hoshio, HIRAI Atsushi
Radioisotopes, 47, 5, 399, 404, 日本アイソト-プ協会, May 1998, [Peer-reviewed], [Lead author]
English

Sequence variation of chloroplast DNA that involves EcoRI and ClaI restriction site polymorphisms in soybean
A Kanazawa, A Tozuka, Y Shimamoto
GENES & GENETIC SYSTEMS, 73, 2, 111, 119, Apr. 1998, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA
A Kanazawa, S Watanabe, T Nakamoto, N Tsutsumi, A Hirai
GENES & GENETIC SYSTEMS, 73, 1, 39, 44, Feb. 1998, [Peer-reviewed], [Lead author]
English, Scientific journal

Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA
A Tozuka, H Fukushi, T Hirata, M Ohara, A Kanazawa, T Mikami, J Abe, Y Shimamoto
THEORETICAL AND APPLIED GENETICS, 96, 2, 170, 176, Feb. 1998, [Peer-reviewed]
English, Scientific journal

Evolutionary changes in the structures of the cox2 and atp6 loci in the mitochondrial genome of soybean involving recombination across small interspersed sequences　　　　　　　　　　　　　　　
Kato, S, Kanazawa, A, Mikami, T, Shimamoto, Y
Current Genetics, 34, 303, 312, 1998, [Peer-reviewed], [Corresponding author]

Multiple changes in tobacco mitochondrial DNA populations during tissue culture　　　　　　　　　　　　　　　
Kanazawa, A
Plant Breeding, 117, 469, 472, 1998, [Peer-reviewed], [Lead author, Last author, Corresponding author]

RFLPs of chloroplast and mitochondrial DNA in wild soybean, Glycine soja, growing in China　　　　　　　　　　　　　　　
Shimamoto, Y, Fukushi, H, Abe, J, Kanazawa, A, Gai, J, Gao, Z, Xu, D
Genetic Resources and Crop Evolution, 45, 433, 439, 1998, [Peer-reviewed]

Differential changes in copy numbers of rice mitochondrial plasmid-like DNAs and main mitochondrial genomic DNAs that depend on temperature　　　　　　　　　　　　　　　
Kanazawa, A, Tsutsumi, N, Hirai, A
33, 437, 444, 1998, [Peer-reviewed], [Lead author]

Small interspersed sequences that serve as recombination sites at the cox2 and atp6 loci in the mitochondrial genome of soybean are widely distributed in higher plants　　　　　　　　　　　　　　　
Kanazawa, A, Tozuka, A, Kato, S, Mikami, T, Abe, J, Shimamoto, Y
Current Genetics, 33, 188, 198, 1998, [Peer-reviewed], [Lead author, Corresponding author]

Changes in the composition of the population of mtDNAs during callus culture and regeneration in tobacco
Kanazawa A.
Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 38, 96, 97, The Crop Science Society of Japan, Dec. 1997
Japanese

Mitochondrial plasmid-like DNAs of the B1 family in the genus Oryza: Sequence heterogeneity and evolution
S Miyata, A Kanazawa, N Tsutsumi, Y Sano, A Hirai
JAPANESE JOURNAL OF GENETICS, 70, 6, 675, 685, Dec. 1995, [Peer-reviewed]
English, Scientific journal

POLYMORPHIC DISTRIBUTION AND MOLECULAR DIVERSIFICATION OF MITOCHONDRIAL PLASMID-LIKE DNAS IN THE GENUS ORYZA
S MIYATA, A KANAZAWA, N TSUTSUMI, Y SANO, A HIRAI
JAPANESE JOURNAL OF GENETICS, 70, 5, 601, 614, Oct. 1995, [Peer-reviewed]
English, Scientific journal

Reversible changes in the composition of the population of mtDNAs during dedifferentiation and regeneration in tobacco　　　　　　　　　　　　　　　
Kanazawa, A, Tsutsumi, N, Hirai, A
Genetics, 138, 865, 870, 1994, [Peer-reviewed], [Lead author, Corresponding author]

イネ・ミトコンドリアのプラスミド様DNA
金沢 章, 平井 篤志
バイオサイエンスとインダストリ-, 51, 5, p404, 406, バイオインダストリ-協会, May 1993, [Peer-reviewed], [Lead author]
Japanese

Restriction fragments homologous to mitochondrial plasmid-like DNAs are located within limited chromosomal regions on the rice nuclear genome　　　　　　　　　　　　　　　
Kanazawa, A, Kishimoto, N, Sakamoto, W, Ohsawa, R, Ukai, Y, Tsutsumi, N, Hirai, A, Saito, A
Theoretical and Applied Genetics, 87, 577, 586, 1993, [Peer-reviewed], [Lead author, Corresponding author]

Extraction of restrictable DNA from plants of the genusNelumbo
Akira Kanazawa, Nobuhiro Tsutsumi
Plant Molecular Biology Reporter, 10, 4, 316, 318, Springer Science and Business Media LLC, Nov. 1992, [Peer-reviewed], [Lead author]

DISTRIBUTION AND QUANTITATIVE VARIATION OF MITOCHONDRIAL PLASMID-LIKE DNAS IN CULTIVATED RICE (ORYZA-SATIVA L)
A KANAZAWA, W SAKAMOTO, M NAKAGAHRA, K KADOWAKI, N TSUTSUMI, S TANO
JAPANESE JOURNAL OF GENETICS, 67, 4, 309, 319, Aug. 1992, [Peer-reviewed], [Lead author]
English, Scientific journal

Linkage analysis of the nuclear homologues of mitochondrial plasmid-like DNAs in rice
KANAZAWA Akira, SAKAMOTO Wataru, KISHIMOTO Naoki, YANO Masahiro, TSUTSUMI Nobuhiro, SAITO Akira, TANO Shigemitsu
The Japanese Journal of Genetics, 66, 5, 597, 607, The Genetics Society of Japan, 1991, [Peer-reviewed], [Lead author]
English, A genetical study on the nucleotide sequences of the nuclear DNAs which share homology with rice mitochondrial plasmid-like DNAs, B1, B2, B3 and B4 was carried out. Restriction fragments of the nuclear DNAs hybridized with these plasmid-like DNAs showed polymorphisms in their length between Indica and Japonica rice cultivars. The hybridized signals found specifically in Indica or Japonica cultivars segregated in the F₂ population derived from a cross between these two subspecies. The observed ratio of the nuclear homologues in the F₂ population demonstrated that they were transmitted according to the Mendelian inheritance. The co-segregation of homologues was examined and the linkage was detected between the B1-nuclear homologue of Japonica and the B4-nuclear homologue of Indica, and also between the nuclear homologues of B2 and B3 of Indica. The linkage between the B1-nuclear homologue of Japonica and the B4-nuclear homologue of Indica was conserved in the different rice cultivars.

Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants (vol 65, pg 156, 2011)
Akira Kanazawa, J. Inaba, H. Shimura, S. Otagaki, S. Tsukahara, A. Matsuzawa, B. M. Kim, K. Goto, C. Masuta, PLANT JOURNAL, 90, 6, 1208, 1211, Jun. 2017
English, Others

Deep sequencing analysis reveals commonality in small RNA production between co-suppression and naturally occurring RNA silencing in petunia
Megumi Kasai, Hideo Matsumura, Kentaro Yoshida, Ryohei Terauchi, Akito Taneda, Akira Kanazawa, GENES & GENETIC SYSTEMS, 87, 6, 412, 412, Dec. 2012
English, Summary international conference

Tissue-specific promoter activity of the SORE-1 retrotransposon in soybean.
Tsuchiya M, Abe J, Kanazawa A, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 52, 5, 6, Dec. 2011
The Crop Science Society of Japan, Japanese

P-63 Development of Novel Miccanthus Breeding Materials through Genetic Modification for Highly Effident Biofuel Production
UKAJI Norifumi, WANG Xun, LI Xiaoyu, KANAZAWA Akira, YAMADA Toshihiko, Proceedings of the Conference on Biomass Science, 0, 6, 178, 179, 12 Jan. 2011
For control of global warning, it is proposed the utilizaiion of liquid biofuel produced from plant biomass. Perennial herbaceous plant, Miscanthus is a suitabis for the mateiial for biofuel because of a potential of massive-biomass production. However, it is necessaxy the further improvement of Miscanthus variety for efficient production of biofuel. Especially, low lignin contents Miscanthus cultiver will be greatly useful biomass materials that easily converted to biofuel. In the present study, we are attempting to reduce the lignin contents in Miscanthus by suppression of lignin synthesi..., The Japan Institute of Energy, Japanese

Modification of soybean seed components by gene silencing
金澤 章, 永松 敦, 井元 勇介, Soy protein research,Japan, 12, 30, 33, 39, Oct. 2009
不二たん白質研究振興財団, Japanese

A sterility between rice species S_<13^n>, a novel allele at the S_<13> locus that induces hybrid sterility between rice species
Ikegaya T, Koide Y, Sano Y, Kanazawa, 日本育種学会・日本作物学会北海道談話会会報, 49, 7, 8, Dec. 2008
The Crop Science Society of Japan, Japanese

Modification of soybean components through gene expression control by the function of RNA
永松 敦, 金澤 章, Soy protein research,Japan, 11, 29, 26, 31, Oct. 2008
不二たん白質研究振興財団, Japanese

(19)Induction of Methylation on the Promoter of an Endogene in Petunia Infected with Cucumber mosaic virus Vector(Abstracts Presented at the Meeting of the Hokkaido Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)
Inaba J., Tukahara S., Kanazawa A., Masuta C., Annals of the Phytopathological Society of Japan, 74, 1, 83, 83, 20 Feb. 2008
The Phytopathological Society of Japan (PSJ), Japanese

Hybrid male sterility induced by S13 found in a distantly related rice species
Yohei Koide, Tomohito Ikegaya, Daisuke Nishimoto, Akira Kanazawa, Yoshio Sano, GENES & GENETIC SYSTEMS, 82, 6, 522, 522, Dec. 2007
English, Summary international conference

The mRNA level of F3'H gene is associated with pigmentation in pubescence in soybean
Nagamatsu A, Masuta C, Matsuura H, Kitamura K, Abe J, Kanazawa A, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 48, 103, 104, Dec. 2007
The Crop Science Society of Japan, Japanese

Gene expression control of β-conglycinin in molecular breeding of soybean seed storage protein
井元 勇介, 永松 敦, 金澤 章, Soy protein research,Japan, 10, 28, 12, 17, Oct. 2007
不二たん白質研究振興財団, Japanese

Analysis of the gene expression control of soybean seed storage protein β-conglycinin α subunit
永松 敦, 金澤 章, Soy protein research,Japan, 9, 27, 30, 35, Oct. 2006
不二たん白質研究振興財団, Japanese

Analysis of the seed-specific transcriptional regulation of the soybean β-conglycinin α subunit gene
Nagamatsu A, Yoshino M, Tsutsumi K, Kanazawa A, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 46, 45, 46, Dec. 2005
The Crop Science Society of Japan, Japanese

Mechanisms for epigenetic regulation of pattern formation in petunia flower.
Koseki M, Goto K, Masuta C, Kanazawa A, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 45, 107, 108, Dec. 2004
The Crop Science Society of Japan, Japanese

ペレニアルライグラス (Lolium perenne L) におけるフルクタンネオシリーズおよびイヌリンを合成する酵素遺伝子の単離と同定
久野 裕, 金澤 章, 吉田 みどり, 中嶋 博, 喜多村 啓介, 山田 敏彦, 日本草地学会誌, 50, 0, 274, 275, 26 Mar. 2004
Japanese

Characterization of transgenic perennial ryegrass (Lolium perenne L.) carrying wheat (Triticum aestivum L.) genes encoding enzymes involved in fructan synthesis
H Hisano, A Kanazawa, A Kawakami, M Yoshida, T Yamada, Y Shimamoto, PLANT AND CELL PHYSIOLOGY, 44, S100, S100, 2003
English, Summary international conference

ダイズβ-コングリシニンのαサブユニットをコードする遺伝子の多様性とαサブユニットの発現(英文誌和文摘要)
吉野 道子, 金澤 章, 堤 賢一, 中村 郁郎, 高橋 浩司, 島本 義也, 育種学研究, 4, 4, 01 Dec. 2002
日本育種学会, Japanese

6-24 コムギ由来のフルクタン合成酵素をコードする遺伝子を導入したペレニアルライグラス (Lolium perenne L.) の特性
久野 裕, 金澤 章, 川上 顕, 吉田 みどり, 山田 敏彦, 中嶋 博, 阿部 純, 島本 義也, 日本草地学会誌, 48, 0, 350, 351, 21 Sep. 2002
日本草地学会, Japanese

DNA methylation and phenotypes of the progenies derived from crossing between PTGS line and TGS line in petunia
TAKAHASHI A., KANAZAWA A., 育種学研究 = Breeding research, 4, 118, 118, 26 Aug. 2002
Japanese

Production of transgenic perennial ryegrass (Lolium perenne L. ) carrying wheat genes encoding enzymes involved in fructan synthesis
HISANO H, KANAZAWA A, KAWAKAMI K, YOSHIDA M, YAMADA T, NAKASHIMA H, ABE J, SHIMAMOTO Y, 育種学研究 = Breeding research, 4, 0, 214, 214, 26 Aug. 2002
Japanese

Mapping of cold-induced genes in Lolium perenne L.
SHINOZUKA H, HISANO H, YONEYAMA S, KANAZAWA A, JONES E. S, FORSTER J. W, SHIMAMOTO Y, YAMADA T, 育種学研究 = Breeding research, 4, 0, 247, 247, 26 Aug. 2002
Japanese

Evolutionary dynamics of hybrid swarms between wild and cultivated soybeans
SAKAMOTO S, ABE J, KANAZAWA A, SHIMAMOTO Y, 育種学研究 = Breeding research, 4, 0, 420, 420, 26 Aug. 2002
Japanese

Induction of RNA silencing in limited cells of petals in petunia.
KANAZAWA A., 育種学研究 = Breeding research, 4, 1, 56, 56, 30 Mar. 2002
Japanese

Wild and Cultivated soybeans with common cytoplasmic DNA type differs in an allelic constitutions of nuclear SSRs.
SAKAMOTO S, ABE J, KANAZAWA A, SHIMAMOTO Y, 育種学研究 = Breeding research, 4, 1, 118, 118, 30 Mar. 2002
Japanese

Traits and origin of wild soybean having dominant cytoplasmic type of cultivated soybean
Sakamoto S, Abe J, Kanazawa A, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 42, 161, 162, Dec. 2001
The Crop Science Society of Japan, Japanese

Genetic relationship of soybean landraces assesd by SSR markers located on the same linkage groups
Suzuki Y, Abe J, Kanazawa A, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 41, 107, 108, Dec. 2000
The Crop Science Society of Japan, Japanese

Phylogenetic relationships of the genus Glycine inferred from sequence variations of chloroplast DNA
SAKAI M, XU D. H, KANAZAWA A, ABE J, SHIMAMOTO Y, 育種学研究 = Breeding research, 2, 2, 215, 215, 25 Sep. 2000
Japanese

Screening of markers linked with hard seedednessin soybean.
SAKAMOTO S, KANAZAWA A, ABE J, SHIMAMOTO Y, 育種学研究 = Breeding research, 2, 1, 43, 43, Apr. 2000
Japanese

Variation of nuclear RFLP markers between Chinese and Japanese Soybean populations.
SUZUKI Y, KANAZAWA A, ABE J, SHIMAMOTO Y, 育種学研究 = Breeding research, 2, 1, 256, 256, Apr. 2000
Japanese

Genetic diversity of soybean (G. max) germplasm in SSR markers
XU D. H, ABE J, KANAZAWA A, SHIMAMOTO Y, 育種学研究 = Breeding research, 2, 1, 257, 257, Apr. 2000
Japanese

Variation at four cpDNA mutation sites in wild and cultivated soybeans and its implication to the establishment of soybean.
ABE J, XU D. H, KANAZAWA A, SHIMAMOTO Y, 育種学研究 = Breeding research, 2, 1, 258, 258, Apr. 2000
Japanese

Genetic analysis of wild traits with wild soybean
Sakamoto S, Abe J, Kanazawa A, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 40, 93, 94, Dec. 1999
The Crop Science Society of Japan, Japanese

Genetic variation in Chinese and Japanese soybean (G. max) assessed by SSR markers
Xu D. H, Kanazawa A, Abe J, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 40, 95, 96, Dec. 1999
The Crop Science Society of Japan, English

Genetic difference among soybean landraces in China and Japan based on nuclear DNA polymorphisms
Suzuki Y, Abe J, Kanazawa A, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 40, 97, 98, Dec. 1999
The Crop Science Society of Japan, Japanese

Phylogenetic relationships in the genus Glycine based on sequence variations of chloroplast DNA
Sakai M, Kanazawa A, Abe J, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 40, 99, 100, Dec. 1999
The Crop Science Society of Japan, Japanese

3-2 ペレニアルライグラスにおける低温応答遺伝子群の発現および構造解析
富永 陽子, 金澤 章, 島本 義也, 日本草地学会誌, 45, 0, 110, 111, 20 Mar. 1999
日本草地学会, Japanese

Characterization of the seed storage proteins of the wild soybeans in Far East Russia
Yoshino M, Kanazawa A, Abe J, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 39, 103, 104, Dec. 1998
The Crop Science Society of Japan, Japanese

Genetic analysis of characters on evolution in wild soybean with hybrids distributed
Sakamoto S, Abe J, Kanazawa A, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 39, 105, 106, Dec. 1998
The Crop Science Society of Japan, Japanese

3-9 3'-RACE法の併用によるdifferential display法を利用したペレニアルライグラスにおける低温応答遺伝子の検出
富永 陽子, 金澤 章, 島本 義也, 日本草地学会誌, 44, 0, 122, 123, 25 Aug. 1998
日本草地学会, Japanese

Evoluationary relationships of genes for subunit of β-conglycinin in soybean based on the sequences of non-coding region
Yoshino M, Kanazawa A, Nakamura I, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 38, 106, 107, Dec. 1997
The Crop Science Society of Japan, Japanese

Soybean breeding and wild soybeans in Russia
Abe J, Kanazawa A, Komatsu K, Shimamoto Y, Shavrukov Y. N, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 38, 116, 117, Dec. 1997
The Crop Science Society of Japan, Japanese

Structure of the gene for e subunit of rice mitochondrial F_1-ATP synthase.
Kanazawa A, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 37, 16, 17, Dec. 1996
The Crop Science Society of Japan, Japanese

Rearrangement of the mitochondrial genome during evolutionary process of the genus Glycine
Tozuka A, Kato S, Kanazawa A, Abe J, Shimamoto Y, Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 0, 37, 146, 147, Dec. 1996
The Crop Science Society of Japan, Japanese