Dec. 2019, 農林水産省, 農業技術 2019年10大ニュース　　　　　　　　　　　　　　　
牛白血病の新たな制御方法、抗ウイルス効果の確認に成功 －牛の難治性疾病に対する応用に期待－
今内 覚

Jun. 2019, 伊藤記念財団, 第4回伊藤記念財団賞　　　　　　　　　　　　　　　
今内 覚

Jan. 2017, 北海道大学, 平成28年度北海道大学研究総長賞　　　　　　　　　　　　　　　
今内 覚

Sep. 2015, 日本獣医学会, 日本獣医学会賞　　　　　　　　　　　　　　　
Satoru Konnal

Feb. 2014, 北海道, 平成25年度北海道科学技術奨励賞　　　　　　　　　　　　　　　
今内 覚

Jun. 2005, 森永奉仕会, 森永奉仕会賞　　　　　　　　　　　　　　　
今内覚

Oct. 2003, 日本獣医学会, 獣医学奨励賞　　　　　　　　　　　　　　　
今内覚

Oct. 2003, 日本獣医学会, 日本獣医学会会長賞　　　　　　　　　　　　　　　
今内覚

Clinical efficacy of anti-PD-L1 antibody HFC-L1/c4G12 in dogs with malignant tumors: an exploratory study　　　　　　　　　　　　　　　
Takagi S., Tagawa M., Maekawa N., Konnai S., Kagawa Y., Hosoya K., Yamauchi A., Kudo A., Kamo S., Kim S., Kinoshita R., Deguchi T., Owaki R., Tachibana Y., Yokokawa M., Takeuchi H., Nakamura H., Kato Y., Kanazawa S., Abe T., Furuta T., Yamamoto K., Suzuki Y., Okagawa T., Murata S., Ohashi K.
J Vet Med Sci., 87, 12, 1419, 1425, Dec. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Enhancement of Vaccine-Induced T-cell Responses by Probiotics in Calves　　　　　　　　　　　　　　　
Ikehata M., Okagawa T., Nakamura H., Maekawa N., Suzuki Y., Murata D., Ohashi K., Konna S.
Vaccines, 13, 11, 1120, Oct. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Development of a multiplex real-time PCR assay for bovine immunodeficiency virus and bovine leukemia virus and its application to a large-scale survey in Kyushu, Japan　　　　　　　　　　　　　　　
Yamamoto M., Okagawa T., Konnai S., Mekata H.
J Vet Med Sci., 87, 10, 1164, 1169, Oct. 2025, [Peer-reviewed]
English, Scientific journal

Two distinct polymorphisms in the basic region of Meq protein of marek's disease virus alter pathological progression and clinical manifestations　　　　　　　　　　　　　　　
Sato J., Kurokawa A., Motai Y., Yamagami S., Win SY, Horio F., Saeki H., Maekawa N., Okagawa T., Kaufer BB, Osterrieder N., Parcells MS, Konnai S., Ohashi K., Murata S.
Virol J., 22, 1, 303, Sep. 2025, [Peer-reviewed]
English, Scientific journal

Genetic diversity and phylogenetic relationships among western and Asian honey bees, and Eristalis tenax collected in Myanmar　　　　　　　　　　　　　　　
Bawm S., Khaing A.P.P., Oo H.M., Mu M.M., Hmuu N.C., Zaw A.N.S., Lwin N., Chel H.M., Khaing Y., Win S.Y., Htun L.L., Murata S., Konnai S., Ohashi K., Nonaka N., Nakao R., Tun T. N.
J Asia Pac Entomol., in press, Sep. 2025, [Peer-reviewed]
English, Scientific journal

Rhipicephalus microplus triosephosphate isomerase dimer interface is stabilized by a key cysteine residue　　　　　　　　　　　　　　　
Saramago L., Cabrera N., Aguirre B., Gomes H., Moraes B., Itabajara da S.V., Marques M.A., Silva J.L., Okagawa T., Konnai S., Montfor R.P., Logullo C., Moraes J.
Biochim Biophys Acta Gen Subj., 1869, 12, 130857, Sep. 2025, [Peer-reviewed]
English, Scientific journal

Exploratory, randomized, dose-response study of the anti-PD-L1 antibody HFC-L1/c4G12 in dogs with pulmonary metastatic oral malignant melanoma　　　　　　　　　　　　　　　
Hosoya K., Kim S., Kinoshita R., Maekawa N., Konnai S., Takagi S., Tagawa M., Kagawa Y., Deguchi T., Owaki R., Tachibana Y., Yokokawa M., Takeuchi H., Nakamura H., Yamauchi A., Kudo A., Kamo S., Kato Y., Kanazawa S., Abe T., Furuta T., Yamamoto K., Suzuki Y., Okagawa T., Murata S., Ohashi K.
Vet Sci., 12, 9, 850., Sep. 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Evaluation of PD-L1 and TIM-3 pathways in T cells during experimental bovine leukemia virus infection in sheep　　　　　　　　　　　　　　　
Tiyamanee W., Okagawa T., Yamada S., Ikehata M., Nakamura H., Inoue M., Maekawa N., Kato Y., Murata S., Ohashi K., Murakami K., Konnai S.
Vet Sci., 12, 9, 810, Aug. 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Amino acid insertion in the Meq protein of Marek's disease virus, an avian oncogenic herpesvirus, accelerates tumorigenesis　　　　　　　　　　　　　　　
Sato J., Murata S., Motai Y., Win SY, Seo H., Yamagami S., Maekawa N., Okagawa T., Konnai S., Ohashi K.
Microbiol Spectr., 13, 8, e0336824, Aug. 2025, [Peer-reviewed]
English, Scientific journal

Development of Blood-Brain Barrier-Penetrating Antibodies for Neutralizing Tick-Borne Encephalitis Virus in the Brain　　　　　　　　　　　　　　　
Fukuta M., Fukano S., Maekawa N., Kobayashi S., Okamoto S., Hirano M., Nio-Kobayashi J., Kariwa H., Kawakami S., Konnai S., Yoshii K.
mSphere, 10, 7, e0018425., Jul. 2025, [Peer-reviewed]
English, Scientific journal

Amino Acid Polymorphisms in the Basic Region of Meq of Vaccine Strain CVI988 Drastically Diminish the Virulence of Marek's Disease Virus　　　　　　　　　　　　　　　
Sato J., Motai Y., Yamagami S., Kurokawa A., Win SY, Horio F., Saeki H., Maekawa N., Okagawa T., Konnai S., Ohashi K., Murata S.
Viruses, 17, 7, 907, Jun. 2025, [Peer-reviewed]
English, Scientific journal

Comprehensive Analysis of Immune Checkpoint Molecules Profiles Phenotype and Function of Exhausted T Cells in Enzootic Bovine Leukosis　　　　　　　　　　　　　　　
Nakamura H, Konnai S, Okagawa T, Maekawa N, Tiyamanee W, Ikehata M, Matsubara M, Watari K, Kamitani K, Saito M, Kato Y, Suzuki Y, Murata S, Ohashi K.
J. Immunol., 214, 10, 2600, 2616, 27 May 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Development of caninized anti-CTLA-4 antibody as salvage combination therapy for anti-PD-L1 refractory tumors in dogs　　　　　　　　　　　　　　　
Maekawa N., Konnai S., Watari K., Takeuchi H., Nakanishi T., Tachibana T., Hosoya K., Kim S., Kinoshita R., Owaki R., Yokokawa M., Kagawa Y., Takagi S., Deguchi T., Ohta, Kato Y., Yamamoto S., Yamamoto K., Suzuki Y., Okagawa T., Murata S., Ohashi K.
Front Immunol., 16, 1570717, 20 May 2025, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Genomic characteristics and distinctive genetic polymorphisms of Marek's disease virus strains circulating in Japan.
Shunsuke Yamagami, Jumpei Sato, Yoshinosuke Motai, Naoya Maekawa, Tomohiro Okagawa, Satoru Konnai, Kazuhiko Ohashi, Shiro Murata
The Journal of veterinary medical science, 87, 5, 517, 531, 15 May 2025, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Marek's disease virus (MDV) is an oncogenic herpesvirus that causes malignant lymphomas in chickens (Marek's disease, MD). Although MD is currently well controlled by vaccination, field strains of MDV tend to increase in virulence, and sporadic outbreaks have been reported worldwide, including in Japan. Previously, we determined the whole-genome sequence of a Japanese MDV strain, Kgs-c1, isolated in 2014, and analyzed its genetic characteristics. However, to date, Kgs-c1 is the only MDV strain that has been subjected to whole-genome sequencing, leaving the possibility that the key genetic features of the Japanese strains remain undiscovered. Therefore, we conducted whole-genome sequencing of two MDV strains, Kgs-c2 and Sg-c1, isolated in 2016 and 2019, respectively, and analyzed their genetic characteristics. Consistent with our previous findings, the genomic sequences of the Japanese strains were classified into the Eurasian cluster, suggesting that the Japanese strains are genetically closer to the European and Chinese strains than they are to the US strains. In contrast, the Japanese strains exhibited genetic features different from those of pathogenic European and Chinese strains. Furthermore, polymorphisms in the meq and infected cell protein 4 (ICP4) genes, which are candidates involved in the enhanced virulence of MDV, were observed among the newly isolated strains and Kgs-c1. Thus, three MDV strains isolated in Japan had different genetic features from those in other countries. These findings emphasize the necessity of periodic monitoring of MDV field strains in order to determine shifts in their characteristics and effectively control MD.

Performance evaluation of an improved RAISING method for clonality analysis of bovine leukemia virus-infected cells: a collaborative study in Japan　　　　　　　　　　　　　　　
Okagawa T., Nojiri N., Yoshida-Furihata H., Nao N., Tominaga M., Kohara J., Gondaira S., Higuchi H., Takeda Y., Ogawa H., Yamada S., Murakami K., Suzuki Y., Takai S., Maezawa M., Inokuma H., Shimizu K., Inoshima Y., Usui T., Tagawa M., Yamamoto M., Mekata H., Esaki M., Ozawa M., Matsudaira T., Maekawa N., Murata S., Ohashi K., Saito M., Konnai S.
J. Vet. Med. Sci., 87, 5, 551, 558, 15 May 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Programmed Cell Death-1 Expression in T-Cell Subsets in Chickens Infected with Marek's Disease Virus　　　　　　　　　　　　　　　
Sato J., Motai Y., Yamagami S., Win SY, Horio F., Saeki H., Maekawa N., Okagawa T., Konnai S., Ohashi K., Murata S.
Pathogens, 14, 5, 431, 29 Apr. 2025, [Peer-reviewed]
English, Scientific journal

Characterization of a lipocalin-like molecule from Dermanyssus gallinae as a potential vaccine antigen.
Fumiya Horio, Hikari Seo, Shwe Yee Win, Jumpei Sato, Yoshinosuke Motai, Shunsuke Yamagami, Takumi Sato, Eiji Ohishi, Naoya Maekawa, Tomohiro Okagawa, Satoru Konnai, Kazuhiko Ohashi, Shiro Murata
Veterinary immunology and immunopathology, 283, 110921, 110921, 24 Mar. 2025, [International Magazine]
English, Scientific journal, Poultry red mites (PRMs, Dermanyssus gallinae) are hematophagous ectoparasites of chickens that pose a significant threat to the egg-laying industry. The emergence of acaricide-resistant PRMs raises the demand for alternative control approaches such as vaccination. However, several vaccine antigens have failed to suppress the growth of PRM populations in field trials due to difficulties in maintaining antibody levels. In ticks, the molecules exposed to the host, such as lipocalins, can facilitate antibody production, and are therefore considered advantageous as vaccine antigens. Therefore, we focused on a lipocalin-like molecule (Dg-Lipocalin) identified from an RNA-seq analysis reported by Fujisawa et al. (2020) and analyzed its exposure to the host and potential as a vaccine antigen. Western blotting using 500-fold diluted plasma of chickens from PRM-contaminated farms revealed the presence of antibodies against Dg-Lipocalin, suggesting its exposure to the host. To evaluate its potential as a vaccine antigen, PRMs were artificially fed immune plasma with 32,000- to 64,000-fold antibody titers or plasma from PBS-inoculated control chickens, and their mortality was observed for 7 days. In experiment 1, the immune plasma significantly increased PRM mortality compared to the control plasma. However, these effects were not observed in experiment 2, although the total mortality was significantly increased in immune plasma-fed PRMs. Thus, the efficacy of Dg-Lipocalin appears to be limited; however, its exposure to the host may result in sustained antibody titers. Further investigation is required to evaluate its feasibility.

Enhancement of radio-sensitivity by inhibition of Janus kinase signaling with oclacitinib in canine tumor cell lines.
Ryo Owaki, Kenji Hosoya, Tatsuya Deguchi, Satoru Konnai, Naoya Maekawa, Tomohiro Okagawa, Hironobu Yasui, Sangho Kim, Takafumi Sunaga, Masahiro Okumura
Molecular therapy. Oncology, 33, 1, 200946, 200946, 20 Mar. 2025, [International Magazine]
English, Scientific journal, A combination of irradiation and oclacitinib, a Janus kinase (JAK) inhibitor used in dogs, could lead to synergistic anticancer effects in canine tumors. However, the anti-tumor effects of oclacitinib remain unclear. This study investigated the radio-sensitizing effect of oclacitinib in canine tumors and determined its underlying mechanisms using osteosarcoma (HMPOS), malignant melanoma (CMeC), and thyroid adenocarcinoma (CTAC) cell lines. A clonogenic assay and a tumor growth assessment in a xenograft mouse model (BALB/cAJcl-nu/nu) were performed to evaluate the radio-sensitizing effects of oclacitinib. Oclacitinib enhanced the radio-sensitivity of tumor cells both in vitro and in vivo. The signal transducer and activator of transcription (STAT)3 expression was activated and suppressed by oclacitinib in X-irradiation-exposed cells. Oclacitinib enhanced radiation-induced apoptosis only in HMPOS cells by inhibiting anti-apoptotic genes. In addition, oclacitinib inhibited the transcription of cell-cycle-regulating genes and arrested cell cycle progression from the G1 phase to subsequent phases. In conclusion, oclacitinib enhanced radio-sensitivity both in vitro and in vivo by triggering apoptosis and impeding cell cycle progression via STAT3 inhibition in canine tumor cell lines. This study suggested the clinical therapeutic potential of oclacitinib and radiation therapy in enhancing treatment efficacy and outcomes in canine tumors.

Host Skin during Tick Infestation: Unearthing the Local Immunity to Lead an Anti-Tick Vaccine　　　　　　　　　　　　　　　
Joao BC, Parizi LF, Zhou J., Konnai S., Termignoni C., da Silva Vaz I J.
Acta Scientiae Veterinariae, 53, 1982, Mar. 2025, [Peer-reviewed]
English, Scientific journal

Potential of histamine release factor for the utilization as a universal vaccine antigen against poultry red mites, tropical fowl mites, and northern fowl mites.
Shwe Yee Win, Fumiya Horio, Jumpei Sato, Yoshinosuke Motai, Hikari Seo, Sotaro Fujisawa, Takumi Sato, Eiji Oishi, Lat Lat Htun, Saw Bawm, Tomohiro Okagawa, Naoya Maekawa, Satoru Konnai, Kazuhiko Ohashi, Shiro Murata
The Journal of veterinary medical science, 87, 1, 1, 12, 01 Jan. 2025, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Poultry red mites (PRMs, Dermanyssus gallinae), tropical fowl mites (TFMs, Ornithonyssus bursa), and northern fowl mites (NFMs, Ornithonyssus sylviarum) are hematophagous mites that are distributed worldwide which pose a serious challenge to the poultry industry and negatively impact poultry production and welfare. Vaccines represent a promising approach for controlling avian mites, and the identification of antigens with broad efficacy against multiple avian mite species is advantageous for vaccine control. This study aimed to identify histamine release factor (HRF), which was previously reported as a candidate vaccine antigen against PRMs, from TFMs and NFMs and to analyze its cross-reactivity and acaricidal effects on different avian mite species. The deduced amino acid sequences of the HRFs identified in the TFMs and NFMs were highly homologous to those of the PRMs. We generated recombinant HRF (rHRF) of TFMs, NFMs, and PRMs, and immune plasma against each rHRF was produced by immunization with each antigen. The immune plasma contained antibodies specific to each antigen and showed cross-reactivity with rHRFs from different avian mites. Moreover, PRM nymphs (protonymphs) artificially fed each immune plasma showed higher mortality rates than those fed the control plasma. These results suggest that HRFs can be used as candidate antigens for a universal vaccine with broad efficacy across avian mites.

Starvation Metabolism Adaptations in Tick Embryonic Cells BME26　　　　　　　　　　　　　　　
Nogueira CL, Arcanjo AF, Lima ME, Moraes B., da Silva RM, Gondim KC, Konnai S., Ramos I., Santos S., Filardy AD, Pinto KG, Vaz Junior IDS, Logullo C.
Int J Mol Sci., 26, 1, 87, Dec. 2024, [Peer-reviewed]
English

Effect of C-to-T transition at CpG sites on tumor suppressor genes in tumor development in cattle evaluated by somatic mutation analysis in enzootic bovine leukosis　　　　　　　　　　　　　　　
Nishimori A, Andoh K, Matsuura Y, Okagawa T, Konnai S
mSphere, e0021624, Oct. 2024, [Peer-reviewed], [Last author]
English, Scientific journal

子牛における地方病性牛伝染性リンパ腫発症は母牛由来の腫瘍細胞が原因か?　　　　　　　　　　　　　　　
廣瀬 海凪, 今内 覚, 岡川 朋弘, 富永 みその, 松原 幸芽, 前川 直也, 村田 史郎, 大橋 和彦
北海道獣医師会雑誌, 68, 8, 278, 278, (公社)北海道獣医師会, Aug. 2024
Japanese

An Ixodes persulcatus inhibitor of plasmin and thrombin hinders keratinocyte migration, blood coagulation, and endothelial permeability.　　　　　　　　　　　　　　　
Berger M, Rosa da Mata S, Pizzolatti NM, Parizi LF, Konnai S, da Silva Vaz I Jr, Seixas A, Tirloni L
J Invest Dermatol., 144, 5, 1112, 1123, May 2024, [Peer-reviewed]
English

Chimeric provirus of bovine leukemia virus/ SMAD family member 3 in cattle with enzootic bovine leukosis　　　　　　　　　　　　　　　
Nao N, Okagawa T, Nojiri N, Konnai S, Shimakura H, Tominaga M, Yoshida-Frihata H, Nishiyama E, Matsudaira T, Maekawa N, Murata S, Muramatsu M, Ohashi K, Saito M
Archi Virol., 169, 3, 47, Feb. 2024, [Peer-reviewed]
English, Scientific journal

In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model　　　　　　　　　　　　　　　
Win SY, Seo H, Horio F, Fujisawa S, Sato J, Motai Y, Sato T, Oishi E, Taneno A, Htun LL, Bawn S, Okagawa T, Maekawa N, Konnai S, Ohashi K, Murata S
Vaccines (Basel), 12, 2, 148, Jan. 2024, [Peer-reviewed]
English, Scientific journal

Characterization of a Very Short Meq Protein Isoform in a Marek's Disease Virus Strain in Japan　　　　　　　　　　　　　　　
Motai Y, Murata S, Sato J, Nishi A, Maekawa N, Okagawa T, Konnai S, Ohashi K
Vet Sci., 11, 1, 43, Jan. 2024, [Peer-reviewed]
English, Scientific journal

G6PDH as a key immunometabolic and redox trigger in arthropods　　　　　　　　　　　　　　　
Moraes B, Martins R, Lopes C, Martins R, Arcanjo A, Nascimento J, Konnai S, da Silva Vaz I Jr, Logullo C
Front Physiol., 14, 1287090, Nov. 2023, [Peer-reviewed]
English, Scientific journal

Safety and clinical efficacy of an anti-PD-L1 antibody (c4G12) in dogs with advanced malignant tumours
Maekawa N, Konnai S, Hosoya K, Kim S, Kinoshita R, Deguchi T, Owaki R, Tachibana Y, Yokokawa M, Takeuchi H, Kagawa Y, Takagi S, Ohta H, Kato Y, Yamamoto S, Yamamoto K, Suzuk Y, Okagawa T, Murata S, Ohashi K
PLOS ONE, 18, 10, e0291727, Oct. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Immune checkpoint inhibitors (ICIs) have been developed for canine tumour treatment, and pilot clinical studies have demonstrated their antitumour efficacy in dogs with oral malignant melanoma (OMM). Although ICIs have been approved for various human malignancies, their clinical benefits in other tumour types remain to be elucidated in dogs. Here, we conducted a clinical study of c4G12, a canine chimeric anti-PD-L1 antibody, to assess its safety and efficacy in dogs with various advanced malignant tumours (n = 12) at the Veterinary Teaching Hospital of Hokkaido University from 2018 to 2023. Dogs with digit or foot pad malignant melanoma (n = 4), osteosarcoma (n = 2), hemangiosarcoma (n = 1), transitional cell carcinoma (n = 1), nasal adenocarcinoma (n = 1), B-cell lymphoma (n = 1), or undifferentiated sarcoma (n = 2) were treated with 2 or 5 mg/kg c4G12 every 2 weeks. Treatment-related adverse events of any grade were observed in eight dogs (66.7%), including elevated aspartate aminotransferase (grade 3) in one dog (8.3%) and thrombocytopenia (grade 4) in another dog (8.3%). Among dogs with target disease at baseline (n = 8), as defined by the response evaluation criteria for solid tumours in dogs (cRECIST), one dog with nasal adenocarcinoma and another with osteosarcoma experienced a partial response (PR), with an objective response rate of 25.0% (2 PR out of 8 dogs; 95% confidence interval: 3.2-65.1%). These results suggest that c4G12 is safe and tolerable and shows antitumor effects in dogs with malignant tumours other than OMM. Further clinical studies are warranted to identify the tumour types that are most likely to benefit from c4G12 treatment.

Development of a high-affinity anti-bovine PD-1 rabbit–bovine chimeric antibody using an efficient selection and large production system
Okagawa T, Konnai S, Goto S, Sajiki Y, Ganbaatar O, Watari K, Nakamura H, Wang CX, Tachibana T, Kato Y, Kameda Y, Kohara J, Terasaki N, Kubota M, Takeda A, Takahashi H, Suzuki Y, Maekawa N, Murata S, Ohashi K
Vet Res., 54, 1, 82, 82, Sep. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Immune checkpoint molecules PD-1/PD-L1 cause T-cell exhaustion and contribute to disease progression in chronic infections of cattle. We established monoclonal antibodies (mAbs) that specifically inhibit the binding of bovine PD-1/PD-L1; however, conventional anti-PD-1 mAbs are not suitable as therapeutic agents because of their low binding affinity to antigen. In addition, their sensitivity for the detection of bovine PD-1 is low and their use for immunostaining PD-1 is limited. To address these issues, we established two anti-bovine PD-1 rabbit mAbs (1F10F1 and 4F5F2) and its chimeric form using bovine IgG1 (Boch1D10F1), which exhibit high binding affinity. One of the rabbit mAb 1D10F1 binds more strongly to bovine PD-1 compared with a conventional anti-PD-1 mAb (5D2) and exhibits marked inhibitory activity on the PD-1/PD-L1 interaction. In addition, PD-1 expression in bovine T cells could be detected with higher sensitivity by flow cytometry using 1D10F1. Furthermore, we established higher-producing cells of Boch1D10F1 and succeeded in the mass production of Boch1D10F1. Boch1D10F1 exhibited a similar binding affinity to bovine PD-1 and the inhibitory activity on PD-1/PD-L1 binding compared with 1D10F1. The immune activation by Boch1D10F1 was also confirmed by the enhancement of IFN-γ production. Finally, Boch1D10F1 was administered to bovine leukemia virus-infected cows to determine its antiviral effect. In conclusion, the high-affinity anti-PD-1 antibody developed in this study represents a powerful tool for detecting and inhibiting bovine PD-1 and is a candidate for PD-1-targeted immunotherapy in cattle.

Characterization of cysteine proteases from poultry red mite, tropical fowl mite, and northern fowl mite to assess the feasibility of developing a broadly efficacious vaccine against multiple mite species
Win SY, Murata S, Fujisawa S, Seo H, Sato J, Motai Y, Sato T, Oishi E, Taneno A, Htun LL, Bawm S, Okagawa T, Maekawa N, Konnai S, Ohashi K
PLoS One, 18, 7, e0288565, Jul. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Infestation with poultry red mites (PRM, Dermanyssus gallinae) causes anemia, reduced egg production, and death in serious cases, resulting in significant economic losses to the poultry industry. As a novel strategy for controlling PRMs, vaccine approaches have been focused upon and several candidate vaccine antigens against PRMs have been reported. Tropical (TFM, Ornithonyssus bursa) and northern (NFM, Ornithonyssus sylviarum) fowl mites are also hematophagous and cause poultry industry problems similar to those caused by PRM. Therefore, ideal antigens for anti-PRM vaccines are molecules that cross-react with TFMs and NFMs, producing pesticidal effects similar to those against PRMs. In this study, to investigate the potential feasibility of developing vaccines with broad efficacy across mite species, we identified and characterized cysteine proteases (CPs) of TFMs and NFMs, which were previously reported to be effective vaccine antigens of PRMs. The open reading frames of CPs from TFMs and NFMs had the same sequences, which was 73.0% similar to that of PRMs. Phylogenetic analysis revealed that the CPs of TFMs and NFMs clustered in the same clade as CPs of PRMs. To assess protein functionality, we generated recombinant peptidase domains of CPs (rCP-PDs), revealing all rCP-PDs showed CP-like activities. Importantly, the plasma obtained from chickens immunized with each rCP-PD cross-reacted with rCP-PDs of different mites. Finally, all immune plasma of rCP-PDs reduced the survival rate of PRMs, even when the plasma was collected from chickens immunized with rCP-PDs derived from TFM and NFM. Therefore, CP antigen is a promising, broadly efficacious vaccine candidate against different avian mites.

Enhanced systemic antitumour immunity by hypofractionated radiotherapy and anti-PD-L1 therapy in dogs with pulmonary metastatic oral malignant melanoma
Deguchi T, Maekawa N, Konnai S, Owaki R, Hosoya K, Morishita K, Nakamura M, Okagawa T, Takeuchi H, Kim S, Kinoshita R, Tachibana Y, Yokokawa M, Takagi S, Kato Y, Suzuk Y, Murata S, Ohashi K
Cancers, 15, 11, 3013, Jun. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Although immune checkpoint inhibitors (ICIs), such as the anti-programmed death-ligand 1 (PD-L1) antibody, have been developed for the treatment of canine malignant melanoma, desirable clinical efficacies have not been achieved. Recent studies in humans have suggested that radiation therapy (RT) combined with ICIs induces robust systemic antitumour immunity in patients with cancer. This study retrospectively examined the therapeutic efficacy of combination therapy (hypofractionated RT and anti-PD-L1 antibody [c4G12]) in dogs with pulmonary metastatic oral malignant melanoma. The intrathoracic clinical benefit rate (CBR)/median overall survival (OS) in the no RT (n = 20, free from the effect of RT), previous RT (n = 9, received RT ≤8 weeks prior to the first c4G12 dose), and concurrent RT (n = 10, c4G12 therapy within ±1 week of the first RT fraction) groups were 10%/185 days, 55.6%/283.5 days (p < 0.05 vs. no RT group), and 20%/129 days (p > 0.05 vs. no RT group), respectively. The adverse events were considered to be tolerable in the combination therapy. Thus, hypofractionated RT before the initiation of c4G12 therapy can be an effective approach for enhancing the therapeutic efficacy of immunotherapy, with acceptable safety profiles. Further prospective clinical studies are required to confirm the findings of this study.

Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in sheep
Tiyamanee W, Konnai S, Okagawa T, Nojima Y, Ganbaatar O, Maekawa N, Hasebe R, Kagawa Y, Kato Y, Suzuki Y, Murata S, Ohashi K
Vet Immunol Immunopathol., 11, 261, 110609, 110609, May 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Sheep have been used as a large animal experimental model for studying infectious diseases. However, due to a lack of staining antibodies and reagents, immunological studies on sheep have not progressed. The immunoinhibitory receptor programmed death-1 (PD-1) is expressed on T lymphocytes. The interaction of PD-1 with its ligand PD-ligand 1 (PD-L1) delivers inhibitory signals and impairs proliferation, cytokine production, and cytotoxicity of T cells. We previously reported that the PD-1/PD-L1 pathway was closely associated with T-cell exhaustion and disease progression in bovine chronic infections using anti-bovine PD-L1 monoclonal antibodies (mAbs). Furthermore, we found that blocking antibodies against PD-1 and PD-L1 restore T-cell functions and could be used in immunotherapy of cattle. However, the immunological role of the PD-1/PD-L1 pathway in chronic diseases of sheep remains unknown. In this study, we identified cDNA sequences of ovine PD-1 and PD-L1 and examined the cross-activity of anti-bovine PD-L1 mAbs against ovine PD-L1 as well as the expression of PD-L1 in ovine listeriosis. The amino acid sequences of ovine PD-1 and PD-L1 share a high degree of identity and similarity with homologs from ruminants and other mammalian species. Anti-bovine PD-L1 mAb recognized ovine PD-L1 on lymphocytes in the flow cytometric assay. Furthermore, an immunohistochemical staining confirmed the PD-L1 expression on macrophages in the brain lesions of ovine listeriosis. These findings indicated that our anti-PD-L1 mAb would be useful for analyzing the ovine PD-1/PD-L1 pathway. Further research is needed to determine the immunological role of PD-1/PD-L1 in chronic diseases such as BLV infection through experimental infection of sheep.

Analysis of gene expression in poultry red mite, Dermanyssus gallinae, by RNAscope in situ hybridization
Seo H, Murata S, Ichii O, Namba T, Win SY, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Konnai S, Ohashi K
J Vet Med Sci., 85, 5, 532, 535, May 2023, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The poultry red mite (PRM; Dermanyssus gallinae) is a hematophagous ectoparasite that mainly infests chickens, and its infestation causes significant economic losses to the poultry industry. In this study, we examined the use of RNAscope-based in situ hybridization (ISH) to characterize gene expression in PRM. We analyzed the mRNA expression of Dermanyssus gallinaecathepsin D-1 (Dg-CatD-1) and Dermanyssus gallinae cystatin (Dg-Cys). RNAscope ISH analysis revealed that mRNA expression of Dg-CatD-1 was observed in the digestive tract, and Dg-Cystatin mRNA was expressed in the ovaries in addition to the digestive tract. RNAscope ISH could be applicable for the analysis of gene expression in each tissue of PRM and is an effective method to investigate the characteristics of target genes.

Investigation of peripheral blood responses in chickens infested with Dermanyssus gallinae
Fujisawa S, Murata S, Sato T, Oishi E, Taneno A, Konnai S, Ohashi K
Parasitol Int., 95, 102754, 102754, Apr. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Among haematophagous ectoparasites that infest chickens, poultry red mite (Dermanyssus gallinae, PRM) is one of the most serious threats to poultry farms. Mass PRM infestation causes various health problems in chickens, resulting in significant productivity reduction in the poultry industry. Despite the efficiency of acaricides for controlling PRMs, the emergence of acaricide-resistant PRMs represents a challenging setback. Infestation with haematophagous ectoparasites, such as PRMs, induces inflammatory and haemostatic reactions in the host. Therefore, we aimed to explore the gene expression in chicken peripheral blood cells to elucidate host responses against PRM infestation in detail. RNA sequencing of blood-fed PRMs was performed, and the levels of the chicken-derived transcripts obtained from the ingested blood cells were analysed. Genes encoding haemoglobin subunits were found to be significantly more expressed, suggesting that PRM infestation causes anaemia in chickens. Additionally, the mRNA and plasma concentrations of CC chemokine ligand 4 and β2 microglobulin among the immune-related molecules were found to be significantly higher in PRM-infested chickens compared with non-infested animals. These results suggest that PRM infestation induce inflammation in chicken. Further studies are warranted to better understand the influence of PRM infestation on the host physiological states, including immunity.

Potential of ferritin 2 as an antigen for the development of a universal vaccine for avian mites, poultry red mites, tropical fowl mites, and northern fowl mites
Win SY, Murata S, Fujisawa S, Seo H, Sato J, Motai Y, Sato T, Oishi E, Taneno A, Htun LL, Bawn S, Okagawa T, Maekawa N, Konnai S, Ohashi K
Front Vet Sci., 10, 1182930, 1182930, Apr. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, INTRODUCTION: Poultry red mites (PRMs, Dermanyssus gallinae), blood-sucking ectoparasites, are a threat to the poultry industry because of reduced production caused by infestation. In addition, tropical fowl mites (TFMs, Ornithonyssus bursa) and northern fowl mites (NFMs, Ornithonyssus sylviarum) are hematophagous, distributed in various regions, genetically and morphologically close to PRMs, and cause similar problems to the poultry industry. Vaccine approaches have been studied for PRM control, and several molecules have been identified in PRMs as candidates for effective vaccine antigens. The development of an anti-PRM vaccine as a universal vaccine with broad efficacy against avian mites could improve the productivity of poultry farms worldwide. Molecules that are highly conserved among avian mites and have critical functions in the physiology and growth of mites could be ideal antigen candidates for the development of universal vaccines. Ferritin 2 (FER2), an iron-binding protein, is critical for the reproduction and survival of PRMs and has been reported as a useful vaccine antigen for the control of PRMs and a candidate for the universal vaccine antigen in some tick species. METHOD AND RESULTS: Herein, we identified and characterized FER2 in TFMs and NFM. Compared with the sequence of PRM, the ferroxidase centers of the heavy chain subunits were conserved in FER2 of TFMs and NFMs. Phylogenetic analysis revealed that FER2 belongs to clusters of secretory ferritins of mites and other arthropods. Recombinant FER2 (rFER2) proteins from PRMs, TFMs, and NFMs exhibited iron-binding abilities. Immunization with each rFER2 induced strong antibody responses in chickens, and each immune plasma cross-reacted with rFER2 from different mites. Moreover, mortality rates of PRMs fed with immune plasma against rFER2 from TFMs or NFMs, in addition to PRMs, were higher than those of control plasma. DISCUSSION: rFER2 from each avian mite exhibited anti-PRM effects. This data suggests that it has the potential to be used as an antigen candidate for a universal vaccine against avian mites. Further studies are needed to access the usefulness of FER2 as a universal vaccine for the control of avian mites.

In vitro evaluation of Lactiplantibacillus plantarum HOKKAIDO strain, effective lactic acid bacteria for calf diarrhea
Ikehata M, Konnai S, Okagawa T, Abe K, Honma M, Kitamura T, Maekawa N, Suzuki Y, Murata S, Ohashi K
Front Vet Sci., 10, 1145445, Apr. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Calf diarrhea adversely affects growth and sometimes results in mortality, leading to severe economic losses to the cattle industry. Antibiotics are useful in the treatment against bacterial diarrhea, but not against viral, protozoan, and antibiotic-resistant bacterial diarrhea. Therefore, there are growing requirements for a novel control method for calf diarrhea. Probiotics have been considered promising candidates for preventive and supportive therapy for calf diarrhea for many years. A recent study has revealed that Lactiplantibacillus plantarum HOKKAIDO strain (Lp-HKD) reduces intestinal pathology and the severity of diarrhea in bovine rotavirus (BRV)-infected calves. Lp-HKD is known to enhance the function of human immune cells and expected to be used as probiotics for humans. Therefore, it is hypothesized that Lp-HKD modulates antiviral immune response in cattle and provide the clinical benefits in BRV-infected calves. However, the detailed mechanism of Lp-HKD-induced immunomodulation remains unknown. Thus, this study aimed to elucidate the immunomodulatory and antiviral effects of Lp-HKD in cattle. Cultivation assay of bovine peripheral blood mononuclear cells (PBMCs) showed that live and heat-killed Lp-HKD stimulates the production of interleukin-1β (IL-1β), IL-6, IL-10, and interferon-γ (IFN-γ) from PBMCs. Stimulation by heat-killed Lp-HKD yielded stronger cytokine production than stimulation by the live Lp-HKD. Additionally, CD14+ monocytes were identified as major producers of IL-1β, IL-6, and IL-10 under Lp-HKD stimulation; however, IFN-γ was mainly produced from immune cells other than CD14+ monocytes. Depletion of CD14+ monocytes from the PBMCs cultivation strongly decreased cytokine production induced by heat-killed Lp-HKD. The inhibition of toll-like receptor (TLR) 2/4 signaling decreased IL-1β and IL-6 production induced by live Lp-HKD and IL-1β, IL-6, and IFN-γ production induced by heat-killed Lp-HKD. Furthermore, live or heat-killed Lp-HKD also activated T cells and their production of IFN-γ and tumor necrosis factor-α. Then, culture supernatants of bovine PBMCs treated with heat-killed Lp-HKD demonstrated antiviral effects against BRV in vitro. In conclusion, this study demonstrated that Lp-HKD activates the functions of bovine immune cells via TLR2/4 signaling and exerts an antiviral effect against BRV through the induction of antiviral cytokines. Lp-HKD could be useful for the prevention and treatment of calf diarrhea through its immune activating effect.

Enhancement of vaccine-induced T-cell responses by PD-L1 blockade in calves
Okagawa T, Konnai S, Nakamura H, Ganbaatar O, Sajiki Y, Watari K, Noda H, Honma M, Kato Y, Suzuki Y, Maekawa N, Murata S, Ohashi K
Vaccines, 11, 3, 559, Mar. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Interactions between programmed death 1 (PD-1) and PD-ligand 1 (PD-L1) cause functional exhaustion of T cells by inducing inhibitory signals, thereby attenuating effector functions of T cells. We have developed an anti-bovine PD-L1 blocking antibody (Ab) and have demonstrated that blockade of the interaction between PD-1 and PD-L1 reactivates T-cell responses in cattle. In the present study, we examined the potential utility of PD-1/PD-L1-targeted immunotherapy in enhancing T-cell responses to vaccination. Calves were inoculated with a hexavalent live-attenuated viral vaccine against bovine respiratory infections in combination with treatment with an anti-PD-L1 Ab. The expression kinetics of PD-1 in T cells and T-cell responses to viral antigens were measured before and after vaccination to evaluate the adjuvant effect of anti-PD-L1 Ab. PD-1 expression was upregulated in vaccinated calves after the administration of a booster vaccination. The activation status of CD4+, CD8+, and γδTCR+ T cells was enhanced by the combination of vaccination and PD-L1 blockade. In addition, IFN-γ responses to viral antigens were increased following combinatorial vaccination with PD-L1 blockade. In conclusion, the blockade of the PD-1/PD-L1 interaction enhances T-cell responses induced by vaccination in cattle, indicating the potential utility of anti-PD-L1 Ab in improving the efficacy of current vaccination programs.

Regulation of programmed death ligand 1 expression by interferon-γ and tumour necrosis factor-α in canine tumour cell lines
Owaki R, Deguchi T, Konnai S, Maekawa N, Okagawa T, Hosoya K, Kim S, Sunaga T, Okumura M
Vet Comp Oncol., 1, 12, 21 Feb. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, Expression of programmed death ligand 1 (PD-L1) on tumor cells provides an immune evasion mechanism by inducing suppression of cytotoxic T cells. Various regulatory mechanisms of PD-L1 expression have been described in human tumours, however, little is known in canine tumours. To investigate whether inflammatory signaling is involved in PD-L1 regulation in canine tumours, the effects of interferon (IFN)-γ and tumour necrosis factor (TNF)-α treatment were examined in canine malignant melanoma cell lines (CMeC and LMeC) and an osteosarcoma cell line (HMPOS). The protein level of PD-L1 expression was upregulated by IFN-γ and TNF-α stimulation. Upon IFN-γ stimulation, all cell lines showed an increase in expression of PD-L1, signal transducer and activator of transcription (STAT)1, STAT3 and genes regulated by STAT activation. Upregulated expression of these genes was suppressed by the addition of a JAK inhibitor, oclacitinib. Contrastingly, upon TNF-α stimulation, all cell lines exhibited higher gene expression of the nuclear factor kappa B (NF-κB) gene RELA and genes regulated by NF-κB activation, whereas expression of PD-L1 was upregulated in LMeC only. Upregulated expression of these genes was suppressed by the addition of an NF-κB inhibitor, BAY 11-7082. The expression level of cell surface PD-L1 induced by IFN-γ and TNF-α treatment was reduced by oclacitinib and BAY 11-7082, respectively, indicating that upregulation of PD-L1 expression by IFN-γ and TNF-α stimulation is regulated via the JAK-STAT and NF-κB signaling pathways, respectively. These results provide insights into the role of inflammatory signaling in PD-L1 regulation in canine tumours. This article is protected by copyright. All rights reserved.

Suppressive modulation of host immune responses by Dermanyssus gallinae infestation
Fujisawa S, Murata S, Isezaki M, Win SY, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Konnai S, Ohashi K
Poult Sci., 102, 4, 102532, 102532, Feb. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, The poultry red mite (Dermanyssus gallinae, PRM) is a blood-sucking ectoparasite in chickens and is one of the most serious threats to poultry farms. Mass infestation with PRMs causes various health problems in chickens, resulting in significant productivity reduction in the poultry industry. Infestation with hematophagous ectoparasites, such as ticks, induces host inflammatory and hemostatic reactions. On the other hand, several studies have reported that hematophagous ectoparasites secrete various immunosuppressants from their saliva to suppress host immune responses to maintain blood sucking. Here, we examined the expression of cytokines in peripheral blood cells to investigate whether PRM infestation affects immunological states in chickens. In PRM-infested chickens, anti-inflammatory cytokines, IL-10 and TGF-β1, and immune checkpoint molecules, CTLA-4 and PD-1, were highly expressed compared to noninfested chickens. PRM-derived soluble mite extracts (SME) upregulated the gene expression of IL-10 in peripheral blood cells and HD-11 chicken macrophages. In addition, SME suppressed the expression of interferons and inflammatory cytokines in HD-11 chicken macrophages. Moreover, SME induces the polarization of macrophages into anti-inflammatory phenotypes. Collectively, PRM infestation could affect host immune responses, especially suppress the inflammatory responses. Further studies are warranted to fully understand the influence of PRM infestation on host immunity.

Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies
Takeuchi H, Nakajima C, Konnai S, Maekawa N, Okagawa T, Usui M, Tamura U, Suzuki Y, Murata S, Ohashi K
PLOS One, 18, 1, e0281171, Jan. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Coagulase-positive Staphylococci express protein A, which binds to host antibodies, to evade the immune system. Taking advantage of its specific binding to antibodies, protein A from Staphylococcus aureus, which is called SpA, is commonly used as an affinity chromatography ligand for human therapeutic antibodies. However, among four canine IgG subclasses (A, B, C, and D), only IgG-B binds to SpA strongly and establishing an efficient and robust purification scheme for canine therapeutic antibodies whose IgG subclass is A, C, or D remains difficult and depends on finding a suitable substitute to SpA. S. pseudintermedius, a major coagulase-positive Staphylococci found in dogs, expresses spsQ gene which is orthologous to S. aureus spa. We hypothesized that to serve S. pseudintermedius to better adapt to the dog immune system, SpsQ would bind to canine IgGs stronger than SpA, making it a better affinity chromatography ligand for canine therapeutic antibodies. To characterize SpsQ, we first determined the spsQ nucleotide sequence from S. pseudintermedius isolates. Based on the identified sequence, we prepared recombinant proteins containing the immunoglobulin-binding domains of SpA (r-SpA) and SpsQ (r-SpsQ) and determined their binding capacity for each canine IgG subclass. The binding capacity of r-SpsQ for IgG-B was almost as high as that of r-SpA. Interestingly, while both r-SpsQ and r-SpA showed no binding to IgG-C, the binding capacity of r-SpsQ for IgG-A and IgG-D was significantly higher than that of r-SpA. Finally, we performed affinity chromatography using r-SpsQ- or r-SpA-immobilized resin and revealed that the recovery rates of IgG-A and IgG-D using r-SpsQ were significantly higher than those using r-SpA. Our findings indicate that SpsQ has a strong potential to be used as an affinity chromatography ligand for canine therapeutic antibodies of subclass A, B, and D.

Molecular characterization of feline immune checkpoint molecules and establishment of PD-L1 immunohistochemistry for feline tumors
Maekawa N, Konnai S, Asano Y, Otsuka T, Aoki E, Takeuchi H, Kato Y, Kaneko M, Yamada S, Kagawa Y, Nishimura M, Takagi S, Deguchi T, Ohta H, Nakagawa T, Suzuki Y, Okagawa T, Murata S, Ohashi K
PLOS One, 18, 1, e0281143, Jan. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Spontaneous tumors are a major cause of death in cats. Treatment of human tumors has progressed dramatically in the past decade, partly due to the success of immunotherapies using immune checkpoint inhibitors, such as anti-programmed death 1 (PD-1) and anti-PD-ligand 1 (PD-L1) antibodies. However, little is known about the PD-1 pathway and its association with tumor disease in cats. This study investigated the applicability of anti-PD-1/PD-L1 therapy in feline tumors. We first determined the complete coding sequence of feline PD-L1 and PD-L2, and found that the deduced amino acid sequences of feline PD-L1/PD-L2 share high sequence identities (66-83%) with orthologs in other mammalian species. We prepared recombinant feline PD-1, PD-L1, and PD-L2 proteins and confirmed receptor-ligand binding between PD-1 and PD-L1/PD-L2 using flow cytometry. Next, we established an anti-feline PD-L1 monoclonal antibody (clone CL1Mab-7) to analyze the expression of PD-L1. Flow cytometry using CL1Mab-7 revealed the cell surface expression of PD-L1 in a feline macrophage (Fcwf-4) and five mammary adenocarcinoma cell lines (FKNp, FMCm, FYMp, FONp, and FONm), and showed that PD-L1 expression was upregulated by interferon-γ stimulation. Finally, immunohistochemistry using CL1Mab-7 also showed PD-L1 expression in feline squamous cell carcinoma (5/5, 100%), mammary adenocarcinoma (4/5, 80%), fibrosarcoma (5/5, 100%), and renal cell carcinoma (2/2, 100%) tissues. Our results strongly encourage further investigations of the PD-1/PD-L1 pathway as a potential therapeutic target for feline tumors.

Combined immune checkpoint blockade enhances antiviral immunity against bovine leukemia virus
Nakamura H, Konnai S, Okagawa T, Maekawa N, Sajiki Y, Watari K, Kamitani K, Saito M, Kato Y, Suzuki Y, Murata S, Ohashi K
J Virol., 97, 1, e0143022, Jan. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leukosis (EBL) in cattle and is widespread in many countries, including Japan. Recent studies have revealed that the expression of immunoinhibitory molecules, such as programmed death-1 (PD-1) and PD-ligand 1, plays a critical role in immunosuppression and disease progression during BLV infection. In addition, a preliminary study has suggested that another immunoinhibitory molecule, T-cell immunoglobulin domain and mucin domain-3 (TIM-3), is involved in immunosuppression during BLV infection. Therefore, this study was designed to further elucidate the immunoinhibitory role of immune checkpoint molecules in BLV infection. TIM-3 expression was upregulated on peripheral CD4+ and CD8+ T cells in BLV-infected cattle. Interestingly, in EBL cattle, CD4+ and CD8+ T cells infiltrating lymphomas expressed TIM-3. TIM-3 and PD-1 were upregulated and coexpressed in peripheral CD4+ and CD8+ T cells from BLV-infected cattle. Blockade by anti-bovine TIM-3 monoclonal antibody increased CD69 expression on T cells and gamma interferon (IFN-γ) production from peripheral blood mononuclear cells from BLV-infected cattle. A syncytium formation assay also demonstrated the antiviral effects of TIM-3 blockade against BLV infection. The combined inhibition of TIM-3 and PD-1 pathways significantly enhanced IFN-γ production and antiviral efficacy compared to inhibition alone. In conclusion, the combined blockade of TIM-3 and PD-1 pathways shows strong immune activation and antiviral effects and has potential as a novel therapeutic method for BLV infection. IMPORTANCE Enzootic bovine leukosis caused by bovine leukemia virus (BLV) is an important viral disease in cattle, causing severe economic losses to the cattle industry worldwide. The molecular mechanisms of BLV-host interactions are complex. Previously, it was found that immune checkpoint molecules, such as PD-1, suppress BLV-specific Th1 responses as the disease progresses. To date, most studies have focused only on how PD-1 facilitates escape from host immunity in BLV-infected cattle and the antiviral effects of the PD-1 blockade. In contrast, how T-cell immunoglobulin domain and mucin domain-3 (TIM-3), another immune checkpoint molecule, regulates anti-BLV immune responses is rarely reported. It is also unclear why PD-1 inhibition alone was insufficient to exert anti-BLV effects in previous clinical studies. In this study, the expression profile of TIM-3 in T cells derived from BLV-infected cattle suggested that TIM-3 upregulation is a cause of immunosuppression in infected cattle. Based on these results, anti-TIM-3 antibody was used to experimentally evaluate its function in influencing immunity against BLV. Results indicated that TIM-3 upregulation induced by BLV infection suppressed T-cell activation and antiviral cytokine production. Some T cells coexpressed PD-1 and TIM-3, indicating that simultaneous inhibition of PD-1 and TIM-3 with their respective antibodies synergistically restored antiviral immunity. This study could open new avenues for treating bovine chronic infections.

Diagnosis and early prediction of lymphoma using high-throughput clonality analysis of bovine leukemia virus-infected cells
Okagawa T, Shimakura H, Konnai S, Saito M, Matsudaira T, Nao N, Yamada S, Murakami K, Maekawa N, Murata S, Ohashi K
Microbiol Spectr., 10, 2, e0259522, American Society for Microbiology, Oct. 2022, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Bovine leukemia virus (BLV) infection causes aggressive B-cell lymphoma in cattle and sheep. The virus has spread to farms around the world, causing significant economic damage to the livestock industry.

乳酸菌Lactobacillus plantarum HOKKAIDO株による免疫制御機序の解明　　　　　　　　　　　　　　　
池端 麻里, 今内 覚, 岡川 朋弘, 阿部 健太郎, 本間 満, 北村 亨, 前川 直也, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [DI1A, 01], (公社)日本獣医学会, Sep. 2022
Japanese

ネコ腫瘍組織におけるprogrammed death ligand 1(PD-L1)の発現解析　　　　　　　　　　　　　　　
青木 絵理, 今内 覚, 前川 直也, 岡川 朋弘, 竹内 寛人, 佐藤 純平, 浅野 裕美恵, 大塚 拓海, 賀川 由美子, 加藤 幸成, 高木 哲, 鈴木 定彦, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [DI1A, 04], (公社)日本獣医学会, Sep. 2022
Japanese

鼻疽の診断抗原の探索　　　　　　　　　　　　　　　
市川 世識, 飯沼 由希帆, 岡川 朋弘, 前川 直也, 村田 史郎, 今内 覚, 木下 優太, 丹羽 秀和, 青島 圭佑, 小林 篤史, 大橋 和彦, 木村 享史
日本獣医学会学術集会講演要旨集, 165回, [DI1A, 09], (公社)日本獣医学会, Sep. 2022
Japanese

ダニ媒介性脳炎の治療法開発に向けた血液脳関門透過性分子の研究　　　　　　　　　　　　　　　
福田 美津紀, 深野 紗代, 小林 進太郎, 前川 直也, 高橋 侑嗣, 平野 港, 苅和 宏明, 今内 覚, 好井 健太朗
日本獣医学会学術集会講演要旨集, 165回, [F1P, 12], (公社)日本獣医学会, Sep. 2022
Japanese

広範な有効性を示すワクチン開発のための鳥類寄生ダニ由来システインプロテアーゼの特性解析(Characterization of cysteine proteases from avian mites to develop a vaccine with broad efficacy)　　　　　　　　　　　　　　　
Win Shwe Yee, 村田 史郎, 藤澤 宗太郎, 瀬尾 光里, 佐藤 純平, 佐藤 匠, 大石 英司, 種子野 章, 岡川 朋弘, 前川 直也, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [E3P, 02], (公社)日本獣医学会, Sep. 2022
English

ワクモ(Dermanyssus gallinae)由来シスタチン様分子の抗ワクモワクチン抗原としての評価　　　　　　　　　　　　　　　
藤澤 宗太郎, 村田 史郎, 伊勢崎 政美, 有泉 拓馬, 佐藤 匠, 大石 英司, 種子野 章, 前川 直也, 岡川 朋弘, 市居 修, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [E3P, 01], (公社)日本獣医学会, Sep. 2022
Japanese

ワクモ由来の2種類のアスパラギン酸ペプチダーゼの性状解析　　　　　　　　　　　　　　　
瀬尾 光里, 村田 史郎, Shwe Yee Win, 藤澤 宗太郎, 北條 巧, 伊勢崎 政美, 佐藤 匠, 大石 英司, 種子野 章, 市居 修, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [E3P, 03], (公社)日本獣医学会, Sep. 2022
Japanese

乳酸菌Lactobacillus plantarum HOKKAIDO株による免疫制御機序の解明　　　　　　　　　　　　　　　
池端 麻里, 今内 覚, 岡川 朋弘, 阿部 健太郎, 本間 満, 北村 亨, 前川 直也, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [DI1A, 01], (公社)日本獣医学会, Sep. 2022
Japanese

ネコ腫瘍組織におけるprogrammed death ligand 1(PD-L1)の発現解析　　　　　　　　　　　　　　　
青木 絵理, 今内 覚, 前川 直也, 岡川 朋弘, 竹内 寛人, 佐藤 純平, 浅野 裕美恵, 大塚 拓海, 賀川 由美子, 加藤 幸成, 高木 哲, 鈴木 定彦, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [DI1A, 04], (公社)日本獣医学会, Sep. 2022
Japanese

ダニ媒介性脳炎の治療法開発に向けた血液脳関門透過性分子の研究　　　　　　　　　　　　　　　
福田 美津紀, 深野 紗代, 小林 進太郎, 前川 直也, 高橋 侑嗣, 平野 港, 苅和 宏明, 今内 覚, 好井 健太朗
日本獣医学会学術集会講演要旨集, 165回, [F1P, 12], (公社)日本獣医学会, Sep. 2022
Japanese

ワクモ(Dermanyssus gallinae)由来シスタチン様分子の抗ワクモワクチン抗原としての評価　　　　　　　　　　　　　　　
藤澤 宗太郎, 村田 史郎, 伊勢崎 政美, 有泉 拓馬, 佐藤 匠, 大石 英司, 種子野 章, 前川 直也, 岡川 朋弘, 市居 修, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [E3P, 01], (公社)日本獣医学会, Sep. 2022
Japanese

広範な有効性を示すワクチン開発のための鳥類寄生ダニ由来システインプロテアーゼの特性解析(Characterization of cysteine proteases from avian mites to develop a vaccine with broad efficacy)　　　　　　　　　　　　　　　
Win Shwe Yee, 村田 史郎, 藤澤 宗太郎, 瀬尾 光里, 佐藤 純平, 佐藤 匠, 大石 英司, 種子野 章, 岡川 朋弘, 前川 直也, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [E3P, 02], (公社)日本獣医学会, Sep. 2022
English

ワクモ由来の2種類のアスパラギン酸ペプチダーゼの性状解析　　　　　　　　　　　　　　　
瀬尾 光里, 村田 史郎, Shwe Yee Win, 藤澤 宗太郎, 北條 巧, 伊勢崎 政美, 佐藤 匠, 大石 英司, 種子野 章, 市居 修, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 165回, [E3P, 03], (公社)日本獣医学会, Sep. 2022
Japanese

鼻疽の診断抗原の探索　　　　　　　　　　　　　　　
市川 世識, 飯沼 由希帆, 岡川 朋弘, 前川 直也, 村田 史郎, 今内 覚, 木下 優太, 丹羽 秀和, 青島 圭佑, 小林 篤史, 大橋 和彦, 木村 享史
日本獣医学会学術集会講演要旨集, 165回, [DI1A, 09], (公社)日本獣医学会, Sep. 2022
Japanese

Prostaglandin E2-induced immune suppression viacytotoxic T-lymphocyte antigen-4 in paratuberculosis
Sajiki Y, Konnai S, Watari K, Okagawa T, Tanaka A, Nagata R, Kawaji S, Maekawa N, Suzuki Y, Kato Y, Murata S, Mori Y, Ohashi K
Infect Immun., 90, 10, e0021022, Sep. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Paratuberculosis is a chronic enteritis of ruminants caused by the facultative intracellular pathogen Mycobacterium avium subsp. paratuberculosis. The Th1 response inhibits the proliferation of M. avium subsp. paratuberculosis during the early subclinical stage. However, we have previously shown that immune inhibitory molecules, such as prostaglandin E2 (PGE2), suppress M. avium subsp. paratuberculosis-specific Th1 responses as the disease progresses. To date, the mechanism underlying immunosuppression during M. avium subsp. paratuberculosis infection has not been elucidated. Therefore, in the present study, we investigated the function of cytotoxic T-lymphocyte antigen 4 (CTLA-4) expressed by peripheral blood mononuclear cells (PBMCs) from cattle with paratuberculosis because CTLA-4 expression is known to be elevated in T cells under an M. avium subsp. paratuberculosis experimental infection. M. avium subsp. paratuberculosis antigen induced CTLA-4 expression in T cells from cattle experimentally infected with M. avium subsp. paratuberculosis. Interestingly, both PGE2 and an E prostanoid 4 agonist also induced CTLA-4 expression in T cells. In addition, a functional assay with a bovine CTLA-4-immunogobulin fusion protein (CTLA-4-Ig) indicated that CTLA-4 inhibited gamma interferon (IFN-γ) production in M. avium subsp. paratuberculosis-stimulated PBMCs, while blockade by anti-bovine CTLA-4 monoclonal antibody increased the secretion of IFN-γ and tumor necrosis factor alpha production in these PBMCs. These preliminary findings show that PGE2 has immunosuppressive effects via CTLA-4 to M. avium subsp. paratuberculosis. Therefore, it is necessary to clarify in the future whether CTLA-4-mediated immunosuppression facilitates disease progression of paratuberculosis in cattle.

RAISING is a high-performance method for identifying random transgene integration sites
Wada Y, Sato T, Hasegawa H, Matsudaira T, Nao N, Coler-Reilly ALG, Tasaka T, Yamauchi S, Okagawa T, Momose H, Tanio M, Kuramitsu M, Sasaki D, Matsumoto N, Yagishita N, Yamauchi J, Araya N, Tanabe K, Yamagishi M, Nakashima M, Nakahata S, Iha H, Ogata M, Muramatsu M, Imaizumi Y, Uchimaru K, Miyazaki Y, Konnai S, Yanagihara K, Morishita K, Watanabe T, Yamano Y, Saito M
Commun Biol., 5, 1, 535, 535, Jun. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Both natural viral infections and therapeutic interventions using viral vectors pose significant risks of malignant transformation. Monitoring for clonal expansion of infected cells is important for detecting cancer. Here we developed a novel method of tracking clonality via the detection of transgene integration sites. RAISING (Rapid Amplification of Integration Sites without Interference by Genomic DNA contamination) is a sensitive, inexpensive alternative to established methods. Its compatibility with Sanger sequencing combined with our CLOVA (Clonality Value) software is critical for those without access to expensive high throughput sequencing. We analyzed samples from 688 individuals infected with the retrovirus HTLV-1, which causes adult T-cell leukemia/lymphoma (ATL) to model our method. We defined a clonality value identifying ATL patients with 100% sensitivity and 94.8% specificity, and our longitudinal analysis also demonstrates the usefulness of ATL risk assessment. Future studies will confirm the broad applicability of our technology, especially in the emerging gene therapy sector.

Exploration of serum biomarkers in dogs with malignant melanoma receiving anti-PD-L1 therapy and potential of COX-2 inhibition for combination therapy
Maekawa N, Konnai S, Asano Y, Sajiki Y, Deguchi T, Okagawa T, Watari K, Takeuchi H, Takagi S, Hosoya K, Kim S, Ohta H, Kato Y, Suzuki Y, Murata S, Ohashi K
Sci Rep., 12, 1, 9265, 9265, Jun. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Immune checkpoint inhibitors (ICIs) such as anti-PD-L1 antibodies are widely used to treat human cancers, and growing evidence suggests that ICIs are promising treatments for canine malignancies. However, only some canine oral malignant melanoma (OMM) cases respond to ICIs. To explore biomarkers predictive of survival in dogs with pulmonary metastatic OMM receiving the anti-PD-L1 antibody c4G12 (n = 27), serum concentrations of prostaglandin E2 (PGE2), cytokines, chemokines, and growth factors were measured prior to treatment initiation. Among 12 factors tested, PGE2, interleukin (IL)-12p40, IL-8, monocyte chemotactic protein-1 (MCP-1), and stem cell factor (SCF) were higher in OMM dogs compared to healthy dogs (n = 8). Further, lower baseline serum PGE2, MCP-1, and vascular endothelial growth factor (VEGF)-A concentrations as well as higher IL-2, IL-12, and SCF concentrations predicted prolonged overall survival. These observations suggest that PGE2 confers resistance against anti-PD-L1 therapy through immunosuppression and thus is a candidate target for combination therapy. Indeed, PGE2 suppressed IL-2 and interferon (IFN)-γ production by stimulated canine peripheral blood mononuclear cells (PBMCs), while inhibition of PGE2 biosynthesis using the COX-2 inhibitor meloxicam in combination with c4G12 enhanced Th1 cytokine production by PBMCs. Thus, serum PGE2 may be predictive of c4G12 treatment response, and concomitant use of COX-2 inhibitors may enhance ICI antitumor efficacy.

Suppressive effects of Ixodes persulcatus sialostatin L2 against Borrelia miyamotoi-stimulated immunity
Sajiki Y, Konnai S, Okagawa T, Maekawa N, Isezaki M, Yamada S, Ito T, Sato K, Kawabata H, Logullo C, da Silva Vaz I Jr, Murata S, Ohashi K
Ticks Tick Borne Dis., 13, 4, 101963, 101963, Apr. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Borrelia miyamotoi infection is an emerging tick-borne disease that causes hard tick-borne relapsing fever. B. miyamotoi is transmitted through the bite of ticks, including Ixodes persulcatus. Although accumulating evidence suggests that tick salivary proteins enhance the infectivity of other tick-borne pathogens, the association of B. miyamotoi with tick-derived proteins remains unknown. In this study, the effect of I. persulcatus sialostatin L2 (Ip-sL2), a tick-derived cystatin, on specific immunity to B. miyamotoi was preliminarily investigated in vitro. Mice were immunized with heat-killed B. miyamotoi and in vitro analyses of the splenocytes of the immunized mice indicated that the expression levels of the activation markers of CD11c+ and CD3+ cells were significantly upregulated by B. miyamotoi stimulation. Spleen cells from B. miyamotoi-immunized mice were used to determine whether Ip-sL2 regulates murine immune responses against B. miyamotoi. Treatment with Ip-sL2 in vitro inhibited the activation of CD11c+ and CD3+ cells as well as inflammatory cytokine production by cultured splenocytes. These findings show that Ip-sL2 has modulatory effects on murine immune responses to B. miyamotoi. Therefore, it is necessary to clarify in the future whether Ip-sL2 is involved in the enhanced infectivity of B. miyamotoi.

Estradiol-induced immune suppression via prostaglandin E2 during parturition in bovine leukemia virus-infected cattle
Sajiki Y, Konnai S, Okagawa T, Maekawa N, Goto S, Kohara J, Nitanai A, Takahashi H, Kubota K, Takeda H, Murata S, Ohashi K
PLOS One, 17, 3, e0263660, e0263660, Public Library of Science (PLoS), Mar. 2022, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Immune suppression during pregnancy and parturition is considered a risk factor that is related to the progression of bovine chronic diseases, such as bovine leukosis, which is caused by bovine leukemia virus (BLV). Our previous studies have demonstrated that prostaglandin E₂ (PGE₂) suppresses BLV-specific Th1 responses and contributes to the disease progression during BLV infection. Although PGE₂ reportedly plays important roles in the induction of parturition, PGE₂ involvement in immune suppression during parturition is unknown. To investigate its involvement, we analyzed PGE₂ kinetics and Th1 responses in BLV-infected pregnant cattle. PGE₂ concentrations in sera were increased, whereas IFN-γ responses were decreased before delivery. PGE₂ is known to suppress Th1 immune responses in cattle. Thus, these data suggest that PGE₂ upregulation inhibits Th1 responses during parturition. We also found that estradiol was important for PGE₂ induction in pregnant cattle. In vitro analyses indicated that estradiol suppressed IFN-γ production, at least in part, via PGE₂/EP4 signaling. In vivo analyses showed that estradiol administration significantly influenced the induction of PGE₂ production and impaired Th1 responses. Our data suggest that estradiol-induced PGE₂ is involved in the suppression of Th1 responses during pregnancy and parturition in cattle, which could contribute to the progression of BLV infection.

Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek’s Disease Virus on Transactivation Activity and Virulence　　　　　　　　　　　　　　　
Sato J, Murata S, Yang Z, Kaufer BB, Fujisawa F, Seo H, Maekawa N, Okagawa T, Konnai S, Osterrider N, Parcells MS, Ohashi K
Viruses, 14, 2, 382, Feb. 2022, [Peer-reviewed]
English, Scientific journal

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages.　　　　　　　　　　　　　　　
Gulay KCM, Aoshima K, Maekawa N, Suzuki T, Konnai S, Kobayashi A, Kimura T
Sci Rep., 12, 1, 2124, Feb. 2022, [Peer-reviewed]
English, Scientific journal

Redox imbalance induces remodeling of glucose metabolism in Rhipicephalus microplus embryonic cell line　　　　　　　　　　　　　　　
Della Noce B, Martins da Silva R, de Carvalho, Uhl MV, Konnai S, Ohashi K, Calixto C, Arcanjo A, de Abreu LA, de Carvalho SS, da Silva Vaz I Jr, Logullo C
J Biol Chem., 101599, Jan. 2022, [Peer-reviewed]
Scientific journal

Enhancement of interleukin-2 production by bovine peripheral blood mononuclear cells treated with the combination of anti-programmed death-ligand 1 and cytotoxic T lymphocyte antigen 4 chimeric monoclonal antibodies
Watari K, Konnai S, Okagawa T, Maekawa N, Sajiki Y, Kato Y, Suzuki Y, Murata S, Ohashi K
J Vet Med Sci., 84, 1, 6, 15, Jan. 2022, [Peer-reviewed], [Corresponding author], [Domestic magazines]
English, Scientific journal, Our previous studies demonstrate the therapeutic efficacy against bovine diseases of an anti-bovine programmed death-ligand 1 (PD-L1) chimeric antibody. In humans, PD-1 and PD-L1 antibodies are more effective when combined with an antibody targeting cytotoxic T lymphocyte antigen 4 (CTLA-4) and these combination therapies are therefore clinically used. Here we generated an anti-bovine CTLA-4 chimeric antibody (chAb) to enhance the therapeutic efficacy of the PD-L1 antibody. We further analyzed the effects of dual blockade of CTLA-4 and PD-1 pathways on T-cell responses. The established anti-bovine CTLA-4 chAb showed comparable blocking activity on the binding of bovine CTLA-4 to CD80 and CD86 as the anti-bovine CTLA-4 mouse monoclonal antibody. Anti-bovine CTLA-4 chAb also significantly increased IL-2 production from bovine peripheral blood mononuclear cells (PBMCs). Further, the combination of anti-CTLA-4 chAb with anti-PD-L1 chAb significantly upregulated IL-2 production by PBMCs. These results suggest that the combination of antibodies have higher potential to enhance immune responses against pathogens compared with single administration.

Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen
Fujisawa S, Murata S, Isezaki M, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K
Parasitology, 149, 1, 105, 115, Jan. 2022, [Peer-reviewed]
English, Scientific journal

In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens　　　　　　　　　　　　　　　
Ariizumi T, Murata S, Fujisawa S, Isezaki M, Sato T, Oishi E, Taneno A, Ichii O, Maekawa N, Okagawa T, Konnai S, Ohashi K
Poult Sci, 101, 3, 101638, Dec. 2021, [Peer-reviewed]
English, Scientific journal

Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens
Fujisawa S, Murata S, Isezaki M, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K
Vaccines (Basel), 9, 12, 1472, 1472, MDPI AG, Dec. 2021, [Peer-reviewed]
English, Scientific journal, Poultry red mite (PRM; Dermanyssus gallinae) is a hazardous, blood-sucking ectoparasite of birds that constitutes a threat to poultry farming worldwide. Acaricides, commonly used in poultry farms to prevent PRMs, are not effective because of the rapid emergence of acaricide-resistant PRMs. However, vaccination may be a promising strategy to control PRM. We identified a novel cystatin-like molecule in PRMs: Dg-Cys. Dg-Cys mRNA expression was detected in the midgut and ovaries, in all stages of life. The PRM nymphs that were artificially fed with the plasma from chickens that were immunized with Dg-Cys in vitro had a significantly reduced reproductive capacity and survival rate. Moreover, combination of Dg-Cys with other antigen candidates, like copper transporter 1 or adipocyte plasma membrane-associated protein, enhanced vaccine efficacies. vaccination and its application as an antigen for cocktail vaccines could be an effective strategy to reduce the damage caused by PRMs in poultry farming.

Programmed death-ligand 1 expression in swine chronic infections and enhancement of interleukin-2 production via programmed death-1/programmed death-ligand 1 blockade　　　　　　　　　　　　　　　
Ganbaatar O, Konnai S, Okagawa T, Nojima Y, Maekawa N, Ichikawa I, Kobayashi A, Shibahara T, Yanagawa Y, Higuchi H, Kato Y, Suzuki Y, Murata S, Ohashi K
Immun Inflamm Dis., 9, 4, 1573, 1583, Dec. 2021, [Peer-reviewed], [Corresponding author]
English

Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan　　　　　　　　　　　　　　　
Murata S, Yamamoto E, Sakashita N, Maekawa N, Okagawa T, Konnai S, Ohashi K
Poult Sci., 100, 11, 101461, Nov. 2021, [Peer-reviewed]
English, Scientific journal

In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens
Fujisawa S, Murata S, Takehara M, Aoyama J, Morita A, Isezaki M, Win SY, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K
Vaccine, 39, 41, 6057, 6066, Elsevier BV, Oct. 2021, [Peer-reviewed]
English, Scientific journal

ウシCTLA-4ならびにPD-L1を標的とした抗体併用法による免疫増強効果の検討　　　　　　　　　　　　　　　
渡 慧, 今内 覚, 岡川 朋弘, 前川 直也, 村田 史郎, 鈴木 定彦, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DIO, 4], (公社)日本獣医学会, Sep. 2021
Japanese

牛伝染性リンパ腫発症母牛における子宮内感染の解析　　　　　　　　　　　　　　　
嶋倉 穂南, 今内 覚, 岡川 朋弘, 中村 隼人, 神谷 可菜, 齋藤 麻矢, 前川 直也, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DVO, 5], (公社)日本獣医学会, Sep. 2021
Japanese

マレック病ウイルスMeqタンパク質における挿入および欠損配列の病原性への影響　　　　　　　　　　　　　　　
佐藤 純平, 村田 史郎, 藤澤 宗太郎, 瀬尾 光里, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DVO, 25], (公社)日本獣医学会, Sep. 2021
Japanese

イヌ腫瘍細胞株におけるProgrammed cell death-ligand 1の発現機序の解析　　　　　　　　　　　　　　　
大脇 稜, 出口 辰弥, 今内 覚, 前川 直也, 細谷 謙次, 金 尚昊, 須永 隆文, 奥村 正裕
日本獣医学会学術集会講演要旨集, 164回, [HSO, 19], (公社)日本獣医学会, Sep. 2021
Japanese

ワクモ(Dermanyssus gallinae)由来システインプロテアーゼの抗ワクモワクチン抗原としての評価　　　　　　　　　　　　　　　
有泉 拓馬, 村田 史郎, 藤澤 宗太郎, 伊勢崎 政美, 佐藤 匠, 大石 英司, 種子野 章, 市居 修, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [EO, 6], (公社)日本獣医学会, Sep. 2021
Japanese

鼻疽に対する診断用抗原の探索および血清診断法の確立　　　　　　　　　　　　　　　
飯沼 由希帆, 岡川 朋弘, 市川 世識, 陸 拾七, 前川 直也, 村田 史郎, 今内 覚, 木村 亨史, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DIO, 2], (公社)日本獣医学会, Sep. 2021
Japanese

Expression and functional analysis of swine PD-1/PD-L1 pathway(和訳中)　　　　　　　　　　　　　　　
Otgontuya Ganbaatar, 今内 覚, 岡川 朋弘, 野島 裕太郎, 前川 直也, 市川 世識, 小林 篤史, 芝原 友幸, 柳川 洋二郎, 鈴木 定彦, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DIO, 3], (公社)日本獣医学会, Sep. 2021
English

ウシCTLA-4ならびにPD-L1を標的とした抗体併用法による免疫増強効果の検討　　　　　　　　　　　　　　　
渡 慧, 今内 覚, 岡川 朋弘, 前川 直也, 村田 史郎, 鈴木 定彦, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DIO, 4], (公社)日本獣医学会, Sep. 2021
Japanese

牛伝染性リンパ腫発症母牛における子宮内感染の解析　　　　　　　　　　　　　　　
嶋倉 穂南, 今内 覚, 岡川 朋弘, 中村 隼人, 神谷 可菜, 齋藤 麻矢, 前川 直也, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DVO, 5], (公社)日本獣医学会, Sep. 2021
Japanese

マレック病ウイルスMeqタンパク質における挿入および欠損配列の病原性への影響　　　　　　　　　　　　　　　
佐藤 純平, 村田 史郎, 藤澤 宗太郎, 瀬尾 光里, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [DVO, 25], (公社)日本獣医学会, Sep. 2021
Japanese

ワクモ(Dermanyssus gallinae)由来システインプロテアーゼの抗ワクモワクチン抗原としての評価　　　　　　　　　　　　　　　
有泉 拓馬, 村田 史郎, 藤澤 宗太郎, 伊勢崎 政美, 佐藤 匠, 大石 英司, 種子野 章, 市居 修, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 164回, [EO, 6], (公社)日本獣医学会, Sep. 2021
Japanese

イヌ腫瘍細胞株におけるProgrammed cell death-ligand 1の発現機序の解析　　　　　　　　　　　　　　　
大脇 稜, 出口 辰弥, 今内 覚, 前川 直也, 細谷 謙次, 金 尚昊, 須永 隆文, 奥村 正裕
日本獣医学会学術集会講演要旨集, 164回, [HSO, 19], (公社)日本獣医学会, Sep. 2021
Japanese

Canine Transforming Growth Factor-β Receptor 2-Ig: A Potential Candidate Biologic for Melanoma Treatment That Reverses Transforming Growth Factor-β1 Immunosuppression
Hiroto Takeuchi, Satoru Konnai, Naoya Maekawa, Satoshi Takagi, Hiroshi Ohta, Noboru Sasaki, Sangho Kim, Tomohiro Okagawa, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi
Frontiers in Veterinary Science, 8, 656715, 14 Jun. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Molecular Epidemiology and Whole-Genome Analysis of Bovine Foamy Virus in Japan　　　　　　　　　　　　　　　
Mekata H, Okagawa T, Konnai S, Miyazawa T
Viruses, 13, 6, 1017, May 2021, [Peer-reviewed]
English

Differential expression of PEPCK isoforms is correlated to Aedes aegypti oogenesis and embryogenesis　　　　　　　　　　　　　　　
da Silva RM, Vital WO, Martins RS, Moraes J, Gomes H, Calixto C, Konnai S, Ohashi K, da Silva Vaz I Jr, Logullo C
Comp Biochem Physiol B Biochem Mol Biol., 256, 110618, May 2021, [Peer-reviewed]
English, Scientific journal

Prostaglandin-related immune suppression in cattle　　　　　　　　　　　　　　　
Sajiki Y, Konnai S, Ikenaka Y, Okagawa T, Maekawa N, Logullo C, da Silva Vaz Jr I, Murata S, Ohashi K
Vet Immunol Immunopathol, 236, 110238, Apr. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae)
Ariizumi T, Murata S, Fujisawa S, Isezaki M, Maekawa N, Okagawa T, Sato T, Oishi E, Taneno A, Konnai S, Ohashi K
J Vet Med Sci., 83, 4, 558, 565, Apr. 2021, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Poultry red mites (PRMs, Dermanyssus gallinae) are harmful ectoparasites that affect farmed chickens and cause serious economic losses in the poultry industry worldwide. Acaricides are used for PRM control; however, some PRMs have developed acaricide-resistant properties, which have indicated the need for different approaches for PRM control. Therefore, it is necessary to elucidate the biological status of PRMs to develop alternative PRM control strategies. Quantitative polymerase chain reaction (qPCR) allows analysis of the biological status at the transcript level. However, reference genes are preferable for accurate comparison of expression level changes given the large variation in the quality of the PRM samples collected in each farm. This study aimed to identify candidate reference genes with stable expression levels in the different blood feeding states and life stages of PRMs. First, we selected candidates based on the following criteria: sufficient expression intensity and no significant expression difference between fed and starved states. We selected and characterized seven candidate reference genes. Among them, we evaluated the gene expression stability between the starved and fed states using RefFinder; moreover, we compared their expression levels in each life-stage and identified two reference genes, Elongation factor 1-alpha (ELF1A)-like and apolipophorins-like. Finally, we evaluated the utility of the candidates as reference genes, and the use of ELF1A-like and apolipophorins-like successfully normalized ATP synthase subunit g -like gene expression. Thus, ELF1A-like and apolipophorins-like could be suitable reference genes in PRMs.

Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens.
Murata S, Taniguchi A, Isezaki M, Fujisawa S, Sakai E, Taneno A, Ichii O, Ito T, Maekawa N, Okagawa T, Konnai S, Ohashi K
Parasite, 28, 9, 9, 9, Feb. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Poultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different approaches are required to manage the problems caused by PRMs. Vaccination of chickens is one of the methods being considered to reduce the number of PRMs in poultry houses. In a previous study, a cysteine protease, Deg-CPR-1, was identified as a candidate vaccine against PRMs distributed in Europe. In this study, we investigated the characteristics of Deg-CPR-1. A phylogenetic analysis revealed that Deg-CPR-1 is closely related to the digestive cysteine proteases of other mite species, and it was classified into a cluster different from that of chicken cathepsins. Deg-CPR-1 of PRMs in Japan has an amino acid substitution compared with that of PRMs in Europe, but it showed efficacy as a vaccine, consistent with previous findings. Deg-CPR-1 exhibited cathepsin L-like enzyme activity. In addition, the Deg-CPR-1 mRNA was expressed in the midgut and in all stages of PRMs that feed on blood. These results imply that Deg-CPR-1 in the midgut may have important functions in physiological processes, and the inhibition of its expression may contribute to the efficacy of a Deg-CPR-1-based vaccine. Further research is required to fully understand the mechanisms of vaccine efficacy.

PD-L1 immunohistochemistry for canine cancers and clinical benefit of anti-PD-L1 antibody in dogs with pulmonary metastatic oral malignant melanoma
Maekawa N, Konnai S, Nishimura M, Kagawa Y, Takagi S, Hosoya K, Ohta H, Kim S, Okagawa T, Izumi Y, Deguchi T, Kato Y, Yamamoto S, Yamamoto K, Toda M, Nakajima C, Suzuki Y, Murata S, Ohashi K
NPJ Precis Oncol., 5, 1, 10, 10, Feb. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Immunotherapy targeting programmed cell death 1 (PD-1) and PD-ligand 1 (PD-L1) represents promising treatments for human cancers. Our previous studies demonstrated PD-L1 overexpression in some canine cancers, and suggested the therapeutic potential of a canine chimeric anti-PD-L1 monoclonal antibody (c4G12). However, such evidence is scarce, limiting the clinical application in dogs. In the present report, canine PD-L1 expression was assessed in various cancer types, using a new anti-PD-L1 mAb, 6C11-3A11, and the safety and efficacy of c4G12 were explored in 29 dogs with pulmonary metastatic oral malignant melanoma (OMM). PD-L1 expression was detected in most canine malignant cancers including OMM, and survival was significantly longer in the c4G12 treatment group (median 143 days) when compared to a historical control group (n = 15, median 54 days). In dogs with measurable disease (n = 13), one dog (7.7%) experienced a complete response. Treatment-related adverse events of any grade were observed in 15 dogs (51.7%). Here we show that PD-L1 is a promising target for cancer immunotherapy in dogs, and dogs could be a useful large animal model for human cancer research.

The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosis
Sajiki Y, Konnai S, Nagata R, Kawaji S, Nakamura H, Fujisawa S, Okagawa T, Maekawa N, Kato Y, Suzuki Y, Murata S, Mori Y, Ohashi K
J Vet Med Sci., 83, 2, 162, 166, Feb. 2021, [Peer-reviewed], [Corresponding author], [Domestic magazines]
English, Scientific journal, Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne's disease.

Cross-species reactivity of antibodies against Ixodes persulcatus ferritin 2 to Rhipicephalus microplus
Xavier AM, Konnai S, Parizi FP, Githaka WN, Isezaki M, Goto S, Fujisawa S, Yamada S, Okagawa T, Maekawa N, Logullo C, Silva Vaz J r I, Murata S, Ohashi K
Jpn J Vet Res., 69, 1, 57, 65, Feb. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Intrauterine infection with Mycobacterium avium subsp. paratuberculosis in pregnant cattle diagnosed with Johne’s disease
Taniguchi Y, Konnai S, Sakakibara S, Yamamoto A, Okagawa T, Maekawa N, Murata S, Ohashi K
Jpn J Vet Res, 69, 1, 51, 55, Feb. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

The Role of Saliva in Arthropod-Host-Pathogen Relationships　　　　　　　　　　　　　　　
Tirloni L, Calvo E, Konnai S, Silva Vaz Jr I
Front Cell Infect Microbiol, 10, 630626, Jan. 2021, [Peer-reviewed], [Invited]
English, Scientific journal

Direct evidence of the preventive effect of milk replacer–based probiotic feeding in calves against severe diarrhea
Kayasaki F, Okagawa T, Konnai S, Kohara J, Sajiki Y, Watari K, Ganbaatar O, Goto S, Nakamura H, Shimakura H, Minato E, Kobayashi A, Kubota M, Terasaki N, Takeda A, Noda H, Honma M, Maekawa N, Murata S, Ohashi K
Vet Microbiol., 254, 108976., 108976, Elsevier BV, Jan. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
Sajiki Y, Konnai S, Ikenaka Y, Gulay K, Kobayashi A, Parizi L, João B, Watari K, Fujisawa S, Okagawa T, Maekawa N, Logullo C, Silva Vaz Jr I, Murata S, Ohashi K
Sci Rep., 11, 1, 1063, Springer Science and Business Media LLC, Jan. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal, The tick Rhipicephalus microplus is a harmful parasite of cattle that causes considerable economic losses to the cattle breeding industry. Although R. microplus saliva (Rm-saliva) contains several immunosuppressants, any association between Rm-saliva and the expression of immunoinhibitory molecules, such as programmed death (PD)-1 and PD-ligand 1 (PD-L1), has not been described. In this study, flow cytometric analyses revealed that Rm-saliva upregulated PD-1 expression in T cells and PD-L1 expression in CD14⁺ and CD11c⁺ cells in cattle. Additionally, Rm-saliva decreased CD69 expression in T cells and Th1 cytokine production from peripheral blood mononuclear cells. Furthermore, PD-L1 blockade increased IFN-γ production in the presence of Rm-saliva, suggesting that Rm-saliva suppresses Th1 responses via the PD-1/PD-L1 pathway. To reveal the upregulation mechanism of PD-1/PD-L1 by Rm-saliva, we analyzed the function of prostaglandin E₂ (PGE₂), which is known as an inducer of PD-L1 expression, in Rm-saliva. We found that Rm-saliva contained a high concentration of PGE₂, and PGE₂ treatment induced PD-L1 expression in CD14⁺ cells in vitro. Immunohistochemical analyses revealed that PGE₂ and PD-L1 expression was upregulated in tick-attached skin in cattle. These data suggest that PGE₂ in Rm-saliva has the potential to induce the expression of immunoinhibitory molecules in host immune cells.

A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus
Sajiki Y, Konnai S, Okagawa T, Maekawa N, Nakamura H, Kato Y, Suzuki Y, Murata S, Ohashi K
Dev Comp Immunol., 114, 103847, 103847, Elsevier BV, Jan. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Enhanced immunotherapeutic efficacy of anti-PD-L1 antibody in combination with an EP4 antagonist
Sajiki Y, Konnai S, Cai Z, Takada K, Okagawa T, Maekawa N, Fujisawa S, Kato Y, Suzuki Y, Murata S, Ohashi K
ImmunoHorizons, 4, 12, 837, 850, The American Association of Immunologists, Dec. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal

The suppression of Th1 response by inducing TGF-β1 from regulatory T cells in bovine mycoplasmosis
Sajiki Y, Konnai S, Goto S, Okagawa T, Ohira K, Shimakura H, Maekawa N, Gondaira S, Higuchi H, Tajima M, Hirano Y, Kohara J, Murata S, Ohashi K
Front Vet Sci., 7, 609443, Frontiers Media SA, Dec. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Regulatory T cells (Tregs) regulate immune responses and maintain host immune homeostasis. Tregs contribute to the disease progression of several chronic infections by oversuppressing immune responses via the secretion of immunosuppressive cytokines, such as transforming growth factor (TGF)-β and interleukin-10. In the present study, we examined the association of Tregs with Mycoplasma bovis infection, in which immunosuppression is frequently observed. Compared with uninfected cattle, the percentage of Tregs, CD4⁺CD25^highFoxp3⁺ T cells, was increased in M. bovis-infected cattle. Additionally, the plasma of M. bovis-infected cattle contained the high concentrations of TGF-β1, and M. bovis infection induced TGF-β1 production from bovine immune cells in in vitro cultures. Finally, we analyzed the immunosuppressive effects of TGF-β1 on bovine immune cells. Treatment with TGF-β1 significantly decreased the expression of CD69, an activation marker, in T cells, and Th1 cytokine production in vitro. These results suggest that the increase in Tregs and TGF-β1 secretion could be one of the immunosuppressive mechanisms and that lead to increased susceptibility to other infections in terms of exacerbation of disease during M. bovis infection.

Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar.
Yang Z, Murata S, Fujisawa S, Takehara M, Katakura K, Hmoon MM, Win SY, Bawm S, Konnai S, Ohashi K
BMC Vet Res., 16, 1, 453, Nov. 2020, [Peer-reviewed]
English, Scientific journal

Genetic characterization of a Marek's disease virus strain isolated in Japan.　　　　　　　　　　　　　　　
Murata S, Machida Y, Isezaki M, Maekawa N, Okagawa T, Konnai S, Ohashi K
Virol J., 17, 1, 186, Nov. 2020, [Peer-reviewed]
English, Scientific journal

PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade
Ganbaatar O, Konnai S, Okagawa T, Nojima Y, Maekawa N, Minato E, Kobayashi A, Ando R, Sasaki N, Miyakoshi D, Ichii O, Kato Y, Suzuki Y, Murata S, Ohashi K
PLOS ONE, 15, 11, e0234218, Nov. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Programmed death-1 (PD-1) is an immunoinhibitory receptor expressed on lymphocytes. Interaction of PD-1 with its ligand PD-ligand 1 (PD-L1) delivers inhibitory signals and impairs proliferation, cytokine production, and cytotoxicity of T cells. In our previous studies, we have developed anti-bovine PD-L1 monoclonal antibodies (mAbs) and reported that the PD-1/PD-L1 pathway was closely associated with T-cell exhaustion and disease progression in bovine chronic infections and canine tumors. Furthermore, we found that blocking antibodies that target PD-1 and PD-L1 restore T-cell functions and could be used in immunotherapy in cattle and dogs. However, the immunological role of the PD-1/PD-L1 pathway for chronic equine diseases, including tumors, remains unclear. In this study, we identified cDNA sequences of equine PD-1 (EqPD-1) and PD-L1 (EqPD-L1) and investigated the role of anti-bovine PD-L1 mAbs against EqPD-L1 using in vitro assays. In addition, we evaluated the expression of PD-L1 in tumor tissues of equine malignant melanoma (EMM). The amino acid sequences of EqPD-1 and EqPD-L1 share a considerable identity and similarity with homologs from non-primate species. Two clones of the anti-bovine PD-L1 mAbs recognized EqPD-L1 in flow cytometry, and one of these cross-reactive mAbs blocked the binding of equine PD-1/PD-L1. Of note, immunohistochemistry confirmed the PD-L1 expression in EMM tumor tissues. A cultivation assay revealed that PD-L1 blockade enhanced the production of Th1 cytokines in equine immune cells. These findings showed that our anti-PD-L1 mAbs would be useful for analyzing the equine PD-1/PD-L1 pathway. Further research is warranted to discover the immunological role of PD-1/PD-L1 in chronic equine diseases and elucidate a future application in immunotherapy for horses.

Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze
Githaka N, Konnai S, Isezaki M, Goto S, Xavier M, Fujisawa S, Yamada S, Okagawa T, Maekawa N, Logullo C, da Silva Vaz Jr I, Murata S, Ohashi K
Ticks Tick-Borne Dis, 11, 6, 101547, 101547, Elsevier BV, Nov. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal

牛白血病ウイルス感染症における免疫抑制受容体TIM-3の発現解析および機能解析　　　　　　　　　　　　　　　
中村 隼人, 今内 覚, 岡川 朋弘, 佐治木 大和, 渡 慧, 神谷 可菜, 齋藤 麻矢, 前川 直也, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 163回, 221, 221, (公社)日本獣医学会, Oct. 2020
Japanese

ネコCytotoxic T-lymphocyte antigen-4(CTLA-4)を標的とした新規免疫抑制剤の開発における基礎的検討　　　　　　　　　　　　　　　
大塚 拓海, 今内 覚, 前川 直也, 渡 慧, 岡川 朋弘, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 163回, 221, 221, (公社)日本獣医学会, Oct. 2020
Japanese

ワクモにおける定量的PCR法確立のための内在性コントロール遺伝子の探索　　　　　　　　　　　　　　　
有泉 拓馬, 村田 史郎, 藤澤 宗太郎, 伊勢崎 政美, 前川 直也, 岡川 朋弘, 種子野 章, 大石 英司, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 163回, 248, 248, (公社)日本獣医学会, Oct. 2020
Japanese

Development and Characterization of Anti-Sheep Podoplanin Monoclonal Antibodies PMab-253 and PMab-260.
Mika K Kaneko, Masato Sano, Junko Takei, Teizo Asano, Yusuke Sayama, Hideki Hosono, Atsushi Kobayashi, Satoru Konnai, Yukinari Kato
Monoclonal antibodies in immunodiagnosis and immunotherapy, 39, 4, 144, 155, 16 Jul. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Anti-podoplanin (PDPN) monoclonal antibodies (mAbs) are needed as markers for lymphatic endothelial cells or type I alveolar cells in immunohistochemical analyses. We have developed anti-PDPN mAbs for many species, including humans, mice, rats, rabbits, dogs, cats, bovines, pigs, Tasmanian devils, alpacas, tigers, whales, goats, horses, and bears. This study develops and characterizes anti-sheep PDPN (sPDPN) mAbs using Cell-Based Immunization and Screening (CBIS) method. A RAP14 tag was added to the N-terminus of sPDPN, and an anti-RAP14 tag mAb (PMab-2) was used to measure the expression level of sPDPN in flow cytometry and Western blots. We immunized mice with sPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/sPDPN) cells and screened mAbs against sPDPN using flow cytometry. Two of the mAbs, PMab-253 (immunoglobulin M [IgM], kappa) and PMab-260 (IgM, kappa), detected CHO/sPDPN cells specifically using flow cytometry and Western blots. Both PMab-253 and PMab-260 stained the renal glomerulus and Bowman's capsule, lymphatic endothelial cells of the lung and colon, and type I alveolar cells of the lung, suggesting PMab-253 and PMab-260, which were developed by CBIS method, can be applied to functional analyses of sPDPN. We also determined the binding epitope of PMab-253 and PMab-260 using flow cytometry. Analysis of sPDPN deletion mutants revealed that the N-terminus of the PMab-253 and PMab-260 epitope exists between amino acids 110 and 115 of sPDPN. Analysis of sPDPN point mutations revealed that the critical epitope of PMab-253 and PMab-260 includes Thr112 and Ser113 of sPDPN, indicating that the PMab-253 and PMab-260 epitope are independent of the platelet aggregation-stimulating (PLAG) domain or the PLAG-like domain of sPDPN.

Transcriptome dynamics of blood-fed and starved poultry red mites, Dermanyssus gallinae　　　　　　　　　　　　　　　
Fujisawa S, Murata S, Isezaki M, Oishi E, Taneno A, Maekawa N, Okagawa T, Konnai S, Ohashi K
Parasitol Int., 102156, 102156, Jun. 2020, [Peer-reviewed]
English, Scientific journal

Expression Analysis of Canine CMTM6 and CMTM4 as Potential Regulators of the PD-L1 Protein in Canine Cancers
Takeuchi H, Konnai S, Maekawa N, Minato E, Ichikawa Y, Kobayashi A, Okegawa T, Murata S, Ohashi K.
Front Vet Sci, 7, 330, 330, Jun. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Cancer is one of the most significant causes of death in dogs. Antibody drugs targeting the PD-1/PD-L1 axis represent a promising immunotherapy for both human and canine cancers. However, the regulation mechanisms of PD-L1 expression in canine cancers require further investigation to better understand the resistance mechanisms to anti-PD-L1 therapy. Recent reports have shown that CMTM6 and CMTM4 are critical regulators of PD-L1 protein expression in human cancer cells. By preventing PD-L1 from lysosome-mediated degradation, CMTM6 maintains PD-L1 expression on the cell surface. However, the literature has not reported on CMTM6 and CMTM4 in dogs, and their functions are completely unknown. To reveal a regulation mechanism of PD-L1 in canine cancers, this study firstly identified the gene sequences of CMTM6 and CMTM4. Then, the expression analysis of these proteins was performed by immunohistochemistry. Furthermore, the functions of CMTM6 and CMTM4 in regulating PD-L1 expression were examined by gene knockdown of CMTM6 and CMTM4. Canine CMTM6 and CMTM4 displayed high amino acid sequence identities compared with those of humans and mice. An immunohistochemical analysis using cross-reactive antibodies revealed that canine malignant melanoma and osteosarcoma express CMTM6, CMTM4, and PD-L1 simultaneously. Gene knockdown of CMTM6 and CMTM4 with RNA interference significantly reduced the cell surface expression of PD-L1 in a canine cell line. These results suggest that CMTM6 and CMTM4 are regulators of PD-L1 expression in canine cancers and could serve as potential therapeutic targets to enhance antitumor immunity.

Rhipicephalus microplus cystatin as a potential cross-protective tick vaccine against Rhipicephalus appendiculatus　　　　　　　　　　　　　　　
Parizi L, Rangel C, Sabadina G, Saggin B, Kiio I, Xavier M, Matos R, Camargo-Mathias M, Seixas A, Konnai S, Ohashi K, Githaka N, da Silva Vaz Jr I
Ticks Tick-Borne Dis, 11, 3, 101378, May 2020, [Peer-reviewed]
English, Scientific journal

Clinical efficacy of the combined treatment of anti-PD-L1 rat-bovine chimeric antibody with a COX-2 inhibitor in calves infected with Mycoplasma bovis
Shinya Goto, Satoru Konnai, Yuki Hirano, Junko Kohara, Tomohiro Okagawa, Naoya Maekawa, Yamato Sajiki, Kei Watari, Erina Minato, Atsuhi Kobayashi, Satoshi Gondaira, Hidetoshi Higuchi, Masateru Koiwa, Motoshi Tajima, Eiji Taguchi, Masaru Ishida, Ryoko Uemura, Shinji Yamada, Mika K. Kaneko, Yukinari Kato, Keiichi Yamamoto, Mikihiro Toda, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi
JAPANESE JOURNAL OF VETERINARY RESEARCH, 68, 2, 77, 90, May 2020, [Peer-reviewed]
English, Scientific journal

Immunosuppressive effects of sialostatin L1 and L2 isolated from the taiga tick Ixodes persulcatus Schulze
Yamato Sajiki, Satoru Konnai, Akie Ochi, Tomohiro Okagawa, Naftaly Githaka, Masayoshi Isezaki, Shinji Yamada, Takuya Ito, Shuji Ando, Hiroki Kawabata, Carlos Logullo, Itabajara da Silva Vaz, Naoya Maekawa, Shiro Murata, Kazuhiko Ohashi
Ticks and Tick-borne Diseases, 11, 2, 101332, 101332, Elsevier BV, Mar. 2020, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
Satoshi Gondaira, Koji Nishi, Takahiro Tanaka, Takashi Yamamoto, Takanori Nebu, Reina Watanabe, Satoru Konnai, Tomohito Hayashi, Yoshio Kiku, Mariko Okamoto, Kazuya Matsuda, Masateru Koiwa, Hidetomo Iwano, Hajime Nagahata, Hidetoshi Higuchi
Infection and immunity, 88, 3, e00521, 19, 20 Feb. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Mycoplasma bovis is a destructive pathogen that causes large economic losses in rearing cattle for beef and dairy worldwide. M. bovis causes suppression of and evades the host immune response; however, the mechanisms of host immune function involved in M. bovis mastitis have not been elucidated. The purpose of this study was to elucidate the characteristics of the bovine immune response to mycoplasmal mastitis. We evaluated the responsiveness of the bovine mammary gland following infusion of M. bovis Somatic cell counts and bacterial counts in milk from the infected quarter were increased. However, the proliferation of peripheral blood mononuclear cells (blood MNCs) and mononuclear cells isolated from M. bovis-stimulated mammary lymph nodes (lymph node MNCs) did not differ from that in the unstimulated cells. Transcriptome analysis revealed that the mRNA levels of innate immune system-related genes in blood MNCs, complement factor D (CFD), ficolin 1 (FCN1), and tumor necrosis factor superfamily member 13 (TNFSF13) decreased following intramammary infusion of M. bovis The mRNA levels of immune exhaustion-related genes, programmed cell death 1 (PD-1), programmed cell death-ligand 1 (PD-L1), lymphocyte activation gene 3 (LAG3), and cytotoxic T-lymphocyte-associated protein 4 (CTLA4) of milk mononuclear cells (milk MNCs) in the infected quarter were increased compared with those before infusion. Increase in immune exhaustion-related gene expression and decrease in innate immune response-related genes of MNCs in quarters from cows were newly characterized by M. bovis-induced mastitis. These results suggested that M. bovis-induced mastitis affected the immune function of bovine MNCs, which is associated with prolonged duration of infection with M. bovis.

Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection.
Shinya Goto, Satoru Konnai, Yuki Hirano, Junko Kohara, Tomohiro Okagawa, Naoya Maekawa, Yamato Sajiki, Kei Watari, Erina Minato, Atsuhi Kobayashi, Satoshi Gondaira, Hidetoshi Higuchi, Masateru Koiwa, Motoshi Tajima, Eiji Taguchi, Ryoko Uemura, Shinji Yamada, Mika K Kaneko, Yukinari Kato, Keiichi Yamamoto, Mikihiro Toda, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi
Frontiers in veterinary science, 7, 12, 12, 12, Feb. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bovine mycoplasmosis caused by Mycoplasma bovis results in pneumonia and mastitis in cattle. We previously demonstrated that the programmed death 1 (PD-1)/PD-ligand 1 (PD-L1) pathway is involved in immune dysfunction during M. bovis infection and that prostaglandin E2 (PGE2) suppressed immune responses and upregulated PD-L1 expression in Johne's disease, a bacterial infection in cattle. In this study, we investigated the role of PGE2 in immune dysfunction and the relationship between PGE2 and the PD-1/PD-L1 pathway in M. bovis infection. In vitro stimulation with M. bovis upregulated the expressions of PGE2 and PD-L1 presumably via Toll-like receptor 2 in bovine peripheral blood mononuclear cells (PBMCs). PGE2 levels of peripheral blood in infected cattle were significantly increased compared with those in uninfected cattle. Remarkably, plasma PGE2 levels were positively correlated with the proportions of PD-L1+ monocytes in M. bovis-infected cattle. Additionally, plasma PGE2 production in infected cattle was negatively correlated with M. bovis-specific interferon (IFN)-γ production from PBMCs. These results suggest that PGE2 could be one of the inducers of PD-L1 expression and could be involved in immunosuppression during M. bovis infection. In vitro blockade assays using anti-bovine PD-L1 antibody and a cyclooxygenase 2 inhibitor significantly upregulated the M. bovis-specific IFN-γ response. Our study findings might contribute to the development of novel therapeutic strategies for bovine mycoplasmosis that target PGE2 and the PD-1/PD-L1 pathway.

PMab-241 Specifically Detects Bear Podoplanin of Lymphatic Endothelial Cells in the Lung of Brown Bear.
Junko Takei, Shinji Yamada, Satoru Konnai, Tsuyoshi Ishinazaka, Michito Shimozuru, Mika K Kaneko, Yukinari Kato
Monoclonal antibodies in immunodiagnosis and immunotherapy, 38, 6, 282, 284, Dec. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Podoplanin (PDPN)/T1alpha is utilized as a specific marker of lymphatic endothelial cells or type I alveolar cells of lung. Therefore, sensitive and specific monoclonal antibodies (mAbs) detecting PDPN are necessary for immunohistochemical analyses, especially using formalin-fixed paraffin-embedded tissues. Recently, we developed an anti-bear PDPN (bPDPN) mAb, PMab-247, which is useful for immunohistochemical analyses to detect both lymphatic endothelial cells and type I alveolar cells of lung. However, it is difficult to distinguish lymphatic endothelial cells from type I alveolar cells in the bear lung. In this study, we showed that a novel anti-bPDPN mAb, PMab-241 stained only lymphatic endothelial cells, not type I alveolar cells of the lung in immunohistochemical analyses. These findings suggest that PMab-241 could be useful for staining lymphatic endothelial cells specifically in the bear lung tissues.

Epitope Mapping of Anti-Bear Podoplanin Monoclonal Antibody PMab-247　　　　　　　　　　　　　　　
Kato Y, Takei J, Furusawa Y, Sayama Y, Sano M, Konnai S, Kobayashi A, Harada H, Takahashi M, Suzuki H, Yamada S, Kaneko MK
Monoclon Antib Immunodiagn Immunother., 38, 5, 230, 233, Sep. 2019, [Peer-reviewed]
English, Scientific journal

Immune inhibitory function of bovine CTLA-4 and the effects of its blockade in IFN-γ production　　　　　　　　　　　　　　　
Watari K, Konnai S, Maekawa N, Okagawa T, Suzuki Y, Murata S, Ohashi K
BMC Vet Res, 15, 1, 380, Sep. 2019, [Peer-reviewed]
English, Scientific journal

ミャンマーの養鶏場におけるマイコプラズマ、鶏伝染性気管支炎ウイルス、鶏伝染性喉頭気管炎ウイルスの分子学的検出と遺伝学的性状解析(Molecular detection and genetic characterization of mycoplasmas, infectious bronchitis virus and infectious laryngotracheitis virus in poultry farms in Myanmar)　　　　　　　　　　　　　　　
Yang Zhiyuan, 藤澤 宗太郎, 村田 史郎, 竹原 昌生, 片倉 賢, Myint Myint Hmoon, Shwe Yee Win, Saw Bawm, Lat Lat Htun, Ye Htut Aung, Mar Mar Win, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 162回, 411, 411, (公社)日本獣医学会, Aug. 2019
English

イヌ腫瘍組織およびイヌ腫瘍由来細胞株におけるイヌHER2の発現解析　　　　　　　　　　　　　　　
吉武 志江奈, 今内 覚, 前川 直也, 賀川 由美子, 西村 麻紀, 岡川 朋弘, 鈴木 定彦, 高木 哲, 中川 貴之, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 162回, 380, 380, (公社)日本獣医学会, Aug. 2019
Japanese

イヌ腫瘍由来細胞株におけるCKLF-like MARVEL transmembrane domain containing protein 6(CMTM6)および4(CMTM4)の発現解析　　　　　　　　　　　　　　　
竹内 寛人, 今内 覚, 岡川 朋弘, 前川 直也, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 162回, 380, 380, (公社)日本獣医学会, Aug. 2019
Japanese

BLV感染症に対するCOX-2阻害剤と抗PD-L1抗体併用法の抗ウイルス効果の検討　　　　　　　　　　　　　　　
佐治木 大和, 今内 覚, 岡川 朋弘, 前川 直也, 後藤 伸也, 小原 潤子, 山田 慎二, 加藤 幸成, 鈴木 定彦, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 162回, 381, 381, (公社)日本獣医学会, Aug. 2019
Japanese

Mycoplasma bovis感染症に対する抗PD-L1キメラ抗体を用いた臨床試験　　　　　　　　　　　　　　　
後藤 伸也, 今内 覚, 平野 佑気, 小原 潤子, 岡川 朋弘, 前川 直也, 鈴木 定彦, 加藤 幸成, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 162回, 381, 381, (公社)日本獣医学会, Aug. 2019
Japanese

RNA-Seqを用いたワクモ(Dermanyssus gallinae)の吸血状態別における遺伝子発現解析　　　　　　　　　　　　　　　
藤澤 宗太郎, 村田 史郎, 竹原 昌生, 伊勢崎 政美, 小川 遼, 宇野 有紀子, 種子野 章, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
日本獣医学会学術集会講演要旨集, 162回, 412, 412, (公社)日本獣医学会, Aug. 2019
Japanese

COX-2阻害剤の併用による抗PD-L1抗体を用いたイヌ腫瘍免疫療法の効果増強に向けた基礎的検討　　　　　　　　　　　　　　　
前川 直也, 今内 覚, 浅野 裕美恵, 岡川 朋弘, 高木 哲, 村田 史郎, 大橋 和彦
日本獣医学会学術集会講演要旨集, 162回, 460, 460, (公社)日本獣医学会, Aug. 2019
Japanese

ネコのアクロメガリー関連インスリン抵抗性糖尿病のコントロールを目的とした成長ホルモン受容体/Fc融合タンパク質の作製　　　　　　　　　　　　　　　
鈴木 理紗, 和泉 雄介, 前川 直也, 今内 覚, 高木 哲, 森下 啓太郎, 細谷 謙次, 滝口 満喜
日本獣医学会学術集会講演要旨集, 162回, 469, 469, (公社)日本獣医学会, Aug. 2019
Japanese

PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis
Takei J, Furusawa Y, Yamada S, Nakamura T, Sayama Y, Sano M, Konnai S, Kobayashi A, Harada H, Kaneko MK, Kato Y
Monoclon Antib Immunodiagn Immunother., 38, 4, 171, 174, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Podoplanin (PDPN) is utilized as a specific marker of type I alveolar cells of lung and lymphatic endothelial cells of every tissue. Therefore, sensitive and specific monoclonal antibodies detecting PDPN are necessary for immunohistochemical analyses, especially using formalin-fixed paraffin-embedded tissues. Recently, we developed an anti-bear PDPN (bPDPN) mAb, PMab-247, which is useful for Western blot, flow cytometry, and immunohistochemical analyses. In this study, immunohistochemical analyses showed that PMab-247 strongly detected bPDPN, which is expressed in type I alveolar cells and lymphatic endothelial cells of bear lung and podocytes of bear kidney. These findings suggest that PMab-247 could be useful for pathophysiological analyses using immunohistochemistry.

Prostaglandin E2 induced immune exhaustion and enhancement of anti-viral effects by anti-PD-L1 antibody combined with COX-2 inhibitor in bovine leukemia virus infection
Sajiki Y, Konnai S, Okagawa T, Nishimori A, Maekawa N, Goto S, Watari K, Minato E, Kobayashi A, Kohara J, Yamada S, Kaneko M, Kato Y, Takahashi H, Terasaki N, Takeda A, Yamamoto K, Toda M, Suzuki Y, Murata S, Ohashi K
J. Immunol, 203, 5, 1313, 1324, Aug. 2019, [Peer-reviewed]
English, Scientific journal

Vector transmission of bovine leukemia virus during summer season in Northern Hokkaido
Inagaki H, Konnai S, Kaburagi H, Murota H, Takabatake N, Watari K, Okagawa T, Maekawa N, Murata S, Ohashi K
Jpn. J. Vet. Res, 67, 3, 235, 239, Aug. 2019, [Peer-reviewed]
English, Scientific journal

Immunohistochemical Analysis of the Harbor Porpoise Using Antipodoplanin Antibody PMab-237.
Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Junko Takei, Masato Sano, Satoru Konnai, Atsushi Kobayashi, Shotaro Nakagun, Yoshiyasu Kobayashi, Mika K Kaneko, Yukinari Kato
Monoclonal antibodies in immunodiagnosis and immunotherapy, 38, 3, 104, 107, Jun. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Podoplanin (PDPN)/T1 alpha is known as a specific marker of lymphatic endothelial cells and type I alveolar cells. Sensitive and specific monoclonal antibodies (mAbs) for PDPN are needed for immunohistochemical analyses. Recently, we developed an anticetacean PDPN mAb, PMab-237. Herein, immunohistochemical analyses showed that PMab-237 strongly detected pulmonary type I alveolar cells, renal podocytes, and lymphatic endothelial cells of the harbor porpoise. These findings suggest that PMab-237 may be useful for immunohistochemical analyses for cetacean tissues.

Haematophagous mites on poultry farms in the Republic of the Union of Myanmar
Takehara M, Murata S, Katakura K, Fujisawa S, Hmoon MM, Win SY, Bawn S, Htun LL, Aung YH, Win MM, Isezaki M, Maekawa N, Okagawa T, Konnai S, Ohashi K
Heliyon, 5, 4, e01544, Apr. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Haematophagous ectoparasites of poultry, such as Ornithonyssus sylviarum, northern fowl mites (NFMs), Dermanyssus gallinae, poultry red mites (PRMs), and Ornithonyssus bursa, tropical fowl mites (TFMs) are prevalent worldwide. Although poultry farming is a major industry in Southeast Asia, there are only a few reports concerning the prevalence of avian mites in this region. In this study, we sampled twenty farms in four major poultry farming areas in Myanmar. We detected the mites on six farms, and they showed morphological similarities to NFMs and TFMs. The nucleotide sequences of cytochrome c oxidase subunit I indicated that some mites were NFMs. This is the first report confirming the presence of NFMs and TFMs among the hematophagous mites infesting chickens on Myanmar poultry farms.

Carbohydrate Metabolic Compensation Coupled to High Tolerance to Oxidative Stress in Ticks　　　　　　　　　　　　　　　
Noce BD, Uhl M, Machado J, Waltero C, Abreu L, Silva R, Fonseca R, Barros C, Sabadin G, Konnai S, Silva Vaz Jr. I, Ohashi K, Logullo C
Scientific Rep., 9, 4753, Mar. 2019, [Peer-reviewed]
English, Scientific journal

Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: Potential for bovine inflammation therapy
Fujisawa S, Konnai S, Okagawa T, Maekawa N, Tanaka A, Suzuki Y, Murata S, Ohashi K
BMC Vet Res., 15, 1, 68, 68, Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Refractory diseases, including bacterial infections, are causing huge economic losses in dairy farming. Despite efforts to prevent and treat those diseases in cattle, including the use of antimicrobials, it is not well controlled in the field. Several inflammatory cytokines, including tumor necrosis factor alpha (TNF-α), play important roles in disease progression; thus, blocking these cytokines can attenuate the acute and sever inflammation and may be a novel strategy for treatment. However, biological drugs targeting inflammatory cytokines have not been used in cattle. Therefore, in this study, bovine sTNFR1 and sTNFR2 IgG1 Fc-fusion proteins (TNFR1-Ig and TNFR2-Ig) were produced, and their anti-inflammatory functions were analyzed in vitro, to develop decoy receptors for bovine TNF-α. RESULTS: Both TNFR1-Ig and TNFR2-Ig were shown to bind with TNF-α, and TNFR2-Ig showed higher affinity toward TNF-α than TNFR1-Ig. We next stimulated murine fibroblast-derived cells (L929 cells) with TNF-α to induce cell death and analyzed cell viability in the presence of TNFR-Ig proteins. Both TNFR1-Ig and TNFR2-Ig suppressed TNF-α-induced cell death, significantly improving cell viability. In addition, cell death induced by TNF-α was suppressed, even at low TNFR2-Ig concentrations, suggesting TNFR2-Ig has higher activity to suppress TNF-α functions than TNFR1-Ig. Finally, to examine TNFR2-Ig's anti-inflammatory, we cultured peripheral blood mononuclear cells from cattle with TNF-α in the presence of TNFR2-Ig and analyzed the gene expression and protein production of the inflammatory cytokines IL-1β and TNF-α. TNFR2-Ig significantly reduced the gene expression and protein production of these cytokines. Our results suggest that TNFR2-Ig inhibits inflammatory cytokine kinetics by blocking TNF-α to transmembrane TNFR, thereby attenuating excessive inflammation induced by TNF-α. CONCLUSIONS: Collectively, the findings of this study demonstrated the potential of TNFR2-Ig as a novel therapeutic for inflammatory diseases, such as bovine clinical mastitis. Further investigation is required for future clinical application.

Anti-Horse Podoplanin Monoclonal Antibody PMab-219 is Useful for Detecting Lymphatic Endothelial Cells by Immunohistochemical Analysis　　　　　　　　　　　　　　　
Kato Y, Yamada S, Itai S, Kobayashi A, Konnai S, Kaneko MK
Monoclon Antib Immunodiagn Immunother., 37, 6, 272, 274, Dec. 2018, [Peer-reviewed]
English, Scientific journal

Detection of Alpaca Podoplanin by Immunohistochemistry Using the Antibovine Podoplanin Monoclonal Antibody PMab-44　　　　　　　　　　　　　　　
Kato Y, Yamada S, Itai S, Konnai S, Kobayashi A, Kaneko MK
Monoclon Antib Immunodiagn Immunother., 37, 6, 269, 271, Dec. 2018, [Peer-reviewed]
English, Scientific journal

Immunohistochemical Detection of Sheep Podoplanin Using an Antibovine Podoplanin Monoclonal Antibody PMab-44　　　　　　　　　　　　　　　
Kato Y, Yamada S, Itai S, Kobayashi A, Konnai S, Kaneko MK
Monoclon Antib Immunodiagn Immunother., 37, 6, 265, 268, Dec. 2018, [Peer-reviewed]
English, Scientific journal

The mouse-canine chimeric anti-dog podoplanin antibody P38B exerts antitumor activity in mouse xenograft models　　　　　　　　　　　　　　　
Kato Y, Ohishi T, Kawada M, Maekawa N, Konnai S, Itai S, Yamada S, Kaneko MK
Biochem Biophys Rep., 17, 23, 26, Nov. 2018, [Peer-reviewed]
English, Scientific journal

Identification of immuno-inhibitory molecules in Mongolian native cattle and yak
Ochirkhuu N, Konnai S, Odbileg R, Okagawa T, Maekawa N, Murata S, Ohashi K
Jpn J Vet Res, 66, 3, 177, 192, Aug. 2018, [Peer-reviewed]
English, Scientific journal

Cattle with a low bovine leukemia virus proviral load are rarely an infectious source　　　　　　　　　　　　　　　
Mekata H, Yamamoto M, Hayashi T, Kirino Y, Sekiguchi S, Konnai S, Horii Y, Norimine J
Jpn J Vet Res, 66, 3, 157, 163, Aug. 2018, [Peer-reviewed]
English, Scientific journal

Cooperation of PD-1 and LAG-3 in the exhaustion of CD4+ and CD8+ T cells during bovine leukemia virus infection.
Tomohiro Okagawa, Satoru Konnai, Asami Nishimori, Naoya Maekawa, Shinya Goto, Ryoyo Ikebuchi, Junko Kohara, Yasuhiko Suzuki, Shinji Yamada, Yukinari Kato, Shiro Murata, Kazuhiko Ohashi
Veterinary research, 49, 1, 50, 50, 19 Jun. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bovine leukemia virus (BLV) is a retrovirus that infects B cells in cattle and causes bovine leukosis after a long latent period. Progressive exhaustion of T cell functions is considered to facilitate disease progression of BLV infection. Programmed death-1 (PD-1) and lymphocyte activation gene-3 (LAG-3) are immunoinhibitory receptors that contribute to T-cell exhaustion caused by BLV infection in cattle. However, it is unclear whether the cooperation of PD-1 and LAG-3 accelerates disease progression of BLV infection. In this study, multi-color flow cytometric analyses of PD-1- and LAG-3-expressing T cells were performed in BLV-infected cattle at different stages of the disease. The frequencies of PD-1+LAG-3+ heavily exhausted T cells among CD4+ and CD8+ T cells was higher in the blood of cattle with B-cell lymphoma over that of BLV-uninfected and BLV-infected cattle without lymphoma. In addition, blockade assays of peripheral blood mononuclear cells were performed to examine whether inhibition of the interactions between PD-1 and LAG-3 and their ligands by blocking antibodies could restore T-cell function during BLV infection. Single or dual blockade of the PD-1 and LAG-3 pathways reactivated the production of Th1 cytokines, interferon-γ and tumor necrosis factor-α, from BLV-specific T cells of the infected cattle. Taken together, these results indicate that PD-1 and LAG-3 cooperatively mediate the functional exhaustion of CD4+ and CD8+ T cells and are associated with the development of B-cell lymphoma in BLV-infected cattle.

Epitope mapping of anti-mouse podoplanin monoclonal antibody PMab-1　　　　　　　　　　　　　　　
Yamada S, Itai S, Kaneko MK, Konnai S, Kato Y
Biochem Biophys Rep., 15, 52, 56, Jun. 2018, [Peer-reviewed]
English, Scientific journal

Evaluation of costimulatory molecules in dogs with B cell high grade lymphoma　　　　　　　　　　　　　　　
Tagawa M, Kurashima C, Takagi S, Maekawa N, Konnai S, Shimbo G, Matsumoto K, Inokuma H, Kawamoto K, Miyahara K
PLoS One, 13, 7, e0201222, Jun. 2018, [Peer-reviewed]
English, Scientific journal

Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease　　　　　　　　　　　　　　　
Sajiki Y, Konnai S, Okagawa T, Nishimori A, Maekawa N, Goto S, Ikebuchi R, Nagata R, Kawaji S, Kagawa Y, Yamada S, Kato Y, Nakajima C, Suzuki Y, Murata S, Mori Y, Ohashi K
Infect Immun., 86, 5, e00910-17, Apr. 2018, [Peer-reviewed]
English, Scientific journal

Identification of an Atypical Enzootic Bovine Leukosis in Japan by Using a Novel Classification of Bovine Leukemia Based on Immunophenotypic Analysis　　　　　　　　　　　　　　　
Nishimori A, Konnai S, Okagawa T, Maekawa N, Goto S, Ikebuchi R, Nakahara A, Chiba Y, Ikeda M, Murata S, Ohashi K
Clin Vaccine Immunol., 24, 12, e00001-18, Apr. 2018, [Peer-reviewed]
English, Scientific journal

New hematological key for bovine leukemia virus-infected Japanese Black cattle.
Hirohisa Mekata, Mari Yamamoto, Yumi Kirino, Satoshi Sekiguchi, Satoru Konnai, Yoichiro Horii, Junzo Norimine
The Journal of veterinary medical science, 80, 2, 316, 319, 20 Feb. 2018, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The European Community's (EC) Key, which is also called Bendixen's Key, is a well-established bovine leukemia virus (BLV) diagnostic method that classifies cattle according to the absolute lymphocyte count and age. The EC Key was originally designed for dairy cattle and is not necessarily suitable for Japanese Black (JB) beef cattle. This study revealed the lymphocyte counts in the BLV-free and -infected JB cattle were significantly lower than those in the Holstein cattle. Therefore, applying the EC Key to JB cattle could result in a large number of undetected BLV-infected cattle. Our proposed hematological key, which was designed for JB cattle, improves the detection of BLV-infected cattle by approximately 20%. We believe that this study could help promote BLV control.

Molecular detection and phylogenetic analysis of Ehrlichia canis in a Philippine dog
Maekawa N, Konnai S, Balbin MM, Mingala CN, Gicana KRB, Bernando FAEM, Murata S, Ohashi K
Ticks Tick Borne Dis., 9, 2, 266, 269, Feb. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Canine monocytic ehrlichiosis (CME), caused by a rickettsial bacterium, Ehrlichia canis, is distributed worldwide, particularly in tropical and subtropical regions. Transmission of E. canis is primarily mediated by the vector tick, Rhipicephalus sanguineus sensu lato and the bacteria then infect and replicate in monocytes and macrophages. Many cases are seen in veterinary hospitals and treated routinely; however, the genetic variation of E. canis strains found in the Philippines has been poorly investigated to date. In this study, the 16S rRNA gene and the gp200 gene of E. canis were detected by polymerase chain reaction from an infected dog in the Philippines, and the deduced amino acid sequence of the gp200 gene was subjected to a phylogenetic analysis. The Philippine genotype formed a cluster with the Taiwan genotype, and was somewhat divergent from the USA and Brazil strains. This suggested that E. canis underwent evolution in East and Southeast Asia, confirming the utility of the gp200 gene for the assessment of genetic relationships among strains.

Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis
Adriana Seixas, Maria Fernanda Alzugaray, Lucas Tirloni, Luis Fernando Parizi, Antonio Frederico Michel Pinto, Naftaly Wang'ombe Githaka, Satoru Konnai, Kazuhiko Ohashi, John R. Yates, Carlos Termignoni, Itabajara da Silva Vaz
TICKS AND TICK-BORNE DISEASES, 9, 1, 72, 81, Jan. 2018, [Peer-reviewed]
English, Scientific journal

Intrauterine infection with bovine leukemia virus in pregnant dam with high viral load.
Yamato Sajiki, Satoru Konnai, Asami Nishimori, Tomohiro Okagawa, Naoya Maekawa, Shinya Goto, Masashi Nagano, Junko Kohara, Nana Kitano, Toshihiko Takahashi, Motoshi Tajima, Hirohisa Mekata, Yoichiro Horii, Shiro Murata, Kazuhiko Ohashi
The Journal of veterinary medical science, 79, 12, 2036, 2039, 22 Dec. 2017, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Enzootic bovine leukemia is caused by the bovine leukemia virus (BLV). BLV is transmitted vertically or horizontally through the transfer of infected cells via direct contact, through milk, insect bites and contaminated iatrogenic procedures. However, we lacked direct evidence of intrauterine infection. The purpose of this study was to confirm intrauterine BLV infection in two pregnant dams with high viral load by cesarean delivery. BLV was detected in cord and placental blood, and the BLV in the newborns showed 100% nucleotide identity with the BLV-env sequence from the dams. Notably, a newborn was seropositive for BLV but had no colostral antibodies. In this study, we presented a direct evidence of intrauterine BLV transmission in pregnant dam with a high proviral load. These results could aid the development of BLV control measures targeting viral load.

Expression of Cat Podoplanin in Feline Squamous Cell Carcinomas
Shunsuke Itai, Shinji Yamada, Mika K. Kaneko, Hiroyuki Harada, Yumiko Kagawa, Satoru Konnai, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 36, 6, 243, 250, Mary Ann Liebert Inc., 01 Dec. 2017, [Peer-reviewed]
English, Scientific journal

Detection and molecular characterization of equine infectious anemia virus in Mongolian horses
Tumenjargal Sharav, Satoru Konnai, Nyamsuren Ochirkhuu, Erdene T. S. Ochir, Hirohisa Mekata, Yoshihiro Sakoda, Takashi Umemura, Shiro Murata, Tungalag Chultemdorj, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 79, 11, 1884, 1888, Nov. 2017, [Peer-reviewed]
English, Scientific journal

PMab-52: Specific and Sensitive Monoclonal Antibody Against Cat Podoplanin for Immunohistochemistry
Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Noriko Saidoh, Yao-Wen Chang, Saori Handa, Hiroyuki Harada, Yumiko Kagawa, Osamu Ichii, Satoru Konnai, Mika K. Kaneko, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 36, 5, 224, 230, Mary Ann Liebert Inc., 01 Oct. 2017, [Peer-reviewed]
English, Scientific journal

Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis
Goto S, Konnai S, Okagawa T, Nishimori A, Maekawa N, Gondaira S, Higuchi H, Koiwa M, Tajima M, Kohara J, Ogasawara S, Kato Y, Suzuki Y, Murata S, Ohashi K
Immun Inflamm Dis., 5, 3, 355, 363, Sep. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal, INTRODUCTION: Bovine mycoplasma, chiefly Mycoplasma bovis, is a pathogen that causes pneumonia, mastitis, arthritis, and otitis media in cattle. This pathogen exerts immunosuppressive effects, such as the inhibition of interferon production. However, the mechanisms involved in bovine mycoplasmosis have not been fully elucidated. In this study, we investigated the role of the programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) pathway in immunosuppression in bovine mycoplasmosis. METHODS: In the initial experiments, we used enzyme-linked immunosorbent assay to measure interferon-γ (IFN-γ) from peripheral blood mononuclear cells (PBMCs) isolated from cattle with mycoplasmosis. RESULTS: Expectedly, IFN-γ production significantly decreased in cattle with mycoplasmosis compared with that in clinically healthy cattle. Concomitantly, flow cytometric analysis revealed that the proportions of PD-1+ CD4+ and PD-L1+ CD14+ cells significantly increased in peripheral blood of the infected cattle. Interestingly, the number of PD-1+ CD4+ and PD-1+ CD8+ T cells were negatively correlated with IFN-γ production from PBMCs in bovine mycoplasmosis. Additionally, blockade of the PD-1/PD-L1 pathway in vitro by anti-bovine PD-1- and anti-bovine PD-L1 antibodies significantly upregulated the production of IFN-γ from anti-mycoplasma-specific cells. CONCLUSIONS: These results suggest that the PD-1/PD-L1 pathway could be involved in immune exhaustion of bovine mycoplasma-specific T cells. In conclusion, our study opens up a new perspective in the therapeutic strategy for bovine mycoplasmosis by targeting the immunoinhibitory receptor pathways.

Molecular Epidemiological Survey and Genetic Characterization of Anaplasma Species in Mongolian Livestock
Nyamsuren Ochirkhuu, Satoru Konnai, Raadan Odbileg, Shiro Murata, Kazuhiko Ohashi
VECTOR-BORNE AND ZOONOTIC DISEASES, 17, 8, 539, 549, Aug. 2017, [Peer-reviewed]
English, Scientific journal

A canine chimeric monoclonal antibody targeting PD-L1 and its clinical efficacy in canine oral malignant melanoma or undifferentiated sarcoma
Maekawa N, Konnai S, Takagi S, Kagawa Y, Okagawa T, Nishimori A, Ikebuchi R, Izumi Y, Deguchi T, Nakajima C, Kato Y, Yamamoto K, Uemura H, Suzuki Y, Murata S, Ohashi K
Sci Rep., 7, 1, 8951, 8951, Aug. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal, Immunotherapy targeting immune checkpoint molecules, programmed cell death 1 (PD-1) and PD-ligand 1 (PD-L1), using therapeutic antibodies has been widely used for some human malignancies in the last 5 years. A costimulatory receptor, PD-1, is expressed on T cells and suppresses effector functions when it binds to its ligand, PD-L1. Aberrant PD-L1 expression is reported in various human cancers and is considered an immune escape mechanism. Antibodies blocking the PD-1/PD-L1 axis induce antitumour responses in patients with malignant melanoma and other cancers. In dogs, no such clinical studies have been performed to date because of the lack of therapeutic antibodies that can be used in dogs. In this study, the immunomodulatory effects of c4G12, a canine-chimerised anti-PD-L1 monoclonal antibody, were evaluated in vitro, demonstrating significantly enhanced cytokine production and proliferation of dog peripheral blood mononuclear cells. A pilot clinical study was performed on seven dogs with oral malignant melanoma (OMM) and two with undifferentiated sarcoma. Objective antitumour responses were observed in one dog with OMM (14.3%, 1/7) and one with undifferentiated sarcoma (50.0%, 1/2) when c4G12 was given at 2 or 5 mg/kg, every 2 weeks. c4G12 could be a safe and effective treatment option for canine cancers.

Characterization of the Anti-Bovine Podoplanin Monoclonal Antibody PMab-44
Shinji Yamada, Ryusuke Honma, Mika K. Kaneko, Takuro Nakamura, Miyuki Yanaka, Noriko Saidoh, Michiaki Takagi, Satoru Konnai, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 36, 3, 129, 134, Mary Ann Liebert Inc., 01 Jun. 2017, [Peer-reviewed]
English, Scientific journal

Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle
Tomohiro Okagawa, Satoru Konnai, Asami Nishimori, Naoya Maekawa, Ryoyo Ikebuchi, Shinya Goto, Chie Nakajima, Junko Kohara, Satoshi Ogasawara, Yukinari Kato, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi
FRONTIERS IN IMMUNOLOGY, 8, 650, Jun. 2017, [Peer-reviewed]
English, Scientific journal

Molecular characterization of the lymphocyte activation gene-3 (LAG-3, CD223) of swamp- and riverine-type water buffaloes (Bubalus bubalis)
Shanemae M. Rivera, Ryan Bismark C. Padiernos, Evaristo A. Abella, Satoru Konnai, Claro N. Mingala
JAPANESE JOURNAL OF VETERINARY RESEARCH, 65, 2, 65, 74, May 2017, [Peer-reviewed]
English, Scientific journal

Clinical and subclinical bovine leukemia virus infection in a dairy cattle herd in Zambia
Girja S. Pandey, Edgar Simulundu, Danstan Mwiinga, Kenny L. Samui, Aaron S. Mweene, Masahiro Kajihara, Alfred Mangani, Racheal Mwenda, Joseph Ndebe, Satoru Konnai, Ayato Takada
ARCHIVES OF VIROLOGY, 162, 4, 1051, 1056, Apr. 2017, [Peer-reviewed]
English, Scientific journal

Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin
Shinji Yamada, Mika K. Kaneko, Takuro Nakamura, Osamu Ichii, Satoru Konnai, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 36, 2, 77, 79, Mary Ann Liebert Inc., 01 Apr. 2017, [Peer-reviewed]
English, Scientific journal

In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) ratbovine chimeric antibody against bovine leukemia virus infection
Asami Nishimori, Satoru Konnai, Tomohiro Okagawa, Naoya Maekawa, Ryoyo Ikebuchi, Shinya Goto, Yamato Sajiki, Yasuhiko Suzuki, Junko Kohara, Satoshi Ogasawara, Yukinari Kato, Shiro Murata, Kazuhiko Ohashi
PLOS ONE, 12, 4, e0174916, Apr. 2017, [Peer-reviewed]
English, Scientific journal

Isolation and purification of Gallid herpesvirus 2 strains currently distributed in Japan
Yuka Machida, Shiro Murata, Ayumi Matsuyama-Kato, Masayoshi Isezaki, Akira Taneno, Eishi Sakai, Satoru Konnai, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 79, 1, 115, 122, Jan. 2017, [Peer-reviewed]
English, Scientific journal

Immune exhaustion during chronic infections in cattle
Satoru Konnai, Shiro Murata, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 79, 1, 1, 5, Jan. 2017, [Peer-reviewed], [Invited]
English, Scientific journal

A novel mechanism of functional cooperativity regulation by thiol redox status in a dimeric inorganic pyrophosphatase
Evenilton P. Costa, Arnoldo R. Facanha, Criscila S. Cruz, Jhenifer N. Silva, Josias A. Machado, Gabriel M. Carvalho, Mariana R. Fernandes, Renato Martins, Eldo Campos, Nelilma C. Romeiro, Naftaly W. Githaka, Satoru Konnai, Kazuhiko Ohashi, Itabajara S. Vaz, Carlos Logullo
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1861, 1, 2922, 2933, Jan. 2017, [Peer-reviewed]
English, Scientific journal

Molecular and structural characterization of novel cystatins from the taiga tick Ixodes persulcatus
Carolina K. Rangel, Luis F. Parizi, Gabriela A. Sabadin, Evenilton P. Costa, Nelilma C. Romeiro, Masayoshi Isezaki, Naftaly W. Githaka, Adriana Seixas, Carlos Logullo, Satoru Konnai, Kazuhiko Ohashi, Itabajara da Silva Vaz
TICKS AND TICK-BORNE DISEASES, 8, 3, 432, 441, 2017, [Peer-reviewed]
English, Scientific journal

Molecular epidemiological survey and genetic characterization of ovine gammaherpesvirus-2 in Mongolian livestock
Nyamsuren Ochirkhuu, Satoru Konnai, Raadan Odbileg, Shiro Murata, Kazuhiko Ohashi
Journal of Veterinary Medical Science, 79, 12, 2040, 2042, Japanese Society of Veterinary Science, 2017, [Peer-reviewed]
English, Scientific journal

Podoplanin Expression in Canine Melanoma
Satoshi Ogasawara, Ryusuke Honma, Mika K. Kaneko, Yuki Fujii, Yumiko Kagawa, Satoru Konnai, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 35, 6, 304, 306, Mary Ann Liebert Inc., 01 Dec. 2016, [Peer-reviewed]
English, Scientific journal

Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle
Tomohiro Okagawa, Satoru Konnai, James R. Deringer, Massaro W. Ueti, Glen A. Scoles, Shiro Murata, Kazuhiko Ohashi, Wendy C. Brown
INFECTION AND IMMUNITY, 84, 10, 2779, 2790, Oct. 2016, [Peer-reviewed]
English, Scientific journal

PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas
Mika K. Kaneko, Ryusuke Honma, Satoshi Ogasawara, Yuki Fujii, Takuro Nakamura, Noriko Saidoh, Michiaki Takagi, Yumiko Kagawa, Satoru Konnai, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 35, 5, 263, 266, Mary Ann Liebert Inc., 01 Oct. 2016, [Peer-reviewed]
English, Scientific journal

Molecular detection and characterization of bovine viral diarrhea virus in Mongolian cattle and yaks
Nyamsuren Ochirkhuu, Satoru Konnai, Raadan Odbileg, Battogtokh Odzaya, Shura Gansukh, Shiro Murata, Kazuhiko Ohashi
ARCHIVES OF VIROLOGY, 161, 8, 2279, 2283, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Specific Detection of Dog Podoplanin Expressed in Renal Glomerulus by a Novel Monoclonal Antibody PMab-38 in Immunohistochemistry
Ryusuke Honma, Mika K. Kaneko, Satoshi Ogasawara, Yuki Fujii, Satoru Konnai, Michiaki Takagi, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 35, 4, 212, 216, Mary Ann Liebert Inc., 01 Aug. 2016, [Peer-reviewed]
English, Scientific journal

PMab-44 Detects Bovine Podoplanin in Immunohistochemistry　　　　　　　　　　　　　　　
Honma R, Ogasawara S, Kaneko MK, Fujii Y, Oki H, Nakamura T, Takagi M, Konnai S, Kato Y
Monoclon Antib Immunodiagn Immunother., Jun. 2016, [Peer-reviewed]
English, Scientific journal

Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma
Naoya Maekawa, Satoru Konnai, Tomohiro Okagawa, Asami Nishimori, Ryoyo Ikebuchi, Yusuke Izumi, Satoshi Takagi, Yumiko Kagawa, Chie Nakajima, Yasuhiko Suzuki, Yukinari Kato, Shiro Murata, Kazuhiko Ohashi
PLOS ONE, 11, 6, e0157176, Jun. 2016, [Peer-reviewed]
English, Scientific journal

Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection
Asami Nishimori, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Ayako Nakahara, Shiro Murata, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 78, 5, 791, 796, May 2016, [Peer-reviewed]
English, Scientific journal

Detection of bovine leukemia virus and identification of its genotype in Mongolian cattle
Nyamsuren Ochirkhuu, Satoru Konnai, Raadan Odbileg, Asami Nishimori, Tomohiro Okagawa, Shiro Murata, Kazuhiko Ohashi
ARCHIVES OF VIROLOGY, 161, 4, 985, 991, Apr. 2016, [Peer-reviewed]
English, Scientific journal

Critical Epitope of Anti-Rabbit Podoplanin Monoclonal Antibodies for Immunohistochemical Analysis
Ryusuke Honma, Yuki Fujii, Satoshi Ogasawara, Hiroharu Oki, Satoru Konnai, Yumiko Kagawa, Michiaki Takagi, Mika K. Kaneko, Yukinari Kato
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 35, 2, 65, 72, Mary Ann Liebert Inc., 01 Apr. 2016, [Peer-reviewed]
English, Scientific journal

Evaluation of Costimulatory Molecules in Peripheral Blood Lymphocytes of Canine Patients with Histiocytic Sarcoma
Michihito Tagawa, Naoya Maekawa, Satoru Konnai, Satoshi Takagi
PLOS ONE, 11, 2, e0150030, Feb. 2016, [Peer-reviewed]
English, Scientific journal

Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze
Kochi Toyomane, Satoru Konnai, Ayano Niwa, Naftaly Githaka, Masayoshi Isezaki, Shinji Yamada, Takuya Ito, Ai Takano, Shuji Ando, Hiroki Kawabata, Shiro Murata, Kazuhiko Ohashi
TICKS AND TICK-BORNE DISEASES, 7, 1, 119, 125, 2016, [Peer-reviewed]
English, Scientific journal

Bovine leukemia virus reduces anti-viral cytokine activities and NK cytotoxicity by inducing TGF-β secretion from regulatory T cells
Ohira K, Nakahara A, Konnai S, Okagawa T, Nishimori A, Maekawa N, Ikebuchi R, Kohara J, Murata S, Ohashi K
Immun Inflamm Dis., 4, 1, 52, 63, Jan. 2016, [Peer-reviewed], [International Magazine]
English, Scientific journal, CD4(+)CD25(high)Foxp3(+) T cells suppress excess immune responses that lead to autoimmune and/or inflammatory diseases, and maintain host immune homeostasis. However, CD4(+)CD25(high)Foxp3(+) T cells reportedly contribute to disease progression by over suppressing immune responses in some chronic infections. In this study, kinetic and functional analyses of CD4(+)CD25(high)Foxp3(+) T cells were performed in cattle with bovine leukemia virus (BLV) infections, which have reported immunosuppressive characteristics. In initial experiments, production of the Th1 cytokines IFN-γ and TNF-α was reduced in BLV-infected cattle compared with uninfected cattle, and numbers of IFN-γ or TNF-α producing CD4(+) T cells decreased with disease progression. In contrast, IFN-γ production by NK cells was inversely correlated with BLV proviral loads in infected cattle. Additionally, during persistent lymphocytosis disease stages, NK cytotoxicity was depressed as indicated by low expression of the cytolytic protein perforin. Concomitantly, total CD4(+)CD25(high)Foxp3(+) T cell numbers and percentages of TGF-β(+) cells were increased, suggesting that TGF-β plays a role in the functional declines of CD4(+) T cells and NK cells. In further experiments, recombinant bovine TGF-β suppressed IFN-γ and TNF-α production by CD4(+) T cells and NK cytotoxicity in cultured cells. These data suggest that TGF-β from CD4(+)CD25(high)Foxp3(+) T cells is immunosuppressive and contributes to disease progression and the development of opportunistic infections during BLV infection.

Molecular characterization of T-cell immunoglobulin mucin domain-3 and Galectin-9 genes of swamp- and riverine-type water buffaloes
P. L. H. Duran, R. B. C. Padiernos, E. A. Abella, S. Konnai, C. N. Mingala
INTERNATIONAL JOURNAL OF IMMUNOGENETICS, 42, 6, 469, 478, Dec. 2015, [Peer-reviewed]
English, Scientific journal

A preliminary survey of the seroprevalence of Mycobacterium avium subspecies paratuberculosis in Mongolian cattle
Nyamsuren Ochirkhuu, Satoru Konnai, Raadan Odbileg, Shiro Murata, Kazuhiko Ohashi
JAPANESE JOURNAL OF VETERINARY RESEARCH, 63, 4, 191, 194, Nov. 2015, [Peer-reviewed]
English, Scientific journal

Metal extent in blood of livestock from Dandora dumping site, Kenya: Source identification of Pb exposure by stable isotope analysis
Hokuto Nakata, Shouta M. M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Chihiro Ishii, Yared B. Yohannes, Satoru Konnai, Wageh Sobhy Darwish, Mayumi Ishizuka
ENVIRONMENTAL POLLUTION, 205, 8, 15, Oct. 2015, [Peer-reviewed]
English, Scientific journal

Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan
Hirohisa Mekata, Satoshi Sekiguchi, Satoru Konnai, Yumi Kirino, Yoichiro Horii, Junzo Norimine
JOURNAL OF VETERINARY MEDICAL SCIENCE, 77, 9, 1115, 1120, Sep. 2015, [Peer-reviewed]
English, Scientific journal

Molecular epidemiological survey and genetic analysis of vector-borne infections of cattle in Luzon Island, the Philippines
Nyamsuren Ochirkhuu, Satoru Konnai, Claro N. Mingala, Tomohiro Okagawa, Marvin Villanueva, Flor Marie Immanuelle R. Pilapil, Shiro Murata, Kazuhiko Ohashi
VETERINARY PARASITOLOGY, 212, 3-4, 161, 167, Sep. 2015, [Peer-reviewed]
English, Scientific journal

組織球性肉腫罹患犬における末梢血中免疫状態の解析　　　　　　　　　　　　　　　
田川 道人, 前川 直也, 今内 覚, 高木 哲
日本獣医学会学術集会講演要旨集, 158回, 407, 407, (公社)日本獣医学会, Aug. 2015
Japanese

Expression of regulatory dendritic cell-related cytokines in cattle experimentally infected with Trypanosoma evansi
Hirohisa Mekata, Shiro Murata, Claro Niegos Mingala, Kazuhiko Ohashi, Satoru Konnai
JOURNAL OF VETERINARY MEDICAL SCIENCE, 77, 8, 1017, 1019, Aug. 2015, [Peer-reviewed]
English, Scientific journal

Vaccination with cyclin-dependent kinase tick antigen confers protection against Ixodes infestation
Helga Gomes, Jorge Moraes, Naftaly Githaka, Renato Martins, Masayoshi Isezaki, Itabajara da Silva Vaz, Carlos Logullo, Satoru Konnai, Kazuhiko Ohashi
VETERINARY PARASITOLOGY, 211, 3-4, 266, 273, Jul. 2015, [Peer-reviewed]
English, Scientific journal

CELLULAR IMMUNE RESPONSE TO INTERFERON-tau IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF JAPANESE BLACK CATTLE WITH BOVINE LEUKEMIA VIRUS INFECTION
Kakinuma Sei-Ichi, Izawa Tomohiro, Matsuda Kei-Ichi, Konnai Satoru, Maeda Yosuke, Ohtsuka Hiromichi
ACTA VETERINARIA-BEOGRAD, 65, 2, 287, 296, Jun. 2015, [Peer-reviewed]
English, Scientific journal

An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes
Yusuke Murase, Satoru Konnai, Shinji Yamada, Naftaly Githaka, Masayoshi Isezaki, Takuya Ito, Ai Takano, Shuji Ando, Hiroki Kawabata, Siro Murata, Kazuhko Ohashi
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 60, 59, 67, May 2015, [Peer-reviewed]
English, Scientific journal

Cytokine mRNA profiling and the proliferative response of bovine peripheral blood mononuclear cells to Mycoplasma bovis
Satoshi Gondaira, Hidetoshi Higuchi, Hidetomo Iwano, Keiichi Nakajima, Kazuhiro Kawai, Shuhei Hashiguchi, Satoru Konnai, Hajime Nagahata
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 165, 1-2, 45, 53, May 2015, [Peer-reviewed]
English, Scientific journal

Evaluation of the natural perinatal transmission of bovine leukaemia virus
Hirohisa Mekata, Satoshi Sekiguchi, Satoru Konnai, Yumi Kirino, Kazuyuki Honkawa, Nariaki Nonaka, Yoichiro Horii, Junzo Norimine
VETERINARY RECORD, 176, 10, 254, +, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Increased expression of the regulatory T cell-associated marker CTLA-4 in bovine leukemia virus infection
Saori Suzuki, Satoru Konnai, Tomohiro Okagawa, Ryoyo Ikebuchi, Asami Nishimori, Junko Kohara, Claro N. Mingala, Shiro Murata, Kazuhiko Ohashi
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 163, 3-4, 115, 124, Feb. 2015, [Peer-reviewed]
English, Scientific journal

Rhipicephalus microplus and Ixodes ovatus cystatins in tick blood digestion and evasion of host immune response
Luis Fernando Parizi, Gabriela Alves Sabadin, Mara Fernanda Alzugaray, Adriana Seixas, Carlos Logullo, Satoru Konnai, Kazuhiko Ohashi, Aoi Masuda, Itabajara da Silva Vaz
PARASITES & VECTORS, 8, 122, Feb. 2015, [Peer-reviewed]
English, Scientific journal

Non-Classical Gluconeogenesis-Dependent Glucose Metabolism in Rhipicephalus microplus Embryonic Cell Line BME26
Renato Martins da Silva, Barbara Della Noce, Camila Fernanda Waltero, Evenilton Pessoa Costa, Leonardo Araujo de Abreu, Naftaly Wang'ombe Githaka, Jorge Moraes, Helga Fernandes Gomes, Satoru Konnai, Itabajara da Silva Vaz, Kazuhiko Ohashi, Carlos Logullo
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16, 1, 1821, 1839, Jan. 2015, [Peer-reviewed]
English, Scientific journal

Bovine immunoinhibitory receptors contribute to suppression of Mycobacterium avium subsp. paratuberculosis-specific T-cell responses
Tomohiro Okagawa, Satoru Konnai, Asami Nishimori, Ryoyo Ikebuchi, Seiko Mizorogi, Reiko Nagata, Satoko Kawaji, Shogo Tanaka, Yumiko Kagawa, Shiro Murata, Yasuyuki Mori, Kazuhiko Ohashi
Infection and Immunity, 84, 1, 77, 89, American Society for Microbiology, 2015, [Peer-reviewed]
English, Scientific journal

北海道のsika deerおよびHaemaphysalis属ダニより検出されたRF Borrelia属(A RF Borrelia sp. found among wild sika deer and Haemaphysalis ticks in Hokkaido)　　　　　　　　　　　　　　　
李 景利, 高野 愛, Taylor Kyle, 佐鹿 万里子, 下鶴 倫人, 今内 覚, 川端 寛樹, 坪田 敏男
日本獣医学会学術集会講演要旨集, 157回, 548, 548, (公社)日本獣医学会, Aug. 2014
English

Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes
Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Kazumasa Yokoyama, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi
IMMUNOLOGY, 142, 4, 551, 561, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirus Marek's disease virus
Ayumi Matsuyama-Kato, Shiro Murata, Masayoshi Isezaki, Sarah Takasaki, Rika Kano, Satoru Konnai, Kazuhiko Ohashi
ARCHIVES OF VIROLOGY, 159, 8, 2123, 2126, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Reprolysin metalloproteases from Ixodes persulcatus, Rhipicephalus sanguineus and Rhipicephalus microplus ticks
Abid Ali, Lucas Tirloni, Masayoshi Isezaki, Adriana Seixas, Satoru Konnai, Kazuhiko Ohashi, Itabajara da Silva Vaz Junior, Carlos Termignoni
EXPERIMENTAL AND APPLIED ACAROLOGY, 63, 4, 559, 578, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Suppressive effects of neutrophil by Salp16-like salivary gland proteins from Ixodes persulcatus Schulze tick
A. Hidano, S. Konnai, S. Yamada, N. Githaka, M. Isezaki, H. Higuchi, H. Nagahata, T. Ito, A. Takano, S. Ando, H. Kawabata, S. Murata, K. Ohahsi
INSECT MOLECULAR BIOLOGY, 23, 4, 466, 474, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Differences in cellular function and viral protein expression between IgM(high) and IgM(low) B-cells in bovine leukemia virus-infected cattle
Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Asami Nishimori, Ayako Nakahara, Shiro Murata, Kazuhiko Ohashi
JOURNAL OF GENERAL VIROLOGY, 95, Pt 8, 1832, 1842, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Human Infections with Borrelia miyamotoi, Japan
Sato, Kozue, Takano, Ai, Konnai, Satoru, Nakao, Minoru, Ito, Takuya, Koyama, Kojiro, Kaneko, Minoru, Ohnishi, Makoto, Kawabata, Hiroki
EMERGING INFECTIOUS DISEASES, 20, 8, 1391, 1393, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Tick Surveillance for Relapsing Fever Spirochete Borrelia miyamotoi in Hokkaido, Japan
Takano, Ai, Toyomane, Kochi, Konnai, Satoru, Ohashi, Kazuhiko, Nakao, Minoru, Ito, Takuya, Andoh, Masako, Maeda, Ken, Watarai, Masahisa, Sato, Kozue, Kawabata, Hiroki
PLOS ONE, 9, 8, e104532, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Identification and characterization of bovine programmed death-ligand 2
Asami Nishimori, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Chie Nakajima, Yasuhiko Suzuki, Claro N. Mingala, Shiro Murata, Kazuhiko Ohashi
MICROBIOLOGY AND IMMUNOLOGY, 58, 7, 388, 397, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-gamma Production from Tumor-Infiltrating Cells by PD-L1 Blockade
Naoya Maekawa, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Mami Adachi, Satoshi Takagi, Yumiko Kagawa, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi
PLOS ONE, 9, 6, e98415, Jun. 2014, [Peer-reviewed]
English, Scientific journal

牛白血病ウイルスに感染した黒毛和種牛の臨床例における免疫状態
伊澤 智宏, 田中 健一郎, 柿沼 清市, 杉山 由夏, 松田 敬一, 小比類巻 正幸, 今内 覚, 大塚 浩通
日本獣医師会雑誌, 67, 5, 328, 332, (公社)日本獣医師会, May 2014
Japanese

Identification and sequence characterization of novel Theileria genotypes from the waterbuck (Kobus defassa) in a Theileria parva-endemic area in Kenya
Naftaly Githaka, Satoru Konnai, Richard Bishop, David Odongo, Isaac Lekolool, Edward Kariuki, Francis Gakuya, Lucy Kamau, Masayoshi Isezaki, Shiro Murata, Kazuhiko Ohashi
VETERINARY PARASITOLOGY, 202, 3-4, 180, 193, May 2014, [Peer-reviewed]
English, Scientific journal

Bovine Leukemia virus titer and leukocyte population associated with mastitis in periparturient dairy cows
Seiichi Kakinuma, Yousuke Maeda, Hiromichi Ohtsuka, Satoru Konnai, Masa-aki Oikawa
INTERNATIONAL JOURNAL OF APPLIED RESEARCH IN VETERINARY MEDICINE, 12, 3, 239, 244, 2014
English, Scientific journal

A relapsing fever group Borrelia sp similar to Borrelia lonestari found among wild sika deer (Cervus nippon yesoensis) and Haemaphysalis spp. ticks in Hokkaido, Japan
Kyunglee Lee, Ai Takano, Kyle Taylor, Mariko Sashika, Michito Shimozuru, Satoru Konnai, Hiroki Kawabata, Toshio Tsubota
TICKS AND TICK-BORNE DISEASES, 5, 6, 841, 847, 2014, [Peer-reviewed]
English, Scientific journal

Sequence characterization and immunogenicity of cystatins from the cattle tick Rhipicephalus (Boophilus) microplus
Luís F. Parizi, Naftaly W. Githaka, Carolina Acevedo, Uruguaysito Benavides, Adriana Seixas, Carlos Logullo, Satoru Konnai, Kazuhiko Ohashi, Aoi Masuda, Itabajara Da Silva Vaz
Ticks and Tick-borne Diseases, 4, 6, 492, 499, Dec. 2013, [Peer-reviewed]
English, Scientific journal

Short communication: Horizontal transmission of bovine leukemia virus from lymphocytotic cattle, and beneficial effects of insect vector control
M. Ooshiro, S. Konnai, Y. Katagiri, M. Afuso, N. Arakaki, O. Tsuha, S. Murata, K. Ohashi
Veterinary Record, 173, 21, 527, 30 Nov. 2013, [Peer-reviewed]
English, Scientific journal

Identification and Structural-Functional Analysis of Cyclin-Dependent Kinases of the Cattle Tick Rhipicephalus (Boophilus) microplus
Helga Gomes, Nelilma C. Romeiro, Gloria R. C. Braz, Eduardo Alves Gamosa de Oliveira, Camilla Rodrigues, Rodrigo Nunes da Fonseca, Naftaly Githaka, Masayoshi Isezaki, Satoru Konnai, Kazuhiko Ohashi, Itabajara da Silva Vaz Jr., Carlos Logullo, Jorge Moraes
PLoS ONE, 8, 10, e76128., 11 Oct. 2013, [Peer-reviewed]
English, Scientific journal

Genotypic variations in field isolates of Theileria species infecting giraffes (Giraffa camelopardalis tippelskirchi and Giraffa camelopardalis reticulata) in Kenya
Naftaly Githaka, Satoru Konnai, Robert Skilton, Edward Kariuki, Esther Kanduma, Shiro Murata, Kazuhiko Ohashi
Parasitology International, 62, 5, 448, 453, Oct. 2013, [Peer-reviewed]
English, Scientific journal

Expression analysis of Foxp3 in T cells from bovine leukemia virus infected cattle
Saori Suzuki, Satoru Konnai, Tomohiro Okagawa, Ryoyo Ikebuchi, Tatsuya Shirai, Yuji Sunden, Claro N. Mingala, Shiro Murata, Kazuhiko Ohashi
Microbiology and Immunology, 57, 8, 600, 604, Aug. 2013, [Peer-reviewed]
English, Scientific journal

Expression analysis of Foxp3 in T-cells from bovine leukemia virus infected cattle.
Suzuki S, Konnai S, Okagawa T, Ikebuchi R, Shirai T, Sunden Y, Mingala CN, Murata S, Ohashi K
Microbiology and immunology, Jun. 2013, [Peer-reviewed]

北海道の野生下におけるボレリア種の維持、小型哺乳類とダニの関係について(On the maintenance of Borrelia spp. among wildlife in Hokkaido: the relationship between small mammals and ticks)　　　　　　　　　　　　　　　
カイル・テイラー, 川端 寛樹, 今内 覚, 下鶴 倫人, 高野 愛, 坪田 敏男
衛生動物, 64, 2, 112, 112, 日本衛生動物学会, Jun. 2013
English

Characterization of Meq proteins from field isolates of Marek's disease virus in Japan
Shiro Murata, Tomoyuki Hashiguchi, Yuko Hayashi, Yuki Yamamoto, Ayumi Matsuyama-Kato, Sarah Takasaki, Masayoshi Isezaki, Misao Onuma, Satoru Konnai, Kazuhiko Ohashi
INFECTION GENETICS AND EVOLUTION, 16, 137, 143, Jun. 2013, [Peer-reviewed]
English, Scientific journal

Differential Tick Burdens May Explain Differential Borrelia afzelii and Borrelia garinii Infection Rates among Four, Wild, Rodent Species in Hokkaido, Japan
Kyle R. Taylor, Ai Takano, Satoru Konnai, Michito Shimozuru, Hiroki Kawabata, Toshio Tsubota
JOURNAL OF VETERINARY MEDICAL SCIENCE, 75, 6, 785, 790, Jun. 2013, [Peer-reviewed]
English, Scientific journal

免疫抑制受容体PD-1のリガンドPD-L2の機能的特徴と臨床応用研究　　　　　　　　　　　　　　　
西森朝美, 今内 覚, 池渕良洋, 岡川朋弘, 村田史郎, 大橋和彦
動物用ワクチンーバイオ医薬品研究会ニュースレター, 7, 19, 20, Jun. 2013, [Invited]
Japanese

Bovine whole-blood culture as a tool for the measurement of endotoxin activities in Gram-negative bacterial vaccines
Saiki Imamura, Mari Nakamizo, Michiko Kawanishi, Nao Nakajima, Kinya Yamamoto, Mariko Uchiyama, Fumiya Hirano, Hidetaka Nagai, Mayumi Kijima, Ryoyo Ikebuchi, Hirohisa Mekata, Shiro Murata, Satoru Konnai, Kazuhiko Ohashi
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 153, 1-2, 153, 158, May 2013, [Peer-reviewed]
English, Scientific journal

Isolation, cloning, and pathologic analysis of Trypanosoma evansi field isolates
Hirohisa Mekata, Satoru Konnai, Claro N. Mingala, Nancy S. Abes, Charito A. Gutierrez, Alan P. Dargantes, William H. Witola, Noboru Inoue, Misao Onuma, Shiro Murata, Kazuhiko Ohashi
PARASITOLOGY RESEARCH, 112, 4, 1513, 1521, Apr. 2013, [Peer-reviewed]
English, Scientific journal

The conserved role of the AKT/GSK3 axis in cell survival and glycogen metabolism in Rhipicephalus (Boophilus) microplus embryo tick cell line BME26
Leonardo Araujo de Abreu, Christiano Calixto, Camila Fernanda Waltero, Barbara Pitta Della Noce, Naftaly Wang'ombe Githaka, Adriana Seixas, Luis Fernando Parizi, Satoru Konnai, Itabajara da Silva Vaz, Kazuhiko Ohashi, Carlos Logullo
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1830, 3, 2574, 2582, Mar. 2013, [Peer-reviewed]
English, Scientific journal

Prevalence of Lyme Borrelia in Ixodes persulcatus Ticks from an Area with a Confirmed Case of Lyme Disease
Yusuke Murase, Satoru Konnai, Naftaly Githaka, Arata Hidano, Kyle Taylor, Takuya Ito, Ai Takano, Shuji Ando, Hiroki Kawabata, Toshio Tsubota, Shiro Murata, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 75, 2, 215, 218, Feb. 2013, [Peer-reviewed]
English, Scientific journal

Borrelia miyamotoi Infections among Wild Rodents Show Age and Month Independence and Correlation with Ixodes persulcatus Larval Attachment in Hokkaido, Japan
Kyle R. Taylor, Ai Takano, Satoru Konnai, Michito Shimozuru, Hiroki Kawabata, Toshio Tsubota
VECTOR-BORNE AND ZOONOTIC DISEASES, 13, 2, 92, 97, Feb. 2013, [Peer-reviewed]
English, Scientific journal

Enhanced expression of LAG-3 on lymphocyte subpopulations from persistently lymphocytotic cattle infected with bovine leukemia virus
Satoru Konnai, Saori Suzuki, Tatsuya Shirai, Ryoyo Ikebuchi, Tomohiro Okagawa, Yuji Sunden, Claro N. Mingala, Misao Onuma, Shiro Murata, Kazuhiko Ohashi
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 36, 1, 63, 69, Jan. 2013, [Peer-reviewed]
English, Scientific journal

Identification and partial characterization of a gut Rhipicephalus appendiculatus cystatin
Saiki Imamura, Satoru Konnai, Shinji Yamada, Luis F. Parizi, Naftaly Githaka, Itabajara da S. Vaz, Shiro Murata, Kazuhiko Ohashi
TICKS AND TICK-BORNE DISEASES, 4, 1-2, 138, 144, 2013, [Peer-reviewed]
English, Scientific journal

Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro
Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Kazumasa Yokoyama, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi
Veterinary Research, 44, 1, 59, 2013, [Peer-reviewed]
English, Scientific journal

The quest for a universal vaccine against ticks: Cross-immunity insights
Luis F. Parizi, Naftaly W. Githaka, Carlos Logullo, Satoru Konnai, Aoi Masuda, Kazuhiko Ohashi, Itabajara da Silva Vaz
VETERINARY JOURNAL, 194, 2, 158, 165, Nov. 2012, [Peer-reviewed]
English, Scientific journal

Molecular detection and characterization of potentially new Babesia and Theileria species/variants in wild felids from Kenya
Naftaly Githaka, Satoru Konnai, Edward Kariuki, Esther Kanduma, Shiro Murata, Kazuhiko Ohashi
ACTA TROPICA, 124, 1, 71, 78, Oct. 2012, [Peer-reviewed]
English, Scientific journal

EFFECTS OF ENVIRONMENTAL LEAD CONTAMINATION ON CATTLE IN A LEAD/ZINC MINING AREA: CHANGES IN CATTLE IMMUNE SYSTEMS ON EXPOSURE TO LEAD IN VIVO AND IN VITRO
Yoshinori Ikenaka, Shouta M. M. Nakayama, Taro Muroya, John Yabe, Satoru Konnai, Wageh Sobhy Darwish, Kaampwe Muzandu, Kennedy Choongo, Geoffrey Mainda, Hiroki Teraoka, Takashi Umemura, Mayumi Ishizuka
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 31, 10, 2300, 2305, Oct. 2012, [Peer-reviewed]
English, Scientific journal

Transcriptional profiling of inflammatory cytokine genes in African, buffaloes (Syncerus caffer) infected with Theileria parva
Tomohiro Okagawa, Satoru Konnai, Hirohisa Mekata, Naftaly Githaka, Saori Suzuki, Edward Kariuki, Francis Gakuya, Esther Kanduma, Tatsuya Shirai, Ryoyo Ikebuchi, Yoshinori Ikenaka, Mayumi Ishizuka, Shiro Murata, Kazuhiko Ohashi
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 148, 3-4, 373, 379, Aug. 2012, [Peer-reviewed]
English, Scientific journal

Effects of interferon-tau on cattle persistently infected with bovine viral diarrhea virus
Junko Kohara, Yumiko Nishikura, Satoru Konnai, Motoshi Tajima, Misao Onuma
JAPANESE JOURNAL OF VETERINARY RESEARCH, 60, 2-3, 63, 70, Aug. 2012, [Peer-reviewed]
English, Scientific journal

Surveillance of Marek's disease virus in migratory and sedentary birds in Hokkaido, Japan
Shiro Murata, Yuko Hayashi, Ayumi Kato, Masayoshi Isezaki, Sarah Takasaki, Misao Onuma, Yuichi Osa, Mitsuhiko Asakawa, Satoru Konnai, Kazuhiko Ohashi
VETERINARY JOURNAL, 192, 3, 538, 540, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Kinetics of regulatory dendritic cells in inflammatory responses during Trypanosoma evansi infection
H. Mekata, S. Konnai, C. N. Mingala, N. S. Abes, C. A. Gutierrez, A. P. Dargantes, W. H. Witola, N. Inoue, M. Onuma, S. Murata, K. Ohashi
PARASITE IMMUNOLOGY, 34, 6, 318, 329, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek's disease virus
Ayumi Matsuyama-Kato, Shiro Murata, Masayoshi Isezaki, Rika Kano, Sara Takasaki, Osamu Ichii, Satoru Konnai, Kazuhiko Ohashi
VIROLOGY JOURNAL, 9, 94, May 2012, [Peer-reviewed]
English, Scientific journal

Increased bovine Tim-3 and its ligand expressions during bovine leukemia virus infection
Tomohiro Okagawa, Satoru Konnai, Ryoyo Ikebuchi, Saori Suzuki, Tatsuya Shirai, Yuji Sunden, Misao Onuma, Shiro Murata, Kazuhiko Ohashi
VETERINARY RESEARCH, 43, 45, May 2012, [Peer-reviewed]
English, Scientific journal

Molecular cloning and characterization of Th1 and Th2 cytokines of African buffalo (Syncerus caffer)
S. Suzuki, S. Konnai, T. Okagawa, N. W. Githaka, E. Kariuki, F. Gakuya, E. Kanduma, T. Shirai, R. Ikebuchi, Y. Ikenaka, M. Ishizuka, S. Murata, K. Ohashi
INTERNATIONAL JOURNAL OF IMMUNOGENETICS, 39, 2, 170, 182, Apr. 2012, [Peer-reviewed]
English, Scientific journal

The Leukocyte Population in the Peripheral Blood and the Colostrum of Cows Infected with Bovine Leukemia Virus is Skewed Towards Humoral Immunity
Seiichi Kakinuma, Yousuke Maeda, Hiromichi Ohtsuka, Kaori Ohmae, Kyouko Ayabe, Satoru Konnai, Masa-aki Oikawa
INTERNATIONAL JOURNAL OF APPLIED RESEARCH IN VETERINARY MEDICINE, 10, 4, 323, 327, 2012, [Peer-reviewed]
English, Scientific journal

Identification of TROSPA homologue in Ixodes persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan
Satoru Konnai, Shinji Yamada, Saiki Imamura, Hideto Nishikado, Naftaly Githaka, Takuya Ito, Ai Takano, Hiroki Kawabata, Shiro Murata, Kazuhiko Ohashi
TICKS AND TICK-BORNE DISEASES, 3, 2, 75, 77, 2012, [Peer-reviewed]
English, Scientific journal

A novel relapsing fever Borrelia sp infects the salivary glands of the molted hard tick, Amblyomma geoemydae
Takano, Ai, Sugimori, Chieko, Fujita, Hiromi, Kadosaka, Teruki, Taylor, Kyle R., Tsubota, Toshio, Konnai, Satoru, Tajima, Tomoko, Sato, Kozue, Watanabe, Haruo, Ohnishi, Makoto, Kawabata, Hiroki
TICKS AND TICK-BORNE DISEASES, 3, 4, 259, 261, 2012, [Peer-reviewed]
English, Scientific journal

Molecular cloning of bovine lymphocyte activation gene-3 and its expression characteristics in bovine leukemia virus-infected cattle
Tatsuya Shirai, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Saori Suzuki, Yuji Sunden, Misao Onuma, Shiro Murata, Kazuhiko Ohashi
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 144, 3-4, 462, 467, Dec. 2011, [Peer-reviewed]
English, Scientific journal

Characterization of reptile-associated Borrelia sp in the vector tick, Amblyomma geoemydae, and its association with Lyme disease and relapsing fever Borrelia spp.
Ai Takano, Hiromi Fujita, Teruki Kadosaka, Satoru Konnai, Tomoko Tajima, Haruo Watanabe, Makoto Ohnishi, Hiroki Kawabata
ENVIRONMENTAL MICROBIOLOGY REPORTS, 3, 5, 632, 637, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade
Ryoyo Ikebuchi, Satoru Konnai, Tatsuya Shirai, Yuji Sunden, Shiro Murata, Misao Onuma, Kazuhiko Ohashi
VETERINARY RESEARCH, 42, 103, Sep. 2011, [Peer-reviewed]
English, Scientific journal

Analysis of transcriptional activities of the Meq proteins present in highly virulent Marek's disease virus strains, RB1B and Md5
Shiro Murata, Tsukasa Okada, Rika Kano, Yuko Hayashi, Tomoyuki Hashiguchi, Misao Onuma, Satoru Konnai, Kazuhiko Ohashi
VIRUS GENES, 43, 1, 66, 71, Aug. 2011, [Peer-reviewed]
English, Scientific journal

Molecular detection of Anaplasma phagocytophilum in cattle and Ixodes persulcatus ticks
Yusuke Murase, Satoru Konnai, Arata Hidano, Naftali W. Githaka, Takuya Ito, Ai Takano, Hiroki Kawabata, Manabu Ato, Tomoko Tajima, Motoshi Tajima, Misao Onuma, Shiro Murata, Kazuhiko Ohashi
VETERINARY MICROBIOLOGY, 149, 3-4, 504, 507, May 2011, [Peer-reviewed]
English, Scientific journal

Multilocus Sequence Typing Implicates Rodents as the Main Reservoir Host of Human-Pathogenic Borrelia garinii in Japan
Ai Takano, Minoru Nakao, Toshiyuki Masuzawa, Nobuhiro Takada, Yasuhiro Yano, Fubito Ishiguro, Hiromi Fujita, Takuya Ito, Xiaohang Ma, Yozaburo Oikawa, Fumihiko Kawamori, Kunihiko Kumagai, Toshiyuki Mikami, Nozomu Hanaoka, Shuji Ando, Naoko Honda, Kyle Taylor, Toshio Tsubota, Satoru Konnai, Haruo Watanabe, Makoto Ohnishi, Hiroki Kawabata
JOURNAL OF CLINICAL MICROBIOLOGY, 49, 5, 2035, 2039, May 2011, [Peer-reviewed]
English, Scientific journal

Molecular identification and expression analysis of lipocalins from blood feeding taiga tick, Ixodes persulcatus Schulze
Satoru Konnai, Hideto Nishikado, Shinji Yamada, Saiki Imamura, Takuya Ito, Misao Onuma, Shiro Murata, Kazuhiko Ohashi
EXPERIMENTAL PARASITOLOGY, 127, 2, 467, 474, Feb. 2011, [Peer-reviewed]
English, Scientific journal

Peripheral Blood Leukocyte Populations in Dairy Cows Infected with Bovine Leukemia Virus
Seiichi KAKINUMA, Hiromichi OHTSUKA, Kaori OHMAE, Kyouko AYABE, Motoharu KAKINUMA, Satoru KONNAI, Masaaki OIKAWA
Journal of the Japan Veterinary Medical Association, 64, 5, 375, 380, Japan Veterinary Medical Association, 2011, [Peer-reviewed]
Scientific journal

Characterization of CTLA-4, PD-1 and PDL-1 of swamp and riverine type water buffaloes
Claro N. Mingala, Satoru Konnai, Ryoyo Ikebuchi, Kazuhiko Ohashi
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 34, 1, 55, 63, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Two novel Salp15-like immunosuppressant genes from salivary glands of Ixodes persulcatus Schulze tick
A. Mori, S. Konnai, S. Yamada, A. Hidano, Y. Murase, T. Ito, A. Takano, H. Kawabata, M. Onuma, K. Ohashi
INSECT MOLECULAR BIOLOGY, 19, 3, 359, 365, Jun. 2010, [Peer-reviewed]
English, Scientific journal

Molecular cloning and expression analysis of bovine programmed death-1
Ryoyo Ikebuchi, Satoru Konnai, Yuji Sunden, Misao Onuma, Kazuhiko Ohashi
MICROBIOLOGY AND IMMUNOLOGY, 54, 5, 291, 298, May 2010, [Peer-reviewed]
English, Scientific journal

Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6
Hirohisa Mekata, Satoru Konnai, William H. Witola, Noboru Inoue, Misao Onuma, Kazuhiko Ohashi
INFECTION GENETICS AND EVOLUTION, 9, 6, 1301, 1305, Dec. 2009, [Peer-reviewed]
English, Scientific journal

PCR-based detection of blood parasites in cattle and adult Rhipicephalus appendiculatus ticks
Shinji Yamada, Satoru Konnai, Saiki Imamura, Martin Simuunza, Mwelwa Chembensofu, Amos Chota, Andrew Nambota, Misao Onuma, Kazuhiko Ohashi
VETERINARY JOURNAL, 182, 2, 352, 355, Nov. 2009, [Peer-reviewed]
English, Scientific journal

Quantification of water buffalo (Bubalus bubalis) cytokine expression in response to inactivated foot-and-mouth disease (FMD) vaccine
Claro N. Mingala, Satoru Konnai, Fe A. Venturina, Misao Onuma, Kazubliko Ohashi
RESEARCH IN VETERINARY SCIENCE, 87, 2, 213, 217, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Classification of new BVDV isolates from Philippine water buffalo using the viral E2 region
Claro N. Mingala, Satoru Konnai, Motoshi Tajima, Misao Onuma, Kazuhiko Ohashi
JOURNAL OF BASIC MICROBIOLOGY, 49, 5, 495, 500, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Cloning and characterization of Rhipicephalus appendiculatus voraxin alpha and its effect as anti-tick vaccine
Shinji Yamada, Satoru Konnai, Saiki Imamura, Takuya Ito, Misao Onuma, Kazuhiko Ohashi
VACCINE, 27, 43, 5989, 5997, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis
Saiki Imamura, Itabajara da Silva Vaz, Satoru Konnai, Shinji Yamada, Chie Nakajima, Misao Onuma, Kazuhiko Ohashi
EXPERIMENTAL AND APPLIED ACAROLOGY, 48, 4, 345, 358, Aug. 2009, [Peer-reviewed]
English, Scientific journal

Identification and characterization of antimicrobial peptide, defensin, in the taiga tick, Ixodes persulcatus
Y. Saito, S. Konnai, S. Yamada, S. Imamura, H. Nishikado, T. Ito, M. Onuma, K. Ohashi
INSECT MOLECULAR BIOLOGY, 18, 4, 531, 539, Aug. 2009, [Peer-reviewed]
English, Scientific journal

Development and application of a quantitative real-time PCR for the diagnosis of Surra in water buffaloes
Satoru Konnai, Hirohisa Mekata, Claro N. Mingala, Nancy S. Abes, Charito A. Gutierrez, Jesus Rommel V. Herrera, Alan P. Dargantes, William H. Witola, Libertado C. Cruz, Noboru Inoue, Misao Onuma, Kazuhiko Ohashi
INFECTION GENETICS AND EVOLUTION, 9, 4, 449, 452, Jul. 2009, [Peer-reviewed]
English, Scientific journal

Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus
Carlos Logullo, William H. Witola, Caroline Andrade, Leonardo Abreu, Josiana Gomes, Itabajara da Silva Vaz, Saiki Imamura, Satoru Konnai, Kazuhiko Ohashi, Misao Onuma
VETERINARY PARASITOLOGY, 161, 3-4, 261, 269, May 2009, [Peer-reviewed]
English, Scientific journal

Comparative moleculo-immunological analysis of swamp- and riverine-type water buffaloes responses
Claro N. Mingala, Satoru Konnai, Libertado C. Cruz, Misao Onuma, Kazuhiko Ohashi
CYTOKINE, 46, 2, 273, 282, May 2009, [Peer-reviewed]
English, Scientific journal

Microarray Analysis of Host Immune Responses to Marek's Disease Virus Infection in Vaccinated Chickens
Rika Kano, Satoru Konnai, Misao Onuma, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 71, 5, 603, 610, May 2009, [Peer-reviewed]
English, Scientific journal

Cytokine profiles in chickens infected with virulent and avirulent Marek's disease viruses: Interferon-gamma is a key factor in the protection of Marek's disease by vaccination
Rika Kano, Satoru Konnai, Misao Onuma, Kazuhiko Ohashi
MICROBIOLOGY AND IMMUNOLOGY, 53, 4, 224, 232, Apr. 2009, [Peer-reviewed]
English, Scientific journal

Suppression of cell proliferation and cytokine expression by HL-p36, a tick salivary gland-derived protein of Haemaphysalis longicornis
Satoru Konnai, Chie Nakajima, Saiki Imamura, Shinji Yamada, Hideto Nishikado, Michi Kodama, Misao Onuma, Kazuhiko Ohashi
IMMUNOLOGY, 126, 2, 209, 219, Feb. 2009, [Peer-reviewed]
English, Scientific journal

Quantitative Analysis of Cytokine mRNA Expression and Protozoan DNA Load in Theileria parva-Infected Cattle
Shinji Yamada, Satoru Konnai, Saiki Imamura, Martin Simuunza, Mwelwa Chembensofu, Amos Chota, Andrew Nambota, Misao Onuma, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 71, 1, 49, 54, Jan. 2009, [Peer-reviewed]
English, Scientific journal

Rhipicephalus appendiculatus: Characterization of a testis-associated protein
Shinji Yamada, Yuko Ito, Saiki Imamura, Satoru Konnai, Takuya Ito, Misao Onuma, Kazuhiko Ohashi
EXPERIMENTAL PARASITOLOGY, 120, 4, 337, 342, Dec. 2008, [Peer-reviewed]
English, Scientific journal

Prevalence and Source of Trypanosome Infections in Field-Captured Vector Flies (Glossina pallidipes) in Southeastern Zambia
Hirohisa Mekata, Satoru Konnai, Martin Simuunza, Mwelwa Chembensofu, Rika Kano, William H. Witola, Mwase E. Tembo, Harrison Chitambo, Noboru Inoue, Misao Onuma, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 70, 9, 923, 928, Sep. 2008
English, Scientific journal

Prevalence and Source of Trypanosome Infections in Field-Captured Vector Flies (Glossina pallidipes) in Southeastern Zambia
Hirohisa Mekata, Satoru Konnai, Martin Simuunza, Mwelwa Chembensofu, Rika Kano, William H. Witola, Mwase E. Tembo, Harrison Chitambo, Noboru Inoue, Misao Onuma, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 70, 9, 923, 928, Sep. 2008, [Peer-reviewed]
English, Scientific journal

Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies
Satoru Konnai, Hirohisa Mekata, Raadan Odbileg, Martin Simuunza, Mwelwa Chembensof, William Harold Witola, Mwase Enala Tembo, Harrison Chitambo, Noboru Inoue, Misao Onuma, Kazuhiko Ohashi
VECTOR-BORNE AND ZOONOTIC DISEASES, 8, 4, 565, 573, Aug. 2008, [Peer-reviewed]
English, Scientific journal

Serological survey of Toxoplasma gondii in wild waterfowl in Chukotka, Kamchatka, Russia and Hokkaido, Japan
Takehiro Murao, Yoshitaka Omata, Rika Kano, Shiro Murata, Tsukasa Okada, Satoru Konnai, Mitsuhiko Asakawa, Kazuhiko Ohashi, Misao Onuma
JOURNAL OF PARASITOLOGY, 94, 4, 830, 833, Aug. 2008, [Peer-reviewed]
English, Scientific journal

Effects of anti-tick cocktail vaccine against Rhipicephalus appendiculatus
Saiki Imamura, Satoru Konnai, Itabajara da Silva Vaz Junior, Shinji Yamada, Chie Nakajima, Yuko Ito, Tomoko Tajima, Jun Yasuda, Martin Simuunza, Misao Onuma, Kazuhiko Ohashi
JAPANESE JOURNAL OF VETERINARY RESEARCH, 56, 2, 85, 98, Aug. 2008, [Peer-reviewed]
English, Scientific journal

A survey of abortifacient infectious agents in livestock in Luzon, the Philippines, with emphasis on the situation in a cattle herd with abortion problems
Satoru Konnai, Claro N. Mingala, Misako Sato, Nancy S. Abes, Fe A. Venturina, Charito A. Gutierrez, Takafumi Sano, Yoshitaka Omata, Libertado C. Cruz, Misao Onuma, Kazuhiko Ohashi
ACTA TROPICA, 105, 3, 269, 273, Mar. 2008, [Peer-reviewed]
English, Scientific journal

Cytokine responses in camels (Camelus bactrianus) vaccinated with Brucella abortus strain 19 vaccine
Raadan Odbileg, Byambaa Purevtseren, Dorj Gantsetseg, Bazartseren Boldbaatar, Tumurjav Buyannemekh, Zagd Galmandakh, Janchivdorj Erdenebaatar, Satoru Konnai, Misao Onuma, Kazuhiko Ohashi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 70, 2, 197, 201, Feb. 2008, [Peer-reviewed]
English, Scientific journal

Establishment of a laboratory colony of taiga tick Ixodes persulcatus for tick-borne pathogen transmission studies
Satoru Konnai, Yoichi Saito, Hideto Nishikado, Shinji Yamada, Saiki Imamura, Akina Mori, Takuya Ito, Misao Onuma, Kazuhiko Ohashi
JAPANESE JOURNAL OF VETERINARY RESEARCH, 55, 2-3, 85, 92, Jan. 2008, [Peer-reviewed]
English, Scientific journal

Development of a nested polymerase chain reaction method to detect oncogenic Marek's disease virus from feather tips
Shiro Murata, Kyung-Soo Chang, Sung-Il Lee, Satoru Konnai, Misao Onuma, Kazuhiko Ohashi
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 19, 5, 471, 478, Sep. 2007, [Peer-reviewed]
English, Scientific journal

Detection of the virulent Marek's disease virus genome from feather tips of wild geese in Japan and the Far East region of Russia
S. Murata, K.-S. Chang, Y. Yamamoto, T. Okada, S.-I. Lee, S. Konnai, M. Onuma, Y. Osa, M. Asakawa, K. Ohashi
ARCHIVES OF VIROLOGY, 152, 8, 1523, 1526, Aug. 2007, [Peer-reviewed]
English, Scientific journal

Attachment duration required for Rhipicephalus appendiculatus to transmit Theileria parva to the host
Satoru Konnai, Shinji Yamada, Saiki Imamura, Martin Simuunza, Mwelwa Chembensof, Amos Chota, Andrew Nambota, Kazuhiko Ohashi, Misao Onuma
Vector-Borne and Zoonotic Diseases, 7, 2, 241, 248, Jun. 2007, [Peer-reviewed]
English, Scientific journal

Molecular cloning, sequencing and phylogenetic analysis of inflammatory cytokines of swamp type buffalo contrasting with other bubaline breeds
Claro N. Mingala, Raadan Odbileg, Satoru Konnai, Kazuhiko Ohashi, Misao Onuma
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 30, 2, 119, 131, Mar. 2007, [Peer-reviewed]
English, Scientific journal

Interferon-gamma expression associated with suppression of bovine leukemia virus at the early phase of infection in sheep
Tatsufumi Usui, Satoru Konnai, Kazuhiko Ohashi, Misao Onuma
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 115, 1-2, 17, 23, Jan. 2007, [Peer-reviewed]
English, Scientific journal

Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics
Claro N. Mingala, Raadan Odbileg, Satoru Konnal, Kazuhiko Ohashi, Misao Onuma
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 113, 3-4, 348, 356, Oct. 2006, [Peer-reviewed]
English, Scientific journal

Complete cDNA sequences and phylogenetic analyses of the Th1 and Th2 cytokines of the bactrian camel (Camelus bactrianus)
Raadan Odbileg, Byambaa Purevtseren, Zayat Batsukh, Satoru Konnaii, Kazuhiko Ohashi, Misao Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, 9, 941, 946, Sep. 2006, [Peer-reviewed]
English, Scientific journal

Expression of tumor necrosis factor-alpha in IgM(+) B-cells from bovine leukemia virus-infected lymphocytotic sheep
Tatsufumi Usui, Satoru Konnai, Kazuhiko Ohashi, Misao Onuma
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 112, 3-4, 296, 301, Aug. 2006, [Peer-reviewed]
English, Scientific journal

Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus
Satoru Konnai, Saiki Imamura, Chie Nakajima, William Harold Witola, Shinji Yamada, Martin Simuunza, Andrew Nambota, Jun Yasuda, Kazuhiko Ohashi, Misao Onuma
ACTA TROPICA, 99, 1, 34, 41, Aug. 2006, [Peer-reviewed]
English, Scientific journal

Tumor necrosis factor-alpha genetic polymorphism may contribute to progression of bovine leukemia virus-infection
Satoru Konnai, Tatsufumi Usui, Manabu Ikeda, Junko Kohara, Toh-ichi Hirata, Kosuke Okada, Kazuhiko Ohashi, Misao Onuma
MICROBES AND INFECTION, 8, 8, 2163, 2171, Jul. 2006, [Peer-reviewed]
English, Scientific journal

The effect of TAO expression on PCD-like phenomenon development and drug resistance in Trypanosoma brucei
A Tsuda, WH Witola, S Konnai, K Ohashi, M Onuma
PARASITOLOGY INTERNATIONAL, 55, 2, 135, 142, Jun. 2006, [Peer-reviewed]
English, Scientific journal

A novel gene encoding a thrombin inhibitory protein in a cDNA library from Haemaphysalis longicornis salivary gland
C Nakajima, S Imamura, S Konnai, S Yamada, H Nishikado, K Ohashi, M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, 5, 447, 452, May 2006, [Peer-reviewed]
English, Scientific journal

Experimental transmission of Bovine leukemia virus in cattle via rectal palpation
Junko Kohara, Satoru Konnai, Misao Onuma
JAPANESE JOURNAL OF VETERINARY RESEARCH, 54, 1, 25, 30, May 2006, [Peer-reviewed]
English, Scientific journal

Tumor necrosis factor-alpha up-regulation in spontaneously proliferating cells derived from bovine leukemia virus-infected cattle
S Konnai, T Usui, M Ikeda, J Kohara, T Hirata, K Okada, K Ohashi, M Onuma
ARCHIVES OF VIROLOGY, 151, 2, 347, 360, Feb. 2006, [Peer-reviewed]
English, Scientific journal

Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis
HM Pham, S Konnai, T Usui, KS Chang, S Murata, M Mase, K Ohashi, M Onuma
ARCHIVES OF VIROLOGY, 150, 12, 2429, 2438, Dec. 2005, [Peer-reviewed]
English, Scientific journal

Bovine leukemia virus high tax molecular clone experimentally induces leukemia/lymphoma in sheep
K Okada, N Nakae, K Kuramochi, S Yin, M Ikeda, S Takami, T Hirata, M Goryo, S Numakunai, S Takeshima, M Takahashi, S Tajima, S Konnai, M Onuma, Y Aida
JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 12, 1231, 1235, Dec. 2005, [Peer-reviewed]
English, Scientific journal

Random sequencing of cDNA library derived from partially-fed adult female Haemaphysalis longicornis salivary gland
C Nakajima, ID Vaz, S Imamura, S Konnai, K Ohashi, M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 11, 1127, 1131, Nov. 2005, [Peer-reviewed]
English, Scientific journal

Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection
S Konnai, T Usui, M Ikeda, J Kohara, T Hirata, K Okada, K Ohashi, M Onuma
VIROLOGY, 339, 2, 239, 248, Sep. 2005, [Peer-reviewed]
English, Scientific journal

Molecular cloning and phylogenetic analysis of inflammatory cytokines of Camelidae (llama and camel)
R Odbileg, S Konnai, K Ohashi, M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 9, 921, 925, Sep. 2005, [Peer-reviewed]
English, Scientific journal

Immmohistochemical analysis of expression patterns of tumor necrosis factor receptors on lymphoma cells in enzootic bovine leukosis
M Ikeda, S Konnai, M Onuma, N Ishiguro, M Goryo, K Okada
JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 4, 425, 432, Apr. 2005, [Peer-reviewed]
English, Scientific journal

Quantification of llama inflammatory cytokine mRNAs by real-time RT-PCR
R Odbileg, S Konnai, T Usui, K Ohashi, M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 2, 195, 198, Feb. 2005, [Peer-reviewed]
English, Scientific journal

Ex vivo survival of peripheral blood mononuclear cells in sheep induced by bovine leukemia virus (BLV) mainly occurs in CD5- B cells that express BLV
Masahiko Takahashi, Shigeru Tajima, Shin-Nosuke Takeshima, Satoru Konnai, Shan Ai Yin, Kosuke Okada, William C. Davis, Yoko Aida
Microbes and Infection, 6, 6, 584, 595, May 2004, [Peer-reviewed]
English, Scientific journal

Protective effects of vaccination with bovine leukemia virus (BLV) tax DNA against BLV infection in sheep
T Usui, S Konnai, S Tajima, S Watarai, Y Aida, K Ohashi, M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 65, 11, 1201, 1205, Nov. 2003, [Peer-reviewed]
English, Scientific journal

Technical note: DNA typing for ovine MHC DRB1 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
S Konnai, Y Nagaoka, S Takesima, M Onuma, Y Aida
JOURNAL OF DAIRY SCIENCE, 86, 10, 3362, 3365, Oct. 2003, [Peer-reviewed]
English, Scientific journal

Sequences and diversity of 17 new Ovar-DRB1 alleles from three breeds of sheep
S Konnai, Y Nagaoka, S Takeshima, M Onuma, Y Aida
EUROPEAN JOURNAL OF IMMUNOGENETICS, 30, 4, 275, 282, Aug. 2003, [Peer-reviewed]
English, Scientific journal

The rapid quantitative analysis of bovine cytokine genes by real-time RT-PCR
S Konnai, T Usui, K Ohashi, M Onuma
VETERINARY MICROBIOLOGY, 94, 4, 283, 294, Jul. 2003, [Peer-reviewed]
English, Scientific journal

牛白血病ウイルス(BLV)Envに対する抗体反応性とウシMHCクラスII DR抗原との相関性　　　　　　　　　　　　　　　
竹嶋 伸之輔, 嘉村 浩美, 若本 裕晶, 田島 茂, 今内 覚, 森田 裕, 小沼 操, 岡田 幸助, 間 陽子
日本獣医学会学術集会講演要旨集, 135回, 123, 123, (公社)日本獣医学会, Mar. 2003
Japanese

A mutant form of the Tax protein of bovine leukemia virus (BLV), with enhanced transactivation activity, increases expression and propagation of BLV in vitro but not in vivo
S Tajima, M Takahashi, S Takeshima, S Konnai, SA Yin, S Watarai, Tana, Y Tanaka, M Onuma, K Okada, Y Aida
JOURNAL OF VIROLOGY, 77, 3, 1894, 1903, Feb. 2003, [Peer-reviewed]
English, Scientific journal

Seroprevalence of bovine immunodeficiency virus and bovine leukemia virus in dairy and beef cattle in Hokkaido
T Usui, S Meas, S Konnai, K Ohashi, M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 65, 2, 287, 289, Feb. 2003, [Peer-reviewed]
English, Scientific journal

The influence of ovine MHC class II DRB1 alleles on immune response in bovine leukemia virus infection
S Konnai, NS Takeshima, S Tajima, SA Yin, K Okada, M Onuma, Y Aida
MICROBIOLOGY AND IMMUNOLOGY, 47, 3, 223, 232, 2003, [Peer-reviewed]
English, Scientific journal

Effects of chlorpromazine, pentoxifylline and dexamethasone on mRNA expression of lipopolysaccharide-induced inflammatory cytokines in bovine peripheral blood mononuclear cells
Y Kiku, H Matsuzawa, H Ohtsuka, N Terasaki, S Fukuda, S Kon-Nai, M Koiwa, Y Yokomizo, H Sato, TJ Rosol, H Okada, TO Yoshino
JOURNAL OF VETERINARY MEDICAL SCIENCE, 64, 8, 723, 726, Aug. 2002, [Peer-reviewed]
English, Scientific journal

BLV-Tax発現DNAワクチンのウイルス増殖抑制効果　　　　　　　　　　　　　　　
笛吹 達史, 今内 覚, 田島 茂, 渡来 仁, 大橋 和彦, 間 陽子, 小沼 操
日本獣医学会学術集会講演要旨集, 133回, 95, 95, (公社)日本獣医学会, Mar. 2002
Japanese

ヒツジMHC(OLA)-DRB1の塩基配列の決定と多型解析
今内 覚, 長岡 淑子, 小沼 操, 間 陽子
MHC: Major Histocompatibility Complex, 8, 2, 122, 122, (一社)日本組織適合性学会, Sep. 2001
Japanese

異なるMHCを有するBLV感染羊の免疫応答
今内 覚, 竹嶋 伸之輔, 田島 茂, 岡田 幸助, 小沼 操, 間 陽子
MHC: Major Histocompatibility Complex, 8, 2, 136, 136, (一社)日本組織適合性学会, Sep. 2001
Japanese

Inflammatory Cytokines and Antigen-Responsive Mononuclear Cells in Peripheral Blood of Cattle Infected with Salmonella Takoradi
KONNAI Satoru, OHTSUKA Hiromichi, SAKAMOTO Tetsuaki, NAKAOKA Yuji, KIKU Yukio, FUKUDA Sigeo, KOIWA Masateru, TAKAHASHI Junkichi, TANIYAMA Hiroyuki, YOKOMIZO Yuichi, OKADA Hiroyuki, YOSHINO Tomoo
Journal of Veterinary Medical Science, 63, 8, 859, 865, Japanese Society of Veterinary Science, 25 Aug. 2001, [Peer-reviewed]
English, Scientific journal, To determine the immunological response in lactating dairy cows infected with Salmonella (S.) Takoradi, the relationships among distributions of peripheral blood mononuclear cell (PBMC) subpopulations, endotoxin concentrations and dynamics of inflammatory cytokines in blood were investigated. The ratio of CD4⁺ T cells to CD8⁺ T cells was significantly lower in the affected cattle than in the control cattle (p<0.05) to decrease in the number of CD4⁺ T cells in the infected cattle. In contrast, the numbers of γδ-T cells, MHC class II-positive cells were significantly higher in the affected cattle than in the control cattle (p<0.01 respectively). Endotoxemia was found in all but one of the affected cattle. Serum IL-1 and IL-6 bioactivities were significantly higher in the affected cattle than in the control cattle (IL-1, p<0.05; IL-6, p<0.01). Serum TNF-α activities and levels were not detected in the control and affected cattle. The activities of proinflammatory cytokines determined by the bioassay are important to the relationships between concentration of endotoxin, cytokines and clinical signs, such as leukocytosis, leukopenia, fever or bacterial shedding. Serum IL-2 levels were lower in the affected cattle than in the control cattle. Serum IFN-γ was not detected in the affected cattle except one. These results by the ELISA seemed to reflect the condition of subpopulation in the PBMCs from the shedding cattle. The present results suggest that cellular immunity is suppressed while the humoral immunity is activated in acute bovine salmonellosis.

牛白血病ウイルス(BLV)Tax変異体をコードする感染性分子クローン接種ヒツジにおけるウイルス動態の解析　　　　　　　　　　　　　　　
田島 茂, 今内 覚, 竹嶋 伸之輔, 高橋 雅彦, 渡来 仁, 小沼 操, 岡田 幸助, 間 陽子
日本獣医学会学術集会講演要旨集, 131回, 84, 84, (公社)日本獣医学会, Mar. 2001
Japanese

ウシ白血病ウイルス(BLV)感染初期羊におけるCD4+T細胞のBLV抗原に対する反応性　　　　　　　　　　　　　　　
尹 善愛, 池田 学, 御領 政信, 沼宮内 茂, 平田 統一, 今内 覚, 小沼 操, 間 陽子, 岡田 幸助
日本獣医学会学術集会講演要旨集, 131回, 92, 92, (公社)日本獣医学会, Mar. 2001
Japanese

異なるMHCを有するBLV感染羊の免疫応答　　　　　　　　　　　　　　　
今内 覚, 竹嶋 伸之輔, 田島 茂, 岡田 幸助, 小沼 操, 間 陽子
日本獣医学会学術集会講演要旨集, 131回, 92, 92, (公社)日本獣医学会, Mar. 2001
Japanese

Effects of Lipopolysaccharide on Production of Interleukin-1 and Interleukin-6 by Bovine Mammary Epithelial Cells in vitro
Hiroyuki Okada, Hiromichi Ohtsuka, Satoru Kon-Nai, Rikio Kirisawa, Yuichi Yokomizo, Tomo-O. Yoshino, Thomas J. Rosol
Journal of Veterinary Medical Science, 61, 1, 33, 35, Jan. 1999, [Peer-reviewed]
English, Scientific journal

Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro
H Okada, H Ohtsuka, S Kon-Nai, R Kirisawa, Y Yokomizo, T Yoshino, TJ Rosol
JOURNAL OF VETERINARY MEDICAL SCIENCE, 61, 1, 33, 35, Jan. 1999, [Peer-reviewed]
English, Scientific journal

特集：牛伝染性リンパ腫の対応と取り組み・牛伝染性リンパ腫の現状と早期発症予測法　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 臨床獣医, 43, 13, 24, 28, Dec. 2025, [Invited], [Lead author, Corresponding author]
Japanese, Report scientific journal

視点2025・最新技術が映す酪農の未来：ハイリスク牛摘発と優先淘汰進め、畜産被害軽減と生産性向上 牛リンパ腫早期診断技術用いた「がん検診」の可能性　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, DAIRYMAN, 75, 11, 26, 29, Nov. 2025, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛伝染性リンパ腫発症を予測するがん検診技術　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 宮城県獣医師会会報, 78, 4, 120, 126, Oct. 2025, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

現場で活かす酪農技術 ’牛のがん検診’技術が現場の損失を救う！　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, Dairy Japan, 70, 11, 10, 14, Sep. 2025, [Invited], [Lead author, Corresponding author]
Japanese, Introduction international proceedings

牛伝染性リンパ腫の現状と早期発症予測法　　　　　　　　　　　　　　　
今内 覚, J. Vet. Epidemiol., 28, 2, 95, 100, Dec. 2024, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛のリンパ腫発症を予測するがん検診技術　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 畜産技術, 834, 24, 29, Nov. 2024, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

子牛用ワクチン効果の新規増強法の試み　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 臨床獣医, 42, 2, 38, 41, Feb. 2024, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

Effect of polymorphisms in the meq gene of Marek’s disease virus distributed in Japan on the pathogenicity and transcriptional regulation
もたい吉之介, 村田史郎, 村田史郎, 佐藤純平, 山上竣裕, SHWE Win Yee, 堀尾文彌, 前川直也, 岡川朋弘, 今内覚, 今内覚, 今内覚, 大橋和彦, 大橋和彦, 大橋和彦, 日本ウイルス学会学術集会プログラム・予稿集(Web), 71st, 2024

Effect of polymorphisms in the basic region of Meq protein of Marek’s disease, an avian oncogenic herpesvirus, on virulence
佐藤純平, 村田史郎, 村田史郎, もたい吉之介, 山上竣裕, 堀尾文彌, SHWE Win Yee, 前川直也, 岡川朋弘, 今内覚, 今内覚, 今内覚, 大橋和彦, 大橋和彦, 大橋和彦, 日本ウイルス学会学術集会プログラム・予稿集(Web), 71st, 2024

非典型的な地方病性牛伝染性リンパ腫症例のRAISING法を用いた性状解析
松原幸芽, 岡川朋弘, 斎藤益満, 松平崇弘, 直亨則, 神谷可菜, 齋藤麻矢, 前川直也, 村田史郎, 大橋和彦, 今内覚, 日本獣医学会学術集会講演要旨集, 167th, 2024

抗PD-L1抗体により治療した悪性黒色腫の犬における血清バイオマーカーの探索
飯高早紀子, 前川直也, 今内覚, 出口辰弥, 岡川朋弘, 高木哲, 高木哲, 鈴木定彦, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 167th, 2024

プロバイオティクスによる子牛のワクチン効果増強作用の検証
池端麻里, 岡川朋弘, 中村隼人, 前川直也, 村田史郎, 大橋和彦, 今内覚, 今内覚, 日本獣医学会学術集会講演要旨集, 167th, 2024

日本に分布するマレック病ウイルスmeq遺伝子による病原性への影響
もたい吉之介, 村田史郎, 佐藤純平, 山上竣裕, WIN Shwe Yee, 堀尾文彌, 前川直也, 岡川朋弘, 今内覚, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 167th, 2024

ワクモ曝露型抗原による抗ワクモワクチン抗原としての評価
堀尾文彌, 村田史郎, ウィン シュウェ・イー, 佐藤純平, もたい吉之介, 山上竣裕, 佐藤匠, 大石英司, 前川直也, 岡川朋弘, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 167th, 2024

マレック病ウイルスmeq遺伝子の転写活性化調節ドメインの多型と病原性との関連
山上竣裕, 村田史郎, 佐藤純平, もたい吉之介, WIN Shwe Yee, 堀尾文彌, 前川直也, 岡川朋弘, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 167th, 2024

小動物臨床最前線！ 二次診療の現場より　　　　　　　　　　　　　　　
今内 覚, 北小獣通信, 1, 93, Jan. 2024, [Invited], [Lead author]
Japanese, Introduction scientific journal

One HealthアプローチにもとづくBLV/HTLV-Ⅰ感染症におけるリンパ腫の診断と早期発症予測法の開発　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 斎藤益満, 臨床とウイルス, 51, 4, 192, 198, Oct. 2023, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛伝染性リンパ腫診断の新技術・早期発症予測診断法RAISINGとは　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 家畜診療, 70, 5, 259, 266, May 2023, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

成果紹介：ウシ伝染性リンパ腫の早期発症診断による経済損失低減の実現　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, JATAFFジャーナル, 11, 4, 45, 50, Apr. 2023, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
Japanese, Report scientific journal

研究紹介：牛のリンパ腫発症を予測するがん検診技術を開発 ～発症予測法の実用化による畜産被害の軽減に期待～　　　　　　　　　　　　　　　
今内 覚, 北海道獣医師会誌, 67, 44, 47, Feb. 2023, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛伝染性リンパ腫の現状から最後の砦：発症予測法について　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 臨床獣医, 41, 2, 46, 31, Feb. 2023, [Invited], [Lead author, Corresponding author]
Japanese, Report scientific journal

ピックアップ新技術：牛のリンパ腫発症を予測するがん検診技術を開発. リスク高い牛の優先淘汰による発生予防に期待　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, DAIRYMAN, 73, 2, 64, 66, Feb. 2023, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛伝染性リンパ腫の診断と早期発症予測法の開発
富永みその, 今内覚, 岡川朋弘, 神谷可菜, 齋藤麻矢, 安富一郎, 目堅博久, 前川直也, 村田史郎, 大橋和彦, 北海道獣医師会雑誌, 67, 8, 2023

免疫チェックポイント分子Programmed death-ligand1(PD-L1)を標的とした抗体薬による犬の鼻腔内腺癌に対する免疫療法の検討
多田佳史, 前川直也, 今内覚, 細谷謙次, 大脇稜, 竹内寛人, 賀川由美子, 高木哲, 高木哲, 鈴木定彦, 鈴木定彦, 岡川朋弘, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 166th, 2023

牛伝染性リンパ腫ウイルス感染症における免疫チェックポイント因子TIM-3およびPD-L1を標的とした抗ウイルス効果の検討
中村隼人, 今内覚, 岡川朋弘, 前川直也, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 166th, 2023

クローナリティ解析による地方病性牛伝染性リンパ腫に対する発症予測法の開発
富永みその, 岡川朋弘, 嶋倉穂南, 斎藤益満, 松平崇弘, 直亨則, 山田慎二, 村上賢二, 前川直也, 村田史郎, 大橋和彦, 今内覚, 日本獣医学会学術集会講演要旨集, 166th, 2023

マレック病ウイルスMeqタンパク質における挿入配列による病原性増強機構の解明
佐藤純平, 村田史郎, YEE Win Shwe, 瀬尾光里, もたい吉之介, 前川直也, 岡川朋弘, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 166th, 2023

新たに認められた欠損配列を持つマレック病ウイルスmeq遺伝子の性状解析
もたい吉之介, 村田史郎, 佐藤純平, 西明仁, 前川直也, 岡川朋弘, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 166th, 2023

特集：ワクチン戦略を考える：ワクチンの基本　　　　　　　　　　　　　　　
今内 覚, 臨床獣医, 11, 16, 21, Nov. 2022, [Invited], [Lead author, Last author, Corresponding author]
Japanese, Introduction scientific journal

牛伝染性リンパ腫ウイルス感染牛における分娩が及ぼす免疫抑制メカニズムの解析
富永みその, 今内覚, 佐治木大和, 岡川朋弘, 小原潤子, 似内厚之, 高橋博文, 窪田健太郎, 武田休史, 前川直也, 村田史郎, 大橋和彦, 北海道獣医師会雑誌, 66, 8, 2022

牛伝染性リンパ腫の病態発生機序解析を基盤とする新制御法の試み
今内 覚, 岡川朋弘, 前川直也, 村田史郎, 大橋和彦, 家畜衛生学雑誌, 47, 3, 147, 150, Dec. 2021, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

発酵哺乳飼料による子牛の腸炎防御効果の直接証明　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, JATAFFジャーナル, 9, 10, 21, 26, Oct. 2021, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

京都府の1酪農場における牛白血病ウイルス対先の乳生産性・繁殖性ならびに疾病発生に及ぼす効果　　　　　　　　　　　　　　　
北野菜奈, 吉田 陽, 今内 覚, 高橋俊彦, 家畜感染症学雑誌, 10, 3, 71, 77, Oct. 2021, [Peer-reviewed]
Japanese, Report scientific journal

子牛下痢症対策のアップデート：子牛下痢症への発酵代用乳の適応　　　　　　　　　　　　　　　
岡川朋弘, 今内 覚, 臨床獣医, 9, 34, 38, Sep. 2021, [Invited], [Last author, Corresponding author]
Japanese, Introduction scientific journal

技術ワイド：特集：哺育牛の飼養管理を見直す① 下痢の重症化を抑止し哺乳量も早期回復 乳酸菌添加した発酵代用乳による下痢対策　　　　　　　　　　　　　　　
今内 覚, DAIRYMAN, 71, 8, 44, 46, Aug. 2021, [Invited], [Lead author, Corresponding author]
Japanese, Report scientific journal

プロバイオティクスで子牛を下痢から守る 〜高品質で安全な発酵代用乳〜　　　　　　　　　　　　　　　
岡川 朋弘, 今内 覚, MPアグロジャーナル, 45, 7, 34, 37, Jul. 2021, [Invited], [Last author]
Japanese, Report scientific journal

臨床免疫を基盤とする産学協同応用研究の推進　　　　　　　　　　　　　　　
今内 覚, 臨床獣医, 6, 82, 84, Jun. 2021, [Invited], [Lead author, Corresponding author]
Japanese, Book review

Expression and functional analysis of swine PD-1 /PD-L1 pathway
OTGONTUYA Ganbaatar, 今内覚, 岡川朋弘, 野島裕太郎, 前川直也, 市川世識, 小林篤史, 芝原友幸, 柳川洋二郎, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021

ワクモ(Dermanyssus gallinae)の吸血に対する宿主免疫応答の解明
藤澤宗太郎, 村田史郎, 伊勢崎政美, 佐藤匠, 大石英司, 種子野章, 前川直也, 岡川朋弘, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021

犬の口腔内悪性黒色腫に対する抗Programmed death-Ligand 1抗体療法と放射線治療におけるアブスコパル効果の誘導解析
大脇稜, 出口辰弥, 細谷謙次, 今内覚, 前川直也, 立花由莉加, 中村基司, 金尚昊, 須永隆文, 奥村正裕, 北海道獣医師会雑誌, 65, 8, 2021

牛白血病の病態発生機序解析を基盤とする新制御法の試み
今内 覚, 岡川朋弘, 前川直也, 村田史郎, 大橋和彦, 畜産技術, 784, 36, 40, Sep. 2020, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

Immunotherapy〜Immune checkpoint inhibitor〜
今内 覚、岡川朋弘、前川直也、中島千絵、鈴木定彦、山本啓一、戸田幹洋、村田史郎、大橋和彦, Japan Veterinary Products Association Digest, 67, 2, 1, 10, Feb. 2020, [Invited], [Lead author, Corresponding author]
日本動物薬事協会, Japanese, Introduction scientific journal

分子標的薬-将来の展望 免疫チェックポイント阻害薬を用いた腫瘍免疫療法
前川直也, 今内覚, 岡川朋弘, 村田史郎, 大橋和彦, Veterinary Oncology, 7, 2, 2020

ネコCytotoxic T-lymphocyte antigen-4(CTLA-4)を標的とした新規免疫抑制剤の開発における基礎的検討
大塚拓海, 今内覚, 前川直也, 渡慧, 岡川朋弘, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 163rd, 2020

ワクモにおける定量的PCR法確立のための内在性コントロール遺伝子の探索
有泉拓馬, 村田史郎, 藤澤宗太郎, 伊勢崎政美, 前川直也, 岡川朋弘, 種子野章, 大石英司, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 163rd, 2020

牛白血病ウイルス感染症における免疫抑制受容体TIM-3の発現解析および機能解析
中村隼人, 今内覚, 岡川朋弘, 佐治木大和, 渡慧, 神谷可菜, 齋藤麻矢, 前川直也, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 163rd, 2020

ウシマイコプラズマ感染症における免疫疲弊化　　　　　　　　　　　　　　　
今内 覚, 後藤伸也, 岡川朋弘, 前川直也, 村田 史郎, 大橋和彦, 日本マイコプラズマ学会雑誌, 46, 31, 33, Dec. 2019, [Invited], [Lead author, Corresponding author]
Japanese, Report scientific journal

Tick factors associated pathogen transmission ~Tick immunosuppressants in saliva~
Satoru Konnal, Med. Entomol. Zool., 70, 4, 189, 197, Dec. 2019, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
マダニの唾液の役割と、唾液に含まれる免疫抑制因子を中心に取り上げ、同定され始めた病原体伝播に関わるマダニ因子について紹介した。ダニの唾液または唾液を産生する唾液腺を用いた研究から、マダニは宿主免疫を撹乱したり抑制したりすることが報告されており、唾液は直接的または間接的に病原体の伝播を補助・促進することも明らかとなっている。同定された免疫抑制因子のうちSalp15は宿主CD4+T細胞のCD4に結合し、IL-2産生の抑制によるCD4+T細胞の増殖および活性化を阻害する。さらに近年になって、マダニの吸血補助や維持に関わる因子の他に、ダニ体内において病原体の伝播や定着に関与する特異的な分子の存在が明らかとなってきた。, 日本衛生動物学会, Japanese, Introduction scientific journal

技術ワイド 徹底防除―ヨーネ病 病態発生のメカニズムと新規制御法　　　　　　　　　　　　　　　
Satoru Konnal, DAIRYMAN, 69, 10, 40, 41, Oct. 2019, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

免疫チェックポイント分子Programmed death‐ligand 1(PD‐L1)を標的とする抗体薬による免疫療法が奏功した肺転移のある口腔内悪性黒色腫の犬の1例
竹内寛人, 前川直也, 今内覚, 高木哲, 細谷謙次, 賀川由美子, 岡川朋弘, 鈴木定彦, 鈴木定彦, 山本啓一, 山本啓一, 村田史郎, 大橋和彦, 北海道獣医師会雑誌, 63, 8, 343, 343, 09 Aug. 2019
(公社)北海道獣医師会, Japanese

ロタウイルス実験感染子牛モデルを用いた3.5倍発酵代用乳の腸炎抑制効果の検証
茅先史, 今内覚, 久保田学, 岡川朋弘, 佐治木大和, 渡慧, 小原潤子, 前川直也, 村田史郎, 大橋和彦, 北海道獣医師会雑誌, 63, 8, 302, 302, 09 Aug. 2019
(公社)北海道獣医師会, Japanese

免疫学的解析を基盤とした動物用創薬研究　　　　　　　　　　　　　　　
Satoru Konnal, 臨床獣医, 37, 3, 37, 41, Mar. 2019, [Invited], [Lead author, Corresponding author]
(株)緑書房, Japanese, Introduction scientific journal

免疫チェックポイント阻害剤による犬の悪性腫瘍に対する新規治療法
高木哲, 前川直也, 今内覚, 日本獣医師会獣医学術学会年次大会講演要旨集, 2018, 2019

免疫チェックポイントを標的とした免疫療法　　　　　　　　　　　　　　　
Satoru Konnal, The Japanese Society for Animal Vaccine and Biomedical Research News Letter, 18, 2, 3, Dec. 2018, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

動物難治性疾病に対する創薬研究
今内 覚, 岡川朋弘, 前川直也, 中島千絵, 鈴木定彦, 山本啓一, 戸田幹洋, 村田史郎, 大橋和彦, MPアグロジャーナル, 35, 10, 22, 25, Oct. 2018, [Invited], [Lead author, Corresponding author]
MPアグロ, Japanese, Introduction scientific journal

ヨーネ病の病態発生機序の解析　　　　　　　　　　　　　　　
今内 覚, 永田礼子, 川治聡子, 森 康行, 畜産技術, 761, 2, 6, Oct. 2018, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

免疫チェックポイント分子を標的とした腫瘍免疫療法 抗PD-1/PD-L1抗体療法　　　　　　　　　　　　　　　
前川直也, 今内 覚, 村田史郎, 大橋和彦, 高木 哲, 獣医畜産新報, 71, 9, 659, 663, Sep. 2018, [Invited]
Japanese, Introduction scientific journal

牛白血病における新規病態進行評価マーカーの検索
渡慧, 今内覚, 佐治木大和, 岡川朋弘, 前川直也, 後藤伸也, 村田史郎, 大橋和彦, 北海道獣医師会雑誌, 62, 8, 282, 282, 24 Aug. 2018
(公社)北海道獣医師会, Japanese

マレック病ウイルスの病原性進化機構の解明―病原性試験や全ゲノム解析の試み
大橋和彦, 村田史郎, 町田柚香, 伊勢崎政美, 今内覚, 日本獣医学会学術集会講演要旨集, 161st, 216, 216, 21 Aug. 2018
(公社)日本獣医学会, Japanese

免疫抑制因子PD‐1およびPD‐L1を標的としたイヌ腫瘍に対する新規免疫療法の検討
前川直也, 今内覚, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 233, 233, 21 Aug. 2018
(公社)日本獣医学会, Japanese

動物難治性疾病の免疫学的解析を基盤とした新規制御法の開発
今内覚, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 202, 202, 21 Aug. 2018
(公社)日本獣医学会, Japanese

地方病性牛白血病の簡易鑑別診断基準の確立と若齢発症牛の実態調査
田中晶菜, 今内覚, 岡川朋弘, 西森朝美, 前川直也, 戸塚知恵, 千葉由純, 池田昌穂, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 353, 353, 21 Aug. 2018
(公社)日本獣医学会, Japanese

ヨーネ病におけるプロスタグランジンE₂(PGE₂)動態解析及びCOX‐2阻害剤の免疫活性化効果の検討
佐治木大和, 今内覚, 岡川朋弘, 西森朝美, 前川直也, 後藤伸也, 永田礼子, 川治聡子, 森康行, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 341, 341, 21 Aug. 2018
(公社)日本獣医学会, Japanese

ウシCytotoxic T‐lymphocyte antigen‐4(CTLA‐4)の機能解析および慢性感染症における発現解析
渡慧, 今内覚, 岡川朋弘, 前川直也, 後藤伸也, 佐治木大和, 村田史郎, 鈴木定彦, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 340, 340, 21 Aug. 2018
(公社)日本獣医学会, Japanese

肺転移のある悪性黒色腫罹患犬に対するPD‐L1を標的とした抗体薬の治療効果
前川直也, 今内覚, 高木哲, 細谷謙次, 賀川由美子, 岡川朋弘, 和泉雄介, 出口辰弥, 鈴木定彦, 山本啓一, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 429, 429, 21 Aug. 2018
(公社)日本獣医学会, Japanese

イヌCytotoxic T‐lymphocyte antigen‐4(CTLA‐4)を標的としたバイオ医薬品の開発に向けた基礎的検討
石原悠太郎, 今内覚, 岡川朋弘, 前川直也, 鈴木定彦, 大田寛, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 430, 430, 21 Aug. 2018
(公社)日本獣医学会, Japanese

Mycoplasma bovis感染症における免疫抑制機序の解析
後藤伸也, 今内覚, 岡川朋弘, 前川直也, 佐治木大和, 渡慧, 樋口豪紀, 小岩政照, 田島誉士, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 342, 342, 21 Aug. 2018
(公社)日本獣医学会, Japanese

地方病性牛白血病若齢発症牛におけるプロウイルス挿入部位の網羅的解析
岡川朋弘, 今内覚, 西森朝美, 田中晶菜, 前川直也, 戸塚知恵, 千葉由純, 池田昌穂, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 351, 351, 21 Aug. 2018
(公社)日本獣医学会, Japanese

ミャンマー連邦共和国における鶏の吸血性外部寄生虫の分布状況調査
竹原昌生, 村田史郎, 片倉賢, MYINT MYINT Hmoon, SHWE YEE Win, SAW Bawm, LAT LAT Htun, YE HTUT Aung, MAR MAR Win, 伊勢崎政美, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 161st, 375, 375, 21 Aug. 2018
(公社)日本獣医学会, Japanese

ワクモ(Dermanyssus gallinae)由来カテプシンL用タンパク質の性状解析とワクチン抗原としての評価
村田史郎, 伊勢崎政美, 谷口綾香, 北條巧, 種子野章, 酒井英史, 宇野有紀子, 矢吹卓也, 市居修, 伊東拓也, 今内覚, 大橋和彦, 衛生動物, 69, 2, 103, 103, Jun. 2018
日本衛生動物学会, Japanese

牛難治性疾病に対するバイオ医薬の創出　　　　　　　　　　　　　　　
今内 覚, JATAFFジャーナル, 6, 5, 29, 33, May 2018, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

獣医療における抗体医薬の現状
今内 覚, 村田史郎, 大橋和彦, 家畜診療, 65, 4, 233, 240, Apr. 2018, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病の最新知見とウイルス量を基盤とした対策について　　　　　　　　　　　　　　　
今内 覚, 獣医公衆衛生研究, 20, 2, 12, 17, Mar. 2018, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病の現状と対策について　　　　　　　　　　　　　　　
今内 覚, 北海道肉牛研究会報, 22, 1, 11, Mar. 2018, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

ネコpodoplaninに対するモノクローナル抗体の樹立とネコ扁平上皮がんにおけるpodoplanin発現解析
山田慎二, 板井俊介, 賀川由美子, 今内覚, 金子美華, 加藤幸成, 加藤幸成, 日本獣医学会学術集会講演要旨集, 161st, 2018

牛白血病発症牛由来細胞株に発現するアンチセンス鎖転写産物
三苫修也, 原彰浩, 目堅博久, 目堅博久, 今内覚, 岡川朋弘, 関口敏, 乗峰潤三, 乗峰潤三, 日本獣医学会学術集会講演要旨集, 161st, 2018

京都府の1酪農場におけるBLV対策とその効果について　　　　　　　　　　　　　　　
北野 菜奈, 吉田 陽, 今内 覚, 菊 佳男, 高橋 俊彦, 家畜感染症学会誌, 6, 4, 178, 178, Dec. 2017
家畜感染症学会誌編集委員会, Japanese

牛の免疫応答を利用した難治性疾病の新規制御法開発　　　　　　　　　　　　　　　
今内 覚, 家畜衛生学雑誌, 43, 3, 101, 103, Nov. 2017, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

感染ウイルス定量解析によるリスク評価を基盤とする牛白血病対策　　　　　　　　　　　　　　　
佐治木大和, 今内 覚, 中島隆二, 畜産の研究, 71, 9, 729, 739, Sep. 2017, [Invited], [Corresponding author]
Japanese, Introduction scientific journal

日本に分布するマレック病ウイルス野外株の病原性の検討
村田史郎, 種子野章, 酒井英史, 町田柚香, 松山あゆ美, 伊勢崎政美, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 405, 30 Aug. 2017
Japanese

マレック病ウイルス弱毒株を用いた新規抗ワクモワクチン開発に向けた基礎研究
青山珠里愛, 村田史郎, 伊勢崎政美, 種子野章, 酒井英史, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 405, 30 Aug. 2017
Japanese

牛マイコプラズマ感染症における免疫抑制機序の解明
後藤伸也, 今内覚, 岡川朋弘, 西森朝美, 前川直也, 樋口豪紀, 小岩政照, 田島誉士, 小原潤子, 加藤幸成, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 370, 370, 30 Aug. 2017
(公社)日本獣医学会, Japanese

Theileria parva感染症における免疫チェックポイント因子の動態解析
今内覚, 岡山朋弘, 山田慎二, SIMUUNZA Martin, CONNELLEY Timothy, MORRISON Ivan, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 338, 30 Aug. 2017
Japanese

PD‐L1を標的としたイヌ用キメラ抗体医薬の作製と悪性黒色腫および未分化肉腫罹患犬における抗腫瘍効果の検討
前川直也, 今内覚, 高木哲, 賀川由美子, 岡川朋弘, 西森朝美, 池渕良洋, 和泉雄介, 出口辰弥, 加藤幸成, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 460, 460, 30 Aug. 2017
(公社)日本獣医学会, Japanese

牛白血病ウイルス(BLV)感染症においてプロスタグランジンE₂(PGE₂)が及ぼす免疫抑制機序の解明
佐治木大和, 今内覚, 西森朝美, 岡川朋弘, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 370, 30 Aug. 2017
Japanese

BLV感染症をモデルとした抗ウシPD‐L1キメラ抗体の臨床応用試験
西森朝美, 今内覚, 岡川朋弘, 前川直也, 池渕良洋, 後藤伸也, 佐治木大和, 鈴木定彦, 小原潤子, 小笠原諭, 加藤幸成, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 370, 370, 30 Aug. 2017
(公社)日本獣医学会, Japanese

COX‐2阻害薬と抗PD‐L1抗体の併用によるイヌ腫瘍疾患の制御法の検討
浅野裕美恵, 今内覚, 岡川朋弘, 前川直也, 西森朝美, 後藤伸也, 高木哲, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 160th, 371, 371, 30 Aug. 2017
(公社)日本獣医学会, Japanese

牛白血病ウイルス(BLV)感染ハイリスク牛における初めての子宮内感染直接証明　　　　　　　　　　　　　　　
佐治木 大和, 今内 覚, 西森 朝美, 岡川 朋弘, 永野 昌志, 小原 潤子, 村田 史郎, 大橋 和彦, 北海道獣医師会雑誌, 61, 8, 280, 280, Aug. 2017
(公社)北海道獣医師会, Japanese

わが国におけるダニ媒介性細菌感染症研究の現状 新興ボレリア感染症　　　　　　　　　　　　　　　
川端 寛樹, 佐藤 梢, 熊谷 由美, 大西 真, 安藤 秀二, 福士 秀悦, 下島 昌幸, 西條 政孝, 阿戸 学, 長谷川 秀樹, 関塚 剛史, 黒田 誠, 高野 愛, 伊藤 幸枝, 古野 希和, 山野 公明, 伊東 拓也, 今内 覚, 李 景利, Taylor Kyle, 中尾 亮, 中尾 稔, 小山 幸次郎, 兼古 稔, 福島 和子, 齊藤 智也, 中嶋 健介, 榊原 佳子, 増沢 俊幸, 藤田 博己, 日本細菌学雑誌, 72, 1, 34, 34, Feb. 2017
日本細菌学会, Japanese

マダニ唾液由来因子の病原体伝播における役割
今内覚, 伊東拓也, 高野愛, 川端寛樹, 安藤秀二, 村田史郎, 大橋和彦, 日本細菌学雑誌(Web), 72, 1, 35(J‐STAGE), 2017, [Invited], [Lead author, Corresponding author]
Japanese

ウシCytotoxic T‐lymphocyte antigen‐4(CTLA‐4)の機能解析と特異抗体の樹立
渡慧, 今内覚, 前川直也, 岡川朋弘, 西森朝美, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 159th, 365, 365, 30 Aug. 2016
(公社)日本獣医学会, Japanese

家畜における免疫チェックポイント関連因子の遺伝子比較解析とウシ用抗体を用いた動物種横断的研究
野島裕太郎, 今内覚, 西森朝美, 前川直也, 岡川朋弘, 森康行, 賀川由美子, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 159th, 364, 364, 30 Aug. 2016
(公社)日本獣医学会, Japanese

シュルツェマダニ(Ixodes persulcatus)由来sialostatinL2の機能解析
越智晶絵, 今内覚, 伊東拓也, 川端寛樹, 高野愛, 安藤秀二, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 159th, 339, 30 Aug. 2016
Japanese

ウシ腫瘍壊死因子(TNF‐α)デコイレセプターによる新規抗炎症剤の開発
藤澤宗太郎, 今内覚, 岡川朋弘, 西森朝美, 前川直也, 村田史郎, 鈴木定彦, 大橋和彦, 日本獣医学会学術集会講演要旨集, 159th, 364, 364, 30 Aug. 2016
(公社)日本獣医学会, Japanese

マレック病ウイルス日本分離株の全ゲノム解析
村田史郎, 町田柚香, 伊勢崎政美, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 159th, 399, 30 Aug. 2016
Japanese

腫瘍血管遮断薬CA4Pの犬の血管内皮細胞への影響の検討　　　　　　　　　　　　　　　
和泉 雄介, 青島 圭佑, 星野 有希, 今内 覚, 高木 哲, 日本獣医学会学術集会講演要旨集, 159回, 451, 451, Aug. 2016
(公社)日本獣医学会, Japanese

B細胞性リンパ腫罹患犬における免疫チェックポイント分子発現解析
倉島千尋, 田川道人, 高木哲, 前川直也, 今内覚, 新坊弦也, 富張瑞樹, 松本高太郎, 猪熊壽, 川本恵子, 宮原和郎, 日本獣医学会学術集会講演要旨集, 159回, 462, 462, Aug. 2016
(公社)日本獣医学会, Japanese

抗ワクモ(Dermanyssus gallinae)ワクチン開発に向けたワクモ由来フェリチン2の性状解析
伊勢崎政美, 村田史郎, 酒井英史, 矢吹卓也, 種子野章, 市居修, 伊東拓也, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 159回, 399, 399, Aug. 2016
(公社)日本獣医学会, Japanese

牛白血病への対策を練る〜牛白血病について〜
今内 覚, 村田 史郎, 大橋 和彦, 養牛の友, 7, 484, 32, 36, Jul. 2016, [Invited], [Lead author, Corresponding author]
日本畜産振興会, Japanese, Introduction scientific journal

増加する牛白血病とその対策
今内 覚, 村田 史郎, 大橋 和彦, 農家の友, 68, 2, 108, 110, Feb. 2016, [Invited], [Lead author, Corresponding author]
北海道農業改良普及協会, Japanese, Introduction scientific journal

ウシ慢性感染症における免疫応答の解析と新規制御法への応用
今内 覚, 畜産技術, 2, 729, 2, 5, Feb. 2016, [Invited], [Lead author, Corresponding author]
畜産技術協会, Japanese, Introduction scientific journal

初乳の衛生管理について 〜健康な子牛を育てるために：牛白血病防除の観点から〜　　　　　　　　　　　　　　　
今内 覚, 乳房炎防除対策研究会誌, 22, 22., 21, 23, Feb. 2016, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

Programmed death-1 (PD-1)　　　　　　　　　　　　　　　
今内 覚, Veterinary Oncology, 3, 1, 12, 14, Jan. 2016, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病における免疫応答
今内 覚, 産業動物臨床医学雑誌, 6, 3, 130, 132, Nov. 2015, [Invited], [Lead author, Corresponding author]
日本家畜臨床学会 ; 2010-, Japanese, Introduction scientific journal

牛白血病の現状と対策の例　　　　　　　　　　　　　　　
今内 覚, 宮城県獣医師会会報, 68, 4, 187, 193, Oct. 2015, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病ウイルス感染症における疲弊化T細胞の動態とその再活性化
岡川朋弘, 今内覚, 西森朝美, 前川直也, 池渕良洋, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 324, 324, 30 Aug. 2015
(公社)日本獣医学会, Japanese

イヌ腫瘍浸潤リンパ球におけるPD‐1発現解析と免疫チェックポイント分子を標的とした新規腫瘍免疫療法の検討
前川直也, 今内覚, 池渕良洋, 岡川朋弘, 足立真実, 高木哲, 賀川由美子, 賀川由美子, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 405, 405, 30 Aug. 2015
(公社)日本獣医学会, Japanese

日本に分布するマレック病ウイルス野外株の分離
町田柚香, 村田史郎, 伊勢崎政美, 酒井英史, 種子野章, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 354, 30 Aug. 2015
Japanese

シュルツェマダニ(Ixodes persulcatus)唾液腺由来免疫抑制因子sL2の同定および機能解析
越智晶絵, 今内覚, 岡川朋弘, 西森朝美, 前川直也, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 301, 301, 30 Aug. 2015
(公社)日本獣医学会, Japanese

日本国内における牛白血病若齢発症牛の実態調査と表現型解析
西森朝美, 今内覚, 池渕良洋, 岡川朋弘, 中原綾子, 千葉由純, 戸塚知恵, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 336, 30 Aug. 2015
Japanese

ワクモ(Dermanyssus gallinae)由来カテプシンL様タンパク質の性状解析とワクチン抗原としての評価
北條巧, 谷口綾香, 村田史郎, 伊勢崎政美, 酒井英史, 矢吹卓也, 寺田晴菜, 種子野章, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 353, 30 Aug. 2015
Japanese

マレック病ウイルスmeq遺伝子に認められた欠損配列
村田史郎, 山本英次, 坂下奈津美, 松山あゆ美, 伊勢崎政美, 町田柚香, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 354, 30 Aug. 2015
Japanese

シュルツェマダニ(Ixodes persulcatus)由来Salp15因子によるライム病ボレリア伝播促進機能の解析
村瀬優介, 今内覚, 伊東拓也, 高野愛, 川端寛樹, 安藤秀二, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 158th, 301, 30 Aug. 2015
Japanese

牛の感染症の対策・防除を考える　　　　　　　　　　　　　　　
今内 覚, 臨床獣医, 33, 8, 11, 39, Aug. 2015, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

北海道におけるマダニ媒介性新興回帰熱病原体ボレリアの分布調査
川端寛樹, 川端寛樹, 今内覚, 高野愛, 中尾稔, 伊東拓也, 佐藤梢, 衛生動物, 66, 2, 43, 43, Jun. 2015
日本衛生動物学会, Japanese

牛白血病を出さない！尾鈴のBL対策　　　　　　　　　　　　　　　
今内 覚ら 他, 現代農業, 6, 312, 322, Jun. 2015, [Invited]
Japanese, Introduction other

牛白血病 最近の知見と対策について　　　　　　　　　　　　　　　
今内 覚, 動薬研究, 6, 71, 1, 11, Jun. 2015, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

マレック病ウイルス癌遺伝子(Meq)と病原性との関連
高木道浩, 村田史郎, 今内覚, 大橋和彦, 鶏病研究会報, 50, 4, 199, 205, 25 Feb. 2015
マレック病(MD)は鶏のウイルス感染症であり，家畜伝染病予防法で届出伝染病に指定されている。MDは翼麻痺や脚麻痺などの神経症状を示し，末梢神経の腫大，悪性リンパ腫形成(CD4+T細胞を標的とする)が見られ，死亡率は様々である。原因ウイルスであるMDウイルス(MDV)は直鎖状の二本鎖DNAウイルスでヘルペスウイルス科(Herpesviridae)アルファヘルペスウイルス亜科(Alphaherpesvirinae)，マルディウイルス(Mardivirus)に属する。これまではその病原性に基づきMDVは血清型1から3型(MDV1～3)に分類されていたが，最近，MDV1はGallid herpesvirus 2(GaHV-2)，MDV2はGallid herpesvirus 3(GaHV-3)およびMDV3はMeleagrid herpesvirus 1(MeHV-1)と新たに区分された。MDはワクチンによって効果的にコントロールできる疾病であり，ワクチンとしてすべての血清型があり，GaHV-2では弱毒株が用いられている。しかし，国内ではワクチンブレークによる発生，国外では強毒株の発生が見られている。また，ワクチンの作用機序およびMDVの腫瘍化機序も未だに解明されていない。ここではMDVの癌遺伝子と考えられているmeqについて述べると共に最近のMDV腫瘍化機序の知見について述べる。, 鶏病研究会, Japanese

アフリカのゴミ集積場における化学物質の汚染実態と生体への健康影響の評価:ナイロビのダンドラ地域における家畜の重金属汚染の例
中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, YARED YB, YABE John, 今内覚, 石塚真由美, 日本獣医学会学術集会講演要旨集, 157th, 533, 11 Aug. 2014
Japanese

牛白血病ウイルス感染牛におけるナチュラル・キラー細胞の動態および機能解析
中原綾子, 今内覚, 池渕良洋, 岡川朋弘, 西森朝美, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 157th, 388, 11 Aug. 2014
Japanese

ウシ制御性T細胞の機能解析および慢性感染症における動態解析
大平浩輔, 今内覚, 岡川朋弘, 西森朝美, 前川直也, 中原綾子, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 157th, 388, 388, 11 Aug. 2014
(公社)日本獣医学会, Japanese

イヌ免疫抑制因子PD‐1およびPD‐L1を標的とした新規腫瘍免疫療法の検討
前川直也, 今内覚, 池渕良洋, 岡川朋弘, 足立真実, 高木哲, 賀川由美子, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 157th, 483, 483, 11 Aug. 2014
(公社)日本獣医学会, Japanese

Direct PCR法を用いた全血からの牛白血病ウイルス迅速簡易診断法の検討
西森朝美, 今内覚, 池渕良洋, 岡川朋弘, 大平浩輔, 中原綾子, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 157th, 424, 11 Aug. 2014
Japanese

ケニアの大規模ゴミ集積場・ダンドラ地域における家畜の化学物質汚染の実態と住民の健康影響評価
中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, YOHANNES Yared B, 今内覚, 石塚真由美, J Toxicol Sci, 39, Supplement, S336, 187, Jul. 2014
ケニア共和国の首都ナイロビ市内の大規模ゴミ集積場・ダンドラ地域は、世界第二位の巨大・高密度スラム街であり、地域内の子供の半数はWHO基準（100 μg/kg）以上の血中Pb濃度であることが報告されているが、家畜の汚染や生体への曝露源に関する報告はない。本研究では、ダンドラおよび対照区として同国・ナクル地域に生息するヤギ、ヒツジ、ブタおよびウシの血中金属類（10元素）濃度およびPb安定同位体比をICP-MSにより、PCBsおよび有機塩素系農薬（OCPs）濃度をGC-MSとGC-ECDにより測定した。<br> その結果、全てのサンプルでCr, Mn, Ni, Cu, Zn, As, Mo, Agの高濃度蓄積は認められず、PCBsおよびOCPsは検出限界以下であった。Pb濃度はナクルに比べてダンドラで高い蓄積傾向を示し、ヤギおよびヒツジでは有意差が認められた。ブタの平均Pb濃度はダンドラ（2600 μg/kg, dry wt:dw）がナクル（73 μg/kg, dw）の約35倍の高値を示した。ダンドラのウシからは、ヘム合成に関与するアミノレブリン酸脱水酵素活性が低下するとされる値（100μg/kg）と同程度のPb濃度（91 μg/kg以上）が検出され、血液毒性等の中毒症状の蔓延が示唆された。Cd濃度の地域差および種差は認められなかったが、総じて高値（570±460 ng/kg, dw）を示し、ウシの摂食によるヒトのCd曝露が示唆された。Pb安定同位体比は地域および動物種により異なる傾向を示し、地域内に複数の曝露源があること、動物種により主要な曝露源が異なることが示唆された。<br> 本研究より、両地域に生息する家畜には高濃度のPb, Cdが蓄積し、特にPb汚染はダンドラで深刻なレベルであり、その曝露源が複数存在することが示唆された。家畜と生活環境を共にするヒトへの同様の汚染も強く示唆された。, 日本毒性学会, Japanese

アフリカのゴミ集積場における化学物質の汚染実態と生体への健康影響の評価:ナイロビのダンドラ地域における重金属汚染の例
中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, YOHANNES Yared B, 今内覚, 石塚真由美, 環境化学討論会要旨集(CD-ROM), 23rd, ROMBUNNO.3D-01, 14 May 2014
Japanese

我が国におけるマダニ媒介性新興回帰熱病原体ボレリアの分布調査
川端 寛樹, 今内 覚, 高野 愛, 中尾 稔, 伊東 拓也, 門馬 直太, 安藤 匡子, 高田 伸弘, 佐藤 梢, 大西 真, 衛生動物, 65, Suppl., 59, 59, Mar. 2014
日本衛生動物学会, Japanese

遡及調査により明らかとなった新興回帰熱の2症例
佐藤 梢, 高野 愛, 今内 覚, 中尾 稔, 伊東 拓也, 小山 幸次郎, 兼古 稔, 大西 真, 川端 寛樹, 衛生動物, 65, Suppl., 59, 59, Mar. 2014
日本衛生動物学会, Japanese

新興回帰熱Borrelia miyamotoi感染症に関する調査およびin vitro抗菌薬感受性
川端 寛樹, 高野 愛, 大西 真, 佐藤 梢, 今内 覚, 中尾 稔, 伊東 拓也, 小山 幸次郎, 兼古 稔, 日本細菌学雑誌, 69, 1, 148, 148, Feb. 2014
日本細菌学会, Japanese

Changes in peripheral leukocyte population and cytokine expression in dairy cows infected with bovine leukemia virus after oral administration of interferon-alpha
柿沼 清市, 大塚 浩通, 今内 覚, 家畜感染症学会誌 = The journal of farm animal in infectious disease, 3, 3, 77, 82, 2014
家畜感染症学会, Japanese

シュルツェマダニ(lxodes persulcatus)由来因子の宿主免疫抑制機能の解析
今内覚, 伊東拓也, 川端寛樹, 高野愛, 安藤秀二, 村田史郎, 大橋和彦, 大原綜合病院年報, 53, 65, 15 Dec. 2013, [Lead author, Corresponding author]
Japanese

免疫抑制受容体PD-1のリガンドPD-L2の機能的特徴と臨床応用研究　　　　　　　　　　　　　　　
西森朝美, 今内覚, 池渕良洋, 岡川朋弘, 村田史郎, 大橋和彦, 動物ワクチン-バイオ医薬品研究会ニュースレター, 8, 18, 19, Dec. 2013, [Invited], [Corresponding author]
Japanese, Introduction scientific journal

牛白血病ウイルスの感染予防対策　　　　　　　　　　　　　　　
今内 覚, 村田 史郎, 大橋 和彦, DAIRYMAN, 63, 9, 42, 43, Sep. 2013, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

イヌ免疫抑制因子PD‐1およびPD‐L1の同定と新規免疫療法樹立への基礎研究
前川直也, 今内覚, 池渕良洋, 岡川朋弘, 高木哲, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 156th, 257, 257, 30 Aug. 2013
(公社)日本獣医学会, Japanese

牛白血病ウイルス感染牛由来IgM^high B細胞とIgM^low B細胞のウイルス抗原発現解析及び性状解析
池渕良洋, 今内覚, 岡川朋弘, 西森朝美, 中原綾子, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 156th, 271, 30 Aug. 2013
Japanese

マレック病腫瘍病変形成における免疫抑制因子発現機序の検討
松山あゆ美, 村田史郎, 伊勢崎政美, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 156th, 257, 30 Aug. 2013
Japanese

シュルツェマダニ由来免疫抑制因子の機能解析
豊間根耕地, 今内覚, 伊東拓也, 川端寛樹, 高野愛, 安藤秀二, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 156th, 235, 30 Aug. 2013
Japanese

全血培養法によるエンドトキシン活性測定の有用性の検討
今村彩貴, 中溝万里, 川西路子, 池渕良洋, 目堅博久, 村田史郎, 今内覚, 大橋和彦, 中島奈緒, 山本欣也, 内山万利小, 平野文哉, 永井英貴, 木島まゆみ, 日本獣医学会学術集会講演要旨集, 156th, 260, 30 Aug. 2013
Japanese

ウシ免疫抑制因子PD‐L1およびPD‐L2の組換えタンパク質作製と免疫機能解析
西森朝美, 今内覚, 池渕良洋, 岡川朋弘, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 156th, 257, 30 Aug. 2013
Japanese

牛白血病発生農場におけるサシバエ種の同定とウイルスの検出
今内覚, 豊間根耕地, 小池菜々子, 伊東拓也, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 156th, 231, 30 Aug. 2013
Japanese

国内に潜在すると考えられる新興回帰熱に関する疫学調査研究
高野愛, 川端寛樹, 大久保(佐藤)梢, 中尾稔, 伊東拓也, TAYLOR Kyle, 李景利, 坪田敏男, 今内覚, 吉村英紘, 豊間根耕地, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 156回, 304, 304, Aug. 2013
(公社)日本獣医学会, Japanese

シュルツェマダニにおける病原体伝播関連因子の同定.
今内覚, 川端寛樹, 伊東拓也, 高野愛, 安藤秀二, 村田史郎, 大橋和彦, 衛生動物, 64, 2, 112, 15 Jun. 2013, [Lead author, Corresponding author]
Japanese

回帰熱病原体Borrelia miyamotoiの検出法の確立・検証,およびIxodes属ダニからの病原体検出への応用.
高野愛, 佐藤梢, 中尾稔, 伊東拓也, TAYLOR Kyle, 今内覚, 坪田敏男, 高田伸弘, 川端寛樹, 衛生動物, 64, 2, 113, 113, Jun. 2013
日本衛生動物学会, Japanese

スーラ病の分子疫学調査および野外分離株を用いた比較病原性解析と病態発生機序の解明
目堅博久, 今内覚, MINGALA Claro N, 井上昇, 村田史郎, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 155th, 81, 04 Mar. 2013
Japanese

RNA用試料抽出・保存用濾紙のRNAウイルスPCR検出への利用
松山智慧麿, 遠藤大二, 大橋和彦, 今内覚, 村田史郎, 有川二郎, 吉松組子, 水谷哲也, 林正信, 日本獣医学会学術集会講演要旨集, 155th, 229, 04 Mar. 2013
Japanese

Recent Findings in Bovine Infection Immunity
KONNAI Satoru, Journal of the Japan Veterinary Medical Association, 66, 3, 171, 179, Mar. 2013, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
病原微生物の侵入に対して宿主は免疫によって対抗する。牛においても病原微生物に対する感染防御機構は，人やマウスと同様である。すなわち，食細胞を中心とした自然免疫とリンパ球による獲得免疫の協調作用により病原微生物に対抗する。自然免疫系における食細胞による病原体の認識機構は，微生物間で共通の分子構造を抗原非特異的に認識するのに対し，獲得免疫系におけるリンパ球は，受容体の遺伝子再構成により多彩なレパートリーを形成し，微細な抗原構造を特異的に認識する機構を備えている。このようなリンパ球の一部は，免疫を記憶した亜集団へ分化し，病原体の生体内への再侵入時に強い免疫応答を誘導する。このように宿主が備え持つ感染免疫は，非特異的防御のみならず抗原特異性や免疫記憶を有しており，病原体排除に重要な役割を担う。しかし，種々の感染症の中には，予防や治療が困難な感染症がまだ数多く存在する。これらの原因の一つは，病原微生物が免疫回避や攪乱を引き起こし病態が進行することにある。このような感染症に対し有効な治療薬やワクチンを開発するためには，病原体と宿主の相互作用を解明することが重要である。本総説では，どのような異常な免疫応答によって病態が形成され死に至るのか，またどのような免疫回避機構で持続感染が成立し病態を発揮するのか，国内外の感染免疫に関する研究動向を簡単に解説するとともに，これらに関する最近の牛における知見について紹介したい。, Japan Veterinary Medical Association, Japanese, Introduction scientific journal

シュルツェマダニ由来因子の病原体伝播における機能解析
今内覚, 川端寛樹, 伊東拓也, 高野愛, 安藤秀二, 村田史郎, 大橋和彦, 大原綜合病院年報, 52, 105, 106, 15 Dec. 2012, [Lead author, Corresponding author]
Japanese

カメキララマダニより検出された新種回帰熱ボレリアの経ステージ感染様式~ボレリア属細菌の進化の軌跡を探る~
高野愛, 杉森千恵子, 藤田博己, 角坂照貴, TAYLOR Kyle, 坪田敏男, 今内覚, 田島朋子, 佐藤梢, 渡邉治雄, 大西真, 川端寛樹, 大原綜合病院年報, 52, 106, 107, Dec. 2012
(一財)大原記念財団, Japanese

感染症と免疫の基礎 臨床現場で役立つ免疫の知識　　　　　　　　　　　　　　　
大橋 和彦, 村田 史郎, 今内 覚, 臨床獣医, 11, 50, 52, Nov. 2012, [Invited]
Japanese, Introduction scientific journal

感染症と免疫の基礎 細胞性免疫　　　　　　　　　　　　　　　
今内 覚, 村田 史郎, 大橋 和彦, 臨床獣医, 10, 42, 44, Oct. 2012, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

感染症と免疫の基礎 液性免疫　　　　　　　　　　　　　　　
村田 史郎, 今内 覚, 大橋 和彦, 臨床獣医, 9, 60, 62, Sep. 2012, [Invited]
Japanese, Introduction scientific journal

ワクモDermanyssus gallinae由来発現遺伝子の網羅的解析
伊勢崎政美, 村田史郎, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 154th, 203, 31 Aug. 2012
Japanese

フィリピン共和国におけるTrypanosoma evansi野外流行株の分離および比較病原性解析
今内覚, 目堅博久, CLARO Mingala, NANCY Abes, ALAN Dargantes, 井上昇, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 154th, 213, 31 Aug. 2012
Japanese

マレック病ウイルスワクチン接種鶏における免疫抑制因子PD‐1およびPD‐L1の発現解析
高崎紗蘭, 村田史郎, 松山あゆ美, 伊勢崎政美, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 154th, 234, 31 Aug. 2012
Japanese

牛白血病ウイルス感染牛における免疫抑制因子Tim‐3/Gal‐9の発現解析および機能解析
岡川朋弘, 今内覚, 池渕良洋, 鈴木紗織, 寸田祐嗣, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 154th, 233, 31 Aug. 2012
Japanese

シュルツェマダニ(Ixodes persulcatus)唾液腺由来免疫抑制因子sL2の同定および発現解析
高田春奈, 今内覚, NAFTALY Githaka, 伊勢崎政美, 伊東拓也, 安藤秀二, 川端寛樹, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 154th, 204, 31 Aug. 2012
Japanese

ウシの免疫抑制受容体PD‐1に対するモノクローナル抗体の作製および免疫活性化能の検討
池渕良洋, 今内覚, 岡川朋弘, 横山和正, 中島千絵, 鈴木定彦, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 154th, 233, 31 Aug. 2012
Japanese

マレック病感染鶏由来腫瘍病変部における免疫抑制因子PD‐1/PD‐L1発現の形態学的解析
村田史郎, 松山あゆ美, 伊勢崎政美, 高崎紗蘭, 市居修, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 154回, 234, 234, Aug. 2012
(公社)日本獣医学会, Japanese

カメキララマダニより検出された回帰熱群ボレリアの経ステージ感染様式
高野愛, 杉森千恵子, 藤田博己, 角坂照貴, TAYLOR Kyle, 坪田敏男, 今内覚, 田島朋子, 佐藤梢, 渡邉治雄, 大西真, 川端寛樹, 日本獣医学会学術集会講演要旨集, 154回, 270, 270, Aug. 2012
(公社)日本獣医学会, Japanese

感染症と免疫の基礎 免疫とは何か　　　　　　　　　　　　　　　
大橋 和彦, 村田史郎, 今内 覚, 臨床獣医, 8, 60, 62, Aug. 2012, [Invited]
Japanese, Introduction scientific journal

増加している牛白血病 〜北海道での現状と対策について〜　　　　　　　　　　　　　　　
今内 覚, 村田 史郎, 大橋 和彦, 北海道獣医師会雑誌., 56, 1, 7, Jul. 2012, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

ウシ難治性疾病に対する多機能型新規治療法の開発　　　　　　　　　　　　　　　
岡川朋弘, 今内覚, 池渕良洋, 鈴木紗織, 村田史郎, 大橋和彦, 動物ワクチン-バイオ医薬品研究会ニュースレター, 5, 21, 23, Jun. 2012, [Invited], [Corresponding author]
Japanese, Introduction scientific journal

小誌連載「内科実習」各疾患の群管理・牛白血病対策　　　　　　　　　　　　　　　
今内 覚, 臨床獣医, 6, 44, 49, Jun. 2012, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病ウイルスに対する大規模牛群内での伝播防止コントロール　　　　　　　　　　　　　　　
今内 覚, 酪農ジャーナル, 4, 15, 18, Apr. 2012, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

地方病性牛白血病腫瘍組織における免疫抑制因子PD‐L1の発現とその役割
寸田祐嗣, 竹林賢作, 今内覚, 池渕良洋, 大橋和彦, 落合謙爾, 梅村孝司, 日本獣医学会学術集会講演要旨集, 153rd, 203, 203, 01 Mar. 2012
(公社)日本獣医学会, Japanese

A comparative analysis of cytokine profile of Theileria parva infection in Bovidae　　　　　　　　　　　　　　　
Okagawa T, Konnai S, Githaka N, Mekata H, Suzuki S, Kariuki E, Skilton R, Ishizuka M, Murata S, Ohashi K, Japanese Journal of Veterinary Parasitology, 10, 1-2, 37, Mar. 2012, [Corresponding author]
Japanese, Report scientific journal

Babesia ovata感染宿主有核細胞の培養系産物を抗原としたELISAによる抗体検出系
児玉道, 荻原喜久美, 納谷裕子, 村田史郎, 今内覚, 大橋和彦, 岸川正剛, 日本獣医学会学術集会講演要旨集, 153rd, 223, 01 Mar. 2012
Japanese

牛難治性疾病に対する免疫抑制因子を標的とした治療型ワクチンの開発
今内覚, 池渕良洋, 岡川朋弘, 鈴木紗織, 白井達哉, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 153rd, 117, 01 Mar. 2012
Japanese

牛白血病ウイルス感染牛における免疫抑制因子PD‐1/PL‐L1の発現解析および機能解析
池渕良洋, 今内覚, 村田史郎, 大橋和彦, 日本獣医師会獣医学術学会年次大会講演要旨集, 2011, 45, 20 Jan. 2012
Japanese

ウシTim-3の発現・機能解析と新規疾病制御法への応用展開　　　　　　　　　　　　　　　
岡川朋弘, 今内覚, 池渕良洋, 鈴木紗織, 村田史郎, 大橋和彦, 動物ワクチン-バイオ医薬品研究会ニュースレター, 4, 27, 29, Dec. 2011, [Invited], [Corresponding author]
Japanese, Introduction scientific journal

牛白血病の感染予防対策 最終回 4 ある農場における清浄化の取り組み事例
今内覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairy Jpn, 56, 15, 63, 65, 01 Dec. 2011, [Invited], [Lead author, Corresponding author]
Japanese

牛白血病の感染予防対策 —診療・生産現場からの声に対して—　　　　　　　　　　　　　　　
今内 覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairy Japan, 12, 62, 64, Dec. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病の感染予防対策 3 牛白血病に対する風評被害と間違った知識とは?
今内覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairy Jpn, 56, 14, 38, 40, 01 Nov. 2011, [Invited], [Lead author, Corresponding author]
Japanese

牛白血病の感染予防対策 牛白血病に対する風評被害と間違った知識とは？　　　　　　　　　　　　　　　
今内 覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairy Japan, 11, 57, 58, Nov. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病の感染予防対策 —今からできる予防策とは？—　　　　　　　　　　　　　　　
今内 覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairy Japan, 10, 34, 36, Oct. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

現場で活かす酪農技術 牛白血病の感染予防対策 1 牛白血病はなぜ増えたのか?
今内覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairy Jpn, 56, 11, 53, 55, 01 Sep. 2011, [Invited], [Lead author, Corresponding author]
Japanese

牛白血病の感染予防対策 —牛白血病はなぜ増えたのか？—　　　　　　　　　　　　　　　
今内 覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairy Japan, 9, 37, 39, Sep. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

シュルツェマダニ(Ixodes persulcatus)唾液腺由来免疫抑制因子の同定および発現解析
丹羽彩乃, 今内覚, GITHAKA Naftary, 伊東拓也, 川端寛樹, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 152nd, 207, 31 Aug. 2011
Japanese

マレック病ウイルス強毒株由来Meqおよび弱毒株由来L‐Meqによるbcl‐2遺伝子発現調節の比較
村田史郎, 伊勢崎政美, 松山あゆ美, 高崎紗蘭, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 152nd, 241, 31 Aug. 2011
Japanese

ウシ免疫抑制受容体Tim‐3の同定と牛白血病ウイルス感染牛における発現解析
岡川朋弘, 今内覚, 池渕良洋, 鈴木紗織, 寸田祐嗣, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 152nd, 224, 31 Aug. 2011
Japanese

牛白血病ウイルス感染牛における制御性T細胞の動態解析
鈴木紗織, 今内覚, 池渕良洋, 岡川朋弘, 寸田祐嗣, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 152nd, 224, 31 Aug. 2011
Japanese

トリパノソーマ原虫感染において制御性樹状細胞は炎症反応を抑制して病態の改善に関与する
目堅博久, 今内覚, 井上昇, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 152nd, 205, 31 Aug. 2011
Japanese

Theileria parva原虫感染牛属のサイトカイン発現比較解析
今内覚, 岡川朋弘, NAFTALY Githaka, 目堅博久, 鈴木紗織, EDWARD Kariuki, ROBERT Skilton, 石塚真由美, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 152nd, 204, 31 Aug. 2011
Japanese

ボレリア属細菌のマダニ体内動態と進化に関する研究.
高野愛, 川端寛樹, 藤田博己, 角坂照貴, 今内覚, 田島朋子, 渡邉治雄, 大西真, 衛生動物, 62, 2, 133, 15 Jun. 2011
Japanese

シュルツェマダニ(Ixodes persulcatus)におけるライム病ボレリア伝播関連因子の同定.
今内覚, 村瀬優介, 伊東拓也, 高野愛, 川端寛樹, 村田史郎, 大橋和彦, 衛生動物, 62, 2, 133, 15 Jun. 2011, [Lead author, Corresponding author]
Japanese

バイオセキュリティを見直す 4)感染牛を把握し,伝播リスクを抑える ウイルスを原因とする牛白血病の防疫
今内覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, Dairyman, 61, 6, 30, 31, 01 Jun. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

マダニ媒介性のライム病病原体Borrelia gariniiの維持伝播サイクルに関する研究　　　　　　　　　　　　　　　
川端 寛樹, 高野 愛, 中尾 稔, 増沢 俊幸, 高田 伸弘, 矢野 泰弘, 石畝 史, 藤田 博己, 伊東 拓也, 及川 陽三郎, 川森 文彦, 熊谷 邦彦, 三上 稔之, 花岡 希, 安藤 秀二, 本田 尚子, Taylor Kyle, 坪田 敏男, 今内 覚, 渡邉 治雄, 大西 真, 衛生動物, 62, Suppl., 69, 69, Apr. 2011
日本衛生動物学会, English

牛の難治性疾病に対する多機能型ワクチン戦略
今内覚, 池渕良洋, 寸田祐嗣, 小沼操, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 151st, 120, 01 Mar. 2011
Japanese

牛末梢血よりTheileria orientalisとBabesia ovataとの混合感染有核細胞培養系の樹立
児玉道, 荻原喜久美, 納屋裕子, 久保正法, 榊原伸一, 村田史郎, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 151st, 217, 01 Mar. 2011
Japanese

ベクターコントロールによる東海岸熱新規防除法の探索
山田慎二, 今内覚, 今村彩貴, SIMMUNZA Martin, NANBOTA Andrew, 小沼操, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 151st, 79, 01 Mar. 2011
Japanese

牛白血病の感染防御対策　　　　　　　　　　　　　　　
今内 覚, 田島誉士, 小沼 操, 村田史郎, 大橋和彦, 北海道しゃくなげ会会報, 50, 10, 12, Feb. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

国内の牛白血病の現状と今後の対策について　　　　　　　　　　　　　　　
今内 覚, 田島 誉士, 村田 史郎, 大橋 和彦, 牛臨床寄生虫研究会誌, 2, 2, 3, Feb. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

ワンヘルス理念に基づく動物由来感染症制御に関する研究 国内生態系におけるライム病ボレリアの維持伝播経路に関する研究
川端寛樹, 石ぐろ史, 藤田博己, 高田伸弘, 矢野泰弘, 増澤俊幸, 中尾稔, 伊東拓也, 及川陽三郎, TAYLOR Kyle, 坪田敏男, 今内覚, 川森文彦, 三上稔之, 熊谷邦彦, 高野愛, 安藤秀二, 花岡希, 本田尚子, 渡邉治雄, 大西真, ワンヘルス理念に基づく動物由来感染症制御に関する研究 平成22年度 総括・分担研究報告書, 17, 20, 2011
Japanese

獣医学における保全医学の展開―生物多様性と野生動物感染症―マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布
大橋和彦, 松原綾子, 村田史郎, 今内覚, 獣医畜産新報, 64, 1074, 21, 26, 01 Jan. 2011
文永堂出版, Japanese

牛白血病ワクチン開発への展望　　　　　　　　　　　　　　　
今内 覚, 家畜衛生学雑誌, 37, 81, 84, Jan. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

酪農現場における牛白血病対策の現状と課題　　　　　　　　　　　　　　　
今内 覚, 臨床獣医, 29, 14, 18, Jan. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

牛白血病の正体と予防を考察する　　　　　　　　　　　　　　　
今内 覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, HOLSTEIN MAGAZINE, 505, 4, 7, Jan. 2011, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

難治性慢性疾病における免疫疲弊化機序の解明と新規治療法への応用―獣医医療への応用にむけて―
池渕良洋, 今内覚, 寸田祐輔, 小沼操, 村田史郎, 大橋和彦, 日生研たより, 56, 6, 77, 81, 01 Nov. 2010, [Corresponding author]
Japanese

ザンビア共和国をモデルとした微量元素汚染:ウシにおける微量元素濃度とメタロチオネインおよびサイトカインmRNA発現量
室谷太郎, 池中良徳, 中山翔太, YABE John, MUZANDU Kaampwe, CHOONGO Kennedy, 今内覚, 梅村孝司, 石塚真由美, 日本獣医学会学術集会講演要旨集, 150th, 323, 01 Sep. 2010
Japanese

フィリピン共和国で分離したTrypanosoma evansiの病原性比較解析
目堅博久, 今内覚, MINGALA Claro, DARGANTES Alan, 井上昇, 小沼操, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 191, 01 Sep. 2010
Japanese

シュルツェマダニ(Ixodes persulcatus)由来Salp15様因子の性状解析
村瀬優介, 今内覚, 伊藤拓也, 高野愛, 川端寛樹, 小沼操, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 193, 01 Sep. 2010
Japanese

アフリカスイギュウ(Syncerus caffer)のTh1,Th2および炎症性サイトカイン遺伝子の同定
岡川朋弘, 鈴木紗織, 今内覚, GITHAKA Naftali, KARIUKI Edward, GAKUYA Francis, ESTHER Kanduma, 石塚真由美, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 212, 01 Sep. 2010
Japanese

マレック病ウイルス感染鶏における免疫抑制受容体PD‐1とそのリガンドPD‐L1の発現解析
河東あゆ美, 村田史郎, 加納里佳, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 214, 01 Sep. 2010
Japanese

牛白血病ウイルス感染牛における免疫抑制因子PD‐1/PD‐L1の発現解析と機能解析
池渕良洋, 今内覚, 白井達哉, 寸田祐嗣, 小沼操, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 214, 01 Sep. 2010
Japanese

シュルツェマダニ(Ixodes persulcatus)由来Salp16の宿主免疫抑機構解析
今内覚, 肥田野新, 村瀬優介, 伊東拓也, 川端寛樹, 樋口豪起, 小沼操, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 193, 01 Sep. 2010
Japanese

ウシ免疫抑制因子LAG‐3の同定と牛白血病ウイルス感染牛における発現解析
白井達哉, 今内覚, 池渕良洋, 寸田祐嗣, 小沼操, 村田史郎, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 214, 01 Sep. 2010
Japanese

ワクモDermanyssus gallinaeに対する新規防除法開発のためのトランスクリプトーム解析
伊勢崎政美, 村田史郎, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 193, 01 Sep. 2010
Japanese

マガンおよび鶏におけるinterferon‐γ遺伝子プロモーター活性の比較解析
松原綾子, 村田史郎, 加納里佳, 橋口知幸, 伊勢崎政美, 河東あゆ美, 小沼操, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 150th, 215, 01 Sep. 2010
Japanese

メガファームにおける取り組み紹介―牛白血病ウイルス感染伝播阻止トライアルの実例―
今内覚, 安富一郎, 田島誉士, 村田史郎, 大橋和彦, 北海道獣医師会雑誌, 54, 8, 378, 10 Aug. 2010, [Lead author, Corresponding author]
Japanese

ザンビア共和国をモデルとした重金属類汚染:家畜における重金属類濃度とバイオマーカーmRNA発現量
室谷太郎, 中山翔太, 池中良徳, YABE John, MUZANDU Kaampwe, CHOONGO Kennedy, 今内覚, 梅村孝司, 石塚真由美, 環境化学討論会講演要旨集, 19th, 816, 817, 20 Jun. 2010
Japanese

難治性慢性疾病における免疫疲弊化機序の解明と新規治療法への応用〜獣医医療への応用に向けて〜　　　　　　　　　　　　　　　
池渕良洋, 今内 覚, 寸田祐嗣, 小沼 操, 村田史郎, 大橋和彦, NIBS Letter, 56, 6, 7, 11, Jun. 2010, [Invited], [Corresponding author]
Japanese, Introduction scientific journal

増加傾向にある牛白血病の現状と対策 〜診療現場からの声に対して〜
今内 覚, 田島 誉士, 小沼 操, 村田 史郎, 大橋 和彦, 産業動物臨床医学雑誌, 1, 2, 110, 114, Jun. 2010, [Invited], [Lead author, Corresponding author]
Japanese Society of Large Animal Clinics / Large Animal Clinic Research Association, Japanese, Introduction scientific journal

増加傾向にある牛白血病の現状と対策 〜診療・生産現場からの声に対して〜
今内 覚, 田島 誉士, 小沼 操, 大橋 和彦, 家畜診療, 57, 4, 201, 207, Apr. 2010, [Invited], [Lead author, Corresponding author]
全国農業共済協会, Japanese, Introduction scientific journal

ダニ媒介性感染症に対する抗ダニワクチンの開発　　　　　　　　　　　　　　　
今内 覚, 家畜衛生学雑誌, 36, 73, 75, Apr. 2010, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布
大橋和彦, 松原綾子, 村田史郎, 今内覚, 日本獣医学会学術集会講演要旨集, 149th, 198, 2010
Japanese

The detection and characterization of the meq variants of Marek's diseases virus　　　　　　　　　　　　　　　
Ohashi K, Murata S, Okada T, Konnai S, Animal Viruses, 16, 117, 132, Jan. 2010, [Peer-reviewed], [Invited]
Japanese, Introduction scientific journal

牛白血病ウイルス感染症の現状と対策〜清浄化への道、まずは新たな感染を防ぐことから〜　　　　　　　　　　　　　　　
今内 覚, 北海道獣医師会雑誌., 53, 529, 534, Sep. 2009, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

シュルツェマダニにおけるライム病ボレリア伝播関連遺伝子の同定
今内覚, 村瀬優介, 山田慎二, 肥田野新, 伊東拓也, 川端寛樹, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 148th, 2009

Theileria parva感染牛に認められた炎症性サイトカインストーム
山田慎二, 今内覚, 今村彩貴, SIMUUNZA Martin, CHEMBENSOFU Mwelwa, AMOS Chota, NAMBOTA Andrew, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 146th, 170, 05 Sep. 2008
Japanese

マレック病ウイルスmeq遺伝子の多型に伴うMeqタンパクの転写活性化能の変化
橋口知幸, 村田史郎, 岡田宰, 加納里佳, 今内覚, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 146th, 189, 05 Sep. 2008
Japanese

弱毒および強毒株マレック病ウイルス感染鶏における免疫応答の比較
加納里佳, 橋口知幸, 岡田宰, 村田史郎, 今内覚, 小沼操, 大橋和彦, 日本ウイルス学会北海道支部夏季シンポジウム講演抄録, 42nd, 14, 2008
Japanese

帯広近郊,ロシアならびにカムチャッカにおける野生水禽類のToxoplasma gondii抗体保有状況
村尾岳洋, 加納里佳, 村田史郎, 今内覚, 大橋和彦, 小沼操, 前田龍一郎, 小俣吉孝, 日本野生動物医学会大会・講演要旨集, 13th, 88, 06 Sep. 2007
Japanese

コイタマダニにおけるオスダニ由来因子の同定と発現解析
山田慎二, 今村彩貴, 今内覚, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 144th, 69, 27 Aug. 2007
Japanese

コイタマダニ由来新規シスタチン遺伝子のクローニングと性状解析
今村彩貴, 山田慎二, 今内覚, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 144th, 69, 27 Aug. 2007
Japanese

シュルツェマダニ由来defensin遺伝子のクローニングおよび発現解析
斉藤洋一, 今内覚, 西門秀人, 山田慎二, 今村彩貴, 森亜紀奈, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 144th, 67, 27 Aug. 2007
Japanese

マレック病ウイルス弱毒株由来L‐Meqと強毒株由来Meqの機能における比較解析
村田史郎, 岡田宰, 加納里佳, 小沼操, 今内覚, 大橋和彦, 日本獣医学会学術集会講演要旨集, 144th, 83, 27 Aug. 2007
Japanese

弱毒および強毒マレック病ウイルス感染鶏における免疫応答の比較
加納里佳, 村田史郎, 岡田宰, 今内覚, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 144th, 77, 27 Aug. 2007
Japanese

帯広近郊,ロシアならびにカムチャッカにおける野生カモ類のトキソプラズマ抗体保有状況
村尾岳洋, 加納里佳, 村田史郎, 今内覚, 大橋和彦, 小沼操, 前田龍一郎, 小俣吉孝, 日本獣医学会学術集会講演要旨集, 144th, 65, 27 Aug. 2007
Japanese

マレック病ウイルス発癌遺伝子meqのスプライス産物Δmeqの発現・性状解析
岡田宰, 村田史郎, 加納里佳, 高木道浩, 今内覚, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 144th, 83, 27 Aug. 2007
Japanese

Interferon-gamma expression associated with suppression of bovine leukemia virus at the early phase of infection in sheep
Tatsufumi Usui, Satoru Konnai, Kazuhiko Ohashi, Misao Onuma, AIDS RESEARCH AND HUMAN RETROVIRUSES, 23, 4, 609, 609, Apr. 2007
English, Summary international conference

Tumor necrosis factor-alpha genetic polymorphism may contribute to progression of bovine leukemia virus infection
Satoru Konnai, Tatsufumi Usui, Manabu Ikeda, Junko Kohara, Kosuke Okada, Kazuhiko Ohashi, Misao Onuma, AIDS RESEARCH AND HUMAN RETROVIRUSES, 23, 4, 608, 609, Apr. 2007
English, Summary international conference

犬腫瘍細胞溶解産物を感作した樹状細胞ワクチンの有用性に関する基礎的研究
横川 美穂, 今内 覚, 星野 有希, 高木 哲, 奥村 正裕, 藤永 徹, 日本獣医学会学術集会講演要旨集, 143回, 211, 211, Mar. 2007
(公社)日本獣医学会, Japanese

Theileria parva感染Rhipicephalus appendiculatusによる宿主への実験的伝播
今内覚, 山田慎二, 今村彩貴, MARTIN Simuunza, MWELWA Chembensofu, ANDREW Nambota, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 142nd, 67, 31 Aug. 2006
Japanese

マレック病由来腫瘍細胞株におけるMeqバリアント(変異体)の機能解析
岡田宰, 高木道浩, 村田史郎, 加納里佳, 林裕子, 今内覚, 小沼操, 大橋和彦, 日本獣医学会学術集会講演要旨集, 142nd, 80, 31 Aug. 2006
Japanese

マレック病ウイルスの強毒化とmeq遺伝子の多型との関連
村田史郎, 岡田宰, 加納里佳, 林裕子, 今内覚, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 142nd, 80, 31 Aug. 2006
Japanese

コイタマダニ由来セルピンの抗ダニカクテルワクチン効果
今村彩貴, BONIFACE Mamangala, 今内覚, 中島千絵, MWASE Tembo, 田島朋子, 安田準, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 84, 31 Aug. 2005
Japanese

コイタマダニにおける精巣由来オス因子の探索
伊藤裕子, 今内覚, 今村彩貴, 中島千絵, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 79, 31 Aug. 2005
Japanese

フタトゲチマダニ由来新規メタロプロテアーゼ遺伝子のクローニングと性状解析
今村彩貴, 今内覚, 中島千絵, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 78, 31 Aug. 2005
Japanese

ザンビア共和国における牛およびRhipicephalus appendiculatusからのTheileria parvaの検出
今内覚, 今村彩貴, 中島千絵, SIMUNZA Martin, AMOS Chota, CHEMBENSOFU Mwelwa, ANDOREU Nambota, 安田準, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 84, 31 Aug. 2005
Japanese

鶏およびマガン由来マレック病ウイルスの遺伝子解析とその比較
村田史郎, 山本裕己, 岡田宰, 浅川満彦, 長雄一, 池内俊雄, 今内覚, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 108, 31 Aug. 2005
Japanese

発現遺伝子解析によるダニ由来有用物質の探索
中島千絵, 今村彩貴, 今内覚, 大橋和彦, 小沼操, 日本獣医学会学術集会講演要旨集, 138th, 65, 01 Aug. 2004
Japanese

BLV感染における遺伝的抵抗性(抵抗性牛)　　　　　　　　　　　　　　　
今内 覚, 臨床獣医, 22, 20, 23, Mar. 2004, [Invited], [Lead author, Corresponding author]
Japanese, Introduction scientific journal

Influence of polymorphism of MHC class II alleles on immune response in bovine leukemia virus infection.
Y Aida, S Konnai, S Takeshima, S Tajima, S Yin, M Onuma, K Okada, AIDS RESEARCH AND HUMAN RETROVIRUSES, 19, S18, S19, 2003
English, Summary international conference

Comparison of Peripheral Leukocytes in Japanese Black and Holstein Calves
OHTSUKA Hiromichi, KOMATSU Shoh-ichi, KON-NAI Satoru, FUKUDA Shigeo, KIKU Yoshio, YOSHINO Tomo-o, KOIWA Masateru, KAWAMURA Sei-ichi, Journal of the Japan Veterinary Medical Association, 55, 12, 789, 795, 20 Dec. 2002
黒毛和種(JB)子牛の細胞性免疫を評価する目的で、出生後のJB子牛（JB群；n=10）とホルスタイン種（Hol)子牛（Hol群；n=10）の末梢血白血球の変化を比較した。各群の子牛の出生後3日以内(0週齢)および3、6、9、12週齢の末梢血白血球はフローサイトメトリー（FCM)を用いて解析した。末梢血白血球はFCMの側方散乱光〔side light scatter(SSC)〕と前方散乱光〔forward light scatter (FSC)〕によって得られた3つの大きさ、すなわち小型、中型および大型に分類して単球・リンパ球（ML）とした。各群の中型（M)-ML数は0週齢が最低値で、その後増加した。しかしJB群のM-ML数は6から9週後に減少し、6週にはHol群に比べ有意な低値が認められた。JB群の小型（S)-ML数は3週後に最高値となり、0、3、9、12週にはHol群に比べ有意な高値が認められた。JB群のCD3(+)細胞数はHol群に比べ3週齢に有意な高値を示した。またS、M-ML中のWC(+)細胞比率は、観察期間中JB群がHol群に比べ低値を推移する傾向が認められた。これらのことからJB子牛の細胞性免疫応答はTリンパ球を中心にHol子牛とは異なることが明らかとなった。, 日本獸医師会, Japanese

OLA-DRB1アリルの塩基配列の決定とPCR-RFLP法の開発　　　　　　　　　　　　　　　
今内 覚, 長岡 淑子, 岡田 幸助, 小沼 操, 間 陽子, 日本獣医学会学術集会講演要旨集, 129回, 95, 95, Mar. 2000
(公社)日本獣医学会, Japanese

動物の感染症＜第5版＞　　　　　　　　　　　　　　　
今内 覚, 総論;IV感染症の予防と治療;感染症の予防;各論;牛 19.免疫不全ウイルス感染症;めん羊・山羊 9.跳躍病;10.ウェッセルスブロン病;11.羊肺腺腫
近代出版, Mar. 2025, [Contributor]

哺育・育成牛の飼養管理ガイド　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 第4章 給餌と飼養管理のポイント ③下痢予防のための発酵代用乳のつくり方と給与
DAIRYMAN, Oct. 2024, [Contributor]

哺育・育成牛の飼養管理ガイド　　　　　　　　　　　　　　　
今内 覚, 第3章 重要疾病の基礎知識と予防策 ①子牛の免疫とワクチン
DAIRYMAN, Oct. 2024, [Contributor]

動物衛生学 第2版　　　　　　　　　　　　　　　
今内 覚, 分担執筆, 範囲:コラム 牛伝染性リンパ腫 (旧名：牛白血病)
文永堂出版, Mar. 2024, [Joint work]

周産期疾病から牛を守る　　　　　　　　　　　　　　　
今内 覚 他, 第Ⅰ章 重要疾病の基礎知識と周産期免疫の新知見: ５ 周産期に免疫が低下するメカニズムの一端を解明
デーリィマン編集部, Nov. 2022, [Contributor]

獣医内科学第3版 産業動物編　　　　　　　　　　　　　　　
今内 覚, 第11章 血液・造血臓器疾患 5. 血液の感染症 1) 牛伝染性リンパ腫
一般社団法人 日本獣医内科アカデミー 編・文永堂出版, Mar. 2022, [Contributor]

主要症状を基礎にした牛の臨床３　　　　　　　　　　　　　　　
今内 覚 他, トリパノソーマ病、悪性カタル熱
デーリィマン社, May 2020, [Contributor]

Academic Fantasista 免疫療法で動物を救う　　　　　　　　　　　　　　　
今内 覚, 免疫療法で動物を救う https://www.hokudai.ac.jp/pr/hokudai_af2018.pdf
北海道大学, Apr. 2019, [Contributor]

動物の感染症＜第4版＞　　　　　　　　　　　　　　　
今内 覚, 総論:IV感染症の予防と治療: 1. 感染症の予防, 各論 : 11 アデノウイルス病, 19 牛免疫不全ウイルス感染症.
近代出版, Mar. 2019, [Contributor]

北大など、動物用の免疫チェックポイント阻害薬を開発 ー 目指すは病気の違いを越えた汎用免疫療法 ー　　　　　　　　　　　　　　　
今内 覚
日経バイオテクONLINE, Oct. 2017, [Others]

テレビ・ドクター４ よく分かる乳牛の病気100選　　　　　　　　　　　　　　　
今内 覚, 近年の重大疾病と予防策 地方病型(流行型)牛白血病、皮膚に異常を示す病気 散発性牛白血病の項
デーリィマン社, Oct. 2017, [Contributor]

動物衛生学　　　　　　　　　　　　　　　
今内 覚, コラム 牛白血病
文永堂出版, Sep. 2017, [Contributor]

動物用ワクチンとバイオ医薬品 -新たな潮流-　　　　　　　　　　　　　　　
Satoru Konnal, 第1章 総論 4. 抗体医薬: 免疫チェックポイント阻害薬等
文永堂出版, Jul. 2017, [Contributor]

牛白血病防除の観点から　　　　　　　　　　　　　　　
今内 覚, 検定検査乳S
検定検査乳S 34:7, Jan. 2016, [Others]

現代農業・牛白血病を出さない！尾鈴のBL対策　　　　　　　　　　　　　　　
宮崎県JA尾鈴, 牛白血病を出さない！尾鈴のBL対策
農山漁村文化協会, May 2015, [Others]

獣医免疫学　　　　　　　　　　　　　　　
今内 覚 ほか, 第13章 ワクチン
緑書房, 2015, [Contributor]

新製品の登場で注目集める動物用医薬品　　　　　　　　　　　　　　　
今内 覚
日経バイオテク, Sep. 2014, [Others]

牛病学〈第三版〉　　　　　　　　　　　　　　　
今内 覚, V. 感染症の制御 3) 免疫疲弊化と免疫賦活化
近代出版, 2013, [Contributor]

動物も新薬で病気から救える時代に　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話事業, 05 Dec. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫の現状から最後の砦：発症予測法について／プロバイオティクスを活用したワクチン効果の増強について　　　　　　　　　　　　　　　
今内 覚
令和7年度十勝獣医師会産業動物講習会・十勝管内家畜自衛防衛機推進協議会, 27 Nov. 2025, Japanese, Invited oral presentation
[Invited]

動物の難治性疾病に対する創薬研究 〜動物の免疫療法について〜　　　　　　　　　　　　　　　
今内 覚
日本実験動物医学会コラム(https://jalam.ne.jp/column/動物の難治性疾病に対する創薬研究-〜動物の免疫/), 12 Nov. 2025, Japanese, Others
[Invited]

プロバイオティクスで子牛のワクチン応答を増強 ～既存のワクチンプログラムへの応用に期待～　　　　　　　　　　　　　　　
今内 覚
プレスリリース(https://www.hokudai.ac.jp/news/pdf/251031_pr3.pdf), 31 Oct. 2025

牛伝染性リンパ腫の現状、発症早期診断法の開発とその実践　　　　　　　　　　　　　　　
今内 覚
公益社団法人福島県獣医師会令和７年度産業動物臨床講習会, 30 Oct. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫の現状、発症早期診断法の開発とその実践　　　　　　　　　　　　　　　
今内 覚
公益社団法人富山県獣医師会令和７年度家畜衛生対策事業, 15 Oct. 2025, Japanese, Invited oral presentation
[Invited]

動物難治性疾病に対する免疫療法の臨床研究　　　　　　　　　　　　　　　
今内 覚
日本実験動物医学会/JALAM特別講演「獣医領域における難治性疾患に対する新規治療法の開発」, 05 Sep. 2025, Japanese, Keynote oral presentation
[Invited]

牛伝染性リンパ腫の現状、発症早期診断法の開発とその実践　　　　　　　　　　　　　　　
今内 覚
宮城県農業共済組合令和7年度家畜診療等技術体験発表会及び診療技術研修会(第2班), 24 Jul. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫の現状、発症早期診断法の開発とその実践　　　　　　　　　　　　　　　
今内 覚
宮城県農業共済組合令和7年度家畜診療等技術体験発表会及び診療技術研修会(第１班), 23 Jul. 2025, Japanese, Invited oral presentation
[Invited]

Recent topics on enzootic bovine leukosis　　　　　　　　　　　　　　　
今内 覚
27th Summer Retrovirus Conference, 10 Jul. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫を巡る情勢とRAISING　　　　　　　　　　　　　　　
今内 覚
令和７年度徳之島地区牛伝染性リンパ腫研修会, 27 Jun. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫ウイルス感染症の防疫戦略　　　　　　　　　　　　　　　
今内 覚
令和７年度家畜生産農場衛生対策事業に係る牛伝染性リンパ腫対策検討会, 26 Jun. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫を巡る情勢とRAISING　　　　　　　　　　　　　　　
今内 覚
令和７年度家畜生産農場衛生対策事業に係る牛伝染性リンパ腫対策検討会, 25 Jun. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫の発症予測診断法’RAISING’とは　　　　　　　　　　　　　　　
今内 覚
オホーツク獣医師会獣医新技術講習会, 22 May 2025, Invited oral presentation
[Invited]

古くて新しいプロバイオティクス ～最近の話題について～　　　　　　　　　　　　　　　
今内 覚
オホーツク獣医師会獣医新技術講習会, 22 May 2025, Japanese, Invited oral presentation
[Invited]

イヌのがんに抗CTLA-４抗体治療が有効であることを初めて報告 ～イヌのがんへの免疫療法の適用拡大に期待～　　　　　　　　　　　　　　　
今内 覚
プレスリリース(https://www.hokudai.ac.jp/news/pdf/250520_pr2.pdf), 20 May 2025

牛リンパ腫発症予測診断技術 RAISING の精度の高さを証明 ～国内初の 14 研究機関による多施設検証試験を実施～　　　　　　　　　　　　　　　
今内 覚
プレスリリース（https://www.hokudai.ac.jp/news/pdf/250328_pr.pdf）, 28 Mar. 2025, Japanese

牛伝染性リンパ腫を巡る情勢　　　　　　　　　　　　　　　
今内 覚
独立行政法人家畜改良センター新冠牧場 牛の飼養・衛生管理に関するセミナー, 26 Mar. 2025, Japanese, Invited oral presentation
[Invited]

抗菌剤に頼らない畜産物の生産体制の推進 薬剤耐性菌問題の克服も目指した新たな動物疾病制御法の実践研究　　　　　　　　　　　　　　　
今内 覚
知の集積と活用の場 産学官連携協議会, 14 Feb. 2025, Japanese, Public symposium

牛伝染性リンパ腫の現状、発症早期診断法の開発とその実践　　　　　　　　　　　　　　　
今内 覚
第42回日本獣医師会獣医学術学会年次大会（令和6年度）, 26 Jan. 2025, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫診断の新技術・早期発症予測診断法 RAISING とは　　　　　　　　　　　　　　　
今内 覚
第66回岐阜県家畜保健衛生業績発表会, 20 Dec. 2024, Japanese, Invited oral presentation
[Invited]

古くて新しいプロバイオティクス　　　　　　　　　　　　　　　
今内 覚
2024 アグリビジネス創出フェア in Hokkaido －北海道の食と農の明日へ－, 08 Nov. 2024, Japanese, Public symposium

動物も新薬で病気から救える時代に　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話事業, 25 Oct. 2024, Japanese, Invited oral presentation
[Invited]

イヌの腫瘍に対する免疫療法の試み　　　　　　　　　　　　　　　
今内 覚
北海道獣医師会十勝支部小動物講習会, 19 Oct. 2024, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫発症牛の全部廃棄大幅削減による日本経済の活性化～前がん細胞検診技術RAISINGの実用化～　　　　　　　　　　　　　　　
今内 覚
鹿児島県家畜畜産物衛生指導協会令和6年度家畜生産農場衛生対策事業に係る牛伝染性リンパ種対策講習会, 26 Sep. 2024, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫発症牛の全部廃棄大幅削減による日本経済の活性化～前がん細胞検診技術RAISINGの実用化～　　　　　　　　　　　　　　　
今内 覚
第6回南九州畜産獣医学拠点(SKLV：すくらぶ)セミナー, 25 Sep. 2024, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫の現状と発症予測法　　　　　　　　　　　　　　　
今内 覚
第64回獣医疫学会学術集会シンポジウム, 31 Aug. 2024, Japanese, Invited oral presentation
[Invited]

古くて新しいプロバイオティクス 〜最近の話題について〜　　　　　　　　　　　　　　　
今内 覚
北海道獣医師会北海道地区学会セミナー, 29 Aug. 2024, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫について 感染・発症・診断の基本と最前線　　　　　　　　　　　　　　　
今内 覚
東京都芝浦食肉衛生検査所研修会, 23 May 2024, Japanese, Invited oral presentation
[Invited]

Immune Checkpoint Inhibitors 〜Breakthrough Cancer Therapy and its Application to Veterinary Medicine 〜　　　　　　　　　　　　　　　
Satoru KONNAI
Seminario Especial in Universidade Federal do Rio Grande do Sul, 19 Mar. 2024, English, Invited oral presentation
[Invited]

牛伝染性リンパ腫の現状から最後の砦：発症予測法について　　　　　　　　　　　　　　　
今内 覚
新潟県獣医師会産業動物部会特別講習会, 01 Mar. 2024, Japanese, Invited oral presentation
[Invited]

動物も新薬で病気から救える時代に　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話事業, 21 Feb. 2024, Japanese, Invited oral presentation
[Invited]

獣医学から生まれる医学への貢献 汎動物学(ズービキティ）とは？　　　　　　　　　　　　　　　
今内 覚
One Health リサーチセンター 開所記念 市民公開講座, 17 Feb. 2024, Japanese, Invited oral presentation

牛伝染性リンパ腫ってどういう病気？ 感染・発症・診断の基本と最前線　　　　　　　　　　　　　　　
今内 覚
細胞診クラブ主催 牛伝染性リンパ腫フォーラム【基本と最前線×東洋医学的解析×鍼灸治療】, 20 Jan. 2024, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫の現状から最後の砦：発症予測法について　　　　　　　　　　　　　　　
今内 覚
第41回日本獣医師会獣医学術学会年次大会（令和5年度）特別企画, 03 Dec. 2023, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫とは？ 最後の砦！発症予測診断法RAISINGとは？　　　　　　　　　　　　　　　
今内 覚
2023アグリビジネス創出フェア in Hokkaido －北海道の食と農の明日へ－, 09 Nov. 2023, Japanese, Public symposium

早期発症診断によって経済損失低減を実現する 牛のがん検診　　　　　　　　　　　　　　　
今内 覚
知の集積と活用の場 産学官連携協議会2023, 07 Nov. 2023, Japanese, Public symposium

Novel livestock disease prevention method using probiotics instead of antibiotics　　　　　　　　　　　　　　　
Satoru KONNAI
7th International Livestock Biotechnology Symposium, 19 Oct. 2023, English, Invited oral presentation
17 Oct. 2023 - 20 Oct. 2023, [Invited]

牛伝染性リンパ腫対策の最新知見　　　　　　　　　　　　　　　
今内 覚
令和5年岩手県獣医師会研修会, 13 Oct. 2023, Japanese, Invited oral presentation
13 Oct. 2023, [Invited]

イヌの鼻腔内腺癌や骨肉腫に免疫チェックポイント阻害剤が有効であることを初めて報告 ～イヌ用抗PD-L1抗体による免疫療法の適用拡大に期待～　　　　　　　　　　　　　　　
今内 覚ら
プレスリリース(https://www.hokudai.ac.jp/news/pdf/231005_pr.pdf), 05 Oct. 2023, Japanese, Others

酪農教育ファームにおける安全・衛生　　　　　　　　　　　　　　　
今内 覚
中央酪農会議 令和5年度酪農教育ファームスキルアップ研修会, 22 Sep. 2023, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫における最近の知見、新たな発症予測診断について　　　　　　　　　　　　　　　
今内 覚
北海道獣医師会留萌支部獣医新技術講習会, 20 Sep. 2023, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫への対策に向けて　　　　　　　　　　　　　　　
今内 覚
松前町肉牛改良センター特別講演会, 28 Jun. 2023, Japanese, Invited oral presentation
[Invited]

Combination therapy effective against canine melanoma　　　　　　　　　　　　　　　
Konnai S
Research Press Release (https://www.global.hokudai.ac.jp/blog/combination-therapy-effective-against-canine-melanoma/), 23 Jun. 2023

イヌ悪性黒色腫に対して放射線治療との併用で 抗PD-L1抗体の効果が高まることをはじめて報告 ～イヌ用免疫チェックポイント阻害薬のより良い使用法の実現に期待～　　　　　　　　　　　　　　　
今内 覚ら
プレスリリース(https://www.hokudai.ac.jp/news/pdf/230602_pr.pdf), 02 Jun. 2023

動物難治性疾病に対する新規予防・治療法の開発　　　　　　　　　　　　　　　
今内 覚
第15回大動物臨床研究会特別セミナー, 26 May 2023, Japanese, Invited oral presentation
[Invited]

動物難治性疾病に対する新規予防・治療法の開発　　　　　　　　　　　　　　　
今内 覚
北里大学第486回獣医学科セミナー＜特別講演＞, 18 May 2023, Japanese, Invited oral presentation
[Invited]

牛のリンパ腫発症を予測するがん検診技術　　　　　　　　　　　　　　　
今内 覚
岩手県中央家畜保健衛生所セミナー, 27 Apr. 2023, Japanese, Invited oral presentation
[Invited]

PD-L1の阻害により既存のワクチン効果を増強 ～子牛のワクチンプログラムへの応用に期待～　　　　　　　　　　　　　　　
今内 覚ら
プレスリリース(https://www.hokudai.ac.jp/news/pdf/230316_pr2.pdf), 16 Mar. 2023

動物も新薬で病気から救える時代に　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話事業, 14 Mar. 2023, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫 (旧名：牛白血病)の現状と早期発症診断による経済損失低減の実現　　　　　　　　　　　　　　　
今内 覚
山口県畜産振興協会令和4年度家畜生産農場衛生対策事業, 21 Feb. 2023, Japanese, Invited oral presentation
[Invited]

Development of effective preventive and therapeutic methods for animal diseases　　　　　　　　　　　　　　　
Satoru KONNAI
Special seminar in Mongolian University of Life Sciences, 15 Feb. 2023, English, Invited oral presentation
[Invited]

動物も新薬で病気から救える時代に　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話事業, 09 Nov. 2022, Japanese, Invited oral presentation
[Invited]

牛のリンパ腫発症を予測するがん検診技術を開発～発症予測法の実用化による畜産被害の軽減に期待～　　　　　　　　　　　　　　　
今内 覚ら
プレスリリース(https://www.hokudai.ac.jp/news/2022/10/post-1113.html), 21 Oct. 2022

Breakthrough in cancer therapy　　　　　　　　　　　　　　　
Satoru KONNAI
Capability building of ASEAN biotechnology researchers for livestock resiliency and sustainability, 05 Jul. 2022, English, Invited oral presentation
[Invited]

Importance of building global network for livestock resiliency and sustainability　　　　　　　　　　　　　　　
Satoru KONNAI
Capability building of ASEAN biotechnology researchers for livestock resiliency and sustainability, 04 Jul. 2022, English, Invited oral presentation
[Invited]

Novel livestock disease prevention method using probiotics instead of antibiotics　　　　　　　　　　　　　　　
Satoru KONNAI
Capability building of ASEAN biotechnology researchers for livestock resiliency and sustainability, 04 Jul. 2022, English, Invited oral presentation
[Invited]

家畜伝染病ヨーネ病の現状と病態発生機序について　　　　　　　　　　　　　　　
今内 覚
第97回日本結核・非結核性抗酸菌症学会学術講演会エキスパートセミナー, 01 Jul. 2022, Japanese, Invited oral presentation
[Invited]

動物難治性疾病に対する治療と実際、未来　　　　　　　　　　　　　　　
今内 覚
北里大学第471回獣医学科セミナー＜Cutting edge＞, 16 May 2022, Invited oral presentation
16 May 2022 - 16 May 2022, [Invited]

母牛はわが身を削って子牛を出産する～牛伝染性リンパ腫と分娩との関係，周産期に疾病が多発するメカニズムの一端を証明～　　　　　　　　　　　　　　　
今内 覚ら
プレスリリース(https://www.hokudai.ac.jp/news/pdf/220310_pr.pdf)(https://www.hokudai.ac.jp/news/2022/03/post-1006.html), 10 Mar. 2022, Japanese, Others

牛伝染性リンパ腫の防除戦略　　　　　　　　　　　　　　　
今内 覚
全国農業共済協会 令和3年度 家畜診療等技術全国研究集会, 22 Feb. 2022, Japanese, Invited oral presentation
[Invited]

動物用バイオ医薬品の開発と応用研究　　　　　　　　　　　　　　　
今内 覚
令和3年度日本薬剤師会農林水産薬事薬剤師部会動物薬事研修会, 18 Feb. 2022, Japanese, Invited oral presentation
[Invited]

動物難治性疾病に対する治療と実際、未来　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話事業, 10 Feb. 2022, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫における免疫学的解析を基盤とした新制御法の試み　　　　　　　　　　　　　　　
今内 覚
日本獣医学会市民公開講座, 05 Feb. 2022, Japanese, Invited oral presentation
[Invited]

動物難治性疾病に対する治療と実際、未来　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話事業, 04 Feb. 2022, Japanese, Invited oral presentation
[Invited]

牛伝染性リンパ腫の病態発生機序解析を基盤とする新制御法の試み　　　　　　　　　　　　　　　
今内 覚
日本家畜衛生学会 第94回大会・家畜衛生フォーラム2021, 03 Dec. 2021, Japanese, Invited oral presentation
03 Dec. 2021, [Invited]

牛伝性性リンパ腫の最新知見について　　　　　　　　　　　　　　　
今内 覚
令和3年度家畜保健衛生所病性鑑定技術検討会, 16 Nov. 2021, Japanese, Invited oral presentation
16 Nov. 2021 - 16 Nov. 2021, [Invited]

牛伝染性リンパ腫の現状と対策　　　　　　　　　　　　　　　
今内 覚
ささえや製薬学術セミナー, 27 Oct. 2021, Japanese, Invited oral presentation
27 Oct. 2021 - 27 Oct. 2021, [Invited]

動物の慢性感染症と免疫チェックポイント分子　　　　　　　　　　　　　　　
今内 覚
十勝家畜保健衛生所講演会, 30 Sep. 2021, Japanese, Invited oral presentation
30 Sep. 2021 - 30 Sep. 2021, [Invited]

牛伝染性リンパ腫の新たな知見　　　　　　　　　　　　　　　
今内 覚
北海道獣医師会十勝支部産業講習会, 29 Sep. 2021, Japanese, Invited oral presentation
29 Sep. 2021 - 29 Sep. 2021, [Invited]

牛伝染性リンパ腫の最新知見　　　　　　　　　　　　　　　
今内 覚
オホーツク獣医師会獣医新技術講習会, 20 Jul. 2021, Japanese, Invited oral presentation
20 Jul. 2021 - 20 Jul. 2021, [Invited]

免疫チェックポイント阻害薬による腫瘍免疫療法について 〜北海道大学動物医療センターにおける臨床研究成果から〜　　　　　　　　　　　　　　　
今内 覚
One Healthリサーチセンター キックオフシンポジウム, 29 May 2021, Japanese, Invited oral presentation
29 May 2021 - 29 May 2021, [Invited]

Research Press Release: New therapy target for malignant melanomas in dogs　　　　　　　　　　　　　　　
Konnai S
Research Press Release (https://www.global.hokudai.ac.jp/blog/new-therapy-target-for-malignant-melanomas-in-dogs/), 22 Apr. 2021, English, Media report

Probiotics keep calves healthy, too!　　　　　　　　　　　　　　　
Probiotics keep, calves healthy, too
Research Press Release (https://www.global.hokudai.ac.jp/blog/probiotics-keep-calves-healthy-too/), 06 Apr. 2021, English, Media report

続報・肺転移のあるイヌ悪性黒色腫に抗PD-L1抗体が有効であることをはじめて実証 ～イヌ用免疫チェックポイント阻害薬の実現に大きく前進～　　　　　　　　　　　　　　　
今内 覚, 前川直也ら
プレスリリース(https://www.hokudai.ac.jp/news/pdf/210215_pr.pdf) (https://www.hokudai.ac.jp/news/2021/02/pd-l1.html), 15 Feb. 2021, Japanese, Media report

Deeper insight into how tick spit suppresses cattle immunity　　　　　　　　　　　　　　　
Konnai S
Research Press Release (https://www.global.hokudai.ac.jp/blog/deeper-insight-into-how-tick-spit-suppresses-cattle-immunity/) (https://conexao.ufrj.br/2021/01/28/parceria-nipo-brasileira-estuda-como-carrapatos-afetam-bovinos/) (https://www.correiodopovo.com.br/especial/chance-de-al%C3%ADvio-para-bovinos-1.573426), 28 Jan. 2021, English, Media report

プロバイオティクスで子牛を下痢から守る～発酵哺乳飼料による子牛の腸炎防御効果を証明～　　　　　　　　　　　　　　　
今内 覚, 茅先 史, 岡川朋弘ら
プレスリリース (https://www.hokudai.ac.jp/news/pdf/210118_pr.pdf) (https://www.hokudai.ac.jp/news/2021/01/post-783.html), 18 Jan. 2021, Japanese, Media report

マダニ唾液が免疫チェックポイント因子の発現を誘導～マダニ媒介性病原体の伝播機序の解明に期待～　　　　　　　　　　　　　　　
今内 覚, 佐治木大和ら
プレスリリース (https://www.hokudai.ac.jp/news/pdf/210114_pr3.pdf)(https://www.hokudai.ac.jp/news/2021/01/post-779.html), 14 Jan. 2021, Japanese, Media report

プロスタグランジンE2を介した免疫チェックポイント阻害薬の新たな耐性獲得機構の解明～新たな免疫療法への応用に期待～　　　　　　　　　　　　　　　
今内 覚, 佐治木大和ら
プレスリリース(https://www.hokudai.ac.jp/news/pdf/201222_pr.pdf)(https://www.hokudai.ac.jp/news/2020/12/e2.html), 21 Dec. 2020, Japanese, Media report

子牛の下痢症に対する発酵代用乳の腸炎抑制効果の検証　　　　　　　　　　　　　　　
今内 覚
令和2年度獣医療提供体制整備推進総合対策事業：新規獣医師臨床研修促進事業 実践実習, 28 Oct. 2020, Japanese, Invited oral presentation
28 Oct. 2020, [Invited]

家畜の慢性感染症制御の難しさ 〜免疫から逃れる病原体たち〜　　　　　　　　　　　　　　　
今内 覚
令和2年度獣医療提供体制整備推進総合対策事業：新規獣医師臨床研修促進事業, 28 Oct. 2020, Japanese, Invited oral presentation
28 Oct. 2020, [Invited]

牛伝染性リンパ腫(旧名：牛白血病) の最新知見 北海道大学による臨床検査診断結果に基づく実践的対策の紹介　　　　　　　　　　　　　　　
今内 覚
令和2年家畜診療等技術北海道地区研修会, 28 Oct. 2020, Japanese, Invited oral presentation
28 Oct. 2020, [Invited]

動物用バイオ医薬品実用化を可能とする大量生成技術の構築　　　　　　　　　　　　　　　
今内 覚
イノベーション創出強化研究推進事業研究紹介2020 (http://www.naro.affrc.go.jp/laboratory/brain/innovation/results/2019/26058BC.html), 23 Oct. 2020, Japanese, Media report
[Invited]

免疫療法 〜免疫チェックポイント阻害剤について〜　　　　　　　　　　　　　　　
今内 覚
北海道大学One Healthフロンティア卓越大学院プログラムOne Health Relay Report https://onehealth.vetmed.hokudai.ac.jp/news/220, 16 Mar. 2020, Japanese, Others
[Invited]

動物の難病(がん・感染症)に対する創薬研究　　　　　　　　　　　　　　　
今内 覚
日本学術振興会 科研費 研究成果トピックス https://www.jsps.go.jp/j-grantsinaid/37_topics/data/10101-40396304.pdf, 16 Mar. 2020, Japanese, Media report
[Invited]

動物用バイオ医薬品の開発と応用研究　　　　　　　　　　　　　　　
今内 覚
農林水産省動物医薬品検査所 令和元年度第１回特別講演会, 24 Jan. 2020, Japanese, Invited oral presentation
[Invited]

牛白血病の最新知見　　　　　　　　　　　　　　　
今内 覚
北海道獣医師会宗谷支部令和元年度新技術講習会, 19 Nov. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病の最新知見〜北海道大学臨床検査診断結果に基づく実践的対策の紹介〜　　　　　　　　　　　　　　　
今内 覚
みなみ北海道農業共済組合南部家畜診療センター講習会, 05 Nov. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

抗生剤に頼らないプロバイオティクスを応用した新規家畜疾病予防法の開発 〜発酵哺乳飼料による子牛の下痢抑制効果〜　　　　　　　　　　　　　　　
今内 覚
みなみ北海道農業共済組合南部家畜診療センター講習会, 05 Nov. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物の難病(がん・感染症)に対する 創薬研究(新薬の開発)　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話, 24 Oct. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物用抗体医薬品の開発および臨床研究　　　　　　　　　　　　　　　
今内 覚
公益社団法人日本動物用医薬品協会第51回学術講習会, 16 Oct. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物難治性疾病に対する創薬研究　　　　　　　　　　　　　　　
今内 覚
WISE特別セミナー2019, 08 Oct. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy　　　　　　　　　　　　　　　
Fujisawa S, Konnai S, Okagawa T, Maekawa N, Goto S, Murata S, Ohashi K
International Veterinary Immunology Symposium 2019, 16 Aug. 2019, English, Oral presentation
[International presentation]

Functional analysis of bovine CTLA-4 in bovine leukemia virus infection　　　　　　　　　　　　　　　
Watari K, Konnai S, Okagawa T, Maekawa N, Goto S, Murata S, Ohashi K
International Veterinary Immunology Symposium 2019, 16 Aug. 2019, English, Oral presentation
[International presentation]

Development of a sensitive PD-L1 immunohistochemistry of canine cancers and clinical efficacy of an anti-PD-L1 antibody in canine oral malignant melanoma with pulmonary metastasis　　　　　　　　　　　　　　　
Maekawa N, Konnai S, Okagawa T, Murata S, Ohashi K
International Veterinary Immunology Symposium 2019, 16 Aug. 2019, English, Invited oral presentation
[International presentation]

Immunomodulatory effects of sialostatin L and sialostatin L2 from Ixodes persulcatus Schulze, Taiga tick　　　　　　　　　　　　　　　
Satoru Konnai, Yamato Sajiki, Tomohiro Okagawa, Naoya Maekawa, Masayoshi Isezaki, Shiro Murata, Kazuhiko Ohashi
International Veterinary Immunology Symposium 2019, 16 Aug. 2019, English, Oral presentation
[International presentation]

The induction of immunosuppression via prostaglandin E2 and the enhancement of anti-bacterial effects by anti-PD-L1 antibody combined with COX-2 inhibitor in Mycoplasma bovis infection　　　　　　　　　　　　　　　
Goto S, Konnai S, Okagawa T, Maekawa N, Murata S, Ohashi K
International Veterinary Immunology Symposium 2019, 15 Aug. 2019, English, Oral presentation
[International presentation]

Contribution of prostaglandin E2 to disease progression and enhancement of antiviral effects by anti-PD-L1 antibody combined with COX-2 inhibitor in bovine leukemia virus infection　　　　　　　　　　　　　　　
Sajiki Y, Konnai S, Okagawa T, Maekawa N, Murata S, Ohashi K
International Veterinary Immunology Symposium 2019, 14 Aug. 2019, English, Invited oral presentation
[International presentation]

Establishment of anti-bovine PD-1 chimeric antibody and a pilot clinical study　　　　　　　　　　　　　　　
Satoru Konnal, Tomohiro Okagawa, Naoya Maekawa, Shiro Murata, Kazuhiko Ohashi
International Veterinary Immunology Symposium 2019, 14 Aug. 2019, English, Invited oral presentation
[International presentation]

ウシマイコプラズマ感染症における免疫疲弊化　　　　　　　　　　　　　　　
今内 覚
日本マイコプラズマ学会第46会学術集会, 24 May 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病臨床検査診断結果に基づく実践的対策　　　　　　　　　　　　　　　
今内 覚
上川青年獣医師会特別講演, 22 May 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

獣医学領域における感染症の制御　　　　　　　　　　　　　　　
今内 覚
微生物化学研究所特別講演, 13 May 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

免疫チェックポイントを標的としたイヌ免疫療法の試み　　　　　　　　　　　　　　　
今内 覚
麻布大学獣医学部臨床特別セミナー, 16 Apr. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Developing immunotherapy to save animals　　　　　　　　　　　　　　　
Satoru Konnai
Research Highlight: https://www.global.hokudai.ac.jp/blog/developing-immunotherapy-to-save-animals/, 08 Apr. 2019, English, Media report
[Invited], [International presentation]

動物難治性疾病に対する創薬研究　　　　　　　　　　　　　　　
今内 覚
東北大学大学院医学系研究院抗体創薬研究分野 第6回抗体創薬研究セミナー, 26 Mar. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Six scientists whose transformative work reaches across borders.　　　　　　　　　　　　　　　
Satoru Konnai
Nature index: https://www.nature.com/articles/d41586-019-00831-5, 20 Mar. 2019, English, Media report
[Invited], [International presentation]

牛白血病の最新知見とウイルス量を基盤とした対策について　　　　　　　　　　　　　　　
今内 覚
大分県畜産協会家畜生産農場清浄化支援事業講習会, 13 Mar. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

静かなる脅威・牛白血病 〜最新知見から〜　　　　　　　　　　　　　　　
今内 覚
日本中央競馬会(JRA)畜産振興事業シンポジウム 地方病性牛白血病・清浄化へのアプローチ ~牛白血病清浄化に向けて今しなければならないこと~, 06 Mar. 2019, Japanese, Keynote oral presentation
[Invited], [Domestic Conference]

家畜の慢性感染症制御の難しさ 〜免疫から逃れる病原体たち〜　　　　　　　　　　　　　　　
今内 覚
第9回大動物臨床研究会東京シンポジウム, 02 Mar. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物難治性疾病に対する創薬研究 〜免疫チェックポイントを標的としたイヌ免疫療法の試み〜　　　　　　　　　　　　　　　
今内 覚
第15回日本獣医内科学アカデミー学術大会, 16 Feb. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Immune checkpoint inhibitors-related diseases in animals: Application of immunotherapy targeting the immune checkpoints for veterinary medicine　　　　　　　　　　　　　　　
Satoru KONNAI
Seminario Especial in Universidade Federal do Rio Grande do Sul, 08 Feb. 2019, English, Invited oral presentation
[Invited], [International presentation]

動物の難病(がん・感染症)に対する 創薬研究(新薬の開発)　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話, 26 Jan. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病臨床検査診断結果に基づく実践的対策　　　　　　　　　　　　　　　
Satoru Konnal
平成30年度小平町和牛生産改良組合牛白血病研修会, 16 Jan. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物難治性疾病に対する創薬研究 〜免疫チェックポイントを標的とした新規免疫療法の試み〜　　　　　　　　　　　　　　　
Satoru Konnal
大阪市立大学大学院工学研究科 医工・生命工学教育研究センター第１回セミナー, 27 Dec. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

イヌ難治性腫瘍疾患に対する免疫チェックポイントを 標的とした新規免疫療法の試み　　　　　　　　　　　　　　　
今内 覚, 前川直也, 細谷謙次, 金 尚昊, 高木 哲, 賀川由美子, 岡川朋弘, 村田史郎, 大橋和彦
北海道小動物獣医師会年次大会2018, 04 Nov. 2018, Japanese, Oral presentation
[Domestic Conference]

牛白血病の最新知見、現状と対策　　　　　　　　　　　　　　　
今内 覚
兵庫県畜産協会平成30年度食の安全・消費者の信頼確保対策事業平成30年度牛白血病講習会, 23 Oct. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物難治性疾病に対する創薬研究 〜免疫学的解析を基盤とした新規制御法の開発〜　　　　　　　　　　　　　　　
今内 覚
第161回日本獣医学会学術集会微生物学分科会シンポジウム「感染症対策の新たな突破口を探る」, 11 Sep. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病の最新知見 〜臨床データーからの更新知見〜　　　　　　　　　　　　　　　
今内 覚
平成30年度みなみ北海道農業共済組合第1回診療等体験発表会 特別講演, 26 Jul. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

免疫チェックポイントを標的とした動物用抗体医薬の開発　　　　　　　　　　　　　　　
今内 覚
第2回ヒトと伴侶動物の比較医学研究会 ヒト医療と獣医療の先端技術を駆使した次世代のがん治療〜がん撲滅への新たな挑戦〜, 30 Jun. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛難治性疾病における免疫疲弊化機序の 解析と制御法への応用　　　　　　　　　　　　　　　
今内 覚
第74回九州・山口病性鑑定協議会, 21 Jun. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物難治性疾病に対する創薬研究　　　　　　　　　　　　　　　
今内 覚
北里大学獣医学部特定講義, 06 Jun. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病の最新知見 現状と対策　　　　　　　　　　　　　　　
今内 覚
国立大学法人岐阜大学応用生物科学部附属家畜衛生地域連携教育研究センター家畜衛生講演会, 04 Jun. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病の最新知見　　　　　　　　　　　　　　　
今内 覚
鹿児島県獣医師会 産業動物部会曽於・肝属地区獣医師講習会, 27 Mar. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病の最新知見 帯広食肉衛生検査所との共同研究成果から　　　　　　　　　　　　　　　
今内 覚
北海道獣医師会十勝支部公衆衛生講習会, 07 Mar. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛白血病の疫学と公衆衛生分野での留意点　　　　　　　　　　　　　　　
今内 覚
北海道獣医師会平成29年度獣医公衆衛生講習会, 05 Mar. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

地方病性牛白血病対策 について　　　　　　　　　　　　　　　
今内 覚
平成29年度岩手県牛白血病防疫推進講習会, 26 Jan. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

牛の免疫応答を利用した難治性疾病の新規制御法開発 〜免疫チェックポイントを標的とした動物用抗体創薬〜　　　　　　　　　　　　　　　
今内 覚
家畜衛生フォーラム2017 「抗菌剤に頼らない新しい家畜疾病の制御法 －モデルとしての難治性・慢性疾病克服のための研究－」, 15 Dec. 2017, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Immune exhaustion during chronic diseases in animals　　　　　　　　　　　　　　　
Satoru Konnal
The 20th Joint Symposium Between Seoul National University and Hokkaido University, 16 Nov. 2017, English, Invited oral presentation
[Invited], [International presentation]

牛難治性疾病に対する多機能型バイオ医薬(抗体医薬)の創出と発展的応用　　　　　　　　　　　　　　　
今内 覚
農林水産業・食品産業科学技術研究推進事業 「知」の集積と活用の場ポスターセッション, 10 Nov. 2017, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

動物用バイオ医薬品の開発　　　　　　　　　　　　　　　
今内 覚
東北大学大学院医学系研究科抗体創薬研究分野 第二回抗体創薬研究セミナー, 02 Nov. 2017, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

増加している牛白血病 - 北海道での現状と対策について -　　　　　　　　　　　　　　　
今内 覚
第22回北海道肉牛研究会大会, 26 Oct. 2017, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Epidemiological and immunological study for intractable infectious diseases in Mongolian livestock　　　　　　　　　　　　　　　
Satoru KONNAI
JICA Project: Infectious Diseases Seminar 2017 in Mongolia, 10 Oct. 2017, English, Invited oral presentation
[Invited], [International presentation]

動物の難病に対する新規治療法の開発　　　　　　　　　　　　　　　
今内 覚
国民との科学・技術対話, 03 Oct. 2017, Japanese, Public discourse
[Invited], [Domestic Conference]

Theileria parva感染症における免疫チェックポイント因子の動態解析　　　　　　　　　　　　　　　
今内 覚, 岡川朋弘, 山田慎二, Martin Simuunza, Timothy Connelley, Ivan Morrison, 村田史郎, 大橋和彦
第160回日本獣医学会学術集会, 13 Sep. 2017, Japanese, Oral presentation
[Domestic Conference]

Role of Inhibitory Molecules in Bovine Chronic Infectious Diseases and as Target for Therapy　　　　　　　　　　　　　　　
Satoru Konnal
宮崎大学産業動物防疫リサーチセンター(CADIC)特別セミナー, 11 Sep. 2017, English, Invited oral presentation
[Invited], [Domestic Conference]

Therapeutic intervention in cancer and chronic infections in animals: Antibody mediated manipulation of PD-1/PD-L1 interaction　　　　　　　　　　　　　　　
Satoru Konnal
Hokkaido University & Colorado State University Kick-off Symposium 2017, 26 Jul. 2017, English, Invited oral presentation
[Invited], [International presentation]