Jul. 2024, The Cellulose Society of Japan, Prize from the Cellulose Society of Japan　　　　　　　　　　　　　　　
Kenji Tajima

May 2015, 製糖技術研究会, 第112回製糖技術研究会賞　　　　　　　　　　　　　　　
田島健次、他

Jul. 2010, セルロース学会, 林治助賞　　　　　　　　　　　　　　　
Japan

Aug. 2004, International Symposium on Bio Polyesters 2004, Poster award　　　　　　　　　　　　　　　
TAJIMA Kenji

Sep. 2002, 高分子化学奨励賞<平成１４年度>　　　　　　　　　　　　　　　
Japan

Molecular structure of enzyme-synthesized amylose-like chimeric isomaltomegalosaccharides and their encapsulation of the sulfasalazine prodrug
Weeranuch Lang, Yoshiaki Yuguchi, Chun-Yao Ke, Ting-Wei Chang, Yuya Kumagai, Wilaiwan Kaenying, Takayoshi Tagami, Feng Li, Takuya Yamamoto, Kenji Tajima, Kenji Takahashi, Takuya Isono, Toshifumi Satoh, Atsuo Kimura
Carbohydrate Polymers, 122956, 122956, Elsevier BV, Nov. 2024, [Peer-reviewed]
English, Scientific journal

Poly(butylene succinate) reinforced by small amount of grafted nanofibrillated bacterial cellulose: Toughness variability based on nanocomposites preparation method
Hamidah binti Hashim, Xiaochao Xia, Hiroshi Kani, Shuichiro Seno, Feng Li, Takuya Isono, Takuya Yamamoto, Hirofumi Tani, Toshifumi Satoh, Kenji Tajima
Composites Part A: Applied Science and Manufacturing, 185, 108341, 108341, Elsevier BV, Oct. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Polyester Adhesives via One-Pot, One-Step Copolymerization of Cyclic Anhydride, Epoxide, and Lactide
Ryota Suzuki, Toshiki Miwa, Ryosuke Nunokawa, Ayaka Sumi, Masaru Ando, Katsuaki Takahashi, Akira Takagi, Takuya Yamamoto, Kenji Tajima, Feng Li, Takuya Isono, Toshifumi Satoh
Polymers, 16, 19, 2767, 2767, MDPI AG, 30 Sep. 2024, [Peer-reviewed]
English, Scientific journal, Polyesters (PEs) are sustainable alternatives for conventional polymers owing to their potential degradability, recyclability, and the wide availability of bio-based monomers for their synthesis. Herein, we used a one-pot, one-step self-switchable polymerization linking the ring-opening alternating copolymerization (ROAC) of epoxides/cyclic anhydrides with the ring-opening polymerization (ROP) of L-lactide (LLA) to synthesize PE-based hot-melt adhesives with a high bio-based content. In the cesium pivalate-catalyzed self-switchable polymerization of glutaric anhydride (GA), butylene oxide (BO), and LLA using a diol initiator, the ROAC of GA and BO proceeded whereas the ROP of LLA simultaneously proceeded very slowly, resulting in a copolyester consisting of poly(GA-alt-BO) and poly(L-lactide) (PLLA) segments with tapered regions, that is, PLLA-tapered block-poly(GA-alt-BO)-tapered block-PLLA (PLLA-tb-poly(GA-alt-BO)-tb-PLLA). Additionally, a series of tapered-block or real-block copolyesters consisting of poly(anhydride-alt-epoxide) (A segment) and PLLA (B segment) with AB-, BAB-, (AB)3-, and (AB)4-type architectures of different compositions and molecular weights were synthesized by varying the monomer combinations, alcohol initiators, and initial feed ratios. The lap shear tests of these copolyesters revealed an excellent relationship between the adhesive strength and polymer structural parameters. The (AB)4-type star-block copolyester (poly(GA-alt-BO)-tb-PLLA)4 exhibited the best adhesive strength (6.74 ± 0.64 MPa), comparable to that of commercial products, such as PE-based and poly(vinyl acetate)-based hot-melt adhesives.

Application of bacterial-derived long cellulose nanofiber to suspension culture of mammalian cells as a shear protectant
Eiichiro Kaneko, Haruto Tsujisaki, Masashi Fujiwara, Hidenori Ando, Yasushi Sato, Tatsuhiro Ishida, Hirofumi Tani, Kenji Tajima
International Journal of Biological Macromolecules, 135938, 135938, Elsevier BV, Sep. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Detailed structural analyses and viscoelastic properties of nano-fibrillated bacterial celluloses
Haruto Tsujisaki, Masaaki Hosokawa, Yuichi Takasaki, Yoshifumi Yamagata, Yui Kawabata, Daisuke Tatsumi, Shuichiro Seno, Keisuke Miyamoto, Takuya Isono, Takuya Yamamoto, Hirofumi Tani, Toshifumi Satoh, Hiroshi Orihara, Kenji Tajima
Carbohydrate Polymer Technologies and Applications, 100565, 100565, Elsevier BV, Sep. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 34012607

Bacterial Cellulose Production in Space
Akiko TANAKA, Tomoya IMAI, Misako KOGA, Naoki SUNAGAWA, Kiyohiko IGARASHI, Kenji TAJIMA, Hiroaki TANAKA
International journal of microgravity science and application, 41, 3, 410302, 31 Jul. 2024, [Peer-reviewed]
English, Scientific journal, 34012600

Optimizing crystal transitions in low-temperature, low-concentration NaOH solutions to prepare cellulose I and II composite materials
Yuki Kugo, Takuya Isono, Masashi Fujiwara, Toshifumi Sato, Hirofumi Tani, Tomoki Erata, Kenji Tajima
Polymer Journal, Springer Science and Business Media LLC, 12 Jun. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Elucidating the structural changes of cellulose molecules and dynamics of Na ions during the crystal transition from cellulose I to II in low temperature and low concentration NaOH solution
Yuki Kugo, Satoshi Nomura, Takuya Isono, Shin-ichiro Sato, Masashi Fujiwara, Toshifumi Satoh, Hirofumi Tani, Tomoki Erata, Kenji Tajima
Carbohydrate Polymers, 332, 121907, 121907, Elsevier BV, May 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Organobase-Catalyzed Ring-Opening Copolymerization of Cyclic Anhydrides and Oxetanes: Establishment and Application in Block Copolymer Synthesis
Iyoka Ota, Ryota Suzuki, Yuta Mizukami, Xiaochao Xia, Kenji Tajima, Takuya Yamamoto, Feng Li, Takuya Isono, Toshifumi Satoh
Macromolecules, American Chemical Society (ACS), 11 Apr. 2024, [Peer-reviewed]
English, Scientific journal

Ultrasmall 3D network morphologies from biobased sugar–terpenoid hybrid block co-oligomers in the bulk and the thin film states
Chaehun Lee, Brian J. Ree, Kai Chen, Ryoya Komaki, Satoshi Katsuhara, Takuya Yamamoto, Redouane Borsali, Kenji Tajima, Hsin-Lung Chen, Toshifumi Satoh, Takuya Isono
Giant, 17, 100211, 100211, Elsevier BV, Mar. 2024, [Peer-reviewed]
English, Scientific journal

Acetyl Cellooligosaccharide-Based Block Copolymers for Toughening Cellulose Triacetate/Poly(ε-caprolactone) Biodegradable Blends
Satoshi Katsuhara, Yuki Tsuji, Naoki Sunagawa, Kiyohiko Igarashi, Kenji Takahashi, Takuya Yamamoto, Feng Li, Kenji Tajima, Takuya Isono, Toshifumi Satoh
ACS Sustainable Chemistry & Engineering, American Chemical Society (ACS), 14 Feb. 2024, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Crystallinity improvements in cellulose II after multiple posttreatment cycles with a dilute NaOH solution
Yuki Kugo, Satoshi Nomura, Takuya Isono, Masashi Fujiwara, Toshifumi Satoh, Hirofumi Tani, Tomoki Erata, Kenji Tajima
Polymer Journal, Springer Science and Business Media LLC, 13 Feb. 2024, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Chemically Recyclable Unnatural (1→6)-Polysaccharides from Cellulose-Derived Levoglucosenone and Dihydrolevoglucosenone
Yuta Mizukami, Yuto Kakehi, Feng Li, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
ACS Macro Letters, 252, 259, American Chemical Society (ACS), 09 Feb. 2024, [Peer-reviewed]
English, Scientific journal

Synthesis of Amino-Functionalized Polyester via Ring-Opening Alternating Copolymerization of Glycidylamines with Cyclic Anhydrides
Ryota Suzuki, Tianle Gao, Ayaka Sumi, Takuya Yamamoto, Kenji Tajima, Feng Li, Takuya Isono, Toshifumi Satoh
Polymer Chemistry, Royal Society of Chemistry (RSC), 2024
English, Scientific journal, Amino-functionalized polyesters (APEs) are a remarkable class of polymeric materials with a wide range of applications, e.g. as antibacterial materials, gene delivery carriers, and biodegradable plastics. However, the current APE...

Rotaxane Formation of Multicyclic Polydimethylsiloxane in a Silicone Network: A Step toward Constructing “Macro-Rotaxanes” from High-Molecular-Weight Axle and Wheel Components
Minami Ebe, Asuka Soga, Kaiyu Fujiwara, Brian J. Ree, Hironori Marubayashi, Katsumi Hagita, Atsushi Imasaki, Miru Baba, Takuya Yamamoto, Kenji Tajima, Tetsuo Deguchi, Hiroshi Jinnai, Takuya Isono, Toshifumi Satoh
Angewandte Chemie International Edition, 135, 35, e202304493, Wiley, Jul. 2023, [Peer-reviewed]
English, Scientific journal, Abstract

Rotaxanes consisting of a high‐molecular‐weight axle and wheel components (macro‐rotaxanes) have high structural freedom, and are attractive for soft‐material applications. However, their synthesis remains underexplored. Here, we investigated macro‐rotaxane formation by the topological trapping of multicyclic polydimethylsiloxanes (mc‐PDMSs) in silicone networks. mc‐PDMS with different numbers of cyclic units and ring sizes was synthesized by cyclopolymerization of a α,ω‐norbornenyl‐functionalized PDMS. Silicone networks were prepared in the presence of 10–60 wt % mc‐PDMS, and the trapping efficiency of mc‐PDMS was determined. In contrast to monocyclic PDMS, mc‐PDMSs with more cyclic units and larger ring sizes can be quantitatively trapped in the network as macro‐rotaxanes. The damping performance of a 60 wt % mc‐PDMS‐blended silicone network was evaluated, revealing a higher tan δ value than the bare PDMS network. Thus, macro‐rotaxanes are promising as non‐leaching additives for network polymers.

Effect of degree of substitution on the microphase separation and mechanical properties of cellooligosaccharide acetate-based elastomers
Katsuhara, S., Sunagawa, N., Igarashi, K., Takeuchi, Y., Takahashi, K., Yamamoto, T., Li, F., Tajima, K., Isono, T., Satoh, T.
Carbohydrate Polymers, 316, 120976, 120976, Elsevier {BV}, May 2023, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Bacterial Cellulose Nanofibers Modified with Quaternary Ammonium Salts for Antimicrobial Applications
Hiroyuki Kono, Yoichiro Sogame, Uugan-Erdene Purevdorj, Makoto Ogata, Kenji Tajima
ACS Applied Nano Materials, 6, 6, 4854, 4863, American Chemical Society (ACS), 02 Mar. 2023, [Peer-reviewed], [Last author]
English, Scientific journal

Molecular Weight-Dependent Oxidation and Optoelectronic Properties of Defect-Free Macrocyclic Poly(3-hexylthiophene)
Ryohei Sato, Atsuo Utagawa, Koji Fushimi, Feng Li, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Hiroshi Hirata, Yoshihiro Kikkawa, Takuya Yamamoto
Polymers, 15, 3, 666, 666, MDPI AG, 28 Jan. 2023, [Peer-reviewed]
English, Scientific journal, The redox behaviors of macrocyclic molecules with an entirely π-conjugated system are of interest due to their unique optical, electronic, and magnetic properties. In this study, defect-free cyclic P3HT with a degree of polymerization (DPn) from 14 to 43 was synthesized based on our previously established method, and its unique redox behaviors arising from the cyclic topology were investigated. Cyclic voltammetry (CV) showed that the HOMO level of cyclic P3HT decreases from –4.86 eV (14 mer) to –4.89 eV (43 mer), in contrast to the linear counterparts increasing from –4.94 eV (14 mer) to –4.91 eV (43 mer). During the CV measurement, linear P3HT suffered from electro-oxidation at the chain ends, while cyclic P3HT was stable. ESR and UV–Vis–NIR spectroscopy suggested that cyclic P3HT has stronger dicationic properties due to the interactions between the polarons. On the other hand, linear P3HT showed characteristics of polaron pairs with multiple isolated polarons. Moreover, the dicationic properties of cyclic P3HT were more pronounced for the smaller macrocycles.

Installation of the adamantyl group in polystyrene-block-poly(methyl methacrylate) via Friedel–Crafts alkylation to modulate the microphase-separated morphology and dimensions
Takuya Isono, Ema Baba, Shunma Tanaka, Ken Miyagi, Takahiro Dazai, Feng Li, Takuya Yamamoto, Kenji Tajima, Toshifumi Satoh
Polymer Chemistry, 14, 22, 2675, 2684, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed]
English, Scientific journal, We present the polystyrene block post-polymerization modification of PS-b-PMMA through Friedel–Crafts alkylation with adamantanols to modulate its microphase-separated morphology and dimensions.

Cyclopolymerization: A versatile approach to multicyclic polystyrene and polystyrene-containing multicyclic copolymer
Yamato Ebii, Yoshinobu Mato, Feng Li, Kenji Tajima, Takuya Yamamoto, Takuya Isono, Toshifumi Satoh
Polymer Chemistry, 14, 26, 3099, 3109, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed]
Scientific journal, Macromolecules with cyclic topologies have attracted significant attention because of their unique structures. Herein, we report the systematic synthesis of multicyclic polystyrene (mc-PS) via cyclopolymerization of α,ω-dinorbornenyl end-functionalized PS macromonomers...

Size Control and Enhanced Stability of Silver Nanoparticles by Cyclic Poly(ethylene glycol)
Wang, Y., Quinsaat, J.E.Q., Li, F., Isono, T., Tajima, K., Satoh, T., Sato, S.-I., Yamamoto, T.
Polymers, 14, 21, 4535, 4535, MDPI AG, 26 Oct. 2022, [Peer-reviewed]
English, Scientific journal, Silver nanoparticles (AgNPs) are used in a wide range of applications, and the size control and stability of the nanoparticles are crucial aspects in their applications. In the present study, cyclized poly(ethylene glycol) (c-PEG) with various molecular weights, along with linear PEG with hydroxy chain ends (HO–PEG–OH) and methoxy chain ends (MeO–PEG–OMe) were applied for the Tollens’ synthesis of AgNPs. The particle size was significantly affected by the topology and end groups of PEG. For example, the size determined by TEM was 40 ± 7 nm for HO–PEG5k–OH, 21 ± 4 nm for c-PEG5k, and 48 ± 9 nm for MeO–PEG5k–OMe when the molar ratio of PEG to AgNO3 (ω) was 44. The stability of AgNPs was also drastically improved by cyclization; the relative UV–Vis absorption intensity (A/A0 × 100%) at λmax to determine the proportion of persisting AgNPs in an aqueous NaCl solution (37.5 mM) was 58% for HO–PEG5k–OH, 80% for c-PEG5k, and 40% for MeO–PEG5k–OMe, despite the fact that AgNPs with c-PEG5k were much smaller than those with HO–PEG5k–OH and MeO–PEG5k–OMe.

Polyether/Polythioether Synthesis via Ring-Opening Polymerization of Epoxides and Episulfides Catalyzed by Alkali Metal Carboxylates
Tianle Gao, Xiaochao Xia, Kenji Tajima, Takuya Yamamoto, Takuya Isono, Toshifumi Satoh
Macromolecules, 55, 21, 9373, 9383, American Chemical Society (ACS), 21 Oct. 2022, [Peer-reviewed]
English, Scientific journal

Tailored Solid-State Carbohydrate Nanostructures Based on Star-Shaped Discrete Block Co-Oligomers
Takuya Isono, Ryoya Komaki, Nao Kawakami, Kai Chen, Hsin-Lung Chen, Chaehun Lee, Kazushige Suzuki, Brian J. Ree, Hiroaki Mamiya, Takuya Yamamoto, Redouane Borsali, Kenji Tajima, Toshifumi Satoh
Biomacromolecules, 23, 9, 3978, 3989, American Chemical Society (ACS), 12 Sep. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Carbohydrates are key building blocks for advanced functional materials owing to their biological functions and unique material properties. Here, we propose a star-shaped discrete block co-oligomer (BCO) platform to access carbohydrate nanostructures in bulk and thin-film states via the microphase separation of immiscible carbohydrate and hydrophobic blocks (maltooligosaccharides with 1-4 glucose units and solanesol, respectively). BCOs with various star-shaped architectures and saccharide volume fractions were synthesized using a modular approach. In the bulk, the BCOs self-assembled into common lamellar, cylindrical, and spherical carbohydrate microdomains as well as double gyroid, hexagonally perforated lamellar, and Fddd network morphologies with domain spacings of ∼7 nm. In thin films, long-range-ordered periodic carbohydrate microdomains were fabricated via spin coating. Such controlled spatial arrangements of functional carbohydrate moieties on the nanoscale have great application potential in biomedical and nanofabrication fields.

Synthesis of hyperbranched polyesters via the ring-opening alternating copolymerisation of epoxides with a cyclic anhydride having a carboxyl group
Ryota Suzuki, Xiaochao Xia, Tianle Gao, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Polymer Chemistry, 13, 38, 5469, 5477, Royal Society of Chemistry (RSC), Aug. 2022, [Peer-reviewed]
English, Scientific journal, Hyperbranched polyesters (HBPEs) are well-known interesting materials used in many fields.

Improving the mechanical properties of polycaprolactone using functionalized nanofibrillated bacterial cellulose with high dispersibility and long fiber length as a reinforcement material
Hashim, H.B., Emran, N.A.A.B., Isono, T., Katsuhara, S., Ninoyu, H., Matsushima, T., Yamamoto, T., Borsali, R., Satoh, T., Tajima, K.
Composites Part A: Applied Science and Manufacturing, 158, 106978, 106978, Elsevier {BV}, May 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 34012607

Fabrication of Ultrafine, Highly Ordered Nanostructures Using Carbohydrate-Inorganic Hybrid Block Copolymers
Taiki Nishimura, Satoshi Katsuhara, Chaehun Lee, Brian Ree, Redouane Borsali, Takuya Yamamoto, Kenji Tajima, Toshifumi Satoh, Takuya Isono
Nanomaterials, 12, 10, 1653, 1653, MDPI AG, May 2022, [Peer-reviewed]
Scientific journal, Block copolymers (BCPs) have garnered considerable interest due to their ability to form microphase-separated structures suitable for nanofabrication. For these applications, it is critical to achieve both sufficient etch selectivity and a small domain size. To meet both requirements concurrently, we propose the use of oligosaccharide and oligodimethylsiloxane as hydrophilic and etch-resistant hydrophobic inorganic blocks, respectively, to build up a novel BCP system, i.e., carbohydrate-inorganic hybrid BCP. The carbohydrate-inorganic hybrid BCPs were synthesized via a click reaction between oligodimethylsiloxane with an azido group at each chain end and propargyl-functionalized maltooligosaccharide (consisting of one, two, and three glucose units). In the bulk state, small-angle X-ray scattering revealed that these BCPs microphase separated into gyroid, asymmetric lamellar, and symmetric lamellar structures with domain-spacing ranging from 5.0 to 5.9 nm depending on the volume fraction. Additionally, we investigated microphase-separated structures in the thin film state and discovered that the BCP with the most asymmetric composition formed an ultrafine and highly oriented gyroid structure as well as in the bulk state. After reactive ion etching, the gyroid thin film was transformed into a nanoporous-structured gyroid SiO2 material, demonstrating the material’s promising potential as nanotemplates.

Topology and Sequence-Dependent Micellization and Phase Separation of Pluronic L35, L64, 10R5, and 17R4: Effects of Cyclization and the Chain Ends
Tomohisa Watanabe, Yubo Wang, Tomoko Ono, Satoru Chimura, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Daichi Ida, Takuya Yamamoto
Polymers, 14, 9, 1823, 1823, MDPI AG, 29 Apr. 2022, [Peer-reviewed]
English, Scientific journal, The topology effects of cyclization on thermal phase transition behaviors were investigated for a series of amphiphilic Pluronic copolymers of both hydrophilic–hydrophobic–hydrophilic and hydrophobic–hydrophilic–hydrophobic block sequences. The dye solubilization measurements revealed the lowered critical micelle temperatures (TCMT) along with the decreased micellization enthalpy (ΔHmic) and entropy (ΔSmic) for the cyclized species. Furthermore, the transmittance and dynamic light scattering (DLS) measurements indicated a block sequence-dependent effect on the clouding phenomena, where a profound decrease in cloud point (Tc) was only found for the copolymers with a hydrophilic–hydrophobic–hydrophilic block sequence. Thus, the effect of cyclization on these critical temperatures was manifested differently depending on its block sequence. Finally, a comparison of the linear hydroxy-terminated, methoxy-terminated, and cyclized species indicated the effect of cyclization to be unique from a simple elimination of the terminal hydrophilic moieties.

Physical characteristics and cell-adhesive properties of in vivo fabricated bacterial cellulose/hyaluronan nanocomposites
Takahama, R., Kato, H., Takayama, G., Tajima, K., Kondo, T.
Cellulose, 29, 6, 3239, 3251, Springer Science and Business Media {LLC}, 07 Mar. 2022, [Peer-reviewed]
English, Scientific journal

Reinforcing Poly (methyl methacrylate) with Bacterial Cellulose Nanofibers Chemically Modified with Methacryolyl Groups
Kono, H., Tsujisaki, H., Tajima, K.
Nanomaterials, 12, 3, 537, 537, MDPI AG, 04 Feb. 2022, [Peer-reviewed], [Last author]
Scientific journal, Nanofibrillated bacterial cellulose (NFBC), a type of cellulose nanofiber biosynthesized by Gluconacetobacter sp., has extremely long (i.e., high-aspect-ratio) fibers that are expected to be useful as nanofillers for fiber-reinforced composite resins. In this study, we investigated a composite of NFBC and poly(methyl methacrylate) (PMMA), a highly transparent resin, with the aim of improving the mechanical properties of the latter. The abundant hydroxyl groups on the NFBC surface were silylated using 3-(methacryloyloxy)propyltrimethoxysilane (MPTMS), a silane coupling agent bearing a methacryloyl group as the organic functional group. The surface-modified NFBC was homogeneously dispersed in chloroform, mixed with neat PMMA, and converted into PMMA composites using a simple solvent-casting method. The tensile strength and Young’s modulus of the composite increased by factors of 1.6 and 1.8, respectively, when only 0.10 wt% of the surface-modified NFBC was added, without sacrificing the maximum elongation rate. In addition, the composite maintained the high transparency of PMMA, highlighting that the addition of MPTMS-modified NFBC easily reinforce PMMA. Furthermore, interactions involving the organic functional groups of MPTMS were found to be very important for reinforcing PMMA.

Structural snapshot of a glycoside hydrolase family 8 endo-β-1,4-glucanase capturing the state after cleavage of the scissile bond
Takaaki Fujiwara, Ayumi Fujishima, Yui Nakamura, Kenji Tajima, Min Yao
Acta Crystallographica Section D Structural Biology, 78, 2, 228, 237, International Union of Crystallography (IUCr), 01 Feb. 2022, [Peer-reviewed]
English, Scientific journal, Bacterial cellulose (BC), which is produced by bacteria, is a biodegradable and biocompatible natural resource. Because of its remarkable physicochemical properties, BC has attracted attention for the development and manufacture of biomedical and industrial materials. In the BC production system, the enzyme endo-β-1,4-glucanase, which belongs to glycoside hydrolase family 8 (GH8), acts as a cleaner by trimming disordered cellulose fibers to produce high-quality BC. Understanding the molecular mechanism of the endo-β-1,4-glucanase would help in developing a reasonable biosynthesis of BC. Nevertheless, all of the steps in the reaction of this endo-β-1,4-glucanase are not clear. This study confirms the BC hydrolytic activity of the endo-β-1,4-glucanase from the BC-producing bacterium Enterobacter sp. CJF-002 (EbBcsZ) and reports crystal structures of EbBcsZ. Unlike in previously reported GH8 endo-β-1,4-glucanase structures, here the base catalyst was mutated (D242A) and the structure of this mutant bound to cellooligosaccharide [EbBcsZ(D242A)_CPT] was analyzed. The EbBcsZ(D242A)_CPT structure showed two cellooligosaccharides individually bound to the plus and minus subsites of EbBcsZ. The glucosyl unit in subsite −1 presented a distorted ⁵
S
₁ conformation, a novel snapshot of a state immediately after scissile-bond cleavage. In combination with previous studies, the reaction process of endo-β-1,4-glucanase is described and the β-1,4-glucan-trimming mechanism of EbBcsZ is proposed. The EbBcsZ(D242A)_CPT structure also showed an additional β-1,4-glucan binding site on the EbBcsZ surface, which may help to accept the substrate.

PEGylation of silver nanoparticles by physisorption of cyclic poly(ethylene glycol) for enhanced dispersion stability, antimicrobial activity, and cytotoxicity
Onyinyechukwu Justina Oziri, Yubo Wang, Tomohisa Watanabe, Shuya Uno, Masatoshi Maeki, Manabu Tokeshi, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Yutaka Miura, Takuya Yamamoto
Nanoscale Advances, 4, 2, 532, 545, Royal Society of Chemistry (RSC), 2022, [Peer-reviewed]
English, Scientific journal, Cyclic PEG without any chemical inhomogeneity was physiosorbed onto AgNPs to PEGylate and drastically enhance the dispersion stability against a physiological condition, white light, and high temperature, while its linear counterparts could not.

Cellulose-synthesizing machinery in bacteria
Tajima, K., Imai, T., Yui, T., Yao, M., Saxena, I.
Cellulose, 29, 5, 2755, 2777, Springer Science and Business Media {LLC}, 2022, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, 34000719

Topology-Dependent Interaction of Cyclic Poly(ethylene glycol) Complexed with Gold Nanoparticles against Bovine Serum Albumin for a Colorimetric Change
Onyinyechukwu Justina Oziri, Masatoshi Maeki, Manabu Tokeshi, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Takuya Yamamoto
Langmuir, 38, 17, 5286, 5295, American Chemical Society (ACS), 08 Dec. 2021, [Peer-reviewed], [International Magazine]
Japanese, Scientific journal, Unique physical and chemical properties arising from a polymer topology recently draw significant attention. In this study, cyclic poly(ethylene glycol) (c-PEG) was found to significantly interact with bovine serum albumin (BSA), suggested by nuclear magnetic resonance, dynamic light scattering, and fluorescence spectroscopy. On the other hand, linear HO-PEG-OH and MeO-PEG-OMe showed no affinity. Furthermore, a complex of gold nanoparticles and c-PEG (AuNPs/c-PEG) attracted BSA to form aggregates, and the red color of the AuNPs dispersion evidently disappeared, whereas ones with linear PEG or without PEG did not demonstrate such a phenomenon. The interactions among BSA, AuNPs, and PEG were investigated by changing the incubation time and concentration of the components by using UV-Vis and fluorescence spectroscopy.

Facile Post-Carboxymethylation of Cellulose Nanofiber Surfaces for Enhanced Water Dispersibility
Hiroyuki Kono, Eiki Tsukamoto, Kenji Tajima
ACS Omega, 6, 49, 34107, 34114, American Chemical Society (ACS), 01 Dec. 2021, [Peer-reviewed], [Last author]
English, Scientific journal

Suzuki–Miyaura Catalyst-Transfer Polycondensation of Triolborate-Type Carbazole Monomers
Saburo Kobayashi, Mayoh Ashiya, Takuya Yamamoto, Kenji Tajima, Yasunori Yamamoto, Takuya Isono, Toshifumi Satoh
Polymers, 13, 23, 4168, 4168, MDPI AG, 28 Nov. 2021, [Peer-reviewed]
English, Scientific journal, Herein, we report the Suzuki–Miyaura catalyst-transfer polycondensation (SCTP) of triolborate-type carbazole monomers, i.e., potassium 3-(6-bromo-9-(2-octyldodecyl)-9H-carbazole-2-yl)triolborate (M1) and potassium 2-(7-bromo-9-(2-octyldodecyl)-9H-carbazole-2-yl) triolborate (M2), as an efficient and versatile approach for precisely synthesizing poly[9-(2-octyldodecyl)-3,6-carbazole] (3,6-PCz) and poly[9-(2-octyldodecyl)-2,7-carbazole] (2,7-PCz), respectively. The SCTP of triolborate-type carbazole monomers was performed in a mixture of THF/H2O using an initiating system consisted of 4-iodobenzyl alcohol, Pd2(dba)3•CHCl3, and t-Bu3P. In the SCTP of M1, cyclic by-product formation was confirmed, as reported for the corresponding pinacolboronate-type monomer. By optimizing the reaction temperature and reaction time, we successfully synthesized linear end-functionalized 3,6-PCz for the first time. The SCTP of M2 proceeded with almost no side reaction, yielding 2,7-PCz with a functional initiator residue at the α-chain end. Kinetic and block copolymerization experiments demonstrated that the SCTP of M2 proceeded in a chain-growth and controlled/living polymerization manner. This is a novel study on the synthesis of 2,7-PCz via SCTP. By taking advantage of the well-controlled nature of this polymerization system, we demonstrated the synthesis of high-molecular-weight 2,7-PCzs (Mn = 5–38 kg mol−1) with a relatively narrow ÐM (1.35–1.48). Furthermore, we successfully synthesized fluorene/carbazole copolymers as well as 2,7-PCz-containing diblock copolymers, demonstrating the versatility of the present polymerization system as a novel synthetic strategy for well-defined polycarbazole-based materials.

Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter
Ryo Takahama, Honami Kato, Kenji Tajima, Satomi Tagawa, Tetsuo Kondo
Biomacromolecules, 22, 11, 4709, 4719, American Chemical Society (ACS), 08 Nov. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms. Their hierarchically regulated production systems show potential for designing nanocomposite fabrication using engineered microorganisms. This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC). The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis. Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii. The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites. This genetic engineering approach facilitates the use of G. hansenii for designing integrated cellulose-based nanomaterials.

Densely Arrayed Cage-Shaped Polymer Topologies Synthesized via Cyclopolymerization of Star-Shaped Macromonomers
Yoshinobu Mato, Maho Sudo, Hironori Marubayashi, Brian J. Ree, Kenji Tajima, Takuya Yamamoto, Hiroshi Jinnai, Takuya Isono, Toshifumi Satoh
Macromolecules, 54, 19, 9079, 9090, American Chemical Society ({ACS}), 12 Oct. 2021, [Peer-reviewed]
English, Scientific journal

Enhanced Self-Assembly and Mechanical Properties of Cellulose-Based Triblock Copolymers: Comparisons with Amylose-Based Triblock Copolymers
Satoshi Katsuhara, Yasuko Takagi, Naoki Sunagawa, Kiyohiko Igarashi, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
ACS Sustainable Chemistry & Engineering, 9, 29, 9779, 9788, American Chemical Society (ACS), 08 Jul. 2021, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Herein, we compared the microphase-separation behavior and mechanical properties of cellulose- and amylose-based block copolymers (BCPs). Various cellooligosaccharide triacetate-b-poly(δ-decanolactone)-b-cellooligosaccharide triacetates (AcCeln-b-PDL-b-AcCelns), which are cellulose-based ABA-type BCPs, with PDL molecular weights of approximately 5, 10, and 20 kg mol-1and PDL volume fractions of 0.65, 0.77, and 0.87, were synthesized from α,ω-diazido-end-functionalized PDLs and propargyl-end-functionalized cellooligosaccharide triacetates via click chemistry. We adopted the cellodextrin-phosphorylase-mediated oligomerization of α-glucose-1-phosphase in the presence of a propargyl-end-functionalized cellobiose primer to synthesize the functional cellooligosaccharide segment. The maltooligosaccharide triacetate-b-poly(δ-decanolactone)-b-maltooligosaccharide triacetate (AcMaln-b-PDL-b-AcMalns) amylose counterparts were also synthesized in a similar manner. Small-angle X-ray scattering experiments and atomic force microscopy revealed that AcCeln-b-PDL-b-AcCelns are more likely to microphase-separate into ordered nanostructures compared to AcMaln-b-PDL-b-AcMalns, despite their comparable chemical compositions and molecular weights. Furthermore, AcCeln-b-PDL-b-AcCelns exhibited significantly superior mechanical performance compared to their amylose counterparts under tensile testing, with Young’s modulus and stress at break of AcCeln-b-PDL10k-b-AcCelnbeing 2.3 and 1.8 times higher, respectively, than those of AcMaln-b-PDL10k-b-AcMaln. The enhanced microphase-separation and mechanical properties of AcCeln-b-PDL-b-AcCelns were found to be attributable to the stiffness and crystalline nature of the AcCelnsegments. These results demonstrate the advantages of using cellulose derivatives to synthesize novel biofunctional materials.

One‐Shot Intrablock Cross‐Linking of Linear Diblock Copolymer to Realize Janus‐Shaped Single‐Chain Nanoparticles
Kodai Watanabe, Noya Kaizawa, Brian J. Ree, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Angewandte Chemie International Edition, 60, 33, 18122, 18128, Wiley, 08 Jul. 2021, [Peer-reviewed]
English, Scientific journal

Doxorubicin Embedded into Nanofibrillated Bacterial Cellulose (NFBC) Produces a Promising Therapeutic Outcome for Peritoneally Metastatic Gastric Cancer in Mice Models via Intraperitoneal Direct Injection
Hidenori Ando, Takashi Mochizuki, Amr S. Abu Lila, Shunsuke Akagi, Kenji Tajima, Kenji Fujita, Taro Shimizu, Yu Ishima, Tokuo Matsushima, Takatomo Kusano, Tatsuhiro Ishida
Nanomaterials, 11, 7, 1697, 1697, MDPI AG, 28 Jun. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Natural materials such as bacterial cellulose are gaining interest for their use as drug-delivery vehicles. Herein, the utility of nanofibrillated bacterial cellulose (NFBC), which is produced by culturing a cellulose-producing bacterium (Gluconacetobacter intermedius NEDO-01) in a medium supplemented with carboxymethylcellulose (CMC) that is referred to as CM-NFBC, is described. Recently, we demonstrated that intraperitoneal administration of paclitaxel (PTX)-containing CM-NFBC efficiently suppressed tumor growth in a peritoneally disseminated cancer xenograft model. In this study, to confirm the applicability of NFBC in cancer therapy, a chemotherapeutic agent, doxorubicin (DXR), embedded into CM-NFBC, was examined for its efficiency to treat a peritoneally disseminated gastric cancer via intraperitoneal administration. DXR was efficiently embedded into CM-NFBC (DXR/CM-NFBC). In an in vitro release experiment, 79.5% of DXR was released linearly into the peritoneal wash fluid over a period of 24 h. In the peritoneally disseminated gastric cancer xenograft model, intraperitoneal administration of DXR/CM-NFBC induced superior tumor growth inhibition (TGI = 85.5%) by day 35 post-tumor inoculation, compared to free DXR (TGI = 62.4%). In addition, compared with free DXR, the severe side effects that cause body weight loss were lessened via treatment with DXR/CM-NFBC. These results support the feasibility of CM-NFBC as a drug-delivery vehicle for various anticancer agents. This approach may lead to improved therapeutic outcomes for the treatment of intraperitoneally disseminated cancers.

Cyclization of PEG and Pluronic Surfactants and the Effects of the Topology on Their Interfacial Activity
Tomohisa Watanabe, Satoru Chimura, Yubo Wang, Tomoko Ono, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Daichi Ida, Takuya Yamamoto
Langmuir, 37, 23, 6974, 6984, American Chemical Society (ACS), 28 May 2021, [Peer-reviewed]
English, Scientific journal

Therapeutic efficacy of a paclitaxel-loaded nanofibrillated bacterial cellulose (PTX/NFBC) formulation in a peritoneally disseminated gastric cancer xenograft model
Shunsuke Akagi, Hidenori Ando, Kenji Fujita, Taro Shimizu, Yu Ishima, Kenji Tajima, Tokuo Matsushima, Takatomo Kusano, Tatsuhiro Ishida
International Journal of Biological Macromolecules, 174, 494, 501, Elsevier BV, Mar. 2021, [Peer-reviewed]
English, Scientific journal

Stretchable OFET Memories: Tuning the Morphology and the Charge-Trapping Ability of Conjugated Block Copolymers through Soft Segment Branching.
Li-Che Hsu, Takuya Isono, Yan-Cheng Lin, Saburo Kobayashi, Yun-Chi Chiang, Dai-Hua Jiang, Chih-Chien Hung, Ender Ercan, Wei-Chen Yang, Hui-Ching Hsieh, Kenji Tajima, Toshifumi Satoh, Wen-Chang Chen
ACS applied materials & interfaces, 13, 2, 2932, 2943, 20 Jan. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, The mechanical properties and structural design flexibility of charge-trapping polymer electrets have led to their widespread use in organic field-effect transistor (OFET) memories. For example, in the electrets of polyfluorene-based conjugated/insulating block copolymers (BCPs), the confined fiberlike polyfluorene nanostructures in the insulating polymer matrix act as effective hole-trapping sites, leading to controllable memory performance through the design of BCPs. However, few studies have reported intrinsically stretchable charge-trapping materials and their memory device applications, and a practical method to correlate the thin-film morphology of BCP electrets with their charge-trapping ability has not yet been developed. In this study, a series of new conjugated/insulating BCPs, poly(9,9-di-n-hexyl-2,7-fluorene)-block-poly(δ-decanolactone)s (PF-b-PDL
x
, x = 1-3), as stretchable hole-trapping materials are reported. The linear and branched PDL blocks with comparable molecular weights were used to investigate the effect of polymer architecture on morphology and device performance. Moreover, the coverage area of the polyfluorene nanofibers on the BCP films was extracted from atomic force microscopy images, which can be correlated with the trapping density of the polymer electrets. The branched PDL segments not only improve stretchability but also tailor crystallinity and phase separation of the BCPs, thus increasing their charge-trapping ability. The OFET memory device with PF-b-PDL3 as the electret layer exhibited the largest memory window (102 V) and could retain its performance at up to 100% strain. This research highlights the importance of the BCP design for developing stretchable charge-trapping materials.

Molecular Dynamics Simulation of Cellulose Synthase Subunit D Octamer with Cellulose Chains from Acetic Acid Bacteria: Insight into Dynamic Behaviors and Thermodynamics on Substrate Recognition
Takuya Uto, Yuki Ikeda, Naoki Sunagawa, Kenji Tajima, Min Yao, Toshifumi Yui
Journal of Chemical Theory and Computation, 17, 1, 488, 496, American Chemical Society (ACS), 31 Dec. 2020, [Peer-reviewed]
English, Scientific journal

Enhanced dispersion stability of gold nanoparticles by the physisorption of cyclic poly(ethylene glycol)
Yubo Wang, Jose Enrico Q. Quinsaat, Tomoko Ono, Masatoshi Maeki, Manabu Tokeshi, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Shin-ichiro Sato, Yutaka Miura, Takuya Yamamoto
Nature Communications, 11, 1, Springer Science and Business Media LLC, 30 Nov. 2020, [Peer-reviewed]
English, Scientific journal, Abstract

Nano-sized metal particles are attracting much interest in industrial and biomedical applications due to the recent progress and development of nanotechnology, and the surface-modifications by appropriate polymers are key techniques to stably express their characteristics. Herein, we applied cyclic poly(ethylene glycol) (c-PEG), having no chemical inhomogeneity, to provide a polymer topology-dependent stabilization for the surface-modification of gold nanoparticles (AuNPs) through physisorption. By simply mixing c-PEG, but not linear counterparts, enables AuNPs to maintain dispersibility through freezing, lyophilization, or heating. Surprisingly, c-PEG endowed AuNPs with even better dispersion stability than thiolated PEG (HS–PEG–OMe). The stronger affinity of c-PEG was confirmed by DLS, ζ-potential, and FT-IR. Furthermore, the c-PEG system exhibited prolonged blood circulation and enhanced tumor accumulation in mice. Our data suggests that c-PEG induces physisorption on AuNPs, supplying sufficient stability toward bio-medical applications, and would be an alternative approach to the gold–sulfur chemisorption.

Nanofibrillated Bacterial Cellulose Modified with (3-Aminopropyl)trimethoxysilane under Aqueous Conditions: Applications to Poly(methyl methacrylate) Fiber-Reinforced Nanocomposites
Kono, H., Uno, T., Tsujisaki, H., Matsushima, T., Tajima, K.
ACS Omega, 5, 45, 29561, 29569, American Chemical Society ({ACS}), 17 Nov. 2020, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, 13483839

Suzuki–Miyaura Catalyst-Transfer Polycondensation of Triolborate-Type Fluorene Monomer: Toward Rapid Access to Polyfluorene-Containing Block and Graft Copolymers from Various Macroinitiators
Saburo Kobayashi, Kaiyu Fujiwara, Dai-Hua Jiang, Takuya Yamamoto, Kenji Tajima, Yasunori Yamamoto, Takuya Isono, Toshifumi Satoh
Polymer Chemistry, 11, 42, 6832, 6839, Royal Society of Chemistry (RSC), 09 Oct. 2020, [Peer-reviewed]
Scientific journal,

In this study, we demonstrated that the Suzuki–Miyaura catalyst transfer polycondensation (SCTP) of the triolborate-type fluorene monomer, viz. potassium 2-(7-bromo-9,9-dihexyl-9H-fluorene-2-yl)triolborate, can be an efficient and versatile approach to the precise...

Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures
Takuya Isono, Ryoya Komaki, Chaehun Lee, Nao Kawakami, Brian J. Ree, Kodai Watanabe, Kohei Yoshida, Hiroaki Mamiya, Takuya Yamamoto, Redouane Borsali, Kenji Tajima, Toshifumi Satoh
Communications Chemistry, 3, 1, Springer Science and Business Media LLC, 09 Oct. 2020, [Peer-reviewed]
Scientific journal,
Discrete block co-oligomers (BCOs) are gaining considerable attention due to their potential to form highly ordered ultrasmall nanostructures suitable for lithographic templates. However, laborious synthetic routes present a major hurdle to the practical application. Herein, we report a readily available discrete BCO system that is capable of forming various self-assembled nanostructures with ultrasmall periodicity. Click coupling of propargyl-functionalized sugars (containing 1–7 glucose units) and azido-functionalized terpenoids (containing 3, 4, and 9 isoprene units) afforded the discrete and monodisperse BCOs with a desired total degree of polymerization and block ratio. These BCOs microphase separated into lamellar, gyroid, and cylindrical morphologies with the domain spacing (d) of 4.2–7.5 nm. Considering easy synthesis and rich phase behavior, presented BCO systems could be highly promising for application to diverse ~4-nm nanofabrications.

An organocatalytic ring-opening polymerization approach to highly alternating copolymers of lactic acid and glycolic acid
Kaoru Takojima, Hiroshi Makino, Tatsuya Saito, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Polymer Chemistry, 11, 39, 6365, 6373, Royal Society of Chemistry (RSC), 07 Sep. 2020, [Peer-reviewed]
English, Scientific journal,

Highly regioselective ring-opening polymerization of optically active methylglycolides was achived using P₂-t-Bu/alcohol system to produce alternating copolymer of lactic acid and glycolic acid.

Nanofibrillated Bacterial Cellulose Surface Modified with Methyltrimethoxysilane for Fiber-Reinforced Composites
Kono, H., Uno, T., Tsujisaki, H., Anai, H., Kishimoto, R., Matsushima, T., Tajima, K.
ACS Applied Nano Materials, 3, 8, 8232, 8241, American Chemical Society ({ACS}), 14 Aug. 2020, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

Highly asymmetric lamellar nanostructures from nanoparticle-linear hybrid block copolymers.
Kodai Watanabe, Satoshi Katsuhara, Hiroaki Mamiya, Yukihiko Kawamura, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Nanoscale, 12, 31, 16526, 16534, Royal Society of Chemistry (RSC), 13 Aug. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, The highly asymmetric lamellar (A-LAM) nanostructure is one of the most important template geometries for block copolymer (BCP) lithography. However, A-LAM is unattainable from conventional BCPs, and there is no general molecular design strategy for A-LAM-forming BCP. Herein, a nanoparticle-linear hybrid BCP system is reported, which is designed based on the intramolecular crosslinking technique, as a remarkably effective platform to obtain the A-LAM morphology. The hybrid BCPs consisting of polystyrene single-chain nanoparticles and linear polylactide segments show a remarkable capability to form the A-LAM morphology in bulk, where a maximum width ratio of 4.1 between the two domains is obtained. This unusual phase behavior is attributed to the bulky and rigid characteristics of the nanoparticle block. Furthermore, the thin films of these hybrid BCPs show perpendicularly oriented A-LAM morphology on a chemically modified Si substrate, allowing promising application in the fabrication of asymmetric line-and-space nanopatterns.

Programmed folding into spiro-multicyclic polymer topologies from linear and star-shaped chains
Yoshinobu Mato, Kohei Honda, Brian J. Ree, Kenji Tajima, Takuya Yamamoto, Tetsuo Deguchi, Takuya Isono, Toshifumi Satoh
Communications Chemistry, 3, 1, Springer Science and Business Media LLC, 04 Aug. 2020, [Peer-reviewed]
Scientific journal

Sweet Pluronic poly(propylene oxide)-b-oligosaccharide block copolymer systems: Toward sub-4 nm thin-film nanopattern resolution
Mumtaz, M., Takagi, Y., Mamiya, H., Tajima, K., Bouilhac, C., Isono, T., Satoh, T., Borsali, R.
European Polymer Journal, 134, 109831, 109831, Elsevier BV, Jul. 2020, [Peer-reviewed]
English, Scientific journal

Carbohydrates as Hard Segments for Sustainable Elastomers: Carbohydrates Direct the Self-Assembly and Mechanical Properties of Fully Bio-Based Block Copolymers
Takuya Isono, Saki Nakahira, Hui-Ching Hsieh, Satoshi Katsuhara, Hiroaki Mamiya, Takuya Yamamoto, Wen-Chang Chen, Redouane Borsali, Kenji Tajima, Toshifumi Satoh
Macromolecules, 53, 13, 5408, 5417, American Chemical Society (ACS), 16 Jun. 2020, [Peer-reviewed]
English, Scientific journal

Detailed Structural Analyses of Nanofibrillated Bacterial Cellulose and Its Application as Binder Material for a Display Device.
Kenji Tajima, Koutaro Tahara, Junya Ohba, Ryo Kusumoto, Ryota Kose, Hiroyuki Kono, Tokuo Matsushima, Koji Fushimi, Takuya Isono, Takuya Yamamoto, Toshifumi Satoh
Biomacromolecules, 21, 2, 581, 588, 10 Feb. 2020, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, Nanofibrillated bacterial cellulose (NFBC) is produced by culturing a cellulose-producing bacterium under agitated aerobic conditions in a carboxymethylcellulose (CMC)-supplemented medium. Detailed structural analyses revealed that NFBC fiber widths varied with the degree of substitution of the CMC used, and zeta potential values decreased with the increment of CMC concentration in the medium. Transmission electron microscopy observation after immunostaining demonstrated that CMC molecules were present on the NFBC microfibril surfaces. We tested NFBC for utility as a binder for a display device that uses electrochromic (EC) material. Introduction of a quaternary ammonium group into the EC molecules enhanced their interactions with the negatively charged NFBC microfibrils. A casting process homogeneously adsorbed the EC molecules onto the surface of a transparent electrode with NFBC. A homogeneous color change was successfully observed upon applying an electric field, suggesting that NFBC could be used as a binder material for uniform surface adsorption.

Facile synthesis of poly(trimethylene carbonate) by alkali metal carboxylate-catalyzed ring-opening polymerization
Kaoru Takojima, Tatsuya Saito, Cedric Vevert, Viko Ladelta, Panayiotis Bilalis, Jun Watanabe, Shintaro Hatanaka, Takashi Konno, Takuya Yamamoto, Kenji Tajima, Nikos Hadjichristidis, Takuya Isono, Toshifumi Satoh
Polymer Journal, 52, 1, 103, 110, Springer Science and Business Media LLC, Jan. 2020, [Peer-reviewed]
Scientific journal

Metallopolymer-block-oligosaccharide for sub-10 nm microphase separation
Satoshi Katsuhara, Hiroaki Mamiya, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Polymer Chemistry, 11, 17, 2995, 3002, Royal Society of Chemistry (RSC), 2020, [Peer-reviewed]
English, Scientific journal,

The novel high-χ BCPs comprising poly(vinyl ferrocene) and oligosaccharides formed hexagonal cylinder morphology with d values of ∼8 nm. Lamellar morphology with d values of ∼9 nm was also realized by mixing these polymers and glucose.

Structural and rheological characterization of bacterial cellulose gels obtained from Gluconacetobacter genus
Numata, Y., Kono, H., Mori, A., Kishimoto, R., Tajima, K.
Food Hydrocolloids, 92, 233, 239, Jul. 2019, [Peer-reviewed], [Last author]
English, Scientific journal

Installing a functional group into the inactive ω-chain end of PMMA and PS-: B -PMMA by terminal-selective transesterification
Yoshida, K., Yamamoto, T., Tajima, K., Isono, T., Satoh, T.
Polymer Chemistry, 10, 24, 3390, 3398, Jun. 2019, [Peer-reviewed]
English, Scientific journal,

Various functional groups were incorporated into the inherently inactive ω-chain end of polymethacrylate-based polymers by terminal-selective transesterification.

Trimethyl Glycine as an Environmentally Benign and Biocompatible Organocatalyst for Ring-Opening Polymerization of Cyclic Carbonate
Tatsuya Saito, Kaoru Takojima, Takafumi Oyama, Shintaro Hatanaka, Takashi Konno, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
ACS Sustainable Chemistry & Engineering, 7, 9, 8868, 8875, American Chemical Society (ACS), 06 May 2019, [Peer-reviewed]
English, Scientific journal

Biodegradable Compatibilizers for Poly(hydroxyalkanoate)/Poly(ε-caprolactone) Blends through Click Reactions with End-Functionalized Microbial Poly(hydroxyalkanoate)s
Takafumi Oyama, Shingo Kobayashi, Tetsuo Okura, Shunsuke Sato, Kenji Tajima, Takuya Isono, Toshifumi Satoh
ACS Sustainable Chemistry & Engineering, 7, 8, 7969, 7978, American Chemical Society (ACS), 15 Apr. 2019, [Peer-reviewed]
English, Scientific journal

Downsizing feature of microphase-separated structures via intramolecular crosslinking of block copolymers.
Kodai Watanabe, Satoshi Katsuhara, Hiroaki Mamiya, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Chemical science, 10, 11, 3330, 3339, 21 Mar. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, A novel strategy for downsizing the feature of microphase-separated structures was developed via the intramolecular crosslinking reaction of block copolymers (BCPs) without changing the molecular weight. A series of BCPs consisting of poly[styrene-st-(p-3-butenyl styrene)] and poly(rac-lactide) (SBS-LA) was subjected to Ru-catalyzed olefin metathesis under highly diluted conditions to produce intramolecularly crosslinked BCPs (SBS(cl)-LAs). Small-angle X-ray scattering measurement and transmission electron microscopy observation of the SBS(cl)-LAs revealed feature size reduction in lamellar (LAM) and hexagonally close-packed cylinder (HEX) structures in the bulk state, which was surely due to the restricted chain dimensions of the intramolecularly crosslinked SBS block. Notably, the degree of size reduction was controllable by varying the crosslink density, with a maximum decrease of 22% in the LAM spacing. In addition, we successfully observed the downsizing of the HEX structure in the thin film state using atomic force microscopy, indicating the applicability of the present methodology to next-generation lithography technology.

Microphase separation of carbohydrate-based star-block copolymers with sub-10 nm periodicity
Isono, T., Kawakami, N., Watanabe, K., Yoshida, K., Otsuka, I., Mamiya, H., Ito, H., Yamamoto, T., Tajima, K., Borsali, R., Satoh, T.
Polymer Chemistry, 10, 9, 1119, 1129, Mar. 2019, [Peer-reviewed]
English, Scientific journal,

Star-block copolymers consisting of polycaprolactone and maltotriose segments with three, four, and six arms were synthesized to achieve sub-10 nm microphase-separated structures.

A versatile synthetic strategy for macromolecular cages: intramolecular consecutive cyclization of star-shaped polymers.
Yoshinobu Mato, Kohei Honda, Kenji Tajima, Takuya Yamamoto, Takuya Isono, Toshifumi Satoh
Chemical science, 10, 2, 440, 446, 14 Jan. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Cage-shaped polymers, or "macromolecular cages", are of great interest as the macromolecular analogues of molecular cages because of their various potential applications in supramolecular chemistry and materials science. However, the systematic synthesis of macromolecular cages remains a great challenge. Herein, we describe a robust and versatile synthetic strategy for macromolecular cages with defined arm numbers and sizes based on the intramolecular consecutive cyclization of highly reactive norbornene groups attached to each end of the arms of a star-shaped polymer precursor. The cyclizations of three-, four-, six-, and eight-armed star-shaped poly(ε-caprolactone)s (PCLs) bearing a norbornenyl group at each arm terminus were effected with Grubbs' third generation catalyst at high dilution. 1H NMR, SEC, and MALDI-TOF MS analyses revealed that the reaction proceeded to produce the desired macromolecular cages with sufficient purity. The molecular sizes of the macromolecular cages were controlled by simply changing the molecular weight of the star-shaped polymer precursors. Systematic investigation of the structure-property relationships confirmed that the macromolecular cages adopt a much more compact conformation, in both the solution and bulk states, as compared to their linear and star-shaped counterparts. This synthetic approach marks a significant advance in the synthesis of complex macromolecular architectures and provides a platform for novel applications using cage-shaped molecules with polymer frameworks.

Chain-End Functionalization with a Saccharide for 10 nm Microphase Separation: "classical" PS- b-PMMA versus PS- b-PMMA-Saccharide
Yoshida, K., Tanaka, S., Yamamoto, T., Tajima, K., Borsali, R., Isono, T., Satoh, T.
Macromolecules, 51, 21, 8870, 8877, 13 Nov. 2018, [Peer-reviewed]
English, Scientific journal

Synthesis, Isolation, and Properties of All Head-to-Tail Cyclic Poly(3-hexylthiophene): Fully Delocalized Exciton over the Defect-Free Ring Polymer
Takuya Yamamoto, Masaaki Hosokawa, Minato Nakamura, Shin-ichiro Sato, Takuya Isono, Kenji Tajima, Toshifumi Satoh, Masamichi Sato, Yasuyuki Tezuka, Akinori Saeki, Yoshihiro Kikkawa
Macromolecules, 51, 22, 9284, 9293, American Chemical Society (ACS), Nov. 2018, [Peer-reviewed]
English, Scientific journal

Facile and Efficient Modification of Polystyrene- block-poly(methyl methacrylate) for Achieving Sub-10 nm Feature Size
Yoshida, K., Tian, L., Miyagi, K., Yamazaki, A., Mamiya, H., Yamamoto, T., Tajima, K., Isono, T., Satoh, T.
Macromolecules, 51, 20, 8064, 8072, 23 Oct. 2018, [Peer-reviewed]
English, Scientific journal

Synthesis of μ-ABC Tricyclic Miktoarm Star Polymer via Intramolecular Click Cyclization.
Tomoki Shingu, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Polymers, 10, 8, 877, 06 Aug. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Cyclic polymers exhibit unique physical and chemical properties because of the restricted chain mobility and absence of chain ends. Although many types of homopolymers and diblock copolymers possessing cyclic architectures have been synthesized to date, there are relatively few reports of cyclic triblock terpolymers because of their synthetic difficulties. In this study, a novel synthetic approach for μ-ABC tricyclic miktoarm star polymers involving t-Bu-P₄-catalyzed ring-opening polymerization (ROP) of glycidyl ethers and intramolecular copper-catalyzed azido-alkyne cycloaddition (CuAAC) was developed. First, the t-Bu-P₄-catalyzed ROP of decyl glycidyl ether, dec-9-enyl glycidyl ether, and 2-(2-(2-methoxyethoxy) ethoxy) ethyl glycidyl ether with the aid of functional initiators and terminators was employed for the preparation of a clickable linear triblock terpolymer precursor possessing three azido and three ethynyl groups at the selected positions. Next, the intramolecular CuAAC of the linear precursor successfully produced the well-defined tricyclic triblock terpolymer with narrow dispersity in a reasonable yield. The present strategy is useful for synthesizing model polymers for studying the topological effects on the triblock terpolymer self-assembly.

Multicyclic Polymer Synthesis through Controlled/Living Cyclopolymerization of α,ω-Dinorbornenyl-Functionalized Macromonomers
Takuya Isono, Tetsuya Sasamori, Kohei Honda, Yoshinobu Mato, Takuya Yamamoto, Kenji Tajima, Toshifumi Satoh
Macromolecules, 51, 10, 3855, 3864, American Chemical Society, 22 May 2018, [Peer-reviewed]
English, Scientific journal, A novel synthesis of multicyclic polymers that feature ultradense arrays of cyclic polymer units has been developed by exploiting the cyclopolymerization of α,ω-norbornenyl end-functionalized macromonomers mediated by the Grubbs third-generation catalyst (G3). Owing to the living polymerization nature, the number of cyclic repeating units in these multicyclic polymers was controlled to be between 1 and approximately 70 by varying the initial macromonomer-to-G3 ratio. The ring size was also tuned by choosing the molecular weight of the macromonomer
in this way we successfully prepared multicyclic polymers that possess cyclic repeating units composed of up to about 500 atoms, which by far exceeds those prepared to date by cyclopolymerization. Specifically, cyclopolymerizations of α,ω-norbornenyl end-functionalized poly(l-lactide)s (PLLAs) proceeded homogeneously under highly dilute conditions (∼0.1 mM in CH2Cl2) to give multicyclic polymers that feature cyclic PLLA repeating units on the polynorbornene backbone. The cyclic product architectures were confirmed not only by structural characterization based on NMR, MALDI-TOF MS, and SEC analyses but also by comparing their glass transition temperatures, viscosities, and hydrodynamic radii with their acyclic counterparts. The cyclopolymerization strategy was applicable to a variety of α,ω-norbornenyl end-functionalized macromonomers, such as poly(ϵ-caprolactone), poly(ethylene glycol) (PEG), poly(tetrahydrofuran), and PLLA-b-PEG-b-PLLA. The successful statistical and block cyclocopolymerizations of the PLLA and PEG macromonomers gave amphiphilic multicyclic copolymers.

Alkali Metal Carboxylate as an Efficient and Simple Catalyst for Ring-Opening Polymerization of Cyclic Esters
Tatsuya Saito, Yusuke Aizawa, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
Macromolecules, 51, 3, 689, 696, American Chemical Society, 13 Feb. 2018, [Peer-reviewed]
English, Scientific journal, Alkali metal carboxylates were discovered as efficient and simple catalysts for the ring-opening polymerization of cyclic esters that are alternatives to conventional metal-based catalysts and organocatalysts. In our system using an alcohol initiator and this simple catalyst, biodegradable and biocompatible aliphatic polyesters, such as poly(lactide), poly(e-caprolactone), poly(δ-valerolactone), and poly(trimethylene carbonate), were obtained with predictive molecular weights ranging from 3500 to 22-600 and narrow dispersities. A kinetic experiment for the ROP of l-lactide confirmed the controlled/living nature of the present ROP system, which allowed the precise synthesis of end-functionalized polyesters as well as multihydroxyl-containing polyesters, including α,hydroxy telechelic and star-shaped polyesters. Furthermore, a block copolymer containing the poly(l-lactide) segment was successfully synthesized using a macroinitiator possessing a hydroxyl group at the chain end. The tunability of the alkali metal carboxylates by the appropriate choice of the alkyl moiety and countercation enables not only control of the polymerization behavior but also expansion of the scope of the applicable monomers. Given the low cost, easy handling, and low toxicity of the alkali metal carboxylates, the present ROP system can be highly promising for both laboratory- and industrial-scale polyester productions.

Highly Ordered Cylinder Morphologies with 10 nm Scale Periodicity in Biomass-Based Block Copolymers
Takuya Isono, Brian J. Ree, Kenji Tajima, Redouane Borsali, Toshifumi Satoh
Macromolecules, 51, 2, 428, 437, American Chemical Society, 23 Jan. 2018, [Peer-reviewed]
English, Scientific journal, Microphase-separated structures of block copolymers (BCPs) have attracted considerable attention for their potential application in the bottom-up fabrication of 10 nm scale nanostructured materials. To realize sustainable development within this field, the creation of novel BCP materials from renewable biomass resources is of fundamental interest. Thus, we herein focused on maltoheptaose-b-poly(δ-decanolactone)-b-maltoheptaose (MH-b-PDL-b-MH) as a sustainable alternative for nanostructure-forming BCPs, in which both constitutional blocks can be derived from renewable biomass resources, in the case, δ-decanolactone and amylose. Well-defined MH-b-PDL-b-MHs with varying PDL lengths were synthesized through a combination of controlled/living ring-opening polymerization and the click reaction. The prepared MH-b-PDL-b-MHs successfully self-assembled into highly ordered hexagonal cylindrical structures with a domain-spacing of ∼12-14 nm in both the bulk and thin film states. Interestingly, the as-cast thin films of MH-b-PDL-b-MHs (with PDL lengths of 9K and 13K) form horizontal cylinders, with thermal annealing (180 °C, 30 min) resulting in a drastic change in the domain orientation from horizontal to vertical. Thus, the results presented herein demonstrated that the combination of oligosaccharides and biomass-derived hydrophobic polymers appears promising for the sustainable development of nanotechnology and related fields.

Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C.
Shingo Nojima, Ayumi Fujishima, Koji Kato, Kayoko Ohuchi, Nobutaka Shimizu, Kento Yonezawa, Kenji Tajima, Min Yao
Scientific reports, 7, 1, 13018, 13018, NATURE PUBLISHING GROUP, 12 Oct. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal, Bacterial cellulose (BC) is synthesized and exported through the cell membrane via a large protein complex (terminal complex) that consists of three or four subunits. BcsC is a little-studied subunit considered to export BC to the extracellular matrix. It is predicted to have two domains: a tetratrico peptide repeat (TPR) domain and a β-barrelled outer membrane domain. Here we report the crystal structure of the N-terminal part of BcsC-TPR domain (Asp24-Arg272) derived from Enterobacter CJF-002. Unlike most TPR-containing proteins which have continuous TPR motifs, this structure has an extra α-helix between two clusters of TPR motifs. Five independent molecules in the crystal had three different conformations that varied at the hinge of the inserted α-helix. Such structural feature indicates that the inserted α-helix confers flexibility to the chain and changes the direction of the TPR super-helix, which was also suggested by structural analysis of BcsC-TPR (Asp24-Leu664) in solution by size exclusion chromatography-small-angle X-ray scattering. The flexibility at the α-helical hinge may play important role for exporting glucan chains.

One-Step Production of Amphiphilic Nanofibrillated Cellulose Using a Cellulose-Producing Bacterium.
Kenji Tajima, Ryo Kusumoto, Ryota Kose, Hiroyuki Kono, Tokuo Matsushima, Takuya Isono, Takuya Yamamoto, Toshifumi Satoh
Biomacromolecules, 18, 10, 3432, 3438, AMER CHEMICAL SOC, 09 Oct. 2017, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, Nanofibrillated bacterial cellulose (NFBC) is produced by culturing a cellulose-producing bacterium (Gluconacetobacter intermedius NEDO-01) with rotation or agitation in medium supplemented with carboxymethylcellulose (CMC). Despite a high yield and dispersibility in water, the product immediately aggregates in organic solvents. To broaden its applicability, we prepared amphiphilic NFBC by culturing strain NEDO-01 in medium supplemented with hydroxyethylcellulose or hydroxypropylcellulose instead of CMC. Transmission electron microscopy analysis revealed that the resultant materials (HE-NFBC and HP-NFBC, respectively) comprised relatively uniform fibers with diameters of 33 ± 7 and 42 ± 8 nm, respectively. HP-NFBC was dispersible in polar organic solvents such as methanol, acetone, isopropyl alcohol, acetonitrile, tetrahydrofuran (THF), and dimethylformamide, and was also dispersible in poly(methyl methacrylate) (PMMA) by solvent mixing using THF. HP-NFBC/PMMA composite films were highly transparent and had a higher tensile strength than neat PMMA film. Thus, HP-NFBC has a broad range of applications, including as a filler material.

Structural and mechanical characterization of bacterial cellulose-polyethylene glycol diacrylate composite gels
Yukari Numata, Hiroyuki Kono, Minato Tsuji, Kenji Tajima
CARBOHYDRATE POLYMERS, 173, 67, 76, ELSEVIER SCI LTD, Oct. 2017, [Peer-reviewed], [Last author]
English, Scientific journal, This study explores the structural and mechanical properties of bacterial cellulose-polyethylene glycol diacrylate (BC-PEGDA) composite gels. The molecular dynamics results obtained by solid-state (13) C nuclear magnetic resonance analyses suggested that BC and PEGDA molecules were incompatible as composite gels, though BC fibers and PEGDA interact with each other. The mechanical strength of the gels depended on the amount of PEGDA, becoming softer and more stretchable when a tensile force was applied, but for a large amount of PEGDA, they became brittle. The BC-3% and 5% PEGDA gels had similar viscoelastic behaviors as a BC gel, and these composite gels could stick to human skin. Since BC-PEGDA composite gels are composed of BC and PEGDA-both of which are biocompatible, it is thought that these composite gels also have excellent biocompatibility. Taken together, we concluded that the BC-3% and 5% PEGDA gels have great potential for use in medical and cosmetic fields. (C) 2017 Elsevier Ltd. All rights reserved.

A facile strategy for manipulating micellar size and morphology through intramolecular cross-linking of amphiphilic block copolymers
Ryoto Tanaka, Kodai Watanabe, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
POLYMER CHEMISTRY, 8, 23, 3647, 3656, ROYAL SOC CHEMISTRY, Jun. 2017, [Peer-reviewed]
English, Scientific journal, The effect of intramolecular cross-linking in an amphiphilic block copolymer (BCP) system was systematically investigated in terms of its thermal properties, critical micelle concentration (CMC), and aqueous self-assembly. A series of linear BCPs consisting of poly(ethylene glycol) (PEG) as a hydrophilic block and poly(epsilon-caprolactone-co-7-allyloxepan-2-one) (P(CL-co-ACL)) as a hydrophobic block were prepared by the ring-opening copolymerization of epsilon-caprolactone (CL) and 7-allyloxepan-2-one (ACL) using poly (ethylene glycol) monomethyl ether as an initiator. The intramolecular olefin metathesis reaction in the P(CL-co-ACL) block was subsequently carried out under various conditions to prepare the cross-linked BCPs with different degrees of cross-linking. The thermal analysis confirmed that the linear P(CL-co-ACL) block was found to crystallize, while the cross-linked one showed no crystallinity. In addition, glass transition temperature of the P(CL-co-ACL) block increased upon cross-linking. On the other hand, the intramolecular cross-linking had no significant influence on the CMC. The self-assembled micelles were prepared from the obtained BCPs and their size and morphology were investigated. For the BCPs with relatively short PEG chains, the micellar size decreased from 36.6 nm to 16.7 nm as the degree of cross-linking of the P(CL-co-ACL) block increased. On the other hand, the BCPs with relatively long PEG chains showed a change in the micellar morphology from spherical micelles to short worm and large compound micelles upon cross-linking.

NMR characterization of methylcellulose: Chemical shift assignment and mole fraction of monomers in the polymer chains
Hiroyuki Kono, Sayaka Fujita, Kenji Tajima
CARBOHYDRATE POLYMERS, 157, 728, 738, ELSEVIER SCI LTD, Feb. 2017, [Peer-reviewed], [Last author]
English, Scientific journal, Methylcellulose samples with different degrees of substitution were prepared by a heterogeneous reaction of cellulose. Two-dimensional NMR spectroscopy provided complete assignment of the H-1 and C-13 chemical shifts of the un-, 2-mono-, 3-mono-, 6-mono-, 2,3-di-, 2,6-di-, 3,6-di-, and 2,3,6-tri-substituted anhydroglucose units (AGUs). Comparative analysis of the chemical shift data revealed the relationship between the methyl substituents at the 2-, 3-, and 6-positions and the 1H and 13C chemical shifts of the AGUs; additivity could be applied to the H-1 and C-13 chemical shifts of methylcellulose. Quantitative C-13 NMR spectra of the samples determined the composition of the eight AGUs and the substituent distribution at the 2-, 3-, and 6-positions of cellulose. This provided estimations of the hydroxyl group reactivity toward methylation and the interactions between the substituent groups within the same AGU. (C) 2016 Elsevier Ltd. All rights reserved.

Synthesis of Well-Defined Three- and Four-Armed Cage-Shaped Polymers via "Topological Conversion" from Trefoil- and Quatrefoil-Shaped Polymers
Yusuke Satoh, Hirohiko Matsuno, Takuya Yamamoto, Kenji Tajima, Takuya Isono, Toshifumi Satoh
MACROMOLECULES, 50, 1, 97, 106, AMER CHEMICAL SOC, Jan. 2017, [Peer-reviewed]
English, Scientific journal, This paper describes a novel synthetic approach for three- and four-armed cage-shaped polymers based on the topological conversion of the corresponding trefoil- and quatrefoil-shaped precursors. The trefoil- and quatrefoil-shaped polymers were synthesized by the following three reaction steps: (1) the t-Bu-P-4-catalyzed ring-opening polymerization of butylene oxide using multiple hydroxy- and azido-functionalized initiators to produce the three- or four-armed star-shaped polymers possessing three or four azido groups at the focal point, respectively, (2) the omega-end modification to install a propargyl group at each chain end, and (3) the intramolecular multiple click cyclization of the clickable star-shaped precursors. The topological conversion from the trefoil- and quatrefoil-shaped polymers to the cage-shaped polymers was achieved by the catalytic hydrogenolysis of the benzyl ether linkages that had been installed at the focal point. The amphiphilic cage-shaped block copolymers together with the corresponding trefoil- and quatrefoil-shaped counterparts were synthesized in a similar way using 2-(2-(2-methoxyethoxy)ethoxy)ethyl glycidyl ether as a hydrophilic monomer and decyl glycidyl ether as a hydrophobic monomer. Interestingly, significant changes in the critical micelle concentration and micellar morphology were observed for the amphiphilic block copolymers upon the topological conversion from the trefoil- and quatrefoil-shaped to cage-shaped architectures.

Heterologous expression of cellulose synthases and trial of synthesis of cellulose in vitro to elucidate the mechanism of cellulose synthesis in nature
Naoki Sunagawa, Kenji Tajima, Masahiro Samejima, Kiyohiko Igarashi
Kobunshi, 66, 6, 288, 289, 2017, [Peer-reviewed]
Japanese, Scientific journal, Cellulose is one of the major carbohydrate on the earth and is utilized in humankind life as a major component of plant materials. Most of terrestrial cellulose is produced by land plant, however it is also found in some algae, tunicates and various bacteria species. Since cellulose biosynthetic process is a complicated system, details are still unknown. To solve this issue, we attempt to make a heterogonous expression system and trying a dynamic visualization of this synthesis process. In this paper, to carry out the approaches, we will report the construction of the recombinant expression system of bacterial cellulose synthase (GrCeSAB) and plant cellulose synthase (AtCeSA1) in the yeast Pichia pastoris.

In vitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria
Kenji Tajima, Xuerong Han, Yoshiki Hashimoto, Yasuharu Satoh, Toshifumi Satoh, Seiichi Taguchi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122, 6, 660, 665, SOC BIOSCIENCE BIOENGINEERING JAPAN, Dec. 2016, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, Thermostable enzymes are required for the rapid and sustainable production of polyhydroxyalkanoate (PHA) in vitro. The in vitro synthesis of PHA using the engineered thermostable synthase PhaC1(SG)(STQK) has been reported; however, the non-thermostable enzymes acetyl-CoA synthetase (ACS) and CoA transferase (CT) from mesophilic strains were used as monomer-supplying enzymes in this system. In the present study, acs and ct were cloned from the thermophilic bacteria Pelotomaculum thermopropionicum JCM10971 and Thermus thermophilus JCM10941 to construct an in vitro PHA synthesis system using only thermostable enzymes. ACS from P. thermopropionicum (ACS(Pt)) and CT from I thermophilus (CTTt) were confirmed to have high thermostability, and their optimal temperatures were around 60 degrees C and 75 degrees C, respectively. The in vitro PHA synthesis was successfully performed by ACS(Pt), CTTt, PhaC1(SG)(STQK), and poly(3-hydroxybutyrate) [P(3HB)] was synthesized at 45 degrees C. Furthermore, the yields of P(3HB) and P(lactate-co-3HB) at 37 degrees C were 1.4-fold higher than those of the in vitro synthesis system with non-thermostable ACS and CT from mesophilic strains. Overall, the thermostable ACS and CT were demonstrated to be useful for the efficient in vitro PHA synthesis at relatively high temperatures. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.

NMR characterization of sodium carboxymethyl cellulose 2: Chemical shift assignment and conformation analysis of substituent groups
Hiroyuki Kono, Kazuhiro Oshima, Hisaho Hashimoto, Yuuichi Shimizu, Kenji Tajima
CARBOHYDRATE POLYMERS, 150, 241, 249, ELSEVIER SCI LTD, Oct. 2016, [Peer-reviewed], [Last author]
English, Scientific journal, The chemical shifts of the substituent groups of sodium carboxymethyl cellulose (CMC) were assigned by examining a series of CMC samples with different degrees of substitution. Comparative analysis of the H-1-C-13 heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) spectra allowed the complete assignment of the substituent groups at the 2-, 3-, and 6 -positions of the seven substituted monomers comprising the CMC chains, namely, 2 -mono-, 3 -mono-, 6-mono, 2,3 -di-, 2,6 -di-, 3,6 -di-, and 2,3,6-tri-substituted anhydroglucose units (AGUs). In addition, the mole fractions of the monomers were determined by lineshape analysis of the carbonyl carbon resonances. The comparison between the chemical shifts of the substituents revealed strong interactions between 2 and 3-substituents in the same AGU, and showed that the steric hindrance by a substituent at the 2- or 3-position suppresses subsequent substitution at the adjacent position. (C) 2016 Elsevier Ltd. All rights reserved.

NMR characterization of sodium carboxymethyl cellulose: Substituent distribution and mole fraction of monomers in the polymer chains
Hiroyuki Kono, Kazuhiro Oshima, Hisaho Hashimoto, Yuuichi Shimizu, Kenji Tajima
CARBOHYDRATE POLYMERS, 146, 1, 9, ELSEVIER SCI LTD, Aug. 2016, [Peer-reviewed], [Last author]
English, Scientific journal, A series of sodium carboxymethyl cellulose (CMC) samples with degrees of substitution (DS) from 0.68 to 2.84 were prepared from cellulose by multistep carboxymethylation. Two-dimensional NMR spectroscopic analyses allowed complete assignment of the H-1 and C-13 chemical shifts of the eight anhydroglucose units (AGUs) found in CMC: 2,3,6-tri-, 2,3-di-, 2,6-di-, 3,6-di-, 2-mono-, 3-mono-, 6-mono-, and unsubstituted AGUs. A comparative analysis of the chemical shift data revealed the substituent effects of the carboxymethyl groups at the 2-, 3-, and 6-positions on the H-1 and C-13 chemical shifts of the AGUs and that additivity could be applied to the H-1 and C-13 chemical shifts of CMC. Quantitative C-13 NMR spectra of the CMC samples allowed determination of the AGU composition, as well as the substituent distribution at the 2-, 3-, and 6-positions of cellulose, which allowed estimation of the reactivity of the hydroxyl groups toward the carboxymethylation. (C) 2016 Elsevier Ltd. All rights reserved.

Self-Assembly of Maltoheptaose-block-polycaprolactone Copolymers: Carbohydrate-Decorated Nanoparticles with Tunable Morphology and Size in Aqueous Media
Takuya Isono, Kana Miyachi, Yusuke Satoh, Ryosuke Nakamura, Yao Zhang, Issei Otsuka, Kenji Tajima, Toyoji Kakuchi, Redouane Borsali, Toshifumi Satoh
MACROMOLECULES, 49, 11, 4178, 4194, AMER CHEMICAL SOC, Jun. 2016, [Peer-reviewed]
English, Scientific journal, This paper describes the systematic investigation into the aqueous self-assembly of a series of block copolymers (BCPs) consisting of maltoheptaose (MH; as the A block) and poly(e-caprolactone) (PCL; as the B block), i.e., linear AB-type diblock copolymers with varied PCL molecular weights (MH-b-PC1-(2.5(k,3.3k,5k10k))), AB(y)-type = 2, MH-b(PCL5k) 2; y = 3, MH-b-(PCL3.3k)(3)), A(2)B(2)-type ((MH)(2)-b(PCL(5)k)(2)), and AxB-type miktoarm star polymers (x = 2, (MH)2-b-PCL10k; x = 3, (MH)(3)-b-PCLiok), which had been precisely synthesized via the combination of the living ring opening polymerization and click reaction. Under similar conditions, the nanoprecipitation method was employed to self -assemble them in an aqueous medium. Imaging and dynamic light scattering techniques indicated the successful formation of the carbohydrate -decorated nanoparticles via self -assembly. The MH-b-PCLs formed regular core shell micellar nanoparticles with the hydrodynamic "radius (Rh) of 17-43 nm. MH-b-(PCL5k)2 and MH-b-(PCL3.3k)(3), which have an NPCL comparable to MH-b-PCLiok, were found to form large compound micelles with relatively large radii (Rh of 49 and 56 nm, respectively). On the other hand, (MH),-b-(PCL5k),, (MH),-b-PCLiok, and (MH)3-bPCLiok predominantly formed the regular core shell micellar nanoparticles (Rh = 29-39 nm) with a size smaller than that of MH-b-PCLiok (Rh = 43 nm).

Advanced functionalization of polyhydroxyalkanoate via the UV-initiated thiol-ene click reaction
Kenji Tajima, Kosuke Iwamoto, Yasuharu Satoh, Ryosuke Sakai, Toshifumi Satoh, Tohru Dairi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100, 10, 4375, 4383, SPRINGER, May 2016, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, Polyhydroxyalkanoates (PHAs) incorporating vinyl-bearing 3-hydroxyalkanoates were prepared in 8.5-12.9 g L-1 yield. The molar ratios (0-16 mol%) of the vinyl-bearing 3-hydroxyalkanoate derivatives were controlled by the continuous feeding of undecylenate at various concentrations. Subsequently, the PHAs were functionalized by UV-initiated thiol-ene click reaction and chemical modification. H-1 NMR spectra suggested that 3-mercaptopropionic acid and 2-aminoethanethiol were successfully introduced into the vinyl-bearing PHA. Subsequently, chemical modification using fluorescein or a fibronectin active fragment (GRGDS) was attempted. The former yielded a PHA derivative capable of emitting fluorescence under UV irradiation, which was useful for determining the miscibility of PHA in a composite film comprising poly-EY-lactic acid (PLLA) and PHA. In the latter case, PHA bearing GRGDS peptides exhibited cell adhesiveness, suggesting that its biocompatibility was improved upon peptide introduction. Taken together, the UV-initiated thiol-ene click reaction was demonstrated to be useful in PHA modification.

Mechanical properties of a bacterial cellulose/polyethylene glycol gel with a peripheral region crosslinked by polyethylene glycol diacrylate
Yukari Numata, Shiro Masaki, Kenji Tajima
POLYMER JOURNAL, 48, 3, 317, 321, NATURE PUBLISHING GROUP, Mar. 2016, [Peer-reviewed], [Last author]
English, Scientific journal

Synthesis of Well-Defined Amphiphilic Star-Block and Miktoarm Star Copolyethers via t-Bu-P-4-Catalyzed Ring-Opening Polymerization of Glycidyl Ethers
Yusuke Satoh, Kana Miyachi, Hirohiko Matsuno, Takuya Isono, Kenji Tajima, Toyoji Kakuchi, Toshifumi Satoh
MACROMOLECULES, 49, 2, 499, 509, AMER CHEMICAL SOC, Jan. 2016, [Peer-reviewed]
English, Scientific journal, A comprehensive set of amphiphilic star-shaped block copolyethers with a fixed molecular weight and composition was synthesized via the t-Bu-P-4-catalyzed ring opening polymerization (ROP) of 2-(2-(2-methoxyethoxy)-ethoxy)ethyl glycidyl ether as a hydrophilic monomer and decyl glycidyl ether as a hydrophobic monomer. The three and four-armed star-block copolyethers, i.e., the (AB)(3)-, (BA)(3)-, (AB)(4)-, and (BA)(4)-type star-block copolyethers, where A and B represent hydrophilic and hydrophobic blocks, respectively, were synthesized by the sequential t-Bu-P-4-catalyzed block copolymerization using tri- and tetra-alcohol initiators, respectively, according to the core-first method. The homogeneous growth of each arm was confirmed by cleaving the linkages between the initiator residue and polyether arms. The synthesis of the A(2)B(2)-, AB(2)-, and A(2)B-type miktoarm star copolyethers was achieved by the combination of the t-Bu-P-4-catalyzed ROP and azido-alkyne click chemistry. The azido- and ethynyl-functionalized precursor polyethers with the predicted molecular weights were separately prepared by the t-Bu-P-4-catalyzed ROP with the aid of functional initiators as well as a terminator. The intermolecular click reaction of the precursors provided the desired miktoarm star copolyethers. All the obtained star-shaped block copolyethers had a comparable monomer composition (degree of polymerization = 50:50) and total molecular weight (ca. 22 200 g mol(-1)) with a narrow dispersity (<1.05). The hydrodynamic diameter and the cloud point analyses for the aqueous micellar solution of the amphiphilic star-shaped block copolyethers revealed that the self-assembly properties were affected by the block arrangements and branched architectures of the amphiphilic polymers.

Intramolecular olefin metathesis as a robust tool to synthesize single-chain nanoparticles in a size-controlled manner
Kodai Watanabe, Ryoto Tanaka, Kenji Takada, Myung-Jin Kim, Jae-Suk Lee, Kenji Tajima, Takuya Isono, Toshifumi Satoh
POLYMER CHEMISTRY, 7, 29, 4782, 4792, ROYAL SOC CHEMISTRY, 2016, [Peer-reviewed]
English, Scientific journal, A comprehensive examination of the synthesis of single-chain nanoparticles (SCNPs) from statistical copolymers of n-butyl methacrylate (BMA) and 3-butenyl methacrylate (3BMA), i.e., P(BMA-co-3BMA)s, via the intramolecular olefin metathesis reaction under high dilution conditions is described. The olefin metathesis reaction of P(BMA-co-3BMA) using Grubbs' 2nd generation catalyst in CH2Cl2 efficiently gave the corresponding SCNPs under mild conditions. We achieved the size-controlled synthesis of the SCNPs by adjusting the following factors: (1) the olefin content in the precursor, (2) the molecular weight of the precursor, and (3) the solvent quality of the reaction medium. The hydrodynamic radius and the intrinsic viscosity of the resultant SCNPs were investigated by DLS and viscometric measurements, respectively, which provided further evidence of SCNP formation with controlled diameters. Furthermore, the above-established intramolecular olefin metathesis approach was successfully applied to poly(acrylate), poly(styrene), and poly(ester) precursors, which proved that the present approach could be applied to a wide range of olefin-containing precursors to give SCNPs with various functional groups.

In Vivo Curdlan/Cellulose Bionanocomposite Synthesis by Genetically Modified Gluconacetobacter xylinus
Ju Fang, Shin Kawano, Kenji Tajima, Tetsuo Kondo
BIOMACROMOLECULES, 16, 10, 3154, 3160, AMER CHEMICAL SOC, Oct. 2015, [Peer-reviewed]
English, Scientific journal, Bacterial cellulose pellicle produced by Gluconacetobacter xylinus (G. xylinus) is one of the best biobased materials having a unique supernetwork structure with remarkable physiochemical properties for a wide range of medical and tissue-engineering applications. It is still necessary to modify them to obtain materials suitable for biomedical use with satisfactory mechanical strength, biodegradability, and bioactivity. The aim of this research was to develop a gene-transformation route for the production of bacterial cellulose/Curdlan (beta-1,3-glucan) nanocomposites by separate but simultaneous in vivo synthesis of cellulose and Curdlan. Modification of the cellulose-nanofiber-producing system of G. xylinus enabled Curdlan to be synthesized simultaneously with cellulose nanofibers in vivo, resulting in biopreparation of nanocomposites. The obtained Curdlan/cellulose composites were characterized, and their properties were compared with those of normal bacterial cellulose pellicles, indicating that Curdlan mixed with the cellulose nanofibers at the nanoscale without disruption of the nanofiber network structure in the pellicle.

Controlled/Living Ring-Opening Polymerization of Glycidylamine Derivatives Using t-Bu-P-4/Alcohol Initiating System Leading to Polyethers with Pendant Primary, Secondary, and Tertiary Amino Groups
Takuya Isono, Shunsuke Asai, Yusuke Satoh, Toshimitsu Takaoka, Kenji Tajima, Toyoji Kakuchi, Toshifumi Satoh
MACROMOLECULES, 48, 10, 3217, 3229, AMER CHEMICAL SOC, May 2015, [Peer-reviewed]
English, Scientific journal, The combination of t-Bu-P-4 and alcohol was found to be an excellent catalytic system for the controlled/living ring-opening polymerization (ROP) of N,N-disubstituted glycidylamine derivatives, such as N,N-dibenzylglycidylamine (DBGA), N-benzyl-N-methylglycidylamine, N-glycidylmorpholine, and N,N-bis(2-methoxyethyl)glycidylamine, to give well-defined polyethers having various pendant tertiary amino groups with predictable molecular weights and narrow molecular weight distributions (typically M-w/M-n, < 1.2). The tBu-P-4-catalyzed ROP of these monomers in toluene at room temperature proceeded in a living manner, which was confirmed by a MALDI-TOF MS analysis, kinetic measurement, and postpolymerization experiment. The well-controlled nature of the present system enabled the production of the block copolymers composed of the glycidylamine monomers. The polyethers having pendant primary and secondary amino groups, i.e., poly(glycidylamine) and poly(glycidylmethylamine), respectively, were readily obtained by the debenzylation of poly(DBGA) and poly(BMGA), respectively, through the treatment with Pd/C in THF/MeOH under a hydrogen atmosphere. To the best of our knowledge, this report is the first example of the controlled/living polymerization of glycidylamine derivatives, providing a rapid and comprehensive access to the polyethers having primary, secondary, and tertiary amino groups.

Bacterial cellulose gels with high mechanical strength
Yukari Numata, Tadanori Sakata, Hidemitsu Furukawa, Kenji Tajima
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 47, 57, 62, ELSEVIER SCIENCE BV, Feb. 2015, [Peer-reviewed], [Last author]
English, Scientific journal, A composite structure was formed between polyethylene glycol diacrylate (PEGDA) and bacterial cellulose (BC) gels swollen in polyethylene glycol (PEG) as a solvent (BC/PEG gel) to improve the mechanical strength of the gels. The mechanical strength under compression and the rheostatic properties of the gels were evaluated. The compression test results indicated that the mechanical strength of the gels depended on the weight percent of cross-linked PEGDA in the gel, the chain length between the cross-linking points, and the cross-linking density of PEGDA polymers. The PEGDA polymers around the cellulose fibers were resistant to pressure; thus, the BC/PEG-PEGDA gel was stronger than the BC/PEG gel under compression. The results of transmittance measurements and thermomechanical analysis showed that the rheostatic properties of the gels were retained even after composite structure formation. BC/PEG-PEGDA gels, which are expected to be biocompatible, may be useful for clinical applications as a soft material. (C) 2014 Elsevier B.V. All rights reserved.

Organophosphate-catalyzed bulk ring-opening polymerization as an environmentally benign route leading to block copolyesters, end-functionalized polyesters, and polyester-based polyurethane
Tatsuya Saito, Yusuke Aizawa, Kenji Tajima, Takuya Isono, Toshifumi Satoh
POLYMER CHEMISTRY, 6, 24, 4374, 4384, ROYAL SOC CHEMISTRY, 2015, [Peer-reviewed]
English, Scientific journal, The ring-opening polymerizations (ROPs) of epsilon-caprolactone (epsilon-CL), delta-valerolactone, 1,5-dioxepan-2-one, trimethylene carbonate, and L-lactide were performed in the bulk using an organophosphate, such as diphenyl phosphate, bis(4-nitrophenyl)phosphate, and di(2,6-xylyl)phosphate, as the catalyst. The ROPs proceeded in a well-controlled manner even under the bulk conditions to afford well-defined aliphatic polyesters, polyester-ether, and polycarbonate with relatively low dispersities. Notably, the amount of the loaded catalyst was successfully reduced when compared to the conventional organophosphate-catalyzed ROP in solution. A kinetic study revealed the controlled/living nature of the present bulk ROP system, which allowed us to produce the block copolymers composed of polyesters, polyester-ether, and polycarbonate in one pot. Syntheses of the end-functionalized poly(epsilon-caprolactone)s (PCLs) and poly(trimethylene carbonate) were successfully demonstrated using alcohol initiators possessing highly reactive functional groups. Furthermore, the alpha,beta-dihydroxy telechelic PCL-diol as well as the three-and fourarmed star-shaped PCL-polyols were also easily obtained by using the polyols as an initiator. Finally, the one-pot synthesis of polyurethane via the ROP of epsilon-CL and a subsequent urethane forming reaction was demonstrated by taking advantage of the dual catalytic abilities of the organophosphate for both the ROP and polyurethane synthesis.

Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene
Xuerong Han, Yasuharu Satoh, Yumi Kuriki, Teruyuki Seino, Shinji Fujita, Takanori Suda, Takanori Kobayashi, Kenji Tajima
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 118, 5, 514, 519, SOC BIOSCIENCE BIOENGINEERING JAPAN, Nov. 2014, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, We successfully isolated one microorganism (UMI-21) from Ulva, a green algae that contains starch. The strain UMI-21 can produce polyhydroxyalkanoate (PHA) from starch, maltotriose, or maltose as a sole carbon source. Taxonomic studies and 16S rDNA sequence analysis revealed that strain UMI-21 was phylogenetically related to species of the genus Massilia. The PHA content under the cultivation condition using a 10-L jar fermentor was 45.5% (w/w). This value was higher than that obtained after cultivation in a flask, suggesting the possibility of large-scale PHA production by UMI-21 from starch. A major issue for the industrial production of microbial PHAs is the very high production cost. Starch is a relatively inexpensive substrate that is also found in abundant seaweeds such as Ulva. Therefore, the strain isolated in this study may be very useful for producing PHA from seaweeds containing polysaccharides such as starch. In addition, a 3.7-kbp DNA fragment containing the whole PHA synthase gene (phaC) was obtained from the strain UMI-21. The results of open reading frame (ORF) analysis suggested that the DNA fragment contained two ORFs, which were composed of 1740 (phaC) and 564 bp (phaR). The deduced amino acid sequence of PhaC from strain UMI-21 shared high similarity with PhaC from Ralstonia eutropha, which is a representative PHA-producing bacterium with a class I PHA synthase. This is the first report for the cloning of the PHA synthase gene from Massilia species. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

Stereoblock-like Brush Copolymers Consisting of Poly(L-lactide) and Poly(D-lactide) Side Chains along Poly(norbornene) Backbone: Synthesis, Stereocomplex Formation, and Structure-Property Relationship
Takuya Isono, Yohei Kondo, Shun Ozawa, Yougen Chen, Ryosuke Sakai, Shin-ichiro Sato, Kenji Tajima, Toyoji Kakuchi, Toshifumi Satoh
MACROMOLECULES, 47, 20, 7118, 7128, AMER CHEMICAL SOC, Oct. 2014, [Peer-reviewed]
English, Scientific journal, Random and block copolymerizations of poly(l-lactide) (PLLA) and poly(d-lactide) (PDLA) macromonomers having an exo-norbornene group at the alpha- or pi-chain end (D/L ratio = 1/1, M-n = ca. 5000 g mol(-1)) were performed via ring-opening metathesis polymerization to produce the brush random and block copolymers consisting of parallel or antiparallel aligned PLLA and PDLA side chains on a poly(norbornene) backbone. The molecular weight and polydispersity index of the brush copolymers were in the range of 40 300-458 000 g mol(-1) and 1.031.14, respectively. Despite such high molecular weights, these brush copolymers formed a stereocomplex without homochiral crystallization. The melting temperature (T-m) and crystallinity (X) of the resulting stereocomplex varied depending on the backbone length, relative chain direction, and distribution of the PLLA/PDLA side chains. The parallel brush copolymers showed significantly higher T-m and X values than the antiparallel ones.

Characterization of an Alginate Lyase, FlAlyA, from Flavobacterium sp Strain UMI-01 and Its Expression in Escherichia coli
Akira Inoue, Kohei Takadono, Ryuji Nishiyama, Kenji Tajima, Takanori Kobayashi, Takao Ojima
MARINE DRUGS, 12, 8, 4693, 4712, MDPI AG, Aug. 2014, [Peer-reviewed]
English, Scientific journal, A major alginate lyase, FlAlyA, was purified from the periplasmic fraction of an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. FlAlyA showed a single band of similar to 30 kDa on SDS-PAGE and exhibited the optimal temperature and pH at 55 degrees C and pH 7.7, respectively. Analyses for substrate preference and reaction products indicated that FlAlyA was an endolytic poly(mannuronate) lyase (EC 4.2.2.3). A gene fragment encoding the amino-acid sequence of 288 residues for FlAlyA was amplified by inverse PCR. The N-terminal region of 21 residues except for the initiation Met in the deduced sequence was predicted as the signal peptide and the following region of six residues was regarded as propeptide, while the C-terminal region of 260 residues was regarded as the polysaccharide-lyase-family-7-type catalytic domain. The entire coding region for FlAlyA was subjected to the pCold I-Escherichia coli BL21(DE3) expression system and similar to eight times higher yield of recombinant FlAlyA (recFlAlyA) than that of native FlAlyA was achieved. The recFlAlyA recovered in the periplasmic fraction of E. coli had lost the signal peptide region along with the N-terminal 3 residues of propeptide region. This suggested that the signal peptide of FlAlyA could function in part in E. coli.

Synthesis of Linear, Cyclic, Figure-Eight-Shaped, and Tadpole-Shaped Amphiphilic Block Copolyethers via t-Bu-P-4-Catalyzed Ring-Opening Polymerization of Hydrophilic and Hydrophobic Glycidyl Ethers
Takuya Isono, Yusuke Satoh, Kana Miyachi, Yougen Chen, Shin-ichiro Sato, Kenji Tajima, Toshifumi Satoh, Toyoji Kakuchi
MACROMOLECULES, 47, 9, 2853, 2863, AMER CHEMICAL SOC, May 2014, [Peer-reviewed]
English, Scientific journal, This paper describes the synthesis of systematic sets of figure-eight- and tadpole-shaped amphiphilic block copolyethers (BCPs) consisting of poly(decyl glycidyl ether) and poly[2-(2-(2-methoxyethoxy)ethoxy)ethyl glycidyl ether], together with the corresponding cyclic counterparts, via combination of the t-Bu-P-4-catalyzed ring-opening polymerization (ROP) and click cyclization. The clickable linear BCP precursors, with precisely controlled azido and ethynyl group placements as well as a fixed molecular weight and monomer composition (degree of polymerization for each block was adjusted to be around 50), were prepared by the t-Bu-P-4-catalyzed ROP with the aid of functional initiators and terminators. The click cyclization of the precursors under highly diluted conditions produced a series of cyclic, figure-eight-, and tadpole-shaped BCPs with narrow molecular weight distributions of less than 1.06. Preliminary studies of the BCPs self-assembly in water revealed the significant variation in their cloud points depending on the BCP architecture, though there were small architectural effects on their critical micelle concentration and morphology of the aggregates.

One-step production of nanofibrillated bacterial cellulose (NFBC) from waste glycerol using Gluconacetobacter intermedius NEDO-01
Ryota Kose, Naoki Sunagawa, Makoto Yoshida, Kenji Tajima
CELLULOSE, 20, 6, 2971, 2979, SPRINGER, Dec. 2013, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, A major by-product of biodiesel production is waste glycerol, which has numerous potential applications. In this study, we isolated a novel bacterium capable of producing cellulose from waste glycerol, and identified it as a novel strain (named NEDO-01) of Gluconacetobacter intermedius. Scanning electron microscopy revealed that the morphology of the pellicle produced by NEDO-01 was similar to that of cellulose produced by Gluconacetobacter hansenii ATCC23769. Furthermore, X-ray diffraction and solid-state nuclear magnetic resonance spectroscopic analyses suggested that cellulose produced by NEDO-01 had molecular and crystalline structures similar to those of cellulose produced by ATCC23769. After the optimization of cultivation conditions, NEDO-01 mediated the one-step production of nanofibrillated bacterial cellulose (NFBC) from waste glycerol in a medium supplemented with carboxymethyl cellulose. Transmission electron microscopic analysis revealed that the NFBC was composed of relatively uniform fibers with diameters of approximately 20 nm. NFBC was produced as uniform water suspensions, the yield of which was 3.4 g/L from cultivation in 7.5 L medium in a 10-L jar fermenter. The bioconversion of waste glycerol to NFBC, which has superior fluidity, moldability, and miscibility, has a wide variety of applications, including potential uses in the medical and materials engineering fields.

Cellulose complementing factor (Ccp) is a new member of the cellulose synthase complex (terminal complex) in Acetobacter xylinum
Naoki Sunagawa, Takaaki Fujiwara, Takanori Yoda, Shin Kawano, Yasuharu Satoh, Min Yao, Kenji Tajima, Tohru Dairi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115, 6, 607, 612, SOC BIOSCIENCE BIOENGINEERING JAPAN, Jun. 2013, [Peer-reviewed], [Corresponding author]
English, Scientific journal, The cellulose complementing factor (Ccp) is known to be involved in cellulose production in the Acetobacter species. However, its precise functions remain unclear. In the current study, we identified the coding region of the ccpAx gene (ccp gene from Acetobacter xylinum) and the localization of the CcpAx in cells by generating fusion proteins tagged to an enhanced green fluorescent protein (EGFP). From the results of N-terminal sequencing of CcpAx-EGFP-fusion protein, which recovered 65% of cellulose-producing abilities of the wild-type to the ccpAx gene-knockout mutant, the ccpAx gene was determined to encode a protein with the molecular weight of 8 kDa. The amino acid sequence deduced had high similarities with the C-terminal regions of Ccp proteins from other Acetobacter species. Fluorescence microscopy analysis showed that CcpAx was longitudinally localized along with one side of the cell membrane. Additionally, the localization of AxCeSD, which is thought to be a member of the cellulose synthase complex [terminal complex (TC)] in A. xylinum, was determined in the same manner as CcpAx. Fluorescence microscopy analysis showed that AxCeSD had a localization pattern similar to that of CcpAx. Pulldown assays and isothermal titration calorimetry analysis clearly showed a significant interaction between CcpAx and AxCeSD. Taken together, these data strongly suggest that CcpAx functions as a member of the TC in A. xylinum. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

The c-di-GMP recognition mechanism of the PilZ domain of bacterial cellulose synthase subunit A
Takaaki Fujiwara, Keisuke Komoda, Naofumi Sakurai, Kenji Tajima, Isao Tanaka, Min Yao
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 431, 4, 802, 807, ACADEMIC PRESS INC ELSEVIER SCIENCE, Feb. 2013, [Peer-reviewed]
English, Scientific journal, In some Proteobacteria and Firmicutes such as Pseudomonas aeruginosa, Vibrio cholerae, Xanthomonas campestris, and Clostridium difficile, cyclic dimeric guanosine monophosphate (c-di-GMP) is known to regulate cellular processes, including motility, biofilm formation, and virulence, as a second messenger. Cellulose production in Acetobacter xylinum, a model organism of cellulose biosynthesis, also depends on by cellular c-di-GMP level. In cellulose-synthesizing bacteria, a terminal complex localized in the cell membrane synthesizes cellulose and regulates the production of cellulose sensed by c-di-GMP. Although previous studies indicated that the PilZ domain conserved in cellulose synthase subunit A (CeSA) was part of a receptor for c-di-GMP, the recognition mechanism by PilZ domain of CeSA remains unclear. In the present study, we studied the interaction between c-di-GMP and the PilZ domain of CeSA from a structural viewpoint. First, we solved the crystal structure of the PilZ domain of CeSA from A. xylinum (AxCeSA-PilZ) at 2.1 angstrom resolution. Then, comparison of the sequence and structure of AxCeSA-PilZ to those of known structures of PilZ, such as VCA0042, PP4397, and PA4608, indicated the involvement of Lys573 and Arg643 of AxCeSA-PilZ in the recognition of c-di-GMP besides the RxxxR motif. Finally, the binding characteristics of c-di-GMP to AxCeSA-PilZ and mutants were determined with isothermal titration calorimetry, indicating that the residues corresponding to Lys573 and Arg643 in AxCeSA-PilZ generally contribute to the binding of c-di-GMP to PilZ. (C) 2013 Elsevier Inc. All rights reserved.

Cellulose production by Enterobacter sp CJF-002 and identification of genes for cellulose biosynthesis
Naoki Sunagawa, Kenji Tajima, Mariko Hosoda, Shin Kawano, Ryota Kose, Yasuharu Satoh, Min Yao, Tohru Dairi
CELLULOSE, 19, 6, 1989, 2001, SPRINGER, Dec. 2012, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Enterobacter sp. CJF-002, which had been isolated as a cellulose producer with saccharides as a carbon source, was shown to efficiently produce cellulose from beet molasses (B-Mol) and biodiesel fuel by-product (BDF-B), renewable non-edible and inexpensive biomasses. The cellulose production rates of Enterobacter sp. CJF-002 using B-Mol and BDF-B as carbon sources were faster than those of Acetobacter xylinum (A. xylinum) ATCC23769, a representative cellulose producing bacterium. To clarify the biosynthetic machinery of cellulose in the strain, genes responsible for cellulose biosynthesis were cloned. Six open reading frames (ORFs) were suggested to be clustered and their amino acid sequences had high similarities with those of BcsA, BcsB, BcsZ (endoglucanase), BcsC, YhjQ, and YhjK from Escherichia coli, respectively. Of these, the former four genes showed low similarities to corresponding orthologs in a cellulose biosynthetic gene cluster of A. xylinum. A bcsC-knockout mutant produced no cellulose, confirming that the gene is essential for cellulose production of Enterobacter sp. CJF-002. The predicted three-dimensional structure of BcsZ(En) from Enterobacter sp. CJF-002 had high similarity with that of CMCax (endoglucanase) from A. xylinum ATCC23769 in spite of the low similarity in their amino acid sequences. Taken together, A. xylinum and Enterobacter sp. CJF-002 might produce cellulose via a similar synthetic mechanism.

Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol
Yasuharu Satoh, Kenji Tajima, Masanobu Munekata, Jay D. Keasling, Taek Soon Lee
METABOLIC ENGINEERING, 14, 6, 603, 610, ACADEMIC PRESS INC ELSEVIER SCIENCE, Nov. 2012, [Peer-reviewed]
English, Scientific journal, The hydroxylation of tyrosine is an important reaction in the biosynthesis of many natural products. The use of bacteria for this reaction has not been very successful due to either the over-oxidation to ortho-quinone when using tyrosinases from bacteria or plants, or the lack of the native cofactor, tetrahydrobiopterin (BH4), needed for the activity of tyrosine hydroxylases (TH). Here, we demonstrate that an Escherichia coli cofactor, tetrahydromonapterin (MH4), can be used as an alternative cofactor for TH in presence of the BH4 regeneration pathway, and tyrosine hydroxylation is performed without over-oxidation. We used this platform for biosynthesis of one of the most powerful antioxidants, hydroxytyrosol. An endogenous aromatic aldehyde oxidase was identified and knocked out to prevent formation of the side product, and this resulted in nearly exclusive production of hydroxytyrosol in engineered E. coli. Finally, hydroxytyrosol production from a simple sugar as a sole carbon source was demonstrated. Published by Elsevier Inc.

In vitro synthesis of polyhydroxyalkanoate (PHA) incorporating lactate (LA) with a block sequence by using a newly engineered thermostable PHA synthase from Pseudomonas sp SG4502 with acquired LA-polymerizing activity
Kenji Tajima, Xuerong Han, Yasuharu Satoh, Ayako Ishii, Yuji Araki, Masanobu Munekata, Seiichi Taguchi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94, 2, 365, 376, SPRINGER, Apr. 2012, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, Recently, we succeeded in isolating a thermotolerant bacterium, Pseudomonas sp. SG4502, which is capable of accumulating polyhydroxyalkanoate (PHA) even at 55 A degrees C, as a source of thermostable enzymes. In this study, we cloned a pha locus from the bacterium and identified two genes encoding PHA synthases (PhaC1(SG) and PhaC2(SG)). Two mutations, Ser324Thr and Gln480Lys, corresponding to those of a lactate (LA)-polymerizing enzyme (LPE) from mesophilic Pseudomonas sp. 61-3 were introduced into PhaC1(SG) to evaluate the potential of the resulting protein as a "thermostable LPE". The mutated PhaC1(SG) [PhaC1(SG)(STQK)] showed high thermal stability in synthesizing P(LA-co-3HB) in an in vitro reaction system under a range of high temperatures. Requirement of 3HBCoA as a priming unit for LA polymerization by the LPE has been suggested in both of the in vitro and in vivo experiments. Based on the finding, the PhaC1(SG)(STQK)-mediated synthesis of a LA-based copolymer with a block sequence was achieved in the in vitro system by sequential feeding of the corresponding two substrates. This in vitro reaction system using the thermostable LPE provides us with a versatile way to synthesize the various types of LA-based copolymers with desired sequence patterns, random or block, depending on the way of supplying hydroxyalkanoates (mixed or sequential feeding).

Engineering of a Tyrosol-Producing Pathway, Utilizing Simple Sugar and the Central Metabolic Tyrosine, in Escherichia coli
Yasuharu Satoh, Kenji Tajima, Masanobu Munekata, Jay D. Keasling, Taek Soon Lee
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60, 4, 979, 984, AMER CHEMICAL SOC, Feb. 2012, [Peer-reviewed]
English, Scientific journal, Metabolic engineering was applied to the development of Escherichia coli capable of synthesizing tyrosol hydroxyphenyl)ethanol), an attractive phenolic compound with great industrial value, from glucose, a renewable carbon source. In this strain, tyrosine, which was supplied not only from the culture medium but also from the central metabolism, was converted into tyrosol via three steps: decarboxylation, amine oxidation, and reduction. The engineered strain synthesized both tyrosol and 4-hydroxyphenylacetate (4HPA), but disruption of the endogenous phenylacetaldehyde dehydrogenase gene shut off 4HPA production and improved the production of tyrosol as a sole product. The engineered mutant strain was capable of producing 0.5 mM tyrosol from 1% (w/v) glucose during a 48 h shake flask cultivation.

Chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA) incorporating 2-hydroxybutyrate by wild-type class I PHA synthase from Ralstonia eutropha
Xuerong Han, Yasuharu Satoh, Toshifumi Satoh, Ken&apos, ichiro Matsumoto, Toyoji Kakuchi, Seiichi Taguchi, Tohru Dairi, Masanobu Munekata, Kenji Tajima
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92, 3, 509, 517, SPRINGER, Nov. 2011, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal, A previously established improved two-phase reaction system has been applied to analyze the substrate specificities and polymerization activities of polyhydroxyalkanoate (PHA) synthases. We first analyzed the substrate specificity of propionate coenzyme A (CoA) transferase and found that 2-hydroxybutyrate (2HB) was converted into its CoA derivative. Then, the synthesis of PHA incorporating 2HB was achieved by a wild-type class I PHA synthase from Ralstonia eutropha. The PHA synthase stereoselectively polymerized (R)-2HB, and the maximal molar ratio of 2HB in the polymer was 9 mol%. The yields and the molecular weights of the products were decreased with the increase of the (R)-2HB concentration in the reaction mixture. The weight-average molecular weight of the polymer incorporating 9 mol% 2HB was 1.00 x 10(5), and a unimodal peak with polydispersity of 3.1 was observed in the GPC chart. Thermal properties of the polymer incorporating 9 mol% 2HB were analyzed by DSC and TG-DTA. T (g), T (m), and T (d) (10%) were observed at -1.1A degrees C, 158.8A degrees C, and 252.7A degrees C, respectively. In general, major components of PHAs are 3-hydroxyalkanoates, and only engineered class II PHA synthases have been reported as enzymes having the ability to polymerize HA with the hydroxyl group at C2 position. Thus, this is the first report to demonstrate that wild-type class I PHA synthase was able to polymerize 2HB.

Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate
Y. Satoh, K. Tajima, S. Nakamoto, H. Xuerong, T. Matsushima, T. Ohshima, S. Kawano, T. Erata, T. Dairi, M. Munekata
JOURNAL OF APPLIED MICROBIOLOGY, 111, 4, 811, 817, WILEY-BLACKWELL, Oct. 2011, [Peer-reviewed]
English, Scientific journal, Aims: The aim of this study was to isolate a thermotolerant micro-organism that produces polyhydroxyalkanoates (PHAs) composed of medium-chain-length (mcl) HA units from a biodiesel fuel (BDF) by-product as a carbon source.
Methods and Results: We successfully isolated a thermotolerant micro-organism, strain SG4502, capable to accumulate mcl-PHA from a BDF by-product as a carbon source at a cultivation temperature of 45 degrees C. The strain could also produce mcl-PHA from acetate, octanoate and dodecanoate as sole carbon sources at cultivation temperatures up to 55 degrees C. Taxonomic studies and 16S rRNA gene sequence analysis revealed that strain SG4502 was phylogenetically affiliated with species of the genus Pseudomonas. This study is the first report of PHA synthesis by a thermotolerant Pseudomonas.
Conclusions: A novel thermotolerant bacterium capable to accumulate mcl-PHA from a BDF by-product was successfully isolated.
Significance and Impact of the Study: A major issue regarding industrial production of microbial PHAs is their much higher production cost compared with conventional petrochemical-based plastic materials. Especially significant are the cost of a fermentative substrate and the running cost to maintain a temperature suitable for microbial growth. Thus, strain SG4502, isolated in this study, which assimilates BDF by-product and produces PHA at high temperature, would be very useful for practical application in industry.

Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli
Jumiarti Agus, Prihardi Kahar, Manami Hyakutake, Satoshi Tomizawa, Hideki Abe, Takeharu Tsuge, Yasuharu Satoh, Kenji Tajima
POLYMER DEGRADATION AND STABILITY, 95, 12, 2250, 2254, ELSEVIER SCI LTD, Dec. 2010, [Peer-reviewed]
English, Scientific journal, This study aimed to investigate the factors affecting molecular weight of poly[ (R)-3-hydroxybutyrate] [P(3HB)] when polyhydroxyalkanoate (PHA) synthase (PhaRC(Bsp)) from Bacillus sp. INT005 was used for P(3HB) synthesis in Escherichia coli JM109. It was found that the molecular weight of P(3HB) decreased with time in mid- and late-phase of culture and was strongly affected by culture temperature. At 37 degrees C culture temperature, the molecular weight of P(3HB) rapidly decreased from 4.4 x 10(5) to 4.8 x 10(4) with culture time, whereas it was almost unchanged at 25 degrees C. Kinetic analysis suggested that the decrease in molecular weight of P(3HB) was due to random scission of the polymer chain. The decrease in molecular weight of P(3HB) was not observed when PHA synthases other than PhaRC(Bsp) were expressed. This study sheds light on the unique behaviour in molecular weight change of P(3HB) that is synthesized by E. coli expressing PhaRCB(sp). (C) 2010 Elsevier Ltd. All rights reserved.

Structure of bacterial cellulose synthase subunit D octamer with four inner passageways
Song-Qing Hu, Yong-Gui Gao, Kenji Tajima, Naoki Sunagawa, Yong Zhou, Shin Kawano, Takaaki Fujiwara, Takanori Yoda, Daisuke Shimura, Yasuharu Satoh, Masanobu Munekata, Isao Tanaka, Min Yao
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107, 42, 17957, 17961, NATL ACAD SCIENCES, Oct. 2010, [Peer-reviewed], [Lead author]
English, Scientific journal, The cellulose synthesizing terminal complex consisting of subunits A, B, C, and D in Acetobacter xylinum spans the outer and inner cell membranes to synthesize and extrude glucan chains, which are assembled into subelementary fibrils and further into a ribbon. We determined the structures of subunit D (AxCeSD/AxBcsD) with both N- and C-terminal His(6) tags, and in complex with cellopentaose. The structure of AxCeSD shows an exquisite cylinder shape (height: similar to 65 angstrom, outer diameter: similar to 90 angstrom, and inner diameter: similar to 25 angstrom) with a right-hand twisted dimer interface on the cylinder wall, formed by octamer as a functional unit. All N termini of the octamer are positioned inside the AxCeSD cylinder and create four passageways. The location of cellopentaoses in the complex structure suggests that four glucan chains are extruded individually through their own passageway along the dimer interface in a twisted manner. The complex structure also shows that the N-terminal loop, especially residue Lys6, seems to be important for cellulose production, as confirmed by in vivo assay using mutant cells with axcesD gene disruption and N-terminus truncation. Taking all results together, a model of the bacterial terminal complex is discussed.

Development of a New Conversion Process Consisting of Hydrothermal Treatment and Catalytic Reaction Using ZrO2-FeO (X) Catalyst to Convert Fermentation Residue into Useful Chemicals
Satoshi Funai, Yumi Satoh, Yasuharu Satoh, Kenji Tajima, Teruoki Tago, Takao Masuda
TOPICS IN CATALYSIS, 53, 7-10, 654, 658, SPRINGER/PLENUM PUBLISHERS, Jun. 2010, [Peer-reviewed]
English, Scientific journal, Conversion of coliform-fermented residue to useful chemicals such as ketone was carried out by a new conversion process consisting of hydrothermal treatment and catalytic reaction using ZrO2-FeO (X) , resulting in the selective production of acetone. Moreover, catalytic reactions using a model compounds were carried out to clarify the reaction mechanism and kinetics for the fermentation residue conversion.

Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS)
Xuerong Han, Yasuharu Satoh, Kenji Tajima, Tokuo Matsushima, Masanobu Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, 6, 517, 523, SOC BIOSCIENCE BIOENGINEERING JAPAN, Dec. 2009, [Peer-reviewed], [Corresponding author]
English, Scientific journal, In our previous paper, we synthesized poly-3-hydroxybutyrate [P(3HB)] by using the water-organic solvent two-phase reaction system (TPRS), in which thiophenyl (R)-3-hydroxybutyrate [ (R)-3HBTP] was used as a precursor of 3HBCoA. We have developed an improved TPRS for the chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA). In this method, acetyl thioester of ethyl thioglycolate (AcETC) was used as a precursor of acetylCoA (AcCoA), which was a donor of CoA. The AcCoA was formed by the ester exchange reaction between CoA in the water phase and AcETC in the organic solvent phase. The AcCoA and free 3-hydroxybutyrate (3HB) in the water phase were converted into 3HBCoA and acetate by a CoA transfer reaction of propionylCoA transferase (PCT). The synthesized 3HBCoA was polymerized sequentially by PHA synthase, and P(3HB) was successfully formed. The maximal yield of P(3HB) was 1.2 g/l under the optimal reaction condition; this is comparable to that of in vivo PHA production. Furthermore, the number of enzymes was reduced and enzyme preparation was simplified by the construction of a fusion protein, PCT-PhaC. The chemo-enzymatic synthesis of P(3HB-co-3-hydroxypropionate) and P(3HB-co-3-mercaptopropionate) was also achieved by the improved TPRS using the fusion protein. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

Chemo-Enzymatic Synthesis of Poly(lactate-co-(3-hydroxybutyrate)) by a Lactate-Polymerizing Enzyme
Kenji Tajima, Yasuharu Satoh, Toshifumi Satoh, Rumi Itoh, Xuerong Han, Seiichi Taguchi, Toyoji Kakuchi, Masanobu Munekata
MACROMOLECULES, 42, 6, 1985, 1989, AMER CHEMICAL SOC, Mar. 2009, [Peer-reviewed]
English, Scientific journal, In our previous paper, we synthesized poly-3-hydroxybutyrate [P(3HB)] by using the water-organic-solvent two-phase reaction system (TPRS), in which (R)-3-hydroxybutyrylCoA [ (R)-3HBCoA] was continuously supplied to PHA synthase by the ester exchange reaction between CoA and thiophenol in thiophenyl (R)-3HB [ (R)-3HBTP]. By applying TPRS to the screening, we found a lactate- (LA-) polymerizing enzyme from PHA synthases. The enzyme was an engineered PHA synthase, which stereoselectively copolymerized (R)-LA together with (R)-3HB. NMR and GPC revealed that the TPRS successfully synthesized poly(lactate-co-(3-hydroxybutyrate)) [P(LA-co-3HB)] using the LA-polymerizing enzyme as a catalyst. The molar ratios of LA in the copolymers were controllable in the range of 0 to 36 mol% by varying the ratio of (R)-LATP and (R)-3HBTP fed into the TPRS. The number-average molecular weight and the polydispersity of P(36 mol%-co-3HB) were 1.1 x 10(4) and 1.4, respectively. This is the first report on the chemo-enzymatic synthesis of P(LA-co-3HB) by a LA-polymerizing enzyme.

微生物を活用したバイオポリマーへの物質変換
佐藤 康治, 田島 健次
環境技術 38, 14, 18, 2009
Scientific journal

Kinetic Analysis of Engineered Polyhydroxyalkanoate Synthases with Broad Substrate Specificity
Ken'ichiro Matsumoto, Fumi Shozui, Yasuharu Satoh, Kenji Tajima, Masanobu Munekata, Seiichi Taguchi
POLYMER JOURNAL, 41, 3, 237, 240, SOC POLYMER SCIENCE JAPAN, 2009, [Peer-reviewed]
English, Scientific journal

Nonvolatile and Shape-Memorized Bacterial Cellulose Gels Swollen by Poly(ethylene glycol)
Yukari Numata, Kazumi Muromoto, Hidemitsu Furukawa, Jian Ping Gong, Kenji Tajima, Masanobu Munekata
POLYMER JOURNAL, 41, 7, 524, 525, SOC POLYMER SCIENCE JAPAN, 2009, [Peer-reviewed]
English, Scientific journal

A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme
Seiichi Taguchi, Miwa Yamadaa, Ken'ichiro Matsumoto, Kenji Tajima, Yasuharu Satoh, Masanobu Munekata, Katsuhiro Ohno, Katsunori Kohda, Takashi Shimamura, Hiromi Kambe, Shusei Obata
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105, 45, 17323, 17327, NATL ACAD SCIENCES, Nov. 2008, [Peer-reviewed]
English, Scientific journal, Polylactate (PLA) is synthesized as a representative bio-based polyester by the chemo-bio process on the basis of metal catalyst-mediated chemical polymerization of lactate (LA) supplied by microbial fermentation. To establish the one-step microbial process for synthesis of LA-based polyesters, we explored whether polyhydroxyalkanoate (PHA) synthase would exhibit polymerizing activity toward a LA-coenzyme A (CoA), based on the fact that PHA monomeric constituents, especially 3-hydroxybutyrate (3HB), are structurally analogous to LA. An engineered PHA synthase was discovered as a candidate by a two-phase in vitro polymerization system previously developed. An LA-CoA producing Escherichia coli strain with a CoA transferase gene was constructed, and the generation of LA-CoA was demonstrated by capillary electrophoresis/MS analysis. Next, when the engineered PHA synthase gene was introduced into the resultant recombinant strain, we confirmed the one-step biosynthesis of the LA-incorporated copolyester, P(6 mol% LA-co-94 mol% 3HB), with a number-average molecular weight of 1.9 x 10(5), as revealed by gel permeation chromatography, gas chromatography/MS, and NMR.

Regulation of endoglucanase gene (cmcax) expression in Acetobacter xylinum
Shin Kawano, Kenji Tajima, Hiroyuki Kono, Yukari Numata, Hitomi Yamashita, Yasuharu Satoh, Masanobu Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106, 1, 88, 94, SOC BIOSCIENCE BIOENGINEERING JAPAN, Jul. 2008, [Peer-reviewed]
English, Scientific journal, Although cellulose is the most abundant biopolymer in nature, the detailed mechanisms of cellulose biosynthesis remain unknown. Acetobacter xylinum is one of the best-studied model organisms for cellulose biosynthesis. Interestingly, the over-expression of the cmcax gene cause enhancement of cellulose production in A. xylinum, while its product (CMCax) has cellulose degradation activity. The addition of CMCax into medium also promotes cellulose production, suggesting that CMCax is involved in cellulose synthetic pathway. In the present study, we reveal the regulation mechanism of cmcax expression in A. xylinum. First, we treated cells with four kinds of beta-glucodisaccharide. Using an enzyme assay and real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), we observed an increase in CMCax activity and an induction of cmcax expression by gentiobiose treatment. Therefore, we concluded that gentiobiose induced cmcax expression. Although gentiobiose does not originally exist in the cultivation medium, we have revealed that membrane and intra-cellular proteins extracted from A. xylinum produce gentiobiose from glucose, which is one of the components in the cultivation medium. Furthermore, we confirmed that cmcax expression in a wild-type strain increased gradually after 5 d cultivation using real-time qRT-PCR. These results have led us to conclude that the increase in cmcax expression after 5 d cultivation is caused by the increase in gentiobiose, which could be synthesized by a condensation reaction in A. xylinum. Since CMCax plays a pivotal role in the cellulose production system, our results will contribute to the elucidation of mechanisms of cellulose biosynthesis.

Purification, Crystallization and Preliminary X-Ray Studies of AxCesD Required for Efficient Cellulose Biosynthesis in Acetobacter xylinum
SongQing Hu, YongGui Gao, Kenji Tajima, Min Yao, Takanori Yoda, Daisuke Shimura, Yasuharu Satoh, Shin Kawano, Isao Tanaka, Masanobu Munekata
PROTEIN AND PEPTIDE LETTERS, 15, 1, 115, 117, BENTHAM SCIENCE PUBL LTD, Jan. 2008, [Peer-reviewed]
English, Scientific journal, AxCesD protein required for bacterial cellulose biosynthesis in Acetobacter xylinum was overexpressed in E. coli, purified and crystallized. Single crystals of SeMet-substituted AxCesD were obtained by the sitting-drop vapor-diffusion method. The crystal belongs to the primitive trigonal space group P3(2), with unit-cell parameters a = b = 77.7 angstrom, and c = 213.9 angstrom. The asymmetric unit in the crystal was assumed to contain 8 protein molecules giving the Matthews coefficient (V(M)) of 2.54 angstrom(3) Da(-1). Se-MAD data were collected to 2.3 angstrom resolution using synchrotron radiations.

In vitro growth and differentiated activities of human periodontal ligament fibroblasts cultured on salmon collagen gel
Nobuhiro Nagai, Kazuo Mori, Yasuharu Satoh, Noritaka Takahashi, Shunji Yunoki, Kenji Tajima, Masanobu Munekata
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 82A, 2, 395, 402, WILEY-LISS, Aug. 2007, [Peer-reviewed]
English, Scientific journal, Marine-derived collagen is expected to be a much safer alternative to calf collagen, which in medical applications carries the risk of bovine spongiform encephalopathy. In this study, acid-soluble collagen was extracted from salmon skin and crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide during fibril formation to produce a crosslinked salmon collagen (SC) gel. The growth rates and the differentiated functions of human periodontal ligament fibroblasts (HPdLFs) cultured on the SC gel were investigated. Growth was faster on the SC gel than on porcine collagen (PC) gel. In addition, the HPdLFs cultured on the SC gel exhibited higher alkaline phosphatase (ALP) activity than those cultured on the PC gel. Quantitative RT- PCR revealed higher mRNA expression of type I collagen, ALP, and osteocalcin in the HPdLFs cultured on the SC gel. HPdLFs had a flat shape on the SC gel and a spindle shape on the PC gel, as revealed by observation with scanning electron microscopy and immunostaining with cytoskeletal protein and vinculin. The results showed that HPdLFs could grow and show highly differentiated activity on the SC gel as well as on the PC gel. (c) 2007 Wiley Periodicals, Inc.

Preparation of salmon atelocollagen fibrillar gel and its application to the cell culture
Nobuhiro Nagai, Shunji Yunoki, Takeshi Suzuki, Yasuharu Satoh, Kenji Tajima, Masanobu Munekata
CELL TECHNOLOGY FOR CELL PRODUCTS, 321, +, SPRINGER, 2007, [Peer-reviewed]
English, International conference proceedings, Salmon atelocollagen (SAC) has not been used as biomaterials due to its low denaturation temperature (19 degrees C). In the present study, we succeeded in preparation of SAC fibrillar gel stable at an actual physical temperature of human by cross-linking during fibril formation, and in cultivating human periodontal ligament cells on the cross-linked SAC fibrillar gel.

Structural characterization of the Acetobacter xylinum endo-beta-1,4-glucanase CMCax required for cellulose biosynthesis
Yoshiaki Yasutake, Shin Kawano, Kenji Tajima, Min Yao, Yasuharu Satoh, Masanobu Munekata, Isao Tanaka
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 64, 4, 1069, 1077, WILEY, Sep. 2006, [Peer-reviewed]
English, Scientific journal, Previous studies have demonstrated thatendoglucanase is required for cellulose biosynthesis both in bacteria and plants. However, it has yet to be elucidated how the endoglucanases function in the mechanism of cellulose biosynthesis. Here we describe the crystal structure of the cellulose biosynthesis-related endo-beta-1,4-glucanase (CMCax; EC 3.2.1.4) from the cellulose-producing Gram-negative bacterium, Acetobacter xylinum (= Gluconacetobacter xylinus), determined at 1.65-angstrom resolution. CMCax falls into the glycoside hydrolase family 8 (GH-8), and the structure showed that the overall fold of the CMCax is similar to those of other glycoside hydrolases belonging to GH-8. Structure comparison with Clostridium thermocellum Ce1A, the best characterized GH-8 endoglucanase, revealed that sugar recognition subsite +3 is completely missing in CMCax. The absence of the subsite +3 leads to significant broadness of the cleft at the cellooligosaccharide reducing-end side. CMCax is known to be a secreted enzyme and is present in the culture medium. However, electron microscopic analysis using immunostaining clearly demonstrated that a portion of CMCax is localized to the cell surface, suggesting a link with other known membrane-anchored endoglucanases that are required for cellulose biosynthesis.

Activities of MC3T3-E1 cells cultured on gamma-irradiated salmon atelocollagen scaffold
Nobuhiro Nagai, Takafumi Anzawa, Yasuharu Satoh, Takeshi Suzuki, Kenji Tajima, Masanobu Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 101, 6, 511, 514, SOC BIOSCIENCE BIOENGINEERING JAPAN, Jun. 2006, [Peer-reviewed]
English, Scientific journal, We investigated the effect of gamma-irradiation on the activities of MC3T3-E1 cells cultured on gamma-irradiated salmon atelocollagen (SAC) scaffolds. SAC-cultured samples were irradiated at doses of 10, 15, and 25 kGy. gamma-Irradiation had a significant impact on the activities of MC3T3-E1 cells. The proliferation rates and alkaline phosphatase activities of MC3T3-E1 cells increased with gamma-irradiation dose.

Enzymatic synthesis and direct observation of poly[ (R)-3-hydroxybutyrate] utilizing hetero-subunit form PHA synthases by atomic force microscopy　　　　　　　　　　　　　　　
Masayuki Minato, Shun Satoh, Takeharu Tsuge, Yasuharu Satoh, Kenji Tajima, Masanobu Munekata
Polymer Preprints, Japan, 55, 2, 5572, 2006
Japanese, International conference proceedings, PHA synthases from Allochromatium vinosum (PhaECAv) and Bacillus sp. INT005 (PhaRCBs) were purified from recombinant bacteria and were used for in vitro poly[ (R)-3-hydroxybutyrate] [P(3HB)] polymerization on highly oriented pyrolytic graphite (HOPG) substrate. After polymerization and subsequent vacuum drying, the surface of the HOPG substrate was observed by atomic force microscopy (AFM). Many granular and fibrillary entities, which are assumed to be PHA synthase and P(3HB), respectively, were observed.

Development of biomaterials using marine-derived salmon collagen　　　　　　　　　　　　　　　
Nobuhiro Nagai, Shunji Yunoki, Kazuo Mori, Toshiyuki Kanayama, Yasuharu Satoh, Kenji Tajima, Masanobu Munekata
Polymer Preprints, Japan, 55, 2, 4859, 2006
Japanese, International conference proceedings, We successfully prepared the thermostable scaffolds, such as a porous sponge, fibrillargel, and elastic gel, from salmon atelocollagen (SC) using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide cross-linking. We applied these cross-linked SC scaffolds to the in vitro cell culture of human periodontal ligament cells. As a result, the attachment, growth, and alkaline phosphatase activities of the cells cultured on the SC scaffolds were the same or more than those on the porcine atelocollagen scaffolds. The SC scaffolds could serve as well as the traditional collagen as a scaffold for an in vitro cell culture and tissue engineering.

Chemoenzymatic synthesis of P(3HB) in a water-organic solvent two-phase reaction system　　　　　　　　　　　　　　　
Ayako Ishii, Koji Nakazawa, Kenji Tajima, Yasuharu Satoh, Toshifumi Satoh, Toyoji Kakuchi, Masanobu Munekata
Polymer Preprints, Japan, 55, 1, 2265, 2006
Japanese, International conference proceedings, We have succeeded in developing of a new method, water-organic solvent two phase reaction system, for in vitro poly(3-hydroxybutyrate) (P(3HB)) synthesis with coenzyme A (CoA) recycling. In the system, thiophenyl (R)-3-hydroxybutyrate was used as a precursor for the monomer, 3-hydroxybutyrylCoA ((R)-3HBCoA). However, (R)-3HB is very expensive
therefore, we synthesized P(3HB) from an inexpensive start material. Thiophenyl 3-keto-butyrate was synthesized, then dissolved in the organic solvent phase. CoA, NADPH, 3-keto-acylCoA reductase (PhaB), and PHA synthase (PhaC) were dissolved in the water phase. 3-keto-butyrylCoA formed was reduced by PhaB, and the (R)-3HBCoA synthesized was polymerized sequentially by PhaC. The polymerization reaction mixture showed signs of turbidity with the progress of the reaction, after which white precipitates formed. The structural analyses by 1H-NMR spectroscopy indicated that the product was P(3HB).

Enzymatic synthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) with CoA recycling using polyhydroxyalkanoate synthase and acyl-CoA synthetase
Y Satoh, F Murakami, K Tajima, M Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99, 5, 508, 511, SOC BIOSCIENCE BIOENGINEERING JAPAN, May 2005, [Peer-reviewed]
English, Scientific journal, We succeeded in developing a novel method for in vitro poly(3-hydroxybutyrate-co-4-hydroxy-butyrate) [P(3HB-co-4HB)] synthesis with CoA recycling using polyhydroxyalkanoate synthase and an acyl-CoA synthetase. Using this method, the monomer compositions in P(3HB-co-4HB)s could be controlled strictly by the ratios of the monomers in the reaction mixtures.

Crystallization and preliminary crystallographic analysis of the cellulose biosynthesisrelated protein CMCax from Acetobacter xylinum
S Kawano, Y Yasutake, K Tajima, Y Satoh, M Yao, Tanaka, I, M Munekata
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 61, Pt 2, 252, 254, BLACKWELL PUBLISHING, Feb. 2005, [Peer-reviewed]
English, Scientific journal, The cellulose biosynthesis-related protein CMCax from Acetobacter xylinum was overexpressed in Escherichia coli, purified and crystallized. Single crystals of selenomethionine (SeMet) substituted CMCax were obtained by the hanging-drop vapour-diffusion method at 293 K, primarily using polyethylene glycol 4000 as a precipitant. The crystals belong to the primitive hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 89.1, c = 94.2 A. The predicted Matthews coefficient (V-M) value is 3.0 angstrom(3) Da(-1) for one CMCax monomer in the asymmetric unit. A single-wavelength anomalous dispersion (SAD) data set was collected to a resolution of 2.3 angstrom using synchrotron radiation.

A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel
N Nagai, S Yunoki, Y Satoh, K Tajima, M Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 98, 6, 493, 496, SOC BIOSCIENCE BIOENGINEERING JAPAN, Dec. 2004, [Peer-reviewed]
English, Scientific journal, We prepared a cell sheet by using collagenase treatment to digest salmon atelocollagen fibrillar gel (SAC gel) on which human periodontal ligament (HPDL) cells had been cultured. The SAC gel was found to be digested completely within 2 h at a concentration of 50 U of collagenase per mg of collagen. The SAC gel on which HPDL cells were cultured for 10 d was treated with collagenase, resulting in the formation of a detached and shrunken cell sheet. Immunostaining results showed that the cytoskeleton and fibronectin matrix level of the cell sheet were maintained after collagenase treatment. In addition, collagenase treatment had almost no effect on the activities of HPDL Cells.

Chemoenzymatic synthesis of poly(3-hydroxybutyrate) in a water-organic solvent two-phase system
K Tajima, Y Satoh, K Nakazawa, H Tannai, T Erata, M Munekata, M Kamachi, RW Lenz
MACROMOLECULES, 37, 12, 4544, 4546, AMER CHEMICAL SOC, Jun. 2004, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, We succeeded in developing of a new method for in vitro poly(3-hydroxybutyrate) (P(3HB)) synthesis with coenzyme A (CoA) recycling. Thiophenyl (R)-3-hydroxybutyrate was used as a precursor for the monomer, (R)-3-hydroxybutylCoA ((R)-3HBCoA). The (R)-3HBCoA was synthesized by the ester exchange reaction between thiophenyl (R)-3-hydroxybutyrate and CoA. Although the polymerization reaction in the buffer (water phase) was performed, the reaction did not occurred. The results of a PHA synthase assay in the presence of thiophenol suggested that the reaction was inhibited by a small amount of thiophenol. To avoid the inhibition of PHA synthase by thiophenol, a water-organic solvent two-phase reaction system was developed for the polymerization reaction. The water-organic solvent two-phase reaction system has been used previously for the bioconversion of substrates with low solubility in water; it was used to remove thiophenol released during the ester exchange reaction from the water phase. Thiophenyl (R)-3-hydroxybutyrate and PHA synthase were dissolved in the organic solvent and water phases, respectively. Ester exchange between thiophenol and CoA occurs at the interface of water and the organic solvent phase, and the (R)-3HB 3-hydroxybutylCoA synthesized was polymerized sequentially by PhaC. The organic solvents with high a logP(ow) of more than 3.9 such as hexane, octane, decane, or dodecane were found to be suitable for the organic solvent phase in the two-phase system. The polymerization reaction mixture showed signs of turbidity in 2 h, after which white precipitates formed. The structural analyses by H-1 NMR spectroscopy indicated that the product was P(3HB). The weight-averaged molecular weight and molecular weight distribution of P(3HB) synthesized were found to be 1.6 x 10(6) and 2.9, respectively. The maximum yield of P(3HB) based on thiophenyl (R)-3-hydroxybutyrate was 47%.

Application of cross-linked salmon atelocollagen to the scaffold of human periodontal ligament cells
N Nagai, S Yunoki, T Suzuki, M Sakata, K Tajima, M Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97, 6, 389, 394, SOC BIOSCIENCE BIOENGINEERING JAPAN, Jun. 2004, [Peer-reviewed]
English, Scientific journal, The purpose of this study was to investigate the application of salmon atelocollagen (SAC) to a scaffold. SAC has a low denaturation temperature and needs to be cross-linked before being used as a scaffold. In the present study, SAC was cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) or dehydrothermal treatment (DHT). The material properties (degree of cross-linking and solubility in phosphate-buffered saline) of the SAC scaffolds crosslinked by EDC (EDC-SAC) and DHT (DHT-SAC) were evaluated. It was found that EDC-SAC had a high degree of cross-linking and high stability compared with DHT-SAC. Human periodontal ligament (HPDL) cells were cultured in the scaffolds for 2 weeks in vitro, and the activities (proliferation rate and alkaline phosphatase [ALP] activity) of HPDL cells cultured in EDC-SAC and DHT-SAC were compared with those cultured in bovine atelocollagen (BAC) scaffolds crosslinked by EDC (EDC-BAC) and DHT (DHT-BAC), respectively. The proliferation rate of HPDL cells cultured in EDC-SAC was equivalent to that in EDC-BAC, and the ALP activity in EDC-SAC was found to be significantly higher than that in EDC-BAC. In the cross-linking by DHT, the cell proliferation rate and the ALP activity in DHT-SAC were lower than those in DHT-BAC. DHT seemed to provide insufficient cross-linking, and DHT-SAC was found to be breakable and contractile, resulting in less cell activity. In contrast, there was no difference in the thermal stability, porous structure, and cell proliferation rate between EDC-SAC and EDC-BAC. In addition, the collagen helix of EDC-SAC was found to be partially denatured, and this structure resulted in the enhancement of ALP activity of HPDL cells compared with that using EDC-BAC. In conclusion, our results indicate that EDC-SAC could be used as a scaffold for in vitro culture.

Enzyme-catalyzed poly(3-hydroxybutyrate) synthesis from acetate with CoA recycling and NADPH regeneration in vitro
Y Satoh, K Tajima, H Tannai, M Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 95, 4, 335, 341, SOC BIOSCIENCE BIOENGINEERING JAPAN, Apr. 2003, [Peer-reviewed]
English, Scientific journal, We established a novel enzyme-catalyzed poly(3-hydroxybutyrate) [P(3HB)] synthesis system capable of recycling CoA on the basis of the P(3HB) biosynthetic pathway in Ralstonia eutropha. The system includes purified beta-ketothiolase (PhaA), NADPH-dependent acetoacetyl-CoA reductase (Phabeta), PHA synthase (PhaC), acetyl-CoA synthetase (Acs) and glucose dehydrogenase (GDH). In this system, acetyl-CoA was synthesized from acetate and CoA by Acs and ATP, and then two molecules of acetyl-CoA were condensed by PhaA to synthesize acetoacetyl-CoA, which was converted to (R)-3-hydroxybutyryl-CoA (3HBCoA) by PhaB and NADPH. The 3HBCoA was polymerized by PhaC and converted to P(3HB). In this system, the CoA molecules that were released during the condensation and polymerization reactions catalyzed by PhaA and PhaC, respectively, were reused successfully for the synthesis of acetyl-CoA. In addition, NADPH, which was consumed in the reduction of acetoacetyl-CoA, was regenerated by the action of GDH. In this system, the yield of P(3HB) synthesized from acetate as the substrate was 5.6 mg in a 5-ml reaction mixture, and the weight-average molecular weight and polydispersity were 6.64x10(6) and 1.36, respectively. Furthermore, CoA was reused at least 26 times, and NADPH was also regenerated at least 26 times during 24 h of reaction.

Synthesis of poly(3-hydroxybutyrate) by immobilized poly(3-hydroxybutyrate) synthase
K Tajima, H Tannai, Y Satoh, M Munekata
POLYMER JOURNAL, 35, 4, 407, 410, SOC POLYMER SCIENCE JAPAN, 2003, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Isolation and characterization of Bacillus sp INT005 accumulating polyhydroxyalkanoate (PHA) from gas field soil
K Tajima, T Igari, D Nishimura, M Nakamura, Y Satoh, M Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 95, 1, 77, 81, SOC BIOSCIENCE BIOENGINEERING JAPAN, Jan. 2003, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, A gram-positive bacterium (designated strain INT005) that accumulated polyhydroxyalkanoate (PHA) was isolated from gas field soil. From its morphological and physiological properties and the partial nucleotide sequence (about 500 bp) of its 16S rDNA, it was suggested that strain INT005 was similar to several species of the genus Bacillus. We confirmed that strain INT005 is a Bacillus sp. The PHA productivities of strain INT005 were higher than those of Bacillus megaterium and Ralstonia eutropha at 37-45degreesC reported to date, and it was suggested that the PHA synthase of INT005 may exhibit moderate thermostability. The bacterium had the ability to produce poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate), and poly(3-hydroxybutyrate-co-6-hydroxyhexanoate-co-3-hydroxyhexanoate) from the appropriate carbon sources. The PHA synthase from INT005 showed similar substrate specificity to those of class I and III PHA synthases and strain INT005 produced PHAs with various monomer compositions. From the analysis of monomer composition and PHA accumulation in the presence of acrylic acid, it was suggested that de novo fatty acid synthesis and P-oxidation are involved in the PHA synthesis of Bacillus sp. INT005. Since Bacillus sp. INT005 could synthesize PHA even at 45degreesC and PHAs with various monomer compositions, and only one report on the cloning of the synthesis-related genes from a Bacillus species (B. megaterium) has been published; Bacillus sp. INT005 is thought to be very valuable source of PHA synthesis-related genes.

Cloning of cellulose synthesis related genes from Acetobacter xylinum ATCC23769 and ATCC53582: Comparison of cellulose synthetic ability between strains
S Kawano, K Tajima, Y Uemori, H Yamashita, T Erata, M Munekata, M Takai
DNA RESEARCH, 9, 5, 149, 156, UNIVERSAL ACADEMY PRESS INC, Oct. 2002, [Peer-reviewed], [Corresponding author]
English, Scientific journal, About 14.5 kb of DNA fragments from Acetobacter xylinum. ATCC23769 and ATCC53582 were cloned, and their nucleotide sequences were determined. The sequenced DNA regions contained endo-beta-1,4-glucanase, cellulose complementing protein, cellulose synthase subunit AB, C, D and beta-glucosidase genes. The results from a homology search of deduced amino acid sequences between A. xylinum ATCC23769 and ATCC53582 showed that they were highly similar. However, the amount of cellulose production by ATCC53582 was 5 times larger than that of ATCC23769 during a 7-day incubation. In A. xylinum ATCC53582, synthesis of cellulose continued after glucose was consumed, suggesting that a metabolite of glucose, or a component of the medium other than glucose, may be a substrate of cellulose. Oil the other hand, cell growth of ATCC23769 was twice that of ATCC53582. Glucose is the energy source in A. xylinum as well as the substrate of cellulose synthesis, and the metabolic pathway of glucose in both strains may be different. These results suggest that the synthesis of cellulose and the growth of bacterial cells are contradictory.

Polyhydroxyalkanoate synthase from Bacillus sp INT005 is composed of PhaC and PhaR
Y Satoh, N Minamoto, K Tajima, M Munekata
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 94, 4, 343, 350, SOC BIOSCIENCE BIOENGINEERING JAPAN, Oct. 2002, [Peer-reviewed]
English, Scientific journal, A polyhydroxyalkanoate (PHA) biosynthesis gene locus from Bacillus sp. INT005 strain, which had been isolated from a gas field, was cloned and analyzed at the molecular level. We found that a 3.8-kbp DraI-digested fragment of genomic DNA of Bacillus sp. INT005 conferred PHA-producing ability to Escherichia coli, which was PHA-negative. The DNA fragment contained three genes, phaR, -B and -C. The activity of 3-ketoacyl-CoA reductase with NADPH was detected in the lysate from recombinant E. coli carrying the phaB gene. Although PHA synthase activity could be detected in the extract from E. coli carrying phaR, -B and -C genes, no such activity could be detected in that from E. coli carrying only the phaC gene. However, the mixture of the crude extracts of E. coli expressing phaR or phaC revealed very high PHA synthase activity. Furthermore, when His-tagged PhaC was purified by Ni-affinity chromatography from the mixture of crude extracts containing His-tagged PhaC or native PhaR, the eluate contained His-tagged PhaC and native PhaR. On the other hand, PhaR did not bind to the column directly. This purified PhaC with PhaR had 160-fold higher specific activity of PHA synthase than that without PhaR. In addition, the kinetics of the purified PhaC with PhaR revealed a lag phase that preceded the linear phase. It has been known that class III PHA synthase is composed of two different subunits, PhaC and PhaE, and phaC and phaE genes are directly linked in the genomes. Furthermore, the PHA synthase has no lag phase. We hence concluded that the PHA synthase of Bacillus sp. INT005 consists of PhaC and PhaR, and has characteristics different from class III PHA synthase.

Effects of endogenous endo-beta-1,4-glucanase on cellulose biosynthesis in Acetobacter xylinum ATCC23769
S Kawano, K Tajima, H Kono, T Erata, M Munekata, M Taka
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 94, 3, 275, 281, SOC BIOSCIENCE BIOENGINEERING JAPAN, Sep. 2002, [Peer-reviewed], [Corresponding author]
English, Scientific journal, Endo-beta-1,4-glucanase (CMCax; EC 3.2.1.4) from Acetobacter xylinum ATCC23769 was expressed as a 6xHis-tagged fusion protein in Escherichia coli. The optimal temperature, pH, K. and V-max of the purified His-tagged CMCax toward carboxymethyl cellulose were 50degreesC, 4.5, 20 mg/ml and 37.2 muM/min, respectively. The number of recognition residues of cello-oligosaccharide by this enzyme were five (cellopentaose) or longer, and the stereochemical course of hydrolysis was of the inverting type. Addition of a small amount (1.5 mg/l) of His-tagged CMCax into a culture medium enhanced cellulose production 1.2-fold. CMCax overproduction in A. xylinum also enhanced the yield of cellulose production. Transmission electron microscopic analysis revealed that the cellulose ribbons secreted from the CMCax overproducing strain were dispersed compared with those from the wild type strain in the same manner as by carboxymethyl cellulose addition. These results could suggest that CMCax from A. xylinum influences in cellulose ribbon assembly, which is considered to be a rate-determined process in cellulose synthesis.

Cloning and sequencing of the beta-glucosidase gene from Acetobacter xylinum ATCC 23769
K Tajima, K Nakajima, H Yamashita, T Shiba, M Munekata, M Takai
DNA RESEARCH, 8, 6, 263, 269, UNIVERSAL ACADEMY PRESS INC, Dec. 2001, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, The beta-glucosidase gene (bglxA) was cloned from the genomic DNA of Acetobacter xylinum ATCC 23769 and its nucleotide sequence (2200 bp) was determined. This bglxA gene was present downstream of the cellulose synthase operon and coded for a polypeptide of molecular mass 79 kDa. The overexpression of the beta-glucosidase in A. xylinum caused a tenfold increase in activity compared to the wild-type strain. In addition, the action pattern of the enzyme was identified as G3ase activity. The deduced amino acid sequence of the bglxA gene showed 72.3%, 49.6%, and 45.1% identity with the beta-glucosidases from A. xylinum subsp. sucrofermentans, Cellvibrio gilvus, and Mycobacterium tuberculosis, respectively. Based on amino acid sequence similarities, the beta-glucosidase (BglxA) was assigned to family 3 of the glycosyl hydrolases.

Cloning and sequencing of the levansucrase gene from Acetobacter xylinum NCI 1005
K Tajima, T Tanio, Y Kobayashi, H Kohno, M Fujiwara, T Shiba, T Erata, M Munekata, M Takai
DNA RESEARCH, 7, 4, 237, 242, UNIVERSAL ACADEMY PRESS INC, Aug. 2000, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, The levansucrase gene (lsxA) was cloned from the genomic DNA of Acetobacter xylinum NCI 1005, and the nucleotide sequence of the lsxA gene (1,293 bp) was determined. The deduced amino acid sequence of the lsxA gene showed 57.4% and 46.2% identity with the levansucrases from Zymomonas mobilis and Erwinia amylovora, respectively, while only 35.2% identity with that from Acetobacter diazotrophicus. The gene product of lsxA (LsxA) that was overproduced in E. coli coded for a polypeptide of molecular mass 47 kDa. The LsxA released glucose and produced polysaccharide from sucrose, the structure of which was analyzed by nuclear magnetic resonance spectroscopy and determined to be a beta-(2,6)-linked polyfructan. From these results, it can be concluded that the lsxA gene encodes A. xylinum levansucrase.

Structural analyses of new tri- and tetrasaccharides produced from disaccharides by transglycosylation of purified Trichoderma viride beta-glucosidase
H Kono, S Kawano, K Tajima, T Erata, M Takai
GLYCOCONJUGATE JOURNAL, 16, 8, 415, 423, KLUWER ACADEMIC PUBL, Aug. 1999, [Peer-reviewed]
English, Scientific journal, A new beta-glucosidase was partially purified from Trichoderma viride cellulase. This beta-glucosidase catalyzed a transglycosylation reaction of cellobiose to give beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 4)-D-Glc (1, yield: 18.8%) and beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 4)-D-Glc (2, 3.7%), regioselectively. Furthermore, the enzyme regioselectively converted laminaribiose and gentiobiose into beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 3)-D-Glc (3, 15.3%) and beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 6)-D-Glc (4, 20.2%), respectively. The structures (1-4) of the products were determined by H-1 and C-13 NMR spectroscopies. This high regio- and stereoselectively of the beta-glucosidase could be applied for oligosaccharide synthesis.
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The production of a new water-soluble polysaccharide by Acetobacter xylinum NCI 1005 and its structural analysis by NMR spectroscopy
K Tajima, N Uenishi, M Fujiwara, T Erata, M Munekata, M Takai
CARBOHYDRATE RESEARCH, 305, 1, 117, 122, ELSEVIER SCI LTD, Dec. 1997, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, A new water-soluble polysaccharide (WSP) was isolated from a culture of Acetobacter xylinum NCI 1005 grown on sucrose. The structure of the WSP was analysed by nuclear magnetic resonance spectroscopy and determined to be a beta-(2-->6)-linked polyfructan, which is structurally different from the polymer synthesized from glucose instead of sucrose by the same strain. The discovery of this new polysaccharide has revealed that the bacterium is able to synthesize two different kinds of water-soluble polysaccharides. (C) 1998 Elsevier Science Ltd.

Synthesis of two water-soluble polysaccharides by Acetobacter sp. NCI 1005
K Tajima, N Uenishi, M Fujiwara, T Erata, M Munekata, M Takai
MACROMOLECULAR SYMPOSIA, 120, 19, 28, HUTHIG & WEPF VERLAG, Jul. 1997, [Invited]
English, Scientific journal, A new polysaccharide was isolated from the culture of Acetobacter aceti subsp. xylinum (A. xylinum) NCl 1005 grown on sucrose. The structure of the polymer was analyzed by nuclear magnetic resonance (NMR) spectroscopy, The water-soluble polysaccharide (WSP) was determined to be beta(2, 6) linked polyfructan, which was structurally different from the polymer synthesized from glucose instead of sucrose by the same strain. The discovery of the new polysaccharide has elucidated that the bacterium has the ability to synthesize two different kinds of water-soluble polysaccharides.

Enhancement of bacterial cellulose productivity by water-soluble chitosan
K Tajima, M Fujiwara, M Takai, J Hayashi
MOKUZAI GAKKAISHI, 42, 3, 279, 288, JAPAN WOOD RES SOC, 1996, [Peer-reviewed]
English, Scientific journal, Acetobacter aceti subsp. xylinum was cultivated in Hestrin-Schramm (HS) medium containing a water-soluble chitosan oligomer (WSCO) or a water-soluble chitosan derivative (WSCD) to study their effects on bacterial cellulose (BC) productivity. The BC yields in the HS medium with WSCO or WSCD were about 1.3-1.7 times that in normal HS medium. The contents of WSCOs and WSCDs in the bacterial cellulose composites (BCCs) were 0-5(w/w)% and 8-17(w/w)%, respectively. The results of yields, contents and characterizations suggest that WSCO and WSCD enhance BC productivity and that the mechanism of the enhancement are different from each other.

BIOLOGICAL-CONTROL OF CELLULOSE
K TAJIMA, M FUJIWARA, M TAKAI
MACROMOLECULAR SYMPOSIA, 99, 149, 155, HUTHIG & WEPF VERLAG, Sep. 1995, [Invited]
English, Scientific journal, The enhancement of bacterial cellulose (BC) productivity using sucrose as a carbon source has been obtained by the co-cultivation of two different types of acetic acid bacteria BC yields for the given mix ratio of bacteria were larger than that of control. The contents of water-soluble polymer (WSP) in the BC composites (BCCs) are a range of 5-30 wt-%. This will be due to the formation of glucose and fructose through the hydrolysis of sucrose by sucrase secreted from Acetobacter sp.. In addition, this preparation method would be applied to synthesize a new type of BC having both high biodegradability and other functions.

SYNTHESIS OF BACTERIAL CELLULOSE COMPOSITE BY ACETOBACTER-XYLINUM .1. ITS MECHANICAL STRENGTH AND BIODEGRADABILITY
K TAJIMA, M FUJIWARA, M TAKAI, J HAYASHI
MOKUZAI GAKKAISHI, 41, 8, 749, 757, JAPAN WOOD RES SOC, 1995, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, Bacterial cellulose (BC) produced by Acetobacter xylinum (A. xylinum) is a very unique and interesting material, because it naturally has both great mechanical strength and biodegradability. We have succeed in controlling these properties by the incorporation of water-soluble polymers (WSPs), such as carboxymethyl cellulose (CMC), methyl cellulose (MC), and so forth. The incorporation was performed by the incubation of A. xylinum in a standard medium containing a WSP.
Young's modulus of the obtained BC composite (BCC) was about 1-3 times that of normal BC (NBC) (30-40 GPa). For the enzymatic degradation test, BCC(CMC) was degraded completely by cellulase, while only 20-40% of BCC(MC) were degraded. For degradation test in native soil, all BCC samples were degraded considerably at 30 degrees C for 28 days as well as was normal BC (NBC). This production method will be used to synthesize a new type of BC having both excellent biodegradability and other functions.

Enhancement of Bacterial Cellulose Productivity and Preparation of Branched Polysaccharide-Bacterial Cellulose Composite by Co-Cultivation of Acetobacter Species
K. Tajima, H. Ito, M. Fujiwara, M. Takai, J. Hayashi
Sen'i Gakkaishi, 51, 7, 323, 332, 1995, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, We have succeeded in enhancing the bacterial cellulose (BC) productivity using sucrose as a carbon source by the co-cultivation of two different types of Acetobacter species, BC-producing Acetobacter xylinum NCI 1051 and ATCC 10245, which utilize glucose as a carbon source, and branched polysaccharide (BP)-producing Acetobacter aceti subsp. xylinum NCI 1005, which utilizes sucrose as a carbon source. BP-BC composite yields for given mixing ratios of these two species of bacteria were greater than that of control. The contents of BP in the BP-BC composites were 5-30wt%. It was suggested that the BC productivity of Acetobacter xylinum NCI 1051 and ATCC 10245 using sucrose as the carbon source was enhanced by co-cultivation from the increase in the BC production amount. This seems to be due to the formation of glucose and fructose through the hydrolysis of sucrose by sucrase secreted from Acetobacter aceti subsp. xylinum NCI 1005. © 1995, The Society of Fiber Science and Technology, Japan. All rights reserved.

Synthesis and characterization of bacterial cellulose composite (BCC)
Kenji Tajima, Masashi Fujiwara, Mitsuo Takai, Jisuke Hayashi
Proceedings of the International Conference on Cellulose and Cellulose Derivatives: Physico-Chemical Aspects and Industrial Applications, 9, 14, 1993, [Invited]
English

Cellulose production by Acetobacter xylinun in the presence of cellulase
Tsutomu Nakamura, Kenji Tajima, Masashi Fujiwara, Mitsuo Takai, Jisuke Hayashi
Proceedings of the International Conference on Cellulose and Cellulose Derivatives: Physico-Chemical Aspects and Industrial Applications, 3, 8, 1993, [Invited]
English

細菌由来ナノセルロースの経口摂取による腸内細菌叢調節が食事性肥満モデルマウスの体重増加に与える影響の評価　　　　　　　　　　　　　　　
松尾アモリム クリスティーナ菜々, 安藤 英紀, 田島 健次, 高田 春風, 松島 得雄, 草野 貴友, 石田 竜弘, 日本栄養・食糧学会大会講演要旨集, 78回, 257, 257, Apr. 2024
(公社)日本栄養・食糧学会, Japanese

水およびヒドロキシカルボン酸を開始剤に用いたカルボキシ末端脂肪族ポリエステルの簡便合成
徐ハニョン, 高橋逸貴, LI Feng, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

Synthesis and characterization of surface-modified nanofibrillated bacterial cellulose by EDTA for the adsorption of heavy metal ions
佐々涼水, 木下七海, 岸本亮太, 藤田彩華, 甲野裕之, 田島健次, 日本化学会春季年会講演予稿集(Web), 104th, 2024

セルロース由来原料を用いたケミカルリサイクル可能な非天然型多糖類の合成
水上湧太, 筧祐人, LI Feng, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2024, 2024

Development and usage of fully recyclable new material (bacterial produced cellulose nanofibers) using sugar beet
田島健次, てん菜研究会報, 64, 2024

Chemically Recyclable Unnatural Polysaccharides Synthesized from Biomass Resources
LI Feng, 水上湧太, 筧祐人, 田島健次, 山本拓矢, 磯野拓也, 佐藤敏文, 佐藤敏文, 日本化学会春季年会講演予稿集(Web), 104th, 2024

ナノフィブリル化バクテリアセルロースを用いたCHO細胞の浮遊培養における細胞死の抑制と抗体生産性への影響　　　　　　　　　　　　　　　
金子 瑛一郎, 辻崎 晴人, 藤原 政司, 安藤 英紀, 石田 竜弘, 谷 博文, 田島 健次, 日本生物工学会大会講演要旨集, 2023年, 187, 187, Aug. 2023
(公社)日本生物工学会, Japanese

ナノフィブリル化バクテリアセルロースを用いたCHO細胞の浮遊培養における細胞死の抑制と抗体生産性への影響　　　　　　　　　　　　　　　
金子 瑛一郎, 辻崎 晴人, 藤原 政司, 安藤 英紀, 石田 竜弘, 谷 博文, 田島 健次, 日本生物工学会大会講演要旨集, 2023年, 187, 187, Aug. 2023
(公社)日本生物工学会, Japanese

ナノフィブリル化バクテリアセルロースの経口摂取がもたらす腸内細菌叢の多様性変動と食事性肥満の改善　　　　　　　　　　　　　　　
川口 桂乃, 安藤 英紀, 松尾アモリム クリスティーナ菜々, 田島 健次, 長澤 一樹, 松島 得雄, 草野 貴友, 石田 竜弘, 日本栄養・食糧学会大会講演要旨集, 77回, 242, 242, Mar. 2023
(公社)日本栄養・食糧学会, Japanese

In vitro cellulose synthesis using a cellulose synthetase complex
前田佑那, 伊藤冴恵, 高濱良, 高山剛, 藤原政司, 谷博文, 田島健次, 榎原真二, 今井友也, セルロース学会年次大会講演要旨集, 30th, 2023

Whole genome data acquisition and analysis of cellulose synthesis-related genes and proteins in Gluconacetobacter intermedius NEDO-01
永井和生, 芹沢領, 濱西萌生, 荻野千秋, 高濱良, 藤原政司, 谷博文, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

Suppression of mechanical stress in suspension culture of mammalian cell by nanofibrillated bacterial cellulose
金子瑛一郎, 辻崎晴人, 藤原政司, 谷博文, 田島健次, 安藤英紀, 石田竜弘, セルロース学会年次大会講演要旨集, 30th, 2023

Production of Nanofibrillated Bacterial Cellulose (NFBC) from Agro-industrial By-products
高濱良, 芹沢領, 櫻井博章, 荻野千秋, 高山剛, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

Evaluation of Cellulose Structural changes and Na Ions Dynamics in the Crystal Transition to Cellulose II by Low-Temperature NaOH Treatment
久語佑希, 磯野拓也, 藤原政司, 佐藤敏文, 佐藤敏文, 谷博文, 惠良田知樹, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

Tuning of Transgene Expression in Cellulose-synthesizing Bacteria for In Vivo Nanocomposite Production
飯川慎子, 高濱良, 芹沢領, 濱西萌生, 藤原政司, 谷博文, 近藤哲男, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

Preparation of spheroids with HepG2 cells using nanofibrillated bacterial cellulose and evaluation of its drug metabolism
松尾アモリムクリスティーナ奈々, 安藤英紀, 田島健次, 松島得雄, 草野貴友, 石田竜弘, セルロース学会年次大会講演要旨集, 30th, 2023

Characteristics of Nanofibrillated Bacterial Cellulose Sheets Prepared by Vacuum Filtration, Pressure Filtration, and a Cast Method
平野舞, 池田純子, 辻崎晴人, 田島健次, 奥村浩史, 小瀬亮太, セルロース学会年次大会講演要旨集, 30th, 2023

Preparation and mechanical property of the fiber-reinforced composites of biodegradable resins using the surface-modified nanofibrillated bacterial cellulose
横川愛莉, 玉川璃桜, 高谷賢乃介, 岸本亮太, 甲野裕之, 瀬野修一郎, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

Improved Sensitivity of Bioluminescence Assay in a Paper-Based Analytical Device as a Platform
田中楓子, 大山祥大, 石田晃彦, 真栄城正寿, 渡慶次学, 藤原政司, 田島健次, 谷博文, セルロース学会年次大会講演要旨集, 30th, 2023

Polymer Grafting of Nanofibrillated Bacterial Cellulose using a Click Reaction
星野豪大, HASHIM Hamidah Binti, 瀬野修一郎, FENG Li, 可児浩, 藤原政司, 磯野拓也, 山本拓矢, 谷博文, 佐藤敏文, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

Crystal Transition from cellulose I to Cellulose II by Quenching Treatment with a Low Concentration NaOH Solution
久語佑希, 若旅主拓, 磯野拓也, 藤原政司, 佐藤敏文, 佐藤敏文, 谷博文, 惠良田知樹, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

Development of composite fiber materials using nanofibrillated bacterial cellulose
河端唯, 辻崎晴人, LEE Chaehun, 小瀬亮太, 磯野拓也, 藤原政司, 山本拓矢, 佐藤敏文, 佐藤敏文, 谷博文, 田島健次, セルロース学会年次大会講演要旨集, 30th, 2023

An attempt at bio-organic materials (cellulose) production using bacterium in space
田島健次, 田仲昭子, 砂川直輝, 今井友也, 高橋幸子, 齊藤依里, 田仲広明, 五十嵐圭日子, セルロース学会年次大会講演要旨集, 30th, 2023

Improving physical properties of cellulose acetate by adding cellulose acetate-based block copolymers as novel compatibilizer
辻悠希, 勝原哲, 砂川直輝, 五十嵐圭日子, 高橋憲司, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 佐藤敏文, セルロース学会年次大会講演要旨集, 30th, 2023

Construction of Ultrafine Microphase-separated Structure by Biobased Sugar-Terpenoid Amphiphilic Materials
LEE Chaehun, REE Brian J., CHEN Kai, 小牧凌也, 勝原哲, 山本拓矢, 田島健次, CHEN Hsin-Lung, 佐藤敏文, 磯野拓也, シンポジウム「モレキュラー・キラリティー」講演要旨集, 2023 (CD-ROM), 2023

高分子担持型カルボン酸アルカリ金属塩を開環重合触媒に用いたポリエステル類の合成
高橋逸貴, 高木理, 間藤芳允, 田島健次, 山本拓矢, FENG Li, 磯野拓也, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

ポリフルオレン含有特殊構造ブロック共重合体の合成とカーボンナノチューブ可溶化剤としての応用
芦谷万葉, 陳梅儂, 邱ほう誠, 李ほう, 山本拓矢, 田島健次, 佐藤敏文, 磯野拓也, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

ポリ(N-ビニルピロリドン)含有ブロック共重合体を添加剤とした酢酸セルロースの物性改質
小林嵩弥, 勝原哲, 辻悠希, FENG Li, 山本拓矢, 田島健次, 佐藤敏文, 磯野拓也, 化学系学協会北海道支部冬季研究発表会(Web), 2023, 2023

Rotaxane Formation of Multicyclic Polymer in Silicone Rubber Networks
江部陽, 曽我明日香, LI Feng, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, エラストマー討論会講演要旨集, 33rd, 2022

Improvement of physical properties of biodegradable resin with the addition of surface-grafted bacterial cellulose nanofibers
瀬野修一郎, 可児浩, 細川真明, HASHIM Hamidah binti, EMRAN Nur Aisyah Adlin binti, 勝原哲, 二之湯寛子, 辻崎晴人, XIA Xiaochao, 磯野拓也, 山本拓矢, 佐藤敏文, 折原宏, 田島健次, 成形加工シンポジア, 2022 (CD-ROM), 2022

多環状ポリスチレンの合成とエラストマー添加剤としての応用
海老井大和, 間藤芳允, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

相溶化剤としての応用を目指した酢酸セルロース含有ブロック共重合体の合成
辻悠希, 勝原哲, 砂川直輝, 五十嵐圭日子, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

再利用可能な高分子支持型カルボン酸アルカリ金属塩触媒による環状エステル類の開環重合
高橋逸貴, 高木理, 間藤芳允, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2022, 2022

Synthesis and Microphase-separated Structure of Discrete Block Copolymers Consisting of Inorganic Polymer and Oligosaccharide
西村大輝, 田島健次, 磯野拓也, 佐藤敏文, 繊維学会予稿集(CD-ROM), 77, 1, 2022

Orientation changes of gold nanorods depending on polymer brush structure
関澤祐侑, 三友秀之, 与那嶺雄介, 磯野拓也, 田島健次, 佐藤敏文, 居城邦治, ナノ学会大会講演予稿集, 20th, 2022

Design of Hyperbranched Polyester via Ring-opening Alternating Copolymerization of Cyclic Anhydride and Epoxide
鈴木涼太, XIA Xiaochao, XIA Xiaochao, 磯野拓也, 田島健次, 佐藤敏文, 繊維学会予稿集(CD-ROM), 77, 1, 2022

Preparation of cyclosporine A containing nano-fibrillated bacterial cellulose and its application for the treatment of psoriasis
高田春風, 安藤英紀, 田島健次, 清水太郎, 異島優, 松島得雄, 草野貴友, 石田竜弘, セルロース学会年次大会講演要旨集, 29th, 2022

Investigation of crystal transition process from cellulose I to cellulose II by low temperature alkali treatment
久語佑希, 磯野拓也, 惠良田知樹, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 29th, 2022

Studies for mass production of nanofibrillated bacterial cellulose (NFBC) in aerated and stirred bioreactors
上西将斗, 高濱良, 松島得雄, 磯野拓也, 佐藤敏文, 荻野千秋, 田島健次, セルロース学会年次大会講演要旨集, 29th, 2022

Statistical analysis of nano-fibrillated cellulose structures using image processing
細川真明, 瀬野修一郎, 辻崎晴人, 磯野拓也, 佐藤敏文, 折原宏, 田島健次, セルロース学会年次大会講演要旨集, 29th, 2022

Development of compatibilizer for improving physical properties of cellulose acetate
辻悠希, 勝原哲, 砂川直輝, 五十嵐圭日子, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, セルロース学会年次大会講演要旨集, 29th, 2022

Study on surface modification of nano-fibrillated bacterial cellulose and its interaction with resins
甲野裕之, 辻崎晴人, 宇野大樹, 田島健次, セルロース学会年次大会講演要旨集, 29th, 2022

Evaluation of the effect of orally administrated nano-fibrillated bacterial cellulose (NFBC) to regulate construction of gut microbiota and prevent obesity
川口桂乃, 安藤英紀, 田島健次, 長澤一樹, 清水太郎, 異島優, 松島得雄, 草野貴友, 石田竜弘, セルロース学会年次大会講演要旨集, 29th, 2022

Higher-order structure and dispersed state of nano-fibrillated bacterial cellulose
辻崎晴人, 山縣義文, 宮本圭介, 細川真明, 瀬野修一郎, 磯野拓也, 山本拓矢, 佐藤敏文, 折原宏, 田島健次, セルロース学会年次大会講演要旨集, 29th, 2022

Study on surface-selective carboxymethylation of nano-fibrillated bacterial cellulose
甲野裕之, 塚本英樹, 田島健次, セルロース学会年次大会講演要旨集, 29th, 2022

GH8に属するエンドグルカナーゼBcsZの構造機能解析
藤原孝彰, 藤島あゆみ, 中村結衣, 田島健二, 姚閔, 日本結晶学会年会講演要旨集, 2021, 2021

Evaluation of functions of HepG2 cells cultured by three-dimensional culture system using nano-fibrillated bacterial cellulose (NFBC)
赤木俊介, 安藤英紀, 田島健次, 松島得雄, 草野貴友, 清水太郎, 異島優, 石田竜弘, セルロース学会年次大会講演要旨集, 28th, 2021

Studies for development of three-dimensional culture system using nano-fibrillated bacterial cellulose (NFBC)
安藤英紀, 赤木俊介, 田島健次, 清水太郎, 異島優, 松島得雄, 草野貴友, 石田竜弘, セルロース学会年次大会講演要旨集, 28th, 2021

Influence of production conditions of filtration method under reduced pressure on optical properties of nano-fibrillated bacterial cellulose sheet
西本博亮, 松島得雄, 田島健次, 小瀬亮太, セルロース学会年次大会講演要旨集, 28th, 2021

Synthesis and mechanical properties of cellulose acetate-based ABA-type triblock copolymer
勝原哲, 高木靖子, 砂川直輝, 五十嵐圭日子, 北岡本光, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, セルロース学会年次大会講演要旨集, 28th, 2021

In vitro enzymatic polymerization of β-2,6-fructan and thermal and mechanical properties of ester derivatives
岡田征三, 木村聡, 田島健次, 岩田忠久, セルロース学会年次大会講演要旨集, 28th, 2021

Preparation of surface-modified nano-fibrillated bacterial cellulose and its application to fiber-reinforced nanocomposite resins
辻崎晴人, 宇野大輝, 岸本亮太, 甲野裕之, 田島健次, セルロース学会年次大会講演要旨集, 28th, 2021

Surface-modified nano-fibrillated bacterial cellulose by EDTA and its adsorption performance for metal ions
藤田彩華, 木下七海, 岸本亮太, 甲野裕之, 田島健次, セルロース学会年次大会講演要旨集, 28th, 2021

Preparation and functional analysis of terminal complex from Gluconacetobacter using recombinant Escherichia coli
岡睦基, 二之湯寛子, 今井友也, 磯野拓也, 山本拓矢, 佐藤敏文, YAO Min, 田島健次, 日本生物工学会大会講演要旨集, 73rd, 2021

Investigation of crystal transition process from cellulose I to cellulose II by low temperature alkali treatment
久語佑希, 磯野拓也, 田島健次, 佐藤敏文, 惠良田知樹, セルロース学会年次大会講演要旨集, 28th, 2021

Viscoelastic properties of nano-fibrillated bacterial cellulose
辻崎晴人, 山縣義文, 宮本圭介, 細川真明, 瀬野修一郎, 磯野拓也, 山本拓矢, 佐藤敏文, 折原宏, 田島健次, セルロース学会年次大会講演要旨集, 28th, 2021

Structural and functional analysis of terminal complex from Gluconacetobacter using recombinant Escherichia coli
岡睦基, 二之湯寛子, 今井友也, 磯野拓也, 山本拓矢, 佐藤敏文, YAO Min, 田島健次, セルロース学会年次大会講演要旨集, 28th, 2021

Structure and function of cellulose synthase complex from gluconacetobacter
田島健次, 日本生物工学会大会講演要旨集, 73rd, 2021

組換え大腸菌を用いた酢酸菌セルロース合成酵素複合体の調製と結晶構造解析
岡睦基, 二之湯寛子, 今井友也, 磯野拓也, 山本拓矢, 佐藤敏文, 姚閔, 田島健次, 日本結晶学会年会講演要旨集, 2021, 2021

Preparation of Silicone Rubbers with Rotaxane Cross-Linking Comprising of Multicyclic Polymer
江部陽, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 今崎篤, 丸林弘典, 陣内浩司, エラストマー討論会講演要旨集, 32nd, 2021

Construction of Ultrafine Microphase-separated Structure by Sugar-Terpenoid Hybrid Materials
LEE Chaehun, REE Brian J., CHEN Kai, 小牧凌也, 勝原哲, 磯野拓也, 山本拓矢, 田島健次, CHEN Hsin-Lung, 佐藤敏文, シンポジウム「モレキュラー・キラリティー」講演要旨集, 2021, 2021

Development of bio-based elastomer with cellooligosaccaharide derivative as the hard segment
勝原哲, 高木靖子, 砂川直輝, 五十嵐圭日子, 山本拓矢, 田島健次, 磯野拓也, 佐藤敏文, Cellulose Communications, 28, 4, 2021

Development of novel PTX formulations using hydrophilic or amphiphilic nanofibrillated bacterial cellulose and evaluation of their therapeutic efficacy on peritoneally disseminated gastric cancer
赤木俊介, 安藤英紀, 田島健次, 松島得雄, 草野貴友, 清水太郎, 異島優, 石田竜弘, 日本薬剤学会年会講演要旨集(CD-ROM), 36th, 2021

Influence of production conditions of filtration method under reduced pressure on optical and physical properties of nanofibrillated bacterial cellulose sheet
西本博亮, 小瀬亮太, 松島得雄, 田島健次, 繊維学会予稿集(CD-ROM), 76, 1, 2021

バクテリアセルロース合成酵素複合体構成成分BosCの構造解析の試み
佐藤亨, 大内香予子, 今井友也, 田島健次, 尾瀬農之, 姚閔, 日本結晶学会年会講演要旨集, 2020 (CD-ROM), 2020

ポリスチレン-ポリメタクリル酸メチル共重合体のポリスチレン側鎖修飾によるミクロ相分離挙動の制御
田中舜馬, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 54th, 2020

セルロースシートとカルボキシメチルセルロース間の吸着挙動についての分子動力学計算
依田龍輝, 田島健次, 佐藤信一郎, 高分子学会北海道支部研究発表会講演要旨集, 54th, 2020

天然由来原料からなる両親媒性分子による超微細ミクロ相分離構造の構築
李采訓, 小牧凌也, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 54th, 2020

Synchronized Biosynthesis of Hyaluronic acid / bacterial cellulose nanofiber composite directly secreted from Gluconacetobacter
近藤哲男, 高濱良, 加藤歩並, 田川聡美, 田島健次, 日本農芸化学会大会講演要旨集(Web), 2020, 2020

Structure and function of cellulose synthase complex from Gluconacetobacter
田島健次, 日本農芸化学会大会講演要旨集(Web), 2020, 2020

Cellulose Synthesis in an Acetic Acid Bacterium: Closing in on the Secret Behind an Ultra-Precision Nanomachine Spinning a Cellulose Nanofiber
田島健次, 今井友也, YAO Min, 化学と生物, 58, 8, 453, 460, 2020, [Invited], [Lead author]
Japanese, Introduction scientific journal

セルロースナノファイバーによる有用材料の開発 4 セルロース合成菌を用いたナノセルロースの合成
田島健次, アグリバイオ, 3, 6, 528‐532, 20 Jun. 2019, [Invited], [Lead author]
Japanese

β‐2,6‐フルクタンの試験管内酵素重合とエステル誘導体の熱物性評価
岡田征三, 木村聡, 榎本有希子, 田島健次, 岩田忠久, 繊維学会予稿集, 74, 1 (CD-ROM), ROMBUNNO.2P260, 05 Jun. 2019
Japanese

セルロース合成菌を用いたナノセルロースの合成
タジマ ケンジ, アグリバイオ = Agricultural biotechnology, 3, 6, 528, 532, Jun. 2019
北隆館, Japanese

バクテリアを用いたナノセルロースの調製とその利用
田島健次, 日本化学会春季年会講演予稿集(CD-ROM), 99th, 2019

Application of nano fibrillated bacterial cellulose (Fibnano) to an anticancer drug for intraperitoneal injection
安藤英紀, 石田竜弘, 田島健次, 松島得雄, Cellulose Communications, 26, 4, 2019

分子内架橋を基盤とした両親媒性ブロック共重合体の水中自己組織化制御法
田中涼斗, 渡部航大, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

有機触媒を用いたメチルグリコリドの位置選択的開環重合
牧野寛, 蛸島薫, 斎藤達也, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

カチオン化によるセルロースナノファイバーの表層改質
藤田彩華, 小林祐乃, 岸本亮太, 甲野裕之, 松島得雄, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

ポリビニルフェロセンとマルトトリオースからなるブロック共重合体の合成
勝原哲, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

セルロースナノファイバー表層上でのEDTAグラフト化
藤田彩華, 木下七海, 岸本亮太, 甲野裕之, 松島得雄, 坂入信夫, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

ヤヌス型分子内架橋ブロック共重合体の合成とミクロ相分離解析
貝沢野矢, 渡部航大, 磯野拓也, 山本拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

トリオールボレート塩モノマーの連鎖重縮合による両末端官能基化ポリフルオレンの精密合成
藤原魁佑, 小林三朗, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

ナノフィブリル化バクテリアセルロースの表層アセチル化
辻崎晴人, 岸本亮太, 藤田彩華, 甲野裕之, 松島得雄, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 53rd, 2019

酢酸菌由来セルロース合成関連蛋白質BcsDのN末端変異株の作製と評価
小池裕之, 永谷良騎, 水野正浩, 天野良彦, 田島健次, SAXENA Inder, 繊維学会予稿集, 74, 2 (CD-ROM), 2019

ナノフィブリル化バクテリアセルロースの表層アセチル化改質
辻崎晴人, 岸本亮太, 藤田彩華, 甲野裕之, 松島得雄, 田島健次, セルロース学会年次大会講演要旨集, 26th, 2019

両親媒性ナノフィブリル化バクテリアセルロース(Fibnano)の放出制御型がん治療製剤への応用
赤木俊介, 安藤英紀, 田島健次, 松島得雄, 草野貴友, 石田竜弘, セルロース学会年次大会講演要旨集, 26th, 2019

ナノフィブリル化バクテリアセルロース添加がパルプ繊維シートの物性に及ぼす影響
平川茂雅, 小瀬亮太, 田島健次, 松島得雄, セルロース学会年次大会講演要旨集, 26th, 2019

遺伝子組換え大腸菌における酢酸菌由来セルロース合成酵素複合体の再構築
二之湯寛子, 青島望乃, 今井友也, 磯野拓也, 山本拓矢, 佐藤敏文, MIN Yao, 田島健次, セルロース学会年次大会講演要旨集, 26th, 2019

酢酸菌由来セルロース合成酵素の抽出および精製
青島望乃, 二之湯寛子, 今井友也, 磯野拓也, 山本拓矢, 佐藤敏文, MIN Yao, 田島健次, セルロース学会年次大会講演要旨集, 26th, 2019

バクテリアセルロースペリクルの構造およびレオロジー特性
沼田ゆかり, 甲野裕之, 森明音, 岸本亮太, 田島健次, セルロース学会年次大会講演要旨集, 26th, 2019

フルーツ由来新奇酢酸菌によるナノセルロースの合成とその応用
田島健次, 小瀬亮太, 松島得雄, 石田竜弘, 安藤英紀, 日本醸造協会誌, 114, 9, 540, 549, 2019, [Invited], [Lead author]
Japanese, Introduction scientific journal

両親媒性ナノフィブリル化バクテリアセルロースを基剤とした放出制御型Paclitaxel製剤の開発
赤木俊介, 安藤英紀, 田島健次, 松島得雄, 草野貴友, 石田竜弘, 日本薬剤学会年会講演要旨集(Web), 34th, 152, 152, 2019
(公社)日本薬剤学会, Japanese

遺伝子導入酢酸菌によるナノセルロース/ヒアルロン酸バイオ複合材料の構築
加藤歩並, 高濱良, 近藤哲男, 田島健次, 日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 69th, ROMBUNNO.K14‐04‐1630, 2019
Japanese

オリゴ糖鎖を一成分とする新規エラストマー材料の設計
磯野拓也, 中平早紀, 間宮弘明, 田島健次, BORSALI Redouane, 佐藤敏文, ポリマー材料フォーラム講演予稿集, 27th, 178, 07 Nov. 2018
Japanese

かご型および多環状構造を有するポリカプロラクトンの系統的合成と物性評価
間藤芳允, 本田康平, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.3D08, 29 Aug. 2018
Japanese

オリゴ糖鎖とポリ(δ‐デカノラクトン)からなる分岐状ブロック共重合体の合成とミクロ相分離挙動
中平早紀, HUNG Chih‐Chien, 磯野拓也, 山本拓矢, 田島健次, CHEN Wen‐Chang, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.3D14, 29 Aug. 2018
Japanese

クリック反応性基を有するセロオリゴ糖の酵素合成とその誘導体化
高木靖子, 磯野拓也, 砂川直輝, 山本拓矢, 五十嵐圭日子, 北岡本光, 佐藤敏文, 田島健次, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.2Pb102, 29 Aug. 2018
Japanese

単分散オリゴ糖‐ソラネソールブロック共重合体によるシングルナノオーダー自己組織化
小牧凌也, 川上菜穂, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1K09, 29 Aug. 2018
Japanese

両親媒性ナノフィブリル化バクテリアセルロース合成における培養条件の最適化
上西将斗, 石井亨, 松島得雄, 甲野裕之, 磯野拓也, 山本拓矢, 佐藤敏文, 田島健次, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1Pe103, 29 Aug. 2018
Japanese

ブロックコポリマーの分子内架橋によるミクロ相分離構造の微細化
渡部航大, 勝原哲, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.2C05, 29 Aug. 2018
Japanese

芳香族カルボン酸塩を触媒に用いたポリ乳酸およびポリトリメチレンカーボネートの精密合成
蛸島薫, 齋藤達也, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.3Pa001, 29 Aug. 2018
Japanese

二重活性化を鍵とする環境低負荷触媒を用いた脂肪族ポリエステル類の合成
齋藤達也, 蛸島薫, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1D05, 29 Aug. 2018
Japanese

発酵ナノセルロースファイバーの表層疎水改質
甲野裕之, 宇野大輝, 辻崎晴人, 岸本亮太, 松島徳雄, 田島健次, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1Pf052, 29 Aug. 2018
Japanese

末端選択的エステル交換反応によるポリスチレン‐ポリメタクリル酸メチルブロック共重合体の末端修飾
吉田康平, 田中舜馬, 宮城賢, 磯野拓也, 山本拓矢, 田島健次, BORSALI Redouane, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.2Pe001, 29 Aug. 2018
Japanese

末端クリック反応性ポリヒドロキシアルカノエートを基盤とした相溶化剤の開発
小山貴史, 齋藤達也, 小林新吾, 大倉徹夫, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1Y06, 29 Aug. 2018
Japanese

トリオールボレート塩型モノマーの鈴木・宮浦触媒移動型連鎖縮合重合によるポリフルオレンの合成
小林三朗, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1Pd006, 29 Aug. 2018
Japanese

セルロース合成菌の同定と得られたペリクルの構造およびレオロジー特性に関する研究
沼田ゆかり, 甲野裕之, 森明音, 岸本亮太, 田島健次, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1Pf072, 29 Aug. 2018
Japanese

β‐2,6‐フルクタンの試験管内酵素重合
岡田征三, 木村聡, 榎本有希子, 岩田忠久, 田島健次, セルロース学会年次大会講演要旨集, 25th, 77, 01 Jul. 2018
Japanese

ナノフィブリル化バクテリアセルロース(Fibnano)を用いた新規パクリタキセル製剤の開発とがん治療への展開
安藤英紀, 望月啓志, 藤田研司, 田島健次, 松島得雄, 草野貴友, 石田竜弘, セルロース学会年次大会講演要旨集, 25th, 41‐42, 01 Jul. 2018
Japanese

バクテリアを用いた両親媒ナノセルロースの合成とその応用
田島健次, 楠元領, 石井亨, 上西将斗, 小瀬亮太, 甲野裕之, 松島得雄, 磯野拓也, 山本拓矢, 佐藤敏文, セルロース学会年次大会講演要旨集, 25th, 35‐36, 01 Jul. 2018
Japanese

ナノフィブリル化バクテリアセルロースの表層疎水化
宇野大輝, 岸本亮太, 甲野裕之, 松島得雄, 磯野拓也, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 25th, 101, 01 Jul. 2018
Japanese

電界紡糸法による配向性樹脂/セルロースナノファイバーの複合材料の創製
石井亨, 松島得雄, BORSALI Redouane, 磯野拓也, 山本拓矢, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 25th, 154, 01 Jul. 2018
Japanese

セロデキストリンホスホリラーゼを用いた末端官能基化セロオリゴ糖の合成およびその誘導体化
高木靖子, 磯野拓也, 砂川直輝, 山本拓也, 五十嵐圭日子, 北岡本光, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 25th, 64, 01 Jul. 2018
Japanese

Synthesis and Large‐scale Production of Nanofibrillated Bacterial Cellulose (NFBC) by Acetobacter
田島健次, 小瀬亮太, 松島得雄, 紙パルプ技術タイムス, 61, 6, 23‐29, 29, Jun. 2018, [Invited]
テックタイムス, Japanese

多環状ポリカプロラクトンの合成と物性解析
間藤芳允, 本田康平, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.2C26, 08 May 2018
Japanese

糖鎖とポリテルペンからなる単分散ブロック共重合体の合成とモルフォロジー解析
磯野拓也, 川上菜穂, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.3Pd004, 08 May 2018
Japanese

ポリプロピレンオキシドとオリゴ糖からなるブロック共重合体の合成とミクロ相分離挙動
高木靖子, 磯野拓也, 田島健次, BORSALI Redouane, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.1E14, 08 May 2018
Japanese

ポリカプロラクトンとマルトトリオースからなる糖鎖スターブロックコポリマーの合成とミクロ相分離挙動
磯野拓也, 川上菜穂, BORSALI Redouane, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.2Pa011, 08 May 2018
Japanese

末端にオリゴ糖鎖を導入したポリスチレン‐ポリメタクリル酸メチルブロック共重合体の合成とミクロ相分離構造
吉田康平, 田中舜馬, 宮城賢, 磯野拓也, 山本拓矢, 田島健次, BORSALI Redouane, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.1E15, 08 May 2018
Japanese

芳香族カルボン酸塩を触媒に用いた環状エステル類の開環重合
蛸島薫, 齋藤達也, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.1D15, 08 May 2018
Japanese

分子内架橋によるブロック共重合体のミクロ相分離構造微細化
勝原哲, 渡部航大, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.1E13, 08 May 2018
Japanese

トリオールボレート塩モノマーの触媒移動型連鎖縮合重合によるポリフルオレンの精密合成
小林三朗, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 1, ROMBUNNO.1F23, 08 May 2018
Japanese

発酵ナノセルロース(NFBC)の大量生産とその医療応用
田島健次, 小瀬亮太, 石田竜弘, 松島得雄, Bio Industry, 35, 4, 55‐63, Apr. 2018, [Invited]
Japanese

Synthesis and self-assembly of poly(styrene-block-methyl methacrylate) end-capped with oligosaccharide
Kenji Tajima, Abstracts of Papers of the American Chemical Society, 255, Mar. 2018
English, Summary international conference

Intramolecular cross-linking of poly(styrene-b-lactide) s for decreasing domain sizes in microphase-separated structures
Kenji Tajima, Abstracts of Papers of the American Chemical Society, 255, Mar. 2018
Summary international conference

ナノファイバーバイオセルロースを用いた新規パクリタキセル製剤の開発
望月啓志, 安藤英紀, 藤田研司, 藤田研司, 田島健次, 異島優, 石田竜弘, 日本薬学会年会要旨集(CD-ROM), 138年会, 4, 73, 73, Mar. 2018
(公社)日本薬学会, Japanese

トリオールボレート塩モノマーの連鎖縮合重合による末端官能基化ポリフルオレンの合成
小林三朗, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 52nd, 32, 15 Jan. 2018
Japanese

ポリプロピレンオキシドとマルトトリオースからなるブロック共重合体の合成とミクロ相分離挙動
高木靖子, 磯野拓也, 田島健次, BORSALI Redouane, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 52nd, 19, 15 Jan. 2018
Japanese

ナノフィブリル化バクテリアセルロースの選択的表層疎水化
宇野大輝, 岸本亮太, 甲野裕之, 松島得雄, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 52nd, 37, 15 Jan. 2018
Japanese

分子内連鎖的環化による多環状ポリマーの合成と物性評価
間藤芳允, 本田康平, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 52nd, 9, 15 Jan. 2018
Japanese

芳香族カルボン酸ナトリウム塩を触媒に用いた環状エステルの開環重合
蛸島薫, 齋藤達也, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2018, ROMBUNNO.1B04 (WEB ONLY), 2018
Japanese

ブロック共重合体の分子内架橋によるミクロ相分離構造の微細化
勝原哲, 渡部航大, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2018, ROMBUNNO.1B02 (WEB ONLY), 2018
Japanese

ソラネソール‐糖鎖ハイブリッド材料によるシングルナノオーダー自己組織化
川上菜穂, REE Brian J, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2018, ROMBUNNO.1B01 (WEB ONLY), 2018
Japanese

オリゴ糖鎖を末端に有するポリスチレン‐ポリメタクリル酸メチルブロック共重合体の合成とミクロ相分離構造
田中舜馬, 吉田康平, 宮城賢, 磯野拓也, 山本拓矢, 田島健次, BORSALI Redouane, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会(Web), 2018, ROMBUNNO.1B03 (WEB ONLY), 2018
Japanese

縮合反応を用いたナノフィブリル化バクテリアセルロースへの機能性タンパク質の導入
梶田悠介, 山本拓矢, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 52nd, 31, 2018
Japanese

ナノフィブリル化バクテリアセルロースのイムノクロマト法への応用
梶田悠介, 松島得雄, 磯野拓也, 山本拓矢, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 25th, 164, 2018
Japanese

t‐Bu‐P₄触媒によるグリシジルエーテルのブロック共重合:トリブロックターポリマーの合成と自己組織化挙動
新宮智樹, BRIAN Ree J, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 67, 2, ROMBUNNO.1D04, 2018
Japanese

Cellulose Synthetic Mechanism in Gluconacetobacter and Production of Nano‐fibrillated Bacterial Cellulose (NFBC) by Aerobic Agitating Cultivation.
小瀬亮太, 松島得雄, 田島健次, Cellulose Communications, 24, 4, 186‐192, 192, Dec. 2017, [Invited]
セルロース学会, Japanese

「セルロースの実験と解析法」シリーズ 第36回 各種セルロースナノファイバーの調製と顕微鏡観察
齋藤継之, 小瀬亮太, 横田慎吾, 田島健次, Cellulose Communications, 24, 4, 203‐206, Dec. 2017, [Invited]
Japanese

酵素処理アオサ抽出デンプンを用いたPHA合成条件の検討
上西将斗, 小林広弥, 清野晃之, 小林孝紀, 藤田伸二, 田島健次, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.1Pd114, 06 Sep. 2017
Japanese

末端にオリゴ糖を有するポリスチレン‐ポリメタクリル酸メチルブロック共重合体の合成およびミクロ相分離構造
吉田康平, 宮城賢, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.2Pd020, 06 Sep. 2017
Japanese

バイオポリエステルとポリカプロラクトンからなるブロック共重合体の合成と相溶化特性
小山貴史, 齋藤達也, 小林新吾, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.1W14, 06 Sep. 2017
Japanese

有機酸塩を触媒に用いた環状エステル類の開環重合
齋藤達也, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.2C01, 06 Sep. 2017
Japanese

糖鎖含有トリブロック共重合体のミクロ相分離に基づいたエラストマー材料の設計
磯野拓也, 中平早紀, BORSALI Redouane, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.1Pf110, 06 Sep. 2017
Japanese

Sub‐10nmのパターニングを実現するポリスチレン‐ポリメタクリル酸メチルブロック共重合体の側鎖変換
吉田康平, TIAN Lin, 宮城賢, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.1L09, 06 Sep. 2017
Japanese

サツマイモデンプン廃液を用いたPHA合成の検討
上西将斗, 清野晃之, 岩波俊介, 田島健次, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.1Pc113, 06 Sep. 2017
Japanese

新規ナノフィブリル化バクテリアセルロース生産菌の取得とその生産性評価
石井亨, 村野玲奈, 大場淳也, 楠元領, 松島得雄, 磯野拓也, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 24th, 51, 01 Jul. 2017
Japanese

バクテリアセルロース複合体ハイドロゲルの構造および物性に関する研究
沼田ゆかり, 甲野裕之, 辻みなと, 田島健次, セルロース学会年次大会講演要旨集, 24th, 56, 01 Jul. 2017
Japanese

ナノフィブリル化バクテリアセルロースの大量生産とその応用
小瀬亮太, 砂川直輝, 田原功太郎, 楠元領, 大場淳也, 松島得雄, 甲野裕之, 磯野拓也, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 24th, 7‐8, 01 Jul. 2017
Japanese

胃がん腹膜播種治療におけるナノファイバーバイオセルロースの応用に関する検討　　　　　　　　　　　　　　　
望月 啓志, 安藤 英紀, 藤田 研司, 田島 健次, 石田 竜弘, 日本DDS学会学術集会プログラム予稿集, 33回, 183, 183, Jun. 2017
日本DDS学会, Japanese

植物と高分子:バイオマス素材から植物科学まで 天然におけるセルロース合成機構の解明を目指したセルロース合成酵素の異宿主発現とin vitroセルロース合成の試み
砂川直輝, 鮫島正浩, 田島健次, 五十嵐圭日子, 高分子, 66, 6, 288‐289, 01 Jun. 2017
Japanese

三つ葉型および四つ葉型ポリマーのトポロジー変換によるカゴ型ポリマーの合成
磯野拓也, 佐藤悠介, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 1, ROMBUNNO.3Pc007, 15 May 2017
Japanese

マルトトリオースとポリ(n‐ブチルアクリレート)からなるABA型トリブロックコポリマーの合成とミクロ相分離挙動
中平早紀, 渡部航大, HUNG Chih‐Chien, 磯野拓也, 山本拓矢, 田島健次, CHEN Wen‐Chang, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 1, ROMBUNNO.1Pe007, 15 May 2017
Japanese

分子内マルチクリック環化によるμ‐ABC三つ葉型ターポリマーの合成
新宮智樹, 佐藤悠介, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 1, ROMBUNNO.3Pd008, 15 May 2017
Japanese

末端クリック反応性バイオポリエステルを原料としたブロック共重合体の合成
小山貴史, 齋藤達也, 小林新吾, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 1, ROMBUNNO.2Pd126, 15 May 2017
Japanese

両親媒性ブロック共重合体の分子内架橋による自己組織化制御
田中涼斗, 渡部航大, 山本拓矢, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 1, ROMBUNNO.3Pc021, 15 May 2017
Japanese

ナノファイバーバイオセルロースの腹腔内がん化学療法への応用に関する検討
望月啓志, 安藤英紀, 藤田研司, 藤田研司, 田島健次, 石田竜弘, 日本薬学会年会要旨集(CD-ROM), 137年会, 4, 92, 92, Mar. 2017
(公社)日本薬学会, Japanese

「セルロースの実験と解析法」シリーズ(第36回)各種セルロースナノファイバーの調製と顕微鏡観察—"Experimental and Analytical Methods of Cellulose" Series : Preparation and microscope observation for some cellulose nanofibers
齋藤 継之, 小瀬 亮太, 横田 慎吾, 田島 健次, Cellulose communications, 24, 4, 203, 206, 2017
セルロース学会, Japanese

The structure analysis of flexible domain of CeSC
野島慎五, 藤島あゆみ, 加藤公児, 加藤公児, 田島健次, 姚閔, 姚閔, 量子ビームサイエンスフェスタ(Web), 2016, 2017

分子内連鎖的環化による種々の多環状ポリマーの合成
本田康平, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 51st, O20, 2017
Japanese

両親媒性ナノフィブリル化バクテリアセルロースの調製とその材料応用
楠元領, 石井亨, 松島得雄, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 51st, O11, 2017
Japanese

ナノセルロース表層上でのシクロデキストリングラフト化とそのゲスト分子認識能
高橋佳奈, 穴井ひかる, 藤田彩華, 甲野裕之, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 51st, P26, 2017
Japanese

ナノセルロースの表層疎水性改質に関する研究
穴井ひかる, 高橋佳奈, 藤田彩華, 甲野裕之, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 51st, P25, 2017
Japanese

新規取得菌によるナノフィブリル化バクテリアセルロースの生産性評価
石井亨, 村野玲奈, 大場淳矢, 楠元領, 松島得雄, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 51st, P44, 2017
Japanese

ポリカプロラクトンとマルトトリオースからなるスターブロックコポリマーの合成とミクロ相分離挙動
川上菜穂, 大塚一世, BORSALI Redouane, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 51st, O10, 2017
Japanese

t‐Bu‐P₄触媒を用いたエポキシドの開環重合による特殊構造トリブロックターポリマーの合成
新宮智樹, 佐藤悠介, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 66, 2, ROMBUNNO.2C03, 2017
Japanese

種々の有機酸塩を触媒に用いた環状エステルの開環重合
相澤佑輔, 齋藤達也, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 2, ROMBUNNO.2G16, 24 Aug. 2016
Japanese

ナノフィブリル化バクテリアセルロースの表面改質とその材料応用
楠元領, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会予稿集(CD-ROM), 65, 2, ROMBUNNO.1Pf100, 24 Aug. 2016
Japanese

核磁気共鳴法によるカルボキシメチルセルロース置換基のコンフォメーション解析
甲野裕之, 岡千夏, 岸本亮太, 藤田彩華, 大島和浩, 橋本久穂, 清水祐一, 田島健次, 高分子学会予稿集(CD-ROM), 65, 2, ROMBUNNO.2Pa039, 24 Aug. 2016
Japanese

極性基を導入したスチレン‐メタクリル酸メチルブロック共重合体のミクロ相分離構造
吉田康平, TIAN Lin, 宮城賢, 磯野拓也, 山本拓矢, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 2, ROMBUNNO.3Pd014, 24 Aug. 2016
Japanese

キメラ酢酸菌によるカードラン/ナノセルロース複合体のin vivo合成
高濱良, 近藤哲男, FANG Ju, 河野信, 田島健次, 化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集, 53rd, 149, 02 Jul. 2016
Japanese

発酵ナノセルロースの表層疎水性改質に関する研究
穴井ひかる, 高橋佳奈, 甲野裕之, 田島健次, セルロース学会年次大会講演要旨集, 23rd, 109, 01 Jul. 2016
Japanese

遺伝子組換えした酢酸菌によるセルロース/カードランバイオナノコンポジットの創製
JU Fang, 田島健次, 河野信, 近藤哲男, セルロース学会年次大会講演要旨集, 23rd, 39‐40, 01 Jul. 2016
Japanese

ナノフィブリル化バクテリアセルロースの分散状態に及ぼすカルボキシメチルセルロースの置換度の影響
楠元領, 大場淳也, 甲野裕之, 磯野拓也, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 23rd, 147, 01 Jul. 2016
Japanese

カルボキシメチルセルロースの化学シフト全帰属とモノマー組成比の決定
甲野裕之, 大島和浩, 橋本久穂, 清水祐一, 田島健次, セルロース学会年次大会講演要旨集, 23rd, 84‐85, 01 Jul. 2016
Japanese

遺伝子組換えによるセルロース合成大腸菌の創製
村野玲奈, 渡辺耕平, 今井友也, 磯野拓也, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 23rd, 114, 01 Jul. 2016
Japanese

発酵ナノセルロース表層でのシクロデキストリングラフト化
高橋佳奈, 穴井ひかる, 甲野裕之, 田島健次, セルロース学会年次大会講演要旨集, 23rd, 108, 01 Jul. 2016
Japanese

末端官能基化星形ポリマーの連鎖的分子内環化によるかご型ポリマーの合成
本田康平, 笹森哲弥, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.2C25, 10 May 2016
Japanese

バクテリアセルロースゲルの表面改質に関する研究
沼田ゆかり, 辻みなと, 甲野裕之, 田島健次, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.1Pa025, 10 May 2016
Japanese

オレフィンメタセシス反応によるジブロックコポリマーの分子内架橋
田中涼斗, 渡部航大, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.1Pf010, 10 May 2016
Japanese

オリゴ糖鎖とポリカプロラクトンからなるスターブロックコポリマーの精密合成とモルフォロジー評価
川上菜穂, 吉田康平, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.2C22, 10 May 2016
Japanese

NMRによるカルボキシメチルセルロースの構造解析~置換基分布とモノマー組成の決定
甲野裕之, 大島和浩, 橋本久穂, 清水祐一, 田島健次, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.2Pf122, 10 May 2016
Japanese

糖鎖含有ポリカプロラクトンと糖類からなるブレンド膜の作製とミクロ相分離構造評価
梅坪優人, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.3Pb044, 10 May 2016
Japanese

α,ω‐ノルボルネン官能基化マクロモノマーの環化重合による多環状ポリマーの合成
磯野拓也, 笹森哲弥, 本田康平, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.2C24, 10 May 2016
Japanese

ポリカプロラクトンとマルトヘプタオースからなるミクトアームスターポリマーの精密合成と水中自己組織化
磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.1Pf004, 10 May 2016
Japanese

P-30 Expression of cellulose synthase in Pichia pastoris
砂川直輝, 鮫島正浩, 五十嵐圭日子, 田島健次, 松永幸大, バイオマス科学会議発表論文集(Web), 11, 2016

末端官能基化スターポリマーの分子内環化によるかご型ポリマーの合成
本田康平, 笹森哲弥, 磯野拓也, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, ROMBUNNO.1B06, 2016
Japanese

マクロモノマーの環化重合による多環状高分子の合成
笹森哲弥, 本田康平, 磯野拓也, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, ROMBUNNO.1B05, 2016
Japanese

マルチクリック環化反応を用いた多環状ポリエーテルの精密合成
松野拓彦, 佐藤悠介, 磯野拓也, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, ROMBUNNO.2B04, 2016
Japanese

ポリメタクリレートからなる分子内架橋ナノ粒子の合成と物性評価
渡部航大, 田中涼斗, 磯野拓也, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2016, ROMBUNNO.P10, 2016
Japanese

セルロースナノファイバー表層へのシクロデキストリングラフト化
高橋佳奈, 穴井ひかる, 甲野裕之, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 50th, P9, 2016
Japanese

トリフルオロアセチル基を有するセルロース誘導体のin vivo合成
村野玲奈, 佐藤悠介, 大場淳矢, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 50th, P28, 2016
Japanese

オリゴ糖鎖とポリカプロラクトンからなる星型ブロックコポリマーの合成
川上菜穂, 吉田康平, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 50th, O21, 2016
Japanese

セルロースナノファイバーの表層疎水改質に関する研究
穴井ひかる, 高橋佳奈, 甲野裕之, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 50th, P7, 2016
Japanese

分子内オレフィンメタセシスを用いた単分子ナノ粒子合成法の確立
渡部航大, 田中涼斗, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 50th, O22, 2016
Japanese

オレフィンメタセシス反応による分子内架橋ブロックコポリマーの合成
田中涼斗, 渡部航大, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 50th, P29, 2016
Japanese

アミノリシス反応によるスチレン‐メタクリル酸メチルブロック共重合体への極性基導入
田林, 吉田康平, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 50th, P30, 2016
Japanese

糖鎖含有ブロック共重合体と糖類からなるブレンド膜の作製とナノ構造評価
梅坪優人, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 50th, P31, 2016
Japanese

両親媒性スターブロックおよびミクトアームスターコポリエーテルの精密合成
佐藤悠介, 宮地香奈, 磯野拓也, 田島健次, 覺知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.1Pg003, 2016
Japanese

開環重合およびマルチクリック環化による多環状ポリエーテルの精密合成
佐藤悠介, 松野拓彦, 磯野拓也, 田島健次, 覺知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.2C23, 2016
Japanese

分子内オレフィンメタセシスによる単分子ナノパーティクルのサイズ選択的合成
渡部航大, 田中涼斗, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 65, 1, ROMBUNNO.2C27, 2016
Japanese

Asaia bogorensis由来AbCeSDのN末端配列がセルロース生産に及ぼす影響
永谷良騎, 水野正浩, 水野正浩, 天野良彦, 田島健次, セルロース学会年次大会講演要旨集, 23rd, 112, 2016
Japanese

バクテリア由来CeSDの構造,機能解析
村野玲奈, 渡辺耕平, 水野正浩, 宇都卓也, 磯野拓也, 湯井敏文, 天野良彦, YAO Min, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 23rd, 115, 2016
Japanese

P-30 Expression of cellulose synthase in Pichia pastoris
SUNAGAWA Naoki, SAMEJIMA Masahiro, IGARASHI Kiyohiko, TAJIMA Kenji, MATSUNAGA Sachihiro, Proceedings of the Conference on Biomass Science, 11, 0, 109, 110, 2016
Cellulose is a main component of plant biomass and it was used as the material, energy source and food source. However, cellulose synthesis process is a complicated system, hence detail is still unknown. In order to solve this issue, we're planning a dynamic visualization of this synthesis process. In this poster, to perform the procedure, we will report on the making of recombinant expression system of plant cellulose synthase (AtCeSA1) and bacterial cellulose synthase (GxCeSAB) in Pichia pastoris., The Japan Institute of Energy, Japanese

【159】触媒移動型重合を介したポリ（３−ヘキシルチオフェン）の定量的末端官能基本化　　　　　　　　　　　　　　　
高島 象一, 中村 亮介, 蓬田 昌伸, 磯野 拓也, 田島 健次, 梶 弘典, 佐藤 敏文, 第５回CSJ化学フェスタ, タワーホール船橋(千葉）, 14 Oct. 2015
Japanese

【158】大環状ポリ（3-ヘキシルチオフェン）の合成と特性評価　　　　　　　　　　　　　　　
高島 象一, 蓬田 昌伸, 磯野 拓也, 田島 健次, 梶 弘典, 佐藤 敏文, 第５回CSJ化学フェスタ, タワーホール船橋(千葉）, 13 Oct. 2015
Japanese

【156】触媒移動型縮合重合を介したポリ (3-ヘキシルチオフェン) の定量的末端官能基化　　　　　　　　　　　　　　　
高島 象一, 中村 亮介, 蓬田 昌伸, 磯野 拓也, 田島 健次, 梶 弘典, 佐藤 敏文, 第5回CSJ化学フェスタ, タワーホール船堀, 東京, Oct. 2015
Japanese

【157】大環状ポリ(3-ヘキシルチオフェン)の合成と特性評価　　　　　　　　　　　　　　　
蓬田 昌伸, 高島 象一, 磯野 拓也, 田島 健次, 梶 弘典, 佐藤 敏文, 第5回CSJ化学フェスタ, タワーホール船堀、東京, Oct. 2015
Japanese

【153】Synthesis of highly pure ethy end -flunctionalized poly(3-hexylthiophene)via Kumada catalyst transfer polycondensation　　　　　　　　　　　　　　　
Shoichi Takashima, Ryosuke Nakanura, Masanobu Yomogida, Takuya Isono, Kenji Tajima, Hironori Kaji, Toshifumi Satoh, Shoichi Takashima, Ryosuke Nakanura, Masanobu Yomogida, Takuya Isono, Kenji Tajima, Hironori Kaji, and Toshifumi Satoh, 03 Sep. 2015
English

【149】ポリ（３−ヘキシルチオフェン）（P３HT）の定量的末端エチニル化　　　　　　　　　　　　　　　
高島 象一, 中村 亮介, 蓬田 昌伸, 磯野 拓也, 田島 健次, 梶 弘典, 佐藤 敏文, ２０１５年度北海道高分子若手研究会, 定山渓グランドホテル(北海道）, 29 Aug. 2015
Japanese

酵素処理アオサ抽出デンプンを用いたバイオポリマー合成条件の検討
小林広弥, 上西将斗, 清野晃之, 小林孝紀, 藤田伸二, 田島健次, 高分子学会予稿集(CD-ROM), 64, 2, ROMBUNNO.1PC077, 25 Aug. 2015
Japanese

有機リン酸触媒を用いた環状エステルの無溶媒重合
齋藤達也, 相澤佑輔, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 2, ROMBUNNO.3E11, 25 Aug. 2015
Japanese

星型ポリ(N‐イソプロピルアクリルアミド)の熱応答特性―アーム数およびアーム長の影響―
吉田康平, TIAN Lin, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 2, ROMBUNNO.1PE035, 25 Aug. 2015
Japanese

【148】ポリ (3-ヘキシルチオフェン) (P3HT) の定量的末端エチニル化　　　　　　　　　　　　　　　
高島 象一, 中村 亮介, 蓬田 昌伸, 磯野 拓也, 田島 健次, 梶 弘典, 佐藤 敏文, 2015年度 北海道高分子若手研究会, 定山渓グランドホテル瑞苑, 札幌, Aug. 2015
Japanese

表面修飾によるナノフィブリル化バクテリアセルロースの機能化
大場淳矢, 田原功太郎, 磯野拓也, 甲野裕之, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 22nd, 128, 01 Jul. 2015
Japanese

Pichia pastorisを用いたセルロース合成酵素発現系の構築
砂川直輝, 鮫島正浩, 五十嵐圭日子, 田島健次, セルロース学会年次大会講演要旨集, 22nd, 62, 01 Jul. 2015
Japanese

Acetobacter xylinum由来セルロース合成酵素サブユニットDによる糖鎖基質認識
宇都卓也, 池田祐樹, 田島健次, YAO Min, 湯井敏文, セルロース学会年次大会講演要旨集, 22nd, 26, 27, 01 Jul. 2015
Japanese

ラセミラクチドのエナンチオマー選択的バルク重合
相澤佑輔, 齋藤達也, 磯野拓也, 田島健次, 寺田眞浩, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.2PD008, 12 May 2015
Japanese

表面修飾によるナノフィブリル化バクテリアセルロースの機能化
大場淳矢, 田原功太郎, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.2PC111, 12 May 2015
Japanese

分子内メタセシス反応によるサイズ制御された単分子ナノパーティクルの合成
渡部航大, 高田健司, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.1PB014, 12 May 2015
Japanese

ポリアセチレンを主鎖とするグラフトポリ(3‐ヘキシルチオフェン)の合成
高島象一, 蓬田昌伸, 中村亮介, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.1PH016, 12 May 2015
Japanese

リビング開環重合と分子内クリック環化反応を用いた三つ葉型ポリブチレンオキシドの精密合成
松野拓彦, 佐藤悠介, 磯野拓也, 田島健次, 覺知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.1PC013, 12 May 2015
Japanese

メタセシス触媒を用いた両末端ノルボルネン官能基化ポリラクチドの環化重合
笹森哲弥, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.1PF018, 12 May 2015
Japanese

分子内薗頭カップリングによる大環状ポリ(3‐ヘキシルチオフェン)の合成
蓬田昌伸, 中村亮介, 磯野拓也, 田島健次, 梶弘典, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.1PF014, 12 May 2015
Japanese

有機リン酸触媒を用いた環状エステルのバルク重合
齋藤達也, 相澤佑輔, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 64, 1, ROMBUNNO.2PC007, 12 May 2015
Japanese

Molecular dynamics simulation study of the AxCeSD octamer compelxed with cellulose chains
Toshifumi Yui, Takuya Uto, Yuki Ikeda, Kenji Tajima, Min Yao, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015, [Peer-reviewed]
AMER CHEMICAL SOC, English, Summary international conference

サイズ制御された分子内架橋ポリマーの合成
渡部航大, 高田健司, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 49th, 16, 2015
Japanese

ロジウム触媒を用いた末端エチニル化ポリチオフェンのグラフト重合
高島象一, 中村亮介, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 49th, 56, 2015
Japanese

ルテニウム触媒を用いた両末端ノルボルネン官能基化ポリ乳酸の環化重合
笹森哲弥, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 49th, 17, 2015
Japanese

バクテリアセルロース合成細菌由来セルラーゼCeSZの構造・機能解析
藤島あゆみ, 藤原孝彰, 加藤公児, 田島健次, 姚閔, 物構研サイエンスフェスタ要旨集, 3rd, 77, 2015
Japanese

分子内薗頭反応による大環状ポリ(3‐ヘキシルチオフェン)の合成
蓬田昌伸, 中村亮介, 磯野拓也, 田島健次, 梶弘典, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 49th, 53, 2015
Japanese

光分解性ブロック共重合体を用いた生分解性ナノ構造体の創製
齋藤憲吾, 孫翰笙, 磯野拓也, 田島健次, 陳文章, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 49th, 54, 2015
Japanese

酢酸菌セルロース生合成におけるアスコルビン酸添加効果
高橋佳奈, 穴井ひかる, 甲野裕之, 田島健次, 清水祐一, 高分子学会北海道支部研究発表会講演要旨集, 49th, 49, 2015
Japanese

クリック反応によるナノフィブリル化バクテリアセルロースの表面修飾
大場淳矢, 田原功太郎, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 49th, 52, 2015
Japanese

有機リン酸触媒を用いた環状エステルの塊状重合
齋藤達也, 相澤佑輔, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 49th, 55, 2015
Japanese

ナノフィブリル化バクテリアセルロースの形態観察と表面修飾による機能化
田原功太郎, 小瀬亮太, 大場淳矢, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 49th, 15, 2015
Japanese

Pichia pastorisを用いたセルロース合成酵素発現系の構築
砂川直輝, 鮫島正浩, 五十嵐圭日子, 田島健次, 日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 65th, ROMBUNNO.K18-07-1615, 2015
Japanese

Precise synthesis of cyclic-and star-shaped poly(3-hexylthiophene)s　　　　　　　　　　　　　　　
Ryosuke Nakamura, Daich Suemasa, Takuya Isono, Kenji Tajima, Hironori kaji, Toshifumi Satoh, The Ist Innovation Forum of Engineering Education(SPACC), 31 Oct. 2014
English

4級アンモニウム塩を用いたナノフィブリル化バクテリアセルロースの機能化
田原功太郎, 小瀬亮太, 大場淳矢, 磯野拓也, 佐藤敏文, 田島健次, 高分子学会予稿集(CD-ROM), 63, 2, ROMBUNNO.1PB100, 03 Sep. 2014
Japanese

【141】触媒移動型連鎖重縮合による末端エチニル化ポリチオフェンの精密合成　　　　　　　　　　　　　　　
蓬田 昌伸, 末政 大地, 中村 亮介, 磯野 拓也, 田島 健次, 梶 弘典, 佐藤 敏文, 2014年度北海道高分子若手研究会, 定山渓ビューホテル(北海道）, 28 Aug. 2014
Japanese

海藻由来新奇セルロース合成菌の解析
田原功太郎, 砂川直輝, 渡辺耕平, HAN Xuerong, 小瀬亮太, 佐藤敏文, 田島健次, セルロース学会年次大会講演要旨集, 21st, 135, 01 Jul. 2014
Japanese

メタノール資化性酵母Pichia pastorisを用いたセルロース合成酵素発現系の構築
砂川直輝, 田島健次, 鮫島正浩, 五十嵐圭日子, セルロース学会年次大会講演要旨集, 21st, 3, 4, 01 Jul. 2014
Japanese

酢酸菌セルロース合成酵素サブユニットDにおける糖鎖のダイナミクス挙動
池田祐樹, 宇都卓也, 田島健次, YAO Min, 湯井敏文, セルロース学会年次大会講演要旨集, 21st, 113, 01 Jul. 2014
Japanese

星型ポリ(N‐イソプロピルアクリルアミド)の合成と熱応答特性
吉田康平, ZHANG Yao, 磯野拓也, 田島健次, 覚知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.2C05, 09 May 2014
Japanese

リビング開環重合とクリック反応を用いた多環状ポリカプロラクトンの精密合成
松野拓彦, 佐藤悠介, 磯野拓也, 田島健次, 覺知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.2C04, 09 May 2014
Japanese

両親媒性タッドポール型ブロックコポリエーテルの合成
佐藤悠介, 宮地香奈, 磯野拓也, 田島健次, 覺知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.2C06, 09 May 2014
Japanese

ビス(4‐ニトロフェニル)リン酸を触媒に用いたβ‐ブチロラクトンの開環重合
齋藤達也, 牧口孝祐, 田島健次, 覚知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.1E27, 09 May 2014
Japanese

フォスファゼン塩基を触媒に用いたグリシジルアミン誘導体のリビング開環重合
浅井俊介, 佐藤悠介, 磯野拓也, 田島健次, 覚知豊次, 佐藤敏文, 高分子学会予稿集(CD-ROM), 63, 1, ROMBUNNO.1E28, 09 May 2014
Japanese

酢酸菌によるセルロース合成と発酵ナノセルロース(NFBC)の大量生産
田島健次, 小瀬亮太, 櫻井博章, 砂糖類・でん粉情報, 19, 44, 47, 10 Apr. 2014
農畜産業振興機構調査情報部, Japanese

酢酸菌によるセルロース合成と発酵ナノセルロース(NFBC)の大量生産
田島健次, 小瀬亮太, 櫻井博章, 機能材料, 34, 4, 57, 65, 04 Apr. 2014
シーエムシー出版, Japanese

Synthesis of cyclic and figure-eight-shaped block copolyether by combining t-Bu-P-4-catalyzed ring opening polymerization and click cyclization
Yusuke Satoh, Takuya Isono, Kenji Tajima, Toyoji Kakuchi, Toshifumi Satoh, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 247, Mar. 2014, [Peer-reviewed]
AMER CHEMICAL SOC, English, Summary international conference

Synthesis of maltose centered star-shaped polystyrenes via atom transfer radical polymerization and click reaction
Yao Zhang, Yohei Kondo, Daichi Suemasa, Takuya Isono, Kenji Tajima, Toyoji Kakuchi, Toshifumi Satoh, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 247, Mar. 2014, [Peer-reviewed]
AMER CHEMICAL SOC, English, Summary international conference

有機リン酸エステルを触媒に用いたβ‐ブチロラクトンのカチオン開環重合
齋藤達也, 牧口孝祐, 田島健次, 覚知豊次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2014, ROMBUNNO.2A04, 28 Jan. 2014
Japanese

分岐構造を有するポリ乳酸の合成とステレオコンプレックス形成能
近藤洋平, 磯野拓也, 田島健次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2014, ROMBUNNO.2A05, 28 Jan. 2014
Japanese

マルチクリック環化による8の字型ブロックコポリエーテルの精密合成
佐藤悠介, 宮地香奈, 磯野拓也, 田島健次, 覚知豊次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2014, ROMBUNNO.2A07, 28 Jan. 2014
Japanese

大環状ポリ(3‐ヘキシルチオフェン)の精密合成
末政大地, 齋藤憲吾, 中村亮介, 田島健次, 梶典弘, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2014, ROMBUNNO.2A08, 28 Jan. 2014
Japanese

リビング開環重合によるポリグリシジルアミン誘導体の精密合成
浅井俊介, 佐藤悠介, 磯野拓也, 田島健次, 覚知豊次, 佐藤敏文, 化学系学協会北海道支部冬季研究発表会講演要旨集(CD-ROM), 2014, ROMBUNNO.2A06, 28 Jan. 2014
Japanese

酢酸菌セルロース合成酵素サブユニットDによるセルロース分子鎖認識
宇都卓也, 池田祐樹, 田島健次, YAO Min, 湯井敏文, 繊維学会予稿集, 69, 1 (CD-ROM), ROMBUNNO.1H03, 2014
Japanese

エチニル基を有するセルロース誘導体のin vivo合成
渡辺耕平, 齋藤憲吾, 佐藤敏文, 田島健次, 高分子学会北海道支部研究発表会講演要旨集, 48th, 31, 2014
Japanese

リビング開環重合とクリック反応を用いた8の字型ポリエステルの精密合成
松野拓彦, 佐藤悠介, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 48th, 15, 2014
Japanese

星型ポリ(N‐イソプロピルアクリルアミド)の合成と水溶液中での熱応答特性
吉田康平, 張瑶, 磯野拓也, 田島健次, 佐藤敏文, 高分子学会北海道支部研究発表会講演要旨集, 48th, 22, 2014
Japanese

酢酸菌による発酵ナノセルロース(NFBC)の大量生産について
櫻井博章, 菊地裕人, 内野浩克, 田島健次, 精糖技術研究会講演要旨, 112th, 22, 26, 2014
Japanese

Production of nanofibrillated bacterial cellulose (NFBC) from waste glycerol using a bacterium
小瀬亮太, 田島健次, Cellulose Communications, 20, 4, 174, 177, Dec. 2013, [Invited]
Japanese, Introduction scientific journal

Functions of CcpAx and BcsD related to the cellulose synthesis in Gluconacetobacter
砂川直輝, 田島健次, Cellulose Communications, 20, 3, 134, 139, Sep. 2013, [Invited]
セルロース学会, Japanese, Introduction scientific journal

【128】末端エチニル化ポリ（3-ヘキシルチオフェン）の精密合成　　　　　　　　　　　　　　　
末政大地, 斎藤憲吾, 中村亮介, 田島健次, 斎藤敏文, 2013年度北海道高分子若手研究会, 札幌市アイヌ文化交流ｾﾝﾀｰ(北海道）, 31 Aug. 2013
Japanese

通気撹拌培養による廃グリセリンを原料としたナノフィブリル化バクテリアセルロース(NFBC)の大量生産
小瀬亮太, 田島健次, セルロース学会年次大会講演要旨集, 20th, 129, 01 Jul. 2013
Japanese

酢酸菌セルロース合成酵素サブユニットD(AxCeSD)に対する糖鎖ドッキング解析
池田祐樹, 城彗, 椎葉大偉, 田島健次, YAO Min, 湯井敏文, セルロース学会年次大会講演要旨集, 20th, 149, 01 Jul. 2013
Japanese

酢酸菌セルロース合成酵素サブユニットD(AxCeSD)に対する糖鎖ドッキング解析
池田祐樹, 城彗, 椎葉大偉, 田島健次, YAO Min, 湯井敏文, 繊維学会予稿集, 68, 1 (CD-ROM), ROMBUNNO.1PB41, 12 Jun. 2013
Japanese

Flavobacterium sp.UMI‐01のアルギン酸リアーゼとその遺伝子クラスター
井上晶, 高殿晃平, 池田祐輔, 田島健次, 尾島孝男, マリンバイオテクノロジー学会大会講演要旨集, 15th, 64, 01 Jun. 2013
Japanese

Flavobacterium sp.UMI‐01株の粗酵素を用いたアルギン酸からのα‐ケト酸の作出
村田安興, 井上晶, 田島健次, 尾島孝男, マリンバイオテクノロジー学会大会講演要旨集, 15th, 63, 01 Jun. 2013
Japanese

廃グリセリンからのナノフィブリル化バクテリアセルロースの(NFBC)の大量合成
小瀬亮太, 田島健次, 高分子学会予稿集(CD-ROM), 62, 1, ROMBUNNO.1PH100, 14 May 2013
Japanese

デンプン資化性菌Massilia sp.を用いたPHAの合成条件の検討
磯村尚之, 古賀実咲季, 清野晃之, 小林孝紀, 藤田伸二, 田島健次, 高分子学会予稿集(CD-ROM), 62, 1, ROMBUNNO.1PF088, 14 May 2013
Japanese

廃グリセリンからセルロースを合成可能な新奇バクテリアの単離
小瀬亮太, 吉田誠, 田島健次, 砂川直輝, 高分子学会予稿集(CD-ROM), 62, 1, ROMBUNNO.1PG099, 14 May 2013
Japanese

Flavobacterium sp.UMI‐01株由来アルギン酸リアーゼとその遺伝子クラスター
高殿晃平, 池田祐輔, 村田安興, RAHMAN M. M, 田島健次, 井上晶, 尾島孝男, 日本水産学会大会講演要旨集, 2013, 102, 26 Mar. 2013
Japanese

異なる地点の水温および気温の変動を用いた水温の変動の推定について
藤田伸二, 松村一弘, 小林孝紀, 中崎清彦, 須田孝徳, 田島健次, 曽根謙一, 日本水産工学会学術講演会講演論文集, 2013, 161, 164, 2013
Japanese

Synthesis of Cellulose by Bacteria
TAJIMA Kenji, NIPPON GOMU KYOKAISHI, 85, 12, 406, 411, Dec. 2012, [Invited]
Cellulose is the most abundant biopolymer in nature and is utilized in a wide variety of industries. Although plants produce the most cellulose, some animals and bacteria also produce cellulose. <i>Acetobacter</i> (=<i>Gluconacetobacter</i>), which is a Gram-negative bacterium, produces cellulose called bacterial cellulose (BC) from glucose on the surface of a culture medium. Even in plants, there is little knowledge concerning the mechanisms of cellulose biosynthesis. Due to its ease of handling, <i>Acetobacter xylinum</i> (<i>A. xylinum</i>) has been studied as a model organism of cellulose production. BC has exceptional physicochemical properties, such as ultrafine reticulated structure, high crystallinity, high tensile strength, high hydrophilicity, moldability during formation, and biocompatibility, although its chemical structure is the same as those of the cellulose produced by plants and algae. These remarkable characteristics are of interest for the development and manufacture of a wide range of materials, such as food matrices, dietary fiber, acoustic membranes, special biomaterials. In this article, I introduce cellulose synthesis by bacteria (features and applications) and the synthetic mechanism of BC., THE SOCIRETY OF RUBBER SCIENCE AND TECHNOLOGYY, JAPAN, Japanese

Flavobacterium sp.UMI‐01株由来の新奇アルギン酸リアーゼ
高殿晃平, 村田安興, RAHMAN M.M, 田島健次, 井上晶, 尾島孝男, 日本農芸化学会北海道支部講演会講演要旨, 2012, 41, 01 Nov. 2012
Japanese

PilZによるc‐di‐GMPの認識機構に関する構造学的研究
藤原孝彰, 薦田圭介, 桜井直文, 田島健次, 田中勳, 姚閔, 日本結晶学会年会講演要旨集, 2012, 99, 25 Oct. 2012
Japanese

熱安定性酵素を用いたポリヒドロキシアルカン酸のin vitro合成
橋本佳季, HAN Xuerong, 佐藤康治, 田島健次, 田口精一, 大利徹, 高分子学会予稿集(CD-ROM), 61, 2, ROMBUNNO.1PB118, 05 Sep. 2012
Japanese

海藻から単離したバクテリアを用いたポリヒドロキシアルカン酸の合成
HAN Xuerong, 栗城夢実, 佐藤康治, 須田孝徳, 田島健次, 小林孝紀, 藤田伸二, 高分子学会予稿集(CD-ROM), 61, 2, ROMBUNNO.1PA119, 05 Sep. 2012
Japanese

デンプン資化性菌Massilia sp.を用いたPHAの合成条件の検討
磯村尚之, 古賀実咲季, 清野晃之, 小林孝紀, 藤田伸二, 須田孝徳, 田島健次, 高分子学会予稿集(CD-ROM), 61, 2, ROMBUNNO.1PB120, 05 Sep. 2012
Japanese

バイオディーゼル燃料副生成物からのバクテリアセルロースの生産
小瀬亮太, 吉田誠, 砂川直輝, 田島健次, 大利徹, セルロース学会年次大会講演要旨集, 19th, 122, 01 Jul. 2012
Japanese

遺伝子組換え酢酸菌によるセルロース誘導体の合成
佐藤智章, 砂川直輝, 小瀬亮太, 田島健次, 大利徹, セルロース学会年次大会講演要旨集, 19th, 61, 01 Jul. 2012
Japanese

遺伝子組換え酢酸菌によるGlcNAc含有バクテリアセルロースの合成
砂川直輝, 今井友也, YAO Min, 小瀬亮太, 田島健次, 大利徹, セルロース学会年次大会講演要旨集, 19th, 15, 16, 01 Jul. 2012
Japanese

酢酸菌のセルロース合成におけるbglxAおよび下流遺伝子の機能
砂川直輝, 松田恵利子, 小瀬亮太, YAO Min, 田島健次, 大利徹, セルロース学会年次大会講演要旨集, 19th, 133, 01 Jul. 2012
Japanese

気温の変動を用いた水温の変動の推定について
藤田 伸二, 松村 一弘, 小林 孝紀, 中崎 清彦, 田島 健次, 曽根 謙一, 日本水産工学会学術講演会講演論文集, 24, 205, 208, 18 May 2012
Japanese

Flavobacterium sp.UMI‐01株由来エンド型アルギン酸リアーゼの性状解析
高殿晃平, 村田安興, 安部秀昭, RAHMAN M. M, 田島健次, 井上晶, 尾島孝男, 日本水産学会大会講演要旨集, 2012, 105, 26 Mar. 2012
Japanese

気温の変動を用いた水温の変動の推定について
藤田伸二, 松村一弘, 小林孝紀, 中崎清彦, 田島健次, 曽根謙一, 日本水産工学会学術講演会講演論文集, 2012, 205, 208, 2012
Japanese

Development of polyhydroxyalkanoate (PHA)‐based functional polymeric materials
田島健次, Cellulose Communications, 18, 4, 170, 175, Dec. 2011, [Invited]
セルロース学会, Japanese, Introduction scientific journal

Pseudomonas sp.SG4502由来耐熱性PHA合成酵素と乳酸重合酵素としての利用
田島健次, HAN Xuerong, 佐藤康治, 石井綾子, 荒木祐治, 棟方正信, 田口精一, 高分子学会予稿集(CD-ROM), 60, 2 Disk1, ROMBUNNO.1PC117, 13 Sep. 2011
Japanese

酢酸菌への(1→3)‐β‐グルカン繊維分泌能付与の試み
中川理, 玉元遊, 河野信, 田島健次, 横田慎吾, 近藤哲男, セルロース学会年次大会講演要旨集, 18th, 129, 01 Jul. 2011
Japanese

Enterobacter sp.CJF002株におけるバクテリアセルロース合成の解析
砂川直輝, 細田真梨子, 田島健次, 河野信, YAO Min, 佐藤康治, 大利徹, セルロース学会年次大会講演要旨集, 18th, 137, 01 Jul. 2011
Japanese

NMR測定のためのバクテリアセルロースの任意位置への¹³Cラベル化への試み
惠良田知樹, 大東泰三, 田島健次, セルロース学会年次大会講演要旨集, 18th, 32, 33, 01 Jul. 2011
Japanese

酢酸菌セルロース合成酵素CeSDのN末端部位における挙動と基質認識
中原慶介, 富永宏二, 田島健次, YAO Min, 湯井敏文, セルロース学会年次大会講演要旨集, 18th, 130, 01 Jul. 2011
Japanese

耐熱性PHA合成細菌由来PHA合成酵素遺伝子のクローニングとその解析
佐藤康治, 松谷枝里子, 韓雪容, 中本翔, 田島健次, 松島得雄, 棟方正信, 日本農芸化学会大会講演要旨集, 2011, 196, 05 Mar. 2011
Japanese

海藻由来ポリヒドロキシアルカン酸合成菌の単離・同定
栗城夢実, 田島健次, 佐藤康治, 尾島孝男, 小林孝紀, 大利徹, 高分子学会北海道支部研究発表会講演要旨集, 45th, 47, 01 Feb. 2011
Japanese

皮膚における脂肪細胞の効果―皮膚を構成する他細胞との相互作用
大木菜津美, 金山寿之, 佐藤康治, 田島健次, 大利徹, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 45th, 46, 01 Feb. 2011
Japanese

官能基修飾によるポリヒドロキシアルカン酸の機能化
岩本浩介, 谷尾亮, 佐藤康治, 田島健次, 堺井亮介, 佐藤敏文, 鳥谷部哲也, 松島得雄, 覚知豊次, 大利徹, 棟方正信, 高分子学会予稿集(CD-ROM), 59, 2 Disk1, ROMBUNNO.1PD114, 01 Sep. 2010
Japanese

改良水‐有機溶媒二相反応系による新規ポリヒドロキシアルカン酸の合成
HAN Xuerong, 佐藤康治, 田島健次, 鳥谷部哲也, 松島得雄, 大利徹, 棟方正信, 高分子学会予稿集(CD-ROM), 59, 2 Disk1, ROMBUNNO.1PC113, 01 Sep. 2010
Japanese

Acetobacter xylinumにおけるbglxA下流遺伝子のクローニングと解析
松田恵利子, 砂川直輝, 吉田誠, 田島健次, 佐藤康治, 棟方正信, 大利徹, セルロース学会年次大会講演要旨集, 17th, 104, 01 Jul. 2010
Japanese

酢酸菌セルロース合成酵素CeSDと基質セロペンタオース間の相互作用解析
富永宏二, 田島健次, YAO Min, 湯井敏文, セルロース学会年次大会講演要旨集, 17th, 105, 01 Jul. 2010
Japanese

酢酸菌セルロース合成におけるAxCesD N末端の機能解析
砂川直輝, 藤原孝彰, 田島健次, YAO Min, 佐藤康治, 棟方正信, 田中勲, 大利徹, セルロース学会年次大会講演要旨集, 17th, 110, 01 Jul. 2010
Japanese

酢酸菌由来CesDとCcpAxの相互作用解析
藤原孝彰, 尾瀬農之, 姚閔, 田島健次, 田中勲, 日本蛋白質科学会年会プログラム・要旨集, 10th, 144, 15 May 2010
Japanese

酢酸菌由来AxCeSDとセロペンタオース複合体の分子動力学研究
冨永宏二, 田島健次, 姚閔, 林幸男, 湯井敏文, 日本農芸化学会大会講演要旨集, 2010, 270, 05 Mar. 2010
Japanese

Laboratory of Tissue Regeneration Engineering, Division of Biotechnology and Macro-molecular Chemistry, Graduate School of Engineering, Hokkaido University
田島 健次, JAPAN TAPPI JOURNAL, 64, 2, 156, 157, Feb. 2010, [Invited]
JAPAN TECHNICAL ASSOCIATION OF THE PULP AND PAPER INDUSTRY, Japanese

バイオディーゼル副生成物を原料としたバクテリアセルロース合成
砂川直輝, 吉田誠, 松田恵利子, 細田真梨子, 佐藤康冶, 松島得雄, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 44th, 38, 26 Jan. 2010
Japanese

中鎖長ポリヒドロキシアルカン酸の再生医療用マテリアルへの応用
青木陽平, 岩本浩介, 佐藤康治, 松島得雄, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 44th, 39, 26 Jan. 2010
Japanese

バイオディーゼル副生成物を原料としたバクテリアセルロース合成
砂川直輝, 吉田誠, 松田恵利子, 細田真梨子, 佐藤康治, 松島得雄, 田島健次, 棟方正信, 大利徹, 日本生物工学会シンポジウム講演要旨集, 2010, 33, 2010
Japanese

Fusion of Paper and Electronic Media ―Paper Based Electronic Paper―
田島健次, 日本画像学会誌, 49, 4, 276, 281, 2010, [Invited]
Japanese, Introduction scientific journal

バクテリアセルロース(BC)を用いて新規表示デバイス　　　　　　　　　　　　　　　
田島 健次, マテリアルステージ, 10, 2, 1, 2, 2010, [Invited]
Japanese, Introduction commerce magazine

Development of a new display device using cellulose produced by bacteria
田島 健次, Bio industry, 27, 1, 65, 70, Jan. 2010, [Invited]
シーエムシー出版, Japanese, Introduction commerce magazine

バイオディーゼル廃グリセロールの有効利活用技術 微生物を活用したバイオポリマーへの物質変換
佐藤康治, 田島健次, 松島得雄, 大嶋武, 棟方正信, 環境技術, 38, 11, 774, 778, Nov. 2009, [Invited]
環境技術学会, Japanese, Introduction scientific journal

FeO_X複合触媒による発酵残渣からの有用化学物質の製造
舟井啓, 佐藤由実, 佐藤康治, 田島健次, 多湖輝興, 増田隆夫, 触媒討論会討論会A予稿集, 104th, 138, 27 Sep. 2009
Japanese

乳酸重合酵素によるP(LA‐co‐3HB)の化学酵素合成
田島健次, 佐藤康治, 佐藤敏文, 伊藤瑠美, HAN Xuerong, 田口精一, 覚知豊次, 棟方正信, 高分子学会予稿集(CD-ROM), 58, 2 Disk1, ROMBUNNO.3PC149, 01 Sep. 2009
Japanese

分子動力学シミュレーション計算による溶媒和セルロース結晶材料のダイナミックス
湯井敏文, 冨永宏二, 椎葉大偉, 宮田竜彦, 平田文男, 田島健次, MIN Yao, 高分子学会予稿集(CD-ROM), 58, 2 Disk1, ROMBUNNO.1R-03, 01 Sep. 2009
Japanese

セルロース合成における酢酸菌とエンテロバクターの比較
細田真梨子, 砂川直輝, 吉田誠, 田島健次, 佐藤康治, 棟方正信, セルロース学会年次大会講演要旨集, 16th, 83, 20 Jun. 2009
Japanese

酢酸菌由来セルロース合成関連タンパク質の調製
藤原孝彰, 尾瀬農之, 田島健次, 田中勲, YAO Min, セルロース学会年次大会講演要旨集, 16th, 151, 152, 20 Jun. 2009
Japanese

酢酸菌への(1→3)‐β‐グルカン合成能の導入
玉元遊, 富田陽子, 河野信, 田島健次, 棟方正信, 近藤哲男, セルロース学会年次大会講演要旨集, 16th, 85, 20 Jun. 2009
Japanese

酢酸菌のセルロース生合成におけるAxCesCの機能解析
吉田誠, 砂川直輝, 田島健次, 佐藤康治, 棟方正信, セルロース学会年次大会講演要旨集, 16th, 84, 20 Jun. 2009
Japanese

酢酸菌セルロース合成におけるターミナルコンプレックスの機能解析
砂川直輝, HU SongQing, GAO YongGui, 田島健次, YAO Min, 依田貴範, 志村大輔, 河野信, 佐藤康治, 田中勲, 棟方正信, セルロース学会年次大会講演要旨集, 16th, 82, 20 Jun. 2009
Japanese

バクテリアセルロースを用いた新規デバイスの開発
田島健次, 砂川直輝, 佐藤康治, 棟方正信, 平野成伸, 近藤均, 松島得雄, 石澤秀太, セルロース学会年次大会講演要旨集, 16th, 35, 36, 20 Jun. 2009
Japanese

乳酸重合酵素によるP(LA‐co‐3HB)の化学酵素合成
田島健次, 佐藤康治, 佐藤敏文, 伊藤瑠美, HAN Xuerong, 田口精一, 覚知豊次, 棟方正信, 高分子学会予稿集(CD-ROM), 58, 1 Disk1, 3PA151, 12 May 2009
Japanese

酢酸菌セルロース合成におけるAxCesDの機能解析
砂川直輝, HU SongQing, GAO YongGui, 田島健次, MIN Yao, 依田貴範, 志村大輔, 河野信, 佐藤康治, 田中勲, 棟方正信, 日本農芸化学会大会講演要旨集, 2009, 319, 05 Mar. 2009
Japanese

新規PHA合成細菌からのPHA合成関連遺伝子のクローニングとその解析
佐藤康治, 松谷枝里子, 韓雪容, 田島健次, 松島得雄, 棟方正信, 日本農芸化学会大会講演要旨集, 2009, 126, 05 Mar. 2009
Japanese

鮭皮由来コラーゲンを利用した薬剤毒性評価用ヒト人工皮膚モデルの作製
大谷奈緒, 永井展裕, 金山寿之, 佐藤康治, 田島健次, 棟方正信, 再生医療, 8, 230, 05 Feb. 2009
Japanese

新規中鎖長PHA合成細菌からのPHA生合成関連遺伝子のクローニングとその解析
松谷枝里子, 佐藤康治, 韓雪容, 中本翔, 田島健次, 松島得雄, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 63, 03 Feb. 2009
Japanese

酢酸菌セルロース合成におけるAxCesDの機能解析
砂川直輝, HU SongQing, GAO YongGui, 田島健次, YAO Min, 依田貴範, 志村大輔, 河野信, 佐藤康治, 田中勲, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 60, 03 Feb. 2009
Japanese

新規中鎖長Polyhydroxyalkanoate合成細菌の単離および同定
中本翔, 佐藤康治, 田島健次, 松島得雄, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 62, 03 Feb. 2009
Japanese

鮭皮コラーゲンを用いた関節軟骨の再生医療
南沢樹梨, 永井展裕, 佐藤康治, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 43rd, 61, 03 Feb. 2009
Japanese

Cellulase is necessary for cellulose production?
田島健次, Cellulose Communications, 15, 4, 158, 163, Nov. 2008, [Invited]
セルロ-ス学会, Japanese, Introduction scientific journal

中温にてPHA合成能を有する新規細菌からのPHA生合成関連遺伝子のクローニング
松谷枝里子, HAN Xuerong, 中本翔, 佐藤康治, 田島健次, 松島得雄, 棟方正信, 高分子学会予稿集(CD-ROM), 57, 2 Disk1, 3PA119, 09 Sep. 2008
Japanese

バイオディーゼル燃料副生成物を原料としたポリヒドロキシアルカン酸合成
佐藤康治, 中本翔, 田島健次, 松島得雄, 棟方正信, 高分子学会予稿集(CD-ROM), 57, 2 Disk1, 3PB122, 09 Sep. 2008
Japanese

農産資源を原料としたポリヒドロキシアルカン酸合成
中本翔, 佐藤康治, 田島健次, 松島得雄, 棟方正信, 高分子学会予稿集(CD-ROM), 57, 2 Disk1, 3PA123, 09 Sep. 2008
Japanese

新規水‐有機溶媒二相反応系によるP(3HB)の合成
HAN Xuerong, 田島健次, 佐藤康治, 松島得雄, 棟方正信, 高分子学会予稿集(CD-ROM), 57, 2 Disk1, 3PB120, 09 Sep. 2008
Japanese

Bacillus sp.INT005由来polyhydroxyalkanoate合成酵素遺伝子組換え体を用いたpolyhydroxyalkanoateの生合成
松谷枝里子, 佐藤康治, 田島健次, 松島得雄, 棟方正信, 日本生物工学会大会講演要旨集, 60th, 205, 11 Jul. 2008
Japanese

新規中鎖長PHA合成細菌の単離及び同定
中本翔, 佐藤康治, 田島健次, 松島得雄, 棟方正信, 日本生物工学会大会講演要旨集, 60th, 205, 11 Jul. 2008
Japanese

ヒト歯周靭帯線維芽細胞の走化性因子に対する遊走能測定方法の比較
佐藤康治, 高橋麻貴子, 田島健次, 永井展裕, 棟方正信, 再生医療, 7, 268, 22 Feb. 2008
Japanese

鮭皮コラーゲンscaffoldを利用したin vitro人工歯根膜の作製
平川歩, 永井展裕, 森一生, 佐藤康治, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 36, 29 Jan. 2008
Japanese

鮭皮コラーゲンを利用した細胞シート重層化による人工皮膚モデルの開発
高橋さやか, 永井展裕, 金山寿之, 大谷奈緒, 森一生, 佐藤康治, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 38, 29 Jan. 2008
Japanese

鮭皮コラーゲン由来伸縮性ゲルの伸展培養基材への応用
金山寿之, 永井展裕, 森一生, 佐藤康治, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 35, 29 Jan. 2008
Japanese

ヒト歯周靭帯線維芽細胞の走化性因子に対する遊走能測定方法の比較
高橋麻貴子, 佐藤康治, 永井展裕, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 33, 29 Jan. 2008
Japanese

ビタミンC含有コラーゲンマイクロ粒子の開発
伊藤理沙, 永井展裕, 柚木俊二, 森一生, 佐藤康治, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 37, 29 Jan. 2008
Japanese

Bacillus sp.INT005由来polyhydroxyalkanoate合成酵素遺伝子組換え体を用いたpolyhydroxyalkanoateの生合成
松谷枝里子, 佐藤康治, 田島健次, 棟方正信, 高分子学会北海道支部研究発表会講演要旨集, 42nd, 34, Jan. 2008
Japanese

1Ip02 Isolation and characterization of a novel bacterium accumulating medium-chain-length polyhydroxyalkanoate
NAKAMOTO Sho, SATOH Yasuharu, TAJIMA Kenji, MATSUSHIMA Tokuo, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 20, 205, 205, 2008
日本生物工学会, Japanese

1Ip01 Biosynthesis of polyhydroxyalkanoate by recombinant bacteria expressing the PHA synthase genes phaRC from Bacillus sp. INT005
MATSUTANI Eriko, SATOH Yasuharu, TAJIMA Kenji, MATSUSHIMA Tokuo, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 20, 205, 205, 2008
日本生物工学会, Japanese

MFI型ゼオライトによる水相中希薄チオフェノール選択除去
大堀武蔵, 田島健次, 佐藤康治, 多湖輝興, 増田隆夫, ゼオライト研究発表会講演予稿集, 23rd, 86, 07 Nov. 2007
Japanese

伸縮性サケ皮コラーゲンゲルを利用した細胞培養
金山寿之, 永井展裕, 森一生, 柚木俊二, 佐藤康治, 田島健次, 棟方正信, 日本生物工学会大会講演要旨集, 59th, 139, 02 Aug. 2007
Japanese

2F11-2 The use of elastic salmon collagen gel for cell culture
KANAYAMA Toshiyuki, NAGAI Nobuhiro, MORI Kazuo, YUNOKI Shunji, SATOH Yasuharu, TAJIMA Kenji, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 19, 139, 139, 2007
日本生物工学会, Japanese

微生物を用いない高機能性PHA新規合成法の開発
佐藤康治, 田島健次, 棟方正信, ゼネラル石油研究奨励財団研究報告書, 12, 8, 12, 01 Dec. 2006
Japanese

海洋性コラーゲンを用いたバイオマテリアルの開発
永井展裕, 柚木俊二, 森一生, 金山寿之, 佐藤康治, 田島健次, 棟方正信, 高分子学会予稿集(CD-ROM), 55, 2 Disk1, 3PA119, 05 Sep. 2006
Japanese

Ni‐Y型ゼオライトによる水相中希薄チオフェノール高効率除去
多湖輝興, 齋藤聡敏, 大堀武蔵, 田島健次, 田口精一, 佐藤敏文, 佐藤康治, 増田隆夫, 化学工学会秋季大会研究発表講演要旨集(CD-ROM), 38th, Q208, 759, 16 Aug. 2006
公益社団法人 化学工学会, Japanese

ナノ線維構造を有する組織工学用伸縮性コラーゲン
金山寿之, 永井展裕, 森一生, 柚木俊二, 佐藤康治, 田島健次, 棟方正信, 日本生物工学会大会講演要旨集, 58th, 201, 03 Aug. 2006
Japanese

ウシ歯セメント質由来細胞走化因子の同定
西尾恵, 佐藤康治, 田島健次, 棟方正信, 日本生物工学会大会講演要旨集, 58th, 205, 03 Aug. 2006
Japanese

酢酸菌セルロース合成酵素オペロン上流に存在する遺伝子の機能―その2―
依田貴範, 田島健次, 酒井麻衣子, 佐藤康治, 棟方正信, セルロース学会年次大会講演要旨集, 13th, 90, 01 Jul. 2006
Japanese

水‐有機溶媒二相反応系によるP(3HB)の化学酵素合成
石井綾子, 中澤浩二, 田島健次, 佐藤康治, 佐藤敏文, 覚知豊次, 棟方正信, 高分子学会予稿集(CD-ROM), 55, 1 Disk1, 1PF178, 10 May 2006
Japanese

KEGG as a glycome informatics resource
Yoshiaki Yasutake, Shin Kawano, Kenji Tajima, Min Yao, Yasuharu Satoh, Masanobu Munekata, Isao Tanaka, Glycobiology, 16, 5, 63R, 70R, 2006
Oxford University Press (OUP)

The forefront of the bioplastics synthesis research‐the crossover of invivo and invitro‐
田口精一, 田島健次, 佐藤康治, 高分子加工, 55, 1, 30, 43, Jan. 2006, [Invited]
高分子刊行会, Japanese

3K11-1 Identification of chemotactic factors derived from bovine cementum
NISHIO Megumi, SATO Yasuharu, TAJIMA Kenji, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 18, 205, 205, 2006
日本生物工学会, Japanese

2K12-1 Preparation of an in vitro artificial periodontal ligament tissue using fish collagen scaffold
NAGAI Nobuhiro, HIRAKAWA Ayumi, MORI Kazuo, YUNOKI Shunji, SATOH Yasuharu, TAJIMA Kenji, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 18, 202, 202, 2006
日本生物工学会, Japanese

2K11-5 Elastic collagen with nanofibrous structure for tissue engineering
KANAYAMA Toshiyuki, NAGAI Nobuhiro, MORI Kazuo, YUNOKI Shunji, SATOH Yasuharu, TAJIMA Kenji, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 18, 201, 201, 2006
日本生物工学会, Japanese

細胞走化活性試験の条件検討とウシ歯セメント質由来細胞走化因子の同定
衛藤周平, 西尾恵, 佐藤康治, 田島健次, 棟方正信, 日本生物工学会大会講演要旨集, 2005, 217, 25 Sep. 2005
Japanese

酢酸菌セルロース合成酵素オペロン上流に存在する遺伝子の機能
依田貴範, 田島健次, 山下ひとみ, 佐藤康治, 棟方正信, 河野信, 安武義晃, YAO Min, 田中勲, セルロース学会年次大会講演要旨集, 12th, 98, 11 Jul. 2005
Japanese

酢酸菌のセルロース合成におけるorf2の機能
依田貴範, 田島健次, 棟方正信, 木村聡, 近藤哲男, 森田光博, 日本農芸化学会大会講演要旨集, 2005, 325, 05 Mar. 2005
Japanese

進化工学的手法により改変されたPseudomonas sp.61‐3由来Polyhydroxyalkanoate(PHA)合成酵素(PhaC1)の精製と解析
佐藤康治, 勝田直介, 田島健次, 中沢浩二, 松本謙一郎, 高瀬和真, 田口精一, 土肥義治, 陳方正信, 日本農芸化学会大会講演要旨集, 2005, 237, 05 Mar. 2005
Japanese

1H17-1 Determination of chemotaxisis assay conditions and identification of chemotactic factors derived from bovine cementum
ETOH Shuhei, NISHIO Megumi, SATOH Yasuharu, TAJIMA Kenji, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 17, 217, 217, 2005
日本生物工学会, Japanese

Bacillus sp. INT005およびB. megaterium由来Polyhydroxyalkanoate合成酵素の比較
佐藤康治, 田島健次, 棟方正信, 高分子学会予稿集(CD-ROM), 53, 2 Disk1, 3PC141, 01 Sep. 2004
Japanese

Bacillus sp. INT005由来PHA合成酵素の解析とin vitro PHA合成への応用
中沢浩二, 佐藤康治, 田島健次, 勝田直介, 棟方正信, 高分子学会予稿集(CD-ROM), 53, 2 Disk1, 3PA179, 01 Sep. 2004
Japanese

人工進化型polyhydroxyalkanoate(PHA) synthaseのkinetic analysis
松本謙一郎, 佐藤康治, 田島健次, 棟方正信, 高瀬和真, 土肥義治, 田口精一, 高分子学会予稿集(CD-ROM), 53, 2 Disk1, 3PB178, 01 Sep. 2004
Japanese

Bacillus sp. INT005及びSynechocystis sp. PCC6803由来PHA合成酵素サブユニットの相互作用解析
勝田直介, 佐藤康治, 田島健次, 中沢浩二, 棟方正信, 高分子学会予稿集(CD-ROM), 53, 2 Disk1, 3PD142, 01 Sep. 2004
Japanese

魚類由来コラーゲン線維ゲルの作成と細胞培養への応用
永井展裕, 佐藤康治, 田島健次, 棟方正信, 柚木俊二, 高分子学会予稿集(CD-ROM), 53, 2 Disk1, 3PC115, 01 Sep. 2004
Japanese

魚類由来コラーゲン線維ゲルの作成と細胞培養への応用
永井展裕, 柚木俊二, 佐藤康治, 田島健次, 棟方正信, 日本生物工学会大会講演要旨集, 2004, 183, 25 Aug. 2004
Japanese

ウシ歯セメント質抽出物の歯周組織細胞への影響
高橋典敬, 佐藤康治, 田島健次, 棟方正信, 日本生物工学会大会講演要旨集, 2004, 192, 25 Aug. 2004
Japanese

酢酸菌のセルロース合成におけるBcsD遺伝子の機能
志村大輔, 河野信, 田島健次, 藤原政司, 恵良田知樹, 棟方正信, 高井光男, セルロース学会年次大会講演要旨集, 11th, 106, 01 Jul. 2004
Japanese

酢酸菌のセルロース合成におけるORF2遺伝子の機能解明
依田貴範, 田島健次, 棟方正信, 木村聡, 近藤哲男, 森田光博, セルロース学会年次大会講演要旨集, 11th, 107, 01 Jul. 2004
Japanese

2F11-1 Preparation and application to a cell culture of fish collagen fibrillar gel
NAGAI Nobuhiro, YUNOKI Shunji, SATOH Yasuharu, TAJIMA Kenji, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 16, 183, 183, 2004
日本生物工学会, Japanese

3F11-1 Effect of bovine cementum extracts on periodontal cells
TAKAHASHI Noritaka, SATOH Yasuharu, TAJIMA Kenji, MUNEKATA Masanobu, 日本生物工学会大会講演要旨集, 16, 192, 192, 2004
日本生物工学会, Japanese

重合酵素によるPHAのin vitro合成
田島健次, 佐藤康治, 恵良田知樹, 棟方正信, 蒲池元昭, 高分子学会予稿集, 52, 14, 4216, 4217, 10 Sep. 2003
Japanese

バイオポリマーの合成機構解明と機能性高分子材料への応用
田島健次, 日本生物工学会シンポジウム講演要旨集, 2003, 15, 21, 05 Aug. 2003
Japanese

有機溶媒重層によるPHAの効率的酵素合成
田島健次, 恵良田知樹, 佐藤康治, 棟方正信, 蒲池元昭, 高分子学会予稿集, 52, 5, 996, 08 May 2003
Japanese

マリンコラーゲンscaffoldの作成とその機能
坂田麻衣子, 永井展裕, 田島健次, 棟方正信, 日本農芸化学会北海道支部・北海道農芸化学協会シンポジウム及び合同学術講演会講演要旨, 2003, 22, 2003
Japanese

バイオポリマーの合成機構解明と機能性高分子材料への応用(北日本支部シンポジウム開催報告)
田島 健次, 生物工学会誌 : seibutsu-kogaku kaishi, 81, 12, 543, 543, 2003
日本生物工学会, Japanese

Characterization of PHA synthase from Bacillus sp. strain INT005
Nakazawa Koji, Satoh Yasuharu, Tajima Kenji, Katsuda Naosuke, Munekata Masanobu, 日本生物工学会大会講演要旨集, 15, 67, 67, 2003
日本生物工学会, Japanese

Comparison of PHA synthases from Bacillus sp. INT005 and Synechocystis sp. PCC6803 in vitro
Katsuda Naosuke, Satoh Yasuharu, Tajima Kenji, Nakazawa Koji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 15, 68, 68, 2003
日本生物工学会, Japanese

Culture of periodontal ligament cells in fish collagen scaffolds
Nagai Nobuhiro, Sakata Maiko, Satoh Yasuharu, Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 15, 92, 92, 2003
日本生物工学会, Japanese

遺伝子組換えによる高機能性セルロース誘導体のin vivo合成
田島健次, 高井光男, 棟方正信, 恵良田知樹, 柴肇一, ゼネラル石油研究奨励財団研究報告書, 10, 1, 7, 01 Dec. 2002
Japanese

Acetobacter xylinum由来内在性β‐blucosidaseのセルロース合成への関与
山下ひとみ, 田島健次, 中島克敏, 棟方正信, 高井光男, 日本生物工学会大会講演要旨集, 2002, 58, 25 Sep. 2002
Japanese

酢酸菌ATCC23769株とATCC53582株間でのセルロース生合成能の比較
河野信, 上森弓華子, 田島健次, 山下ひとみ, 藤原政司, 恵良田知樹, 棟方正信, 高井光男, セルロース学会年次大会講演要旨集, 9th, 68, 25 Jun. 2002
Japanese

セルロース産生酢酸菌培養におけるリグニンスルホン酸の添加効果
吉岡亜希, 藤原政司, 田島健次, 恵良田知樹, 高井光男, セルロース学会年次大会講演要旨集, 9th, 65, 25 Jun. 2002
Japanese

バイオポリマーを機能性高分子材料に!?
田島健次, 生物工学会誌, 80, 3, 125, 125, 25 Mar. 2002
日本生物工学会, Japanese

学会レポート ISBP2002に出席して
田島 健次, Cellulose communications, 9, 4, 233, 236, 2002, [Invited]
セルロ-ス学会, Japanese

431 Endogenous b-glucosidase from Acetobacter xylinum was involved in the cellulose synthesis
Yamashita Hitomi, Tajima Kenji, Nakajima Katsutoshi, Munekata Masanobu, Takai Mitsuo, 日本生物工学会大会講演要旨集, 14, 58, 58, 2002
日本生物工学会, Japanese

バクテリアセルロースによる生分解性材料の開発 (特集 環境バイオテクノロジーの事業化)
田島 健次, ケミカルエンジニヤリング, 46, 9, 694, 698, Sep. 2001, [Invited]
化学工業社, Japanese

酢酸菌セルロース生合成時のエンドグルカナーゼ作用部位
河野信, 上森弓華子, 中島克敏, 田島健次, 甲野裕之, 恵良田知樹, 高井光男, 高部圭司, 藤田稔, セルロース学会年次大会講演要旨集, 8th, 66, 02 Jul. 2001
Japanese

酢酸菌βグルコシダーゼのセルロース合成における働き
山下ひとみ, 田島健次, 中島克敏, 河野信, 甲野裕之, 柴肇一, 藤原政司, 棟方正信, 高井光男, セルロース学会年次大会講演要旨集, 8th, 67, 02 Jul. 2001
Japanese

Isolation and characterization of bacterium producing PHA from gas field
Tajima Kenji, Igari Takaaki, Nishimura Daisuke, Nakamura Maiko, Munekata Masanobu, 日本生物工学会大会講演要旨集, 13, 291, 291, 2001
日本生物工学会, Japanese

Cloning and ana1ysis of PHA biosynthesis genes in Bacillus sp. INTO05 strain.
Minamoto Norimasa, Satoh Yasuharu, Tajima Kenji, Shiba Toshikazu, Munekata Masanobu, 日本生物工学会大会講演要旨集, 13, 292, 292, 2001
日本生物工学会, Japanese

Analysis of in vitro PHB synthesis reaction from acetate.
Satoh Yasuharu, Tajima Kenji, Erata Tomoki, Shiba Toshikazu, Munekata Masanobu, 日本生物工学会大会講演要旨集, 13, 292, 292, 2001
日本生物工学会, Japanese

バクテリアセルロースによる生分解性材料の開発　　　　　　　　　　　　　　　
田島健次, ケミカルエンジニアリング, 46, 9, 694, 698, 2001, [Invited]

Poly(P)‐AMP phosphotransferaseを利用した新しいATP再生系の構築
亀田充史, 柴肇一, 佐藤康治, 田島健次, 棟方正信, 石毛和也, 野口利忠, 日本生物工学会大会講演要旨集, 2000, 222, 10 Jul. 2000
Japanese

酢酸菌のセルロース合成におけるエンドグルカナーゼの働き
河野信, 田島健次, 甲野裕之, 柴肇一, 藤原政司, 恵良田知樹, 棟方正信, 高井光男, セルロース学会年次大会講演要旨集, 7th, 94, 01 Jul. 2000
Japanese

酢酸菌βグルコシダーゼ遺伝子のクローニングとセルロース合成における働き
田島健次, 中島克敏, 河野信, 甲野裕之, 柴肇一, 藤原政司, 恵良田知樹, 棟方正信, 高井光男, セルロース学会年次大会講演要旨集, 7th, 60, 01 Jul. 2000
Japanese

酢酸菌セルロース生合成におけるセルラーゼの効果
河野信, 甲野裕之, 田島健次, 恵良田知樹, 高井光男, 日本化学会北海道支部研究発表会講演要旨集, 2000, 49, 21 Jan. 2000
Japanese

Interaction between poly(P) and RNA polymerase : Its effect on transcription
Tsutsumi Kaori, Shiba Toshikazu, Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 12, 23, 23, 2000
日本生物工学会, Japanese

Characterization of levansucrase from Acetobacter xylinum
Kobayashi Yasunobu, Tajima Kenji, Shiba Toshikazu, Erata Tomoki, Munekata Masanobu, Takai Mitsuo, 日本生物工学会大会講演要旨集, 12, 206, 206, 2000
日本生物工学会, Japanese

Expression regulation of a gene encoding 2-aminothiazoline-4-carboxylate racemase
Yajima Misako, Shiba Toshikazu, Takabatake Naoki, Takeda Kouji, Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 12, 221, 221, 2000
日本生物工学会, Japanese

Construction of novel ATP regeneration system using poly(P)-AMP phosphotransferase
Kameda Atsushi, Shiba Toshikazu, Satoh Yasuharu, Tajima Kenji, Munekata Masanobu, Ishige Kazuya, Noguchi Toshitada, 日本生物工学会大会講演要旨集, 12, 222, 222, 2000
日本生物工学会, Japanese

In vitro synthesis of P(HB-co-HV)
Satoh Yasuharu, Tajima Kenji, Tannai Hatsumi, Erata Tomoki, Shiba Toshikazu, Munekata Masanobu, 日本生物工学会大会講演要旨集, 12, 238, 238, 2000
日本生物工学会, Japanese

In vitro PHA synthesis with immobilized enzymes
Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 12, 358, 358, 2000
日本生物工学会, Japanese

固定化菌体・酵素を用いた生分解性プラスチックの連続合成
田島健次, 高井光男, 棟方正信, 柴肇一, 辻雅久, 田中昭子, 北海道の研究開発, 1999, 125, 134, Dec. 1999, [Invited]
Japanese

Induction of acetic acid bacteria CMCase and its influence on cellulose synthesis.
河野信, 甲野裕之, 田島健次, 恵良田知樹, 高井光男, 日本木材学会北海道支部講演集, 31, 61, 64, 15 Oct. 1999
Japanese

Function of cellulolytic enzymes in acetic acid bacteria biosynthesis and its effect.
甲野裕之, 河野信, 田島健次, 恵良田知樹, 高井光男, 日本木材学会北海道支部講演集, 31, 59, 60, 15 Oct. 1999
Japanese

セルロース学会第6回年次大会'99 Cellulose R&D レポート
藤原 政司, 田島 健次, Cellulose communications, 6, 2, 106, 110, Aug. 1999, [Invited]
セルロ-ス学会, Japanese

セルラーゼ存在下での酢酸菌によるセルロース合成 外部添加したセルラーゼと酢酸菌由来セルラーゼの影響
甲野裕之, 河野信, 田島健次, 藤原政司, 恵良田知樹, 高井光男, セルロース学会年次大会講演要旨集, 6th, 58, 59, Jun. 1999
Japanese

酢酸菌CMCaseのオリゴ糖による誘導とセルロース産生
河野信, 甲野裕之, 田島健次, 恵良田知樹, 高井光男, セルロース学会年次大会講演要旨集, 6th, 60, Jun. 1999
Japanese

セルラーゼ存在下での酢酸菌によるセルロース合成 セルラーゼ糖転移反応物の構造解析とセルロース生合成への影響
甲野裕之, 河野信, 田島健次, 藤原政司, 恵良田知樹, 高井光男, WEALCHILI M R, セルロース学会年次大会講演要旨集, 6th, 37, 38, Jun. 1999
Japanese

Development of biodegradable materials from bacterial cellulose.
田島健次, バイオサイエンスとインダストリー, 57, 6, 391, 394, Jun. 1999, [Invited]
バイオインダストリ-協会, Japanese

カルボキシメチルセルロース(CMC)によるバクテリアセルロース(BC)生産の増強
田島健次, Cellulose Communications, 6, 1, 18, 21, Apr. 1999, [Invited]
セルロ-ス学会, Japanese

Stabilization of FGF by inorganic polyphosphate.
Shiba Toshikazu, Nishimura Daisuke, Onodera Yuichiro, Ohshiro Minako, Nakamura Rie, Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 11, 33, 33, 1999
日本生物工学会, Japanese

Purification and biochemical characteristics of ATC racemase
Yajima Misako, Miyatake Masahiko, Matsumoto Nobutoshi, Takabatake Naoki, Tajima Kenji, Shiba Toshikazu, Munekata Masanobu, 日本生物工学会大会講演要旨集, 11, 39, 39, 1999
日本生物工学会, Japanese

In vitro PHB synthesis by immobilized enzyme
Tannai Hatsumi, Satoh Yasuharu, Tajima Kenji, Shiba Toshikazu, Munekata Masanobu, 日本生物工学会大会講演要旨集, 11, 65, 65, 1999
日本生物工学会, Japanese

Enzymatic synthesis of PHB from acetate
Satoh Yasuharu, Tannai Hatsumi, Tajima Kenji, Shiba Toshikazu, Munekata Masanobu, 日本生物工学会大会講演要旨集, 11, 66, 66, 1999
日本生物工学会, Japanese

Effect of CMCase on cellulose synthesis by acetic acid bacteria.
田島健次, 河野信, 藤原政司, 恵良田知樹, 棟方正信, 高井光男, 日本木材学会北海道支部講演集, 30, 57, 60, Oct. 1998
Japanese

酢酸菌のセルロース合成におけるグルコース脱水素酵素の影響
藤原政司, 俵敦, 田島健次, 高井光男, セルロース学会年次大会講演要旨集, 5th, 49, Jul. 1998
Japanese

酢酸菌CMCaseのセルロース合成における影響
河野信, 田島健次, 藤原政司, 恵良田知樹, 棟方正信, 高井光男, セルロース学会年次大会講演要旨集, 5th, 48, Jul. 1998
Japanese

Role of CMCase in the cellulose synthesis of acetobacter.
河野信, 田島健次, 藤原政司, 棟方正信, 高井光男, 日本化学会北海道支部研究発表会講演要旨集, 1998, 104, Feb. 1998
Japanese

Cellulose production by Acetobacter xylinun in the presence of cellulase　　　　　　　　　　　　　　　
T. Nakamura, K. Tajima, M. Fujiwara, M. Takai, J. Hayashi, Use of Minerals in Papermaking, 3, 8, 1998
The cellulose productivity by Acetobacter xylinum, cellulose producing bacteria, is enhanced by a small amount of heat-denatured cellulase. Effect of an endoglucanase, two exoglucanases and two β-D-glucosidases, purified from a commercial cellulase preparation of Trichoderma viride using anion exchange column Mono Q and gel filtration column Superdex 75, has been studied on cellulose production. The heat-denatured cellulase components showed a little enhancement of cellulose productivity compared with the standard culture. In the native state, the β-D-glucosidase fraction is the most effective component for cellulose production. These results suggest that enhancement of cellulose productivity by heat-denatured collulase mainly result from β-D-glucosidase component in the commercial cellulase., Pira Int, English, Introduction commerce magazine

Synthesis and characterization of bacterial cellulose composite (BCC)　　　　　　　　　　　　　　　
K. Tajima, M. Fujiwara, M. Takai, J. Hayashi, Use of Minerals in Papermaking, 9, 14, 1998
In general, biodegradability of a material decreases with an increase in its mechanical strength. Bacterial cellulose (BC) produced by Acetobacter xylinum (A. xylinum) is a very unique and interesting material, since it has naturally both high mechanical strength and biodegradability. We have succeeded in preparing an incorporated BC (BC composite) with water-soluble polymer, such as carboxymethyl cellulose (CMC) or methyl cellulose (MC) by adding water-soluble polymer into the standard medium of A. xylinum. Young's modulus of BC composites was about 1 - 3 times that of normal BC (30 - 40 GPa) and BC composites were almost degraded in soil after 28 days as well as normal BC. This preparation method has been applied to synthesize a new type of BC having both high biodegradability and other functions., Pira Int, English, Introduction commerce magazine

Cloning of ATC racemase gene and analysis of its activity.
矢島美彩子, 宮武正彦, 松本信俊, 柴肇一, 田島健次, 棟方正信, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 1998, 16, 1998
Japanese

polyP-AMP phosphotransferase activity in Myxococcus xanthus
Kameda Atsushi, Shiba Toshikazu, Ishige Kazuya, Noguchi Toshitada, Hoshino Chiharu, Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 10, 167, 167, 1998
日本生物工学会, Japanese

Cloning and Characterization of a Levansucrase from Acetobacter xylinum NCI1005
Tanio Toshiyuki, Tajima Kenji, Fujiwara Masasi, Erata Tomoki, Munekata Masanobu, Takai Mitsuo, 日本生物工学会大会講演要旨集, 10, 250, 250, 1998
日本生物工学会, Japanese

Inorganic polyphosphate involves in SOS regulon
Tsutsumi Kaori, Shiba Toshikazu, Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 10, 252, 252, 1998
日本生物工学会, Japanese

Effect of inorganic polyphosphate on cell growth
Nishimura Daisuke, Shiba Toshikazu, Oshiro Minako, Tajima Kenji, Munekata Masanobu, 日本生物工学会大会講演要旨集, 10, 342, 342, 1998
日本生物工学会, Japanese

Intracellular expression of levansucrase derived from acetic acid bacteria in Escherichia coli.
谷尾俊幸, 俵敦, 田島健次, 藤原政司, 高井光男, 日本化学会北海道支部研究発表会講演要旨集, 1997, 110, Feb. 1997
Japanese

Synthesis of water-soluble polysaccharide by acetic acid bacteria and structual analysis using NMR.
上西誠人, 田島健次, 藤原政司, 恵良田知樹, 高井光男, 日本化学会北海道支部夏季研究発表会講演要旨集, 1996, 35, 1996
Japanese

Its mechanical stregth and biodegradability
TAJIMA Kenji, FUJIWARA Masashi, TAKAI Mitsuo, HAYASHI Jisuke, Journal of the Japan Wood Researh Society, 41, 8, 749, 757, 25 Aug. 1995
日本木材学会, English

Synthesis of bacterial cellulose by rotating disc bioreactor.
本郷忠志, 田島健次, 藤原政司, 高井光男, 林治助, 高分子学会北海道支部研究発表会講演要旨集, 29th(1994), 23, Feb. 1995
Japanese

Culture of acetic acid bacteria in water-soluble polymer added culture medium.
田島健次, 藤原政司, 高井光男, 林治助, 日本化学会北海道支部夏季研究発表会講演要旨集, 1995, 23, 1995
Japanese

水溶性高分子を添加した培地における酢酸菌の培養
田島健次, 藤原政司, 高井光男, 林治助, 日本化学会北海道支部夏季研究発表会講演要旨集, 1994, 43, 1994
Japanese

微生物セルロース合成における培養方法の検討とバイオリアクターの考案
本郷忠志, 田島健次, 藤原政司, 高井光男, 林治助, 日本化学会北海道支部夏季研究発表会講演要旨集, 1993, 24, 1993
Japanese

酵素添加による微生物セルロースの合成
藤原政司, 中村励, 清水圭一, 田島健次, 高井光男, 林治助, 日本化学会講演予稿集, 64th, 2, 805, Sep. 1992
Japanese

Cellulose Biosynthesis by Acetobacter xylinum with Heat‐denatured Cellulase.
高井光男, 藤原政司, 清水圭一, 田島健次, 中村励, 林治助, 高分子学会予稿集, 41, 6, 2165, 2167, Sep. 1992
Japanese

酢酸菌による菌体外多糖の合成
田島健次, 藤原政司, 高井光男, 林治助, 日本化学会講演予稿集, 64th, 2, 806, Sep. 1992
Japanese

Synthesis of exo‐polysaccharide by Acetobacter.
田島健次, 藤原政司, 高井光男, 林治助, 高分子学会予稿集, 41, 6, 2162, 2164, Sep. 1992
Japanese

酢酸菌による菌体外多糖の合成
田島健次, 藤原政司, 高井光男, 林治助, 日本木材学会大会研究発表要旨集, 42nd, 361, Apr. 1992
Japanese

酢酸菌による水溶性多糖の合成とその構造について
田島健次, 藤原政司, 高井光男, 林治助, 日本化学会講演予稿集, 62nd, 1, 328, Sep. 1991
Japanese

セルロースナノファイバー : 研究と実用化の最前線
田島健次, 松島得雄, 小瀬亮太, 藤田彩華, 甲野裕之, 安藤英紀:石田竜弘, ナノフィブリル化バクテリアセルロースの生産とその応用
エヌ・ティー・エス, Nov. 2021, 9784860437510, 5, 15, 832, 15p, 図版22p, Japanese, [Contributor]

セルロースナノファイバー製造･利用の最新動向　　　　　　　　　　　　　　　
田島 健次, 発酵ナノセルロースの大量生産とその応用
シエムシー出版, 2019, [Contributor]

セルロースナノファイバーの均一分散と複合化　　　　　　　　　　　　　　　
田島 健次, 酢酸菌におけるセルロース合成機構と通気撹拌培養によるナノフィブリル化バクテリアセルロース（NFBC）の合成
サイエンス＆テクノロジー, 2018, [Contributor]

セルロースナノファイバーの調製、分散・複合化と製品応用　　　　　　　　　　　　　　　
田島 健次, 酢酸菌におけるセルロース合成機構と通気撹拌培養によるナノフィブリル化バクテリアセルロース（NFBC）の合成
技術情報協会, 2015, [Contributor]

バイオマス分解酵素の最前線－セルラーゼ・ヘミセルラーゼを中心として－　　　　　　　　　　　　　　　
田島 健次, セルロース合成における分解酵素の役割
シエムシー出版, 2012, [Contributor]

Cell Technology for Cell Products　　　　　　　　　　　　　　　
Nobuhiro Nagai, Shunji Yunoki, Takeshi Suzuki, Yasuharu Satoh, Kenji Tajima, Masanobu Munekata, Preparation of Salmon Atelocollagen Fibrillar Gel and Its Application to the Cell Culture
Springer, 2007, 9781402054761, 321-323, English, Scholarly book, [Contributor]

木質科学実験マニュアル 日本木材学会編　　　　　　　　　　　　　　　
田島 健次, タンパク質の分離，精製，定量
文永堂出版, Apr. 2000, [Contributor]

セルロースの事典<セルロース学会>　　　　　　　　　　　　　　　
田島 健次, セルロースの高次構造
朝倉書店, 2000, [Contributor]

Production and Applications of Bacterial Cellulose Nanofibers (BCNFs)　　　　　　　　　　　　　　　
Kenji Tajima
Fujihara International Symposium on Nanocellulose 2024, 03 Oct. 2024, English, Public discourse
30 Sep. 2024 - 03 Oct. 2024, [Invited]

Production and Applications of Bacterial Cellulose Nanofibers (BCNFs)　　　　　　　　　　　　　　　
Kenji Tajima
Green Inovation from Agriculture Field and Sustainable Life in Vietnam, 19 Sep. 2024, English, Nominated symposium
19 Sep. 2024 - 19 Sep. 2024, [Invited]

てん菜から作られる微⽣物セルロースナノファイバーの特⻑とその応⽤　　　　　　　　　　　　　　　
田島 健次
令和6 年度 てん菜多用途利用に関するセミナー, 26 Aug. 2024, Japanese, Public discourse
26 Aug. 2024 - 26 Aug. 2024, [Invited]

Characteristics Applications of Bacterial Cellulose Nanofibers (BCNFs)　　　　　　　　　　　　　　　
Kenji Tajima
Hokkaido University - National Central University Joint Symposium on Materials Chemistry and Physics 2024, 23 Aug. 2024, English, Nominated symposium
23 Aug. 2024 - 23 Aug. 2024, [Invited]

微生物を用いた機能性新素材の開発 ~人と環境にやさしい発酵ナノセルロースとは~　　　　　　　　　　　　　　　
田島 健次
北海道大学オープンキャンパス2024, 05 Aug. 2024, Japanese, Public discourse
05 Aug. 2024 - 05 Aug. 2024, [Invited]

バクテリアを用いたセルロースナノファイバーの 合成とそのキャラクタリゼーションおよび用途開発　　　　　　　　　　　　　　　
田島 健次
第31回セルロース学会年次大会, 11 Jul. 2024, Japanese, Invited oral presentation
11 Jul. 2024 - 11 Jul. 2024, [Invited]

新たな機能素材を生み出す微生物～環境に優しい発酵ナノセルロースとは？～　　　　　　　　　　　　　　　
田島健次
さっぽろ市民カレッジ２０２４冬期／ちえりあ学習ボランティア企画講座, 22 Mar. 2024, Japanese, Public discourse
[Invited]

微生物ナノセルロースを用いた 高強度環境循環型高分子材料の開発　　　　　　　　　　　　　　　
田島 健次
ロバスト最終報告会, 21 Mar. 2024, Japanese, Others
21 Mar. 2024 - 21 Mar. 2024, [Invited]

寒冷地輪作とてん菜由来の糖蜜から作られる微生物セルロースナノファイバーが世界を変える　　　　　　　　　　　　　　　
田島健次
令和５年度JAつべつ管内における各種実証・研究報告会, 19 Jan. 2024, Japanese, Keynote oral presentation
19 Jan. 2024 - 19 Jan. 2024, [Invited]

Development of Bacterial Cellulose Nano-Fiber;BCNF;and collaboration with companies　　　　　　　　　　　　　　　
Kenji Tajima
OPEN INNOVATION DAY - TECHTRAVERSE 2023, 26 Oct. 2023, English, Public discourse
[Invited]

Creation of high-strength composite materials using cellulose nanofibers with different fiber lengths　　　　　　　　　　　　　　　
Kenji Tajima
New Technology Briefing Session, 05 Oct. 2023, Japanese, Public discourse
[Invited]

バクテリア由来セルロースナノファイバーの構造・物性とその応用例　　　　　　　　　　　　　　　
田島 健次
ふじのくにセルロース循環経済国際展示会, 02 Oct. 2023, Japanese, Others
02 Oct. 2023 - 03 Oct. 2023, [Invited]

Bioorganic material (cellulose) synthesis using microorganisms in space　　　　　　　　　　　　　　　
Kenji Tajima
2023 Cellulose R&D, 29 Sep. 2023, Japanese, Oral presentation

Sugar beet, a key crop in cold-weather field rotations, and nanocellulose produced by microorganisms are changing the world.　　　　　　　　　　　　　　　
Kenji Tajima
Agri-Seeds Seminar "New Materials from Hokkaido of Plant Origin Opening the Way to the Future - From Seeds Development to Commercialization, 12 Sep. 2023, English, Nominated symposium
[Invited]

Microbial nanocellulose (NFBC), a new material from Hokkaido　　　　　　　　　　　　　　　
Kenji Tajima
The 15th Robust Agriculture, Forestry, and Fisheries Engineering "Leading Technology for Science and Technology Workshop"., 30 Aug. 2023, Japanese, Nominated symposium
[Invited]

Development and utilization of a completely recyclable new material (microbe-produced cellulose nanofiber) using sugar beet　　　　　　　　　　　　　　　
Kenji Tajima
Green Technobank Sugar Beet Research Association 21st Technical Research Presentation, 19 Jul. 2023, Japanese, Nominated symposium
[Invited]

Structure and Properties of Bacteria-derived Cellulose Nanofibers and Their Applications　　　　　　　　　　　　　　　
Kenji Tajima
Academic Forum@PharmaLab EXPO, 07 Jul. 2023, Japanese, Public discourse

北海道発の新素材 微生物産生セルロースナノファイバー (NFBC＝ファイブナノ®)の特長とその応用
田島健次
第7回農水産業支援技術展沖縄技術説明会, 07 Dec. 2022, Japanese, Public discourse
06 Dec. 2022 - 07 Dec. 2022, 34286926, [Invited]

Production and Applications of NanofibrillatedBacterial Cellulose (NFBC)
Kenji Tajima
Association of Bio-based Material Industry (ABbI) Webinar, 11 Oct. 2022, English, Nominated symposium
11 Oct. 2022 - 11 Oct. 2022, 34286926, [Invited]

High-yield Production of Nanofibrillated Bacterial Cellulose (NFBC) in Aerated and Stirred Bioreactors
Ryo Takahama, Masato Uenishi, Tokuo Matsushima, Takuya Isono, Toshifumi Satoh, Chiaki Ogino, Kenji Tajima
5th INTERNATIONAL SYMPOSIUM ON BACTERIAL CELLULOSE, 22 Sep. 2022, English, Oral presentation
22 Sep. 2022 - 23 Sep. 2022, 34286926

Production and applications of nanofibrillated bacterial cellulose
Kenji Tajima
5th INTERNATIONAL SYMPOSIUM ON BACTERIAL CELLULOSE, 22 Sep. 2022, English, Invited oral presentation
22 Sep. 2022 - 23 Sep. 2022, 34012611, [Invited]

北海道発の新素材微生物産生セルロースナノファイバーNFBC（Fibnano®）の特長とその応用
田島健次
ナノセルロースジャパンが開催するナノセルロース塾第五期（2022年度）, 17 Sep. 2022, Japanese, Public discourse
34286926, [Invited]

高アスペクト比を有する微生物由来セルロースナノファイバーを用いた 生分解性プラスチックの物性改良
Hamidah binti Hashim, Nur Aisyah Adlin, binti Emran, 勝原哲, 二之湯寛子, 辻崎晴人, 夏小超, 細川真明, 瀬野修一郎, 可児浩, 磯野拓也, 山本拓矢, 佐藤敏文, 折原宏, 田島健次
第71回高分子討論会, 06 Sep. 2022, Japanese, Nominated symposium
05 Sep. 2022 - 07 Sep. 2022, 34012607

Production and applications of nanofibrillated bacterial cellulose
Kenji Tajima
ICRI-BioM (International Centre for Research on Innovative Bio-based Materials) seminor, 01 Jul. 2022, English, Public discourse
34012607, [Invited]

酢酸菌セルロース合成酵素複合体の構造と機能（シンポジウム：酢酸菌ナノセルロース研究の最前線：合成の分子メカニズムと応用）　　　　　　　　　　　　　　　
田島健次
第73回日本生物工学会大会, 29 Oct. 2021, Japanese, Nominated symposium
[Invited]

北海道発の新素材微生物産生セルロー スナノファイバーFibnano®の特⾧とその応用　　　　　　　　　　　　　　　
田島 健次
北海道経済連合会 2020年度 環境・エネルギーセミナー, 18 Jan. 2021, Japanese, Public discourse
[Invited]

北海道発の新素材微生物産生セルロースナノファイバー Fibnano(R)の特長と食品応用　　　　　　　　　　　　　　　
田島 健次
CNF実用化事例紹介セミナー CNFによる機能付与の可能性および実現例, 05 Nov. 2020, Japanese, Public discourse
05 Nov. 2020, [Invited]

セルロース合成菌を用いたナノセルロースの大量調製とその構造を活かした応用　　　　　　　　　　　　　　　
田島 健次
2019 年度微粒子工学講演会, 28 Nov. 2019, Japanese, Nominated symposium
[Invited], [Domestic Conference]

In vitro cellulose synthesis using a recombinant cellulose synthase　　　　　　　　　　　　　　　
Kenji Tajima
4th International Symposium on Bacterial NanoCellulose, 03 Oct. 2019, English, Oral presentation
[International presentation]

Production of nanocellulose using a bacterium and its application　　　　　　　　　　　　　　　
田島 健次
2nd Asian-Franch Workshop on Polymer Science, 20 Jul. 2019, English, Invited oral presentation
[Invited], [International presentation]

Production of nanocellulose using a bacterium and its features　　　　　　　　　　　　　　　
TAJIMA Kenji
Sci-Mix in Kanazawa 2019, Green Innovative Chemistry, 07 Jun. 2019, English, Public discourse
[Invited], [Domestic Conference]

酢酸菌におけるセルロース合成メカニズムの解明と産業応用に向けた取り組み　　　　　　　　　　　　　　　
田島 健次
フジッコ株式会社社内講演会, 20 Mar. 2019, Japanese, Public discourse
[Invited], [Domestic Conference]

バクテリアを用いたナノセルロースの調製とその利用　　　　　　　　　　　　　　　
田島 健次
日本化学会ATP(Advanced Technology Program), 19 Mar. 2019, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

ボトムアップで創るナノセルロースの特長とその特徴を活かした応用　　　　　　　　　　　　　　　
田島 健次
とくしま高機能素材活用促進フォーラム, 31 Oct. 2018, Japanese, Public discourse
[Invited], [Domestic Conference]

バクテリアを用いたナノセルロースの大量生産とその応用　　　　　　　　　　　　　　　
田島 健次
高分子学会エコマテリアル研究会「バイオベースマテリアルをつくろう・つかおう」, 20 Jul. 2018, Japanese, Public discourse
[Invited], [Domestic Conference]

セルロース合成菌を用いたナノセルロースのボトムアップ合成とその応用　　　　　　　　　　　　　　　
田島 健次
セルロースナノファイバー in 東北, 18 Jul. 2018, Japanese, Public discourse
[Invited], [Domestic Conference]

バクテリアを用いた両親媒ナノセルロースの合成とその応用　　　　　　　　　　　　　　　
田島 健次
セルロース学会第25回年次大会, 06 Jul. 2018, English, Oral presentation
[Domestic Conference]

セルロース合成菌を用いたナノセルロースのボトムアップ合成とその応用　　　　　　　　　　　　　　　
田島 健次
島津製作所社内セミナー「セルロースナノファイバーの分析技術セミナー」, 04 Jul. 2018, Japanese, Public discourse
[Invited], [Domestic Conference]

セルロース合成菌を用いたナノフィブリル化セルロースの大量生産とその応用　　　　　　　　　　　　　　　
田島 健次
nano tech 2018 【特別シンポジウム】ナノセルロースの技術開発最前線, 15 Feb. 2018, Japanese, Public discourse
[Invited], [Domestic Conference]

One-step production of amphiphilic nano-fibrillated cellulose using a cellulose-producing bacterium　　　　　　　　　　　　　　　
TAJIMA Kenji
3rd International Symposium on Bacterial NanoCellulose, 16 Oct. 2017, English, Oral presentation
[International presentation]

ナノフィブリル化バクテリアセルロースの大量生産とその応用　　　　　　　　　　　　　　　
田島 健次
セルロース学会第24回年次大会, 13 Jul. 2017, Japanese, Oral presentation
[Domestic Conference]

バクテリアを用いたナノセルロースのボトムアップ生産　　　　　　　　　　　　　　　
田島 健次
第10回ナノセルロースフォーラム技術セミナー・意見交換会, 06 Jul. 2017, Japanese, Public discourse
[Invited], [Domestic Conference]

発酵ナノセルロース（NFBC）の事業化について　　　　　　　　　　　　　　　
田島 健次
セルロースナノファイバーサミット in 北海道, 17 Mar. 2017, Japanese, Public discourse
[Invited], [Domestic Conference]

低分子バイオマスを原料としたナノフィブリル化バクテリアセルロースの大量生産　　　　　　　　　　　　　　　
田島 健次
2016年度生物工学会北日本支部札幌シンポジウム「低炭素化社会に資する最先端バイオリファイナリー研究」, 02 Sep. 2016, Japanese, Public discourse
[Invited], [Domestic Conference]

酢酸菌セルロースの生合成機構と応用　　　　　　　　　　　　　　　
田島 健次
酢酸菌研究会, 10 Oct. 2015, Japanese, Public discourse
[Invited], [Domestic Conference]

Production and application of nano-fibrillated bacterial cellulose (NFBC)　　　　　　　　　　　　　　　
TAJIMA Kenji
2nd International Symposium on Bacterial NanoCellulose, 10 Sep. 2015, English, Invited oral presentation
[Invited], [International presentation]

ナノフィブリル化バクテリアセルロースの合成と応用　　　　　　　　　　　　　　　
田島 健次
日本木材学会バイオマス変換研究会夏季講演会「バイオマス変換の研究最前線」, 11 Jul. 2015, Japanese, Public discourse
[Invited], [Domestic Conference]

酢酸菌によるセルロース合成と発酵ナノセルロース(NFBC)の大量生産　　　　　　　　　　　　　　　
田島 健次
バイオインダストリー協会JBAバイオセミナーシリーズ”未来へのバイオ技術”勉強会, 14 Mar. 2014, Japanese, Public discourse
[Invited], [Domestic Conference]

酢酸菌によるセルロース合成と発酵ナノセルロース(NFBC)の大量生産　　　　　　　　　　　　　　　
田島 健次
新化学技術推進協会(JACI)エネルギー・資源技術部会バイオマス分科会講演会, 13 Mar. 2014, Japanese, Public discourse
[Invited], [Domestic Conference]

函館マリンバイオフォーラム＆フェスタ2012 函館　　　　　　　　　　　　　　　
田島 健次
函館マリンバイオフォーラム＆フェスタ2012 函館, 24 Jul. 2012, Japanese, Public discourse
[Invited], [Domestic Conference]

バクテリアを用いた高分子材料の合成　　　　　　　　　　　　　　　
田島 健次
函館工業高等専門学校 物質工学科 学科講演会, 2012, Japanese, Public discourse
[Invited], [Domestic Conference]

バクテリアを用いた非可食バイオマスからの高分子材料の合成　　　　　　　　　　　　　　　
田島 健次
道央バイオ研究交流会講演会, 2012, Japanese, Public discourse
[Invited], [Domestic Conference]

バクテリアによるバイオポリマーの合成と新規材料創製　　　　　　　　　　　　　　　
田島 健次
バイオ産業クラスター・フォーラム・シーズ公開会, 2010, Japanese, Public discourse
[Invited], [Domestic Conference]

微生物を使って砂糖からプラスチックや紙を作る　　　　　　　　　　　　　　　
田島 健次
日本化学会体験入学講演会, 2010, Japanese, Public discourse
[Invited], [Domestic Conference]

微生物を用いて再生可能資源からプラスチックや紙を作る　　　　　　　　　　　　　　　
田島 健次
リコー第４回材料フォーラム, 2008, Japanese, Public discourse
[Invited], [International presentation]

生体を用いた機能性高分子材料の合成　　　　　　　　　　　　　　　
田島 健次
新事業創出に係る技術講演会, 2008, Japanese
[Invited], [Domestic Conference]

生体を用いた機能性高分子材料の合成　　　　　　　　　　　　　　　
田島 健次
日本化学会北海道支部講演会, 2006, Japanese, Public discourse
[Invited], [Domestic Conference]

生体を用いた機能性高分子材料の合成　　　　　　　　　　　　　　　
田島 健次
高分子学会北海道支部高分子若手研究会, 2005, Japanese, Public discourse
[Invited], [Domestic Conference]

２段階バイオリアクター用いた生分解性プラスチックの連続合成　　　　　　　　　　　　　　　
田島 健次
平成11年度ノーステック財団研究開発支援事業成果発表会, 2002, Japanese, Public discourse
[Invited], [Domestic Conference]

固定化菌体・酵素を用いた生分解性プラスチックの連続合成　　　　　　　　　　　　　　　
田島 健次
平成8年度ホクサイテック財団研究開発支援事業成果発表会, 1999, Japanese, Public discourse
[Invited], [Domestic Conference]

道内石油・天然ガス採掘土壌からの環境浄化に寄与する菌分離と利用　　　　　　　　　　　　　　　
田島 健次
平成5年度ホクサイテック財団研究開発支援事業成果発表会, 1996, Japanese, Public discourse
[Invited], [Domestic Conference]

微生物が作る複合材料　　　　　　　　　　　　　　　
田島 健次
高分子学会北海道高分子若手研究会, Sep. 1995, Japanese, Public discourse
[Invited], [Domestic Conference]

生物資源化学特論　　　　　　　　　　　　　　　
北海道大学 大学院 総合化学院

生命分子化学特論　　　　　　　　　　　　　　　
北海道大学 大学院 総合化学院

応用化学学生実験Ⅲ　　　　　　　　　　　　　　　
北海道大学 工学部

物質変換工学　　　　　　　　　　　　　　　
北海道大学 工学部

生物化学工学　　　　　　　　　　　　　　　
北海道大学 工学部

生物学Ⅰ　　　　　　　　　　　　　　　
北海道大学 高等教育推進機構 総合教育部

Jun. 2020 - Present
Nanocellulose Japan　　　　　　　　　　　　　　　

糖鎖導入を鍵とする高機能バイオベース高分子材料の開発
科学研究費助成事業
Oct. 2021 - Mar. 2025
磯野拓也, 佐藤敏文, 田島健次
文部科学省, 北海道大学, Coinvestigator

Elucidation of accumulation mechanism of cellulose nanofibers at oil-in-water emulsion interface and its application for the production of a novel hollow nanoparticles
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2021 - 31 Mar. 2024
甲野 裕之, 田島 健次
酢酸菌が作るナノフィブリル化バクテリアセルロース(NFBC)は均一な繊維幅で繊維長が15 μm以上の超高アスペクト比な繊維としての特長を持つ。現在、医薬や生体へ応用可能なCNFとして注目される一方、さらなる機能化のための配向制御や構造体を構築する技術の欠如が課題として残っている。そこで申請者はNFBCを用いたピッカリング乳化が発現可能となれば、乳化剤としてだけでなく、球状の液液界面に沿ったNFBCの三次元組織化が達成できると考えた。そこで本申NFBCによるピッカリング乳化機構の解明と乳化現象を活用した新しい概念に基づく新規中空ナノ粒子の合成を目的とした。
本申請ではエマルジョン形成に伴うNFBC集合状態、ミセル粒径と安定化制御に関する新知見を取得し、中空ナノ粒子の構造設計にフィードバックすることで構造最適化を図る。製剤・樹脂との複合化による特性評価を通じ、その有用性を実証し、ナノ繊維ネットワークが作る多孔性ナノ粒子を創製する。
初年度は乳化技術の構築とそのメカニズムの解明について検討を行った。ホモジナイザーを用いてNFBC水分散液と中鎖脂肪酸トリグリセリドを用いてピッカリングエマルジョンを形成させ、油/水比、NFBC濃度、ホモジナイザー出力/照射時間等をパラメータとして安定な乳化条件とその油滴粒径制御を検討した。現時点では数日安定なピッカエマルジョンが得られておらず、NEBC表層の疎水性制御を実施した。主にシランカップリング剤を用いてナノ繊維構造を有する表層化学修飾法を構築することが可能となった。次年度はこれら表層化学修飾NFBCライブラリーを使用して、ピッカリング乳化技術の達成を目指す。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Tomakomai National College of Technology, 21K05175

再生可能多糖類植物由来プラスチックによる資源循環社会共創拠点
共創の場形成支援プログラム
Nov. 2021 - Mar. 2024
金沢大学, 北海道大学, 東海国立大学機構, 神戸大学, 三井住友信託銀行株式会社, 株式会社ダイセル, 草野作工株式会社, DSP五協フードケミカル株式会社, 日本乳化剤株式会社, マルハニチロ株式会社, 日東電工株式会社
本拠点では多糖類農業廃棄物を資源として捉え、プラスチックごみを生み出さないバイオプラスチック製品を用途に合わせてリデザインする。適切な量だけ生産し、使用後に回収して再生することで無駄なく使い続ける、バイオプラスチック循環プラットフォームを構築する。そのプラットフォームが、様々な世界地域へ、樹木の年輪のように拡大成長し、大量生産、大量廃棄による暗黒の未来を防ぐ。本プラットフォームの運用のため、多糖類農作物を持続的に生産可能な技術を社会実装し、プラスチック生産、消費、リサイクルに関するサプライチェーンを構築し、絶対的デカップリングを達成する。このような社会システム転換の実現のため、インパクトファイナンスを実践する金融機関が協力する。拠点の循環型プラスチック製品の社会実装により、企業と使用者の価値観が変わるイノベーションサイクルを回して、様々な問題解決につながるモデルを示す。これら5つのサイクルを、まるで協奏(共創)曲のように奏でることで、ポストコロナ社会を見据えた資源循環共創社会と「価値観のイノベーション」を実現し、人々を幸せにするSDGs目標達成に、スピード感を持って貢献する。
国立研究開発法人科学技術振興機構, 金沢大学, Coinvestigator

微生物ナノセルロースを用いた高強度環境循環型高分子材料の開発
未来社会創造事業
Oct. 2021 - Mar. 2024
田島健次, 折原宏, 甲野裕之, 小瀬亮太, 瀬野修一郎
国立研究開発法人科学技術振興機構, 北海道大学, Principal investigator

バイオ有機素材の宇宙リサイクルシステム開発
宇宙航空科学技術推進委託費
Oct. 2021 - Mar. 2024
五十嵐圭日子, 田島健次, 今井友也, 田仲 広明
文部科学省, 東京大学, Coinvestigator

Control of polymer structure by enzyme: structure-function relationship of cellulose synthase
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
01 Apr. 2019 - 31 Mar. 2023
今井 友也, 田島 健次, 姚 閔
酢酸菌のセルロース合成酵素複合体の主要構成サブユニットのうち、ABCDの4サブユニットが一つの複合体中に存在することを支持する生化学的データを得ることに成功した。またその複合体の中で、AB複合体とDタンパク質が直接相互作用している場合と、合成されたセルロース分子を介して間接的に相互作用している場合があることを実験的に証明した。以上から、これら二つの相互作用モードを使い分けることで酵素複合体の状態制御を行っている可能性を示した。さらにこのタンパク質複合体精製実験を進め、クライオ電子顕微鏡法による三次元構造解析への道筋をつけた。具体的には組換え大腸菌および酢酸菌の二つの系を用いて、His-tagあるいはStrep-tagを用いたアフィニティークロマトグラフィー、サイズ排除クロマトグラフィーによる精製条件検討を進めた。
Cサブユニットは、全長タンパク質の取り扱いが難しいことから、C末のβバレル型チャネル部分のタンパク質結晶化を進め、結晶化条件について目途をつけつつある。また、Mass Photometry法により、この部分構造が会合体を形成することを確認した。さらにCタンパク質の機能解析を進めるために、Cサブユニットの遺伝子欠損酢酸菌株を使った実験の条件最適化を進めた。
また機能解析を効率的に進めるために、A遺伝子の機能欠損酢酸菌株の作出を進めた。具体的にはCRISPR-Cas9システムを用いるために新たなプラスミドを構築した。現在このプラスミドにターゲット配列を導入し、ノックインによるA遺伝子欠損株の作出を進めている。さらにセルロース合成に関与している新たな遺伝子を探索するためにランダム変異実験を行い、候補となる遺伝子をいくつか特定することが出来た。これらについて機能解析を行うため、CRISPR-Cas9システムを用いた欠損株の作製を現在行っている。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Kyoto University, 19H00950

微生物産生ナノフィブリル化セルロースの精密構造解析と新規用途開発
コンソーシアム形成型 ロバスト農林水産工学研究プログラム
Jun. 2021 - Apr. 2022
田島健次, 折原宏, 瀬野修一郎, 細川真明
北海道大学 持続可能社会の実現に向けた 世界トップレベル研究推進・社会実装 「ロバスト農林水産工学国際連携研究教育拠点構想」, Principal investigator

Efficient anticancer drug delivery using a network structure of nanocellulose with a super high aspect ratio
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2019 - 31 Mar. 2022
Tajima Kenji
Nanofibrillated bacterial cellulose (NFBC) exhibits shear-shearing flow behavior, in which the viscosity decreases with increasing shear rate even at low concentrations, and its high aspect ratio is confirmed to form a network structure. Various surface modification methods, such as silane coupling and grafting, were investigated to add new functions to NFBC. As for the drug delivery, by supplementing the anticancer drug in the dense network structure, it was possible to suppress the toxicity and efficiently deliver the drug to cancer cells intraperitoneally.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 19H02549

カーボンニュートラルを目指した オール多糖ガス分離膜の開発　　　　　　　　　　　　　　　
社会還元加速プログラム（SCORE）大学推進型（拠点都市環境整備型）
Aug. 2021 - Mar. 2022
沼田ゆかり、田島健次、甲野裕之
JST, Coinvestigator

道産ナノセルロースの表層化学改質と超高強度材料への応用展開
研究開発助成事業 イノベーション創出研究支援事業発展・橋渡し研究補助金
Jul. 2019 - Mar. 2020
甲野裕之, 田島健次, 沼田ゆかり, 松島得雄
ノーステック財団, Competitive research funding

酸素移動容量係数の解析に基づく糖質からのナノセルロース生産の効率化
ロバスト農林水産工学研究プログラム
Jul. 2018 - Mar. 2020
田島健次, 佐藤信一郎
北海道大学, Principal investigator, Competitive research funding

発酵セルロースナノファイバーの表層化学修飾と複合材料への展開
研究開発助成事業イノベーション創出研究支援事業スタートアップ研究補助金
Jul. 2018 - Mar. 2019
甲野裕之, 田島健次, 沼田ゆかり, 松島得雄
ノーステック財団, Competitive research funding

Preparation and mechanism of the nano-fibrillated bacterial cellulose with molecular recognition ability
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Apr. 2016 - Mar. 2019
Kono Hiroyuki
Hydrophobic nano-fibrillated bacterial cellulose (NFBC) was prepared by use of an efficient silylation process in water. The starting material of NFBC was obtained by aerobic agitating cultivation of cellulose producing bacterium (Gluconacetobacter intermedius NEDO-01) in a medium supplemented with sodium carboxymethyl cellulose. The NFBC was heterogeneously silylated by methyltrimethoxysilane (MTMS) sols in the acidic water (pH4), and a series of the silylated NFBC was prepared by changing feed amount of MTMS sols. Structural characterization of these samples using FTIR, solid-state NMR, and WAXS revealed that the optimal condition for the selective silylation of the surface layer of NFBC. The optimally silylated NFBC was well dispersed in chloroform, and the nano-fibril structure was retained in the solvent.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Tomakomai National College of Technology, 16K05802

発酵ナノセルロース(NFBC)の効率的培養方法と分離精製技術の確立による量産化
戦略的基盤技術高度化支援事業
Aug. 2016 - Mar. 2018
松島得雄, 田島健次
経済産業省, Competitive research funding

バクテリアを用いたボトムアップ法による機能性ナノセルロースの創製
平成26年度 単年度研究助成
Apr. 2014 - Dec. 2015
田島 健次
公益財団法人 池谷科学技術振興財団, Principal investigator, Competitive research funding

函館マリンバイオクラスター～ＵＭＩ（Universal Marine Industry）のグリーンイノベーション　　　　　　　　　　　　　　　
地域イノベーション戦略支援プログラム
Apr. 2009 - Mar. 2014
高橋 はるみ
文部科学省, Competitive research funding

遺伝子工学およびバイオプロセス工学の応用による微細化バクテリアセ ルロースの大量生産と微細ネットワーク構造を利用した新規表示デバイスの開発
先導的産業技術創出事業（若手グラント）
Oct. 2011 - Sep. 2013
田島 健次
国立研究開発法人新エネルギー・産業技術総合開発機構(NEDO), Principal investigator, Competitive research funding

北海道産非可食原料(糖蜜)からのポリL‐乳酸改質に対応した高品質発酵D‐乳酸の量産技術の開発　　　　　　　　　　　　　　　
戦略的基盤技術高度化支援事業
Aug. 2009 - Mar. 2011
松島 得雄
経済産業省, Competitive research funding

生物工学的手法によるバイオポリマーの精密構造制御と化学修飾による機能化
科学研究費助成事業 基盤研究(B)
2009 - 2011
田島 健次, 佐藤 敏文, 佐藤 康治, 堺井 亮介
PHAは、微生物によって再生可能資源から作られるバイオポリマーである。これまでに、様々な構造・物性のPHAが作り出されてきているが、PHAに更なる機能性を付与するためには、新しい構造を有するモノマーユニットを導入する必要がある。本研究では、微生物を用いてまず反応点(官能性置換基)を導入し、その反応点を利用してモノマー構造を変化させ機能性PHAを合成する。研究としては、ベースとなる反応点を有するPHAの微生物合成とクリック反応によるPHAへの化学修飾・機能化と解析の2つに大きく分けることがきる。田島、佐藤康治に関してはPHAの微生物合成を、佐藤敏文、堺井については化学修飾・機能化およびその解析を担当する。平成21年度は、以下のことを行った。
(1) ポロヒドロキシアルカン酸(PHA)の微生物合成:PHA合成を欠失させた微生物(Ralstonia entropha PHB-4、Pseudomonas GPp104)にBacillus由来のPHA合成酵素遺伝子を導入した。ファーメンターを用い、基質をウンデシレン酸にすることによって側鎖に二重結合を有する短鎖長(scl-)PHAの合成に成功した。PHA中における二重結合の導入率は約5mol%程度であった。
(2) 側鎖に二重結合を有する中鎖長(mcl-)PHAに、クリック反応によってメルカプトプロピオン酸を導入した。NMR、FT-IR、GPC等の解析の結果から、ほぼ定量的に二重結合にメルカプトプロピオン酸が導入されていることが確認された。また、カルボキシル基の導入によって、ポリマーの溶解性が変化した。これらの結果から二重結合を有するPHAに対する分子団の導入においてクリック反応が有効であることが確認できた。
日本学術振興会, 基盤研究(B), 北海道大学, 21310060

セルロース合成酵素複合体の立体構造および機能の解析　　　　　　　　　　　　　　　
研究助成
Oct. 2008 - Mar. 2010
田島 健次
サッポロ生物科学振興財団, Principal investigator, Competitive research funding

Development of monomer-supplying enzymes for cell-free biopolyester production
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
2009 - 2010
SATOH Yasuharu, TAJIMA Kenji, HAN Xuerong
Polyhydroxyalkanoates (PHAs), which originate from renewable resources such as sugars and vegetable oils, have attracted great interest as alternatives to petrochemical-based plastics. To construct a novel cell-free PHA production system from sugars as feedstocks, an (R)-specific and NADH-dependent reductase is needed. In this study, we successfully developed the reductases to exchange stereo-selectivity of an (S)-specific enzyme. In addition, we could find the reductase from databases.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, 21760632

バイオディーゼル燃料（BDF）製造残渣を原料とする生分解性農業用マルチフィルムの開発試作　　　　　　　　　　　　　　　
イノベーション創出研究支援事業 重点研究・モデル化研究補助金
Jul. 2008 - Mar. 2009
吉田 美樹
ノーステック財団, Competitive research funding

Prroduction System for Carbon Recyclable Green Plastics Based on Enzyme Evolutionary Engineering
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2005 - 2007
TAGUCHI Seiichi, TAJIMA Kenji, IWATA Tadahisa, SHIMADA Hiroaki
Polyhydroxyalakanoates (PHAs) form a class of natural polyesters that many microorganisms in the environment accumulate in the form of intracellular granules to store carbon and reducing equivalents. The wide variety of monomers yields PHAs with diverse material properties that depend on polymer composition. Besides the practical applications of PHAs as bioplastics that are biodegradable and made from renewable resources, from the standpoint of an academic metabolic pathway engineer. PHAs are model compounds for metabolic engineering. The primary aims of the metabolic engineering of PHAs include controlling different factors that determine polymer material properties, such as monomeric compositions, molecular weight and copolymer microstructure, as well as optimizing yield. Pathway engineering for PHA production offers the opportunity to synthesize novel polymers with desirable properties in low-cost, high-productivity fermentations. Most recently, enzyme evolution is becoming the new approach for PHA production. A break-through in the chemical synthesis of macromolecules with desirable properties was achieved by the development of prominent chemical catalysts via "catalyst evolution". Thus, one can easily accept the concept that the molecular evolution of the biocatalysts (enzymes) relevant to PHA synthesis will provide us with a chance to create novel PHA materials with high performance. The enzyme evolution-aided PHA production system be involved in the carbon ecosystem. In this study, I demonstrated the case studies of enzyme engineering for optimization of productivity and regulation of monomeric composition of PHA. Rational molecular design was applied to monomer supplying enzyme based on three dimensional structure of PhaJ and evolutionary engineering was applied to PHA synthase based on functional mapping. A huge amount of library of enzyme mutants, created through engineering of these key enzymes for PHA biosynthesis, can provide tailor-made biopolymers with diverse properties suitable for wide-range applications. Transferring mutants to the plant system has been performed.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 17360392

バイオポリエステルの効率的生産系の開発と高機能化　　　　　　　　　　　　　　　
産業技術研究助成事
Oct. 2003 - Sep. 2006
田島 健次
国立研究開発法人新エネルギー・産業技術総合開発機構(NEDO), Principal investigator, Competitive research funding

バイオプラスチックの微生物から植物への高効率生産系開発　　　　　　　　　　　　　　　
基盤的研究開発育成事業 研究開発シーズ育成補助金
Jul. 2005 - Mar. 2006
田口 精一
ノーステック財団, Competitive research funding

皮コラーゲンを用いた人工歯根膜・ゲル培養歯根膜の開発　　　　　　　　　　　　　　　
産業創造技術研究開発支援事業 産業創造技術研究開発補助金
Jul. 2004 - Mar. 2006
棟方 正信
ノーステック財団, Competitive research funding

バイオポリマーの合成機構解明と遺伝子工学的手法を用いた新規機能性高分子の合成　　　　　　　　　　　　　　　
科学研究費補助金 若手研究(B)
Apr. 2002 - Mar. 2005
田島 健次
日本学術振興会, Principal investigator, Competitive research funding

Regeneration cascade in periodontal tissue and preparation of artificial periodontal ligaments
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
2002 - 2005
MUNEKATA Masanobu, TAJIMA Kenji, SATOH Yasuharu, NISHIMURA Kazuaki
1.Purification and characterization of human periodontal ligment (hPDL) cell chemotactic facter from bovine teeth precementum : We reported that the composite cementum particles-embedded gelatine membrane induced root surface mesenchymal cells to differentiated into cementoblasts and a promotive adjunct for true guided tissue regeneration (formoing new cementum, new ligment, and new bone) following periodontal flap surgery. The purpose of this sudy was to investigate that characterization of chemotactic factors released from cementum. They were purified from PBS(-)extracts of bovine teeth precementum using gel filtration-HPLC,DEAE and CM-ionexchange-HPLC, Affinity HPLC, and SDS-PAGE. After the purification by SDS-PAGE, extracts bands were analyzed TOF-MAS. We obtained two new subutances (named CDCF-I,II) which were not related attachment and growth protein such as BSP,CAP,PDGF-BB,IGF-I,TGF-β and fibronectin.
2.Effects of irradiation on cementum matrix cytokines function during periodontal regeneration : The influence of γ-ray irradiation of cementum (γC) was analyzed with emphasis on its function during periodontal regeneration. With γC protein, sample cells (gingival fibroblasts, periodontal ligament cells, and alveolar bone cells) were co-cultured, and cytological parameters were analyzed. Additionally, kinetics of some gene expression was analysed using reverse transcript RT-PCR, which induced osteoprotegerin (OPG)/osteoclastogenesis inhibitoryt factor (OCIF) mRNA. The expression of OPG/OCIF mRNA was lower in co-cultured cells with γC protein than those with in control C protein (non-irradiated cementum). Together the results imply that some cytokine in intact cementum prevents the attachment (differentiation) of bone cells onto the root surface, which may explain why the introduction of CGM (cementum-impregnated gelatine membrane) following gingival flap surgery induced new cementum, new ligament and new bone formation, but CGM irradiated with γrays for clinical use causes ankylosis.
3.New arifficial periodontal ligament from salmon atelocollagen : The fish collagen has lower risk for transmission of infectious diseases to human beings than bovine collagen. We therefore consider that fish collagen could be an alternative to bovine collagen for use as biomaterials. The purpose of this study was to investigate the application of salmon atelocollagen (SAC) to a scaffold. SAC has a low denaturation temperature (Td:19℃ and needs to be cross-linked before being used as a scaffold. SAC was cross-linked by EDC. The material properties of the SAC scaffolds cross-linked by EDC were evaluated. It was found that EDC-SAC had a high degree of cross-linking and high stability(Td:55℃). Human periodontal ligament (hPDL) cells were cultured in the scaffolds for 2 weeks in vitro, and the activities(proliferation rate and alkaline phosphatase activity (ALP) ) of hPDL cells cultured in EDC-SAC were compared with those cultured in bovine atelocollagen (BAC) scaffolds cross-linked by EDC (EDC-BAC). The proliferation rate of hPDL cells cultured in EDC-SAC was equivalent to that in EDC-BAC, and the ALP activity in EDC-SAC was found to be significantly higher than that in EDC-BAC. There was no difference in the thermal stability, porous structure, and cell proliferation rate between EDC-SAC and EDC-BAC. In addition, the collagen helix of EDC-SAC was found to be partially denatured, and this structure resulted in the enhancement of ALP activity of hPDL cells compared with that using EDC-BAC. Our results indicate that EDC-SAC could be used as a scaffold for regeneration of periodontal tissue.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), HOKKAIDO UNIVERSITY, 14208099

ナノポア制御による歯周組織再生能力を有する人工歯根膜の開発　　　　　　　　　　　　　　　
基盤的研究開発育成事業 研究開発シーズ育成補助金
Jul. 2003 - Mar. 2004
棟方 正信
ノーステック財団, Competitive research funding

セルロース合成に関与しているセルラーゼの立体構造解析とその機能解明　　　　　　　　　　　　　　　
奨励助成金
Apr. 2003 - Mar. 2004
田島 健次
秋山記念生命科学振興財団, Principal investigator, Competitive research funding

Analysis of Biosynthetic Pathways of Bacterial Cellulose for the Improvement of Cellulose Productivity
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2003 - 2004
FUJIWARA Masashi, TAKAI Mitsuo, MUNEKATA Masanobu, ERATA Tomoki, TAJIMA Kenji
The production cost of bacterial cellulose (BC) is too high to realize mass production for industrial materials. For its efficient production, the biosynthetic pathways of bacterial cellulose in Acetobacter xylinum were precisely examined by using culture media containing D-(1^<-13>C)glucose, (2^<-13>C)glucose or (6^<-13>C)glucose as the carbon source. Quantitative analysis of the NMR spectra of the glucose hydrolyzed from synthesized BC allows us to estimate the percentage of which metabolic pathway the fed glucose pass through. The results indicated that the rate of direct polymerization (DP) was 16%. The rate of the pentose phosphate cycle (PC) was 35%. The rate of the Entner-Doudoroff (ED) pathway was 41%. And the rate of the gluconeogenesis (GN) was 8%.
When the production of BC improved by ethanol addition, it is found that the rate of DP and GN were increased. In contrast, the rate of PC and ED were decreased. The results indicated that the glucose which does not pass through PC or ED could be used for BC production through DP. In the case of the excess addition of yeast extract, the rate of biosynthetic pathways was almost the same as in the case of ethanol addition. In this case, some contents in yeast extract might be used as energy source for growth of cells instead of glucose. And the glucose could be consumed as the source of cellulose production. In contrast, the improvement of BC production by lignin. sulfonate was considered to be caused by the different mechanisms because the increasement of DP was not observed.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), HOKKAIDO UNIVERSITY, 15550178

バイオポリマーの合成機構解明と遺伝子工学的手法を用いた新規機能性高分子の合成
科学研究費助成事業 若手研究(B)
2002 - 2004
田島 健次
セルロース生産菌Acetobacterxylinum由来のセルロース合成に関与するセルラーゼCMCaxの構造解析を目的として、酵素の単離精製、結晶化、および結晶構造解析を行った.この酵素は糖加水分解酵素のファミリー8(GH-8)に分類されており,すでに解析されていたGH-8に所属するセルラーゼCelA(Clostridium thermocellum由来)と相同な立体構造((α/α)6バレル構造)を形成していることが明らかになった.一方,糖鎖認識部はCelAにおいては6箇所の糖認識サブサイトを持つのに対して,CMCaxはひとつのチロシン残基が欠失していることからサブサイト+3が完全に消えており,この場所で認識クレフトが大きく開いていることが明らかになった.セルロース合成に関わる植物もしくは他のバクテリア由来のセルラーゼは,膜貫通ドメインをN末端側に持っており,膜にアンカーされた状態で細胞表面に存在すると考えられている.一方,CMCaxはN末端にシグナルペプチドを持つ細胞外分泌タンパク質であり,膜貫通ドメインは見られない.ところが,免疫染色法を用いた解析の結果,CMCaxも他のセルラーゼ同様に細胞表面に局在していることが明らかになった.これらの結果から,CMCaxは細胞表面において,合成され細胞から排出されるグルカン鎖と相互作用している可能性が示唆された.
日本学術振興会, 若手研究(B), 北海道大学, 14750688

遺伝子組換えによる高機能性セルロース誘導体のin vivo合成　　　　　　　　　　　　　　　
北海道大学クラーク記念財団助成事業 事業化研究助成
Apr. 2002 - Mar. 2003
田島 健次
北海道大学クラーク記念財団, Principal investigator, Competitive research funding

Development of artificial periodontal membranes for periodontal disease using polyphosphate
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
2000 - 2003
YAMAOKA Minoru, SHIBA Toshikazu, TANAKA Hitoshi, UEMATSU Takashi, ERATA Tomoki
Inorganic polyphosphates [Poly(P)] are chains of phosphate molecules and are often distributed in osteoblasts. The purpose of this study was to clarify the effects of Poly(P) on periodontal regeneration. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and its gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain reaction and von Kossa staining. Alkaline phosphatase activity was also determined. The potential role of Poly(P) in bone formation was assessed using a rat alveolar bone regeneration model. Poly(P) exhibited antibacterial activity against Prophyromonas gingivalis and Streptococcus mutans, induced expression of osteopontin and osteocalcine and increased alkaline phosphatase activity in MC 3T3-E1 cells. Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays roles of osteoblastic differentiation, activation and bone mineralization and possesses antibacterial activity. Thus, local delivery of poly(P) may have adjunctive therapeutic benefits in periodontal disease.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), 12558099

2段階バイオリアクターを用いた生分解性プラスチックの連続合成　　　　　　　　　　　　　　　
基盤的研究開発育成事業 研究開発シーズ育成補助金
Jul. 2001 - Mar. 2002
田島 健次
ノーステック財団, Principal investigator, Competitive research funding

セルロース合成に関与しているセルラーゼの立体構造解析とその機能解明　　　　　　　　　　　　　　　
北海道大学クラーク記念財団助成事業 独創的研究助成
Apr. 2001 - Mar. 2002
田島 健次
北海道大学クラーク記念財団, Principal investigator, Competitive research funding

固定化菌体・酵素を用いた生分解性プラスチックの連続合成　　　　　　　　　　　　　　　
基盤的研究開発育成事業 研究開発シーズ育成補助金
Jul. 1999 - Mar. 2000
田島 健次
ノーステック財団, Principal investigator, Competitive research funding

遺伝子組換えによる高機能性セルロース誘導体のin vivo合成　　　　　　　　　　　　　　　
研究助成
Apr. 1999 - Mar. 2000
田島 健次
ゼネラル石油研究医奨励財団, Principal investigator, Competitive research funding

歯プレセメント質由来糖タンパク質の構造と機能の解明　　　　　　　　　　　　　　　
科学研究費補助金 萌芽研究
Apr. 1997 - Mar. 1999
棟方 正信
日本学術振興会, Competitive research funding

固定化菌体を用いた生分解性プラスチックの連続合成　　　　　　　　　　　　　　　
産業化研究開発支援事業 研究開発産業化促進補助金
Apr. 1997 - Mar. 1999
田島 健次
ノーステック財団, Principal investigator, Competitive research funding

Development of High Performance Temporarily Artificial Skin made from Salmon Collagen and Squid Chitin
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
1997 - 1999
TAKAI Mitsuo, SHIBA Toshikazu, ERATA Tomoki, MUNEKATA Masanobu, TAJIMA Kenji, FUJIWARA Masashi
The squid chitin sheet prepared from the squid chitin suspension by air-decompression was used as the base sheet for temporarily artificial skin. The squid chitin sheet was laminated with salmon collagen for the growth of human skin cells. The chitin sheet laminated with salmon collagen showed clearly the superior attachment of human skin cells to the chitin sheet only as well as the chitin sheet laminated with the calf collagen. The growth of human skin cells on the chitin sheet laminated with salmon collagen was activated 1.3 times than that on the sheet laminated with calf collagen and 1.5 times than that on the chitin sheet only. The growing cells on the chitin sheet laminated with salmon collagen were confirmed as the normal cells by the DNA labeling index method. The chitin sheet laminated with salmon collagen showed the high bio-compatibility on the several bio-safety tests with the mouse or the rabbit. The continuous paper machine was developed for the manufacturing of the temporarily artificial skin from the squid chitin and the salmon collagen.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, 09555294

歯プレセメント質由来糖タンパク質の構造と機能の解明
科学研究費助成事業 萌芽的研究
1997 - 1998
棟方 正信, 田島 健次, 柴 肇一
本年度は,昨年度報告したヒト歯肉線維芽細胞を走化させるウシ歯根表面プレセメント質由来66KDaタンパクを,HPLCゲル濾過,HPLC陰イオン交換,HPLCハイドロキシアパタイト,HPLC-逆相,SDS-PAGE等にて多量に単離精製し,その走化活性及び増殖活性について既知物質との相違を検討した。歯周組織に存在する歯周靭帯細胞及び歯肉線維芽細胞に対し細胞走化活性がすでに報告されているPDGF-BB,AB,AA等に比べ,66kDaタンパクは約1/100の濃度で歯肉線維芽細胞に対し細胞走化活性を示したが,細胞増殖活性はPDGF-BB,AB,AA等の約10倍濃度を必要とした。すなわち66kDaタンパクは細胞走化活性に特異性がある物質と考えられた。またPDGF-BBとの併用では走化活性の相加効果が認められ,すでに前臨床での効果が報告されているPDGF-BBとIGF-Iの組み合わせより高い走化活性を示し,臨床応用が期待できる結果を得た。
γ線照射したウシ歯セメント質粒子をゼラチン多孔性膜に包埋し,歯肉剥離掻爬手術(in vivo:サル)に用いた場合,歯周靭帯形成がなされず歯槽骨再生のみが進行し,歯槽骨が歯根面に接着する,いわゆるアンキロージスが認められた。γ線照射したウシ歯セメント質粒子のPBS(-)抽出物のHPLCゲル濾過では,全体に抽出物の低分子化認められた。またSDS-PAGEでは,66kDaタンパクは認められなかったことから,66kDaタンパクは歯周靭帯形成(分化)にも関与している可能性が示唆された。
日本学術振興会, 萌芽的研究, 北海道大学, 09875238

Biosynthesis and Properties Control of Bacterial Cellulose
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
1996 - 1997
TAKAI Mitsuo, TAJIMA Kenji, FUJIWARA Masashi, SHIBA Toshikazu, ERATA Tomoki, MUNEKATA Masanobu
Bacterial cellulose (BC) productivity has been enhanced by the presence of 3.5% concentration of yeast extract in the medium as high as twice of the standard culture(0.5% yeast extract). Cell growth increased markedly with an increase in yeast extract concentration. pH of the medium decreased from the initial 5.9 to the range of 3.5-4.5 during 2 days incubation, however, after the prolonged incubation gradual increasing in pH was observed. Glucose as a carbon source was nevertheless consumed thoroughly during 4 days incubation, cellulose production proceeded slowly as long as 15 days incubation. An excess of yeast extract in the medium appear to play an role in the activator for cellulose biosynthesis. Bacterial cellulose (NBC) produced by Acetobacter xylinum (A.xylinum) has both great mechanical strength and biodegradability.The properties were controlled by the incorporation of water-soluble polymers (WSP) such as carboxymethyl cellulose (CMC), methyl cellulose (MC) and polyethylene glycol (PEG). Small amounts of the WSPs were added to the standerd Hestrin-Schramm medium.The WSPs were incorporated into BC during incubation. The cellulose composite (BCC) will be a kind of interpenetrating polymer.The BCC yield by weight were 1.5 times for MC and 1.8 times for CMC as compared with the weight of NBC control. The contents of MC and CMC in the BCC were checked by elemental analysis of C%, 45% for CMC and 20% for MC,respectively. From the X-ray analysis, the diffraction of (110) appeared sharply as a resuli of the disorientation of (110). The WSPs influence the preferential orientation of BC.Young's modulus of the BCC was about 1-3 times that of NBC (30-40GPa). BCC (CMC) was degraded completely by cellulase for 6 hr, while only 20-40% of BCC (MC) was degraded for 48hr. For degadation test in native soil, all BCC samples were degaded considerably at 30ﾟC for 4 weeks as well as NBC.There is some possibility to control a biodegadability of BC.The method of BCC production will be used to synthesize a new type of BC having both excellent biodegradability and other functions. The most effective incubation methods for NBC production were examined in various mode of bioreactor, and then on the basis of the results, the bioreactor with rotating-disk has been designed.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, 08455445

Reactor Design for Biocellulose Production to the Aims of Industrial Process
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
1995 - 1996
TAKAI Mitsuo, TAJIMA Kenji, FUJIWARA Masashi, SHIBA Toshikazu, ERATA Tomoki, MUNEKATA Masanobu
In this study, the most effective incubation methods for biocellulose production were examined in various mode of bioreactor, and then on the basis of the results, the bioreactor with rotating-disk has been designed. This is composed of horizontal pyrexcylinder of 20cm long and 15cm diameter, and stainless-made side plates equiped with one side of motor-drive, openings for culture medium and aeration, and with another side of pH and oxygen electrodes, two valves. Warmed water is passed through a thermojacked under the cylinder maintained at 28(]SY+-[)1ﾟC.pH in culture medium was automatically controlled by 1% NaOH aqueous solution. Aeration was done only into gas-phase by the filtered-air. Initial volume of the medium is 1800ml, that is half volume of the cylinder. Stainless mesh-disk from 6 to 16mesh of 14.4cm diameter were used as a supporter for bacterial cells. 60ml of preculture was inoculated into the reactor horizontally rotating 2-5rpm at 28ﾟC,and then incubated for 6days with aeration of 2000ml/min. The incubation was repeated 9times. Then total yealds of biocellulose has been examined for rotating speed and number of the disk, or pH control and aeration conditions. The most effective incubation conditions for the reactor of biocellulose production were as follows. 14mesh stainless disk was the most effective supporter for bacterial cells. Although the bioreactor produced about the same amount of biocellulose with respect to rotating speed of the disk, 30 and 50rpm, in the case of 50rpm incubation, there was some scattering of biocellulose too fast to retain on the disk. So the optimum rotating speed is 30rpm. Aeration into gas-phase would contribute to produce more biocellulose and to enhance physical properties of the biocellulose, especially, to increase in Young's modulus of the sheets.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), HOKKAIDO UNIVERSITY, 07555674

セルロースとヒアルロン酸との複合材、これを生産する細菌およびこれを製造する方法
Patent right, 近藤 哲男, 田島 健次, 近藤 哲男, 田島 健次, 草野作工株式会社
特願2019-190021, 17 Oct. 2019
特開2021-062167, 22 Apr. 2021
特許7454800, 14 Mar. 2024
202103008118279818

Bacterial cellulose and bacterium producing it　　　　　　　　　　　　　　　
Patent right, Kenji Tajima, Ryota Kose
Canada patent CA2896699C, 09 Nov. 2021
09 Nov. 2021, Kenji Tajima;Ryota Kose

バクテリアセルロースと樹脂との複合糸およびその製造方法
Patent right, 田島 健次, 草野 貴友, 草野 量文, 国立大学法人北海道大学, 草野作工株式会社
特願2018-124012, 29 Jun. 2018
特開2020-002504, 09 Jan. 2020
202003021102382014

ブロック共重合体及びこれを含む樹脂組成物、並びにブロック共重合体の製造方法
Patent right, 佐藤 敏文, 田島 健次, 磯野 拓也, 小林 新吾, 佐藤 俊輔, 国立大学法人北海道大学, 株式会社カネカ
特願2016-256838, 28 Dec. 2016
特開2018-109103, 12 Jul. 2018
201803004755447960

分散性バクテリアセルロース乾燥物の製造方法およびバクテリアセルロース分散液の製造方法
Patent right, 松島 得雄, 田島 健次, 草野作工株式会社
JP2017029798, 21 Aug. 2017
WO2018-038055, 01 Mar. 2018
WO2018-038055, 01 Mar. 2018
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Bacterial cellulose and bacteriu, producing same
Patent right, Kenji Tajima, Ryota Kose
European patent EP2940145
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Bacterial cellulose and bacterium producing it
Patent right, Kenji Tajima, Ryota Kose
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Bacterial cellulose and bacterium producing same
Patent right, Kenji Tajima, Ryota Kose
Australian patent AU2013366934
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Bacterial cellulose and bacterium producing it
Patent right, Kenji Tajima, Ryota Kose
US patent US9611495
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新規のアルギン酸資化菌、その細菌が産生するアルギン酸を分解する酵素を含む菌抽出液、それらを用いてオリゴ糖、不飽和単糖、ないしα−ケト酸を製造する方法
Patent right, 尾島 孝男, 田島 健次, 小林 孝紀, 国立大学法人北海道大学, 公益財団法人函館地域産業振興財団
特願2012-067226, 23 Mar. 2012
特開2012-210208, 01 Nov. 2012
特許第6025018号, 21 Oct. 2016
21 Oct. 2016
201603003667727782

Bacterial cellulose and bacterium producing same
Patent right, Kenji Tajima, Ryota Kose
Indian patent 4478/CHENP/2015
01 Jul. 2016

バクテリアセルロースおよびこれを生産する細菌
Patent right, 田島 健次, 小瀬 亮太, 櫻井 博章, 田島 健次, 小瀬 亮太, 日本甜菜製糖株式会社
特願2014-551859, 27 Dec. 2013
特許第5752332号, 29 May 2015
29 May 2015
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組み換え微生物を用いた共重合ポリエステルの製造方法
Patent right, 田口 精一, 田島 健次, 佐藤 康治, 松本 謙一郎, 山田 美和, 小畑 充生, 神戸 浩美, 幸田 勝典, 大野 克博, 嶋村 隆, トヨタ自動車株式会社
特願2010-509222, 23 Apr. 2009
特許第5625905号, 10 Oct. 2014
10 Oct. 2014
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共重合樹脂組成物、成形品、及び共重合樹脂組成物の製造方法
Patent right, 山中 康生, 原田 忠克, 柳▼沼 秀和, 棟方 正信, 田島 健次, 佐藤 康治, 松島 得雄, 株式会社リコー
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特開2011-032457, 17 Feb. 2011
特許第5577764号, 18 Jul. 2014
18 Jul. 2014
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バクテリアセルロースおよびこれを生産する細菌
Patent right, 田島 健次, 小瀬 亮太, 櫻井 博章, 田島 健次, 小瀬 亮太, 日本甜菜製糖株式会社
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熱可塑性樹脂の成形方法及び成形品
Patent right, 原田 忠克, 山中 康生, 柳▼沼 秀和, 棟方 正信, 田島 健次, 佐藤 康治, 松島 得雄, 株式会社リコー
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特開2010-142986, 01 Jul. 2010
特許第5556010号, 13 Jun. 2014
13 Jun. 2014
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樹脂組成物の成形方法及び成形品
Patent right, 柳▼沼 秀和, 原田 忠克, 山中 康生, 棟方 正信, 田島 健次, 佐藤 康治, 松島 得雄, 株式会社リコー
特願2008-320153, 16 Dec. 2008
特開2010-142985, 01 Jul. 2010
特許第5556009号, 13 Jun. 2014
13 Jun. 2014
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ポリヒドロキシアルカノエートの製造方法
Patent right, 佐藤 康治, 田島 健次, 棟方 正信, 松島 得雄, 日立化成株式会社
特願2008-012454, 23 Jan. 2008
特開2009-171883, 06 Aug. 2009
特許第5555975号, 13 Jun. 2014
13 Jun. 2014
201403034597447652

樹脂組成物及びこれを用いた成形品
Patent right, 山中 康生, 原田 忠克, 柳▼沼 秀和, 棟方 正信, 田島 健次, 佐藤 康治, 松島 得雄, 株式会社リコー
特願2008-320155, 16 Dec. 2008
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