Microbial Production of Human Milk Oligosaccharides.
Dileep Sai Kumar Palur, Shannon R Pressley, Shota Atsumi
Molecules (Basel, Switzerland), 28, 3, 03 Feb. 2023, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Human milk oligosaccharides (HMOs) are complex nonnutritive sugars present in human milk. These sugars possess prebiotic, immunomodulatory, and antagonistic properties towards pathogens and therefore are important for the health and well-being of newborn babies. Lower prevalence of breastfeeding around the globe, rising popularity of nutraceuticals, and low availability of HMOs have inspired efforts to develop economically feasible and efficient industrial-scale production platforms for HMOs. Recent progress in synthetic biology and metabolic engineering tools has enabled microbial systems to be a production system of HMOs. In this regard, the model organism Escherichia coli has emerged as the preferred production platform. Herein, we summarize the remarkable progress in the microbial production of HMOs and discuss the challenges and future opportunities in unraveling the scope of production of complex HMOs. We focus on the microbial production of five HMOs that have been approved for their commercialization.

Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria.
Shunichi Kobayashi, Shota Atsumi, Kazunori Ikebukuro, Koji Sode, Ryutaro Asano
Microbial cell factories, 21, 1, 7, 7, 06 Jan. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Cyanobacteria are engineered via heterologous biosynthetic pathways to produce value-added chemicals via photosynthesis. Various chemicals have been successfully produced in engineered cyanobacteria. Chemical inducer-dependent promoters are used to induce the expression of target biosynthetic pathway genes. A chemical inducer is not ideal for large-scale reactions owing to its high cost; therefore, it is important to develop scaling-up methods to avoid their use. In this study, we designed a green light-inducible alcohol production system using the CcaS/CcaR green light gene expression system in the cyanobacterium Synechocystis sp. PCC 6803 (PCC 6803). RESULTS: To establish the green light-inducible production of isobutanol and 3-methyl-1-butanol (3MB) in PCC 6803, keto-acid decarboxylase (kdc) and alcohol dehydrogenase (adh) were expressed under the control of the CcaS/CcaR system. Increases in the transcription level were induced by irradiation with red and green light without severe effects on host cell growth. We found that the production of isobutanol and 3MB from carbon dioxide (CO2) was induced under red and green light illumination and was substantially repressed under red light illumination alone. Finally, production titers of isobutanol and 3MB reached 238 mg L-1 and 75 mg L-1, respectively, in 5 days under red and green light illumination, and these values are comparable to those reported in previous studies using chemical inducers. CONCLUSION: A green light-induced alcohol production system was successfully integrated into cyanobacteria to produce value-added chemicals without using expensive chemical inducers. The green light-regulated production of isobutanol and 3MB from CO2 is eco-friendly and cost-effective. This study demonstrates that light regulation is a potential tool for producing chemicals and increases the feasibility of cyanobacterial bioprocesses.

Adaptive laboratory evolution for improved tolerance of isobutyl acetate in Escherichia coli.
Morgan M Matson, Mateo M Cepeda, Angela Zhang, Anna E Case, Erol S Kavvas, Xiaokang Wang, Austin L Carroll, Ilias Tagkopoulos, Shota Atsumi
Metabolic engineering, 69, 50, 58, Jan. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Previously, Escherichia coli was engineered to produce isobutyl acetate (IBA). Titers greater than the toxicity threshold (3 g/L) were achieved by using layer-assisted production. To avoid this costly and complex method, adaptive laboratory evolution (ALE) was applied to E. coli for improved IBA tolerance. Over 37 rounds of selective pressure, 22 IBA-tolerant mutants were isolated. Remarkably, these mutants not only tolerate high IBA concentrations, they also produce higher IBA titers. Using whole-genome sequencing followed by CRISPR/Cas9 mediated genome editing, the mutations (SNPs in metH, rho and deletion of arcA) that confer improved tolerance and higher titers were elucidated. The improved IBA titers in the evolved mutants were a result of an increased supply of acetyl-CoA and altered transcriptional machinery. Without the use of phase separation, a strain capable of 3.2-fold greater IBA production than the parent strain was constructed by combing select beneficial mutations. These results highlight the impact improved tolerance has on the production capability of a biosynthetic system.

Synthetic Biology Approaches for Improving Chemical Production in Cyanobacteria.
Tanner R Treece, Jake N Gonzales, Joseph R Pressley, Shota Atsumi
Frontiers in bioengineering and biotechnology, 10, 869195, 869195, 2022, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Biological chemical production has gained traction in recent years as a promising renewable alternative to traditional petrochemical based synthesis. Of particular interest in the field of metabolic engineering are photosynthetic microorganisms capable of sequestering atmospheric carbon dioxide. CO2 levels have continued to rise at alarming rates leading to an increasingly uncertain climate. CO2 can be sequestered by engineered photosynthetic microorganisms and used for chemical production, representing a renewable production method for valuable chemical commodities such as biofuels, plastics, and food additives. The main challenges in using photosynthetic microorganisms for chemical production stem from the seemingly inherent limitations of carbon fixation and photosynthesis resulting in slower growth and lower average product titers compared to heterotrophic organisms. Recently, there has been an increase in research around improving photosynthetic microorganisms as renewable chemical production hosts. This review will discuss the various efforts to overcome the intrinsic inefficiencies of carbon fixation and photosynthesis, including rewiring carbon fixation and photosynthesis, investigating alternative carbon fixation pathways, installing sugar catabolism to supplement carbon fixation, investigating newly discovered fast growing photosynthetic species, and using new synthetic biology tools such as CRISPR to radically alter metabolism.

Microbial production of human milk oligosaccharide lactodifucotetraose.
Angela Zhang, Lei Sun, Yuanyuan Bai, Hai Yu, John B McArthur, Xi Chen, Shota Atsumi
Metabolic engineering, 66, 12, 20, Jul. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Human milk oligosaccharides (HMOs) are potent bioactive compounds that modulate neonatal health and are of interest for development as potential drug treatments for adult diseases. The potential of these molecules, their limited access from natural sources, and difficulty in large-scale isolation of individual HMOs for studies and applications have motivated the development of chemical syntheses and in vitro enzymatic catalysis strategies. Whole cell biocatalysts are emerging as alternative self-regulating production platforms that have the potential to reduce the cost for enzymatic synthesis of HMOs. Whole cell biocatalysts for the production of short-chained, linear and small monofucosylated HMOs have been reported but those for fucosylated structures with higher complexity have not been explored. In this study, we established a strategy for producing a difucosylated HMO, lactodifucotetraose (LDFT), from lactose and L-fucose in Escherichia coli. We used two bacterial fucosyltransferases with narrow acceptor selectivity to drive the sequential fucosylation of lactose and intermediate 2'-fucosyllactose (2'-FL) to produce LDFT. Deletion of substrate degradation pathways that decoupled cellular growth from LDFT production, enhanced expression of native substrate transporters and modular induction of the genes in the LDFT biosynthetic pathway allowed complete conversion of lactose into LDFT and minor quantities of the side product 3-fucosyllactose (3-FL). Overall, 5.1 g/L of LDFT was produced from 3 g/L lactose and 3 g/L L-fucose in 24 h. Our results demonstrate promising applications of engineered microbial biosystems for the production of multi-fucosylated HMOs for biochemical studies.

Nonphotosynthetic Biological CO2 Reduction.
Jake N Gonzales, Morgan M Matson, Shota Atsumi
Biochemistry, 58, 11, 1470, 1477, 19 Mar. 2019, [Peer-reviewed], [Invited], [Last author], [International Magazine]
English, Scientific journal, Alarming changes in environmental conditions have prompted significant research into producing renewable commodities from sources other than fossil fuels. One such alternative is CO2, a determinate greenhouse gas with historically high atmospheric levels. If sequestered, CO2 could be used as a highly renewable feedstock for industrially relevant products and fuels. The vast majority of atmospheric CO2 fixation is accomplished by photosynthetic organisms, which have unfortunately proven difficult to utilize as chassis for industrial production. Nonphotosynthetic CO2 fixing microorganisms and pathways have recently attracted scientific and commercial interest. This Perspective will review promising alternate CO2 fixation strategies and their potential to supply microbially produced fuels and commodity chemicals, such as higher alcohols. Acetogenic fermentation and microbial electrosynthesis are the primary focuses of this review.

Metabolic engineering tools in model cyanobacteria.
Austin L Carroll, Anna E Case, Angela Zhang, Shota Atsumi
Metabolic engineering, 50, 47, 56, Nov. 2018, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Developing sustainable routes for producing chemicals and fuels is one of the most important challenges in metabolic engineering. Photoautotrophic hosts are particularly attractive because of their potential to utilize light as an energy source and CO2 as a carbon substrate through photosynthesis. Cyanobacteria are unicellular organisms capable of photosynthesis and CO2 fixation. While engineering in heterotrophs, such as Escherichia coli, has result in a plethora of tools for strain development and hosts capable of producing valuable chemicals efficiently, these techniques are not always directly transferable to cyanobacteria. However, recent efforts have led to an increase in the scope and scale of chemicals that cyanobacteria can produce. Adaptations of important metabolic engineering tools have also been optimized to function in photoautotrophic hosts, which include Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9, 13C Metabolic Flux Analysis (MFA), and Genome-Scale Modeling (GSM). This review explores innovations in cyanobacterial metabolic engineering, and highlights how photoautotrophic metabolism has shaped their development.

Electrical-biological hybrid system for CO2 reduction
Yohei Tashiro, Shinichi Hirano, Morgan M. Matson, Shota Atsumi, Akihiko Kondo
Metabolic Engineering, 47, 211, 218, 01 May 2018, [Peer-reviewed]
English, Scientific journal

Photomixotrophic chemical production in cyanobacteria
Matson Morgan M, Atsumi Shota
CURRENT OPINION IN BIOTECHNOLOGY, 50, 65, 71, Apr. 2018, [Peer-reviewed]

Systematic Approaches to Efficiently Produce 2,3-Butanediol in a Marine Cyanobacterium
Nicole E. Nozzi, Anna E. Case, Austin L. Carroll, Shota Atsumi
ACS SYNTHETIC BIOLOGY, 6, 11, 2136, 2144, Nov. 2017, [Peer-reviewed]
English, Scientific journal

Carbon recycling by cyanobacteria: improving CO2 fixation through chemical production
Angela Zhang, Austin L. Carroll, Shota Atsumi
FEMS MICROBIOLOGY LETTERS, 364, 16, Aug. 2017, [Peer-reviewed]
English

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria
Masahiro Kanno, Austin L. Carroll, Shota Atsumi
NATURE COMMUNICATIONS, 8, Mar. 2017, [Peer-reviewed]
English, Scientific journal

Engineering an Obligate Photoautotrophic Cyanobacterium to Utilize Glycerol for Growth and Chemical Production
Masahiro Kanno, Shota Atsumi
ACS SYNTHETIC BIOLOGY, 6, 1, 69, 75, Jan. 2017, [Peer-reviewed]
English, Scientific journal

Cyanobacterial metabolic engineering for biofuel and chemical production
Neal J. Oliver, Christine A. Rabinovitch-Deere, Austin L. Carroll, Nicole E. Nozzi, Anna E. Case, Shota Atsumi
CURRENT OPINION IN CHEMICAL BIOLOGY, 35, 43, 50, Dec. 2016, [Peer-reviewed]
English

Biological conversion of gaseous alkenes to liquid chemicals
Shuchi H. Desai, Irina Koryakina, Anna E. Case, Michael D. Toney, Shota Atsumi
METABOLIC ENGINEERING, 38, 98, 104, Nov. 2016, [Peer-reviewed]
English, Scientific journal

Cyanobacterial chemical production
Anna E. Case, Shota Atsumi
JOURNAL OF BIOTECHNOLOGY, 231, 106, 114, Aug. 2016, [Peer-reviewed]
English

2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption
Jordan T. McEwen, Masahiro Kanno, Shota Atsumi
METABOLIC ENGINEERING, 36, 28, 36, Jul. 2016, [Peer-reviewed]
English, Scientific journal

Microbial production of scent and flavor compounds
Austin L. Carroll, Shuchi H. Desai, Shota Atsumi
CURRENT OPINION IN BIOTECHNOLOGY, 37, 8, 15, Feb. 2016, [Peer-reviewed]
English

Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria
Nicole E. Nozzi, Shota Atsumi
ACS SYNTHETIC BIOLOGY, 4, 11, 1197, 1204, Nov. 2015, [Peer-reviewed]
English, Scientific journal

Two-dimensional isobutyl acetate production pathways to improve carbon yield
Yohei Tashiro, Shuchi H. Desai, Shota Atsumi
NATURE COMMUNICATIONS, 6, Jun. 2015, [Peer-reviewed]
English, Scientific journal

A carbon sink pathway increases carbon productivity in cyanobacteria
John W. K. Oliver, Shota Atsumi
METABOLIC ENGINEERING, 29, 106, 112, May 2015, [Peer-reviewed]
English, Scientific journal

Isobutanol production from cellobionic acid in Escherichia coli
Shuchi H. Desai, Christine A. Rabinovitch-Deere, Zhiliang Fan, Shota Atsumi
MICROBIAL CELL FACTORIES, 14, Apr. 2015, [Peer-reviewed]
English, Scientific journal

2-Keto acids based biosynthesis pathways for renewable fuels and chemicals
Yohei Tashiro, Gabriel M. Rodriguez, Shota Atsumi
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 42, 3, 361, 373, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Metabolic engineering for higher alcohol production
Nicole E. Nozzi, Shuchi H. Desai, Annae E. Case, Shota Atsumi
METABOLIC ENGINEERING, 25, 174, 182, Sep. 2014, [Peer-reviewed]
English

Toward aldehyde and alkane production by removing aldehyde reductase activity in Escherichia coli
Gabriel M. Rodriguez, Shota Atsumi
METABOLIC ENGINEERING, 25, 227, 237, Sep. 2014, [Peer-reviewed]
English, Scientific journal

Metabolic design for cyanobacterial chemical synthesis
John W. K. Oliver, Shota Atsumi
PHOTOSYNTHESIS RESEARCH, 120, 3, 249, 261, Jun. 2014, [Peer-reviewed]
English

Expanding ester biosynthesis in Escherichia coli
Gabriel M. Rodriguez, Yohei Tashiro, Shota Atsumi
NATURE CHEMICAL BIOLOGY, 10, 4, 259, +, Apr. 2014, [Peer-reviewed]
English, Scientific journal

Isobutanol production from cellobiose in Escherichia coli
Shuchi H. Desai, Christine A. Rabinovitch-Deere, Yohei Tashiro, Shota Atsumi
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98, 8, 3727, 3736, Apr. 2014, [Peer-reviewed]
English, Scientific journal

Combinatorial optimization of cyanobacterial 2,3-butanediol production
John W. K. Oliver, Iara M. P. Machado, Hisanari Yoneda, Shota Atsumi
METABOLIC ENGINEERING, 22, 76, 82, Mar. 2014, [Peer-reviewed]
English, Scientific journal

Biological Production of 2-Butanone in Escherichia coli
Hisanari Yoneda, Dean J. Tantillo, Shota Atsumi
CHEMSUSCHEM, 7, 1, 92, 95, Jan. 2014, [Peer-reviewed]
English, Scientific journal

Photosynthetic approaches to chemical biotechnology
Shuchi H. Desai, Shota Atsumi
CURRENT OPINION IN BIOTECHNOLOGY, 24, 6, 1031, 1036, Dec. 2013, [Peer-reviewed]
English

Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light
Tamami Kusakabe, Tsuneyuki Tatsuke, Keigo Tsuruno, Yasutaka Hirokawa, Shota Atsumi, James C. Liao, Taizo Hanai
METABOLIC ENGINEERING, 20, 101, 108, Nov. 2013, [Peer-reviewed]
English, Scientific journal

Synthetic Biology and Metabolic Engineering Approaches To Produce Biofuels
Rabinovitch-Deere Christine A, Oliver John W. K, Rodriguez Gabriel M, Atsumi Shota
CHEMICAL REVIEWS, 113, 7, 4611, 4632, Jul. 2013, [Peer-reviewed]

Engineering Synechococcus elongatus PCC 7942 for Continuous Growth under Diurnal Conditions
McEwen Jordan T, Machado Iara M. P, Connor Michael R, Atsumi Shota
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79, 5, 1668, 1675, Mar. 2013, [Peer-reviewed]

Cyanobacterial conversion of carbon dioxide to 2,3-butanediol
John W. K. Oliver, Iara M. P. Machado, Hisanari Yoneda, Shota Atsumi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110, 4, 1249, 1254, Jan. 2013, [Peer-reviewed]
English, Scientific journal

Cyanobacterial biofuel production
Iara M. P. Machado, Shota Atsumi
JOURNAL OF BIOTECHNOLOGY, 162, 1, 50, 56, Nov. 2012, [Peer-reviewed]
English, Scientific journal

Alternative biofuel production in non-natural hosts
Jordan T. McEwen, Shota Atsumi
CURRENT OPINION IN BIOTECHNOLOGY, 23, 5, 744, 750, Oct. 2012, [Peer-reviewed]
English

Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity
Gabriel M. Rodriguez, Shota Atsumi
MICROBIAL CELL FACTORIES, 11, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Recent progress in synthetic biology for microbial production of C3-C10 alcohols
Edna N. Lamsen, Shota Atsumi
FRONTIERS IN MICROBIOLOGY, 3, 2012, [Peer-reviewed]
English

Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli
Shota Atsumi, Tung-Yun Wu, Iara M. P. Machado, Wei-Chih Huang, Pao-Yang Chen, Matteo Pellegrini, James C. Liao
MOLECULAR SYSTEMS BIOLOGY, 6, Dec. 2010, [Peer-reviewed]
English, Scientific journal

Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes
Shota Atsumi, Tung-Yun Wu, Eva-Maria Eckl, Sarah D. Hawkins, Thomas Buelter, James C. Liao
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85, 3, 651, 657, Jan. 2010, [Peer-reviewed]
English, Scientific journal

An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study
Ieong Wong, Shota Atsumi, Wei-Chih Huang, Tung-Yun Wu, Taizo Hanai, Miu-Ling Lam, Ping Tang, Jian Yang, James C. Liao, Chih-Ming Ho
LAB ON A CHIP, 10, 20, 2710, 2719, 2010, [Peer-reviewed]
English, Scientific journal

Synthetic Biology Guides Biofuel Production
Michael R. Connor, Shota Atsumi
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2010, [Peer-reviewed]
English

Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
Shota Atsumi, Wendy Higashide, James C. Liao
NATURE BIOTECHNOLOGY, 27, 12, 1177, U142, Dec. 2009, [Peer-reviewed]
English, Scientific journal

Acetolactate Synthase from Bacillus subtilis Serves as a 2-Ketoisovalerate Decarboxylase for Isobutanol Biosynthesis in Escherichia coli
Shota Atsumi, Zhen Li, James C. Liao
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75, 19, 6306, 6311, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli
Shota Atsumi, James C. Liao
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74, 24, 7802, 7808, Dec. 2008, [Peer-reviewed]
English, Scientific journal

Metabolic engineering of Escherichia coli for 1-butanol production
Shota Atsumi, Anthony F. Cann, Michael R. Connor, Claire R. Shen, Kevin M. Smith, Mark P. Brynildsen, Katherine J. Y. Chou, Taizo Hanai, James C. Liao
METABOLIC ENGINEERING, 10, 6, 305, 311, Nov. 2008, [Peer-reviewed]
English, Scientific journal

Metabolic engineering for advanced biofuels production from Escherichia coli
Shota Atsumi, James C. Liao
CURRENT OPINION IN BIOTECHNOLOGY, 19, 5, 414, 419, Oct. 2008, [Peer-reviewed]
English

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Shota Atsumi, Taizo Hanai, James C. Liao
NATURE, 451, 7174, 86, U13, Jan. 2008, [Peer-reviewed]
English, Scientific journal

A synthetic phage lambda regulatory circuit
Shota Atsumi, John W. Little
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103, 50, 19045, 19050, Dec. 2006, [Peer-reviewed]
English, Scientific journal

Role of the lytic repressor in prophage induction of phage lambda as analyzed by a module-replacement approach
S Atsumi, JW Little
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103, 12, 4558, 4563, Mar. 2006, [Peer-reviewed]
English, Scientific journal

Regulatory circuit design and evolution using phage lambda
S Atsumi, JW Little
GENES & DEVELOPMENT, 18, 17, 2086, 2094, Sep. 2004, [Peer-reviewed]
English, Scientific journal

Putative intermediary stages for the molecular evolution from a ribozyme to a catalytic RNP
Y Ikawa, K Tsuda, S Matsumura, S Atsumi, T Inoue
NUCLEIC ACIDS RESEARCH, 31, 5, 1488, 1496, Mar. 2003, [Peer-reviewed]
English, Scientific journal

Selections for constituting new RNA-protein interactions in catalytic RNP
S Atsumi, Y Ikawa, H Shiraishi, T Inoue
NUCLEIC ACIDS RESEARCH, 31, 2, 661, 669, Jan. 2003, [Peer-reviewed]
English, Scientific journal

Modeling of a possible evolutional process from a ribozyme to a catalytic RNP.
Ikawa Y, Tsuda K, Matsumura S, Atsumi S, Inoue T
Nucleic acids research. Supplement (2001), 2, 119, 120, 2002, [Peer-reviewed]

A comparative study on two GNRA-tetraloop receptors: 11-nt and IC3 motifs.
Ikawa Y, Nohmi K, Atsumi S, Shiraishi H, Inoue T, Jou
J Biochem (Tokyo), 130, 2, 251, 255, The Japanese Biochemical Society, Aug. 2001, [Peer-reviewed]
English, Ikawa Y, Nohmi K, Atsumi S, Shiraishi H, Inoue T, Journal of biochemistry, 2001, vol. 130, no. 2, pp. 251-255

Design and development of a catalytic ribonucleoprotein
Atsumi S, Ikawa Y, Shiraishi H, Inoue T
EMBO Journal, 20, 19, 5453, 5460, 2001, [Peer-reviewed]

2-Keto acids based biosynthesis pathways for renewable fuels and chemicals
Yohei Tashiro, Shuchi Desai, Gabriel Rodriguez, Shota Atsumi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 249, Mar. 2015
English, Summary international conference

Cyanobacterial conversion of carbon dioxide to 2,3-butanediol
Shota Atsumi, John W. K. Oliver, Iara M. P. Machado, Hisanari Yoneda, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 247, Mar. 2014
English, Summary international conference

Systematic construction of biosynthetic pathways for fuel molecules
Gabriel Rodriguez, Yohei Tashiro, Shota Atsumi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 247, Mar. 2014
English, Summary international conference

生物生産へ向けた代謝経路設計(バイオミディア)
田代 洋平, 渥美 正太, 生物工学会誌 : seibutsu-kogaku kaishi, 92, 10, 562, 562, 2014
日本生物工学会, Japanese

Isobutyraldehyde production in Escherichia coli by removing aldehyde reductase activity
Gabriel M. Rodriguez, Shota Atsumi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 245, Apr. 2013
English, Summary international conference

Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways
Claire R. Shen, James C. Liao, Shota Atsumi, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 239, Mar. 2010
English, Summary international conference

BIOT 241-Engineering synthetic pathways for production of higher alcohols as biofuels
Shota Atsumi, James C. Liao, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 236, Aug. 2008
English, Summary international conference

BIOT 146-Engineering synthetic pathways for production of butanol as biofuels
Shota Atsumi, James C. Liao, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 234, Aug. 2007
English, Summary international conference

Engineered synthetic pathway for isopropanol production in Escherichia coli
HANAI T., Appl. Environ. Microbiol., 73, 24, 7814, 7818, 2007

Unexpected Transformation of E. coli caused by Tetrahymena ribozyme
K. Kazue, ATSUMI S., IKAWA Y., SHIRAISI H., INOUE T., 日本分子生物学会年会プログラム・講演要旨集, 21, 453, 453, 01 Dec. 1998
Japanese