ATSUMI　SHOTA (アツミ　シヨウタ)

農学研究院　食水土資源グローバルセンター教授
Last Updated :2026/04/14

■研究者基本情報

学位

  • 理学博士, 京都大学, 2002年03月

Researchmap個人ページ

ホームページURL

J-Global ID

研究分野

  • ライフサイエンス, 応用微生物学

■経歴

学歴

  • 2002年03月, 京都大学, 理学研究科, 日本国

■研究活動情報

論文

  • Microbial Production of Human Milk Oligosaccharides.
    Dileep Sai Kumar Palur, Shannon R Pressley, Shota Atsumi
    Molecules (Basel, Switzerland), 28, 3, 2023年02月03日, ［査読有り］, ［招待有り］, ［最終著者, 責任著者］, ［国際誌］
    英語, 研究論文（学術雑誌）, Human milk oligosaccharides (HMOs) are complex nonnutritive sugars present in human milk. These sugars possess prebiotic, immunomodulatory, and antagonistic properties towards pathogens and therefore are important for the health and well-being of newborn babies. Lower prevalence of breastfeeding around the globe, rising popularity of nutraceuticals, and low availability of HMOs have inspired efforts to develop economically feasible and efficient industrial-scale production platforms for HMOs. Recent progress in synthetic biology and metabolic engineering tools has enabled microbial systems to be a production system of HMOs. In this regard, the model organism Escherichia coli has emerged as the preferred production platform. Herein, we summarize the remarkable progress in the microbial production of HMOs and discuss the challenges and future opportunities in unraveling the scope of production of complex HMOs. We focus on the microbial production of five HMOs that have been approved for their commercialization.
  • Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria.
    Shunichi Kobayashi, Shota Atsumi, Kazunori Ikebukuro, Koji Sode, Ryutaro Asano
    Microbial cell factories, 21, 1, 7, 7, 2022年01月06日, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, BACKGROUND: Cyanobacteria are engineered via heterologous biosynthetic pathways to produce value-added chemicals via photosynthesis. Various chemicals have been successfully produced in engineered cyanobacteria. Chemical inducer-dependent promoters are used to induce the expression of target biosynthetic pathway genes. A chemical inducer is not ideal for large-scale reactions owing to its high cost; therefore, it is important to develop scaling-up methods to avoid their use. In this study, we designed a green light-inducible alcohol production system using the CcaS/CcaR green light gene expression system in the cyanobacterium Synechocystis sp. PCC 6803 (PCC 6803). RESULTS: To establish the green light-inducible production of isobutanol and 3-methyl-1-butanol (3MB) in PCC 6803, keto-acid decarboxylase (kdc) and alcohol dehydrogenase (adh) were expressed under the control of the CcaS/CcaR system. Increases in the transcription level were induced by irradiation with red and green light without severe effects on host cell growth. We found that the production of isobutanol and 3MB from carbon dioxide (CO2) was induced under red and green light illumination and was substantially repressed under red light illumination alone. Finally, production titers of isobutanol and 3MB reached 238 mg L-1 and 75 mg L-1, respectively, in 5 days under red and green light illumination, and these values are comparable to those reported in previous studies using chemical inducers. CONCLUSION: A green light-induced alcohol production system was successfully integrated into cyanobacteria to produce value-added chemicals without using expensive chemical inducers. The green light-regulated production of isobutanol and 3MB from CO2 is eco-friendly and cost-effective. This study demonstrates that light regulation is a potential tool for producing chemicals and increases the feasibility of cyanobacterial bioprocesses.
  • Adaptive laboratory evolution for improved tolerance of isobutyl acetate in Escherichia coli.
    Morgan M Matson, Mateo M Cepeda, Angela Zhang, Anna E Case, Erol S Kavvas, Xiaokang Wang, Austin L Carroll, Ilias Tagkopoulos, Shota Atsumi
    Metabolic engineering, 69, 50, 58, 2022年01月, ［査読有り］, ［最終著者, 責任著者］, ［国際誌］
    英語, 研究論文（学術雑誌）, Previously, Escherichia coli was engineered to produce isobutyl acetate (IBA). Titers greater than the toxicity threshold (3 g/L) were achieved by using layer-assisted production. To avoid this costly and complex method, adaptive laboratory evolution (ALE) was applied to E. coli for improved IBA tolerance. Over 37 rounds of selective pressure, 22 IBA-tolerant mutants were isolated. Remarkably, these mutants not only tolerate high IBA concentrations, they also produce higher IBA titers. Using whole-genome sequencing followed by CRISPR/Cas9 mediated genome editing, the mutations (SNPs in metH, rho and deletion of arcA) that confer improved tolerance and higher titers were elucidated. The improved IBA titers in the evolved mutants were a result of an increased supply of acetyl-CoA and altered transcriptional machinery. Without the use of phase separation, a strain capable of 3.2-fold greater IBA production than the parent strain was constructed by combing select beneficial mutations. These results highlight the impact improved tolerance has on the production capability of a biosynthetic system.
  • Synthetic Biology Approaches for Improving Chemical Production in Cyanobacteria.
    Tanner R Treece, Jake N Gonzales, Joseph R Pressley, Shota Atsumi
    Frontiers in bioengineering and biotechnology, 10, 869195, 869195, 2022年, ［査読有り］, ［招待有り］, ［最終著者, 責任著者］, ［国際誌］
    英語, 研究論文（学術雑誌）, Biological chemical production has gained traction in recent years as a promising renewable alternative to traditional petrochemical based synthesis. Of particular interest in the field of metabolic engineering are photosynthetic microorganisms capable of sequestering atmospheric carbon dioxide. CO2 levels have continued to rise at alarming rates leading to an increasingly uncertain climate. CO2 can be sequestered by engineered photosynthetic microorganisms and used for chemical production, representing a renewable production method for valuable chemical commodities such as biofuels, plastics, and food additives. The main challenges in using photosynthetic microorganisms for chemical production stem from the seemingly inherent limitations of carbon fixation and photosynthesis resulting in slower growth and lower average product titers compared to heterotrophic organisms. Recently, there has been an increase in research around improving photosynthetic microorganisms as renewable chemical production hosts. This review will discuss the various efforts to overcome the intrinsic inefficiencies of carbon fixation and photosynthesis, including rewiring carbon fixation and photosynthesis, investigating alternative carbon fixation pathways, installing sugar catabolism to supplement carbon fixation, investigating newly discovered fast growing photosynthetic species, and using new synthetic biology tools such as CRISPR to radically alter metabolism.
  • Microbial production of human milk oligosaccharide lactodifucotetraose.
    Angela Zhang, Lei Sun, Yuanyuan Bai, Hai Yu, John B McArthur, Xi Chen, Shota Atsumi
    Metabolic engineering, 66, 12, 20, 2021年07月, ［査読有り］, ［最終著者, 責任著者］, ［国際誌］
    英語, 研究論文（学術雑誌）, Human milk oligosaccharides (HMOs) are potent bioactive compounds that modulate neonatal health and are of interest for development as potential drug treatments for adult diseases. The potential of these molecules, their limited access from natural sources, and difficulty in large-scale isolation of individual HMOs for studies and applications have motivated the development of chemical syntheses and in vitro enzymatic catalysis strategies. Whole cell biocatalysts are emerging as alternative self-regulating production platforms that have the potential to reduce the cost for enzymatic synthesis of HMOs. Whole cell biocatalysts for the production of short-chained, linear and small monofucosylated HMOs have been reported but those for fucosylated structures with higher complexity have not been explored. In this study, we established a strategy for producing a difucosylated HMO, lactodifucotetraose (LDFT), from lactose and L-fucose in Escherichia coli. We used two bacterial fucosyltransferases with narrow acceptor selectivity to drive the sequential fucosylation of lactose and intermediate 2'-fucosyllactose (2'-FL) to produce LDFT. Deletion of substrate degradation pathways that decoupled cellular growth from LDFT production, enhanced expression of native substrate transporters and modular induction of the genes in the LDFT biosynthetic pathway allowed complete conversion of lactose into LDFT and minor quantities of the side product 3-fucosyllactose (3-FL). Overall, 5.1 g/L of LDFT was produced from 3 g/L lactose and 3 g/L L-fucose in 24 h. Our results demonstrate promising applications of engineered microbial biosystems for the production of multi-fucosylated HMOs for biochemical studies.
  • Nonphotosynthetic Biological CO2 Reduction.
    Jake N Gonzales, Morgan M Matson, Shota Atsumi
    Biochemistry, 58, 11, 1470, 1477, 2019年03月19日, ［査読有り］, ［招待有り］, ［最終著者］, ［国際誌］
    英語, 研究論文（学術雑誌）, Alarming changes in environmental conditions have prompted significant research into producing renewable commodities from sources other than fossil fuels. One such alternative is CO2, a determinate greenhouse gas with historically high atmospheric levels. If sequestered, CO2 could be used as a highly renewable feedstock for industrially relevant products and fuels. The vast majority of atmospheric CO2 fixation is accomplished by photosynthetic organisms, which have unfortunately proven difficult to utilize as chassis for industrial production. Nonphotosynthetic CO2 fixing microorganisms and pathways have recently attracted scientific and commercial interest. This Perspective will review promising alternate CO2 fixation strategies and their potential to supply microbially produced fuels and commodity chemicals, such as higher alcohols. Acetogenic fermentation and microbial electrosynthesis are the primary focuses of this review.
  • Metabolic engineering tools in model cyanobacteria.
    Austin L Carroll, Anna E Case, Angela Zhang, Shota Atsumi
    Metabolic engineering, 50, 47, 56, 2018年11月, ［査読有り］, ［招待有り］, ［最終著者, 責任著者］, ［国際誌］
    英語, 研究論文（学術雑誌）, Developing sustainable routes for producing chemicals and fuels is one of the most important challenges in metabolic engineering. Photoautotrophic hosts are particularly attractive because of their potential to utilize light as an energy source and CO2 as a carbon substrate through photosynthesis. Cyanobacteria are unicellular organisms capable of photosynthesis and CO2 fixation. While engineering in heterotrophs, such as Escherichia coli, has result in a plethora of tools for strain development and hosts capable of producing valuable chemicals efficiently, these techniques are not always directly transferable to cyanobacteria. However, recent efforts have led to an increase in the scope and scale of chemicals that cyanobacteria can produce. Adaptations of important metabolic engineering tools have also been optimized to function in photoautotrophic hosts, which include Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9, 13C Metabolic Flux Analysis (MFA), and Genome-Scale Modeling (GSM). This review explores innovations in cyanobacterial metabolic engineering, and highlights how photoautotrophic metabolism has shaped their development.
  • Electrical-biological hybrid system for CO2 reduction
    Yohei Tashiro, Shinichi Hirano, Morgan M. Matson, Shota Atsumi, Akihiko Kondo
    Metabolic Engineering, 47, 211, 218, 2018年05月01日, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Photomixotrophic chemical production in cyanobacteria
    Matson Morgan M, Atsumi Shota
    CURRENT OPINION IN BIOTECHNOLOGY, 50, 65, 71, 2018年04月, ［査読有り］
  • Systematic Approaches to Efficiently Produce 2,3-Butanediol in a Marine Cyanobacterium
    Nicole E. Nozzi, Anna E. Case, Austin L. Carroll, Shota Atsumi
    ACS SYNTHETIC BIOLOGY, 6, 11, 2136, 2144, 2017年11月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Carbon recycling by cyanobacteria: improving CO2 fixation through chemical production
    Angela Zhang, Austin L. Carroll, Shota Atsumi
    FEMS MICROBIOLOGY LETTERS, 364, 16, 2017年08月, ［査読有り］
    英語
  • Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria
    Masahiro Kanno, Austin L. Carroll, Shota Atsumi
    NATURE COMMUNICATIONS, 8, 2017年03月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Engineering an Obligate Photoautotrophic Cyanobacterium to Utilize Glycerol for Growth and Chemical Production
    Masahiro Kanno, Shota Atsumi
    ACS SYNTHETIC BIOLOGY, 6, 1, 69, 75, 2017年01月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Cyanobacterial metabolic engineering for biofuel and chemical production
    Neal J. Oliver, Christine A. Rabinovitch-Deere, Austin L. Carroll, Nicole E. Nozzi, Anna E. Case, Shota Atsumi
    CURRENT OPINION IN CHEMICAL BIOLOGY, 35, 43, 50, 2016年12月, ［査読有り］
    英語
  • Biological conversion of gaseous alkenes to liquid chemicals
    Shuchi H. Desai, Irina Koryakina, Anna E. Case, Michael D. Toney, Shota Atsumi
    METABOLIC ENGINEERING, 38, 98, 104, 2016年11月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Cyanobacterial chemical production
    Anna E. Case, Shota Atsumi
    JOURNAL OF BIOTECHNOLOGY, 231, 106, 114, 2016年08月, ［査読有り］
    英語
  • 2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption
    Jordan T. McEwen, Masahiro Kanno, Shota Atsumi
    METABOLIC ENGINEERING, 36, 28, 36, 2016年07月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Microbial production of scent and flavor compounds
    Austin L. Carroll, Shuchi H. Desai, Shota Atsumi
    CURRENT OPINION IN BIOTECHNOLOGY, 37, 8, 15, 2016年02月, ［査読有り］
    英語
  • Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria
    Nicole E. Nozzi, Shota Atsumi
    ACS SYNTHETIC BIOLOGY, 4, 11, 1197, 1204, 2015年11月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Two-dimensional isobutyl acetate production pathways to improve carbon yield
    Yohei Tashiro, Shuchi H. Desai, Shota Atsumi
    NATURE COMMUNICATIONS, 6, 2015年06月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • A carbon sink pathway increases carbon productivity in cyanobacteria
    John W. K. Oliver, Shota Atsumi
    METABOLIC ENGINEERING, 29, 106, 112, 2015年05月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Isobutanol production from cellobionic acid in Escherichia coli
    Shuchi H. Desai, Christine A. Rabinovitch-Deere, Zhiliang Fan, Shota Atsumi
    MICROBIAL CELL FACTORIES, 14, 2015年04月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • 2-Keto acids based biosynthesis pathways for renewable fuels and chemicals
    Yohei Tashiro, Gabriel M. Rodriguez, Shota Atsumi
    JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 42, 3, 361, 373, 2015年03月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Metabolic engineering for higher alcohol production
    Nicole E. Nozzi, Shuchi H. Desai, Annae E. Case, Shota Atsumi
    METABOLIC ENGINEERING, 25, 174, 182, 2014年09月, ［査読有り］
    英語
  • Toward aldehyde and alkane production by removing aldehyde reductase activity in Escherichia coli
    Gabriel M. Rodriguez, Shota Atsumi
    METABOLIC ENGINEERING, 25, 227, 237, 2014年09月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Metabolic design for cyanobacterial chemical synthesis
    John W. K. Oliver, Shota Atsumi
    PHOTOSYNTHESIS RESEARCH, 120, 3, 249, 261, 2014年06月, ［査読有り］
    英語
  • Expanding ester biosynthesis in Escherichia coli
    Gabriel M. Rodriguez, Yohei Tashiro, Shota Atsumi
    NATURE CHEMICAL BIOLOGY, 10, 4, 259, +, 2014年04月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Isobutanol production from cellobiose in Escherichia coli
    Shuchi H. Desai, Christine A. Rabinovitch-Deere, Yohei Tashiro, Shota Atsumi
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98, 8, 3727, 3736, 2014年04月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Combinatorial optimization of cyanobacterial 2,3-butanediol production
    John W. K. Oliver, Iara M. P. Machado, Hisanari Yoneda, Shota Atsumi
    METABOLIC ENGINEERING, 22, 76, 82, 2014年03月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Biological Production of 2-Butanone in Escherichia coli
    Hisanari Yoneda, Dean J. Tantillo, Shota Atsumi
    CHEMSUSCHEM, 7, 1, 92, 95, 2014年01月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Photosynthetic approaches to chemical biotechnology
    Shuchi H. Desai, Shota Atsumi
    CURRENT OPINION IN BIOTECHNOLOGY, 24, 6, 1031, 1036, 2013年12月, ［査読有り］
    英語
  • Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light
    Tamami Kusakabe, Tsuneyuki Tatsuke, Keigo Tsuruno, Yasutaka Hirokawa, Shota Atsumi, James C. Liao, Taizo Hanai
    METABOLIC ENGINEERING, 20, 101, 108, 2013年11月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Synthetic Biology and Metabolic Engineering Approaches To Produce Biofuels
    Rabinovitch-Deere Christine A, Oliver John W. K, Rodriguez Gabriel M, Atsumi Shota
    CHEMICAL REVIEWS, 113, 7, 4611, 4632, 2013年07月, ［査読有り］
  • Engineering Synechococcus elongatus PCC 7942 for Continuous Growth under Diurnal Conditions
    McEwen Jordan T, Machado Iara M. P, Connor Michael R, Atsumi Shota
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79, 5, 1668, 1675, 2013年03月, ［査読有り］
  • Cyanobacterial conversion of carbon dioxide to 2,3-butanediol
    John W. K. Oliver, Iara M. P. Machado, Hisanari Yoneda, Shota Atsumi
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110, 4, 1249, 1254, 2013年01月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Cyanobacterial biofuel production
    Iara M. P. Machado, Shota Atsumi
    JOURNAL OF BIOTECHNOLOGY, 162, 1, 50, 56, 2012年11月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Alternative biofuel production in non-natural hosts
    Jordan T. McEwen, Shota Atsumi
    CURRENT OPINION IN BIOTECHNOLOGY, 23, 5, 744, 750, 2012年10月, ［査読有り］
    英語
  • Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity
    Gabriel M. Rodriguez, Shota Atsumi
    MICROBIAL CELL FACTORIES, 11, 2012年06月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Recent progress in synthetic biology for microbial production of C3-C10 alcohols
    Edna N. Lamsen, Shota Atsumi
    FRONTIERS IN MICROBIOLOGY, 3, 2012年, ［査読有り］
    英語
  • Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli
    Shota Atsumi, Tung-Yun Wu, Iara M. P. Machado, Wei-Chih Huang, Pao-Yang Chen, Matteo Pellegrini, James C. Liao
    MOLECULAR SYSTEMS BIOLOGY, 6, 2010年12月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes
    Shota Atsumi, Tung-Yun Wu, Eva-Maria Eckl, Sarah D. Hawkins, Thomas Buelter, James C. Liao
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85, 3, 651, 657, 2010年01月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study
    Ieong Wong, Shota Atsumi, Wei-Chih Huang, Tung-Yun Wu, Taizo Hanai, Miu-Ling Lam, Ping Tang, Jian Yang, James C. Liao, Chih-Ming Ho
    LAB ON A CHIP, 10, 20, 2710, 2719, 2010年, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Synthetic Biology Guides Biofuel Production
    Michael R. Connor, Shota Atsumi
    JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2010年, ［査読有り］
    英語
  • Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
    Shota Atsumi, Wendy Higashide, James C. Liao
    NATURE BIOTECHNOLOGY, 27, 12, 1177, U142, 2009年12月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Acetolactate Synthase from Bacillus subtilis Serves as a 2-Ketoisovalerate Decarboxylase for Isobutanol Biosynthesis in Escherichia coli
    Shota Atsumi, Zhen Li, James C. Liao
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75, 19, 6306, 6311, 2009年10月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli
    Shota Atsumi, James C. Liao
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74, 24, 7802, 7808, 2008年12月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Metabolic engineering of Escherichia coli for 1-butanol production
    Shota Atsumi, Anthony F. Cann, Michael R. Connor, Claire R. Shen, Kevin M. Smith, Mark P. Brynildsen, Katherine J. Y. Chou, Taizo Hanai, James C. Liao
    METABOLIC ENGINEERING, 10, 6, 305, 311, 2008年11月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Metabolic engineering for advanced biofuels production from Escherichia coli
    Shota Atsumi, James C. Liao
    CURRENT OPINION IN BIOTECHNOLOGY, 19, 5, 414, 419, 2008年10月, ［査読有り］
    英語
  • Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
    Shota Atsumi, Taizo Hanai, James C. Liao
    NATURE, 451, 7174, 86, U13, 2008年01月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • A synthetic phage lambda regulatory circuit
    Shota Atsumi, John W. Little
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103, 50, 19045, 19050, 2006年12月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Role of the lytic repressor in prophage induction of phage lambda as analyzed by a module-replacement approach
    S Atsumi, JW Little
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103, 12, 4558, 4563, 2006年03月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Regulatory circuit design and evolution using phage lambda
    S Atsumi, JW Little
    GENES & DEVELOPMENT, 18, 17, 2086, 2094, 2004年09月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Putative intermediary stages for the molecular evolution from a ribozyme to a catalytic RNP
    Y Ikawa, K Tsuda, S Matsumura, S Atsumi, T Inoue
    NUCLEIC ACIDS RESEARCH, 31, 5, 1488, 1496, 2003年03月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Selections for constituting new RNA-protein interactions in catalytic RNP
    S Atsumi, Y Ikawa, H Shiraishi, T Inoue
    NUCLEIC ACIDS RESEARCH, 31, 2, 661, 669, 2003年01月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Modeling of a possible evolutional process from a ribozyme to a catalytic RNP.
    Ikawa Y, Tsuda K, Matsumura S, Atsumi S, Inoue T
    Nucleic acids research. Supplement (2001), 2, 119, 120, 2002年, ［査読有り］
  • A comparative study on two GNRA-tetraloop receptors: 11-nt and IC3 motifs.
    Ikawa Y, Nohmi K, Atsumi S, Shiraishi H, Inoue T, Jou
    The Journal of Biochemistry, 130, 2, 251, 255, The Japanese Biochemical Society, 2001年08月, ［査読有り］
    英語, Ikawa Y, Nohmi K, Atsumi S, Shiraishi H, Inoue T, Journal of biochemistry, 2001, vol. 130, no. 2, pp. 251-255
  • Design and development of a catalytic ribonucleoprotein
    Atsumi S, Ikawa Y, Shiraishi H, Inoue T
    EMBO Journal, 20, 19, 5453, 5460, 2001年, ［査読有り］

その他活動・業績

共同研究・競争的資金等の研究課題

  • 藻類バイオマスから化成品を生産する一貫バイオプロセスの構築
    科学研究費助成事業
    2018年04月01日 - 2023年03月31日
    渥美 正太, 堀 千明, 高須賀 太一
    本研究では、褐藻バイオマスを利用したバイオ化成品生産のための大腸菌作出を行っている。2020年度は、大腸菌TA1021株のicd遺伝子欠損株の作成および1,4-butanediol生産のための３種類の遺伝子、DEHU reductase (dehR)、5-keto-4-deoxy-D-glucarate dehydratase (KdgD)、および2-ketoglutarate semialdehyde dehydrogenase (XylA)の３種類を保持するプラスミド（Midium copy plasmid）の作成を行った。ICD遺伝子欠損株については当初ゲノム編集技術によりICD遺伝子を欠損させる予定であったが、欠損を行うために設計した相同配列の長さが短かったため再度設計し、欠損を試みた。同時にP1ファージを用いた transductionを、DNA供与株JW1122（icd欠損）を用いて行ったところ、目的のicd遺伝子欠損株が作成できた。本欠損株については、シークエンスで確認予定である。プラスミド作成については、3種類のdehRと3種類のkdgD遺伝子および1種類のXylA遺伝子をタンデムにつないだプラスミド計9種の作成を行い、完成した。
    褐藻バイオマスに含まれるアルギン酸を構成する単糖であるDEHUを大腸菌細胞内に取り込むための輸送タンパク質をコードした遺伝子3種類については、クローニング（こちらはLow copy plasmidを使用）を完了している。
    日本学術振興会, 基盤研究(B), 北海道大学, 18H02011