Mar. 2010, 日本水産学会, 水産学奨励賞　　　　　　　　　　　　　　　
魚類の卵形成に関与する卵黄前駆物質および その受容体に関する研究
平松 尚志

Establishment of an all-female monosex population in black rockfish (Sebastes schlegelii) through an indirect feminization method
Mutea Fridah Gacheri, Yo Yamaguchi, Takuma Kawasaki, Takashi Todo, Naoshi Hiramatsu
Aquaculture, 609, 742818, 742818, Elsevier BV, Oct. 2025
Scientific journal

Sebastexins: Novel members of the three-finger protein family found in urine of viviparous Sebastes rockfish.
Yo Yamaguchi, Jin Namgung, Takafumi Amagai, Kiyoshi Soyano, Takuma Kawasaki, Takashi Todo, Naoshi Hiramatsu
Biochemical and biophysical research communications, 784, 152669, 152669, 17 Sep. 2025, [International Magazine]
English, Scientific journal, Viviparous Sebastes rockfish males place their urogenital papilla close to the females' snout during courtship behavior, possibly to expose females to certain urinary substances. The urine of rockfish males contains abundant protein constituents that putatively act in inter-sexual chemical signaling. Here, we characterized low-molecular-weight urinary proteins (LMWups) from black rockfish (S. schlegelii). The LMWups were semi-purified from the urine of a male by cation-exchange chromatography for N-terminal sequencing, yielding a sequence of 35 amino acids (AAs) with only five unidentified residues. The black rockfish genome harbored three regions whose deduced AAs matched the obtained N-terminal sequence. Mapping of public RNA-seq reads enabled us to infer gene structures underlying these genomic regions. The proteins coded by these regions showed characteristics of members of the three-finger protein (TFP) family, which includes snake three-finger toxins and amphibian pheromones. We termed them as "sebastexins". Further in silico searches identified seven additional potential sebastexin gene loci in the black rockfish genome. Transcriptome analysis using a public dataset suggested that the kidney and liver are the major tissues expressing the sebastexin transcripts. The presence of sebastexin transcripts and proteins in the kidney and urine of males were empirically verified by cDNA cloning, Western blotting, and mass spectrometry. Histological analysis showed that a major sebastexin isoform is, at least in part, produced in the epithelial cells of the renal tubules of the male kidney. Collectively, these findings identify and partly characterize sebastexin TFP family proteins in rockfish urine. The potential biological function of these sebastexins is discussed.

Androgens induce renal synthesis of urinary lipocalin-family protein, a potential inter-sexual transmitter in viviparous rockfish
Yo Yamaguchi, Jun Nagata, Takuma Kawasaki, Takashi Todo, Naoshi Hiramatsu
Biochimica et Biophysica Acta (BBA) - General Subjects, 1869, 3, 130756, 130756, Elsevier BV, Mar. 2025
Scientific journal

Serum lipocalin-like protein in Sebastes rockfish: A novel biomarker for reproductive stage males
Yo Yamaguchi, Takuma Kawasaki, Akihiko Hara, Takashi Todo, Naoshi Hiramatsu
Aquaculture, 583, 30 Mar. 2024
Scientific journal

Variation in responses to photoperiods and temperatures in Japanese medaka from different latitudes.
Ai Shinomiya, Daisuke Adachi, Tsuyoshi Shimmura, Miki Tanikawa, Naoshi Hiramatsu, Shigeho Ijiri, Kiyoshi Naruse, Mitsuru Sakaizumi, Takashi Yoshimura
Zoological letters, 9, 1, 16, 16, 22 Jul. 2023, [International Magazine]
English, Scientific journal, Seasonal changes are more robust and dynamic at higher latitudes than at lower latitudes, and animals sense seasonal changes in the environment and alter their physiology and behavior to better adapt to harsh winter conditions. However, the genetic basis for sensing seasonal changes, including the photoperiod and temperature, remains unclear. Medaka (Oryzias latipes species complex), widely distributed from subtropical to cool-temperate regions throughout the Japanese archipelago, provides an excellent model to tackle this subject. In this study, we examined the critical photoperiods and critical temperatures required for seasonal gonadal development in female medaka from local populations at various latitudes. Intraspecific differences in critical photoperiods and temperatures were detected, demonstrating that these differences were genetically controlled. Most medaka populations could perceive the difference between photoperiods for at least 1 h. Populations in the Northern Japanese group required 14 h of light in a 24 h photoperiod to develop their ovaries, whereas ovaries from the Southern Japanese group developed under 13 h of light. Additionally, Miyazaki and Ginoza populations from lower latitudes were able to spawn under short-day conditions of 11 and 10 h of light, respectively. Investigation of the critical temperature demonstrated that the Higashidori population, the population from the northernmost region of medaka habitats, had a critical temperature of over 18 °C, which was the highest critical temperature among the populations examined. The Miyazaki and the Ginoza populations, in contrast, were found to have critical temperatures under 14 °C. When we conducted a transplant experiment in a high-latitudinal environment using medaka populations with different seasonal responses, the population from higher latitudes, which had a longer critical photoperiod and a higher critical temperature, showed a slower reproductive onset but quickly reached a peak of ovarian size. The current findings show that low latitudinal populations are less responsive to photoperiodic and temperature changes, implying that variations in this responsiveness can alter seasonal timing of reproduction and change fitness to natural environments with varying harshnesses of seasonal changes. Local medaka populations will contribute to elucidating the genetic basis of seasonal time perception and adaptation to environmental changes.

Identification and characterization of lipocalin-type prostaglandin D2 synthase homologs in the urine of male rockfish
Yo Yamaguchi, Jin Namgung, Jun Nagata, Takuma Kawasaki, Akihiko Hara, Takashi Todo, Naoshi Hiramatsu
Gene, 147093, 147093, Elsevier BV, Dec. 2022, [Peer-reviewed]
Scientific journal

Molecular characterization of fshb and lhb subunits and their expression profiles in captive white-edged rockfish, Sebastes taczanowskii.
Yo Yamaguchi, Jun Nagata, Osamu Nishimiya, Takuma Kawasaki, Naoshi Hiramatsu, Takashi Todo
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 261, 111055, 111055, Nov. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Fundamental knowledge on the regulation of reproduction by gonadotropins (Gths) is quite limited in viviparous fishes. In the present study, we performed molecular cloning and characterization of cDNAs encoding two Gth subunits (fshb and lhb) from the pituitaries of viviparous white-edged rockfish, Sebastes taczanowskii; expression profiles of both gene transcripts were elucidated in the pituitaries of reproductive males and females which were kept in a captive environment. The cloned fshb and lhb fragments exhibited high sequence identities with corresponding β-subunit sequences from black rockfish, S. schlegelii. Notably, the fshb of white-edged rockfish appeared to lack a putative N-glycosylation site, whereas lhb conserved it. Expression of fshb and lhb transcripts in the rockfish pituitaries largely changed in synchrony but for minor exceptions. In males, levels of both transcripts increased with progression of spermatogenesis, although the peak for fshb (October) appeared slightly earlier than that for lhb (November). In females, both gene transcripts exhibited synchronous bimodal changes. High expression of fshb and lhb transcripts in the female pituitary during the gestation period, followed by the drastic decrease at parturition, suggest their possible involvement in regulation of gestation of this species. The knowledge gained for Sebastes in this study superimposes fundamental information necessary for further physiological understanding of viviparity in teleost fish.

Hepatic estrogen-responsive genes relating to oogenesis in cutthroat trout (Oncorhynchus clarki): The transcriptional induction in primary cultured hepatocytes and the in vitro promoter transactivation in responses to estradiol-17β.
Jun Nagata, Yuji Mushirobira, Osamu Nishimiya, You Yamaguchi, Toshiaki Fujita, Naoshi Hiramatsu, Akihiko Hara, Takashi Todo
General and comparative endocrinology, 310, 113812, 113812, 01 Sep. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Estradiol-17β (E2) regulates transcription of estrogen-responsive genes via estrogen receptors (Esr). In many teleost species, choriogenin (chg), vitellogenin (vtg) and esr genes are transactivated by E2 in the liver. This study aimed i) to compare expression properties of all subtypes of these genes (chg: chgHα, chgHβ, chgL; vtg: vtgAs, vtgC; esr: esr1a, esr1b, esr2a, esr2b) in response to estrogen stimulation, and ii) to confirm how each of four Esr subtypes is involved in the transcriptional regulation of these estrogen-responsive genes in cutthroat trout hepatocytes. In hepatocytes in primary culture, all chg and vtg subtype mRNA levels, and those of esr1a, were increased by E2 treatment (10-6 M) at 24 and 72 h post initiation (hpi), but esr1b, esr2a and esr2b mRNA levels were not. Treatment of hepatocytes with various concentrations of E2 (10-11-10-6 M) induced dose-dependent increases in the levels of all chg and vtg subtype mRNAs at 24 and 72 hpi. At both time points, the lowest dose that induced a significant increase in the expression levels of mRNAs (LOEC) for E2 differed among the genes; LOECs were estimated as 10-11 M for chgHα at 24 hpi, as 10-9 M for vtgC at 72 hpi, and as 10-10 M for other mRNAs at both 24 and 72 hpi. Meanwhile, the levels of esr1a mRNA exhibited a dose-dependent increase at 24 and 72 hpi, but the LOEC shifted from 10-9 M at 24 hpi to 10-7 M at 72 hpi because of a decrease in mRNA levels at treatment groups exposed to high concentrations of E2. All Esr subtypes transactivated chg, vtg and esr1a promoters in the presence of E2 in vitro. The activation levels indicated that promoter activity of chgHα ≥ vtgAs > chgHβ > chgL ≥ vtgC ≥ esr1a when mediated by Esr1a, chgHβ > chgHα > chgHL > vtgAs ≥ vtgC ≥ esr1a by Esr1b, chgHβ ≥ chgL > chgHα ≥ vtgAs > vtgC > esr1a by Esr2a, and chgHβ ≥ chgHα ≥ vtgAs > chgL ≥ vtgC > esr1a by Esr2b. Collectively, different Esr subtypes were distinctly different in their ability to transactivate estrogen-responsive target genes, resulting in differential expression of chg, vtg and esr1a genes in the estrogen-exposed hepatocytes.

Changes in the Hepatic Expression of Three Vitellogenin Subtype Genes During Ovarian Development in Female White-Edged Rockfish (Sebastes taczanowskii)
Jun Nagata, Satoru Wada, Osamu Nishimiya, Meiqin Wu, Yuji Mushirobira, Yo Yamaguchi, Takanori Yokono, Takuma Kawasaki, Takahiro Matsubara, Takashi Todo, Akihiko Hara, Naoshi Hiramatsu
Zoological Science, 38, 5, Zoological Society of Japan, 18 Aug. 2021
Scientific journal

Knock out of a major vitellogenin receptor gene with eight ligand binding repeats in medaka (Oryzias latipes) using the CRISPR/Cas9 system.
Jin Namgung, Hiroko Mizuta, Yo Yamaguchi, Jun Nagata, Takashi Todo, Ozlem Yilmaz, Naoshi Hiramatsu
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 257, 110967, 110967, Jul. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Recent studies of vitellogenesis engendered a novel model of teleost yolk formation in which multiple yolk precursors, vitellogenins (Vtgs), and their receptors (Vtgrs) interact to ensure proper yolk composition for embryonic development and larval growth. As a step toward verification of this concept, we examined the role of one candidate Vtgr, termed low-density lipoprotein receptor relative with eight ligand-binding repeat (Lr8), in the medaka, a representative teleost and established laboratory model. A homozygous lr8 knock out (lr8-KO) medaka was produced to perform reverse-genetic functional analyses. In ovaries of wild type (WT) medaka, Western blotting detected a putative Lr8 protein band at ~130 kDa, while immunohistochemistry detected the putative Lr8 signal at the periphery of the oocyte underneath the zona radiata. These signals disappeared in ovaries of the lr8-KO group. Offspring of lr8-KO medaka exhibited decreased survival rate compared to WT fish, but KO of lr8 was not 100% lethal. There was no significant difference in total yolk protein content or size of eggs between WT and lr8-KO fish. However, LC-MS/MS analyses revealed a remarkable decrease in the relative abundance of yolk proteins derived from VtgAb in lr8-KO eggs, in conjunction with a compensatory increase in proteins derived from VtgAa1. These findings strongly support the conclusion that Lr8 is an important receptor for VtgAb in medaka. The disruption of proper yolk composition by lr8-KO is possibly one cause of the low offspring survival.

Development and characterization of polyclonal antibodies against subtype specific vitellogenin of the dojo loach, Misgurnus anguillicaudatus
Weilong Wang, Qingping Lian, Yuange Chen, Naoshi Hiramatsu, Meiqin Wu
AQUACULTURE, 532, Feb. 2021, [Peer-reviewed]
English, Scientific journal

Development of a species identification method for shishamo smelt larvae using whole-mount immunohistochemistry
莚平 裕次, 川崎 琢真, 中田 訓彰, 竹中 映美, 永田 淳, 石田 良太郎, 山口 浩志, 佐藤 充, 東藤 孝, 平松 尚志
水産増殖 = Aquaculture science, 68, 1, 1, 8, 日本水産増殖学会, Apr. 2020, [Peer-reviewed]
Japanese

Development of specific enzyme-linked immunosorbent assays for multiple vitellogenins in marbled sole, Pleuronectes yokohamae
Haruna Amano, Seiichi Uno, Jiro Koyama, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 281, 67, 72, Sep. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Synergistic effects of estradiol and 11-ketotestosterone on vitellogenin physiology in the shortfinned eel (Anguilla australis)
Georgia Thomson-Laing, Erin L. Damsteegt, Jun Nagata, Shigeho Ijiri, Shinji Adachi, Takashi Todo, Naoshi Hiramatsu, P. Mark Lokman
BIOLOGY OF REPRODUCTION, 100, 5, 1319, 1332, May 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Development of specific chemiluminescent immunoassays for three subtypes of vitellogenin in grey mullet (Mugil cephalus)
Haruna Amano, Akihiro Kotake, Naoshi Hiramatsu, Toshiaki Fujita, Takashi Todo, Jun-ya Aoki, Kiyoshi Soyano, Hirohiko Kagawa, Akihiko Hara
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 271, 30, 38, Jan. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Oogenesis and Egg Quality in Finfish: Yolk Formation and Other Factors Influencing Female Fertility
Benjamin Reading, Linnea Andersen, Yong-Woon Ryu, Yuji Mushirobira, Takashi Todo, Naoshi Hiramatsu
Fishes, 3, 4, 45, 45, MDPI AG, 21 Nov. 2018, [Peer-reviewed]
English, Scientific journal, Egg quality in fishes has been a topic of research in aquaculture and fisheries for decades as it represents an important life history trait and is critical for captive propagation and successful recruitment. A major factor influencing egg quality is proper yolk formation, as most fishes are oviparous and the developing offspring are entirely dependent on stored egg yolk for nutritional sustenance. These maternally derived nutrients consist of proteins, carbohydrates, lipids, vitamins, minerals, and ions that are transported from the liver to the ovary by lipoprotein particles including vitellogenins. The yolk composition may be influenced by broodstock diet, husbandry, and other intrinsic and extrinsic conditions. In addition, a number of other maternal factors that may influence egg quality also are stored in eggs, such as gene transcripts, that direct early embryonic development. Dysfunctional regulation of gene or protein expression may lead to poor quality eggs and failure to thrive within hours of fertilization. These gene transcripts may provide important markers as their expression levels may be used to screen broodstock for potential spawning success. In addition to such intrinsic factors, stress may lead to ovarian atresia or reproductive failure and can impact fish behavior, fecundity, and ovulation rate. Finally, postovulatory aging may occur when eggs become overripe and the fish fails to spawn in a timely fashion, leading to low fertility, often encountered during manual strip spawning of fish.

Molecular cloning of vitellogenin gene promoters and in vitro and in vivo transcription profiles following estradiol-17 beta administration in the cutthroat trout
Yuji Mushirobira, Osamu Nishimiya, Jun Nagata, Takashi Todo, Akihiko Hara, Benjamin J. Reading, Naoshi Hiramatsu
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 267, 157, 166, Oct. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Production of recombinant salmon insulin-like growth factor binding protein-1 subtypes
Hanae Tanaka, Gakuto Oishi, Yusuke Nakano, Hiroko Mizuta, Yuta Nagano, Naoshi Hiramatsu, Hironori Ando, Munetaka Shimizu
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 257, 184, 191, Feb. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

Species-identification of eggs of three flounders by whole-mount immunostaining
Nagata Jun, Todo Takashi, Hara Akihiko, Hiramatsu Naoshi, Kasai Kei, Mineno Kazuhiro, Fujisaki Yudai, Mushirobira Yuji, Namgung Jin, Takeda Yasutaka, Fujita Toshiaki, Kawasaki Takuma
Aquaculture Science, 66, 4, 257, 266, Japanese Society for Aquaculture Science, 2018, [Peer-reviewed]
Japanese, Whole-mount immunostaining (WI) was used for identification of eggs of brown sole (Pseudopleuronectes herzensteini), sand flounder (Limanda punctatissima) and pointhead flounder (Cleisthenes pinetorum). Polyclonal antibodies were raised in rabbit against vitelline envelope (VE) of ovulated eggs of each flounder (a-brown sole VE, a-sand flounder VE and a-pointhead flounder VE). A WI method was first developed using the non-labeled primary a-VE antibodies in conjunction with a labeled-secondary antibody (2-step method). Another WI method using the labeled-primary a-VE anitbodies alone was developed (1-step method) in order to omit the use of the secondary antibody. When fertilized eggs of each species (2-24 hrs post-fertilization) were examined, both WI methods effectively stained the eggs in a species-specific manner. Immunological tools developed for identification of flounder eggs in this study will contribute to simplify fishery surveys of flounder species during their early life stages.

Expression analysis of estradiol-17β responsive genes in the liver of female cutthroat trout (Oncorhynchus clarki) during a reproductive cycle
Nagata Jun, Mushirobira Yuji, Nishimiya Osamu, Fujita Toshiaki, Hiramatsu Naoshi, Hara Akihiko, Todo Takashi
Aquaculture Science, 66, 2, 91, 101, Japanese Society for Aquaculture Science, 2018, [Peer-reviewed]
Japanese, Choriogenin (Chg) and vitellogenin (Vtg) subtypes and estrogen receptor α1 (Erα1) are upregulated by estradiol-17β (E2) in the liver of female salmonids. The aim of this study was to examine the relationships among hepatic mRNA levels for chgs (chgHα; chgHβ; chgL), vtgs (vtgAs; vtgC), erα1 and serum E2 levels in female cutthroat trout (Oncorhynchus clarki) during a reproductive cycle. Levels of serum E2 and hepatic chg mRNAs, as well as hepatic vtg mRNAs, increased in correlation with the progress of vitellogenesis. In the ovulated fish, chg mRNA remained at high levels while serum E2 and vtg mRNA levels decreased. Hepatic erα1 mRNA levels exhibited a peak in August (at the beginning of vitellogenesis) before levels of E2, chg and vtg mRNAs start to increase. These results suggest that expression levels of chg, vtg and erα1 genes are potentially regulated through E2 stimulation by different mechanisms.

Ovarian expression and localization of clathrin (Cltc) components in cutthroat trout, Oncorhynchus clarki: Evidence for Cltc involvement in endocytosis of vitellogenin during oocyte growth
Hiroko Mizuta, Yuji Mushirobira, Jun Nagata, Takashi Todo, Akihiko Hara, Benjamin J. Reading, Craig V. Sullivan, Naoshi Hiramatsu
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 212, 24, 34, Oct. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

ヌタウナギ2種エストロジェン受容体（ER）のcDNAクローニングと機能解析
西宮 攻, 勝 義直, 平松 尚志, 東藤 孝
比較内分泌学, 43, 160, 21, 23, 日本比較内分泌学会, 2017
Japanese

Development of artificial insemination techniques for viviparous black rockfish (Sebastes schlegelii)
Kawasaki Takuma, Shimizu Yohei, Mori Tatsunari, Hiramatsu Naoshi, Todo Takashi
Aquaculture Science, 65, 1, 73, 82, Japanese Society for Aquaculture Science, 2017, [Peer-reviewed]
English, Black rockfish (Sebastes schlegelii) is an economically important aquaculture species in Japan. Because it is a viviparous species, seedling production is typically performed using natural mating and the numbers of larva that are produced tend to be unstable. In the present study, we aimed to develop artificial insemination techniques for black rockfish to ensure stable seedling production. We used 20 female and 15 male mature individuals and examined when artificial insemination is best performed, in which diluent the sperm is best diluted and whether sperm collected from live or dead males both have fertilizing capability. The number of larvae produced by each pregnant female was counted. In addition, microsatellite markers were used to determine the relationship between larvae and parents. Following artificial insemination, 12 of 20 females became pregnant. The average litter size per pregnant female was about 130,000 fry. Pregnant females were obtained under all experimental conditions, regardless of season, sperm diluent and whether the male was live or dead. Paternity testing revealed that all larvae were produced by artificial insemination. Furthermore, females artificially inseminated by several males appeared to produce offspring derived from all males. The present study demonstrates that artificial insemination is possible in marine viviparous fish.

Molecular cloning and characterization of hagfish estrogen receptors
Osamu Nishimiya, Yoshinao Katsu, Hiroyuki Inagawa, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 165, Pt B, 190, 201, Jan. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Vitellogenesis and choriogenesis in fishes
Akihiko Hara, Naoshi Hiramatsu, Toshiaki Fujita
FISHERIES SCIENCE, 82, 2, 187, 202, Mar. 2016, [Peer-reviewed]
English

Molecular cloning of cDNAs encoding multiple vitellogenins in Amur sturgeon Acipenser schrenckii
Maebayashi Mamoru, Hiramatsu Naoshi, Inaoka Yuhei, Yoshida Tatsuya, Hagihara Seishi, Nishimiya Osamu, Mushirobira Yuji, Adachi Shinji, Hara Akihiko, Todo Takashi
Aquaculture Science, 64, 1, 63, 76, Japanese Society for Aquaculture Science, 2016, [Peer-reviewed]
English, Molecular cloning of cDNAs encoding an egg yolk precursor, vitellogenin (Vtg), was performed using the liver of estrogen-treated Amur sturgeon. Three kinds of vtg cDNA were cloned and temporarily named as vtg1, vtg2 and vtg3. These vtg cDNAs were obtained as contiguous sequences; each of vtg1, vtg2 and vtg3 sequences contained a complete open reading frame (5,307, 5,247 and 5,319 bp, respectively), each encoding 1,769, 1,749 and 1,773 aa residues, respectively. Similarity among amino acid sequences deduced from the three vtg cDNAs were relatively low, ranging from 64.3% to 47.9%. Three Vtgs appeared to be a complete-type, consisting of all representative yolk protein domains. Phylogenetic analysis revealed that Amur sturgeon Vtg1 formed a clade together with a published white sturgeon Vtg, while Amur sturgeon Vtg2 and Vtg3 formed another distinct clade. The results of phylogenetic analysis confirmed all three Vtgs belong to VtgAB type; vtg1/Vtg1, vtg2/Vtg2 and vtg3/Vtg3 were hereby designated as vtgAB1/VtgAB1, vtgAB2a/VtgAB2a and vtgAB2b/VtgAB2b, respectively. This study provided a basis to understand multiplicity in sturgeon vtg/Vtg and set a stage for their future application as reproductive biomarkers in sturgeon aquaculture.

Molecular Cloning and Partial Characterization of a Low-Density Lipoprotein Receptor-Related Protein 13 (Lrp13) Involved in Vitellogenin Uptake in the Cutthroat Trout (Oncorhynchus clarki)
Yuji Mushirobira, Hiroko Mizuta, Wenshu Luo, Takashi Todo, Akihiko Hara, Benjamin J. Reading, Craig V. Sullivan, Naoshi Hiramatsu
MOLECULAR REPRODUCTION AND DEVELOPMENT, 82, 12, 986, 1000, Dec. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins
Naoshi Hiramatsu, Takashi Todo, Craig V. Sullivan, Justin Schilling, Benjamin J. Reading, Takahiro Matsubara, Yong-Woon Ryu, Hiroko Mizuta, Wenshu Luo, Osamu Nishimiya, Meiqin Wu, Yuji Mushirobira, Ozlem Yilmaz, Akihiko Hara
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 221, 9, 15, Sep. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

How do eggs get fat? Insights into ovarian fatty acid accumulation in the shortfinned eel, Anguilla australis
Erin L. Damsteegt, Hiroko Mizuta, Naoshi Hiramatsu, P. Mark Lokman
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 221, 94, 100, Sep. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Machine learning reveals sex-specific 17-estradiol-responsive expression patterns in white perch (Morone americana) plasma proteins
Justin Schilling, Angelito I. Nepomuceno, Antonio Planchart, Jeffrey A. Yoder, Robert M. Kelly, David C. Muddiman, Harry V. Daniels, Naoshi Hiramatsu, Benjamin J. Reading
PROTEOMICS, 15, 15, 2678, 2690, Aug. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes
Benjamin J. Reading, Naoshi Hiramatsu, Justin Schilling, Katelyn T. Molloy, Norm Glassbrook, Hiroko Mizuta, Wenshu Luo, David A. Baltzegar, Valerie N. Williams, Takashi Todo, Akihiko Hara, Craig V. Sullivan
JOURNAL OF LIPID RESEARCH, 55, 11, 2287, 2295, Nov. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Proportional Accumulation of Yolk Proteins Derived From Multiple Vitellogenins is Precisely Regulated During Vitellogenesis in Striped Bass (Morone saxatilis)
Valerie N. Williams, Benjamin J. Reading, Haruna Amano, Naoshi Hiramatsu, Justin Schilling, Scott A. Salger, Taufika Islam Williams, Kevin Gross, Craig V. Sullivan
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY, 321, 6, 301, 315, Jul. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Development and partial characterisation of an antiserum against apolipoprotein B of the short-finned eel, Anguilla australis
Erin L. Damsteegt, Hiroko Mizuta, Yuichi Ozaki, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara, Shigeho Ijiri, Shinji Adachi, P. Mark Lokman
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 184, 5, 589, 599, Jul. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal

Multiple vitellogenins and product yolk proteins in striped bass, Morone saxatilis: molecular characterization and processing during oocyte growth and maturation
V. N. Williams, B. J. Reading, N. Hiramatsu, H. Amano, N. Glassbrook, A. Hara, C. V. Sullivan
FISH PHYSIOLOGY AND BIOCHEMISTRY, 40, 2, 395, 415, Apr. 2014, [Peer-reviewed]
English, Scientific journal

Molecular Cloning and Characterization of the Expression Profiles of Vitellogenin Transcripts in the Dojo Loach (Misgurnus anguillicaudatus) in Response to 17 alpha-ethinylestradioland 17 beta-estradiol Administration
Meiqin Wu, Osamu Nishimiya, Misato Nakamori, Kiyoshi Soyano, Takashi Todo, Akihiko Hara, Naoshi Hiramatsu
ZOOLOGICAL SCIENCE, 31, 4, 202, 212, Apr. 2014, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Biochemical and Immunochemical Characterization of Two Discrete Vitellogenin Proteins and Their Derived Lipovitellins in the Inshore Hagfish (Eptatretus burgeri)
Osamu Nishimiya, Yasuyuki Kunihiro, Naoshi Hiramatsu, Hiroyuki Inagawa, Takashi Todo, Akihiko Hara
ZOOLOGICAL SCIENCE, 31, 4, 251, 257, Apr. 2014, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Molecular cloning and partial characterization of an ovarian receptor with seven ligand binding repeats, an orthologue of low-density lipoprotein receptor, in the cutthroat trout (Oncorhynchus clarki)
Wenshu Luo, Yuta Ito, Hiroko Mizuta, Kiyohiro Massaki, Naoshi Hiramatsu, Takashi Todo, Benjamin J. Reading, Craig V. Sullivan, Akihiko Hara
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 166, 2, 263, 271, Oct. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Ovarian expression and localization of a vitellogenin receptor with eight ligand binding repeats in the cutthroat trout (Oncorhynchus clarki)
Hiroko Mizuta, Wenshu Luo, Yuta Ito, Yuji Mushirobira, Takashi Todo, Akihiko Hara, Benjamin J. Reading, Craig V. Sullivan, Naoshi Hiramatsu
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 166, 1, 81, 90, Sep. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Characterization of Alpha-fetoprotein Levels in Three Dolphin Species: Development of Sensitive Immunoassays for Analysis of the Pregnancy-associated Variations
Yuka Morita, Naoshi Hiramatsu, Toshiaki Fujita, Haruna Amano, Etsuko Katsumata, Kazutoshi Arai, Toshihide Iwasaki, Takashi Todo, Akihiko Hara
JOURNAL OF REPRODUCTION AND DEVELOPMENT, 59, 4, 368, 377, Aug. 2013, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Characterization of vitellogenin and its derived yolk proteins in cloudy catshark (Scyliorhinus torazame)
Kodai Yamane, Tomoki Yagai, Osamu Nishimiya, Rieko Sugawara, Haruna Amano, Toshiaki Fujita, Naoshi Hiramatsu, Takashi Todo, Takahiro Matsubara, Akihiko Hara
FISH PHYSIOLOGY AND BIOCHEMISTRY, 39, 2, 373, 390, Apr. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Molecular Cloning and Transcript Expression of Genes Encoding Two Types of Lipoprotein Lipase in the Ovary of Cutthroat Trout, Oncorhynchus clarki
Yong-Woon Ryu, Ricako Tanaka, Ayumi Kasahara, Yuta Ito, Naoshi Hiramatsu, Takashi Todo, Craig V. Sullivan, Akihiko Hara
ZOOLOGICAL SCIENCE, 30, 3, 224, 237, Mar. 2013, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Changes in levels of dual vitellogenin transcripts and proteins in cutthroat trout Oncorhynchus clarki during ovarian development
Yuji Mushirobira, Hiroko Mizuta, Wenshu Luo, Yuka Morita, Sayumi Sawaguchi, Takahiro Matsubara, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara
NIPPON SUISAN GAKKAISHI, 79, 2, 175, 189, Mar. 2013, [Peer-reviewed]
Japanese, Scientific journal

Multiple ovarian lipoprotein receptors in teleosts
N. Hiramatsu, W. Luo, B. J. Reading, C. V. Sullivan, H. Mizuta, Y. -W. Ryu, O. Nishimiya, T. Todo, A. Hara
FISH PHYSIOLOGY AND BIOCHEMISTRY, 39, 1, 29, 32, Feb. 2013, [Peer-reviewed]
English, Scientific journal

Relationships between gill Na+,K+-ATPase activity and endocrine and local insulin-like growth factor-I levels during smoltification of masu salmon (Oncorhynchus masou)
Takahiro Shimomura, Takuro Nakajima, Moeri Horikoshi, Anai Iijima, Hirokazu Urabe, Shinya Mizuno, Naoshi Hiramatsu, Akihiko Hara, Munetaka Shimizu
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 178, 2, 427, 435, Sep. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effects of ethynylestradiol on vitellogenin synthesis and sex differentiation in juvenile grey mullet (Mugil cephalus) persist after long-term exposure to a clean environment
Jun-ya Aoki, Ayaka Hatsuyama, Naoshi Hiramatsu, Kiyoshi Soyano
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 154, 4, 346, 352, Nov. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

スジイルカにおけるpregnancy‐associated glycoprotein cDNAのクローニング
盛田祐加, 平松尚志, 岩崎俊秀, 東藤孝, 原彰彦
J Reprod Dev, 57, Suppl Japanese Issue, J174, 20 Aug. 2011, [Peer-reviewed]
Japanese

Characterization of Alpha-fetoprotein in Fetal Striped Dolphin (Stenella coeruleoalba): Purification of Protein Product and Molecular Cloning of the Corresponding Transcript
Yuka Morita, Naoshi Hiramatsu, Toshiaki Fujita, Haruna Amano, Takashi Todo, Akihiko Hara
ZOOLOGICAL SCIENCE, 28, 3, 215, 224, Mar. 2011, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Disparate Binding of Three Types of Vitellogenin to Multiple Forms of Vitellogenin Receptor in White Perch
Benjamin J. Reading, Naoshi Hiramatsu, Craig V. Sullivan
BIOLOGY OF REPRODUCTION, 84, 2, 392, 399, Feb. 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

Evaluation of environmental estrogenic activities in the Yangtze River estuary: utilization of vitellogenin production in red lip mullet.
Mei-qin WU, Lei HONG, Wenshu LUO, Naoshi HIRAMATSU, Junya AOKI, Kiyoshi SOYANO, Jun-sheng ZHONG, Akihiko HARA
Acuaculture Sci., 59, 1, 65, 73, 水産増殖談話会, 2011, [Peer-reviewed]
English, 本研究では、中国揚子江河口における3つの汽水域について、エストロジェン活性の有無を調査した。調査はメナダ（Chelon haematocheilus）を用い、本種のエストロジェン感受性蛋白であるビテロジェニンBサブタイプ（VgB）の血中レベルと肝臓でのmRNA発現をマーカーとし、化学発光免疫測定法（CLIA）とリアルタイムPCR測定系によりこれらを測定した。同調査域から捕獲されたメナダにおいて、VgB蛋白およびmRNAの発現は、測定系の検出限界以下もしくは極めて低く、人工海水環境で順致された個体から得たコントロール値とほぼ同一であった。また、同捕獲個体の生殖腺は、40個体中1個体に精巣卵が観察された以外、異常な形態を示すものは全く無かった。このことより調査域はエストロジェン活性に関しては殆ど危険性の無い状態にあると判断された。

Purification and characterization of a novel incomplete-type vitellogenin protein (VgC) in Sakhalin taimen (Hucho perryi)
Haruna Amano, Machiko Mochizuki, Toshiaki Fujita, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 157, 1, 41, 48, Sep. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Studies on yolk precursors and their receptors involved in fish oogenesis
Naoshi Hiramatsu
NIPPON SUISAN GAKKAISHI, 76, 4, 613, 616, Jul. 2010
Japanese, Scientific journal

Development of chemiluminescent immunoassay for vitellogenin in red lip mullet, Liza haematocheila, Journal of fisheries of China
Wu M, Wada T, Luo W, Morita Y, Hiramatsu N, Hara A, Zhong J
Journal of fisheries of China, 34, 4, 581, 589, 2010, [Peer-reviewed]
Chinese

Purification of multiple precursors for egg chorion proteins in Atlantic cod (Gadus morhua).
Toshiaki Fujita, Alexander P Scott, Loanna Katsiadaki, Haruna Amano, Lei Hong, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara
Zoological science, 26, 12, 870, 7, Dec. 2009, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Induction of vitellogenin production in male tilapia (Oreochromis mossambicus) by commercial fish diets.
Lori K Davis, Bradley K Fox, Chhorn Lim, Naoshi Hiramatsu, Craig V Sullivan, Tetsuya Hirano, E Gordon Grau
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 154, 2, 249, 54, Oct. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Purification and Classification of Three Lipovitellin Subtypes in the Marbled Sole (Pleuronectes yokohamae)
Haruna Amano, Toshiaki Fujita, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara
ZOOLOGICAL SCIENCE, 26, 7, 510, 516, Jul. 2009, [Peer-reviewed]
English, Scientific journal

Effects of o,p'-DDE, heptachlor, and 17beta-estradiol on vitellogenin gene expression and the growth hormone/insulin-like growth factor-I axis in the tilapia, Oreochromis mossambicus.
Lori K Davis, Nancy Visitacion, Larry G Riley, Naoshi Hiramatsu, Craig V Sullivan, Tetsuya Hirano, E Gordon Grau
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 149, 4, 507, 14, May 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Conserved and Variant Molecular and Functional Features of Multiple Egg Yolk Precursor Proteins (Vitellogenins) in White Perch (Morone americana) and other Teleosts
Benjamin J. Reading, Naoshi Hiramatsu, Sayumi Sawaguchi, Takahiro Matsubara, Akihiko Hara, Mark O. Lively, Craig V. Sullivan
MARINE BIOTECHNOLOGY, 11, 2, 169, 187, Apr. 2009, [Peer-reviewed]
English

Choriogenin and vitellogenin in red lip mullet (Chelon haematocheilus): purification, characterization, and evaluation as potential biomarkers for detecting estrogenic activity.
Lei Hong, Toshiaki Fujita, Tatsunori Wada, Haruna Amano, Naoshi Hiramatsu, Xiumei Zhang, Takashi Todo, Akihiko Hara
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 149, 1, 9, 17, Jan. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Multiple vitellogenin-derived yolk proteins in gray mullet (Mugil cephalus): disparate proteolytic patterns associated with ovarian follicle maturation.
Haruna Amano, Toshiaki Fujita, Naoshi Hiramatsu, Hirohiko Kagawa, Takahiro Matsubara, Craig V Sullivan, Akihiko Hara
Molecular reproduction and development, 75, 8, 1307, 17, Aug. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Molecular cloning and characterization of three distinct choriogenins in masu salmon, Oncorhynchus masou.
Toshiaki Fujita, Haruhisa Fukada, Munetaka Shimizu, Naoshi Hiramatsu, Akihiko Hara
Molecular reproduction and development, 75, 7, 1217, 28, Jul. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Molecular alteration of three forms of vitellogenins and their product yolk proteins during oocyte growth and maturation in grey mullet (Mugil cephalus)
Haruna Amano, Toshiaki Fujita, Naoshi Hiramatsu, Hirohiko Kagawa, Sayumi Sawaguchi, Takahiro Matsubara, Craig V. Sullivan, Akihiko Hara
CYBIUM, 32, 2, 156, 158, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Deduced primary structures of three vitellogenins and specific binding to putative multiple ovarian receptors in white perch (Morone americana)
Benjamin J. Reading, Naoshi Hiramatsu, Takahiro Matsubara, Akihiko Hara, Craig V. Sullivan
CYBIUM, 32, 2, 159, 161, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Estrogen regulation of multiple vitellogenin and estrogen receptor genes and of the growth hormone-insulin like growth factor axis in tilapia
Lori Davis, Naoshi Hiramatsu, Craig Sullivan, Tetsuya Hirano, E. Gordon Grau
CYBIUM, 32, 2, 242, 243, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Differential production and uptake of dual vitellogenins in Japanese medaka (Oryzias latipes)
Naoshi Hiramatsu, Mihoka Inoue, Hiroki Ideuchi, Toshiaki Fujita, Haruna Amano, Takahiro Matsubara, Craig V. Sullivan, Akihiko Hara
CYBIUM, 32, 2, 260, 260, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Controlled accumulation of multiple vitellogenins into oocytes during vitellogenesis in the barfin flounder, Verasper moseri
Sayumi Sawaguchi, Nobuyuki Ohkuio, Haruna Amano, Naoshi Hiramatsu, Akihiko Hara, Craig V. Sullivan, Takahiro Matsubara
CYBIUM, 32, 2, 262, 262, Jul. 2008, [Peer-reviewed]
English, Scientific journal

Gender-specific expression of multiple estrogen receptors, growth hormone receptors, insulin-like growth factors and vitellogenins, and effects of 17 beta-estradiol in the male tilapia (Oreochromis mossambicus).
Lori K Davis, Andrew L Pierce, Naoshi Hiramatsu, Craig V Sullivan, Tetsuya Hirano, E Gordon Grau
General and comparative endocrinology, 156, 3, 544, 51, 01 May 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

Purification of multiple vitellogenins in grey mullet (Mugil cephalus)
Haruna Amano, Toshiaki Fujita, Naoshi Hiramatsu, Sayumi Sawaguchi, Takahiro Matsubara, Craig V. Sullivan, Akihiko Hara
MARINE BIOLOGY, 152, 6, 1215, 1225, Nov. 2007, [Peer-reviewed]
English, Scientific journal

Induction of three vitellogenins by 17beta-estradiol with concurrent inhibition of the growth hormone-insulin-like growth factor 1 axis in a euryhaline teleost, the tilapia (Oreochromis mossambicus).
Lori K Davis, Naoshi Hiramatsu, Kaori Hiramatsu, Benjamin J Reading, Takahiro Matsubara, Akihiko Hara, Craig V Sullivan, Andrew L Pierce, Tetsuya Hirano, E Gordon Grau
Biology of reproduction, 77, 4, 614, 25, Oct. 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Egg yolk proteins in gray mullet (Mugil cephalus): Purification and classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and molecular cloning of the parent vitellogenin genes
Haruna Amano, Toshiaki Fujita, Naoshi Hiramatsu, Munetaka Shimizu, Sayumi Sawaguch, Takahiro Iatsubara, Hirohiko Kagawa, Masaki Nagae, Craig V. Sullivan, Akihiko Hara
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY, 307A, 6, 324, 341, Jun. 2007, [Peer-reviewed]
English, Scientific journal

Molecular characterization of three forms of vitellogenin and their yolk protein products during oocyte growth and maturation in red seabream (Pagrus major), a marine teleost spawning pelagic eggs
S Sawaguchi, H Kagawa, N Ohkubo, N Hiramatsu, CV Sullivan, T Matsubara
MOLECULAR REPRODUCTION AND DEVELOPMENT, 73, 6, 719, 736, Jun. 2006, [Peer-reviewed]
English, Scientific journal

Multiple piscine vitellogenins: biomarkers of fish exposure to estrogenic endocrine disruptors in aquatic environments
N Hiramatsu, T Matsubara, T Fujita, CV Sullivan, A Hara
MARINE BIOLOGY, 149, 1, 35, 47, Apr. 2006, [Peer-reviewed]
English, Scientific journal

Annual changes in serum levels of two choriogenins and vitellogenin in masu salmon, Oncorhynchus masou.
Toshiaki Fujita, Haruhisa Fukada, Munetaka Shimizu, Naoshi Hiramatsu, Akihiko Hara
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 141, 2, 211, 7, Jun. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Multiple vitellogenins (Vgs) in mosquitofish (Gambusia affinis): identification and characterization of three functional Vg genes and their circulating and yolk protein products.
Sayumi Sawaguchi, Yasunori Koya, Norio Yoshizaki, Nobuyuki Ohkubo, Tadashi Andoh, Naoshi Hiramatsu, Craig V Sullivan, Akihiko Hara, Takahiro Matsubara
Biology of reproduction, 72, 4, 1045, 60, Apr. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Chapter 16 Vitellogenesis and endocrine disruption
Naoshi Hiramatsu, Ann O. Cheek, Craig V. Sullivan, Takahiro Matsubara, Akihiko Hara
Biochemistry and Molecular Biology of Fishes, 6, C, 431, 471, 2005, [Peer-reviewed]
English, Scientific journal

Identification, characterization and cDNA cloning of three forms of vitellogenin, two general forms and a phosvitinless form, in a viviparous mosquitofish, Gambusia affinis.
Sawaguchi S, Koya Y, Yoshizaki N, Ohkubo N, Andoh T, Sullivan C.V, Hiramatsu N, Hara A, Matsubara T
Biology of Reproduction, 72, 1045, 1060, 2005, [Peer-reviewed]

Molecular characterization and expression of vitellogenin receptor from white perch (Morone americana)
N Hiramatsu, RW Chapman, JK Lindzey, MR Haynes, CV Sullivan
BIOLOGY OF REPRODUCTION, 70, 6, 1720, 1730, Jun. 2004, [Peer-reviewed]
English, Scientific journal

Quantification of serum levels of precursors to vitelline envelope proteins (choriogenins) and vitellogenin in estrogen treated masu salmon, Oncorhynchus masou
T Fujita, H Fukada, M Shimizu, N Hiramatsu, A Hara
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 136, 1, 49, 57, Mar. 2004, [Peer-reviewed]
English, Scientific journal

Carp (Cyprinus carpio) vitellogenin: purification and development of a simultaneous chemiluminescent immunoassay
H Fukada, Y Fujiwara, T Takahashi, N Hiramatsu, CV Sullivan, A Hara
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 134, 3, 615, 623, Mar. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Atresia in temperate basses: cloning of hatching enzyme (choriolysin) homologues from atretic ovaries
DM Donato, N Hiramatsu, KM Arey, K Hiramatsu, AM Kennedy, CL Morton, A Hara, CV Sullivan
FISH PHYSIOLOGY AND BIOCHEMISTRY, 28, 1-4, 329, 330, 2003, [Peer-reviewed]
English, Scientific journal

Multiple vitellogenins in white perch (Morone americana)
K. Hiramatsu, N. Hiramatsu, A. Hara, T. Matsubara, C. V. Sullivan
Fish Physiology and Biochemistry, 28, 1-4, 347, 348, 2003, [Peer-reviewed]
English, Scientific journal

Induced maturation and spawning: opportunities and applications for research on oogenesis
CV Sullivan, N Hiramatsu, AM Kennedy, RW Clark, GM Weber, T Matsubara, A Hara
FISH PHYSIOLOGY AND BIOCHEMISTRY, 28, 1-4, 481, 486, 2003, [Peer-reviewed]
English, Scientific journal

Oocyte growth in temperate basses: multiple forms of vitellogenin and their receptor
N Hiramatsu, A Hara, T Matsubara, K Hiramatsu, CV Sullivan
FISH PHYSIOLOGY AND BIOCHEMISTRY, 28, 1-4, 301, 303, 2003, [Peer-reviewed]
English, Scientific journal

Multiple vitellogenins and their unique roles in marine teleosts
T Matsubara, M Nagae, N Ohkubo, T Andoh, S Sawaguchi, N Hiramatsu, CV Sullivan, A Hara
FISH PHYSIOLOGY AND BIOCHEMISTRY, 28, 1-4, 295, 299, 2003, [Peer-reviewed]
English, Scientific journal

Vitellogenin-derived yolk proteins of white perch, Morone americana: Purification, characterization, and vitellogenin-receptor binding
N Hiramatsu, A Hara, K Hiramatsu, H Fukada, GM Weber, ND Denslow, CV Sullivan
BIOLOGY OF REPRODUCTION, 67, 2, 655, 667, Aug. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

Purification of serum precursor proteins to vitelline envelope (choriogenins) in masu salmon, Oncorhynchus masou.
Toshiaki Fujita, Munetaka Shimizu, Naoshi Hiramatsu, Haruhisa Fukada, Akihiko Hara
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 132, 3, 599, 610, Jul. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

Vitellogenin-derived yolk proteins in a hybrid sturgeon, bester (Huso huso x Acipencer ruthenus): identification, characterization and course of proteolysis during embryogenesis.
Naoshi Hiramatsu, Kaori Hiramatsu, Kaori Hirano, Akihiko Hara
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 131, 2, 429, 41, Feb. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

Identification and characterization of proteases involved in specific proteolysis of vitellogenin and yolk proteins in salmonids
N Hiramatsu, N Ichikawa, H Fukada, T Fujita, GV Sullivan, A Hara
JOURNAL OF EXPERIMENTAL ZOOLOGY, 292, 1, 11, 25, Jan. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

Identification, purification and classification of multiple forms of vitellogenin from white perch (Morone americana)
N Hiramatsu, T Matsubara, A Hara, DM Donato, K Hiramatsu, ND Denslow, CV Sullivan
FISH PHYSIOLOGY AND BIOCHEMISTRY, 26, 4, 355, 370, 2002, [Peer-reviewed]
English, Scientific journal

Cloning of cDNA for choriogenin H and choriogenin L of masu salmon
Toshiaki Fujita, Haruhisa Fukada, Munetaka Shimizu, Naoshi Hiramatsu, Akihiko Hara
Fisheries Science, 68, 1273, 1274, 2002, [Peer-reviewed]
English, Scientific journal

Developmental changes in egg yolk proteins of walleye pollock, Theragra chalcogramma, and a comparative study of immunoreactivity of other north pacific teleosts and invertebrate eggs.
Bailey K. M, Merati N, Helser M, Hiramatsu N, Hara A
Bull. Fac. Fish. Sci. Hokkaido Univ, 53, 3, 95, 105, Hokkaido University, 2002, [Peer-reviewed]
English

Vitellogenesis in Aquatic Animals
Naoshi Hiramatsu, Takahiro Matsubara, Gregory M. Weber, Craig V. Sullivan, Akihiko Hara
FISHERIES SCIENCE, 68, 694, 699, 2002, [Peer-reviewed]
English, Scientific journal

Serum Immunoglobulin M (IgM) in Sakhalin Taimen (Hucho perryi): Purification, Caracterization, Circulating Levels, and Specific IgM Production by the Parasitic Salmincola stellatus
HIRAMATSU Naoshi, FUKADA Haruhisa, KITAMURA Makiko, SHIMIZU Munetaka, FUDA Hirotoshi, KOBAYASHI Kunihiko, HARA Akihiko
水産増殖, 49, 3, 347, 355, 日本水産増殖学会, 20 Sep. 2001, [Peer-reviewed]
English, Immunoglobulin M (IgM) was purified from the serum of Sakhalin taimen (Hucho perryi) by salting-out, ion-exchange chromatography on DEAF-cellulose, and gel filtration on Sepharose 6B. The intact, tetrameric taimen IgM has a mass of 750 kDa with molecular architecture typical of IgM from other salmonids. The molecular weights of heavy (μ) chain and light chains (L) of the IgM monomer were estimated to be 68 kDa and 23 kDa, respectively. Purified taimen IgM was used to raise a specific rabbit antiserum and to develop a single radial immunodiffusion assay for measuring circulating IgM levels. The serum IgM levels in captive, immature or maturing female taimen varied between 1 and 5 mg/ml, showing seasonal changes regardless of fish age, with relatively low levels in spring and conversely high levels in autumn. Production of specific serum IgM to a parasite, Salmincola stellatus, was assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The parasitized taimen serum could react with protein components of aqueous extracts from the parasite that were blotted on a nitrocellulose membrane after SDS-PAGE, but normal taimen serum did not, indicating that the parasitized fish produced the specific IgM to S. stellatus in the serum.

Development and validation of chemiluminescent immunoassay for vitellogenin in five salmonid species
H Fukada, A Haga, T Fujita, N Hiramatsu, CV Sullivan, A Hara
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY, 130, 1, 163, 170, Aug. 2001, [Peer-reviewed]
English, Scientific journal

Estimation of baseline vitellogenin level in male salmonid serum.　　　　　　　　　　　　　　　
Haga A, Fukada H, Fujita T, Hiramatsu N, Hara A
Enviromental Science, 8, 2, 173, 2001, [Peer-reviewed]
English, International conference proceedings

Simple and sensitive detection of vitellogenin receptor(s) in Sakhalin taimen (Hucho perryi).
Naoshi Hiramatsu, Haruhisa Fukada, Craig V. Sullivan, Akihiko Hara
Bull. Fac. Fish. Hokkaido Univ., 52, 1, 5, 9, 2001, [Peer-reviewed]

Changes of serum growth hormone and virellogenin levels during vitellogenesis in female masu salmon Oncorhynchus masou
H Fukada, N Hiramatsu, M Kitamura, M Shimizu, A Hara
FISHERIES SCIENCE, 66, 4, 789, 791, Aug. 2000, [Peer-reviewed]
English, Scientific journal

Development of a sensitive luminometric immunoassay for determining baseline seasonal changes in serum vitellogenin levels in male flounder (Pleuronectes yokohamae)
Hiroki Bessho, Sunao Iwakami, Naoshi Hiramatsu, Akihiko Hara, Shinya Hashimoto
International Journal of Environmental Analytical Chemistry, 76, 3, 155, 166, Gordon & Breach Science Publ Inc, 2000, [Peer-reviewed]
English, Scientific journal

Serum levels of precursors to vitelline envelop proteins (choriogenins) in Sakhalin taimen after treatment with estrogen and during oocyte growth.
Munetaka Shimizu, Haruhisa Fukada, Toshiaki Fujita, Naoshi Hiramatsu, Akihiko Hara
Journal of Fish Biology, 57, 170, 181, 2000, [Peer-reviewed]

Changes in serum choriogenin and vitellogenin levels in masu salmon (Oncorhynchus masou) during sexual maturation and after estrogen treatment.　　　　　　　　　　　　　　　
Fujita T, Hiramatsu N, Shimizu M, Sullivan CV, Hara A
Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, 314, 1999, [Peer-reviewed]
English

Changes of serum growth hormone and vitellogenin levels in chtthroat trout during sexual maturation.　　　　　　　　　　　　　　　
Fukada H, Hiramatsu N, Shimizu M, Sullivan CV, Hara A
Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, 314, 1999, [Peer-reviewed]
English

Purification of Immunoglobulin M (IgM) in Serum of Japanese Flounder (Paralichthys olivaceus)
NISHIDA Hiroko, ENOKIDA Tsuyoshi, HIRAMATUS Naoshi, HARA Akihiko, YOSHIMIZU Mamoru
Bulletin of the Faculty of Fisheries, Hokkaido University, 49, 3, 157, 164, 北海道大学, Dec. 1998, [Peer-reviewed]
Japanese

Immunochemical detection, estrogen induction and occurrence in serum of vitelline envelope-related proteins of Sakhalin Taimen Hucho perryi
M Shimizu, T Fujita, N Hiramatsu, A Hara
FISHERIES SCIENCE, 64, 4, 600, 605, Aug. 1998, [Peer-reviewed]
English, Scientific journal

Chemiluminescent Immunoassay (CLIA) for Salmon Growth Hormone (GH)
Haruhisa Fukada, Naoshi Hiramatsu, Makiko Kitamura, Hitoshi Chiba, Akihiko Hara
Luminescence, 12, 6, 271, 275, Nov. 1997, [Peer-reviewed]
English, Scientific journal

Development of an ELISA for chum salmon (Oncorhynchus keta) growth hormone
H Fukada, N Hiramatsu, K Gen, A Hara
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 117, 3, 387, 392, Jul. 1997, [Peer-reviewed]
English, Scientific journal

アメマスSalvelinus leucomaenisビテロゲニンの卵黄蛋白質への特異的分解
平松尚志, 原彰彦
日本水産学会誌, 63, 5, 701, 708, 1997, [Peer-reviewed]

Simple Purification of Growth Hormone from Chum Salmon(Oncorhynchus keta).
深田陽久, 玄浩一郎, 平松尚志, 浦和寛, 原彰彦
北海道大学水産学部研究い報, 47, 2/3, 31, 39, 北海道大学, Dec. 1996
Japanese

クロソイ(Sebastes schlegelii)雄尿に含まれるリポカリンタンパク質のアンドロゲンによる産生制御について
山口燿, 永田淳, 川崎琢真, 東藤孝, 平松尚志, 日本水産学会大会講演要旨集(CD-ROM), 2023, 2023

クロソイ(Sebastes schlegelii)雄尿タンパク質の同定:Three-finger proteinファミリーに属する新規タンパク質
山口燿, 足達凛太郎, 南宮眞, 川崎琢真, 東藤孝, 平松尚志, 日本水産学会大会講演要旨集(CD-ROM), 2023, 2023

オミクス解析並びに免疫生化学的手法を用いたクロソイSebastes schlegelii交尾期雄尿タンパク質の同定
山口燿, 芝竜太郎, 川崎琢真, 原彰彦, 東藤孝, 平松尚志, 日本水産学会大会講演要旨集(CD-ROM), 2021, 2021

クロソイ養殖の効率化に向けた補償成長に関する給餌試験
芝竜太郎, 山口燿, 竹内真論, 木村和世, 平井輝孝, 川崎琢真, 清水宗敬, 東藤孝, 平松尚志, 水産増殖, 69, 4, 2021

２つの海に面する八雲：日本海と太平洋の海の生物・環境を比べてみよう 報告書　　　　　　　　　　　　　　　
平松尚志, 海の宝アカデミックコンテスト 2019 ・ブルーオーシャン, Jul. 2019
Japanese

カットスロートトラウト肝臓で発現するエストロジェン応答性遺伝子のプロモーター解析
永田淳, 莚平裕次, 西宮攻, 藤田敏明, 平松尚志, 原彰彦, 東藤孝, 日本水産学会大会講演要旨集, 2019, 30, 26 Mar. 2019
Japanese

生物情報科学によるメバル属魚類の増養殖関連有用マーカーの探索　　　　　　　　　　　　　　　
平松 尚志, H30年度 南北海道学術振興財団助成事業報告書, 2019
Japanese, Technical report

カットスロートトラウト初代培養肝細胞を用いたエストラジオール‐17β応答性遺伝子の発現解析
永田淳, 莚平裕次, 西宮攻, 藤田敏明, 平松尚志, 原彰彦, 東藤孝, 日本水産学会大会講演要旨集, 2018, 13, 26 Mar. 2018
Japanese

魚の卵を科学する！魚の血液検査でオス・メスが分かる？　　　　　　　　　　　　　　　
平松 尚志, H29年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング サイエンスカフェ, 29 Jul. 2017
Japanese, Lecture materials

サクラマスインスリン様成長因子結合蛋白‐1の機能的組換え蛋白の作製
田中英絵, 中野裕介, 水田紘子, 平松尚志, 清水宗敬, 日本水産学会大会講演要旨集, 2017, 170, 26 Mar. 2017
Japanese

カットスロートトラウト雌の肝臓におけるエストラジオール‐17β応答性遺伝子の生殖周期に伴う発現変化
永田淳, 莚平裕次, 西宮攻, 平松尚志, 原彰彦, 東藤孝, 日本水産学会大会講演要旨集, 2017, 16, 26 Mar. 2017
Japanese

カレイ類受精卵及びシシャモ仔魚に対する種特異抗体の作製　　　　　　　　　　　　　　　
平松 尚志, H28年度北水協会 水産研究助成事業報告, 2017
Japanese, Technical report

メダカにおける低密度リポ蛋白質受容体遺伝子ノックアウト:CRISPR/Cas9による欠損導入初世代の作出
小山海斗, JIN Namgung, 勘林優樹, 永田淳, 莚平裕次, 吉田達也, 東藤孝, 平松尚志, 日本水産学会北海道支部大会講演要旨集, 2017, 25, 2017
Japanese

遺伝子導入メダカ初世代における組換え蛋白質の発現:pT2/TransposaseシステムとpCAGGS/I‐SceIシステムの比較
塚原杏奈, 吉田達哉, 莚平裕次, 東藤孝, 平松尚志, 日本水産学会北海道支部大会講演要旨集, 2017, 26, 2017
Japanese

ホールマウント免疫染色法による3種カレイ受精卵の種判別
笠井慶, 永田淳, 峯野博和, 藤崎雄大, 莚平裕次, 莚平裕次, 武田康孝, 藤田敏明, 東藤孝, 原彰彦, 平松尚志, 日本水産学会北海道支部大会講演要旨集, 2017, 23, 2017
Japanese

GnRH投与によるエゾメバル雌の性成熟促進
紫藤勇磨, 川崎琢真, 莚平裕次, 平松尚志, 東藤孝, 日本水産学会北海道支部大会講演要旨集, 2016, 51, 22 Oct. 2016
Japanese

カットスロートトラウト(Oncorhynchus clarki)における2型ビテロジェニンプロモーターの性状解析
莚平裕次, 西宮攻, 永田淳, 東藤孝, 原彰彦, 平松尚志, 日本水産学会大会講演要旨集, 2016, 14, 26 Mar. 2016
Japanese

カレイ類受精卵及びシシャモ仔魚に対する種特異抗体の作製　　　　　　　　　　　　　　　
平松 尚志, H27年度 北水協会水産研究助成事業報告, 15, 23, 2016
Japanese, Technical report

エゾメバルの卵巣発達に伴う3型ビテロジェニン遺伝子発現量の変化
和田怜, 西宮攻, 横野孝典, 莚平裕次, 東藤孝, 川崎琢真, 松原孝博, 澤口小有美, 原彰彦, 平松尚志, 日本水産学会大会講演要旨集, 2015, 215, 27 Mar. 2015
Japanese

カットスロートトラウト(Oncorhynchus clarki)における血漿リポ蛋白質の分離とアポリポ蛋白質Eの検出
莚平裕次, 竹下眞広, 東藤孝, 原彰彦, 平松尚志, 日本水産学会大会講演要旨集, 2014, 178, 27 Mar. 2014
Japanese

サケ科魚類のビテロジェニンに結合するリポ蛋白質受容体の探索と性状解析
莚平裕次, 水田紘子, 羅ぶん妹, 東藤孝, 原彰彦, 平松尚志, 日本水産学会大会講演要旨集, 2013, 39, 26 Mar. 2013
Japanese

cDNA cloning of pregnancy-associated glycoprotein striped dolphin
MORITA Yuka, HIRAMATSU Naoshi, IWASAKI Toshihide, TODO Takashi, HARA Akihiko, The Journal of Reproduction and Development Supplement, 104, 0, 1066, 1066, 2011
【目的】Pregnancy-associated glycoprotein (PAG)は胎盤で発現する妊娠関連タンパク質であり，妊娠母体の血中に出現することから，その検出や動態は数種の陸生哺乳類において妊娠の診断やモニタリング等に利用されている。本研究は鯨類における繁殖生理機構の一端を解明し，得られた知見を繁殖管理へ応用することを目的としてスジイルカPAG cDNAのクローニングを行った。【方法】 試料には和歌山県太地町のイルカ漁にて捕獲されたスジイルカを用いた。妊娠初期から後期のスジイルカ4個体から得た胎盤をプールしRNAを抽出後，cDNAを合成した。他の陸上哺乳類PAGの配列を元に設計したdegenerateプライマーと胎盤cDNAをPCRに供し，得られたPAG様PCR産物の配列を決定した。さらに5'および3'RACE法を用いてPAGのcDNAクローニングを行い，全一次構造の解析を試みた。【結果】スジイルカ胎盤組織より PAG のクローニングを行った結果，6 種類のPAG cDNA (pag1～6) が確認され，それらは397個（pag1,2）および404個（pag3～6）のアミノ酸翻訳領域から構成されていた。同アミノ酸配列は他の陸上哺乳類 pag と高い相同性を示し，本種の PAG をコードしていると考えられた。スジイルカpag配列には3～5個のN-glycosylationサイトおよびアスパラギン酸プロテアーゼファミリーに特徴的なドメインが含まれていた。またアスパラギン酸プロテアーゼ活性に必要なcatalytic モチーフを含んでいたことより，構造的には同活性を持ち得る可能性が示唆された。各pag cDNAの塩基配列および演繹アミノ酸配列は約90%以上の相同性を示したが，系統樹を作成したところ，2つのクラスターに分類された。さらに本種pag配列は同モチーフを有するウマおよびブタpag配列に最も近いクレードを形成した。以上本研究によりスジイルカのPAGが遺伝子レベルで同定され，鯨類で初めて詳細な性状が明らかとなった。, THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT, Japanese

カットスロートトラウト(Oncorhynchus clarki)における2型ビテロジェニン遺伝子の発現解析
莚平裕次, 水田紘子, 羅ぶんしょ, 澤口小有美, 松原孝博, 平松尚志, 東藤孝, 原彰彦, 日本水産学会北海道支部大会講演要旨集, 2011, 28, 2011
Japanese

Development of Evaluation Systems for the Detection of Estrogenic Endocrine Disrupting Chemicals (EDCs) in Aquatic Environments using Estrogen-inducible Biomarkers　　　　　　　　　　　　　　　
Naoshi Hiramatsu, Wenshu Luo, Lei Hong, Kiyoshi Soyano, Junya Aoki, Haruna Amano, Toshiaki Fujita, Takashi Todo, Takahiro Matsubara, Akihiko Hara, National Taiwan Museum Special Publication, 14, 49, 56, 2010

Purification and characterization of lipovitellin from Pacific saury Cololabis saira
AMANO HARUNA, KITAMURA MAKIKO, FUJITA TOSHIAKI, HIRAMATSU NAOSHI, TODO TAKASHI, SUYAMA SATOSHI, HARA AKIHIKO, Fisheries science : FS, 74, 4, 830, 836, 01 Aug. 2008
English

クロマグロ高度化―奄美栽培漁業センターにおける養成3歳魚雌クロマグロの繁殖特性
玄浩一郎, 二階堂英城, 香川浩彦, 松原孝博, 澤口小有美, 東藤孝, 平松尚志, 原彰彦, 武部文行, 井手健太郎, 西明文, 塩澤聡, 升間主計, 日本水産学会大会講演要旨集, 2008, 238, 27 Mar. 2008
Japanese

クロマグロ卵膜蛋白遺伝子の検出
久田剛輝, 藤田敏明, 玄浩一郎, 二階堂英城, 升間主計, 松原孝博, 東藤孝, 平松尚志, 原彰彦, 日本水産学会大会講演要旨集, 2008, 224, 27 Mar. 2008
Japanese

魚類ビテロジェニンの生体指標蛋白質としての利用　　　　　　　　　　　　　　　
平松 尚志, 天野春菜, 藤田敏明, 松原孝博, Craig V. Sullivan, 征矢野清, 原彰彦, 拠点来額方式による日韓水産学術交流 平成18年度事業報告書, 199, 202, Mar. 2006
Japanese, Meeting report

North Carolina State University, College of Agriculture and Life Science, Department of Zoology
平松 尚志, 日本比較内分泌学会ニュース = Newsletter of Japan Society for Comparative Endocrinology, 117, 28, 29, 01 May 2005
Japanese

Vitellogenesis in aquatic animals (Proceedings of International Commemorative Symposium--70th Anniversary of The Japanese Society of Fisheries Science)
HIRAMATSU N., Fisheries Science, 68, 694, 699, Nov. 2002
Japanese Society of Fisheries Science, English

卵黄蛋白前駆物質（ビテロジェニン）を指標にした成熟度判定手法の開発　　　　　　　　　　　　　　　
原彰彦, 平松尚志, 平成8年度新品種作出基礎技術開発事業研究成果の概要, 274, 297, 1998
Japanese, Technical report

Limited proteolysis of vitellogenin and its derived yolk proteins in salmonids
N Hiramatsu, N Ichikawa, K Hosokawa, A Hara, ADVANCES IN COMPARATIVE ENDOCRINOLOGY, TOMES 1 AND 2, 1481, 1486, 1997
English

卵黄蛋白前駆物質（ビテロジェニン）を指標にした成熟度判定手法の開発　　　　　　　　　　　　　　　
原彰彦, 平松尚志, 北村真紀子, 平成7年度新品種作出基礎技術開発事業研究成果の概要, 180, 192, 1996
Japanese, Technical report

卵黄蛋白前駆物質（ビテロジェニン）を指標にした成熟度判定手法の開発　　　　　　　　　　　　　　　
原彰彦, 平松尚志, 清水宗敬, 平成6年度新品種作出基礎技術開発事業研究成果の概要, 192, 203, 1995
Japanese, Technical report

Three egg yolk proteins are derived from salmonid vitellogenin　　　　　　　　　　　　　　　
In proceedings of the fifth International Symposium on the reproductive physiology, 364, 1995

卵黄蛋白前駆物質（ビテロジェニン）を指標にした成熟度判定手法の開発　　　　　　　　　　　　　　　
原彰彦, 平松尚志, 平成5年度新品種作出基礎技術開発事業研究成果の概要, 205, 213, 1994
Japanese, Technical report

卵黄蛋白前駆物質（ビテロジェニン）を指標にした成熟度判定手法の開発　　　　　　　　　　　　　　　
原彰彦, 平松尚志, 稲場琴美, 平成4年度新品種作出基礎技術開発事業研究成果の概要, 164, 178, 1993
Japanese, Technical report

道南おさかな図鑑
魚に魅せられた北大の研究者たち, イトウ―ペリー提督も見た幻の巨大魚
海文堂出版, Oct. 2023, 9784303800116, 97p, Japanese, [Joint work]

Vitellogenin as a biomarker for endocrine disruption: molecular and biochemical considerations. In: Biochemical and Molecular Biology of Fishes, pp.431-472, Vol. 6 Environmental Toxicology (T.W. Moon and T.P. Mommsen, editors).　　　　　　　　　　　　　　　
Elsevier Science BV, Amsterdam,, 2005

Vitellogenin as a biomarker for endocrine disruption: molecular and biochemical considerations. In: Biochemical and Molecular Biology of Fishes　　　　　　　　　　　　　　　
Elsevier Science BV, Amsterdam, 2005

胎生メバル類の雄尿タンパク質:蛇毒タンパク質(Three-finger toxins)と類似した新規タンパク質の同定と発現解析　　　　　　　　　　　　　　　
山口燿, 天谷貴史, 征矢野清, 南宮眞, 川崎琢真, 東藤孝, 平松尚志
日本水産学会大会講演要旨集(CD-ROM), 2025
2025 - 2025

道産魚粉配合飼料並びに同カゼイン代替飼料を用いたニジマス給餌試験　　　　　　　　　　　　　　　
佐藤龍一, 中村太祐, 菊池静, 木村克也, 大石岳人, 深田陽久, 東藤孝, 平松尚志
日本水産学会大会講演要旨集(CD-ROM), 2025
2025 - 2025

魚類卵母細胞への物質輸送システムの開発:モデル輸送体の生体内発現・生産・輸送性状の検証　　　　　　　　　　　　　　　
野村季里, 水野雄太, 川上浩一, 東藤孝, 平松尚志
日本水産学会大会講演要旨集(CD-ROM), 2025
2025 - 2025

Introduction of a possible new local species for salmon aquaculture “Itou”=“Japanese huchen”　　　　　　　　　　　　　　　
Naoshi Hiramatsu
Hokkaido Innovation Week, 29 Jan. 2024, English

Identification of urinary proteins of Sebastes rockfish: novel proteins in lipocalin family and three-finger protein family　　　　　　　　　　　　　　　
Yo Yamaguchi, Jin Namgung, Jun Nagata, Takuma Kawasaki, Akihiko Hara, Takashi Todo, Naoshi Hiramatsu
The 17th International Meeting on Reproductive Biology of Aquatic Animals, 09 Oct. 2023, English, Oral presentation
08 Oct. 2023 - 10 Oct. 2023

Androgen signaling is responsible for the renal synthesis of the urinary lipocalin protein in rockfish　　　　　　　　　　　　　　　
Yo Yamaguchi, Jun Nagata, Takuma Kawasaki, Akihiko Hara, Takashi Todo, Naoshi Hiramatsu
The 17th International Meeting on Reproductive Biology of Aquatic Animals, 09 Oct. 2023, English, Poster presentation
08 Oct. 2023 - 10 Oct. 2023

Increasing efficiency of aquaculture production by producing an all-female mono-sex population in black rockfish (Sebastes schlegelii)　　　　　　　　　　　　　　　
Mutea Fridah Gacheri, Yo Yamaguchi, Takuma, Kawasaki, Takashi Todo, Naoshi Hiramatsu
海洋研究センター成果報告会, 30 Jun. 2023, English, Poster presentation
30 Jun. 2023 - 30 Jun. 2023

メバル類のオスの成熟度を推定できる新たなバイオマーカー:リポカリン様タンパク質の測定系確立とオス成熟度指標としての利用可能性　　　　　　　　　　　　　　　
山口 燿, 川崎 琢真, 原 彰彦, 東藤 孝, 平松 尚志
海洋センター成果報告会, 30 Jun. 2023

Development of enzyme-linked immunosorbent assay (ELISA) for serum lipocalin-like protein, a potential novel biomarker relating to the male gonadal development, in Sebastes rockfish　　　　　　　　　　　　　　　
Yo Yamaguchi, Takuma Kawasaki, Akihiro Hara, Takashi Todo, Naoshi Hiramatsu
12th International Symposium on Reproductive Physiology of Fish, May 2023, English, Poster presentation
15 May 2023 - 19 May 2023

クロソイ(Sebastes schlegelii)雄尿に含まれるリポカリンタンパク質のアンドロゲンによる産生制御について　　　　　　　　　　　　　　　
山口燿, 永田淳, 川崎琢真, 東藤孝, 平松尚志
日本水産学会大会講演要旨集(CD-ROM), 2023
2023 - 2023

クロソイ(Sebastes schlegelii)雄尿タンパク質の同定:Three-finger proteinファミリーに属する新規タンパク質　　　　　　　　　　　　　　　
山口燿, 足達凛太郎, 南宮眞, 川崎琢真, 東藤孝, 平松尚志
日本水産学会大会講演要旨集(CD-ROM), 2023
2023 - 2023

不妊体ニジマスの魚卵アレルギーフリー食材としての有用性評価　　　　　　　　　　　　　　　
渡辺彩希, 石田慎太郎, 笹岡友季穂, 清水裕, 東藤孝, 平松尚志, 趙佳賢, 佐伯宏樹
日本水産学会大会講演要旨集(CD-ROM), 2022
2022 - 2022

オミクス解析並びに免疫生化学的手法を用いたクロソイSebastes schlegelii交尾期雄尿タンパク質の同定　　　　　　　　　　　　　　　
山口燿, 芝竜太郎, 川崎琢真, 原彰彦, 東藤孝, 平松尚志
日本水産学会大会講演要旨集(CD-ROM), 2021
2021 - 2021

メダカ卵への物質輸送システム開発における同種主要ビテロジェニンAa1プロモーターの利用　　　　　　　　　　　　　　　
小島哲也, 南宮眞, 永井優里, 永田淳, 莚平裕次, 川上浩一, 東藤孝, 平松尚志
日本水産学会大会講演要旨集(CD-ROM), 2021
2021 - 2021

Multiple vitellogenins in Medaka (Oryzias latipes): Characterization of the promoters and yolk derivatives
Kojima T., Namgung J, Nagata T., Mushirobira Y., Todo T., Hara A., Reading B.J., Hiramatsu N.
The 16th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 18 Oct. 2019, English, Poster presentation
17 Oct. 2019 - 19 Oct. 2019, 12411170

Impact of environmental estrogens on vitellogenin and hybrid striped bass reproduction (Vitellogenesis in Fishes)　　　　　　　　　　　　　　　
Reading, B.J., Kowalchyk, C., Andersen, L.K., Fischer, J., Mushirobira, Y., Todo, T., and Hiramatsu, N.
EDC-NC: Endocrine Disruption Special Interest Group of the Center for Human Health and the Environment, 05 Apr. 2019, English, Oral presentation
05 Apr. 2019 - 05 Apr. 2019

カットスロートトラウト肝臓で発現するエストロジェン応答性遺伝子のプロモーター解析
永田淳, 莚平裕次, 西宮攻, 藤田敏明, 平松尚志, 原彰彦, 東藤孝
日本水産学会大会講演要旨集, 26 Mar. 2019, Japanese

VITELLOGENESIS IN FISHES　　　　　　　　　　　　　　　
Benjamin Reading, Linnea Andersen, Justin Schilling, Yuji Mushirobira, Takashi Todo, Naoshi Hiramatsu
AQUACULTURE 2019, 07 Mar. 2019, English, Oral presentation
[International presentation]

The in vivo effects of estradiol and 11-ketotestosterone on vitellogenin physiology in shortfinned eel, Anguilla australis　　　　　　　　　　　　　　　
Thomson-Laing, G, Samsteegt, E.L, Nagata, J, Ijiri, S, Adachi, S, Todo, T, Hiramatsu, N, Lokman, P.M
11th International Symposium on Reproductive Physiology of Fish, 03 Oct. 2018, English, Oral presentation
[International presentation]

Mutagenesis of a major vitellogenin receptor gene with eight ligand binding repeats in Medaka using CRISPR/Cas9 system　　　　　　　　　　　　　　　
Namgung J, Oyama K, Mizuta H, Todo T, Hiramatsu N
The 15th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 31 Aug. 2018, English, Oral presentation
[International presentation]

Comparison of growth and muscle properties among black rockfish, fox jacopever, and the hybrid　　　　　　　　　　　　　　　
Takeuchi M, Kawasaki T, Todo T, Hiramatsu N
The 15th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 31 Aug. 2018, English, Poster presentation
[International presentation]

Determination of efficient microsatellite markers for parentage diagnosis in viviparous rockfish　　　　　　　　　　　　　　　
Numayama A, Kawasaki T, Todo T, Hiramastu N
The 15th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 31 Aug. 2018, English, Poster presentation
[International presentation]

Measurement of multiple vitellogenins in marbled sole, Pleunectes yokohamae, using type-specific enzyme-linked immunosorbent assays　　　　　　　　　　　　　　　
Amano H, Uno S, Koyama J, Hiramatsu N, Todo T, Hara A
The 15th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 31 Aug. 2018, English, Poster presentation
[International presentation]

Expression of estrogen responsive genes in primary cultured hepatocyte of cutthroat trout, Oncorhynchus clarki　　　　　　　　　　　　　　　
Nagata J, Mushirobira Y, Nishimiya O, Fujita T, Hiramastsu N, Hara A, Todo T
The 15th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 31 Aug. 2018, English, Poster presentation
[International presentation]

カットスロートトラウト初代培養肝細胞を用いたエストラジオール‐17β応答性遺伝子の発現解析
永田淳, 莚平裕次, 西宮攻, 藤田敏明, 平松尚志, 原彰彦, 東藤孝
日本水産学会大会講演要旨集, 26 Mar. 2018, Japanese

北方性ソイ・メバル類の増養殖に向けた基礎および応用研究　　　　　　　　　　　　　　　
柴田侑人, 平松尚志, 東藤孝
H29年度函館市国際水産・海洋総合研究センター・マリンサロン, 26 Jan. 2018, Japanese, Poster presentation
[Domestic Conference]

函館市国際水産・海洋総合研究センターにおける海洋教育研究活動の実践　　　　　　　　　　　　　　　
高原英生, 安部智貴, 平松尚志, 宮下和士
2017年度日本海洋学学会秋季大会、海洋教育特別ポスターイベント：海洋教育・アウトリーチ活動の実践と課題, 14 Oct. 2017, Japanese, Poster presentation
[Domestic Conference]

Changes in mRNA levels of estrogen responsive genes in the liver of cutthroat trout following estradiol-17beta injection.　　　　　　　　　　　　　　　
Nagata J, Mushirobira Y, Hiramatsu N, Hara A, Todo T
The 14th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea,, 01 Sep. 2017, English, Oral presentation
[International presentation]

カットスロートトラウト雌の肝臓におけるエストラジオール‐17β応答性遺伝子の生殖周期に伴う発現変化
永田淳, 莚平裕次, 西宮攻, 平松尚志, 原彰彦, 東藤孝
日本水産学会大会講演要旨集, 26 Mar. 2017, Japanese

サクラマスインスリン様成長因子結合蛋白‐1の機能的組換え蛋白の作製
田中英絵, 中野裕介, 水田紘子, 平松尚志, 清水宗敬
日本水産学会大会講演要旨集, 26 Mar. 2017, Japanese

クロソイとキツネメバルの天然妊娠魚における雄の交尾尾数の解析　　　　　　　　　　　　　　　
村山大樹, 川崎琢磨, 東藤孝, 平松尚志, 原彰彦
平成28年度函館国際水産・海洋都市構想シンポジウム・海洋研究センター研究発表会, 08 Mar. 2017, Japanese, Poster presentation
[Domestic Conference]

GnRH投与によるエゾメバル雌の性成熟促進　　　　　　　　　　　　　　　
紫藤勇磨, 川崎琢磨, 村山大樹, 猪股勇斗, 石毛友里絵, 平松尚志, 東藤孝
平成28年度函館国際水産・海洋都市構想シンポジウム・海洋研究センター研究発表会, 08 Mar. 2017, Japanese, Poster presentation
[Domestic Conference]

メダカにおけるLR8型ビテロジェニン受容体遺伝子のノックアウト:CRISPR/Cas9による変異導入初世代の作出
JIN Namgung, 小山海斗, 勘林優樹, 水田紘子, 廣島美枝, YEO In‐Kyu, 東藤孝, 平松尚志
日本水産学会北海道支部大会講演要旨集, 2017, Japanese

ホールマウント免疫染色法による3種カレイ受精卵の種判別
笠井慶, 永田淳, 峯野博和, 藤崎雄大, 莚平裕次, 莚平裕次, 武田康孝, 藤田敏明, 東藤孝, 原彰彦, 平松尚志
日本水産学会北海道支部大会講演要旨集, 2017, Japanese

遺伝子導入メダカ初世代における組換え蛋白質の発現:pT2/TransposaseシステムとpCAGGS/I‐SceIシステムの比較
塚原杏奈, 吉田達哉, 莚平裕次, 東藤孝, 平松尚志
日本水産学会北海道支部大会講演要旨集, 2017, Japanese

メダカにおける低密度リポ蛋白質受容体遺伝子ノックアウト:CRISPR/Cas9による欠損導入初世代の作出
小山海斗, JIN Namgung, 勘林優樹, 永田淳, 莚平裕次, 吉田達也, 東藤孝, 平松尚志
日本水産学会北海道支部大会講演要旨集, 2017, Japanese

Differential responses of oogenesis-related genes to estradiol-17β in the liver of cutthroat trout, (Onchorhychus clarki).　　　　　　　　　　　　　　　
Nagata, J, Mushirobira, Y, Hiramatsu, N, Hara, A, Todo, T
The 13th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 29 Nov. 2016, English, Oral presentation
[International presentation]

Expression profiles of dual vitellogenin genes in the cutthroat trout (Onchorhychus clarki) following estradiol-17β administration.　　　　　　　　　　　　　　　
Mushirobira,Y, Nagata, J, Todo, T, Hara, A, Hiramatsu, N
The 13th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 29 Nov. 2016, English, Poster presentation
[International presentation]

GnRH投与によるエゾメバル雌の性成熟促進
紫藤勇磨, 川崎琢真, 莚平裕次, 平松尚志, 東藤孝
日本水産学会北海道支部大会講演要旨集, 22 Oct. 2016, Japanese

魚の卵を科学する～基礎から応用への共同研究の可能性　　　　　　　　　　　　　　　
平松 尚志
第一回食科学プラットフォーム「水産ブロック」意見交換会, 16 Sep. 2016, Japanese, Public discourse
[Domestic Conference]

魚の卵を科学する　　　　　　　　　　　　　　　
平松 尚志
平成28年度（第30回）北海道大学水産学部公開講座「弁天町発！！水産・海洋研究の最前線」, 30 Jul. 2016, Japanese, Public discourse
[Domestic Conference]

カットスロートトラウト(Oncorhynchus clarki)における2型ビテロジェニンプロモーターの性状解析
莚平裕次, 西宮攻, 永田淳, 東藤孝, 原彰彦, 平松尚志
日本水産学会大会講演要旨集, 26 Mar. 2016, Japanese

Molecular mechanisms underlying yolk formation in fish: how to make the tailor-made yolk?　　　　　　　　　　　　　　　
Hiramatsu, N, Todo, T
The 2nd Joint Symposium of HU/NTOU & 1st International Conference on Quaternary and Future Earth, 08 Jan. 2016, English, Invited oral presentation
[International presentation]

Oogenesis in fish: what makes fish eggs ? Current understanding of yolk formation in fish　　　　　　　　　　　　　　　
HIRAMATSU Naoshi
Hokkaido University (HU) Learning Satellite “Fish ‘n Czech” 2015, 15 Sep. 2015, English, Public discourse
[International presentation]

エゾメバルの卵巣発達に伴う3型ビテロジェニン遺伝子発現量の変化
和田怜, 西宮攻, 横野孝典, 莚平裕次, 東藤孝, 川崎琢真, 松原孝博, 澤口小有美, 原彰彦, 平松尚志
日本水産学会大会講演要旨集, 27 Mar. 2015, Japanese

Analysis of lipid droplet formation in oocytes of the cutthroat trout, Oncorhynchus clarki in vitro　　　　　　　　　　　　　　　
Nagata, J, Mushirobira, Y, Hiramatsu, N, Hara, A, Todo, T
The 12th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 2015, English, Poster presentation
[International presentation]

Vitellogenesis in white perch (Morone americana): Multiple vitellogenins and their receptors　　　　　　　　　　　　　　　
Schilling, J, Hiramatsu, N, Daniels, H.V, Reading, B.J
In: abstract of Aquaculture America 2015 (https://www.was.org/meetings/ShowAbstract.aspx?Id=34659), 2015, English, Oral presentation

Molecular characterization of dual vitellogenin gene promoters in the cutthroat trout (Oncorhynchus clarki)　　　　　　　　　　　　　　　
Mushirobira, Y, Nishimiya, O, Nagata, J, Todo, T, Hara, A, Hiramatsu, N
The 12th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 2015, English, Oral presentation
[International presentation]

エゾメバル(Sebastes taczanowski)における生殖腺刺激ホルモン各サブユニットのcDNAクローニングと発現解析
横野孝典, 西宮攻, 川崎琢真, 平松尚志, 東藤孝
日本水産学会北海道支部大会講演要旨集, 2015, Japanese

免疫染色法によるシシャモ仔稚魚の判別技術開発
川崎琢真, 清水洋平, 石田良太郎, 中田訓彰, 平松尚志, 東藤孝
日本水産学会北海道支部大会講演要旨集, 2015, Japanese

Detection and identification of plasma apolipoproteins using liquid chromatography-tandem mass spectrometry in cutthroat trout, Oncorhynchus clarki　　　　　　　　　　　　　　　
Mushirobira, Y, Todo, T, Hara, A, Hiramatsu N
The 11th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 06 Dec. 2014, English, Poster presentation
[International presentation]

Sexual maturation of the white-edged rockfish (Sebastes taczanowskii): effects of photothermal manipulation on the gonadal growth and maturation　　　　　　　　　　　　　　　
和田怜, 水田紘子, 川崎琢磨真, 高畠信一, 原彰彦, 東藤孝, 平松尚志
食とバイオ国際交流シンポジウム, 03 Jun. 2014, English, Poster presentation
[Domestic Conference]

エゾメバル雄における性成熟誘導と人工授精に関する基礎研究　　　　　　　　　　　　　　　
小渡賢太, 川崎琢真, 高畠信一, 和田怜, 西宮攻, 莚平裕次, 東藤孝, 原彰彦, 平松尚志
食とバイオ国際交流シンポジウム, 03 Jun. 2014, Japanese, Poster presentation
[Domestic Conference]

Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout　　　　　　　　　　　　　　　
Mushirobira, Y, Mizuta, H, Luo, W, Todo, T, Hara, A, Reading, B.J, Sullivan, C.V, Hara, A
10th International Symposium on Reproductive Physiology of Fish, 26 May 2014, English, Poster presentation
[International presentation]

How do eggs fat? Insights into triacylglyceride uptake in the oocytes of the shortfinned eel, Anguilla australis　　　　　　　　　　　　　　　
Damsteegt, E.L, Mizuta, H, Hiramatsu, N, Lokman, P.M
10th International Symposium on Reproductive Physiology of Fish, 26 May 2014, English, Oral presentation
[International presentation]

Very low-density lipoprotein is primary carrier of neutral lipids to ooplasm lipid droplets in teleosts　　　　　　　　　　　　　　　
Ryu, Y.W, Todo, T, Hiramatsu, N, Matsubara, T, Sullivan, C.V, Hara, A
10th International Symposium on Reproductive Physiology of Fish, 26 May 2014, English, Oral presentation
[International presentation]

Ovarian yolk formation in fishes: molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins　　　　　　　　　　　　　　　
Hiramastu, N, Todo, T, Sullivan, C.V, Reading, B.J, Matsubara, T, Ryu, Y.W, Mizuta, H, Luo, W, Nishimiya, O, Wu, M, Mushirobira, Y, Hara, A
10th International Symposium on Reproductive Physiology of Fish, 26 May 2014, English, Keynote oral presentation
[Invited], [International presentation]

魚卵アレルゲンのIgE反応性変化を調べるツールとしての抗ペプチド抗体の有用性
清水裕, 平松尚志, 佐伯宏樹
日本栄養・食糧学会大会講演要旨集, 30 Apr. 2014, Japanese

ホールマウント免疫染色法を用いた3種カレイ卵の同定
峯野博和, 南宮眞, 文惠拏, 呂寅圭, 武田康孝, 西宮攻, 鳴海一侑, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2014, Japanese

カットスロートトラウト(Oncorhynchus clarki)における血漿リポ蛋白質の分離とアポリポ蛋白質Eの検出
莚平裕次, 竹下眞広, 東藤孝, 原彰彦, 平松尚志
日本水産学会大会講演要旨集, 27 Mar. 2014, Japanese

スケトウダラ卵に含まれる食物アレルゲンの精製と性状解析
田原大貴, 清水裕, 平松尚志, 渡辺一彦, 佐伯宏樹
日本水産学会大会講演要旨集, 27 Mar. 2014, Japanese

クロソイおよびキツネメバル人工授精技術の現状と課題
川崎琢真, 清水洋平, 高畠信一, 森立成, 小渡賢太, 横野孝典, 和田怜, 平松尚志, 東藤孝
日本水産学会北海道支部大会講演要旨集, 2014, Japanese

Sexual maturation of the white-edged rockfish (Sebastes taczanowskii): effects of photothermal manipulation on the gonadal growth and maturation　　　　　　　　　　　　　　　
Wada, S, Mizuta, H, Kawasaki, T, Takabatake, S, Hara, A, Todo, T, Hiramatsu, N
The 10th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 19 Nov. 2013, English, Poster presentation
[International presentation]

Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout (Oncorhynchus clarki)　　　　　　　　　　　　　　　
Mushirobira, Y, Mizuta, H, Luo, W, Todo, T, Hara, A, Hiramatsu, N
The 10th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 19 Nov. 2013, English, Oral presentation
[International presentation]

Yolk formation in fish: Multiple vitellogenins and their receptors.　　　　　　　　　　　　　　　
HIRAMATSU, NaoshiHiramatsu, N, Mizuta, H, Luo, W, Nishimiya, O, Wu, Meiqin, Mushirobira, Y, Reading, B.J, Sullivan, C.V, Todo, T, Hara, A
Diversification in Inland Finfish Aquaculture II (DIFAII)., 25 Sep. 2013, English, Oral presentation
[International presentation]

Molecular cloning and characterization of estrogen receptors from the most primitive vertebrate, the inshore hagfish /Eptatretus burgeri/.　　　　　　　　　　　　　　　
Nishimiya, O, Katsu, Y, Inagawa, H, Hiramatsu,N, Todo T, Hara, A
17th International Congress of Comparative Endocrinology, 15 Jul. 2013, English, Poster presentation
[International presentation]

サケ科魚類のビテロジェニンに結合するリポ蛋白質受容体の探索と性状解析
莚平裕次, 水田紘子, 羅ぶん妹, 東藤孝, 原彰彦, 平松尚志
日本水産学会大会講演要旨集, 26 Mar. 2013, Japanese

カットスロートトラウト卵巣におけるクラスリン重鎖転写産物および同翻訳産物の局在に関する組織学的解析
水田紘子, 東藤孝, 原彰彦, 平松尚志
日本水産学会大会講演要旨集, 26 Mar. 2013, Japanese

ヌタウナギの2種エストロジェン受容体の機能解析
西宮攻, 勝義直, 稲川裕之, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 26 Mar. 2013, Japanese

ペプチド抗体を用いた魚卵アレルギーの魚種間交差性に関する検討
清水裕, 岸村栄毅, 平松尚志, 原彰彦, 佐伯宏樹
日本水産学会大会講演要旨集, 26 Mar. 2013, Japanese

卵生脊椎動物におけるビテロジェニン分子の成立と進化の解明　　　　　　　　　　　　　　　
西宮攻, 国弘康之, 山根広大, 平松尚志, 東藤孝, 原明彦
生命情報科学若手の会 第4回研究会, 01 Mar. 2013, Japanese, Oral presentation
[Domestic Conference]

Multiplicity of vitellogenin and their receptors in genus Morone.　　　　　　　　　　　　　　　
Sullivan CV, Reading BJ, Schlling JD, Williams VN, Glassbrook N, Hiramatsu N, Luo W, Mizuta H, Todo T, Hara A
Aquaculture 2013, 22 Feb. 2013, English, Oral presentation
[International presentation]

Dual vitellogenins in cutthroat trout (Oncorhynchus clarki): purification and changes in serum proten level during reproductive cycle.　　　　　　　　　　　　　　　
Mushirobira Y, Mizuta H, Luo W, Morita Y, Sawaguchi S, Matsubara T, Hiramatsu N, Todo T, Hara A
The 9th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 20 Dec. 2012, English, Poster presentation
[International presentation]

Molecular biological and immunochemical studies of vitellogenin in loach (Misgurnus anguillicaudatus).　　　　　　　　　　　　　　　
Wu M, Nishimiya O, Mizuta H, Hiramatsu N, Todo T, Nakamori M, Suzuki A, Soyano K, Hara A
The 9th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 20 Dec. 2012, English, Oral presentation
[International presentation]

Molecular cloning and characterization of two estrogen receptor subtypes from a hagfish Eptairetus burgeri.　　　　　　　　　　　　　　　
Nishimiya O, Inagawa H, Katsu Y, Hiramatsu N, Todo T, Hara A
The 9th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, 20 Dec. 2012, English, Oral presentation
[International presentation]

カットスロートトラウトにおける常染色体劣性高コレステロール血症原因蛋白質(ARH)のcDNAクローニング及び遺伝子発現解析
水田紘子, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 26 Mar. 2012, Japanese

サクラマスの銀化に伴うインスリン様成長因子‐Iの変化と鰓Na+,K+‐ATPaseとの関係
下村考弘, 中嶋拓郎, 堀越萌李, 飯嶋亜内, 卜部浩一, 水野伸也, 平松尚志, 原彰彦, 清水宗敬
日本水産学会大会講演要旨集, 26 Mar. 2012, Japanese

ヌタウナギの2種ビテロジェニン遺伝子および2種エストロジェン受容体遺伝子の発現解析
西宮攻, 國弘康之, 稲川裕之, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 26 Mar. 2012, Japanese

低濃度エストロジェン投与によるマコガレイ3タイプビテロジェニンの血中動態
天野春菜, 細見靖道, 森山俊介, 藤井一則, 平松尚志, 東藤孝, 原彰彦
日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨, 2012, Japanese

カットスロートトラウト卵黄におけるスカベンジャー受容体クラスＢタイプＩ遺伝子の発現解析　　　　　　　　　　　　　　　
斎藤恭一, 柳ヨンウン, 伊東優太, 平松尚志, 東藤孝, 原彰彦
第5回サケ学研究会, Dec. 2011, Japanese, Oral presentation
[Domestic Conference]

卵黄蛋白前駆物質ビテロジェニンの異種間投与とその運搬過程：イトウとゼブラフィッシュを用いたモデルについて　　　　　　　　　　　　　　　
櫻井秀之, 川北奈央子, 平松尚志, 東藤孝, 原彰彦
第5回サケ学研究会, Dec. 2011, Japanese, Oral presentation
[Domestic Conference]

サクラマスにおけるインスリン様成長因子結合蛋白―1の発現パターンと成長の関係　　　　　　　　　　　　　　　
川口航平, 下村孝弘, 中野祐介, 木村志津雄, 原彰彦, 清水宗敬
第5回サケ学研究会, Dec. 2011, Japanese, Oral presentation
[Domestic Conference]

サクラマスの銀化変態期における鰓Na+/K+-ATPase 活性とインスリン様成長因子-1　　　　　　　　　　　　　　　
下村孝弘, 中嶋拓郎, 堀越萌李, 飯嶋亜内, 卜部浩一, 水野伸也, 平松尚志, 原彰彦, 清水宗敬
第5回サケ学研究会, Dec. 2011, Japanese, Oral presentation
[Domestic Conference]

Dual vitellogenins in cutthroat trout (Oncorhynchus clarki): molecular cloning, structural characterization, and changes in hepatic expression during reproductive cycle.　　　　　　　　　　　　　　　
Mushirobira Y, Mizuta H, Luo W, Sawaguchi S, Matsubara T, Hiramatsu N, Todo T, Hara A
The 8th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Oct. 2011, English, Poster presentation
[International presentation]

抗シジミビテリン抗体を用いたヤマトシジミの雌雄判別
寺西哲夫, 水野伸也, 小出展久, 田中浩, 桑田博, 平松尚志, 足立伸次
日本水産学会大会講演要旨集, 28 Sep. 2011, Japanese

スジイルカにおけるpregnancy-associated glycoprotein cDNAのクローニング　　　　　　　　　　　　　　　
盛田祐加, 平松尚志, 岩崎俊秀, 東藤孝, 原彰彦
平成23年度繁殖生物学会, Sep. 2011, Japanese, Oral presentation
[Domestic Conference]

Novel class of ovarian liporotein receptor in cutthroat trout: Molecular cloning and expression analysis　　　　　　　　　　　　　　　
Hiramatsu N, Luo W, Mizuta H, Todo T, Reading BJ, SUllivan CV, Hara A
9th international symposium on reproductive physiology of fish, Aug. 2011, English, Oral presentation
[International presentation]

マコガレイの3タイプのビテロジェニンに対する酵素免疫測定法の確立　　　　　　　　　　　　　　　
天野春菜, 森山俊介, 平松尚志, 東藤孝, 原彰彦
第24回北里大学バイオサイエンスフォーラム, Aug. 2011, Japanese, Public discourse
[Domestic Conference]

Expression of genes involved in oocyte lipidation in cutthroat trout, Oncorhynchus clarki.　　　　　　　　　　　　　　　
Ryu Y, Tanaka R, Kasai A, Saito K, Kanno Y, Ito Y, Hiramatsu N, Todo T, Sullivan CV, Hara A
9th international symposium on reproductive physiology of fish, Aug. 2011, English, Poster presentation
[International presentation]

Molecular characterization and expression analysis of estrogen receptor and vitellogenins in inshore hagfish (Eptatretus burgeri)　　　　　　　　　　　　　　　
Nishimiya O, Kunihiro Y, Hiramatsu N, Inagawa H, Todo T, Matsubara T, Reading BJ, Sullivan CV, Hara A
9th international symposium on reproductive physiology of fish, Aug. 2011, English, Poster presentation
[International presentation]

Molecular cloning and localization of two classical ovarian lipoprotein receptors in cutthroat trout (Oncorhynchus clarki)　　　　　　　　　　　　　　　
Mizuta H, Hiramatsu N, Todo T, Ito Y, Gen K, Kazeto Y, Sullivan CV, Reading BJ, Hara A
9th international symposium on reproductive physiology of fish, Aug. 2011, English, Poster presentation
[International presentation]

マコガレイの3型ビテロジェニンに対する酵素免疫測定法の確立
天野春菜, 森山俊介, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2011, Japanese

スジイルカalpha‐fetoprotein遺伝子の発現動態
盛田祐加, 平松尚志, 藤田敏明, 天野春菜, 岩崎俊秀, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2011, Japanese

人工飼育条件下におけるヤマトシジミの成熟促進
寺西哲夫, 水野伸也, 小出展久, 守山義昭, 平松尚志, 足立伸次
日本水産学会大会講演要旨集, 27 Mar. 2011, Japanese

イトヨ腎臓におけるスピギンとアンドロジェン受容体の発現解析
瀬川卓也, 松尾昴, 長江真樹, 平松尚志, 東藤孝, 原彰彦
日本水産学会北海道支部大会講演要旨集, 2011, Japanese

カットスロートトラウト(Oncorhynchus clarki)における2型ビテロジェニン遺伝子の発現解析
莚平裕次, 水田紘子, 羅ぶんしょ, 澤口小有美, 松原孝博, 平松尚志, 東藤孝, 原彰彦
日本水産学会北海道支部大会講演要旨集, 2011, Japanese

カットスロートトラウト卵巣におけるリポ蛋白質受容体の局在解析
水田紘子, 伊東優太, 平松尚志, 東藤孝, 原彰彦
日本水産学会北海道支部大会講演要旨集, 2011, Japanese

ヌタウナギにおける2種エストロジェン受容体のcDNAクローニングおよび発現解析
西宮攻, 國弘康之, 稲川裕之, 平松尚志, 東藤孝, 原彰彦
日本水産学会北海道支部大会講演要旨集, 2011, Japanese

サクラマスにおける海水適応能の発達とインスリン様成長因子ー１の発現パターン　　　　　　　　　　　　　　　
下村孝弘, 堀越萌李, 中嶋拓郎, 飯嶋亜内, 卜部浩一, 水野伸也, 平松尚志, 原彰彦, 清水宗敬
第4回サケ学研究会, Dec. 2010, Japanese, Oral presentation
[Domestic Conference]

カットスロートトラウト卵巣におけるリポタンパクリパーゼファミリー遺伝子の発現解析　　　　　　　　　　　　　　　
柳ヨンウン, 田中莉夏子, 笠原あゆみ, 全先清博, 伊東優太, 平松尚志, 東藤孝, 原彰彦
第4回サケ学研究会, Dec. 2010, Japanese, Oral presentation
[Domestic Conference]

Molecular cloning and structural characterization of a putative estrogen receptor in inshore hagfish (Eptatretus burgeri)　　　　　　　　　　　　　　　
Nishimiya O, Kunihiro Y, Hiramatsu N, Inagawa H, Todo T, Hara A
JSPS Core University Program Seminar: Proposal to Sustainable Fisheries, Dec. 2010, English, Poster presentation
[International presentation]

Expression analysis of lipoprotein lipase family genes: involvement in processes of lipid accumulation into oocyte of cutthroat trout, Oncorhynchus clarkii　　　　　　　　　　　　　　　
Ryu YW, Tanaka R, Kasahara A, Massaki K, Ito Y, Hiramatsu N, Todo T, Hara A
JSPS Core University Program Seminar: Proposal to Sustainable Fisheries, Dec. 2010, English, Poster presentation
[International presentation]

A novel ovarian membrane receptor (LRX+1) belonging to low density lipoprotein receptor gene family: characterization of the primary structure and expression in cutthroat trout　　　　　　　　　　　　　　　
Luo W, Hiramatsu N, Todo T, Reading BJ, Sullivan CV, Hara A
JSPS Core University Program Seminar: Proposal to Sustainable Fisheries, Dec. 2010, English, Poster presentation
[International presentation]

Expression and localization of two ovarian lipoprotein receptor proteins in cutthroat trout (Oncorhynchus clarkii)　　　　　　　　　　　　　　　
Mizuta H, Hiramastu N, Todo T, Kudo H, Hara A
JSPS Core University Program Seminar: Proposal to Sustainable Fisheries, Dec. 2010, English, Poster presentation
[International presentation]

Molecular characterization of hagfish (Eptatretus burgeri) estrogen receptor (ER)　　　　　　　　　　　　　　　
Nishimiya O, Kunihiro Y, Hiramatsu N, Inagawa H, Todo T, Hara A
The 7th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Nov. 2010, English, Poster presentation
[International presentation]

Characterization of ovarian lipoprotein receptor proteins in cutthroat trout: vitellogenin receptor and low-density lipoprotein rceptor　　　　　　　　　　　　　　　
Mizuta H, Hiramatsu N, Todo T, Kudo H, Hara A
The 7th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Nov. 2010, English, Poster presentation
[International presentation]

Molecular cloning and expression analysis of a novel ovarian lipoprotein receptor in cutthroat trout (Oncorhynchus clarkii)　　　　　　　　　　　　　　　
Luo W, Hiramatsu N, Todo T, Reading BJ, Sullivan CV, Hara A
The 7th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Nov. 2010, English, Poster presentation
[International presentation]

Studies on molecular mechanisms of lipid uptake into fish oocytes: identification and expression analysis of lipoproteins lipase family genes in the ovary of cutthroat trout, Oncorhynchus clarkii　　　　　　　　　　　　　　　
Ryu YW, Tanaka R, Kasahara A, Massaki K, Ito Y, Hiramatsu N, Todo T, Hara A
The 7th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Nov. 2010, English, Poster presentation
[International presentation]

ボラを調査対象生物とした東シナ海沿岸域における環境ホルモン汚染の実態調査
板敷祥昌, WU Meiqin, ZHONG Junsheng, PARK Chang‐Beom, 青木純哉, 平松尚志, 原彰彦, 征矢野清
日本水産学会大会講演要旨集, 22 Sep. 2010, Japanese

ヤマトシジミビテリン量の測定
寺西哲夫, 水野伸也, 小出展久, 大川和之, 守山義昭, 平松尚志, 足立伸次
日本水産学会大会講演要旨集, 22 Sep. 2010, Japanese

ヤマトシジミビテリンの精製と免疫組織化学的局在
寺西哲夫, 水野伸也, 小出展久, 吉田豊, 大川和之, 守山義昭, 平松尚志, 原彰彦, 足立伸次
日本水産学会大会講演要旨集, 26 Mar. 2010, Japanese

カットスロートトラウト卵巣におけるビテロジェニン受容体蛋白質の性状解析
水田紘子, 伊東優太, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 26 Mar. 2010, Japanese

魚類の卵形成に関与する卵黄前駆物質およびその受容体に関する研究
平松尚志
日本水産学会大会講演要旨集, 26 Mar. 2010, Japanese

イルカ類の胎児および妊娠雌血清中におけるalpha‐fetoproteinの動態
盛田祐加, 平松尚志, 藤田敏明, 天野春菜, 岩崎俊秀, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 26 Mar. 2010, Japanese

ヌタウナギの2型ビテロジェニン遺伝子のプロモーター領域解析
西宮攻, 國弘康之, 天野春菜, 藤田敏明, 稲川裕之, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 26 Mar. 2010, Japanese

カットスロートトラウト卵巣におけるリポタンパクリパーゼファミリー遺伝子の発現解析
柳蓉法, 田中莉夏子, 伊東優太, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 26 Mar. 2010, Japanese

ヌタウナギのエストロジェン受容体遺伝子のクローニング
西宮攻, 國弘康之, 稲川裕之, 平松尚志, 東藤孝, 原彰彦
日本水産学会北海道支部大会講演要旨集, 2010, Japanese

Development of evaluation systems for the detection of estrogenic endocrine disrupting chemicals (EDCs) in aquatic environments using estrogen-inducible biomarkers.　　　　　　　　　　　　　　　
Hiramatsu N, Luo W, Hong L, Soyano K, Aoki J, Amano H, Fujita T, Todo T, Matsubara T, Hara A
International Symposium on Formosa Landlocked salmon and Masu Salomon, Oct. 2009, English, Invited oral presentation
[International presentation]

Purification and molecular cloning of alpha-fetoprotein in fetal striped dolphin, Stenella coeruleoalba　　　　　　　　　　　　　　　
Morita Y, Hiramatsu N, Fujita T, Amano H, Iwasaki T, Todo T, Hara A
18th Biennial confference on the Biology of Marine Mammals, Oct. 2009, English, Poster presentation
[International presentation]

Molecular cloning and expression analysis of lipoprotein lipase family genes from ovary of cutthroat trout, Oncorhynchus clarkii.　　　　　　　　　　　　　　　
Ryu YW, Tanaka R, Kasahara A, Massaki K, Ito Y, Hiramatsu N, Todo T
The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Sep. 2009, English, Poster presentation
[International presentation]

Low-density-lipoprotein receptor in cutthroat trout (Oncorhynchus clarkii): molecular cloning and experssion analysis.　　　　　　　　　　　　　　　
Ito Y, Massaki K, Ito T, Hiramatsu N, Todo T, Fujita T, Hara A
The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Sep. 2009, English, Poster presentation
[International presentation]

Estrogenic activities of coastal aquatic environments in China: evaluation using a model species, the red lip mullet (Chelon haematochelilus).　　　　　　　　　　　　　　　
Wu M, Aoki J, Hiramatsu N, Hara A, Soyano K, Zhong J
The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Sep. 2009, English, Poster presentation
[International presentation]

ヌタウナギの2型ビテロジェニン遺伝子のクローニング
國弘康之, 天野春菜, 藤田敏明, 稲川裕之, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2009, Japanese

カットスロートトラウト卵巣におけるリポタンパクリパーゼのcDNAクローニングと発現解析
東藤孝, 笠原あゆみ, 田中莉夏子, 伊東優太, 全先清博, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2009, Japanese

マコガレイの卵黄蛋白リポビテリンの検索と精製
天野春菜, 藤田敏明, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2009, Japanese

スジイルカalpha‐fetoproteinの精製と定性
盛田祐加, 平松尚志, 藤田敏明, 天野春菜, 岩崎俊秀, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2009, Japanese

カットスロートトラウト低密度リポ蛋白質受容体(LDLR)遺伝子の発現解析
伊東優太, 全先清博, 伊藤貴洋, 平松尚志, 東藤孝, 藤田敏明, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2009, Japanese

魚類のリポタンパク質と受容体に関する研究　　　　　　　　　　　　　　　
平松 尚志
平成20年度北海道海洋生物科学研究会シンポジウム, Nov. 2008, Japanese, Public discourse
[Domestic Conference]

Involvement of multiple vitellogenin-system in control and adaptation mechanism of egg buoyancy in marine teleosts.　　　　　　　　　　　　　　　
Matsubara T, Ohkubo N, Nagae M, Hiramatsu N, Amano H, Reading BJ, Sullivan CV, Hara A
World Fisheries Congress, Oct. 2008, English, Poster presentation
[International presentation]

Molecular characterization of hagfish vitellogenin　　　　　　　　　　　　　　　
Kunihiro Y, Hiramatsu N, Fujita T, Amano H, Inagawa H, Todo T, Hara A
5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Sep. 2008, English, Poster presentation
[International presentation]

Purification of putative alpha-fetoprotein in fetal serum of striped dolphin, Stenella coeruleoalba　　　　　　　　　　　　　　　
Morita Y, Hiramatsu N, Fujita T, Amano H, Iwasaki T, Todo T, Hara A
5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea, Sep. 2008, English, Poster presentation
[International presentation]

Multiple biomarkers for detecting estrogenic endocrine disruption　　　　　　　　　　　　　　　
Hiramatsu N, Hara A
International Universities Exchange Seminar Sustainability and Risk Management of Seafood and Ocean Ecosystem Conservarion, Aug. 2008, English, Oral presentation
[International presentation]

Molecular cloning and characterization of low density lipoprotein receptor in cutthroat trout (Oncorhynchus clarki)　　　　　　　　　　　　　　　
Massaki K, Hiramatsu N, Todo T, Fujita T, Sullivan CV, Hara A
Sex Determination and Gametogenesis in Fish: CUrrent status and Future Directions, May 2008, English, Poster presentation
[International presentation]

Expression of lipoprotein lipase gene in ovarian follicles of cutthroat trout (Oncorhynhus clarki) during gonadal development.　　　　　　　　　　　　　　　
Todo T, Kasahara A, Massaki K, Hiramatsu N, Sullivan CV, Hara A
Sex Determination and Gametogenesis in Fish: CUrrent status and Future Directions, May 2008, English, Poster presentation
[International presentation]

Teleost oocyte growth, vitellogenesis, and cytoplasmic maturation　　　　　　　　　　　　　　　
Sullivan CV, Reading BJ, Hiramatsu N, Todo T, Matsubara T, Sawaguchi S, Amano H, Hara A
Sex Determination and Gametogenesis in Fish: CUrrent status and Future Directions, May 2008, English, Oral presentation
[International presentation]

Yolk assembly in teleost: recent findings on the deposition of ovarian lipids and proteins　　　　　　　　　　　　　　　
Hiramatsu N, Todo T, Ito T, Massaki K, Kasahara A, Amano H, Reading BJ, Matsubara T, Sawaguchi S, Sullivan CV, Hara A
World Aquaculture 2008, May 2008, English, Poster presentation
[International presentation]

イトヨの多型ビテロジェニンの精製
高橋美咲, 天野春菜, 藤田敏明, 平松尚志, 東藤孝, 松原孝博, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2008, Japanese

クロマグロ卵膜蛋白遺伝子の検出
久田剛輝, 藤田敏明, 玄浩一郎, 二階堂英城, 升間主計, 松原孝博, 東藤孝, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2008, Japanese

カットスロートトラウト低密度リポ蛋白質受容体のcDNAクローニング
全先清博, 平松尚志, 東藤孝, 藤田敏明, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2008, Japanese

ヌタウナギのビテロジェニンならびにその関連卵黄蛋白の検索および精製
國弘康之, 天野春菜, 藤田敏明, 稲川裕之, 平松尚志, 東藤孝, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2008, Japanese

ウニの生殖細胞マーカーとしての抗Vasa抗体の作製
東藤孝, 森川遥, 武智静子, 大澤秀一, 須川祐, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2008, Japanese

カットスロートトラウト卵巣における脂肪酸輸送膜蛋白(CD36)のcDNAクローニング
伊藤貴洋, 平松尚志, 東藤孝, 藤田敏明, 千葉仁志, 原彰彦
日本水産学会大会講演要旨集, 27 Mar. 2008, Japanese

クロマグロ高度化―奄美栽培漁業センターにおける養成3歳魚雌クロマグロの繁殖特性
玄浩一郎, 二階堂英城, 香川浩彦, 松原孝博, 澤口小有美, 東藤孝, 平松尚志, 原彰彦, 武部文行, 井手健太郎, 西明文, 塩澤聡, 升間主計
日本水産学会大会講演要旨集, 27 Mar. 2008, Japanese

Purification of multiple vitellogenins in three-spined stickleback (Gasterosteus aculeatus)　　　　　　　　　　　　　　　
Takahashi M, Hiramatsu N, Fujita T, Todo T, Matsubara T, Hara A
4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals., Nov. 2007, English, Poster presentation
[International presentation]

Molecular cloning of CD36 in cuttroat trout (Oncorhynchus clarkii)　　　　　　　　　　　　　　　
Ito T, Hiramatsu N, Fujita T, Amano H, Todo T, Hara A
4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals., Nov. 2007, English, Poster presentation
[International presentation]

Purification of vitellogenin and lipovitellin-like yolk protein in hagfish (Eptatretus burgeri)　　　　　　　　　　　　　　　
Kunihiro Y, Amano H, Fujita T, Inagawa H, Hiramatsu N, Todo T, Hara A
4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals., Nov. 2007, English, Poster presentation
[International presentation]

Multiple vitellogenins and their derived yolk proteins in grey mullet (Mugil cephalus): differential proteolytic patterns during oocyte growth and maturation　　　　　　　　　　　　　　　
Amano H, Fujita T, Hiramatsu N, Matsubara T, Sullivan CV, Hara A
4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals., Nov. 2007, English, Poster presentation
[International presentation]

Monitoring of endocrine disruptant usig serum protein as bio-markers　　　　　　　　　　　　　　　
Hiramatsu N, Hara A
International Workshop on the Evaluation of the 21st COE Program, Marine Bio-manipulation Frontier for Food Production., Nov. 2007, English, Oral presentation
[International presentation]

サケ科魚類の卵黄形成―多型ビテロジェニンとコリオジェニン　　　　　　　　　　　　　　　
藤田敏明, 望月麻智子, 天野春菜, 平松尚志, 東藤孝, 原彰彦
第1回サケ学研究会, 24 Sep. 2007, Japanese, Oral presentation
[Domestic Conference]

Differential synthesis and uptake of dual vitellogenins in Japanese medaka (Oryzias latipes).　　　　　　　　　　　　　　　
Hiramastu N, Inoue M, Ideuchi H, Fujita T, Amano H, Matsubara T, Sullivan CV, Hara A
8th International Symposium on Reproductive Physiology of Fish, Jun. 2007, English, Poster presentation
[International presentation]

Controlled accumulation of multiple vitellogenins into ocytes during vitellogenesis in the barfin flounder, Verasper moseri.　　　　　　　　　　　　　　　
Sawaguchi S, Ohkubo N, Amano H, Hiramatsu N, Hara A, Sullivan CV, Matsubara T
8th International Symposium on Reproductive Physiology of Fish, Jun. 2007, English, Poster presentation
[International presentation]

Yolk precursors in white perch (Morone americana): deduced primary structures of three types of vitellogenin (vg) proteins and disparate binding of the different Vgs to multiple ovarian receptors　　　　　　　　　　　　　　　
Reading BJ, Hiramatsu N, Matsubara T, Hara A, Sullivan CV
8th International Symposium on Reproductive Physiology of Fish, Jun. 2007, English, Poster presentation
[International presentation]

Purification and classification of egg yolk proteins derived from multiple vitellogenins in grey mullet (Mugil cephalus)　　　　　　　　　　　　　　　
Amano H, Fujita T, Hiramatsu N, Matsubara T, Sullivan CV, Hara A
8th International Symposium on Reproductive Physiology of Fish, Jun. 2007, English, Oral presentation
[International presentation]

トラザメのリポビテリンおよびホスビチンの精製
山根広大, 菅原理恵子, 天野春菜, 藤田敏明, 杉下孝治, 高谷広行, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, 28 Mar. 2007, Japanese

サケ科魚類雄魚の血中ビテロジェニンおよびコリオジェニン―回遊期と遡上期での比較―
西川友典, 望月麻智子, 藤田敏明, 遊佐清明, 山本潤, 亀井佳彦, 高木省吾, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, 28 Mar. 2007, Japanese

大西洋タラの卵膜蛋白前駆物質(コリオジェニン)の精製
藤田敏明, 天野春菜, SCOTT A. P, KATSIADAKI I, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, 28 Mar. 2007, Japanese

ボラ卵黄蛋白の催熟前後における比較
天野春菜, 藤田敏明, 平松尚志, 香川浩彦, 原彰彦
日本水産学会大会講演要旨集, 28 Mar. 2007, Japanese

Multiple egg yolk precursors (vitellogenins) and egg quality in teleost fishes.　　　　　　　　　　　　　　　
Reading BJ, Hiramatsu N, Williams VN, Sullivan CV, Sawaguchi S, Matsubara T, Amano H, Hara A
Aquaculture America 2007, Feb. 2007, English, Oral presentation
[International presentation]

Development of monitoring system on endocrine disruption.　　　　　　　　　　　　　　　
Hiramatsu N, Amano H, Fujita T, Hara A
Symposium on Developing Fisheries Science in Asia., Dec. 2006, English, Oral presentation
[International presentation]

Fish vitellogenin: single versus multiple vitellogenin models.　　　　　　　　　　　　　　　
Hiramatsu N, Amano H, Fujita T, Matsubara T, Todo T, Nagae M, Soyano K, Hara A
3rd Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals., Nov. 2006, English, Oral presentation
[International presentation]

The multiple vitellogenin system of Xenotoca eiseni　　　　　　　　　　　　　　　
Sullivan CV, Williams VN, Reading BJ, Hiramatsu N, Sawaguchi S, Matsubara T, Amano H, Hara A
The III international symposium on viviparous fishes, Nov. 2006, English, Oral presentation
[International presentation]

Purification of C-type vitellogenin (VgC) in Sakhalin taimen.　　　　　　　　　　　　　　　
Mochizuki M, Amano H, Fujita T, Hiramtasu N, Hara A
The 6th Joint Seminar between Japan and Korea by Core University Program on Fisheries Sciences- Sustainability of Fisheries in Japan and Korea-, Aug. 2006, English, Poster presentation
[International presentation]

Multiple forms of vitellogenin and choriogenin in red lip mullet (Chelon haematocheilus): Immunological detection using type-specific antisera.　　　　　　　　　　　　　　　
Hong L, Hiramatsu N, Amano H, Fujita T, Hara A
The 6th Joint Seminar between Japan and Korea by Core University Program on Fisheries Sciences- Sustainability of Fisheries in Japan and Korea-, Aug. 2006, English, Poster presentation
[International presentation]

Purification and classification of egg yolk proteins in grey mullet (Mugil cephalus)　　　　　　　　　　　　　　　
Amano H, Fujita T, Hiramatsu N, Shimizu M, Sawaguchi S, Matsubara T, Kagawa H, Sullivan CV, Hara A
The 6th Joint Seminar between Japan and Korea by Core University Program on Fisheries Sciences- Sustainability of Fisheries in Japan and Korea-, Aug. 2006, English, Poster presentation
[International presentation]

魚類ビテロジェニンの生体指標蛋白質としての利用　　　　　　　　　　　　　　　
平松尚志, 天野春菜, 藤田敏明, 松原孝博, Sullivan CV, 征矢野清, 原彰彦
The 6th Joint Seminar between Japan and Korea by Core University Program on Fisheries Sciences-Sustainability of Fisheries in Japan and Korea-, Aug. 2006, English, Oral presentation
[International presentation]

Purification of multiple vitellogenins from grey mullet.　　　　　　　　　　　　　　　
Amano H, FUjita T, Hiramatsu N, Hara A
The 5th International Symposium of Industrial-Academia-Govermental Collaboration for the Establishment of Marine Production in China and Japan, Jul. 2006, English, Poster presentation
[International presentation]

ボラの3型ビテロジェニンの精製
天野春菜, 藤田敏明, 平松尚志, 沢口小有美, 松原孝博, 原彰彦
日本水産学会大会講演要旨集, 30 Mar. 2006, Japanese

ボラを用いた環境ホルモンのモニタリングー3型ビテロジェニンの精製ー　　　　　　　　　　　　　　　
天野春菜, 藤田敏明, 平松尚志, 原彰彦
21世紀COEプログラム「海洋生命統御による食糧生産の革新」都市エリア産学官連携促進事業「水産・海洋に特化したライフサイエンス領域」合同成果発表会「函館エリアにおけるライフサイエンスの最前線」, Mar. 2006, Japanese, Poster presentation
[Domestic Conference]

Molecular Characterization of three forms of vitellogenin and their yolk protein products during oovyte growth and maturation in red seabream, pagrus major　　　　　　　　　　　　　　　
Sawaguchi S, Kagawa H, Ohkubo N, Hiramtasu N, Sullivan CV, Hara A, Matsubara T
4th International Symposium of Reproductive, Genetica dn Desease Management in Aquaculture and Ocean Ranching, Aug. 2005, English, Poster presentation
[International presentation]

Multiple lipovitellins in grey mullet: N-terminal amino acid sequencing and mapping of their constituent polypeptides.　　　　　　　　　　　　　　　
Amano H, Fujita T, Hiramatsu N, Sawaguchi S, Matsubara T, Kagawa H, Sullivan CV, Hara A
4th International Symposium of Reproductive, Genetica dn Desease Management in Aquaculture and Ocean Ranching, Aug. 2005, English, Poster presentation
[International presentation]

ボラ血清中の複数ビテロジェニンの検索および精製
天野春菜, 藤田敏明, 平松尚志, YEO In‐Kyu, 香川浩彦, 原彰彦
日本水産学会大会講演要旨集, 01 Apr. 2005, Japanese

ボラビテロジェニンの性状解析　　　　　　　　　　　　　　　
天野春菜, 藤田敏明, 平松尚志, Yeo IK, 香川浩彦, 原彰彦
北海道大学市民オープンフォーラム「海・函館・北海道発わたしたちの健康と水産物の安全・安心, Mar. 2005, Japanese, Public discourse
[Domestic Conference]

Detection of three vitellogenins in serum of grey mullet.　　　　　　　　　　　　　　　
Amano H, Fujita T, Hiramatsu N, Yeo IK, Kagawa H, Sullivan CV, Hara A
1st International Symposium of "potential and perspective of Marine Bio-Manipulation"., Feb. 2005, English, Poster presentation
[International presentation]

Hybrid striped bass farming in the United States: Research and industry development. 4th International Symposium of "Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching"　　　　　　　　　　　　　　　
21st COE symposium, 2005

Hybrid striped bass farming in the United States: Research and industry development. 4th International Symposium of "Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching"　　　　　　　　　　　　　　　
21st COE symposium, 2005

Vitellogenin multiplicity and egg quality in teleost fishes　　　　　　　　　　　　　　　
Reading BJ, Hiramatsu N, Matsubara T, Hara A, Sullivan CV
134th Annual meeting of the American Fisheries Society, Aug. 2004, English, Poster presentation
[International presentation]

Immunoassays of fish vitellogenin　　　　　　　　　　　　　　　
Hara A, Fukada H, Hiramatsu N
World Aquaculture Society, Mar. 2004, English, Invited oral presentation
[International presentation]

Fish vitellogenin as a biomarker for environmental estrogens.　　　　　　　　　　　　　　　
Bilateral Seminar Italy and Japan, 2004

Multiple vitellogenins in tilapia　　　　　　　　　　　　　　　
ハワイ大学 夏期プログラム （Edwin W. Pauley Summer Program in Marine Biology), 2004

Oogenesis in teleost: Myths and Miracles: Opportunities and Applications for Basic Research　　　　　　　　　　　　　　　
ハワイ大学 夏期プログラム （Edwin W. Pauley Summer Program in Marine Biology), 2004

Oogenesis in teleost: Myths and Miracles: Opportunities and Applications for Basic Research　　　　　　　　　　　　　　　
Edwin W. Pauley Summer Program in Marine Biology, 2004

Multiple vitellogenins in tilapia　　　　　　　　　　　　　　　
Edwin W. Pauley Summer Program in Marine Biology, 2004

Fish vitellogenin as a biomarker for environmental estrogens.　　　　　　　　　　　　　　　
Bilateral Seminar Italy and Japan, 2004

Ovarian atresia in temperate basses: detection of hatching enzymes (choriolysins) in atretic ovaries　　　　　　　　　　　　　　　
Donato D, Hiramatsu N, Arey KA, Hiramatsu K, Kennedy AM, Morton CL, Hara A, Sulivan CV
7th International Symposium on Reproductive Physiology of Fish., May 2003, English, Poster presentation
[International presentation]

Oocyte growth and cytoplasmic maturation of temperate basses: multiple vitellogenins and their receptors.　　　　　　　　　　　　　　　
Hiramatsu N, Hara A, Matsubara T, Hiramatsu K, Sullivan CV
7th International Symposium on Reproductive Physiology of Fish., May 2003, English, Oral presentation
[International presentation]

Vitellogenin in male fish as a bio-marker for estrogenic contamination of the aquatic environment.　　　　　　　　　　　　　　　
Hara A, Hiramatsu N, Soyano K, Matsubara T
Inauguration conference and first academic symposium, CLARINET, Nov. 2001, English, Oral presentation
[International presentation]

Cloning of cDNA for choriogenin H and choriogenin L of masu salmon　　　　　　　　　　　　　　　
Fujita T, FUkada H, Shimizu M, Hiramtasu N, Hara A
70th Anniversary of JSFS, International Commemorative Symposium, Oct. 2001, English, Poster presentation
[Domestic Conference]

マコガレイ血清の雌特異蛋白の動態 ビテロジェニンおよびコリオジェニン
小寺智志, 平松尚志, 藤田敏明, 深田陽久, 藤井一則, 原彰彦
日本水産学会大会講演要旨集, 01 Apr. 2001, Japanese

Estimatio of baseline vitellogenin levels in salmonid male serum　　　　　　　　　　　　　　　
Haga A, Fukada H, Fujita T, Hiramatsu N, Hara A
環境ホルモン学会第3回研究発表会, Dec. 2000, English, Poster presentation
[Domestic Conference]

Vitellogenin-derived yolk proteins of white perch: purification, characterization, and vitellogenin-receptor binding.　　　　　　　　　　　　　　　
Hiramatsu N, Hiramatsu K, Fukada H, Weber GM, Hara A, Sullivan CV
4th International Symposium on FIsh Endocrinnology, Jul. 2000, English, Poster presentation
[International presentation]

Development of chemiluminescent immunoassay for salmonid vitellogenin: serum vitellogenin and estradiol 17beta levels in male cutthroat trout　　　　　　　　　　　　　　　
Fukada H, Haga A, Fujita T, Hiramatsu N, Sullivan CV, Hara A
4th International Symposium on FIsh Endocrinnology, Jul. 2000, English, Poster presentation
[International presentation]

Estrogen induction of choriogenin H and choriogenin L in masu salmon Oncorhynchus masau.　　　　　　　　　　　　　　　
Fujita T, Fukada H, Hiramatsu N, Shimizu M, Haga A, Hara A
4th International Symposium on FIsh Endocrinnology, Jul. 2000, English, Poster presentation
[International presentation]

化学発光イムノアッセイを用いたカットスロートトラウト血中Vg量の測定
芳賀歩, 深田陽久, 藤田敏明, 平松尚志, 木村志津雄, 原彰彦
日本水産学会大会講演要旨集, 01 Apr. 2000, Japanese

マコガレイ血中ビテロジェニンの周年変化　　　　　　　　　　　　　　　
小寺智志, 平松尚志, 藤田敏明, 原彰彦
平成11年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Dec. 1999, Japanese, Poster presentation
[Domestic Conference]

Changes of serum choriogenin and vitellogenin levels in masu salmon (Oncorhynchus masou) during sexual maturation and after estrogen treatment.　　　　　　　　　　　　　　　
Fujita T, Hiramatsu N, Shimizu M, Sullivan CV, Hara A
6th International Symposium on the Reproductive Physiology of Fish., Jul. 1999, English, Poster presentation
[International presentation]

Changes of serum growth hormone and vitellogenin levels in chtthroat trout　　　　　　　　　　　　　　　
Fukada H, Hiramatsu N, Shimizu M, Sullivan CV, Hara A
6th International Symposium on the Reproductive Physiology of Fish., Jul. 1999, English, Poster presentation
[International presentation]

サクラマス卵膜蛋白前駆物質(コリオジェニン)の血中量の変化
藤田敏明, 清水宗敬, 平松尚志, 木村志津雄, 原彰彦
日本水産学会大会講演要旨集, 01 Apr. 1999, Japanese

コイのビテロジェニン酵素免疫測定法(ELISA)の確立
平野香織, 深田陽久, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, 01 Apr. 1999, Japanese

カットスロートの卵黄形成初期におけるビテロジェニンとコリオジェニンについて　　　　　　　　　　　　　　　
芳賀歩, 藤田敏明, 平松尚志, 木村志津雄, 原彰彦
平成10年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Nov. 1998, Japanese, Poster presentation
[Domestic Conference]

ビテロジェニンのcross reaction　　　　　　　　　　　　　　　
高橋みのり, 平松尚志, 原彰彦
平成10年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Nov. 1998, Japanese, Poster presentation
[Domestic Conference]

Fatty acid composition of bony fishe egg and its change in early development.
尾崎雄一, 古賀英裕, 犬飼信子, 宮下和夫, 萱場隆昭, 平松尚志, 原彰彦, 足立伸次, 山内こう平
日本水産学会大会講演要旨集, Apr. 1998, Japanese

Limited degradation enzyme for vitellogenin and vitellus protein in Salmonidae fish.
平松尚志, 市川典正, 木村志津雄, 原彰彦
日本水産学会大会講演要旨集, Apr. 1998, Japanese

サケ科魚類の胚発生に伴う卵黄蛋白および酵素の性状解析　　　　　　　　　　　　　　　
市川典正, 平松尚志, 原彰彦
平成9年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Dec. 1997, Japanese, Poster presentation
[Domestic Conference]

Limited proteolysis of vitellogenin and its derived yolk proteins in salmonids　　　　　　　　　　　　　　　
Hiramatsu N, Ichikawa N, Hosokawa K, Hara A
XIII International Congress of Comparative Endocrinology, Nov. 1997, English, Poster presentation
[International presentation]

Cathepsin-D like proteinase involved in yolk formation in salmonid ovary　　　　　　　　　　　　　　　
Hiramatsu N, Hara A
American Society of Ichthyologists and Herpetologists, 77th Annual meeting, 26 Jun. 1997, English, Poster presentation
[International presentation]

.BETA. component in serum and egg yolk in Salmonidae fishes.
北村真紀子, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, Apr. 1997, Japanese

Change of egg yolk protein accompanied with embryonic development of Salmonidae fishes.
市川典正, 北村真紀子, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, Apr. 1997, Japanese

Purification of Vg degrading enzyme of Salmonidae fishes.
平松尚志, 木村志津雄, 原彰彦
日本水産学会大会講演要旨集, Apr. 1997, Japanese

サクラマス卵の胚発生に伴う卵黄蛋白の変化　　　　　　　　　　　　　　　
市川典正, 北村真紀子, 平松尚志, 木村志津男, 原彰彦
平成8年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Dec. 1996, Japanese, Poster presentation
[Domestic Conference]

サケ科魚類ビテロゲニンの卵黄蛋白質成分への特異的分解　　　　　　　　　　　　　　　
平松尚志, 原彰彦
平成8年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Dec. 1996, Japanese, Poster presentation
[Domestic Conference]

Changes in egg yolk protein accompanied by the embryonic development of Oncorhyncus masou eggs.
市川典正, 北村真紀子, 平松尚志, 木村志津雄, 原彰彦
日本水産学会北海道支部大会講演要旨集, Dec. 1996, Japanese

Peculiarities of serum vitellogenin profiles in Sakhalin taimen　　　　　　　　　　　　　　　
Hiramatsu N, Kitamura M, Hara A
3rd International Symposium on Fish Endocrinology, May 1996, English, Poster presentation
[International presentation]

A sensitive non-competitive avidin-biotin ELISA for chum salmon growth hormone　　　　　　　　　　　　　　　
Fukada H, Hiramatsu N, Hara A
3rd International Symposium on Fish Endocrinology, May 1996, English, Poster presentation
[International presentation]

.BETA.' - component-like protein appearing in serum of Salmonidae fishes.
北村真紀子, 平松尚志, 原彰彦
日本水産学会大会講演要旨集, Mar. 1996, Japanese

Search of enzyme-like protein related to molecular disintegration of vitellogenin of Salmonidae fishes.
平松尚志, 北村真紀子, 木村志津雄, 原彰彦
日本水産学会大会講演要旨集, Mar. 1996, Japanese

Change of serum GH quantity with maturation process of cherry salmon.
深田陽久, 平松尚志, 島崎健二, 原彰彦
日本水産学会大会講演要旨集, Mar. 1996, Japanese

イトウ卵母細胞中の卵膜蛋白成分の検索　　　　　　　　　　　　　　　
藤田敏明, 清水宗敬, 平松尚志, 原彰彦
平成7年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Oct. 1995, Japanese, Poster presentation
[Domestic Conference]

イトウにおけるIgMの精製・定量および寄生性コペポーダsalmincola stellatusに対するspecific IgMの産生　　　　　　　　　　　　　　　
平松尚志, 清水宗敬, 深田陽久, 北村真紀子, 木村志津男, 原彰彦
平成7年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Oct. 1995, Japanese, Poster presentation
[Domestic Conference]

エストラジオール17βによるイトウの卵膜関連蛋白及びビテロジェニンの誘導　　　　　　　　　　　　　　　
清水宗敬, 平松尚志, 原彰彦
平成7年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Oct. 1995, Japanese, Poster presentation
[Domestic Conference]

サクラマスの成熟過程に伴う成長ホルモン及びビテロジェニンの変化　　　　　　　　　　　　　　　
深田陽久, 清水宗敬, 平松尚志, 北村真紀子, 原彰彦
平成7年度道立水産孵化場と北大水産学部との合同セミナー 魚の研究, Oct. 1995, Japanese, Poster presentation
[Domestic Conference]

Three egg yolk proteins are derived from salmonid vitellogenin.　　　　　　　　　　　　　　　
HIRAMATSU Naoshi, HARA Akihiko
The 5th International Symposium on the Reproductive Physiology of Fish, 03 Jul. 1995, English, Poster presentation
[International presentation]

イトウ血清中の卵膜関連蛋白とその誘導
清水宗敬, 平松尚志, 島崎健二, 原彰彦
日本水産学会大会講演要旨集, Apr. 1995, Japanese

サケ成長ホルモンのサンドウイッチ法による酵素免疫測定法
深田陽久, 平松尚志, 玄浩一郎, 原彰彦
日本水産学会大会講演要旨集, Apr. 1995, Japanese

イトウのビテロゲニンは3つの卵黄蛋白へ分子解裂する
平松尚志, 稲場琴美, 木村志津雄, 原彰彦
日本水産学会大会講演要旨集, Apr. 1995, Japanese

回遊魚の増養殖　　　　　　　　　　　　　　　
原彰彦, 木村志津男, 平松尚志, 深田陽久, 清水宗敬
第1回水産学部プロジェクト研究シンポジウム「回遊のメカニズム」, Apr. 1994, Japanese, Public discourse
[Domestic Conference]

Aquaculture production of salmon　　　　　　　　　　　　　　　
Hokkaido University
Oct. 2024 - Present

Fish Aquaculture: ZO586（分担）　　　　　　　　　　　　　　　
North Carolina State University

Fish Physiology: ZO515 (分担）　　　　　　　　　　　　　　　
North Carolina State University

増殖生物学特別実験　　　　　　　　　　　　　　　
北海道大学

増殖生物学演習　　　　　　　　　　　　　　　
北海道大学

増殖生命科学演習　　　　　　　　　　　　　　　
北海道大学

基礎乗船実習　　　　　　　　　　　　　　　
北海道大学

水圏生化学実験　　　　　　　　　　　　　　　
北海道大学

Introduction to Fisheries Sciences　　　　　　　　　　　　　　　
北海道大学

潜水調査実習　　　　　　　　　　　　　　　
北海道大学

比較生理学　　　　　　　　　　　　　　　
北海道大学

増殖生物学特論ＩＩ　　　　　　　　　　　　　　　
北海道大学

増殖生物学特論Ｉ　　　　　　　　　　　　　　　
北海道大学

水族生化学　　　　　　　　　　　　　　　
北海道大学

胎生魚クロソイ雄尿中のフェロモン様物質の同定ならびにその産生および作用機構の解明
科学研究費助成事業
Apr. 2025 - Mar. 2028
東藤 孝, 平松 尚志, 工藤 秀明
日本学術振興会, 基盤研究(C), 北海道大学, 25K09262

養殖サーモンのブランド化と環境負荷低減に貢献する餌料開発および生産物の 健康機能性成分の定量分析　　　　　　　　　　　　　　　
地方大学・地域産業創生交付金事業キングサーモン完全養殖技術研究業務委託
Apr. 2022 - Mar. 2027
平松尚志, 細川雅史, 東藤孝, 高橋勇樹, 藤田雅紀, 深田陽久, 大石岳人
Coinvestigator

Studies toward improvement/acquirement of carotenoid deposition in muscle of white meat fish
Grants-in-Aid for Scientific Research
30 Jun. 2023 - 31 Mar. 2026
平松 尚志, 東藤 孝, 藤田 雅紀
本研究では、魚類カロテノイド分解に関わる遺伝子候補に着目し、その翻訳制御機構を解明すると共に、同候補のゲノム編集が身色形質の改善に有効か否かを試すことを目的とする。目的達成に向け、「スカベンジャー受容体クラスBタイプ1（srb1）を介したアスタキサンチン（Ax）取込み量を増やし、βカロテンオキシゲナーゼ（bco）による分解を抑えることができれば、最終的に筋肉のAx蓄積量が増え赤色呈色性が強くなる」と仮説を立て、これを直接的にゲノム編集により実証する課題1と、未知な点が多いsrb1及びbcoの翻訳制御機構を明らかにする課題2を実施する。
R5年度は、公開ゲノムデータベースを探索し、ニジマスではbco1, bco1l, srb1遺伝子、メダカではbco1とbco1l遺伝子について、各ゲノム上の位置や配列を特定した。この情報を基に、課題1では、各遺伝子のガイドRNA（gRNA）を設計・合成した。これらのgRNAとCas9ヌクレアーゼを用いて、ニジマス及びメダカの受精卵に顕微注入し作出したF0胚について、ヘテロ二本鎖移動度分析（HMA）とシーケンス解析結果、両種ともにbco1lに関しては、変異導入個体が確認できた。一方で、その他の遺伝子については、変異導入個体の作出に至らなかった。課題2に関しては、両種から上記遺伝子転写産物のcDNAクローニングを行い、全てのクローニングを完了した。メダカbco1lについては、組換え蛋白質とその抗体を作製し、抗原組換え蛋白質との免疫陽性反応を得た。この抗体を用いて、消化管試料のウェスタンブロッティングを実施したが、陽性反応は確認できなかった。現在、bco1l以外の遺伝子由来の組換え蛋白質の作製も継続中である。一方、身色の赤色呈色を獲得前の若年魚と同呈色獲得後の出荷魚を用い、消化管におけるmiRNA発現データの取得を行った。
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, 23K18041

魚卵アレルゲン低減化魚類の提案と最適な飼育環境の確立
科学研究費助成事業
01 Apr. 2023 - 31 Mar. 2026
清水 裕, 笹岡 友季穂, 平松 尚志, 佐伯 宏樹, 清水 宗敬
日本学術振興会, 基盤研究(C), 北海道大学, 23K05125

フィールドサイエンスを基盤とした地球環境を再生する新たな持続的食料生産システムの構築と展開　　　　　　　　　　　　　　　
地域中核・特色ある研究大学強化促進事業（J-PEAKS）
Apr. 2024
Coinvestigator

Analysis of vitellogenesis in fish using genome genome editing technology
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2021 - 31 Mar. 2024
東藤 孝, 平松 尚志
本研究は、魚類の卵母細胞における広義の卵黄物質、すなわち中性脂質（油球）と卵黄タンパク質（卵黄球）の蓄積機構について、それらに関わる重要な分子群の機能を最先端のゲノム編集技術を用いることにより解析し、明らかにすることを目的としている。メダカを研究モデルとし、「課題1：油球形成機構の解析」と「課題2：卵黄球形成機構の解析」の2課題を設定して研究を進めている。
課題1：先ず、油球を構成する中性脂質の主要な供給源である超低密度リポタンパク質の代謝酵素（リポタンパクリパーゼ：Lpl）に着目し、メダカ卵濾胞組織におけるlpl遺伝子の発現を解析した。その結果、2種類のlpl遺伝子（lpl1とlpl2）のうち、lpl1のみが発現していることが示されたことから、lpl1の遺伝子欠損（KO）メダカを作出することとした。CRISPR/Cas9システムによる効率的な変異導入を達成するため、lpl１遺伝子に対する複数のガイドRNA（gRNA）候補を設計して検討した結果、フレームシフトを伴う変異導入に有効なgRNAの組み合わせが見出された。
課題2：卵黄タンパク前駆物質（ビテロジェニン：Vtg）の卵内への取り込みに必要な3種のリポタンパク質受容体（Lr7、Lr8、Lrp13）のそれぞれについて、メダカ卵濾胞での発現解析ならびに、KO魚の作出とそれらの表現型解析を進めている。これまでに、Lr8のKOメダカ系統（lr8-KO）の作出に成功し、その表現型解析を行った。その結果、lr8-KO系統メダカの孵化仔魚の生残率が野生型のそれよりも低下することが示された。さらにlr8-KO系統メダカにおいては、4種のVtg（VtgAa1、VtgAa2、VtgAb、VtgC）のうち、VtgAb由来の卵黄タンパク質成分が野生型と比べて相対的に減少しており、Lr8がVtgAbタイプの主要な受容体であることが明らかとなった。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 21K05764

大型トラウトサーモン（ニジマス）の量産化に向けた 高効率配合飼料技術体系の研究開発　　　　　　　　　　　　　　　
養殖業成長産業化提案公募型実証事業
2023 - 2024
水産庁マリノフォーラム21, Coinvestigator

育種・染色体操作を用いたサケ科魚類の魚卵アレルゲン性低減化の試み
科学研究費助成事業 基盤研究(C)
01 Apr. 2020 - 31 Mar. 2023
清水 裕, 笹岡 友季穂, 藤本 貴史, 平松 尚志, 石田 晃彦, 渡慶次 学, 佐伯 宏樹
進捗状況を3項目に分けて述べる。
1.不妊魚の作出：ニジマスの卵と凍結保存したブラウントラウトあるいはサクラマスの精子を受精し、第二極体放出阻止処理により作出された雑種三倍体候補（A：ニジマス×ブラウントラウト、B：ニジマス×サクラマス）の倍数性を調査した。その結果、候補Aでは全25個体で、候補Bでは1個体を除く30個体が三倍体であった。これらの個体はPITタグで標識し、現在も継続して飼育している。（藤本）
2.魚卵アレルゲン検知系の構築：引き続き、ニジマス卵アレルゲンであるβ’-component（BC）のペーパー免疫分析デバイスの構築に取り組んだ。デバイスは濾紙にインクを印刷して加熱する常法により作製し、インクで囲まれた領域を分析反応ゾーンとした。分析は、反応ゾーンに新規作製した抗BC抗体を固定化して試料、酵素標識抗BC抗体、発色試薬を順に加えて行う手順とした。本手法では、従来並みの感度（検出感度：約1 ng/mL）の分析が従来の1/100の時間（約20分）で可能となった。（渡慶次、石田）
加えて、交雑種に対応した検知系に使用する抗体の作成のため、サクラマス排卵からBCを精製し、これを家兎に免疫し抗血清を作製した。（平松）
3.不妊化魚の魚卵アレルゲン性の調査：全23個体の不妊化三倍体ニジマスの内臓組織に含まれるBCを測定したが、全個体の筋肉には魚卵アレルギー発症リスクが認められなかった。しかし、5個体において生殖線から少量のBCが検出されたが、その内3個体は目視にて明確な生殖腺の発達が観られた。目視で生殖線の発達が確認できなかった20個体について、生殖腺の組織切片を作製・観察し、その発達状況を確認した。その結果、３個体で発達中の卵母細胞が散在しているのが確認され、生殖腺の外観だけでアレルギー発症リスクの有無を見分けるのは困難であることが判明した。（清水、佐伯、笹岡、平松）
日本学術振興会, 基盤研究(C), 北海道大学, 20K05903

養殖サケマス身肉への色素蓄積制御に関する生物情報科学的研究　　　　　　　　　　　　　　　
令和4年度学術研究支援事業
Apr. 2022 - Mar. 2023
平松尚志
南北海道学術振興財団, 北海道大学, Principal investigator

雄尿関連蛋白質を利用した北方性メバル類の養殖・育種効率化　　　　　　　　　　　　　　　
令和3年度研究助成
Apr. 2021 - Mar. 2022
平松尚志
寿原記念財団, 北海道大学, Principal investigator

魚卵を標的とした物質輸送システムの開発：バイオリアクターによる輸送体の大量生 産
科学研究費補助金 基盤研究Ｂ
2018 - 2022
平松 尚志
文部科学省, Principal investigator, Competitive research funding

Basic studies toward smart cell industry of salmon species　　　　　　　　　　　　　　　
令和2年度助成事業
Apr. 2020 - Mar. 2021
平松 尚志
Hokusui Foundation, 北海道大学, Principal investigator

幻の魚イトウの海中養殖法確立のための有用遺伝子マーカーの探索　　　　　　　　　　　　　　　
助成金
Apr. 2019 - Mar. 2020
東藤 孝, 者, 平松
南北海道学術振興財団, Competitive research funding

生物情報科学によるメバル属魚類の増養殖関連有用マーカーの探索　　　　　　　　　　　　　　　
助成金
Apr. 2018
平松 尚志
南北海道学術振興財団, Principal investigator, Competitive research funding

フサカサゴ科メバル属魚類の性成熟度判定および繁殖制御技術の開発　　　　　　　　　　　　　　　
研究助成事業
Apr. 2017
川崎琢真
北水協会, Competitive research funding

Studies on mechanisms underlying formation of lipid droplets in oocytes of teleost fishes
Grants-in-Aid for Scientific Research
01 Apr. 2014 - 31 Mar. 2017
Todo Takashi, HARA Akihiko, HIRAMATSU Naoshi
We have previously shown about the mechanisms underlying formation of lipid droplets in teleost oocytes as follows; plasma very low density lipoprotein is the major carrier of neutral lipids to oocyte lipid droplets, and Vldl is metabolized at the outside of oocyte, then free fatty acids derived from the Vldl metabolism are incorporated into oocyte. In this study, we focused on the role of lipoprotein lipase (Lpl), a key enzyme of Vldl metabolism, and analyzed its expression pattern in ovarian follicle using salmonids, medaka, pipefish and zebrafish. As the results, it was suggested that the presence of Lpl expressed in ovarian follicle cells is indispensable for the formation of lipid droplets in oocytes.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 26450280

カレイ類受精卵及びシシャモ仔稚魚に対する種特異抗体の作製　　　　　　　　　　　　　　　
研究助成事業
Apr. 2015 - Mar. 2017
平松 尚志
北水協会, Principal investigator, Competitive research funding

魚類の卵母細胞を標的とする分子輸送体を産生するトランスジェニックメダカの作製　　　　　　　　　　　　　　　
科学研究費補助金 挑戦的萌芽研究
Apr. 2015 - Mar. 2017
平松 尚志
文部科学省, Principal investigator, Competitive research funding

Hepatoprotective effects of novel antioxidant isolated from the Pacific oyster
Grants-in-Aid for Scientific Research
01 Apr. 2013 - 31 Mar. 2016
Fuda Hirotoshi, HIRAMATSU Naoshi, JIN Shigeki
This study was undertaken to characterize the hepatoprotective potency of 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), a novel phenolic antioxidant, isolated from the Pacific oyster, using hepatoma-derived cells (C3A) cultured in the presence of pro-oxidant, as well as well-known antioxidants. We found that only DHMBA significantly protected C3A cells against cell death in a dose-dependent manner, whereas other antioxidants did not. Also our study assessed the effects of DHMBA-rich oyster extracts (DOE) in a novel non-alcoholic steatohepatitis (NASH) model mouse established by combination of a high-fat diet and oxidized low-density lipoprotein. The NASH model mouse exhibited obesity, insulin resistance, hepatic steatosis, inflammation, fibrosis, and apoptosis. These changes were significantly moderated by supplementation of DOE. From these results, DHMBA might serve as a functional food for people at elevated risk for ROS-related diseases, such as NASH.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 25450297

チョウザメの生殖統 御技術開発のための 性分化、卵成長およ び卵成熟の分子機構 解析　　　　　　　　　　　　　　　
科学研究費補助金 基盤研究Ａ
Apr. 2012 - Mar. 2015
足立 伸次
文部科学省, Competitive research funding

マガキで発見された 新規の抗酸化物質に よる非アルコール性 脂肪肝炎の予防効果　　　　　　　　　　　　　　　
科学研究費補助金 基盤研究Ｃ
Apr. 2012 - Mar. 2014
布田博敏
文部科学省, Competitive research funding

エピトープ情報を活 用した魚卵アレルゲ ン検知系の開発　　　　　　　　　　　　　　　
科学研究費補助金 基盤研究Ｃ
Apr. 2012 - Mar. 2014
佐伯 宏樹
文部科学省, Competitive research funding

Basic studies on development of a novel delivery system of materials into fish oocytes
Grants-in-Aid for Scientific Research
Apr. 2011 - Mar. 2013
HIRAMATSU Naoshi, GEN Koichiro
A yolk precursor vitellogenin (Vg) is selectively incorporated from the maternal blood into oocyte following its binding to ovarian Vg receptor. This study demonstrated that Vg obtained from heterologous species, as well as Vg coupled with voluntary lipids, can be delivered to fish eggs and offsprings by injecting them into broodstocks. This indicated a potential use of fish Vg as a transporter of bioactive materials; the system can be universal to any fish species. This study also confirmed that such system was found to be applicable for the delivery of bioactive lipids and/or hydrophobic materials to eggs and offsprings across generations.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 23580243

魚類の卵黄球および油球形成機構の解明　　　　　　　　　　　　　　　
科学研究費補助金 基盤研究Ｂ
Apr. 2010 - Mar. 2013
原 彰彦
文部科学省, Competitive research funding

Studies on molecluar mechanism of lipid droplet formation in telesot oocytes
Grants-in-Aid for Scientific Research
2011 - 2013
TODO TAKASHI, HARA Akihiko, HIRAMATSU Naoshi
In teleost fishes, high amount of lipids and proteins are accumulated in oocytes during their growth phase; they are later utilized as energy and nutrient resources by developing embryos and larvae. However, little is known about the origin of such lipids and the mechanisms underlying their accumulation into oocytes. In the present study, we aimed to clarify the mechanisms at the molecular levels. As the results, we confirmed that, for the first time, plasma very low density lipoprotein is the major carrier of neutral lipid to oocyte lipid droplets, and updated the model for the oocyte lipidation.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 23580242

卵巣特異リポ蛋白質受容体を標的とした物質輸送:光る魚の大量生産
産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 探索タイプ
2011 - 2011
平松 尚志
本研究は蛍光性物質を親魚から卵・稚仔魚へ輸送する技術の開発を目指し、1)卵母細胞に特異的に発現する2種類の受容体を標的とする抗体の作製、および2)蛍光標識抗体の生体投与試験を行った。実施においては計画した全ての実験を完了した。結果においては、これら抗体によるゼブラフィッシュの卵母細胞や受精卵・孵化仔魚への蛍光物質の輸送は殆ど検出できなかった。一方、本研究と並行した実験により、受容体に対する抗体ではなくリガンドを輸送体に使用した場合、卵母細胞および受精卵・孵化仔魚への蛍光物質の輸送が確認された。以上の結果より、輸送体にはリガンドを使用するべきであり、またその場合、蛍光物質の輸送が技術的に可能であることが示された。
北海道大学

Studies on vitellogenin and its receptor in salmonid using interactome techniques
Grants-in-Aid for Scientific Research
2009 - 2010
HIRAMATSU Naoshi
When fish grows ovaries, a yolk precursor vitellogenin (Vg) is incorporated from the blood into oocyte following its binding to ovarian Vg receptor (VgR). This study first identified a portion of VgR-binding region on salmon Vg (sVg) using cutting-edged interactome techniques. Further, a novel C-type Vg (VgC) was cloned and its recombinant protein was synthesized with a limited amount; this provided a basis for discovering the unrevealed VgC receptor.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 21780172

Studies on mechanism of lipid uptake into teleost oocytes
Grants-in-Aid for Scientific Research
2008 - 2010
TODO Takashi, HARA Akihiko, HIRAMATSU Naoshi
In teleost fishes, high amounts of lipids and proteins are accumulated in oocytes during their growth phase ; they are later utilized as energy and nutrient resources by developing embryos and larvae. However, little is known about the origin of such lipids and the mechanisms underlying their accumulation into oocytes. In the present study, we aimed to clarify the mechanisms at the molecular levels. As the results, we identified various factors involved in the lipid uptake into fish oocytes, and proposed a new model for the oocyte lipidation.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20580192

Hybrid striped bass farming: Applications of genomics and functional proteomics to solve problems in growth and reproduction　　　　　　　　　　　　　　　
国際研究協力
2006 - 2010
Competitive research funding

Studies of fish vitellogenesis based on the multiple vitellogenin model
Grants-in-Aid for Scientific Research(基盤研究(B))
2007 - 2009
Akihiko HARA, 松原孝博, 征矢野清, 東藤孝, 平松尚志, Takahiro MATSUBARA, Kiyoshi SOYANO, Takashi TODO, Naoshi HIRAMATSU
The objective of this study is to elucidate mechanisms involved in the yolk formation in fish oocytes. Results included development of novel technologies for separation and quantification of multiple subtypes of a yolk precursor (vitellogenin : Vg) ; such techniques were initially developed for the grey mullet, and thereafter applied for several other teleosts. Findings endow the better understandings on fish oogenesis, as well as improvement of fish egg quality in aquaculture and development of technologies for the surveys on the impacts of environmental xenoestrogenic chemicals.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 19380106

Studies on the specific binding of vitellogenin and very-low-density lipoprotein to ovarian lipoprotein receptor(s) in teleosts
Grants-in-Aid for Scientific Research(若手研究(B))
2007 - 2008
Naoshi HIRAMATSU
本研究は、魚卵中の卵黄についての研究であり、特に卵黄の素となる血液中の前駆体(ビテロジェニンと超低密度リポ蛋白質)が、どの様に卵へ取り込まれるかを調べた。スズキ類とボラ類の魚を使いこれらの蛋白質や遺伝子を解析した結果、まずビテロジェニンには3種類のサブタイプがあることが明らかとなった。また各種ビテロジェニンと超低密度リポ蛋白質は、それぞれ異なる受容体と結合してから卵の中に取り込まれる可能性が初めて示された。
Ministry of Education, Culture, Sports, Science and Technology, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 19780143

サケ科魚類の多型ビテロジェニンおよびコリオジェニンに対する免疫測定系の確立　　　　　　　　　　　　　　　
長期自主研究
2007 - 2008
第8期日本化学工業協会, Competitive research funding

魚類の新たな生体指標蛋白を利用した環境エストロジェン評価系の構築　　　　　　　　　　　　　　　
科学技術振興調整費
2007 - 2007
平松 尚志
Principal investigator, Competitive research funding

魚類ビテロゲニンの卵母細胞への蓄積ならびに稚仔魚への吸収機構の解明　　　　　　　　　　　　　　　
特別研究員研究費（PD）
Apr. 1998
平松 尚志
日本学術振興会, Principal investigator, Competitive research funding

サケ科魚類におけるビテロゲニンの卵黄蛋白への分子解裂機構の解明　　　　　　　　　　　　　　　
DC2
1996 - 1997
平松 尚志
日本学術振興会, Principal investigator, Competitive research funding

Deposition and utilization of vitellogenin and yolk proteins in teleost fish.　　　　　　　　　　　　　　　
Competitive research funding

函館朝市ミニ水族館　　　　　　　　　　　　　　　
15 Jul. 2019 - 30 Dec. 2019
Advisor, Organizing member
Seminar
日本財団
はこだて海の教室～海と日本PROJECT~

魚の卵を科学しよう！　　　　　　　　　　　　　　　
05 Aug. 2019
Lecturer, Advisor, Planner, Organizing member
Seminar
北海道大学
令和元年度オープンキャンパス【高校生限定プログラム（実験）】

２つの海に面する八雲：日本海と太平洋の海の生物・環境を比べてみよう –海と日本PROJECTー　　　　　　　　　　　　　　　
15 Jul. 2019
Lecturer, Advisor, Planner, Organizing member, Report writing
Seminar
日本財団
海の宝アカデミックコンテスト2019・ブルーオーシャン活動に向けて（海と日本2019）

魚の卵を科学する　　　　　　　　　　　　　　　
27 Jun. 2019
Lecturer
Lecture
北海道高等学校理科研究会
北海道高等学校理科研究会支部総会一般講演

海の宝アカデミックコンテスト2018　　　　　　　　　　　　　　　
10 Nov. 2018
Planner, Organizing member
Seminar
日本財団
H30年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

北の海の七福神（キラリス）ミニ水族館　　　　　　　　　　　　　　　
Jul. 2018 - Sep. 2018
Advisor, Organizing member
Others
日本財団
H30年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

北の海の七福神（函館空港）ミニ水族館　　　　　　　　　　　　　　　
01 Aug. 2018 - 31 Aug. 2018
Advisor, Organizing member
Others
日本財団
H30年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

海って結構面白い 海の写真展とワークショップ　　　　　　　　　　　　　　　
06 Aug. 2018 - 12 Aug. 2018
Organizing member
Seminar
日本財団
H30年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

マリン・ラーニング 魚拓づくり　　　　　　　　　　　　　　　
21 Jul. 2018
Presenter, Planner, Organizing member
Seminar
日本財団
H30年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

海の宝アカデミックコンテスト2017　　　　　　　　　　　　　　　
Nov. 2017
Planner, Organizing member, Contribution
Seminar
日本財団
H29年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

魚の卵を科学しよう！　　　　　　　　　　　　　　　
07 Aug. 2017
Appearance, Presenter, Lecturer, Planner, Organizing member, Demonstrator
Seminar
北海道大学
平成29年度オープンキャンパス【高校生限定プログラム（実験）】

実験に参加！！函館海洋センターバックヤード研修　　　　　　　　　　　　　　　
29 Jul. 2017 - 30 Jul. 2017
Presenter, Planner, Organizing member, Demonstrator
Seminar
日本財団
H29年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

マリン・ラーニング 魚拓づくり　　　　　　　　　　　　　　　
16 Jul. 2017
Presenter, Planner, Organizing member
Seminar
日本財団
H29年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

海の宝アカデミックコンテスト2016　　　　　　　　　　　　　　　
Nov. 2016
Planner, Organizing member
Seminar
日本財団
H28年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング：函館マリンフェスティバル

パドルボードで海の宝ものを探そう　　　　　　　　　　　　　　　
06 Aug. 2016 - 07 Aug. 2016
Planner, Organizing member
Seminar
日本財団
H28年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング

魚の卵を科学する　　　　　　　　　　　　　　　
30 Jul. 2016
Lecturer
Lecture
北海道大学
平成28年度（第30回）北海道大学水産学部公開講座「弁天町発！！水産・海洋研究の最前線」

北の魚の赤ちゃんと海藻の世界　　　　　　　　　　　　　　　
23 Jul. 2016 - 24 Jul. 2016
Appearance, Presenter, Lecturer, Planner, Organizing member, Demonstrator
Seminar
日本財団
H28年度 日本財団「海と日本プロジェクト」：海の宝を巡る学びと体験マリンラーニング：函館マリンフェスティバル

魚の卵を科学しよう！　　　　　　　　　　　　　　　
03 Aug. 2015
Appearance, Presenter, Lecturer, Advisor, Planner, Organizing member, Demonstrator
Seminar
北海道大学
平成27年度オープンキャンパス【高校生限定プログラム（実験）】

魚の卵を科学しよう！　　　　　　　　　　　　　　　
2013
Appearance, Presenter, Lecturer, Advisor, Planner, Organizing member, Demonstrator
Seminar
北海道大学
平成25年度オープンキャンパス【高校生限定プログラム（実験）】

魚の増える仕組み　　　　　　　　　　　　　　　
23 May 2012
Presenter, Lecturer, Planner, Organizing member, Demonstrator
Seminar
北海道大学
新水産・海洋都市はこだてを支える人材育成、H24年度海のものづくりプログラム

科学論文雑誌審査員　　　　　　　　　　　　　　　
Others
Biochimica Biophysica Acta, Biology of Reproduction, Comparative Biochemistry and Physiology, Fish Physiology and Biochemistry, General Comparative Endocrinology, Journal of Experimental Biology, Journal of Histochemistry and C・・・

メバル類の成熟度をタンパク質で判別 北大水産院の山口さんら 人工授精効率化に期待　　　　　　　　　　　　　　　
28 Feb. 2024
北海道新聞
北海道新聞
[Internet]

胎生メバル類の雄成熟度マーカーの開発に初めて成功～メバル類増養殖における種苗生産の効率化への貢献に期待～　　　　　　　　　　　　　　　
14 Feb. 2024
Myself
北海道大学
プレスリリース
[Others]

釣りびと万歳トークショー～釣りびとたちと考える道南の海～　　　　　　　　　　　　　　　
09 Oct. 2022
NHK
NHK函館放送局 開局90周年 見ささるFes.
[Media report]

「メバル」　　　　　　　　　　　　　　　
30 Sep. 2022
NHK Eテレ
ギョギョッとサカナ★スター

パネルディスカッション　　　　　　　　　　　　　　　
17 Sep. 2017
Panel chair etc, Panel chair etc, Planning etc, Planning etc
北海道大学

2023
ソイ・メバル (Rockfish)
水産学部HP上にFish of the Monthコンテンツとして記事掲載

2014 - 2014
日本水産学会春季大会
会場担当

2010 - 2010
日本水産学会北海道支部大会
評議委員会・総会担当

2009 - 2009
日本水産学会北海道支部大会
本部・会場担当

2007 - 2007
日本水産学会秋季大会
会場担当

2004 - 2004
ハワイ大学サマープログラム
米国ハワイ大学における夏期プログラム（Edwin W. Pauley Summer Program in Marine Biology)に出席し、講義および研究指導（Real-time PCRによるティラピアビテロジェニン遺伝子発現定量法の確立）を行った。