Oct. 2015, 日本歯科医学会, 第31回「歯科医学を中心とした総合的な研究を推進する集い」優秀発表賞　　　　　　　　　　　　　　　
「歯表面での3D微細構造構築によるバイオミメティック次世代歯科治療へ向けて」
赤坂 司

Oct. 2013, 日本歯科理工学会, 第６１回日本歯科理工学会 発表優秀賞　　　　　　　　　　　　　　　
「マイクロ・ナノ構造を持つパターン化バイオマテリアルの調製」
赤坂 司

Dec. 2008, "Materials Science and Engineering C" Most Cited Author Award 2005-2008.　　　　　　　　　　　　　　　
Title: "Apatite formation on carbon nanotubes" T.Akasaka, F.Watari, Y.Sato, K.TohjiMaterials Science and Engineering C, 26(4), 675-678 (2006).

Oct. 2006, 日本歯科理工学会, 第48回 日本歯科理工学会 研究奨励賞　　　　　　　　　　　　　　　
「人工複合糖質高分子によるStreptococcus mutansの増殖阻害」
Japan

2006, Molecular Biosystems Cover Gallery Winner 2005　　　　　　　　　　　　　　　

Oct. 2004, 傾斜機能材料研究会, 傾斜機能材料シンポジウム FGM奨励賞　　　　　　　　　　　　　　　
「糖鎖によるカーボンナノチューブの表面修飾」
赤坂 司

Pharmacokinetics and the effectiveness of pyrogen-free bioabsorbable wet adhesives.
Risa Oshima, Kumiko Yoshihara, Ko Nakanishi, Tsukasa Akasaka, Shinji Shimoji, Teppei Nakamura, Takumi Okihara, Mariko Nakamura, Akihiro Matsukawa, Ikkei Tamada, Bart Van Meerbeek, Tsutomu Sugaya, Yasuhiro Yoshida
Scientific reports, 15, 1, 20056, 20056, 12 Jun. 2025, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Bioabsorbable materials are essential for advanced therapies, including surgical sealing, cell therapy, and drug delivery. Natural bioabsorbable materials, including collagen and hyaluronic acid, have better biocompatibility than synthetic bioabsorbable polymers; however, they are mainly derived from animals, presenting infection risks. Non-animal origin polymers have a lower molecular weight than those of animal origins. Their viscosity increases with increase in molecular weight, making endotoxin removal difficult. Here, using the phosphoryl chloride disposal method, we present a strategy for synthesizing pyrogen-free bioabsorbable adhesives with controlled molecular weight. Phosphopullulan, a polysaccharide derivative, had less than detectable endotoxin levels and controllable average molecular weight of approximately 300,000 to over 1,400,000. Furthermore, it is important to ensure the safety as well as efficacy of bio-implantable materials. We have evaluated the biosafety of polysaccharide derivatives we are developing, and have examined their cell phagocytosis and pharmacokinetics in vitro and in vivo, and have confirmed that they are safe. We have also evaluated their adhesion to wet tissue adhesions and confirmed that they leak less than existing materials.

New future dental material: Antimicrobial material "cetylpyridinium chloride-montmorillonite" and implantable material "phosphorylated pullulan".
Ko Nakanishi, Tsukasa Akasaka, Yasuhiko Abe, Hiroshi Hayashi, Kumiko Yoshihara, Teppei Nakamura, Mariko Nakamura, Bart VAN Meerbeek, Yasuhiro Yoshida
Dental materials journal, 11 Jan. 2025, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, In dental practice, there are two major diseases: dental caries and periodontal disease. Although dental treatment techniques have advanced along with advances in dental materials, some diseases such as root surface caries and horizontal bone resorption have not yet achieved satisfactory treatment results. Since these diseases are infections caused by oral bacteria, we believe that materials with long-lasting antimicrobial properties would help control these diseases. In addition, materials that can adhere to wet hard tissues would contribute to treatment. In this review, new materials developed based on this idea, the antimicrobial material "cetylpyridinium chloride-montmorillonite" and the hard tissue adhesive implantable material "phosphorylated pullulan" was introduced.

Synthesis and evaluation of fluorescent resins with europium-β-diketonate complex for orthodontic use
Shuichi YAMAGATA, Takaaki YAMAMOTO, Yoshiaki SATO, Tsukasa AKASAKA, Yasuhiro YOSHIDA
Dental Materials Journal, 43, 4, 531, 538, Japanese Society for Dental Materials and Devices, 25 Jul. 2024, [Peer-reviewed], [International Magazine]
Scientific journal

From Tooth Adhesion to Bioadhesion: Development of Bioabsorbable Putty-like Artificial Bone with Adhesive to Bone Based on the New Material "Phosphorylated Pullulan".
Ko Nakanishi, Tsukasa Akasaka, Hiroshi Hayashi, Kumiko Yoshihara, Teppei Nakamura, Mariko Nakamura, Bart Van Meerbeek, Yasuhiro Yoshida
Materials (Basel, Switzerland), 17, 15, 25 Jul. 2024, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Bioabsorbable materials have a wide range of applications, such as scaffolds for regenerative medicine and cell transplantation therapy and carriers for drug delivery systems. Therefore, although many researchers are conducting their research and development, few of them have been used in clinical practice. In addition, existing bioabsorbable materials cannot bind to the body's tissues. If bioabsorbable materials with an adhesive ability to biological tissues can be made, they can ensure the mixture remains fixed to the affected area when mixed with artificial bone or other materials. In addition, if the filling material in the bone defect is soft and uncured, resorption is rapid, which is advantageous for bone regeneration. In this paper, the development and process of a new bioabsorbable material "Phosphorylated pullulan" and its capability as a bone replacement material were demonstrated. Phosphorylated pullulan, which was developed based on the tooth adhesion theory, is the only bioabsorbable material able to adhere to bone and teeth. The phosphorylated pullulan and β-TCP mixture is a non-hardening putty. It is useful as a new resorbable bone replacement material with an adhesive ability for bone defects around implants.

Epithelial-mesenchymal transition in oral cancer cells induced by prolonged and persistent Fusobacterium nucleatum stimulation
Shintaro Nakano, Machiko Kasai, Keisuke Nakamura, Tsukasa Akasaka, Yasuhiro Yoshida, Shiho Suzuki, Yoichi Ohiro, Akira Hasebe
Journal of Oral Biosciences, Elsevier BV, May 2024, [Peer-reviewed], [International Magazine]
Scientific journal

Investigation of a new implant surface modification using phosphorylated pullulan.
Kanako Nagamoto, Ko Nakanishi, Tsukasa Akasaka, Shigeaki Abe, Kumiko Yoshihara, Mariko Nakamura, Hiroshi Hayashi, Shinji Takemoto, Masato Tamura, Yoshimasa Kitagawa, Bart Van Meerbeek, Yasuhiro Yoshida
Frontiers in bioengineering and biotechnology, 12, 1378039, 1378039, 2024, [Peer-reviewed], [International Magazine]
English, Scientific journal, Various implant surface treatment methods have been developed to achieve good osseointegration in implant treatment. However, some cases remain impossible to treat with implants because osseointegration is not obtained after implantation, and the implants fail. Thus, this study focused on phosphorylated pullulan because of its adhesiveness to titanium (Ti) and bone, high biocompatibility, and early replacement with bone. In this study, the response of bone-related cells to phosphorylated pullulan was evaluated to develop a new surface treatment method. Saos-2 (human osteosarcoma-derived osteoblast-like cells), MC3T3-E1 (mouse calvaria-derived osteoblast-like cells), and RAW264.7 (mouse macrophage-like cells) were used. In evaluating cellular responses, phosphorylated pullulan was added to the culture medium, and cell proliferation and calcification induction tests were performed. The proliferation and calcification of cells on the surface of Ti disks coated with phosphorylated pullulan were also evaluated. In addition, bone morphogenetic protein-2 (BMP-2), an osteogenic factor, was used to evaluate the role of phosphorylated pullulan as a drug carrier in inducing calcification on Ti disks. Phosphorylated pullulan tended to promote the proliferation of osteoblast-like cells and the formation of calcification on Ti disks coated with phosphorylated pullulan. Ti disks coated with phosphorylated pullulan loaded with BMP-2 enhanced calcification. Phosphorylated pullulan inhibited osteoclast-like cell formation. These results are due to the properties of phosphorylated pullulan, such as adhesiveness to titanium and drug-loading function. Therefore, phosphorylated pullulan effectively promotes bone regeneration when coated on titanium implants and is useful for developing a new surface treatment method.

微細構造に応答した破骨細胞が作り出す多様なアクチン構造物　　　　　　　　　　　　　　　
赤坂 司, 中西 康, 吉田 靖弘
日本歯科理工学会誌, 42, Special Issue WINTER, 5, 5, (一社)日本歯科理工学会, Dec. 2023, [Peer-reviewed], [Lead author], [Domestic magazines]
Japanese

破骨細胞様細胞による凹凸基材に応答したアクチン構造物の形成　　　　　　　　　　　　　　　
赤坂 司, 宮治 裕史, 横山 敦郎, 吉田 靖弘
日本バイオマテリアル学会大会予稿集, 45回, 422, 422, 日本バイオマテリアル学会, Oct. 2023, [Peer-reviewed], [Lead author], [Domestic magazines]
Japanese

In vivo transmission electron microscopy of the alternating structure of the protrusions between adjacent macrophage-like cells on micropatterns.
Natsumi Ushijima, Tsukasa Akasaka, Shinichiro Numamoto, Tsubura Kudo, Atsuro Yokoyama
Journal of oral biosciences, 29 Jun. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVES: We investigated the behavior of macrophages in the defined microtopography of materials. METHODS: Patterned cyclo-olefin polymer films were implanted into the femurs of seven-week-old rats. After 1 and 4 weeks, the rats were fixed with glutaraldehyde and OsO4, and their bones were observed with transmission electron microscopy (TEM). RESULTS: TEM and segmentation revealed an alternating structure in which multiple protrusions of adjacent macrophage-like cells overlapped. They were approximately 2 μm long and almost uniform in width, and were induced by the limited topography. CONCLUSION: New structures appeared between the macrophage-like cells as a result of microtopography.

Can a low dosage of recombinant human bone morphogenetic protein-2 loaded on collagen sponge induce ectopic bone?
Mohammed Katib Alruwaili, Tsutomu Sugaya, Yasuhito Morimoto, Ko Nakanishi, Tsukasa Akasaka, Yasuhiro Yoshida
Dental materials journal, 42, 3, 433, 440, 07 Apr. 2023, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Recombinant human bone morphogenetic protein-2 (rhBMP-2) is one of the growth factors that may induce the formation of new bone. The aim was to determine the efficacy of low doses of rhBMP-2 for bone regeneration using a collagen sponge as a carrier. Three doses of rhBMP-2 (1.167, 0.117, and 0.039 mg/mL) were combined with an absorbable collagen sponge (ACS) as a delivery vehicle. The rhBMP-2/ACS implants were placed in the subcutaneous tissues of rat backs. X-ray microcomputed tomography (micro-CT) and histological analysis were used to evaluate bone formation. The samples treated with 1.167 mg/mL of rhBMP-2 showed greater bone formation than the samples treated with 0.117 mg/mL of rhBMP-2 four weeks after surgery. However, there was no evidence of bone formation in the samples that were treated with 0.039 mg/mL of rhBMP-2. It was found that rhBMP-2 was osteogenic even at one-tenth of its manufacturer's recommended concentration (1.167 mg/mL), indicating its potential for clinical use at lower concentrations.

ヒトI型コラーゲン様リコンビナントペプチド/β-三リン酸カルシウム骨補填材による歯周組織再生効果
吉野 友都, 宮治 裕史, 西田 絵利香, 金本 佑生実, 浜本 朝子, 加藤 昭人, 菅谷 勉, 赤坂 司
日本歯周病学会会誌, 65, 春季特別, 133, 133, (NPO)日本歯周病学会, Apr. 2023, [Peer-reviewed], [Domestic magazines]
Japanese

破骨細胞分化へのパターン材質と前駆細胞種の影響　　　　　　　　　　　　　　　
赤坂 司, 中西 康, 吉田 靖弘
日本歯科理工学会誌, 42, Special Issue81, 78, 78, (一社)日本歯科理工学会, Mar. 2023, [Peer-reviewed], [Lead author], [Domestic magazines]
Japanese

Development of Autopolymerizing Resin Material with Antimicrobial Properties Using Montmorillonite and Nanoporous Silica
Shuhei Otsubo, Ko Nakanishi, Kakufu Fukukawa, Ryoshun Endo, Seiichiro Yoshida, Aiko Matsumoto, Kumiko Yoshihara, Tsukasa Akasaka, Akira Hasebe, Yasuhiro Yoshida, Yoshiaki Sato
Pharmaceutics, 15, 2, 544, 544, MDPI AG, 06 Feb. 2023, [Peer-reviewed]
Scientific journal, Although autopolymerizing resin offers numerous applications in orthodontic treatment, plaque tends to accumulate between the appliance and the mucosa, which increases the number of microorganisms present. In this study, we added cetylpyridinium chloride (CPC) loaded montmorillonite (Mont) and nanoporous silica (NPS) to autopolymerizing resin (resin-Mont, resin-NPS) and evaluated their drug release capacity, antimicrobial capacity, drug reuptake capacity, mechanical strength, and color tone for the devolvement of autopolymerizing resin with antimicrobial properties. As observed, resin-Mont and resin-NPS were capable of the sustained release of CPC for 14 d, and a higher amount of CPC was released compared to that of resin-CPC. Additionally, resin-Mont and resin-NPS could reuptake CPC. Moreover, the antimicrobial studies demonstrated that resin-Mont and resin-NPS could release effective amounts of CPC against Streptococcus mutans for 14 d and 7 d after reuptake, respectively. Compared to resin-CPC, resin-Mont exhibited a higher sustained release of CPC in all periods, both in the initial sustained release and after reuptake. However, the mechanical strength decreased with the addition of Mont and NPS, with a 36% reduction observed in flexural strength for resin-Mont and 25% for resin-NPS. The application of these results to the resin portion of the orthodontic appliances can prevent bacterial growth on the surface, as well as on the interior, of the appliances and mitigate the inflammation of the mucosa.

Periodontal tissue regeneration by recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
Yuto Yoshino, Hirofumi Miyaji, Erika Nishida, Yukimi Kanemoto, Asako Hamamoto, Akihito Kato, Tsutomu Sugaya, Tsukasa Akasaka
Journal of oral biosciences, 17 Jan. 2023, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVES: Recombinant human collagen peptide (RCP) is a recombinantly created xeno-free biomaterial enriched in arginine-glycine-aspartic acid sequences with good processability whose use for regenerative medicine applications is under investigation. The biocompatibility and osteogenic ability of RCP granules combined with β-tricalcium phosphate (TCP) submicron particles (β-TCP/RCP) were recently demonstrated. In the present study, β-TCP/RCP was implanted into experimental periodontal tissue defects created in beagles to investigate its regenerative effects. METHODS: An RCP solution was lyophilized, granulated, and thermally cross-linked into particles approximately 1 mm in diameter. β-TCP dispersion (1 wt%; 500 μL) was added to 100 mg of RCP granules to form β-TCP/RCP. A three-walled intrabony defect (5 mm × 3 mm × 4 mm) was created on the mesial side of the mandibular first molar and filled with β-TCP/RCP. RESULTS: A micro-computed tomography image analysis performed at 8 weeks postoperative showed a significantly greater amount of new bone after β-TCP/RCP grafting (2.2-fold, P<0.05) than after no grafting. Histological findings showed that the transplanted β-TCP/RCP induced active bone-like tissue formation including tartaric acid-resistant acid phosphatase- and OCN-positive cells as well as bioabsorbability. Ankylosis did not occur, and periostin-positive periodontal ligament-like tissue formation was observed. Histological measurements performed at 8 weeks postoperative revealed that β-TCP/RCP implantation formed 1.7-fold more bone-like tissue and 2.1-fold more periodontal ligament-like tissue than the control condition and significantly suppressed gingival recession and epithelial downgrowth (P<0.05). CONCLUSIONS: β-TCP/RCP implantation promoted bone-like and periodontal ligament-like tissue formation, suggesting its efficacy as a periodontal tissue regenerative material.

温度応答性歯科矯正用解体性接着材の開発　　　　　　　　　　　　　　　
春藤 彩花, 中西 康, 金子 知生, 黒川 考幸, 松本 愛子, 遠藤 諒俊, 赤坂 司, 吉田 靖弘, 佐藤 嘉晃
日本矯正歯科学会大会プログラム・抄録集, 81回, 177, 177, (公社)日本矯正歯科学会, Oct. 2022
Japanese

Extracted tissue-specific atelocollagens have distinctive textural properties.
Sayaka Nakamura, Hiroko Hoshi, Kaito Wakabayashi, Manami Seki, Makoto Watanabe, Momoka Watanabe, Hiroki Inaba, Natsumi Ushijima, Tsukasa Akasaka
Journal of texture studies, 53, 5, 654, 661, Sep. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Food texture is a very important factor for elderly persons, children, and patients who have difficulty swallowing. Collagen and its hydrolysis product, gelatin, are used as ingredients in foods, dietary supplements, and medical materials. In this study, we extracted atelocollagen from nonedible porcine tissues, including ear, nose, and skin, and analyzed the biophysical properties of each tissue. Extracted whole auricle collagen (AEC) showed superior springiness, while only the skin region of auricle collagen (ASC) showed superior hardness, springiness, and brittleness. Body skin collagen showed high hardness but low springiness. In a shear stress test, ASC gels showed high shear strength, and their strains coincided with hardness in a textural examination, while nose and AEC showed low maximum strains. In viscosity, the auricular collagens showed higher viscosity regardless of the region of the ear. Fibril formation in collagen from each tissue and organ varied a great deal in width and morphology. We found that the same type of collagen had a unique texture and viscosity under physiological conditions depending on the tissue or organ of extraction. The results show that the collagen extracted from each organ has a unique texture and unique possibilities to serve as an ingredient in food or supplements.

前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材 骨・歯周組織形成能の評価
金本 佑生実, 宮治 裕史, 西田 絵利香, 宮田 さほり, 眞弓 佳代子, 吉野 友都, 加藤 昭人, 菅谷 勉, 赤坂 司, 大矢根 綾子
日本歯周病学会会誌, 64, 秋季特別, 122, 122, (NPO)日本歯周病学会, Aug. 2022
Japanese

Surface pre-reacted glass-ionomer(S-PRG)ナノフィラー分散液を注水に用いた超音波スケーラーによる歯周炎改善効果
宮治 裕史, 眞弓 佳代子, 金本 佑生実, 岡本 一絵, 浜本 朝子, 加藤 昭人, 菅谷 勉, 赤坂 司, 田中 佐織
日本歯周病学会会誌, 64, 秋季特別, 126, 126, (NPO)日本歯周病学会, Aug. 2022
Japanese

Ultrasonic irrigation of periodontal pocket with surface pre-reacted glass-ionomer (S-PRG) nanofiller dispersion improves periodontal parameters in beagle dogs.
Hirofumi Miyaji, Kayoko Mayumi, Yukimi Kanemoto, Ichie Okamoto, Asako Hamamoto, Akihito Kato, Tsutomu Sugaya, Tsukasa Akasaka, Saori Tanaka
Journal of oral biosciences, 64, 2, 222, 228, Jun. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVES: Surface pre-reacted glass-ionomer (S-PRG) nanofiller, an antibacterial ion-releasing bioactive glass, has been shown to adhere to tooth surfaces and reported to improve inflammatory parameters in experimental periodontitis. In this study, cementum substrate was irrigated ultrasonically with dispersion to examine in-vitro nanofiller adhesion and antibacterial activity. Moreover, periodontal pockets in a beagle dog were ultrasonically irrigated with dispersion to assess periodontal healing. METHODS: The morphology of human cementum irrigated with S-PRG nanofiller dispersion was examined by scanning electron microscopy and energy dispersive X-ray spectrometry. The antibacterial activity of the treated cementum was tested using Actinomyces naeslundii. In addition, experimentally formed periodontal pockets in beagle dog were ultrasonically irrigated with S-PRG nanofiller dispersion. Periodontal parameters (gingival index, bleeding on probing, probing pocket depth, and clinical attachment level) were measured from baseline (0 weeks) through 12 weeks. Moreover, the effects of irrigation with S-PRG nanofiller on changes in periodontal microflora and bone healing were analyzed. RESULTS: After ultrasonic irrigation, S-PRG nanofiller adhered to the cementum and exhibited antibacterial activity. The periodontal parameters were shown to improve following ultrasonic irrigation with S-PRG nanofiller dispersion. Analysis by next-generation sequencing revealed that the ratio of red-complex species decreased in the pockets irrigated with S-PRG nanofiller dispersion. In addition, the S-PRG nanofiller showed the potential to promote bone healing. CONCLUSIONS: Ultrasonic irrigation with S-PRG nanofiller dispersion using an ultrasonic scaler system permitted delivery of the S-PRG nanofiller to the root surface, providing improved parameters in experimental periodontitis and modifying the composition of subgingival periodontal microflora.

Osteoclast formation from mouse bone marrow cells on micro/nano-scale patterned surfaces.
Tsukasa Akasaka, Hiroshi Hayashi, Miho Tamai, Yoshitaka Yoshimura, Yoh-Ichi Tagawa, Hirofumi Miyaji, Ko Nakanishi, Yasuhiro Yoshida
Journal of oral biosciences, 64, 2, 237, 244, Jun. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, OBJECTIVES: Osteoclasts can sense the surface topography of materials. However, it is difficult to identify the structural factors that affect osteoclast formation and its function. Furthermore, we hypothesized that the type of osteoclast precursor cells also affects osteoclastogenesis in the materials. In this study, we investigated the effects of defined micro/nanoscale patterns on osteoclastogenesis from bone marrow cells (BMCs). METHODS: Various cyclo-olefin polymer (COP) patterns were prepared using nanoimprinting. The effects of shape, size, and height of the patterns, and the wettability of the patterned surfaces on osteoclastogenesis from BMCs were evaluated in vitro. RESULTS: Osteoclast formation was promoted on pillars (diameter, 1 μm or 500 nm; height, 500 nm). Notably, osteoclastogenesis from BMCs was better promoted on hydrophobic pillars than on hydrophilic pillars. In contrast, decreased osteoclast formation was observed on the nanopillars (diameter, 100 nm; height, 200 nm). CONCLUSIONS: We demonstrated the promotion of osteoclast formation from BMCs on hydrophobic pillars with diameters of 1 μm and 500 nm. Some cellular behaviors in the patterns were dependent on the type of osteoclast precursor cells. The designed patterns are useful for designing the surface of dental implants or bone replacement materials with a controllable balance between osteoblast and osteoclast activities.

Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure
Tsukasa Akasaka, Miho Tamai, Yoshitaka Yoshimura, Natsumi Ushijima, Shinichiro Numamoto, Atsuro Yokoyama, Hirofumi Miyaji, Ryo Takata, Shuichi Yamagata, Yoshiaki Sato, Ko Nakanishi, Yasuhiro Yoshida
NANO RESEARCH, Jan. 2022, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma- and precursor-assisted biomimetic process.
Yukimi Kanemoto, Hirofumi Miyaji, Erika Nishida, Saori Miyata, Kayoko Mayumi, Yuto Yoshino, Akihito Kato, Tsutomu Sugaya, Tsukasa Akasaka, Arputharaj Joseph Nathanael, Syama Santhakumar, Ayako Oyane
Journal of periodontal research, 57, 1, 205, 218, 16 Nov. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND AND OBJECTIVES: In the treatment of severe periodontal destruction, there is a strong demand for advanced scaffolds that can regenerate periodontal tissues with adequate quality and quantity. Recently, we developed a plasma- and precursor-assisted biomimetic process by which a porous collagen scaffold (CS) could be coated with low-crystalline apatite. The apatite-coated collagen scaffold (Ap-CS) promotes cellular ingrowth within the scaffold compared to CS in rat subcutaneous tissue. In the present study, the osteogenic activity of Ap-CS was characterized by cell culture and rat skull augmentation tests. In addition, the periodontal tissue reconstruction with Ap-CS in a beagle dog was compared to that with CS. METHODS: The plasma- and precursor-assisted biomimetic process was applied to CS to obtain Ap-CS with a low-crystalline apatite coating. The effects of apatite coating on the scaffold characteristics (i.e., surface morphology, water absorption, Ca release, protein adsorption, and enzymatic degradation resistance) were assessed. Cyto-compatibility and the osteogenic properties of Ap-CS and CS were assessed in vitro using preosteoblastic MC3T3-E1 cells. In addition, we performed in vivo studies to evaluate bone augmentation and periodontal tissue reconstruction with Ap-CS and CS in a rat skull and canine furcation lesion, respectively. RESULTS: As previously reported, the plasma- and precursor-assisted biomimetic process generated a low-crystalline apatite layer with a nanoporous structure that uniformly covered the Ap-CS surface. Ap-CS showed significantly higher water absorption, Ca release, lysozyme adsorption, and collagenase resistance than CS. Cell culture experiments revealed that Ap-CS was superior to CS in promoting the osteoblastic differentiation of MC3T3-E1 cells while suppressing their proliferation. Additionally, Ap-CS significantly promoted (compared to CS) the augmentation of the rat skull bone and showed the potential to regenerate alveolar bone in a dog furcation defect. CONCLUSION: Ap-CS fabricated by the plasma- and precursor-assisted biomimetic process provided superior promotion of osteogenic differentiation and bone neoformation compared to CS.

リゾチーム-金ナノクラスター/ローズベンガル複合体の抗菌・抗バイオフィルム光線力学的活性
岡本 一絵, 宮治 裕史, 宮田 さほり, 菅谷 勉, 蔀 佳奈子, 赤坂 司, 齋田 慧, 川崎 英也
日本歯周病学会会誌, 63, 秋季特別, 133, 133, (NPO)日本歯周病学会, Oct. 2021
Japanese

S-PRGナノフィラーを用いた抗菌性歯面コーティングによる臨床パラメーターの改善 イヌ実験的歯周炎における検討
眞弓 佳代子, 宮治 裕史, 宮田 さほり, 西田 絵利香, 降旗 友和, 金本 佑生実, 菅谷 勉, 蔀 佳奈子, 赤坂 司
日本歯周病学会会誌, 63, 秋季特別, 133, 133, (NPO)日本歯周病学会, Oct. 2021
Japanese

Ion Capture and Release Ability of Glass Ionomer Cement Containing Nanoporous Silica Particles with Different Pore and Particle Size
R. Endo, K. Nakanishi, Y. Bando, S. Abe, H. Maruoka, M. Nakamura, T. Akasaka, Y. Yoshida, Y. Sato
Materials, 14, 19, 5742, 5742, MDPI AG, 01 Oct. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, This study prepared glass ionomer cement (GIC) containing nanoporous silica (NPS) (GIC–NPS) at 5 wt% concentrations using 3 types of NPS with different pore and particle sizes and evaluated the differences in their cationic ion capture/release abilities and mechanical properties. The cationic water-soluble dye was used as cationic ion. The test GIC–NPS complexes captured dyes by immersion in 1 wt% dye solutions. All the GIC–NPS complexes released dyes for 28 d, and the amount of dye released from the complexes increased with decreasing pore size; however, the particle size of NPS did not affect the amount of dye released. Additionally, GIC–NPS was able to recharge the dye, and the amount of released the dye by the complexes after recharge was almost identical to the amount released on the first charge. Although not significantly different, the compressive strength of GIC–NPS was slightly greater than that of GIC without NPS regardless of the type of NPS. These results suggest that the degree of capture and release of cationic molecules, such as drugs, can be controlled by optimizing the pore size of NPS without sacrificing its mechanical strength when its content is 5 wt%.

Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates
Ichie Okamoto, Hirofumi Miyaji, Saori Miyata, Kanako Shitomi, Tsutomu Sugaya, Natsumi Ushijima, Tsukasa Akasaka, Satoshi Enya, Satoshi Saita, Hideya Kawasaki
ACS OMEGA, 6, 13, 9279, 9290, Apr. 2021, [Peer-reviewed]
English, Scientific journal

Antibacterial coating of tooth surface with ion-releasing pre-reacted glass-ionomer (S-PRG) nanofillers
Kayoko Mayumi, Hirofumi Miyaji, Saori Miyata, Erika Nishida, Tomokazu Furihata, Yukimi Kanemoto, Tsutomu Sugaya, Kanako Shitomi, Tsukasa Akasaka
HELIYON, 7, 2, Feb. 2021, [Peer-reviewed]
English, Scientific journal

Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles
Erika Nishida, Hirofumi Miyaji, Kanako Shitomi, Tsutomu Sugaya, Tsukasa Akasaka
DENTAL MATERIALS JOURNAL, 40, 1, 157, 164, Feb. 2021, [Peer-reviewed]
English, Scientific journal

Bone forming ability of recombinant human collagen peptide granules applied with beta-tricalcium phosphate fine particles
Tomokazu Furihata, Hirofumi Miyaji, Erika Nishida, Akihito Kato, Saori Miyata, Kanako Shitomi, Kayoko Mayumi, Yukimi Kanemoto, Tsutomu Sugaya, Tsukasa Akasaka
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 108, 7, 3033, 3044, May 2020, [Peer-reviewed]
English, Scientific journal

Size- and Morphology-Controlled Preparation of Surface-Modified Water-Dispersible Fullerene Nanoparticles for Bioapplications.
Oguma H, Seitoku E, Mutoh M, Yoshizawa S, Nakanishi K, Bando Y, Era Y, Kiba T, Saikaew P, Tamai M, Akasaka T, Nakamura M, Kusaka T, Yoshida Y, Sato Y, Sano H, Abe S, Alireza V, Watanabe I, Inoue S, Takada T
Journal of nanoscience and nanotechnology, 20, 5, 2668, 2674, American Scientific Publishers, May 2020, [Peer-reviewed]
Scientific journal, In this study, we investigated water-dispersible surface modification for size- and shape-controlled fullerene nanoparticles (C₆₀P) based on a condensation reaction with di-amino alkane. This modification provided for water dispersibility of C₆₀P and the capability
for secondary modification as well. The resultant C₆₀P particles have several useful physical properties: water-dispersibility for ease of injection; fluorescence for detection and quantification; and a characteristic morphology to assist identification. These properties will widely
extend the applications of these particles, especially into the biological fields of bioimaging and drug delivery.

Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite.
Kanako Shitomi, Hirofumi Miyaji, Saori Miyata, Tsutomu Sugaya, Natsumi Ushijima, Tsukasa Akasaka, Hideya Kawasaki
Photodiagnosis and photodynamic therapy, 30, 101647, 101647, 02 Jan. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Antibacterial photodynamic therapy (a-PDT) is a promising anti-infective technique for generation of singlet oxygen (1O2) to target dental disease. However, conventional organic photosensitizers have some problems for clinical use in terms of cytotoxicity, quenching of a-PDT activity by self-dimerization, and the lack of long-term antibacterial effect. We herein propose silver nanoclusters/rose bengal nanocomposite (AgNCs/RB) as a novel photosensitizer with two primary antibacterial effects: (1) 1O2 generation by irradiated RB and (2) Ag+ ion release from AgNCs. AgNCs/RB irradiated with white light-emitting diode (LED) for a short irradiation time of 1 min significantly decreased the bacterial turbidity of Streptococcus mutans, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans (P < 0.05). In SEM, TEM and LIVE/DEAD staining images, photoexcited AgNCs/RB reduced S. mutans colonization, destroyed the cell membrane, and increased the number of dead cells. The antibacterial efficiency of photoexcited AgNCs/RB was greater than that of AgNCs or RB alone (P < 0.05), suggesting a synergistic effect of 1O2 and Ag + ions from photoexcited AgNCs/RB. By contrast, photoexcited AgNCs/RB did not affect WST-8 and LDH activities and morphology of NIH3T3 mammalian cells, indicating low cytotoxicity. Interestingly, the antibacterial activity of AgNCs/RB on S. mutans was maintained even after the cessation of LED irradiation, indicating a long-term antibacterial effect due to released Ag+ ions. The present AgNCs/RB photosensitizers provide effective synergistic antibacterial effects for dental a-PDT via 1O2 and Ag+ ions coupled with low cytotoxicity.

Morphology and fluorescence of Y2O3:Eu³⁺ nanophosphors and dispersibility in adhesives resins
Shuichi Yamagata, Takaaki Yamamoto, Yoshiaki Sato, Natsumi Ushijima, Tsukasa Akasaka, Yasuhiro Yoshida
Nano Biomedicine, 11, 2, 57, 64, 2020, [Peer-reviewed]
Scientific journal

Fluorescence mechanical properties of Y2O3:Eu³⁺ and Nanophosphor-containing adhesive resins
Shuichi Yamagata, Takaaki Yamamoto, Yoshiaki Sato, Natsumi Ushijima, Tsukasa Akasaka, Yasuhiro Yoshida
Nano Biomedicine, 12, 1, 19, 26, 2020, [Peer-reviewed]
Scientific journal

Proliferation of Saos-2 and Ca9-22 cells on grooved and pillared titanium surfaces.
Kaga N, Akasaka T, Matsuura T, Yokoyama A, Yoshida Y
Bio-medical materials and engineering, 30, 5-6, 559, 567, Jan. 2020, [Peer-reviewed]
English, Scientific journal

Comparative biological assessments of endodontic root canal sealer containing surface pre-reacted glass-ionomer (S-PRG) filler or silica filler.
Hirofumi Miyaji, Kayoko Mayumi, Saori Miyata, Erika Nishida, Kanako Shitomi, Asako Hamamoto, Saori Tanaka, Tsukasa Akasaka
Dental materials journal, 39, 2, 287, 294, 28 Nov. 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Surface pre-reacted glass-ionomer (S-PRG) filler releases several ions, such as fluoride, borate and strontium ions, to exert bioactive effects. We fabricated an endodontic root canal sealer containing S-PRG fillers (S-PRG sealer) and then evaluated the antibacterial and anti-inflammatory properties of S-PRG sealer compared with sealer containing conventional silica fillers (silica sealer). Antibacterial tests showed that S-PRG sealer significantly reduced the turbidity of Enterococcus faecalis compared with silica sealer. Implantation of S-PRG or silica sealer blocks in rat subcutaneous tissue showed that S-PRG sealer decreased the proinflammatory response compared with silica sealer at 10 days post-implantation. In addition, immunostaining revealed that infiltration of CD68- and peroxidase-positive cells around the S-PRG sealer was significantly lower than that in silica sealer. Therefore, it was suggested that S-PRG sealer exhibits antibacterial and anti-inflammatory effects.

Fusion of RAW 264.7 macrophage cells on micro-scale fine pillar patterns　　　　　　　　　　　　　　　
J. Honma, T. Akasaka, M. Tamai, Y. Yoshimura, T. Taira, H. Miyaji, S. Yamagata, Y. Sato, Y. Yoshida
Digest Journal of Nanomaterials and Biostructures, 13, 4, 1123, 1131, Dec. 2018, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Optical and Chromatic Evaluation of Fluorescent Orthodontic Bonding Materials Doped with a Europium β-diketonate Complex
Aiko MATSUMOTO, Shuichi YAMAGATA, Shigeaki ABE, Tsukasa AKASAKA, Yasuhiro YOSHIDA, Junichiro IIDA
Nano Biomedicine, 10, 1, 3, 8, Aug. 2018, [Peer-reviewed]

Evaluation of Tissue Behavior on Three-dimensional Collagen Scaffold Coated with Carbon Nanotubes and β-tricalcium Phosphate Nanoparticles
Hirofumi MIYAJI, Shusuke MURAKAMI, Erika NISHIDA, Tsukasa AKASAKA, Bunshi FUGETSU, Junko UMEDA, Katsuyoshi KONDOH, Tadashi IIZUKA, Tsutomu SUGAYA
Journal of Oral Tissue Engineering, 15, 3, 123, 130, May 2018, [Peer-reviewed]

Effect of a nano-scale fine hole pattern on the differentiation of RAW264.7 Cells into osteoclasts
R. Takata, T. Akasaka, M. Tamai, Y. Yoshimura, T. Taira, H. Miyaji, Y. Tagawa, S. Yamagata, J. Iida, Y. Yoshida
Digest Journal of Nanomaterials and Biostructures, 13, 2, 451, 458, Apr. 2018, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Near-infrared Irradiation and Graphene Oxide Film Fabricated on Dentin Surface Exhibit Photothermal and Antibacterial Effects
Nagao Keishi, Miyaji Hirofumi, Nishida Erika, Akasaka Tsukasa, Miyata Saori, Shitomi Kanako, Mayumi Kayoko, Kato Akihito, Sugaya Tsutomu
Journal of Oral Hygiene & Health, 6, 1, 231, 231, OMICS International, 10 Jan. 2018, [Peer-reviewed]
English, Scientific journal

Fabrication of light-curing type fluorescent orthodontic bonding materials using europium-ß-diketonate complex
Shuichi Yamagata, Takaaki Yamamoto, Yoshiaki Sato, Shigeaki Abe, Tsukasa Akasaka, Yasuhiro Yoshida
Nano Biomedicine, 10, 2, 53, 60, 2018, [Peer-reviewed]
Scientific journal

Y2O3:Eu3+ phosphors by homogeneous precipitation and subsequent calcination at varied temperatures for orthodontic adhesives
Shuichi Yamagata, Takaaki Yamamoto, Yoshiaki Sato, Natsumi Ushijima, Shigeaki Abe, Tsukasa Akasaka, Yasuhiro Yoshida, Junichiro Iida
Nano Biomedicine, 9, 1, 15, 22, National Institute of Informatics, 2018, [Peer-reviewed]
English, Scientific journal

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants.
Reika Makita, Tsukasa Akasaka, Seiichi Tamagawa, Yasuhiro Yoshida, Saori Miyata, Hirofumi Miyaji, Tsutomu Sugaya
Beilstein journal of nanotechnology, 9, 1735, 1754, 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, Background: Collagen is a basic component of the periodontium and plays an important role in the function of the periodontal unit. Therefore, coating with collagen/gelatin has been applied to enable dental implants to positively interact with peri-implant tissues. Although the micro/nanoscale topography is an important property of the surface of dental implants, smaller collagen/gelatin surface patterns have not been sufficiently developed. Furthermore, only few reports on the behavior of cells on gelatin surfaces with different patterns and sizes exist. In this study, we developed micro/nanometer-scaled gelatin surfaces using genipin crosslinking, with the aim of understanding the use of patterning in surface modification of dental implants. Results: Grooves, holes, and pillars, with widths or diameters of 2 µm, 1 µm, or 500 nm were fabricated using a combination of molding and genipin crosslinking of gelatin. The stability of the different gelatin patterns could be controlled by the degree of genipin crosslinking. The gelatin patterns at 20 mM concentration of genipin and 41% crosslinking maintained a stable, patterned shape for at least 14 days in a cell culture medium. A cell morphology study showed that the cells on groves were aligned along the direction of the grooves. In contrast, the cells on pillars and holes exhibited randomly elongated filopodia. The vinculin spots of the cells were observed on the top of ridges and pillars or the upper surface of holes. The results of a cell attachment assay showed that the number of surface-attached cells increased with increasing patterning of the gelatin surface. Unlike the cell attachment assay, the results of a cell proliferation assay showed that Saos-2 cells prefer grooves with diameters of approximately 2 µm and 1 µm and pillars with diameters of 1 µm and heights of 500 nm. The number of cells on pillars with heights of 2 µm was larger than those of the other gelatin surface patterns tested. Conclusion: These data support that a detailed design of the gelatin surface pattern can control both cell attachment and proliferation of Saos-2 cells. Thus, gelatin surfaces patterned using genipin crosslinking are now an available option for biocompatible material patterning.

Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds.
Shusuke Murakami, Hirofumi Miyaji, Erika Nishida, Kohei Kawamoto, Saori Miyata, Hiroko Takita, Tsukasa Akasaka, Bunshi Fugetsu, Toshihiko Iwanaga, Hiromi Hongo, Norio Amizuka, Tsutomu Sugaya, Masamitsu Kawanami
Dental materials journal, 36, 5, 573, 583, 26 Sep. 2017, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (β-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of β-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% β-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with β-TCP nanoparticles applied increased with β-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area of TCP10 was 7.7-fold greater than that of non-treated scaffold. In contrast, TCP25 consistently exhibited adverse biological effects. These results suggest that the application dose of β-TCP nanoparticles affects the scaffold bioproperties; consequently, the bone conductive ability of TCP10 was remarkable.

Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation
Saori Miyata, Hirofumi Miyaji, Hideya Kawasaki, Erika Nishida, Kanako Shitomi, Tsukasa Akasaka, Saori Tanaka, Tadashi Iizuka, Tsutomu Sugaya
Biology, Engineering and Medicine, 2, 4, 1, 4, Open Access Text, Sep. 2017, [Peer-reviewed]
English, Scientific journal, The development of photosensitizers and light sources has enabled the use of antimicrobial photodynamic therapy (aPDT) in various dental therapies. In the presentstudy, we compared the antibacterial and cytotoxic effects of Au clusters photoexcited by blue and white LED irradiation. We fabricated novel photosensitizers,captopril-protected gold (Capt-Au) clusters and lysozyme-stabilized gold (Lyz-Au) clusters, for aPDT. Au clusters were then photoexcited by two kinds of lightsources, blue high-power and white low-power light-emitting diodes (LEDs). Since white LED contains a wide spectrum of light (400–750 nm), white LED wouldbe relevant for aPDT even if using a low-power source.The turbidity and viability of Streptococcus mutans were assessed following application of Capt-Au clusters (500 μg/mL) or Lyz-Au clusters (1,000 μg/mL) photoexcitedby a blue high-power LED (1,000 mW/cm2) or white low-power LED (80 mW/cm2). In addition, the cytotoxicity of Au clusters and LED irradiation was evaluatedin NIH3T3 and MC3T3-E1 cells.Au clusters photoexcited by the white low-power LED equally decreased the turbidity and viability of S. mutans compared with blue high-power LED. However,Au clusters photoexcited by white LED irradiation caused decreased cytotoxicity in mammalian cells compared with those photoexcited by blue LED irradiation. Inconclusion, white LEDs possess biosafe properties for aPDT using Au clusters.

Interfacial interactions of bioadhesive materials with human hard tissues
Yoshida, Yasuhiro, Inoue, Satoshi, Tamai, Miho, Abe, Shigeaki, Akasaka, Tsukasa, Yoshihara, Kumiko, Meerbeek, Bart Van
Hokkaido Journal of Dental Science, 38, 86, 98, 北海道歯学会, Sep. 2017, [Peer-reviewed]
English, Scientific journal, Recently, in dental practice, tooth reconstruction can be performed using dental adhesive technology following either a "glass-ionomer", an "etch-and-rinse" or a "self-etch" approach. Glass-ionomer cements are well-known to possess an auto-adhesive capability without requiring any surface pre-treatment. The fundamental bonding mechanism of resin-based materials to enamel and dentin is essentially based on an exchange process, in which minerals removed from the dental hard tissues are replaced by resin monomers. These resin monomers, upon polymerization become micro-mechanically interlocked in the created porosities. Besides micro-mechanical interlocking through hybridization, an additional chemical interaction between functional monomers/polymers and tooth substrate components has been found to be important. In this review, we focus on how the chemical interaction of the biomaterial-hard tissue interface can improve bond durability, especially using chemical analytical techniques.

Cell adhesion and alignment of human osteoblasts and human gingival fibroblasts on micro/nano-grooved gelatin sheet
Naoyuki Kaga, Tsukasa Akasaka, Rumi Horiuchi, Yasuhiro Yoshida, Atsuro Yokoyama
Digest Journal of Nanomaterials and Biostructures, 12, 2, 431, 440, May 2017, [Peer-reviewed]
English, Scientific journal

SUBMICRO-PATTERNING OF CURABLE DENTAL MATERIALS BY MOLDING METHODS: A SCREENING TRIAL
T. Akasaka, H. Miyaji, T. Imamura, N. Kaga, A. Yokoyama, Y. Yoshida
DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES, 12, 2, 281, 292, Apr. 2017, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Effect of micro/nano-patterned surfaces on cell adhesion of Ca9-22 cells
Naoyuki Kaga, Rumi Horiuchi, Atsuro Yokoyama, Tsukasa Akasaka, Yasuhiro Yoshida
e-Journal of Surface Science and Nanotechnology, 15, 1, 6, Surface Science Society of Japan, 11 Feb. 2017, [Peer-reviewed]
English, International conference proceedings

Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation.
Saori Miyata, Hirofumi Miyaji, Hideya Kawasaki, Masaki Yamamoto, Erika Nishida, Hiroko Takita, Tsukasa Akasaka, Natsumi Ushijima, Toshihiko Iwanaga, Tsutomu Sugaya
International journal of nanomedicine, 12, 2703, 2716, 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal, Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au25(Capt)18) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen (1O2). Accordingly, the antimicrobial and cytotoxic effects of Au25(Capt)18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. 1O2 generation of Au25(Capt)18 clusters under blue LED irradiation (420-460 nm) was detected by a methotrexate (MTX) probe. The antimicrobial effects of photoexcited Au clusters (0, 5, 50, and 500 μg/mL) on oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis, were assessed by morphological observations and bacterial growth experiments. Cytotoxicity testing of Au clusters and blue LED irradiation was then performed against NIH3T3 and MC3T3-E1 cells. In addition, the biological performance of Au clusters (500 μg/mL) was compared to an organic dye photosensitizer, methylene blue (MB; 10 and 100 μg/mL). We confirmed the 1O2 generation ability of Au25(Capt)18 clusters through the fluorescence spectra of oxidized MTX. Successful application of photoexcited Au clusters to aPDT was demonstrated by dose-dependent decreases in the turbidity of oral bacterial cells. Morphological observation revealed that application of Au clusters stimulated destruction of bacterial cell walls and inhibited biofilm formation. Aggregation of Au clusters around bacterial cells was fluorescently observed. However, photoexcited Au clusters did not negatively affect the adhesion, spreading, and proliferation of mammalian cells, particularly at lower doses. In addition, application of Au clusters demonstrated significantly better cytocompatibility compared to MB. We found that a combination of Au25(Capt)18 clusters and blue LED irradiation exhibited good antimicrobial effects through 1O2 generation and biosafe characteristics, which is desirable for aPDT in dentistry.

Effect of protein adsorption on alignment of human gingival fibroblasts on grooved composite resin
Tsukasa Akasaka, Takuya Imamura, Hirofumi Miyaji, Naoyuki Kaga, Atsuro Yokoyama, Yasuhiro Yoshida
e-Journal of Surface Science and Nanotechnology, 14, 225, 230, Surface Science Society of Japan, 10 Dec. 2016, [Peer-reviewed], [Lead author, Corresponding author]
English, International conference proceedings

Adhesion of human osteoblast-like cells (Saos-2 cells) on micro/nanopatterned structures sputter-coated with titanium
Kaga N, Akasaka T, Horiuchi R, Yoshida Y, Yokoyama A
Nano Biomedicine, 8, 2, 74, 82, Dec. 2016, [Peer-reviewed]
English, Scientific journal

Adhesion of osteoblast-like cells (Saos-2) on micro-/submicro-patterned apatite scaffolds fabricated with apatite cement paste by micro-molding
Akasaka T, Miyaji H, Kaga N, Yokoyama A, Abe A, Yoshida Y
Nano Biomedicine, 8, 2, 112, 122, Dec. 2016, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Evaluation of Fluorescent Orthodontic Adhesives Containing Y2O3:Eu3+ Particles
Shuichi YAMAGATA, Yoshiaki SATO, Takaaki YAMAMOTO, Shigeaki ABE, Tsukasa AKASAKA, Yasuhiro YOSHIDA, Junichiro IIDA
Nano Biomedicine, 8, 1, 35, 40, Oct. 2016, [Peer-reviewed]
English, Scientific journal

Evaluation of a Gliding Arc Discharge Non-thermal Atmospheric Pressure Plasma Device for Orthodontic Bonding
Shuichi YAMAGATA, Takaaki YAMAMOTO, Yoshiaki SATO, Shigeaki ABE, Tsukasa AKASAKA, Yasuhiro YOSHIDA, Junichiro IIDA
Nano Biomedicine, 8, 1, 57, 63, Oct. 2016, [Peer-reviewed]
English, Scientific journal

Development of Poly(L-lactic acid)/Organically Modified Montmorillonite Nanocomposites for the Fabrication of Orthodontic Anchoring Screws
Ko Nakanishi, Shuichi Yamagata, Tsukasa Akasaka, Shigeaki Abe, Yasuhiro Yoshida, Junichiro Iida
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 16, 9, 9292, 9297, Sep. 2016, [Peer-reviewed]
English, Scientific journal

Estimation of Biocompatibility of Nano-Sized Ceramic Particles with Osteoblasts, Osteosarcomas and Hepatocytes by Static and Time-Lapse Observation
Shigeaki Abe, Eri Seitoku, Nobuki Lwadera, Yusuke Hamba, Shuichi Yamagata, Tsukasa Akasaka, Teruo Kusaka, Satoshi Lnoue, Yasutaka Yawaka, Junichiro Lida, Hidehiko Sano, Tetsu Yonezawa, Yasuhiro Yoshida
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 12, 3, 472, 480, Mar. 2016, [Peer-reviewed]
English, Scientific journal

Cytocompatiblity of ceramic nano particles to various types of cells
Shigeaki Abe, Nobuki Iwadera, Mami Mutoh, Tomohiko Katsurayama, Shino Morimoto, Ko Nakanishi, Tsukasa Akasaka, Satoshi Inoue, Yasutaka Yawaka, Junichiro Iida, Yasuhiko Yoshida
Key Engineering Materials, 696, 234, 237, Trans Tech Publications Ltd, 2016, [Peer-reviewed]
English, International conference proceedings

Biological Response to Nanostructure of Carbon Nanotube/titanium Composite Surfaces
Erika NISHIDA, Hirofumi MIYAJI, Junko UMEDA, Katsuyoshi KONDOH, Hiroko TAKITA, Izumi KANAYAMA, Saori TANAKA, Akihito KATO, Bunshi FUGETSU, Tsukasa AKASAKA, Masamitsu KAWANAMI
Nano Biomedicine, 7, 1, 11, 20, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Preparation of a poly(lactic acid)/montmorillonite nanocomposite
NAKANISHI KO, YAMAGATA SHUICHI, AKASAKA TSUKASA, ABE SHIGEAKI, YOSHIDA YASUHIRO, IIDA JUNICHIRO
Key Eng Mater, 631, 151, 155, 2015
English

Platinum on Nanodiamond: A Promising Prodrug Conjugated with Stealth Polyglycerol, Targeting Peptide and Acid-Responsive Antitumor Drug
Li Zhao, Yong-Hong Xu, Hongmei Qin, Shigeaki Abe, Tsukasa Akasaka, Tokuhiro Chano, Fumio Watari, Takahide Kimura, Naoki Komatsu, Xiao Chen
ADVANCED FUNCTIONAL MATERIALS, 24, 34, 5348, 5357, Sep. 2014, [Peer-reviewed]
English, Scientific journal

Polyglycerol-coated nanodiamond as a macrophage-evading platform for selective drug delivery in cancer cells
Li Zhao, Yong-Hong Xu, Tsukasa Akasaka, Shigeaki Abe, Naoki Komatsu, Fumio Watari, Xiao Chen
BIOMATERIALS, 35, 20, 5393, 5406, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Influence of MWCNTs to Myocardial Contraction Rhythms on Differentiation of ES-D3 Cells in Three-dimensional Culture
Koichi IMAI, Tsubasa SHIRAI, Fumio WATARI, Tsukasa AKASAKA, Tetsunari NISHIKAWA, Tomoharu OKAMURA, Akio TANAKA, Kazuhiko SUESE, Fumiya OGAWA, Yoshitomo HONDA, Hirofumi SAWAI, Hiromasa TAKASHIMA
Nano Biomedicine, 6, 1, 27, 34, Jun. 2014, [Peer-reviewed]
English, Scientific journal

Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering
Erika Nishida, Hirofumi Miyaji, Hiroko Takita, Izumi Kanayama, Maiko Tsuji, Tsukasa Akasaka, Tsutomu Sugaya, Ryuji Sakagami, Masamitsu Kawanami
JAPANESE JOURNAL OF APPLIED PHYSICS, 53, 6, 06JD04, Jun. 2014, [Peer-reviewed]
English, Scientific journal

Preparation and biocompatibilities of luminescent europium-doped yttria and titania nanoparticles
Shigeaki Abe, Yusuke Hamba, Atsushi Hyono, Shuichi Yamagata, Motohiro Uo, Junichiro Iida, Takayuki Kiba, Akihiro Murayama, Tsukasa Akasaka, Fumio Watari
JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 122, 1423, 216, 221, Mar. 2014, [Peer-reviewed]
English, Scientific journal

Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold
Yoshinori Kuboki, Toshitake Furusawa, Masaaki Sato, Yongkun Sun, Hidero Unuma, Shigeaki Abe, Ryuichi Fujisawa, Tsukasa Akasaka, Fumio Watari, Hiroko Takita, Rachel Sammons
BIO-MEDICAL MATERIALS AND ENGINEERING, 24, 3, 1539, 1548, 2014, [Peer-reviewed]
English, Scientific journal

Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide
Izumi Kanayama, Hirofumi Miyaji, Hiroko Takita, Erika Nishida, Maiko Tsuji, Bunshi Fugetsu, Ling Sun, Kana Inoue, A. Sako Ibara, Tsukasa Akasaka, Tsutomu Sugaya, Masamitsu Kawanami
INTERNATIONAL JOURNAL OF NANOMEDICINE, 9, 3363, 3373, 2014, [Peer-reviewed]
English, Scientific journal

Application of Fourier Transform for Analysis of Surface Topographic Properties of Dental Zirconia
Naoyoshi TARUMI, Tsukasa AKASAKA, Fumio WATARI
Nano Biomedicine, 5, 2, 85, 94, Dec. 2013, [Peer-reviewed]
English, Scientific journal

Preparation and Properties of Fluorescent Orthodontic Adhesives Containing Y2O3:Eu3+ Particles
Yusuke HAMBA, Shuichi YAMAGATA, Tsukasa AKASAKA, Motohiro UO, Junichiro IIDA, Fumio WATARI
Nano Biomedicine, 5, 2, 75, 84, Dec. 2013, [Peer-reviewed]
English, Scientific journal

An Attempt to Study of the Embryotoxicity by the Diamond Particles of Dental Diamond Points with the Embryonic Stem Cell Test
Koichi IMAI, Fumio WATARI, Tsukasa AKASAKA, Kazuhiko SUESE, Fumiya OGAWA, Hirofumi SAWAI, Hiromasa TAKASHIMA
Nano Biomedicine, 5, 2, 104, 108, Dec. 2013, [Peer-reviewed]
English, Scientific journal

The effects of the coating of anodized titanium with multi-walled carbon nanotuves on bone formation.
Inoue S, Uo M, Sakairi M, Hirata E, Lee M.H, Bae T.S, Akasaka T, Watari F, Yokoyama A
Key Engineering Materials, 529-530, 621, 624, Nov. 2013, [Peer-reviewed]
English, Scientific journal

An In Vitro Evaluation of the Biological Effects of Carbon Nanotube-Coated Dental Zirconia
Wen Kou, Tsukasa Akasaka, Fumio Watari, Göran Sjögren
ISRN Dentistry, 2013, 296727, 296727, Jul. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal, THE PURPOSE OF THIS STUDY IS TO EVALUATE FUNCTIONALIZED MULTIWALLED CARBON NANOTUBES (FMWCNTS) AS A POTENTIAL COATING MATERIAL FOR DENTAL ZIRCONIA FROM A BIOLOGICAL PERSPECTIVE: its effect on cell proliferation, viability, morphology, and the attachment of an osteoblast-like cell. Osteoblast-like (Saos-2) cells were seeded on uncoated and fMWCNT-coated zirconia discs and in culture dishes that served as controls. The seeding density was 10(4) cells/cm(2), and the cells were cultured for 6 days. Cell viability, proliferation and attachment of the Saos-2 cells were studied. The results showed that Saos-2 cells were well attached to both the uncoated and the fMWCNT-coated zirconia discs. Cell viability and proliferation on the fMWCNT-coated zirconia discs were almost the same as for the control discs. Better cell attachment was seen on the fMWCNT-coated than on the uncoated zirconia discs. In conclusion, fMWCNTs seem to be a promising coating material for zirconia-based ceramic surfaces to increase the roughness and thereby enhance the osseointegration of zirconia implants.

An Evaluation of Fluorescent Orthodontic Adhesives Containing Eu-doped ZnO at Room Temperature
Shuichi YAMAGATA, Hiroshi IWASAKI, Yusuke HAMBA, Ko NAKANISHI, Natsumi USHIJIMA, Shigeaki ABE, Tsukasa AKASAKA, Fumio WATARI, Junichiro IIDA
Nano Biomedicine, 5, 1, 31, 38, Jun. 2013, [Peer-reviewed]
English, Scientific journal

Adsorption behavior of albumin and other proteins on carbon nanotubes studied by chromatography
M. Morikawa, Y. Kuboki, T. Akasaka, S. Abe, H. Takita, F. Watari
Key Engineering Materials, 529-530, 1, 615, 620, 2013, [Peer-reviewed]
English, International conference proceedings

Influence of micro-/nano- particles on osteoblast-like cells: A static and time lapse observation
Nobuki Iwadera, Shigeaki Abe, Tsukasa Akasaka, Yasutaka Yawaka, Fumio Watari
BIOCERAMICS 24, 529-530, 374, +, 2013, [Peer-reviewed]
English, International conference proceedings

Biocompatibility and biodistribution of several nano-sized ceramics particles
Shigeaki Abe, Yusaku Hamba, Nobuki Iwadera, Tsukasa Akasaka, Shuichi Yamagata, Yasutaka Yawaka, Junichiro Iida, Motohiro Uo, Tetsu Yonezawa, Fumio Watari
BIOCERAMICS 24, 529-530, 625, +, 2013, [Peer-reviewed]
English, International conference proceedings

Murine macrophage RAW264.7 cells response for the carbon nanotubes immobilized on substrate
Tsukasa Akasaka, Shigeaki Abe, Fumio Watari
Key Engineering Materials, 529-530, 1, 379, 384, 2013, [Lead author, Corresponding author]
English, International conference proceedings

Applications of micro/nano patterned bio-materials in dental field
T. Akasaka, Fumio Watari
Hokkaido Journal of Dental Science, 32, 2, 189, 191, 2013, [Peer-reviewed], [Lead author, Corresponding author]
Japanese, Scientific journal

Investigation of biodistribution behavior of platinum particles in mice: Correlation between inductively coupled plasma-atomic emission spectroscopy and X-ray scanning analytical microscopy
Shigeaki Abe, Chika Koyama, Mami Mutoh, Tsukasa Akasaka, Motohiro Uo, Fumio Watari
APPLIED SURFACE SCIENCE, 262, 125, 128, Dec. 2012, [Peer-reviewed]
English, Scientific journal

Morphology, size distribution and elemental composition of several dental debris
Shigeaki Abe, Nobuki Iwadera, Mitsue Esaki, Ken-Ichi Aoyama, Tsukasa Akasaka, Motohiro Uo, Manabu Morita, Yasutaka Yawaka, Fumio Watari
APPLIED SURFACE SCIENCE, 262, 248, 252, Dec. 2012, [Peer-reviewed]
English, Scientific journal

Evaluation of Adhesion between Material and Epithelium using a Three-dimensional Human Epidermal Model
Kazunori FURUHASHI, Tsukasa AKASAKA, Yoshimasa KITAGAWA, Fumio WATARI
Nano Biomedicine, 4, 2, 76, 84, Dec. 2012, [Peer-reviewed]
English, Scientific journal

The effect of enhancing the hydrophobicity of OMMT on the characteristics of PMMA/OMMT nanocomposites
Shuichi Yamagata, Yusuke Hamba, Tsukasa Akasaka, Natsumi Ushijima, Motohiro Uo, Junichiro Iida, Fumio Watari
APPLIED SURFACE SCIENCE, 262, 56, 59, Dec. 2012, [Peer-reviewed]
English, Scientific journal

Carbon nanotube-coating accelerated cell adhesion and proliferation on poly (L-lactide)
Eri Hirata, Tsukasa Akasaka, Motohiro Uo, Hiroko Takita, Fumio Watari, Atsuro Yokoyama
APPLIED SURFACE SCIENCE, 262, 24, 27, Dec. 2012, [Peer-reviewed]
English, Scientific journal

Deposition of TiC film on titanium for abrasion resistant implant material by ion-enhanced triode plasma CVD
Yuhe Zhu, Wei Wang, Xingya Jia, Tsukasa Akasaka, Susan Liao, Fumio Watari
APPLIED SURFACE SCIENCE, 262, 156, 158, Dec. 2012, [Peer-reviewed]
English, Scientific journal

Phytotoxicity of multi-walled carbon nanotubes assessed by selected plant species in the seedling stage
Parvin Begum, Refi Ikhtiari, Bunshi Fugetsu, Makoto Matsuoka, Tsukasa Akasaka, Fumio Watari
APPLIED SURFACE SCIENCE, 262, 120, 124, Dec. 2012, [Peer-reviewed]
English, Scientific journal

In vitro study of cell differentiation by two type mouse embryo stem cells on mono- and multilayer nanocarbon tubes
Koichi Imai, Tsukasa Akasaka, Fumio Watari, Akito Tanoue, Kazuaki Nakamura, Kazuhiko Suese, Hiromasa Takashima, Tetsunari Nishikawa, Akio Tanaka, Shoji Takeda
APPLIED SURFACE SCIENCE, 258, 22, 8444, 8447, Sep. 2012, [Peer-reviewed]
English, Scientific journal

Carbonate Ion Induced Calcification Using Biomimetic Enzymes with Active Reaction Center of Carbonc Anhydrase
Shigeaki ABE, Minoru YOHDA, Aki NISHIYAMA, Mitsunori NAKATANI, Yuhei FUJII, Arisa KUSUHARA, Emi YAMATOYA, Tomoki ISHIDA, Tsukasa AKASAKA, Motohiro UO, Fumio WATARI, Daisuke HAYASHI, Tomoya TAKADA
Nano Biomedicine, 4, 1, 29, 34, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Effect of Pretreatments to Carbon Nanotubes on Protein Adsorption
Mai MORIKAWA, Yoshinori KUBOKI, Tsukasa AKASAKA, Sigeaki ABE, Fumio WATARI
Nano Biomedicine, 4, 1, 35, 41, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Introduction of Rare-Earth-Element- Containing ZnO Nanoparticles into Orthodontic Adhesives
Shuichi YAMAGATA, Yusuke HAMBA, Ko NAKANISHI, Shigeaki ABE, Tsukasa AKASAKA, Natsumi USHIJIMA, Motohiro UO, Junichiro IIDA, Fumio WATARI
Nano Biomedicine, 4, 1, 11, 17, Jun. 2012, [Peer-reviewed]
English, Scientific journal

Carbon nanotube-coated silicone as a flexible and electrically conductive biomedical material
Makoto Matsuoka, Tsukasa Akasaka, Yasunori Totsuka, Fumio Watari
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 32, 3, 574, 580, Apr. 2012, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Comparison of biodistribution and biocompatibility of gelatin-coated copper nanoparticles and naked copper oxide nanoparticles
Shigeaki Abe, Nobuki Iwadera, Takashi Narushima, Yoshiki Uchida, Motohiro Uo, Tsukasa Akasaka, Yasutaka Yawaka, Fumio Watari, Tetsu Yonezawa
e-Journal of Surface Science and Nanotechnology, 10, 33, 37, 17 Mar. 2012, [Peer-reviewed]
English, International conference proceedings

Internal Diffusion of Biocompatible Polymer-Coated Inorganic Nanoparticles in Mice
S. Abe, A. Sakaki, T. Narushima, Y. Uchida, T. Akasaka, M. Uo, T. Yonezawa, F. Watari
Key Engneering Materials, 493-494, 752, 756, 2012
English, International conference proceedings

Interaction between titanium and phosphoproteins revealed by chromatography column packed with titanium beads
Yoshinori Kuboki, Toshitake Furusawa, Masaaki Sato, Yongkun Sun, Hidero Unuma, Ryuichi Fujisawa, Shigeaki Abe, Tsukasa Akasaka, Fumio Watari, Hiroko Takita, Rachel Sammons
BIO-MEDICAL MATERIALS AND ENGINEERING, 22, 5, 283, 288, 2012, [Peer-reviewed]
English, Scientific journal

Biodistribution of Aqueous Suspensions of Carbon Nanotubes in Mice and Their Biocompatibility
Shigeaki Abe, Sachiko Itoh, Daisuke Hayashi, Toshihisa Kobayashi, Takayuki Kiba, Tsukasa Akasaka, Motohiro Uo, Yasutaka Yawaka, Shin-Ichiro Sato, Fumio Watari, Tomoya Takada
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 12, 1, 700, 706, Jan. 2012, [Peer-reviewed]
English, Scientific journal

Biodistribution and Biocompatibility of Poly(lactic acid)-coated SiO2 Particle
Shigeaki ABE, Akiko SASAKI, Nobuki IWADERA, Tsukasa AKASAKA, Motohiro UO, FumioI WATARI
Nano Biomedicine, 3, 2, 300, 305, Nov. 2011, [Peer-reviewed]
English, Scientific journal

Optical and Mechanical Properties of Poly (methyl methacrylate) / Montmorillonite Nanocomposites
Shuichi YAMAGATA, Yusuke HAMBA, Tsukasa AKASAKA, Motohiro UO, Junichiro IIDA, Fumio WATARI
Nano Biomedicine, 3, 1, 217, 223, Nano Biomedical Society, Aug. 2011, [Peer-reviewed]
English, Scientific journal, Transparent poly (methyl methacrylate)/organically modified montmorillonite (PMMA/OMMT) nanocomposites were fabricated using a solution intercalation method. OMMT was modified with quaternary alkylammonium ions. The haze values, which are the ratios of diffuse transmittance to total light transmittance via the nanocomposites, gradually increased with an increasing amount of OMMT; however, the deterioration of total light transmittance was maintained within a narrow range. X-ray diffraction patterns showed that a peak regarded as a (001) d-spacing or a second peak corresponding to (002) plane was shifted toward lower 2θ values indicating the penetration of the PMMA polymer chains into the interlayer regions of OMMT. Board-shaped specimens used for the flexural tests were fabricated by compression molding at 230°C. The specimens were sufficiently transparent for their use as esthetic orthodontic wires. The flexural modulus of the PMMA/OMMT nanocomposites increased favorably with an increasing amount of OMMT. It is reasonable to consider that the addition of OMMT to the nanocomposites effectively improves the stiffness of PMMA without seriously deteriorating its flexibility.

Versatile Surface Modification by Carbon Nanotubes Through an Amide-bond Formation
Shigeaki ABE, Katsutoshi NAKAYAMA, Hirohisa KOBAYASHI, Takayuki KIBA, Tsukasa AKASAKA, Shin-ichiro SATO, Motohiro UO, Fumio WATARI, Tomoya TAKADA
Nano Biomedicine, 3, 1, 208, 216, Nano Biomedical Society, Aug. 2011, [Peer-reviewed]
English, Scientific journal, There is considerable investigation into the applications of carbon nanotubes because of their valuable properties. In this study, our goal was to develop several carbon nanotube (CNT)-coated substrates through an amide-bonding process. We prepared polycarboxylated multi-walled CNTs via a carboxylation reaction and then CNT derivatives were tethered to amino groups presented on a titanium, gold or glass substrate in the presence of a condensation agent. The obtained substrates were characterized with a Raman microscope, an atomic force microscope (AFM) and a scanning electron microscope (SEM). Raman mapping images indicated that the CNTs existed on the substrate surface homogeneously, and AFM and SEM observation revealed that the CNTs coating the surfaces had a three-dimensional network nanostructure. The CNTs remained on the surface even after ultrasonication treatment in organic solvent. However, they were removed from the substrate surface after soaking in NaOH aqueous solution. This result suggested that the CNTs were tethered to the substrate through amide bonding.

Study of In Vitro Embryotoxicity Potential by Two Type Nano Titanium Dioxide
Koichi IMAI, Tsukasa AKASAKA, Fumio WATARI, Akito TANOUE, Kazuaki NAKAMURA, Kazuhiko SUESE, Hiromasa TAKASHIMA, Tetsunari NISHIKAWA, Akio TANAKA, Shoji TAKEDA
Nano Biomedicine, 3, 1, 224, 230, Nano Biomedical Society, Aug. 2011, [Peer-reviewed]
English, Scientific journal, Nano and submicron titanium dioxides are widely used as ingredients of sun screen. Thus, its reproductive and developmental toxicities in humans are of concern. We used two kinds of nano titanium dioxides, involving different production methods and surface coatings. The embryotoxicities of these nano titanium dioxides were determined based on inhibitory effects on the cardiomyocyte differentiation of mouse Embryonic Stem cells. As ultrafine titanium dioxide, titanium dioxide (particle diameter, 0.03-0.05 μm) produced by the sintering method (T1) and that (particle diame-ter, 0.01-0.02 μm) produced by the wet method (T2) were used. T2 may cause little developmental toxicity because it has no potent inhibitory effects on cardiomyocyte differentiation. However, T1 should be further investigated.

Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering
Eri Hirata, Motohiro Uo, Hiroko Takita, Tsukasa Akasaka, Fumio Watari, Atsuro Yokoyama
CARBON, 49, 10, 3284, 3291, Aug. 2011, [Peer-reviewed]
English, Scientific journal

Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes
Tsukasa Akasaka, Atsuro Yokoyama, Makoto Matsuoka, Takeshi Hashimoto, Fumio Watari
CARBON, 49, 7, 2287, 2299, Jun. 2011, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Toxicity evaluations of various carbon nanomaterials
Motohiro Uo, Tsukasa Akasaka, Fumio Watari, Yoshinori Sato, Kazuyuki Tohji
DENTAL MATERIALS JOURNAL, 30, 3, 245, 263, May 2011, [Peer-reviewed]
English, Scientific journal

Observation of a 3D network nano-structure of carbon nanotubes scaffold for cultivation
Shigeaki Abe, Kosuke Ishikawa, Atsushi Hyono, Hirohisa Kobayashi, Takayuki Kiba, Tsukasa Akasaka, Motohiro Uo, Yasutaka Yawaka, Shin-Ichiro Sato, Tetsu Yonezawa, Fumio Watari
e-Journal of Surface Science and Nanotechnology, 9, 80, 84, 05 Mar. 2011, [Peer-reviewed]
English, International conference proceedings

Observation of Internal Distribution Behavior of Micro/Nano-Sized Ceramics and Metal Particles in Mice
Shigeaki Abe, Nobuki Iwadera, Mami Mutoh, Chika Koyama, Mitsue Esaki, Tsukasa Akasaka, Motohiro Uo, Manabu Morita, Yoshinori Kuboki, Koichi Haneda, Yasutaka Yawaka, Fumio Uchida, Tetsu Yonezawa, Fumio Watari
Bioceramics Development and Applications, 1, D110134, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Development of a novel transparent substrate coated by carbon nanotubes through covalent bonding
Shigeaki Abe, Katsutoshi Nakayama, Daisuke Hayashi, Tsukasa Akasaka, Motohiro Uo, Fumio Watari, Tomoya Takada
9TH INTERNATIONAL CONFERENCE ON NANO-MOLECULAR ELECTRONICS, 14, 147, 151, 2011, [Peer-reviewed]
English, International conference proceedings

Internal distribution of micro- / Nano-sized inorganic particles and their cytocompatibility
Shigeaki Abe, Nobuki Iwadera, Mitsue Esaki, Ikuhiro Kida, Mami Mutoh, Tsukasa Akasaka, Motohiro Uo, Yasutaka Yawaka, Manabu Morita, Koichi Haneda, Tetsu Yonezawa, Fumio Watari
IOP Conference Series: Materials Science and Engineering, 18, 13, 192013, 2011, [Peer-reviewed]
English, International conference proceedings

Application of Imogolite, Almino-Silicate Nanotube, as Scaffold for the Mineralization of Osteoblasts
Kosuke Ishikawa, Tsukasa Akasaka, Shigeaki Abe, Yasutaka Yawaka, Masaya Suzuki, Fumio Watari
Bioceramics Development and Applications, 1, D110133, Jan. 2011, [Peer-reviewed]
English, Scientific journal

Culture of ES Cells and Mesenchymal Stem Cells on Carbon Nanotube Scaffolds
Hiromi KITAHARA, Yoshinori KUBOKI, Hiroko TAKITA, Tsukasa AKASAKA, Fumio WATARI, Nobuo INOUE
Nano Biomedicine, 2, 2, 81, 92, Nano Biomedical Society, Dec. 2010, [Peer-reviewed]
English, Scientific journal, Cell proliferations on carbon nanotube scaffolds were investigated for the embryonic stem cells (ES cells) of mouse and the bone marrow-derived mesenchymal stem cells (MSC) of rat. Although ES cells were well attached and proliferated on gelatin-coated plastic dishes, they did not attach on the CNT-coated scaffold, and formed the spheroids growing in the culture medium. For MSC, they attached on the CNT-coated scaffold and grew to form mineralized particles as on the plastic dishes of control. The growth rate of the MSC was lower than control but showed the higher expression of alkaline phosphatase activity per cell in 2 weeks and degree of mineralization per cell for 3 and 4 weeks.

Strong adhesion of Saos-2 cells to multi-walled carbon nanotubes
Makoto Matsuoka, Tsukasa Akasaka, Yasunori Totsuka, Fumio Watari
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, 173, 1-3, 182, 186, Oct. 2010, [Peer-reviewed]
English, Scientific journal

The bactericidal effect of carbon nanotube/agar composites irradiated with near-infrared light on Streptococcus mutans
Tsukasa Akasaka, Makoto Matsuoka, Takeshi Hashimoto, Shigeaki Abe, Motohiro Uo, Fumio Watari
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, 173, 1-3, 187, 190, Oct. 2010, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Photophysical characterization of cumarin-doped poly (lactic acid) microparticles and visualization of the biodistribution
Shigeaki Abe, Takayuki Kiba, Kiyotada Hosokawa, Satoru Nitobe, Takashi Hirota, Hirohisa Kobayashi, Tsukasa Akasaka, Motohiro Uo, Yoshinori Kuboki, Shin-Ichiro Sato, Fumio Watari, Iosif D. Rosca
JOURNAL OF ELECTRON SPECTROSCOPY AND RELATED PHENOMENA, 181, 2-3, 181, 185, Aug. 2010, [Peer-reviewed]
English, Scientific journal

Chromatography of carbon nanotubes separated albumin from other serum proteins: a method for direct analysis of their interactions
Yoshinori Kuboki, Takamitu Koshikawa, Hiroko Takita, Ryuichi Fujisawa, Min-ho Lee, Shigeaki Abe, Tsukasa Akasaka, Motohiro Uo, Fumio Watari, Rachel Sammons
DENTAL MATERIALS JOURNAL, 29, 4, 369, 373, Jul. 2010, [Peer-reviewed]
English, Scientific journal

Internal distribution of micro-/nano-sized ceramics and metals particles in mice
Shigeaki Abe, Ikuhiro Kida, Mitsue Esaki, Nobuki Iwadera, Mami Mutoh, Chika Koyama, Tsukasa Akasaka, Motohiro Uo, Yoshinori Kuboki, Manabu Morita, Yoshinori Sato, Koichi Haneda, Tetsu Yonezawa, Balachandran Jeyadevan, Kazuyuki Tohji, Fumio Watari
JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 118, 1378, 525, 529, Jun. 2010, [Peer-reviewed]
English, Scientific journal

In Vitro Study of Cell Differentiation by Mouse Embryo Stem Cells on Nanocarbon Tubes
Koichi IMAI, Kazuhiko SUESE, Tsukasa AKASAKA, Fumio WATARI, Shoji TAKEDA
Nano Biomedicine, 2, 1, 47, 51, Nano Biomedical Society, Jun. 2010, [Peer-reviewed]
English, Scientific journal, We examined the influence of culture methods with mono- and multilayer nanocarbon tubes (SWCNT and MWCNT, respectively) on the differentiation of mouse embryonic stem (ES) cells using a cellular differentiation technique for the embryonic stem cell test (EST). On differentiation assay, beating was noted in 72, 35, and 60% of the cells in the control, SWCNT, and MWCNT groups, respectively. In addition, there was no influence on cytotoxicity based on the LDH level. In this study, the MWCNT did not influence the differentiation of ES cells, but the SWCNT markedly inhibited it, confirming that the differentiation of ES cells depends on the material surface structure.

3D collagen scaffolds coated with multiwalled carbon nanotubes: Initial cell attachment to internal surface
Eri Hirata, Motohiro Uo, Yoshinobu Nodasaka, Hiroko Takita, Natsumi Ushijima, Tsukasa Akasaka, Fumio Watari, Atsuro Yokoyama
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 93B, 2, 544, 550, May 2010, [Peer-reviewed]
English, Scientific journal

Thin films of single-walled carbon nanotubes promote human osteoblastic cells (Saos-2) proliferation in low serum concentrations
Tsukasa Akasaka, Atsuro Yokoyama, Makoto Matsuoka, Takeshi Hashimoto, Fumio Watari
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 30, 3, 391, 399, Apr. 2010, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

High Functional Expression of Osteoblasts on Imogolite, Aluminosilicate Nanotubes
Kosuke Ishikawa, Tsukasa Akasaka, Yasutaka Yawaka, Fumio Watari
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 6, 1, 59, 65, Feb. 2010, [Peer-reviewed]
English, Scientific journal

Various nanotube scaffolds for cell proliferation
F. Watari, T. Akasaka, K. Ishikawa, M. Matsuoka, E. Hirata, N. Terada, A. Yokoyama, M. Uo, S. Itoh, Y. Yawaka, M. Suzuki, N. Takashi, Y. Totsuka, Y. Kitagawa, S. Abe, I. D. Rosca, Y. Kuboki, Y. Bando
MULTISCALE, MULTIFUNCTIONAL AND FUNCTIONALLY GRADED MATERIALS, 631-632, 181, +, 2010, [Peer-reviewed]
English, International conference proceedings

Preparation and Properties of Thermoplastic-resin-based Nanocomposites for the Development of Esthetic Orthodontic Wires
Shuichi YAMAGATA, Tsukasa AKASAKA, Motohiro UO, Natsumi USHIJIMA, Yoshinobu NODASAKA, Junichiro IIDA, Fumio WATARI
Nano Biomedicine, 1, 2, 151, 158, Dec. 2009, [Peer-reviewed]
English, Scientific journal

Physical Properties of Aluminosilicate Nanotubes, Imogolite, as Scaffold and Effect on Osteoblastic Mineralization
Kosuke ISHIKAWA, Tsukasa AKASAKA, Yoshinobu NODASAKA, Natsumi USHIJIMA, Masayuki KAGA, Shigeaki ABE, Motohiro UO, Yasutaka YAWAKA, Masaya SUZUKI, Fumio WATARI
Nano Biomedicine, 1, 2, 109, 120, Dec. 2009, [Peer-reviewed]
English, Scientific journal

Synthesis and Characterization of a Water-soluble Multi-walled Carbon Nanotube and Its Biodistribution in Mice
Shigeaki ABE, Daisuke HAYASHI, Tsukasa AkASAKA, Motohiro UO, Yoshinori KUBOKI, Fumio WATARI, Tomoya TAKADA
Nano Biomedicine, 1, 2, 143, 150, Dec. 2009, [Peer-reviewed]
English, Scientific journal

Effect of carbon nanotubes on cellular functions in vitro
Xiaoming Li, Hong Gao, Motohiro Uo, Yoshinori Sato, Tsukasa Akasaka, Qingling Feng, Fuzhai Cui, Xinhui Liu, Fumio Watari
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 91A, 1, 132, 139, Oct. 2009, [Peer-reviewed]
English, Scientific journal

Time-Dependence and Visualization of TiO(2) and Pt Particle Biodistribution in Mice
Shigeaki Abe, Chika Koyama, Motohiro Uo, Tsukasa Akasaka, Yoshinobu Kuboki, Fumio Watari
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 9, 8, 4988, 4991, Aug. 2009, [Peer-reviewed]
English, Scientific journal

In Vitro Evaluation of Porous Poly(L-Lactic Acid) Scaffold Reinforced by Chitin Fibers
Xiaoming Li, Xinhui Liu, Wei Dong, Qingling Feng, Fuzhai Cui, Motohiro Uo, Tsukasa Akasaka, Fumio Watari
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 90B, 2, 503, 509, Aug. 2009, [Peer-reviewed]
English, Scientific journal

Development of a 3D Collagen Scaffold Coated With Multiwalled Carbon Nanotubes
Eri Hirata, Motohiro Uo, Hiroko Takita, Tsukasa Akasaka, Fumio Watari, Atsuro Yokoyama
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 90B, 2, 629, 634, Aug. 2009, [Peer-reviewed]
English, Scientific journal

Proliferation of osteoblast cells on nanotubes
F. Watari, T. Akasaka, Xiaoming Li, M. Uo, A. Yokoyama
Frontiers of Materials Science in China, 3, 2, 169, 173, Jun. 2009, [Peer-reviewed]
English, International conference proceedings

Observation of Biodistribution of Indium-Tin Oxide Nanoparticles in Mice
Shigeaki ABE, Tetsu YONEZAWA, Tsukasa AKASAKA, Motohiro UO, Fumio UCHIDA, Fumio WATARI
Nano Biomedicine, 1, 1, 70, 74, Jun. 2009, [Peer-reviewed]
English, Scientific journal

Material nanosizing effect on living organisms: non-specific, biointeractive, physical size effects
Fumio Watari, Noriyuki Takashi, Atsuro Yokoyama, Motohiro Uo, Tsukasa Akasaka, Yoshinori Sato, Shigeaki Abe, Yasunori Totsuka, Kazuyuki Tohji
JOURNAL OF THE ROYAL SOCIETY INTERFACE, 6, 3, S371, S388, Jun. 2009, [Peer-reviewed]
English, Scientific journal

Maturation of osteoblast-like SaoS2 induced by carbon nanotubes
Xiaoming Li, Hong Gao, Motohiro Uo, Yoshinori Sato, Tsukasa Akasaka, Shigeaki Abe, Qingling Feng, Fuzhai Cui, Fumio Watari
BIOMEDICAL MATERIALS, 4, 1, 015995, Feb. 2009, [Peer-reviewed]
English, Scientific journal

Capture of bacteria by flexible carbon nanotubes
Tsukasa Akasaka, Fumio Watari
ACTA BIOMATERIALIA, 5, 2, 607, 612, Feb. 2009, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Adhesion of human osteoblast-like cells (Saos-2) to carbon nanotube sheets
Tsukasa Akasaka, Atsuro Yokoyama, Makoto Matsuoka, Takeshi Hashimoto, Shigeaki Abe, Motohiro Uo, Fumio Watari
BIO-MEDICAL MATERIALS AND ENGINEERING, 19, 2-3, 147, 153, 2009, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Improvement in cell proliferation on silicone rubber by carbon nanotube coating
Makoto Matsuoka, Tsukasa Akasaka, Takeshi Hashimoto, Yasunori Totsuka, Fumio Watari
BIO-MEDICAL MATERIALS AND ENGINEERING, 19, 2-3, 155, 162, 2009, [Peer-reviewed]
English, Scientific journal

Modification of the dentin surface by using carbon nanotubes
Tsukasa Akasaka, Keiko Nakata, Motohiro Uo, Fumio Watari
BIO-MEDICAL MATERIALS AND ENGINEERING, 19, 2-3, 179, 185, 2009, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Visualization of invasion into the body and internal diffusion of nanoparticles
F. Watari, S. Abe, I. D. Rosca, A. Yokoyama, M. Uo, T. Akasaka, N. Takashi, Y. Totsuka, E. Hirata, M. Matsuoka, K. Ishikawa, S. Itoh, Y. Yawaka
BIOCERAMICS 21, 396-398, 569, 572, 2009, [Peer-reviewed]
English, International conference proceedings

Multiwalled carbon nanotube monoliths prepared by spark plasma sintering (SPS) and their mechanical properties
Motohiro Uo, Tomoka Hasegawa, Tsukasa Akasaka, Isao Tanaka, Fuminori Munekane, Mamoru Omori, Hisamichi Kimura, Reiko Nakatomi, Kohei Soga, Yasuo Kogo, Fumio Watari
BIO-MEDICAL MATERIALS AND ENGINEERING, 19, 1, 11, 17, 2009, [Peer-reviewed]
English, Scientific journal

Biodistribution imaging of magnetic particles in mice: X-ray scanning analytical microscopy and magnetic resonance imaging
Shigeaki Abe, Ikuhiro Kida, Mitsue Esaki, Tsukasa Akasaka, Motohiro Uo, Toshiaki Hosono, Yoshinori Sato, Balachandran Jeyadevan, Yoshinori Kuboki, Manabu Morita, Kazuyuki Tohji, Fumio Watari
BIO-MEDICAL MATERIALS AND ENGINEERING, 19, 2-3, 213, 220, 2009, [Peer-reviewed]
English, Scientific journal

In vivo internal diffusion of several inorganic microparticles through oral administration
Shigeaki Abe, Chila Koyama, Mitsue Esaki, Tsukasa Akasaka, Motohiro Uo, Yoshinori Kuboki, Manabu Morita, Fumio Watari
BIO-MEDICAL MATERIALS AND ENGINEERING, 19, 2-3, 221, 229, 2009, [Peer-reviewed]
English, Scientific journal

Multiwalled carbon nanotube coating on titanium
Michiko Terada, Shigeaki Abe, Tsukasa Akasaka, Motohiro Uo, Yoshimasa Kitagawa, Fumio Watari
BIO-MEDICAL MATERIALS AND ENGINEERING, 19, 1, 45, 52, 2009, [Peer-reviewed]
English, Scientific journal

Development of a multiwalled carbon nanotube coated collagen dish
Michiko Terada, Shigeaki Abe, Tsukasa Akasaka, Motohiro Uo, Yoshimasa Kitagawa, Fumio Watari
Dental Materials Journal, 28, 1, 82, 88, 2009, [Peer-reviewed]
English, Scientific journal

XAFS Analysis of Ti and Ni Dissolution from Pure Ti, Ni-Ti Alloy, and SUS304 in Soft Tissues
Motohiro Uo, Kiyotaka Asakura, Kazuchika Tamura, Yasunori Totsuka, Shigeaki Abe, Tsukasa Akasaka, Fumio Watari
CHEMISTRY LETTERS, 37, 9, 958, 959, Sep. 2008, [Peer-reviewed]
English, Scientific journal

Preparation and characteristics of a binderless carbon nanotube monolith and its biocompatibility
Wei Wang, Atsuro Yokoyama, Susan Liao, Mamoru Omori, Yuhe Zhu, Motohiro Uo, Tsukasa Akasaka, Fumio Watari
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 28, 7, 1082, 1086, Aug. 2008, [Peer-reviewed]
English, Scientific journal

Biochemical and Pathological Responses of Cells and Tissue to Micro‐ and Nanoparticles from Titanium and other Materials
Fumio Watari, Kazuchika Tamura, Atsruro Yokoyama, Kenichiro Shibata, Tsukasa Akasaka, Bunshi Fugetsu, Kiyotaka Asakura, Motohiro Uo, Yasunori Totsuka, Yoshinori Sato, Kazuyuki Tohji
Biological Aspects and Structure Formation, 127, 144, Mar. 2008, [Peer-reviewed]
English, Research society, ISBN: 9783527619443

Determination of administered organic/inorganic particles in mice　　　　　　　　　　　　　　　
Shigeaki Abe, Chika Koyama, Mitsue Esaki, Ikuhiro Kida, Iosif Rosca, Tsukasa Akasaka, Motohiro Uo, Manabu Morita, Yoshinori Sato, Balachandran Jeyadevan, Yoshinori Kuboki, Kazuyuki Tohji, Fumio Watari
8th World Biomaterials Congress 2008, 1, 460, 2008
English, International conference proceedings

Carbohydrate coating of carbon nanotubes for biological recognition
Tsukasa Akasaka, Fumio Watari
FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES, 16, 2, 114, 125, 2008, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Behavior of in vitro, in vivo and internal motion of micro/nano particles of titanium, titanium oxides and others
Fumio Watari, Shigeaki Abe, Chika Koyama, Atsuro Yokoyama, Tukasa Akasaka, Motohiro Uo, Makoto Matsuoka, Yasunori Totsuka, Mitsue Esaki, Manabu Morita, Tetsu Yonezawa
JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 116, 1349, 1, 5, Jan. 2008, [Peer-reviewed]
English, Scientific journal

The degradation of the three layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane in vitro
Susan Liao, Furnio Watari, Yuhe Zhu, Motohiro Uo, Tsukasa Akasaka, Wei Wang, Guofu Xu, Fuzhai Cui
DENTAL MATERIALS, 23, 9, 1120, 1128, Sep. 2007, [Peer-reviewed]
English, Scientific journal

Carbon nanotubes as scaffolds for cell culture and effect on cellular functions
Naofumi Aoki, Tsukasa Akasaka, Fumio Watari, Atsuro Yokoyama
DENTAL MATERIALS JOURNAL, 26, 2, 178, 185, Mar. 2007, [Peer-reviewed]
English, Scientific journal

X-ray absorption fine structure (XAFS) analysis of titanium-implanted soft tissue
Motohiro Uo, Kiyotaka Asakura, Atsuro Yokoyama, Makoto Ishikawa, Kazuchika Tamura, Yasunori Totsuka, Tsukasa Akasaka, Fumio Watari
DENTAL MATERIALS JOURNAL, 26, 2, 268, 273, Mar. 2007, [Peer-reviewed]
English, Scientific journal

The purity and thermal stability in air of metal-encapsulating carbon nanocapsules (MECNCs)
Motohiro Uo, Hanako Kachi, Tsukasa Akasaka, Fumio Watari, Yoshinori Sato, Kenichi Motomiya, Kazuyuki Tohji
FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES, 15, 4, 303, 309, 2007, [Peer-reviewed]
English, Scientific journal

Inhibition of growth of Streptococcus mutans with artificial glyco-polymer
赤坂司
DE, 161, 161, 35, 36, (一社)日本歯科理工学会, 2007, [Lead author, Corresponding author]
Japanese

Carbon nanotubes deposited on titanium implant for osteoblast attachment
Naofumi Aoki, Atsuro Yokoyama, Yoshinobu Nodasaka, Tsukasa Akasaka, Motohiro Uo, Yoshinori Sato, Kazuyuki Tohji, Fumio Watari
Journal of Bionanoscience, 1, 1, 14, 16, 2007, [Peer-reviewed]
English, Scientific journal

Strikingly extended morphology of cells grown on carbon nanotubes
Naofumi Aoki, Atsuro Yokoyama, Yoshinobu Nodasaka, Tsukasa Akasaka, Motohiro Uo, Yoshinori Sato, Kazuyuki Tohji, Fumio Watari
CHEMISTRY LETTERS, 35, 5, 508, 509, May 2006
English, Scientific journal

Apatite formation on carbon nanotubes
T Akasaka, F Watari, Y Sato, K Tohji
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 26, 4, 675, 678, May 2006, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

X-ray absorption fine structure (XAFS) analyses of Ni species trapped in graphene sheet of carbon nanofibers
Y. Sato, M. Ohtsubo, B. Jeyadevan, K. Tohji, K. Motomiya, R. Hatakeyama, G. Yamamoto, M. Omori, T. Hashida, K. Tamura, T. Akasaka, M. Uo, A. Yokoyama, F. Watari
Physical Review, B, 73, 144103-1, 144103-11, Apr. 2006, [Peer-reviewed]
English, Scientific journal

Human neutrophils reaction to the biodegraded nano-hydroxyapatite/collagen and nano-hydroxyapatite/collagen/poly(L-lactic acid) composites
S Liao, K Tamura, YH Zhu, W Wang, M Uo, T Akasaka, FZ Cui, F Watari
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 76A, 4, 820, 825, Mar. 2006
English, Scientific journal

Functions of Sugar chains in oral cavity　　　　　　　　　　　　　　　
Tsukasa Akasaka, Fumio Watari
The journal of dental engineering, 159, 26, 28, 2006, [Lead author, Corresponding author]

Glyco-technology and Dentistry　　　　　　　　　　　　　　　
Tsukasa Akasaka, Motohiro Uo, Fumio Watari
Hokkaido Journal of Dental Science, 27, 1, 41, 44, 2006, [Lead author, Corresponding author]

Cell Culture on a Carbon Nanotube Scaffold
Naofurni Aoki, Atsuro Yokoyama, Yoshinobu Nodasaka, Tsukasa Akasaka, Motohiro Uo, Yoshinori Sato, Kazuyuki Tohji, Furnio Watari
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 1, 4, 402, 405, Dec. 2005, [Peer-reviewed]
English, Scientific journal

A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration
S Liao, W Wang, M Uo, S Ohkawa, T Akasaka, K Tamura, FZ Cui, F Watari
BIOMATERIALS, 26, 36, 7564, 7571, Dec. 2005, [Peer-reviewed]
English, Scientific journal

Oxidation of multiwalled carbon nanotubes by nitric acid
ID Rosca, F Watari, M Uo, T Akaska
CARBON, 43, 15, 3124, 3131, Dec. 2005
English, Scientific journal

Size separation of carbon nanotubes for biomedical application　　　　　　　　　　　　　　　
Kazuyuki Tohji, Yoshinori Sato, Atsuro Yokoyama, Ken-ichiro Shibata, Shin-ichi Ogino, Kazuchika Tamura, Tsukasa Akasaka, Motohiro Uo, Kenichi Motomiya, Balachandran Jeyadevan, Rikizo. Hatakeyama, Fumio Watari
Proceedings of International Symposium on EcoTopia Science 2005, 1, 273, 276, 08 Aug. 2005, [Peer-reviewed]
English, International conference proceedings

Water-soluble hat-stacked carbon nanofibers for biomedical applications　　　　　　　　　　　　　　　
Yoshinori Sato, Ken-ichiro Shibata, Fugetsu Bunshi, Atsuro Yokoyama, Shin-ichi Ogino, Kazuchika Tamura, Tsukasa Akasaka, Motohiro Uo, Kenichi Motomiya, Balachandran Jeyadevan, Rikizo. Hatakeyama, Fumio Watari, Kazuyuki Tohji
Proceedings of International Symposium on EcoTopia Science 2005, 1, 277, 280, 08 Aug. 2005, [Peer-reviewed]
English, International conference proceedings

Strict preparation and evaluation of water-soluble hatstacked carbon nanofibers for biomedical application and their high biocompatibility: influence of nanofiber-surface functional groups on cytotoxicity
Y Sato, K Shibata, H Kataoka, S Ogino, F Bunshi, A Yokoyama, K Tamura, T Akasaka, M Uo, K Motomiya, B Jeyadevan, R Hatakeyama, F Watari, K Tohji
MOLECULAR BIOSYSTEMS, 1, 2, 142, 145, Jul. 2005, [Peer-reviewed]
English, Scientific journal

Influence of length on cytotoxicity of multi-walled carbon nanotubes against human acute monocytic leukemia cell line THP-I in vitro and subcutaneous tissue of rats in vivo
Y Sato, A Yokoyama, K Shibata, Y Akimoto, S Ogino, Y Nodasaka, T Kohgo, K Tamura, T Akasaka, M Uo, K Motomiya, B Jeyadevan, M Ishiguro, R Hatakeyama, F Watari, K Tohji
MOLECULAR BIOSYSTEMS, 1, 2, 176, 182, Jul. 2005, [Peer-reviewed]
English, Scientific journal

Analysis of titanium dental implants surrounding soft tissue using X-ray absorption fine structure (XAFS) analysis
M Uo, K Asakura, A Yokoyama, K Tamura, Y Totsuka, T Akasaka, F Watari
CHEMISTRY LETTERS, 34, 6, 776, 777, Jun. 2005, [Peer-reviewed]
English, Scientific journal

Nano-architecture on carbon nanotube surface by biomimetic coating
T Akasaka, F Watari
CHEMISTRY LETTERS, 34, 6, 826, 827, Jun. 2005, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Preparation and Biocompatibility of Sintered Carbon Nanotube Disk　　　　　　　　　　　　　　　
M. Ohtsubo, Y. Sato, B. Jeyadevan, K. Tohji, R. Hatakeyama, G. Yamamoto, M. Ohmori, T. Hashida, K. Tamura, T. Akasaka, M. Uo, A. Yokoyama, F. Watari
Fullerenes and Nanotubes –Materials for the New Chemical Frontier-Fullerenes (The Electrochemical Society, Inc.), PV2004-12, 243, 248, 01 Mar. 2005, [Invited]
English, International conference proceedings

Biological behavior of hat-stacked carbon nanofibers in the subcutaneous tissue in rats
A Yokoyama, Y Sato, Y Nodasaka, S Yamamoto, T Kawasaki, M Shindoh, T Kohgo, T Akasaka, M Uo, F Watari, K Tohji
NANO LETTERS, 5, 1, 157, 161, Jan. 2005, [Peer-reviewed]
English, Scientific journal

A novel sintered multi-walled carbon nanotubes bone material by spark plasma sintering method
W. Wang, A. Yokoyama, M. Omori, S. Liao, Y. Zhu, T. Akasaka, M. Uo, F. Watari
International Journal of Oral and Maxillofacial Surgery, 34, 1, 83, 2005, [Peer-reviewed]
English, Scientific journal

Caged multiwalled carbon nanotubes as the adsorbents for affinity-based elimination of ionic dyes
B Fugetsu, S Satoh, T Shiba, T Mizutani, YB Lin, N Terui, Y Nodasaka, K Sasa, K Shimizu, R Akasaka, M Shindoh, KI Shibata, A Yokoyama, M Mori, K Tanaka, Y Sato, K Tohji, S Tanaka, N Nishi, F Watari
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 38, 24, 6890, 6896, Dec. 2004, [Peer-reviewed]
English, Scientific journal

Distortion of laser welded titanium plates
K Iwasaki, S Ohkawa, JD Rosca, M Uo, T Akasaka, F Watari
DENTAL MATERIALS JOURNAL, 23, 4, 593, 599, Dec. 2004, [Peer-reviewed]
English, Scientific journal

Three-dimensional topographic scanning electron microscope and Raman spectroscopic analyses of the irradiation effect on teeth by Nd : YAG, Er : YAG, and CO2 lasers
MK Yamada, M Uo, S Ohkawa, T Akasaka, F Watari
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 71B, 1, 7, 15, Oct. 2004, [Peer-reviewed]
English, Scientific journal

Laser welding of titanium and dental precious alloys
K Iwasaki, S Ohkawa, M Uo, T Akasaka, F Watari
MATERIALS TRANSACTIONS, 45, 4, 1140, 1146, Apr. 2004
English, Scientific journal

Non-contact surface morphology analysis of CO2 laser-irradiated teeth by scanning electron microscope and confocal laser scanning, microscope
MK Yamada, M Uo, S Ohkawa, T Akasaka, A Watari
MATERIALS TRANSACTIONS, 45, 4, 1033, 1040, Apr. 2004
English, Scientific journal

Effects of micro/nano particle size on cell function and morphology
K Tamura, N Takashi, T Akasaka, ID Roska, M Uo, Y Totsuka, F Watari
BIOCERAMICS, VOL 16, 254-2, 919, 922, 2004, [Peer-reviewed]
English, Scientific journal

Preparation of carbon nanotubes for biomedical applications: Size-separation and their biocompatibilities　　　　　　　　　　　　　　　
K. Tohji, Y. Akimoto, Y. Sato, B. Jeyadevan, K. Tamura, T. Akasaka, M. Uo, A. Yokoyama, K. - I. Shibata, F. Watari
Proceedings - Electrochemical Society, PV 2004-12, 178, 183, 2004, [Peer-reviewed]
English, Scientific journal

Biocompatibility of carbon nanotube disk
Y. Sato, M. Ohtsubo, B. Jeyadevan, K. Tohji, K. Motomiya, R. Hatakeyama, G. Yamamoto, M. Omori, T. Hashida, K. Tamura, T. Akasaka, M. Uo, A. Yokoyama, F. Watari
Proc. SPIE, 623, 627, Jan. 2004, [Peer-reviewed]
English, International conference proceedings

Size separation of carbon nanotubes for biomedical applic applications
Y. Sato, Y. Akimoto, B. Jeyadevan, K. Motomiya, R. Hatakeyama, K. Tamura, T. Akasaka, M. Uo, A. Yokoyama, K. Shibata, F. Watari, K. Tohji
Proc. SPIE, 13, 17, Jan. 2004, [Peer-reviewed]
English, International conference proceedings

Synthesis of DNA-Carbohydrate Conjugate via Diazocoupling: A New Class of Modified DNA with Enhanced Stability and Lectin-Recognition Ability
Kazunori Matsuura, Tsukasa Akasaka, Miki Hibino, Kazukiyo Kobayashi
Chem.Lett, 28, 3, 247, 248, May 2003, [Peer-reviewed]
English, Scientific journal

Fine Particles and Bioreaction　　　　　　　　　　　　　　　
亘理文夫, 田村一央, 高師則行, 宇尾基弘, 赤坂 司, 戸塚靖典, 古月文志, 佐藤義倫, 田路和幸
ナノ学会会報, 2, 1, 34, 37, 2003, [Peer-reviewed]
Japanese, Scientific journal

Structural analysis of arabinogalactan from Japanese larch in Hokkaido and chemicalfunctionalization of arabinogalactans
T. Akasaka, Y. Maekawa, S. Nisimura
Cellul Commun, 9, 1, 13, 17, Mar. 2002, [Peer-reviewed], [Lead author, Corresponding author]
Japanese, Scientific journal

Transformation from block-type to graft-type oligonucleotide-glycopolymer conjugates by self-organization with half-sliding complementary oligonucleotides and their lectin recognition
T Akasaka, K Matsuura, K Kobayashi
BIOCONJUGATE CHEMISTRY, 12, 5, 776, 785, Sep. 2001, [Peer-reviewed], [Lead author]
English, Scientific journal

Facile synthesis of stable and lectin-recognizable DNA-carbohydrate conjugates via diazo coupling
K Matsuura, T Akasaka, M Hibino, K Kobayashi
BIOCONJUGATE CHEMISTRY, 11, 2, 202, 211, Mar. 2000, [Peer-reviewed]
English, Scientific journal

Conjugation of plasmid DNAs with lactose via diazocoupling enhances resistance to restriction enzymes and acquires binding affinity to galactose-specific lectin
T Akasaka, K Matsuura, N Emi, K Kobayashi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 260, 2, 323, 328, Jul. 1999, [Peer-reviewed], [Lead author]
English, Scientific journal

Synthesis of polymethacrylate derivatives having sulfated maltoheptaose side chains with anti-HIV activities
T Yoshida, T Akasaka, Y Choi, K Hattori, B Yu, T Mimura, Y Kaneko, H Nakashima, E Aragaki, M Premanathan, N Yamamoto, T Uryu
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 37, 6, 789, 800, Mar. 1999, [Peer-reviewed]
English, Scientific journal

高齢者の口腔感染症の防止と介護者の負担軽減に資するCPC徐放・リチャージ義歯の開発
中西康, 赤坂司, 吉田靖弘, 日本歯科医学会誌, 44, 2025

Bone Regeneration and Reconstruction Using Non-animal Derived Bioabsorbable Polymers
吉田靖弘, 中西康, 赤坂司, 日本口腔インプラント学会誌, 37, 2, 2024

ヒトI型コラーゲン様リコンビナントペプチド/β-三リン酸カルシウム骨補填材による歯周組織再生効果
吉野友都, 宮治裕史, 西田絵利香, 金本佑生実, 浜本朝子, 加藤昭人, 菅谷勉, 赤坂司, 日本歯周病学会会誌(Web), 65, 2023

温度応答性歯科矯正用解体性接着材の開発　　　　　　　　　　　　　　　
春藤 彩花, 中西 康, 金子 知生, 黒川 考幸, 松本 愛子, 遠藤 諒俊, 赤坂 司, 吉田 靖弘, 佐藤 嘉晃, 日本矯正歯科学会大会プログラム・抄録集, 81回, 177, 177, Oct. 2022
(公社)日本矯正歯科学会, Japanese

前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材 骨・歯周組織形成能の評価
金本 佑生実, 宮治 裕史, 西田 絵利香, 宮田 さほり, 眞弓 佳代子, 吉野 友都, 加藤 昭人, 菅谷 勉, 赤坂 司, 大矢根 綾子, 日本歯周病学会会誌, 64, 秋季特別, 122, 122, Aug. 2022
(NPO)日本歯周病学会, Japanese

Surface pre-reacted glass-ionomer(S-PRG)ナノフィラー分散液を注水に用いた超音波スケーラーによる歯周炎改善効果
宮治 裕史, 眞弓 佳代子, 金本 佑生実, 岡本 一絵, 浜本 朝子, 加藤 昭人, 菅谷 勉, 赤坂 司, 田中 佐織, 日本歯周病学会会誌, 64, 秋季特別, 126, 126, Aug. 2022
(NPO)日本歯周病学会, Japanese

微細構造が破骨細胞への分化に与える影響
赤坂 司, 中西 康, 吉田 靖弘, 北海道外科雑誌, 67, 1, 95, 95, Jun. 2022
北海道外科学会, Japanese

微細構造が破骨細胞への分化に与える影響　　　　　　　　　　　　　　　
赤坂 司, 中西 康, 吉田 靖弘, 北海道外科雑誌, 67, 1, 95, 95, Jun. 2022
北海道外科学会, Japanese

パイロジェンフリー・非動物由来生体吸収性材料の開発　　　　　　　　　　　　　　　
中西 康, 赤坂 司, 吉原 久美子, 中村 真理子, 吉田 靖弘, 日本歯科理工学会誌, 41, Special Issue79, 13, 13, May 2022
(一社)日本歯科理工学会, Japanese

破骨細胞様細胞への分化へ影響を与えるマイクロ・ナノパターンの探索　　　　　　　　　　　　　　　
赤坂 司, 横山 敦郎, 中西 康, 吉田 靖弘, 日本歯科理工学会誌, 41, Special Issue79, 31, 31, May 2022
(一社)日本歯科理工学会, Japanese

Dental innovation at Hokkaido University
吉田靖弘, 吉田靖弘, 中西康, 中西康, 赤坂司, 日下輝雄, 日下輝雄, 日下輝雄, 山本照子, 山本照子, 山本照子, 日本歯科医学会連合雑誌, 1, 2022

前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材-骨・歯周組織形成能の評価-
金本佑生実, 宮治裕史, 西田絵利香, 宮田さほり, 眞弓佳代子, 吉野友都, 加藤昭人, 菅谷勉, 赤坂司, 大矢根綾子, 日本歯周病学会会誌(Web), 64, 2022

破骨細胞の吸収窩形成へのマイクロ・ナノパターンの影響　　　　　　　　　　　　　　　
赤坂 司, 中西 康, 吉田 靖弘, 日本歯科理工学会誌, 40, Special Issue77, 31, 31, Mar. 2021
(一社)日本歯科理工学会, Japanese

北海道・東北地方会5大学の教育への取り組み—Current dental materials education in five dental schools in Hokkaido and Tohoku branch
遠藤 一彦, 鈴木 治, 石田 喜紀, 高橋 正敏, 澤田 智史, 武本 真治, 赤坂 司, 根聿 尚史, 日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices / 日本歯科理工学会 編, 40, 1, 85, 94, Jan. 2021
日本歯科理工学会, Japanese

S-PRGナノフィラーを用いた抗菌性歯面コーティングによる臨床パラメーターの改善-イヌ実験的歯周炎における検討-
眞弓佳代子, 宮治裕史, 宮田さほり, 西田絵利香, 降旗友和, 金本佑生実, 菅谷勉, 蔀佳奈子, 赤坂司, 日本歯周病学会会誌(Web), 63, 2021

リゾチーム-金ナノクラスター/ローズベンガル複合体の抗菌・抗バイオフィルム光線力学的活性
岡本一絵, 宮治裕史, 宮田さほり, 菅谷勉, 蔀佳奈子, 赤坂司, 齋田慧, 川崎英也, 日本歯周病学会会誌(Web), 63, 2021

マイクロ・ナノパターンが骨系細胞に与える影響について
沼本真一郎, 横山敦郎, 赤坂司, 吉田靖弘, 北海道外科雑誌, 66, 1, 2021

破骨細胞分化誘導へのマイクロ・ナノパターンの影響　　　　　　　　　　　　　　　
赤坂 司, 玉井 美保, 吉田 靖弘, 日本歯科理工学会誌, 39, Special Issue75, 8, 8, Apr. 2020
(一社)日本歯科理工学会, Japanese

マイクロブラシ状コラーゲンからなる人工歯根膜を持つインプラントの開発　　　　　　　　　　　　　　　
赤坂 司, 医科学応用研究財団研究報告, 37, 36, 41, Feb. 2020
(公財)鈴木謙三記念医科学応用研究財団, Japanese

Surface modification of tooth by nanoimprinting and cell response
赤坂司, 砥粒加工学会誌, 64, 12, 2020

Trial produce of micro/nano-patterns to screening in initial evaluation of cell functions for biomedical applications
赤坂司, 伊東駿也, 中川勝, 宮内昭浩, 生体医歯工学共同研究拠点成果報告会, 2019, 2020

マイクロブラシ状コラーゲンからなる人工歯根膜を持つインプラントの開発
赤坂司, 医科学応用研究財団研究報告(CD-ROM), 37, 2020

β-TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペプチド骨補填材による骨形成効果
降籏 友和, 宮治 裕史, 西田 絵利香, 加藤 昭人, 宮田 さほり, 蔀 佳奈子, 眞弓 佳代子, 金本 佑生実, 菅谷 勉, 赤坂 司, 日本歯周病学会会誌, 61, 秋季特別, 126, 126, Oct. 2019
(NPO)日本歯周病学会, Japanese

β-TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペプチド骨補填材による骨形成効果
降籏 友和, 宮治 裕史, 西田 絵利香, 加藤 昭人, 宮田 さほり, 蔀 佳奈子, 眞弓 佳代子, 金本 佑生実, 菅谷 勉, 赤坂 司, 日本歯周病学会会誌, 61, 秋季特別, 126, 126, Oct. 2019
(NPO)日本歯周病学会, Japanese

β‐TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペブチド骨補填材による骨形成効果
降籏友和, 宮治裕史, 西田絵利香, 加藤昭人, 宮田さほり, 蔀佳奈子, 眞弓佳代子, 金本佑生実, 菅谷勉, 赤坂司, 日本歯周病学会会誌(Web), 61, 126, 01 Oct. 2019
Japanese

マイクロ・ナノ構造への血液関連細胞の付着性　　　　　　　　　　　　　　　
赤坂 司, 玉井 美保, 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue74, 34, 34, Sep. 2019
(一社)日本歯科理工学会, Japanese

ナノポーラス物質含有による歯科用シーラント材料から薬剤徐放特性の検討　　　　　　　　　　　　　　　
江良 裕子, 中西 康, 阿部 薫明, 玉井 美保, 赤坂 司, 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue74, 47, 47, Sep. 2019
(一社)日本歯科理工学会, Japanese

Groove/Pillarパターン化チタン表面が細胞接着及び増殖に与える影響　　　　　　　　　　　　　　　
加我 公行, 赤坂 司, 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue73, 37, 37, Apr. 2019
(一社)日本歯科理工学会, Japanese

カゴ状ナノシリカ粒子を用いた薬剤徐放性歯科材料の開発　　　　　　　　　　　　　　　
江良 裕子, 阿部 薫明, 赤坂 司, 吉田 靖弘, 日本歯科理工学会誌, 38, Special Issue73, 43, 43, Apr. 2019
(一社)日本歯科理工学会, Japanese

マイクロ・ナノ構造が歯周組織関連細胞の挙動にもたらす影響　　　　　　　　　　　　　　　
高田 亮, 赤坂 司, 山方 秀一, 佐藤 嘉晃, 吉田 靖弘, 北海道歯学雑誌, 39, 2, 167, 167, Mar. 2019
北海道歯学会, Japanese

マイクロ・サブミクロパターン構造を付与したCOP film上における細胞挙動　　　　　　　　　　　　　　　
本間 淳, 赤坂 司, 山方 秀一, 佐藤 嘉晃, 吉田 靖弘, 北海道歯学雑誌, 39, 2, 167, 167, Mar. 2019
北海道歯学会, Japanese

マイクロ・ナノパターンに対する血液関連細胞の細胞接着
赤坂司, 砥粒加工学会学術講演会講演論文集(CD-ROM), 2019, 2019

マイクロ・ナノパターン表面構造における細胞応答
加我公行, 赤坂司, 吉田靖弘, 横山敦郎, 北海道外科雑誌, 64, 1, 2019

Bone forming ability of human type-I collagen-like recombinant peptide granules and submicron β-TCP
西田絵利香, 宮治裕史, 降籏友和, 加藤昭人, 宮田さほり, 蔀佳奈子, 眞弓佳代子, 金本佑生実, 菅谷勉, 赤坂司, 日本バイオマテリアル学会大会予稿集(Web), 41st, 2019

ナノβ‐TCP/ヒトI型コラーゲン様リコンビナントペプチド顆粒の骨形成特性評価
西田絵利香, 宮治裕史, 降籏友和, 宮田さほり, 加藤昭人, 金本佑生実, 菅谷勉, 赤坂司, 日本再生医療学会総会(Web), 18th, ROMBUNNO.P‐02‐099 (WEB ONLY), 2019
Japanese

マイクロ・ナノ構造が細胞接着にもたらす影響　　　　　　　　　　　　　　　
高田 亮, 赤坂 司, 山方 秀一, 佐藤 嘉晃, 飯田 順一郎, 吉田 靖弘, 日本矯正歯科学会大会プログラム・抄録集, 77回, 256, 256, Oct. 2018
(公社)日本矯正歯科学会, Japanese

多孔質シリカを用いた選択的薬剤徐放を実現する歯科用材料の開発　　　　　　　　　　　　　　　
江良 裕子, 中西 康, 阿部 薫明, 玉井 美保, 赤坂 司, 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 56, 56, Sep. 2018
(一社)日本歯科理工学会, Japanese

ユウロピウム錯体を用いた矯正歯科用蛍光ボンディング材の試作　　　　　　　　　　　　　　　
松本 愛子, 山方 秀一, 佐藤 嘉晃, 阿部 薫明, 赤坂 司, 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue72, 107, 107, Sep. 2018
(一社)日本歯科理工学会, Japanese

歯科材料に混合したナノポーラス物質からの薬剤徐放特性の追跡　　　　　　　　　　　　　　　
江良 裕子, 中西 康, 阿部 薫明, 赤坂 司, 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue71, 53, 53, Mar. 2018
(一社)日本歯科理工学会, Japanese

キャピラリーフォースによるマイクロピラー自己組織化による培養担体の調製　　　　　　　　　　　　　　　
赤坂 司, 玉井 美保, 阿部 薫明, 吉田 靖弘, 日本歯科理工学会誌, 37, Special Issue71, 72, 72, Mar. 2018
(一社)日本歯科理工学会, Japanese

薬剤徐放能を持つ多孔質シリカを用いた歯科用材料の開発
江良裕子, 坂東洋祐, 中西康, 阿部薫明, 玉井美保, 赤坂司, 飯田順一郎, 吉田靖弘, 化学系学協会北海道支部冬季研究発表会(Web), 2018, 2018

リゾチーム金ナノクラスター/ローズベンガル複合体の創製と光線力学的評価
岡本一絵, 宮治裕史, 宮田さほり, 蔀佳奈子, 川崎英也, 赤坂司, 菅谷勉, 日本バイオマテリアル学会大会予稿集(Web), 39回, 132, 132, Nov. 2017
日本バイオマテリアル学会, Japanese

ナノポーラス物質を用いたグラスアイオノマーセメントマトリックスからの薬剤モデル物質の徐放挙動　　　　　　　　　　　　　　　
江良 裕子, 中西 康, 阿部 薫明, 玉井 美保, 赤坂 司, 吉田 靖弘, 日本歯科理工学会誌, 36, 5, 356, 356, Sep. 2017
(一社)日本歯科理工学会, Japanese

硬化可能な歯科材料によるマイクロ・ナノパターニング　　　　　　　　　　　　　　　
赤坂 司, 加我 公行, 横山 敦郎, 阿部 薫明, 吉田 靖弘, 日本歯科理工学会誌, 36, 2, 82, 82, Mar. 2017
(一社)日本歯科理工学会, Japanese

Micro/nano-patterning of gelatin by crosslinking with genipin
赤坂司, 阿部薫明, 吉田靖弘, 日本歯科理工学会誌, 36, 5, 2017

ゲニピン添加マイクロ・ナノパターン化ゼラチン上での細胞接着
蒔田玲果, 赤坂司, 宮治裕史, 宮田さほり, 菅谷勉, 日本バイオマテリアル学会シンポジウム予稿集, 2016, 142, 142, 21 Nov. 2016
日本バイオマテリアル学会, Japanese

歯表面での3D微細構造構築によるバイオミメティック次世代歯科治療へ向けて
赤坂司, 加我公行, 宮治裕史, 横山敦郎, 吉田靖弘, 日本歯科医学会誌, 35, 36, 36, 31 Mar. 2016
日本歯科医学会, Japanese

Electroconductive treatment for SEM observation of wet-biological samples using room temperature ionic liquids-
江良裕子, 阿部薫明, 川上隼人, 河合功治, 赤坂司, 吉田靖弘, 日本歯科理工学会誌, 35, 2, 135, 135, 25 Mar. 2016
(一社)日本歯科理工学会, Japanese

Cell aggregation of periodontal ligament fibroblasts controlled by micro-patterns
赤坂司, 加我公行, 横山敦郎, 阿部薫明, 吉田靖弘, 日本歯科理工学会誌, 35, 2, 134, 134, 25 Mar. 2016
(一社)日本歯科理工学会, Japanese

A novel drug controlled release system using phoshorylated polysaccharides
阿部薫明, 沖原巧, 赤坂司, 吉田靖弘, 日本歯科理工学会誌, 35, 2, 128, 128, 25 Mar. 2016
(一社)日本歯科理工学会, Japanese

金クラスターとLED光照射器による光殺菌歯科治療法の開発
宮田さほり, 宮治裕史, 西田絵利香, 川崎英也, 山本真希, 滝田裕子, 牛島夏未, 岩永俊彦, 赤坂司, 田中佐織, 川浪雅光, 日本バイオマテリアル学会大会予稿集, 37th, 183, 183, 09 Nov. 2015
日本バイオマテリアル学会, Japanese

マイクロパターンにおける歯根膜線維芽細胞の細胞配向および移動
赤坂 司, 加我 公行, 横山 敦郎, 阿部 薫明, 吉田 靖弘, 日本歯科理工学会誌, 34, 5, 397, 397, Sep. 2015
(一社)日本歯科理工学会, Japanese

Behavior of cell adhesion on micro/nano-patterned materials
赤坂司, 加我公行, 阿部薫明, 吉田靖弘, 日本歯科理工学会誌, 34, 2, 58, 58, 25 Mar. 2015
(一社)日本歯科理工学会, Japanese

Biocompatibility and Toxicity of Nanobiomaterials 2014
Xiaoming Li, Sang Cheon Lee, Shuming Zhang, Tsukasa Akasaka, JOURNAL OF NANOMATERIALS, 2015, 2015
English, Others

Effects of cell type on cell adhesion of micro/nano-patterned composite resin
赤坂司, 加我公行, 阿部薫明, 吉田靖弘, 日本歯科理工学会誌, 33, 5, 466, 466, 20 Sep. 2014
(一社)日本歯科理工学会, Japanese

Development of protein stock solution using ionic liquids: A long-term stability of proteins at room temperature
阿部薫明, 金子恒太郎, 河合功治, 赤坂司, 吉田靖弘, 日本歯科理工学会誌, 33, 5, 446, 446, 20 Sep. 2014
(一社)日本歯科理工学会, Japanese

Fabrication of micro/nano-patterned gelatin for cell adhesion studies
加我公行, 赤坂司, 横山敦郎, 吉田靖弘, 日本歯科理工学会誌, 33, 5, 464, 464, 20 Sep. 2014
(一社)日本歯科理工学会, Japanese

【続・バイオマテリアルの境界科学】固体高分子表面の微細構造による細胞応答
赤坂 司, バイオマテリアル-生体材料-, 32, 3, 163, 173, Jul. 2014
日本バイオマテリアル学会, Japanese

イオン液体を用いたバイオマテリアルのSEM新規観察法の開発
兵野 篤, 米澤 徹, 阿部 薫明, 赤坂 司, 亘理 文夫, 北海道外科雑誌, 59, 1, 91, 92, Jun. 2014
北海道外科学会, Japanese

マイクロ・ナノパターンを付与したコンポジットレジン上への細胞接着
今村琢也, 宮治裕史, 赤坂司, 西田絵利香, 長尾敬志, 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 57th, 春季特別, 120, 120, 08 Apr. 2014
(NPO)日本歯周病学会, Japanese

マイクロ・ナノパターン化コンポジットレジンへの細胞接着
赤坂 司, 亘理 文夫, 日本歯科理工学会誌, 33, 2, 120, 120, Mar. 2014
(一社)日本歯科理工学会, Japanese

セラミックナノ粒子の細胞刺激性の検討
阿部 薫明, 岩寺 信喜, 半場 悠介, 山方 秀一, 赤坂 司, 八若 保孝, 飯田 順一郎, 亘理 文夫, 古月 文志, 日本歯科理工学会誌, 33, 2, 162, 162, Mar. 2014
(一社)日本歯科理工学会, Japanese

Biocompatibility and Toxicity of Nanobiomaterials 2013
Xiaoming Li, Sang Cheon Lee, Shuming Zhang, Tsukasa Akasaka, JOURNAL OF NANOMATERIALS, 2014, 2014
English, Others

Polymeric Scaffolds for Tissue Engineering
Xiaoming Li, Tsukasa Akasaka, Nicholas Dunne, INTERNATIONAL JOURNAL OF POLYMER SCIENCE, 2014, 2014
English, Others

キトサンを用いたマイクロパターン化フィルムの作製
西田絵利香, 宮治裕史, 赤坂司, 川浪雅光, 日本バイオマテリアル学会大会予稿集, 35th, 459, 459, 25 Nov. 2013
日本バイオマテリアル学会, Japanese

ユウロピウム錯体を用いた歯科矯正用ボンディング材への蛍光特性の付与　　　　　　　　　　　　　　　
半場 悠介, 山方 秀一, 中西 康, 赤坂 司, 飯田 順一郎, 亘理 文夫, 日本矯正歯科学会大会プログラム・抄録集, 72回, 215, 215, Oct. 2013
(公社)日本矯正歯科学会, Japanese

マイクロ・ナノパターン化したアパタイト担体への細胞接着
赤坂 司, 亘理 文夫, 日本歯科理工学会誌, 32, 5, 344, 344, Sep. 2013
(一社)日本歯科理工学会, Japanese

歯科応用に向けたポリ乳酸/層状ケイ酸塩ナノコンポジットの試作
中西 康, 山方 秀一, 半場 悠介, 赤坂 司, 亘理 文夫, 飯田 順一郎, 日本歯科理工学会誌, 32, 5, 372, 372, Sep. 2013
(一社)日本歯科理工学会, Japanese

骨芽細胞へと曝露したマイクロ/ナノマテリアルの生体刺激性の検討
阿部 薫明, 岩寺 信喜, 半場 悠介, 山方 秀一, 赤坂 司, 八若 保孝, 飯田 順一郎, 亘理 文夫, 日本歯科理工学会誌, 32, 5, 373, 373, Sep. 2013
(一社)日本歯科理工学会, Japanese

ヒト上皮3次元培養組織モデルを用いた生体材料‐軟組織間の封鎖性評価
古橋一憲, 北川善政, 赤坂司, 亘理文夫, 青沼仁志, 北海道外科雑誌, 58, 1, 87, 88, 20 Jun. 2013
北海道外科学会, Japanese

ナノ微粒子に対する細胞応答 タイムラプス法による動的挙動連続観察
岩寺 信喜, 伊藤 佐智子, 八若 保孝, 赤坂 司, 亘理 文夫, 北海道外科雑誌, 58, 1, 89, 89, Jun. 2013
北海道外科学会, Japanese

歯科矯正用ボンディング材の視認性向上に向けた試み
半場 悠介, 山方 秀一, 赤坂 司, 宇尾 基弘, 飯田 順一郎, 亘理 文夫, 日本歯科理工学会誌, 32, 2, 111, 111, Mar. 2013
(一社)日本歯科理工学会, Japanese

最新の歯学 マイクロ・ナノパターン化バイオマテリアルの歯科領域での応用
赤坂 司, 亘理 文夫, 北海道歯学雑誌, 33, 2, 189, 191, Mar. 2013
北海道歯学会, Japanese

Preparation of patterned biomaterials with micro- and nano-topographies
赤坂司, 阿部薫明, 亘理文夫, 日本歯科理工学会誌, 32, 2, 2013

チタン製の人工骨・人工歯根が骨に結合する生化学的メカニズム
久保木芳徳, 古沢利武, 佐藤正明, 孫永こん, 鵜沼英郎, 滝田裕子, 藤澤隆一, 阿部薫明, 赤坂司, 亘理文夫, SAMMONS Rachel L., 北海道外科雑誌, 58, 1, 2013

SEM observation of biological samples using room temperature ionic liquids
ABE SHIGEAKI, HYONO ATSUSHI, AKASAKA TSUKASA, WATARI FUMIO, YONEZAWA TETSU, 日本歯科理工学会誌, 31, 5, 442, 442, 25 Sep. 2012
Ionic liquid is a kind of salt as a liquid even at room temperature. Because of their unique properties such as fluidity, high ionic conductivity and negligible vapor pressure, they have been attention for widely applications even under vacuum conditions. In this study, we investigated the ionic liquid for SEM pretreatment of several biomaterials. With regard to IL coating on the sample surface, we succeeded in high-quality observation of biomaterials. In addition, we also succeeded in applying the IL preparation to SEM-EDS measurement. These results suggested that this simple and rapid conductivity preparation for SEM observation., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese

P-9 Evaluation of adhesion between biomaterial and epithelium using human 3D epidermal model
Furuhashi K, Akasaka T, Kitagawa Y, Watari F, The journal of the Japanese Society for Dental Materials and Devices, 31, 5, 392, 392, 25 Sep. 2012
The development of nanotechnology has created various nano-scaled biomaterials. Micro/nanosizing of biomaterials can enhance function ability compared to macro size materials. In this study, nano-scaled hydroxyapatite (SHAp) composite polymer and 3D epidermal tissue model were used for cell culture experiment to evaluate the closure between biomaterial and epithelial. Using fibroblast and epidermal keratinocyte progenitor cells, cellular adhesiveness was examined. SHAp was observed to adhere to 3D epidermal model through the keratinized layer., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese

Anti-bacterial activity of chitosan film with nano-structure
AKASAKA TSUKASA, ABE SHIGEAKI, WATARI FUMIO, 日本歯科理工学会誌, 31, 5, 367, 367, 25 Sep. 2012
(一社)日本歯科理工学会, Japanese

蛍光特性を有する歯科矯正用ボンディング材の特性評価
半場 悠介, 山方 秀一, 赤坂 司, 宇尾 基弘, 飯田 順一郎, 亘理 文夫, 日本歯科理工学会誌, 31, 5, 368, 368, Sep. 2012
(一社)日本歯科理工学会, Japanese

カーボンナノチューブ薄膜上での細胞培養
赤坂司, 阿部薫明, 亘理文夫, 橋本剛, 北海道外科雑誌, 57, 1, 88, 88, 20 Jun. 2012
北海道外科学会, Japanese

A-23 Cellular behavior on nano-scaled hydroxyapatite composite materials
Furuhashi K, Akasaka T, Kitagawa Y, Watari F, The journal of the Japanese Society for Dental Materials and Devices, 31, 2, 136, 136, 25 Mar. 2012
The development of nanotechnology has created various nano-scaled biomaterials. Micro/nanosizing of biomaterials can enhance function ability compared to macro size materials. In this study, nano-scaled hydroxyapatite (SHAp) composite polymer and 3D epidermal tissue model were used for cell culture experiment to evaluate the cellular adhesiveness. Using fibroblast and epidermal keratinocyte progenitor cells examined cellular behavior (mainly cellular adhesiveness). SEM observation showed that more cells were attached and extended larger. Many fines filopodias were developed from the cells. These suggest a strong cell adhesive capacity of SHAp., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese

Cell culture with carbon nanotube films and cell manipulation
AKASAKA TSUKASA, ABE SHIGEAKI, WATARI FUMIO, 日本歯科理工学会誌, 31, 2, 91, 91, 25 Mar. 2012
(一社)日本歯科理工学会, Japanese

カーボンナノチューブのバイオ応用
赤坂司, 北海道歯学雑誌, 32, 2, 235, 236, 15 Mar. 2012
北海道歯学会, Japanese

歯科矯正用ボンディング材への蛍光性物質の添加効果
半場 悠介, 山方 秀一, 赤坂 司, 宇尾 基弘, 飯田 順一郎, 亘理 文夫, 日本歯科理工学会誌, 31, 2, 85, 85, Mar. 2012
(一社)日本歯科理工学会, Japanese

マイクロ/ナノ粒子曝露下の骨芽細胞様細胞と肝細胞の影響
岩寺 信喜, 阿部 薫明, 石川 紘佑, 赤坂 司, 宇尾 基弘, 八若 保孝, 亘理 文夫, 日本歯科理工学会誌, 31, 2, 97, 97, Mar. 2012
(一社)日本歯科理工学会, Japanese

タンパク質とチタンの反応 インプラント定着の機構解明を目指して　　　　　　　　　　　　　　　
久保木 芳徳, 古沢 利武, 佐藤 正明, 孫 永こん, 鵜沼 英郎, 滝田 裕子, 藤澤 隆一, 阿部 薫明, 赤坂 司, 亘理 文夫, 北海道歯学雑誌, 32, 2, 250, 251, Mar. 2012
北海道歯学会, Japanese

放電プラズマ焼結した歯科用ジルコニアセラミックスの微細欠陥と寸法精度評価
宇尾基弘, 亘理文夫, 赤坂司, 阿部薫明, 後藤孝, 木村久道, 大森守, 大久保昭, 東北大学金属材料研究所金属ガラス総合研究センター共同利用研究報告書, 2011, 2012

Interaction between carbon nanotubes and biomolecules: An in vitro and in silico study
阿部薫明, 小峰梨果, 石川紘佑, 赤坂司, 亘理文夫, 田地川浩人, 日本歯科理工学会誌, 31, 2, 2012

Preference of adsorbed protein on carbon nanotubes
森川真衣, 久保木芳徳, 赤坂司, 阿部薫明, 亘理文夫, 日本歯科理工学会誌, 31, 2, 2012

Biocompatibility and Toxicity of Nanobiomaterials
Xiaoming Li, Sang Cheon Lee, Shuming Zhang, Tsukasa Akasaka, JOURNAL OF NANOMATERIALS, 2012, 2012
English, Others

多層カーボンナノチューブの骨髄間質細胞への影響　　　　　　　　　　　　　　　
平田 恵理, 赤坂 司, 滝田 裕子, 宇尾 基弘, 亘理 文夫, 横山 敦郎, 日本バイオマテリアル学会大会予稿集, 33回, 383, 383, Nov. 2011
日本バイオマテリアル学会, Japanese

P-46 Development of a novel SEM observation using an ionic liquid
Abe S, Hyono A, Akasaka T, Watari F, Yonezawa T, The journal of the Japanese Society for Dental Materials and Devices, 30, 5, 349, 349, 25 Sep. 2011
Ionic liquid is a kind of salt as a liquid even at room temperature. Because of their unique properties such as negligible vapor pressure at room temperature and high ionic conductivity, they have been attention for widely applications even under vacuum conditions. In this study, we investigated the ionic liquid for treatment of insulating SEM samples. Ionic liquid was found to put electrical conductivity to the samples similar to a metal or carbon coating., The Japanese Society for Dental Materials and Devices (JSDMD), Japanese

ポリ乳酸の分解生成物の解析
佐々木 朗子, 阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 日本歯科理工学会誌, 30, 5, 317, 317, Sep. 2011
(一社)日本歯科理工学会, Japanese

マイクロ・ナノ無機粒子の各種細胞への影響
岩寺 信喜, 阿部 薫明, 石川 紘佑, 赤坂 司, 宇尾 基弘, 八若 保孝, 亘理 文夫, 日本歯科理工学会誌, 30, 5, 318, 318, Sep. 2011
(一社)日本歯科理工学会, Japanese

カーボンナノチューブ培養担体への誘電泳動を用いた細胞集積
松岡真琴, 赤坂司, 橋本剛, 亘理文夫, 戸塚靖則, ナノ学会大会講演予稿集, 9th, 297, 02 Jun. 2011
Japanese

カーボンナノチューブ薄膜上での細胞培養
赤坂司, 松岡真琴, 横山敦郎, 橋本剛, 亘理文夫, ナノ学会大会講演予稿集, 9th, 293, 02 Jun. 2011
Japanese

生体を模倣した表面化学修飾による石灰化反応の制御　　　　　　　　　　　　　　　
阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 藤井 悠平, 楠原 有紗, 大和谷 恵実, 石田 智毅, 林 大輔, 高田 知哉, 北海道外科雑誌, 56, 1, 74, 75, Jun. 2011
北海道外科学会, Japanese

Carbon Nanotube-coated Silicone as a Flexible Biomedical Material
Nano Biomed, 3, Special Issue, 119, Jun. 2011
Japanese, Others

Cell proliferation on Carbon Nanotubes Coated Dishes in Different Cell Lines
Nano Biomed, 3, Special Issue, 78, Jun. 2011
Japanese, Others

Cell Culture on Carbon Nanotubes Thin Films
Nano Biomed, 3, Special Issue, 181, Jun. 2011
Japanese

放電プラズマ焼結による歯科用ジルコニアセラミックスの作製と機械的特性評価
宇尾基弘, 亘理文夫, 赤坂司, 阿部薫明, 後藤孝, 木村久道, 大森守, 大久保昭, 東北大学金属材料研究所金属ガラス総合研究センター共同利用研究報告書, 2010, 2011

歯科インプラント用カーボンナノチューブコーティング陽極酸化チタンの開発と観察
井上沙織, 宇尾基弘, 坂入正敏, 平田恵理, LEE Min-Ho, BAE Tae-Sung, 赤坂司, 亘理文夫, 横山敦郎, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2011, 2011

ラジカル反応によるカルボキシル化多層カーボンナノチューブの作製と透明導電体作製への応用
中山勝利, 阿部薫明, 林大輔, 赤坂司, 宇尾基弘, 亘理文夫, 高田知哉, 化学系学協会北海道支部冬季研究発表会講演要旨集, 2011, 2011

ナノ物質の体内循環挙動の追跡とその生体親和性
阿部薫明, 岩寺信喜, 伊藤佐智子, 赤坂司, 宇尾基弘, 八若保孝, 高田知哉, 米澤徹, 亘理文夫, ナノ学会大会講演予稿集, 9th, 2011

チタン反応性タンパク質をクロマトグラフィーと質量分析によって血清中より検出した
久保木芳徳, 古沢利武, 佐藤正明, SUN Yongkun, 鵜沼英郎, 阿部薫明, 赤坂司, 亘理文夫, 滝田裕子, 安孫子宜光, 日本バイオマテリアル学会大会予稿集, 33rd, 2011

タンパク質とチタンの反応:インプラント定着の機構解明を目指して
久保木芳徳, 古沢利武, 佐藤正明, 孫永こん, 鵜沼英郎, 滝田裕子, 藤澤隆一, 阿部薫明, 赤坂司, 亘理文夫, 北海道歯学会学術大会プログラム講演内容抄録, 2011, 2011

カーボンナノチューブのバイオ応用へ向けて
赤坂司, 北海道歯学雑誌, 31, 2, 129, 129, 15 Dec. 2010
北海道歯学会, Japanese

カーボンナノチューブ薄膜上における細胞培養
赤坂司, 橋本剛, 松岡真琴, 横山敦郎, 亘理文夫, 日本バイオマテリアル学会大会予稿集, 32nd, 142, 142, 29 Nov. 2010
日本バイオマテリアル学会, Japanese

誘電泳動を利用したカーボンナノチューブ培養担体への細胞誘導
松岡真琴, 赤坂司, 戸塚靖則, 亘理文夫, 日本バイオマテリアル学会大会予稿集, 32nd, 200, 200, 29 Nov. 2010
日本バイオマテリアル学会, Japanese

マイクロ・ナノ粒子の体内循環挙動の追跡と生体への影響　　　　　　　　　　　　　　　
岩寺 信喜, 石川 紘佑, 伊藤 佐智子, 阿部 薫明, 赤坂 司, 宇尾 基弘, 八若 保孝, 亘理 文夫, 高田 知哉, 米澤 徹, 日本バイオマテリアル学会大会予稿集, 32回, 323, 323, Nov. 2010
日本バイオマテリアル学会, Japanese

Cell behavior in different cell types on thin films of carbon nanotubes
AKASAKA TSUKASA, ABE SHIGEAKI, UO MOTOHIRO, WATARI FUMIO, 日本歯科理工学会誌, 29, 5, 460, 460, 20 Sep. 2010
(一社)日本歯科理工学会, Japanese

S-PRGフィラー溶出液に浸漬したヒト歯質中ストロンチウムのXAFSによる構造解析
宇尾 基弘, 阿部 薫明, 赤坂 司, 亘理 文夫, 中塚 稔之, 日本歯科理工学会誌, 29, 5, 383, 383, Sep. 2010
(一社)日本歯科理工学会, Japanese

亜鉛錯体化合物による石灰化反応の制御
藤井 悠平, 楠原 有紗, 大和谷 恵実, 石田 智毅, 阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 林 大輔, 高田 知哉, 日本歯科理工学会誌, 29, 5, 438, 438, Sep. 2010
(一社)日本歯科理工学会, Japanese

有機マイクロ/ナノ粒子の体内循環挙動の追跡　　　　　　　　　　　　　　　
阿部 薫明, 伊藤 佐智子, 大口 郁子, 赤坂 司, 宇尾 基弘, 亘理 文夫, 林 大輔, 高田 知哉, 北海道外科雑誌, 55, 1, 84, 84, Jun. 2010
北海道外科学会, Japanese

マウスに投与したマイクロ・ナノ無機粒子の生体内分布の追跡と細胞への影響　　　　　　　　　　　　　　　
岩寺 信喜, 阿部 薫明, 石川 紘佑, 赤坂 司, 宇尾 基弘, 大口 郁子, 八若 保孝, 亘理 文夫, 米澤 徹, 北海道外科雑誌, 55, 1, 84, 85, Jun. 2010
北海道外科学会, Japanese

カーボンナノチューブ上の骨芽細胞、骨髄細胞の培養　　　　　　　　　　　　　　　
北原 浩美, 井上 農夫男, 久保木 芳徳, 赤坂 司, 宇尾 基弘, 阿部 薫明, 亘理 文夫, 北海道外科雑誌, 55, 1, 85, 85, Jun. 2010
北海道外科学会, Japanese

Influence of carbon nanotubes on biofilm growth
AKASAKA TSUKASA, ABE SHIGEAKI, UO MOTOHIRO, WATARI FUMIO, 日本歯科理工学会誌, 29, 2, 210, 210, 25 Mar. 2010
(一社)日本歯科理工学会, Japanese

Cell adhesion to the materials treated with carbon nanotubes
MATSUOKA MAKOTO, AKASAKA TSUKASA, TOTSUKA YASUNORI, WATARI FUMIO, 日本歯科理工学会誌, 29, 2, 120, 120, 25 Mar. 2010
(一社)日本歯科理工学会, Japanese

微小部蛍光XAFS測定による肺胞洗浄液からの超硬合金成分の検出
宇尾 基弘, 阿部 薫明, 赤坂 司, 亘理 文夫, 日本歯科理工学会誌, 29, 2, 150, 150, Mar. 2010
(一社)日本歯科理工学会, Japanese

マウス体内でのマイクロ・ナノ物質の体内動態の追跡
岩寺 信喜, 伊藤 佐智子, 阿部 薫明, 大口 郁子, 赤坂 司, 宇尾 基弘, 八若 保孝, 亘理 文夫, 日本歯科理工学会誌, 29, 2, 204, 204, Mar. 2010
(一社)日本歯科理工学会, Japanese

カーボンナノチューブスカフォールド上における骨芽細胞、骨髄細胞の培養
北原 浩美, 久保木 芳徳, 赤坂 司, 宇尾 基弘, 阿部 薫明, 亘理 文夫, 井上 農夫男, 日本歯科理工学会誌, 29, 2, 207, 207, Mar. 2010
(一社)日本歯科理工学会, Japanese

カーボンナノ材料のSPS焼結体による生体材料の開発
宇尾基弘, 亘理文夫, 赤坂司, 阿部薫明, 木村久道, 大森守, 大久保昭, 東北大学金属材料研究所金属ガラス総合研究センター共同利用研究報告書, 2009, 2010

酵素モデル化合物の合成と石灰化反応制御への応用
阿部薫明, 藤井悠平, 楠原有紗, 大和谷恵実, 石田智毅, 赤坂司, 宇尾基弘, 亘理文夫, 林大輔, 高田知哉, 無機リン化学討論会講演要旨集, 20th, 2010

細菌・細胞へのナノチューブの作用と応用
亘理文夫, 赤坂司, 横山敦郎, 宇尾基弘, ポリマー材料フォーラム講演予稿集, 19th, 2010

酵素モデル化合物による石灰化反応の制御
阿部薫明, 藤井悠平, 楠原有紗, 大和谷恵実, 石田智毅, 赤坂司, 宇尾基弘, 亘理文夫, 林大輔, 高田知哉, 傾斜機能材料論文集, 24(CD-ROM), 2010

Morphology-controlled calcification (CaCO3): A donut-shape crystal growth breaking through a carbon nanotube “blanket”　　　　　　　　　　　　　　　
Shigeaki Abe, Tomoki Ishida, Chika Koyama, Tsukasa Akasaka, Motohiro Uo, Fumio Watari, Journal of the Australian Ceramic Society, 46, 48, 2010

ナノ粒子の生体反応誘発性 Biointeractive、Bioreactive特性について　　　　　　　　　　　　　　　
亘理 文夫, 横山 敦郎, 赤坂 司, 宇尾 基弘, 阿部 薫明, 伊藤 佐智子, 大口 郁子, 八若 保孝, 日本バイオマテリアル学会大会予稿集, 31回, 195, 195, Nov. 2009
日本バイオマテリアル学会, Japanese

各種マイクロ・ナノ粒子の生体内での分布状態の追跡とその可視化　　　　　　　　　　　　　　　
阿部 薫明, 岩寺 信喜, 武藤 麻未, 林 大輔, 大口 郁子, 赤坂 司, 宇尾 基弘, 亘理 文夫, 高田 知哉, 米澤 徹, 日本バイオマテリアル学会大会予稿集, 31回, 414, 414, Nov. 2009
日本バイオマテリアル学会, Japanese

Capture of bacteria and bactericidal effect using carbon nanotubes
AKASAKA TSUKASA, ABE SHIGEAKI, UO MOTOHIRO, WATARI FUMIO, 歯科材料・器械, 28, 5, 363, 363, 10 Sep. 2009
(一社)日本歯科理工学会, Japanese

酵素モデル化合物の作成と石灰化反応制御への応用
大和谷 恵実, 石田 智毅, 阿部 薫明, 大口 郁子, 赤坂 司, 宇尾 基弘, 亘理 文夫, 林 大輔, 高田 知哉, 歯科材料・器械, 28, 5, 262, 262, Sep. 2009
(一社)日本歯科理工学会, Japanese

石灰化溶液によるカーボンナノチューブ上でのアパタイト様析出物の観察
越川 高光, 久保木 芳徳, 阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 歯科材料・器械, 28, 5, 268, 268, Sep. 2009
(一社)日本歯科理工学会, Japanese

マウス由来のES細胞とiPS細胞の細胞分化に対するカーボンナノチューブの影響
今井 弘一, 武田 昭二, 阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 歯科材料・器械, 28, 5, 309, 309, Sep. 2009
(一社)日本歯科理工学会, Japanese

無機ケイ酸塩ナノチューブ(イモゴライト)を用いた細胞培養担体の開発
石川 紘佑, 赤坂 司, 阿部 薫明, 宇尾 基弘, 八若 保孝, 亘理 文夫, 歯科材料・器械, 28, 5, 352, 352, Sep. 2009
(一社)日本歯科理工学会, Japanese

カーボンナノチューブ(CNT)に対する肝細胞の挙動
伊藤 佐智子, 赤坂 司, 八若 保孝, 亘理 文夫, 歯科材料・器械, 28, 5, 355, 355, Sep. 2009
(一社)日本歯科理工学会, Japanese

カーボンナノチューブスカフォールド上における骨芽細胞、骨髄細胞の培養
北原 浩美, 久保木 芳徳, 赤坂 司, 亘理 文夫, 井上 農夫男, 歯科材料・器械, 28, 5, 356, 356, Sep. 2009
(一社)日本歯科理工学会, Japanese

FRP矯正ワイヤー開発に向けた熱可塑性樹脂のナノコンポジット化
山方 秀一, 赤坂 司, 宇尾 基弘, 飯田 順一郎, 亘理 文夫, 歯科材料・器械, 28, 5, 384, 384, Sep. 2009
(一社)日本歯科理工学会, Japanese

人工酵素モデルを用いた石灰化反応の制御　　　　　　　　　　　　　　　
林 大輔, 高田 知哉, 阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 石田 智毅, 大和谷 恵美, 北海道外科雑誌, 54, 1, 75, 75, Jun. 2009
北海道外科学会, Japanese

肝臓細胞の増殖におよぼすカーボンナノチューブの影響について　　　　　　　　　　　　　　　
伊藤 佐智子, 赤坂 司, 八若 保孝, 亘理 文夫, 北海道外科雑誌, 54, 1, 76, 76, Jun. 2009
北海道外科学会, Japanese

骨芽細胞様細胞の成長に及ぼすImogoliteおよびCNTsスキャホールドの影響
石川 紘佑, 赤坂 司, 鈴木 正哉, 亘理 文夫, 八若 保孝, 小児歯科学雑誌, 47, 2, 347, 347, Apr. 2009
(公社)日本小児歯科学会, Japanese

正常肝細胞と癌肝細胞に対するカーボンナノチューブ(CNT)の影響について
伊藤 佐智子, 赤坂 司, 亘理 文夫, 八若 保孝, 小児歯科学雑誌, 47, 2, 353, 353, Apr. 2009
(公社)日本小児歯科学会, Japanese

柔軟性を持ったカーボンナノチューブによる細菌の捕捉
赤坂司, 阿部薫明, 宇尾基弘, 亘理文夫, 歯科材料・器械, 28, 2, 83, 83, 25 Mar. 2009
(一社)日本歯科理工学会, Japanese

カーボンナノチューブ上の細胞培養
亘理文夫, 赤坂司, 寺田典子, 越川高光, 宇尾基弘, 北川善政, 表面技術協会講演大会講演要旨集, 119th, 217, 02 Mar. 2009
Japanese

Ni-Ti合金及びステンレス鋼の生体内微量溶出物のXAFSによる状態分析
宇尾 基弘, 阿部 薫明, 赤坂 司, 亘理 文夫, 歯科材料・器械, 28, 2, 39, 39, Mar. 2009
(一社)日本歯科理工学会, Japanese

肝臓細胞の増殖に及ぼすCNTの影響
伊藤 佐智子, 赤坂 司, 八若 保孝, 亘理 文夫, 歯科材料・器械, 28, 2, 46, 46, Mar. 2009
(一社)日本歯科理工学会, Japanese

大気中でのオゾン-水クラスターについての理論的研究
阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 田地川 浩人, 歯科材料・器械, 28, 2, 82, 82, Mar. 2009
(一社)日本歯科理工学会, Japanese

PBS中におけるカーボンナノチューブ表面でのアパタイト様化合物の形成
越川 高光, 宇尾 基弘, 赤坂 司, 久保木 芳徳, 亘理 文夫, 歯科材料・器械, 28, 2, 113, 113, Mar. 2009
(一社)日本歯科理工学会, Japanese

亜鉛-イミダゾール錯体を持つ酵素モデル化合物による石灰化反応の制御
阿部薫明, 林大輔, 大和谷恵実, 石田智毅, 赤坂司, 宇尾基弘, 亘理文夫, 高田知哉, 錯体化学討論会講演要旨集, 59th, 2009

PBS中におけるカーボンナノチューブ表面でのアパタイトの形成
越川高光, 宇尾基弘, 赤坂司, 久保木芳徳, 亘理文夫, 化学系学協会北海道支部冬季研究発表会講演要旨集, 2009, 2009

ナノ微粒子の体内動態可視化法の開発 生体に投与した有機・無機粒子の体内動態のイメージングに関する研究
亘理文夫, 阿部薫明, 久保木芳徳, 森田学, 八若保孝, 宇尾基弘, 赤坂司, 江崎光恵, 岩寺信喜, ナノ微粒子の体内動態可視化法の開発 平成18-20年度 総合研究報告書, 2009

ナノ微粒子の体内動態可視化法の開発 生体に投与した有機・無機粒子の体内動態のイメージングに関する研究
亘理文夫, 阿部薫明, 久保木芳徳, 八若保孝, 宇尾基弘, 赤坂司, 岩寺信喜, ナノ微粒子の体内動態可視化法の開発 平成20年度 総括・分担研究報告書, 2009

ナノ微粒子の体内動態可視化法の開発 カーボンナノチューブが細胞に及ぼす影響に関する研究
戸塚靖則, 高師則行, 赤坂司, 松岡真琴, 亘理文夫, ナノ微粒子の体内動態可視化法の開発 平成20年度 総括・分担研究報告書, 2009

ナノ微粒子の体内動態可視化法の開発 細胞培養におけるカーボンナノチューブ膜の影響
亘理文夫, 宇尾基弘, 赤坂司, 阿部勲明, ナノ微粒子の体内動態可視化法の開発 平成20年度 総括・分担研究報告書, 2009

Application of flake shaped glass filler containing polycarbonate for non-clasp partial denture
佐々木朗子, 宇尾基弘, 岩崎佳治, 阿部薫明, 赤坂司, 亘理文夫, 歯科材料・器械, 28, 5, 2009

ナノ微粒子の体内動態可視化法の開発 ナノチューブ,ナノ微粒子,マイクロ微粒子の組織反応性とバイオ応用に関する研究
戸塚靖則, 高師則行, 赤坂司, 松岡真琴, 亘理文夫, ナノ微粒子の体内動態可視化法の開発 平成18-20年度 総合研究報告書, 2009

ナノ微粒子の体内動態可視化法の開発 フラーレンマーカーの合成およびカーボンナノチューブのバイオ応用
亘理文夫, 宇尾基弘, 赤坂司, 阿部勲明, ナノ微粒子の体内動態可視化法の開発 平成18-20年度 総合研究報告書, 2009

Culturing osteoblast-like cells on a carbon nanotube-coated dish. Nano Biomedicine
MATSUOKA Makoto, AKASAKA Tsukasa, HASHIMOTO Takeshi, TOTSUKA Yasunori, WATARI Fumio, Nano Biomedicine, 1, 1, 17, 25, 2009

Controlled calcification using polyanionic macromolecules with an active reaction center analogue of carbonic anhydrase
Shigeaki Abe, Tomoki Ishida, Emi Yamatoya, Daisuke Hayashi, Tsukasa Akasaka, Motohiro Uo, Fumio Watari, Tomoya Takada, Nano Biomedicine, 1, 1, 51, 58, 2009

Internal distribution of several inorganic microparticles in mice
Shigeaki Abe, Chika Koyama, Tsukasa Akasaka, Motohiro Uo, Yoshinori Kuboki, Fumio Watari, BIOCERAMICS 21, 396-398, 539, 542, 2009
English

単層カーボンナノチューブ透明導電性薄膜上での細胞培養
赤坂司, 阿部薫明, 宇尾基弘, 亘理文夫, 歯科材料・器械, 27, 5, 392, 392, 05 Sep. 2008
(一社)日本歯科理工学会, Japanese

高い細胞接着性と電気伝導性を併せ持つ生体再建材料の開発
松岡真琴, 赤坂司, 戸塚靖則, 亘理文夫, 歯科材料・器械, 27, 5, 358, 358, 05 Sep. 2008
(一社)日本歯科理工学会, Japanese

ガラスフレークフィラーを用いた高流動性歯科用コンポジットレジンの開発
宇尾 基弘, 阿部 薫明, 赤坂 司, 亘理 文夫, 歯科材料・器械, 27, 5, 331, 331, Sep. 2008
(一社)日本歯科理工学会, Japanese

カーボンナノチューブスキャフォルドの性能に対するナノ構造の効果(Effect of Nanostructure on the Performance of Carbon Nanotubes Scaffolds)
リ・シャオミン, 宇尾 基弘, 阿部 薫明, 赤坂 司, 亘理 文夫, 歯科材料・器械, 27, 5, 427, 427, Sep. 2008
(一社)日本歯科理工学会, English

ケイ酸塩ナノチューブ(イモゴライト)上での骨芽細胞培養
石川 紘佑, 赤坂 司, 八若 保孝, 亘理 文夫, 歯科材料・器械, 27, 5, 433, 433, Sep. 2008
(一社)日本歯科理工学会, Japanese

バイオミメティクポリマーによる石灰化反応の制御
石田 智毅, 阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 歯科材料・器械, 27, 5, 438, 438, Sep. 2008
(一社)日本歯科理工学会, Japanese

単層カーボンナノチューブ透明導電性薄膜での骨芽細胞様細胞の培養
赤坂司, 横山敦郎, 松岡真琴, 阿部薫明, 宇尾基弘, 亘理文夫, 橋本剛, 北海道外科雑誌, 53, 1, 122, 122, 20 Jun. 2008
北海道外科学会, Japanese

マウス体内での有機・無機粒子の体内循環挙動の追跡
阿部 薫明, 江崎 光恵, 赤坂 司, 宇尾 基弘, 森田 学, 亘理 文夫, 小山 千佳, Rosca Iosif Danel, 久保木 芳徳, 北海道外科雑誌, 53, 1, 122, 122, Jun. 2008
北海道外科学会, Japanese

ヤギシャンク骨欠損モデルを用いたキチン質繊維強化とナノアパタイト/コラーゲンコンポジットの骨修復特性評価
Li Xiaoming, Feng Qingling, Cui Fuzhai, 宇尾 基弘, 赤坂 司, 亘理 文夫, 北海道外科雑誌, 53, 1, 122, 123, Jun. 2008
北海道外科学会, Japanese

ナノマテリアルの評価手法に関する研究の進展 組織反応と体内動態の可視化研究について　　　　　　　　　　　　　　　
亘理 文夫, 阿部 薫明, 宇尾 基弘, 江崎 光恵, 森田 学, 平田 恵理, 横山 敦郎, 赤坂 司, 韮澤 崇, 佐藤 義倫, 田路 和幸, The Journal of Toxicological Sciences, 33, Suppl., S37, S37, Jun. 2008
(一社)日本毒性学会, Japanese

単層カーボンナノチューブ透明導電性薄膜上での骨芽細胞様細胞の培養
赤坂司, 阿部薫明, 宇尾基弘, 亘理文夫, 歯科材料・器械, 27, 2, 168, 168, 31 Mar. 2008
(一社)日本歯科理工学会, Japanese

シルクによる繊維強化レジンの作製とその機械的特性
宇尾 基弘, 阿部 薫明, 赤坂 司, 亘理 文夫, 歯科材料・器械, 27, 2, 125, 125, Mar. 2008
(一社)日本歯科理工学会, Japanese

骨関連細胞への生体材料の微細構造のin vitroでの影響(Effect of microstructures of biomaterials on bone-related cells in vitro)
リ・シャオミン, 宇尾 基弘, 赤坂 司, 亘理 文夫, 歯科材料・器械, 27, 2, 195, 195, Mar. 2008
(一社)日本歯科理工学会, English

各種マイクロ・ナノ粒子の生体内での分布状態の追跡とその可視化
阿部 薫明, 江崎 光恵, 赤坂 司, 宇尾 基弘, 久保木 芳徳, 亘理 文夫, 歯科材料・器械, 27, 2, 231, 231, Mar. 2008
(一社)日本歯科理工学会, Japanese

ナノチューブ系新素材をベースとした生体材料の放電プラズマ焼結
亘理文夫, 宇尾基弘, 赤坂司, 阿部薫明, 木村久道, 大森守, 大久保昭, 東北大学金属材料研究所金属ガラス総合研究センター共同利用研究報告書, 2007, 2008

ナノ微粒子の体内動態可視化法の開発 生体に投与した有機・無機粒子の体内動態のイメージング
亘理文夫, 阿部薫明, 森田学, 江崎光恵, 宇尾基弘, 赤坂司, 久保木芳徳, ナノ微粒子の体内動態可視化法の開発 平成19年度 総括・分担研究報告書, 2008

ナノ微粒子の体内動態可視化法の開発 細胞培養におけるカーボンナノチューブ膜の影響
亘理文夫, 宇尾基弘, 赤坂司, 阿部薫明, ナノ微粒子の体内動態可視化法の開発 平成19年度 総括・分担研究報告書, 2008

正常および癌肝細胞培養に対するCNTの影響の相違
伊藤佐智子, 赤坂司, 八若保孝, 亘理文夫, 歯科材料・器械, 27, 5, 2008

ナノ微粒子の体内動態可視化法の開発 カーボンナノチューブをコートしたシリコーンラバーでの細胞接着に関する研究
戸塚靖則, 高師則行, 赤坂司, 松岡真琴, 亘理文夫, ナノ微粒子の体内動態可視化法の開発 平成19年度 総括・分担研究報告書, 2008

生物学的反応プロセスによるナノ微粒子の機能性転換　　　　　　　　　　　　　　　
亘理 文夫, 横山 敦郎, Gelinsky M., 廖 素三, 井上 沙織, 赤坂 司, 日本バイオマテリアル学会大会予稿集, 29回, 220, 220, Nov. 2007
日本バイオマテリアル学会, Japanese

有機・無機微粒子の体内動態イメージング法の検討　　　　　　　　　　　　　　　
阿部 薫明, 小山 千佳, Rosca Iosif Danel, 赤坂 司, 宇尾 基弘, 久保木 芳徳, 亘理 文夫, 日本バイオマテリアル学会大会予稿集, 29回, 409, 409, Nov. 2007
日本バイオマテリアル学会, Japanese

カーボンナノチューブのアパタイト修飾
赤坂司, 阿部薫明, 宇尾基弘, 亘理文夫, 無機リン化学討論会講演予稿集, 17th, 104, 105, 02 Oct. 2007
Japanese

B28 Cell Culture on a Imogolite Scaffold
Ishikawa Kosuke, Akasaka Tsukasa, Yawaka Yasutaka, Suzuki Masaya, Watari Fumio, 粘土科学討論会講演要旨集, 51, 144, 145, 12 Sep. 2007
The Clay Science Society of Japan, Japanese

人工複合糖質高分子によるStreptococcus mutansの増殖阻害
赤坂司, 阿部薫明, 宇尾基弘, 亘理文夫, 北海道外科雑誌, 52, 1, 87, 87, 20 Jun. 2007
北海道外科学会, Japanese

耐磨耗性インプラント材料のためのチタンへの表面炭化物形成
朱 禹赫, 宇尾 基弘, 赤坂 司, 亘理 文夫, 北海道外科雑誌, 52, 1, 86, 86, Jun. 2007
北海道外科学会, Japanese

金属微粒子の体内動態の可視化と生体組織への影響
阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 小山 千佳, 久保木 芳徳, 北海道外科雑誌, 52, 1, 87, 87, Jun. 2007
北海道外科学会, Japanese

人工複合糖質高分子によるStreptococcus mutansの増殖阻害機構
赤坂司, 阿部薫明, 宇尾基弘, 亘理文夫, 歯科材料・器械, 26, 2, 245, 245, 25 Apr. 2007
(一社)日本歯科理工学会, Japanese

各種金属微粒子の体内動態のイメージング
阿部 薫明, 赤坂 司, 宇尾 基弘, 久保木 芳徳, 亘理 文夫, 歯科材料・器械, 26, 2, 241, 241, Apr. 2007
(一社)日本歯科理工学会, Japanese

カーボンナノチューブのバイオ応用
赤坂司, 青木尚史, 横山敦郎, 阿部薫明, 宇尾基弘, 亘理文夫, 日本顕微鏡学会北海道支部学術講演会プログラム・予稿集, 2006, 17, Feb. 2007
Japanese

X線分析顕微鏡及びEDSを用いた生体内金属元素の分布分析
宇尾基弘, 阿部薫明, 赤坂司, 亘理文夫, 日本顕微鏡学会北海道支部学術講演会プログラム・予稿集, 2006, 2007

蛍光エックス線顕微鏡を用いたナノマテリアルの体内動態の可視化
阿部薫明, 小山千佳, 赤坂司, 宇尾基弘, 久保木芳徳, 亘理文夫, 三学協会北海道支部研究発表会講演要旨集, 2007, 2007

ナノチューブ系新素材をベースとした生体材料の放電プラズマ焼結
亘理文夫, 宇尾基弘, 赤坂司, 阿部薫明, ZHU YH., WANG W., 横山敦郎, 木村久道, 大森守, 大久保昭, 東北大学金属材料研究所金属ガラス総合研究センター共同利用研究報告書, 2006, 2007

Na₂O-CaO-P₂O₅系水溶性ガラスの作製と細胞毒性評価
宇尾基弘, 赤坂司, 阿部薫明, 亘理文夫, 無機リン化学討論会講演要旨集, 17th, 2007

単分散カーボンナノチューブを用いたハイドロキシアパタイト合成
赤木健太郎, 赤坂司, 亘理文夫, 古月文志, 日本化学会北海道支部研究発表会講演要旨集, 2007, 2007

カーボンナノチューブ膜の作製と物性
宇尾基弘, 阿部薫明, 赤坂司, 亘理文夫, 歯科材料・器械, 26, 2, 2007

Handbook of Biomeneralization -Medical and Clinical Aspects　　　　　　　　　　　　　　　
Fumio Watari, Kazuchika Tamura, Atsuro Yokoyama, Ken-ichiro Shibata, Tsukasa Akasaka, Bunshi Fugetsu, Kiyotaka Asakura, Motohiro Uo, Yasunori Totsuka, Yoshinori Sato, Kazuyuki Tohji, 127, 144, 2007
Wiley-VCH, English, Introduction other

BTC(Bench to Clinic)研究室から臨床へ 口腔内における糖鎖の働き
赤坂司, 亘理文夫, DE, 159, 26, 28, 25 Oct. 2006
(一社)日本歯科理工学会, Japanese

人工複合糖質高分子によるStreptococcus mutansの増殖阻害
赤坂司, 阿部薫明, 宇尾基弘, 亘理文夫, 歯科材料・器械, 25, 5, 336, 336, 16 Oct. 2006
(一社)日本歯科理工学会, Japanese

バイオ応用へ向けた機能性フラーレンの作製
阿部 薫明, 赤坂 司, 宇尾 基弘, 亘理 文夫, 歯科材料・器械, 25, 5, 360, 360, Oct. 2006
(一社)日本歯科理工学会, Japanese

人工複合糖質高分子によるStreptococcus mutansの発育阻害
赤坂司, 宇尾基弘, 亘理文夫, フラーレン・ナノチューブ総合シンポジウム講演要旨集, 31st, 106, 12 Jul. 2006
Japanese

糖鎖工学と歯学
赤坂司, 宇尾基弘, 亘理文夫, 北海道歯学雑誌, 27, 1, 41, 44, 15 Jun. 2006
北海道歯学会, Japanese

歯科用合金粉末を含む口腔組織中のセレンの分布及び状態分析
宇尾 基弘, 赤坂 司, 亘理 文夫, 歯科材料・器械, 25, 2, 191, 191, Apr. 2006
(一社)日本歯科理工学会, Japanese

放電プラズマ焼結法により作製したインプラント用カーボンナノチューブ固化体の機械的性質および生体適合性
WANG W., 横山敦郎, 宇尾基弘, 赤坂司, 亘理文夫, 木村久道, 大森守, 大久保昭, 東北大学金属材料研究所金属ガラス総合研究センター共同利用研究報告書, 2005, 2006

歯科用合金を含む口腔組織中のセレンの分布と状態分析
宇尾基弘, 赤坂司, 亘理文夫, 朝倉清高, 日本顕微鏡学会北海道支部学術講演会プログラム・予稿集, 2005 (Web), 2006

XAFSを用いた金属インプラント周囲組織中の微量金属元素の状態分析
宇尾基弘, 赤坂司, 亘理文夫, 朝倉清高, 横山敦郎, 北海道外科雑誌, 51, 1, 2006

表面炭化したチタンの生体材料特性
ZHU YH., WANG W., 宇尾基弘, 赤坂司, 菅原敏, 横山敦郎, 亘理文夫, 歯科材料・器械, 25, 2, 2006

Comparison of Morphology and behavior of carbon nanotubes and asbestos　　　　　　　　　　　　　　　
F. Watari, M. Inoue, T. Akasaka, N. Sakaguchi, H. Ichinose, M. Uo, Proceedings of 6th Asian BioCeramics Symposium 2006, 142, 145, 2006
English, Introduction international proceedings

細菌付着阻害剤へ向けた糖鎖化合物の調製
赤坂司, 宇尾基弘, 菅原敏, 亘理文夫, 歯科材料・器械, 24, 5, 348, 348, 05 Sep. 2005
(一社)日本歯科理工学会, Japanese

カーボンナノチューブを用いた細胞培養用スキャホールドの開発
青木尚史, 横山敦郎, 赤坂司, 宇尾基弘, 亘理文夫, 歯科材料・器械, 24, 5, 281, 281, 05 Sep. 2005
(一社)日本歯科理工学会, Japanese

炭化炭素雰囲気中で表面炭化したチタンの耐磨耗性生体材料特性
朱 禹赫, 下妻 光夫, 宇尾 基弘, 赤坂 司, 菅原 敏, 王 蔚, 亘理 文夫, 歯科材料・器械, 24, 5, 292, 292, Sep. 2005
(一社)日本歯科理工学会, Japanese

希土類含有カーボンナノカプセルの物性と細胞毒性
宇尾 基弘, 赤坂 司, 亘理 文夫, 横山 敦郎, 田村 一央, 戸塚 靖則, 歯科材料・器械, 24, 5, 350, 350, Sep. 2005
(一社)日本歯科理工学会, Japanese

コバルトを添加した試作歯科用金合金の諸特性
石 典芳, 岩崎 佳治, 菅原 敏, 朱 禹赫, 赤坂 司, 宇尾 基弘, 亘理 文夫, 歯科材料・器械, 24, 5, 363, 363, Sep. 2005
(一社)日本歯科理工学会, Japanese

純チタンと歯科用貴金属合金のレーザー溶接
岩崎 佳治, 大川 昭治, 赤坂 司, 宇尾 基弘, 亘理 文夫, 日本歯技, 2003, 434, 33, 40, Jul. 2005
(公社)日本歯科技工士会, Japanese

カーボンナノチューブの細胞培養用担体への応用
青木尚史, 横山敦郎, 赤坂司, 宇尾基弘, 亘理文夫, 北海道外科雑誌, 50, 1, 78, 78, 20 Jun. 2005
北海道外科学会, Japanese

バイオミメティックコーティングを用いたカーボンナノチューブの表面修飾
赤坂司, 宇尾基弘, 亘理文夫, 北海道外科雑誌, 50, 1, 78, 78, 20 Jun. 2005
北海道外科学会, Japanese

生体用カーボンナノチューブ焼結体の作製と特性
王 蔚, 近藤 英臣, 横山 敦朗, 宇尾 基弘, 大川 昭治, 赤坂 司, 菅原 敏, 亘理 文夫, 大森 守, 北海道外科雑誌, 50, 1, 77, 78, Jun. 2005
北海道外科学会, Japanese

蛍光性を持つコンポジットレジン用ガラスフィラーに関する研究
宇尾 基弘, 赤坂 司, 亘理 文夫, 谷 和俊, 森田 学, 北海道外科雑誌, 50, 1, 78, 79, Jun. 2005
北海道外科学会, Japanese

焼結したカーボンナノチューブ固化体の生体適合性
王 蔚, 横山 敦郎, 廖 素三, 宇尾 基弘, 赤坂 司, 大川 昭治, 菅原 敏, 亘理 文夫, 大森 守, 歯科材料・器械, 24, 2, 135, 135, Apr. 2005
(一社)日本歯科理工学会, Japanese

純チタンと歯科用貴金属合金のレーザー溶接
岩崎佳治, 岩崎佳治, 大川昭治, 赤坂司, 宇尾基弘, 亘理文夫, 日本歯技, 434, 2005

難焼結性生体材料の放電プラズマ焼結とその評価-放電プラズマ焼結を用いたカーボンナノチューブ固化体の製作とその評価-
WANG W., 横山敦郎, LIAO S. S., 宇尾基弘, 赤坂司, 亘理文夫, 大森守, 大久保昭, 東北大学金属材料研究所金属ガラス総合研究センター共同利用研究報告書, 2004, 2005

チタンと歯科用貴金属合金の開先形態におけるレーザー溶接
岩崎 佳治, 大川 昭治, 宇尾 基弘, 赤坂 司, 中田 久夫, 朝田 英哉, 亘理 文夫, 日本歯科技工学会雑誌, 25, 2, 234, 234, Dec. 2004
(一社)日本歯科技工学会, Japanese

複合糖質高分子によるカーボンナノチューブの表面修飾
赤坂司, 亘理文夫, 日本バイオマテリアル学会シンポジウム予稿集, 2004, 134, 15 Nov. 2004
Japanese

カーボンナノチューブの生体材料への応用 細胞培養用担体の開発　　　　　　　　　　　　　　　
青木 尚史, 横山 敦郎, 川崎 貴生, 野田坂 圭伸, 赤坂 司, 宇尾 基弘, 亘理 文夫, 日本補綴歯科学会雑誌, 48, 112回特別, 151, 151, Oct. 2004
(公社)日本補綴歯科学会, Japanese

カーボンナノチューブの生体材料への応用―細胞培養用担体の開発―
青木尚史, 横山敦郎, 川崎貴生, 野田坂圭伸, 赤坂司, 宇尾基弘, 亘理文夫, 日本補綴歯科学会学術大会プログラム・抄録集, 112th, 151, Oct. 2004
Japanese

バイオミメティックコーティングを用いたカーボンナノチューブの表面修飾
赤坂司, 宇尾基弘, 大川昭治, 菅原敏, 亘理文夫, 歯科材料・器械, 23, 5, 392, 392, 10 Sep. 2004
(一社)日本歯科理工学会, Japanese

カーボンナノチューブ固化体の焼結と生体適合性
王 蔚, 近藤 英臣, 横山 敦郎, 川崎 貴生, 宇尾 基弘, 大川 昭治, 赤坂 司, 菅原 敏, 亘理 文夫, 大森 守, 歯科材料・器械, 23, 5, 330, 330, Sep. 2004
(一社)日本歯科理工学会, Japanese

チタンと貴金属とのレーザー溶接部の元素分布
岩崎 佳治, 大川 昭治, 宇尾 基弘, 赤坂 司, 菅原 敏, 亘理 文夫, 歯科材料・器械, 23, 5, 352, 352, Sep. 2004
(一社)日本歯科理工学会, Japanese

糖鎖によるカーボンナノチューブの表面修飾
赤坂司, 佐藤義倫, 田路和幸, 亘理文夫, 傾斜機能材料論文集, 2003, 116, 121, 15 Jul. 2004
Japanese

糖鎖によるカーボンナノチューブの表面修飾
赤坂司, 亘理文夫, 佐藤義倫, 田路和幸, 北海道外科雑誌, 49, 1, 100, 101, 20 Jun. 2004
北海道外科学会, Japanese

X線分析顕微鏡の生体内金属材料及び周囲組織分析への応用
宇尾 基弘, 赤坂 司, Daniel Rosca Iosif, 亘理 文夫, 北海道外科雑誌, 49, 1, 98, 98, Jun. 2004
北海道外科学会, Japanese

ナノ・マイクロ微粒子の生体への影響 歯科診療で発生する微粒子の細胞への影響
田村 一央, 高師 則行, ロスカ・イオシフ, 赤坂 司, 渥美 崇, 佐藤 義倫, 宇尾 基弘, 戸塚 靖則, 亘理 文夫, 歯科材料・器械, 23, 2, 129, 129, Mar. 2004
(一社)日本歯科理工学会, Japanese

カーボンナノチューブ焼結体の作製とその特性
王 蔚, 近藤 英臣, 横山 敦郎, 川崎 貴生, 宇尾 基弘, 大川 昭治, 赤坂 司, 菅原 敏, 亘理 文夫, 大森 守, 歯科材料・器械, 23, 2, 133, 133, Mar. 2004
(一社)日本歯科理工学会, Japanese

蛍光性を持つコンポジットレジン用ガラスフィラーに関する研究
宇尾 基弘, 赤坂 司, Daniel Rosca Iosif, 大川 昭治, 菅原 敏, 亘理 文夫, 谷 和俊, 森田 学, 歯科材料・器械, 23, 2, 135, 135, Mar. 2004
(一社)日本歯科理工学会, Japanese

X線分析顕微鏡の生体内金属材料及び周囲組織分析への応用
宇尾基弘, 赤坂司, DANIEL R I, 亘理文夫, 傾斜機能材料論文集, 2003, 2004

Tissue reaction to nanotube, nanoparticle, and microparticle, and their biological application. Preparation and evaluation of composite material using carbon nanotube.
宇尾基弘, 赤坂司, 亘理文夫, 横山敦郎, 川崎貴夫, 田村一央, 佐藤義倫, 田路和幸, 大森守, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 2004

Tissue reaction to nanotube, nanoparticle, and microparticle, and their biological application. Preparation and evaluation of composite material using carbon nanotube: biotoxicity of carbon nanocapsule.
宇尾基弘, 赤坂司, 横山敦郎, 川崎貴夫, 田村一央, 戸塚靖則, 佐藤義倫, 田路和幸, 大森守, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 2004

The tissue reaction of nanotube, nanoparticle, and microparticle, and biological application. Production and evaluation of composite material: biotoxicity of carbon nanocapsules.
亘理文夫, 宇尾基弘, 赤坂司, ダニエルロスカ イオシフ, 田村一央, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 2004

The tissue reaction of nanotube, nanoparticle, and microparticle, and biological application. Functionalization of carbon nanotube.
亘理文夫, 赤坂司, 宇尾基弘, 佐々木紘子, 青木尚志, 田村一央, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 2004

乾式合成法による窒化チタン/アパタイト系傾斜機能型インプラントの作製と評価
近藤英臣, 横山敦郎, 川崎貴生, 宇尾基弘, 大川昭治, 赤坂司, 菅原敏, 亘理文夫, 歯科材料・器械, 23, 2, 2004

Tissue reaction to nanotube, nanoparticle, and microparticle, and their biological application. Application of carbon nanotube to biomaterial.
横山敦郎, 山本悟, 赤坂司, 古月文志, 佐藤義倫, 田路和幸, 小野木伯薫, 橋田俊之, 大森守, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 2004

象牙質へのレジンモノマーの拡散と強化の可能性
神島奈穂子, 大畑昇, 宇尾基弘, 大川昭治, 赤坂司, 菅原敏, 亘理文夫, 歯科材料・器械, 23, 2, 2004

XAFSを用いた金属インプラント周囲組織の分析
宇尾基弘, 赤坂司, 亘理文夫, 横山敦郎, 田村一央, 戸塚靖則, 歯科材料・器械, 23, 5, 2004

The tissue reaction of nanotube, nanoparticle, and microparticle, and biological application. Functionalization of carbon nanotube.
AKASAKA TSUKASA, YOKOYAMA ATSURO, TAMURA KAZUCHIKA, UO MOTOHIRO, WATARI FUMIO, SATO YOSHITSUGU, TAJI KAZUYUKI, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 518, 531, 2004
Japanese

Tissue reaction to nanotube, nanoparticle, and microparticle, and their biological application. Functionalization of carbon nanotube and its biological application. Modification by carbohydrate compounds.
AKASAKA TSUKASA, SASAKI KOKO, AOKI HISASHI, YOKOYAMA ATSURO, TAMURA KAZUCHIKA, UO MOTOHIRO, WATARI FUMIO, SATO YOSHITSUGU, TAJI KAZUYUKI, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 628, 638, 2004
Japanese

Tissue reaction to nanotube, nanoparticle, and microparticle, and their biological application. Functionalization of carbon nanotube and its biological application. Plasticity of single-walled carbon nanotube.
SASAKI KOKO, AOKI HISASHI, YOKOYAMA ATSURO, KAWASAKI TAKAO, AKASAKA TSUKASA, UO MOTOHIRO, WATARI FUMIO, SATO YOSHITSUGU, TAJI KAZUYUKI, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成15年度 総括・分担研究報告書, 665, 667, 2004
Japanese

カーボンナノチューブを用いた培養基材の表面改質
赤坂司, 田村一央, 亘理文夫, 日本バイオマテリアル学会大会予稿集, 25th, 215, 16 Dec. 2003
Japanese

チタンと歯科用貴金属合金のレーザー溶接
岩崎 佳治, 大川 昭治, 宇尾 基弘, 赤坂 司, 中田 久夫, 朝田 英哉, 亘理 文夫, 日本歯科技工学会雑誌, 24, 2, 282, 282, Dec. 2003
(一社)日本歯科技工学会, Japanese

ナノ/ミクロ粒子に対する病理生物学的反応(Pathobiological response to nano/micro particles)
田村 一央, 高師 則行, 宇尾 基弘, ロスカイオシフ, 赤坂 司, 戸塚 靖則, 亘理 文夫, 歯科基礎医学会雑誌, 45, 5, 275, 275, Sep. 2003
(一社)歯科基礎医学会, English

象牙質へのカーボンナノチューブ吸着による表面改質
赤坂司, 宇尾基弘, 大川昭治, 菅原敏, 亘理文夫, 歯科材料・器械, 22, 5, 397, 397, 25 Aug. 2003
The Japanese Society for Dental Materials and Devices (JSDMD), Japanese

チタンと歯科用貴金属合金の開先形態におけるレーザー溶接　　　　　　　　　　　　　　　
岩崎 佳治, 大川 昭治, 宇尾 基弘, 赤坂 司, 菅原 敏, 亘理 文夫, 歯科材料・器械, 22, 5, 403, 403, Aug. 2003
(一社)日本歯科理工学会, Japanese

人工複合糖質高分子によるカーボンナノチューブの表面修飾
赤坂司, 宇尾基弘, 田路和幸, 亘理文夫, 日本糖質学会年会要旨集, 24th, 125, 03 Jul. 2003
Japanese

放電プラズマ焼結法で作製した窒化チタン/アパタイト系傾斜機能型インプラントの物性と生体適合性の評価
近藤 英臣, 横山 敦郎, 川崎 貴生, 宇尾 基弘, 大川 昭治, 菅原 敏, 赤坂 司, 亘理 文夫, 歯科材料・器械, 22, 2, 54, 54, Mar. 2003
(一社)日本歯科理工学会, Japanese

カーボンナノチューブとナノ・マイクロ微粒子に対する生体反応
田村 一央, 高師 則行, 赤坂 司, 宇尾 基弘, 大川 昭治, 菅原 敏, 古月 文志, 田路 和幸, 戸塚 靖則, 亘理 文夫, 歯科材料・器械, 22, 2, 57, 57, Mar. 2003
(一社)日本歯科理工学会, Japanese

歯質とレジン及びポーセレン修復部の識別に関する分光学的研究
谷 和俊, 森田 学, 宇尾 基弘, 大川 昭治, 赤坂 司, 菅原 敏, 亘理 文夫, 歯科材料・器械, 22, 2, 80, 80, Mar. 2003
(一社)日本歯科理工学会, Japanese

チタンと歯科用貴金属合金のレーザー溶接による接合
岩崎 佳治, 大川 昭治, 宇尾 基弘, 赤坂 司, 菅原 敏, 亘理 文夫, 歯科材料・器械, 22, 2, 122, 122, Mar. 2003
(一社)日本歯科理工学会, Japanese

Nd:YAG,Er:YAG,CO2レーザー照射した歯質の表面形態観察とスペクトル分析
ヤマダ・マギダ・キヨコ, 宇尾 基弘, 大川 昭治, 赤坂 司, 菅原 敏, 亘理 文夫, 歯科材料・器械, 22, 2, 124, 124, Mar. 2003
(一社)日本歯科理工学会, Japanese

各種レジンモノマーの歯質への拡散の測定
神島 奈穂子, 佐藤 範幸, 大畑 昇, 宇尾 基弘, 大川 昭治, 赤坂 司, 菅原 敏, 亘理 文夫, 歯科材料・器械, 22, 2, 131, 131, Mar. 2003
(一社)日本歯科理工学会, Japanese

チタンと歯科用貴金属合金のレーザー溶接による接合
岩崎佳治, 大川昭治, 宇尾基弘, 赤坂司, 菅原敏, 亘理文夫, 日本歯科理工学会学術講演会講演集, 41st, 2003

ナノ・マイクロ微粒子バイオマテリアルの組織障害の機序
田村一央, 高師則行, 赤坂司, IOSIF R, 宇尾基弘, 戸塚靖則, 亘理文夫, 日本バイオマテリアル学会大会予稿集, 25th, 2003

Nd:YAG,Er:YAG,CO₂レーザー照射した歯質の表面形態観察とスペクトル分析
ヤマダ(マギダ) キヨコ, 宇尾基弘, 大川昭治, 赤坂司, 菅原敏, 亘理文夫, 日本歯科理工学会学術講演会講演集, 41st, 2003

各種レジンモノマーの歯質への拡散の測定
神島奈穂子, 佐藤範幸, 大畑昇, 宇尾基弘, 大川昭治, 赤坂司, 菅原敏, 亘理文夫, 日本歯科理工学会学術講演会講演集, 41st, 2003

歯質とレジン及びポーセレン修復部の識別に関する分光学的研究
谷和俊, 森田学, 宇尾基弘, 大川昭治, 赤坂司, 菅原敏, 亘理文夫, 日本歯科理工学会学術講演会講演集, 41st, 2003

歯根膜由来細胞に対する微粒子の影響
田村一央, 高師則行, 赤坂司, 宇尾基弘, 大川昭治, 菅原敏, 戸塚靖則, 亘理文夫, 歯科材料・器械, 22, 5, 2003

乾式合成法によるTi-HAP複合体の作製と評価
近藤英臣, 横山敦郎, 川崎貴生, 宇尾基弘, 大川昭治, 赤坂司, 菅原敏, 亘理文夫, 歯科材料・器械, 22, 5, 2003

象牙質への各種レジンモノマーの浸透度の比較
神島奈穂子, 佐藤範幸, 大畑昇, 宇尾基弘, 大川昭治, 赤坂司, 菅原敏, 亘理文夫, 歯科材料・器械, 22, 5, 2003

放電プラズマ焼結法で作製した窒化チタン/アパタイト系傾斜機能型インプラントの物性と生体適合性の評価
近藤英臣, 横山敦郎, 川崎貴生, 宇尾基弘, 大川昭冶, 菅原敏, 赤坂司, 亘理文夫, 日本歯科理工学会学術講演会講演集, 41st, 2003

カーボンナノチューブとナノ・マイクロ微粒子に対する生体反応
田村一央, 高師則行, 赤坂司, 宇尾基弘, 菅原敏, 古月文志, 田路和幸, 戸塚靖則, 亘理文夫, 日本歯科理工学会学術講演会講演集, 41st, 2003

Tissue reactivity of nanotubes, nanoparticles, and microparticles, and their biological applications. Functionalization of carbon nanotubes and biological application.
亘理文夫, 赤坂司, 宇尾基弘, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成14年度 総括・分担研究報告書, 2003

Tissue reactivity of nanotubes, nanoparticles, and microparticles, and their biological applications. Functionalization of carbon nanotubes, and biological application.
AKASAKA TSUKASA, UO MOTOHIRO, WATARI FUMIO, TOJI KAZUYUKI, ナノチューブ、ナノ微粒子、マイクロ微粒子の組織反応性とバイオ応用 平成14年度 総括・分担研究報告書, 424, 425, 2003
Japanese

北海道産カラマツ由来多糖アラビノガラクタンの構造解析
赤坂司, 前川宜彦, 日本糖質学会年会要旨集, 23rd, 113, 25 Jul. 2002
Japanese

北海道産カラマツ由来多糖アラビノガラクタンの構造解析
赤坂司, 前川宜彦, 日本木材学会北海道支部講演集, 33, 57, 60, 15 Oct. 2001
Japanese

プラスミド‐糖鎖コンジュゲートを用いた遺伝子発現制御系の構築
林克洋, 赤坂司, 松浦和則, 小林一清, 高分子学会予稿集, 50, 5, 1005, 07 May 2001
Japanese

DNA‐糖コンジュゲートの創成
松浦和則, 赤坂司, 日比野美紀, 山田喜直, 小林一清, 日本糖質学会年会要旨集, 21st, 51, 30 Jun. 2000
Japanese

ジアゾカップリング法によるDNA‐シクロデキストリンコンジュゲートの合成と分子認識
林克洋, 赤坂司, 松浦和則, 小林一清, 高分子学会予稿集, 49, 5, 871, 12 May 2000
Japanese

Preparation of block-type of oligoDNA-glycopolymer conjugates and their characterization.
AKASAKA TSUKASA, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, 日本化学会講演予稿集, 78th, 2, 827, 15 Mar. 2000
Japanese

Facil Synthesis of Stable and Lectin-Recognizable DNA-Carbohydrate conjugates via Diazo Coupling (jointly worked)
Bioconjugate Chem , 11, 202-211, 2000

DNAハイブリダイゼーションを利用した糖クラスターの構築
松浦和則, 日比野美紀, 山田喜直, 赤坂司, 小林一清, 高分子学会予稿集, 49, 5, 2000

Preparation of block type of oligoDNA-glycopolymer conjugates.
AKASAKA TSUKASA, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, 日本化学会講演予稿集, 77th, 75, 10 Sep. 1999
Japanese

Preparation of Plasmid-Carbohydrate Conjugates and their Characterizations.
AKASAKA TSUKASA, MORI KOICHI, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, 高分子学会予稿集, 48, 4, 766, 12 May 1999
Japanese

Preparation of Plasmid-Carbohydrate Conjugates and their Characterizations.
AKASAKA TSUKASA, MORI KOICHI, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, EMI NOBUHIKO, 日本化学会講演予稿集, 76th, 2, 761, 15 Mar. 1999
Japanese

Synthesis of DNA-carbohydrate conjugate via diazocoupling: A new class of modified DNA with enhanced stability and lectin-recognition ability
K Matsuura, T Akasaka, M Hibino, K Kobayashi, CHEMISTRY LETTERS, 3, 247-248, 3, 247, 248, Mar. 1999
English

Synthesis of DNA-carbohydrate conjugate via diazocoupling: A new class of modified DNA with enhanced stability and lectin-recognition ability
K Matsuura, T Akasaka, M Hibino, K Kobayashi, CHEMISTRY LETTERS, 3, 247, 248, Mar. 1999
English

Synthesis and sspecific biological activities of polymethacrylate derivative having sulfated oligosaccharide side chains(jointly worked)　　　　　　　　　　　　　　　
J.Poly.Sci.A: Polym.Chem, 37, 789-800, 1999

DNAを基本骨格とした新規糖質高分子の分子設計
松浦和則, 赤坂司, 日比野美紀, 小林一清, 早川芳弘, 高分子学会予稿集, 48, 12, 1999

Synthesis of DNA-Sugar Conjugates and their Characterizations.
HIBINO MIKI, AKASAKA TSUKASA, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, 高分子学会予稿集, 47, 12, 3130, 3131, Sep. 1998
Japanese

Preparation of Plasmid-Sugar Conjugates and their Characterizations.
AKASAKA TSUKASA, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, EMI NOBUHIKO, 高分子学会予稿集, 47, 11, 2818, 2819, Sep. 1998
Japanese

Molecular Recognition of DNA-Sugar Conjugates.
AKASAKA TSUKASA, HIBINO MIKI, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, 高分子学会予稿集, 47, 4, 756, May 1998
Japanese

Synthesis of Sugar-DNA Conjugates by Diazocoupling and their Characterizations.
HIBINO MIKI, AKASAKA TSUKASA, MATSUURA KAZUNORI, KOBAYASHI KAZUKIYO, 高分子学会予稿集, 47, 4, 755, May 1998
Japanese

DNAへの糖鎖の導入による構造安定化,ヌクレアーゼ耐性,およびレクチン認識能の賦与
松浦和則, 赤坂司, 日比野美紀, 小林一清, バイオ・高分子シンポジウム講演要旨集, 8th, 1998

Synthesis of Polymethacrylates having a pendant maltoheptaose moiety by Radical Polymerization and Copolymerization of Methacryloyl maltoheptaoside Derivatives.
AKASAKA TSUKASA, YOSHIDA TAKASHI, URYU TOSHIYUKI, 高分子学会予稿集, 46, 2, 154, May 1997
Japanese

Synthesis of Polymethacrylates having a pendant maltoheptaose moiety by Radical Polymerization and Copolymerization of Methacryloyl maltoheptaoside Derivatives.
赤坂司, 吉田孝, 瓜生敏之, 高分子学会予稿集, 46, 2, 1997

Synthesis of Biological Polymethacrylates Having A Malto-oligosaccharide Chain By Radical Polymerization of Methacryloyl Maltoheptaose Derivative.
AKASAKA TSUKASA, YOSHIDA TAKASHI, URYU TOSHIYUKI, 高分子学会予稿集, 45, 7, 1223, 1224, Sep. 1996
Japanese

スタンダード歯科理工学 : 生体材料と歯科材料
新谷, 明一, 中嶌, 裕, 宮崎, 隆, 米山, 隆之, 石川, 邦夫, 赤坂, 司
学建書院, Mar. 2024, 9784762466144, x, 404p, Japanese

ナノインプリント技術ハンドブック
応用物理学会・ナノインプリント技術研究会, 13.6 マイクロ・ナノパターンの歯科バイオ応用
オーム社, Nov. 2019, 9784274224485, xiii, 781p, Japanese, [Contributor]

スタンダード歯科理工学 第７版　　　　　　　　　　　　　　　
赤坂 司, 15 接着・合着用材料
学建書院, Mar. 2019, 9784762456145, [Contributor]

スタンダード歯科理工学 第６版　　　　　　　　　　　　　　　
赤坂 司, １４ 接着・合着用材料
Mar. 2016, [Contributor]

動物細胞培養法　　　　　　　　　　　　　　　
赤坂 司, 第２章、第３節「カーボンナノチューブ薄膜上での細胞培養」
株式会社技術情報協会, 2014, [Contributor]

形状変形で収縮しフィットする歯のメッシュスーツを作る
科学研究費助成事業
27 Jun. 2025 - 31 Mar. 2028
赤坂 司
日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 25K22192

カーボンドットを応用した感染制御能と骨形成能を有する革新的GBR膜の開発
科学研究費助成事業
01 Apr. 2024 - 31 Mar. 2027
横山 敦郎, 赤坂 司, 山本 悟, 平田 恵理, 高野 勇太
日本学術振興会, 基盤研究(C), 北海道大学, 24K12996

天然歯・人工歯の表面へのパターン付与治療へ向けた歯周組織再生の形態ロジック構築
科学研究費助成事業
01 Apr. 2024 - 31 Mar. 2027
赤坂 司, 横山 敦郎, 吉村 善隆, 宮治 裕史, 牛島 夏未, 星 淡子
日本学術振興会, 基盤研究(B), 北海道大学, 23K28422

天然歯・人工歯の表面へのパターン付与治療へ向けた歯周組織再生の形態ロジック構築
科学研究費助成事業
01 Apr. 2023 - 31 Mar. 2027
赤坂 司, 横山 敦郎, 吉村 善隆, 宮治 裕史, 牛島 夏未, 星 淡子
日本学術振興会, 基盤研究(B), 北海道大学, 23H03733

マイクロ・ナノパターンを用いて歯髄幹細胞より誘導する歯牙および歯周組織再生の試み
科学研究費助成事業
01 Apr. 2023 - 31 Mar. 2026
飯田 俊二, 赤坂 司
概要として、１）パターン化研究材料の製造で使用する基本パターンを、基本形状としてピラー、グルーブ、ホールを100nm～50μmスケールでパターン化した。
２）基礎分化誘導に関しては、ヒト脱落乳歯歯髄幹細胞（SHED）、Saos-2細胞（ヒト骨芽細胞様骨肉腫細胞）、MG63細胞（ヒト骨肉腫細胞）を用いて骨芽細胞への分化誘導を行った。増殖後に細胞を計数して、細胞染色用として、またRNA抽出用として各細胞を播種し。播種後培養した。その後分化誘導培地にて１週間ごとに細胞を回収しながら４週間培養した。アリザリンレッド染色による石灰化の検出は１週間ごとに回収した細胞を固定した。固定後にアリザリンレッド染色液を入れて観察した。定量PCR法による骨芽細胞分化マーカーの検出は、回収した細胞のRNAを抽出し抽出したRNAをテンプレートにしてcDNAを合成した。
結果は分化誘導培地にて培養した細胞は、細胞の分布がまだらであった。分化誘導培地で培養した各細胞をアリザリンレッド染色したところ、SHEDでは１週間で強く染色された。また２週間培養したところ強く染色され3、4週間でも同様であり、今回の培養条件ではSHEDは2週間程度で石灰化がみられた。Saos-2細胞では、SHEDより石灰化が早く１週間で強く染色された。MG63細胞はどの時点でもほとんど染色されておらず、今回の培養条件では石灰化が進まない結果となった。分化誘導前（0d）および分化誘導2週間後（14d）の各細胞からRNAを抽出し、抽出液のRNA濃度を測定した。得られたRNA量はいずれの細胞も分化誘導されるとRNA収量が減少する傾向があり、特にSHEDでは得られるRNA量が少なかった。細胞検出には今後蛍光標識プローブを用いるものと、SYBR Greenをもちいるもので検出感度を比較して感度を上げるようにする予定である。
日本学術振興会, 基盤研究(C), 北海道大学, 23K09211

マイクロ・ナノパターンの力で誘導・分化させる正常な構造を有する象牙質の再生
科学研究費助成事業
01 Apr. 2023 - 31 Mar. 2026
吉田 靖弘, 赤坂 司
日本学術振興会, 基盤研究(C), 北海道大学, 23K09228

歯の表面に貼り付けて使うセメント質と歯根膜の形状と成分を疑似した人工歯根膜の開発　　　　　　　　　　　　　　　
科学研究費助成事業 挑戦的研究(萌芽)
30 Jun. 2022 - 31 Mar. 2025
赤坂 司
日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 22K18933

２段階薬剤放出性第４世代スキャフォールドの歯周組織再生への応用
科学研究費助成事業 基盤研究(C)
01 Apr. 2022 - 31 Mar. 2025
西田 絵利香, 宮治 裕史, 大矢根 綾子, 中村 真紀, 赤坂 司
アパタイト2層構造化による２段階薬剤放出性第４世代スキャフォールド構築のため，初年度はまず1層目の構築・評価を行った．
1層目として，アパタイトの難溶化（溶解性低下）と抗菌性の期待される生体微量元素（フッ素）を種々の濃度（0～1000 uM）でCaP過飽和溶液に添加し，スキャフォールド上にフッ素含有アパタイト層を作製した．
その結果，スキャフォールド内部および外部表面への均一なフッ素含有アパタイト層の生成を確認し，過飽和溶液へのフッ素添加濃度の増加により生成するアパタイト層のフッ素置換率（F/P比で評価）向上が認められた．
成膜後のスキャフォールドを生食に浸漬して溶解性等を評価したところ，過飽和溶液へのフッ素添加濃度の増加に伴い，生食中へのアパタイトの溶解量（7日後）は減少した．アパタイト結晶にフッ化物イオンが取り込まれることにより，層の難溶化したと考えられる，また，生食中へのフッ素溶出量も増加した．
以上より，フッ化物イオンをバイオミメティックコーティング法によりアパタイト層に取り込むことに成功した．フッ化物濃度の増加とともにアパタイト層の溶解度が低下し，減少した．
日本学術振興会, 基盤研究(C), 北海道大学, 22K10012

Development of micro/nano-patterns to create periodontal ligament sheets with three-dimensionally structures by guiding stem cells
Grants-in-Aid for Scientific Research
01 Apr. 2020 - 31 Mar. 2023
Yoshida Yasuhiro
Embryonic stem cells (ES cell) and deciduous dental pulp stem cells (SHED) were focused on as stem cells and their behaviors on various micro/nano-patterns were investigated. Mouse ES cells were cultured on micropatterns and immunostained for differentiation markers in the three germ layers. In most patterns, the order was ectoderm > endoderm > mesoderm, but the proportion of differentiation into endoderm was slightly increased in some of the patterns below 2μm. Cell behaviors on the SHED micropatterns were then investigated. There was no difference in the number of adherent cells in cell adhesion assay, but in cell proliferation and calcification, promotion was observed in specific patterns. These results suggest that the behaviors of stem cells may be controlled by the design of patterns.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20K10006

超立体微細パターン付与だけで歯根膜再生を可能とするインプラント周囲炎治療技術開発
科学研究費助成事業 基盤研究(B)
01 Apr. 2019 - 31 Mar. 2023
赤坂 司, 横山 敦郎, 吉村 善隆, 牛島 夏未, 宮治 裕史, 星 淡子, 加我 公行, 玉井 美保
日本学術振興会, 基盤研究(B), 北海道大学, 19H04461

Development of intelligent carbon nanomaterials for innovative biomaterials for oral region
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2019 - 31 Mar. 2022
YOKOYAMA ATSURO
In this study, application of carbon nanomaterials (CNMs) for drug carrier and controlling release of drug, surface modification of Ti with CNMs, and production of CNMs based membrane were done to develop the intelligent carbon nanomaterials (i-CNMs) with high function capable of controlling its expression. Antibiotics was loaded to CNMs. It was able to control the expression of its function by photoirradiation. Also, acceleration of bone formation was observed around Ti by surface modification of Ti with CNMs. CNMs based membrane showed excellent shielding property and maintaining the space for GBR membrane. These results suggested the invention of i-CNMs and its possibility of application for biomaterials.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 19H03839

歯科応用へ向けた骨芽細胞機能評価スクリーニング用マイクロ・ナノパターンの開発　　　　　　　　　　　　　　　
2020年度 物質・デバイス領域共同研究拠点
Mar. 2020 - Mar. 2022
赤坂 司, 伊東 駿也, 中川 勝, 宮内 昭浩
物質・デバイス領域共同研究拠点, 北海道大学、東北大学、東京医科歯科大学, Principal investigator

生体応用へ向けた細胞機能評価スクリーニング用マイクロ・ナノパターンの試作　　　　　　　　　　　　　　　
2019年度 生体医歯工学共同研究拠点共同研究プロジェクト
Jun. 2019 - Mar. 2022
赤坂 司, 中川 勝, 伊東 駿也, 宮内 昭浩
生体医歯工学共同研究拠点, 北海道大学、東北大学、東京医科歯科大学, Principal investigator

歯表面で歯医者自身が直接構築できる微細パターンによる歯周病治療法の開発　　　　　　　　　　　　　　　
科研費 挑戦的研究（萌芽）
Apr. 2018 - Mar. 2021
赤坂 司
文部科学省, Principal investigator, Competitive research funding

Development of smart nano-biomaterials and investigation of possibility on their clinical application for dentistry
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2016 - 31 Mar. 2019
Yokoyama Atsuro, Bianco Alberto, Takada Sari, Maeda Yukari, Kimura Sadahito
In this study, in order to provide the carbon nanomaterials (CNMs) for clinical application of the dentistry, we observe the biodegradability of carbon nanohorns (CNHs) and modify antibiotics (minocycline) and growth factors (bone morphogenetic proteins) to CNHs. The control of sustained release by LED irradiation of them and their effects were confirmed. Furthermore, we developed titanium with CNHs modified on the surface. We also observed in vivo kinetics of carbon nanotubes (CNTs) locally implanted in mice and suggested that CNTs remain at the site of implantation and do not accumulate in detectable quantities in other organs. These proof-of-concepts will promote future studies on the application of CNMs as smart Nano-Biomaterials which are multifunctionalized for the dentistry.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 16H05518

マイクロブラシ状コラーゲンからなる人工歯根膜を持つインプラントの開発　　　　　　　　　　　　　　　
平成２９年度調査研究助成金
Dec. 2017 - Nov. 2018
赤坂 司
公益財団法人鈴木謙三記念医科学応用研究財団, Principal investigator, Competitive research funding

Development of the therapeutic method for peri-implantitis using thin membranes with micro-nano structures
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
01 Apr. 2014 - 31 Mar. 2017
Atsuro Yokoyama
In this study, a nano imprint method was used to develop gelatin and titanium sheets with grooved and pillared structures. We investigated the effects of surfaces with micro and nano structures on adhesion and proliferation of osteoblasts, fibroblasts, and epithelial cells. However micro and nano structures accelerated adhesion and proliferation of osteoblasts and fibroblasts, those prevented epithelial cells. These results suggested that the micro and nano grooved and pillared structures effect on adhesion and orientation of osteoblasts, fibroblasts and epithelial cells.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 26670829

歯肉退縮を治療するバイオミメティック次世代歯科治療の開発　　　　　　　　　　　　　　　
研究助成
Apr. 2016 - Mar. 2017
赤坂 司
（公）寿原記念財団, Principal investigator, Competitive research funding

三次元構造再生を目指した歯周組織再生用マイクロ・ナノパターン化シートの開発
科学研究費補助金（基盤研究Ｃ）
Apr. 2013 - Mar. 2017
赤坂 司
文部科学省, Principal investigator, Competitive research funding

Development and evaluation on safety of Hybrid nano biomaterial for reconstruction for oral and maxillofacial region
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2013 - 31 Mar. 2016
YOKOYAMA ATSURO, AKASAKA Tsukasa, SATO Yoshinori, YAMAMOTO Satoru, TAKITA Hiroko, YUDASAKA Masako, SAKAGUCHI Norihito, SHIBA Kiyotaka
Effects of CNHs on bone formation, surface modification of CNTs and CNHs on titanium and their characteristics on bone formation, and the distribution of CNHs to internal organs used for biomaterial locally were evaluated in this study. CNHs accelerated differentiation of bone marrow cells to osteoblasts by Oncostatin M. The surface modification with CNHs and CNTs increased osteogenesis on titanium. CNHs have not been distributed in other organs on the local delivery.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 25293389

歯肉退縮を止めるための北海道産材料による歯科用マイクロ・ナノパターンシートの開発　　　　　　　　　　　　　　　
研究助成<一般>
Oct. 2014 - Mar. 2015
赤坂 司
秋山記念生命科学振興財団, Principal investigator, Competitive research funding

歯肉退縮を防止する歯表面でのマイクロ・ナノパターン直接形成による新規治療法の開発　　　　　　　　　　　　　　　
A-STEP 探索タイプ
Apr. 2014 - Mar. 2015
赤坂 司
科学技術振興機構(JST), Principal investigator, Competitive research funding

マイクロ・ナノパターン化バイオマテリアル上での細胞培養　　　　　　　　　　　　　　　
平成25年度研究設備の試行的利用
Apr. 2013 - Mar. 2014
赤坂 司
ナノテクノロジープラットフォーム, Principal investigator, Competitive research funding

電気刺激可能なナノ表面構造による細胞機能の制御　　　　　　　　　　　　　　　
医学系研究奨励
Apr. 2011 - Mar. 2014
赤坂 司
武田科学振興財団, Principal investigator, Competitive research funding

カーボンナノチューブの細胞膜透過特性を利用した抗癌治療の応用開発
科学研究費助成事業 特別研究員奨励費
2012 - 2013
赤坂 司, 亘理 文夫, CHEN X.
抗癌剤を長間使用すると多剤耐性が発現し、次第に抗癌剤の薬効が減衰する。抗癌剤の作用を保持し癌化学療法の治療効果を上げるには、この多剤耐性を抑制する必要がある。本研究では、代表的なナノマテリアルであるカーボンナノチューブ(CNT)の糖鎖・蛋白質吸着性、細胞膜透過性というユニークな特性に着目し、多剤耐性原因因子の吸着・制御による抗癌治療の高効率化を目的とした。今年度は、2種類のナノカーボン、(1)CNTと(2)ナノダイアモンド(ND)について抗癌薬剤への応用研究を行い、それぞれ新しい進展が得られた。
(1)CNTの多剤耐性抑制効果 : 癌細胞から薬剤を細胞外へ早期に排出させ、抗癌剤の細胞内滞留時間を低減し薬効を減じ、多剤耐性の原因となる因子として細胞膜のポンピング作用を司る蛋白質P-グリコプロテイン(PGP)や多剤耐性関連蛋白質(MRP)がある。CNTを投与すると透過型電子顕微鏡(TEM)観察から細胞内取込が確認され、投与量とともに、m-RNA, PGP総蛋白量、免疫染色のいずれのレベルでもこれらの蛋白量の低下が認められた。これはCNTの抗癌剤の多剤耐性抑制効果のメカニズムの直接的な解明である。
(2)NDのDDS応用のためのステルス性付与 : 現在、開発中のドラッグデリバリーシステム(DDS)においてはターゲット癌組織に至る前に、マクロファージ等の免疫細胞により異物と見なされ捕捉貧食されてれしまうという問題点がある。この問題をいかに回避するかというのは現時点における課題であるが、今回薬剤担体として用いたナノダイアモンドについて表面をポリグリセロール(PG)でコーティングすることにより、異物と認識されず貪食を回避できるステルス性を獲得することが示された。これはDDSの臨床応用にきわめて重要である。
日本学術振興会, 特別研究員奨励費, 北海道大学, 12F02108

Development of control technique of iPS cell behavior using CNT-coated electrode for regeneration of tooth
Grants-in-Aid for Scientific Research
2010 - 2012
AKASAKA Tsukasa
For control of functions of iPS cells, chamber with carbon nanotubes (CNTs) electrode was prepared. CNT electrode could maintain undifferentiated state of mouse iPS cells by adjustment of coating and type of CNTs. Patterns of CNT electrode could be printed repeatedly with ink jet printer using CNT inks. The patterns of CNT electrode enabled an arrangement of mouse iPS cells by technique of dielectrophoresis.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 22791919

Reconstruction of Jaw Bone with 3 dimension scaffold treated by functionalized carbon nano-material
Grants-in-Aid for Scientific Research
2010 - 2012
YOKOYAMA Atsuro, AKASAKA Tsukasa, YAMAMOTO Satoru, TAKITA Hiroko, SATO Yoshinori, YASUDA Motoaki, SHIBA Kiyotaka, YUDASAKA Masako
Functionalized carbon nanomaterials were developed by covalent bond of b-FGF to multiwalled carbon nanotubes and absorption of simvastatin to carbon nanohorns, and effects of them on bone formation were evaluated by animal experiments. Extensive bone formation was observed in the scaffolds with functionalized carbon nanomaterials in comparison with normal carbon nanomaterials. Also, biocompatibility carbon nanomaterials was observed by animal experiments for a long term.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 22390361

微粒子・ナノ材料を応用した石灰化誘導能を有する自己修復材料の開発
科学研究費補助金(基盤研究(C))
2010 - 2012
加我 正行, 橋本 正則, 赤坂 司
機能性ガラスであるS-PRG緻粒子を添加したシーラント材と充填材料(グラアイオノマーセメントとレジン)から溶出するアルカリ性イオンの種類と溶出量を測定し、それらの酸緩衝能を調べた。硬化した試料を乳酸溶液に浸漬するとイオン溶出効果が発現した。歯質がう蝕になる環境は、う蝕原因菌の酸産生によりpHが5.5以下になるとハイドロキシアパタイトが分解される。ここでアルカリ性イオンを放出する材料により脱灰が抑制され、再石灰誘導が生じる。この作用は材料は本研究で使用したバイオグラス、MTA等にもみられた。これら材料の配合比率を検索しながら再石灰化誘導の実験を行った。象牙質切片と微粒子を添加した実験材料との間に10μmのスペースを作り両端を固定して、人工唾液と蒸留水中に37℃で長期間保存し、象牙質切片表面上の析出物、結晶成分のSEM観察とエックス線回折とレーザーラマン分光光度計による同定を行った。試料から溶出するアルカリ性イオンや抗菌性のイオン、燐酸アパタイトの構成成分のカルシウムが再石灰化誘導に関与してる様子がわかり、人口唾液に浸漬した場合に析出物が6ヶ月目でわずかに観察された。SEM観察によりフラットな円盤状象牙質表面に針状の結晶が観察された。EDXによる析出物の同定から主に燐酸カルシウムと判断できた。長期的な展望に立った研究が必要である。実験試料とヒト抜去歯の接着強度の測定を微小引張試験(マイクロテンサイル)方法で行った。S-PRGを含有したレジンは歯質との接着方法の工夫により安定した接着強度が得られた。
文部科学省, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 22592273

Development of nanofiber reinforced resin using various nanotubes
Grants-in-Aid for Scientific Research
2009 - 2011
UO Motohiro, AKASAKA Tsukasa, ABE Shigeaki
Carbon nanotubes(CNTs) are fibrous crystalline material which have several to several ten nanometers in diameter. CNTs shows high mechanical strength close to the theoretical value, then the application as the structural material. In this study, preparation of the monolith and composite of CNTs and their application for dental and medical materials were studied. CNTs were successfully sintered using the spark plasma sintering and CNTs monolith showed similar elastic modulus to bone. CNTs/collagen composite films were also prepared. The film shows good tensile strength and flexibility.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, 北海道大学, Coinvestigator not use grants, Competitive research funding, 21659449

iPS細胞の未分化培養に有効な北海道産材料を用いた培養基材の探索　　　　　　　　　　　　　　　
研究開発助成事業 （タレント補助金）
Oct. 2009 - Mar. 2010
赤坂 司
ノーステック財団, Principal investigator, Competitive research funding

シリコーンゴム剤における細胞培養機能の評価　　　　　　　　　　　　　　　
ものづくり中小企業製品開発等支援補助金
Apr. 2009 - Mar. 2010
赤坂 司
全国中小企業団体中央会, Principal investigator, Competitive research funding

カーボンナノチューブ透明導電性薄膜での近赤外照射による殺菌　　　　　　　　　　　　　　　
研究開発助成事業 （タレント補助金）
Oct. 2008 - Mar. 2009
赤坂 司
ノーステック財団, Principal investigator, Competitive research funding

カーボンナノチューブコート細胞培養用ディッシュの新たな機能探索　　　　　　　　　　　　　　　
総長室重点配分経費 （若手研究者自立支援）
Jul. 2008 - Mar. 2009
赤坂 司
北海道大学, Principal investigator, Competitive research funding

単層CNTを利用した新規細胞培養方法の開発　　　　　　　　　　　　　　　
産学共同シーズイノベーション化事業（顕在化ステージ）
Apr. 2008 - Mar. 2009
赤坂 司
科学技術振興機構(JST), Principal investigator, Competitive research funding

Oral Tissue Reconstruction with 3D Scaffolds and Surface Modification Using Carbon Nanotubes
Grants-in-Aid for Scientific Research(基盤研究(B))
2007 - 2009
Atsuro YOKOYAMA, Tsukasa AKASAKA, Yoshinori SATO, Satoru YAMAMOTO, Motoaki YASUDA, Masanobu SHINDO, Fumihiro HIGASHINO
In this study, we developed 3D collagen scaffolds coated with carbon nanotubes to reconstruct jaw bone. It was shown that initial adhesion and spreading of osteoblastic cells on 3D collagen scaffold was accelerated by CNTs coating, especially center part of scaffold. Also, it was suggested to apply CNTs for reconstruction of jaw bone, because excellent osteoconductivy was observed when 3D collagen scaffolds coated with CNTs were implanted in bone.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 19390488

Chemical state analysis of rarely contained biological metallic element using X-ray absorption fine structure (XAFS) analysis
Grants-in-Aid for Scientific Research(基盤研究(B))
2006 - 2009
Motohiro UO, Kiyotaka ASAKURA, Fumio WATARI, Atsuro YOKOYAMA, Tsukasa AKASAKA, Shigeaki ABE
In this study, the chemical state of metallic elements and micro foreign bodies contained in the biological tissues were analyzed using XAFS method and the behavior of dental and medical alloys (e.g.titanium, nickel-titanium alloy, chromium-based alloy) was evaluated. Titanium in the surrounding tissues of dental and medical implants was usually existed as the metallic Ti, but, the titanium oxide was also detected. The titanium oxide was considered as derived from the dissolved and precipitated Ti. Nickel was existed as the ionic state in the surroundings of nickel-based alloy, then, the toxic effect of Ni ion to the surrounding tissues was concerned. In addition, trace element in the tussues (e.g.Se) analysis and micro XAFS analysis were also carried. In conclusion, the possibility of XAFS analysis for the biological tissue was suggested.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 18390509

カーボンナノチューブによるミュータンス菌の除菌および殺菌効果　　　　　　　　　　　　　　　
基盤的研究開発育成事業補助金 （若手研究補助金）
Oct. 2007 - Mar. 2008
赤坂 司
ノーステック財団, Principal investigator, Competitive research funding

人工複合糖質高分子およびレクチンを用いた口腔内細菌が認識する糖鎖構造の解明　　　　　　　　　　　　　　　
研究助成 （奨励助成）
Aug. 2006 - Mar. 2007
赤坂 司
秋山記念生命科学振興財団, Principal investigator, Competitive research funding

カーボンナノチューブ(CNTs)の生物学的特性を応用した再生療法の開発
科学研究費補助金(萌芽研究)
2005 - 2006
横山 敦郎, 赤坂 司, 進藤 正信, 山本 悟
本年度においては、多層カーボンナノチューブ(MWCNTs)を吸着することにより作製したスキャホールド上で骨髄細胞をデキサメサゾン(Dex)で骨芽細胞に誘導し、遺伝子発現に及ぼすMWCNTsの影響を検索するとともに、Dex添加と非添加の条件で骨髄細胞を培養したスキャホールドを、細胞を付着させた状態でラットの皮下組織に埋入した。
ラット骨髄細胞のDex誘導後1、3および7日の遺伝子の発現を、MWCNTsを吸着したスキャホールドで培養した場合とポリスチレンディッシュ上で培養した場合について、マイクロアレイを用いて比較検討した。全期間を通してMWCNTs固着スキャホールドで、インターロイキンなどの炎症に関与する遺伝子の発現が強く認められ、1、3日では、Matrix Gra ProteinやInsulin like Growth Factorなどの骨に関連する遺伝子の発現が、また7日ではインテグリンなどの接着に関与する遺伝子の発現が強く認められた。この結果から、MWCNTsは起炎性を有するが、骨髄細胞をより速く骨芽細胞に分化・誘導する可能性が示唆された。骨髄細胞をMWCNTs上でDexを添加して2週間培養した後、ラット皮下組織に6週間埋入すると、骨組織様の構造が認められたが、Dexを添加せずに培養した場合は観察されなかった。これらのことから、Dexを添加しMWCNTs上で骨髄細胞を培養すると骨芽細胞に分化し、皮下組織内で骨組織を形成する可能性が示唆された。
以上の結果から、MWCNTsをスキャホールドに用いると、骨髄細胞を強固にスキャホールドに付着した状態で骨芽細胞への分化を促進し、さらにこのスキャホールドは軟組織内で骨組織を形成する可能性が示された。
文部科学省, 萌芽研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 17659602

歯科用糖鎖ナノ材料の調製および歯周病細菌との相互作用
科学研究費補助金(若手研究(B))
2004 - 2006
赤坂 司
歯周病の原因である歯周病細菌の歯への定着を阻害する歯科用糖鎖ナノ材料の開発を目的とし、カーボンナノチューブやその糖鎖修飾体とし、効率的に歯周病細菌へ結合する歯科用ナノ糖鎖材料を開発する。
糖鎖自体の効果をより明白とするため、ポリスチレンディッシュに様々な糖鎖構造を持つ人工複合糖質高分子を吸着させ、基盤表面へのStreptococcus mutans(S.mutans)の吸着を検討した。その結果、ラクトースやキトビオース構造に対して付着し、さらにキトビオース構造は、S.mutansの増殖を抑制することが明らかとなった。
カーボンナノチューブによる口腔内細菌の捕捉を効率化するため、カーボンナノチューブの二次的な形態(直鎖状および球状)による菌体吸着の影響を検討した結果、両方の形態にて細菌捕捉が効率的に起こるものの、直鎖状のカーボンナノチューブの方がより効率的に菌体を吸着することが分かった。また、カーボンナノチューブにより吸着された細菌は引き続き培養を行うと細菌は増殖する事より、カーボンナノチューブによる相互作用自体は殺菌的ではなく吸着による除菌的効果であることが分かった。
以上の結果より、付着能や増殖抑制能を持つ糖鎖により修飾された直鎖状のカーボンナノチューブ網による細菌の捕捉が効率的であると考えられる。今後、カーボンナノチューブ自体に条件付与にて殺菌能の発現などの更なる効率化・機能付与化が望まれる。
文部科学省, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 16791177

Development of regenerative therapy for jaw bone using scaffold with carbon nanotubes.
Grants-in-Aid for Scientific Research(基盤研究(B))
2004 - 2006
Atsuro YOKOYAMA, 安田 元昭, 赤坂 司, 進藤 正信, 堀内 留美
In this study, investigation of biocompatibility of carbon nanotubes (CNTs), cell culture on the scaffold using with CNTs and fabrication of bulk CNTs were carried out for the purpose of reconstruction of jaw bone using with CNTs.
CNTs were implanted in the subcutaneous tissue in rats for 2 years, and histological and ultramicrostructural investigation to CNTs were done. Some CNTs were observed in lysosome in macrophages and only slight granulomatous inflammatory response was recognized around CNTs at 2 years, although granulation tissue with phagocytes was seen around CNTs at early stage after implant. The degree of aggregation of CNTs became to slight with time. Most of typical structure of CNTs like tubes was not changed after 2 years by observation using TEM. These results suggested that CNTs was not strong prophlogistic substance and structure of crystal of CNTs was stable in vivo.
Scaffolds with CNTs were made by vacuum filtration of the dispersed CNTs slurry onto polycarbonate (PC) membrane. Saos-2 (human osteoblast-like cell) was cultured on the scaffolds. Proliferation and alkaline phosphatase activity of Saos-2 cultured on the CNTs scaffolds were higher than those of PC membrane. Application of the CNTs scaffold for guided bone regeneration membrane to bone defects made on rat parietal bone was effective in osteogenesis compared to PC membrane.
Bulk CNTs materials were fabricated by spark plasma sintering (SPS). Physical and mechanical properties of bulk CNTs were close to those of bone, and biocompatibility was excellent. These results showed that bulk CNTs made by SPS method was suitable for bone substitute materials.
From these results, it was suggested that regenerative therapy for jaw bone using with CNTs would be possible.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 16390549

Biological applications of carbon nanotubes　　　　　　　　　　　　　　　
Funded Research
2002 - 2004
Competitive research funding

Effect of particle size on biocompatibility and biochemical evaluation of properties for various materials
Grants-in-Aid for Scientific Research(基盤研究(B))
2002 - 2003
Fumio WATARI, 高師 則行, 戸塚 靖則, 宇尾 基弘, 横山 敦郎, 赤坂 司
[Purpose]In dental material the powder/liquid method is abundantly used and abrasion powder arises in the sliding part of artificial joint. To study the effect of powders on health the size dependence of fine particles on biocompatibility was investigated by cell functional test and implantation test.
[Method] Monodispersed particles were extracted by sedimentation for the size 0.5, 3, 10μm and by ultrafiltration for less than 300nm. The particles were thus prepared with the same size for different materials.
[Size dependence] In cell functional test using the human neutrophil, periodontal ligament cell and rat macrophage, the cell survival rate was lowered and the emission of LDH, oxygen radical, cytokines TNF-α, IL-1β, IL-8, M-CSF increased as particle size became smaller, for Ti, Fe, Ni(each 500nm ; 3,10, 50, 150μm), TiO_2(30, 60, 300, 500nm ; 2μm), polylactic acid (1, 13μm). Especially, for the case of smaller than 10μm where phagocytosis was induced, the effect was remarkably pronounced. The stimulatibility of these effects is lower by about 1/100〜1/1000 than the intracellular toxicity.
[Material dependence]Both Ti whose ionic dissolution was negligible, confirmed by the ICP elemental analysis, and Fe, much more dissolvable, showed the similar size dependency. In Ni, cell survival rate was lower, emission of oxygen radical and cytokine was larger, the cell destruction was observed by microscopy, which was due to the stronger cytotoxicity of Ni.
[Conclusion]In animal implantation test, Ti particles showed biocompatibility for the size larger than about 100μm, and inflammation for smaller than 50μm. For less than 10μm phagocytosis was induced and inflammation was continued for the long term. For the biocompatibility in the material with a macroscopic size the material-dependent, chemical effect through dissolved ions is generally dominant. For the range of μm〜nm, the material-independent, physical size effect origined from the relative size relation between particles and cell/tissue becomes tangible, even for the bioactive and bioinert materials.
Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 14370623

細胞培養容器およびその製造方法
Patent right, 橋本 剛, 赤坂 司, 横山 敦郎, 亘理 文夫, 株式会社名城ナノカーボン, 国立大学法人 北海道大学
特願2007-323658, 14 Dec. 2007
特開2009-142218, 02 Jul. 2009
特許出願2007-323658, 200903090569528237

透明導電性薄膜およびその製造方法
Patent right, 橋本 剛, 赤坂 司, 横山 敦郎, 亘理 文夫, 株式会社名城ナノカーボン
特願2007-202344, 05 Jul. 2007
特開2009-016326, 22 Jan. 2009
特許出願2007-202344, 200903019440058521

DNA糖鎖コンジュゲートおよびその製造方法
Patent right, 小林 一清, 松浦 和則, 赤坂 司, 日比野 美紀, ヘキスト リサーチ アンド テクノロジー株式会社
特願平10-255343, 09 Sep. 1998
特開2000-086693, 28 Mar. 2000
特許公開2000-086693, 200903053590484247