研究者基本情報

• 博士（感染症学）, 北海道大学, 2022年03月

• https://researchmap.jp/ogata.shohei

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202201015317821598

• 60962751

• 202201015317821598

• 共生微生物
• マダニ

• ライフサイエンス, 分子生物学

• 博士課程, 国際感染症学院

研究活動情報

• 2023年10月, 第9回北海道大学部局横断シンポジウム, 奨励賞
  マダニの化学物質に対する誘引活性と誘引時の行動解析
• 2018年09月, 6th Sapporo Summer Symposium for One Health (SaSSOH), BEST POSTER AWARD
  Analysis of symbionts and parasites for vector-borne disease control

• First molecular evidence of reptile‐associated Borrelia spp. in tortoise bont ticks ( Amblyomma marmoreum ) collected in Zambia
  Yongjin Qiu; Shohei Ogata; Nchimunya Bubala; Innocent Billy Ng'ombwa; Martin C. Simuunza; Edgar Simulundu; Hirofumi Sawa; Ryo Nakao
  Medical and Veterinary Entomology, Wiley, 2026年05月29日
  研究論文（学術雑誌）, Abstract
  
  Reptile‐associated (REP) borreliae represent a distinct monophyletic lineage of spirochetes transmitted by hard ticks. These organisms have been reported in several regions worldwide, including South Africa, but had not been reported in ticks collected in Zambia until now. In this study, we screened for Borrelia spp. in Amblyomma marmoreum ticks collected from Leopard tortoises ( Stigmochelys pardalis ) in Southern Province, Zambia. Of the 87 tick DNA samples analysed, 74 (85.1%) were positive by Borrelia‐ specific real‐time PCR. Subsequent BLASTn and phylogenetic analyses of flaB and 16S rDNA sequences confirmed that the detected Borrelia spp. belong to REP borreliae and are closely related to Borrelia sp. previously reported from South Africa. This study represents the first molecular confirmation of REP borreliae in A. marmoreum ticks collected in Zambia and expands the known distribution of these spirochetes. The findings underscore the need for further investigations on their ecology and epidemiology to better understand the public and animal health relevance and transmission dynamics of REP borreliae in reptile‐associated tick populations.
• Prevalence and factors associated with malaria amongst under-five children in Senga Hill District, Northern Province, Zambia, a community-based cross-sectional study
  Arthur shaba-santu; Lavel Moonga; Shohei Ogata; Kyoko Hayashida; Martin Chitolongo Simuunza
  Malaria Journal, Springer Science and Business Media LLC, 2026年03月31日
  研究論文（学術雑誌）
• Detection of carbapenem resistance among third-generation cephalosporin-resistant Enterobacterales from small-scale poultry farms in peri-urban Lusaka, Zambia
  Situmbeko J. Nasilele; Misheck Shawa; Harvey K. Kamboyi; Bruno S. J. Phiri; Tapiwa Lundu; Mike Nundwe; Angela Lungu; Ladslav Moonga; Shohei Ogata; Masahiro Kajihara; Hirofumi Sawa; Yasuhiko Suzuki; Hideaki Higashi; Ntombi B. Mudenda; Mudenda B. Hang’ombe; Kaampwe Muzandu
  Access Microbiology, 8, 1, Microbiology Society, 2026年01月01日
  研究論文（学術雑誌）, Background. Carbapenem and third-generation cephalosporin (3GC) resistance among Gram-negative bacteria poses a serious threat to human and animal health. This study aimed to identify and characterize carbapenem- and 3GC-resistant Enterobacterales isolated from poultry in Lusaka Province, Zambia.
  
  Methods. Ninety pooled cloacal samples were collected from market-ready broiler chickens in the Chongwe and Chilanga districts of Lusaka Province. The isolates were screened for 3GC and carbapenem resistance using the disc diffusion and broth microdilution methods. PCR and Sanger sequencing were performed for species identification and detection of β-lactamase-encoding ( bla ) genes, including bla _CTX-M , bla _TEM , bla _OXA-1 and bla _SHV . Hierarchical clustering was used to assess phenotypic and genotypic relationships.
  
  Results. A total of 83 3GC-resistant Gram-negative isolates were recovered, of which 12% were also carbapenem resistant. Escherichia coli was the most prevalent species, followed by Klebsiella pneumoniae and Enterobacter spp., then Pseudomonas aeruginosa, other Pseudomonas spp., Acinetobacter baumannii , Citrobacter freundii and Aeromonas caviae . Multidrug resistance occurred in 84.3% of the isolates, with the highest resistance to ampicillin, tetracycline and co-trimoxazole. Overall, 80.7% of the isolates harboured at least one of the four tested bla genes, with bla _CTX-M and bla _TEM being the most common. Hierarchical clustering revealed that isolates from both districts shared similar phenotypic and genotypic resistance patterns.
  
  Conclusions. The presence of multidrug- and carbapenem-resistant Enterobacterales from poultry highlights the emergence of carbapenem resistance in Zambia’s food production sector. The detection of imipenem-resistant isolates indicates the potential for transmission of resistance genes between animals and humans. These findings underscore the need for prudent antimicrobial use, strengthened stewardship and a One Health surveillance approach to contain the spread of carbapenem resistance genes.
• Presence or Emergence of Canine Leishmaniasis, Malawi
  Boniface Chikufenji; Kyoko Hayashida; Yasuyuki Goto; Tatsuki Sugi; Chizu Sanjoba; Chrispin Njala; Inga McDermott; Frederic Lohr; Dagmar Mayer; Shohei Ogata; Masahiro Kajihara; Naganori Nao; Ryo Nakao; Laston Chimaliro; Donales Kapira; Janelisa Musaya; Junya Yamagishi; Elisha Chatanga
  Emerging Infectious Diseases, 32, 1, Centers for Disease Control and Prevention (CDC), 2026年01月
  英語, 研究論文（学術雑誌）
• A high prevalence of dogs seropositive to Leishmania in Zambia
  Herman M. Chambaro; Kyoko Hayashida; Lavel C. Moonga; Misheck Shawa; Walter Muleya; Joseph Chizimu; David Squarre; Tatsuki Sugi; Junya Yamagishi; Shohei Ogata; Masahiro Kajihara; Hirofumi Sawa; Chizu Sanjoba; Enala T. Mwase; Roma Chilengi; Gilbert H. Munsaka; Kelvin L. Sarenje; Namwiinga R. Mulunda; Mable M. Mutengo; Boniface Namangala; Yasuyuki Goto
  Parasitology International, 108, 103081, 103081, Elsevier BV, 2025年10月
  研究論文（学術雑誌）
• In silico characterization of chromosomally integrated blaCTX-M genes among clinical Enterobacteriaceae in Africa: insights from whole-genome analysis
  Misheck Shawa; Herman Chambaro; Harvey K. Kamboyi; Clement Sulwe; Joseph Y. Chizimu; Situmbeko J. Nasilele; Shohei Ogata; Mulemba Samutela; Tuvshinzaya Zorigt; Steward Mudenda; Manyando Simbotwe; Mwamba Nsofwa; Jedidiah Chanda; Freeman Chabala; Mike Nundwe; Joseph Ndebe; Msangwa Sinjani; Kyoko Hayashida; Naganori Nao; Roma Chilengi; Hirofumi Sawa; Yasuhiko Suzuki; Bernard Hang'ombe; Masahiro Kajihara; Hideaki Higashi
  Frontiers in Microbiology, 16, Frontiers Media SA, 2025年09月12日
  研究論文（学術雑誌）, Antimicrobial resistance (AMR) mediated by extended-spectrum β-lactamases (ESBLs) is a growing global concern, particularly among Enterobacteriaceae. The CTX-M-type ESBLs, encoded by the bla_CTX-M gene, are of significant public health importance due to their high prevalence and broad geographic distribution. Typically located on plasmids and often co-occurring with other AMR genes, bla_CTX-M contributes to multidrug resistance (MDR). However, increasing evidence suggests secondary chromosomal integration of bla_CTX-M, sometimes alongside other resistance determinants. The extent and implications of this mechanism remain poorly characterized, especially in Africa, where genomic surveillance is limited. In this study, we retrieved 295 chromosomal sequences of Enterobacteriaceae of African origin from the GenBank and performed in silico predictions of bla_CTX-M and other AMR genes. bla_CTX-M-carrying sequences were further characterized by in silico multilocus sequence typing and genome annotation. Chromosomal insertions were identified through alignment with reference genomes. Overall, 47 of 295 sequences (15.9%) harbored the bla_CTX-M gene, with the highest prevalence in Klebsiella pneumoniae (29/157, 18.5%), followed by Escherichia coli (13/72, 18.1%), Enterobacter spp. (4/38, 10.5%), and Shigella spp. (1/12, 8.3%). The most common allele was bla_CTX-M-15 (31/47, 66.0%), followed by bla_CTX-M-14 (12/47, 25.5%), bla_CTX-M-55 (3/47, 6.4%), and bla_CTX-M-27 (1/27, 3.7%). Co-occurrence of bla_CTX-M with additional AMR genes was frequently observed, with integration events often associated with mobile genetic elements such as ISEcp1 and IS26. Notably, strains from the same hospital setting were phylogenetically related and shared sequence types and AMR gene profiles, suggesting local clonal dissemination. These findings reveal a notable presence of chromosomally integrated bla_CTX-M among African Enterobacteriaceae, frequently in association with other resistance genes, thereby facilitating stable MDR propagation independent of plasmid maintenance. This evolutionary adaptation may have significant implications for the persistence and spread of MDR in clinical settings.
• Development of a PCR-dipstick DNA chromatography-based tool for the detection of CTX-M- and TEM-producing Escherichia coli and Klebsiella pneumoniae isolated from patients in Kafue and Katete districts of Zambia.
  Misheck Shawa; Kyoko Hayashida; Naganori Nao; Atmika Paudel; Harvey Kamboyi; Herman Chambaro; Malala Mulavu; Cynthia Sipho Khumalo; Mike Nundwe; Tuvshinzaya Zorigt; Chinatsu Nakamura; Yongjin Qiu; Naoko Kawai; Maisa Kasanga; Joseph Chizimu; Lavel Moonga; Joseph Ndebe; Manyando Simbotwe; Shohei Ogata; Mulemba Samutela; Chie Nakajima; Roma Chilengi; Mable Mutengo; Masahiro Kajihara; Hirofumi Sawa; Bernard Hang'ombe; Yasuhiko Suzuki; Hideaki Higashi
  BMC infectious diseases, 25, 1, 541, 541, 2025年04月16日, ［国際誌］
  英語, 研究論文（学術雑誌）, In Zambia, 40% of clinical Gram-negative bacteria are either Escherichia coli or Klebsiella pneumoniae, with a high third-generation cephalosporin (3GC) resistance prevalence. Therefore, 3GC resistance surveillance is a crucial indicator for guiding focused intervention policies. However, the lack of genotypic diagnostic tools limits the ability to elucidate trends, especially in peri-urban and rural areas of developing countries. This study aimed to develop a rapid, cost-effective tool for the genotypic surveillance of 3GC resistance. Here, 900 stool samples collected from patients in Kafue (peri-urban, n = 400) and Katete (rural, n = 500) districts of Zambia were used for bacterial isolation on MacConkey agar supplemented with 1 μg/ml cefotaxime. Isolated 3GC-resistant strains were characterized by sequencing the 16S rRNA gene and screening for blaCTX-M and blaTEM genes using single polymerase chain reaction (PCR). Furthermore, selected 3GC-resistant strains were subjected to whole-genome sequencing (WGS) using MiSeq/HiSeq (n = 34) and MinION (n = 1). Using the data from this and other previous studies, we developed a rapid PCR-dipstick DNA chromatography-based tool for detecting blaCTX-M, blaTEM, E. coli-specific yaiO, and K. pneumoniae-specific khe genes. The prevalence of isolated 3GC resistant strains was 15.4% (139/900), dominated by E. coli (102/139, 73.4%). On PCR, the blaCTX-M gene was detected in 72.7% (101/139) of the isolates, while blaTEM was found in 46.8% (65/139) of the strains. The developed tool displayed a high level of agreement with WGS and single PCR/Sanger sequencing, with sensitivity and specificity ≥ 95% and Kappa ≥ 0.95 for each of the four target genes. We envisage that the simplicity and adaptability of this tool will be a significant advantage for the surveillance of 3GC resistance in Zambia and elsewhere.
• Intra-individual polymorphisms in the mitochondrial COI gene of tick-killing Ixodiphagus wasps parasitizing Haemaphysalis flava ticks.
  Yurie Taya; Yuto Shiraki; Samuel Kelava; Naoki Fujisawa; Yuma Ohari; Mackenzie L Kwak; Saori Baba; Hideka Numata; Gita Sadaula Pandey; Yuki Ohsugi; Yuki Katada; Shiho Niwa; Shohei Ogata; Keita Matsuno; Nariaki Nonaka; Ryo Nakao
  Acta tropica, 107510, 107510, 2024年12月16日, ［国際誌］
  英語, 研究論文（学術雑誌）, Ixodid ticks are significant vectors of pathogens affecting both humans and animals. Biological control with natural enemies represents a sustainable tool for managing ticks. However, there is a substantial lack of knowledge about the natural enemies of ticks. Wasps of the genus Ixodiphagus (Encyrtidae) are currently the only known tick-specific parasitoids. While these wasps have been sporadically recorded worldwide, their presence in Eastern Asia is poorly documented. In this study, we investigated the occurrence of Ixodiphagus wasps in field-collected ticks reared on rabbits under laboratory conditions. Ticks were collected from the Hokkaido, Hokuriku, Chugoku, Shikoku, and Kyushu regions in Japan. Out of 1,933 Haemaphysalis ticks attached to rabbits, adult wasps emerged from 15 engorged ticks. All the ticks from which wasps emerged were morphologically and molecularly identified as Haemaphysalis flava. Additionally, wasp DNA was detected in unfed H. flava nymphs using a newly designed Ixodiphagus-specific PCR assay. Among nine experimental sites in the Chugoku region, Ixodiphagus wasps were detected at three sites, with parasitism rates ranging from 1.8% to 8.1%. Finally, the mitochondrial COI genes of four wasp and two tick samples were characterized using shotgun sequencing, direct sequencing and in-fusion cloning approaches. Multiple intra-individual polymorphisms were observed in all the tested samples. Further studies are needed to investigate the relationship between Ixodiphagus wasps and Ixodid ticks. An increased understanding of these parasitoid wasps could contribute to future biological control measures against ticks.
• Experimental demonstration of the transmission of Spiroplasma between different arthropod taxa
  Shohei Ogata; Naoki Hayashi; Yuki Eshita; Yasuha Nagasawa; Nariaki Nonaka; Ryo Nakao
  Journal of Medical Entomology, Oxford University Press (OUP), 2024年02月21日, ［査読有り］, ［筆頭著者］
  研究論文（学術雑誌）, Abstract
  
  Spiroplasma (Mycoplasmatales: Spiroplasmataceae) is one of the most widely distributed symbionts of arthropods. Spiroplasma species can infect their hosts via vertical or horizontal transmission. However, the mode of transmission of Spiroplasma between different arthropod taxa has not been elucidated. In this study, we investigated the potential for the transmission of Spiroplasma to non-native arthropod species, using 2 Spiroplasma spp. isolated from ticks, namely Spiroplasma ixodetis and Spiroplasma mirum, and 3 species of mosquito laboratory colonies, namely Aedes albopictus, Aedes aegypti, and Culex pipiens pallens (Diptera: Culicidae). After feeding the adult mosquitoes with Spiroplasma-containing artificial meals, they were kept at 25 °C for 10 days. Homogenates prepared from Spiroplasma-fed mosquitoes were used to re-isolate Spiroplasma using the in vitro culture method. Nine weeks after culture initiation, the presence of Spiroplasma was tested using the polymerase chain reaction (PCR). The results revealed that only S. ixodetis was detected from all 3 species of mosquitoes and re-isolated from 2 of them. The differences in the infection ability of different Spirolasma species could be attributed to several factors, including environmental effects. Nevertheless, this is the first experimental demonstration of Spiroplasma transmission among different arthropod taxa. Further studies are needed to elucidate the evolutionary mechanism that supports the survival of Spiroplasma in nature.
• Investigation of vertical and horizontal transmission of Spiroplasma in ticks under laboratory conditions
  Shohei Ogata; Rika Umemiya-Shirafuji; Kodai Kusakisako; Keita Kakisaka; Elisha Chatanga; Naoki Hayashi; Yurie Taya; Yuma Ohari; Gita Sadaula Pandey; Abdelbaset Eweda Abdelbaset; Yongjin Qiu; Keita Matsuno; Nariaki Nonaka; Ryo Nakao
  Scientific Reports, 13, 1, Springer Science and Business Media LLC, 2023年08月15日, ［査読有り］, ［筆頭著者］
  研究論文（学術雑誌）, Abstract
  
  Many arthropods harbour bacterial symbionts, which are maintained by vertical and/or horizontal transmission. Spiroplasma is one of the most well-known symbionts of ticks and other arthropods. It is still unclear how Spiroplasma infections have spread in tick populations despite its high prevalence in some tick species. In this study, Ixodes ovatus, which has been reported to harbour Spiroplasma ixodetis at high frequencies, was examined for its vertical transmission potential under experimental conditions. Next, two isolates of tick-derived Spiroplasma, S. ixodetis and Spiroplasma mirum, were experimentally inoculated into Spiroplasma-free Haemaphysalis longicornis colonies and the presence of Spiroplasma in their eggs and larvae was tested. Our experimental data confirmed that S. ixodetis was transmitted to eggs and larvae in a vertical manner in the original host I. ovatus. In the second experiment, there was no significant difference in engorged weight, egg weight, and hatching rate between Spiroplasma-inoculated and control H. longicornis groups. This suggested that Spiroplasma infection does not affect tick reproduction. Spiroplasma DNA was only detected in the eggs and larvae derived from some individuals of S. ixodetis-inoculated groups. This has demonstrated the potential of horizontal transmission between different tick species. These findings may help understand the transmission dynamics of Spiroplasma in nature and its adaptation mechanism to host arthropod species.
• Comparative mitogenomics elucidates the population genetic structure of Amblyomma testudinarium in Japan and a closely related Amblyomma species in Myanmar
  Wessam Mohamed Ahmed Mohamed; Mohamed Abdallah Mohamed Moustafa; May June Thu; Keita Kakisaka; Elisha Chatanga; Shohei Ogata; Naoki Hayashi; Yurie Taya; Yuma Ohari; Doaa Naguib; Yongjin Qiu; Keita Matsuno; Saw Bawm; Lat Lat Htun; Stephen C. Barker; Ken Katakura; Kimihito Ito; Nariaki Nonaka; Ryo Nakao
  Evolutionary Applications., 15, 7, 1062, 1078, Wiley, 2022年06月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• High infection rate of tick-borne protozoan and rickettsial pathogens of cattle in Malawi and the development of a multiplex PCR for Babesia and Theileria species identification
  Elisha Chatanga; Emmanuel Maganga; Wessam Mohamed Ahmed Mohamed; Shohei Ogata; Gita Sadaula Pandey; Abdelbaset; Eweda Abdelbaset; Kyoko Hayashida; Chihiro Sugimoto; Ken Katakura; Nariaki Nonaka; Ryo Nakao
  Acta Tropica, 231, 106413, 106413, 2022年03月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, Malawi has an estimated cattle population of 1,884,803 heads, the indigenous Malawi zebu breed accounts for 91.2%, while the exotic and crossbred accounts for the remaining 8.8%. Although ticks and tick-borne diseases are widespread in Malawi, no molecular study has been conducted to investigate the tick-borne Anaplasmataceae and piroplasms infecting cattle. To provide an insight into the current status of tick-borne pathogens (TBPs) of cattle, a molecular survey was conducted in the central and southern regions of Malawi. A total of 191 cattle of which 132 were Malawi zebu, 44 were Holstein Friesian and 15 were Holstein-Friesian/ Malawi zebu crosses were screened for Anaplasmataceae and piroplasms using the heat shock protein groEL gene and 18S rDNA, respectively. A new 18S rDNA multiplex PCR assay was designed for Babesia and Theileria species identification without sequencing. Overall, 92.3% (n = 177) of the examined animals were infected with at least one TBP. Anaplasmataceae-positive rate was 57.6% (n = 110) while for piroplasms it was 80.1% (n = 153). The detected Anaplasmataceae were Anaplasma bovis 2.6% (n = 5), Anaplasma marginale 24.6% (n = 47), Anaplasma platys-like 13.6% (n = 26), uncharacterized Anaplasma sp. 14.1% (n = 27), and uncharacterized Ehrlichia sp. 16.2% (n = 31). The detected piroplasms were Babesia bigemina 2.6% (n = 5), Theileria mutans 73.8% (n = 141), Theileria parva 33.0% (n = 63), Theileria taurotragi 12.6% (n = 24), and Theileria velifera 53.4% (n = 102). Mixed infection rate was found in 79.6% (n = 152) of the samples analyzed. This study has shown a high burden of TBPs among cattle in Malawi which highlights the need to conceive new methods to control ticks and TBPs in order to improve animal health and productivity. The newly developed multiplex PCR assay would be a useful tool especially in resource limited settings where sequencing is not available and when mixed infections are expected.
• Molecular survey of Babesia and Anaplasma infection in cattle in Bolivia.
  Shohei Ogata; Juan Antonio Cristian Pereira; Loza Vega Ariel Jhonny; Herbas Perez Gladys Carolina; Keita Matsuno; Yasuko Orba; Hirofumi Sawa; Fumihiko Kawamori; Nariaki Nonaka; Ryo Nakao
  Veterinary Sciences, 8, 9, 188, 188, MDPI AG, 2021年09月07日, ［査読有り］, ［筆頭著者］, ［国際誌］
  英語, 研究論文（学術雑誌）, Latin American countries produce more than a quarter of the world’s beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-borne pathogens in cattle from Santa Cruz, Bolivia, where no detailed epidemiological data are available. Blood samples were collected from 104 cattle. Apicomplexan parasites were detected by nested PCR amplification of the 18S ribosomal RNA gene (rDNA), and Anaplasmataceae was screened by the PCR amplification of 16S rDNA, followed by characterisation based on the heat shock protein and citrate synthase gene sequences. Babesia infection was observed in nine cattle (one Babesia bovis and eight Babesia bigemina), while Anaplasmataceae infection was detected in thirty-two cattle. A sequencing analysis confirmed the presence of Anaplasma marginale and Anaplasma platys-like. These results provide the first molecular evidence for the four above-mentioned tick-borne pathogens in cattle in Bolivia. This information improves our understanding of the epidemiology of TBDs and will help in formulating appropriate and improved pathogen control strategies.
• Applications of blocker nucleic acids and non-metazoan PCR improves the discovery of the eukaryotic microbiome in ticks.
  Yurie Taya; Gohta Kinoshita; Wessam Mohamed Ahmed Mohamed; Mohamed Abdallah Mohamed Moustafa; Shohei Ogata; Elisha Chatanga; Yuma Ohari; Kodai Kusakisako; Keita Matsuno; Nariaki Nonaka; Ryo Nakao
  Microorganisms, 9, 5, 2021年05月13日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Spiroplasma infection among ixodid ticks exhibits species dependence and suggests a vertical pattern of transmission.
  Shohei Ogata; Wessam Mohamed Ahmed Mohamed; Kodai Kusakisako; May June Thu; Yongjin Qiu; Mohamed Abdallah Mohamed Moustafa; Keita Matsuno; Ken Katakura; Nariaki Nonaka; Ryo Nakao
  Microorganisms, 9, 2, 2021年02月08日, ［査読有り］, ［筆頭著者］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Amblyomma testudinarium infestation on a brown bear (Ursus arctos yesoensis) captured in Hokkaido, a northern island of Japan.
  Ryo Nakao; Kohei Shinjo; Tomoki Sakiyama; Shohei Ogata; Kodai Kusakisako; Gohta Kinoshitaa; Doaa Naguiba; Elisha Chatanga; Wessam Mohamed Ahmed Mohamed; Mohamed Abdallah Mohamed Moustafa; Keita Matsuno; Takuya Itoi; Nariaki Nonaka; Mariko Sashika; Toshio Tsubota; Michito Shimozuru
  Parasitology International, 80, 2021年02月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）
• Complete genome sequence of Rickettsia asiatica strain Maytaro1284, a member of spotted fever group rickettsiae isolated from an Ixodes ovatus tick in Japan.
  May June Thu; Yongjin Qiu; Junya Yamagishi; Kodai Kusakisako; Shohei Ogata; Mohamed Abdallah Mohamed Moustafa; Norikazu Isoda; Chihiro Sugimoto; Ken Katakura; Nariaki Nonaka; Ryo Nakao
  American Society for Microbiology, 8, 37, American Society for Microbiology, 2019年09月12日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, This is the first report of the complete genome sequence of
  Rickettsia asiatica
  strain Maytaro1284, isolated from an
  Ixodes ovatus
  tick in Japan. The genome contains a 1,344,324-bp circular chromosome and one plasmid of 74,761 bp. There was no outer membrane protein A (
  ompA
  ) gene encoded in the genome.
• Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases
  Yasuko Sekita; Keiji Murakami; Hiromichi Yumoto; Takashi Amoh; Natsumi Fujiwara; Shohei Ogata; Takashi Matsuo; Yoichiro Miyake; Yoshiki Kashiwada
  BioMed Research International, 2016, 2016年06月20日, ［査読有り］
  英語

• 北海道斜里町においてエゾヒグマより採集されたタカサゴキララマダニの一例
  下鶴 倫人; 新庄 康平; 崎山 智樹; 小方 昌平; 草木迫 浩大; 木下 豪太; Mohamed Wessam Mohamed Ahmed; Moustafa Mohamed Abdallah Mohamed; 松野 啓太; 伊東 拓也; 野中 成晃; 佐鹿 万里子; 坪田 敏男; 中尾 亮, 日本獣医学会学術集会講演要旨集, 163回, 327, 327, 2020年10月
  (公社)日本獣医学会, 日本語

• 温水処理がマダニの生存率に与える影響と制御技術としての可能性
  小方昌平; 小山由美; 渡部浩平
  第33回ダニと疾患のインターフェイスに関するセミナー, 2026年06月05日, 日本語, 口頭発表（一般）
  2026年06月05日 - 2026年06月07日
• アフリカにおける狂犬病サーベイランス強化のための 迅速診断法とゲノム手法の導入：ケニアおよびザンビアでの取り組み
  齊藤信夫; 君付和範; 小方昌平
  第66回日本熱帯医学会大会, 2025年11月29日
• スピロプラズマのマダニ集団内における伝播様式に関する研究
  小方 昌平; 白藤 梨可; 草木迫 浩大; 柿坂 圭太; Elisha Chatanga; 林 直樹; 田谷 友里恵; 尾針 由真; Gita Sadaula Pandey; Abdelbaset Eweda Abdelbase; 邱 永晋; 松野 啓太; 野中 成晃; 中尾 亮; Shohei Ogata; Rika Umemiya-Shirafuji; Kodai Kusakisako; Keita Kakisaka; Elisha Chatanga; Naoki Hayashi; Yurie Taya; Yuma Ohari; Gita Sadaula Pandey; Abdelbaset Eweda Abdelbase; Yongjin Qiu; Keita Matsuno; Nariaki Nonaka; Ryo Nakao
  日本マイコプラズマ学会 第52回学術集会, 2025年05月23日, 口頭発表（招待・特別）
  2025年05月23日 - 2025年05月24日, ［招待講演］
• Understanding the vertical and horizontal transmission potential of Spiroplasma in ticks
  Shohei Ogata; Rika Umemiya-Shirafuji; Kodai Kusakisako; Keita Kakisaka; Elisha Chatanga; Naoki Hayashi; Yurie Taya; Yuma Ohari; Gita Sadaula Pandey; Abdelbaset Eweda Abdelbase; Yongjin Qiu; Keita Matsuno; Nariaki Nonaka; Ryo Nakao
  11th Tick & Tick-borne Pathogen Conference, 2024年09月02日, 英語, ポスター発表
  2024年09月01日 - 2024年09月06日, キューバ共和国, ［国際会議］
• Zoonosis surveillance in Zambia and academic contribution to foster zoonosis experts under the One Health concept.
  3rd International Conference on Public Health in Africa (CPHIA 2023), 2023年11月, シンポジウム・ワークショップパネル（公募）
  2023年11月27日 - 2023年11月30日
• マダニ × ファッション - 従来のマダニ感染症の啓蒙活動に対する問題へのファッションを取り入れた試み -
  小方昌平; 西晃平
  超異分野学会 東京大会2023, 2023年03月04日, 日本語, ポスター発表
  2023年03月03日 - 2023年03月04日
• ボリビア多民族国サンタクルス県の牛におけるBabesiaおよびAnaplasmaの感染状況
  小方昌平、Juan Antonio Pereira、松野啓太、大場靖子、澤洋文、川森文彦、野中成晃、中尾亮
  第164回日本獣医学会学術集会, 2021年09月, 日本語, 口頭発表（一般）
  2021年09月07日 - 2021年09月13日, ［国内会議］
• 人への病原性が示唆されるマダニ共生細菌の検出および⼆酸化炭素誘引法による⽇本産マダニ採集への挑戦
  小方昌平、Wessam Mohamed Ahmed Mohamed、草木迫浩大、May June Thu、邱永晋、Mohamed Abdallah Mohamed Moustafa、松野啓太、片倉賢、野中成晃、中尾亮
  第２８回ダニと疾患のインターフェイスに関するセミナー, 2021年09月, 日本語
  ［国内会議］
• Molecular analysis of Babesia and Anaplasma in cattle in Bolivia.
  Shohei Ogata; Loza Vega Ariel Jhonny; Juan Antonio C. Pereira; Keita Matsuno; Nariaki Nonaka; Ryo Nakao
  The 8th Sapporo Summer Symposium for One Health 2020, 2020年09月, 英語, 口頭発表（一般）
  2020年09月16日 - 2020年09月17日, ［国際会議］
• The diversity of Spiroplasma sp. in ticks.
  Shohei Ogata; Ryo Nakao; Nariaki Nonaka
  The 7th Sapporo Summer Symposium for One Health 2019, 2019年09月, 英語, ポスター発表
  2019年09月19日 - 2019年09月20日
• マダニが保有するスピロプラズマ属細菌の多様性
  小方昌平、中尾亮、野中成晃、片倉賢
  第162回日本獣医学会学術集会, 2019年09月, 日本語, 口頭発表（一般）
  2019年09月10日 - 2019年09月12日, ［国内会議］
• Analysis of symbionts and parasites for vector-borne disease control.
  Shohei Ogata; Yuki Eshita; Ryo Nakao
  The 6th Sapporo Summer Symposium for One Health 2018, 2018年09月, 英語, ポスター発表
  2018年09月20日 - 2018年09月21日

• ザンビアにおける狂犬病ウイルススピルオーバーと感染伝播解明に関する研究
  新興・再興感染症研究基盤創生事業（海外拠点活用研究領域）
  2024年12月 - 2027年03月
  齊藤信夫; 君付和範; 小方昌平
  日本医療研究開発機構 AMED, 研究分担者, 24wm0225043h0001
• 温水除草技術を応用した薬剤非依存性マダニ防除手法の開発とダニ媒介感染症予防戦略
  科学研究費助成事業
  2023年04月01日 - 2026年03月31日
  小方 昌平
  植生上から採取し、形態学的に鑑別を行ったフタトゲチマダニの若虫および成虫合わせて約700個体に対して温度依存性の生存試験を行った。本実験の温度範囲を決めるため、45℃/120秒、 50℃/5秒、55℃/5秒で若ダニ、成ダニそれぞれに対して温水浸漬実験を行い、本実験の温度範囲を決定した。マダニの各ライフステージを45℃、50℃、55℃、57℃、60℃、62℃、65℃に設定した恒温水に1秒間浸漬し、生存数を評価した。サンプル数は10～200とし、浸漬後のマダニを25℃で7日間飼育し、浸漬日、1日後、7日後の生存数を記録し比較した。フタトゲチマダニの若虫において、45℃/120秒と50℃/1秒の温水に浸漬したマダニの7日後生存率は100％であった。また、50℃/5秒で温水浸漬したマダニの7日後生存率は88％（44/50匹）であった。55℃/1秒の温水に浸漬したマダニの7日後生存率は97％(97/100匹)、57℃/1秒では98％(49/50匹)、60℃/1秒の温水では、6％(12/200匹)であった。62℃/1秒以上の温水では、すべてのマダニが死亡した。フタトゲチマダニの成虫において、45℃/120秒、 50℃/5秒、55℃/5秒の温水に浸漬したマダニの7日後生存率は100％であった。57℃/1秒の温水に浸漬したマダニの7日後生存率は、メスマダニ94％(15/16匹)、オスマダニ87％(13/15匹)、60℃/1秒の温水では、メスマダニ46％(16/35匹)、オスマダニ33％(7/21匹)であった。62℃/1秒以上の温水では、すべての成虫ダニが死亡した。温水浸漬直後、1日後および7日後に調査したマダニの生存率に有意差は見られなかった。本研究では国内のフタトゲチマダニの温水耐性に関する初めての研究を実施し、62℃の温水に１秒以上浸漬することで全てのマダニが死亡することが確認できた。
  日本学術振興会, 若手研究, 北海道大学, 23K14094
• マダニ媒介性感染症研究のボトルネック解消に向けたマダニ実験基盤の国際共同構築
  科学研究費助成事業
  2020年10月27日 - 2024年03月31日
  中尾 亮; 佐々木 東; 邱 永晋; 小方 昌平
  マダニ媒介性感染症の制御に向けて多様な側面から基礎・応用研究が行われているが、マダニ細胞、実験室マダニ飼育系、実験室マダニ維持株はその重要な実験基盤である。本研究では国際共同研究により、日本国内に生息するマダニ種に関するそれらの実験基盤を作出した。従来のマダニ細胞樹立法を改良し、日本国内に優占するチマダニ属マダニの細胞作出に成功した。さらにチマダニ属マダニを含む複数の国産マダニの実験室維持株を樹立した。
  日本学術振興会, 国際共同研究加速基金(国際共同研究強化(B)), 北海道大学, 20KK0151