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渡邉 可菜実 (ワタナベ カナミ)
| 獣医学研究院 | 特任助教 |
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- 61036901
研究キーワード研究分野
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■ 論文- Elephant alcohol dehydrogenase and aldehyde dehydrogenase: Genomic, transcriptomic, and enzymatic analyses raise questions about anecdotal inebriation
Ayuko Morita; Kanami Watanabe; Mitsuki Kondo; Kraisiri Khidkhan; Aksorn Saengtienchai; Chaleamchat Somgird; Patcharapa Towiboon; Taweepoke Angkawanish; Warangkhana Langkaphin; Kittikul Namwongprom; Narueporn Kittisirik; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 308, 110604, 110604, Elsevier BV, 2026年10月, ［筆頭著者］
研究論文（学術雑誌） - Elephant feces as a non-invasive bioindicator of heavy metals and pesticides in the Lower Zambezi National Park, Zambia
Kanami WATANABE; Nelly BANDA; Yared B. YOHANNES; Rio DOYA; John YABE; Kaampwe MUZANDU; Andrew KATABA; Yoshinori IKENAKA; Shouta M.M. NAKAYAMA; Mayumi ISHIZUKA
Environmental Monitoring and Contaminants Research, 6, 63, 74, 2026年05月, ［筆頭著者］
英語, 研究論文（学術雑誌） - Reductive dominance and limited cytochrome P450 activity in neonicotinoid metabolism by elephant liver microsomes.
Kanami Watanabe; Kraisiri Khidkhan; Aksorn Saengtienchai; Chaleamchat Somgrid; Patcharapa Towiboon; Taweepoke Angkawanish; Warangkhana Langkaphin; Kittikul Namwongprom; Narueporn Kittisirik; Yared B Yohannes; Yoshinori Ikenaka; Shouta Mm Nakayama; Mayumi Ishizuka
The Journal of veterinary medical science, 88, 4, 607, 613, 2026年04月02日, ［筆頭著者］, ［国内誌］
英語, 研究論文（学術雑誌）, Neonicotinoids (NNs) are commonly used insecticides; however, their biotransformation in wild mammals remains poorly understood. Asian elephants, which are declining in numbers, may be vulnerable to these chemicals. In this study, we examined the liver metabolism of four major NNs: acetamiprid, imidacloprid, thiamethoxam, and clothianidin, using elephant liver microsomes. Metabolite profiling and targeted analysis were conducted with liquid chromatography quadrupole time-of-flight mass spectrometry and liquid chromatography tandem mass spectrometer, respectively. Results indicated that elephants mainly metabolize these NNs via reductive pathways, suggesting the involvement of nitroreductase-like enzymes. Conversely, cytochrome P450-mediated oxidation may be limited; however, the roles of other enzymatic or microbial systems cannot be ruled out. These findings provide insights into xenobiotic metabolism in elephants, helping evaluate their toxicological vulnerability and supporting conservation efforts and risk assessments. - Intergenerational sex-specific transcriptomic alterations in gonads of mice after parental lead exposure.
Nelly Banda; Kanami Watanabe; John Yabe; Rio Doya; Yared Beyene Yohannes; Yoshinori Ikenaka; Mayumi Ishizuka; Shouta M M Nakayama
Reproductive toxicology (Elmsford, N.Y.), 140, 109154, 109154, 2026年03月, ［国際誌］
英語, 研究論文（学術雑誌）, Lead (Pb) exposure is known to influence gene expression across multiple tissues, potentially affecting germline biology and offspring development. This study investigated transcriptomic alterations in the gonads of F1 mice following four weeks of parental exposure to Pb‑contaminated soil. After exposure, breeding generated four experimental groups: ① exposed dams with unexposed sires, ② exposed sires with unexposed dams, ③both parents exposed, and ④ unexposed controls. Testes and ovaries were collected from 9‑week‑old offspring, and RNA sequencing was performed using Qiagen CLC Genomics Workbench to characterise transcriptional changes. Principal component analysis revealed distinct transcriptomic profiles in Groups 1-3 relative to controls, indicating that parental Pb exposure, whether maternal, paternal, or combined, shaped offspring gonadal gene expression. Heatmap clustering showed partial overlap between Male Group 3 and controls, although unique expression features remained evident. All exposed groups exhibited reduced overall transcript abundance when compared to controls. Males with maternal Pb exposure displayed the highest number of differentially expressed genes (DEGs), with gene ontology analysis highlighting alterations in immune‑related and enzymatic pathways. In contrast, males with both maternal and paternal exposure showed the fewest DEGs, though a greater proportion were located on the Y chromosome. These sex‑specific transcriptional patterns, particularly those observed in the offspring of exposed sires, suggest that Pb‑related molecular effects may arise independently of direct maternal transfer. While the underlying mechanisms remain uncertain, the findings raise the possibility that some effects could be transmitted through heritable pathways. - Integrated genomic, transcriptomic, and microsomal metabolic profiling of Cytochrome P450s in squirrels
Miku GOTO; Kanami WATANABE; Yared Beyene YOHANNES; Nanako HASEGAWA; Ayuko MORITA; Nelly BANDA; Keita KAKISAKA; Masaki ITO; Yoshinori IKENAKA; Shouta M.M. NAKAYAMA; Mayumi ISHIZUKA
Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 2026年
研究論文（学術雑誌） - Anthropogenic Activities Elevate Heavy Metals Levels in Soil and Tree Bark; A Case Study of Lower Zambezi and Mosi-Oa-Tunya National Parks.
Nelly Banda; Kanami Watanabe; Rio Doya; Nyein Chan Soe; Andrew Kataba; John Yabe; Golden Zyambo; Kaampwe Muzandu; Yared Beyene Yohannes; Yoshinori Ikenaka; Mayumi Ishizuka; Shouta M M Nakayama
Archives of environmental contamination and toxicology, 89, 2, 180, 194, 2025年08月, ［国際誌］
英語, 研究論文（学術雑誌）, The impact of various human activities on protected environments remains a significant concern. Mining for ores is crucial in many aspects of society. To achieve global decarbonisation, more metals are required, which will lead to increased worldwide metal exploration and mining. Zambia is primarily a mining country and has expanded exploration into protected lands (national parks). The effects of this exploration on these protected areas is ongoing. This study aimed to establish baseline data for monitoring metal pollution in the Lower Zambezi National Park before mining commenced. It also evaluated whether tourism influenced metal pollution in national parks and how these metals and metalloids correlate within tree bark and soils. To accomplish this, soil and Acacia tree bark samples were collected from Lower Zambezi National Park and Mosi-Oa-Tunya National Park. Arsenic (As), cadmium (Cd), chromium (Cr), cobalt (Co), copper (Cu), mercury (Hg), manganese (Mn), nickel (Ni), lead (Pb), and zinc (Zn) were analysed in this study. The concentrations of metals and metalloids were higher in areas with vehicular traffic and mining activity in the national parks, which may directly impact the biosystems in those areas. Maximum levels of metals in soil in these areas were as follows: As 40.8 mg/kg, Cd 0.879 mg/kg, Cr 241 mg/kg, Hg 1.1 mg/kg, Pb 211 mg/kg, and Zn 1285 mg/kg. When compared to areas of lower anthropogenic activities, differences in metal concentrations were up to 1000-fold. These changes can potentially affect the adaptation of wildlife to various stressors, especially as we are facing global climate change. - A Comparative Genomic and Phylogenetic Investigation of the Xenobiotic Metabolism Enzymes of Cytochrome P450 in Elephants Shows Loss in CYP2E and CYP4A.
Kanami Watanabe; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M M Nakayama; Mayumi Ishizuka
Animals : an open access journal from MDPI, 13, 12, 2023年06月09日, ［筆頭著者］, ［国際誌］
英語, 研究論文（学術雑誌）, Cytochrome P450 is an important enzyme that metabolizes a variety of chemicals, including exogenous substances, such as drugs and environmental chemicals, and endogenous substances, such as steroids, fatty acids, and cholesterol. Some CYPs show interspecific differences in terms of genetic variation. As little is known about the mechanisms of elephant metabolism, we carried out a comparative genomic and phylogenetic analysis of CYP in elephants. Our results suggest that elephant CYP genes have undergone independent duplication, particularly in the CYP2A, CYP2C, and CYP3A genes, a unique cluster specific to elephant species. However, while CYP2E and CYP4A were conserved in other Afrotheria taxa, their decay in elephants resulted in genetic dysfunction (pseudogene). These findings outline several remarkable characteristics of elephant CYP1-4 genes and provide new insights into elephant xenobiotic metabolism. Further functional investigations are necessary to characterize elephant CYP, including expression patterns and interactions with drugs and sensitivities to other chemicals. - Development of a sandwich enzyme-linked immunosorbent assay (ELISA) to quantify γ-glutamyl-carboxylated clotting factor IX and assess redox susceptibility of anticoagulant chemicals.
Ryo Sato; Kanami Watanabe; Ryo Kamata; Kazuki Takeda
The Journal of veterinary medical science, 84, 6, 804, 808, 2022年06月10日, ［筆頭著者］, ［国内誌］
英語, 研究論文（学術雑誌）, Anticoagulant chemicals (ACCs) such as warfarin are widely used in medical applications as well as for their rodenticide properties. Their efficacy is greatly influenced by polymorphisms in the gene encoding vitamin K epoxide reductase (VKOR). Evaluation of the activity of ACCs toward VKOR variants is essential to determine their proper use. Presently, this is achieved by co-expressing VKOR of Rattus Norvegicus and human clotting factor IX in cultured cells and measuring inhibition of vitamin K-dependent gamma-glutamyl carboxylation of factor IX (glaFIX) activity. However, glaFIX has only been quantified using indirect methods like blood coagulation assays. We have developed a sandwich enzyme-linked immunosorbent assay using a glaFIX-specific antibody to quantify glaFIX and used this to analyze inhibition of VKOR activity by warfarin.
- Comparative In Silico and In Vitro Transcriptomic Analysis of Xenobiotic Metabolizing Enzymes in Elephants
Kanami Watanabe; Ayuko Morita; Aksorn Saengtienchai; Kraisiri Khidkhan; Supaphen Sripiboon; Kornsorn Srikulnath; Chatchote Thitaram; Chaleamchat Somgird; Taweepoke Angkawanish; Warangkhana Langkaphin; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
9th Chemical Hazard Symposium, Ehime, Japan, 英語, 口頭発表（一般）
2025年10月23日 - 2025年10月24日 - Comprehensive Study of Heavy Metal and Pesticide Contamination in Fecal Matter of African Savanna Elephants in Lower Zambezi National Park, Zambia
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
International Symposium on Chemical and Biological Hazards on One Health: Towards Sustainable Mineral Development and Pollution Monitoring（ZAZINAMBO Project）. University of Botswana, Gaborone, Botswana, 英語, 口頭発表（一般）
2025年10月08日 - 2025年10月09日 - Tracing Environmental Contaminants via Elephant Feces: A Soil–Plant–Feces Comparison in Lower Zambezi National Park
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
JSPS International Symposium on the Impact of Chemical and Biological Hazards on the Environment, Animals and Human Health and its Countermeasures and JICA ZAZINAMBO Symposium on Establishment of Sustainable Mineral Development Through Strengthening of Monitoring Systems and Human Capacity Toward Heavy Metal Pollution. University of Zimbabwe, Innovation Hub Auditorium, Harare, 英語, 口頭発表（一般）
2025年10月07日 - 2025年10月08日 - Comparative In Silico and In Vitro Transcriptomic Analysis of Phase II Xenobiotic Metabolizing Enzymes in Elephants
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
SETAC 9th World Congress. Johannesburg, South Africa, Sandton Convention Centre, 英語, 口頭発表（一般）
2025年09月29日 - 2025年10月03日 - Tracing Heavy Metal and Pesticide Contamination in African Elephants: A Case Study from Lower Zambezi National Park
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
SETAC Latin America 16th Biennial Meeting, 英語, 口頭発表（一般）
2025年08月25日 - 2025年08月29日 - Comparative In Silico and In Vitro Transcriptomic Analysis of Phase II Xenobiotic Metabolizing Enzymes in Elephants
Kanami Watanabe; Ayuko Morita; Aksorn Saengtienchai; Kraisiri Khidkhan; Supaphen Sripiboon; Kornsorn Srikulnath; Chatchote Thitaram; Chaleamchat Somgird; Taweepoke Angkawanish; Warangkhana Langkaphin; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
9th One Health Lecture Series、Thamassat University, Thailand, 英語, 口頭発表（一般）
2024年12月16日 - 2024年12月17日 - ゾウにおける第II相異物代謝酵素のIn silico遺伝子解析及びIn vitroトランスクリプトーム解析
渡邉可菜実; 森田鮎子; Aksorn Saengtienchai; Kraisiri Khidkhan; Supaphen Sripiboon; Kornsorn Srikulnath,Chatchote Thitaram,Chaleamchat Somgid,Taweepoke Angkawanish; Warangkhana Langkaphin; 近藤充希; 池中良徳; 中山翔太; 石塚真由美
第30回日本野生動物医学会大会、沖縄科学技術大学院大学（OIST）, 日本語, 口頭発表（一般）
2024年12月13日 - 2024年12月15日 - Genetics studies of xenobiotic metabolic enzymes UDP-glucuronosyltransferase(UGT) and Sulfotransferases (SULT) in Elephants reveals unique feature
Kanami Watanabe; Ayuko Morita; Aksorn Saengtienchai; Kraisiri Khidkhan; Supaphen Sripiboon; Kornsorn Srikulnath; Chatchote Thitaram; Chaleamchat Somgird; Taweepoke Angkawanish; Warangkhana Langkaphin; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
SETAC North America 45th Annual Meeting. Fort Worth, Texas Fort Worth Convention Center, 英語, ポスター発表
2024年10月20日 - 2024年10月24日 - In-depth Analysis of Heavy Metal and Pesticide Presence in Fecal Samples from African Savanna Elephants of Lower Zambezi National Park, Zambia
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuk
SETAC North America 45th Annual Meeting. Fort Worth, T exas Fort Worth Convention Center, 英語, ポスター発表
2024年10月20日 - 2024年10月24日 - Assessing the Impact of Human-Induced Environmental Toxins and Pharmacokinetics in Endangered Asian Elephants: Implications for Conservation and Medical Treatment
Kanami Watanabe; Ayuko Morita; Amnart Poapolathep; Saranya Poapolathep; Aksorn Saengtienchai; Kraisiri Khidkhan; Supaphen Sripiboon; Kornsorn Srikulnath; Chatchote Thitaram; Chaleamchat Somgird; Taweepoke Angkawanish; Warangkhana Langkaphin; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
International Symposium on Chemical and Biological Hazards on One Health: Towards Sustainable Mineral Development and Pollution Monitoring（ZAZINAMBO Project）. University of Botswana, Gaborone, Botswana, 英語, ポスター発表
2024年10月08日 - 2024年10月09日 - Heavy Metal and Pesticide Presence in Fecal Samples from African Savanna Elephants in Lower Zambezi National Park, Zambia.
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
21th The International Symposium on Toxicity Assessment. Conference in Fukuoka, Japan, 英語, 口頭発表（一般）
2024年08月25日 - 2024年08月30日 - Comprehensive Assessment of Heavy Metal and Pesticide Contaminants in Fecal Samples from African Savanna Elephants of Lower Zambezi National Park, Zambia
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
7th International Chemical Hazard Symposium, Ehime, Japan, 英語, 口頭発表（一般）
2023年12月13日 - 2023年12月14日 - Simultaneous analysis of heavy metal and pesticides in feces of wild African savanna elephants in Lower Zambezi National Park, Zambia
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
The 19th International Elephant Conservation and Research Symposium. Chiang Mai, Thailand, 英語, 口頭発表（一般）
2023年11月14日 - 2023年11月17日 - Assessing Heavy Metal and Pesticide Contamination of Feces from Wild African Savanna Elephants in Lower Zambezi National Park, Zambia
Kanami Watanabe; Nelly Banda; Yared B. Yohannes; Rio Doya; John Yabe; Kaampwe Muzandu; Andrew Kataba; Yoshinori Ikenaka; Shouta; M.M. Nakayama; Mayumi Ishizuka
-International Symposium- Impact of Chemical and Biological Hazards on the Environment, Animals and Human Health and its Countermeasure. Alte Brucke Holiday Resort and Conference Center, Swakopmund, Namibia, 英語, 口頭発表（一般）
2023年10月10日 - 2023年10月11日 - Genetics studies of xenobiotic metabolic enzymes ChytocromeP450 (CYP) and UDP-glucuronosyltransferase in Elephant
Kanami Watanabe; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
1st AJ-CORE/ToRePs Symposium、Hokkaido University (Hybrid), 2023年02月08日, 英語, 口頭発表（一般） - Comparative genomic and phylogenetic investigation of the xenobiotic metabolism enzymes in Elephant
Kanami Watanabe; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
6th International Chemical Hazard Symposium ＆ 6th Symposium of Japan Society for Environmental Chemistry, 英語, 口頭発表（一般）
2023年01月12日 - 2023年01月13日 - Phylogenetic analysis of Elephant Cytochrome P450 (CYP) gene family
Kanami Watanabe; Mitsuki Kondo; Yoshinori Ikenaka; Shouta M.M. Nakayama; Mayumi Ishizuka
5th International Conference of Asian Society of Conservation Medicine, Chiang Mai University, Thailand., 英語, 口頭発表（一般）
2022年10月25日 - 2022年10月28日 - ゾウにおけるシトクロームP450(CYP)ファミリーの系統解析
渡邉可菜実; 近藤充希; 池中良徳; 中山翔太; 石塚真由美
第 28 回日本野生動物医学会大会、 筑波、茨城, 日本語, 口頭発表（一般）
2022年09月22日 - 2022年09月24日 - 培養細胞法とAlphaFold2 による血液凝固第IX因子スプライシングバリアント2の機能解析
渡邉 可菜実; 佐藤玲; 鎌田亮; 武田 一貴
第49回日本毒性学会学術年会, 札幌, 北海道, 2022年06月30日, 日本語, 口頭発表（一般） - 血液凝固第 IX 因子スプライシングバリアント 2 の機能解析
渡邉 可菜実; 佐藤玲; 鎌田亮; 武田 一貴
第4回日本比較薬理学毒性学会春季研究会, 2022年02月05日, 日本語, 口頭発表（一般） - Characterization of Clotting Factor IX Transcript variant 2
Kanami Watanabe; Ryo Sato; Ryo Kamata; Kazuki Takeda
5th International Chemical Hazard Symposium, 日本語, 口頭発表（一般）
- 希少動物ゾウの保全に向けた環境化学物質曝露実態と異物代謝機構の解明
科学研究費助成事業
2026年07月31日 - 2028年03月31日
渡邉 可菜実
日本学術振興会, 研究活動スタート支援, 北海道大学, 26K25603 - 希少動物保全に向けたゾウとその腸内細菌叢が持つ異物代謝機能の解明
科学研究費助成事業
2024年04月23日 - 2026年03月31日
渡邉 可菜実
本研究ではゾウの代謝機構に関する理解を深めるために、二つの観点に着目して調査を進めている。第一に、未解明の異物代謝酵素およびその機能の解明を目指し、ゾウに特異的な酵素群の発現と活性を明らかにすることで、新たな代謝経路の存在を検討している。第二に、腸内細菌叢が異物代謝に果たす役割に着目し、宿主の代謝機構と腸内微生物叢の相互作用を含めた包括的な代謝システムの理解を試みている。
2023年度からは、タイの研究機関と連携し、死亡したゾウの個体から肝臓、腎臓、腸管などの新鮮な臓器を収集する体制を確立した。これらの臓器サンプルを用いてRNAの抽出および酵素活性測定を実施し、代謝酵素の発現解析と代謝活性試験を進めている。その結果、これまでin silico解析により予測されていた仮説を実証する新たなデータが得られつつあり、ゾウの代謝機構に関する理解が着実に進展している。
また、ザンビア・ローワーザンベジ国立公園において2022年度から2023年度にかけて採取したアフリカゾウの糞便サンプルを用いた研究では、環境中の化学物質への曝露評価を目的に、農薬（殺虫剤・除草剤）および重金属の濃度分析を行った。分析にはGC-MS/MS、LC-MS/MS、LC-QTOF/MS、ICP-MSといった質量分析機器を用いた。加えて、同地域の植物および土壌サンプルにも同様の分析を行った結果、植物と糞便の両方から共通の農薬類が検出され、同地域におけるゾウが植物を介して農薬に曝露している可能性が示唆された。さらに、一部の重金属に関しては、採取年度や地点によって濃度に有意な差が見られ、人為的影響の強い地域において高濃度を示す傾向が明らかとなった。
現在は、これらの化学物質がゾウの体内でどのように蓄積され、どのように排泄されるか、さらにそれらがゾウの健康に及ぼす影響について、より詳細な検討を進めている。
日本学術振興会, 特別研究員奨励費, 北海道大学, 24KJ0285
