研究者基本情報

• https://researchmap.jp/taniguchi_atsushi

• https://orcid.org/0000-0001-5500-0671
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202001004530876308

• https://orcid.org/0000-0001-5500-0671

• 202001004530876308

• ライフサイエンス, 細胞生物学

研究活動情報

• Statistical and mechanical analysis of multi-pseudopodial locomotion in a testate amoeba, Arcella sp.
  Genta MATSUMOTO; Atsushi TANIGUCHI; Mami NOMURA; Satoshi SHIMANO; Jean-Paul RIEU; Katsuhiko SATO; Toshiyuki NAKAGAKI; Yukinori NISHIGAMI
  Proceedings of the Japan Academy, Series B, 102, 1, 57, 70, Japan Academy, 2026年01月09日
  研究論文（学術雑誌）
• Discovery of essential kinetoplastid-insect adhesion proteins and their function in Leishmania-sand fly interactions
  Ryuji Yanase; Katerina Pruzinova; Barrack O. Owino; Edward Rea; Flávia Moreira-Leite; Atsushi Taniguchi; Shigenori Nonaka; Jovana Sádlová; Barbora Vojtkova; Petr Volf; Jack D. Sunter
  Nature Communications, 15, 1, Springer Science and Business Media LLC, 2024年08月13日, ［査読有り］
  研究論文（学術雑誌）, Abstract
  
  Leishmania species, members of the kinetoplastid parasites, cause leishmaniasis, a neglected tropical disease, in millions of people worldwide. Leishmania has a complex life cycle with multiple developmental forms, as it cycles between a sand fly vector and a mammalian host; understanding their life cycle is critical to understanding disease spread. One of the key life cycle stages is the haptomonad form, which attaches to insect tissues through its flagellum. This adhesion, conserved across kinetoplastid parasites, is implicated in having an important function within their life cycles and hence in disease transmission. Here, we discover the kinetoplastid-insect adhesion proteins (KIAPs), which localise in the attached Leishmania flagellum. Deletion of these KIAPs impairs cell adhesion in vitro and prevents Leishmania from colonising the stomodeal valve in the sand fly, without affecting cell growth. Additionally, loss of parasite adhesion in the sand fly results in reduced physiological changes to the fly, with no observable damage of the stomodeal valve and reduced midgut swelling. These results provide important insights into a comprehensive understanding of the Leishmania life cycle, which will be critical for developing transmission-blocking strategies.
• Left-right asymmetry is formed in the basal bodies of the mouse node cilia in a cilia motility-dependent manner
  Hiroshi Yoke; Atsushi Taniguchi; Shigenori Nonaka
  2023年09月14日
• Automated contour extraction for light‐sheet microscopy images of zebrafish embryos based on object edge detection algorithm
  Akiko Kondow; Kiyoshi Ohnuma; Atsushi Taniguchi; Joe Sakamoto; Makoto Asashima; Kagayaki Kato; Yasuhiro Kamei; Shigenori Nonaka
  Development, Growth & Differentiation, Wiley, 2023年06月23日, ［査読有り］
  研究論文（学術雑誌）
• Formation and three-dimensional architecture of Leishmania adhesion in the sand fly vector
  Ryuji Yanase; Flávia Moreira-Leite; Edward Rea; Lauren Wilburn; Jovana Sádlová; Barbora Vojtkova; Katerina Pružinová; Atsushi Taniguchi; Shigenori Nonaka; Petr Volf; Jack D Sunter
  eLife, 12, eLife Sciences Publications, Ltd, 2023年05月10日, ［査読有り］
  研究論文（学術雑誌）, Attachment to a substrate to maintain position in a specific ecological niche is a common strategy across biology, especially for eukaryotic parasites. During development in the sand fly vector, the eukaryotic parasite Leishmania adheres to the stomodeal valve, as the specialised haptomonad form. Dissection of haptomonad adhesion is a critical step for understanding the complete life cycle of Leishmania. Nevertheless, haptomonad studies are limited, as this is a technically challenging life cycle form to investigate. Here, we have combined three-dimensional electron microscopy approaches, including serial block face scanning electron microscopy (SBFSEM) and serial tomography to dissect the organisation and architecture of haptomonads in the sand fly. We showed that the attachment plaque contains distinct structural elements. Using time-lapse light microscopy of in vitro haptomonad-like cells, we identified five stages of haptomonad-like cell differentiation, and showed that calcium is necessary for Leishmania adhesion to the surface in vitro. This study provides the structural and regulatory foundations of Leishmania adhesion, which are critical for a holistic understanding of the Leishmania life cycle.
• First person – Atsushi Taniguchi and Yukinori Nishigami
  Biology Open, 12, 2, The Company of Biologists, 2023年02月15日
  研究論文（学術雑誌）, ABSTRACT
  
  First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping researchers promote themselves alongside their papers. Atsushi Taniguchi and Yukinori Nishigami are co-first authors on ‘ Light-sheet microscopy reveals dorsoventral asymmetric membrane dynamics of Amoeba proteus during pressure-driven locomotion’, published in BiO. Atsushi is a postdoc in the lab of Toshiyuki Nakagaki at Research Institute for Electronic Science, Hokkaido University, Kita-Ward Sapporo, Japan, investigating algorithms for collective space exploration and use in ciliates and amoebae. Yukinori is an assistant professor in the lab of Toshiyuki Nakagaki at the Research Institute for Electronic Science, Hokkaido University, Kita-Ward Sapporo, Japan, investigating the behavior of protists.
• Light-sheet microscopy reveals dorsoventral asymmetric membrane dynamics of Amoeba proteus during pressure-driven locomotion
  Atsushi Taniguchi; Yukinori Nishigami; Hiroko Kajiura-Kobayashi; Daisuke Takao; Daisuke Tamaoki; Toshiyuki Nakagaki; Shigenori Nonaka; Seiji Sonobe
  Biology Open, 12, 2, The Company of Biologists, 2023年01月30日, ［査読有り］, ［筆頭著者］, ［国際誌］
  研究論文（学術雑誌）, ABSTRACT
  
  Amoebae are found all around the world and play an essential role in the carbon cycle in the environment. Therefore, the behavior of amoebae is a crucial factor when considering the global environment. Amoebae change their distribution through amoeboid locomotion, which are classified into several modes. In the pressure-driven mode, intracellular hydrostatic pressure generated by the contraction of cellular cortex actomyosin causes the pseudopod to extend. During amoeboid locomotion, the cellular surface exhibits dynamic deformation. Therefore, to understand the mechanism of amoeboid locomotion, it is important to characterize cellular membrane dynamics. Here, to clarify membrane dynamics during pressure-driven amoeboid locomotion, we developed a polkadot membrane staining method and performed light-sheet microscopy in Amoeba proteus, which exhibits typical pressure-driven amoeboid locomotion. It was observed that the whole cell membrane moved in the direction of movement, and the dorsal cell membrane in the posterior part of the cell moved more slowly than the other membrane. In addition, membrane complexity varied depending on the focused characteristic size of the membrane structure, and in general, the dorsal side was more complex than the ventral side. In summary, the membrane dynamics of Amoeba proteus during pressure-driven locomotion are asymmetric between the dorsal and ventral sides.
  
  This article has an associated interview with the co-first authors of the paper.
• Temperature elevation was detected in migrating cells
  Takayuki Nakamura; Joe Sakamoto; Kohki Okabe; Atsushi Taniguchi; Takahiro G. Yamada; Shigenori Nonaka; Yasuhiro Kamei; Akira Funahashi; Makoto Tominaga; Noriko F. Hiroi
  Optics Continuum, 1, 5, 1085, 1097, Optica Publishing Group, 2022年04月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Near-wall rheotaxis of the ciliate Tetrahymena induced by the kinesthetic sensing of cilia
  Takuya Ohmura; Yukinori Nishigami; Atsushi Taniguchi; Shigenori Nonaka; Takuji Ishikawa; Masatoshi Ichikawa
  Science Advances, 7, 43, American Association for the Advancement of Science (AAAS), 2021年10月22日, ［査読有り］
  研究論文（学術雑誌）, Kinesthetic sensing of cilia results in upstream motility for Tetrahymena pyriformis , a typical freshwater microorganism.
• Light‐sheet microscopy‐based 3D single‐cell tracking reveals a correlation between cell cycle and the start of endoderm cell internalization in early zebrafish development
  Akiko Kondow; Kiyoshi Ohnuma; Yasuhiro Kamei; Atsushi Taniguchi; Ryoma Bise; Yoichi Sato; Hisateru Yamaguchi; Shigenori Nonaka; Keiichiro Hashimoto
  Development, Growth & Differentiation, 62, 7-8, 495, 502, Wiley, 2020年10月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Skeleton construction upon local regression of the sponge body
  Kouji Kishimoto; Wakana Sugano‐Yasunaga; Atsushi Taniguchi; Kiyokazu Agata; Shigenori Nonaka; Noriko Funayama
  Development, Growth & Differentiation, 61, 9, 485, 500, Wiley, 2019年12月09日, ［査読有り］
  研究論文（学術雑誌）, Abstract
  
  We previously revealed that the mechanism of demosponge skeleton construction is self‐organization by multiple rounds of sequential mechanical reactions of player cells. In these reactions, “transport cells” dynamically carry fine skeletal elements (spicules) on epithelia surrounding the inner body space of sponges (basal epithelium (basopinacoderm) and the endodermal epithelium (ENCM)). Once spicules pierce ENCM and apical pinacoderm, subsequently they are cemented to the substratum under the sponge body, or connected to other skeleton‐constructing spicules. Thus, the “pierce” step is the key to holding up spicules in the temporary periphery of growing sponges’ bodies. Since sponges can regress as well as grow, here we asked how skeleton construction occurs during local regression of the body. We found that prior to local basopinacoderm retraction (and thus body regression), the body became thinner. Some spicules that were originally carried outward stagnated for a while, and were then carried inwards either on ENCM or basopinacoderm. Spicules that were carried inwards on ENCM pierced epithelia after a short transport, and thus became held up at relatively inward positions compared to spicules carried on outwardly extending basopinacoderm. The switch of epithelia on which transport cells migrate efficiently occurred in thinner body spaces where basopinacoderm and ENCM became close to each other. Thus, the mechanisms underlying this phenomenon are rather mechanical: the combination of sequential reactions of skeleton construction and the narrowed body space upon local retraction of basopinacoderm cause spicules to be held up at more‐inward positions, which might strengthen the basopinacoderm's attachment to substratum.
• System level analysis of motor-related neural activities in larval Drosophila
  Youngteak Yoon; Jeonghyuk Park; Atsushi Taniguchi; Hiroshi Kohsaka; Ken Nakae; Shigenori Nonaka; Shin Ishii; Akinao Nose
  Journal of Neurogenetics, 33, 3, 179, 189, Informa UK Limited, 2019年06月07日, ［査読有り］
  研究論文（学術雑誌）
• Rotation of stress fibers as a single wheel in migrating fish keratocytes
  Okimura, C.; Taniguchi, A.; Nonaka, S.; Iwadate, Y.
  Scientific Reports, 8, 1, 2018年, ［査読有り］
  研究論文（学術雑誌）
• Influence of cellular shape on sliding behavior of ciliates
  Nishigami, Y.; Ohmura, T.; Taniguchi, A.; Nonaka, S.; Manabe, J.; Ishikawa, T.; Ichikawa, M.
  Communicative and Integrative Biology, 11, 4, 2018年, ［査読有り］
  研究論文（学術雑誌）
• Simple mechanosense and response of cilia motion reveal the intrinsic habits of ciliates
  Ohmura, T.; Nishigami, Y.; Taniguchi, A.; Nonaka, S.; Manabe, J.; Ishikawa, T.; Ichikawa, M.
  Proceedings of the National Academy of Sciences of the United States of America, 115, 13, 2018年, ［査読有り］
  研究論文（学術雑誌）
• Axially-confined in vivo single-cell labeling by primed conversion using blue and red lasers with conventional confocal microscopes
  Taniguchi, A.; Kimura, Y.; Mori, I.; Nonaka, S.; Higashijima, S.-I.
  Development Growth and Differentiation, 59, 9, 2017年, ［査読有り］
  研究論文（学術雑誌）
• High-speed microscopy with an electrically tunable lens to image the dynamics of in vivo molecular complexes
  Nakai, Y.; Ozeki, M.; Hiraiwa, T.; Tanimoto, R.; Funahashi, A.; Hiroi, N.; Taniguchi, A.; Nonaka, S.; Boilot, V.; Shrestha, R.; Clark, J.; Tamura, N.; Draviam, V.M.; Oku, H.
  Review of Scientific Instruments, 86, 1, 2015年, ［査読有り］
  研究論文（学術雑誌）
• High-Speed Imaging of Amoeboid Movements Using Light-Sheet Microscopy
  Takao, D.; Taniguchi, A.; Takeda, T.; Sonobe, S.; Nonaka, S.
  PLoS ONE, 7, 12, 2012年, ［査読有り］
  研究論文（学術雑誌）

• ウズツボカムリのチリモ摂取過程とワムシ付着による移動行動の報告
  釜屋憲彦; 西上幸範; 谷口篤史; 中垣俊之, 日本動物行動学会大会プログラム・要旨集(CD-ROM), 43rd, 2024年
• ウズツボカムリの餌環境依存的な探索行動と仮足動態変化
  釡屋憲彦; 中垣俊之; 谷口篤史; 西上幸範, 日本原生生物学会大会講演要旨集, 57th (Web), 2024年
• 車輪細胞見つけた！
  沖村 千夏; 谷口 篤史; 野中 茂紀; 岩楯 好昭, 生物物理, 59, 2, 94, 96, 2019年03月, ［査読有り］, ［招待有り］
  一般社団法人 日本生物物理学会, 日本語, 記事・総説・解説・論説等（学術雑誌）
• Amoeba proteusの単離細胞膜が示す自発曲率と生細胞三次元曲率に関する研究
  西上幸範; 谷口篤史; 野中茂紀; 園部誠司; 市川正敏, 日本原生生物学会大会講演要旨集, 47th, 2014年
• 自由生活型アメーバの単離細胞膜が示すRolling構造と細胞膜3次元曲率に関する研究
  西上幸範; 谷口篤史; 野中茂紀; 園部誠司; 市川正敏, 日本植物学会大会研究発表記録, 78th, 2014年
• Amoeba proteusの単離細胞膜が示すローリング構造に関する研究
  西上幸範; 谷口篤史; 野中茂紀; 市川正敏; 園部誠司, 日本原生動物学会プログラム講演要旨, 46th, 2013年

• 正しい結果を得るためのイメージング&画像解析実践テキスト : あなたの目的にあった顕微鏡の選択と撮像、定量解析フローの組み立て
  小山 宏史; 加藤 輝; 亀井 保博, 第Ⅰ部 画像取得 第2章 光の性質と光学顕微鏡 2 顕微鏡の光学理論の基礎 〜レンズを通る光のふるまいと開口数・倍率・分解能, 3 明視野顕微鏡法の種類 〜明視野観察法・偏斜照明法・暗視野観察法・位相差観察法・微分干渉観察法, 附録 2 F値, 3 散乱
  羊土社, 2024年05月, 9784758122719, 265p, 日本語
• 原生生物学事典
  矢﨑 裕規; 新倉 保; 猪飼 桂; 矢吹 彬憲; 永宗 喜三郎; 松崎 素道; 白鳥 峻志; 島野 智之; 小林 富美惠, 2. 分類と進化 2-2 アメーボゾア 1. ディスコセア, 2. ツブリネア
  朝倉書店, 2023年05月, 9784254171815, viii, 446p, 図版 [4] p, 日本語
• 達人に訊くバイオ画像取得と定量解析Q&A : 顕微鏡の設定からImageJによる解析・自動化まで
  加藤 輝; 小山 宏史, Q20, Q29, Q53, Q54, Q71, Q81
  羊土社, 2021年04月, 9784758122504, 218p, 日本語

• マウス胚ノード基底小体の周縁方向極性の解析
  科学研究費助成事業
  2014年04月01日 - 2017年03月31日
  野中 茂紀; 谷口 篤史
  哺乳類発生ではノード繊毛の運動が作り出す左向きの水流が将来の左右を決定する。この繊毛は時計回りに回転運動するがその角速度は左右非対称である。これが粘性抵抗による受動的なものか、繊毛構造に基づく能動的なものなのか、左右性の起源という観点から興味ある問題である。そこで繊毛基部にある基底小体の方向性を、そのマーカーとされるODF2の局在を調べることで決定しようとした。しかしODF2の局在が予想外のパターンを示したため所期の目的は達せられなかった。一方、母娘中心子の方向性に関してはランダムで、上記の粘性抵抗説を弱いながら支持する結果が得られた。
  日本学術振興会, 挑戦的萌芽研究, 基礎生物学研究所, 26650112