研究者基本情報

• 博士(医学), 北海道大学

• https://researchmap.jp/seijazzmas

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201801000823567632

• 201801000823567632

• 神経組織工学
• 神経病理

• ライフサイエンス, 分子生物学

研究活動情報

• 2018年09月, 日本神経病理学会, Young Investigator Award
  Development of bipolar charged hydrogel for neuronal tissue engineering.
  谷川 聖
• 2016年07月, 日本病理学会, 第13回日本病理学会カンファレンスポスター賞
  高分子ゲルが神経幹細胞へ与える影響
  谷川 聖

• Induction of Meningioma Stem Signature via Hydrogel Reprogramming and Application of Meningioma Stem Cell Marker CXCR4 to Pathological Diagnosis and Treatment.
  Yoshitaka Oda; Masumi Tsuda; Lei Wang; Jun Suzuka; Sayaka Yuzawa; Koki Ise; Umma Habiba; Jintao He; Satoshi Tanikawa; Hirokazu Sugino; Zen-Ichi Tanei; Christian Mawrin; Jian Ping Gong; Shinya Tanaka
  Biotechnology and bioengineering, 2026年02月27日, ［国際誌］
  英語, 研究論文（学術雑誌）, Meningiomas account for about 40% of all primary brain tumors. How ever effective treatments for recurrent or inoperable cases remain limited. We previously demonstrated that culturing cancer cells on specific hydrogels efficiently induces cancer stem cells across multiple cancer types, a process we termed hydrogel activated reprogramming (HARP) phenomenon. In this study, we aimed to identify key molecules involved in the induction of meningioma stem cells through hydrogel-based culture. Meningioma cells cultured on hydrogels were analyzed for expression of established stem cell markers and for tumorigenicity. Microarray analysis was performed to identify meningioma stem cell specific markers and to evaluate the application of these marker molecules as a therapeutic targets or as diagnostic tools for pathological grading. Canonical stem cell markers including Nanog, and Oct3/4 were upregulated in culturing meningioma cells on hydrogels. Comprehensive gene expression analysis identified some molecules involved in cancer stem cell activity among which CXCR4 was selected as a potential therapeutic target. Stimulation of CXCR4 with its ligand CXCL12 resulted in increased expression of stem cell markers. In human meningioma pathological specimens and cultured cell lines, there was a correlation between CXCR4 expression levels and NF2 mutations and/or deletions. CXCR4 immunohistochemistry was frequently positive in cases with brain invasion along with brain invasion area. These findings suggest that CXCR4 immunohistochemistry may be useful in suggesting typical CNS WHO grade 1 meningiomas without the need for molecular analysis. We have defined meningioma stem cell signature via HARP phenomenon and identified CXCR4 with biological significance as being diagnostic target. IMPORTANCE OF THE STUDY: In addition to morphological evaluation, immunohistochemistry and genetic alteration increasingly incorporated into the diagnostic criteria for central nervous system (CNS) tumors. From the CNS WHO 5th edition onwards, epigenetic features including DNA methylation profiling, have also been adopted as diagnostic criteria. In this study, we induced epigenetic changes in meningioma cells and successfully promoted cancer stemness highlighting the potential importance of this approach for both meningioma research and meningioma diagnostic development. Furthermore, microarray analysis identified CXCR4 as a molecule consistently upregulated during stem cell induction across all three hydrogel conditions. Subsequent analysis revealed that CXCR4 immunohistochemistry may reflect the distribution of meningioma stem cells, supporting its potential utility as a diagnostic marker. By integrating basic experimental findings with histopathological evaluation of clinical specimens, this report will contribute to the advancement of meningioma research and diagnostic strategies.
• SLC13A5 plays an essential role in the energy shift to oxidative phosphorylation in cisplatin-resistant mesothelioma stem cells.
  Marie Kato-Shinomiya; Hirokazu Sugino; Lei Wang; Yusuke Saito; Jintao He; Zen-Ichi Tanei; Yoshitaka Oda; Satoshi Tanikawa; Mishie Tanino; Jian Ping Gong; Masumi Tsuda; Shinya Tanaka
  Pathology international, 2025年02月06日, ［国際誌］
  英語, 研究論文（学術雑誌）, Mesothelioma is a highly aggressive tumor affecting an increasing number of patients worldwide. Owing to the poor clinical outcomes associated with current therapies, the development of novel therapies that target cancer stem cells (CSCs) is desirable. Here, we examined the applicability of our previously established hydrogel-based rapid CSC generation method to human mesothelioma cell lines and further analyzed the characteristics of the induced mesothelioma stem cell (MesoSC) -like cells. Human mesothelioma cell lines cultured on hydrogels presented increased expression of pan-stem cell markers and acquired spheroid formation and early tumorigenicity, suggesting that MesoSC-like cells are highly malignant. Microarray analysis demonstrated that the expression of SLC13A5, a citrate transporter involved in TCA cycle, was significantly induced in the resulting MesoSC-like cells. The overexpression of SLC13A5 resulted in a metabolic shift toward oxidative phosphorylation, increased phosphorylation of ERK and YAP, and increased SOX2 expression, leading to increased cisplatin resistance. scRNA-seq database analysis revealed that clinical mesothelioma samples contained a small number of SLC13A5-expressing cells. Our findings suggest that the hydrogel-based CSC generation method is also effective for human mesothelioma cells and that SLC13A5 may contribute to MesoSC survival. The new properties of MesoSCs revealed in this study may provide clues for establishing future treatments.
• Establishment of a novel method for differentiating into dopaminergic neurons using charged hydrogels.
  Bin Fan; Satoshi Tanikawa; Lei Wang; Takayuki Nonoyama; Yashitaka Oda; Zen-Ichi Tanei; Jian Ping Gong; Masumi Tsuda; Shinya Tanaka
  Biochemical and biophysical research communications, 747, 151280, 151280, 2025年02月02日, ［国際誌］
  英語, 研究論文（学術雑誌）, Parkinson's disease (PD) is a neurodegenerative disease primarily affecting the central nervous system and impacting both the motor system and non-motor systems. Although administration of L-DOPA is effective, it is not a fundamental treatment and has side effects such as diurnal fluctuation and dyskinesia, highlighting the need for new treatment methods. There is a growing interest in dopaminergic neuron transplantation as a potential treatment. Dopaminergic neurons derived from pluripotent stem (iPS) cells provide a valuable source for transplantation therapies. Developing an efficient method to differentiate iPS cells into dopaminergic cells is essential for cell transplantation therapy. While Cell differentiation is typically controlled by the addition of specific reagents, the physical characteristics of culture substrate, especially in the charge and stiffness, are also crucial factors in regulating differentiation. In this research, we show that two newly developed electrically charged polymeric hydrogels composed of cationic (C) and anionic (A) monomers inratio of 1-9 and 2 to 8 can significantly promote Dopaminergic neuron differentiation. Our findings emphasize the importance of culture substrates in effective dopaminergic cell differentiation.
• Pathological study of progressive supranuclear palsy the cases with mutations in Bassoon
  Masahiro Wakita; Hiroaki Yaguchi; Mika Otuski; Satoshi Tanikawa; Yasuo Miki; Ikuko Aiba; Mari Yoshida; Taichi Nomura; Hisashi Uwatoko; Yasunori Mito; Kazuyoshi Sinpo; Takeshi Ikeuchi; Shinya Tanaka; Koichi Wakabayashi; Ichiro Yabe
  Neuropathology, Wiley, 2024年10月31日
  研究論文（学術雑誌）, Clinical diagnosis of progressive supranuclear palsy (PSP) is difficult due to various phenotypes. Neuropathologically, PSP is defined by neuronal loss in the basal ganglia and brainstem with widespread occurrence of neurofibrillary tangles (NFTs) and accumulation of phosphorylated tau protein in neurons and glial cells in the brain. We previously identified the point mutation p.Pro3866Ala in the Bassoon (BSN) gene in a Japanese family with PSP‐like syndrome. We newly detected BSN mutations in two autopsied PSP cases carrying p.Thr2542Met and p.Glu2759Gly, respectively. The case with p.Thr2542Met mutation showed neurological symptoms including behavioral abnormalities, cognitive dysfunction, and parkinsonism. Brain magnetic resonance imaging (MRI) showed atrophy of the midbrain tegmentum and hippocampus. Pathologically, moderate to severe loss of neurons with gliosis was also found in the substantia nigra, and there was an almost complete loss of neurons with gliosis in the transitional zone of the cornu ammonis (CA) 1 region to the subiculum. NFTs were observed in the globus pallidus, subthalamic nucleus, substantia nigra, and CA1. 4R tau‐dominant tauopathy was detected. The case with p.Glu2759Gly mutation showed neurological symptoms, including right‐dominant motor impairment, right limping gait, postural instability, and cognitive dysfunction. Brain MRI showed mild atrophy of the midbrain tegmentum and left‐dominant parietal lobe atrophy. Pathologically, NFTs were detected in the globus pallidus, subthalamic nucleus, substantia nigra, thalamus, putamen, and brainstem tegmentum. Most neurons were immunopositive for four‐repeat tau, whereas only a few of them harbored three‐repeat tau‐positive NFTs in the hippocampus. We showed the results of a pathological study of PSP cases with BSN mutations; these were two new cases. The clinical phenotypes were similar to the first case in the point of neurological symptoms. Accumulation of four‐repeat tau was dominant. Further autopsies of BSN mutation cases and further elucidation of the molecular biological mechanism are desirable.
• Conventional magnetic resonance imaging key features for distinguishing pathologically confirmed corticobasal degeneration from its mimics: a retrospective analysis of the J-VAC study.
  Keita Sakurai; Aya M Tokumaru; Mari Yoshida; Yuko Saito; Koichi Wakabayashi; Takashi Komori; Masato Hasegawa; Takeshi Ikeuchi; Yuichi Hayashi; Takayoshi Shimohata; Shigeo Murayama; Yasushi Iwasaki; Toshiki Uchihara; Motoko Sakai; Ichiro Yabe; Satoshi Tanikawa; Hiroshi Takigawa; Tadashi Adachi; Ritsuko Hanajima; Harutoshi Fujimura; Kentaro Hayashi; Keizo Sugaya; Kazuko Hasegawa; Terunori Sano; Masaki Takao; Osamu Yokota; Tomoko Miki; Michio Kobayashi; Nobutaka Arai; Takuya Ohkubo; Takanori Yokota; Keiko Mori; Masumi Ito; Chiho Ishida; Jiro Idezuka; Yasuko Toyoshima; Masato Kanazawa; Masashi Aoki; Takafumi Hasegawa; Hirohisa Watanabe; Atsushi Hashizume; Hisayoshi Niwa; Keizo Yasui; Keita Ito; Yukihiko Washimi; Akatsuki Kubota; Tatsushi Toda; Kenji Nakashima; Ikuko Aiba
  Neuroradiology, 2024年07月22日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, PURPOSE: Due to the indistinguishable clinical features of corticobasal syndrome (CBS), the antemortem differentiation between corticobasal degeneration (CBD) and its mimics remains challenging. However, the utility of conventional magnetic resonance imaging (MRI) for the diagnosis of CBD has not been sufficiently evaluated. This study aimed to investigate the diagnostic performance of conventional MRI findings in differentiating pathologically confirmed CBD from its mimics. METHODS: Semiquantitative visual rating scales were employed to assess the degree and distribution of atrophy and asymmetry on conventional T1-weighted and T2-weighted images. Additionally, subcortical white matter hyperintensity (SWMH) on fluid-attenuated inversion recovery images were visually evaluated. RESULTS: In addition to 19 patients with CBD, 16 with CBD mimics (progressive supranuclear palsy (PSP): 9, Alzheimer's disease (AD): 4, dementia with Lewy bodies (DLB): 1, frontotemporal lobar degeneration with TAR DNA-binding protein of 43 kDa(FTLD-TDP): 1, and globular glial tauopathy (GGT): 1) were investigated. Compared with the CBD group, the PSP-CBS subgroup showed severe midbrain atrophy without SWMH. The non-PSP-CBS subgroup, comprising patients with AD, DLB, FTLD-TDP, and GGT, showed severe temporal atrophy with widespread asymmetry, especially in the temporal lobes. In addition to over half of the patients with CBD, two with FTLD-TDP and GGT showed SWMH, respectively. CONCLUSION: This study elucidates the distinct structural changes between the CBD and its mimics based on visual rating scales. The evaluation of atrophic distribution and SWMH may serve as imaging biomarkers of conventional MRI for detecting background pathologies.
• A Case of Uterine Tumor Resembling Ovarian Sex Cord Tumor With Prominent Myxoid Features.
  Koki Ise; Zen-Ichi Tanei; Yoshitaka Oda; Satoshi Tanikawa; Hirokazu Sugino; Yusuke Ishida; Masumi Tsuda; Yuko Gotoda; Kunihiko Nishiwaki; Hiroyuki Yanai; Tadashi Hasegawa; Kazuo Nagashima; Shinya Tanaka
  International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, 2023年06月14日, ［国際誌］
  英語, 研究論文（学術雑誌）, Uterine tumor resembling ovarian sex cord tumor (UTROSCT) is a rare tumor with low malignant potential that commonly occurs in middle age. Although more than 100 cases have been reported to date, myxoid morphology is not well documented. Here, we present a 75-yr-old woman with abnormal vaginal bleeding, with an 8-cm mass in the uterine corpus detected by irregular, high-intensity signaling on T2-weighted imaging. The uterine mass had a glistening mucinous appearance on gross examination. Microscopically, most of the tumor cells were floating in the myxoid stroma. The tumor cells formed clusters or nests with abundant cytoplasm, while some exhibited trabecular or rhabdoid appearances. Immunohistochemically, tumor cells were positive for pancytokeratin (AE1/AE3), α-smooth muscle actin, CD10, progesterone receptor, and some sex cord markers such as calretinin, inhibin, CD56, steroidogenic factor-1. Electron microscopy demonstrated epithelial and sex cord differentiation. This tumor was negative for JAZF1-JJAZ1 fusion gene that is frequently found in low-grade endometrial stromal sarcoma. Fusion genes related to UTROSCT, including NCOA2/3, were not detected by reverse transcription polymerase chain reaction. The present case suggests that UTROSCT should be included in the differential diagnosis of myxoid uterine tumors.
• 進行性核上性麻痺にTDP43 pathologyを合併した一剖検例
  鍵谷 豪太; 種井 善一; 谷川 聖; 小田 義崇; 王 磊; 津田 真寿美; 大槻 美佳; 田中 伸哉
  日本病理学会会誌, 112, 1, 379, 379, (一社)日本病理学会, 2023年03月
  日本語
• 拡張型心筋症を発症したEmery-Dreifuss型筋ジストロフィーの一剖検例
  佐々木 美羽; 種井 善一; 松島 理明; 石垣 隆弘; 桑原 健; 小田 義嵩; 谷川 聖; 津田 真寿美; 矢部 一郎; 田中 伸哉
  日本病理学会会誌, 112, 1, 381, 381, (一社)日本病理学会, 2023年03月
  日本語
• TKIs耐性膠芽腫細胞の特性と耐性メカニズムの解析
  津田 真寿美; 王 磊; 小田 義崇; 谷川 聖; 種井 善一; 田中 伸哉
  日本病理学会会誌, 112, 1, 278, 278, (一社)日本病理学会, 2023年03月
  日本語
• 72歳女性のMultinodular and Vacuolating Neuronal Tumor of the cerebrumの1例
  寺島 祐樹; 種井 善一; 浅野目 卓; 黒田 花音; 小田 義崇; 谷川 聖; 王 磊; 津田 真寿美; 佐藤 憲市; 田中 伸哉
  日本病理学会会誌, 112, 1, 375, 375, (一社)日本病理学会, 2023年03月
  日本語
• JCVとCMVの脳幹部重複感染症例におけるウイルスの局在解析
  黒田 花音; 種井 善一; 岡崎 ななせ; 工藤 彰彦; 阿部 恵; 寺島 祐樹; 谷川 聖; 津田 真寿美; 矢部 一郎; 田中 伸哉
  日本病理学会会誌, 112, 1, 375, 375, (一社)日本病理学会, 2023年03月
  日本語
• 慢性血栓塞栓性肺高血圧症の一剖検例
  岸本 佳子; 種井 善一; 青木 健志; 加藤 万里絵; 小田 義崇; 谷川 聖; 津田 真寿美; 田中 伸哉
  日本病理学会会誌, 112, 1, 378, 378, (一社)日本病理学会, 2023年03月
  日本語
• Becker型筋ジストロフィーの兄弟剖検症例の病理組織学的検討
  宮本 裕也; 種井 善一; 谷川 聖; 小田 義崇; 津田 真寿美; 加納 崇裕; 横田 卓; 矢部 一郎; 田中 伸哉
  日本病理学会会誌, 112, 1, 378, 378, (一社)日本病理学会, 2023年03月
  日本語
• 髄膜腫の骨化におけるEpithelial-mesenchymal transitionの関与についての検討
  京野 里虹; 種井 善一; 寺島 祐樹; 小田 義崇; 谷川 聖; 王 磊; 津田 真寿美; 田中 伸哉
  日本病理学会会誌, 112, 1, 381, 381, (一社)日本病理学会, 2023年03月
  日本語
• FFPE検体の質量分析による肺小細胞癌の脳転移関連分子の解析
  江端 美織; 何 錦涛; 小田 義崇; 谷川 聖; 王 磊; 津田 真寿美; 種井 善一; 田中 伸哉
  日本病理学会会誌, 112, 1, 383, 383, (一社)日本病理学会, 2023年03月
  日本語
• 拡張型心筋症を発症したEmery-Dreifuss型筋ジストロフィーの一剖検例
  佐々木 美羽; 種井 善一; 松島 理明; 石垣 隆弘; 桑原 健; 小田 義嵩; 谷川 聖; 津田 真寿美; 矢部 一郎; 田中 伸哉
  日本病理学会会誌, 112, 1, 381, 381, (一社)日本病理学会, 2023年03月
  日本語
• An autopsy case report of adult-onset Krabbe disease: Comparison with an infantile-onset case.
  Miu Sasaki; Miori Ebata; Zen-Ichi Tanei; Yoshitaka Oda; Akiko Hamauchi; Satoshi Tanikawa; Hirokazu Sugino; Yusuke Ishida; Takenori Abe; Nobutaka Arai; Kazuya Sako; Shinya Tanaka
  Pathology international, 2022年10月06日, ［国際誌］
  英語, Krabbe disease is a lysosomal storage disease caused by a deficiency of the galactocerebrosidase (GALC) enzyme, which leads to demyelination of the central and peripheral nervous systems. Almost all patients with Krabbe disease are infants, and this is the first report of adult-onset cases that describe pathological findings. Here, we present two autopsy cases: a 73-year-old female and a 2-year-old male. The adult-onset case developed symptoms in her late thirties and was diagnosed by the identification of GALC D528N and L634S mutations and by T2-weighted magnetic resonance imaging; she had increased signal in the white matter along the pyramidal tract to the bilateral precentral gyrus, as well as from the triangular part to the posterior horn of the lateral ventricle. Microscopically, Klüver-Barrera staining was pale in the white matter of the precentral gyrus and occipito-thalamic radiation, and a few globoid cells were observed. The GALC mutations that were identified in the present adult-onset case do not completely inactivate GALC enzyme activity, resulting in focal demyelination of the brain.
• 脊髄capillary hemangiomaの病理像
  種井 善一; 津田 真寿美; 小田 義崇; 谷川 聖; 杉野 弘和; 大竹 安史; 今村 博幸; 小柳 泉; 飛騨 一利; 田中 伸哉
  日本病理学会会誌, 111, 1, 264, 264, (一社)日本病理学会, 2022年03月
  日本語
• びまん性大細胞型B細胞性リンパ腫の化学療法中に突然死した1剖検例
  加藤 万里絵; 種井 善一; 小島 圭祐; 太田 秀一; ウンマ・ハビバ; 小田 義崇; 谷川 聖; 杉野 弘和; 津田 真寿美; 田中 伸哉
  日本病理学会会誌, 111, 1, 308, 308, (一社)日本病理学会, 2022年03月
  日本語
• 内頸動脈瘤術後に急激な意識障害をきたした神経線維腫症1型の一剖検例
  京野 里虹; 種井 善一; 岡崎 ななせ; 長内 俊也; 小田 義崇; 谷川 聖; 杉野 弘和; 津田 真寿美; 藤村 幹; 田中 伸哉
  日本病理学会会誌, 111, 1, 356, 356, (一社)日本病理学会, 2022年03月
  日本語
• SLEとGood症候群の治療経過中に脳幹障害を来たした一例
  黒田 花音; 種井 善一; 岡崎 ななせ; 工藤 彰彦; 阿部 恵; 小田 義崇; 谷川 聖; 杉野 弘和; 矢部 一郎; 田中 伸哉
  日本病理学会会誌, 111, 1, 356, 356, (一社)日本病理学会, 2022年03月
  日本語
• 細胞外基質の電位変化に伴うJCウイルス増殖の制御
  谷川 聖; 野々山 貴行; 津田 真寿美; 王 磊; 種井 善一; Gong Jian Ping; 田中 伸哉
  日本病理学会会誌, 111, 1, 263, 263, (一社)日本病理学会, 2022年03月
  日本語
• 脊髄capillary hemangiomaの病理像
  種井 善一; 津田 真寿美; 小田 義崇; 谷川 聖; 杉野 弘和; 大竹 安史; 今村 博幸; 小柳 泉; 飛騨 一利; 田中 伸哉
  日本病理学会会誌, 111, 1, 264, 264, (一社)日本病理学会, 2022年03月
  日本語
• 内頸動脈瘤術後に急激な意識障害をきたした神経線維腫症1型の一剖検例
  京野 里虹; 種井 善一; 岡崎 ななせ; 長内 俊也; 小田 義崇; 谷川 聖; 杉野 弘和; 津田 真寿美; 藤村 幹; 田中 伸哉
  日本病理学会会誌, 111, 1, 356, 356, (一社)日本病理学会, 2022年03月
  日本語
• SLEとGood症候群の治療経過中に脳幹障害を来たした一例
  黒田 花音; 種井 善一; 岡崎 ななせ; 工藤 彰彦; 阿部 恵; 小田 義崇; 谷川 聖; 杉野 弘和; 矢部 一郎; 田中 伸哉
  日本病理学会会誌, 111, 1, 356, 356, (一社)日本病理学会, 2022年03月
  日本語
• Anti-myelin oligodendrocyte glycoprotein antibody-associated encephalitis with cortical hyperintensity and pathologically confirmed extensive demyelination
  Kosuke Iwami; Ikuko Takahashi-Iwata; Katsuki Eguchi; Azusa Nagai; Shinichi Shirai; Masaaki Matsushima; Hiroaki Yaguchi; Shinichi Nakazato; Satoshi Tanikawa; Yoshiaki Tagawa; Yasuhiro Shinmei; Toshiyuki Takahashi; Shinya Tanaka; Ichiro Yabe
  Neuroimmunology Reports, 1, 100032, 100032, Elsevier BV, 2021年12月, ［査読有り］
  研究論文（学術雑誌）
• 非流暢/失文法型原発性進行性失語(naPPA)を呈したPick病
  大槻 美佳; 谷川 聖; 中川 賀嗣; 廣谷 真; 江口 克紀; 白井 慎一; 岩田 育子; 松島 理明; 脇田 雅大; 芳野 正修; 大嶌 祐貴; 水島 慶一; 田中 伸哉; 佐々木 秀直; 矢部 一郎
  日本神経心理学会総会プログラム・予稿集, 45回, 112, 112, 日本神経心理学会, 2021年09月
  日本語
• Phlegmonous gastritis developed during chemotherapy for acute lymphocytic leukemia: A case report.
  Makoto Saito; Masanobu Morioka; Koh Izumiyama; Akio Mori; Reiki Ogasawara; Takeshi Kondo; Toru Miyajima; Emi Yokoyama; Satoshi Tanikawa
  World journal of clinical cases, 9, 22, 6493, 6500, 2021年08月06日, ［査読有り］, ［最終著者, 責任著者］, ［国際誌］
  英語, BACKGROUND: Phlegmonous gastritis (PG) is a rare bacterial infectious disease characterized by neutrophil-based purulent inflammation of the gastric wall. The most representative causative bacterium is Streptococcus pyogenes, followed by Staphylococcus, Pneumococcus and Enterococcus. Hepatic portal venous gas (HPVG) is considered a potentially fatal condition and is rarely associated with PG. CASE SUMMARY: The white blood cell count of a 70-year-old woman with acute lymphocytic leukemia in complete remission dropped to 100/μL after consolidation chemotherapy. Her vital signs were consistent with septic shock. Venous blood culture revealed the presence of Bacillus cereus. Abdominal computed tomography (CT) and esophagogastroduodenoscopy (EGD) showed marked thickening of the gastric wall. As with the other findings, CT was suggestive of HPVG, and EGD showed pseudomembrane-like tissue covering the superficial mucosa. Histopathological examination of gastric biopsy specimens showed mostly necrotic tissue with lymphocytes rather than neutrophils. Culture of gastric specimens revealed the presence of Bacillus cereus. We finally diagnosed this case as PG with Bacillus cereus-induced sepsis and HPVG. This patient recovered successfully with conservative treatment, chiefly by using carbapenem antibiotics. CONCLUSION: The histopathological finding of this gastric biopsy specimen should be called "neutropenic necrotizing gastritis".
• Loss of H3K27 trimethylation is frequent in IDH1-R132H but not in non-canonical IDH1/2 mutated and 1p/19q codeleted oligodendroglioma: a Japanese cohort study.
  Umma Habiba; Hirokazu Sugino; Roumyana Yordanova; Koki Ise; Zen-Ichi Tanei; Yusuke Ishida; Satoshi Tanikawa; Shunsuke Terasaka; Ken-Ichi Sato; Yuuta Kamoshima; Masahiko Katoh; Motoo Nagane; Junji Shibahara; Masumi Tsuda; Shinya Tanaka
  Acta neuropathologica communications, 9, 1, 95, 95, 2021年05月21日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, Oligodendrogliomas are defined by mutation in isocitrate dehydrogenase (NADP(+)) (IDH)1/2 genes and chromosome 1p/19q codeletion. World Health Organisation diagnosis endorses testing for 1p/19q codeletion to distinguish IDH mutant (Mut) oligodendrogliomas from astrocytomas because these gliomas require different treatments and they have different outcomes. Several methods have been used to identify 1p/19q status; however, these techniques are not routinely available and require substantial infrastructure investment. Two recent studies reported reduced immunostaining for trimethylation at lysine 27 on histone H3 (H3K27me3) in IDH Mut 1p/19q codeleted oligodendroglioma. However, the specificity of H3K27me3 immunostaining in this setting is controversial. Therefore, we developed an easy-to-implement immunohistochemical surrogate for IDH Mut glioma subclassification and evaluated a validated adult glioma cohort. We screened 145 adult glioma cases, consisting of 45 IDH Mut and 1p/19q codeleted oligodendrogliomas, 30 IDH Mut astrocytomas, 16 IDH wild-type (Wt) astrocytomas, and 54 IDH Wt glioblastomas (GBMs). We compared immunostaining with DNA sequencing and fluorescent in situ hybridization analysis and assessed differences in H3K27me3 staining between oligodendroglial and astrocytic lineages and between IDH1-R132H and non-canonical (non-R132H) IDH1/2 Mut oligodendroglioma. A loss of H3K27me3 was observed in 36/40 (90%) of IDH1-R132H Mut oligodendroglioma. In contrast, loss of H3K27me3 was never seen in IDH1-R132L or IDH2-mutated 1p/19q codeleted oligodendrogliomas. IDH Mut astrocytoma, IDH Wt astrocytoma and GBM showed preserved nuclear staining in 87%, 94%, and 91% of cases, respectively. A high recursive partitioning model predicted probability score (0.9835) indicated that the loss of H3K27me3 is frequent to IDH1-R132H Mut oligodendroglioma. Our results demonstrate H3K27me3 immunohistochemical evaluation to be a cost-effective and reliable method for defining 1p/19q codeletion along with IDH1-R132H and ATRX immunostaining, even in the absence of 1p/19q testing.
• 脊髄capillary hemangiomaの臨床病理学的特徴
  種井 善一; 津田 真寿美; 谷川 聖; 杉野 弘和; 石田 雄介; 大竹 安史; 今村 博幸; 小柳 泉; 飛騨 一利; 田中 伸哉
  Brain Tumor Pathology, 38, Suppl., 119, 119, 日本脳腫瘍病理学会, 2021年05月
  日本語
• 成人型Krabbe病の一剖検例
  佐々木 美羽; 江端 美織; 小田 義崇; 濱内 朗子; 谷川 聖; 種井 善一; 杉野 弘和; 石田 雄介; 佐光 一也; 田中 伸哉
  日本病理学会会誌, 110, 1, 379, 379, (一社)日本病理学会, 2021年03月
  日本語
• バイオマテリアルによる肝癌幹細胞の新規誘導法の開発とその解析
  谷 道夫; 津田 真寿美; 鈴鹿 淳; 王 磊; 杉野 弘和; 谷川 聖; 石田 雄介; グン 剣萍; 田中 伸哉; 武冨 紹信
  日本外科学会定期学術集会抄録集, 120回, DP, 6, (一社)日本外科学会, 2020年08月
  日本語
• バイオマテリアルによる肝癌幹細胞の新規誘導法の開発とその解析
  谷 道夫; 津田 真寿美; 鈴鹿 淳; 王 磊; 杉野 弘和; 谷川 聖; 石田 雄介; グン 剣萍; 田中 伸哉; 武冨 紹信
  日本外科学会定期学術集会抄録集, 120回, DP, 6, (一社)日本外科学会, 2020年08月
  日本語
• Signaling adaptor protein Crk is involved in malignant feature of pancreatic cancer associated with phosphorylation of c-Met.
  Satoko Uemura; Lei Wang; Masumi Tsuda; Jun Suzuka; Satoshi Tanikawa; Hirokazu Sugino; Toru Nakamura; Tomoko Mitsuhashi; Satoshi Hirano; Shinya Tanaka
  Biochemical and biophysical research communications, 524, 2, 378, 384, 2020年04月02日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, Signaling adaptor protein Crk has been shown to play an important role in various human cancers. Crk links tyrosine kinases and guanine nucleotide exchange factors (GEFs) such as C3G and Dock180 to activate small G-proteins Rap and Rac, respectively. In pancreatic cancer, various molecular targeted therapies have provided no significant therapeutic benefit for the patients so far due to constitutive activation of KRAS by frequent KRAS mutation. Therefore, the establishment of novel molecular targeted therapy in KRAS-independent manner is required. Here, we investigated a potential of Crk as a therapeutic target in pancreatic cancer. Immunohistochemistry on human pancreatic cancer specimens revealed that the patients with high expression of Crk had a worse prognosis than those with low expression. We established Crk-knockdown pancreatic cancer cells by siRNA using PANC-1, AsPC-1, and MIA PaCa-2 cells, which showed decreased cell proliferation, invasion, and adhesion. In Crk-knockdown pancreatic cancer cells, the decrease of c-Met phosphorylation was observed. In the orthotopic xenograft model, Crk depletion prolonged survival of mice significantly. Thus, signaling adaptor protein Crk is involved in malignant potential of pancreatic cancer associated with decrease of c-Met phosphorylation, and Crk can be considered to be a potential therapeutic molecular target.
• Emery-Dreifuss型筋ジストロフィーに伴う心不全の1剖検例
  五味川 龍; 石田 雄介; 桑原 健; 石垣 隆弘; 谷川 聖; 小田 義崇; 王 磊; 杉野 弘和; 津田 真寿美; 田中 伸哉
  日本病理学会会誌, 109, 1, 492, 492, (一社)日本病理学会, 2020年03月
  日本語
• 前立腺癌のGleason pattern評価のためのSemantic segmentationモデル及びRaspberry Pi端末を用いた応用
  遠田 建; 伊勢 昂生; 石田 雄介; 桑原 健; 谷川 聖; 小田 義崇; 王 磊; 杉野 弘和; 津田 真寿美; 田中 伸哉
  日本病理学会会誌, 109, 1, 496, 496, (一社)日本病理学会, 2020年03月
  日本語
• 新規変異BRAFV601K変異を認める良性脳腫瘍(毛様体性星細胞腫)の一例
  榎枝 未紗; 小田 義崇; 津田 真寿美; 飛弾 一利; 杉野 弘和; 谷川 聖; 鈴鹿 淳; 王 磊; 石田 雄介; 田中 伸哉
  日本病理学会会誌, 109, 1, 497, 497, (一社)日本病理学会, 2020年03月
  日本語
• MM2 cortical form of sporadic Creutzfeldt-Jakob disease without progressive dementia and akinetic mutism: A case deviating from current diagnostic criteria.
  Ikuko Takahashi-Iwata; Ichiro Yabe; Akihiko Kudo; Katsuya Eguchi; Masahiro Wakita; Shinichi Shirai; Masaaki Matsushima; Takanobu Toyoshima; Susumu Chiba; Satoshi Tanikawa; Shinya Tanaka; Katsuya Satoh; Tetsuyuki Kitamoto; Hidenao Sasaki
  Journal of the neurological sciences, 412, 116759, 116759, 2020年02月24日, ［査読有り］, ［国際誌］
  英語
• Autopsy findings in the early stage of amyotrophic lateral sclerosis with "dropped head" syndrome.
  Satoshi Tanikawa; Mishie Tanino; Lei Wang; Marin Ishikawa; Masaya Miyazaki; Masumi Tsuda; Yasuko Orba; Hirofumi Sawa; Kotarou Matoba; Nishio Nakamura; Kazuo Nagashima; William W Hall; Shinya Tanaka
  Neuropathology : official journal of the Japanese Society of Neuropathology, 39, 5, 374, 377, 2019年10月, ［査読有り］, ［筆頭著者］, ［国際誌］
  英語, Dropped head syndrome (DHS) has been rarely observed in amyotrophic lateral sclerosis (ALS), and the neuropathological findings of this condition have almost never been described. The identification of transactivation response DNA-binding protein 43 kDa (TDP-43), which binds to RNA/DNA has provided a new method for studying ALS and frontotemporal lobar degeneration (FTLD). Post-mortem examination of an adult sudden death case of a 71-year-old patient who complained of DHS exhibited severe loss of anterior motor neurons in the cervical cord (C4-6). Loss of nerve fibers of the anterior roots was striking compared with posterior roots, together with marked neurogenic atrophy of posterior muscles semispinalis cervicis. Bunina bodies were found in large neurons of Betz giant cells, but not in the motor neurons of spinal cords, or neurons of bulbar regions. Phosphorylated TDP-43 (p-TDP-43)-positive structures were detected in the residual neurons of the cervical, thoracic and lumber cords, hypoglossal nucleus, cerebellar dentate nucleus and parahippocampal cortex, together with ubiquitin-positive inclusions. Phosphorylated Tau positive structures in neuronal cytoplasm were found in the amygdala, entorhinal cortex and parahippocampal cortex, some of which co-expressed p-TDP-43. The medial zone of cervical cords may be the first onset site, and that is the cause of head drop in the early stage of ALS. In spite of detailed examination, the direct cause of sudden death was not verified. This autopsy report revealed the relation of DHS which is a rare clinical manifestation of ALS, and neuropathological findings.
• A 41 Year-Old Woman with a Mass in the Posterior Cranial Fossa.
  Bunsho Asayama; Yoshinobu Seo; Yoshimaru Ozaki; Satoshi Tanikawa; Takanori Hirose; Shinya Tanaka; Hirohiko Nakamura
  Brain Pathology, 29, 5, 699, 700, 2019年08月, ［査読有り］
  英語
• バイオマテリアルによる肝癌幹細胞の新規誘導法
  谷 道夫; 津田 真寿美; 鈴鹿 淳; 王 磊; 杉野 弘和; 谷川 聖; 石田 雄介; グン 剣萍; 田中 伸哉; 武冨 紹信
  日本外科学会定期学術集会抄録集, 119回, PS, 6, (一社)日本外科学会, 2019年04月
  日本語
• 病理IT化、情報化とAI研究 病理診断と人工知能 スタートアップから脳腫瘍への応用まで
  石田 雄介; 桑原 健; 小田 義崇; 谷川 聖; 杉野 弘和; 津田 真寿美; 田中 伸哉
  日本病理学会会誌, 108, 1, 192, 192, (一社)日本病理学会, 2019年04月
  日本語
• 解剖で偶然見つかった無症候性胸髄内神経鞘腫の一例
  有田 梨乃; 谷川 聖; 津田 真寿美; 石田 雄介; 杉野 弘和; 田中 伸哉
  日本病理学会会誌, 108, 1, 459, 459, (一社)日本病理学会, 2019年04月
  日本語
• 髄膜腫におけるTERTプロモーター遺伝子変異の検討
  久世 瑞穂; 小田 義崇; 津田 真寿美; 湯澤 明夏; 谷川 聖; 杉野 弘和; 石田 雄介; 田中 伸哉
  日本病理学会会誌, 108, 1, 463, 463, (一社)日本病理学会, 2019年04月
  日本語
• Autopsy report of a late delayed radiation injury after a period of 45 years.
  Satoshi Tanikawa; Yasutaka Kato; Mishie Tanino; Shunsuke Terasaka; Yasuo Kurokawa; Nobutaka Arai; Kazuo Nagashima; Shinya Tanaka
  Neuropathology : official journal of the Japanese Society of Neuropathology, 39, 2, 106, 110, 2019年04月, ［査読有り］, ［筆頭著者, 責任著者］, ［国際誌］
  英語, For delayed radiation injury, image analysis has considerably advanced, but neuropathological findings are still required to establish diagnosis. A patient who had received radiation therapy for pineal germinoma at age 14 developed neurological and psychiatric abnormalities after 15 years as a late delayed radiation injury. Autopsy at age 59 revealed diffuse changes in the white matter consisting in order of severity of myelin pallor, demyelination, and necrosis which were characterized by a lack of glial reaction. The cerebral cortex was relatively well preserved. As delayed radiation injuries, hyalinous changes in the vascular wall, angiomatous lesions and, fresh and old petechial hemorrhages were found. Moreover, vascular changes associated with arteriosclerosis were also present. Furthermore, a focal glial nodule was detected which was considered to be a new radiation-induced neoplasia. These findings suggest that late delayed radiation injury may slowly develop over 30 years and may involve damage to neuroglial stem cell compensation. It is also evident that arteriosclerotic changes and newly induced neoplasia may develop in delayed radiation injury cases.
• 馬尾原発の髄外性形質細胞腫の1例
  小田 義崇; 杉野 弘和; 小柳 泉; 谷川 聖; 石田 雄介; 津田 真寿美; 田中 伸哉
  Brain Tumor Pathology, 35, Suppl., 177, 177, 日本脳腫瘍病理学会, 2018年09月
  日本語
• Hepatobiliary and Pancreatic: Pancreatic cancer with elevated serum IgG4 level due to multiple myeloma mimicking localized autoimmune pancreatitis
  Shin Kato; Masaki Kuwatani; Kazumichi Kawakubo; Ryo Sugiura; Koji Hirata; Satoshi Tanikawa; Tomoko Mitsuhashi; Souichi Shiratori; Naoya Sakamoto
  Journal of Gastroenterology and Hepatology, 33, 7, 1310, 1310, Wiley, 2018年07月, ［査読有り］
  研究論文（学術雑誌）
• 神経再生工学における両電荷を有するハイドロゲルの開発
  谷川 聖; 仙葉 慎吾; 津田 真寿美; 王 磊; 谷野 美智枝; 石田 雄介; 杉野 弘和; 鈴鹿 淳; 田中 伸哉
  日本病理学会会誌, 107, 1, 329, 329, (一社)日本病理学会, 2018年04月
  日本語
• Mutations in bassoon in individuals with familial and sporadic progressive supranuclear palsy-like syndrome.
  Ichiro Yabe; Hiroaki Yaguchi; Yasutaka Kato; Yasuo Miki; Hidehisa Takahashi; Satoshi Tanikawa; Shinichi Shirai; Ikuko Takahashi; Mari Kimura; Yuka Hama; Masaaki Matsushima; Shinsuke Fujioka; Takahiro Kano; Masashi Watanabe; Shin Nakagawa; Yasuyuki Kunieda; Yoshio Ikeda; Masato Hasegawa; Hiroshi Nishihara; Toshihisa Ohtsuka; Shinya Tanaka; Yoshio Tsuboi; Shigetsugu Hatakeyama; Koichi Wakabayashi; Hidenao Sasaki
  Scientific reports, 8, 1, 819, 819, 2018年01月16日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, Clinical diagnosis of progressive supranuclear palsy (PSP) is sometimes difficult because various phenotypes have been identified. Here, we report a mutation in the bassoon (BSN) gene in a family with PSP-like syndrome. Their clinical features resembled not only those of PSP patients but also those of individuals with multiple system atrophy and Alzheimer's disease. The neuropathological findings showed a novel three + four repeat tauopathy with pallido-luysio-nigral degeneration and hippocampal sclerosis. Whole-exome analysis of this family identified a novel missense mutation in BSN. Within the pedigree, the detected BSN mutation was found only in affected individuals. Further genetic analyses were conducted in probands from four other pedigrees with PSP-like syndrome and in 41 sporadic cases. Three missense mutations in BSN that are very rarely listed in databases of healthy subjects were found in four sporadic cases. Western blot analysis of tau following the overexpression of wild-type or mutated BSN revealed the possibility that wild-type BSN reduced tau accumulation, while mutated BSN lost this function. An association between BSN and neurological diseases has not been previously reported. Our results revealed that the neurodegenerative disorder associated with the original proband's pedigree is a novel tauopathy, differing from known dementia and parkinsonism syndromes, including PSP.
• miR-23a promotes invasion of glioblastoma via HOXD10-regulated glial-mesenchymal transition.
  Kazuhiro Yachi; Masumi Tsuda; Shinji Kohsaka; Lei Wang; Yoshitaka Oda; Satoshi Tanikawa; Yusuke Ohba; Shinya Tanaka
  Signal transduction and targeted therapy, 3, 33, 33, 33, 2018年, ［査読有り］, ［国際誌］
  英語, Glioblastoma is the most aggressive and invasive brain tumor and has a poor prognosis; elucidating the underlying molecular mechanisms is essential to select molecular targeted therapies. Here, we investigated the effect of microRNAs on the marked invasiveness of glioblastoma. U373 glioblastoma cells were infected with 140 different microRNAs from an OncomiR library, and the effects of the invasion-related microRNAs and targeted molecules were investigated after repeated Matrigel invasion assays. Screening of the OncomiR library identified miR-23a as a key regulator of glioblastoma invasion. In six glioblastoma cell lines, a positive correlation was detected between the expression levels of miR-23a and invasiveness. A luciferase reporter assay demonstrated that homeobox D10 (HOXD10) was a miR-23a-target molecule, which was verified by high scores from both the PicTar and miRanda algorithms. Forced expression of miR-23a induced expression of invasion-related molecules, including uPAR, RhoA, and RhoC, and altered expression of glial-mesenchymal transition markers such as Snail, Slug, MMP2, MMP9, MMP14, and E-cadherin; however, these changes in expression levels were reversed by HOXD10 overexpression. Thus, miR-23a significantly promoted invasion of glioblastoma cells with polarized formation of focal adhesions, while exogenous HOXD10 overexpression reversed these phenomena. Here, we identify miR-23a-regulated HOXD10 as a pivotal regulator of invasion in glioblastoma, providing a novel mechanism for the aggressive invasiveness of this tumor and providing insight into potential therapeutic targets.
• A case of tumefactive multiple sclerosis resembling malignant glioma
  Kohei Ishikawa; Kenichi Sato; Tamio Ito; Yoshimaru Ozaki; Taku Asanome; Yohei Yamaguchi; Yuuki Ishida; Tomoaki Ishizuka; Naoyasu Okamura; Tomoki Fuchizaki; Satoshi Tanikawa; Shinya Tanaka; Hirohiko Nakamura
  Japanese Journal of Neurosurgery, 26, 9, 688, 694, Japanese Congress of Neurological Surgeons, 2017年
  日本語, 研究論文（学術雑誌）
• A case of giant cell-rich solitary fibrous tumor in the external auditory canal
  Sayaka Yuzawa; Satoshi Tanikawa; Isamu Kunibe; Hiroshi Nishihara; Kazuo Nagashima; Shinya Tanaka
  PATHOLOGY INTERNATIONAL, 66, 12, 701, 705, 2016年12月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Endosomal sorting related protein CHMP2B is localized in Lewy bodies and glial cytoplasmic inclusions in α-synucleinopathy
  Satoshi Tanikawa; Fumiaki Mori; Kunikazu Tanji; Akiyoshi Kakita; Hitoshi Takahashi; Koichi Wakabayashi
  Neuroscience letters, 527, 1, 16, 21, 2012年10月, ［査読有り］, ［筆頭著者］
  英語, 研究論文（学術雑誌）

• Artificial Intelligence Predicts the Genetic Information of The Integrated Diagnosis of Brain Tumors
  Yusuke Ishida; Masumi Tsuda; Jun Suzuka; Lei Wang; Satoshi Tanikawa; Hirokazu Sugino; Shinya Tanaka, MODERN PATHOLOGY, 32, 2019年03月
  英語, 研究発表ペーパー・要旨（国際会議）
• Artificial Intelligence Predicts the Genetic Information of The Integrated Diagnosis of Brain Tumors
  Yusuke Ishida; Masumi Tsuda; Jun Suzuka; Lei Wang; Satoshi Tanikawa; Hirokazu Sugino; Shinya Tanaka, LABORATORY INVESTIGATION, 99, 2019年03月
  英語, 研究発表ペーパー・要旨（国際会議）
• A case of cauda equina primary extramedullary plasmacytoma
  Yoshitaka Oda; Hirokazu Sugino; Izumi Koyanagi; Tanikawa Satoshi; Yuusuke Ishida; Masumi Tsuda; Shinya Tanaka, BRAIN PATHOLOGY, 29, 165, 165, 2019年02月
  英語, 研究発表ペーパー・要旨（国際会議）
• Development of bipolar charged hydrogel for neuronal tissue engineering
  Satoshi Tanikawa; Shingo Semba; Lei Wang; Mishie Tanino; Yusuke Ishida; Hirokazu Sugino; Jun Suzuka; Masumi Tsuda; Shinya Tanaka, BRAIN PATHOLOGY, 29, 109, 109, 2019年02月
  英語, 研究発表ペーパー・要旨（国際会議）
• てんかん様発作と脳炎様症状を繰り返した神経核内封入体病の1例
  大嶌祐貴; 工藤彰彦; 工藤彰彦; 水島慶一; 芳野正修; 佐藤智彦; 長沼亮滋; 上床尚; 白井慎一; 高橋育子; 松島理明; 谷川聖; 矢部一; 佐々木秀直, 臨床神経学(Web), 59, 1, 2019年
• てんかん様発作と脳炎様症状を繰り返した神経核内封入体病の1例
  大嶌 祐貴; 工藤 彰彦; 水島 慶一; 芳野 正修; 佐藤 智香; 長沼 亮滋; 上床 尚; 白井 慎一; 高橋 育子; 松島 理明; 谷川 聖; 矢部 一郎; 佐々木 秀直, 臨床神経学, 59, 1, 50, 50, 2019年01月
  (一社)日本神経学会, 日本語
• 家族性進行性核上性麻痺の遺伝子解析に基づいた孤発性進行性核上性麻痺の遺伝子解析
  矢部 一郎; 加藤 容崇; 谷川 聖; 三木 康生; 白井 慎一; 高橋 育子; 矢口 裕章; 藤岡 伸助; 國枝 保幸; 西原 広史; 田中 伸哉; 坪井 義夫; 若林 孝一; 佐々木 秀直, 臨床神経学, 56, Suppl., S317, S317, 2016年12月
  (一社)日本神経学会, 日本語

• 日本病理学会
• 日本神経病理学会

• 多孔性高機能ハイドロゲルを用いたin vitro3D神経膠芽腫組織の創出
  科学研究費助成事業
  2021年04月01日 - 2024年03月31日
  王 磊; 谷川 聖
  悪性脳腫瘍（神経膠芽腫Glioblastoma, GBM）は治療抵抗性が高く、再発が多く、5年生存率は8%以下と予後が極めて悪い。治療抵抗性と再発はGBMのがん幹細胞（GBM stem cell, GSC）が再増殖するため、GSCの性質を知り、特異的に標的とする治療法の開発が必要である。しかしながら、GSCはがん組織中に微量であり、症例ごとにマーカーも異なり、同一組織の中で多様性があるため研究論文は多数あるが、実臨床での治療標的には至っていない。申請者らは、北大オリジナル高機能高分子ソフト＆ウェットマター（以下、高機能ハイドロゲル）を用いて高速・効率的ながん幹細胞の誘導法を開発した。本研究はこの誘導法を発展させて、Porous化した高機能ハイドロゲルを用いて、GSCニッシェを創出することで、ヒトの脳内病変を模倣したGBM組織を再構築する。そしてニッシェがGSCを制御・維持するしくみを解明し、それに基づくGSCに特異的な治療法の開発を目指す。昨年度血管内皮及び周皮細胞を用いた血管網の構築に最適なPorous高機能ハイドロゲルの創出について：ゲルの物理特性として、力学的性質および電荷密度の2点に着目した。力学的にハイドロゲルは生体軟組織と同程度の弾性率を有し、弾性率は調製可能なため、引張試験・動的粘弾性試験により培養基盤の粘弾性的性質を定量化した。物理因子を調節することで目的に血管内皮及び周皮細胞に対して最適な高機能ゲルを作成した。HEMA濃度の調整で、最適なporeサイズ（5~20μm）を探し出した。血管内皮及び周皮細胞に対して最適な培養する高機能ゲルを見出した。また、数種類の神経膠腫培養細胞株を高機能ハイドロゲル上で培養すると幹細胞様sphereを形成し、幹細胞マーカーSox2、Oct3/4、Nanogが短時間で発現亢進することを見出した。これら細胞を用いて、血管内皮及び周皮細胞を導入したPorous化高機能ハイドロゲル上の3D培養を試みる予定です。
  日本学術振興会, 基盤研究(C), 北海道大学, 21K12679
• 粒子化ハイドロゲルを用いた血管網を有する脳組織の構築
  科学研究費助成事業
  2019年 - 2022年
  谷川 聖
  日本学術振興会, 研究代表者, 競争的資金