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中島 千絵 (ナカジマ チエ)
| 人獣共通感染症国際共同研究所 バイオリソース部門 | 教授 |
| 獣医学研究院 | 教授 |
| 総合イノベーション創発機構ワクチン研究開発拠点 | 教授 |
| Ｏｎｅ Ｈｅａｌｔｈリサーチセンター | 教授 |
研究活動情報
■ 論文- Application of multiplex PCR-based nanopore sequencing for rapid detection and surveillance of antimicrobial-resistant Mycoplasma bovis in Japanese dairy cattle
Masaru Usui; Masashi Ebisawa; Shingo Okamura; Azul Noda; Akira Fukuda; Chie Nakajima; Yasuhiko Suzuki
Microbiology Spectrum, 2026年08月04日
研究論文（学術雑誌） - Genomic comparison of clinical strains of Mycobacterium shinjukuense in Japan reveals low diversity and stable genome structures.
Takayuki Wada; Hiharu Inoue; Shiomi Yoshida; Yoshiro Murase; Yuriko Igarashi; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Satoshi Mitarai
Microbial genomics, 12, 5, 2026年05月, ［国際誌］
英語, 研究論文（学術雑誌）, Mycobacterium shinjukuense, a rare non-tuberculous mycobacterial species closely related to Mycobacterium tuberculosis, remains poorly characterized at the genomic level. To obtain insights into its intraspecies genomic diversity and structure variation, we performed whole-genome sequencing on 18 clinical strains of M. shinjukuense collected from Japan between 2010 and 2017. Phylogenetic analysis revealed limited overall genetic diversity, with several clonal clusters likely representing identical strains. The phylogeny exhibited a largely star-like topology with few single nucleotide variations accumulated over time, suggesting a clonal expansion. Complete genome sequencing of five representative strains revealed high synteny, with no large-scale rearrangements, suggesting stable genomic structures. One strain, MSJ-01, harboured a unique 30 kb insertion region, possibly acquired via horizontal gene transfer. These findings provide insights into the genomic diversity and structure of M. shinjukuense, suggesting both its evolutionary stability and its potential to incorporate foreign DNA. This work expands upon available genomic resources and supports the utility of M. shinjukuense as a model for understanding genome evolution in the M. tuberculosis-associated phylotype lineage. - Development of a Novel Real-Time PCR Assay Differentiating Burkholderia mallei From Burkholderia pseudomallei.
Ai Koshikawa; Yoshiki Ichikawa; Mitsuru Nakase; Apichai Tuanyok; Vannarat Saechan; Keisuke Aoshima; Hideaki Higashi; Chie Nakajima; Yasuhiko Suzuki; Vanaabaatar Batbaatar; Takashi Kimura
Microbiology and immunology, 2026年04月29日, ［国際誌］
英語, 研究論文（学術雑誌）, Glanders is a zoonotic disease caused by Burkholderia mallei (Bm). Bm is phylogenetically similar to B. pseudomallei (Bp), the causative agent of melioidosis, making it difficult to differentiate between them. Recently, the PCR method recommended by the World Organisation for Animal Health (WOAH) failed to detect a Kuwait strain of Bm, and the WOAH has cautioned that ongoing Bm evolution may lead to the emergence of variants that escape standard assays. Therefore, this study aimed to develop a novel real-time PCR method to discriminate between Bm and Bp. Local and web-based BLAST searches identified a 494-bp sequence conserved across Bm but absent from other bacterial genomes, including Bp, except for one B. thailandensis strain. The optimized probe-based real-time PCR targeting this sequence detected the genomes of five Bm isolates but did not amplify DNA from closely related Burkholderia species. In clinical specimens from Mongolia, this assay amplified Bm DNA at an equivalent detection rate to the WOAH-recommended real-time PCR method. These findings provide a novel, highly sensitive diagnostic method for differentiating glanders from melioidosis and suggest its potential use as an alternative method in clinical applications. Further verification using a large number of samples collected from various regions will be necessary in the future. - パンデミック型E.coliクローンにおけるコリスチン耐性の健康影響のin vivo再評価(In vivo reassessment of the health impact of colistin resistance in a pandemic E. coli clone)
トイティン ジラッチャヤ[平石]; 佐藤 豊孝; 山本 聡; 遠山 茉奈; 臼井 優; 小笠原 徳子; 林 航; 長野 則之; 中島 千絵; 鈴木 定彦; 堀内 基広; 高橋 聡; 横田 伸一; 田村 豊
日本細菌学雑誌, 81, 1, 39, 39, 日本細菌学会, 2026年03月
英語 - Antimicrobial susceptibility of Escherichia coli isolated from wild deer (Cervus nippon yesoensis) and fox (Vulpes vulpes schrencki) in Hokkaido: a pilot study on sustainable antimicrobial resistance monitoring.
Yui Kawabata; Akira Fukuda; Rin Sato; Hirokazu Kouguchi; Chie Nakajima; Yasuhiko Suzuki; Kohei Makita; Tetsuo Asai; Masaru Usui
The Journal of veterinary medical science, 2026年02月27日, ［国内誌］
英語, 研究論文（学術雑誌）, In Japan, sustainable national antimicrobial resistance (AMR) monitoring has been established in both human and veterinary fields. However, AMR monitoring in the environment, including wildlife, remains limited. This study aimed to assess the possibility of sustainable monitoring using wild deer transported to slaughterhouses and wild foxes surveyed for Echinococcus multilocularis. Escherichia coli, a common AMR indicator bacterium, was isolated from rectal swabs of deer and foxes using Chromagar ECC, with and without antimicrobials (cefotaxime (CTX) or nalidixic acid (NA). Antimicrobial susceptibility was determined, and β-lactamase genes were detected using polymerase chain reaction. Plasmids carrying β-lactamase genes were analyzed by whole-genome sequencing. A total of 104 E. coli strains were isolated from (103/120, 85.8%) deer and 61 strains from (56/99, 56.6%) foxes using medium without antimicrobials. Using a medium containing CTX and NA, two and three strains were isolated, respectively, exclusively from foxes. All deer strains were susceptible to the tested antimicrobials. However, 0-11.5% of fox strains from medium without antimicrobials (n=61) demonstrated resistance. Three strains from foxes had β-lactamase genes in plasmid. Finally, this study demonstrates the feasibility of sustainable AMR monitoring using wild deer transported to a slaughterhouse and wild foxes surveyed for E. multilocularis. - Antimicrobial resistance, extended-spectrum β-lactamase determinants, and virulence gene profiles of Escherichia coli along the pork production chain in central Thailand
Watsawan Prapasawat; Achiraya Siriphap; Sirikarn Wiriyasirivaj; Apiradee Intarapuk; Ruttana Pachanon; Chie Nakajima; Yasuhiko Suzuki; Orasa Suthienkul
Veterinary World, 2026年01月31日
研究論文（学術雑誌） - Insight into the genetic diversity of Mycobacterium bovis isolated from cattle in Ghana.
David Atomanyi Barnes; Nathan Grant-Biney; Philip Tetteh; Emelia Konadu Danso; Prince Asare; Jeewan Thapa; Tamsin S Barnes; Adwoa Asante-Poku; Samuel Adjei; Stephen V Gordon; Yasuhiko Suzuki; Chie Nakajima
Microbiology spectrum, e0213325, 2026年01月27日, ［国際誌］
英語, 研究論文（学術雑誌）, Bovine tuberculosis (bTB), caused primarily by Mycobacterium bovis, poses significant risks to cattle and public health in Ghana. Previous studies reported varying prevalence (3%-21%) and M. bovis strain distributions, necessitating a deeper understanding of the molecular epidemiology of bTB to guide control strategies. This study investigated the genetic diversity and phylogenetic relationships of M. bovis isolates from cattle in Ghanaian abattoirs and compared results with strains from other African countries. Spoligotyping analysis of 28 M. bovis isolates confirmed 6 distinct spoligotypes, with SB0944 being the most prevalent (64.3%). Mycobacterial Interspersed Repetitive Units-Variable Number Tandem Repeats (MIRU-VNTR) genotyping analysis further resolved the isolates into 22 genotypes, with loci MIRU26, QUB26, and ETR-A showing the highest allelic diversity. Mixed infections were found in 10% of culture-confirmed cases, indicating a high risk of co-infection with multiple strains in the area. Comparative analysis revealed that Ghanaian isolates clustered within the African 1 (Af1) clonal complex, consistent with strains from Nigeria, Burkina Faso, and Cameroon, but distinct from the BCG-like and other strains that were dominant in Algeria and, to some extent, in Mali. Minimal strain homogenization of West-Central Africa with other regions suggests localized evolution, shaped by ecological barriers, regulated transhumance, and possible competitive exclusion among strains. These findings provide evidence for the need for region-specific bTB control measures that reflect Africa's unique livestock dynamics and micro-epidemic patterns. Enhanced surveillance with advanced genotyping methods could improve outbreak tracking and inform targeted interventions to mitigate bTB's impact on livestock in this region.IMPORTANCEBovine tuberculosis (bTB), caused primarily by Mycobacterium bovis, is a neglected disease in many low- and middle-income countries, including Ghana. Our study reveals that while M. bovis strains in Ghana show high local genetic diversity, they belong almost exclusively to a single clonal complex (Af1) shared across West-Central Africa. Despite regional regulations governing transhumance and cattle movement, the observed strain mixing suggests an "island-like" evolutionary structure for M. bovis in this area; that is, diverse within West-Central African countries but genetically isolated. This pattern is shaped by natural barriers, such as deserts and forests, which restrict strain flow while permitting intense intra-regional transmission. Understanding M. bovis evolution in this context is essential for tailoring effective, region-specific interventions. Our findings highlight the need to treat West-Central Africa as a community of interconnected but genetically distinct bTB zones, calling for both local action and coordinated regional strategies to mitigate bTB. - Bacterial contamination level and characterization of antimicrobial-resistant bacteria in commercial pet foods in Japan.
Akira Fukuda; Kazu Yamaura; Kazuyoshi Tokumoto; Yasuhiko Suzuki; Chie Nakajima; Shoichiro Yukawa; Masaru Usui
One health (Amsterdam, Netherlands), 21, 101197, 101197, 2025年12月, ［国際誌］
英語, 研究論文（学術雑誌）, With the growing pet market, various types of pet food become available. Pet foods, especially raw meat-based diets (RMBDs), are contaminated with pathogens and antimicrobial-resistant bacteria, leading to health concerns for pets and humans. The bacterial contamination levels in pet food, including the presence of antimicrobial-resistant bacteria, have not been extensively studied in Japan. To address this issue in commercial pet foods in Japan, we evaluated the standard plate counts (SPCs) of various pet food samples, and isolated and characterized Escherichia coli and Enterococcus spp. A total of 129 pet foods (48 RMBDs, 21 heat-treated foods, and 60 treats) were purchased, and SPC quantification and isolation of E. coli and Enterococcus spp. were performed. SPCs in RMBD were significantly higher than in heat-treated foods and treats. Specifically, 50.0 % of the RMBD samples had SPCs exceeding 106 CFU/g. E. coli was isolated only from 62.5 % of RMBDs. Four cephalosporin-resistant E. coli strains harboring bla genes were detected, among which, one carried mcr conferring resistance to colistin. Enterococcus spp. were isolated from 89.6 % of RMBDs and 23.3 % of treats. Linezolid-non-susceptible E. faecalis harboring optrA or poxtA genes were detected in four RMBDs. Pet food may be contaminated with bacteria; some RMBDs are contaminated at concentrations >106 CFU/g, and with veterinary and human clinically important antimicrobial-resistant bacteria. To prevent health risks in both humans and pets associated with pet food, ensuring the hygienic management of pet food, especially RMBDs, and promoting accurate knowledge among pet owners are important. - Diagnostic Stewardship Trends and Antimicrobial Resistance Profiles of Bacteria Isolated in Zambia: A Five-Year Retrospective Study (2020-2024).
Joseph Yamweka Chizimu; Aubrey Chichonyi Kalungia; Steward Mudenda; Zoran Muhimba; Chileshe Lukwesa; Webrod Mufwambi; Amon Siame; Kelvin Mwangilwa; Priscilla Gardner; Jimmy Hangoma; Victor Daka; Chikwanda Chileshe; Ntombi B Mudenda; Maisa Kasanga; Misheck Shawa; Herman Chambaro; Duncan Chanda; Taona Sinyawa; Bertha Chibwe; Kaunda Kaunda; Kaunda Yamba; O-Tipo Shikanga; Freddie Masaninga; Mpela Chibi; Sandra Diana Mwadetsa; Doreen Mainza Shempela; Mwendalubi Albert Hadunka; Samson Mukale; Andrew Bambala; Malambo Mutila; Loveness Sakalimbwe; Fred Mulako Simwinji; Geoffrey Mainda; Chie Nakajima; Ruth Nakazwe; Fusya Goma; Nyambe Sinyange; Yasuhiko Suzuki; John Bwalya Muma; Roma Chilengi
Antibiotics (Basel, Switzerland), 14, 11, 2025年11月10日, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND/OBJECTIVES: The right diagnostic tests, for the right patient, at the right time, are key to optimising antimicrobial use (AMU) and preventing antimicrobial resistance (AMR). This study evaluated diagnostic stewardship trends and AMR patterns in Zambian surveillance sentinel sites from 2020 to 2024. METHODS: This descriptive, retrospective study analysed routine laboratory data from January 2020 to December 2024 at seven designated AMR surveillance sentinel sites across Zambia. Data on clinical specimens submitted for antimicrobial susceptibility testing were extracted from WHONET and analysed by year, specimen type, and antimicrobial susceptibility profile. RESULTS: A total of 184,788 bacteriology specimens were processed over five years. Urine was the most frequently collected specimen, peaking above 20,000 in 2024. Escherichia coli was the most commonly isolated organism among 15 priority pathogens, comprising 25.9% of the 30,013 isolates. Though a statistically significant increasing trend in total organism isolations was observed annually, only Shigella sp. demonstrated a substantial increase in non-susceptibility to azithromycin (p = 0.027). High resistance was observed with doxycycline, azithromycin, clindamycin, trimethoprim/sulfamethoxazole, ciprofloxacin, and rifampicin, exhibiting resistance ranging from 50% to 80%. Critical AMR alerts included 65% for Vancomycin-Resistant Enterococcus (VRE), 72% for linezolid, and 44% for carbapenems, and possible ESBL-producing Enterobacterales showing the highest overall resistance at 35%, across sentinel sites. CONCLUSIONS: Zambia faces a concerning and significant increase in AMR, with high resistance prevalence across commonly used antibiotics. Critical resistance alerts for VRE, linezolid, carbapenems, and possible ESBL-producing Enterobacterales underscore an urgent need for robust antimicrobial stewardship and continuous diagnostic surveillance. - Impact of Multidisciplinary-Led Implementation of Antimicrobial Stewardship Programs in Zambia: Findings and Implications.
Joseph Yamweka Chizimu; Steward Mudenda; Victor Daka; Webrod Mufwambi; Zoran Muhimba; Kaunda Yamba; Misheck Shawa; Kelvin Mwangilwa; Jimmy Hangoma; Sombo Fwoloshi; Amon Siame; Kaunda Kaunda; Andrew Bambala; Kenneth Kapolowe; Priscilla Nkonde Gardner; Duncan Chanda; Shempela Doreen; Charles Chileshe; Paul Simujayang Ombe; Ntombi B Mudenda; Loveness Sakalimbwe; Aubrey C Kalungia; Chikwanda Chileshe; Taona Sinyawa; Maisa Kasanga; Raphael Chanda; Samson Mukale; Shikanga O-Tipo; Evelyn Wesangula; Cephas Sialubanje; Adrian Muwonge; Fred Simwinji; Chie Nakajima; Freddie Masaninga; Fusya Goma; Nyambe Sinyange; Yasuhiko Suzuki; John Bwalya Muma; Roma Chilengi
Antibiotics (Basel, Switzerland), 14, 11, 2025年11月07日, ［国際誌］
英語, 研究論文（学術雑誌）, Background/Objectives: Antimicrobial resistance (AMR) is a critical global health threat, with sub-Saharan Africa disproportionately affected. Antimicrobial stewardship (AMS) programs are essential in combating AMR; however, data on their implementation in resource-limited settings like Zambia remain scarce. This study assessed the post-implementation status of World Health Organization (WHO) AMS core elements in selected public hospitals in Zambia. Methods: A cross-sectional pre- and post-intervention survey was conducted in 11 public hospitals across Zambia's 10 provinces. Baseline (pre-implementation) and 12-month follow-up (post-implementation) assessments were carried out using the WHO-adapted Periodic National and Healthcare Facility Assessment Tool. The six AMS core elements evaluated included leadership, accountability, AMS actions, education, monitoring, and feedback. Results: The average AMS program score increased from 59% at pre-implementation to 81% at post-implementation. Significant improvements were observed in education and training (+36%) and accountability (+31%). While leadership and monitoring also showed positive trends, gaps persisted in AMS actions (63%) and feedback/reporting mechanisms (68%). Drug and Therapeutics Committee (DTC) functionality improved by 23%, with 90% of facilities now holding regular DTC meetings. Implementation of AMS actions, such as ward rounds, rose from 0% to 73%. Challenges remained in clinical audit feedback, resource mobilization, and prescribing optimization. Variability across facilities highlighted differences in leadership, resources, and technical capacity. Conclusions: AMS implementation in Zambia improved substantially across key domains. However, sustained leadership, adequate financing, and continuous capacity-building are needed to address persistent gaps and ensure long-term success in mitigating AMR. - Simultaneous Detection of Fluoroquinolone- and Macrolide-Resistant Campylobacter jejuni/Campylobacter coli in Retail Chicken Meat Using Multiplex-PCR.
Akira Fukuda; Ayu Matsui; Yuma Furuya; Yasuhiko Suzuki; Yutaka Tamura; Chie Nakajima; Masaru Usui
Microbial drug resistance (Larchmont, N.Y.), 2025年10月20日, ［国際誌］
英語, 研究論文（学術雑誌）, Campylobacter contamination in chicken meat, particularly with fluoroquinolone- and macrolide-resistant strains, is a significant public health concern. Therefore, developing an easy and rapid detection method for fluoroquinolone- and macrolide-resistant Campylobacter will improve food safety. In this study, we designed and evaluated a multiplex-Polymerase Chain Reaction (PCR) method for the simultaneous detection of fluoroquinolone- and macrolide-resistant Campylobacter jejuni and Campylobacter coli in chicken meat. Our multiplex-PCR-targeted mutations in the gyrA and rrl genes of Campylobacter species confer resistance to fluoroquinolones and macrolides, respectively. Overall, this method could detect Campylobacter contamination and identify C. jejuni and C. coli as well as their susceptibility to fluoroquinolone and macrolide. Spiking of chicken meat samples revealed that multiplex-PCR can detect at least 0.72 Colony-Forming Unit (CFU)/g C. jejuni/coli. Among 277 retail chicken meat samples, C. jejuni/coli were detected in 54 samples using multiplex-PCR, with isolation from 52 samples using microbial culture. The susceptibility of the isolates to fluoroquinolones and macrolides was determined using both the PCR and culture methods, and a comparison of these methods showed that the accuracy, specificity, and sensitivity were all above 95%. The culture method required approximately 8 days for Campylobacter isolation and antimicrobial susceptibility testing. Multiplex-PCR could determine the contamination with C. jejuni/coli and susceptibility to fluoroquinolones and macrolides within 2-3 days from the enrichment culture to final determination. These results indicate that the proposed multiplex-PCR enables rapid and simultaneous detection of fluoroquinolone- and macrolide-resistant C. jejuni/coli in chicken meat and its utility for monitoring their contamination in food. - イヌ常在菌Staphylococcus pseudintermedius由来SpsQとイヌIgGとの結合性の評価
竹内 寛人; 中島 千絵; 今内 覚; 前川 直也; 岡川 朋弘; 臼井 優; 田村 豊; 鈴木 定彦; 村田 史郎; 大橋 和彦
日本獣医学会学術集会講演要旨集, 168回, 208, 208, (公社)日本獣医学会, 2025年09月
日本語 - Complete genome sequence of Treponema medium isolated from foot of bovine digital dermatitis in Japan
Akira Fukuda; Takashi Murakami; Noritsugu Abe; Yasuhiko Suzuki; Chie Nakajima; Masaru Usui
Microbiology Resource Announcements, 14, 10, e0065625, 2025年08月27日, ［国際誌］
英語, 研究論文（学術雑誌）, Treponema species are the primary causative bacteria of bovine digital dermatitis. This report describes the isolation and complete genome sequencing of Treponema medium strain T1 isolated from a hoof sole swab of a bovine in Japan. Genome sequences were obtained using a combination of long- and short-read sequencing technologies. - Emergence of livestock-associated MRSA clonal complex 398 in Thailand's swine industry.
Pawarut Narongpun; Pattrarat Chanchaithong; Wimonrat Tanomsridachchai; Narong Nuanmuang; Jeewan Thapa; Junya Yamagishi; Orasa Suthienkul; Pimlapas Leekitcharoenphon; Frank M Aarestrup; Chie Nakajima; Yasuhiko Suzuki
The Journal of antimicrobial chemotherapy, 2025年08月26日, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Livestock-associated MRSA (LA-MRSA) clonal complex (CC) 398 is primarily found among pigs across Europe. However, CC398 has also been sporadically identified in several Asian countries, including Thailand. This study aimed to examine the drivers contributing to the emergence of LA-MRSA CC398 in Thailand's swine industry. METHODS: Whole-genome sequencing data of 18 LA-MRSA CC398 isolates from pigs, retail pork and swine workers in Thailand were analysed. Additionally, a total of 1197 qualified genomes from various sources in 24 countries were retrieved from Pathogenwatch. Subsequently, two SNP-based phylogenetic trees were reconstructed using maximum likelihood (n = 1215) and Bayesian inference (n = 168). RESULTS: Our findings revealed that Staphylococcus aureus isolates with the CC398-SCCmec Vc (5C2&5)-t034 genotype were closely related to European isolates, including those from Denmark. In contrast, S. aureus isolates belonging to the CC398-composite SCCmec-t034 formed a phylogenetically distinct cluster. CONCLUSIONS: This study reports that the CC398-SCCmec Vc (5C2&5)-t034 genotype identified in Thai pigs may have originated from Danish swine populations, possibly introduced into Thailand through international livestock movement. The CC398-composite SCCmec-t034 genotype was also detected in domestic pigs; however, it appears to have evolved independently from the CC398-SCCmec Vc (5C2&5)-t034 genotype. Bayesian phylogenetic analysis estimates that the common ancestor of the composite genotype emerged earlier than that of the CC398-SCCmec Vc (5C2&5)-t034 genotype, indicating a separate evolutionary origin. - Genomic insights into ceftazidime resistance in Burkholderia pseudomallei: discovery of A172T mutation and palindromic GC-rich repeat sequences facilitating penA duplication and amplification.
Apichai Tuanyok; Chie Nakajima; Tiernan Noll; Md Siddiqur Rahman Khan; Pacharapong Khrongsee; Charles A Yowell; Yu-Ping Xiao; Vanaporn Wuthiekanun; Narisara Chantratita; Henry Heine; Kuttichantran Subramaniam; Yasuhiko Suzuki; Direk Limmathurotsakul; Ayalew Mergia
Antimicrobial agents and chemotherapy, 69, 8, e0022025, 2025年08月06日, ［国際誌］
英語, 研究論文（学術雑誌）, Ceftazidime (CAZ) resistance in Burkholderia pseudomallei, the causative agent of melioidosis, complicates treatment in endemic regions. This study identified a novel A172T mutation and other known penA mutations as critical contributors to CAZ resistance in a large Thai strain collection. Frequent gene duplication and amplification of penA, likely driven by Palindromic GC-Rich Repeat Sequences, highlights the urgent need for rapid diagnostics and optimized treatment strategies to manage this life-threatening disease effectively. - Unveiling the Genetic Diversity and Antimicrobial Resistance Profiles of Salmonella Population From 2016 to 2020 in Thai Canal Water.
Jirachaya Toyting-Hiraishi; Toyotaka Sato; Neunghatai Supha; Yuwanda Thongpanich; Motohiro Horiuchi; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki; Fuangfa Utrarachkij
Environmental microbiology reports, 17, 4, e70160, 2025年08月, ［国際誌］
英語, 研究論文（学術雑誌）, Salmonella is one of the important pathogens causing acute gastroenteritis, and antimicrobial-resistant Salmonella raises a critical public health concern. Canals in Bangkok, Thailand, play a vital role as sources of agricultural and daily water usage. By employing whole genome sequencing to analyse 351 Salmonella genomes isolated between 2016 and 2020, we expanded the understanding of the characteristics and antimicrobial resistance properties of Salmonella enterica found in Bangkok canals, an underrepresented biome in research. Salmonella Agona was the dominant serotype, while S. Typhimurium and its monophasic variant were periodically found. Seven new sequence types (STs) were identified, including STs 11,346, 11,347, 11,348, 11,349, 11,350, 11,351, and 11,352. Seven chromosomal-mediated gene mutations and 50 antimicrobial resistance genes were detected. The three most common resistance genes were tet(A), blaTEM-1B, and qnrS1. The tet(X4) was first identified in the Salmonella population in Thailand, and mcr-3.1 was also detected. In total, 39.0% of the strains were potentially multidrug-resistant. The strains carried double amino acid substitutions in GyrA and ParC, and a strain with GyrA substitutions and qnrS1 exhibited the strongest resistance to nalidixic acid and ciprofloxacin. Most of the ceftazidime, ceftriaxone, and cefotaxime-resistant strains (66.7%) harboured blaCTX-M-55. Col(pHAD28) was the predominant plasmid replicon type. Phylogenetic analysis of Salmonella STs 34 and 213 from canal water and the strains from databases showed the possibility of circulation of STs 34 and 213 between canal water and humans in Thailand and worldwide. These findings shed light on the circulation of antimicrobial-resistant pathogens in the environmental water and advocate for incorporating environmental sampling into comprehensive AMR surveillance programmes within a One Health framework. - Investigation of WQ-3810, a Fluoroquinolone with a High Potential Against Fluoroquinolone-Resistant Mycobacterium avium.
Sasini Jayaweera; Pondpan Suwanthada; David Atomanyi Barnes; Charlotte Poussier; Tomoyasu Nishimura; Naoki Hasegawa; Yukiko Nishiuchi; Jeewan Thapa; Stephen V Gordon; Hyun Kim; Chie Nakajima; Yasuhiko Suzuki
Antibiotics (Basel, Switzerland), 14, 7, 2025年07月14日, ［国際誌］
英語, 研究論文（学術雑誌）, Background/Objectives:Mycobacterium avium, a member of Mycobacterium avium complex (MAC), is an emerging opportunistic pathogen causing MAC-pulmonary disease (PD). Fluoroquinolones (FQs), along with ethambutol (EMB) and rifampicin, are recommended for macrolide-resistant MAC-PD; however, FQ-resistant M. avium have been reported worldwide. WQ-3810 is an FQ with high potency against FQ-resistant pathogens; however, its activity against M. avium has not yet been studied. Methods: In this study, we conducted a DNA supercoiling inhibitory assay to evaluate the inhibitory effect of WQ-3810 on recombinant wild-type (WT) and four mutant DNA gyrases of M. avium and compared the IC50s of WQ-3810 with those of ciprofloxacin (CIP), levofloxacin (LVX), and moxifloxacin (MXF). In addition, we examined WQ-3810's antimicrobial activity against 11 M. avium clinical isolates, including FQ-resistant isolates, with that of other FQs. Furthermore, we assessed the synergistic action of WQ-3810 with the combination of either EMB or isoniazid (INH). Results: In a DNA supercoiling inhibitory assay, WQ-3810 showed 1.8 to 13.7-fold higher efficacy than LVX and CIP. In the MIC assay, WQ-3810 showed 4 to 8-fold, 2 to 16-fold, and 2 to 4-fold higher antimicrobial activity against FQ-resistant isolates than CIP, LVX, and MXF, respectively. The combination of WQ-3810 and INH exhibited a synergistic relationship. Conclusions: The overall characteristics of WQ-3810 demonstrated greater effectiveness than three other FQs, suggesting that it is a promising option for treating FQ-resistant M. avium infections. - Development of a multiplex loop-mediated isothermal amplification (LAMP) method for differential detection of Mycobacterium bovis and Mycobacterium tuberculosis by dipstick DNA chromatography.
Mwangala L Akapelwa; Thoko F Kapalamula; Lavel C Moonga; Precious Bwalya; Eddie S Solo; Joseph Y Chizimu; Jeewan Thapa; Kyoko Hayashida; Bernard M Hang'ombe; Musso Munyeme; Aki Tamaru; Takayuki Wada; Shiomi Yoshida; Takuya Kodera; Mistuo Kawase; Stephen V Gordon; Keiko Yamada; Chie Nakajima; Yasuhiko Suzuki
Microbiology spectrum, 13, 6, e0242124, 2025年06月03日, ［国際誌］
英語, 研究論文（学術雑誌）, UNLABELLED: Although human tuberculosis (TB) caused by Mycobacterium bovis is clinically, pathologically, and radiologically indistinguishable from Mycobacterium tuberculosis-caused TB, M. bovis is innately resistant to pyrazinamide, a key first-line drug effective against M. tuberculosis. The rapid differentiation of these two biovars is therefore of high clinical and epidemiologic importance. Most current molecular tools in resource-limited settings identify mycobacteria only to the M. tuberculosis species (MTB) level. In this study, we report a multiplex loop-mediated isothermal amplification (LAMP) method coupled with dipstick chromatography for the rapid and easy differential detection of M. bovis and M. tuberculosis. The assay was optimized and validated using 143 isolates comprising six MTB reference strains, 50 M. bovis isolates, 58 M. tuberculosis isolates, 24 non-tuberculous mycobacterial (NTM) strains, and five other respiratory pathogens. The multiplex LAMP correctly detected MTB and distinguished between M. tuberculosis and M. bovis simultaneously with sensitivities of 500 fg and 1 pg DNA, respectively, within 60 min, and the results were visualized by dipstick chromatography within 10 min. The assay was specific in that no major respiratory pathogens tested, including NTM strains, were positive. The multiplex dipstick LAMP assay is therefore a useful and accurate low-cost method for the differential identification of M. bovis and M. tuberculosis, especially in endemic areas where bovine and human TB coexist. The distinction between M. bovis and M. tuberculosis can also aid in monitoring the spread of M. bovis to humans and allow for correct treatment, which will ultimately contribute to TB control in both humans and animals. IMPORTANCE: Human tuberculosis caused by Mycobacterium tuberculosis and Mycobacterium bovis shows similar clinical symptoms; however, the treatment differs because M. bovis is inherently resistant to pyrazinamide, a key first-line drug effective against M. tuberculosis. Most available molecular tools cannot distinguish the two biovars. This study addresses this gap by introducing a multiplex loop-mediated isothermal amplification (LAMP) method coupled with dipstick chromatography that can simultaneously and differentially detect M. bovis and M. tuberculosis within 60 min. The LAMP method does not require sophisticated high-cost equipment and can be easily implemented in resource-limited settings. Our LAMP facilitates rapid and accurate tuberculosis diagnosis, enabling appropriate therapeutic agents to be selected in areas where bovine and human tuberculosis coexist. It can also screen for M. bovis infection in humans and livestock, providing prevalence data in areas where such information is lacking. - Development of a PCR-dipstick DNA chromatography-based tool for the detection of CTX-M- and TEM-producing Escherichia coli and Klebsiella pneumoniae isolated from patients in Kafue and Katete districts of Zambia.
Misheck Shawa; Kyoko Hayashida; Naganori Nao; Atmika Paudel; Harvey Kamboyi; Herman Chambaro; Malala Mulavu; Cynthia Sipho Khumalo; Mike Nundwe; Tuvshinzaya Zorigt; Chinatsu Nakamura; Yongjin Qiu; Naoko Kawai; Maisa Kasanga; Joseph Chizimu; Lavel Moonga; Joseph Ndebe; Manyando Simbotwe; Shohei Ogata; Mulemba Samutela; Chie Nakajima; Roma Chilengi; Mable Mutengo; Masahiro Kajihara; Hirofumi Sawa; Bernard Hang'ombe; Yasuhiko Suzuki; Hideaki Higashi
BMC infectious diseases, 25, 1, 541, 541, 2025年04月16日, ［国際誌］
英語, 研究論文（学術雑誌）, In Zambia, 40% of clinical Gram-negative bacteria are either Escherichia coli or Klebsiella pneumoniae, with a high third-generation cephalosporin (3GC) resistance prevalence. Therefore, 3GC resistance surveillance is a crucial indicator for guiding focused intervention policies. However, the lack of genotypic diagnostic tools limits the ability to elucidate trends, especially in peri-urban and rural areas of developing countries. This study aimed to develop a rapid, cost-effective tool for the genotypic surveillance of 3GC resistance. Here, 900 stool samples collected from patients in Kafue (peri-urban, n = 400) and Katete (rural, n = 500) districts of Zambia were used for bacterial isolation on MacConkey agar supplemented with 1 μg/ml cefotaxime. Isolated 3GC-resistant strains were characterized by sequencing the 16S rRNA gene and screening for blaCTX-M and blaTEM genes using single polymerase chain reaction (PCR). Furthermore, selected 3GC-resistant strains were subjected to whole-genome sequencing (WGS) using MiSeq/HiSeq (n = 34) and MinION (n = 1). Using the data from this and other previous studies, we developed a rapid PCR-dipstick DNA chromatography-based tool for detecting blaCTX-M, blaTEM, E. coli-specific yaiO, and K. pneumoniae-specific khe genes. The prevalence of isolated 3GC resistant strains was 15.4% (139/900), dominated by E. coli (102/139, 73.4%). On PCR, the blaCTX-M gene was detected in 72.7% (101/139) of the isolates, while blaTEM was found in 46.8% (65/139) of the strains. The developed tool displayed a high level of agreement with WGS and single PCR/Sanger sequencing, with sensitivity and specificity ≥ 95% and Kappa ≥ 0.95 for each of the four target genes. We envisage that the simplicity and adaptability of this tool will be a significant advantage for the surveillance of 3GC resistance in Zambia and elsewhere. - 国境を越えて:留学生・ポスドクの挑戦 2016年から2020年までのタイの運河水におけるSalmonellaの遺伝的多様性と抗菌薬耐性(Beyond Borders: Challenges for International Students and Postdocs Genetic diversity and antimicrobial resistance of Salmonella in Thai canal water from 2016 to 2020)
Toyting Jirachaya; 佐藤 豊孝; Supha Neunghatai; Thongpanich Yuwanda; 堀内 基広; Thapa Jeewan; 中島 千絵; 鈴木 定彦; Utrarachkij Fuangfa
日本細菌学雑誌, 80, 2, 34, 34, 日本細菌学会, 2025年04月
英語 - Rapid and Integrated Bacterial Evolution Analysis unveils gene mutations and clinical risk of Klebsiella pneumoniae.
Kojiro Uemura; Toyotaka Sato; Soh Yamamoto; Noriko Ogasawara; Jirachaya Toyting; Kotaro Aoki; Akira Takasawa; Masayuki Koyama; Atsushi Saito; Takayuki Wada; Kaho Okada; Yurie Yoshida; Koji Kuronuma; Chie Nakajima; Yasuhiko Suzuki; Motohiro Horiuchi; Kenichi Takano; Satoshi Takahashi; Hirofumi Chiba; Shin-Ichi Yokota
Nature communications, 16, 1, 2917, 2917, 2025年03月25日, ［国際誌］
英語, 研究論文（学術雑誌）, Bacteria continually evolve. Previous studies have evaluated bacterial evolution in retrospect, but this approach is based on only speculation. Cohort studies are reliable but require a long duration. Additionally, identifying which genetic mutations that have emerged during bacterial evolution possess functions of interest to researchers is an exceptionally challenging task. Here, we establish a Rapid and Integrated Bacterial Evolution Analysis (RIBEA) based on serial passaging experiments using hypermutable strains, whole-genome and transposon-directed sequencing, and in vivo evaluations to monitor bacterial evolution in a cohort for one month. RIBEA reveals bacterial factors contributing to serum and antimicrobial resistance by identifying gene mutations that occurred during evolution in the major respiratory pathogen Klebsiella pneumoniae. RIBEA also enables the evaluation of the risk for the progression and the development of invasive ability from the lung to blood and antimicrobial resistance. Our results demonstrate that RIBEA enables the observation of bacterial evolution and the prediction and identification of clinically relevant high-risk bacterial strains, clarifying the associated pathogenicity and the development of antimicrobial resistance at genetic mutation level. - Antimicrobial susceptibility and genetic diversity of staphylococcus pseudintermedius isolated from companion animals and human clinical patients in Japan: Potential zoonotic implications.
Masaru Usui; Rana Fahmi Sabala; Sawa Morita; Akira Fukuda; Yuzo Tsuyuki; Kae Torii; Yuka Nakamura; Koichi Okamura; Tadato Komatsu; Junpei Sasaki; Chie Nakajima; Yasuhiko Suzuki
Journal of global antimicrobial resistance, 42, 66, 72, 2025年02月18日, ［国際誌］
英語, 研究論文（学術雑誌） - A novel ready-to-use loop-mediated isothermal amplification (LAMP) method for detection of Burkholderia mallei and B. pseudomallei.
Mitsuru Nakase; Jeewan Thapa; Vanaabaatar Batbaatar; Ochirbat Khurtsbaatar; Batchuluun Enkhtuul; Jugderkhorloo Unenbat; Baasansuren Lkham; Sachiho Fujita; Ai Koshikawa; Apichai Tuanyok; Vannarat Saechan; Hideaki Higashi; Kyoko Hayashida; Yasuhiko Suzuki; Chie Nakajima; Takashi Kimura
BMC microbiology, 25, 1, 36, 36, 2025年01月21日, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Glanders and melioidosis are contagious zoonotic diseases caused by Burkholderia mallei and B. pseudomallei, respectively. Bacterial isolation and polymerase chain reaction (PCR) have been used to detect these bacteria in animals suspected of infection; however, both methods require skilled experimental techniques and expensive equipment. These obstacles make it difficult to diagnose B. mallei and B. pseudomallei infections in areas where reagents and equipment are difficult to procure. To solve this problem, we developed an easy and ready-to-use dried-format diagnostic tool based on loop-mediated isothermal amplification (LAMP) method. RESULTS: The primer set targeting the internal transcribed spacer (ITS) region detected 10 genomic copies of B. mallei DNA and B. pseudomallei DNA using the conventional liquid LAMP method. This primer set did not detect any other Burkholderia species. Using this novel primer set, a dried-format in-house LAMP method with high sensitivity and specificity was developed. This method was used to test for the presence of B. mallei DNA in swabs collected from the nasal cavity and ulcerated skin of 19 B. mallei-infected horses and five uninfected horses and was compared with the real-time PCR method. These two tests showed 87.5% agreement for the positive samples and 100% agreement for the negative samples. This method detected all tested B. pseudomallei clinical isolates. CONCLUSIONS: We established the first dry LAMP method for the detection of B. mallei and B. pseudomallei. This study provided a simple, rapid, cost-effective, and sensitive diagnostic tool for glanders and melioidosis. - Unification of symbiotic bacteria during larva-to-adult transition in Culicoides circumscriptus (Diptera: Ceratopogonidae).
Ryo Ozuru; Junya Yamagishi; Ayumi Takeuchi; Yusuke Date; Takatoshi Fujii; Chihiro Sugimoto; Chie Nakajima; Yasuhiko Suzuki; Kaoru Aoki; Jun Fujii; Takashi Matsuba
FEMS microbiology letters, 372, 2025年01月10日, ［国際誌］
英語, 研究論文（学術雑誌）, Blood-sucking midges such as Leptoconops and Culicoides are of medical importance due to their role in causing skin irritation and potentially transmitting pathogens. Investigating their bacterial communities, including possible endosymbionts, may help clarify ecological adaptations and interactions with hosts. Leptoconops nipponensis Tokunaga (Lnt) and Culicoides circumscriptus (Cc), blood-sucking midges, cause severe itching and inflammation in humans. Cc was collected from a small sample of an outbreak swarm of Lnt in the peninsula area of Yonago City, Tottori Prefecture, Japan. This study compared the bacterial flora of Lnt and Cc, revealing distinct bacterial diversity shifts in these insect species between life stages. We analyzed the bacterial communities of adult and larval females of Cc and Lnt using MiSeq sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Notably, alpha diversity in Cc adults was significantly reduced to 1.5 (n = 43), indicating that Cc adults were dominated by a single bacterial genus, compared to 14.9 in Cc larvae (n = 19). BLAST (Basic Local Alignment Search Tool) analysis identified this dominant genus in adult Cc as Rickettsia (Candidatus Tisiphisa), which is known for transovarial transmission in arthropod vectors. In contrast, the bacterial diversity of Lnt showed no significant difference between adults (18.1, n = 32) and larvae (n = 15). These findings suggest that the dominance of Rickettsia in Cc (Candidatus Tisiphisa) adults is linked to their emergence, potentially reflecting differences in reproductive biology and ecological adaptations between these two insect species. Further research is needed to elucidate the functional role of Rickettsia in the life cycle and physiology of Cc. - Surveillance of Escherichia coli From Frozen Chicken Meat in Fiji: Resistance Characteristics and Public Health Concerns.
Deepika Darshani Lata; Rana Fahmi Sabala; Akira Fukuda; Chie Nakajima; Yasuhiko Suzuki; Masaru Usui; Muhammad Asif Zahoor
International journal of food science, 2025, 5487064, 5487064, 2025年, ［国際誌］
英語, 研究論文（学術雑誌）, Antimicrobial resistance (AMR) is a growing concern in human and veterinary medicine. Misuse and overuse of antimicrobials in human medicine, veterinary medicine, agriculture, and aquaculture are major drivers of AMR development, with resistant bacteria also being selected in livestock and transmitted through meat. Research on AMR in livestock and animal-derived foods is lacking in Fiji; thus, the associated risks remain unclear. Chicken is widely consumed in Fiji and is predominantly served frozen. This study is aimed at determining the prevalence and resistance profiles of Escherichia coli in frozen chicken meat from Fijian supermarkets. A total of 100 frozen chicken meat samples were purchased from supermarkets and retail outlets in Fiji for this study. E. coli was isolated from 72% of the samples. The E. coli isolates showed relatively high levels of resistance to ampicillin (36%), tetracycline (24%), and streptomycin (17%). Only one cefotaxime-resistant isolate was obtained, which was identified as an extended-spectrum β-lactamase (ESBL)-producing bacterium. This isolate harbored the ESBL-producing gene bla CTX-M-1 and was classified as ST2522. One colistin-resistant isolate was obtained, and its resistance was attributed to a chromosomal mutation in the pmrB gene. The high level of intestinal bacterial contamination in frozen chicken meat suggests that improved hygiene management is necessary throughout the production and distribution chains. Furthermore, because resistance to antimicrobials is important in both human and veterinary medicine (cefotaxime- and colistin-resistant E. coli), careful monitoring of AMR trends in Fiji is essential. These results suggest that AMR surveillance in meat and livestock is necessary to prevent its spread in Fiji. - Evaluating infection prevention and control programs in Zambian hospitals using the WHO infection prevention and control assessment framework tool.
Steward Mudenda; Joseph Yamweka Chizimu; Linus Ndegwa; Maisa Kasanga; Ilunga Mutwale; Aubrey Chichonyi Kalungia; Evelyn Wesangula; Adriano Focus Lubanga; James C L Mwansa; Martha Mwaba; Amos Yared Massele; Nyambe Sinyange; Tapfumanei Mashe; Malambo Mutila; Paul Simujayang'ombe; David Lowrance; Kaunda Yamba; Misheck Shawa; Chie Nakajima; Yasuhiko Suzuki; John Bwalya Muma; Massimo Sartelli; Brian Godman; Roma Chilengi
Frontiers in public health, 13, 1642119, 1642119, 2025年, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Infection Prevention and Control (IPC) is key to preventing healthcare-associated infections (HAIs) and the spread of antimicrobial resistance (AMR). This study evaluated the implementation of IPC in Zambian hospitals. MATERIALS AND METHODS: We conducted a multicentric cross-sectional study in nine hospitals across Zambia using the WHO IPCAF tool. Data were collected from September 1 to 30, 2024 and analyzed using the self-scoring Excel and IBM SPSS version 25.0. RESULTS: Out of the nine hospitals assessed, four were tertiary-level hospitals while the rest were secondary-level hospitals. Overall, the implementation of IPC core components was intermediate (IPCAF Score of 594 out of 800). Four hospitals had IPCAF scores between 401 and 600, indicating an intermediate level of IPC implementation. Five hospitals scored between 601 and 800, indicating an advanced implementation of IPC in these hospitals. Three tertiary hospitals scored between 601 and 800, demonstrating their advanced implementation of IPC core components. CONCLUSION: This study found that the overall implementation of IPC in the surveyed hospitals was intermediate, indicating that further improvements were needed. There is a need to provide peer-learning support and strengthen IPC implementation to respond to new or re-emerging infections and AMR in the country and beyond. - Antimicrobial Resistance Patterns and Serological Diversity of Shigella Species from Patient Isolates at University Teaching Hospital in Lusaka, Zambia
Nundwe, M.; Chizimu, J.Y.; Mwaba, J.; Shawa, M.; Katete, R.S.; Mutengo, M.M.; Nakazwe, R.; Mulunda, N.R.; Sialubanje, C.; Kalumbi, M.M.; Kaunda, Y.; Chanda, R.; Chambaro, H.; Kamboyi, H.K.; Kapalamula, T.; Mudenda, S.; Chabala, F.W.; Hang?ombe, B.M.; Chilengi, R.; Nakajima, C.; Suzuki, Y.
Bacteria, 4, 2, 2025年
研究論文（学術雑誌） - Spreading Ability of Tet(X)-Harboring Plasmid and Effect of Tetracyclines as a Selective Pressure.
Akira Fukuda; Yuta Kozaki; Cemil Kürekci; Yasuhiko Suzuki; Chie Nakajima; Masaru Usui
Microbial drug resistance (Larchmont, N.Y.), 30, 12, 489, 501, 2024年12月, ［国際誌］
英語, 研究論文（学術雑誌）, Tigecycline is a last-resort antimicrobial in humans. Tetracyclines are the most widely used antimicrobials in livestock. Mobile tigecycline resistance genes [tet(X)] are disseminated worldwide, and tetracycline use may have promoted the selection of tet(X) genes. Thus, the selective pressure on tet(X) genes and their plasmids in livestock must be elucidated. We performed a retrospective study to clarify the prevalence of tigecycline-resistant Escherichia coli from pigs in Thailand. Screening for tigecycline resistance was performed on 107 E. coli strains from 25 samples, and tet(X)-carrying plasmids were characterized. tet(X) genes were cloned and expressed in E. coli. Bacterial growth rate in the presence of tetracycline as a result of the presence of tet(X) genes was also evaluated. Thirty-two tet(X4)-harboring tigecycline-resistant E. coli strains were detected in 10/25 samples (40%). The tet(X4) genes were carried on various Inc-type plasmids and flanked by ISCR2. The tet(X)-carrying plasmids were transferred to E. coli and Klebsiella pneumoniae. Acquisition of tet(X) genes and their plasmids improved bacterial growth in the presence of tetracycline. In summary, tetracycline use exerts selective pressure on tet(X) genes and their various backbone plasmids; therefore, a reduced amount of tetracycline use is important to limit the spreading of tet(X) genes. - Vermicomposting reduces the antimicrobial resistance in livestock waste
Masaru Usui; Akira Fukuda; Takashi Azuma; Yoshihiro Kobae; Yuichi Hori; Mitsutaka Kushima; Satoshi Katada; Chie Nakajima; Yasuhiko Suzuki
Journal of Hazardous Materials Advances, 16, 2024年11月
研究論文（学術雑誌） - Antibiotic use and adherence to the WHO AWaRe guidelines across 16 hospitals in Zambia: a point prevalence survey.
Joseph Yamweka Chizimu; Steward Mudenda; Kaunda Yamba; Chileshe Lukwesa; Raphael Chanda; Ruth Nakazwe; Misheck Shawa; Herman Chambaro; Harvey K Kamboyi; Aubrey Chichonyi Kalungia; Duncan Chanda; Sombo Fwoloshi; Elimas Jere; Tiza Mufune; Derick Munkombwe; Peter Lisulo; Tebuho Mateele; Jeewan Thapa; Kenneth Kapolowe; Nyambe Sinyange; Cephas Sialubanje; Nathan Kapata; Mirfin Mpundu; Freddie Masaninga; Khalid Azam; Chie Nakajima; Makomani Siyanga; Nathan Nsubuga Bakyaita; Evelyn Wesangula; Martin Matu; Yasuhiko Suzuki; Roma Chilengi
JAC-antimicrobial resistance, 6, 5, dlae170, 2024年10月, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: The inappropriate use of antibiotics in hospitals contributes to the development and spread of antimicrobial resistance (AMR). This study evaluated the prevalence of antibiotic use and adherence to the World Health Organization (WHO) Access, Watch and Reserve (AWaRe) classification of antibiotics across 16 hospitals in Zambia. METHODS: A descriptive, cross-sectional study employing the WHO Point Prevalence Survey (PPS) methodology and WHO AWaRe classification of antibiotics was conducted among inpatients across 16 hospitals in December 2023, Zambia. Data analysis was performed using STATA version 17.0. RESULTS: Of the 1296 inpatients surveyed in the 16 hospitals, 56% were female, and 54% were aged between 16 and 50 years. The overall prevalence of antibiotic use was 70%. Additionally, 52% of the inpatients received Watch group antibiotics, with ceftriaxone being the most prescribed antibiotic. Slightly below half (48%) of the inpatients received Access group antibiotics. Compliance with the local treatment guidelines was 53%. CONCLUSIONS: This study found a high prevalence of prescribing and use of antibiotics in hospitalized patients across the surveyed hospitals in Zambia. The high use of Watch group antibiotics was above the recommended threshold indicating non-adherence to the WHO AWaRe guidelines for antibiotic use. Hence, there is a need to establish and strengthen antimicrobial stewardship programmes that promote the rational use of antibiotics in hospitals in Zambia. - WQ-3810, a fluoroquinolone with difluoropyridine derivative as the R1 group exerts high potency against quinolone-resistant Campylobacter jejuni.
Kentaro Koide; Hyun Kim; Matthew V X Whelan; Lawrence P Belotindos; Wimonrat Tanomsridachchai; Ruchirada Changkwanyeun; Masaru Usui; Tadhg Ó Cróinín; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Microbiology spectrum, 12, 10, e0432223, 2024年08月20日, ［国際誌］
英語, 研究論文（学術雑誌）, UNLABELLED: Quinolone-resistant Campylobacter jejuni have been increasing worldwide. Quinolones exert their antibacterial activity by inhibiting DNA gyrase, but most of the isolates acquire quinolone resistance via an amino acid substitution in the A subunit of DNA gyrase. WQ-3810 is a quinolone antibiotic that has been reported to have high potency even to DNA gyrase with amino acid substitutions in several bacterial species; however, there was no information on C. jejuni. Hence, this study aimed to evaluate the activity of WQ-3810 to inhibit wild-type/mutant DNA gyrases of C. jejuni and the bacterial growth for accessing the potency for the treatment of quinolone-resistant C. jejuni infection. The inhibitory activity of WQ-3810 was assessed and compared with ciprofloxacin and nalidixic acid by calculating the half maximal inhibitory concentration (IC50) against wild-type/mutant DNA gyrases. Next, the minimum inhibitory concentration (MIC) of WQ-3810 and five other quinolones was determined for C. jejuni including quinolone-resistant strains with amino acid substitutions in GyrA. Furthermore, the interaction between WQ-3810 and wild-type/mutant DNA gyrase was speculated using docking simulations. The IC50 of WQ-3810 against wild-type DNA gyrase was 1.03 µg/mL and not different from that of ciprofloxacin. However, those of WQ-3810 against mutant DNA gyrases were much lower than ciprofloxacin. The MICs of WQ-3810 ranged <0.016-0.031 µg/mL and were the lowest against both quinolone-susceptible and quinolone-resistant strains among the examined quinolones. The results obtained by the docking simulation agreed well with this observation. WQ-3810 seems to be a promising antimicrobial agent for the infections caused by quinolone-resistant C. jejuni. IMPORTANCE: WQ-3810, a relatively new quinolone antibiotic, demonstrates exceptional antibacterial properties against certain pathogens in previous studies. However, its efficacy against quinolone-resistant Campylobacter jejuni was not previously reported. The prevalence of quinolone-resistant C. jejuni as a cause of foodborne illnesses is increasing, prompting this investigation into the effectiveness of WQ-3810 as a countermeasure. This study revealed high inhibitory activity of WQ-3810 against both wild-type and mutant DNA gyrases of C. jejuni. WQ-3810 was equally efficacious as ciprofloxacin against wild-type DNA gyrases but showed superior effectiveness against mutant DNA gyrases. WQ-3810 also demonstrated the lowest minimum inhibitory concentrations, highlighting its enhanced potency against both susceptible and resistant strains of C. jejuni. This observation was well supported by the results of the in silico analysis. Consequently, WQ-3810 exhibits a higher level of bactericidal activity compared to existing quinolones in combating both susceptible and resistant C. jejuni isolates. - Interplay between Amino Acid Substitution in GyrA and QnrB19: Elevating Fluoroquinolone Resistance in Salmonella Typhimurium.
Pondpan Suwanthada; Siriporn Kongsoi; Sasini Jayaweera; Mwangala Lonah Akapelwa; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
ACS infectious diseases, 10, 8, 2785, 2794, 2024年08月09日, ［国際誌］
英語, 研究論文（学術雑誌）, Globally, there have been increasing reports of antimicrobial resistance in nontyphoidal Salmonella (NTS), which can develop into severe and potentially life-threatening diarrhea. This study focuses on the synergistic effects of DNA gyrase mutations and plasmid-mediated quinolone resistance (PMQR) genes, specifically qnrB19, on fluoroquinolone (FQ) resistance in Salmonella Typhimurium. By utilizing recombinant mutants, GyrAS83F and GyrAD87N, and QnrB19's, we discovered a significant increase in fluoroquinolones resistance when QnrB19 is present. Specifically, ciprofloxacin and moxifloxacin's inhibitory concentrations rose 10- and 8-fold, respectively. QnrB19 was found to enhance the resistance capacity of mutant DNA gyrases, leading to high-level FQ resistance. Additionally, we observed that the ratio of QnrB19 to DNA gyrase played a critical role in determining whether QnrB19 could protect DNA gyrase against FQ inhibition. Our findings underscore the critical need to understand these resistance mechanisms, as their coexistence enables bacteria to withstand therapeutic FQ levels, posing a significant challenge to treatment efficacy. - The first insight into Mycobacterium tuberculosis complex isolates in the lower northern region in Thailand.
Janisara Rudeeaneksin; Supranee Bunchoo; Benjawan Phetsuksiri; Sopa Srisungngam; Ratchaneeporn Khummin; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Transactions of the Royal Society of Tropical Medicine and Hygiene, 118, 8, 527, 536, 2024年08月05日, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Tuberculosis (TB) remains an important infectious disease and different genotypes have been reported. This study aimed to investigate the genetic diversity and molecular epidemiology of TB in the lower northern region of Thailand, where genotyping data are limited. METHODS: A total of 159 Mycobacterium tuberculosis complex (MTBC) isolates from this region were genotyped by spoligotyping and the major spoligotypes were further subdivided by the mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) method. RESULTS: Spoligotyping identified 34 types and classified them into 14 clusters. East African-Indian (EAI) groups were the most frequent (44.7%), followed by Beijing (36.5%), with a higher prevalence of drug resistance. By 15-loci MIRU-VNTR typing, the major groups of the Beijing and EAI2_NTB were further differentiated into 44 and 21 subtypes forming 9 and 5 subclusters with cluster rates of 0.26 and 0.44, respectively. The Hunter-Gaston Discriminatory Index among the Beijing and EAI2_NTB groups were 0.987 and 0.931, respectively, indicating high diversity. CONCLUSIONS: This is the first look at the MTBC genotypes in the lower northern region of Thailand, which could aid in understanding the distribution and potential spread of MTBC and Mycobacterium bovis in the target region to support TB control in Thailand. - Hyperthermophilic composting of livestock waste drastically reduces antimicrobial resistance
Usui M; Azuma T; Katada S; Fukuda A; Suzuki Y; Nakajima C; Tamura Y
Waste Management Bulletin, 2, 3, 241, 248, 2024年08月, ［査読有り］, ［招待有り］
英語, 研究論文（学術雑誌） - Epidemiology and molecular characterization of Mycobacterium tuberculosis including a drug-resistant strain associated with mortality of Asian elephants in Nepal 2019-2022.
Arjun Pandit; Jeewan Thapa; Amir Sadaula; Yasuhiko Suzuki; Chie Nakajima; Susan K Mikota; Naresh Subedi; Bijaya Kumar Shrestha; Michito Shimozuru; Bhawana Shrestha; Bijendra Raya; Sanjay Chaudhary; Sarad Paudel; Toshio Tsubota
Tuberculosis (Edinburgh, Scotland), 148, 102550, 102550, 2024年07月26日, ［国際誌］
英語, 研究論文（学術雑誌）, Tuberculosis (TB) is an emerging threat to the survival of elephants in Nepal. We investigated the lung tissue samples from nine elephants that died from 2019 to 2022 in Nepal using culture, conventional PCR, and loop-mediated isothermal amplification (LAMP) and then performed genotyping of five PCR-positive isolates to understand the possible transmission dynamics of Mycobacterium tuberculosis (Mtb). Results showed that two-thirds (6/9) of elephants were confirmed to be infected from Mtb by LAMP, 5/9 by PCR, and 4/9 by culture. Genotyping of Mtb isolates showed that elephants were infected with the Indo-Oceanic and Beijing lineages including an isoniazid-resistant Beijing lineage. MIRU-VNTR-based phylogeny, gyrA, and katG sequencing showed the possibility of ongoing transmission of Indo-Oceanic lineages and likely transmission of the drug-resistant Beijing lineage from human to elephant. Implementation of comprehensive surveillance and preventive measures are urgently needed to address this zoonotic disease and protect elephants from TB in Nepal. - Functional analysis of the Mycobacterium bovis AF2122/97 PhoPR system.
Jose Maria Urtasun-Elizari; Ruoyao Ma; Hayleah Pickford; Damien Farrell; Gabriel Gonzalez; Viktor Perets; Chie Nakajima; Yasuhiko Suzuki; David E MacHugh; Apoorva Bhatt; Stephen V Gordon
Tuberculosis (Edinburgh, Scotland), 148, 102544, 102544, 2024年07月14日, ［国際誌］
英語, 研究論文（学術雑誌）, The PhoPR system is a master regulator in Mycobacterium tuberculosis. A key difference between M. tuberculosis and Mycobacterium bovis is a G71I substitution in the M. bovis PhoR orthologue. Functional studies of the M. bovis PhoPR system have generated conflicting findings, with some research suggesting that the M. bovis PhoPR is defective while others indicate it is functional. We sought to revisit the functionality of the M. bovis PhoPR system. To address this, we constructed a phoPR mutant in the reference strain M. bovis AF2122/97. We employed a combination of growth assays and transcriptomics analyses to assess the phenotype of the mutant vs wild type and complemented strains. We found that the M. bovis AF2122/97 ΔphoPR mutant showed a growth defect on solid and liquid media compared to the wild type and complemented strains. The transcriptome of the M. bovis AF2122/97 ΔphoPR mutant was also altered as compared to wild type, including differential expression of genes involved in lipid metabolism and secretion. Our work provides further insight into the activity of PhoPR in M. bovis and underlines the importance of the PhoPR system as a master regulator of gene expression in the Mycobacterium tuberculosis complex. - Biphasic Medium Using Nicotinamide for Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis.
Waraporn Thuansuwan; Charoen Chuchottaworn; Chie Nakajima; Yasuhiko Suzuki; Nuntaree Chaichanawongsaroj
Antibiotics (Basel, Switzerland), 13, 6, 2024年06月16日, ［国際誌］
英語, 研究論文（学術雑誌）, Reliable drug susceptibility testing of pyrazinamide (PZA) is technically difficult, since PZA activity is pH sensitive. The aim of this study was to evaluate a biphasic medium assay (BMA) for the reliable detection of PZA resistance in Mycobacterium tuberculosis (MTB) using nicotinamide (NIC) as a surrogate for PZA and identifying the appropriate cut-off value for the assay. The PZA susceptibility of 122 multidrug-resistant tuberculosis (MDR-TB) isolates and 39 drug-susceptible tuberculosis (DS-TB) isolates was examined using the BMA with NIC at four different concentrations (250, 500, 1000, and 2000 mg/L) and comparing the results with results from the BACTEC MGIT 960 reference method. Out of 122 MDR-TB isolates, 40 were identified as resistant by the BACTEC MGIT 960 system, of which 92.5% contained mutations within their pncA gene plus promoter region. A minimum inhibitory concentration of NIC ≥ 1000 mg/L was used as the cut-off concentration to define resistance in correlation with the MGIT 960 outcomes. NIC-BMA had a sensitivity of 90.91%, a specificity of 100%, and an accuracy of 97.52% compared with the MGIT 960 method. NIC-BMA is a promising assay to screen PZA resistance in microbiological laboratories without automation or advanced molecular instruments. - Wide distribution of plasmid mediated quinolone resistance gene, qnrS, among Salmonella spp. isolated from canal water in Thailand.
Jirachaya Toyting; Neunghatai Supha; Yuwanda Thongpanich; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki; Fuangfa Utrarachkij
Journal of applied microbiology, 135, 6, 2024年06月03日, ［国際誌］
英語, 研究論文（学術雑誌）, AIMS: This research focused on assessing the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants and antimicrobial susceptibility in Salmonella strains isolated from Thai canal water. METHODS AND RESULTS: From 2016 to 2020, 333 water samples were collected from six canals across Bangkok, Thailand. Salmonella spp. was isolated, PMQR genes were detected through polymerase chain reactions, and the antimicrobial susceptibility was examined using the disk diffusion method. The results indicated a 92.2% prevalence of Salmonella spp. in canal water, being serogroups B and C the most frequently detected. Overall, 35.3% of isolates harbored PMQR genes, being qnrS the most prevalent gene (97.2%, n = 137/141). Other PMQR genes, including qnrB, qnrD, oqxAB, and aac(6')-Ib-cr, were detected. Notably, six isolates harbored multiple PMQR genes. Furthermore, 9.3% and 3.8% of the overall isolates were resistant to nalidixic acid (NAL) and ciprofloxacin (CIP), respectively. PMQR-positive isolates showed higher rates of non-susceptibility to both NAL (48.2%, n = 68/141) and CIP (92.2%, n = 130/141) compared to PMQR-negative isolates (NAL: 8.9%, n = 23/258; CIP: 11.2%, n = 30/258). CONCLUSIONS: The high prevalence of Salmonella spp., significant PMQR-positive, and reduced susceptibility isolates in canal water is of public health concern in Bangkok. - Assessment of antimicrobial resistance laboratory-based surveillance capacity of hospitals in Zambia: findings and implications for system strengthening
K. Yamba; J. Y. Chizimu; S. Mudenda; C. Lukwesa; R. Chanda; R. Nakazwe; B. Simunyola; M. Shawa; A. C. Kalungia; D. Chanda; T. Mateele; J. Thapa; K. Kapolowe; M. L. Mazaba; M. Mpundu; F. Masaninga; K. Azam; C. Nakajima; Y. Suzuki; N. N. Bakyaita; E. Wesangula; M. Matu; R. Chilengi
Journal of Hospital Infection, 148, 129, 137, 2024年06月
研究論文（学術雑誌） - Carbapenem and colistin-resistant hypervirulent Klebsiella pneumoniae: An emerging threat transcending the egyptian food chain.
Rana Fahmi Sabala; Akira Fukuda; Chie Nakajima; Yasuhiko Suzuki; Masaru Usui; Mohamed Elhadidy
Journal of infection and public health, 17, 6, 1037, 1046, 2024年06月, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a great public health problem and is associated with many disease outbreaks and high mortality rates. Alarmingly, K. pneumoniae has been isolated from food in several recent studies. This study aimed to investigate the prevalence and characteristics of CRKP in food samples from Egypt. METHODS: A total of 311 food samples (including 116 minced meat, 92 chicken meat, 75 diced meat, and 28 mutton) were collected from local markets in Egypt and were screened for CRKP with the determination of their antimicrobial resistance profiles. The whole genome sequence was done for 23 CRKP isolates to clarify the relationship between CRKP from food and human cases in Egypt using the SNP core genome. The conjugation probability of the blaNDM-5 harboring plasmid was identified using oriTfinder RESULTS: CRKP was isolated from 11% (35/311) of the samples, with 45.71% (16/35) of them showing resistance to colistin, one of the last-resort options for treating CRKP-mediated infections. In addition to the carbapenem and colistin resistance, the CRKP isolates frequently exhibited resistance to multiple antimicrobials including β-lactams, fluoroquinolones, aminoglycosides, tetracyclines, and chloramphenicol. In addition, most of the CRKP were potentially hypervirulent K. pneumoniae (HvKP) identified as phylogroup Kp1 and of high-risk groups as detected in STs reported in many human outbreaks globally, such as ST383 and ST147. The core-genome phylogeny showed similarities between the isolates from this study and those previously isolated from clinical human samples in Egypt. In addition, analysis of the plasmid on which blaNDM is encoded revealed that several antimicrobial resistance genes such as blaOXA-9, blaCTX-M-15, aac(6')-Ib, qnrS1, and several virulence genes are encoded on the same plasmid. CONCLUSIONS: This study is significant for food safety and public health and is important to further identify the change in the epidemiology of CRKP infections, especially the consumption of contaminated food products. - Association of toxin-producing Clostridioides difficile with piglet diarrhea and potential transmission to humans.
Kouki Takeichi; Akira Fukuda; Chika Shono; Noriyasu Ota; Chie Nakajima; Yasuhiko Suzuki; Masaru Usui
The Journal of veterinary medical science, 86, 7, 769, 776, 2024年05月27日, ［国内誌］
英語, 研究論文（学術雑誌）, The pathogenicity of Clostridioides difficile in piglets remains controversial. It is unknown whether C. difficile control helps protect piglet health. To clarify the association between C. difficile presence and piglet diarrhea, isolates were obtained from piglets with and without diarrhea. In addition, to determine the genetic relationship of C. difficile from pigs and humans, we performed whole-genome sequencing (WGS) of C. difficile isolates. Diarrheal and non-diarrheal stool samples were collected from neonatal piglets from five farms in Japan in 2021. To clarify the relationship between C. difficile derived from pigs and those from human clinical cases, WGS of C. difficile isolates was performed. Toxin-positive C. difficile were significantly more prevalent in piglets with diarrhea, although the overall frequency of C. difficile did not differ between piglets with and without diarrhea. This observation indicates an association between toxin-positive C. difficile and diarrhea in piglets. However, further studies are needed to establish a direct causal relationship and to explore other contributing factors to diarrhea in piglets. WGS results showed that C. difficile sequence type (ST)11 including the hypervirulent PCR ribotype 078 isolates derived from Japanese pigs were closely related to ST11 of overseas strains (human clinical and animal-derived) and a Japanese human clinical strain. Toxin-positive C. difficile may cause diarrhea in piglets and hypervirulent C. difficile are spreading among pigs and human populations worldwide. - Genomic analysis of Salmonella isolated from canal water in Bangkok, Thailand.
Jirachaya Toyting; Narong Nuanmuang; Fuangfa Utrarachkij; Neunghatai Supha; Yuwanda Thongpanich; Pimlapas Leekitcharoenphon; Frank M Aarestrup; Toyotaka Sato; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Microbiology spectrum, 12, 5, e0421623, 2024年05月02日, ［国際誌］
英語, 研究論文（学術雑誌）, Antimicrobial resistance (AMR) poses an escalating global public health threat. Canals are essential in Thailand, including the capital city, Bangkok, as agricultural and daily water sources. However, the characteristic and antimicrobial-resistance properties of the bacteria in the urban canals have never been elucidated. This study employed whole genome sequencing to characterize 30 genomes of a causal pathogenic bacteria, Salmonella enterica, isolated from Bangkok canal water between 2016 and 2020. The dominant serotype was Salmonella Agona. In total, 35 AMR genes and 30 chromosomal-mediated gene mutations were identified, in which 21 strains carried both acquired genes and mutations associated with fluoroquinolone resistance. Virulence factors associated with invasion, adhesion, and survival during infection were detected in all study strains. 75.9% of the study stains were multidrug-resistant and all the strains harbored the necessary virulence factors associated with salmonellosis. One strain carried 20 resistance genes, including mcr-3.1, mutations in GyrA, ParC, and ParE, and typhoid toxin-associated genes. Fifteen plasmid replicon types were detected, with Col(pHAD28) being the most common type. Comparative analysis of nine S. Agona from Bangkok and 167 from public databases revealed that specific clonal lineages of S. Agona might have been circulating between canal water and food sources in Thailand and globally. These findings provide insight into potential pathogens in the aquatic ecosystem and support the inclusion of environmental samples into comprehensive AMR surveillance initiatives as part of a One Health approach. This approach aids in comprehending the rise and dissemination of AMR and devising sustainable intervention strategies.IMPORTANCEBangkok is the capital city of Thailand and home to a large canal network that serves the city in various ways. The presence of pathogenic and antimicrobial-resistant Salmonella is alarming and poses a significant public health risk. The present study is the first characterization of the genomic of Salmonella strains from Bangkok canal water. Twenty-two of 29 strains (75.9%) were multidrug-resistant Salmonella and all the strains carried essential virulence factors for pathogenesis. Various plasmid types were identified in these strains, potentially facilitating the horizontal transfer of AMR genes. Additional investigations indicated a potential circulation of S. Agona between canal water and food sources in Thailand. The current study underscores the role of environmental water in an urban city as a reservoir of pathogens and these data obtained can serve as a basis for public health risk assessment and help shape intervention strategies to combat AMR challenges in Thailand. - Effect of WQ-3334 on Campylobacter jejuni carrying a DNA gyrase with dominant amino acid substitutions conferring quinolone resistance.
Nami Miura-Ajima; Pondpan Suwanthada; Siriporn Kongsoi; Hyun Kim; Ruttana Pachanon; Kentaro Koide; Shigetarou Mori; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 30, 10, 1028, 1034, 2024年04月04日, ［国際誌］
英語, 研究論文（学術雑誌）, INTRODUCTION: Campylobacteriosis stands as one of the most frequent bacterial gastroenteritis worldwide necessitating antibiotic treatment in severe cases and the rise of quinolones-resistant Campylobacter jejuni poses a significant challenge. The predominant mechanism of quinolones-resistance in this bacterium involves point mutations in the gyrA, resulting in amino acid substitution from threonine to isoleucine at 86th position, representing more than 90% of mutant DNA gyrase, and aspartic acid to asparagine at 90th position. WQ-3334, a novel quinolone, has demonstrated strong inhibitory activity against various bacteria. This study aims to investigate the effectiveness of WQ-3334, and its analogues, WQ-4064 and WQ-4065, with a unique modification in R1 against quinolones-resistant C. jejuni. METHODS: The structure-activity relationship of the examined drugs was investigated by measuring IC50 and their antimicrobial activities were accessed by MIC against C. jejuni strains. Additionally, in silico docking simulations were carried out using the crystal structure of the Escherichia coli DNA gyrase. RESULT: WQ-3334 exhibited the lowest IC50 against WT (0.188 ± 0.039 mg/L), T86I (11.0 ± 0.7 mg/L) and D90 N (1.60 ± 0.28 mg/L). Notably, DNA gyrases with T86I substitutions displayed the highest IC50 values among the examined WQ compounds. Moreover, WQ-3334 demonstrated the lowest MICs against wild-type and mutant strains. The docking simulations further confirmed the interactions between WQ-3334 and DNA gyrases. CONCLUSION: WQ-3334 with 6-amino-3,5-difluoropyridine-2-yl at R1 severed as a remarkable candidate for the treatment of foodborne diseases caused by quinolones-resistant C. jejuni as shown by the high inhibitory activity against both wild-type and the predominant quinolones-resistant strains. - Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia.
Chikwanda Chileshe; Misheck Shawa; Nelson Phiri; Joseph Ndebe; Cynthia Sipho Khumalo; Chie Nakajima; Masahiro Kajihara; Hideaki Higashi; Hirofumi Sawa; Yasuhiko Suzuki; Walter Muleya; Bernard Mudenda Hang'ombe
Antibiotics (Basel, Switzerland), 13, 3, 2024年03月15日, ［国際誌］
英語, 研究論文（学術雑誌）, Poultry products in Zambia form an integral part of the human diet in many households, as they are cheap and easy to produce. The burden of poultry diseases has, however, remained a major challenge. Growing consumer demand for poultry products in Zambia has resulted in non-prudent antimicrobial use on farms, intending to prevent and treat poultry diseases for growth optimisation and maximising profits. This cross-sectional study aimed to identify the different types of bacteria causing diseases in chickens in Lusaka and to detect the extended-spectrum lactamase (ESBL)-encoding genes. We collected 215 samples from 91 diseased chickens at three post-mortem facilities and screened them for Gram-negative bacteria. Of these samples, 103 tested positive for various clinically relevant Enterobacteriaceae, including Enterobacter (43/103, 41.7%), Escherichia coli (20/103, 19.4%), Salmonella (10/103, 9.7%), and Shigella (8/103, 7.8%). Other isolated bacteria included Yersinia, Morganella, Proteus, and Klebsiella, which accounted for 21.4%. E. coli, Enterobacter, Salmonella, and Shigella were subjected to antimicrobial susceptibility testing. The results revealed that E. coli, Enterobacter, and Shigella were highly resistant to tetracycline, ampicillin, amoxicillin, and trimethoprim-sulfamethoxazole, while Salmonella showed complete susceptibility to all tested antibiotics. The observed resistance patterns correlated with antimicrobial usage estimated from sales data from a large-scale wholesale and retail company. Six (6/14, 42.9%) E. coli isolates tested positive for blaCTX-M, whilst eight (8/14, 57.1%) Enterobacter samples tested positive for blaTEM. Interestingly, four (4/6, 66.7%) of the E. coli isolates carrying blaCTX-M-positive strains were also positive for blaTEM. Sanger sequencing of the PCR products revealed that five (5/6, 83.3%) of the abovementioned isolates possessed the blaCTX-M-15 allele. The results suggest the presence of potentially pathogenic ESBL-producing Enterobacteriaceae in poultry, threatening public health. - Heated scallop-shell powder and lime nitrogen effectively decrease the abundance of antimicrobial-resistant bacteria in aerobic compost
Masatoshi Enami; Akira Fukuda; Michi Yamada; Yoshihiro Kobae; Chie Nakajima; Yasuhiko Suzuki; Masaru Usui
Environmental Technology & Innovation, 34, 103590, 103590, Elsevier BV, 2024年03月, ［査読有り］
研究論文（学術雑誌） - Transferable linezolid resistance genes (optrA and poxtA) in enterococci derived from livestock compost at Japanese farms.
Akira Fukuda; Chie Nakajima; Yasuhiko Suzuki; Masaru Usui
Journal of global antimicrobial resistance, 2024年02月07日, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVES: Linezolid is a last-resort antimicrobial in human clinical settings to treat multidrug-resistant gram-positive bacterial infections. Mobile linezolid resistance genes (optrA, poxtA, and cfr) have been detected in various sources worldwide. However, the presence of linezolid-not-susceptible bacteria and mobile linezolid resistance genes in Japan remains uncertain. Therefore, we clarified the existence of linezolid-not-susceptible bacteria and mobile linezolid resistance genes in farm environments in Japan. METHODS: Enterococci isolates from feces compost collected from 10 pig and 11 cattle farms in Japan in 2021 were tested for antimicrobial susceptibility and possession of mobile linezolid resistance genes. Whole-genome sequencing of optrA and/or poxtA genes positive-enterococci was performed. RESULTS: Of 103 enterococci isolates, 12 from pig farm compost were not-susceptible (2 resistant and 10 intermediate) to linezolid. These 12 isolates carried mobile linezolid resistance genes on plasmids or chromosomes (5 optrA-positive Enterococcus faecalis, 6 poxtA-positive E. hirae or E. thailandicus, and 1 optrA- and poxtA-positive E. faecium). The genetic structures of optrA- and poxA-carrying plasmids were almost identical to those reported in other countries. These plasmids were capable of transferring among E. faecium and E. faecalis strains. The optrA- and poxtA-positive E. faecium belonged to ST324 (clade A2), a high-risk multidrug-resistant clone. The E. faecalis carrying optrA gene on its chromosome was identified as ST593. CONCLUSIONS: Although linezolid is not used in livestock, linezolid-not-susceptible enterococci could be indirectly selected by frequently used antimicrobials, such as phenicols. Moreover, various enterococci species derived from livestock compost may serve as reservoirs of linezolid resistance genes carried on globally disseminated plasmids and multidrug-resistant high-risk clones. - Correction to: Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 398 Isolated from Pigs and Humans in Thailand (Antibiotics, (2023), 12, 12, (1745), 10.3390/antibiotics12121745)
Narongpun, P.; Chanchaithong, P.; Yamagishi, J.; Thapa, J.; Nakajima, C.; Suzuki, Y.
Antibiotics, 13, 8, 2024年
研究論文（学術雑誌） - Antimicrobial stewardship situation analysis in selected hospitals in Zambia: findings and implications from a national survey.
Joseph Yamweka Chizimu; Steward Mudenda; Kaunda Yamba; Chileshe Lukwesa; Raphael Chanda; Ruth Nakazwe; Bwalya Simunyola; Misheck Shawa; Aubrey Chichonyi Kalungia; Duncan Chanda; Uchizi Chola; Tebuho Mateele; Jeewan Thapa; Kenneth Kapolowe; Mazyanga Lucy Mazaba; Mirfin Mpundu; Freddie Masaninga; Khalid Azam; Chie Nakajima; Yasuhiko Suzuki; Nathan Nsubuga Bakyaita; Evelyn Wesangula; Martin Matu; Roma Chilengi
Frontiers in public health, 12, 1367703, 1367703, 2024年, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Antimicrobial stewardship (AMS) programs are critical in combating antimicrobial resistance (AMR). In Zambia, there is little information regarding the capacity of hospitals to establish and implement AMS programs. The objective of this study was to conduct a baseline assessment of WHO core elements for an AMS program implementation in eight hospitals in Zambia. MATERIALS AND METHODS: We conducted an exploratory cross-sectional study from September 2023 to December 2023 using a self-scoring Periodic National and Healthcare Facility Assessment Tool from the World Health Organization (WHO) policy guidance on integrated AMS activities in human health. Eight public hospitals were surveyed across the five provinces of Zambia. Data was analyzed using the WHO self-scoring tool and thematic analysis. RESULTS: Overall, 62.5% (6/8) of the facilities scored low (below 60%) in implementing AMS programs. Most facilities had challenges with reporting AMS feedback within the hospital (average score = 46%), Drugs and Therapeutics Committee (DTC) functionality (average score = 49%), AMS actions (average score = 50%), education and training (average score = 54%), and leadership commitment to AMS activities (average score = 56%). The overall score for all AMS core elements was average (56%). All the hospitals (100%) did not have an allocated budget for AMS programs. Finally, there were neither antibiograms to guide antimicrobial utilization nor AMS-trained staff in more than 50% of the hospitals surveyed. CONCLUSION: This study found low AMS implementation in these public hospitals, especially where DTCs were non-functional. The identified challenges and gaps require urgent attention for sustainable multidisciplinary AMS programs. - Phylogenetic diversity and virulence gene characteristics of Escherichia coli from pork and patients with urinary tract infections in Thailand.
Pramualchai Ketkhao; Fuangfa Utrarachkij; Nattaya Parikumsil; Kritchai Poonchareon; Anusak Kerdsin; Peeraya Ekchariyawat; Pawarut Narongpun; Chie Nakajima; Yasuhiko Suzuki; Orasa Suthienkul
PloS one, 19, 7, e0307544, 2024年, ［国際誌］
英語, 研究論文（学術雑誌）, Extraintestinal pathogenic Escherichia coli (ExPEC), especially uropathogenic E. coli (UPEC) are responsible for urinary tract infections (UTIs), while diarrheagenic E. coli (DEC) cause foodborne illnesses. These pathogenic E. coli are a serious threat to human health and a public concern worldwide. However, the evidence on pork E. coli (PEC) harboring UPEC virulence-associated genes is currently limited. Therefore, this study aimed to determine the phylogroups, virulence genes, and their association between PEC and UPEC from UTI patients. In this study, 330 E. coli were obtained from archived stock culture isolated from pork (PEC; n = 165) and urine of patients with UTIs (UPEC; n = 165) during 2014-2022. Phylogroups, UPEC- and diarrheagenic E. coli (DEC) associated virulence genes were assessed using PCR assays. The results showed that phylogroups A (50.3%), and B1 (32.1%) were commonly found among PEC whereas phylogroups B2 (41.8%), and C (25.5%) were commonly detected in the UPEC. PEC and UPEC carried similar virulence-associated genes with different percentages. The most frequent UPEC virulence-associated gene among UPEC, and PEC strains was fimH, (93.3%, and 92.1%), followed by iucC (55.2%, and 12.7%), papC (21.8%, and 4.2%), afaC (22.4%, and 0%), hlyCA (17%, and 0.6%), cnf (16.4%, and 0.6%), and sfa/focDE (8.5%, and 4.8%). Additionally, 6 of 27 UPEC virulence-associated gene patterns were found in both PEC and UPEC strains regardless of phylogroups. Furthermore, the DEC virulence-associated genes were found in only 3 strains, one from PEC harboring eae, and two from UPEC carried fimH-bfpA or afaC-CVD432 indicating hybrid strains. Cluster analysis showed a relationship between PEC and UPEC strains and demonstrated that PEC harboring UPEC virulence-associated genes in pork may be associated with UPEC in humans. Food safety and hygiene practices during pork production chain are important procedures for minimizing cross-contamination of these strains that could be transmitted to the consumers. - Application of Genomic Epidemiology of Pathogens to Farmed Yellowtail Fish Mycobacteriosis in Kyushu, Japan
Takayuki Wada; Shiomi Yoshida; Takeshi Yamamoto; Lisa Nonaka; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Masayuki Imajoh
Microbes and Environments, 39, 2, n/a, n/a, Japanese Society of Microbial Ecology, 2024年, ［国内誌］
英語, 研究論文（学術雑誌）, To investigate mycobacterial cases of farmed yellowtail fish in coastal areas of western Japan (Kagoshima, Kyushu), where aquaculture fisheries are active, Mycobacterium pseudoshottsii, the causative agent, was isolated from six neighboring fishing ports in 2012 and 2013. A phylogenetic ana-lysis revealed that the strains isolated from one fishing port were closely related to those isolated from other regions of Japan, suggesting the nationwide spread of a single strain. However, strains from Japan were phylogenetically distinct from those from the Mediterranean and the United States; therefore, worldwide transmission was not observed based on the limited data obtained on the strains exami-ned in this study. The present results demonstrate that a bacterial genomic ana-lysis of infected cases, a mole-cular epidemiology strategy for public health, provides useful data for estimating the prevalence and transmission pathways of M. pseudoshottsii in farmed fish. A bacterial genome ana-lysis of strains, such as that performed herein, may play an important role in monitoring the prevalence of this pathogen in fish farms and possible epidemics in the future as a result of international traffic, logistics, and trade in fisheries. - Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 398 Isolated from Pigs and Humans in Thailand.
Pawarut Narongpun; Pattrarat Chanchaithong; Junya Yamagishi; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Antibiotics (Basel, Switzerland), 12, 12, 2023年12月16日, ［国際誌］
英語, 研究論文（学術雑誌）, Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has been widespread globally in pigs and humans for decades. Nasal colonization of LA-MRSA is regarded as an occupational hazard to people who are regularly involved in livestock production. Our previous study suggested pig-to-human transmission caused by LA-MRSA clonal complex (CC) 398, using traditional molecular typing methods. Instead, this study aimed to investigate the zoonotic transmission of LA-MRSA CC398 using whole genome sequencing (WGS) technologies. A total of 63 LA-MRSA isolates were identified and characterized in Thailand. Further, the 16 representatives of LA-MRSA CC9 and CC398, including porcine and worker isolates, were subjected to WGS on the Illumina Miseq platform. Core-genome single nucleotide polymorphism (SNP)-based analyses verify the zoonotic transmission caused by LA-MRSA CC398 in two farms. WGS-based characterization suggests the emergence of a novel staphylococcal cassette chromosome (SCC) mec type, consisting of multiple cassette chromosome recombinase (ccr) gene complexes via genetic recombination. Additionally, the WGS analyses revealed putative multi-resistant plasmids and several cross-resistance genes, conferring resistance against drugs of last resort used in humans such as quinupristin/dalfopristin and linezolid. Significantly, LA-MRSA isolates, in this study, harbored multiple virulence genes that may become a serious threat to an immunosuppressive population, particularly for persons who are in close contact with LA-MRSA carriers. - Insight into the genetic diversity of Mycobacterium bovis isolated from cattle in Malawi.
Thoko Flav Kapalamula; Joseph Yamweka Chizimu; Mwangala Lonah Akapelwa; David Atomanyi Barnes; Jirachaya Toyting; Precious Bwalya; Linda Basikolo; David Squarre; Herman M Chambaro; Stephen V Gordon; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Research in veterinary science, 164, 105030, 105030, 2023年11月, ［国際誌］
英語, 研究論文（学術雑誌）, We describe the genetic diversity and phylogenetic relationships of Mycobacterium bovis, isolated from cattle in Malawi. Deletion analysis, spoligotyping, and MIRU-VNTR typing were used to genotype the isolates. Combined with a larger dataset from neighboring countries, the overall M. bovis diversity in Southern Africa was contextualized. From the southern and northern regions of Malawi, 24 isolates were confirmed as M. bovis. We pooled data for the central region (60 isolates) from our recent publication to conceptualize the genetic and phylogenetic relationships of M. bovis in Malawi. European 1 was the dominant M. bovis clonal complex, with 10 unique spoligotype patterns, and SB0131 was ubiquitous. High genetic diversity, a low clustering rate, and many singletons, coupled with a low mutation transmission index, infer a low level of recent transmission, and suggest an endemic status of bovine tuberculosis (bTB) in Malawi. M. bovis isolates from Zambia, Mozambique, and South Africa were genetically related to Malawian isolates, whereas Tanzanian isolates were distantly related. The diversity and phylogenetic analysis suggest earlier introductions and maintenance of M. bovis by constant reinfection from reservoir animals. These findings are fundamental to understanding the source and route of infection in order to establish alternative management strategies for bTB. - Exploration of the novel fluoroquinolones with high inhibitory effect against quinolone-resistant DNA gyrase of Salmonella Typhimurium.
Jirachaya Toyting; Nami Miura; Fuangfa Utrarachkij; Wimonrat Tanomsridachchai; Lawrence P Belotindos; Pondpan Suwanthada; Thoko Flav Kapalamula; Siriporn Kongsoi; Kentaro Koide; Hyun Kim; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Microbiology spectrum, 11, 6, e0133023, 2023年10月05日, ［国際誌］
英語, 研究論文（学術雑誌）, Quinolone-resistant nontyphoidal Salmonella, one of the prominent pathogens causing acute gastroenteritis, has become a public health concern globally. The World Health Organization has ranked fluoroquinolone-resistant Salmonella as a high-priority pathogen for researching and developing new antibiotics. WQ-3034 and WQ-3154 are relatively new synthetic fluoroquinolones with distinctive structures. WQ-3034 has 6-amino-3,5-difluoropyridine-2-yl at R1, 3-hydroxyazetidinyl at R7, and the addition of chlorine atom at R8. WQ-3154 has a similar basic pharmacophore to WQ-3034 except for the modification at R8 with a methyl group. In this study, the inhibitory effect and DNA cleavage effect against wild-type (WT) and mutant Salmonella Typhimurium DNA gyrases of WQ-3034 and WQ-3154 were examined along with WQ-3810 and ciprofloxacin by measuring the drug concentration that inhibits half of the enzyme activity (IC50) and the drug concentration that induces 25% of maximum DNA cleavage (CC25). The minimum inhibitory concentration (MIC) of the compounds was assessed against Salmonella Typhimurium and Salmonella Enteritidis. Among four compounds, WQ-3034 demonstrated the highest inhibitory effect against both WT and mutant Salmonella Typhimurium DNA gyrases with amino acid substitution at codon 83 and/or 87, while ciprofloxacin showed the lowest inhibitory effect. Remarkably, WQ-3034 and WQ-3154 exhibited a significantly higher inhibitory effect than ciprofloxacin against Salmonella Typhimurium DNA gyrase with double amino acid substitution, Ser83Phe-Asp87Asn. Similarly, CC25 of WQ-3034 against mutant Salmonella Typhimurium DNA gyrase was lower than ciprofloxacin. Notably, MICs of WQ-3034 and WQ-3154 were higher than ciprofloxacin. In conclusion, this study revealed that WQ-3034 and WQ-3154 could potentially be effective therapeutic agents against quinolone-resistant nontyphoidal Salmonella. IMPORTANCE Quinolone-resistant nontyphoidal Salmonella is a pressing public health concern, demanding the exploration of novel treatments. In this study, we focused on two innovative synthetic fluoroquinolones, WQ-3034 and WQ-3154. Our findings revealed that these new compounds demonstrate potent inhibitory effects, even against mutant strains that cause resistance to existing quinolones. Hence, WQ-3034 and WQ-3154 could potentially be effective therapeutic agents against quinolone-resistant Salmonella Typhimurium. Furthermore, the data obtained in this study will be baseline information for antimicrobial drug development. - The Impact of Substitutions at Positions 1 and 8 of Fluoroquinolones on the Activity Against Mutant DNA Gyrases of Salmonella Typhimurium.
Pondpan Suwanthada; Siriporn Kongsoi; Nami Miura; Lawrence P Belotindos; Chayada Piantham; Jirachaya Toyting; Mwangala L Akapelwa; Ruttana Pachanon; Kentaro Koide; Hyun Kim; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Microbial drug resistance (Larchmont, N.Y.), 29, 12, 552, 560, 2023年10月04日, ［国際誌］
英語, 研究論文（学術雑誌）, Although many drug-resistant nontyphoidal Salmonella (NTS) infections are reported globally, their treatment is challenging owing to the ineffectiveness of the currently available antimicrobial drugs against resistant bacteria. It is therefore essential to discover novel antimicrobial drugs for the management of these infections. In this study, we report high inhibitory activities of the novel fluoroquinolones (FQs; WQ-3810 and WQ-3334) with substitutions at positions R-1 by 6-amino-3,5-difluoropyridine-2-yl and R-8 by methyl group or bromine, respectively, against wild-type and mutant DNA gyrases of Salmonella Typhimurium. The inhibitory activities of these FQs were assessed against seven amino acid substitutions in DNA gyrases conferring FQ resistance to S. Typhimurium, including high-level resistant mutants, Ser83Ile and Ser83Phe-Asp87Asn by in vitro DNA supercoiling assay. Drug concentrations of WQ compounds with 6-amino-3,5-difluoropyridine-2-yl that suppressed DNA supercoiling by 50% (IC50) were found to be ∼150-fold lower than ciprofloxacin against DNA gyrase with double amino acid substitutions. Our findings highlight the importance of the chemical structure of an FQ drug on its antimicrobial activity. Particularly, the presence of 6-amino-3,5-difluoropyridine-2-yl at R-1 and either methyl group or bromine at R-8 of WQ-3810 and WQ-3334, respectively, was associated with improved antimicrobial activity. Therefore, WQ-3810 and WQ-3334 are promising candidates for use in the treatment of patients infected by FQ-resistant Salmonella spp. - Development of a piezo biosensor for pathogen-specific biopolymer detection using a self-assembly barium titanate/polyvinylidene fluoride composite material
Mariko Takeda; Hayato Yoshino; Haruna Yamazaki; Takamichi Hirata; Takashi Kuroiwa; Chie Nakajima; Yasuhiko Suzuki; Fumio Munakata
Sensors and Actuators A: Physical, 360, 114545, 114545, Elsevier BV, 2023年10月
研究論文（学術雑誌） - Retrospective identification of pathogenic mycobacterial species in fish: Mycobacterium pseudoshottsii YM-3, isolated from a yellowtail fish in 1986 in Kochi, Japan
Masayuki Imajoh; Shiomi Yoshida; Lisa Nonaka; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Takayuki Wada
Microbiology Resource Announcements, 12, 10, e0049823, American Society for Microbiology, 2023年09月15日, ［国際誌］
英語, 研究論文（学術雑誌）, ABSTRACT
The complete genome sequence of mycobacterial strain YM-3, isolated from cultured yellowtail in 1986, was determined. The strain was Mycobacterium pseudoshottsii , a closely related subspecies of Mycobacterium marinum , so the strain was isolated earlier than the first report of the subspecies in 2005. - Molecular Characterisation of Mycobacterium bovis Isolates from Cattle Slaughtered in Adamawa and Gombe States, North-Eastern Nigeria
Sadiq Mohammed Damina; David Atomanyi Barnes; Bitrus Inuwa; Gulak Hussaini Ularamu; Mohammed Bello; Olu Solomon Okaiyeto; Ayuba Caleb Kudi; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Current Issues in Molecular Biology, 45, 7, 6055, 6066, MDPI AG, 2023年07月19日, ［国際誌］
英語, 研究論文（学術雑誌）, Bovine tuberculosis is endemic in Nigeria with control measures as provided by the laws of the country being minimally enforced mostly at the abattoirs only. This study focused on bovine tuberculosis in Adamawa and Gombe States. Tuberculosis lesions were observed in 183 of 13,688 slaughtered cattle in the regions between June and December 2020. Analysis of tissue samples resulted in 17 Mycobacterium bovis isolates, predominantly from Gombe State. Spoligotyping identified four spoligotypes, including SB0944, SB1025, SB1104, and one novel pattern. MIRU-VNTR analysis further differentiated these spoligotypes into eight profiles. All isolates belonged to the Af1 clonal complex. The study emphasises the need for broader coverage and more isolates to comprehensively understand the molecular epidemiology of bovine tuberculosis in Nigeria. To enhance research and surveillance, a cost-effective approach is proposed, utilising a discriminatory VNTR panel comprising five or nine loci. The five-locus panel consists of ETR-C, QUB26, QUB11b, MIRU04, and QUB323. Alternatively, the nine-locus panel includes ETR-A, ETR-B, QUB11a, and MIRU26. Implementing this approach would provide valuable insights into the genetic diversity of M. bovis strains in Nigeria. These findings are crucial for developing effective control measures and minimising the impact of bovine tuberculosis on both animal and human health. - High prevalence of colistin heteroresistance in specific species and lineages of Enterobacter cloacae complex derived from human clinical specimens.
Shota Fukuzawa; Toyotaka Sato; Kotaro Aoki; Soh Yamamoto; Noriko Ogasawara; Chie Nakajima; Yasuhiko Suzuki; Motohiro Horiuchi; Satoshi Takahashi; Shin-Ichi Yokota
Annals of clinical microbiology and antimicrobials, 22, 1, 60, 60, 2023年07月15日, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Colistin (CST) is a last-line drug for multidrug-resistant Gram-negative bacterial infections. CST-heteroresistant Enterobacter cloacae complex (ECC) has been isolated. However, integrated analysis of epidemiology and resistance mechanisms based on the complete ECC species identification has not been performed. METHODS: Clinical isolates identified as "E. cloacae complex" by MALDI-TOF MS Biotyper Compass in a university hospital in Japan were analyzed. Minimum inhibitory concentrations of CST were determined by the broth microdilution method. The population analysis profiling (PAP) was performed for detecting the heteroresistant phenotype. The heat shock protein 60 (hsp60) cluster was determined from its partial nucleotide sequence. From the data of whole-genome sequencing, average nucleotide identity (ANI) for determining ECC species, multilocus sequence type, core genome single-nucleotide-polymorphism-based phylogenetic analysis were performed. phoPQ-, eptA-, and arnT-deleted mutants were established to evaluate the mechanism underlying colistin heteroresistance. The arnT mRNA expression levels were determined by reverse transcription quantitative PCR. RESULTS: Thirty-eight CST-resistant isolates, all of which exhibited the heteroresistant phenotype by PAP, were found from 138 ECC clinical isolates (27.5%). The prevalence of CST-resistant isolates did not significantly differ among the origin of specimens (29.0%, 27.8%, and 20.2% for respiratory, urine, and blood specimens, respectively). hsp60 clusters, core genome phylogeny, and ANI revealed that the CST-heteroresistant isolates were found in all or most of Enterobacter roggenkampii (hsp60 cluster IV), Enterobacter kobei (cluster II), Enterobacter chuandaensis (clusters III and IX), and Enterobacter cloacae subspecies (clusters XI and XII). No heteroresistant isolates were found in Enterobacter hormaechei subspecies (clusters VIII, VI, and III) and Enterobacter ludwigii (cluster V). CST-induced mRNA upregulation of arnT, which encodes 4-amino-4-deoxy-L-arabinose transferase, was observed in the CST-heteroresistant isolates, and it is mediated by phoPQ pathway. Isolates possessing mcr-9 and mcr-10 (3.6% and 5.6% of total ECC isolates, respectively) exhibited similar CST susceptibility and PAP compared with mcr-negative isolates. CONCLUSIONS: Significant prevalence (approximately 28%) of CST heteroresistance is observed in ECC clinical isolates, and they are accumulated in specific species and lineages. Heteroresistance is occurred by upregulation of arnT mRNA induced by CST. Acquisition of mcr genes contributes less to CST resistance in ECC. - Genomic Analysis of Mycobacterium tuberculosis Strains Resistant to Second-Line Anti-Tuberculosis Drugs in Lusaka, Zambia
Joseph Yamweka Chizimu; Eddie Samuneti Solo; Precious Bwalya; Thoko Flav Kapalamula; Kaemba Kunkuta Mwale; David Squarre; Misheck Shawa; Patrick Lungu; David Atomanyi Barnes; Kaunda Yamba; Tiza Mufune; Herman Chambaro; Harvey Kamboyi; Musso Munyeme; Bernard Mudenda Hang’ombe; Nathan Kapata; Victor Mukonka; Roma Chilengi; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Antibiotics, 12, 7, 1126, 1126, MDPI AG, 2023年06月29日, ［国際誌］
英語, 研究論文（学術雑誌）, The emergence of pre-extensively drug-resistant tuberculosis (pre-XDR-TB) is a threat to TB control programs in developing countries such as Zambia. Studies in Zambia have applied molecular techniques to understand drug-resistance-associated mutations, circulating lineages and transmission patterns of multi-drug-resistant (MDR) Mycobacterium tuberculosis. However, none has reported genotypes and mutations associated with pre-XDR TB. This study characterized 63 drug-resistant M. tuberculosis strains from the University Teaching Hospital between 2018 and 2019 using targeted gene sequencing and conveniently selected 50 strains for whole genome sequencing. Sixty strains had resistance mutations associated to MDR, one polyresistant, and two rifampicin resistant. Among MDR strains, seven percent (4/60) had mutations associated with pre-XDR-TB. While four, one and nine strains had mutations associated with ethionamide, para-amino-salicylic acid and streptomycin resistances, respectively. All 50 strains belonged to lineage 4 with the predominant sub-lineage 4.3.4.2.1 (38%). Three of four pre-XDR strains belonged to sub-lineage 4.3.4.2.1. Sub-lineage 4.3.4.2.1 strains were less clustered when compared to sub-lineages L4.9.1 and L4.3.4.1 based on single nucleotide polymorphism differences. The finding that resistances to second-line drugs have emerged among MDR-TB is a threat to TB control. Hence, the study recommends a strengthened routine drug susceptibility testing for second-line TB drugs to stop the progression of pre-XDR to XDR-TB and improve patient treatment outcomes. - Colistin-resistant bacteria poses few risks under physiological conditions
Soh Yamamoto; Masaru Usui; Noriko Ogasawara; Wataru Hayashi; Masato Suzuki; Noriyuki Nagano; Chie Nakajima; Yasuhiko Suzuki; Motohiro Horiuchi; Satoshi Takahashi; Shin-ichi Yokota; Yutaka Tamura; Toyotaka Sato
Research Square, Research Square Platform LLC, 2023年06月21日
研究論文（学術雑誌）, Abstract
Globally, 5.0 million people die annually from infections associated with antimicrobial-resistant bacteria, most commonly Escherichia coli1. As colistin is a last-resort antibiotic for multidrug-resistant bacterial infections, the global spread of plasmid-mediated colistin resistance genes (mcr) gene is considered a major public health risk2-4. However, the actual health risks of colistin resistance in hazardous bacteria have never been evaluated under physiological conditions. Here, we show that the fitness/virulence and colistin resistance of the pandemic multidrug-resistant E. coli clone ST1315 very depending on the acquired colistin resistance determinants and differ between physiological and in vitro conditions. The fitness/virulence of ST131 was unaffected by chromosomal-gene (pmrB) mutations or the acquisition of mcr-5-harbouring plasmids in mouse models. However, the acquisition of mcr-1- or mcr-3-harbouring plasmids attenuated fitness/virulence and promoted colistin susceptibility in human serum. We identified two virulence attenuation factors (vafA and vafB) on the pIncI2_mcr-1 plasmid that hijacked the ST131 transcriptome and inhibited nucleotide synthesis, attenuating colistin resistance. Our results demonstrate that colistin resistance poses much less of a threat than believed6,7. We suggest that “nonresistance genes,” rather than resistance genes, are important antimicrobial resistance determinants for human health because they determine fitness/virulence and ultimately antimicrobial susceptibility under physiological conditions. - Characterization of DNA Gyrase Activity and Elucidation of the Impact of Amino Acid Substitution in GyrA on Fluoroquinolone Resistance in Mycobacterium avium.
Jeewan Thapa; Joseph Yamweka Chizimu; Soyoka Kitamura; Mwangala Lonah Akapelwa; Pondpan Suwanthada; Nami Miura; Jirachaya Toyting; Tomoyasu Nishimura; Naoki Hasegawa; Yukiko Nishiuchi; Stephen V Gordon; Chie Nakajima; Yasuhiko Suzuki
Microbiology spectrum, e0508822, 2023年04月17日, ［国際誌］
英語, 研究論文（学術雑誌）, Mycobacterium avium, a member of the M. avium complex (MAC), is the major pathogen contributing to nontuberculous mycobacteria (NTM) infections worldwide. Fluoroquinolones (FQs) are recommended for the treatment of macrolide-resistant MACs. The association of FQ resistance and mutations in the quinolone resistance-determining region (QRDR) of gyrA of M. avium is not yet clearly understood, as many FQ-resistant clinical M. avium isolates do not have such mutations. This study aimed to elucidate the role of amino acid substitution in the QRDR of M. avium GyrA in the development of FQ resistance. We found four clinical M. avium subsp. hominissuis isolates with Asp-to-Gly change at position 95 (Asp95Gly) and Asp95Tyr mutations in gyrA that were highly resistant to FQs and had 2- to 32-fold-higher MICs than the wild-type (WT) isolates. To clarify the contribution of amino acid substitutions to FQ resistance, we produced recombinant WT GyrA, GyrB, and four GyrA mutant proteins (Ala91Val, Asp95Ala, Asp95Gly, and Asp95Tyr) to elucidate their potential role in FQ resistance, using them to perform FQ-inhibited DNA supercoiling assays. While all the mutant GyrAs contributed to the higher (1.3- to 35.6-fold) FQ 50% inhibitory concentration (IC50) than the WT, Asp95Tyr was the most resistant mutant, with an IC50 15- to 35.6-higher than that of the WT, followed by the Asp95Gly mutant, with an IC50 12.5- to 17.6-fold higher than that of the WT, indicating that these amino acid substitutions significantly reduced the inhibitory activity of FQs. Our results showed that amino acid substitutions in the gyrA of M. avium contribute to FQ resistance. IMPORTANCE The emergence of fluoroquinolone (FQ) resistance has further compounded the control of emerging Mycobacterium avium-associated nontuberculous mycobacteria infections worldwide. For M. avium, the association of FQ resistance and mutations in the quinolone resistance-determining region (QRDR) of gyrA is not yet clearly understood. Here, we report that four clinical M. avium isolates with a mutation in the QRDR of gyrA were highly resistant to FQs. We further clarified the impact of mutations in the QRDR of GyrA proteins by performing in vitro FQ-inhibited DNA supercoiling assays. These results confirmed that, like in Mycobacterium tuberculosis, mutations in the QRDR of gyrA also strongly contribute to FQ resistance in M. avium. Since many FQ-resistant M. avium isolates do have these mutations, the detailed molecular mechanism of FQ resistance in M. avium needs further exploration. - Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi.
Thoko Flav Kapalamula; Jeewan Thapa; Kyoko Hayashida; Joseph Chizimu; Wimonrat Tanomsridachchai; Mirriam Ethel Nyenje; Rajab Mkakosya; Chie Nakajima; Yasuhiko Suzuki
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 10406387231164596, 10406387231164596, 2023年04月08日, ［国際誌］
英語, 研究論文（学術雑誌）, The lack of quick, accurate, and low-cost detection methods has hindered the active control strategies for bovine tuberculosis (bTB) in resource-limited countries with a high burden of disease. We developed a dry loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Mycobacterium bovis, the principal causative agent of bTB, and evaluated the efficacy of the assay using suspected bTB samples collected during routine meat inspection at major regional abattoirs in Malawi. Template genomic DNA was extracted directly from the granulomatous bTB-like lesion (crude extracted DNA), as well as growth from the incubated mycobacterial growth indicator tubes (MGIT). Field results were visualized by the naked eye within 40 min following a color change of the amplified products. The sensitivity and specificity of the dry LAMP assay while using 152 DNA samples extracted from MGIT with confirmed M. bovis results were 98% and 88%, respectively. When 43 randomly selected crude DNA samples from lesions were used, the sensitivity and specificity of the dry LAMP assay were 100% and 75%, respectively. Our LAMP assay offers the potential to meet the demands for a low-cost and rapid field detection tool for bTB in resource-limited countries in which bTB is endemic. - Determination of the Prevalence and Antimicrobial Resistance of Enterococcus faecalis and Enterococcus faecium Associated with Poultry in Four Districts in Zambia.
Grace Mwikuma; Henson Kainga; Simegnew Adugna Kallu; Chie Nakajima; Yasuhiko Suzuki; Bernard Mudenda Hang'ombe
Antibiotics (Basel, Switzerland), 12, 4, 2023年03月28日, ［国際誌］
英語, 研究論文（学術雑誌）, The presence of antimicrobial-resistant Enterococci in poultry is a growing public health concern worldwide due to its potential for transmission to humans. The aim of this study was to determine the prevalence and patterns of antimicrobial resistance and to detect drug-resistant genes in Enterococcus faecalis and E. faecium in poultry from four districts in Zambia. Identification of Enterococci was conducted using phenotypic methods. Antimicrobial resistance was determined using the disc diffusion method and antimicrobial resistance genes were detected using polymerase chain reaction and gene-specific primers. The overall prevalence of Enterococci was 31.1% (153/492, 95% CI: 27.1-35.4). Enterococcus faecalis had a significantly higher prevalence at 37.9% (58/153, 95% CI: 30.3-46.1) compared with E. faecium, which had a prevalence of 10.5% (16/153, 95% CI: 6.3-16.7). Most of the E. faecalis and E. faecium isolates were resistant to tetracycline (66/74, 89.2%) and ampicillin and erythromycin (51/74, 68.9%). The majority of isolates were susceptible to vancomycin (72/74, 97.3%). The results show that poultry are a potential source of multidrug-resistant E. faecalis and E. faecium strains, which can be transmitted to humans. Resistance genes in the Enterococcus species can also be transmitted to pathogenic bacteria if they colonize the same poultry, thus threatening the safety of poultry production, leading to significant public health concerns. - Corrigendum to 'Clonal/subclonal changes and accumulation of CTX-M-type β-lactamase genes in fluoroquinolone-resistant Escherichia coli ST131 and ST1193 strains isolated during the past 12 years, Japan' [Journal of Global Antimicrobial Resistance 27 (2021) 150-155].
Yukari Fukushima; Toyotaka Sato; Naoyuki Tsukamoto; Chie Nakajima; Yasuhiko Suzuki; Satoshi Takahashi; Shin-Ichi Yokota
Journal of global antimicrobial resistance, 32, 195, 195, 2023年03月, ［国際誌］
英語 - Prevalence and risk factors of bovine tuberculosis in slaughtered cattle, Malawi.
Thoko Flav Kapalamula; Francis Kawonga; Misheck Shawa; Joseph Chizimu; Jeewan Thapa; Mirriam Ethel Nyenje; Rajhab Sawasawa Mkakosya; Kyoko Hayashida; Stephen Gordon; Chie Nakajima; Musso Munyeme; Bernard M Hang'ombe; Yasuhiko Suzuki
Heliyon, 9, 2, e13647, 2023年02月, ［国際誌］
英語, 研究論文（学術雑誌）, Bovine tuberculosis (bTB) is an infectious disease with significant socioeconomic, animal, and public health impacts. However, the prevalence of bTB remains largely unclear in Malawi due to a paucity of information. Additionally, the existence of multiple risk factors is postulated to enhance bTB transmission in animals. A cross-sectional survey to estimate the prevalence of bTB, animal characteristics and identify associated risk factors was conducted from slaughtered cattle at three major regional abattoirs (southern, central and northern regions) in Malawi. Out of a total of 1547 cattle examined, 154 (9.95%) had bTB-like lesions in various visceral organs and lymph nodes; one sample per animal was collected, processed, and cultured in the in the BACTEC Mycobacterial growth indicator tube (MGIT) 960 system. From the 154 cattle that showed tuberculous like lesions, only 112 were positive on MGIT and 87 were confirmed to have M. bovis based on multiplex PCR. Cattle from the southern region (odds ratio (OR) = 1.96, 95% CI: 1.03-3.85) and central region (OR = 2.00, 95% CI: 1.16-3.56) were more likely presented with bTB-like lesions at slaughter than from the northern region. The risk of having bTB-like lesions was higher in females (OR = 1.51, CI: 1.00-2.29), older cattle (OR = 2.17, CI: 1.34-3.37), and crossbreeds (OR = 1.67, 95% CI: 1.12-2.47) than in males, younger animals, and Malawi Zebu breed, respectively. The high prevalence of bTB is of critical concern and necessitates active surveillance and strengthening of the current control strategies under a One Health (OH) approach at the animal-human interface. - Sensitive detection of Mycobacterium bovis in spiked milk using a polymerase chain reaction assay
Marvin A. Villanueva; Yasuhiko Suzuki; Chie Nakajima; Haruka Suzuki; Yukari Fukushima; Claro N. Mingala
Archives of Veterinary Science, 28, 4, 1, 6, 2023年
研究論文（学術雑誌） - Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies.
Hiroto Takeuchi; Chie Nakajima; Satoru Konnai; Naoya Maekawa; Tomohiro Okagawa; Masaru Usui; Yutaka Tamura; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
PloS one, 18, 1, e0281171, 2023年, ［国際誌］
英語, 研究論文（学術雑誌）, Coagulase-positive Staphylococci express protein A, which binds to host antibodies, to evade the immune system. Taking advantage of its specific binding to antibodies, protein A from Staphylococcus aureus, which is called SpA, is commonly used as an affinity chromatography ligand for human therapeutic antibodies. However, among four canine IgG subclasses (A, B, C, and D), only IgG-B binds to SpA strongly and establishing an efficient and robust purification scheme for canine therapeutic antibodies whose IgG subclass is A, C, or D remains difficult and depends on finding a suitable substitute to SpA. S. pseudintermedius, a major coagulase-positive Staphylococci found in dogs, expresses spsQ gene which is orthologous to S. aureus spa. We hypothesized that to serve S. pseudintermedius to better adapt to the dog immune system, SpsQ would bind to canine IgGs stronger than SpA, making it a better affinity chromatography ligand for canine therapeutic antibodies. To characterize SpsQ, we first determined the spsQ nucleotide sequence from S. pseudintermedius isolates. Based on the identified sequence, we prepared recombinant proteins containing the immunoglobulin-binding domains of SpA (r-SpA) and SpsQ (r-SpsQ) and determined their binding capacity for each canine IgG subclass. The binding capacity of r-SpsQ for IgG-B was almost as high as that of r-SpA. Interestingly, while both r-SpsQ and r-SpA showed no binding to IgG-C, the binding capacity of r-SpsQ for IgG-A and IgG-D was significantly higher than that of r-SpA. Finally, we performed affinity chromatography using r-SpsQ- or r-SpA-immobilized resin and revealed that the recovery rates of IgG-A and IgG-D using r-SpsQ were significantly higher than those using r-SpA. Our findings indicate that SpsQ has a strong potential to be used as an affinity chromatography ligand for canine therapeutic antibodies of subclass A, B, and D. - In vitro antibacterial activity of OPS-2071 against Gram-positive and Gram-negative enteropathogenic bacteria.
Daisuke Oka; Ruchirada Changkwanyeun; Tomoyuki Yamaguchi; Chie Nakajima; Yasuhiko Suzuki; Makoto Matsumoto
The Journal of antimicrobial chemotherapy, 2022年09月14日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Enteric infections are a major public health issue in developing countries. Antimicrobial resistance is also a problem for enteric infection. OPS-2071 is a novel quinolone antibiotic with low oral absorption and potent antibacterial activity against Clostridioides difficile. OBJECTIVES: This study was conducted to confirm the antimicrobial activity of OPS-2071 against major enteropathogenic bacteria and to evaluate the risk of emergence of drug resistance. METHODS: The antibacterial activity was evaluated by the agar dilution method. The inhibitory activity against DNA gyrase and topoisomerase IV was determined by supercoiling assay and decatenation assay, respectively. The mutant prevention concentration and frequency of spontaneous resistance were determined by inoculation on drug-containing agar. RESULTS: Compared with the reference drugs, the antibacterial activity of OPS-2071 was more potent against Gram-positive bacteria and Campylobacter jejuni, including quinolone-resistant strains. Against other Gram-negative bacteria, OPS-2071 was comparable to existing quinolones. The inhibitory activities against DNA gyrase with quinolone-resistant mutations closely correlated with the antibacterial activity. Spontaneous resistance to OPS-2071 was not observed in Staphylococcus aureus and Escherichia coli and was lower than that of existing quinolones and higher than that of azithromycin in C. jejuni. The mutant prevention concentration of OPS-2071 was lower than that of tested compounds in S. aureus and C. jejuni and slightly higher than that of existing quinolones in E. coli. CONCLUSIONS: The broad and potent in vitro antibacterial activity and lower risk of drug resistance suggested that OPS-2071 may be useful for enteric infections caused by major pathogens including quinolone-resistant Campylobacter. - Detection of Mutations in pncA in Mycobacterium tuberculosis Clinical Isolates from Nepal in Association with Pyrazinamide Resistance.
Dipti Shrestha; Bhagwan Maharjan; Jeewan Thapa; Mwangala Lonah Akapelwa; Precious Bwalya; Joseph Yamweka Chizimu; Chie Nakajima; Yasuhiko Suzuki
Current issues in molecular biology, 44, 9, 4132, 4141, 2022年09月08日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Without the proper information on pyrazinamide (PZA) susceptibility of Mycobacterium tuberculosis (MTB), PZA is inappropriately recommended for the treatment of both susceptible and multidrug-resistant tuberculosis (MDR-TB) in Nepal. This study aimed to collect information regarding PZA susceptibility in MTB isolates from Nepal by analyzing pncA and its upstream regulatory region (URR). A total of 211 MTB isolates were included in this study. Sequence analysis of pncA and its URR was performed to assess PZA resistance. First-line drug susceptibility testing, spoligotyping, and sequence analysis of rpoB, katG, the inhA regulatory region, gyrA, gyrB, and rrs were performed to assess their association with pncA mutation. Sequencing results reveal that 125 (59.2%) isolates harbored alterations in pncA and its URR. A total of 57 different mutation types (46 reported and 11 novel) were scattered throughout the whole length of the pncA gene. Eighty-seven isolates (41.2%) harbored mutations in pncA, causing PZA resistance in MTB. There was a more significant association of pncA alterations in MDR/pre-extensively drug-resistant (Pre-XDR) TB than in mono-resistant/pan-susceptible TB (p < 0.005). This first report on the increasing level of PZA resistance in DR-TB in Nepal highlights the importance of PZA susceptibility testing before DR-TB treatment. - Molecular characterization of mutations in isoniazid- and rifampicin- resistant Mycobacterium tuberculosis isolated in Thailand.
Janisara Rudeeaneksin; Benjawan Phetsuksiri; Chie Nakajima; Yukari Fukushima; Worasak Suthachai; Nattakan Tipkrua; Krairerk Suthum; Nasron Jekloh; Supranee Bunchoo; Sopa Srisungngam; Wiphat Klayut; Shigeyuki Hamada; Yasuhiko Suzuki
Japanese journal of infectious diseases, 2022年08月31日, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, Drug-resistant tuberculosis (TB) is a great challenge in TB control. The frequency and mutation characteristics can imply the efficiency of molecular tests for the rapid detection of TB drug resistance. This study examined the existence of mutations in katG and inhA for isoniazid (INH) resistance, and rpoB for rifampicin (RIF) resistance. A total of 178 drug-resistant Mycobacterium tuberculosis (MTB) isolates were analyzed. Mutations in katG encoding and inhA regulatory regions were detected in 136/168 (81.0%) and 29/168 (17.3%), respectively, with the most prominent mutation of Ser315Thr substitution in katG in 126/168 (75.0%), and -15 C > T substitution in the regulatory region of the inhA (26/168; 15.5%). Two distinct katG mutations (Tyr337Cys, 1003InsG) were identified. Of 125 RIF-resistant isolates, 118 (94.4%) carried mutations affecting the 81-bp RIF resistance-determining region (RRDR) with the most commonly affected codons 450, 445, and 435 identified in 74 (59.2%), 26 (20.8%) and 12 (9.6%) isolates, respectively. The genetic mutations were highly associated with phenotypic INH and RIF resistance, and the majority shared similarities with those in previous studies in Thailand and other Asian countries. The data is useful for guiding the use and the improvement of molecular tests for TB-drug resistance. - Threat from Mycobacterium orygis-associated tuberculosis in south Asia.
Jeewan Thapa; Stephen V Gordon; Chie Nakajima; Yasuhiko Suzuki
The Lancet. Microbe, 3, 9, e641-e642, 2022年06月14日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - ANTIBIOGRAMS AND VIRULENCE GENES OF ESCHERICHIA COLI AND SALMONELLA SP FROM FARM PIGS IN CENTRAL THAILAND
Pornthip Chompook; Apiradee Intarapak; Kanjana Changkaew; Ruttana Pachanon; Apichart Suwannachairob; Chie Nakajima; Yasuhiko Suzuki; Orasa Suthienkul; Norikazu Isoda
Southeast Asian Journal of Tropical Medicine and Public Health, 53, 3, 322, 344, 2022年05月19日
研究論文（学術雑誌） - Characterisation of plasmids harbouring qnrA1, qnrS1, and qnrB4 in E. coli isolated in the Philippines from food-producing animals and their products.
Lawrence P Belotindos; Risa Tsunoda; Marvin A Villanueva; Chie Nakajima; Claro N Mingala; Yasuhiko Suzuki
Journal of global antimicrobial resistance, 30, 38, 46, 2022年04月18日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVES: Determinants showing plasmid-mediated quinolone resistance, which usually leads to antimicrobial ineffectiveness, have become an emerging clinical problem. In our previous study in the Philippines, a high prevalence of Qnr determinants was found in clinical samples and food-producing animals and their food products. However, no qnr-carrying plasmids have been investigated in animals or animal-derived foods. Hence, in the present, we aimed to characterise qnr-carrying plasmids in Escherichia coli isolated from the food supply chain. METHODS: Plasmids from 44 qnr-positive isolates were assigned to incompatibility groups by PCR-based replicon typing, and the presence of β-lactamase-encoding genes were investigated by PCR. Localisation of qnr in plasmids was determined by S1-PFGE and Southern blot hybridisation. The transferability of qnr-carrying plasmids was examined by conjugation analysis. RESULTS: Overall, 77.3% (95%CI = 62.2 - 88.5) of the isolates harbouring qnr determinants were positive for seven plasmid types, and 56.8% concurrently harboured blaTEM-1. Plasmid IncFrepB was prevalent (65.9%, 95%CI = 50.1 - 79.5) among qnr determinants. Localisation of qnr determinants in IncFrepB and transferability of plasmids was further confirmed. CONCLUSIONS: The current study proved that qnr in E. coli isolated from food-producing animals and their food products could spread via plasmid IncFrepB upon selective pressure with quinolones or other antimicrobials. Therefore, to curb the emergence and spread of qnr-harbouring bacteria in the Philippines, prudent use of antimicrobials in animal production and stricter hygiene and food handling are recommended. - Disseminated tuberculosis with paradoxical reactions caused by a Mycobacterium tuberculosis strain belonging to the Indo-Oceanic lineage: An imported case in Japan.
Kengo Oshima; Chie Nakajima; Kazushige Hirata; Hironori Hayashi; Eiichi N Kodama; Yukari Fukushima; Yasuhiko Suzuki; Hajime Kanamori; Hiroaki Baba; Tetsuji Aoyagi; Koichi Tokuda; Mitsuo Kaku
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 28, 7, 965, 970, 2022年03月04日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Tuberculosis remains a major public health concern. Millions of tuberculosis cases and associated deaths have been reported worldwide. The Indo-Oceanic lineage Mycobacterium tuberculosis is common in Southeast Asia and causes extrapulmonary lesions. Only a few case studies on this lineage with genetic analysis using whole-genome sequencing have been reported in the literature. We present a case of disseminated tuberculosis, characterized by a variety of extrapulmonary lesions and paradoxical reactions, caused by the Indo-Oceanic lineage M. tuberculosis in a woman in Myanmar. A 22-year-old Burmese woman had arthritis in the right knee, with unknown aetiology, and was referred to our hospital. Computed tomography of the trunk revealed multiple nodular shadows in both lungs; swollen mediastinal lymph nodes; and small, low-density areas in the spleen. M. tuberculosis was detected in the sputum sample, joint aspirate, subcutaneous tumor, and exudate. She experienced a variety of paradoxical reactions together with aggressive tuberculosis dissemination in all areas of the body. Whole-genome sequencing of the DNA of MTB obtained from sputum and the right cervical subcutaneous abscess confirmed the Indo-Oceanic lineage of M. tuberculosis, the predominant strain in Myanmar. The Indo-Oceanic lineage M. tuberculosis causes disseminated tuberculosis all over the body including the periungual region. When patients show unusual symptoms, physicians should consider the introduction of new strains from foreign countries. Genetic analyses of the strains are recommended to define and confirm the lineages. - Characterization of embB mutations involved in ethambutol resistance in multi-drug resistant Mycobacterium tuberculosis isolates in Zambia.
Precious Bwalya; Eddie S Solo; Joseph Y Chizimu; Dipti Shrestha; Grace Mbulo; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Tuberculosis (Edinburgh, Scotland), 133, 102184, 102184, 2022年03月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - (Part 1) DNA gyrase could be a crucial regulatory factor for growth and survival of Mycobacterium leprae
Kim, H.; Fukutomi, Y.; Nakajima, C.; Kim, Y.; Mori, S.; Shibayama, K.; Nakata, N.; Suzuki, Y.
Japanese Journal of Leprosy, 91, 2, 2022年
研究論文（学術雑誌） - Conjugative IncHI2/HI2A plasmids harbouring mcr-9 in colistin-susceptible Escherichia coli isolated from diseased pigs in Japan.
Akira Fukuda; Hitomi Nakano; Yasuhiko Suzuki; Chie Nakajima; Masaru Usui
Access microbiology, 4, 11, acmi000454, 2022年, ［国際誌］
英語, 研究論文（学術雑誌）, Colistin is a last resort antimicrobial used for the treatment of gram-negative bacterial infections. Plasmid-mediated colistin resistance (mcr) genes are a cause of global concern, and, thus far, mcr-1-10 have been identified. In a previous study, we screened mcr-1-5 in Escherichia coli derived from diseased pigs in Japan and reported a high prevalence of mcr-1, -3 and -5. However, the previous report on the prevalence of mcr genes was inaccurate. In the present study, we aimed to clarify the prevalence of all reported variants of mcr in E. coli derived from the diseased pigs, which were previously screened for mcr-1-5. Additionally, we also characterized the mcr-9-positive E. coli , which was detected in this study. We screened mcr in 120 E. coli strains from diseased pigs and mcr-positive E. coli and an mcr-carrying plasmid were also characterized. One mcr-9-positive colistin-susceptible E. coli strain was detected (0.8 %). Plasmid-mediated mcr-9 was transferred to E. coli ML4909 as the recipient strain, and it was located on IncHI2/HI2A plasmid p387_L with other antimicrobial resistance genes (ARGs). The region harbouring ARGs including mcr-9, was similar to that on the Klebsiella pneumoniae chromosome harbouring mcr-9 isolated in Japan. mcr-3, -5 and -9 were detected (4.2 %) in colistin-susceptible strains. mcr-9 was found to be disseminated via the plasmid IncHI2/HI2A p387_L and transferred and inserted into chromosomes via a transposon. Our results suggest that mcr genes should be monitored regularly, regardless of their susceptibility to colistin. - Corrigendum: The TbD1 Locus Mediates a Hypoxia-Induced Copper Response in Mycobacterium bovis.
Ruoyao Ma; Damien Farrell; Gabriel Gonzalez; John A Browne; Chie Nakajima; Yasuhiko Suzuki; Stephen V Gordon
Frontiers in microbiology, 13, 947450, 947450, 2022年, ［査読有り］, ［国際誌］
英語, [This corrects the article DOI: 10.3389/fmicb.2022.817952.]. - The TbD1 Locus Mediates a Hypoxia-Induced Copper Response in Mycobacterium bovis.
Ruoyao Ma; Damien Farrell; Gabriel Gonzalez; John A Browne; Chie Nakajima; Yasuhiko Suzuki; Stephen V Gordon
Frontiers in microbiology, 13, 817952, 817952, 2022年, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, The Mycobacterium tuberculosis complex (MTBC) contains the causative agents of tuberculosis (TB) in mammals. The archetypal members of the MTBC, Mycobacterium tuberculosis and Mycobacterium bovis, cause human tuberculosis and bovine tuberculosis, respectively. Although M. tuberculosis and M. bovis share over 99.9% genome identity, they show distinct host adaptation for humans and animals; hence, while the molecular basis of host adaptation is encoded in their genomes, the mechanistic basis of host tropism is still unclear. Exploration of the in vitro phenotypic consequences of known genetic difference between M. bovis and M. tuberculosis offers one route to explore genotype-phenotype links that may play a role in host adaptation. The TbD1 ("Mycobacterium tuberculosis deletion 1 region") locus encompasses the mmpS6 and mmpL6 genes. TbD1 is absent in M. tuberculosis "modern" lineages (Lineages 2, 3, and 4) but present in "ancestral" M. tuberculosis (Lineages 1 and 7), Mycobacterium africanum lineages (Lineages 5 and 6), newly identified M. tuberculosis lineages (Lineages 8 and 9), and animal adapted strains, such as M. bovis. The function of TbD1 has previously been investigated in M. tuberculosis, where conflicting data has emerged on the role of TbD1 in sensitivity to oxidative stress, while the underlying mechanistic basis of such a phenotype is unclear. In this study, we aimed to shed further light on the role of the TbD1 locus by exploring its function in M. bovis. Toward this, we constructed an M. bovis TbD1 knockout (ΔTbD1) strain and conducted comparative transcriptomics to define global gene expression profiles of M. bovis wild-type (WT) and the ΔTbD1 strains under in vitro culture conditions (rolling and standing cultures). This analysis revealed differential induction of a hypoxia-driven copper response in WT and ΔTbD1 strains. In vitro phenotypic assays demonstrated that the deletion of TbD1 sensitized M. bovis to H2O2 and hypoxia-specific copper toxicity. Our study provides new information on the function of the TbD1 locus in M. bovis and its role in stress responses in the MTBC. - Whole-Genome Sequencing Reveals Recent Transmission of Multidrug-Resistant Mycobacterium tuberculosis CAS1-Kili Strains in Lusaka, Zambia.
Joseph Yamweka Chizimu; Eddie Samuneti Solo; Precious Bwalya; Wimonrat Tanomsridachchai; Herman Chambaro; Misheck Shawa; Thoko Flav Kapalamula; Patrick Lungu; Yukari Fukushima; Victor Mukonka; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
Antibiotics (Basel, Switzerland), 11, 1, 2021年12月28日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Globally, tuberculosis (TB) is a major cause of death due to antimicrobial resistance. Mycobacterium tuberculosis CAS1-Kili strains that belong to lineage 3 (Central Asian Strain, CAS) were previously implicated in the spread of multidrug-resistant (MDR)-TB in Lusaka, Zambia. Thus, we investigated recent transmission of those strains by whole-genome sequencing (WGS) with Illumina MiSeq platform. Twelve MDR CAS1-Kili isolates clustered by traditional methods (MIRU-VNTR and spoligotyping) were used. A total of 92% (11/12) of isolates belonged to a cluster (≤12 SNPs) while 50% (6/12) were involved in recent transmission events, as they differed by ≤5 SNPs. All the isolates had KatG Ser315Thr (isoniazid resistance), EmbB Met306 substitutions (ethambutol resistance) and several kinds of rpoB mutations (rifampicin resistance). WGS also revealed compensatory mutations including a novel deletion in embA regulatory region (-35A > del). Several strains shared the same combinations of drug-resistance-associated mutations indicating transmission of MDR strains. Zambian strains belonged to the same clade as Tanzanian, Malawian and European strains, although most of those were pan-drug-susceptible. Hence, complimentary use of WGS to traditional epidemiological methods provides an in-depth insight on transmission and drug resistance patterns which can guide targeted control measures to stop the spread of MDR-TB. - Broad-host-range IncW plasmid harbouring tet(X) in Escherichia coli isolated from pigs in Japan.
Masaru Usui; Akira Fukuda; Yasuhiko Suzuki; Chie Nakajima; Yutaka Tamura
Journal of global antimicrobial resistance, 28, 97, 101, 2021年12月20日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVES: Tetracyclines are used in veterinary medicine for livestock. Tigecycline, a semisynthetic tetracycline derivative, is a last-resort antimicrobial used to treat multidrug-resistant Gram-negative bacterial infections. The prevalence of variants of the mobile tigecycline resistance gene tet(X) in livestock is increasing worldwide. However, the prevalence of Tet(X) among livestock in Japan is unclear. This study was conducted to clarify the prevalence of Tet(X) in pigs in Japan, focusing on isolation and molecular characterisation of plasmid-mediated tet(X)-positive Escherichia coli through retrospective analysis. METHODS: We retrospectively screened for tigecycline-resistant E. coli strains isolated from pigs. The tigecycline-resistant strain and tet(X)-harbouring plasmid were characterised. RESULTS: The IncW plasmid harbouring the tet(X) variant [previously named as tet(X6)] was detected in one E. coli isolate from pigs (0.8%; 1/120) in 2012. The tet(X) plasmid was transferable by conjugation to the E. coli ML4909 recipient strain. Some mobile genetic elements (TnAs3 and ISVsa3) were observed in the region surrounding tet(X). The tet(X)-harbouring plasmid shared a conserved backbone with IncW plasmid R388, which is a broad-host-range plasmid. CONCLUSION: The emergence and spread of tet(X) variants in Enterobacterales poses a public-health concern. To the best of our knowledge, this is the first report of the emergence of an IncW plasmid harbouring tet(X). Using tetracyclines in livestock exerts selective pressure on the tet(X) plasmid; therefore, prudent use of tetracyclines is required. - Clonal/subclonal changes and accumulation of CTX-M-type β-lactamase genes in fluoroquinolone-resistant Escherichia coli ST131 and ST1193 strains isolated during the past 12 years, Japan
Yukari Fukushima; Toyotaka Sato; Naoyuki Tsukamoto; Chie Nakajima; Yasuhiko Suzuki; Satoshi Takahashi; Shin ichi Yokota
Journal of Global Antimicrobial Resistance, 27, 150, 155, 2021年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
Aleksandr Ilinov; Akihito Nishiyama; Hiroki Namba; Yukari Fukushima; Hayato Takihara; Chie Nakajima; Anna Savitskaya; Gebremichal Gebretsadik; Mariko Hakamata; Yuriko Ozeki; Yoshitaka Tateishi; Shujiro Okuda; Yasuhiko Suzuki; Yuri S. Vinnik; Sohkichi Matsumoto
Scientific Reports, 11, 1, 10953, 10953, 2021年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - Evaluation of IS1245 LAMP in Mycobacterium avium and the influence of host-related genetic diversity on its application.
Mwangala Lonah Akapelwa; Thoko Flav Kapalamula; Yuki Ouchi-Aizu; Bernard Mudenda Hang'ombe; Yukiko Nishiuchi; Stephen V Gordon; Eddie Samuneti Solo; Aki Tamaru; Tomoyasu Nishimura; Naoki Hasegawa; Kozo Morimoto; Yukari Fukushima; Yasuhiko Suzuki; Chie Nakajima
Diagnostic microbiology and infectious disease, 101, 4, 115494, 115494, 2021年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan. The developed assay could detect as low as 1 genome copy of M. avium DNA within 30 minutes. All 91 (100%) M. avium isolates from pigs were detected positive while all other tested bacterial species were negative. Interestingly, among the 137 clinical M. avium isolates, 41 (30%) were undetectable with this LAMP assay as they lacked IS1245, the absence of which was revealed by PCR and whole-genome sequencing. These findings highlighted genotypic differences in M. avium strains from humans and pigs in Japan and how this diversity can influence the applicability of a detection tool across different geographic areas and hosts. - Prevalence and Multidrug Resistance of Salmonella in Swine Production Chain in a Central Province, Thailand.
Kaknokrat Chonsin; Ruchirada Changkwanyeun; Achiraya Siriphap; Apiradee Intarapuk; Watsawan Prapasawat; Kanjana Changkaew; Chaiwat Pulsrikarn; Norikazu Isoda; Chie Nakajima; Yasuhiko Suzuki; Orasa Suthienkul
Journal of food protection, 84, 12, 2174, 2184, 2021年12月01日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Salmonella causes foodborne disease outbreaks worldwide and raises considerable concerns about public health and economic losses. To determine prevalence, serovar, antimicrobial resistance (AMR) patterns, and extended-spectrum beta-lactamase (ESBL) genes, the present cross-sectional study collected a total of 418 fecal, carcass (three slaughterhouses), pork and cutting board (four markets) samples from a province in central Thailand in 2017 and 2018. Results showed that 65.1% (272/418) of samples were positive for Salmonella. The percentage of Salmonella positive samples from markets (88.8%; 158/178) was significantly higher than those from slaughterhouses (47.5%; 114/240) ( P<0.05 ). In total, 1,030 isolates were identified; of these, 409 were assigned to 45 serovars with S. Rissen (20%; 82/409) being the most common. New serovars of Thai isolates, S. Cannstatt and S. Braubach, were identified in market and slaughterhouse samples, respectively. AMR of Salmonella isolates showed that 73.9% (133/180) of 19 different serovars exhibited multidrug resistance (MDR). Screening for ESBL production showed that 10.3% (41/399) of isolates were ESBL positive. ESBL-producing Salmonella isolates in market samples (75.6%; 31/41) were significantly higher than those in slaughterhouse samples (24.4%; 10/41) ( P<0.05 ). In market samples, 77.4% (24/31) were isolated from pork and 22.6% (7/31) from cutting boards. Nine ESBL-producing isolates carried single type ESBL genes bla TEM (9.8%; 4/41) or bla CTX-M (12.2%; 5/41), while 11 (26.8%) carried both bla TEM and bla CTX-M . No ESBL-producing Salmonella isolate carried the gene bla SHV . Results suggest that pigs, their flesh, and cutting boards could be reservoirs for widespread MDR, ESBL-producing Salmonella outbreak across the food chain. - Comparative genomic analysis of Mycobacterium intracellulare: implications for clinical taxonomic classification in pulmonary Mycobacterium avium-intracellulare complex disease
Yoshitaka Tateishi; Yuriko Ozeki; Akihito Nishiyama; Mari Miki; Ryoji Maekura; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Sohkichi Matsumoto
BMC Microbiology, 21, 1, 103, 103, 2021年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - PD-L1 immunohistochemistry for canine cancers and clinical benefit of anti-PD-L1 antibody in dogs with pulmonary metastatic oral malignant melanoma
Naoya Maekawa; Satoru Konnai; Maki Nishimura; Yumiko Kagawa; Satoshi Takagi; Kenji Hosoya; Hiroshi Ohta; Sangho Kim; Tomohiro Okagawa; Yusuke Izumi; Tatsuya Deguchi; Yukinari Kato; Satoshi Yamamoto; Keiichi Yamamoto; Mikihiro Toda; Chie Nakajima; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
npj Precision Oncology, 5, 1, 10, 10, 2021年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - Campylobacter Express Resistance Array for detecting the presence of fluoroquinolone- and macrolide-resistant Campylobacter jejuni and Campylobacter coli in broiler farms.
Masaru Usui; Junya Tase; Masanobu Onozaki; Yasuhiko Suzuki; Yutaka Tamura; Chie Nakajima
Journal of applied microbiology, 132, 4, 3249, 3255, 2021年11月30日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - Genetic Diversity and Transmission of Multidrug Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia.
Joseph Yamweka Chizimu; Eddie Samuneti Solo; Precious Bwalya; Thoko Flav Kapalamula; Mwangala Lonah Akapelwa; Patrick Lungu; Dipti Shrestha; Yukari Fukushima; Victor Mukonka; Jeewan Thapa; Chie Nakajima; Yasuhiko Suzuki
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 114, 142, 150, 2021年10月27日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVE: Zambia is among the 30 high tuberculosis burden countries in the world. Despite increasing reports of multidrug resistant tuberculosis (MDR-TB) in routine surveillance, information on the transmission of MDR Mycobacterium tuberculosis strains is largely unknown. This study elucidated genetic diversity and transmission of MDR M. tuberculosis strains in Lusaka, Zambia. METHODS: Eighty-five MDR M. tuberculosis samples collected from the year 2013 to 2017 at the University Teaching Hospital were used. Drug-resistance associated gene sequencing, spoligotyping, 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats, and multiplex PCR for RD-Rio sub-lineage identification were applied. RESULTS: Clades identified were LAM (48%), CAS (29%), T (14%), X (6%) and Harlem (2%). Strains belonging to SITs 21/CAS1-Kili and 20/LAM1 formed the largest clonal complexes. Combined spoligotyping and 24 loci-MIRU-VNTR revealed 47 genotypic patterns with clustering rate of 63%. Ninety five percent of LAM strains belonged to RD-Rio sub-lineage. CONCLUSION: The high clustering rate suggested that a large proportion of MDR-TB was due to recent transmission rather than independent acquisition of MDR. This spread was attributed to clonal expansion of SIT21/CAS1-Kili and SIT20/LAM1 strains. Therefore, TB control programs recommending genotyping coupled with conventional epidemiological methods can guide measures for stopping the spread of MDR-TB. - Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia.
Precious Bwalya; Tomoyuki Yamaguchi; Eddie Samuneti Solo; Joseph Yamweka Chizimu; Grace Mbulo; Chie Nakajima; Yasuhiko Suzuki
Antibiotics (Basel, Switzerland), 10, 10, 2021年09月26日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Streptomycin (STR) is recommended for the management of multidrug-resistant tuberculosis (MDR-TB). Streptomycin resistance-conferring mutation types and frequency are shown to be influenced by genotypes of circulating strains in a population. This study aimed to characterize the mutations in MDR-TB isolates and examine their relationship with the genotypes in Zambia. A total of 138 MDR-TB isolates stored at the University Teaching Hospital Tuberculosis Reference Laboratory in Zambia were analyzed using spoligotyping and sequencing of STR resistance-associated genes. Streptomycin resistance was observed in 65.9% (91/138) of MDR-TB isolates. Mutations in rpsL, rrs, and gidB accounted for 33%, 12.1%, and 49.5%, respectively. Amino acid substitution K43R in rpsL was strongly associated with the CAS1_Kili genotype (p < 0.0001). The combination of three genes could predict 91.2% of STR resistance. Clustering of isolates based on resistance-conferring mutations and spoligotyping was observed. The clustering of isolates suggests that the increase in STR-resistant MDR-TB in Zambia is largely due to the spread of resistant strains from inadequate treatment. Therefore, rapid detection of STR resistance genetically is recommended before its use in MDR-TB treatment in Zambia. - Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG.
Yuki Arimura; Yusuke Minato; Takayuki Wada; Masaaki Nakayama; Ayako Ryumon; Nao Hirata; Chie Nakajima; Yasuhiko Suzuki; Manabu Ato; Kazuo Kobayashi; Naoko Ohara; Seiji Iida; Naoya Ohara
Microbiology and immunology, 66, 1, 10, 14, 2021年09月21日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. In this study, we assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. We successfully constructed a thyX deletion mutant strain from that strain, which reinforces the non-essentiality of thyX under a certain genetic background. This article is protected by copyright. All rights reserved. - Genome Sequences of Two Mycobacterium tuberculosis Isolates from Asian Elephants in Nepal.
Sarad Paudel; Evan P Brenner; Syeda A Hadi; Yasuhiko Suzuki; Chie Nakajima; Toshio Tsubota; Kamal Prasad Gairhe; Bhagwan Maharjan; Srinand Sreevatsan
Microbiology resource announcements, 10, 36, e0061421, 2021年09月09日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, This report describes the genome sequences of two Mycobacterium tuberculosis isolates, S1 and S3, recovered from Asian elephants in Nepal. These genome sequences will enhance our understanding of the genomic epidemiology of Mycobacterium tuberculosis in Asian elephants. - Insights into the ancestry evolution of the Mycobacterium tuberculosis complex from analysis of Mycobacterium riyadhense.
Qingtian Guan; Musa Garbati; Sara Mfarrej; Talal AlMutairi; Thomas Laval; Albel Singh; Shamsudeen Fagbo; Alicia Smyth; John A Browne; Muhammad Amin urRahman; Alya Alruwaili; Anwar Hoosen; Conor J Meehan; Chie Nakajima; Yasuhiko Suzuki; Caroline Demangel; Apoorva Bhatt; Stephen V Gordon; Faisal AlAsmari; Arnab Pain
NAR genomics and bioinformatics, 3, 3, lqab070, 2021年09月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Current evolutionary scenarios posit the emergence of Mycobacterium tuberculosis from an environmental saprophyte through a cumulative process of genome adaptation. Mycobacterium riyadhense, a related bacillus, is being increasingly isolated from human clinical cases with tuberculosis-like symptoms in various parts of the world. To elucidate the evolutionary relationship between M. riyadhense and other mycobacterial species, including members of the M. tuberculosis complex (MTBC), eight clinical isolates of M. riyadhense were sequenced and analyzed. We show, among other features, that M. riyadhense shares a large number of conserved orthologs with M. tuberculosis and shows the expansion of toxin/antitoxin pairs, PE/PPE family proteins compared with other non-tuberculous mycobacteria. We observed M. riyadhense lacks wecE gene which may result in the absence of lipooligosaccharides (LOS) IV. Comparative transcriptomic analysis of infected macrophages reveals genes encoding inducers of Type I IFN responses, such as cytosolic DNA sensors, were relatively less expressed by macrophages infected with M. riyadhense or M. kansasii, compared to BCG or M. tuberculosis. Overall, our work sheds new light on the evolution of M. riyadhense, its relationship to the MTBC, and its potential as a system for the study of mycobacterial virulence and pathogenesis. - Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal.
Jeewan Thapa; Susan K Mikota; Kamal Prasad Gairhe; Sarad Paudel; Dinesh Kumar Singh; Ishwari Prasad Dhakal; Chie Nakajima; Yasuhiko Suzuki
The Journal of veterinary medical science, 83, 8, 1278, 1283, 2021年08月12日, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, We conducted a tuberculosis (TB) serosurveillance program of captive elephants in Nepal and compared hematology and biochemistry parameters between seropositive and seronegative elephants. A total of 153 elephants (male=20, female=133) from four national parks were tested for TB using the ElephantTB STAT-PAK® Assay (ChemBio Diagnostic Systems, Inc., Medford, NY, USA). The mean reported age for 138 elephants was 38.5 years (range 2-71 years). Seroprevalence for TB was 21.56% (33/153). The majority of seropositive elephants were female (n=30) and from Chitwan National Park (n=29). The occurrence of TB seropositive cases in other more remote national parks suggests TB may be widespread among the captive elephant population of Nepal. Hematology and biochemistry analyses were performed on 13 and 22 seropositive elephants, respectively and, nine elephants from a seronegative TB herd for comparison. Hematology parameters (hemoglobin, packed cell volume, platelet, white blood cells, and erythrocyte sedimentation rate) were comparable between the two groups. Total protein, globulin, and lactate dehydrogenase were significantly higher in seronegative elephants, and bilirubin was significantly higher in seropositive elephants whereas blood urea nitrogen, creatinine, glutamic oxaloacetic transaminase/aspartate aminotransferase (GOT/AST), glutamic pyruvic transaminase/alanine aminotransferase (GPT/ALT), gamma glutamyl transferase (GT), and albumin were not significantly different. The range of biochemical parameters that were significantly different between seropositive and seronegative elephants had narrow ranges. Thus, the potential of these parameters as a direct biomarker for TB diagnosis is limited based on the findings in this study. We recommend including blood parameters in future TB surveillance studies. - Complete Genome Sequence of an mcr-10-Possessing Enterobacter roggenkampii Strain Isolated from a Dog in Japan.
Toyotaka Sato; Masaru Usui; Kazuki Harada; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Shin-Ichi Yokota
Microbiology resource announcements, 10, 30, e0042621, 2021年07月29日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, The complete genome sequence of mcr-10-possessing Enterobacter roggenkampii En37, isolated from a dog in Japan, was determined. mcr-10 was located on a 70,277-bp IncFIB plasmid without any additional antimicrobial resistance genes. - Complete Genome Sequence of an mcr-9-Possessing Enterobacter asburiae Strain Isolated from a Cat in Japan.
Toyotaka Sato; Masaru Usui; Kazuki Harada; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Shin-Ichi Yokota
Microbiology resource announcements, 10, 26, e0028121, 2021年07月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, We report the complete genome sequence of the mcr-9-possessing strain Enterobacter asburiae En30, isolated from a cat in Japan. The genome sequence was obtained by using long- and short-read sequencing. - Interaction of Quinolones Carrying New R1 Group with Mycobacterium leprae DNA Gyrase.
Jong-Hoon Park; Tomoyuki Yamaguchi; Yuki Ouchi; Kentaro Koide; Ruttana Pachanon; Joseph Yamweka Chizimu; Shigetarou Mori; Hyun Kim; Tetsu Mukai; Chie Nakajima; Yasuhiko Suzuki
Microbial drug resistance (Larchmont, N.Y.), 27, 12, 1616, 1623, 2021年06月01日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Background: Leprosy is a chronic infectious disease caused by Mycobacterium leprae and the treatment of choice is ofloxacin (OFX). Specific amino acid substitutions in DNA gyrase of M. leprae have been reported leading to resistance against the drug. In our previous study, WQ-3810, a fluoroquinolone with a new R1 group (6-amino-3,5-difluoropyridin-2-yl) was shown to have a strong inhibitory activity on OFX-resistant DNA gyrases of M. leprae, and the structural characteristics of its R1 group was predicted to enhance the inhibitory activity. Methodology/Principal Finding: To further understand the contribution of the R1 group, WQ-3334 with the same R1 group as WQ-3810, WQ-4064, and WQ-4065, but with slightly modified R1 group, were assessed on their activities against recombinant DNA gyrase of M. leprae. An in silico study was conducted to understand the molecular interactions between DNA gyrase and WQ compounds. WQ-3334 and WQ-3810 were shown to have greater inhibitory activity against M. leprae DNA gyrase than others. Furthermore, analysis using quinolone-resistant M. leprae DNA gyrases showed that WQ-3334 had greater inhibitory activity than WQ-3810. The R8 group was shown to be a factor for the linkage of the R1 groups with GyrB by an in silico study. Conclusions/Significance: The inhibitory effect of WQ compounds that have a new R1 group against M. leprae DNA gyrase can be enhanced by improving the binding affinity with different R8 group molecules. The information obtained by this work could be applied to design new fluoroquinolones effective for quinolone-resistant M. leprae and other bacterial pathogens. - Rapid and simultaneous detection of fluoroquinolone- and macrolide-resistant Campylobacter jejuni/coli in retail chicken meat using CAMpylobacter Express Resistance Array (CAMERA)
Masaru Usui; Sho Tateno; Masanobu Onozaki; Naoaki Misawa; Yasuhiko Suzuki; Yutaka Tamura; Chie Nakajima
Food Control, 123, 107815, 107815, Elsevier BV, 2021年05月, ［査読有り］
研究論文（学術雑誌） - First report of Mycobacterium bovis in wild chacma baboons (Papio ursinus) at the human-wildlife interface area in Zambia.
David Squarre; Joseph Chizimu; Chie Nakajima; John B Muma; Bernard M Hang'ombe; Edgar Simulundu; Wizaso Mwasinga; Jackson Katampi; Paul Fandamu; Victor Mukonka; Yasuhiko Suzuki; Hirofumi Sawa; Hetron M Munang'andu; Griffin Shanungu; Herman M Chambaro; Musso Munyeme
Transboundary and emerging diseases, 69, 3, 1659, 1662, 2021年04月26日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Mycobacterium bovis (M. bovis) causes tuberculosis in mammals and is a major public health threat worldwide. While M. bovis has been reported in humans, domestic and wild ruminants at the human-wildlife-livestock interface area in Zambia, there is paucity of information on the role of primates as reservoir hosts. We screened seven wild chacma baboons (Papio ursinus) for tuberculosis at the human-wildlife interface area in Lochinvar National Park in the Kafue Flats, Zambia. Following necropsy, lung tissue and associated lymph nodes with tuberculous-like lesions collected from four adult male baboons were prepared for Mycobacterium culture. The isolates were initially typed using the Mycobacterium tuberculosis complex-discrimination multiplex PCR assay and further characterized by spoligotyping and 26-loci MIRU-VNTR. Mycobacteria was isolated from all four animals and identified as M. bovis by PCR. On Spoligotyping, all isolates belonged to SB 0120 spoligotype, which is similar to what was previously reported in humans, cattle and Kafue lechwe antelopes in Kafue Flats ecosystem. Furthermore, on MIRU-VNTR typing, the baboon isolates clustered with cattle and Kafue lechwe isolates from the same catchment area. This finding intimates probable cross species transmission of M. bovis in the Kafue Flats ecosystem. Due to the close interaction of baboons and humans at interface areas in Zambia, our results have potential implications for public health. Equally, this finding raises concerns for conservation. - Molecular epidemiology of Mycobacterium bovis in central parts of Malawi.
Thoko Flav Kapalamula; Joseph Chizimu; Lawrence Belotindos; Mwangala Akapelwa; Dipti Shrestha; Mirriam Ethel Nyenje; Musso Munyeme; Bernard Mudenda Hang'ombe; Rajhab Sawasawa Mkakosya; Stephen V Gordon; Chie Nakajima; Yasuhiko Suzuki
Transboundary and emerging diseases, 69, 3, 1577, 1588, 2021年04月26日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Bovine tuberculosis (bTB) is a neglected disease that affects cattle and humans. The burden of bTB is higher in developing countries as compared to industrialized countries. The reasons behind this discrepancy include the fact that bTB control measures, such as testing and slaughter of infected cattle and pasteurization of milk, are not usually practiced in developing countries largely because of their high cost. To improve our understanding of bTB in developing countries molecular typing studies are essential, in particular in terms of transmission dynamics, infection sources, and knowledge of circulating strains of the principal causative agent, Mycobacterium bovis. In this study, we applied a suite of molecular typing techniques encompassing deletion analysis, spoligotyping, and MIRU-VNTR to isolates recovered from samples collected during the routine post-mortem of cattle at the cold-storage abattoir in Lilongwe, Malawi. Out of 63 isolates, 51 (81 %) belonged to the European 1 M. bovis clonal complex. Spoligotyping identified 8 profiles, with SB0131 being the predominant type (56% of isolates). Spoligotypes SB0273 and SB0425 were identified in 14% and 13%, respectively, of the isolates. MIRU-VNTR showed a high discriminatory power of 0.959 and differentiated the 8 spoligotypes to 31 genotypes. The high diversity of M. bovis within the study area suggests the infection has been circulating in the area for a considerable period of time, likely facilitated by the lack of effective control measures. We also observed genetic similarities between isolates from Malawi (this study) to isolates described in previous studies in Zambia and Mozambique, suggesting transmission links in this region. The information provided by this study provides much needed evidence for the formulation of improved bTB control strategies. - Amino Acid Substitution Ser83Ile in GyrA of DNA Gyrases Confers High-Level Quinolone Resistance to Nontyphoidal Salmonella Without Loss of Supercoiling Activity.
Kentaro Koide; Lai Lai San; Ruttana Pachanon; Jong-Hoon Park; Yuki Ouchi; Siriporn Kongsoi; Fuangfa Utrarachkij; Chie Nakajima; Yasuhiko Suzuki
Microbial drug resistance (Larchmont, N.Y.), 27, 10, 1397, 1404, 2021年04月20日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Aims:
Quinolone-resistant nontyphoidal Salmonella having serine replaced by isoleucine at the 83rd amino acid in GyrA (GyrA-Ser83Ile) has recently been found in Asian countries. In this study, we aimed to examine the direct effect of substitution Ser83Ile on DNA gyrase activity and/or resistance to quinolones.
Materials and Methods:
Using 50% of the maximal inhibitory concentrations (IC50s) of quinolones, recombinant wild type (WT) and seven mutant DNA gyrases having amino acid substitutions, including Ser83Ile, were screened for enzymatic activity that causes supercoils in relaxed plasmid DNA and resistance to quinolones.
Results:
Little differences in supercoiling activity were observed between WT and mutant DNA gyrases. By contrast, the IC50s of ciprofloxacin and norfloxacin against GyrA-Ser83Ile/GyrB-WT were 11.6 and 73.3 μg/mL, respectively, which were the highest used against the DNA gyrases examined in this study.
Conclusion:
Ser83Ile in GyrA was shown to confer high-level quinolone resistance to DNA gyrases of nontyphoidal Salmonella, with no loss of supercoiling activity. Salmonella strain carrying GyrA with Ser83Ile may emerge under a high-concentration pressure of quinolones and easily spread even with no selection bias by quinolones. Hence, avoiding the overuse of quinolones is needed to prevent the spread of Salmonella with Ser83Ile in GyrA. - Effectiveness of Fluoroquinolones with Difluoropyridine Derivatives as R1 Groups on the Salmonella DNA Gyrase in the Presence and Absence of Plasmid-Encoded Quinolone Resistance Protein QnrB19.
Ruttana Pachanon; Kentaro Koide; Siriporn Kongsoi; Nami Ajima; Thoko Flav Kapalamula; Chie Nakajima; Orasa Suthienkul; Yasuhiko Suzuki
Microbial drug resistance (Larchmont, N.Y.), 27, 10, 1412, 1419, 2021年04月09日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Aims:
WQ-3810 has strong inhibitory activity against Salmonella and other fluoroquinolone-resistant pathogens. The unique potentiality of this is attributed to 6-amino-3,5-difluoropyridine-2-yl at R1 group. The aim of this study was to examine WQ-3810 and its derivatives WQ-3334 and WQ-4065 as the new drug candidate for wild-type Salmonella and that carrying QnrB19.
Materials and Methods:
The half maximal inhibitory concentrations (IC50s) of WQ-3810, WQ-3334 (Br atom in place of methyl group at R8), and WQ-4065 (6-ethylamino-3,5-difluoropyridine-2-yl in place of 6-amino-3,5-difluoropyridine-2-yl group at R1) in the presence or absence of QnrB19 were assessed by in vitro DNA supercoiling assay utilizing recombinant DNA gyrase and QnrB19.
Results:
IC50s of WQ-3810, WQ-3334, and WQ-4065 against Salmonella DNA gyrase were 0.031 ± 0.003, 0.068 ± 0.016, and 0.72 ± 0.39 μg/mL, respectively, while QnrB19 increased IC50s of WQ-3810, WQ-3334, and WQ-4065 to 0.44 ± 0.05, 0.92 ± 0.34, and 9.16 ± 2.21 μg/mL, respectively.
Conclusion:
WQ-3810 and WQ-3334 showed stronger inhibitory activity against Salmonella Typhimurium DNA gyrases than WQ-4065 even in the presence of QnrB19. The results suggest that 6-amino-3,5-difluoropyridine-2-yl group at R1 is playing an important role and WQ-3810 and WQ-3334 to be good candidates for Salmonella carrying QnrB19. - Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines.
Lawrence Belotindos; Marvin Villanueva; Joel Miguel Jr; Precious Bwalya; Tetsuya Harada; Ryuji Kawahara; Chie Nakajima; Claro Mingala; Yasuhiko Suzuki
Antibiotics (Basel, Switzerland), 10, 4, 2021年04月09日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Antimicrobial resistance to quinolones, which constitutes a threat to public health, has been increasing worldwide. In this study, we investigated the prevalence of quinolone-resistant determinants in Escherichia coli not susceptible to quinolones and isolated from food-producing animals and food derived from them, in the Philippines. A total of 791 E. coli strains were isolated in 56.4% of 601 beef, chicken, pork, egg, and milk samples, as well as environmental, cloacal, and rectal swab-collected samples from supermarkets, open markets, abattoirs, and poultry, swine, and buffalo farms. Using the disc diffusion method, it was determined that 78.6% and 55.4% of the isolates were resistant to at least one antimicrobial and multiple drugs, respectively. In 141 isolates not susceptible to quinolones, 115 (81.6%) harbored quinolone-resistant determinants and had mutations predominantly in the quinolone-resistance determining regions (QRDRs) of gyrA and parC. Plasmid-mediated, quinolone resistance (PMQR) and Qnr family (qnrA1, qnrB4, and qnrS1) genes were detected in all isolates. Forty-eight sequence types were identified in isolates harboring mutations in QRDR and/or PMQR genes by multilocus sequence typing analysis. Moreover, 26 isolates harboring mutations in QRDR and/or PMQR genes belonged mostly to phylogroup B1 and Enteroaggregative E. coli. In conclusion, a high prevalence of E. coli was found in food-producing animals and products derived from them, which could potentially spread high-risk clones harboring quinolone-resistance determinants. - Evaluation of Xpert MTB/RIF with microscopy and culture for the diagnosis of tuberculosis in a referral laboratory in Nepal.
Bhagwan Maharjan; Jeewan Thapa; Dhirendra Kumar Shah; Bhabana Shrestha; Korkut Avsar; Yasuhiko Suzuki; Chie Nakajima
Japanese journal of infectious diseases, 74, 6, 517, 521, 2021年03月31日, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, Sputum microscopy and Xpert MTB/RIF are the primary rapid diagnostic methods for tuberculosis (TB) in Nepal. Disagreements among Xpert, microscopy, and culture, for example, cases with Xpert positive and microscopy negative, were frequently observed in Nepal including in our reference laboratory. The objective of this study was to compare the effectiveness of Xpert with culture and microscopy for TB diagnosis in Nepal. A total of 125 TB suspected sputum samples were processed for Xpert, microscopy, and culture. The Xpert results when compared with culture showed 100% sensitivity and 97.4% specificity with an excellent agreement (kappa = 0.96), whereas microscopy showed the sensitivity and specificity of 43.2% and 98.7%, respectively, with a moderate agreement (kappa = 0.4). The sensitivity and specificity of microscopy, when compared with Xpert, were 43.5% and 100%, respectively. The majority of Xpert positive samples of a medium MTB detection and all samples of low and very low MTB detection were missed by microscopy. Our study showed that Xpert MTB/RIF is a reliable tool for the diagnosis and management of TB in Nepal. Because of its high cost and sustainability, alternative simple and rapid diagnostic methods with a similar efficiency would be helpful for TB control in Nepal. - Antimicrobial Resistance and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Slaughtered Pigs and Pork in the Central Region of Thailand.
Wimonrat Tanomsridachchai; Kanjana Changkaew; Ruchirada Changkwanyeun; Watsawan Prapasawat; Apiradee Intarapuk; Yukari Fukushima; Nattapong Yamasamit; Thoko Flav Kapalamula; Chie Nakajima; Orasa Suthienkul; Yasuhiko Suzuki
Antibiotics (Basel, Switzerland), 10, 2, 2021年02月19日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Methicillin-resistant Staphylococcus aureus (MRSA) have been a major public health concern in humans. Among MRSA, livestock-associated (LA)-MRSA strains have always been associated with exposure to livestock or their products and have emerged in different countries globally. Although studies have identified LA-MRSA from healthy pigs and pork in Thailand, prevalence in slaughtered pigs is still unknown. In addition, there are few reports on the epidemiology and molecular characteristics of LA-MRSA in Thailand. Hence, this is the first report investigating the epidemiology and molecular characteristics of MRSA in individual slaughtered pigs and pork in Thailand. A total of 204 nasal swab and 116 retailed pork samples were collected from three slaughterhouses and four fresh markets, respectively. Individual samples were used for screening for MRSA and obtained isolates were examined for drug- resistance profiling for 12 antimicrobial agents of 10 drug classes. In addition, SCCmec typing and multi-locus sequence typing were conducted to obtain genotype profiles. MRSA were isolated from 11 and 52 nasal swab and pork samples, respectively. The prevalence was significantly higher in the pork than in the nasal swab samples (p-value < 0.05). A high prevalence of ST9-SCCmecIX and ST398-SCCmecV with high-level antimicrobial resistance from markets and slaughterhouses indicated the spreading of MRSA with these genotypes in the Thai swine processing chains and suggested the need for further investigation to determine a control. - Genetic characterization of coliform bacterial isolates from environmental water in Thailand.
Risa Tsunoda; Masaru Usui; Chie Tagaki; Akira Fukuda; Chanchai Boonla; Wilai Anomasiri; Nop Sukpanyatham; Mwangala Lonah Akapelwa; Chie Nakajima; Yutaka Tamura; Yasuhiko Suzuki
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 27, 5, 722, 728, 2021年01月16日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, INTRODUCTION: In contrast to the study in other part of the world, information about characteristics of plasmids carrying antimicrobial resistance genes (ARGs) in Enterobacteriaceae derived from environmental water in tropical Asian countries including Thailand is limited. This study, therefore, aimed to gain insight into genetic information of antimicrobial resistance in environmental water in Thailand. METHODS: Coliform bacteria were isolated from environmental water collected at 20 locations in Thailand and identified. Then, susceptibility profiles to ampicillin, cefazoline, cefotaxime, kanamycin, ciprofloxacin, sulfamethoxazole, tetracycline, and nalidixic acid were assessed. In addition, antimicrobial resistant genes integrons, and replicon types were analyzed. And furthermore, plasmids carrying blaTEM and tetM were identified by S1-PFGE analysis and confirmed transmissibility by transconjugation experiments. RESULTS: In 130 coliform bacteria isolated, 89 were resistant to cefazoline while 41 isolates were susceptible. Cefazoline-resistant coliform bacteria were found to be significantly resistant to cefotaxime and tetracycline as compared to susceptible isolates. Hence, blaTEM and tetM correlating with β-lactam antibiotics and tetracycline, respectively, were analyzed found to co-localize on the IncFrepB plasmids in isolates from pig farms' wastewater by S1-PFGE analysis. And furthermore, transmissibility of the plasmids was confirmed. CONCLUSIONS: Results obtained in this study suggested that ARGs in coliform bacteria may have been spreading on the farm via IncFrepB plasmids. Hence, appropriate use of antimicrobials and good hygiene management on the farm are required to prevent the emergence and spread of resistant bacteria. - Aerobic Composting and Anaerobic Digestion Decrease the Copy Numbers of Antibiotic-Resistant Genes and the Levels of Lactose-Degrading Enterobacteriaceae in Dairy Farms in Hokkaido, Japan.
Satoshi Katada; Akira Fukuda; Chie Nakajima; Yasuhiko Suzuki; Takashi Azuma; Ayaka Takei; Hideshige Takada; Eiryu Okamoto; Toshihide Kato; Yutaka Tamura; Masaru Usui
Frontiers in microbiology, 12, 737420, 737420, 2021年, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - ERRATUM: Rapid and Simple Detection of Isoniazid-Resistant Mycobacterium tuberculosis Utilizing a DNA Chromatography-Based Technique.
Takuya Kodera; Tomoyuki Yamaguchi; Yukari Fukushima; Kumi Kobayashi; Yutaka Takarada; Joseph Yamweka Chizimu; Chie Nakajima; Eddie Samuneti Solo; Patrick Saili Lungu; Mitsuo Kawase; Yasuhiko Suzuki
Japanese journal of infectious diseases, 74, 4, 385, 385, 2021年, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, Volume 74, no.3, p.214-219, 2021. Page 214, affiliation "1TBA Co., LTD, Sendai; 2Hokkaido University Research Center for Zoonosis Control, Sapporo; 3Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo; 4Zambia National Public Health Institute, Ministry of Health, Lusaka, Zambia; 5Department of Pathology and Microbiology, University Teaching Hospital Ministry of Health, Lusaka, Zambia; and 6Ministry of Health, Ndeke House, Lusaka, Zambia." should read "1TBA Co., LTD, Sendai, Japan; 2Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; 3Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo, Japan; 4Zambia National Public Health Institute, Ministry of Health, Lusaka, Zambia; 5Department of Pathology and Microbiology, University Teaching Hospital Ministry of Health, Lusaka, Zambia; and 6Ministry of Health, Ndeke House, Lusaka, Zambia". - Development of a loop-mediated isothermal amplification (LAMP) method for specific detection of Mycobacterium bovis.
Thoko Flav Kapalamula; Jeewan Thapa; Mwangala Lonah Akapelwa; Kyoko Hayashida; Stephen V Gordon; Bernard Mudenda Hang' Ombe; Musso Munyeme; Eddie Samuneti Solo; Precious Bwalya; Mirriam Ethel Nyenje; Aki Tamaru; Yasuhiko Suzuki; Chie Nakajima
PLoS neglected tropical diseases, 15, 1, e0008996, 2021年01月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Bovine tuberculosis (TB) caused by Mycobacterium bovis is a significant health threat to cattle and a zoonotic threat for humans in many developing countries. Rapid and accurate detection of M. bovis is fundamental for controlling the disease in animals and humans, and for the proper treatment of patients as one of the first-line anti-TB drug, pyrazinamide, is ineffective against M. bovis. Currently, there are no rapid, simplified and low-cost diagnostic methods that can be easily integrated for use in many developing countries. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for specific identification of M. bovis by targeting the region of difference 4 (RD4), a 12.7 kb genomic region that is deleted solely in M. bovis. The assay's specificity was evaluated using 139 isolates comprising 65 M. bovis isolates, 40 M. tuberculosis isolates, seven M. tuberculosis complex reference strains, 22 non-tuberculous mycobacteria and five other bacteria. The established LAMP detected only M. bovis isolates as positive and no false positives were observed using the other mycobacteria and non-mycobacteria tested. Our LAMP assay detected as low as 10 copies of M. bovis genomic DNA within 40 minutes. The procedure of LAMP is simple with an incubation at a constant temperature. Results are observed with the naked eye by a color change, and there is no need for expensive equipment. The established LAMP can be used for the detection of M. bovis infections in cattle and humans in resource-limited areas. - Emergence of the Novel Aminoglycoside Acetyltransferase Variant aac(6')-Ib-D179Y and Acquisition of Colistin Heteroresistance in Carbapenem-Resistant Klebsiella pneumoniae Due to a Disrupting Mutation in the DNA Repair Enzyme MutS.
Toyotaka Sato; Takayuki Wada; Suguru Nishijima; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Satoshi Takahashi; Shin-Ichi Yokota
mBio, 11, 6, 2020年12月22日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Amikacin and colistin are effective against carbapenem-resistant Klebsiella pneumoniae In 2017, we successively isolated three carbapenem-resistant K. pneumoniae isolates (ST967) from a patient with chronic renal failure in Japan. The first (SMKP01, sputum, day 0) and second (SMKP02, blood, day 14) strains were resistant to most antimicrobials tested but still susceptible to amikacin (MICs of 4 and 0.5 mg/liter, respectively) and colistin (MIC of 0.5 mg/liter for both). The third strain (SMKP03, blood, day 51) was not susceptible to amikacin (MIC, 32 mg/liter), and its MIC for colistin varied (0.5 to 8 mg/liter). Whole-genome sequencing of SMKP01 revealed that 17 of 20 antimicrobial resistance genes, including qnrB91 (a novel qnrB2 variant) and aac(6')-Ib-cr, were located on an 86.9-kb IncFII-IncQ plasmid. The qnrB91 conferred greater fluoroquinolone resistance than qnrB2 SMKP03 aac(6')-Ib-cr that possessed a gene mutation that resulted in an R102W substitution, namely, aac(6')-Ib-D179Y, made a greater contribution to amikacin resistance than did aac(6')-Ib-cr SMKP03 harbored a nonsense mutation in mutS, which encodes a DNA repair enzyme. Introduction of this mutation into SMKP01 (SMKP01mutSA307T) resulted in a dramatic increase (>58-fold) in the frequency of spontaneous amikacin-resistant mutants relative to SMKP01, and the substantial mutants possessed aac(6')-Ib-D179Y SMKP01mutSA307T exhibited an unstable MIC for colistin (0.5 to 8 mg/liter). The results demonstrate that a disruptive mutation in MutS, arising during the clinical course of an infection, created a platform for the acquisition of amikacin nonsusceptibility and colistin heteroresistance in multidrug-resistant K. pneumoniae, mediated by the elevated frequency of spontaneous mutations.IMPORTANCE The emergence of multidrug resistance in pathogens such as Klebsiella pneumoniae is of great clinical concern. Antimicrobial resistance sometimes arises during the course of an infection. Although many studies have reported the emergence of antimicrobial resistance and novel antimicrobial resistance genes in the clinical isolates, the identity of the bacterial factor(s) that generate this emergence is still unclear. We report that a disruptive mutation in MutS, arising during the clinical course of an infection, created a context for the acquisition of colistin resistance and the emergence of a novel variant of the amikacin resistance gene in multidrug-resistant K. pneumoniae via an increase in the frequency of spontaneous mutation. This observation is important for understanding how K. pneumoniae develops multidrug resistance during infection and could potentially lead to new antimicrobial treatments for high-risk pathological microbes. - Drug-resistant Mycobacterium tuberculosis and its genotypes isolated from an outbreak in western Thailand.
Janisara Rudeeaneksin; Benjawan Phetsuksiri; Chie Nakajima; Supranee Bunchoo; Krairerk Suthum; Nattakan Tipkrua; Yukari Fukushima; Yasuhiko Suzuki
Transactions of the Royal Society of Tropical Medicine and Hygiene, 115, 8, 886, 895, 2020年12月15日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Multidrug-resistant TB (MDR-TB) outbreaks have occurred in the Thamaka district, Kanchanaburi province in Thailand. METHODS: Seventy-two isolates, which included 7% mono-, 30.6% MDR and extensively drug-resistant TB (XDR-TB), were genotyped by spoligotyping, mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) and single nucleotide polymorphism genotyping, and their drug resistance was analysed. RESULTS: The spoligotyping results showed that Beijing spoligo-international type (SIT)1 was predominant (n=38; 52.8%) while the remaining were non-Beijing sublineages (n=34). The MIRU-VNTR analysis showed that Beijing isolates, most of which belonged to the modern type (n=37), formed 5 clusters and 13 individual patterns. In katG, only mutation Ser315Thr was identified. In rpoB, Ser531Leu was predominant, except for His526Arg and Leu533Pro, which were found in two isolates. A cluster of 14 Beijing strains contained these common mutations and shared the MIRU-VNTR genotype with isolates in the Thamaka district that had spread previously. Two U SIT523 isolates contained the mutations A1400G in rrs and Asp94Gly in gyrA genes, indicating a spread of XDR-TB. CONCLUSIONS: Most mutations were associated with drug resistance and the specific MDR Beijing and XDR-TB in U SIT523 isolates remain. This genotyping is a key tool for tracking TB transmission in the Thamaka district of Thailand. - Characterization of Vibrio parahaemolyticus strains isolated from clinically asymptomatic seafood workers.
Kaknokrat Chonsin; Neunghatai Supha; Chie Nakajima; Yasuhiko Suzuki; Orasa Suthienkul
FEMS microbiology letters, 368, 1, 2020年12月15日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Vibrio parahaemolyticus (VP) is a major cause of gastroenteritis outbreaks in Thailand and other countries due to the consumption of contaminated and undercooked seafood. However, there have been few reports of the molecular epidemiology of VP isolates from asymptomatic seafood handlers. Here, we report the phenotypic and genetic characterization of 61 VP isolates obtained from asymptomatic workers in two seafood processing plants. We found 24 O:K serotypes of which O11:KUT, O1:KUT and O3:KUT were the dominant serotypes. Analysis by PCR showed 12 isolates harbored either tdh or trh genes with the potential to be pathogenic VP strains. The presence of T3SS2α and T3SS2β genes was correlated with the presence of tdh and trh, respectively. Four tdh+ isolates were positive for pandemic marker. In this study, VP isolates were commonly resistant to ampicillin, cephazolin, fosfomycin and novobiocin. Phylogenetic analysis of VP1680 loci in 35 isolates from 17 asymptomatic workers, six gastroenteritis patients, seven environmental samples and five genomes from a database showed 22 different alleles. Gene VP1680 was conserved in tdh+ isolates and pandemic strains, that of trh + isolates was diverse. Asymptomatic workers carrying VP were the most likely source of contamination, which raises concerns over food safety in seafood processing plants. - Sitafloxacin has a potent activity for eradication of extended spectrum β-lactamase-producing fluoroquinolone-resistant Escherichia coli forming intracellular bacterial communities in uroepithelial cells.
Yoshiki Hiyama; Toyotaka Sato; Satoshi Takahashi; Soh Yamamoto; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Shin-Ichi Yokota; Naoya Masumori
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 26, 12, 1272, 1277, 2020年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, INTRODUCTION: Eradication of asymptomatic bacteriuria (ASB) before urological procedures is important to reduce the risk for infectious complications after surgery. However, the appropriate regimen for antimicrobial treatment has not been fully determined. We experienced continuous (over 10 months) isolation of extended spectrum β-lactamase (ESBL)-producing fluoroquinolone-resistant Escherichia coli from urine of an asymptomatic patient. The four isolates obtained (SMESC1 to 4) were international high-risk clones of O25b:H4-ST131-H30R, and originated from one strain, as revealed by the whole genome sequences. Although the patient received meropenem (MEPM) and fosfomycin (FOM), to which the strains were susceptible before the urological procedures, they could not be eradicated. METHODS: To explore the reason for the continuous isolation even after MEPM and FOM administration, antimicrobial killing of adherent and/or intracellular bacterial communities (IBC) formed by coculture of the E. coli cells and T24 bladder epithelial cells were examined. RESULTS: FOM and levofloxacin did not decrease viable E. coli cells compared with gentamicin. MEPM partly decreased them, and sitafloxacin (STFX) decreased them most potently. These observations indicate that E. coli can survive in the urinary tract under antimicrobial administration, and some antimicrobials such as FOM and MEPM cannot eradicate E. coli in uroepithelial cells. Adhesion on urinary epithelial cells and/or IBC formation might result in continuous isolation from the urinary tract and recurrence of ASB and urinary tract infections. CONCLUSIONS: The present study suggests that STFX is a promising optional agent for the eradication of ESBL-producing fluoroquinolone-resistant E. coli in the urinary tract before urological procedures. - Molecular analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis isolates from Nepal.
Dipti Shrestha; Bhagwan Maharjan; Nan Aye Thida Oo; Norikazu Isoda; Chie Nakajima; Yasuhiko Suzuki
Tuberculosis (Edinburgh, Scotland), 125, 101985, 101985, 2020年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Mutation in rpsL (encoding ribosomal protein S12), rrs (encoding 16S ribosomal RNA) and gidB (encoding 7-methylguanosine methyltransferase) are associated with resistance to streptomycin (STR), which is used for the treatment of multi-drug resistant tuberculosis (MDR-TB) in Nepal. The aim of our study is to analyze the correlation between mutations in the target genes and STR-resistance in 197 Mycobacterium tuberculosis (MTB) isolates from Nepal. Mutations in rpsL was harbored by 65.9% of isolates, in which the most common mutation in rpsL is caused by K43R (58.8%) and were significantly associated with Beijing genotype (P < 0.001). About 13.2% of isolates harbored mutations in two highly mutable regions of rrs, the 530 loop and the 912 region. About 13.2% of gidB mutants do not show any mutation in rpsL and rrs, which might suggest the role of gidB mutations in STR-resistance in MTB. In addition, 5.6% of isolates do not show any mutations in three genes examined, suggesting the involvement of other mechanism in STR-resistance in MTB. Our findings can be implemented for the establishment of molecular STR-susceptibility testing, in which tuberculosis can be treated with appropriate drugs and can improve control strategies for DR-TB. - Interaction of the plasmid-encoded quinolone resistance protein QnrB19 with Salmonella Typhimurium DNA gyrase.
Ruttana Pachanon; Kentaro Koide; Siriporn Kongsoi; Chie Nakajima; Thoko Flav Kapalamula; Orasa Suthienkul; Yasuhiko Suzuki
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 26, 11, 1139, 1145, 2020年11月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Plasmid-encoded quinolone resistance protein Qnr is an important factor in bacterial resistance to quinolones. Qnr interacts with DNA gyrase and reduces susceptibility to quinolones. The gene qnr likely spreads rapidly among Enterobacteriaceae via horizontal gene transfer. Though the vast amounts of epidemiological data are available, molecular details of the contribution of QnrB19, the predominant Qnr in Salmonella spp., to the acquisition of quinolone resistance has not yet been understood well. OBJECTIVE: We aimed to examine the role of QnrB19 in quinolone resistance acquisition using recombinant Salmonella Typhimurium DNA gyrases and QnrB19. MATERIALS AND METHODS: Recombinant QnrB19 was expressed in E. coli and purified by Ni-NTA agarose column chromatography. DNA supercoiling activities of recombinant Salmonella Typhimurium DNA gyrase were assessed with or without QnrB19 under the existence of three quinolones to measure IC50s, the concentration of each quinolone required for 50% inhibition in vitro. RESULTS: The IC50s of norfloxacin, ciprofloxacin and nalidixic acid against DNA gyrases were measured to be 0.30, 0.16 and 17.7 μg/mL, respectively. The addition of QnrB19 increased the IC50s of norfloxacin and ciprofloxacin to be 0.81 and 0.48 μg/mL, respectively, where no effect of QnrB19 was observed on the IC50 of nalidixic acid. CONCLUSION: QnrB19 was shown for the first time in vitro to have ability to grant non-classical quinolone resistance to S. Typhimurium DNA gyrase. Structural insight on quinolones in this study may contribute to investigate drugs useful for preventing the spread of plasmid carrying PMQR along with other factors associating with antimicrobial resistance in S. Typhimurium and other bacteria. - Rapid and simple detection of isoniazid resistant Mycobacterium tuberculosis utilizing DNA chromatography based technique.
Takuya Kodera; Tomoyuki Yamaguchi; Yukari Fukushima; Kumi Kobayashi; Yutaka Takarada; Joseph Yamweka Chizimu; Chie Nakajima; Eddie Samuneti Solo; Patrick Saili Lungu; Mitsuo Kawase; Yasuhiko Suzuki
Japanese journal of infectious diseases, 74, 3, 214, 219, 2020年10月30日, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, Despite the availability of anti-tuberculosis drugs, treatment of tuberculosis has been complicated by drug resistant tuberculosis. Early detection of drug resistance make early appropriate treatment possible. However, the available tools are mainly for rifampicin resistance and the existing isoniazid resistance detection method is expensive, highly technical and complicated to maintain to make them unsustainable for use in developing nations. This study aimed to develop a simple, rapid and low-cost diagnostic kit for isoniazid resistant tuberculosis using single-stranded tag hybridization method targeting an isoniazid resistance conferring mutation. Specificity and sensitivity were assessed by utilizing DNA extracted from 49 isoniazid resistant and 41 susceptible Mycobacterium tuberculosis clinical isolates cultured in Mycobacterial Growth Indicator Tubes. Positive signals were observed at mutant and wildtype lines with the sensitivity and specificity of 100% comparing to sanger sequencing results. In contrast, no positive signal was observed with non-tuberculosis mycobacteria. And the detection limit of this method was supposed to be 103 CFU or less. The STH-PAS method for isoniazid resistant M. tuberculosis detection developed in this study offers a better alternative for conventional phenotypic isoniazid resistance determination which will be of both clinical and epidemiological significance in resource limited nations. - Characterization of Mycobacterium tuberculosis genotypes and their correlation to multidrug resistance in Lusaka, Zambia.
Eddie Samuneti Solo; Yasuhiko Suzuki; Trevor Kaile; Precious Bwalya; Patrick Lungu; Joseph Yamweka Chizimu; Yogendra Shah; Chie Nakajima
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 102, 489, 496, 2020年10月12日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVES: The burden of multidrug-resistant tuberculosis (MDR-TB) has been reported to be increasing in Zambia. The reasons for the increase are still unclear. This study determined the diversity of Mycobacterium tuberculosis genotypes among isolates in Lusaka, the capital city, and investigated their association with MDR-TB. METHODS: Spoligotyping, large sequence polymorphism (LSP) analysis, and sequencing of MDR associated genes were performed on a total of 274 M. tuberculosis clinical isolates stored at the University Teaching Hospital from 2013 to 2017. Of these, 134 were MDR-TB while 126 were pan-susceptible. RESULTS: Spoligotyping showed the LAM family as the most predominant genotype (149/274, 54.4%) followed by the CAS family (44/274, 16.1%), T family (39/274, 14.2%), and minor proportions of X, S, Harleem, EAI and Beijing spoligofamilies were identified. Three M. bovis isolates were also observed. Among those, CAS1-Kili (SIT 21) and LAM1 (SIT 20) subfamilies showed a propensity for MDR-TB with p = 0.0001 and p = 0.001, respectively. CONCLUSIONS: This phenomenon might explain the future increase in the MDR-TB burden caused by specific lineages in Zambia. Therefore, it is recommended that the National TB control program in the country complements conventional control strategies with molecular analysis for monitoring and surveillance of MDR-TB epidemiology. - Rapid detection of rifampicin-resistant Mycobacterium tuberculosis, based on isothermal DNA amplification and DNA chromatography.
Yutaka Takarada; Takuya Kodera; Kumi Kobayashi; Chie Nakajima; Mitsuo Kawase; Yasuhiko Suzuki
Journal of microbiological methods, 177, 106062, 106062, 2020年10月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Rapid and easy detection of nucleotide point mutations in bacterial pathogens associated with drug resistance is essential for the proper use of antimicrobials. Here, we developed a rapid and simple method for the detection of mutations using Loop-mediated isothermal amplification (LAMP) combined with the single-tag hybridization (STH) chromatographic printed array strips (PAS) method. This procedure is able to detect four mutations (C1349 T, A1295C, G1303 T, A1304 T) in Rifampicin Resistance Determining Region (RRDR) of rifampicin-resistant Mycobacterium tuberculosis (RR-TB), simultaneously. LAMP reactions contained a LAMP primer and eight allele-specific primers for each mutation. The allele-specific primers products were detected by nucleic acid chromatography using PAS. Four detection lines were detected there, one of which was detected at different positions depend on the wild type and the mutant type. We carried out the four mutations detection using 31 genomic DNA (2 A1295T, 1 G1303 T, 6 A1304 T, 22 C1349 T) from clinical isolate. The mutations have been confirmed by sequence analysis. The detection results were completely consistent with the sequence analysis. In the present study, four mutations could be detected, but only 60% of RR-TB could be detected with these four. It is expected that the detection rate will increase by adding more mutant primers. The combined LAMP and STH chromatographic PAS method is a simple and rapid method for detecting point mutations in clinical isolates as a point-of-care testing (POCT) technique. In addition, it does not require special equipment and can meet the demand in areas where drug-resistant bacteria are endemic, such as developing countries. - Elucidating the Structural Requirement of Uridylpeptide Antibiotics for Antibacterial Activity.
Yuma Terasawa; Chisato Sataka; Toyotaka Sato; Kazuki Yamamoto; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Akira Katsuyama; Takanori Matsumaru; Fumika Yakushiji; Shin-Ichi Yokota; Satoshi Ichikawa
Journal of medicinal chemistry, 63, 17, 9803, 9827, 2020年09月10日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, The synthesis and biological evaluation of analogues of uridylpeptide antibiotics were described, and the molecular interaction between the 3'-hydroxy analogue of mureidomycin A (3'-hydroxymureidomycin A) and its target enzyme, phospho-MurNAc-pentapeptide transferase (MraY), was analyzed in detail. The structure-activity relationship (SAR) involving MraY inhibition suggests that the side chain at the urea-dipeptide moiety does not affect the MraY inhibition. However, the anti-Pseudomonas aeruginosa activity is in great contrast and the urea-dipeptide motif is a key contributor. It is also suggested that the nucleoside peptide permease NppA1A2BCD is responsible for the transport of 3'-hydroxymureidomycin A into the cytoplasm. A systematic SAR analysis of the urea-dipeptide moiety of 3'-hydroxymureidomycin A was further conducted and the antibacterial activity was determined. This study provides a guide for the rational design of analogues based on uridylpeptide antibiotics. - Emergence of vancomycin- and teicoplanin-resistant Enterococcus faecium via vanD5-harbouring large genomic island.
Toyotaka Sato; Takayuki Wada; Masaaki Shinagawa; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Satoshi Takahashi; Shin-Ichi Yokota
The Journal of antimicrobial chemotherapy, 75, 9, 2411, 2415, 2020年09月01日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Treatment of VRE is of clinical concern. While certain numbers of vanD-type VRE have been isolated, only two vanD5-harbouring Enterococcus faecium isolates have been reported in Canada and Japan. METHODS: We report the isolation of vanD5-type E. faecium and the first ever determination of the whole-genome sequence to investigate the possible mechanisms of the acquisition of the vanD5 gene cluster in E. faecium. RESULTS: Two vanD5-harbouring vancomycin-resistant E. faecium were isolated from the skin (SMVRE19) and faeces (SMVRE20) of a patient with a skin ulcer in Japan. The isolates exhibited vancomycin and teicoplanin MIC values of 128 mg/L, whilst the previous isolates of vanD5-harbouring E. faecium were only resistant to vancomycin. SMVRE19 and SMVRE20 were clones related to ST18, which is also seen in vanA- and vanB-type VRE. These isolates harboured an insertion element, ISEfm1, in the ddl gene, similar to a previously described teicoplanin-resistant vanD3-type E. faecium. The vanD5 gene cluster was integrated into the SMVRE20 chromosome as a part of a large genomic island (approximately 127 kb), similar to other recently spreading vanD variants in the Netherlands. The genomic island shared the greatest similarity with a part of the Blautia coccoides genome sequence, except for the region surrounding the vanD gene cluster. CONCLUSIONS: This study reports that emergence of vancomycin- and teicoplanin-resistant vanD5-type E. faecium occurred via acquisition of the vanD5 cluster and ISEfm1 insertion into ddl. Considering the genetic similarity between the various VRE strains, the current study should serve as a warning against the spread of vanD5-type VRE. - Mutations in rpoB and katG genes and the inhA operon in multidrug-resistant Mycobacterium tuberculosis isolates from Zambia.
Eddie S Solo; Chie Nakajima; Trevor Kaile; Precious Bwalya; Grace Mbulo; Yukari Fukushima; Sylvia Chila; Nanthan Kapata; Yogendra Shah; Yasuhiko Suzuki
Journal of global antimicrobial resistance, 22, 302, 307, 2020年09月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVES: It is established that resistance to rifampicin (RIF) in 90% of RIF-resistant Mycobacterium tuberculosis isolates is attributable to point mutations in the rpoB gene, whilst 50-95% of M. tuberculosis resistance to isoniazid (INH) is caused by mutations in the katG gene. However, the patterns and frequencies of mutations vary by geographical region. In Zambia, the genetic mechanisms of resistance of M. tuberculosis to RIF and INH were unreported before this study. METHODS: Using gene sequencing, the rpoB, katG and inhA genes of 99 multidrug-resistant M. tuberculosis (MDR-TB) and 49 pan-susceptible M. tuberculosis isolates stored at a tuberculosis reference laboratory from 2013 to 2016 were analysed and were compared with published profiles from other African countries. RESULTS: Of the 99 MDR-TB isolates, 95 (96.0%) carried mutations in both rpoB and katG. No mutations were detected among the pan-susceptible isolates. The most common mutations among RIF- and INH-resistant isolates were in codon 531 of the rpoB gene (55.6%; 55/99) and codon 315 of the katG gene (94.9%; 94/99), respectively. Distinctly, katG mutations were predominantly high among Zambian isolates (96.0%) compared with other countries in the region. CONCLUSION: Resistance-associated mutations to RIF and INH circulating in Zambia are similar to those reported globally, therefore these data validate the applicability of molecular diagnostic tools in Zambia. However, katG mutations were predominantly high among M. tuberculosis isolates in this study compared with other regional countries and might distinguish cross-boundary transmission of MDR-TB from other African nations. - Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis.
Benjawan Phetsuksiri; Janisara Rudeeaneksin; Sopa Srisungngam; Supranee Bunchoo; Wiphat Klayut; Chie Nakajima; Shigeyugi Hamada; Yasuhiko Suzuki
Japanese journal of infectious diseases, 73, 4, 272, 277, 2020年07月22日, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, The diagnosis of tuberculosis (TB) in endemic countries is challenging due to high caseloads and limited resources. A simple and cost-effective diagnostic test for the rapid detection of Mycobacterium tuberculosis (M. tuberculosis) in clinical specimens is crucially needed. We evaluated the performance of an in-house assay based on loop-mediated isothermal amplification (LAMP) targeting the M. tuberculosis 16S ribosomal RNA (rRNA) gene for the diagnosis of TB in Thailand. A total of 252 sputum samples from suspected cases of pulmonary TB were analyzed. The sensitivity of LAMP was 99.04% (103/104; 95% confidence interval [CI]: 94.76-9.98%) and 72.73% (16/22; 95% CI: 49.78-89.27%) for smear-positive and smear-negative samples with TB-culture positivity, respectively. LAMP detected 20.69% (24/116) of TB culture negative samples but all those were positive by conventional polymerase chain reaction (PCR). The sensitivity of LAMP was higher than that of sputum microscopy while the performance of LAMP was similar to PCR. None of the samples positive for non-tuberculous mycobacteria by culture and PCR were positive by LAMP. Compared to TB culture, the positive predictive value (PPV), negative predictive value (NPV), and kappa coefficient of LAMP were 83.22%, 88.33%, and 0.75 respectively. Based on the diagnostic performance, we propose that LAMP would be suitable as a potential diagnostic test for rapid TB diagnosis in resource-limited laboratory settings. - Campylobacter upsaliensis isolated from a giant hepatic cyst.
Yasuo Ohkoshi; Toyotaka Sato; Hiromi Murabayashi; Kohei Sakai; Yasunari Takakuwa; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Shin-Ichi Yokota
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 26, 7, 752, 755, 2020年07月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Campylobacter upsaliensis is an enteropathogenic bacterium in animals, and is also rarely isolated from humans, where it can cause enteritis and bacteremia. This report describes the first case of isolation of C. upsaliensis from an infected giant hepatic cyst. This bacterium could not be cultured from abscess punctuate in a usual Campylobacter-selection medium (charcoal cefoperazone deoxycholate agar medium), because of high concentration of cefoperazone as a selection agent. It could not identified by matrix-assisted laser desorption ionization-time of flight mass spectrum. Rather, it was identified as C. upsaliensis by whole genome sequencing, including by multilocus sequence typing. - Pyrimidine Analogues as a New Class of Gram-Positive Antibiotics, Mainly Targeting Thymineless-Death Related Proteins.
Chihiro Oe; Hironori Hayashi; Kazushige Hirata; Kumi Kawaji; Fusako Hashima; Mina Sasano; Maaya Furuichi; Emiko Usui; Makoto Katsumi; Yasuhiko Suzuki; Chie Nakajima; Mitsuo Kaku; Eiichi N Kodama
ACS infectious diseases, 6, 6, 1490, 1500, 2020年06月12日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Multidrug-resistant (MDR) bacteria are widespread throughout the world and pose an increasingly serious threat to human and animal health. Besides implementing strict measures to prevent improper antibiotic use, it remains essential that novel antibiotics must be developed. These antibiotics need to exert their activity via mechanisms different from those employed by currently approved antibiotics. In this study, we used several 5-fluorouracil (5-FU) analogues as chemical probes and investigated the potential of these pyrimidine analogues as antibacterial agents. Several 5-FU derivatives exerted potent activity against strains of Gram-positive cocci (GPC) that are susceptible or resistant toward approved antibiotics, without showing cross-resistance. Furthermore, we have provided evidence that the pyrimidine analogues exerted anti-GPC activity via thymineless death by inhibition of thymidylate synthetase (ThyA) and/or inhibition of RNA synthesis. Interestingly, whole genome resequencing of in vitro-selected, pyrimidine analogue-resistant Staphylococcus aureus mutants indicated that S. aureus strains with pyrimidine-analogue resistance induced an amino acid (AA) substitution, deletion, and/or insertion into thymineless-death related proteins except for ThyA, or enhanced the ThyA transcription level. Thus, S. aureus may avoid altering the ThyA function by introducing an AA substitution, suggesting that the pyrimidine analogues, which directly bind to ThyA without phosphorylation, may be more effective and show a higher genetic barrier than the pyrimidines that depend on phosphorylation for activity. The findings of this study may assist in the future development of a novel class of antibiotics for combating MDR GPC, including methicillin-resistant S. aureus and vancomycin-resistant Enterococci. - WQ-3810 inhibits DNA gyrase activity in ofloxacin-resistant Mycobacterium leprae.
Jong-Hoon Park; Tomoyuki Yamaguchi; Yuki Ouchi; Kentaro Koide; Shigetarou Mori; Hyun Kim; Tetsu Mukai; Chie Nakajima; Yasuhiko Suzuki
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 26, 4, 335, 342, 2020年04月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Mycobacterium leprae causes leprosy and ofloxacin is used to control this bacterium. However, specific amino acid substitutions in DNA gyrases of M. leprae interferes with the effect of ofloxacin. METHODOLOGY/PRINCIPAL FINDINGS: Here we tested the inhibitory effect of WQ-3810 on DNA gyrases in M. leprae, using recombinant gyrases. We theorized that WQ-3810 and DNA gyrases interacted, which was tested in silico. Compared with control drugs like ofloxacin, WQ-3810 showed a better inhibitory effect on ofloxacin-resistant DNA gyrases. The in-silico study showed that, unlike control drugs, a specific linkage between a R1 group in WQ-3810 and aspartic acid at position 464 in the subunit B of DNA gyrases existed, which would enhance the inhibitory effect of WQ-3810. This linkage was confirmed in a further experiment, using recombinant DNA gyrases with amino acid substitutions in subunits B instead. CONCLUSIONS/SIGNIFICANCE: The inhibitory effect of WQ-3810 was likely enhanced by the specific linkage between a R1 group residue in its structure and DNA gyrases. Using interactions like the one found in the present work may help design new fluoroquinolones that contribute to halt the emergence of antibiotic-resistant pathogens. - Contribution of β-lactamase and efflux pump overproduction to tazobactam-piperacillin resistance in clinical isolates of Escherichia coli.
Yuuki Suzuki; Toyotaka Sato; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Satoshi Takahashi; Shin-Ichi Yokota
International journal of antimicrobial agents, 55, 4, 105919, 105919, 2020年02月13日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, INTRODUCTION: Tazobactam-piperacillin (TZP) is a mixture of a broad-spectrum penicillin and an irreversible β-lactamase inhibitor. TZP is effective against Gram-negative bacteria that produce extended-spectrum β-lactamases, and it is used as a first-line or second-line drug to treat serious infections. METHODS: This study identified three TZP-resistant and two TZP-intermediate strains among 514 clinical isolates of Escherichia coli. RESULTS: These five isolates possessed one or more β-lactamase genes, blaTEM-1, blaCTX-M-2, blaCTX-M-14, and/or blaCMY-8. The expression levels of β-lactamase genes and acrAB genes in the strains were examined by using real-time reverse transcription PCR. The total enzymatic piperacillin-degrading activity in cells was determined. Two TZP-resistance mechanisms were identified: hyperproduction of TEM-1 in the two resistant strains; and simultaneous high production of β-lactamase and efflux pump AcrAB in the two TZP-intermediate isolates. The latter are an international high-risk clone O25b:H4-ST131-H30R. CONCLUSION: TZP resistance is still rare in clinical isolates of E. coli. However, resistance can develop on high production and/or combinations of known antimicrobial resistance mechanisms in different ways. - In Vitro Derivation of Fluoroquinolone-Resistant Mutants from Multiple Lineages of Haemophilus influenzae and Identification of Mutations Associated with Fluoroquinolone Resistance.
Hiroyuki Honda; Toyotaka Sato; Masaaki Shinagawa; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Koji Kuronuma; Satoshi Takahashi; Hiroki Takahashi; Shin-Ichi Yokota
Antimicrobial agents and chemotherapy, 64, 2, 2020年01月27日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Haemophilus influenzae is a pathogenic bacterium that causes respiratory and otolaryngological infections. The increasing prevalence of β-lactamase-negative high-level ampicillin-resistant H. influenzae (high-BLNAR) is a clinical concern. Fluoroquinolones are alternative agents to β-lactams. However, the emergence and increasing prevalence of fluoroquinolone-resistant H. influenzae have been reported. The current risk of fluoroquinolone resistance in H. influenzae (especially in high-BLNAR) has not yet been evaluated. Here, we examined the development of fluoroquinolone resistance in fluoroquinolone-susceptible clinical H. influenzae isolates in vitro during passaging in the presence of moxifloxacin (from 0.03 to 128 mg/liter). Twenty-nine isolates were examined. Seventeen isolates (58.6%) showed reduced moxifloxacin susceptibility, and 10 of these 17 isolates (34.5% of all isolates) exceeded the Clinical and Laboratory Standards Institute breakpoint for moxifloxacin (MIC of >1 mg/liter) after repeat cultivation on moxifloxacin-containing agar. Seven of these ten isolates were high-BLNAR and represented multiple lineages. We identified 56 novel mutations in 45 genes induced during the development of fluoroquinolone resistance, except the defined quinolone resistance-determining regions (Ser84Leu and Asp88Tyr/Gly/Asn in GyrA and Gly82Asp, Ser84Arg, and Glu88Lys in ParC). Glu153Leu and ΔGlu606 in GyrA, Ser467Tyr and Glu469Asp in GyrB, and ompP2 mutations were novel mutations contributing to fluoroquinolone resistance in H. influenzae In conclusion, H. influenzae clinical isolates from multiple lineages can acquire fluoroquinolone resistance by multiple novel mutations. The higher rate of derivation of fluoroquinolone-resistant H. influenzae from high-BLNAR than β-lactamase-negative ampicillin-susceptible isolates (P = 0.01) raises the possibility of the emergence and spread of fluoroquinolone-resistant high-BLNAR in the clinical setting. - The performance of an in-house loop-mediated isothermal amplification for the rapid detection of Mycobacterium tuberculosis in sputum samples in comparison with Xpert MTB/RIF, microscopy and culture.
Benjawan Phetsuksiri; Wiphat Klayut; Janisara Rudeeaneksin; Sopa Srisungngam; Supranee Bunchoo; Sarawut Toonkomdang; Thanee Wongchai; Chie Nakajima; Yasuhiko Suzuki
Revista do Instituto de Medicina Tropical de Sao Paulo, 62, e36, 2020年, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Simple, low-cost and effective diagnostic tests for tuberculosis (TB) are needed especially in TB-high burden settings. The present study evaluated the performance of an in-house loop-mediated isothermal amplification (LAMP) for diagnosing TB by comparing it to Xpert MTB/RIF, microscopy and culture. In Thailand, a total of 204 excess sputum samples volume after the processing of cultures were used for Mycobacterium tuberculosis (MTB) detection by Xpert MTB/RIF and LAMP. Based on culture results as the gold standard, the overall sensitivity of LAMP and Xpert MTB/RIF were 82.1% (126/153; 95% confidential interval [CI]: 75.4-88.98%) and 86.9 % (133/153; 95% CI: 80.5-90.8%) respectively, and the specificity of both tests was 100% (51/51; 95% CI: 93.0-100.0%). In comparison with Xpert MTB/RIF, the sensitivity and specificity of LAMP were 94.7% (126/133; 95% CI: 89.5-97.9%), and 100.0% (73/73; 95% CI: 94.9-100.0%), respectively. The average threshold cycle (Ct) of Xpert MTB/RIF detection for positive and negative LAMP results was statistically different, of 18.4 and 27.0, respectively (p < 0.05). In comparison with the acid-fast staining technique, and analyzing LAMP and Xpert MTB/RIF in smear-negative/culture-positive specimens, there was an increase of the detection rate by 47.7% (21/44) and 54.6% (24/44). The diagnostic sensitivity and specificity of LAMP appeared to be comparable to those of Xpert MTB/RIF. We claim that this LAMP has potential to provide a sensitive diagnostic test for the rapid TB diagnosis. It allowed a fast detection of MTB before the cultures and it could be used in resource-limited laboratory settings. - Properties of biopolymers detection with piezoelectric polymer biosensor in relaxation behavior process
Mariko Takeda; Kouki Yahagi; Takamichi Hirata; Takashi Kuroiwa; Chie Nakajima; Yasuhiko Suzuki; Fumio Munakata
IEEJ Transactions on Sensors and Micromachines, 140, 2, 43, 49, 2020年, ［査読有り］
研究論文（学術雑誌） - WQ-3810: A new fluoroquinolone with a high potential against fluoroquinolone-resistant Mycobacterium tuberculosis.
Yuki Ouchi; Tetsu Mukai; Kentaro Koide; Tomoyuki Yamaguchi; Jong-Hoon Park; Hyun Kim; Kazumasa Yokoyama; Aki Tamaru; Stephen V Gordon; Chie Nakajima; Yasuhiko Suzuki
Tuberculosis (Edinburgh, Scotland), 120, 101891, 101891, 2020年01月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Fluoroquinolone (FQ) resistance in Mycobacterium tuberculosis (Mtb), caused by amino acid substitutions in DNA gyrase, has been increasingly reported worldwide. WQ-3810 is a newly developed FQ that is highly active against FQ-resistant pathogens; however, its activity against Mtb has not been evaluated. Herein we examined the efficacy of WQ-3810 against Mtb through the use of recombinant Mtb DNA gyrases. In addition, in vitro antimycobacterial activity of WQ-3810 was evaluated against recombinant Mtb var. bovis Bacille Calmette-Guérin strains in which gyrase-coding genes were replaced with Mtb variants containing resistance-conferring mutations. WQ-3810 showed a higher inhibitory activity than levofloxacin against most recombinant DNA gyrases with FQ-resistance mutations. Furthermore, WQ-3810 showed inhibition even against a DNA gyrase variant harboring a G88C mutation which is thought to confer the highest resistance against FQs in clinical Mtb isolates. In contrast, the FQ susceptibility test showed that WQ-3810 had relatively weak mycobactericidal activity compared with moxifloxacin. However, the combination of WQ-3810 and ethambutol showed the greatest degree of synergistic activity against recombinant strains. Since FQs and ethambutol have been used in multi-drug therapy for tuberculosis, WQ-3810 might represent a new, potent anti-tuberculosis drug that can be effective even against FQ-resistant Mtb strains. - Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni.
Matthew V X Whelan; Laura Ardill; Kentaro Koide; Chie Nakajima; Yasuhiko Suzuki; Jeremy C Simpson; Tadhg Ó Cróinín
Scientific reports, 9, 1, 18216, 18216, 2019年12月03日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, The World Health Organization has listed C. jejuni as one of 12 microorganisms on a global priority list for antibiotic resistance due to a rapid increase in strains resistant to fluoroquinolone antibiotics. This fluoroquinolone resistance is conferred through a single point mutation in the QRDR region within the gyrA gene known to be involved in DNA supercoiling. We have previously revealed that changes in DNA supercoilikng play a major role in the regulation of virulence in C. jejuni with relaxation of DNA supercoiling associated with increased attachment to and invasion of human epithelial cells. The aim of this study was to investigate whether fluoroquinolone resistant strains of C. jejuni displayed altered supercoiling associated phenotypes. A panel of fluoroquinolone resistant mutants were derived and shown to have a greater ability to form viable biofilms under aerobic conditions, invade epithelial cells and promote virulence in the Galleria mellonella model of infection. We thus report for the first time that fluoroquinolone resistance in C. jejuni is associated with an increase in virulence and the ability to form viable biofilms in oxygen rich environments. These altered phenotypes likely play a critical role in the continued increase in fluoroquinolone resistance observed for this important pathogen. - WQ-3810 exerts high inhibitory effect on quinolone-resistant DNA gyrase of Salmonella Typhimurium.
Koide K; Kongsoi S; Nakajima C; Suzuki Y
Bioscience, biotechnology, and biochemistry, 83, 12, 2249, 2256, 2019年12月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, The inhibitory effect of WQ-3810 on DNA gyrase was assayed to evaluate the potential of WQ-3810 as a candidate drug for the treatment of quinolone resistant Salmonella Typhymurium infection. The inhibitory effect of WQ-3810, ciprofloxacin and nalidixic acid was compared by accessing the drug concentration that halves the enzyme activity (IC50) of purified S. Typhimurium wildtype and mutant DNA gyrase with amino acid substitution at position 83 or/and 87 in subunit A (GyrA) causing quinolone resistance. As a result, WQ-3810 reduced the enzyme activity of both wildtype and mutant DNA gyrase at a lower concentration than ciprofloxacin and nalidixic acid. Remarkably, WQ-3810 showed a higher inhibitory effect on DNA gyrase with amino acid substitutions at position 87 than with that at position 83 in GyrA. This study revealed that WQ-3810 could be an effective therapeutic agent, especially against quinolone resistant Salmonella enterica having amino acid substitution at position 87. - Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage.
Mendis C; Thevanesam V; Kumara A; Wickramasinghe S; Madegedara D; Gamage C; Gordon SV; Suzuki Y; Ratnatunga C; Nakajima C
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 87, 84, 91, 2019年10月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVE: Sri Lanka is a country where the molecular epidemiology of Mycobacterium tuberculosis (MTB) is poorly explored. Therefore, this study was performed to identify circulating lineages/sub-lineages of MTB and their transmission patterns. METHODS: DNA was extracted from 89 isolates of MTB collected during 2012 and 2013 from new pulmonary tuberculosis patients in Kandy, Sri Lanka and analyzed by spoligotyping, large sequence polymorphism (LSP), mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing, and drug resistance-associated gene sequencing. RESULTS: The predominant lineage was lineage 4 (Euro-American, 45.9%), followed by lineage 1 (Indo-Oceanic, 29.4%), lineage 2 (East-Asian, 23.5%), and lineage 3 (Central-Asian, 1.2%). Among 26 spoligotype patterns, eight were undesignated or new types and seven of these belonged to lineage 4. Undesignated lineage 4/SIT124 (n=2/8) and SIT3234 (n=8/8) clustered together based on 24-locus MIRU-VNTR typing. The dominant sub-lineage was Beijing/SIT1 (n=19), with the isoniazid resistance katG G944C mutation (Ser315Thr) detected in two of them. CONCLUSIONS: The population structure of MTB in Kandy, Sri Lanka was different from that in the South Asian region. The clonal expansion of locally evolved lineage 4/SIT3234 and detection of the pre-multidrug resistant Beijing isolates from new tuberculosis patients is alarming and will require continuous monitoring. - Erratum to "Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar" [Int. J. Infect. Dis. 76 (November) (2018) 109-119].
San LL; Aye KS; Nan Aye TO; Shwe MM; Fukushima Y; Gordon SV; Suzuki Y; Nakajima C
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 85, 214, 214, 2019年08月, ［査読有り］, ［国際誌］
英語 - DNA gyrase could be a crucial regulatory factor for growth and survival of Mycobacterium leprae.
Hyun Kim; Yasuo Fukutomi; Chie Nakajima; Youn Uck Kim; Shigetarou Mori; Keigo Shibayama; Noboru Nakata; Yasuhiko Suzuki
Scientific reports, 9, 1, 10815, 10815, 2019年07月25日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Leprosy, an important infectious disease in humans caused by Mycobacterium leprae (Mle), remains endemic in many countries. Notably, the pathogen cannot be cultured in vitro, except in mouse footpads in vivo. The molecular basis of these characteristics and the mechanisms remain unknown. Consequently, analysis of Mle growth and survival is urgently needed to develop novel therapies against leprosy, including rapid, simple, and specific methods to detect infection. Here, we demonstrated the functional role and contribution of Mle-DNA gyrase, which regulates DNA topology, DNA replication, and chromosome segregation to promote bacterial growth and survival, in Mle growth and survival in vitro and in vivo. The optimum temperature for Mle-DNA gyrase activity was 30 °C. When the DNA gyrB-gyrA genes in Mycobacterium smegmatis were replaced with the Mle gyrase genes by allelic exchange, the recombinants could not grow at 37 °C. Moreover, using radiorespirometry analysis for viability of Mle bacilli, we found that Mle growth was more vigorous at 25-30 °C than at 37 °C, but was inhibited above 40 °C. These results propose that DNA gyrase is a crucial factor for Mle growth and survival and its sensitivity to temperature may be exploited in heat-based treatment of leprosy. - Mixed Mycobacterium tuberculosis Lineage Infection in 2 Elephants, Nepal.
Paudel S; Nakajima C; Mikota SK; Gairhe KP; Maharjan B; Subedi S; Poudel A; Sashika M; Shimozuru M; Suzuki Y; Tsubota T
Emerging infectious diseases, 25, 5, 1031, 1032, 2019年05月, ［査読有り］
英語 - Prevalence of 16S rRNA methylases in Gram-negative bacteria derived from companion animals and livestock in Japan.
Usui M; Kajino A; Kon M; Fukuda A; Sato T; Shirakawa T; Kawanishi M; Harada K; Nakajima C; Suzuki Y; Tamura Y
The Journal of veterinary medical science, 81, 6, 874, 878, 2019年05月, ［査読有り］, ［国内誌］
英語, 研究論文（学術雑誌）, The emergence and spread of aminoglycoside-resistant bacteria are a public health concern. The acquisition of the genes encoding 16S rRNA methylases, such as armA, rmtA, and rmtB, confers high-level resistance to aminoglycosides. However, the prevalence has not been well investigated in Japanese veterinary fields. To determine the prevalence of 16S rRNA methylases in animals, we detected 16S rRNA methylases genes in Gram-negative bacteria from animals. Here, we report the isolation of rmtB amd armA from two of the 446 Escherichia coli (0.5%) and one of the 103 Klebsiella spp. isolates (1.0%) from companion animals, respectively. However, none of the isolations were observed from 2445 E. coli isolates derived from livestock in Japan. The prevalence of 16S rRNA methylases in animals, especially in companion animals, should be carefully monitored in Japanese veterinary fields to avoid the spreading of the genes. - Genetic diversity of Mycobacterium tuberculosis Central Asian Strain isolates from Nepal and comparison with neighboring countries.
Shah Y; Poudel A; Maharjan B; Thapa J; Yamaguchi T; Diab HM; Pandey BD; Solo E; Isoda N; Suzuki Y; Nakajima C
Transactions of the Royal Society of Tropical Medicine and Hygiene, 113, 4, 203, 211, 2019年04月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, BACKGROUND: Multidrug-resistant tuberculosis (MDR-TB) is an emerging threat for successful tuberculosis control worldwide. Central Asian Strain (CAS) has been reported as one of the dominant families contributing to MDR-TB in South Asia including Nepal, India and Pakistan. The aim of this study was to better understand the genetic characteristics of MDR-TB CAS family isolates circulating in Nepal and compare the results with neighboring countries. METHODS: A total of 145 MDR-TB CAS family isolates collected in Nepal from 2008 to 2013 were analyzed by spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) analysis. In addition, we compared these data with published data from India and Pakistan to investigate a possible epidemiological link via construction of a minimum spanning tree (MST). RESULTS: Spoligotyping analysis exhibited CAS1_Delhi SIT26 (n=60) as the predominant lineage among the MDR-TB CAS family in all three countries. However, the combined analysis with spoligotyping and MIRU-VNTR further discriminated 60 isolates into 49 different types and 5 clusters. Each cluster was composed of 14 isolates with a clustering rate of 23.3%, suggesting ongoing transmissions. Based on MST data from neighboring countries, we elucidated an evolutionary relationship between the two countries, Nepal and India, which could be explained by their open border. CONCLUSION: This study identified the evolutionary relationships among MDR-TB CAS1_Delhi subfamily isolates from Nepal and those from neighboring countries. - Evaluation of Susceptibilities to Carbapenems and Faropenem Against Cephalosporin-Resistant Neisseria gonorrhoeae Clinical Isolates with penA Mosaic Alleles.
Yoshiki Hiyama; Satoshi Takahashi; Toyotaka Sato; Masaaki Shinagawa; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki; Naoya Masumori; Shin-Ichi Yokota
Microbial drug resistance (Larchmont, N.Y.), 25, 3, 427, 433, 2019年04月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Neisseria gonorrhoeae is a principal pathogen for sexually transmitted infections, especially for male urethritis. Currently, the prevalence of multidrug resistance is increasing. Carbapenems are broad-spectrum antimicrobials that are widely used in the clinical setting, especially for multidrug-resistant Gram-negative bacteria. However, susceptibility to carbapenems has not been well evaluated for cephalosporin-resistant N. gonorrhoeae isolates. In this study, we determined the susceptibility to a series of carbapenems (meropenem, imipenem, doripenem, and biapenem) and faropenem against cephalosporin-resistant (resistant to cefixime, but susceptible to ceftriaxone) and cephalosporin-susceptible N. gonorrhoeae clinical isolates. The gene mutations associated with β-lactam resistance were evaluated. All cephalosporin-resistant N. gonorrhoeae isolates possessed mosaic mutation alleles in penA (NG-STAR penA-10.001, 27.001, or 108.001). They exhibited a low minimum inhibitory concentration (MIC) (≤0.125 mg/L) for meropenem and markedly high MICs (0.5-2 mg/L) for other carbapenems and faropenem. The strongest association was observed between the mosaic alleles in penA and decreased susceptibility to carbapenems and faropenem compared with mutations in mtrR, porB, and ponA. These results suggest that meropenem may serve as an alternative therapeutic agent for cephalosporin-resistant N. gonorrhoeae with a mosaic allele in penA, whereas other carbapenems and faropenem may be ineffective. - Zinc acetate potentiates the action of tosufloxacin Escherichia coli biofilm persisters.
Usui M; Yokoo H; Tamura Y; Nakajima C; Suzuki Y; Ghigo JM; Beloin C
Antimicrobial agents and chemotherapy, 63, 6, 2019年04月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Formation of bacterial biofilms is a major health threat due to their high levels of tolerance to multiple antibiotics and the presence of persisters responsible for infection relapses. We previously showed that a combination of starvation and induction of SOS response in biofilm led to increased levels of persisters and biofilm tolerance to fluoroquinolones. In this study, we hypothesized that inhibition of the SOS response may be an effective strategy to target biofilms and fluoroquinolone persister cells. We tested the survival of Escherichia coli biofilms to different classes of antibiotics in starved and nonstarved conditions and in the presence of zinc acetate, a SOS response inhibitor. We showed that zinc acetate potentiates, albeit moderately, the activity of fluoroquinolones against E. coli persisters in starved biofilms. The efficacy of zinc acetate to increase fluoroquinolone activity, particularly that of tosufloxacin, suggests that such a combination may be a potential strategy for treating biofilm-related bacterial infections. - 【結核・非結核性抗酸菌症-エキスパートが教える 実臨床に役立つ最新知見】結核・非結核性抗酸菌症の基礎研究 結核菌の薬剤耐性獲得
山口 智之; 中島 千絵; 鈴木 定彦
呼吸器ジャーナル, 66, 4, 650, 656, (株)医学書院, 2018年11月
日本語 - Genetic diversity and distribution dynamics of multidrug-resistant Mycobacterium tuberculosis isolates in Nepal.
Maharjan B; Nakajima C; Isoda N; Thapa J; Poudel A; Shah Y; Yamaguchi T; Shrestha B; Hoffmann H; Avsar K; Shrestha A; Gordon SV; Suzuki Y
Scientific reports, 8, 1, 16634, 16634, 2018年11月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Multidrug-resistant tuberculosis (MDR-TB) is an emerging public health problem in Nepal. Despite the implementation of a successful TB control program in Nepal, notifications of MDR-TB are increasing, yet the reasons are unknown. The objective of this study was to understand the genetic diversity and epidemiological characteristics of MDR-Mycobacterium tuberculosis (MTB) isolates in Nepal. We isolated and genotyped 498 MDR-MTB isolates collected from April 2009 to March 2013 and analyzed the patients' background information. Our results showed that the lineage 2 (Beijing family) was the most predominant lineage (n = 241; 48.4%), followed by lineage 3 (n = 153, 30.7%). Lineage 4 was the third most prevalent (n = 73, 14.5%) followed by lineage 1 (n = 32, 6.4%). The lineages were significantly associated with geographic region, ethnic group, age and sex of patients. The Beijing genotype was found to have an important role in transmitting MDR-TB in Nepal and was significantly associated with the eastern region, mongoloid ethnic group and younger age group. We conclude that early diagnosis and treatment including molecular-epidemiological surveillance of MDR-TB cases will help to control transmission of MDR-TB in Nepal. - Loop-mediated isothermal amplification for rapid identification of Mycobacterium tuberculosis in comparison with immunochromatographic SD Bioline MPT64 Rapid® in a high burden setting.
Phetsuksiri B; Rudeeaneksin J; Srisungngam S; Bunchoo S; Klayut W; Sangkitporn S; Nakajima C; Hamada S; Suzuki Y
Japanese journal of infectious diseases, 72, 2, 112, 114, 2018年10月, ［査読有り］
研究論文（学術雑誌） - Multiclonal Expansion and High Prevalence of β-Lactamase-Negative Haemophilus influenzae with High-Level Ampicillin Resistance in Japan and Susceptibility to Quinolones.
Honda H; Sato T; Shinagawa M; Fukushima Y; Nakajima C; Suzuki Y; Shiraishi T; Kuronuma K; Takahashi S; Takahashi H; Yokota SI
Antimicrobial agents and chemotherapy, 62, 9, 2018年09月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, β-Lactam-resistant Haemophilus influenzae is a clinical concern. A high prevalence (>40%) of β-lactamase-negative high-level ampicillin-resistant H. influenzae (high-BLNAR) isolates in Japan has been reported. However, the reasons for the expansion are unknown. High-BLNAR strains possess an amino acid substitution, either Asn526Lys (group III) or Arg517His (group III-like) in addition to Ser385Thr, in penicillin-binding protein 3 (PBP3). To determine the current prevalence of high-BLNAR strains and the mechanisms behind their expansion in Japan, their prevalence, PBP3 types, multilocus sequence types, and susceptibilities to quinolones approved in Japan as alternatives were determined. Sixty percent of H. influenzae clinical isolates (62/104 isolates) were β-lactamase-negative ampicillin-resistant H. influenzae (BLNAR) strains. Among BLNAR isolates, 92% (57/62 isolates) were high-BLNAR strains. Most isolates were classified as belonging to group III, which contained many genotypes (11 PBP3 types and 25 sequence types). These results indicated that the expansion of high-BLNAR isolates was multiclonal and such strains are still predominant in Japanese clinical settings. One high-BLNAR isolate harbored the novel amino acid substitution Asn526Met in addition to Ser385Thr in PBP3, suggesting a new group (group IV). No quinolone-resistant H. influenzae isolates were identified. The MICs for the quinolones (moxifloxacin, garenoxacin, and tosufloxacin) were similar to that for levofloxacin, whereas sitafloxacin exhibited a lower MIC. However, we obtained 4 H. influenzae isolates with decreased quinolone susceptibility with the amino acid substitution Ser84Leu in GyrA, and 3 of those isolates were high-BLNAR isolates. In summary, this study shows that multiclonal high-BLNAR strains predominate in a Japanese university hospital. Isolates remain sensitive to quinolones, but vigilance is required to prevent the development of fluoroquinolone resistance in high-BLNAR strains. - Whole genome analysis of a multidrug-resistant Streptococcus pneumoniae isolate from a patient with invasive pneumococcal infection developing disseminated intravascular coagulation.
Yasuo Ohkoshi; Toyotaka Sato; Takayuki Wada; Yukari Fukushima; Hiromi Murabayashi; Yasunari Takakuwa; Kaoru Nishiyama; Hiroyuki Honda; Tsukasa Shiraishi; Koji Kuronuma; Hiroki Takahashi; Chie Nakajima; Yasuhiko Suzuki; Shin-Ichi Yokota
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 24, 8, 674, 681, 2018年08月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Multidrug-resistant Streptococcus pneumoniae strains were isolated from blood and sputum of a patient with disseminated intravascular coagulation in Sapporo city, Japan. These antibiograms were only susceptible to vancomycin, linezolid, daptomycin, some carbapenems, and some fluoroquinolones. Identical antibiograms, serotypes (19F), and sequence types (ST10017) suggested a shared origin of these isolates. Only one ST10017 strain has been isolated in the same city in Japan previously (2014), and the 2014 isolate is still susceptible to macrolides. The whole genome of the blood-derived isolate was sequenced. The strain harbored resistance mutations in parC, gyrA, pbp1a, pbp2a, pbp2b, and pbp2x, and harbored the resistance genes, ermB and tetM. The nucleotide sequences of parC and pbp2x genes of strain MDRSPN001 were clearly different from those of other S. pneumoniae strains and were similar to those of oral streptococci strains. These findings suggest that strain MDRSPN001 has been rapidly and drastically evolving multidrug resistance by gene replacement and accumulation of genes originating from other strains, such as oral streptococci, Streptococcus mitis. - Phylogenetic uniqueness of Mycobacterium avium subspecies hominissuis isolated from an abnormal pulmonary bovine case.
Shiomi Yoshida; Tsubasa Araki; Tomohito Asai; Kazunari Tsuyuguchi; Kentaro Arikawa; Tomotada Iwamoto; Chie Nakajima; Yasuhiko Suzuki; Kenji Ohya; Tokuma Yanai; Takayuki Wada; Taro Yamamoto
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 62, 122, 129, 2018年08月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Mycobacterium avium subspecies hominissuis (MAH) is an important cause of infection in human pulmonary and swine intestinal cases. Although MAH is isolated from environmental sources frequently, infections of other animals have rarely been analysed. Recently, we detected granulomatous inflammation in bovine lung as an abnormal postmortem inspection case. To ascertain its genetic profile, we conducted a variable numbers of tandem repeats (VNTR) analysis and genomic characterization using deep sequencing. The VNTR type was a unique profile that differed from reported genotypes, but it was assigned within a broad genotypic complex of isolates from human patients and bathrooms. Genomic comparison with 116 registered genome sequences of the subspecies revealed that the strain was separate from five major genetic population groups proposed previously. Although the infection source remains unclear, its isolation from various resources such as animal infection cases should be elucidated more extensively to reveal its genetic diversity and ecological context. - Characterization of mutations conferring streptomycin resistance to multidrug-resistant Mycobacterium tuberculosis isolates from Myanmar
Nan Aye Thida Oo; Lai Lai San; Jeewan Thapa; Khin Saw Aye; Wah Wah Aung; Chie Nakajima; Yasuhiko Suzuki
Tuberculosis, 111, 8, 13, Churchill Livingstone, 2018年07月01日, ［査読有り］
英語, 研究論文（学術雑誌） - Antibacterial Activity of DC-159a Against Salmonella Typhimurium.
Koide K; Kongsoi S; Ouchi Y; Yamaguchi T; Nakajima C; Suzuki Y
Microbial drug resistance (Larchmont, N.Y.), 25, 1, 14, 22, 2018年07月, ［査読有り］
英語, 研究論文（学術雑誌） - Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar.
San LL; Aye KS; Oo NAT; Shwe MM; Fukushima Y; Gordon SV; Suzuki Y; Nakajima C
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 76, 109, 119, 2018年06月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, OBJECTIVES: Myanmar is a World Health Organization high tuberculosis (TB) burden country with a high multidrug-resistant (MDR)-TB burden. Of significance, a high prevalence of the Beijing genotype of Mycobacterium tuberculosis (MTB) among MDR-MTB has been reported previously. A detailed genetic characterization of TB clinical isolates was performed in order to explore whether there is an association between the prevalence of the Beijing MTB genotype and MDR-TB in Myanmar. METHODS: A total of 265 MDR-MTB clinical isolates collected in 2010 and 2012 were subjected to spoligotyping, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) analysis, single nucleotide polymorphism (SNP) typing, and drug resistance-associated gene sequencing, including rpoC to detect potential compensatory evolution. RESULTS: Of the total MDR-MTB isolates, 79.2% (210/265) were of the Beijing genotype, the majority of which were the 'modern' subtype. Beijing genotype isolates were differentiated by 15-locus MIRU-VNTR and a high clustering rate (53.0%) was observed in the modern subtype. These MIRU-VNTR patterns were similar to Beijing genotype clones spreading across Russia and Central Asia. A high prevalence of katG Ser315Thr, and genetic evidence of extensive drug resistance (XDR) and pre-XDR and compensatory mutations in rpoC were observed among clustered isolates. CONCLUSIONS: MDR-MTB strains of the Beijing genotype might be spreading in Myanmar and present a major challenge to TB control in this country. - Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease.
Yamato Sajiki; Satoru Konnai; Tomohiro Okagawa; Asami Nishimori; Naoya Maekawa; Shinya Goto; Ryoyo Ikebuchi; Reiko Nagata; Satoko Kawaji; Yumiko Kagawa; Shinji Yamada; Yukinari Kato; Chie Nakajima; Yasuhiko Suzuki; Shiro Murata; Yasuyuki Mori; Kazuhiko Ohashi
Infection and immunity, 86, 5, 2018年05月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis, is a bovine chronic infection that is endemic in Japan and many other countries. The expression of immunoinhibitory molecules is upregulated in cattle with Johne's disease, but the mechanism of immunosuppression is poorly understood. Prostaglandin E2 (PGE2) is immunosuppressive in humans, but few veterinary data are available. In this study, functional and kinetic analyses of PGE2 were performed to investigate the immunosuppressive effect of PGE2 during Johne's disease. In vitro PGE2 treatment decreased T-cell proliferation and Th1 cytokine production and upregulated the expression of immunoinhibitory molecules such as interleukin-10 and programmed death ligand 1 (PD-L1) in peripheral blood mononuclear cells (PBMCs) from healthy cattle. PGE2 was upregulated in sera and intestinal lesions of cattle with Johne's disease. In vitro stimulation with Johnin purified protein derivative (J-PPD) induced cyclooxygenase-2 (COX-2) transcription, PGE2 production, and upregulation of PD-L1 and immunoinhibitory receptors in PBMCs from cattle infected with M. avium subsp. paratuberculosis Therefore, Johnin-specific Th1 responses could be limited by the PGE2 pathway in cattle. In contrast, downregulation of PGE2 with a COX-2 inhibitor promoted J-PPD-stimulated CD8+ T-cell proliferation and Th1 cytokine production in PBMCs from the experimentally infected cattle. PD-L1 blockade induced J-PPD-stimulated CD8+ T-cell proliferation and interferon gamma production in vitro Combined treatment with a COX-2 inhibitor and anti-PD-L1 antibodies enhanced J-PPD-stimulated CD8+ T-cell proliferation in vitro, suggesting that the blockade of both pathways is a potential therapeutic strategy to control Johne's disease. The effects of COX-2 inhibition warrant further study as a novel treatment of Johne's disease. - Genotypic characterization of pyrazinamide resistance in Mycobacterium tuberculosis isolated from Lusaka, Zambia
Precious Bwalya; Tomoyuki Yamaguchi; Georgina Mulundu; Chie Nakajima; Grace Mbulo; Eddie Samuneti Solo; Yukari Fukushima; Kunda Kasakwa; Yasuhiko Suzuki
Tuberculosis, 109, 117, 122, Churchill Livingstone, 2018年03月01日, ［査読有り］
英語, 研究論文（学術雑誌） - Tuberculosis Caused by Mycobacterium orygis in Dairy Cattle and Captured Monkeys in Bangladesh: a New Scenario of Tuberculosis in South Asia
Z. Rahim; J. Thapa; Y. Fukushima; A. G. M. van der Zanden; S. V. Gordon; Y. Suzuki; C. Nakajima
TRANSBOUNDARY AND EMERGING DISEASES, 64, 6, 1965, 1969, 2017年12月, ［査読有り］
英語, 研究論文（学術雑誌） - Complete Genome Sequence of Multidrug-Resistant Streptococcus pneumoniae Serotype 19F Isolated from an Invasive Infection in Sapporo, Japan.
Toyotaka Sato; Yasuo Ohkoshi; Takayuki Wada; Yukari Fukushima; Hiromi Murabayashi; Yasunari Takakuwa; Kaoru Nishiyama; Tsukasa Shiraishi; Chie Nakajima; Yasuhiko Suzuki; Shin-Ichi Yokota
Genome announcements, 5, 44, 2017年11月02日, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌）, Invasive infection of multidrug-resistant Streptococcus pneumoniae is a serious clinical concern. Here, we report the complete genome sequence of a multidrug-resistant S. pneumoniae serotype 19F strain isolated from a patient with an invasive infection in Sapporo, Japan. - Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
Fuangfa Utrarachkij; Chie Nakajima; Ruchirada Changkwanyeun; Kanokrat Siripanichgon; Siriporn Kongsoi; Srirat Pornruangwong; Kanjana Changkaew; Risa Tsunoda; Yutaka Tamura; Orasa Suthienkul; Yasuhiko Suzuki
MICROBIAL DRUG RESISTANCE, 23, 7, 885, 894, 2017年10月, ［査読有り］
英語, 研究論文（学術雑誌） - High diversity of multidrug-resistant Mycobacterium tuberculosis Central Asian Strain isolates in Nepal
Yogendra Shah; Bhagwan Maharjan; Jeewan Thapa; Ajay Poudel; Hassan Mahmoud Diab; Basu Dev Pandey; Eddie S. Solo; Norikazu Isoda; Yasuhiko Suzuki; Chie Nakajima
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 63, 13, 20, 2017年10月, ［査読有り］
英語, 研究論文（学術雑誌） - ANTIMICROBIAL RESISTANCE, INTEGRON, VIRULENCE GENE, AND MULTILOCUS SEQUENCE TYPING OF &ITESCHERICHIA COLI&IT ISOLATES FROM POSTWEANING PIGLETS WITH AND WITHOUT DIARRHEA
Watsawan Prapasawat; Apiradee Intarapuk; Pornthip Chompook; Chie Nakajima; Yasuhiko Suzuki; Orasa Suthienkul
SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH, 48, 5, 1042, 1055, 2017年09月, ［査読有り］
英語, 研究論文（学術雑誌） - Population Structure and Local Adaptation of MAC Lung Disease Agent Mycobacterium avium subsp hominissuis
Hirokazu Yano; Tomotada Lwamoto; Yukiko Nishiuchi; Chie Nakajima; Daria A. Starkova; Igor Mokrousov; Olga Narvskaya; Shiomi Yoshida; Kentaro Arikawa; Noriko Nakanishi; Ken Osaki; Ichiro Nakagawa; Manabu Ato; Yasuhiko Suzuki; Fumito Maruyama
GENOME BIOLOGY AND EVOLUTION, 9, 9, 2403, 2417, 2017年09月, ［査読有り］
英語, 研究論文（学術雑誌） - A canine chimeric monoclonal antibody targeting PD-L1 and its clinical efficacy in canine oral malignant melanoma or undifferentiated sarcoma
Naoya Maekawa; Satoru Konnai; Satoshi Takagi; Yumiko Kagawa; Tomohiro Okagawa; Asami Nishimori; Ryoyo Ikebuchi; Yusuke Izumi; Tatsuya Deguchi; Chie Nakajima; Yukinari Kato; Keiichi Yamamoto; Hidetoshi Uemura; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
SCIENTIFIC REPORTS, 7, 1, 8951, 2017年08月, ［査読有り］
英語, 研究論文（学術雑誌） - A quantitative and efficient approach to select MIRU-VNTR loci based on accumulation of the percentage differences of strains for discriminating divergent Mycobacterium tuberculosis sublineages
Xin-Ling Pan; Chun-Lei Zhang; Chie Nakajima; Jin Fu; Chang-Xia Shao; Li-Na Zhao; Jia-Yi Cui; Na Jiao; Chang-Long Fan; Yasuhiko Suzuki; Toshio Hattori; Di Li; Hong Ling
EMERGING MICROBES & INFECTIONS, 6, 7, e68, 2017年07月, ［査読有り］
英語, 研究論文（学術雑誌） - Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle
Tomohiro Okagawa; Satoru Konnai; Asami Nishimori; Naoya Maekawa; Ryoyo Ikebuchi; Shinya Goto; Chie Nakajima; Junko Kohara; Satoshi Ogasawara; Yukinari Kato; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
FRONTIERS IN IMMUNOLOGY, 8, 650, 2017年06月, ［査読有り］
英語, 研究論文（学術雑誌） - Evaluation of in-house loop-mediated isothermal amplification for tuberculosis diagnosis compared with Xpert MTB/RIF
Charity Habeenzu; Chie Nakajima; Eddie Solo; Precious Bwalya; Kiichi Kajino; Mari Miller; Youichi Kurosawa; Victor Mudenda; Lackson Kasonka; Yasuhiko Suzuki; Takashi Matsuba
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, 11, 6, 440, 444, 2017年06月, ［査読有り］
英語, 研究論文（学術雑誌） - Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study
Jingge Zhao; Takashi Matsuba; Xiaoyan Zhang; Susan Leano; Chie Nakajima; Haorile Chagan-Yasutan; Elizabeth Freda Telan; Yasuhiko Suzuki; Toshio Hattori
BMC INFECTIOUS DISEASES, 17, 1, 344, 2017年05月, ［査読有り］
英語, 研究論文（学術雑誌） - Survival and prevalence of Clostridium difficile in manure compost derived from pigs
Masaru Usui; Mayuko Kawakura; Nobuki Yoshizawa; Lai Lai San; Chie Nakajima; Yasuhiko Suzuki; Yutaka Tamura
ANAEROBE, 43, 15, 20, 2017年02月, ［査読有り］
英語, 研究論文（学術雑誌） - Immunological Roles of Elevated Plasma Levels of Matricellular Proteins in Japanese Patients with Pulmonary Tuberculosis
Beata Shiratori; Jingge Zhao; Masao Okumura; Haorile Chagan-Yasutan; Hideki Yanai; Kazue Mizuno; Takashi Yoshiyama; Tadashi Idei; Yugo Ashino; Chie Nakajima; Yasuhiko Suzuki; Toshio Hattori
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18, 1, 2017年01月, ［査読有り］
英語, 研究論文（学術雑誌） - Difference in Antibody Responses to Mycobacterium tuberculosis Antigens in Japanese Tuberculosis Patients Infected with the Beijing/Non-Beijing Genotype
Jingge Zhao; Beata Shiratori; Masao Okumura; Hideki Yanai; Makoto Matsumoto; Chie Nakajima; Kazue Mizuno; Kenji Ono; Tetsuya Oda; Haorile Chagan-Yasutan; Yugo Ashino; Takashi Matsuba; Takashi Yoshiyama; Yasuhiko Suzuki; Toshio Hattori
JOURNAL OF IMMUNOLOGY RESEARCH, 2017, 4797856, 2017年, ［査読有り］
英語, 研究論文（学術雑誌） - Quinolone resistance-associated amino acid substitutions affect enzymatic activity of Mycobacterium leprae DNA gyrase
Tomoyuki Yamaguchi; Kazumasa Yokoyama; Chie Nakajima; Yasuhiko Suzuki
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81, 7, 1343, 1347, 2017年, ［査読有り］
英語, 研究論文（学術雑誌） - Genotypic diversity of Mycobacterium tuberculosis strains in Myanmar
Thanda Tun; Khin Saw Aye; Wint Wint Nyunt; John A. Crump; Chie Nakajima; Yasuhiko Suzuki; Kyi Kyi Thinn; Gregory M. Cook; Htin Lin Aung
INFECTIOUS DISEASES, 49, 3, 237, 239, 2017年, ［査読有り］
英語 - Molecular epidemiology of pathogenic Leptospira spp. among large ruminants in the Philippines
Marvin A. Villanueva; Claro N. Mingala; Michelle M. Balbin; Chie Nakajima; Norikazu Isoda; Yasuhiko Suzuki; Nobuo Koizumi
JOURNAL OF VETERINARY MEDICAL SCIENCE, 78, 11, 1649, 1655, 2016年11月, ［査読有り］
英語, 研究論文（学術雑誌） - Impact of mutations in DNA gyrase genes on quinolone resistance in Campylobacter jejuni
Ruchirada Changkwanyeun; Tomoyuki Yamaguchi; Siriporn Kongsoi; Kanjana Changkaew; Kazumasa Yokoyama; Hyun Kim; Orasa Suthienkul; Masaru Usui; Yutaka Tamura; Chie Nakajima; Yasuhiko Suzuki
DRUG TESTING AND ANALYSIS, 8, 10, 1071, 1076, 2016年10月, ［査読有り］
英語, 研究論文（学術雑誌） - Amino acid substitutions in GyrA affect quinolone susceptibility in Salmonella typhimurium
Siriporn Kongsoi; Ruchirada Changkwanyeun; Kazumasa Yokoyama; Chie Nakajima; Kanjana Changkaew; Orasa Suthienkul; Yasuhiko Suzuki
DRUG TESTING AND ANALYSIS, 8, 10, 1065, 1070, 2016年10月, ［査読有り］
英語, 研究論文（学術雑誌） - DC-159a Shows Inhibitory Activity against DNA Gyrases of Mycobacterium leprae
Tomoyuki Yamaguchi; Kazumasa Yokoyama; Chie Nakajima; Yasuhiko Suzuki
PLoS Neglected Tropical Diseases, 10, 9, e0005013, Public Library of Science, 2016年09月28日, ［査読有り］
英語, 研究論文（学術雑誌） - DC-159a Shows Inhibitory Activity against DNA Gyrases of Mycobacterium leprae
Tomoyuki Yamaguchi; Kazumasa Yokoyama; Chie Nakajima; Yasuhiko Suzuki
PLOS NEGLECTED TROPICAL DISEASES, 10, 9, 2016年09月, ［査読有り］
英語, 研究論文（学術雑誌） - The effects of administering lactic acid bacteria sealed in a capsule on the intestinal bacterial flora of cattle
Tomohiro Seyama; Hirofumi Hirayasu; Gen Yoshida; Aiko Ohnuma; Yongjin Qiu; Chie Nakajima; Koji Kasai; Yasuhiko Suzuki
JAPANESE JOURNAL OF VETERINARY RESEARCH, 64, 3, 197, 203, 2016年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus).
Paudel S; Villanueva MA; Mikota SK; Nakajima C; Gairhe KP; Subedi S; Rayamajhi N; Sashika M; Shimozuru M; Matsuba T; Suzuki Y; Tsubota T
The Journal of veterinary medical science / the Japanese Society of Veterinary Science, 78, 7, 1117, 1121, 2016年07月, ［査読有り］
英語, 研究論文（学術雑誌） - Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma
Naoya Maekawa; Satoru Konnai; Tomohiro Okagawa; Asami Nishimori; Ryoyo Ikebuchi; Yusuke Izumi; Satoshi Takagi; Yumiko Kagawa; Chie Nakajima; Yasuhiko Suzuki; Yukinari Kato; Shiro Murata; Kazuhiko Ohashi
PLOS ONE, 11, 6, e0157176, 2016年06月, ［査読有り］
英語, 研究論文（学術雑誌） - Antigenic characterization of dimorphic surface protein in Mycobacterium tuberculosis
Takashi Matsuba; Umme Ruman Siddiqi; Toshio Hattori; Chie Nakajima; Jun Fujii; Yasuhiko Suzuki
FEMS MICROBIOLOGY LETTERS, 363, 10, 2016年05月, ［査読有り］
英語, 研究論文（学術雑誌） - Development of Point-of-Care Testing for Disaster-Related Infectious Diseases
Toshio Hattori; Haorile Chagan-Yasutan; Beata Shiratori; Shinichi Egawa; Takako Izumi; Toru Kubo; Chie Nakajima; Yasuhiko Suzuki; Toshiro Niki; Bachti Alisjahbana; Elizabeth Telan
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 238, 4, 287, 293, 2016年04月, ［査読有り］
英語 - Genetic diversity and antimicrobial resistance pattern of Salmonella enterica serovar Enteritidis clinical isolates in Thailand
Fuangfa Utrarachkij; Chie Nakajima; Kanokrat Siripanichgon; Kanjana Changkaew; Yuwanda Thongpanich; Srirat Pornraungwong; Orasa Suthienkul; Yasuhiko Suzuki
JOURNAL OF INFECTION AND CHEMOTHERAPY, 22, 4, 209, 215, 2016年04月, ［査読有り］
英語, 研究論文（学術雑誌） - Combined antibody and DNA detection for early diagnosis of leptospirosis after a disaster
Hiroko Iwasaki; Haorile Chagan-Yasutan; Prisca Susan A. Leano; Nobuo Koizumi; Chie Nakajima; Delsi Taurustiati; Firmanto Hanan; Talitha Lea Lacuesta; Yugo Ashino; Yasuhiko Suzuki; Nina G. Gloriani; Elizabeth Freda O. Telan; Toshio Hattori
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 84, 4, 287, 291, 2016年04月, ［査読有り］
英語, 研究論文（学術雑誌） - Mycobacterium orygis-Associated Tuberculosis in Free-Ranging Rhinoceros, Nepal, 2015
Jeewan Thapa; Sarad Paudel; Amir Sadaula; Yogendra Shah; Bhagwan Maharjan; Gretchen E. Kaufman; Deborah McCauley; Kamal P. Gairhe; Toshio Tsubota; Yasuhiko Suzuki; Chie Nakajima
EMERGING INFECTIOUS DISEASES, 22, 3, 570, 572, 2016年03月, ［査読有り］
英語 - Genotypic characterization of multi-drug-resistant Mycobacterium tuberculosis isolates in Myanmar
Khin Saw Aye; Chie Nakajima; Tomoyuki Yamaguchi; Min Min Win; Mu Mu Shwe; Aye Aye Win; Thandar Lwin; Wint Wint Nyunt; Ti Ti; Yasuhiko Suzuki
JOURNAL OF INFECTION AND CHEMOTHERAPY, 22, 3, 174, 179, 2016年03月, ［査読有り］
英語, 研究論文（学術雑誌） - Serological investigation of Leptospira infection and its circulation in one intensive-type water buffalo farm in the Philippines
Marvin A. Villanueva; Claro N. Mingala; Nina G. Gloriani; Yasutake Yanagihara; Norikazu Isoda; Chie Nakajima; Yasuhiko Suzuki; Nobuo Koizumi
JAPANESE JOURNAL OF VETERINARY RESEARCH, 64, 1, 15, 24, 2016年02月, ［査読有り］
英語, 研究論文（学術雑誌） - Detection of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Market-Ready Chickens in Zambia
K. Chishimba; B. M. Hang'Ombe; K. Muzandu; S. E. Mshana; M. I. Matee; C. Nakajima; Y. Suzuki
International Journal of Microbiology, 2016, Hindawi Limited, 2016年, ［査読有り］
英語, 研究論文（学術雑誌） - First insight into the genetic population structure of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in Egypt
Hassan Mahmoud Diab; Chie Nakajima; Saber A. Kotb; Alaa Mokhtar; Nagwa F. M. Khder; Ahmed S. A. Abdelaal; Azza Hegazy; Ajay Poudel; Yogendra Shah; Yasuhiko Suzuki
TUBERCULOSIS, 96, 13, 20, 2016年01月, ［査読有り］
英語, 研究論文（学術雑誌） - Multiple-locus variable-number tandem repeat analysis of Leptospira interrogans and Leptospira borgpetersenii isolated from small feral and wild mammals in East Asia
Nobuo Koizumi; Hidemasa Izumiya; Jung-Jung Mu; Zbigniew Arent; Shou Okano; Chie Nakajima; Yasuhiko Suzuki; Maki Mizutani Muto; Tsutomu Tanikawa; Kyle R. Taylor; Noriyuki Komatsu; Kumiko Yoshimatsu; Hoang Thi Thu Ha; Makoto Ohnishi
INFECTION GENETICS AND EVOLUTION, 36, 434, 440, 2015年12月, ［査読有り］
英語, 研究論文（学術雑誌） - Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan
Ryuji Kawahara; Kazuko Seto; Masumi Taguchi; Chie Nakajima; Yuko Kumeda; Yasuhiko Suzuki
JOURNAL OF CLINICAL MICROBIOLOGY, 53, 9, 3035, 3038, 2015年09月, ［査読有り］
英語, 研究論文（学術雑誌） - Molecular characterization of Mycobacterium orygis isolates from wild animals of Nepal
Jeewan Thapa; Chie Nakajima; Bhagwan Maharjan; Ajay Poudel; Yasuhiko Suzuki
JAPANESE JOURNAL OF VETERINARY RESEARCH, 63, 3, 151, 158, 2015年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Characterization of Campylobacter jejuni DNA gyrase as the target of quinolones
Ruchirada Changkwanyeun; Masaru Usui; Siriporn Kongsoi; Kazumasa Yokoyama; Hyun Kim; Orasa Suthienkul; Kanjana Changkaew; Chie Nakajima; Yutaka Tamura; Yasuhiko Suzuki
JOURNAL OF INFECTION AND CHEMOTHERAPY, 21, 8, 604, 609, 2015年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Antimicrobial Resistance, Extended-Spectrum β-Lactamase Productivity, and Class 1 Integrons in Escherichia coli from Healthy Swine.
Changkaew K; Intarapuk A; Utrarachkij F; Nakajima C; Suthienkul O; Suzuki Y
Journal of food protection, 78, 8, 1442, 1450, 2015年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Characterization of Salmonella Typhimurium DNA gyrase as a target of quinolones
Siriporn Kongsoi; Kazumasa Yokoyama; Apinun Suprasert; Fuangfa Utrarachkij; Chie Nakajima; Orasa Suthienkul; Yasuhiko Suzuki
DRUG TESTING AND ANALYSIS, 7, 8, 714, 720, 2015年08月, ［査読有り］
英語, 研究論文（学術雑誌） - A case of Manila type Mycobacterium tuberculosis infection in Japan.
Usami O; Nakajima C; Endo S; Inomata S; Kanamori H; Hirakata Y; Uchiyama B; Kaku M; Suzuki Y; Hattori T
Clinical case reports, 3, 7, 622, 625, 2015年07月, ［査読有り］, ［国際誌］
英語, A 76-year-old Japanese woman contracted a Mycobacterium tuberculosis (TB, Manila type) infection in Japan, despite never having traveled. However, her son was treated for TB in the Philippines 3 years before he stayed at her house. Spoligotyping allows us to identify the TB genotype and identify the route of infection. - Bacterial Diversity in Sea Ice from the Southern Ocean and the Sea of Okhotsk
Okubo T; Tosaka Y; Sato T; Usui M; Nakajima C; Suzuki Y; Imura S; Tamura Y
Journal of Applied & Environmental Microbiology, 2, 6, 266, 272, 2014年10月, ［査読有り］ - Dominant modern sublineages and a new modern sublineage of Mycobacterium tuberculosis Beijing family clinical isolates in Heilongjiang Province, China
Di Li; Cai-Bo Dong; Jia-Yi Cui; Chie Nakajima; Chun-Lei Zhang; Xin-Ling Pan; Gao-Xiang Sun; En-Yu Dai; Yasuhiko Suzuki; Min Zhuang; Hong Ling
INFECTION GENETICS AND EVOLUTION, 27, 294, 299, 2014年10月, ［査読有り］
英語, 研究論文（学術雑誌） - Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes
Ryoyo Ikebuchi; Satoru Konnai; Tomohiro Okagawa; Kazumasa Yokoyama; Chie Nakajima; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
IMMUNOLOGY, 142, 4, 551, 561, 2014年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Characterization of Antibiotic Resistance in Escherichia coli Isolated from Shrimps and Their Environment
Kanjana Changkaew; Fuangfa Utrarachkij; Kanokrat Siripanichgon; Chie Nakajima; Orasa Suthienkul; Yasuhiko Suzuki
JOURNAL OF FOOD PROTECTION, 77, 8, 1394, 1401, 2014年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Identification and characterization of bovine programmed death-ligand 2
Asami Nishimori; Satoru Konnai; Ryoyo Ikebuchi; Tomohiro Okagawa; Chie Nakajima; Yasuhiko Suzuki; Claro N. Mingala; Shiro Murata; Kazuhiko Ohashi
MICROBIOLOGY AND IMMUNOLOGY, 58, 7, 388, 397, 2014年07月, ［査読有り］
英語, 研究論文（学術雑誌） - Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-γ Production from Tumor-Infiltrating Cells by PD-L1 Blockade.
Maekawa N; Konnai S; Ikebuchi R; Okagawa T; Adachi M; Takagi S; Kagawa Y; Nakajima C; Suzuki Y; Murata S; Ohashi K
PloS one, 9, 6, e98415, 2014年06月, ［査読有り］
英語, 研究論文（学術雑誌） - Molecular characterization of Mycobacterium tuberculosis isolates from elephants of Nepal.
Paudel S; Mikota SK; Nakajima C; Gairhe KP; Maharjan B; Thapa J; Poudel A; Shimozuru M; Suzuki Y; Tsubota T
Tuberculosis (Edinburgh, Scotland), 94, 3, 287, 292, 2014年05月, ［査読有り］
英語, 研究論文（学術雑誌） - Intra-subspecies sequence variability of the MACPPE12 gene in Mycobacterium avium subsp hominissuis
Tomotada Iwamoto; Kentaro Arikawa; Chie Nakajima; Noriko Nakanishi; Yukiko Nishiuchi; Shiomi Yoshida; Aki Tamaru; Yutaka Tamura; Yoshihiko Hoshino; Heekyung Yoo; Young Kil Park; Hajime Saito; Yasuhiko Suzuki
INFECTION GENETICS AND EVOLUTION, 21, 479, 483, 2014年01月, ［査読有り］
英語, 研究論文（学術雑誌） - Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients
Beata Shiratori; Susan Leano; Chie Nakajima; Haorile Chagan-Yasutan; Toshiro Niki; Yugo Ashino; Yasuhiko Suzuki; Elisabeth Telan; Toshio Hattori
MEDIATORS OF INFLAMMATION, 2014, 513263, 2014年, ［査読有り］
英語, 研究論文（学術雑誌） - What hansen’s disease research learned from tuberculosis research: From molecular biological aspect
Yasuhiko Suzuki; Tomoyuki Yamaguchi; Hyun Kim; Kazumasa Yokoyama; Chie Nakajima
Japanese Journal of Leprosy, 83, 3, 131, 137, Japanese Leprosy Association, 2014年, ［査読有り］
日本語, 研究論文（学術雑誌） - Applicability of In-House Loop-Mediated Isothermal Amplification for Rapid Identification of Mycobacterium tuberculosis Complex Grown on Solid Media
Benjawan Phetsuksiri; Janisara Rudeeaneksin; Sopa Srisungngam; Supranee Bunchoo; Dhanida Roienthong; Tetsu Mukai; Chie Nakajima; Shigeyuki Hamada; Yasuhiko Suzuki
JAPANESE JOURNAL OF INFECTIOUS DISEASES, 66, 3, 249, 251, 2013年05月, ［査読有り］
英語, 研究論文（学術雑誌） - Simple multiplex PCR assay for identification of Beijing family Mycobacterium tuberculosis isolates with a lineage-specific mutation in Rv0679c
Chie Nakajima; Aki Tamaru; Zeaur Rahim; Ajay Poudel; Bhagwan Maharjan; Khin Saw Aye; Hong Ling; Toshio Hattori; Tomotada Iwamoto; Yukari Fukushima; Haruka Suzuki; Yasuhiko Suzuki; Takashi Matsuba
Journal of Clinical Microbiology, 51, 7, 2025, 2032, American Society for Microbiology, 2013年, ［査読有り］
英語, 研究論文（学術雑誌） - Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro
Ryoyo Ikebuchi; Satoru Konnai; Tomohiro Okagawa; Kazumasa Yokoyama; Chie Nakajima; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi
Veterinary Research, 44, 1, 59, 1, 2013年, ［査読有り］
英語, 研究論文（学術雑誌） - Characterization of extensively drug-resistant Mycobacterium tuberculosis in Nepal
Ajay Poudel; Bhagwan Maharjan; Chie Nakajima; Yukari Fukushima; Basu D. Pandey; Antje Beneke; Yasuhiko Suzuki
TUBERCULOSIS, 93, 1, 84, 88, 2013年01月, ［査読有り］
英語, 研究論文（学術雑誌） - Molecular mechanism of rifampicin and isoniazid resistance in Mycobacterium tuberculosis from Bangladesh
Zeaur Rahim; Chie Nakajima; Rubhana Raqib; Khalequ Zaman; Hubert P. Endtz; Adri G. M. van der Zanden; Yasuhiko Suzuki
TUBERCULOSIS, 92, 6, 529, 534, 2012年11月, ［査読有り］
英語, 研究論文（学術雑誌） - Mycobacterium bovis infection at the interface between domestic and wild animals in Zambia
Mudenda B. Hang'ombe; Musso Munyeme; Chie Nakajima; Yukari Fukushima; Haruka Suzuki; Wigganson Matandiko; Akihiro Ishii; Aaron S. Mweene; Yasuhiko Suzuki
BMC VETERINARY RESEARCH, 8, 221, 2012年11月, ［査読有り］
英語, 研究論文（学術雑誌） - Impact of Amino Acid Substitutions in B Subunit of DNA Gyrase in Mycobacterium leprae on Fluoroquinolone Resistance
Kazumasa Yokoyama; Hyun Kim; Tetsu Mukai; Masanori Matsuoka; Chie Nakajima; Yasuhiko Suzuki
PLoS Neglected Tropical Diseases, 6, 10, e1838, 10, 2012年10月, ［査読有り］
英語, 研究論文（学術雑誌） - Impact of Amino Acid Substitutions in B Subunit of DNA Gyrase in Mycobacterium leprae on Fluoroquinolone Resistance
Kazumasa Yokoyama; Hyun Kim; Tetsu Mukai; Masanori Matsuoka; Chie Nakajima; Yasuhiko Suzuki
PLOS NEGLECTED TROPICAL DISEASES, 6, 10, 2012年10月, ［査読有り］
英語, 研究論文（学術雑誌） - Pulmonary tuberculosis in patients with diabetes mellitus in Bangladesh
Zeaur Rahim; Mst. Sabiha Banu Momi; Sajal K. Saha; K. Zaman; Khwaja Nazim Uddin; S. N. A. Ashraf Jamil; Nazmun Nahar; A. K. Azad Khan; E. A. W. D. Cooreman; Motiuddin Ahmed; A. G. M. van der Zanden; Chie Nakajima; Yasuhiko Suzuki
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 16, 8, 1132, 1133, 2012年08月, ［査読有り］
英語 - Dominant Incidence of Multidrug and Extensively Drug-Resistant Specific Mycobacterium tuberculosis Clones in Osaka Prefecture, Japan
Aki Tamaru; Chie Nakajima; Takayuki Wada; Yajun Wang; Manabu Inoue; Ryuji Kawahara; Ryoji Maekura; Yuriko Ozeki; Hisashi Ogura; Kazuo Kobayashi; Yasuhiko Suzuki; Sohkichi Matsumoto
PLOS ONE, 7, 8, e42505, 2012年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification
Janisara Rudeeaneksin; Supranee Bunchoo; Sopa Srisungngam; Pathom Sawanpanyalert; Sawet Chamnangrom; Atipa Kamolwat; Porntip Thanasripakdeekul; Tooru Taniguchi; Chie Nakajima; Yasuhiko Suzuki; Benjawan Phetsuksiri
JAPANESE JOURNAL OF INFECTIOUS DISEASES, 65, 4, 306, 311, 2012年07月, ［査読有り］
英語, 研究論文（学術雑誌） - Genetic diversity of Mycobacterium avium subsp hominissuis strains isolated from humans, pigs, and human living environment
Tomotada Iwamoto; Chie Nakajima; Yukiko Nishiuchi; Tomoko Kato; Shiomi Yoshida; Noriko Nakanishi; Aki Tamaru; Yutaka Tamura; Yasuhiko Suzuki; Masao Nasu
INFECTION GENETICS AND EVOLUTION, 12, 4, 846, 852, 2012年06月, ［査読有り］
英語, 研究論文（学術雑誌） - A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine
Nobuo Koizumi; Chie Nakajima; Tsunehito Harunari; Tsutomu Tanikawa; Toshihiro Tokiwa; Eriko Uchimura; Tokujiro Furuya; Claro Niegos Mingala; Marvin Ardeza Villanueva; Makoto Ohnishi; Yasuhiko Suzuki
JOURNAL OF CLINICAL MICROBIOLOGY, 50, 6, 2072, 2074, 2012年06月, ［査読有り］
英語, 研究論文（学術雑誌） - Quinolone-Induced Upregulation of Osteopontin Gene Promoter Activity in Human Lung Epithelial Cell Line A549
Beata Shiratori; Jing Zhang; Osamu Usami; Haorile Chagan-Yasutan; Yasuhiko Suzuki; Chie Nakajima; Toshimitsu Uede; Toshio Hattori
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56, 6, 2868, 2872, 2012年06月, ［査読有り］
英語, 研究論文（学術雑誌） - Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal
Ajay Poudel; Chie Nakajima; Yukari Fukushima; Haruka Suzuki; Basu Dev Pandey; Bhagwan Maharjan; Yasuhiko Suzuki
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56, 6, 2831, 2836, 2012年06月, ［査読有り］
英語, 研究論文（学術雑誌） - Sensitivities of ciprofloxacin-resistant Mycobacterium tuberculosis clinical isolates to fluoroquinolones: role of mutant DNA gyrase subunits in drug resistance
Yasuhiko Suzuki; Chie Nakajima; Aki Tamaru; Hyun Kim; Takashi Matsuba; Hajime Saito
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 39, 5, 435, 439, 2012年05月, ［査読有り］
英語, 研究論文（学術雑誌） - A Rapid Loop-Mediated Isothermal Amplification Assay Targeting hspX for the Detection of Mycobacterium tuberculosis Complex
Aixiao Bi; Chie Nakajima; Yukari Fukushima; Aki Tamaru; Isamu Sugawara; Akio Kimura; Ryuji Kawahara; Zhongyi Hu; Yasuhiko Suzuki
JAPANESE JOURNAL OF INFECTIOUS DISEASES, 65, 3, 247, 251, 2012年05月, ［査読有り］
英語, 研究論文（学術雑誌） - Distinct Clinical Features in Nontuberculous Mycobacterial Disease with or without Latent Tuberculosis Infection
Umme Ruman Siddiqi; Haorile Chagan-Yasutan; Chie Nakajima; Hiroki Saitoh; Yugo Ashino; Osamu Usami; Beata Shiratori; Motoki Usuzawa; Yasuhiko Suzuki; Toshio Hattori
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 226, 4, 313, 319, 2012年04月, ［査読有り］
英語, 研究論文（学術雑誌） - Possible horizontal transmission of Salmonella via reusable egg trays in Thailand
Fuangfa Utrarachkij; Srirat Pornraungwong; Kanokrat Siripanichgon; Chie Nakajima; Yasuhiko Suzuki; Orasa Suthienkul
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 154, 1-2, 73, 78, 2012年03月, ［査読有り］
英語, 研究論文（学術雑誌） - Amino Acid Substitutions at Position 95 in GyrA Can Add Fluoroquinolone Resistance to Mycobacterium leprae
Kazumasa Yokoyama; Hyun Kim; Tetsu Mukai; Masanori Matsuoka; Chie Nakajima; Yasuhiko Suzuki
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56, 2, 697, 702, 2012年02月, ［査読有り］
英語, 研究論文（学術雑誌） - Influence of Lineage-Specific Amino Acid Dimorphisms in GyrA on Mycobacterium tuberculosis Resistance to Fluoroquinolones
Hyun Kim; Chie Nakajima; Youn Uck Kim; Kazumasa Yokoyama; Yasuhiko Suzuki
JAPANESE JOURNAL OF INFECTIOUS DISEASES, 65, 1, 72, 74, 2012年01月, ［査読有り］
英語, 研究論文（学術雑誌） - Impact of the E540V Amino Acid Substitution in GyrB of Mycobacterium tuberculosis on Quinolone Resistance
Hyun Kim; Chie Nakajima; Kazumasa Yokoyama; Zeaur Rahim; Youn Uck Kim; Hiroki Oguri; Yasuhiko Suzuki
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 55, 8, 3661, 3667, 2011年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Genotypes and Characteristics of Clustering and Drug Susceptibility of Mycobacterium tuberculosis Isolates Collected in Heilongjiang Province, China
Juan Wang; Yan Liu; Chun-Lei Zhang; Bin-Ying Ji; Liu-Zhuo Zhang; Yong-Zhen Shao; Shui-Lian Jiang; Yasuhiko Suzuki; Chie Nakajima; Chang-Long Fan; Yuan-Ping Ma; Geng-Wen Tian; Toshio Hattori; Hong Ling
JOURNAL OF CLINICAL MICROBIOLOGY, 49, 4, 1354, 1362, 2011年04月, ［査読有り］
英語, 研究論文（学術雑誌） - Molecular mechanism of the acquisition of new-quinolone resistance in mycobacterium leprae and M. Tuberculosis and rapid differentiation methods for resistant bacilli
Hyun Kim; Haruka Suzuki; Masanori Matsuoka; Takashi Matsuba; Kazumasa Yokoyama; Chie Nakajima; Yasuhiko Suzuki
Japanese Journal of Leprosy, 80, 1, 17, 27, 1, 2011年, ［査読有り］
英語, 研究論文（学術雑誌） - Rapid detection of Mycobacterium tuberculosis complex in Cattle and Lechwe (Kobus leche kafuensis) at the slaughter house.
Hang’ombe M B; Nakajima C; Ishii A; Fukushima Y; Munyeme M; Matandiko W; Mweene A S; Suzuki Y
Vet. Sci. Dev., 1, e5, 2011年, ［査読有り］
英語, 研究論文（学術雑誌） - [Envelope structure and components of Mycobacterium tuberculosis].
Matsuba T; Nakajima C; Suzuki Y
Nihon saikingaku zasshi. Japanese journal of bacteriology, 65, 3, 355, 368, 2-4, 2010年12月, ［査読有り］ - Possible Mode of Emergence for Drug-Resistant Leprosy Is Revealed by an Analysis of Samples from Mexico
Masanori Matsuoka; Yasuhiko Suzuki; Iris Estrada Garcia; Mary Fafutis-Morris; Alberto Vargas-Gonzalez; Cristina Carreno-Martinez; Yukari Fukushima; Chie Nakajima
JAPANESE JOURNAL OF INFECTIOUS DISEASES, 63, 6, 412, 416, 2010年11月, ［査読有り］
英語, 研究論文（学術雑誌） - Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital
Kanako Ishihara; Natsumi Shimokubo; Akie Sakagami; Hiroshi Ueno; Yasukazu Muramatsu; Tsuyoshi Kadosawa; Chie Yanagisawa; Hideaki Hanaki; Chie Nakajima; Yasuhiko Suzuki; Yutaka Tamura
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76, 15, 5165, 5174, 2010年08月, ［査読有り］
英語, 研究論文（学術雑誌） - A Novel Method for the Purification of DNA by Capturing Nucleic Acid and Magnesium Complexes on Non-Woven Fabric Filters under Alkaline Conditions for the Gene Diagnosis of Tuberculosis by Loop-Mediated Isothermal Amplification (LAMP)
Tadashi Fukasawa; Naozumi Oda; Yasunao Wada; Aki Tamaru; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki
JAPANESE JOURNAL OF INFECTIOUS DISEASES, 63, 4, 246, 250, 2010年07月, ［査読有り］
英語, 研究論文（学術雑誌） - Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR
Chie Nakajima; Zeaur Rahim; Yukari Fukushima; Isamu Sugawara; Adri G. M. van der Zanden; Aki Tamaru; Yasuhiko Suzuki
BMC INFECTIOUS DISEASES, 10, 118, 2010年05月, ［査読有り］
英語, 研究論文（学術雑誌） - Zoonotic aspects of tuberculosis caused by mycobacterium bovis
Yasuhiko Suzuki; Takashi Matsuba; Chie Nakajima
Kekkaku, 85, 2, 79, 86, 2, 2010年, ［査読有り］
日本語, 研究論文（国際会議プロシーディングス） - Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis
Saiki Imamura; Itabajara da Silva Vaz; Satoru Konnai; Shinji Yamada; Chie Nakajima; Misao Onuma; Kazuhiko Ohashi
EXPERIMENTAL AND APPLIED ACAROLOGY, 48, 4, 345, 358, 2009年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Suppression of cell proliferation and cytokine expression by HL-p36, a tick salivary gland-derived protein of Haemaphysalis longicornis
Satoru Konnai; Chie Nakajima; Saiki Imamura; Shinji Yamada; Hideto Nishikado; Michi Kodama; Misao Onuma; Kazuhiko Ohashi
IMMUNOLOGY, 126, 2, 209, 219, 2009年02月, ［査読有り］
英語, 研究論文（学術雑誌） - Detection of culturable and nonculturable Legionella species from hot water systems of public buildings in Japan
A. Edagawa; A. Kimura; H. Doi; H. Tanaka; K. Tomioka; K. Sakabe; C. Nakajima; Y. Suzuki
JOURNAL OF APPLIED MICROBIOLOGY, 105, 6, 2104, 2114, 2008年12月, ［査読有り］
英語, 研究論文（学術雑誌） - A novel method for simple detection of mutations conferring drug resistance in Mycobacterium leprae, based on a DNA microarray, and its applicability in developing countries
Masanori Matsuoka; Khin Saw Aye; Kyaw Kyaw; Esterlina Virtudes Tan; Ma Victoria Balagon; Paul Saunderson; Robert Gelber; Masanao Makino; Chie Nakajima; Yasuhiko Suzuki
JOURNAL OF MEDICAL MICROBIOLOGY, 57, 10, 1213, 1219, 2008年10月, ［査読有り］
英語, 研究論文（学術雑誌） - Effects of anti-tick cocktail vaccine against Rhipicephalus appendiculatus
Saiki Imamura; Satoru Konnai; Itabajara da Silva Vaz Junior; Shinji Yamada; Chie Nakajima; Yuko Ito; Tomoko Tajima; Jun Yasuda; Martin Simuunza; Misao Onuma; Kazuhiko Ohashi
JAPANESE JOURNAL OF VETERINARY RESEARCH, 56, 2, 85, 98, 2008年08月, ［査読有り］
英語, 研究論文（学術雑誌） - Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients
Basu Dev Pandey; Ajay Poudel; Tomoko Yoda; Aki Tamaru; Naozumi Oda; Yukari Fukushima; Binod Lekhak; Basista Risal; Bishnu Acharya; Bishwa Sapkota; Chie Nakajima; Tooru Taniguchi; Benjawan Phetsuksiri; Yasuhiko Suzuki
JOURNAL OF MEDICAL MICROBIOLOGY, 57, 4, 439, 443, 2008年04月, ［査読有り］
英語, 研究論文（学術雑誌） - Loss of a conserved 7-methylguanosine modification in 16S rRNA confers low-level streptomycin resistance in bacteria
Susumu Okamoto; Aki Tamaru; Chie Nakajima; Kenji Nishimura; Yukinori Tanaka; Shinji Tokuyama; Yasuhiko Suzuki; Kozo Ochi
MOLECULAR MICROBIOLOGY, 63, 4, 1096, 1106, 2007年02月, ［査読有り］
英語, 研究論文（学術雑誌） - Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus
Satoru Konnai; Saiki Imamura; Chie Nakajima; William Harold Witola; Shinji Yamada; Martin Simuunza; Andrew Nambota; Jun Yasuda; Kazuhiko Ohashi; Misao Onuma
ACTA TROPICA, 99, 1, 34, 41, 2006年08月, ［査読有り］
英語, 研究論文（学術雑誌） - A novel gene encoding a thrombin inhibitory protein in a cDNA library from Haemaphysalis longicornis salivary gland
C Nakajima; S Imamura; S Konnai; S Yamada; H Nishikado; K Ohashi; M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, 5, 447, 452, 2006年05月, ［査読有り］
英語, 研究論文（学術雑誌） - Random sequencing of cDNA library derived from partially-fed adult female Haemaphysalis longicornis salivary gland
C Nakajima; ID Vaz; S Imamura; S Konnai; K Ohashi; M Onuma
JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 11, 1127, 1131, 2005年11月, ［査読有り］
英語, 研究論文（学術雑誌） - Loop-mediated isothermal amplification for rapid detection of Newcastle disease virus
HM Pham; C Nakajima; K Ohashi; M Onuma
JOURNAL OF CLINICAL MICROBIOLOGY, 43, 4, 1646, 1650, 2005年04月, ［査読有り］
英語, 研究論文（学術雑誌） - Molecular cloning and sequence analysis of cDNAs encoding for Boophilus microplus, Haemaphysalis longicornis and Rhipicephalus appendiculatus actins
ID Vaz; S Imamura; C Nakajima; FC de Cardoso; CAS Ferreira; G Renard; A Masuda; K Ohashi; N Onuma
VETERINARY PARASITOLOGY, 127, 2, 147, 155, 2005年01月, ［査読有り］
英語, 研究論文（学術雑誌） - A scientific note on in vitro experimental infection of American foulbrood in honeybees Apis mellifera L.
T Sakogawa; S Kataoka; A Okayama; C Nakajima; T Hayama
APIDOLOGIE, 30, 1, 75, 75, 1999年01月, ［査読有り］
英語, 研究論文（学術雑誌） - Disposition of mirosamicin in honeybee hives.
C Nakajima; T Sakogawa; A Okayama; A Nakamura; T Hayama
Journal of veterinary pharmacology and therapeutics, 21, 4, 269, 73, 1998年08月, ［査読有り］, ［国際誌］
英語, 研究論文（学術雑誌） - Sporicidal Activities of Disinfectants on Paenibacillus larvae
Atsuko Okayama; Tomoko Sakogawa; Chie Nakajima; Toyoaki Hayama
Journal of Veterinary Medical Science, 59, 10, 953, 954, 1997年10月
研究論文（学術雑誌） - Disposition of Ampicillin in Honeybees and Hives
Chie Nakajima; Atsuko Okayama; Tomoko Sakogawa; Akira Nakamura; Toyoaki Hayama
Journal of Veterinary Medical Science, 59, 9, 765, 767, 1997年09月
研究論文（学術雑誌） - Biological properties and antibiotic susceptibility of bacillus larvae originated from American foulbrood of honeybee in Japan
Atsuko Okayama; Tomoko Sakogawa; Chie Nakajima; Toyoaki Hayama
Journal of Veterinary Medical Science, 58, 5, 439, 441, 1996年05月
研究論文（学術雑誌） - Residue analysis of spectinomycin in chicken and swine tissues by HPLC
Katsushi Ohguchi; Hiromi Takahara; Mariko Matsumoto; Chie Nakajima; Yasumasa Kido
Journal of the Food Hygienic Society of Japan, 37, 5, 319, 324, 1996年
研究論文（学術雑誌）
- ライ菌のDNAジャイレースの温度と活性の関係に関する解析(Analysis of the relationship between temperature and activity of Mycobacterium leprae DNA gyrase)
八代 龍; 金 玄; Suwanthada Pondpan; Poussier Chariotte; 中島 千絵; 鈴木 定彦; 阿戸 学; 中田 登, 日本細菌学雑誌, 80, 2, 107, 107, 2025年04月
日本細菌学会, 英語 - 国境を越えて:留学生・ポスドクの挑戦 2016年から2020年までのタイの運河水におけるSalmonellaの遺伝的多様性と抗菌薬耐性(Beyond Borders: Challenges for International Students and Postdocs Genetic diversity and antimicrobial resistance of Salmonella in Thai canal water from 2016 to 2020)
Toyting Jirachaya; 佐藤 豊孝; Supha Neunghatai; Thongpanich Yuwanda; 堀内 基広; Thapa Jeewan; 中島 千絵; 鈴木 定彦; Utrarachkij Fuangfa, 日本細菌学雑誌, 80, 2, 34, 34, 2025年04月
日本細菌学会, 英語 - Mycobacterium shinjukuenseにおける遺伝的多様性とゲノム安定性
和田 崇之; 井上 陽晴; 吉田 志緒美; 村瀬 良朗; 中島 千絵; 鈴木 定彦; 御手洗 聡; 福島 由華理, 日本細菌学雑誌, 80, 2, 88, 88, 2025年04月
日本細菌学会, 日本語 - ライ菌のDNAジャイレースの温度と活性の関係に関する解析(Analysis of the relationship between temperature and activity of Mycobacterium leprae DNA gyrase)
八代 龍; 金 玄; Suwanthada Pondpan; Poussier Chariotte; 中島 千絵; 鈴木 定彦; 阿戸 学; 中田 登, 日本細菌学雑誌, 80, 2, 107, 107, 2025年04月
日本細菌学会, 英語 - イヌ常在菌Staphylococcus pseudintermedius由来SpsQとイヌIgGとの結合性の評価
竹内寛人; 竹内寛人; 中島千絵; 今内覚; 今内覚; 今内覚; 前川直也; 岡川朋弘; 岡川朋弘; 臼井優; 田村豊; 鈴木定彦; 村田史郎; 大橋和彦; 鈴木定彦; 村田史郎; 大橋和彦, 日本獣医学会学術集会講演要旨集, 168th, 2025年 - 比較ゲノム解析による乳牛由来黄色ブドウ球菌の国際的な拡散状況の解明
大川 佳穂; 福田 昭; 米山 裕; 戸部 隆太; 中島 千絵; 鈴木 定彦; 臼井 優, 北海道獣医師会雑誌, 68, 8, 329, 329, 2024年08月
(公社)北海道獣医師会, 日本語 - WQ-3810: A Novel Fluoroquinolone Exhibiting Potency Against Fluoroquinolone-Resistant M.avium(タイトル和訳中)
Jayaweera Sasini; Thapa Jeewan; Nakajima Chie; Suzuki Yasuhiko, 日本細菌学雑誌, 79, 2, 268, 268, 2024年07月
日本細菌学会, 英語 - タイ、バンコクの運河の水から分離されたサルモネラ菌のゲノム解析(Genomic Analysis of Salmonella Isolated from Canal Water in Bangkok, Thailand)
Toyting Jirachaya; Nuanmuang Narong; Utrarachkij Fuangfa; Leekitcharoenphon Pimlapas; Aarestrup Frank; 佐藤 豊孝; Thapa Jeewan; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 79, 2, 128, 128, 2024年06月
日本細菌学会, 英語 - 日本の農場における家畜堆肥由来腸球菌の可動性リネゾリド耐性遺伝子(Mobile linezolid resistance genes in enterococci derived from livestock compost at Japanese farms)
福田 昭; 中島 千絵; 鈴木 定彦; 臼井 優, 日本細菌学雑誌, 79, 2, 121, 121, 2024年06月
日本細菌学会, 英語 - Mobile linezolid resistance genes in enterococci derived from livestock compost at Japanese farms(タイトル和訳中)
福田 昭; 中島 千絵; 鈴木 定彦; 臼井 優, 日本細菌学雑誌, 79, 2, 121, 121, 2024年06月
日本細菌学会, 英語 - Genomic Analysis of Salmonella Isolated from Canal Water in Bangkok, Thailand(タイトル和訳中)
Toyting Jirachaya; Nuanmuang Narong; Utrarachkij Fuangfa; Leekitcharoenphon Pimlapas; Aarestrup Frank; 佐藤 豊孝; Thapa Jeewan; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 79, 2, 128, 128, 2024年06月
日本細菌学会, 英語 - デラフロキサシンのらい菌DNAジャイレースに対する作用(第2報)
Eomseob Jang; Pondpan Suwanthada; 中島 千絵; 阿戸 学; 向井 徹; 鈴木 定彦, 日本ハンセン病学会雑誌, 93, 1, 21, 21, 2024年04月
日本ハンセン病学会, 日本語 - アルカリ処理によるポリマーピエゾバイオセンサ素子の表面改質
村田佳菜子; 小林祐騎; 荻谷泰斗; 武田真理子; 秀島翔; 宗像文男; 中島千絵; 鈴木定彦, センサ・マイクロマシンと応用システムシンポジウム(CD-ROM), 41st, 2024年 - 世界の結核研究の動向(40)結核菌の薬剤耐性と遺伝子変異について
中島千絵; 鈴木定彦, 複十字, 415, 2024年 - 鼻疽菌検出のための新規dry LAMP法の開発
中瀬 満; Thapa Jeewan; Batbaatar Vanaabaatar; Khurtsbaatar Ochirbat; Enkhtuul Batchuluun; Unenbat Jugderkhorloo; 中島 千絵; 鈴木 定彦; 木村 享史, 日本獣医学会学術集会講演要旨集, 166回, 116, 116, 2023年09月
(公社)日本獣医学会, 日本語 - デラフロキサシンのらい菌DNAジャイレースに対する作用
鈴木 定彦; 朴 鐘勲; Pondpan Suwanthada; 中島 千絵, 日本ハンセン病学会雑誌, 92, 1, 23, 23, 2023年04月
日本ハンセン病学会, 日本語 - タイの運河由来Salmonella属におけるプラスミドを介したキノロン系薬耐性遺伝子の有病率(Prevalence of plasmid-mediated quinolone resistance genes in Salmonella spp. from canals of Thailand)
Toyting Jirachaya; Utrarachkij Fuangfa; Supha Neunghatai; Thongpanich Yuwanda; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 78, 1, 70, 70, 2023年02月
日本細菌学会, 英語 - SalmonellaTyphimuriumのフルオロキノロン耐性に及ぼすGyrAおよびQnrB19のアミノ酸置換の影響(Impact of mutations in GyrA and QnrB19 on resistance to fluoroquinolone in Salmonella Typhimurium)
Suwanthada Pondpan; Thapa Jeewan; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 78, 1, 105, 105, 2023年02月
日本細菌学会, 英語 - Mycobacterium avium gyrAにおけるフルオロキノロン耐性関連変異が耐性へ果たす役割(Role of fluoroquinolone resistance-associated mutations in Mycobacterium avium gyrA to resistance)
Thapa Jeewan; Chizimu Joseph Yamweka; 北村 そよか; Akapelwa Mwangala Lonah; Suwanthada Pondpan; 三浦 菜実; Toyting Jirachaya; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 78, 1, 105, 105, 2023年02月
日本細菌学会, 英語 - タイにおける豚からヒトへのLA-MRSAの人獣共通感染の全ゲノム解析(Whole Genome Analysis of Zoonotic Transmission of LA-MRSA from Pigs to Humans in Thailand)
Narongpun Pawarut; チャンチャイトン・パッタラット; 山岸 潤也; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 78, 1, 109, 109, 2023年02月
日本細菌学会, 英語 - 日本で分離されたM.avium臨床分離株におけるLevofloxacinのMICに対するmfpAをエンコードするPRPの影響(The effect of mfpA encoding PRP on the MICs of Levofloxacin in M.avium clinical isolates from Japan)
Akapelwa Mwangala; 会津 勇樹[大内]; Chizimu Joseph Yamweka; Kapalamula Thoko Flav; Menda Conscilliah Rhombohl; 西内 由紀子; 鈴木 定彦; 中島 千絵, 日本細菌学雑誌, 78, 1, 151, 151, 2023年02月
日本細菌学会, 英語 - 伴侶動物由来メチシリン耐性Staphylococcus pseudintermediusの現状と人獣共通感染症の可能性
盛田佐和; 福田昭; 岡村幸一; 小松忠人; 露木勇三; 鳥居花衣; 佐々木潤平; 鈴木定彦; 中島千絵; 臼井優, 動物用抗菌剤研究会報, 45, 2023年 - 鼻疽菌検出のための新規dry LAMP法の開発
中瀬満; THAPA Jeewan; BATBAATAR Vanaabaatar; KHURTSBAATAR Ochirbat; ENKHTUUL Batchuluun; UNENBAT Jugderkhorloo; 中島千絵; 鈴木定彦; 木村享史, 日本獣医学会学術集会講演要旨集, 166th, 116, 116, 2023年
(公社)日本獣医学会, 日本語 - 消毒薬が抗菌薬耐性プラスミドの選択圧となる可能性の解明
川端 結; 石川 北斗; 大枝 夏希; 浅井 鉄夫; 中島 千絵; 鈴木 定彦; 福田 昭; 臼井 優, 日本獣医学会学術集会講演要旨集, 165回, [F1P, 03], 2022年09月
(公社)日本獣医学会, 日本語 - 新たな置換基を1位に保有するキノロン系抗菌薬とらい菌DNAジャイレースの相互作用
朴 鐘勲; 山口 智之; 会津 勇樹[大内]; 小出 健太郎; Pachanon Ruttana; Chizimu Joseph Yamweka; 森 茂太郎; 金 玄; 向井 徹; 中島 千絵; 鈴木 定彦, 日本ハンセン病学会雑誌, 91, 1, 27, 27, 2022年05月
日本ハンセン病学会, 日本語 - 低温調理における耐熱性細菌・非耐熱性細菌の死滅条件の解明
中野瞳; 福田昭; 中島千絵; 鈴木定彦; 臼井優, 北海道獣医師会雑誌, 66, 8, 301, 301, 2022年
(公社)北海道獣医師会, 日本語 - 焼成ホタテ貝殻粉末と石灰窒素による堆肥中の薬剤耐性菌制御の可能性
江浪 誠俊; 福田 昭; 中島 千絵; 鈴木 定彦; 臼井 優, 日本獣医学会学術集会講演要旨集, 164回, [FO, 15], 2021年09月
(公社)日本獣医学会, 日本語 - 市販堆肥における薬剤耐性菌/耐性遺伝子の分布状況の解明
小南 風佳; 福田 昭; 中島 千絵; 鈴木 定彦; 臼井 優, 日本獣医学会学術集会講演要旨集, 164回, [FO, 35], 2021年09月
(公社)日本獣医学会, 日本語 - 抗菌薬の繰り返し曝露がClostridioides difficileの性状に与える影響の解明
鈴木 和総; 福田 昭; 中島 千絵; 鈴木 定彦; 臼井 優, 日本獣医学会学術集会講演要旨集, 164回, [FO, 37], 2021年09月
(公社)日本獣医学会, 日本語 - 環境からヒトの生活に侵入する病原微生物 人獣共通感染症としての結核と非定型抗酸菌症
中島 千絵; 鈴木 定彦, 日本化学療法学会雑誌, 69, 1, 50, 50, 2021年01月
(公社)日本化学療法学会, 日本語 - 動物用抗体医薬品の開発および臨床研究
今内 覚; 岡川 朋弘; 前川 直也; 中島 千絵; 鈴木 定彦; 山本 啓一; 戸田 幹洋; 村田 史郎; 大橋 和彦, JVPA digest : 日本動物用医薬品協会会報, 67, 1, 10, 2020年02月
日本動物薬事協会, 日本語 - 等温増幅とDNAクロマトグラフィーによるリファンピシン耐性結核菌の多重変異検出方の開発
宝田裕; 小寺拓也; 小林久美; 中島千絵; 中島千絵; 川瀬三雄; 鈴木定彦; 鈴木定彦, 日本分子生物学会年会プログラム・要旨集(Web), 43rd, 2020年 - タイ国のブタとブタ肉から分離されたMRSAの分子的特性と抗菌薬耐性(Molecular characterization and antimicrobial resistance of MRSA from pigs and pork in Thailand)
Tanomsridachchai Wimonrat; 中島 千絵; Changkaew Kanjana; Changkwanyeun Ruchirada; Prapasawat Watsawan; Intarapuk Apiradee; Yamasamit Nattapong; Suthienkul Orasa; 鈴木 定彦, 日本細菌学雑誌, 75, 1, 60, 60, 2020年01月
日本細菌学会, 英語 - フィリピンの食用動物から分離されたE.coliのキノロン耐性決定因子の検出(Detection of quinolone resistance determinants in E. coli from food animals in the Philippines)
Belotindos Lawrence; Mingala Claro; Villanueva Marvin; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 75, 1, 64, 64, 2020年01月
日本細菌学会, 英語 - 病原性抗酸菌における細胞外DNAの存在と抗酸菌の生理におけるその役割(Existence of extracellular DNA in pathogenic mycobacteria and its role in mycobacterial physiology)
イリノフ・アレクサンドル; シャバン・アミナ; 袴田 真理子; 西山 晃史; 尾関 百合子; 福島 由華里; 中島 千絵; 立石 善隆; 鈴木 定彦; 松本 壮吉, 日本細菌学雑誌, 75, 1, 74, 74, 2020年01月
日本細菌学会, 英語 - 水平ゲノム転移を介したvanD5バンコマイシン耐性遺伝子を保有するEnterococcus faeciumの出現(Emergence of vanD5-type vancomycin-resistant Enterococcus faecium via horizontal genomic transfer)
佐藤 豊孝; 和田 崇之; 福島 由華里; 中島 千絵; 鈴木 定彦; 高橋 聡; 横田 伸一, 日本細菌学雑誌, 75, 1, 100, 100, 2020年01月
日本細菌学会, 英語 - Molecular characterization and antimicrobial resistance of MRSA from pigs and pork in Thailand(和訳中)
Tanomsridachchai Wimonrat; 中島 千絵; Changkaew Kanjana; Changkwanyeun Ruchirada; Prapasawat Watsawan; Intarapuk Apiradee; Yamasamit Nattapong; Suthienkul Orasa; 鈴木 定彦, 日本細菌学雑誌, 75, 1, 60, 60, 2020年01月
日本細菌学会, 英語 - Detection of quinolone resistance determinants in E. coli from food animals in the Philippines(和訳中)
Belotindos Lawrence; Mingala Claro; Villanueva Marvin; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 75, 1, 64, 64, 2020年01月
日本細菌学会, 英語 - Existence of extracellular DNA in pathogenic mycobacteria and its role in mycobacterial physiology(和訳中)
イリノフ・アレクサンドル; シャバン・アミナ; 袴田 真理子; 西山 晃史; 尾関 百合子; 福島 由華里; 中島 千絵; 立石 善隆; 鈴木 定彦; 松本 壮吉, 日本細菌学雑誌, 75, 1, 74, 74, 2020年01月
日本細菌学会, 英語 - Emergence of vanD5-type vancomycin-resistant Enterococcus faecium via horizontal genomic transfer(和訳中)
佐藤 豊孝; 和田 崇之; 福島 由華里; 中島 千絵; 鈴木 定彦; 高橋 聡; 横田 伸一, 日本細菌学雑誌, 75, 1, 100, 100, 2020年01月
日本細菌学会, 英語 - Direct detection of Mycobacterium tuberculosis in clinical samples by a dry methyl green loop-mediated isothermal amplification (LAMP) method
Jeewan Thapa; Bhagwan Maharjan; Meena Malla; Yukari Fukushima; Ajay Poudel; Basu Dev Pandey; Kyoko Hyashida; Stephen V. Gordon; Chie Nakajima; Yasuhiko Suzuki, Tuberculosis, 117, 1, 6, 2019年07月01日, ［国際誌］
英語 - 非結核性抗酸菌(MAC及びM.kansasii)の新規核酸増幅検査法の開発
橋本章司; 新井剛; 高田宏宗; 韓由紀; 田村嘉孝; 永井崇之; 松井謹; 小野原健一; 吉多仁子; 中島千絵; 鈴木定彦, 結核, 94, 3, 311, 2019年03月15日
日本語 - One Healthアプローチを通じた細菌性感染症の制御 病原性微生物の特性に関する新規分析法の開発(Development of new methods for the characterization of bacterial pathogens)
中島 千絵; 鈴木 定彦; Thapa Jeewan, 日本細菌学雑誌, 74, 1, 5, 5, 2019年03月
日本細菌学会, 英語 - スリランカ、Kandyにおける結核菌の集団構成 欧米系統の優勢(Population structure of Mycobacterium tuberculosis in Kandy, Sri Lanka: Dominance of Euro-American lineage)
Mendis Charitha; Ratnatunga Champa; Thevanesam Vasanthi; Kumara Athula; Wickramasinghe Susiji; Madagedara Dushantha; Gamage Chandika; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 45, 45, 2019年03月
日本細菌学会, 英語 - タイ国の屠殺場と市場から分離されたMRSAの分子的分析(Molecular analysis of MRSA isolates from slaughterhouses and markets in Thailand)
Tanomsridachchai Wimonrat; Changkaew Kanjana; Changkwanyeun Ruchirada; 中島 千絵; Suthienkul Orasa; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 45, 45, 2019年03月
日本細菌学会, 英語 - ループ仲介等温増幅法を用いた人畜共通感染性結核の迅速検出法(Rapid detection of zoonotic tuberculosis using Loop mediated isothermal amplification)
Kapalamula Thoko; Thapa Jeewan; 中島 千絵; Akapelwa Mwangala; Gordon Stephen V; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 67, 67, 2019年03月
日本細菌学会, 英語 - ループ仲介等温増幅法を用いたMycobacterium aviumの迅速検出ツールの開発(Development of a rapid detection tool for Mycobacterium avium using Loop-mediated isothermal Amplification)
Akapelwa Mwangala; Kapalamula Thoko; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 68, 68, 2019年03月
日本細菌学会, 英語 - ザンビア、Lusakaで分離された結核菌のpyrazinamide耐性の分析(Determination of pyrazinamide resistance in Mycobacterium tuberculosis isolated from Lusaka, Zambia)
Bwalya Precious; Yamaguchi Tomoyuki; Mulundu Georgina; 中島 千絵; Mbulo Grace; Solo Eddie; Fukushima Yukari; Kasakwa Kunda; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 79, 79, 2019年03月
日本細菌学会, 英語 - バイオフィルム中における大腸菌persister制御のためのトスフロキサシンとSOS反応阻害剤の可能性
臼井 優; 横尾 勇人; 田村 豊; 中島 千絵; 鈴木 定彦; Jean-Marc Ghigo; Beloin Christophe, 日本細菌学雑誌, 74, 1, 80, 80, 2019年03月
日本細菌学会, 日本語 - WQ-3810はMycobacterium lepraeのDNAジャイレースに対して強力な阻害活性を示した(WQ-3810 showed strong inhibitory activity against Mycobacterium leprae DNA gyrase)
Park JongHoon; 山口 智之; 大内 勇樹; Kim Hyun; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 82, 82, 2019年03月
日本細菌学会, 英語 - SOS応答を標的とした薬剤耐性制御物質としてのプロテアーゼ阻害剤の可能性
横尾 勇人; 臼井 優; 鈴木 定彦; 中島 千絵; 田村 豊, 日本細菌学雑誌, 74, 1, 99, 99, 2019年03月
日本細菌学会, 日本語 - プラスミドにコードされるキノロン耐性タンパク質QnrB19とサルモネラDNAジャイレースの相互作用(Interaction of the plasmid-encoded quinolone resistance protein QnrB19 with salmonella DNA gyrases)
Pachanon Ruttana; 小出 健太郎; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 99, 99, 2019年03月
日本細菌学会, 英語 - タイ水圏環境中から分離された多剤耐性大腸菌の遺伝子学的特徴(Genomic characterization of multidrug resistant E. coli in environmental water in Thailand)
角田 梨紗; 中島 千絵; 臼井 優; 田村 豊; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 100, 100, 2019年03月
日本細菌学会, 英語 - キノロン耐性結核菌におけるFitness Costおよびその補完機構(Fitness Cost and Compensatory Mechanism in Fluoroquinolone Resistance Mycobacterium tuberculosis)
大内 勇樹; 向井 徹; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 101, 101, 2019年03月
日本細菌学会, 英語 - Mycobacterium lepraeのDNAジャイレースの機能的分析と細菌増殖と生存における役割(Functional analysis of Mycobacterium leprae DNA gyrase and its role in bacterial growth and survival)
金 玄; 福富 康夫; 中島 千絵; Kim Youn Uck; 森 茂太郎; 柴山 恵吾; 中田 登; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 121, 121, 2019年03月
日本細菌学会, 英語 - フィリピンの食用動物から分離されたE.coliとSalmonellaにおけるキノロン耐性の決定因子(Quinolone Resistance Determinants in E. coli and Salmonella from Food animals in the Philippines)
Belotindos Lawrence; Mingala Claro; Villanueva Marvin; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 74, 1, 129, 129, 2019年03月
日本細菌学会, 英語 - バイオフィルム中における大腸菌persister制御のためのトスフロキサシンとSOS反応阻害剤の可能性
臼井優; 横尾勇人; 田村豊; 中島千絵; 鈴木定彦; JEAN-MARC Ghigo; BELOIN Christophe, 日本細菌学雑誌(Web), 74, 1, 2019年 - SOS応答を標的とした薬剤耐性制御物質としてのプロテアーゼ阻害剤の可能性
横尾勇人; 臼井優; 鈴木定彦; 中島千絵; 田村豊, 日本細菌学雑誌(Web), 74, 1, 2019年 - 免疫学的解析を基盤とした動物用創薬研究
今内覚; 岡川朋弘; 前川直也; 中島千絵; 中島千絵; 鈴木定彦; 鈴木定彦; 山本啓一; 山本啓一; 戸田幹洋; 戸田幹洋; 村田史郎; 大橋和彦, 臨床獣医, 37, 3, 37‐41, 2019年
日本語 - 【結核・非結核性抗酸菌症-エキスパートが教える 実臨床に役立つ最新知見】結核・非結核性抗酸菌症の基礎研究 結核菌の薬剤耐性獲得
山口 智之; 中島 千絵; 鈴木 定彦, 呼吸器ジャーナル, 66, 4, 650, 656, 2018年11月
(株)医学書院, 日本語 - 動物難治性疾病に対する創薬研究
今内 覚; 岡川朋弘; 前川直也; 中島千絵; 鈴木定彦; 山本啓一; 戸田幹洋; 村田史郎; 大橋和彦, MPアグロジャーナル, 35, 10, 22, 25, 2018年10月, ［招待有り］
MPアグロ, 日本語, 記事・総説・解説・論説等（学術雑誌） - ポリマー圧電素子を用いた病原体特異的分子検出用バイオセンサの開発―分子識別素子の設計―
武田真理子; 平田孝道; 黒岩崇; 中島千絵; 鈴木定彦; 宗像文男, Chemical Sensors, 34, Supplement A, 37‐39, 39, 2018年03月09日
電気化学会化学センサ研究会, 日本語 - ポリマー圧電素子を用いた病原体特異的分子検出用バイオセンサの開発―検知用バイオセンサの試作と評価―
武田真理子; 平田孝道; 黒岩崇; 中島千絵; 鈴木定彦; 宗像文男, 電気化学会大会講演要旨集(CD-ROM), 85th, ROMBUNNO.PS‐93, 2018年02月23日
日本語 - ポリマー圧電素子を用いた病原体特異的分子検出用バイオセンサの開発―分子識別素子の設計―
武田真理子; 平田孝道; 黒岩崇; 中島千絵; 鈴木定彦; 宗像文男, 電気化学会大会講演要旨集(CD-ROM), 85th, ROMBUNNO.1B13, 2018年02月23日
日本語 - ミャンマーにおける北京型結核菌の考察(Insight into the Beijing genotype Mycobacterium tuberculosis in Myanmar)
Lai Lai San; Nan Aye Thida Oo; Wah Wah Aung; Khin Saw Aye; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 73, 1, 50, 50, 2018年02月
日本細菌学会, 英語 - GyrAに新規アミノ酸置換を有するSalmonella enteritidisの全ゲノムシークエンス分析(Whole genome sequencing of Salmonella Enteritidis having a novel amino acid substitution on GyrA)
小出 健太郎; Utrarachkil Fuangfa; Suthienkul Orasa; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 73, 1, 51, 51, 2018年02月
日本細菌学会, 英語 - ミャンマーで分離された結核菌における薬剤耐性関連変異の同定(Detection of drug-resistant associated mutations in Mycobacterium tuberculosis isolates of Myanmar)
Nan Aye Thida Oo; Lai Lai San; Khin Saw Aye; Wah Wah Aung; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 73, 1, 55, 55, 2018年02月
日本細菌学会, 英語 - メチルグリーンを用いたループ仲介等温増幅法による結核の診断(Diagnosis of tuberculosis by methyl green based loop-mediated isothermal amplification method)
Thapa Jeewan; Maharjan Bhagwan; 福島 由華里; Malla Meena; Poudel Ajay; Pandey Basu Dev; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 73, 1, 64, 64, 2018年02月
日本細菌学会, 英語 - BCGの増殖率促進に関与する遺伝子の調査(Investigation of genes involved in promoting the growth rate of BCG)
竜門 亜矢子; 中山 真彰; 和田 崇之; 橘 理人; 阿戸 学; 中島 千絵; 鈴木 定彦; 小崎 弘貴; 大原 直也, 日本細菌学雑誌, 73, 1, 68, 68, 2018年02月
日本細菌学会, 日本語 - Ofloxancin耐性Mycobacterium lepraeのDNAジャイレースに対するWQ-3810の阻害活性(Inhibitory activity of WQ-3810 against DNA gyrases of ofloxacin-resistant Mycobacterium leprae)
Park JongHoon; 山口 智之; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 73, 1, 136, 136, 2018年02月
日本細菌学会, 英語 - タイの水圏環境における薬剤耐性と残留抗菌薬濃度との関係
角田 梨紗; 臼井 優; 高田 秀重; 中島 千絵; 鈴木 定彦; 田村 豊, 日本細菌学雑誌, 73, 1, 139, 139, 2018年02月
日本細菌学会, 日本語 - フルオロキノロン耐性で非PGG3 Mycobacterium tuberculosisのDNAジャイレースの特徴(Characterization of DNA gyrase in fluoroquinolone-resistant non-PGG3 Mycobacterium tuberculosis)
大内 勇樹; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 73, 1, 139, 139, 2018年02月
日本細菌学会, 英語 - BCGの増殖速度の促進における遺伝子の調査
竜門亜矢子; 中山真彰; 中山真彰; 和田崇之; 橘理人; 阿戸学; 中島千絵; 鈴木定彦; 小崎弘貴; 大原直也; 大原直也, 日本細菌学雑誌(Web), 73, 1, 2018年 - タイの水圏環境における薬剤耐性と残留抗菌薬濃度との関係
角田梨紗; 臼井優; 高田秀重; 中島千絵; 鈴木定彦; 田村豊, 日本細菌学雑誌(Web), 73, 1, 2018年 - Multiplex PCR法による鶏肉からの薬剤耐性カンピロバクターの迅速検出法
松井有優; 臼井優; 中島千絵; 小野崎正修; 鈴木定彦; 田村豊, 日本獣医学会学術集会講演要旨集, 160th, 415, 2017年08月30日
日本語 - Novel Mycobacterium avium Complex Species Isolated From Black Wildebeest (Connochaetes gnou) in South Africa
P. N. Kabongo-Kayoka; C. L. Obi; C. Nakajima; Y. Suzuki; T. Hattori; J. N. Eloff; J. Wright; N. Mbelle; L. J. McGaw, TRANSBOUNDARY AND EMERGING DISEASES, 64, 3, 929, 937, 2017年06月
英語 - らい菌のキノロン系抗菌薬耐性の獲得に伴うDNAジャイレース活性変化の解析
鈴木定彦; 山口智之; 中島千絵, Japanese Journal of Leprosy, 86, 1, 50, 2017年04月22日
日本語 - ポリマー圧電素子を用いたDNA検出用バイオセンサの検知特性
武田真理子; 矢萩洸貴; 杉山龍男; 平田孝道; 中島千絵; 鈴木定彦; 宗像文男, Chemical Sensors, 33, Supplement A, 58‐60, 60, 2017年03月25日
電気化学会化学センサ研究会, 日本語 - ポリマー圧電素子を用いたDNA検出用バイオセンサの検知特性
武田真理子; 矢萩洸貴; 杉山龍男; 平田孝道; 中島千絵; 鈴木定彦; 宗像文男, 電気化学会大会講演要旨集(CD-ROM), 84th, ROMBUNNO.2U03, 60, 2017年03月17日
電気化学会化学センサ研究会, 日本語 - ヨーネ菌組換え抗原タンパクに対する実験感染牛の血清抗体反応性
松葉 隆司; 永田 礼子; 川治 聡子; 中島 千絵; 鈴木 定彦; 藤井 潤, 日本細菌学雑誌, 72, 1, 117, 117, 2017年02月
日本細菌学会, 日本語 - らい菌及び結核菌由来DNAジャイレースの性状解析
金 玄; 福富 康夫; 中島 千絵; 松岡 正典; 森 茂太郎; 柴山 恵吾; 鈴木 定彦, 日本細菌学雑誌, 72, 1, 136, 136, 2017年02月
日本細菌学会, 日本語 - ヨーネ菌組換え抗原タンパクに対する実験感染牛の血清抗体反応性
松葉 隆司; 永田 礼子; 川治 聡子; 中島 千絵; 鈴木 定彦; 藤井 潤, 日本細菌学雑誌, 72, 1, 117, 117, 2017年02月
日本細菌学会, 日本語 - Impact of T95S and D668G in GyrA of Mycobacterium tuberculosis on compensatory evolution(和訳中)
大内 勇樹; 小出 健太郎; 山口 智之; 朴 鐘勲; 金 玄; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 72, 1, 131, 131, 2017年02月
日本細菌学会, 英語 - Antibacterial activity of WQ-3810, a novel fluoroquinolone, against Salmonella Typhimurium(和訳中)
小出 健太郎; コングソイ・シリポン; 大内 勇樹; 朴 鐘勲; 山口 智之; チャンクワイエン・ルチラダ; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 72, 1, 135, 135, 2017年02月
日本細菌学会, 英語 - らい菌及び結核菌由来DNAジャイレースの性状解析
金 玄; 福富 康夫; 中島 千絵; 松岡 正典; 森 茂太郎; 柴山 恵吾; 鈴木 定彦, 日本細菌学雑誌, 72, 1, 136, 136, 2017年02月
日本細菌学会, 日本語 - Analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis in Nepal(和訳中)
シュレスタ・ディプティ; マハージャン・バグワン; ウーナン・エーティダ; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 72, 1, 141, 141, 2017年02月
日本細菌学会, 英語 - Molecular diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka: Insight to Beijing genotype(和訳中)
メンディス・チャリサ; Ratnatunga Champa; Thevanesam Vasanthi; Kumara Athula; Wickramasinghe Susiji; Madegedara Dushantha; Gamage Chandika; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 72, 1, 167, 167, 2017年02月
日本細菌学会, 英語 - ヨーネ菌組換え抗原タンパクに対する実験感染牛の血清抗体反応性
松葉隆司; 永田礼子; 川治聡子; 中島千絵; 鈴木定彦; 藤井潤, 日本細菌学雑誌(Web), 72, 1, 117(J‐STAGE), 2017年
日本語 - らい菌及び結核菌由来DNAジャイレースの性状解析
KIM Hyun; 福富康夫; 中島千絵; 松岡正典; 森茂太郎; 柴山恵吾; 鈴木定彦, 日本細菌学雑誌(Web), 72, 1, 136(J‐STAGE), 2017年
日本語 - 種内相互組み換えによって促進される鳥型結核菌の地域適応
矢野大和; 丸山史人; 西内由紀子; 中川一路; 中島千絵; 鈴木定彦; 岩本朋忠, 日本ゲノム微生物学会年会要旨集, 11th, 57, 2017年
日本語 - 人獣共通感染症としての結核
中島千絵; 鈴木定彦, 獣医疫学雑誌, 20, 2, 101‐104, 104, 2016年12月20日
人獣共通感染症としての観点から人型結核菌(M. tuberculosis)を見た場合，状況は上述の人における様相とはかなり異なる。毎年1千万人前後の新患を出し，世界人口の1/3が既に結核菌に感染していると言われているが，この人における重度の蔓延状態と比べると，動物におけるM. tuberculosisによる感染報告は恐ろしく少ない。霊長類や一部の例外を除いて，患者と密な接触のあった動物(牛，犬等)において稀に報告がある程度である。例外的にエチオピアにおいて牛群の集団感染が報告されているが，これはこの地域特有の人と牛との濃厚な接触習慣により，個々の牛が人から感染を受けているものと考えられる。これらに対して，大きな例外となるのが人に飼育されているアジアゾウである。最初はアメリカのサーカスや動物園で飼育されているゾウで報告され，その後，タイやネパール等のアジアゾウ本来の分布域内の飼育施設において多数の感染例が報告される様になった。我々がネパールの国立公園において行った調査では，これらのゾウ感染株はM. tuberculosisであり，ネパール患者から得られる株特有の遺伝子上の変異を持っていたことから，感染源は人間で，恐らくは日々密接な係りを持っている飼育係と考えられた。何故，アジアゾウのみから多数のM. tuberculosisによる結核発症例が報告されるのかは不明だが，菌の遅い増殖速度を考えると，動物のM. tuberculosisに対する感受性等の要因の他に，ゾウが人間並みに長命であることも関与しているかもしれない。以上より，M. tuberculosisは基本的に人の結核起因菌であり，高度に人に順化していると考えられる。世界の牛結核発生状況は人結核と趣が異なり，人結核がほとんど見られなくなった西欧諸国やニュージーランド等で高度の蔓延が観察される。また，今一つ異なる特長は，世界的に蔓延している菌株の遺伝的特徴が非常に良く似ている点である(M. tuberculosisでは地域によって多様性が見られる)。有名な例はEulと呼ばれる株で，この菌株の特徴を持った菌がアメリカ大陸やオーストラリア，ニュージーランド，南アフリカなどで分離される。型別情報からこれらの起源を辿ると，元々はイギリスやアイルランドで蔓延していた株であることが判る。これは，近代以降にこれらの国から牛が，主として大英帝国関連の国々へ輸出された名残であると推測される。南ア以外のアフリカ諸国では，また別のタイプの牛結核菌が分離されるが，これらも元を辿るとフランス，オランダ等の由来であることが示唆され，アフリカの牛結核はほぼヨーロッパが起源となっている。, 獣医疫学会, 日本語 - マイクロアレイ技術を応用した薬剤耐性カンピロバクターの迅速検出法(CAMERA法)
臼井 優; 舘野 翔; 田勢 準也; 田村 豊; 中島 千絵; 鈴木 定彦; 小野崎 正修; 大曽根 司郎, グリーンテクノ情報, 12, 2, 14, 17, 2016年09月
NPO法人グリーンテクノバンク, 日本語 - ウシ用腸溶性生菌剤カプセルが腸内細菌相に与える影響
瀬山智博; 平康博章; 吉田弦; 大沼愛子; 邱永晋; 中島千絵; 笠井浩司; 鈴木定彦, 日本畜産学会大会講演要旨, 121st, 237, 2016年03月27日
日本語 - BCG thyXを用いた抗酸菌のPAS耐性機序の解析
大原 直也; 阿戸 学; 鈴木 定彦; 小林 和夫; 有村 友紀; 中山 真彰; 中島 千絵; 妹尾 昌紀; 田川 淳平; 中山 浩次, 結核, 91, 3, 325, 325, 2016年03月
(一社)日本結核病学会, 日本語 - Mycobacterium aviumの系統分岐に伴う大規模なゲノム構造の変化
岩本 朋忠; 矢野 大和; 西内 由紀子; 有川 健太郎; 中島 千絵; 鈴木 定彦; 丸山 史人, 日本細菌学雑誌, 71, 1, 58, 58, 2016年02月
日本細菌学会, 日本語 - ミャンマーで単離された北京型多剤耐性結核菌株のMIRU-VNTRを用いた判別法(MIRU-VNTR Typing of Beijing MDR-TB strains from Myanmar)
Lai Lai San; Nan Aye Thida Oo; Wah Wah Aung; Khin Saw Aye; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 71, 1, 59, 59, 2016年02月
日本細菌学会, 英語 - ミャンマーで単離されたストレプトマイシン耐性結核菌における変異の同定(Detection of mutations in streptomycin resistance Mycobacterium tuberculosis isolates from Myanmar)
Nan Aye Thida Oo; Lai Lai San; Khin Saw Aye; Wah Wah Aung; Wah Wah; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 71, 1, 105, 105, 2016年02月
日本細菌学会, 英語 - Mycobacterium lepraeのDNAジャイレースに対する新規の8-メトキシフルオロキノロンの阻害活性(Inhibitory activities of a new 8-methoxy fluoroquinolone against DNA gyrase of Mycobacterium leprae)
山口 智之; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 71, 1, 140, 140, 2016年02月
日本細菌学会, 英語 - 抗酸菌のPAS耐性機序の解明 BCG thyX欠損株および過剰発現株の作製
有村 友紀; 中山 真彰; 妹尾 昌紀; 田川 淳平; 阿戸 学; 中島 千絵; 鈴木 定彦; 中山 浩次; 小林 和夫; 大原 直也, 日本細菌学雑誌, 71, 1, 142, 142, 2016年02月
日本細菌学会, 日本語 - 南アジアはMycobacterium orygisの風土病地域である(South Asia is an endemic region for Mycobacterium orygis)
Thapa Jeewan; 中島 千絵; Rahim Zeaur; Paudel Sarad; Shah Yogendra; Maharian Bhagwan; Poudel Ajay; Gairhe Kamal Prasad; 坪田 敏男; 鈴木 定彦, 日本細菌学雑誌, 71, 1, 159, 159, 2016年02月
日本細菌学会, 英語 - 東アジアの小型哺乳動物から分離されたレプトスピラの反復配列多型解析法による分子型別
小泉信夫; 泉谷秀昌; 慕蓉蓉; 岡野祥; 中島千絵; 鈴木定彦; 谷川力; 小松謙之; 吉松組子; 武藤(水谷)麻紀; 武藤(水谷)麻紀; 大西真, レプトスピラ・シンポジウム, 53rd, 5, 2016年
日本語 - CAMERA法による野外サンプル(鶏肉及び鶏糞便)からの薬剤耐性カンピロバクターの迅速検出法
臼井優; 中島千絵; 舘野翔; 田勢準也; 小野崎正修; 大曾根司朗; 鈴木定彦; 田村豊, 日本獣医学会学術集会講演要旨集, 158th, 363, 2015年08月30日
日本語 - 結核 : 終わらない脅威とその対策 (特集 新興・再興感染症と感染症対策) -- (細菌)
鈴木 定彦; 中島 千絵, 生体の科学, 66, 4, 335, 338, 2015年07月
金原一郎記念医学医療振興財団 ; 1949-, 日本語 - らい菌のDNAジャイレースに対するキノロン系抗菌薬の阻害活性試験とその結果から考える結合様式
山口智之; 中島千絵; 鈴木定彦, Jpn J Lepr, 84, 1, 26, 2015年05月25日
日本語 - Inquisition of DNA gyrase genes on fluoroquinolone resistant clinical Mycobacterium avium isolates
MENDA Conscillioah; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 191, 2015年02月25日
英語 - Detection of loop-mediated isothermal amplification reaction by non-fluorescent leuco dyes
THAPA Jeewan; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 200, 2015年02月25日
英語 - Extended-spectrum beta-lactamases in Escherichia coli isolated from swine in Thailand
CHANGKAEW Kanjana; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 190, 2015年02月25日
英語 - Diversity of MIRU-VNTR among Mycobacterium tuberculosis Central Asian family isolates from Nepal
SHAH Yogendra; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 208, 2015年02月25日
英語 - Virulence factors and class 1 integrons in Vibrio parahaemolyticus from shrimp farming environment
CHANGKWANYEUN Ruchirada; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 190, 2015年02月25日
英語 - The selection of ciprofloxacin resistance in Salmonella Typhimurium exposed residue level in vitro
KONGSOI Siriporn; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 190, 2015年02月25日
英語 - Alteration opf Mycobacterium leprae DNA gyrase activity by mutations conferring quinolone resistance
YAMAGUCHI Tomoyuki; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 191, 2015年02月25日
英語 - Understanding the circulation of Leptospira among water buffaloes in an intensive farm setting
VILLANUEVA Marvin; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 70, 1, 208, 2015年02月25日
英語 - 抗酸菌におけるパラアミノサリチル酸に対する新たな耐性機序の可能性
ZHAO Na; 中山真彰; 関塚剛史; 黒田誠; 本田尚子; 阿戸学; 中島千絵; 鈴木定彦; 大原直也, 日本細菌学雑誌, 70, 1, 193, 2015年02月25日
日本語 - ハンセン病の予防法及び診断・治療法の開発・普及に関する研究 薬剤耐性獲得機構解明とその迅速感受性試験法開発への応用
鈴木定彦; 中島千絵, ハンセン病の予防法及び診断・治療法の開発・普及に関する研究 平成26年度 総括・分担研究報告書, 13, 17, 2015年
日本語 - らい菌にキノロン系抗菌薬耐性を与える酵素内アミノ酸置換がもたらすDNAジャイレース活性低下の解析
山口智之; 中島千絵; 鈴木定彦, Jpn J Lepr, 83, 2, 47, 2014年07月25日
日本語 - Mycobacterium tuberculosis complex genotypic lineages circulating in Egyptian population
DIAB Hassan; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 69, 1, 144, 2014年02月25日
英語 - High levels of OPN, IP-10 and neutrophilia in LAMP confirmed TB patients in Manila
SHIRATORI Beata; SHIRATORI Beata; LEANO Susan; NAKAJIMA Chie; CHAGAN‐YASUTAN Haorile; NIKI Toshiro; SUZUKI Yasuhiko; TELAN Elisabeth; HATTORI Toshio, 日本細菌学雑誌, 69, 1, 140, 2014年02月25日
英語 - Tuberculosis caused by Mycobacterium orygis in dairy cattle and captured monkeys in Bangladesh
NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 69, 1, 141, 2014年02月25日
英語 - Development of loop-mediated isothermal amplification method for detection of Legionella pneumophila
CHANGKAEW Kanjana; SUZUKI Yasuhiko; NAKAJIMA Chie, 日本細菌学雑誌, 69, 1, 201, 2014年02月25日
英語 - Isolation and characterization of Mycobacterium orygis from a deer in Nepal
THAPA Jeewan; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 69, 1, 141, 2014年02月25日
英語 - Impact of amino acid substitutions in GyrA affects quinolone resistance in Salmonella Typhimurium
KONGSOI Siriporn; SUZUKI Yasuhiko; NAKAJIMA Chie, 日本細菌学雑誌, 69, 1, 196, 2014年02月25日
英語 - Impact of mutations in DNA gyrase genes on fluoroquinolone resistance in Campylobacter jejuni
CHANGKWANYEUN Ruchirada; KIM Hyun; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 69, 1, 196, 2014年02月25日
英語 - Epidemiological study of leptospirosis among water buffalo (Bubalus bubalis) in the Philippines
VILLANUEVA Marvin; KOIZUMI Nobuo; SUZUKI Yasuhiko; NAKAJIMA Chie, 日本細菌学雑誌, 69, 1, 240, 2014年02月25日
英語 - Genotypic characterization of multidrug-resistant Mycobacterium tuberculosis isolated in Myanmar
SUZUKI Yasuhiko; NAKAJIMA Chie, 日本細菌学雑誌, 69, 1, 199, 2014年02月25日
英語 - 結核菌DNAジャイレースにおけるキノロン耐性決定領域外に見出されたアミノ酸置換のキノロン剤耐性への影響(Clarification of correlation between mutants DNA GyrA and quinolone resistance in M. tuberculosis)
金 玄; 横山 和正; 中島 千絵; 森 茂太郎; 柴山 恵吾; 鈴木 定彦, 日本細菌学雑誌, 69, 1, 199, 199, 2014年02月
日本細菌学会, 英語 - サハラ以南アフリカにおけるエイズ・結核研究ネットワークの構築に関する研究
鈴木定彦; 中島千絵, サハラ以南アフリカにおけるエイズ・結核研究ネットワーク構築に関する研究 平成25年度 総括・分担研究報告書, 6, 8, 2014年
日本語 - ハンセン病の予防法及び診断・治療法の開発・普及に関する研究 薬剤耐性獲得機構解明とその迅速感受性試験法開発への応用に関する研究
鈴木定彦; 中島千絵, ハンセン病の予防法及び診断・治療法の開発・普及に関する研究 平成25年度 総括・分担研究報告書, 15, 18, 2014年
日本語 - 結核研究からハンセン病研究へ:分子生物学的見地から
鈴木定彦; 山口智之; KIM Hyun; 横山和正; 中島千絵, Jpn J Lepr, 83, 3, 131, 137, 2014年
Japanese Leprosy Association, 日本語 - 病原体特異的等温遺伝子増幅(LAMP)法によるピロリン酸の発生量をモニターするポータブルバイオセンサーの開発
矢萩洸貴; 中島千絵; 黒岩崇; 杉山龍男; 鈴木定彦; 宗像文男, 電気学会ケミカルセンサ研究会資料, CHS-13, 1-15, 71, 74, 2013年08月08日
日本語 - Applicability of In-House Loop-Mediated Isothermal Amplification for Rapid Identification of Mycobacterium tuberculosis Complex Grown on Solid Media
Benjawan Phetsuksiri; Janisara Rudeeaneksin; Sopa Srisungngam; Supranee Bunchoo; Dhanida Roienthong; Tetsu Mukai; Chie Nakajima; Shigeyuki Hamada; Yasuhiko Suzuki, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 66, 3, 249, 251, 2013年05月
英語 - 結核菌DNAジャイレース上の菌系統特異的アミノ酸多型のキノロン剤耐性への影響
金玄; 横山和正; 中島千絵; 鈴木定彦, Jpn J Lepr, 82, 1/2, 22, 22, 2013年04月01日
日本語 - 結核菌DNAジャイレースにおけるキノロン耐性決定領域外に見出されたアミノ酸置換のキノロン剤耐性への影響
金玄; 横山和正; 中島千絵; 鈴木定彦, Jpn J Lepr, 82, 1/2, 24, 24, 2013年04月01日
日本語 - Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis from Asian Countries
NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 68, 1, 217, 2013年02月25日
英語 - Inhibition activity of quinolones against DNA gyrase of Campylobacter jejuni
CHANGKWANYEUN Ruchirada; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 68, 1, 191, 2013年02月25日
英語 - Amino acid substitutions in DNA gyrase B subunit confer Quinolone resistance on Mycobacterium leprae
SUZUKI Yasuhiko; KIM Hyun; MUKAI Tetsu; NAKAJIMA Chie, 日本細菌学雑誌, 68, 1, 191, 2013年02月25日
英語 - Antimicrobial Resistance and Class 1 Integrons in Escherichia coli Isolated from Shrimp Environment
CHANGKAEW Kanjana; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 68, 1, 195, 2013年02月25日
英語 - Salmonella Typhimurium DNA Gyrase and Its Interaction with Quinolones
KONGSOI Siriporn; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 68, 1, 190, 2013年02月25日
英語 - ハンセン病の予防法及び診断・治療法の開発・普及に関する研究 薬剤耐性獲得機構解明とその迅速感受性試験法開発への応用
鈴木定彦; 松岡正典; 中島千絵, ハンセン病の予防法及び診断・治療法の開発・普及に関する研究 平成24年度 総括・分担研究報告書, 15, 20, 2013年
日本語 - Simple multiplex PCR assay for identification of Beijing family Mycobacterium tuberculosis isolates with a lineage-specific mutation in Rv0679c
Chie Nakajima; Aki Tamaru; Zeaur Rahim; Ajay Poudel; Bhagwan Maharjan; Khin Saw Aye; Hong Ling; Toshio Hattori; Tomotada Iwamoto; Yukari Fukushima; Haruka Suzuki; Yasuhiko Suzuki; Takashi Matsuba, Journal of Clinical Microbiology, 51, 7, 2025, 2032, 2013年
American Society for Microbiology, 英語 - 結核の革新的な診断・治療及び対策の強化に関する研究 多様な研究シーズを想定した結核菌臨床分離株のゲノム情報集積
和田崇之; 鈴木定彦; 中島千絵, 結核の革新的な診断・治療及び対策の強化に関する研究 平成24年度 総括・研究分担報告書, 106, 111, 2013年
日本語 - ヒトMAC症由来Mycobacterium avium subsp.hominissuisの遺伝的多様性とゲノム構造解析
岩本朋忠; 中西典子; 中島千絵; 有川健太郎; 鈴木定彦, 日本ゲノム微生物学会年会要旨集, 7th, 51, 2013年
日本語 - サハラ以南アフリカにおけるエイズ・結核研究ネットワークの構築に関する研究
鈴木定彦; 中島千絵, サハラ以南アフリカにおけるエイズ・結核研究ネットワーク構築に関する研究 平成24年度 総括・分担研究報告書, 15, 18, 2013年
日本語 - 国際共同基盤研究に応用する抗酸菌感染症研究の整備 薬剤耐性結核菌の迅速検出法
鈴木定彦; 中島千絵, 国際共同基盤研究に応用する抗酸菌感染症研究の整備 平成24年度 総括・分担研究報告書, 17, 22, 2013年
日本語 - ウシの免疫抑制受容体PD‐1に対するモノクローナル抗体の作製および免疫活性化能の検討
池渕良洋; 今内覚; 岡川朋弘; 横山和正; 中島千絵; 鈴木定彦; 村田史郎; 大橋和彦, 日本獣医学会学術集会講演要旨集, 154th, 233, 2012年08月31日
日本語 - Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification
Janisara Rudeeaneksin; Supranee Bunchoo; Sopa Srisungngam; Pathom Sawanpanyalert; Sawet Chamnangrom; Atipa Kamolwat; Porntip Thanasripakdeekul; Tooru Taniguchi; Chie Nakajima; Yasuhiko Suzuki; Benjawan Phetsuksiri, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 65, 4, 306, 311, 2012年07月
英語 - Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal
Ajay Poudel; Chie Nakajima; Yukari Fukushima; Haruka Suzuki; Basu Dev Pandey; Bhagwan Maharjan; Yasuhiko Suzuki, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56, 6, 2831, 2836, 2012年06月
英語 - Quinolone-Induced Upregulation of Osteopontin Gene Promoter Activity in Human Lung Epithelial Cell Line A549
Beata Shiratori; Jing Zhang; Osamu Usami; Haorile Chagan-Yasutan; Yasuhiko Suzuki; Chie Nakajima; Toshimitsu Uede; Toshio Hattori, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56, 6, 2868, 2872, 2012年06月
英語 - A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine
Nobuo Koizumi; Chie Nakajima; Tsunehito Harunari; Tsutomu Tanikawa; Toshihiro Tokiwa; Eriko Uchimura; Tokujiro Furuya; Claro Niegos Mingala; Marvin Ardeza Villanueva; Makoto Ohnishi; Yasuhiko Suzuki, JOURNAL OF CLINICAL MICROBIOLOGY, 50, 6, 2072, 2074, 2012年06月
英語 - A Rapid Loop-Mediated Isothermal Amplification Assay Targeting hspX for the Detection of Mycobacterium tuberculosis Complex
Aixiao Bi; Chie Nakajima; Yukari Fukushima; Aki Tamaru; Isamu Sugawara; Akio Kimura; Ryuji Kawahara; Zhongyi Hu; Yasuhiko Suzuki, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 65, 3, 247, 251, 2012年05月
英語 - Distinct Clinical Features in Nontuberculous Mycobacterial Disease with or without Latent Tuberculosis Infection
Umme Ruman Siddiqi; Haorile Chagan-Yasutan; Chie Nakajima; Hiroki Saitoh; Yugo Ashino; Osamu Usami; Beata Shiratori; Motoki Usuzawa; Yasuhiko Suzuki; Toshio Hattori, TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 226, 4, 313, 319, 2012年04月
英語 - らい菌のDNAジャイレースBサブユニット上のアミノ酸置換とニューキノロン耐性
横山和正; 金玄; 中島千絵; 松岡正典; 向井徹; 鈴木定彦, Jpn J Lepr, 81, 1/2, 48, 2012年04月01日
日本語 - らい菌のDNAジャイレースAサブユニット上のアミノ酸置換とニューキノロン耐性
横山和正; 金玄; 中島千絵; 松岡正典; 向井徹; 鈴木定彦, Jpn J Lepr, 81, 1/2, 47, 2012年04月01日
日本語 - A New Loop-Mediated Isothermal Amplification Method for Detection of Leptospira spp. in Urine
KOIZUMI Nobuo; OHNISHI Makoto; NAKAJIMA Chie; SUZUKI Yasuhiko, 日本細菌学雑誌, 67, 1, 126, 2012年02月25日
英語 - Amino Acid Substitutions at Position 95 in GyrA Can Add Fluoroquinolone Resistance to Mycobacterium leprae
Kazumasa Yokoyama; Hyun Kim; Tetsu Mukai; Masanori Matsuoka; Chie Nakajima; Yasuhiko Suzuki, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56, 2, 697, 702, 2012年02月
英語 - Influence of Lineage-Specific Amino Acid Dimorphisms in GyrA on Mycobacterium tuberculosis Resistance to Fluoroquinolones
Hyun Kim; Chie Nakajima; Youn Uck Kim; Kazumasa Yokoyama; Yasuhiko Suzuki, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 65, 1, 72, 74, 2012年01月
英語 - Loop‐mediated isothermal amplification法による尿からの簡便なレプトスピラ検出法
小泉信夫; 中島千絵; 春成常仁; 谷川力; 常盤俊大; 内村江利子; 古谷徳次郎; MINGALA Claro Niegos; VILLANUEVA Marvin Ardeza; 大西真; 鈴木定彦, レプトスピラ・シンポジウム, 49th, 7, 2012年
日本語 - 海外から輸入される多剤耐性結核に関する研究 黒竜江省で収集した結核菌の分子疫学的解析
服部俊夫; 凌虹; 鈴木定彦; 中島千絵, 海外から輸入される多剤耐性結核に関する研究 平成23年度 総括・分担研究報告書, 88, 90, 2012年
日本語 - サハラ以南アフリカにおけるエイズ・結核研究ネットワークの構築に関する研究 ザンビアにおける結核菌の分子疫学的解析
鈴木定彦; 中島千絵, サハラ以南アフリカにおけるエイズ・結核研究ネットワーク構築に関する研究 平成23年度 総括・分担研究報告書, 6, 8, 2012年
日本語 - CORRELATION BETWEEN BEIJING GENOTYPE FAMILY AND THE ENVELOPE PROTEIN IN MYCOBACTERIUM TUBERCULOSIS
MATSUBA Takashi; NAKAJIMA Chie; TAMARU Aki; SIDDIQI Umme R; HATTORI Toshio; RAHIM Zeaur; POUDEL Ajay; PANDEY Basu D; PANDEY Basu D; SUZUKI Yasuhiko, 日本細菌学雑誌, 66, 2-3, 426, 2011年10月31日
英語 - Impact of the E540V Amino Acid Substitution in GyrB of Mycobacterium tuberculosis on Quinolone Resistance
Hyun Kim; Chie Nakajima; Kazumasa Yokoyama; Zeaur Rahim; Youn Uck Kim; Hiroki Oguri; Yasuhiko Suzuki, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 55, 8, 3661, 3667, 2011年08月
英語 - Genotypes and Characteristics of Clustering and Drug Susceptibility of Mycobacterium tuberculosis Isolates Collected in Heilongjiang Province, China
Juan Wang; Yan Liu; Chun-Lei Zhang; Bin-Ying Ji; Liu-Zhuo Zhang; Yong-Zhen Shao; Shui-Lian Jiang; Yasuhiko Suzuki; Chie Nakajima; Chang-Long Fan; Yuan-Ping Ma; Geng-Wen Tian; Toshio Hattori; Hong Ling, JOURNAL OF CLINICAL MICROBIOLOGY, 49, 4, 1354, 1362, 2011年04月
英語 - オホーツクと南極海で採取した流氷における重金属濃度と金属耐性遺伝子の検出
能田淳; 登坂唯香; 中島千絵; 大久保寅彦; 石原加奈子; 鈴木定彦; 伊村智; 田村豊, 日本獣医学会学術集会講演要旨集, 151st, 248, 2011年03月01日
日本語 - 国際共同基盤研究に応用する抗酸菌感染症研究の整備 薬剤耐性結核菌の迅速検出法
鈴木定彦; 中島千絵, 国際共同基盤研究に応用する抗酸菌感染症研究の整備 平成22年度 総括・分担研究報告書, 21-25, 2011年
日本語 - らい菌および結核菌のニューキノロン耐性獲得機構と耐性菌迅速鑑別法
金 玄; 鈴木 晴香; 松岡 正典; 松葉 隆司; 横山 和正; 中島 千絵; 鈴木 定彦, 日本ハンセン病学会雑誌 = Japanese journal of leprosy, 80, 1, 17, 27, 2011年
日本ハンセン病学会, 英語 - 結核菌のエンベロープ構造と成分
松葉 隆司; 中島 千絵; 鈴木 定彦, 日本細菌学雑誌, 65, 3, 355, 368, 2010年12月24日
細菌は属や種による莢膜やべん毛の有無などを含む詳細な構造の違いはあるが，菌体表層を覆うエンベロープ構造の違いに由来する染色性を指標に，グラム陰性菌と陽性菌に大別されてきた。両者の構造上の大きな違いは，グラム陰性菌には外膜と内膜（細胞質膜），ペリプラズム間隙およびリポ多糖体（LPS）があり，グラム陽性菌には外膜，ペリプラズム間隙やLPSがなくペプチドグリカン量が多いこととされている。しかしながら，グラム染色性により分類しにくい結核菌を含むマイコバクテリウム属細菌については，グラム陰性菌と類似したエンベロープ構造を有する可能性が長年指摘されてきた。近年，電子顕微鏡装置や試料作製技術の進歩と化学構造解析研究の発展に伴い，より詳細な結核菌のエンベロープ構造が明らかとされている。一方で，分子生物学的手法を用いて結核菌エンベロープ各成分の生合成経路，機能や免疫生物活性に関する知見も積み重ねられてきている。本稿では，結核菌エンベロープ構造や成分について最近の知見を中心に，著者らの成績も含めて概説したい。, 日本細菌学会, 日本語 - Possible Mode of Emergence for Drug-Resistant Leprosy Is Revealed by an Analysis of Samples from Mexico
Masanori Matsuoka; Yasuhiko Suzuki; Iris Estrada Garcia; Mary Fafutis-Morris; Alberto Vargas-Gonzalez; Cristina Carreno-Martinez; Yukari Fukushima; Chie Nakajima, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 63, 6, 412, 416, 2010年11月
英語 - Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital
Kanako Ishihara; Natsumi Shimokubo; Akie Sakagami; Hiroshi Ueno; Yasukazu Muramatsu; Tsuyoshi Kadosawa; Chie Yanagisawa; Hideaki Hanaki; Chie Nakajima; Yasuhiko Suzuki; Yutaka Tamura, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76, 15, 5165, 5174, 2010年08月
英語 - A Novel Method for the Purification of DNA by Capturing Nucleic Acid and Magnesium Complexes on Non-Woven Fabric Filters under Alkaline Conditions for the Gene Diagnosis of Tuberculosis by Loop-Mediated Isothermal Amplification (LAMP)
Tadashi Fukasawa; Naozumi Oda; Yasunao Wada; Aki Tamaru; Yukari Fukushima; Chie Nakajima; Yasuhiko Suzuki, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 63, 4, 246, 250, 2010年07月
英語 - 南極およびオホーツク海で採取した流氷における構成菌種の同定と薬剤耐性遺伝子の検出
登坂唯香; 中島千絵; 大久保寅彦; 石原加奈子; 鈴木定彦; 伊村智; 田村豊, 極域科学・宙空圏・気水圏・生物・地学シンポジウム講演予稿集(CD−ROM), 2010, ROMBUNNO.PB23, 2010年
日本語 - 結核と非結核性抗酸菌症診療の新展開 6.超多剤耐性結核菌における耐性化のメカニズムと耐性化防止対策
鈴木定彦; 中島千絵; 福島由華里; 田丸亜貴; 松葉隆司, 化学療法の領域, 25, 4, 612-620, 2009年03月25日
日本語 - 特徴ある感染症対策—Post‐exposure Prophylaxis:PEPを中心に 曝露後発症対策(Post‐exposure Prophylaxis:PEP)結核(化学予防)
鈴木定彦; 中島千絵; 福島由華里; 田丸亜貴; 松葉隆司, 臨床と微生物, 35, 6, 669-675, 2008年11月25日
日本語 - 人及び豚の抗酸菌感染症に由来するMycobacterium aviumの遺伝学的特長の比較
中島千絵; 田村豊; 田丸亜貴; 鈴木定彦, 感染症学雑誌, 82, 6, 767, 2008年11月20日
日本語 - Effects of anti-tick cocktail vaccine against Rhipicephalus appendiculatus
Saiki Imamura; Satoru Konnai; Itabajara da Silva Vaz Junior; Shinji Yamada; Chie Nakajima; Yuko Ito; Tomoko Tajima; Jun Yasuda; Martin Simuunza; Misao Onuma; Kazuhiko Ohashi, JAPANESE JOURNAL OF VETERINARY RESEARCH, 56, 2, 85, 98, 2008年08月
英語 - 結核の化学予防
鈴木定彦; 中島千絵; 福島由華里; 田丸亜貴; 松葉隆司, 臨床と微生物, 25, 669, 675, 2008年 - rsmG変異によるストレプトマイシン耐性機構の解明および臨床分離結核菌における本変異の重要性
岡本晋; 田丸亜貴; 中島千絵; 西村賢治; 西村賢治; 田中幸徳; 田中幸徳; 徳山真治; 鈴木定彦; 越智幸三, 日本放線菌学会大会講演要旨集, 22nd, 45, 2007年05月31日
日本語 - 抗生物質耐性菌のその獲得メカニズムを解明
越智幸三; 岡本晋; 西村賢治; 西村賢治; 鈴木定彦; 田丸亜貴; 中島千絵; 田中幸徳; 田中幸徳; 徳山真治, 食品試験研究成果情報, 19, 2007年 - 多剤耐性結核の現状と今後 6.多剤耐性結核の菌検出法の進歩
鈴木定彦; 田丸亜貴; 中島千絵; 西原みづき; 福島由華里; 松葉隆司, 化学療法の領域, 22, 11, 1705-1713, 2006年10月25日
日本語 - フタトゲチマダニ (Haemaphysalis longicornis) の唾液腺由来cDNAライブラリーより得られた新規のトロンビン阻害蛋白質をコードする遺伝子
中島 千絵; 今村 彩貴; 今内 覚; 山田 慎二; 西門 秀人; 大橋 和彦; 小沼 操, The journal of veterinary medical science, 68, 5, 447, 452, 2006年05月25日
吸血中のフタトゲチマダニ(Haemaphysalis longicornis)の唾液腺に由来するcDNAライブラリーより,トロンビン阻害作用を持つ蛋白質をコードする遺伝子を同定した.この蛋白質(chimadaninと命名)は93アミノ酸残基より成り,分泌シグナルを持つことから分泌蛋白質であることが推測された.相同性検索において既知の蛋白質あるいは保存ドメイン配列データベースより類似のものは検出されず,新規な蛋白質配列であると判断された.また,ダニの吸血段階及び臓器別に遺伝子の発現を調べたところ,吸血開始後の主として唾液腺において発現することが示唆された.推定成熟ペプチド部の塩基配列を大腸菌に組み込んで発現・精製し,リコンビナント蛋白質の機能を調べたところ,血液凝固系の検査である活性化部分トロンボプラスチン時間及びプロトロンビン時間の両試験において,濃度依存的に明らかな凝固時間延長作用を示した.また,トロンビンによるペプチド基質の分解を濃度依存的に抑制したため,トロンビンの酵素活性阻害作用を持った血液凝固抑制蛋白質であることが示唆された.この蛋白質は,ダニの吸血において重要な働きをしている可能性があると考えられる., 社団法人日本獣医学会, 英語 - A novel gene encoding a thrombin inhibitory protein in a cDNA library from Haemaphysalis longicornis salivary gland
C Nakajima; S Imamura; S Konnai; S Yamada; H Nishikado; K Ohashi; M Onuma, JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, 5, 447, 452, 2006年05月
英語 - 吸血中のフタトゲチマダニ (Haemaphysalis longicornis) 唾液腺由来cDNAライブラリーによる発現遺伝子の網羅的解析
中島 千絵; da Silva Vaz Jr. Itabajara; 今村 彩貴; 今内 覚; 大橋 和彦; 小沼 操, The journal of veterinary medical science, 67, 11, 1127, 1131, 2005年11月25日
吸血中のフタトゲチマダニ(Haemaphysalis longicornis)より唾液腺を採取してcDNAライブラリーを作成し, ランダムに選んだ発現タンパク遺伝子の配列をblastx (NCBI)等のプログラムを用いて解析した.得られた633配列はその相同性によって, 同一あるいはごく近縁の遺伝子由来と思われる213配列に集約された.全633配列のうち, blastxを用いた相同性検索により発現タンパクの機能予測がなされたものは全体の36%であり, 残りの64%は未知の遺伝子であった.機能が予測されたタンパクの大半は細胞の生存に必要なハウスキーピングタンパクであったが, 血液凝固阻害因子類似の蛋白を含めたプロテアーゼインヒビターやメタロプロテアーゼ, 他のダニで分離されている免疫抑制物質に類似したタンパク遺伝子等も同定された.これらのタンパクはダニの吸血に際し何らかの重要な役割を演じている可能性があり, 新たな抗ダニワクチン抗原の候補となりうると考えられる., 社団法人日本獣医学会, 英語 - Random sequencing of cDNA library derived from partially-fed adult female Haemaphysalis longicornis salivary gland
C Nakajima; ID Vaz; S Imamura; S Konnai; K Ohashi; M Onuma, JOURNAL OF VETERINARY MEDICAL SCIENCE, 67, 11, 1127, 1131, 2005年11月
英語 - コイタマダニ由来セルピンの抗ダニカクテルワクチン効果
今村彩貴; BONIFACE Mamangala; 今内覚; 中島千絵; MWASE Tembo; 田島朋子; 安田準; 大橋和彦; 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 84, 2005年08月31日
日本語 - フタトゲチマダニ由来新規メタロプロテアーゼ遺伝子のクローニングと性状解析
今村彩貴; 今内覚; 中島千絵; 大橋和彦; 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 78, 2005年08月31日
日本語 - ザンビア共和国における牛およびRhipicephalus appendiculatusからのTheileria parvaの検出
今内覚; 今村彩貴; 中島千絵; SIMUNZA Martin; AMOS Chota; CHEMBENSOFU Mwelwa; ANDOREU Nambota; 安田準; 大橋和彦; 小沼操, 日本獣医学会学術集会講演要旨集, 140th, 84, 2005年08月31日
日本語 - ワクチン研究の新展開—多様な手法が切り開くワクチンの可能性—ダニの吸血を阻害する抗ダニワクチンの開発
小沼操; 今村彩貴; 中島千絵, 獣医畜産新報, 998, 753-757, 2004年09月01日
日本語 - ダニの吸血を阻害する抗ダニワクチンの開発 (特集:ワクチン研究の新展開--多様な手法が切り開くワクチンの可能性 第137回日本獣医学会学術集会 2004年4月2日 日本大学)
小沼 操; 今村 彩貴; 中島 千絵, 獣医畜産新報, 57, 9, 753, 757, 2004年09月
文永堂出版, 日本語 - アメリカ腐蛆病菌芽胞に対する各種消毒剤の殺菌効果
岡山 敦子; 追川 朋子; 中島 千絵; 吐山 豊秋, The journal of veterinary medical science, 59, 10, 953, 954, 1997年10月25日
社団法人日本獣医学会, 英語 - 蜜蜂及びその巣箱におけるアンピシリンの動態
中島 千絵; 岡山 敦子; 迫川 朋子; 中村 晃; 吐山 豊秋, The journal of veterinary medical science, 59, 9, 765, 767, 1997年09月25日
社団法人日本獣医学会, 英語 - アメリカ腐蛆病の防除に有効な薬剤の検索
吐山 豊秋; 岡山 敦子; 中島 千絵; 迫川 朋子; 片岡 重明; 中村 晃, 日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association, 50, 8, 429, 437, 1997年08月20日
日本獸医師会, 日本語 - アメリカ腐しゃ病の防除に有効な薬剤の検索
吐山豊秋; 岡山敦子; 中島千絵; 迫川朋子; 片岡重明; 中村晃, 日本獣医師会雑誌, 50, 8, 429-437, 437, 1997年08月
公益社団法人 日本獣医師会, 日本語 - アメリカ腐しょ病菌をin vitro感染した蜜蜂幼虫に対するミロサマイシンの効果
迫川朋子; 片岡重明; 岡山敦子; 中島千絵; 吐山豊秋, 日本獣医学会学術集会講演要旨集, 123rd, 169, 1997年03月
日本語 - HPLCによる鶏・豚組織中のスペクチノマイシンの残留分析法
大口 克志; 高原 ひろみ; 松本 真里子; 中島 千絵; 城戸 靖雅, 食品衛生学雑誌, 37, 5, 319, 324, 1996年10月05日
日本語 - アメリカ腐蛆病菌Bacillus larvae日本分離株の生物性状と薬剤感受性
岡山 敦子; 迫川 朋子; 中島 千絵; 吐山 豊秋, The journal of veterinary medical science, 58, 5, 439, 441, 1996年05月25日
英語 - 第17巻2号-02 閉鎖系飼育室内のフライトルームにおけるミツバチ飼育管理
中村 晃; 杉本 忠美; 岡山 敦子; 迫川 朋子; 中島 千絵, ミツバチ科学, 17, 2, 61, 66, 1996年04月20日
日本語 - フェンプロスタレンによる豚の分娩誘起
伊東正吾; 曽根勝; 鈴木邦夫; 和泉屋公一; 望月洋; 筒井敏彦; 中島千絵; 小笠晃; 中原達夫, Anim Sci Technol, 65, 9, 834-841, 841, 1994年09月
妊娠豚延べ273頭を用い,半減期が著しく長いProstalandin F2α誘導体,フェンプロスタレン(FP)の分娩誘起効果を臨床•内分泌学的に検討した.FPの投与時期は,妊娠111,112,113,114日(最終交配日を0日)の4区,また投与量は0.25,0.5,1.Omgおよび対照(生理食塩液)の4水準とし,頸部に皮下注射した.処置から分娩開始までの時間は,対照区と比較して,0.25mg投与区では有意差が見られなかったが,0.5および1.Omg投与区では有意(p<0.01)に短かった.また,0.5mgおよび1.0mgを妊娠111～113日に投与した試験区では,いずれも処置後24時間前後,114日に投与した試験区では処置後12時間前後に分娩が誘起される傾向が認められた.なお,分娩誘起時の産子数,死産子数,子豚の生時および離乳時体重,さらに離乳後における母豚の発情回帰については対照区との間に有意差は認められなかった.FPの血中移行は,その代謝産物(RS-63377)を指標にしてみると比較的速やかで,代謝産物の血中濃度は1時間で増加し始め,2～4時間前後に最高値を示して減少するが,投与後24時間以降も比較的高濃度を維持していた.また,FP投与後血中プロジェスラロン濃度は速やかに低下し,その後分娩が開始されることを認めた.以上のように,FPO.5～1.Omg投与は妊娠111～114日の豚について顕著な分娩誘起作用を発揮することが示された., Japanese Society of Animal Science, 日本語 - 薬物毒性 A‐81(flunixin meglumine製剤)錠剤のイヌにおける安全性
福田苗美; 小田憲司; 西田由美; 芳賀知也子; 中島千絵; 長沢朋子; 氏政雄揮; 片江宏巳; 吐山豊秋, 日本獣医学会学術集会講演要旨集, 118th, 73, 1994年08月
日本語 - A‐81(Flunixin meglumine製剤)注射剤のイヌにおける安全性
小田憲司; 福田苗美; 西田由美; 芳賀知也子; 中島千絵; 長沢朋子; 氏政雄揮; 片江宏巳; 吐山豊秋, 日本獣医学会学術集会講演要旨集, 117th, 87, 1994年03月
日本語 - フェンプロスタレンによる豚の分娩誘起
伊東 正吾; 曽根 勝; 鈴木 邦夫; 和泉屋 公一; 望月 洋; 筒井 敏彦; 中島 千絵; 小笠 晃; 中原 達夫, 日本畜産学会報, 65, 9, 834, 841, 1994年
妊娠豚延べ273頭を用い,半減期が著しく長いProstalandin F2α誘導体,フェンプロスタレン(FP)の分娩誘起効果を臨床•内分泌学的に検討した.FPの投与時期は,妊娠111,112,113,114日(最終交配日を0日)の4区,また投与量は0.25,0.5,1.Omgおよび対照(生理食塩液)の4水準とし,頸部に皮下注射した.処置から分娩開始までの時間は,対照区と比較して,0.25mg投与区では有意差が見られなかったが,0.5および1.Omg投与区では有意(p<0.01)に短かった.また,0.5mgおよび1.0mgを妊娠111～113日に投与した試験区では,いずれも処置後24時間前後,114日に投与した試験区では処置後12時間前後に分娩が誘起される傾向が認められた.なお,分娩誘起時の産子数,死産子数,子豚の生時および離乳時体重,さらに離乳後における母豚の発情回帰については対照区との間に有意差は認められなかった.FPの血中移行は,その代謝産物(RS-63377)を指標にしてみると比較的速やかで,代謝産物の血中濃度は1時間で増加し始め,2～4時間前後に最高値を示して減少するが,投与後24時間以降も比較的高濃度を維持していた.また,FP投与後血中プロジェスラロン濃度は速やかに低下し,その後分娩が開始されることを認めた.以上のように,FPO.5～1.Omg投与は妊娠111～114日の豚について顕著な分娩誘起作用を発揮することが示された., 公益社団法人 日本畜産学会, 日本語 - 鶏肉中Spectinomycinの残留分析法の開発に関する研究
高原ひろみ; 岡田真里子; 大口克志; 中島千絵; 城戸靖雅, 日本食品衛生学会学術講演会講演要旨集, 65th, 63, 1993年
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2021年04月01日 - 2024年03月31日
佐藤 豊孝; 小笠原 徳子; 山本 聡; 浦野 恵美子; 青木 弘太郎; 中島 千絵; 臼井 優; 鈴木 仁人; 大久保 寅彦; 福田 昭
本研究では、フルオロキノロン耐性大腸菌ST131をモデルに、『院内』で問題となるAMR感染症を『院外(市中)』からのプローチでその伝播・定着様式を明らかにすることで、現行のAMR対策が報われない国際的なAMR問題の根本的解決に繋がる次世代型(院内-市中一体型)AMR対策に資する科学的知見を提供することが本研究の目的である。本目的達成の為、本年度は、ST131の伝播状況と伝播様式および定着メカニズム(定着因子の同定)を行なった。具体的には、同一地域（札幌市内）から同一時期に院内及び市中の各サンプル群(患者、健常者、動物、環境)から大腸菌を一斉に分離した。その後、シプロフロキサシンに耐性だった株を対象にST131の検出PCRおよび次世代シーケンス解析により行なった。その結果、ヒトの臨床検体(大学病院)からは25%, ヒトの臨床検体(市中クリニック)からは19.2%, ヒトの健常者の便検体からは16.1%, 伴侶動物(犬や猫)の直腸スワブからは18.3%, 家畜の牛、豚、鶏の便検体からはそれぞれ9.5%, 0%, 6.7%のフルオロキノロン耐性率であった。分離大腸菌全体に占めるST131の割合は、ヒトの臨床検体(大学病院)からは12.5%, ヒトの臨床検体(市中クリニック)からは5.7%, ヒトの健常者の便検体からは8.2%, 伴侶動物(犬や猫)の直腸スワブからは15.9%, 家畜の牛、豚、鶏の便検体からは1株も分離されなかった。
日本学術振興会, 基盤研究(B), 札幌医科大学, 21H03622 - アジア・オセアニアにおける病原性抗酸菌の浸淫度調査
科学研究費助成事業 基盤研究(B)
2015年04月01日 - 2019年03月31日
和田 崇之; 柳井 徳磨; 吉田 志緒美; 鈴木 定彦; 中島 千絵; 斑目 広郎; 今城 雅之
抗酸菌属は多様な自然環境に生存する一方，病原菌としての側面を持ち，ヒトを含めた様々な動物に感染して病変を起こす．本課題では，国内外における野生動物・捕獲動物に関して抗酸菌症の調査研究を行い，原因菌のゲノム解読に基づく遺伝子解析を行った．国際連携としては，台湾との連携の下，新規Mycobacterium marinum検出法を確立し，飼育動物例における菌種同定を行った．飼育動物，家畜動物，伴侶動物の症例において，それぞれ異なる抗酸菌種の分離培養と比較ゲノム解析を行い，個々の菌種および分離株における遺伝的多様性について，新たな知見を蓄積することに成功した．
日本学術振興会, 基盤研究(B), 長崎大学, 15H05263 - 結核菌が多剤耐性、超多剤耐性化するメカニズムの解明とそのマーカーの探索
科学研究費助成事業 基盤研究(C)
2015年04月01日 - 2018年03月31日
中島 千絵; 鈴木 定彦
結核菌の多剤耐性化は、薬剤の作用分子をコードする遺伝子上に順次変異が蓄積されて行くことによって生じるが、特定の菌株でより耐性化や耐性菌による二次感染が起こりやすいことが知られている。結核高蔓延国であるミャンマー、ネパール、タイにおいて得られた多剤耐性結核菌株の遺伝子解析を行ったところ、北京型のモダンタイプと呼ばれる系統の割合が薬剤耐性の度合いと共に上昇し、また、イソニアジド耐性に係るKatG315の変異等、特定の遺伝子変異を持つ株が蔓延している傾向にあった。これらの特徴は感染性の高い多剤耐性結核菌を早期に特定するためのマーカーとして使用可能であると考えられ、簡易検出用のPCR法の開発に至った。
日本学術振興会, 基盤研究(C), 北海道大学, 15K08724 - アジア地域での多剤および超多剤耐性結核の伝播動態調査
科学研究費助成事業 基盤研究(B)
2012年04月01日 - 2016年03月31日
鈴木 定彦; 中島 千絵
多剤、並びに超多剤耐性結核の調査が不十分と考えられるネパール、バングラデシュ、ミャンマー、タイおよびフィリピンにおいて結核あるいは結核様症状を呈したヒトより総計2,208株の抗酸菌を分離して当該国において菌株バンクとして保存した。さらに、これらの菌株からDNAを抽出して、当該国、並びに日本においてDNAバンクとして保存した。
日本に輸入したDNAを対象として遺伝子型を決定するとともに薬剤耐性関連遺伝子変異の解析を実施した結果、ネパールおよびタイにおいては、多剤、並びに超多剤耐性結核の多くがBeijing型結核菌により引き起こされ、急速な伝播動態を示している事が明らかとなった。
日本学術振興会, 基盤研究(B), 北海道大学, 24406021 - 広宿主域を持つ新たな結核菌群菌の遺伝学的解析
科学研究費助成事業 基盤研究(C)
2012年04月01日 - 2015年03月31日
中島 千絵; 鈴木 定彦
広い宿主域を持つと考えられる新たな結核菌群菌Mycobacterium orygisを、バングラデシュ及びネパールの複数の動物種の肺結核病変から分離した。その遺伝学的解析により、この飛沫感染を起こすと考えられる結核菌群菌が、これらの国々の野生動物の間で保持されている可能性が示唆された。また、ネパールにおいてゾウから分離されたヒト型結核菌の遺伝子解析を行ったところ、トランスポゾンの活性化によると考えられるゲノムの小規模な構造変化が観察された。これらの異種動物感染例から得られた菌株の遺伝学的解析により、結核菌群菌の宿主指向性に関与するゲノム上の領域が同定できる可能性がある。
日本学術振興会, 基盤研究(C), 北海道大学, 研究代表者, 競争的資金, 24580443 - 低コスト・高精度を実現する新規等温増幅法による結核菌迅速検出法の開発
産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 探索タイプ
2011年 - 2011年
中島 千絵
本研究では我々が新たに開発した等温遺伝子増幅法の臨床現場における有用性の評価を行った。本法は簡易な恒温器があれば実施が可能であり、簡単な操作で1時間以内に病原体の存在を確認することができる。結核は検査時の菌培養に長期間を要するため迅速簡便な検査法が求められている感染症であるが、本法による喀痰からの結核菌検出成績を従来法である塗抹・PCR法、培養法、LAMP法と比較したところ、これらの方法に匹敵する結果が得られ、臨床応用の可能性が示された。本法は従来法より試薬費が掛からず安価な検査法であるため、結核の蔓延が問題となっている途上国での展開にも有益であると考える。将来的には、結核に限らず熱帯地域で問題となっている各種感染症への応用を目指したい。
北海道大学 - アジア地域での多剤および超多剤耐性結核菌の浸淫度調査
科学研究費助成事業 基盤研究(B)
2009年 - 2011年
鈴木 定彦; 中島 千絵; 松葉 隆司
多剤および超多剤耐性結核の調査が不十分と考えられるアジアの国々のうち、特にミャンマー、バングラデシュならびにネパールに焦点を絞り、臨床分離結核菌株を収集し、リファンピシンならびにイソニアジド耐性と遺伝子変異の関係を調査した。ミャンマーの多剤耐性結核菌のrpoB遺伝子変異ならびkatG又はinhA遺伝子変異保有割合はそれぞれ71.3ならびに72.5%であった。一方、バングラでシュにおいてはrpoB遺伝子変異ならびkatG又はinhA遺伝子変異保有割合はそれぞれ95.0ならびに94.5%であり、ネパールにおいてはそれぞれ97.2ならびに93.8%であった。また、バングラデシュにおいて多剤耐性結核菌218株中7株、ネパールにおいては多剤耐性結核菌109株中13株の超多剤耐性結核菌を見出した。これらの超多剤耐性結核菌全てにおいてカナマイシン耐性rrsならびにgyrAまたはgyrB遺伝子上に変異が見られた。薬剤耐性と遺伝子変異の相関は国ごとに異なるものであり、遺伝子変異分析を基盤とする結核菌薬剤感受性試験を実施するにあたっては事前にその相関を調査する必要があるものと結論された。また、今回の調査により超多剤耐性結核菌の存在がバングラデシュならびにネパールおいて明らかになったことから、これらの伝播動態調査が急務と考えられた。
日本学術振興会, 基盤研究(B), 北海道大学, 連携研究者, 競争的資金, 21406020
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