研究者基本情報

• 博士 (歯学), 北海道大学, 2021年03月
• 修士 (歯学), Universiti Sains Malaysia, 2017年09月

• https://researchmap.jp/Rafiqul-Islam

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202101018250871228
• https://orcid.org/0000-0002-1409-8112

• 00906414

• https://orcid.org/0000-0002-1409-8112

• 202101018250871228

• Pulp capping materials
• Bioactive Materials
• Nanotechnology
• Pulp biology
• Dental Materials

• ライフサイエンス, 分子生物学, Human Dental Pulp Cells
• ライフサイエンス, 保存治療系歯学, Platinum Nanoparticles

• 学士課程, 歯学部
• 博士課程, 歯学院

研究活動情報

• Ultrastructural Evaluation of Newly Formed Dentine After Direct Pulp Capping With Mineral Trioxide Aggregate and Calcium Hydroxide Using Focused Ion Beam Scanning Electron Microscopy and Stimulated Emission Depletion
  Bu'er Qi; Yu Toida; Rafiqul Islam; Md Refat Readul Islam; Kenta Tsuchiya; Yuan Yuan; Hidehiko Sano; Tomoka Hasegawa; Monica Yamauti; Atsushi Tomokiyo
  International Endodontic Journal, 2026年08月
  英語, 研究論文（学術雑誌）, ABSTRACT
  
  Aim
  To investigate the ultrastructure of the mineralised tissue after direct pulp capping (DPC) treatment using focused ion beam scanning electron microscopy (FIB‐SEM) and stimulated emission depletion (STED) microscopy.
  
  
  Methodology
  Eighteen male Wistar rats (36 maxillary first molars) were used in this study. Nine rats (18 M) were allocated for histological evaluation and immunofluorescence imaging (STED), six rats (12 M) for FIB‐SEM observation, and three rats (6 M) served as untreated controls; control molars were used for both immunofluorescence imaging and FIB‐SEM. For experimental allocation, two molars from each rat were randomly assigned to one of three groups: NEX MTA (NMTA), Dycal, or no‐capping material (NC). After 4 weeks, teeth were collected for histological analysis, FIB‐SEM observation and immunofluorescence imaging. Tubulin‐b3 and nestin expression were examined by immunofluorescence staining and imaged with STED microscopy. The Kruskal–Wallis test followed by Dunn's multiple comparisons test and one‐way ANOVA followed by Tukey's multiple comparisons test were performed to assess significant differences.
  
  
  Results
  
  All experimental groups exhibited complete mineralised tissue formation near the exposure site. The NMTA group showed a significantly more regular odontoblastic layer than the Dycal and NC groups (
  p
   < 0.05). FIB‐SEM demonstrated that NMTA induced a well‐organised and continuous tubular structure, whereas Dycal and NC produced irregular, discontinuous, and poorly organised mineralised barriers. STED imaging of the NMTA group revealed strong and organised co‐localization of tubulin‐β3 and nestin resembling primary dentine, whereas the Dycal and NC groups showed weak, disorganised, or absent signals and lacked co‐localised processes.
  
  
  
  Conclusions
  NMTA showed superior performance compared with Dycal, consistently promoting well‐organised dentine bridge formation, a continuous odontoblastic layer, and coordinated neural marker‐positive processes. This indicates that NMTA more effectively supports structural formation of the pulp‐dentine complex after DPC.
  
• Behavioral Changes and Determinants of Preventive Oral-Health Practices Among Chinese Dental Patients: A Hospital-Based Cross-Sectional Study in Guangzhou
  Jiali Yu; Zhe Ji; Rui Jiang; Rafiqul Islam; Xiang Li; Ermin Nie
  Dentistry Journal, 2026年07月21日
  研究論文（学術雑誌）
• Oral Ulcers and Associated Factors in Patients with Type 2 Diabetes Mellitus: A Cross-Sectional Study in the Bangladesh Population
  Salma Sadia; Israt Jahan Trina; Wakar Mahmud; Umme Habiba; Samiul Haque; Golam Sharower; Atsushi Tomokiyo; Rafiqul Islam
  Oral, 2026年05月
  研究論文（学術雑誌）
• Bioactivity and biocompatibility of bioceramic-based pulp capping materials in laboratory and animal models
  Rafiqul Islam; Md. Refat Readul Islam; Kenta Tsuchiya; Yu Toida; Hidehiko Sano; Monica Yamauti; Hany Mohamed Aly Ahmed; Atsushi Tomokiyo
  Journal of Materials Science: Materials in Medicine, 2025年10月14日
  研究論文（学術雑誌）
• The relationship between bond strengths and hardness tests of resin cements
  Yu Toida; Md Refat Readul Islam; Kenta Tsuchiya; Rafiqul Islam; Shuhei Hoshika; Hidehiko Sano; Atsushi Tomokiyo
  International Journal of Adhesion and Adhesives, 142, 104124, 104124, Elsevier BV, 2025年10月
  研究論文（学術雑誌）
• Emerging trends of injectable hydrogels for vital pulp therapy: A comprehensive review
  Md Refat Readul Islam; Rafiqul Islam; Hidehiko Sano; Yu Toida; Shuhei Hoshika; Hany Mohamed Aly Ahmed; Atsushi Tomokiyo
  International Endodontic Journal, 2025年10月
  研究論文（学術雑誌）
• Prevention of Tooth Discoloration Using Fluoride Varnish Immediately After Bleaching
  Ryotaro Yago; Chiharu Kawamoto; Rafiqul Islam; Hirofumi Kaneko; Monica Yamauti; Masayuki Otsuki; Hidehiko Sano; Atsushi Tomokiyo
  Journal of Functional Biomaterials, 2025年07月03日
  研究論文（学術雑誌）
• Physicochemical properties and apatite precipitation behavior of experimental calcium silicate-based cements doped with phosphate compounds
  Kenta Tsuchiya; Salvatore Sauro; Hidehiko Sano; Jukka P. Matinlinna; Monica Yamauti; Shuhei Hoshika; Yu Toida; Rafiqul Islam; Atsushi Tomokiyo
  Dental Materials, 41, 9, 1058, 1066, Elsevier BV, 2025年06月
  研究論文（学術雑誌）
• Clinical applications and classification of calcium silicate-based cements based on their history and evolution: a narrative review.
  Kenta Tsuchiya; Salvatore Sauro; Hidehiko Sano; Jukka P Matinlinna; Monica Yamauti; Shuhei Hoshika; Yu Toida; Rafiqul Islam; Atsushi Tomokiyo
  Clinical oral investigations, 29, 4, 187, 187, 2025年03月17日, ［国際誌］
  英語, 研究論文（学術雑誌）, OBJECTIVES: The objective of this narrative review was to analyze the evolution of the calcium silicate-based cement (CSC) products since 1993 and classify them to better understand their appropriate use in clinical practice and foster innovation in dental material development. MATERIALS AND METHODS: A narrative review of the relevant literature was conducted, collecting findings from computerized databases (PubMed, Science Direct, SCOPUS, and Web of Science) to provide historical background, classification, and modifications of CSCs published between 1993 and 2024. RESULTS: We classified CSCs into six distinct generations based on previous literature. This classification revealed that the improvement in CSC's drawbacks, especially in physio-chemical properties, have led to the development of novel CSCs products. It also exhibited little to no variation in the bioactivity of CSCs across different generations and raised concerns regarding the biocompatibility of resin-modified CSC. CONCLUSIONS: No CSC has yet emerged as a potential gold-standard material for endodontic treatments. Therefore, clinical applications tailored to the characteristics of each generation of CSCs are essential. Future advancements are anticipated to enable CSCs to induce the regeneration of tissues such as dentin, cementum, periodontal ligament, and dental pulp by incorporating regulatory signals. CLINICAL RELEVANCE: This comprehensive classification system would assist clinicians in choosing the appropriate CSCs for various clinical situations by considering critical factors. This systematic approach enables clinicians to rely on evidence-based material selection rather than marketing claims. Furthermore, the insights into their evolution, classification, and clinical applications would fill a knowledge gap for clinicians and researchers.
• Visibility of Recurrent Caries Through Universal Shade Resin Composite Restorations.
  Ryotaro Yago; Chiharu Kawamoto; Di Wu; Takuma Mirokuin; Rafiqul Islam; Monica Yamauti; Hidehiko Sano; Atsushi Tomokiyo
  Materials (Basel, Switzerland), 17, 23, 2024年11月27日, ［国際誌］
  英語, 研究論文（学術雑誌）, This in vitro study aimed to investigate whether color differences in the stained cavity floor simulating recurrent caries can be detected using various restored resin composites. Artificial teeth were made with conventional resin composite (Estellite Sigma Quick A3; ET), and class V cavities were prepared. To simulate the color of caries, a dot was marked in the center of the cavity floor using four different magic pens. The cavities were filled with ET and two universal shade resin composites (UC, Omnichroma; OMI, Essentia Universal; ESS). For photographic analysis, a standard correction color chart was used. The color difference (ΔE00) between (i) the lab value at the center of the cavity and (ii) the average lab value at 1 mm mesial and distal to the center of the cavity was calculated. The data were statistically746o-way ANOVA (p < 0.05). For visual analysis, 25 dentists were asked to complete a questionnaire to determine whether the color differences were noticeable. In the photographic analysis, UC showed larger ΔE00 values than ET in all colors. Visual analysis revealed higher detection rates for UC than ET. Universal shade resin composite tends to reflect the color of the cavity more effectively than conventional resin composite.
• Evaluation of caries risk assessment practices among dental practitioners in Guangzhou, China: a cross-sectional study.
  Ermin Nie; Rui Jiang; Rafiqul Islam; Xiang Li; Jiali Yu
  Frontiers in oral health, 5, 1458188, 1458188, 2024年10月, ［国際誌］
  英語, 研究論文（学術雑誌）, INTRODUCTION: This cross-sectional study aimed to investigate dental practitioners' knowledge and practices regarding Caries risk assessment (CRA) in routine clinical practice in Guangzhou, China. METHODS: An online questionnaire was disseminated to dental practitioners to gather socio-demographic information, factors associated with CRA, the implementation of preventive treatment, and the level of awareness regarding personalized preventive treatment in relation to CRA. Statistical analyses included descriptive statistics, Chi-square tests, ANOVA, MANCOVA, linear regression, and scatter plots. RESULTS AND DISCUSSION: Out of the 695 dental practitioners who were contacted, 206 dentists participated in the online survey. However, out of the total number of dentists, 198 were successfully recruited, while the remaining 8 dentists had incomplete data in their questionnaires. 92.4% of dentists provided in-office fluoride treatments, and 73.2% held a strong belief in the correlation between current oral hygiene and tooth cavities. 23.7% of dentists evaluated caries risk on an individual basis, and a significant 41.9% never utilized a particular type of CRA. 53.5% of dentists recommended non-prescription fluoride rinses, whereas 51% advocated prescription fluoride treatments. Significant statistical relationships were found between the use of in-office fluoride and the effectiveness of restorative treatment (P < 0.05). Additionally, a significant association was discovered between the use of a specific form for CRA and the kind of dental school (P < 0.05). The study suggests that a significant number of dental practitioners in Guangzhou, China, do not utilize dedicated assessment forms for CRA in their routine professional activities. These findings highlight the im-portance of encouraging dentists to utilize CRA systems to effectively identify patients who are at risk of acquiring dental caries.
• Biological evaluation of novel phosphorylated pullulan‐based calcium hydroxide formulations as direct pulp capping materials: An in vivo study on a rat model
  Md Refat Readul Islam; Rafiqul Islam; Yunqing Liu; Yu Toida; Yasuhiro Yoshida; Hidehiko Sano; Hany Mohamed Aly Ahmed; Atsushi Tomokiyo
  International Endodontic Journal, Wiley, 2024年05月23日
  研究論文（学術雑誌）, Abstract
  
  Aim
  
  Calcium hydroxide (CH) has been considered as a direct pulp capping materials (DPC) for the last decades despite having some limitations. Phosphorylate pullulan (PPL) incorporated with CH (CHPPL) is a novel biomaterial that was introduced as a promising DPC material. Thus, the aim of the study was to evaluate the inflammatory response and mineralized tissue formation (MTF) ability of PPL‐based CH formulations on rat molars after DPC.
  
  Methodology
  
  This study consisted of six groups: CH with 1% PPL (CHPPL‐1); 3% PPL (CHPPL‐3); 5% PPL (CHPPL‐5); Dycal and NEX MTA Cement (N‐MTA) as the positive control, and no capping materials (NC). One hundred twenty maxillary first molar cavities were prepared on Wistar rats. After capping, all the cavities were restored with 4‐META/MMA‐TBB resin and pulpal responses were evaluated at days 1, 7, and 28. Kruskal–Wallis followed by Mann–Whitney U‐test was performed with a significance level of 0.05. Immunohistochemical expression of IL‐6, Nestin, and DMP‐1 was observed.
  
  Results
  
  At day 1, CHPPL‐1, N‐MTA, and Dycal exhibited no to mild inflammation, whilst CHPPL‐3, CHPPL‐5, and NC showed mild to moderate inflammation, and the results were significantly different (p < .05). At day 7, mild to moderate inflammation was observed in CHPPL‐1, N‐MTA, and Dycal, whereas CHPPL‐3, CHPPL‐5, and NC exhibited moderate to severe inflammation. Significant differences were observed between CHPPL‐1 and N‐MTA with NC (p < .05), CHPPL‐1 and CHPPL‐3 with CHPPL‐5 and Dycal (p < .05), and CHPPL‐3 with N‐MTA (p < .05). A thin layer of mineralized tissue formation (MTF) was observed in all groups. At day 28, CHPPL‐1, Dycal, and N‐MTA showed no to mild inflammation, whilst CHPPL‐3, CHPPL‐5, and NC exhibited mild to severe inflammation, and statistically significant difference was detected (p < .05). CHPPL‐1, Dycal, and N‐MTA exhibited continuous MTF, whilst CHPPL‐3, CHPPL‐5, and NC had thicker and interrupted MTF. Significant differences were observed between CHPPL‐1, CHPPL‐3, and N‐MTA with NC group (p < .05). Variable expressions of IL‐6, Nestin, and DMP‐1 indicated differences in the materials' impact on odontoblast‐like cell formation and tissue mineralization.
  
  Conclusions
  
  These findings suggest that CHPPL‐1 has the potential to minimize pulpal inflammation and promote MTF and had similar efficacy as MTA cement.
• Direct pulp capping procedures – Evidence and practice
  Rafiqul Islam; Md Refat Readul Islam; Toru Tanaka; Mohammad Khursheed Alam; Hany Mohamed Aly Ahmed; Hidehiko Sano
  Japanese Dental Science Review, 59, 48, 61, Elsevier BV, 2023年12月
  研究論文（学術雑誌）
• Effects of Short-Term Exposure of Chloramine-T Solution on the Characteristics of Light-Cured and Chemical-Cured Adhesives
  Yunqing Liu; Norihito Sakaguchi; Masahiro Iijima; Md Refat Readul Islam; Jiayuan Zhang; Rafiqul Islam; Monica Yamauti; Hidehiko Sano; Atsushi Tomokiyo
  Polymers, 15, 19, 3995, 3995, MDPI AG, 2023年10月05日
  研究論文（学術雑誌）, This study evaluated the effect of a 0.5% chloramine T solution on a chemical-cured universal adhesive by comparing the light-cured, one-step, self-etch adhesive for the bonding performance, mechanical properties, and resin–dentin interfacial characteristics. Caries-free human molars were randomly assigned into eight groups based on the bonding systems employed (Bond Force II, BF and Bondmer Lightless, BL), the immersion solutions used before bonding (0.5% chloramine T solution and distilled water), and the immersion durations (5 and 60 min). Microtensile bond strength (μTBS), nanoleakage evaluation, and nanoindentation tests were performed, and the surface morphology of the resin–dentin interface was examined using a focus ion beam/scanning ion microscopy system. Immersion in chloramine-T for 5 min significantly decreased the μTBS of Bondmer Lightless (from 22.62 to 12.87 MPa) compared with that in distilled water. Moreover, there was also a decreasing trend after immersing in chloramine-T for 60 min (from 19.11 to 13.93 MPa). Chloramine T was found to have no effect on the hardness, elastic modulus, or morphological characteristics of the ion-beam milled resin–dentin interfacial surfaces in the tested adhesives, suggesting that chloramine T might reduce the bond strength by interfering with the interaction and the sealing between the adhesive resin and dentin in the chemical-cured universal adhesive, albeit without affecting the mechanical properties.
• Dental Caries Prevention Knowledge, Attitudes, and Practice among Patients at a University Hospital in Guangzhou, China.
  Rui Jiang; Jiali Yu; Rafiqul Islam; Xiang Li; Ermin Nie
  Medicina (Kaunas, Lithuania), 59, 9, 2023年08月28日, ［国際誌］
  英語, 研究論文（学術雑誌）, Background and Objectives: This study aimed to assess the knowledge, attitudes, and practice (KAP) of patients regarding dental caries prevention in a university hospital in Guangzhou, China. Materials and Methods: A hospital-based KAP survey was conducted in a cross-sectional manner at the First Affiliated Hospital in Guangzhou, China, from 1 September to 30 September 2022. Questionnaires were distributed to eligible patients, resulting in the participation of 251 individuals. The questionnaire consisted of five sections capturing socio-demographic data and exploring participants' knowledge, attitudes, practice, and accurate preventive dental caries knowledge. Descriptive statistics and a generalized linear model with univariate tests were used for analysis. Results: The study results show that the knowledge score 7.97 (±0.91) and attitudes score 7.67 (±0.89) among the participants were good while the practice score was 6.80 (±0.81) moderate. The majority of participants identified tooth infections (81.3%), bacteria (92%), and sugar (89.2%) as the main causes of gum bleeding and tooth decay. Brushing (96%) and fluoride (80.5%) were also recognized as essential for preventing tooth decay. Of oral diseases, 94% were recognized as potentially affecting overall health. The vast majority ranging from 92.8% to 98%, believed that oral health is crucial to overall health and that regular check-ups and proper brushing habits are beneficial. There is a significant association of gender with age (p = 0.018), occupation (p = 0.050), lifestyle habit (p = 0.012), and knowledge score; whole education is significantly associated with practice score (p = 0.050). Conclusions: The majority of patients exhibited accurate knowledge and attitudes with moderate practice towards dental caries prevention, with socio-demographic factors playing a major role. However, effective implementation of dental health education programs across the healthcare system is still required to further enhance outcomes.
• The effect of an extra hydrophobic resin layer on the bond strength and durability of one-step universal adhesives used as a primer
  Linhong Wang; Xiaolei Bai; Yunqing Liu; Rafiqul Islam; Shuhei Hoshika; Hidehiko Sano; Fan Yang
  Journal of Dentistry, 135, 104568, 104568, Elsevier BV, 2023年08月
  研究論文（学術雑誌）
• Effect of sodium hypochlorite on bonding performance of universal adhesives to pulp chamber dentin
  Rafiqul Islam
  Journal of Dental Sciences, 2023年
  研究論文（学術雑誌）
• Influence of Novel Experimental Light-Cured Resin Cement on Microtensile Bond Strength
  Midori Kawamura; Yu Toida; Shuhei Hoshika; Md Refat Readul Islam; Yitong Li; Ye Yao; Yunqing Liu; Rafiqul Islam; Takaaki Sato; Yasushi Shimada; Hidehiko Sano
  Polymers, 14, 19, 4075, 4075, MDPI AG, 2022年09月28日, ［国際誌］
  英語, 研究論文（学術雑誌）, The purpose of this study was to evaluate the microtensile bond strength (µTBS) and Knoop hardness number (KHN) of a novel experimental light-cured resin cement (HL). Eighteen flat dentin surfaces of human molars were polished using #600 SiC paper and bonded to CAD/CAM resin blocks with the respective resin cements and composites: HL, Panavia V5 (PV), and Clearfil AP-X (AP). All specimens were stored in distilled water at 37 °C for 24 h and 7 days. Scanning electron microscope (SEM) and energy dispersive X-ray (EDX) observations were performed to evaluate filler morphology and to detect the elements. The resin cements had a significant effect on the immediate µTBS (F = 22.59, p < 0.05) and after water storage µTBS (F = 22.83, p < 0.05). Significant differences (p < 0.05) in the KHN between the tested materials were observed, and HL indicated the highest KHN when compared with PV. HL showed a combination of the regular-shaped filler and spherical-shaped filler within the matrix. Silicon was detected in HL from the EDX evaluation. HL exhibited better bonding performance and polymerization, which may have contributed to the improvement of the adhesive strength.
• Teledentistry as an Effective Tool for the Communication Improvement between Dentists and Patients: An Overview
  Md Refat Readul Islam; Rafiqul Islam; Sultana Ferdous; Chiharu Watanabe; Monica Yamauti; Mohammad Khursheed Alam; Hidehiko Sano
  Healthcare, 10, 8, 1586, 1586, MDPI AG, 2022年08月21日, ［国際誌］
  英語, 研究論文（学術雑誌）, Teledentistry is an online dental care service that allows patients and dentists to meet in real time, safely, without being at the same location. During the COVID-19 pandemic, real-time videoconferencing has gained popularity in the field of teledentistry, with numerous benefits for both patients and dentists. Online consultations can minimize costs, maximize time, and provide more convenient care options for both patients and dentists. When practicing teledentistry, a dentist must establish a good doctor–patient relationship. Dentists must ensure that the telecommunication solution that they choose meets their clinical requirements and complies with privacy laws. Dentists should provide adequate information to patients about the limitations, advantages, and disadvantages that may occur during online consultation. Dentists must follow guidelines and procedures regarding informed consent, patient details, personal communications, and consultancies’ privacy and confidentiality. The patient should be aware of the limitations of teledentistry, and dentists will provide the best advice possible in the absence of a face-to-face consultation. This article discusses how teledentistry could be an effective tool for dentists and patients.
• Evaluation of Color and Spectral Behavior of a Novel Flowable Resin Composite after Water Aging: An In Vitro Study.
  Fei Chen; Di Wu; Rafiqul Islam; Yu Toida; Chiharu Kawamoto; Monica Yamauti; Hidehiko Sano
  Materials (Basel, Switzerland), 15, 12, 2022年06月09日, ［国際誌］
  英語, 研究論文（学術雑誌）, BACKGROUND: This study aimed to evaluate the color matching, light transmittance, and reflectance characteristics of the novel flowable resin composite OCF-001 (OCF). METHODS: Fifty-four resin composite molds were made with simulated class I cavities of A2, A3, and A4 shades by filling the rubber mold interspace with Estelite Sigma Quick (ESQ), Gracefil Putty (GP) and Filtek Supremme Ultra (FSU). After applying the adhesive, three different flowable resin composites (n = 6), OCF, Gracefil LoFlo (GLF), and Supreme Ultra Flowable (SUF), were used to fill the cavities. A colorimeter was used to measure the color parameters (CIEDE2000). The color measurements were taken immediately and after 28 days. Data were analyzed using the nonparametric Kruskal-Wallis (α = 0.05) and Wilcoxon tests. The light transmittance and reflection characteristics were measured with a black background using a spectrophotometer under D65 illumination. RESULTS: The ΔE00, and ΔC of OCF was lower than other tested materials in A2 and A3 shades both immediately and after 28 days. OCF showed the highest transmittance characteristic, and a relatively stable reflectance curve in all the wavelengths. CONCLUSIONS: OCF showed better shade matching with the surrounding shades of A2 and A3, a relative uniform reflectance and higher light transmission properties.
• A novel method of 3D printing locating guide for abutment screw removal in cement-retained implant-supported prostheses
  Jiali Yu; Yanbin Chen; Xiangzhen Liu; Rafiqul Islam; Mohammad Khursheed Alam
  Journal of Dental Sciences, 17, 4, 1665, 1668, Elsevier {BV}, 2022年03月
  英語, 研究論文（学術雑誌）
• Pulpal response to mineral trioxide aggregate containing phosphorylated pullulan-based capping material
  Yu TOIDA; Shimpei KAWANO; Rafiqul ISLAM; Fu JIALE; AFM A CHOWDHURY; Shuhei HOSHIKA; Yasushi SHIMADA; Junji TAGAMI; Masahiro YOSHIYAMA; Satoshi INOUE; Ricardo M. CARVALHO; Yasuhiro YOSHIDA; Hidehiko SANO
  Dental Materials Journal, 41, 1, 126, 133, Japanese Society for Dental Materials and Devices, 2022年01月25日, ［国内誌］
  英語, 研究論文（学術雑誌）, This study aimed to evaluate the pulpal responses of monkey's pulp after direct pulp capping (DPC) with the novel mineral trioxide aggregate containing phosphorylated pullulan-based material (MTAPPL). Seventy-two teeth were randomly divided into four groups: MTAPPL; Nex-Cem MTA (NX); TheraCal LC (TH); and Dycal (DY). Histopathological changes in the pulps were observed at days 3, 7 and 70. On day 3, mild inflammatory responses were observed in the MTAPPL, no to moderate inflammatory responses in the TH, whereas moderate inflammatory responses in the NX and DY. No mineralized tissue formation (MTF) was observed in all groups. On day 7, no or mild inflammatory responses were observed in all groups. Initial MTF was observed except for DY. No inflammation with complete MTF including presence of odontoblast-like cells was observed in the MTAPPL, NX and TH groups at day 70. These findings indicate that MTAPPL could be an efficient DPC material.
• Effectiveness of Direct Pulp Capping Bioactive Materials in Dentin Regeneration: A Systematic Review
  Ermin Nie; Jiali Yu; Rui Jiang; Xiangzhen Liu; Xiang Li; Rafiqul Islam; Mohammad Khursheed Alam
  Materials, 14, 22, 6811, 6811, {MDPI} {AG}, 2021年11月11日
  英語, 研究論文（学術雑誌）, Background: Regenerative endodontics aims to restore normal pulp function in necrotic and infected teeth, restoring protective functions, such as innate pulp immunity, pulp repair through mineralization, and pulp sensibility. The aim of this systematic review was to assess the dentin regeneration efficacy of direct pulp capping (DPC) biomaterials. Methods: The literature published between 2005 and 2021 was searched by using PubMed, Web of Science, Science Direct, Google Scholar, and Scopus databases. Clinical controlled trials, randomized controlled trials, and animal studies investigating DPC outcomes or comparing different capping materials after pulp exposure were included in this systematic review. Three independent authors performed the searches, and information was extracted by using a structured data format. Results: A total of forty studies (21 from humans and 19 from animals) were included in this systemic review. Histological examinations showed complete/partial/incomplete dentin bridge/reparative dentin formation during the pulp healing process at different follow-up periods, using different capping materials. Conclusions: Mineral trioxide aggregate (MTA) and Biodentine can induce dentin regeneration when applied over exposed pulp. This systematic review can conclude that MTA and its variants have better efficacy in the DPC procedure for dentin regeneration.
• Histological evaluation of a novel phosphorylated pullulan‐based pulp capping material: An in vivo study on rat molars
  Rafiqul Islam; Yu Toida; Fei Chen; Toru Tanaka; Satoshi Inoue; Tetsuya Kitamura; Yasuhiro Yoshida; Abu Faem Mohammad Almas Chowdhury; Hany Mohamed Aly Ahmed; Hidehiko Sano
  International Endodontic Journal, 54, 10, 1902, 1914, Wiley, 2021年10月, ［国際誌］
  英語, 研究論文（学術雑誌）, AIM: To evaluate the dental pulp response to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct pulp capping. METHODS: Ninety-six cavities were prepared in the maxillary first molars of 56 male Wistar rats. The dental pulps were intentionally exposed and randomly divided into four groups according to the application of pulp capping materials: MTAPPL; phosphorylated pullulan (PPL); a conventional MTA (Nex-Cem MTA, NCMTA; positive control); and Super-Bond (SB; negative control). All cavities were restored with SB and observed for pulpal responses at 1-, 3-, 7- and 28-day intervals using a histological scoring system. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U-test with Bonferroni's correction, and the level of significance was set at 0.05. DMP1 and CD34 antigen were used to evaluate odontoblast differentiation and pulpal vascularization, respectively. RESULTS: On day 1, mild inflammatory cells were present in MTAPPL and NCMTA groups; fewer inflammatory cells were present in the PPL, whereas SB was associated with a mild-to-moderate inflammatory response. A significant difference was observed between PPL and SB (p < .05). No mineralized tissue deposition was observed. On day 3, moderate-to-severe inflammatory cells were present in PPL and SB, whereas MTAPPL and NCMTA had a mild inflammatory response. Initial mineralized tissue deposition was observed in the NCMTA, MTAPPL and SB. A significant difference was observed between MTAPPL and PPL (p < .05). On day 7, a thin layer of mineralized tissue was observed in all tested groups with no or mild inflammatory response. On day 28, no inflammatory response was observed in MTAPPL, whereas NCMTA, PPL and SB had mild inflammatory responses. A significant difference was observed between MTAPPL and SB (p < .05). Complete mineralized tissue barrier formation was observed in MTAPPL, NCMTA and PPL with no significant difference (p > .05). SB exhibited incomplete mineralized tissue barriers, significantly different from NCMTA, MTAPPL and PPL (p < .05). The staining with CD34 was positive in all the groups on all observation days. CONCLUSION: The favourable pulpal responses and induction of mineralized tissue formation associated with MTAPPL indicate its potential application as a direct pulp capping material.
• Evaluation of shade matching of a novel supra‐nano filled esthetic resin composite employing structural color using simplified simulated clinical cavities
  Fei Chen; Yu Toida; Rafiqul Islam; Arefin Alam; Abu Faem Mohammad Almas Chowdhury; Monica Yamauti; Hidehiko Sano
  Journal of Esthetic and Restorative Dentistry, 33, 6, 874, 883, Wiley, 2021年09月, ［国際誌］
  英語, 研究論文（学術雑誌）, OBJECTIVE: To evaluate the shade matching ability of a novel supra-nano filled esthetic resin composite employing structural color technology using simplified simulated clinical cavities. Filler morphology and light transmittance characteristics were also evaluated. MATERIALS AND METHODS: One-hundred and twenty frames of resin composite were built in A1, A2, A3, and A4 shades to simulate Class I cavities (diameter = 4 mm, height = 2 mm). For each shaded frame, cavities were filled with three different types of filler containing resin composites (n = 10): supra-nano filled (SN filled) resin composite, microhybrid filled (MH filled) resin composite, and clustered-nano filled (CN filled) resin composite. Color parameters were calculated using CIELAB (△Eab ) and CIEDE2000 (△E00 ). Data were analyzed using one-way analysis of variance (ANOVA), followed by Duncan's test (α = .05). Filler morphology and light transmittance characteristics were measured to explore the role of structural color on shade matching. RESULTS: △Eab and △E00 of SN filled resin composite were significantly lower in A2, A3, and A4 shades (P < .05). CONCLUSIONS: The SN filled resin composite showed better shade matching with A2, A3, and A4 shades of resin composite frames compared to MH filled resin composite, and CN filled resin composite. CLINICAL SIGNIFICANCE: Universal-shade resin composites, which were expected to match nearly all shades, simplify the restorative procedure. Resin composite, which contained spherical supra-nano filler particles, could contribute most to its shade matching by stimulating structural color. Structural color technology may provide additional benefits for shade matching of resin composites.
• 歯科用汎用接着剤の肥厚度と機械的特性は象牙質に対する接着強度に影響を及ぼしうる(Thickness and mechanical properties of universal adhesives can affect their bond strength to dentin)
  Arefin Alam; Rafiqul Islam; Akter Rime Shamme; Readul Islam Md Refat; Matsumoto Mariko; Hoshika Shuhei; Kawamoto Chiharu; Ikeda Takatsumi; Tanaka Toru; Yamauti Monica; Chowdhury A.F.M. Almas; Sano Hidehiko
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 28, 28, (NPO)日本歯科保存学会, 2020年06月
  英語
• Variable Smear Layer and Adhesive Application: The Pursuit of Clinical Relevance in Bond Strength Testing
  Abu Faem Mohammad Almas Chowdhury; Rafiqul Islam; Arefin Alam; Mariko Matsumoto; Monica Yamauti; Ricardo Marins Carvalho; Hidehiko Sano
  International Journal of Molecular Sciences, 20, 21, 5381, 5381, MDPI AG, 2019年10月29日, ［国際誌］
  英語, 研究論文（学術雑誌）, The removal or modification of smear layers that cover the dentin is critical to allow the penetration of adhesive molecules and to ensure a strong bond between resin and dentin. Aiming to establish a model for clinically-relevant dentin-bond testing, we evaluated the effects of smear layers created by abrasives having similar coarseness (180-grit SiC paper; fine-grit diamond bur) and application modes (single application; double application) on the microtensile bond strengths (µTBS) of two currently available universal adhesives (G-Premio Bond; Scotchbond Universal Adhesive) and a two-step self-etch adhesive (Clearfil Megabond 2). Sixty extracted human third molars were used for the μTBS test. Data were analyzed by three-way ANOVA and Tukey’s test (α = 0.05). Fracture modes were determined using stereomicroscopy. An additional 24 third molars were prepared for observation of the resin–dentin interface by TEM and adhesive-smear layer interaction by SEM. μTBS was significantly affected by the adhesives and their application modes (p < 0.001), implying that the double application of universal adhesives should be recommended to improve their performance. The effect of smear layers was not significant (p > 0.05), indicating that 180-grit SiC papers could be used to prepare dentin as a substitute for fine-grit diamond burs for dentin-bond testing in laboratory settings.
• Global Dental Arch Dimension Norms and Sexual Disparities: An overview
  Rafiqul Islam; Mohammad Khursheed Alam; Fazal Shahid; Mohd; Fadhli Khamis
  Bangladesh Journal of Medical Science, 18, 1, 30, 35, Bangladesh Journals Online ({JOL}), 2018年12月30日
  研究論文（学術雑誌）, Background: The aim of the present study was to do an overview of the arch dimension for various populations. Also, to evaluate the various type of measurement methods and sexual disparities. Two reviewers independently performed the selection process and the quality of studies was assessed.
  Method: Studies published form January 2000 until October 2015 were identified in electronic databases: Pubmed, Scopus, Science direct, Web of Science, Medpilot and Medline using keywords.Criteria used included: observational studies, Arch size measured, Permanent dentition, Arch dimension investigated via plaster and digital dental models, measurement via calipers and computers software’s.
  Result: The forest plots showed the continuous trend for the greater arch dimension for male in relation to female. The current overview showed the arch dimension and its disparities for various populations.
  Conclusion: The various methods to assess arch dimension should be carefully considered and well conducted as part of the clinical assessment of orthodontic treatment, since arch dimension could influence the diagnosis and treatment planning of orthodontist.
  Bangladesh Journal of Medical Science Vol.18(1) 2019 p.30-35
• Treatment of Angle class I malocclusion with severe crowding by extraction of four premolars: A case report
  Mohammad Khursheed Alam; Shifat A Nowrin; Fazal Shahid; Sanjida Haque; Anas Imran; Nashid Fareen; Mamun Khan Sujon; Shaila Zaman; Rafiqul Islam; Shamima Easmin Nishi
  Bangladesh Journal of Medical Science, 17, 4, 683, 687, Bangladesh Journals Online ({JOL}), 2018年09月19日
  研究論文（学術雑誌）, Aims: To correct the Angle class I malocclusion with crowding via four premolar extractions.Presentation of case:A 19-year-old Malaysian female presented with the bimaxillary protrusion, crowding, high canines, midline deviation and edge-to-edge anterior bite. Upper lateral incisors were palatalized and in crossbite interactions with the opposite teeth.Discussion: Treatment was initiated using fixed orthodontic appliances followed by four 1st premolar extractions in both arches. Case was finished with good inter cuspation of the upper and lower teeth. Molars were finished in a Class I relationship with canine guidance, and ideal overjet and overbite were obtained.Conclusion: Successful finishing of a case with beautiful smile, obtained proper overjet and overbite, corrected cross bite, midline shifting and the improved soft tissue profile.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.683-687
• Class III malocclusion with anterior open bite, orthodontic treatment in an adult patient: a clinical note.
  Rafiqul Islam
  International Medical Journal, 2017年08月
  研究論文（学術雑誌）
• Spaced Dentition Corrected by Fixed Orthodontic Treatment: A Case Series
  Rafiqul Islam
  International Medical Journal, 2017年08月
  研究論文（学術雑誌）
• Multi-disciplinary approach of bleaching: clinical note.
  Rafiqul Islam
  International Medical Journal, 2017年02月
  研究論文（学術雑誌）
• Headgear Appliance-A Contemporary Treatment Method: A Review.
  Rafiqul Islam
  International Medical Journal, 2017年02月
  研究論文（学術雑誌）
• Morphometric Analysis of Tooth Size and its Relationship with BMI in Transgender Population : A New Exposure in Dentistry
  Islam Rafiqul; Alam Mohammad Khursheed; Mukai Ayako; Murakami Yumiko; Shoumura Masahito; Osuga Naoto; Khamis Mohd Fadhli
  Journal of hard tissue biology, 26, 4, 361, 368, The Hard Tissue Biology Network Association, 2017年
  英語, 研究論文（学術雑誌）
• White Spot Lesion, the Silent Factor Killing Your Smile: An Overview
  Rafiqul Islam; Mohammad Alam
  British Journal of Medicine and Medical Research, 17, 6, 1, 7, Sciencedomain International, 2016年01月10日
  研究論文（学術雑誌）
• Medical and dental problems among transgenders - An untouched topic: A review
  Rafiqul Islam
  International Journal of Pharma & Bio Science,, 2015年
  研究論文（学術雑誌）

• FGF2と新規ケイ酸カルシウム系材料を用いた生活歯髄切断法の基礎的研究
  近 加名代; 北村 哲也; 松田 彩; 間石 奈湖; Islam Rafiqul; 戸井田 侑; 佐野 英彦; 樋田 京子, 北海道歯学雑誌, 41, 2, 132, 142, 2021年03月
  北海道歯学会, 日本語

• Dental Pulp Revascularization and Regeneration: Advances in Bioactive Nanomaterials and Bioceramics.
  Islam R.; Tomokiyo A.
  The 4th International Conference on Japan−Bangladesh Research and Practice 2025, 2025年12月14日
  ［招待講演］
• Effects of vital pulp therapy on dentinogenesis and pulpitis with defective restoration.
  Islam R.; Tsichiya K.; Toida Yu; Kaneko H.; Hoshika S.; Kawamoto C.; Sano H.; Tomokiyo A.
  163rd Hozon Gakkai, Japan (2025), 2025年11月07日, ポスター発表
• Preserving the Pulp: Materials in Direct Pulp Capping.
  Islam R.
  Hokkaido University Summer Institute 2025, 2025年07月17日
  ［招待講演］
• Evaluating the Efficacy of Colloidal Platinum Nanoparticle and Mineral Trioxide Aggregate Combinations in Dentin Regeneration.
  The 72nd Annual Meeting of Japanese Association for Dental Research, 2024年11月
• In vivo assessment of bioceramics in pulp capping : A comparatative study
  The 160th meeting of the Japanese society of conservative dentistry, Sendai, 2024年06月16日, ポスター発表
• Colloidal platinum nanoparticles for dental pulp revascularization: an immunohistochemical evaluation.
  62nd Annual Scientific Meeting of the IADR ANZ Division, Sydney, New South Wales, Australia, 2023年09月27日
• Colloidal Platinum Nanoparticles Enhance the Healing Potential of Dental Pulp.
  Islam R; Toida Y; Islam MRR; Tanaka T; Chowdhury AFMA; Ahmed HMA; Sano H
  2022 IADR/APR General Session & Exhibition, China, 2022年07月25日, 英語, 口頭発表（一般）
• Application Mode Affects the Bond Strength of Self-etch Adhesives.
  Chowdhury AFMA; Alam A; Rime SA; Islam R; Monica Y; Hidehiko S
  IADR/AADR/CADR General Session 98th General Session, Washington D.C., USA (2020)., 2020年, 英語, 口頭発表（一般）
• Effects of Colloidal Platinum Nanoparticles on Dental Pulp.
  Islam R; Chowdhury AFMA; Toida Y; Chen F; Shuhei H; Takatsumi I; Chiharu W; Toru T; Mariko M; Yamauti M; Sano H
  IADR/AADR/CADR General Session 98th General Session, Washington D.C. USA (2020)., 2020年, 英語, 口頭発表（一般）
• Light Diffusibility evaluation and filler particle morphology observation of newly developed composite resin (OMNICHROMA).
  Chen F; Yu T; Islam R; Mariko M; Kawamoto C; Sano H
  150th Hozon Gakkai, Japan (2019)., 2019年, 日本語, 口頭発表（一般）
• Structural Color: New Concept of Color Matching of Resin Composite.
  Chen F; Yu T; Islam R; Alam A; Chowdhury AFM; Yamauti M; Sano H
  2019 IADR/AADR/CADR General Session & Exhibition, Canada (2019)., 2019年, 英語, 口頭発表（一般）
• Color Matching of Newly Developed Composite Resin (ECM-001).
  Chen F; Yu T; Islam R; Kawamoto C; Ikeda T; Sano H
  149th Hozon Gakkai, Japan (2018)., 2018年, 日本語, 口頭発表（一般）
• Effect of resin cements and ceramic types on the translucency and color stability of laminate veneers.
  Yamauti M; Silame FDJ; Tonani R; Antunes RAP; Souza FCPP; Islam R; Alam A; Sano H
  2018 KACD Scientific Congress and 20th KACD-JSCD Joint Scientific Meeting, Korea (2018)., 2018年, 英語, ポスター発表
• Evaluation of the effect of ɣ-radiation on the mechanical properties of dentin.
  Chowdhury AFMA; Alam A; Saikaew P; Eamsa-ard P; Islam R; Toida Y; Chen F; Kawano S; Matsumoto M; Hoshika S; Yamauti M; Sano H
  The 66th Annual Meeting of Japanese Association for Dental Research., Japan (2018)., 2018年, 英語, ポスター発表
• Evaluation of a novel direct pulp capping material containing phosphorylated pullulan.
  Islam R; Yu T; Chen F; Chowdhury AFMA; Tetsuya K; Toru T; Tadashi I; Hida K; Yasuhiro Y; Sano H
  The 66th Annual Meeting of Japanese Association for Dental Research, Hokkaido, Japan (2018)., 2018年, 英語, ポスター発表
• The effect of remaining dentin thickness on dentin bonding for self-ethcing adhesives.
  Rime SA; Matsumoto M; Ahmed Z; Ting S; Pipop S; Pimpinee E; Almas C; Islam R; Toida Y; Kakuda S; Hoshika S; Kawamoto C; Ikeda T; Tanaka T; Sano H
  148th Hozon Gakkai, Japan (2017)., 2017年, 英語, 口頭発表（一般）
• Morphometric analysis of tooth size and its relationship with Body Mass Index (BMI) in transgender population: a new exposure in dentistry.
  Islam R; Alam MK; Khamis MF
  21st National Conference on Medical and Health Science, Malaysia (2016)., 2016年, 英語, 口頭発表（一般）
• Medical and Dental Problems in Transgenders: A Taboo Unveiled.
  Islam R; Alam MK; Khamis MF
  International Symposium on Allied Health Science, Malaysia (2015)., 2015年, 英語, 口頭発表（一般）

• 接着歯学研究Ⅰ, 2024年, 博士後期課程, 歯学院
• 接着歯学研究Ⅱ, 2024年, 博士後期課程, 歯学院
• 歯内療法学, 2024年, 博士後期課程, 歯学院
• 歯内療法学研究Ⅰ, 2024年, 博士後期課程, 歯学院
• 歯内療法学研究Ⅱ, 2024年, 博士後期課程, 歯学院
• 歯冠修復学研究Ⅰ, 2024年, 博士後期課程, 歯学院
• 歯冠修復学研究Ⅱ, 2024年, 博士後期課程, 歯学院
• 齲蝕制御・保存修復学, 2024年, 博士後期課程, 歯学院
• 保存修復学基礎実習, 2024年, 学士課程, 歯学部
• 保存修復学Ⅰ, 2024年, 学士課程, 歯学部
• 保存修復学Ⅱ, 2024年, 学士課程, 歯学部

• 現在
  日本歯科保存学会

• Assistant Professor
  Hokkaido University, 2022年10月01日 - 現在, ［教材］
• Postdoctoral Research Fellow
  Hokkaido University, 2021年09月01日 - 2022年09月30日, ［教材］
• Academic Fellow
  Hokkaido University, 2021年04月01日 - 2021年08月31日, ［その他］

• メタゲノム解析・光工学・AIを融合した革新的細菌可視化法の創出とう蝕治療への応用
  科学研究費助成事業
  2026年04月01日 - 2030年03月31日
  福山 麻衣; ISLAM RAFIQUL; 友清 淳; 戸井田 侑; 星加 修平; 川本 千春; 槌谷 賢太; 兼子 裕史; 長谷部 晃
  日本学術振興会, 基盤研究(C), 北海道大学, 26K12674
• マテリアルズ・インフォマティクスの応用による多機能型接着性レジンセメントの創生
  科学研究費助成事業
  2026年04月01日 - 2029年03月31日
  星加 修平; 坂口 紀史; 友清 淳; 川本 千春; 戸井田 侑; 兼子 裕史; 槌谷 賢太; ISLAM RAFIQUL; 福山 麻衣
  日本学術振興会, 基盤研究(C), 北海道大学, 26K12673
• 低分子量ヒアルロン酸および接着性MTAを基盤とする次世代型Revascularizationの確立
  科学研究費助成事業
  2025年04月01日 - 2029年03月31日
  ISLAM RAFIQUL; 友清 淳; 戸井田 侑; 星加 修平; 川本 千春; 佐野 英彦
  日本学術振興会, 基盤研究(C), 北海道大学, 25K13029
• iPS創薬の新領域を拓く神経堤細胞賦活化化合物の創出と歯周組織再生への応用
  科学研究費助成事業
  2025年06月27日 - 2028年03月31日
  友清 淳; ISLAM RAFIQUL; 戸井田 侑; 星加 修平; 川本 千春
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 25K22677
• The combined application of mineral trioxide aggregate containing phosphorylated pullulan and colloidal platinum nanoparticles to control inflammation and facilitate dental pulp regeneration
  科学研究費助成事業 研究活動スタート支援
  2021年08月30日 - 2023年03月31日
  ISLAM RAFIQUL
  We evaluated the healing response of three different Colloidal platinum nanoparticles (CPN) materials in rodent dental pulp at day 1, 3 and 7 after direct capping. The teeth pulps were intentionally exposed and randomly divided into three groups according to the application of the CPN materials: group 1- PAA-PT (platinum, poly acrylic acid, water), group 2- C-PT (platinum, sodium citric acid, water) and group 3- C-CYD (platinum, sodium citric acid, γ-cyclodextrin, water).Additional two groups were included as positive control (Mineral Trioxide Aggregate-MTA) and negative control (Super Bond-SB). At day 1, no or mild inflammatory responses were observed in all three groups of CPN, which were significantly different to the MTA and SB groups (P<0.05), except C-PT group. At day 3, numerous blood vessels with no or mild inflammation were observed in the C- CYD group, indicating rapid angiogenesis, which was significantly different from MTA and SB groups (P<0.05), whereas PAA-PT and C-PT groups showed mild to moderate inflammatory responses. At day 7, no or mild inflammation was observed in the C-CYD group which was significantly different from PAA-PT, C-PT and SB (P<0.05), whereas mild or moderate inflammatory responses were observed in all other groups.
  
  
  Our observations have been accepted for the conference presentation in the 100th General Session of the IADR/APR, 2022 (Abstract ID: 3723202; Title: Colloidal platinum nanoparticles enhance the healing potential of dental pulp).
  日本学術振興会, 研究活動スタート支援, 北海道大学, 21K21028

• Editorial Board Member - Scientific Reports
  審査・評価, 査読
  学会・研究会等
• Editorial Board Member - BMC Oral Health
  審査・評価, 査読
  学会・研究会等