Researcher basic information

• Ph. D (Enginieering), Hokkaido University, Hokkaido University

• https://researchmap.jp/ktajima_19680228?lang=en

• https://orcid.org/0000-0002-3238-813X
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901018432316232
• http://poly-ac.eng.hokudai.ac.jp/bmc
• http://www.eng.hokudai.ac.jp/labo/tre/

• 00271643

• https://orcid.org/0000-0002-3238-813X

• B-1379-2012

• 200901018432316232

• Nanocellulose
• Cellulose
• Sugar beet
• Sugar
• Polysaccharide
• Renewable source
• Biodegradable polymer
• Zero-waste polymer material
• Biomass
• Polymer
• Biopolymer
• Microorganism
• Fermentation engineering
• Biosynthesis mechanism
• Acetic acid bacteria
• Nanomaterial
• Gnetic engineering
• Molecular biology

• Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering), Biofunction and bioprocess engineering
• Life Science, Molecular biology
• Nanotechnology/Materials, Polymer chemistry

• Bachelor's degree program, School of Engineering
• Master's degree program, Graduate School of Chemical Sciences and Engineering
• Doctoral (PhD) degree program, Graduate School of Chemical Sciences and Engineering

Research activity information

• Jul. 2024, The Cellulose Society of Japan, Prize from the Cellulose Society of Japan
  Kenji Tajima
• May 2015, 製糖技術研究会, 第112回製糖技術研究会賞
  田島健次、他
• Jul. 2010, セルロース学会, 林治助賞
  Japan
• Aug. 2004, International Symposium on Bio Polyesters 2004, Poster award
  TAJIMA Kenji
• Sep. 2002, 高分子化学奨励賞<平成１４年度>
  Japan

• Short dextran–solanesol block copolymer as a bio-based platform for multifunctional wormlike micelles
  Weeranuch Lang; Takayuki Kurokawa; Sho Fukushima; Tomohisa Watanabe; Minami Ebe; Redouane Borsali; Feng Li; Takuya Yamamoto; Kenji Tajima; Kenji Takahashi; Toshifumi Satoh; Takuya Isono
  Carbohydrate Polymers, 388, 125523, 125523, Elsevier BV, Sep. 2026, [Peer-reviewed]
  English, Scientific journal
• Three-dimensional cell-culture systems using nanofibrillated bacterial cellulose restores drug metabolism activity in HepG2 hepatocellular carcinoma cells
  Hidenori Ando; Cristina Nana Amorim Matsuo; Shunsuke Akagi; Kenji Tajima; Haruka Takata; Ryo Serizawa; Tokuo Matsushima; Takatomo Kusano; Tatsuhiro Ishida
  Biomedical Materials, 21, 3, 035025, 035025, IOP Publishing, 01 Jun. 2026, [Peer-reviewed]
  English, Scientific journal, Abstract
  
  Three-dimensional (3D) culture more faithfully reproduces in vivo –like cell interactions and functions than conventional two-dimensional (2D) monolayers. Although HepG2 cells are widely used in drug discovery, their 2D cultures exhibit low expression of drug-metabolizing enzymes such as cytochrome P450s (CYPs), limiting utility for toxicity and pharmacokinetic studies. Nano-fibrillated bacterial cellulose (NFBC) is a unique biomaterial that features exceptional homogeneity, high purity, and excellent biocompatibility. We recently employed two NFBC-based 3D culture systems: the Suspension and the OnGel methods. However, the capacity of NFBC to improve hepatocyte-specific functions has yet to be systematically explored. In this study, we aimed to establish a 3D culture platform for HepG2 cells using NFBC to restore hepatic functionality, and drug-metabolizing activity in particular, via comparison with conventional 2D monolayers. Both of these 3D culture systems produced viable HepG2 spheroids with good proliferation. Exploratory microarray profiling suggested broad upregulation of multiple absorption, distribution, metabolism and excretion (ADME) genes in the HepG2 spheroids. Among these, the enzyme Cytochrome P450 3A4 (CYP3A4) showed a significant increase in protein expression and in enzymatic activity in the HepG2 spheroids, which was functionally confirmed by acetaminophen (APAP) toxicity via its bioactivation into toxic metabolite. In addition, the HepG2 spheroids displayed reduced sensitivity to the anticancer drug doxorubicin compared to 2D monolayer. Collectively, these findings demonstrate that NFBC-based 3D culture systems effectively restore liver-specific functions in HepG2 cells, which results in HepG2 spheroids with metabolic competence and physiologically relevant drug responses, and could offer a promising tool for high-throughput in vitro drug screening.
• Physisorption of Cyclic Poly(ethylene glycol) on Platinum Nanoparticles for Dispersion Stabilization and Catalytic Applications
  Mayu Kakizaki; Makoto Hikichi; Kotaro Okawa; Masatoshi Maeki; Manabu Tokeshi; Ryota Suzuki; Tianle Gao; Feng Li; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Takuya Yamamoto
  Colloids and Interfaces, 10, 3, 40, 40, MDPI AG, 12 May 2026, [Peer-reviewed]
  English, Scientific journal, Dispersion stabilization of nanoparticles for catalytic reactions is an important issue. However, dispersing agents should be carefully selected not to hinder catalytic performance. In the present study, physisorption of cyclic poly(ethylene glycol) (c-PEG) onto platinum nanoparticles (PtNPs) was investigated in comparison with unmodified PtNPs (PtNPs/No PEG), PtNPs mixed with linear PEG (PtNPs/HO-PEG-OH), and PtNPs chemisorbed with HS-PEG-OMe (PtNPs/HS-PEG-OMe). DLS showed a significant increase in the particle size for PtNPs/c-PEG and PtNPs/HS-PEG-OMe compared to PtNPs/No PEG and PtNPs/HO-PEG-OH. ζ-potential measurements revealed values around −30 mV for PtNPs/No PEG and PtNPs/HO-PEG-OH, whereas PtNPs/c-PEG and PtNPs/HS-PEG-OMe approached 0 mV, which indicated that c-PEG and HS-PEG-OMe adsorb onto PtNPs to form a shielding layer. Moreover, PtNPs/c-PEG and PtNPs/HS-PEG-OMe were stable in a phosphate-buffered saline (PBS) solution, but PtNPs/No PEG and PtNPs/HO-PEG-OH immediately aggregated. This suggests that high dispersion stability by c-PEG is comparable to ordinary surface modification using HS-PEG-OMe. Furthermore, the catalytic ability of PtNPs/c-PEG and PtNPs/HS-PEG-OMe was compared in various reactions. As a result, physisorbed PtNPs/c-PEG showed suitable catalytic activities, whereas chemisorbed PtNPs/HS-PEG-OMe was significantly hampered by the blocking of the catalytic sites with thiol in some reactions. Thus, physisorption of c-PEG endows PtNPs with dispersion stability and maintains the catalytic ability, leading to an alternative way of modifying metal nanoparticles.
• Biobased High- T _g Aliphatic Polyethers via the Ring-Opening Polymerization of Dihydrolevoglucosenone-Derived Spiro-Epoxides
  Hiroto Ayakawa; Tatsuya Nishimura; Feng Li; Tianle Gao; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Katsuhiro Maeda; Toshifumi Satoh
  ACS Macro Letters, American Chemical Society (ACS), 26 Apr. 2026, [Peer-reviewed]
  English, Scientific journal
• Polymer Brush Morphology-Directed Orientation and Arrangement of Gold Nanorod Arrays
  Yu Sekizawa; Shohei Shiomoto; Takuya Isono; Kenji Tajima; Masaru Tanaka; Toshifumi Satoh; Kuniharu Ijiro; Hideyuki Mitomo
  Chemistry of Materials, American Chemical Society (ACS), 07 Apr. 2026, [Peer-reviewed]
  English, Scientific journal
• Temperature- and Molecular Weight-Dependent Characteristics of Physisorption of Cyclic Poly(ethylene glycol) on Gold Surfaces
  Yuki Maruyama; Tomohisa Watanabe; Tianle Gao; Feng Li; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Hideyuki Mitomo; Takuya Yamamoto
  Langmuir, American Chemical Society (ACS), 06 Apr. 2026, [Peer-reviewed]
  English, Scientific journal
• Synthesis of Deuterated Multicyclic Polystyrenes for a SANS Study to Explore the Structural Characteristics of Multicyclic Architectures in the Bulk
  Yamato Ebii; Tetsuo Deguchi; Kennosuke Takaya; Feng Li; Kenji Tajima; Takuya Yamamoto; Takuya Isono; Toshifumi Satoh
  Macromolecules, American Chemical Society (ACS), 13 Feb. 2026, [Peer-reviewed]
  English, Scientific journal
• Microphase separation in maltooligosaccharide-based block co-oligomers with dominant sugar volume fractions: Toward a comprehensive understanding of the phase behavior
  Chaehun Lee; Taiki Nishimura; Feng Li; Takuya Yamamoto; Redouane Borsali; Kenji Tajima; Hsin-Lung Chen; Toshifumi Satoh; Takuya Isono
  Carbohydrate Polymers, 371, 124488, 124488, Elsevier BV, Jan. 2026, [Peer-reviewed]
  English, Scientific journal
• Self-assembly of dextran-solanesol block copolymers into spherical micelles for photostabilization of quercetin
  Weeranuch Lang; Tomohisa Watanabe; Yurika Fujiwara; Sho Fukushima; Yu-Jen Shao; Redouane Borsali; Kenji Takahashi; Feng Li; Takuya Yamamoto; Kenji Tajima; Toshifumi Satoh; Takuya Isono
  Carbohydrate Polymers, 372, 124508, 124508, Elsevier BV, Jan. 2026, [Peer-reviewed]
  English, Scientific journal
• Molecular Design of POSS–Oligosaccharide Hybrid Materials for Stabilizing Soft-Matter Quasicrystals and Frank–Kasper Phases
  Taiki Nishimura; Chieh-Jen Cheng; Yu Oishi; Ting-Wei Chang; Feng Li; Tianle Gao; Redouane Borsali; Takuya Yamamoto; Kenji Tajima; Hsin-Lung Chen; Toshifumi Satoh; Takuya Isono
  Macromolecules, American Chemical Society (ACS), 30 Dec. 2025, [Peer-reviewed]
  English, Scientific journal
• Ultrasonic Formation of Highly Concentrated Aqueous Dispersion of Fullerene–Polymer Nanoparticles by Charge Transfer
  Yubo Wang; Sota Arima; Toshihiro Shimada; Hiroshi Hirata; Tianle Gao; Feng Li; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin‐ichiro Sato; Takuya Yamamoto
  Macromolecular Rapid Communications, Wiley, 29 Dec. 2025, [Peer-reviewed]
  English, Scientific journal, ABSTRACT
  
  Fullerenes attract much interest due to the potentials to various applications. However, their insolubility in water and some organic solvents often hinders development. Herein, poly(ethylene glycol) (PEG), polyvinylpyrrolidone (PVP), poly(vinyl alcohol) (PVA), polyacrylic acid (PAA), and Pluronic L64 and 17R4, are attempted for dispersing fullerene in water via ultrasonication, and some of these are found to disperse an exceedingly large amount. The maximum concentration of C ₆₀ is achieved with Pluronic L64, being 9.8 g/L (13.6 m m ) without re‐aggregation for a long time. Electron paramagnetic resonance (EPR) spectroscopy shows a significant amount of radical species existing in the fullerene–polymer complexes, which are stable for several weeks. DLS and TEM exhibit the formation of nanoparticles, and NMR, FT‐IR, and MALDI‐TOF MS are used to characterize the fullerene nanoparticles–polymer complexes. The aqueous dispersions of the complexes can be dried and redispersed in water and polar organic solvents. Column chromatographic separation is performed to give unreacted fullerene and fullerene–polymer complexes, the latter of which shows a strong EPR signal. Density functional simulations reveal partial electron transfer from the PEG segment to fullerene, which causes charge separation in the complexes, resulting in the excellent dispersibility in water, while the poly(propylene glycol) (PPG) segment likely assists the complexation by hydrophobic interactions with fullerene.
• Structure–Property Relationships in Multicyclic Polystyrenes: Synthesis, Physical Properties, and Comparison with Acyclic Counterparts
  Yamato Ebii; Tomohisa Watanabe; Feng Li; Kenji Tajima; Takuya Yamamoto; Takuya Isono; Toshifumi Satoh
  Macromolecules, 58, 20, 11258, 11269, American Chemical Society (ACS), 16 Oct. 2025, [Peer-reviewed]
  English, Scientific journal
• Nanoscale dispersion of maltooligosaccharides in poly(ε-caprolactone) for an enhanced mechanical performance and marine-biodegradability characteristics
  Yurika Fujiwara; Wilasinee Kotcharoen; Takaya Kobayashi; Yuki Tsuji; Kazushige Suzuki; Lang Weeranuch; Feng Li; Takuya Yamamoto; Yutaka Takeuchi; Kenji Takahashi; Redouane Borsali; Kenji Tajima; Toshifumi Satoh; Takuya Isono
  Polymer Degradation and Stability, 111663, 111663, Elsevier BV, Sep. 2025, [Peer-reviewed]
  English, Scientific journal
• Modulation of gluconic acid metabolism enhances nanofibrillated bacterial cellulose production from agro-industrial by-products
  Ryo Takahama; Go Takayama; Miho Suginaka; Yuma Ishido; Shunsuke Nagai; Kazuki Nagai; Masato Uenishi; Kenji Tajima
  openRxiv, 30 Aug. 2025, [Last author, Corresponding author]
  English, Abstract
  
  Efficient and robust bacterial cellulose production is essential for advancing the sustainable bioeconomy. In this study, we investigated the impacts of metabolism of organic acids, mainly gluconic acid (GA), on nanofibrillated bacterial cellulose (NFBC) production by Komagataeibacter intermedius NEDO-01 under various culture conditions in aerated stirred-tank reactors. In cultures of the wild-type strain in a standard medium, rapid GA production decreased the medium pH and depleted glucose, inhibiting cell growth and reducing the NFBC yield. However, proper pH control and continuous feeding reversed these effects, resulting in a 3-fold increase in NFBC yield (from 2.45 to 7.59 g/L). In cultures of a glucose dehydrogenase gene-deficient (Δ gcd ) strain, lack of a pH drop and glucose depletion facilitated better cell growth, yielding 1.85-times more NFBC than that in wild-type cultures under pH-uncontrolled no-feed conditions (4.53 g/L). Notably, GA supplementation accelerated cell growth but significantly inhibited NFBC synthesis, suggesting that GA uptake redirects the carbon flux toward central metabolism.
  
  In the corn steep liquor (Csl)-based medium, cell growth was significantly enhanced, and NFBC yield was equivalent to or higher than that obtained with the Hestrin–Shramm medium. GA accumulation was markedly reduced, suppressing pH fluctuation. Under these optimized conditions, three molasses types were tested with Csl, yielding relatively high NFBC. Structural analysis of NFBC produced using these alternative media revealed slight differences in the fiber width distribution, with crystallinity and fiber width remaining constant. Overall, NFBC of consistent quality can be produced in stirred-tank reactors using Komagataeibacter spp. from various agricultural by-products.
  
  Importance
  
  In this study, we investigated the interplay between organic acid metabolism and nanofibrillated bacterial cellulose (NFBC) production in stirred-tank reactor (STR) cultures of Komagataeibacter intermedius NEDO-01. While it is well known that gluconic acid production competes with cellulose biosynthesis in Komagataeibacter , the quantitative relationship between these pathways under varying culture conditions has not been fully elucidated. By applying optimized feeding strategies and employing a glucose dehydrogenase knockout mutant, we demonstrated that suppressing gluconic acid accumulation significantly enhances NFBC yield. Furthermore, we explored the use of agro-industrial by-products, including molasses and corn steep liquor, as alternative, low-cost feedstocks. Structural characterization confirmed that NFBCs produced under these conditions maintained consistent quality. These findings contribute to the development of scalable, cost-effective microbial production processes for nanocellulose, which is essential for advancing the sustainable bioeconomy.
  
  Key Points
  
  GA accumulation inhibited growth and cellulose production by Komagataeibacter
  
  Enhanced central metabolism elevated NFBC yield but reduced its production rate
  
  Consistent NFBC properties were achieved in STRs using various by-product sources
• POSS–Oligosaccharide Hybrid Materials as a Versatile Platform for Constructing Unique Spherical Phases
  Taiki Nishimura; Chieh-Jen Cheng; Yu Oishi; Feng Li; Redouane Borsali; Takuya Yamamoto; Kenji Tajima; Hsin-Lung Chen; Toshifumi Satoh; Takuya Isono
  Macromolecules, American Chemical Society (ACS), 10 Jul. 2025, [Peer-reviewed]
  English, Scientific journal
• Conjugated Polymer Nanoparticles and Thin Films of Defect-Free Cyclic P3HT: Effects of Polymer Topology on the Nanostructure
  Tomohisa Watanabe; Masatoshi Maeki; Manabu Tokeshi; Tianle Gao; Feng Li; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Takuya Yamamoto
  Molecules, 30, 12, 2490, 2490, MDPI AG, 06 Jun. 2025, [Peer-reviewed]
  English, Scientific journal, Conjugated polymer nanoparticles (CP NPs) attract attention as nanoscale materials used for a variety of applications. In relation to this, the internal structure of CP NPs is an important factor for their properties, and numerous investigations have been carried out to control their nanomorphology. Here, we report the formation of CP NPs from defect-free cyclic poly(3-hexylthiophene) (c-P3HT) using a microfluidic device, and the effect of polymer topology on their structural and solvatochromic properties was investigated. CP NPs from c-P3HT exhibited reduced particle sizes and hypsochromic shifts in the absorption spectrum when compared to CP NPs obtained from corresponding linear P3HT (l-P3HT). Furthermore, steady responses in the solvatochromism of CP NPs from c-P3HT were observed, while those from l-P3HT displayed molecular weight dependency. These topology effects were caused by the change in the conjugation length, solubility, and crystallinity upon cyclization. Grazing incidence X-ray scattering (GIXS) studies of spin-coated P3HT films further showed a reduced interchain order and a larger proportion of face-on molecular orientation on a substrate for c-P3HTs. The various distinct structures observed for c-P3HT indicate the use of polymer topology as a means of nanostructure regulation.
• Unique Complex Structure and Mechanism of Physisorption of Defect-Free Cyclic Poly(ethylene glycol) onto Gold Nanoparticles
  Tomohisa Watanabe; Masato Yamasaki; Tsuyoshi Nakai; Bincy Akkoli Pottammal; Yuki Maruyama; Hiroki Iwase; Akiyuki Ryoki; Tianle Gao; Feng Li; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Takuya Yamamoto
  Langmuir, American Chemical Society (ACS), 26 May 2025, [Peer-reviewed]
  English, Scientific journal
• Morphological details of cellulose nanofibers and their composites for Raman sensing application
  Brian J. Ree; Zi Jia Low; Kuan Hoon Ngoi; Jia Chyi Wong; Kyeong Sik Jin; Chin Hua Chia; Kenji Tajima
  International Journal of Biological Macromolecules, 142658, 142658, Elsevier BV, Apr. 2025, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, 34012607
• A 3D Cell-Culture System That Uses Nano-Fibrillated Bacterial Cellulose to Prepare a Spherical Formulation of Culture Cells
  Shunsuke Akagi; Hidenori Ando; Cristina Nana Amorim Matsuo; Kenji Tajima; Haruka Takata; Tokuo Matsushima; Takatomo Kusano; Tatsuhiro Ishida
  Biological and Pharmaceutical Bulletin, 48, 1, 23, 32, Pharmaceutical Society of Japan, 24 Jan. 2025, [Peer-reviewed]
  English, Scientific journal
• Synthesis of polycarbonates and polyesters via tetraalkylammonium salt-catalyzed ring-opening polymerization
  Ryota Suzuki; Satoru Takagi; Minori Matsuda; Takuya Yamamoto; Kenji Tajima; Feng Li; Takuya Isono; Toshifumi Satoh
  Chemistry Letters, 54, 1, Oxford University Press (OUP), 08 Jan. 2025, [Peer-reviewed]
  English, Scientific journal, Abstract
  
  Tetraalkylammonium salt was found to be an effective catalyst for the ring-opening polymerization of cyclic carbonates and cyclic esters, yielding aliphatic polycarbonates and polyesters with narrow dispersities. An important feature of this catalytic system is its tunable catalytic ability, which was achieved by altering the alkyl substrates and counter anions. The catalytic mechanism was studied by 1H NMR spectroscopy.
• Molecular Design of a Discrete Oligosaccharide-block-Oligodimethylsiloxane System: Toward Microphase Separation with 1 nm Domain Size and Angstrom-Scale Size Control
  Taiki Nishimura; Chaehun Lee; Ryosuke Nunokawa; Yu-Hung Cheng; Feng Li; Takuya Yamamoto; Kenji Tajima; Redouane Borsali; Hsin-Lung Chen; Toshifumi Satoh; Takuya Isono
  Macromolecules, American Chemical Society (ACS), 18 Dec. 2024, [Peer-reviewed]
  English, Scientific journal
• Self-assembly of malto-oligosaccharide-block-solanesol in aqueous solutions: Investigating morphology and sugar-based physiological compatibility
  Weeranuch Lang; Tomohisa Watanabe; Chaehun Lee; Sho Fukushima; Feng Li; Takuya Yamamoto; Kenji Tajima; Takayoshi Tagami; Redouane Borsali; Kenji Takahashi; Toshifumi Satoh; Takuya Isono
  Carbohydrate Polymers, 123207, 123207, Elsevier BV, Dec. 2024
  Scientific journal
• Fully biosourced amphiphilic block copolymer from tamarind seed xyloglucan and solanesol: synthesis, aqueous self-assembly, and drug encapsulation
  Weeranuch Lang; Tomohisa Watanabe; Chaehun Lee; Takayoshi Tagami; Feng Li; Takuya Yamamoto; Kenji Tajima; Redouane Borsali; Kenji Takahashi; Toshifumi Satoh; Takuya Isono
  Carbohydrate Polymers, 123181, 123181, Elsevier BV, Dec. 2024, [Peer-reviewed]
  English, Scientific journal
• Molecular structure of enzyme-synthesized amylose-like chimeric isomaltomegalosaccharides and their encapsulation of the sulfasalazine prodrug
  Weeranuch Lang; Yoshiaki Yuguchi; Chun-Yao Ke; Ting-Wei Chang; Yuya Kumagai; Wilaiwan Kaenying; Takayoshi Tagami; Feng Li; Takuya Yamamoto; Kenji Tajima; Kenji Takahashi; Takuya Isono; Toshifumi Satoh; Atsuo Kimura
  Carbohydrate Polymers, 122956, 122956, Elsevier BV, Nov. 2024, [Peer-reviewed]
  English, Scientific journal
• Application of bacterial-derived long cellulose nanofiber to suspension culture of mammalian cells as a shear protectant
  Eiichiro Kaneko; Haruto Tsujisaki; Masashi Fujiwara; Hidenori Ando; Yasushi Sato; Tatsuhiro Ishida; Hirofumi Tani; Kenji Tajima
  International Journal of Biological Macromolecules, 280, 135938, 135938, Elsevier BV, Nov. 2024, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Poly(butylene succinate) reinforced by small amount of grafted nanofibrillated bacterial cellulose: Toughness variability based on nanocomposites preparation method
  Hamidah binti Hashim; Xiaochao Xia; Hiroshi Kani; Shuichiro Seno; Feng Li; Takuya Isono; Takuya Yamamoto; Hirofumi Tani; Toshifumi Satoh; Kenji Tajima
  Composites Part A: Applied Science and Manufacturing, 185, 108341, 108341, Elsevier BV, Oct. 2024, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Polyester Adhesives via One-Pot, One-Step Copolymerization of Cyclic Anhydride, Epoxide, and Lactide
  Ryota Suzuki; Toshiki Miwa; Ryosuke Nunokawa; Ayaka Sumi; Masaru Ando; Katsuaki Takahashi; Akira Takagi; Takuya Yamamoto; Kenji Tajima; Feng Li; Takuya Isono; Toshifumi Satoh
  Polymers, 16, 19, 2767, 2767, MDPI AG, 30 Sep. 2024, [Peer-reviewed]
  English, Scientific journal, Polyesters (PEs) are sustainable alternatives for conventional polymers owing to their potential degradability, recyclability, and the wide availability of bio-based monomers for their synthesis. Herein, we used a one-pot, one-step self-switchable polymerization linking the ring-opening alternating copolymerization (ROAC) of epoxides/cyclic anhydrides with the ring-opening polymerization (ROP) of L-lactide (LLA) to synthesize PE-based hot-melt adhesives with a high bio-based content. In the cesium pivalate-catalyzed self-switchable polymerization of glutaric anhydride (GA), butylene oxide (BO), and LLA using a diol initiator, the ROAC of GA and BO proceeded whereas the ROP of LLA simultaneously proceeded very slowly, resulting in a copolyester consisting of poly(GA-alt-BO) and poly(L-lactide) (PLLA) segments with tapered regions, that is, PLLA-tapered block-poly(GA-alt-BO)-tapered block-PLLA (PLLA-tb-poly(GA-alt-BO)-tb-PLLA). Additionally, a series of tapered-block or real-block copolyesters consisting of poly(anhydride-alt-epoxide) (A segment) and PLLA (B segment) with AB-, BAB-, (AB)3-, and (AB)4-type architectures of different compositions and molecular weights were synthesized by varying the monomer combinations, alcohol initiators, and initial feed ratios. The lap shear tests of these copolyesters revealed an excellent relationship between the adhesive strength and polymer structural parameters. The (AB)4-type star-block copolyester (poly(GA-alt-BO)-tb-PLLA)4 exhibited the best adhesive strength (6.74 ± 0.64 MPa), comparable to that of commercial products, such as PE-based and poly(vinyl acetate)-based hot-melt adhesives.
• Detailed structural analyses and viscoelastic properties of nano-fibrillated bacterial celluloses
  Haruto Tsujisaki; Masaaki Hosokawa; Yuichi Takasaki; Yoshifumi Yamagata; Yui Kawabata; Daisuke Tatsumi; Shuichiro Seno; Keisuke Miyamoto; Takuya Isono; Takuya Yamamoto; Hirofumi Tani; Toshifumi Satoh; Hiroshi Orihara; Kenji Tajima
  Carbohydrate Polymer Technologies and Applications, 100565, 100565, Elsevier BV, Sep. 2024, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, 34012607
• Bacterial Cellulose Production in Space
  Akiko TANAKA; Tomoya IMAI; Misako KOGA; Naoki SUNAGAWA; Kiyohiko IGARASHI; Kenji TAJIMA; Hiroaki TANAKA
  International journal of microgravity science and application, 41, 3, 410302, 31 Jul. 2024, [Peer-reviewed]
  English, Scientific journal, 34012600
• Optimizing crystal transitions in low-temperature, low-concentration NaOH solutions to prepare cellulose I and II composite materials
  Yuki Kugo; Takuya Isono; Masashi Fujiwara; Toshifumi Sato; Hirofumi Tani; Tomoki Erata; Kenji Tajima
  Polymer Journal, Springer Science and Business Media LLC, 12 Jun. 2024, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Elucidating the structural changes of cellulose molecules and dynamics of Na ions during the crystal transition from cellulose I to II in low temperature and low concentration NaOH solution
  Yuki Kugo; Satoshi Nomura; Takuya Isono; Shin-ichiro Sato; Masashi Fujiwara; Toshifumi Satoh; Hirofumi Tani; Tomoki Erata; Kenji Tajima
  Carbohydrate Polymers, 332, 121907, 121907, Elsevier BV, May 2024, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Organobase-Catalyzed Ring-Opening Copolymerization of Cyclic Anhydrides and Oxetanes: Establishment and Application in Block Copolymer Synthesis
  Iyoka Ota; Ryota Suzuki; Yuta Mizukami; Xiaochao Xia; Kenji Tajima; Takuya Yamamoto; Feng Li; Takuya Isono; Toshifumi Satoh
  Macromolecules, American Chemical Society (ACS), 11 Apr. 2024, [Peer-reviewed]
  English, Scientific journal
• Ultrasmall 3D network morphologies from biobased sugar–terpenoid hybrid block co-oligomers in the bulk and the thin film states
  Chaehun Lee; Brian J. Ree; Kai Chen; Ryoya Komaki; Satoshi Katsuhara; Takuya Yamamoto; Redouane Borsali; Kenji Tajima; Hsin-Lung Chen; Toshifumi Satoh; Takuya Isono
  Giant, 17, 100211, 100211, Elsevier BV, Mar. 2024, [Peer-reviewed]
  English, Scientific journal
• Acetyl Cellooligosaccharide-Based Block Copolymers for Toughening Cellulose Triacetate/Poly(ε-caprolactone) Biodegradable Blends
  Satoshi Katsuhara; Yuki Tsuji; Naoki Sunagawa; Kiyohiko Igarashi; Kenji Takahashi; Takuya Yamamoto; Feng Li; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  ACS Sustainable Chemistry & Engineering, American Chemical Society (ACS), 14 Feb. 2024, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Crystallinity improvements in cellulose II after multiple posttreatment cycles with a dilute NaOH solution
  Yuki Kugo; Satoshi Nomura; Takuya Isono; Masashi Fujiwara; Toshifumi Satoh; Hirofumi Tani; Tomoki Erata; Kenji Tajima
  Polymer Journal, Springer Science and Business Media LLC, 13 Feb. 2024, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Chemically Recyclable Unnatural (1→6)-Polysaccharides from Cellulose-Derived Levoglucosenone and Dihydrolevoglucosenone
  Yuta Mizukami; Yuto Kakehi; Feng Li; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  ACS Macro Letters, 252, 259, American Chemical Society (ACS), 09 Feb. 2024, [Peer-reviewed]
  English, Scientific journal
• Synthesis of Amino-Functionalized Polyester via Ring-Opening Alternating Copolymerization of Glycidylamines with Cyclic Anhydrides
  Ryota Suzuki; Tianle Gao; Ayaka Sumi; Takuya Yamamoto; Kenji Tajima; Feng Li; Takuya Isono; Toshifumi Satoh
  Polymer Chemistry, Royal Society of Chemistry (RSC), 2024
  English, Scientific journal, Amino-functionalized polyesters (APEs) are a remarkable class of polymeric materials with a wide range of applications, e.g. as antibacterial materials, gene delivery carriers, and biodegradable plastics. However, the current APE...
• Rotaxane Formation of Multicyclic Polydimethylsiloxane in a Silicone Network: A Step toward Constructing “Macro-Rotaxanes” from High-Molecular-Weight Axle and Wheel Components
  Minami Ebe; Asuka Soga; Kaiyu Fujiwara; Brian J. Ree; Hironori Marubayashi; Katsumi Hagita; Atsushi Imasaki; Miru Baba; Takuya Yamamoto; Kenji Tajima; Tetsuo Deguchi; Hiroshi Jinnai; Takuya Isono; Toshifumi Satoh
  Angewandte Chemie International Edition, 135, 35, e202304493, Wiley, Jul. 2023, [Peer-reviewed]
  English, Scientific journal, Abstract
  
  Rotaxanes consisting of a high‐molecular‐weight axle and wheel components (macro‐rotaxanes) have high structural freedom, and are attractive for soft‐material applications. However, their synthesis remains underexplored. Here, we investigated macro‐rotaxane formation by the topological trapping of multicyclic polydimethylsiloxanes (mc‐PDMSs) in silicone networks. mc‐PDMS with different numbers of cyclic units and ring sizes was synthesized by cyclopolymerization of a α,ω‐norbornenyl‐functionalized PDMS. Silicone networks were prepared in the presence of 10–60 wt % mc‐PDMS, and the trapping efficiency of mc‐PDMS was determined. In contrast to monocyclic PDMS, mc‐PDMSs with more cyclic units and larger ring sizes can be quantitatively trapped in the network as macro‐rotaxanes. The damping performance of a 60 wt % mc‐PDMS‐blended silicone network was evaluated, revealing a higher tan δ value than the bare PDMS network. Thus, macro‐rotaxanes are promising as non‐leaching additives for network polymers.
• Effect of degree of substitution on the microphase separation and mechanical properties of cellooligosaccharide acetate-based elastomers
  Katsuhara, S.; Sunagawa, N.; Igarashi, K.; Takeuchi, Y.; Takahashi, K.; Yamamoto, T.; Li, F.; Tajima, K.; Isono, T.; Satoh, T.
  Carbohydrate Polymers, 316, 120976, 120976, Elsevier {BV}, May 2023, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Bacterial Cellulose Nanofibers Modified with Quaternary Ammonium Salts for Antimicrobial Applications
  Hiroyuki Kono; Yoichiro Sogame; Uugan-Erdene Purevdorj; Makoto Ogata; Kenji Tajima
  ACS Applied Nano Materials, 6, 6, 4854, 4863, American Chemical Society (ACS), 02 Mar. 2023, [Peer-reviewed], [Last author]
  English, Scientific journal
• Molecular Weight-Dependent Oxidation and Optoelectronic Properties of Defect-Free Macrocyclic Poly(3-hexylthiophene)
  Ryohei Sato; Atsuo Utagawa; Koji Fushimi; Feng Li; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Hiroshi Hirata; Yoshihiro Kikkawa; Takuya Yamamoto
  Polymers, 15, 3, 666, 666, MDPI AG, 28 Jan. 2023, [Peer-reviewed]
  English, Scientific journal, The redox behaviors of macrocyclic molecules with an entirely π-conjugated system are of interest due to their unique optical, electronic, and magnetic properties. In this study, defect-free cyclic P3HT with a degree of polymerization (DPn) from 14 to 43 was synthesized based on our previously established method, and its unique redox behaviors arising from the cyclic topology were investigated. Cyclic voltammetry (CV) showed that the HOMO level of cyclic P3HT decreases from –4.86 eV (14 mer) to –4.89 eV (43 mer), in contrast to the linear counterparts increasing from –4.94 eV (14 mer) to –4.91 eV (43 mer). During the CV measurement, linear P3HT suffered from electro-oxidation at the chain ends, while cyclic P3HT was stable. ESR and UV–Vis–NIR spectroscopy suggested that cyclic P3HT has stronger dicationic properties due to the interactions between the polarons. On the other hand, linear P3HT showed characteristics of polaron pairs with multiple isolated polarons. Moreover, the dicationic properties of cyclic P3HT were more pronounced for the smaller macrocycles.
• Installation of the adamantyl group in polystyrene-block-poly(methyl methacrylate) via Friedel–Crafts alkylation to modulate the microphase-separated morphology and dimensions
  Takuya Isono; Ema Baba; Shunma Tanaka; Ken Miyagi; Takahiro Dazai; Feng Li; Takuya Yamamoto; Kenji Tajima; Toshifumi Satoh
  Polymer Chemistry, 14, 22, 2675, 2684, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed]
  English, Scientific journal, We present the polystyrene block post-polymerization modification of PS-b-PMMA through Friedel–Crafts alkylation with adamantanols to modulate its microphase-separated morphology and dimensions.
• Cyclopolymerization: A versatile approach to multicyclic polystyrene and polystyrene-containing multicyclic copolymer
  Yamato Ebii; Yoshinobu Mato; Feng Li; Kenji Tajima; Takuya Yamamoto; Takuya Isono; Toshifumi Satoh
  Polymer Chemistry, 14, 26, 3099, 3109, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed]
  Scientific journal, Macromolecules with cyclic topologies have attracted significant attention because of their unique structures. Herein, we report the systematic synthesis of multicyclic polystyrene (mc-PS) via cyclopolymerization of α,ω-dinorbornenyl end-functionalized PS macromonomers...
• Size Control and Enhanced Stability of Silver Nanoparticles by Cyclic Poly(ethylene glycol)
  Wang, Y.; Quinsaat, J.E.Q.; Li, F.; Isono, T.; Tajima, K.; Satoh, T.; Sato, S.-I.; Yamamoto, T.
  Polymers, 14, 21, 4535, 4535, MDPI AG, 26 Oct. 2022, [Peer-reviewed]
  English, Scientific journal
• Polyether/Polythioether Synthesis via Ring-Opening Polymerization of Epoxides and Episulfides Catalyzed by Alkali Metal Carboxylates
  Tianle Gao; Xiaochao Xia; Kenji Tajima; Takuya Yamamoto; Takuya Isono; Toshifumi Satoh
  Macromolecules, 55, 21, 9373, 9383, American Chemical Society (ACS), 21 Oct. 2022, [Peer-reviewed]
  English, Scientific journal
• Tailored Solid-State Carbohydrate Nanostructures Based on Star-Shaped Discrete Block Co-Oligomers
  Takuya Isono; Ryoya Komaki; Nao Kawakami; Kai Chen; Hsin-Lung Chen; Chaehun Lee; Kazushige Suzuki; Brian J. Ree; Hiroaki Mamiya; Takuya Yamamoto; Redouane Borsali; Kenji Tajima; Toshifumi Satoh
  Biomacromolecules, 23, 9, 3978, 3989, American Chemical Society (ACS), 12 Sep. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Carbohydrates are key building blocks for advanced functional materials owing to their biological functions and unique material properties. Here, we propose a star-shaped discrete block co-oligomer (BCO) platform to access carbohydrate nanostructures in bulk and thin-film states via the microphase separation of immiscible carbohydrate and hydrophobic blocks (maltooligosaccharides with 1-4 glucose units and solanesol, respectively). BCOs with various star-shaped architectures and saccharide volume fractions were synthesized using a modular approach. In the bulk, the BCOs self-assembled into common lamellar, cylindrical, and spherical carbohydrate microdomains as well as double gyroid, hexagonally perforated lamellar, and Fddd network morphologies with domain spacings of ∼7 nm. In thin films, long-range-ordered periodic carbohydrate microdomains were fabricated via spin coating. Such controlled spatial arrangements of functional carbohydrate moieties on the nanoscale have great application potential in biomedical and nanofabrication fields.
• Synthesis of hyperbranched polyesters via the ring-opening alternating copolymerisation of epoxides with a cyclic anhydride having a carboxyl group
  Ryota Suzuki; Xiaochao Xia; Tianle Gao; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Polymer Chemistry, 13, 38, 5469, 5477, Royal Society of Chemistry (RSC), Aug. 2022, [Peer-reviewed]
  English, Scientific journal, Hyperbranched polyesters (HBPEs) are well-known interesting materials used in many fields.
• Improving the mechanical properties of polycaprolactone using functionalized nanofibrillated bacterial cellulose with high dispersibility and long fiber length as a reinforcement material
  Hashim, H.B.; Emran, N.A.A.B.; Isono, T.; Katsuhara, S.; Ninoyu, H.; Matsushima, T.; Yamamoto, T.; Borsali, R.; Satoh, T.; Tajima, K.
  Composites Part A: Applied Science and Manufacturing, 158, 106978, 106978, Elsevier {BV}, May 2022, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, 34012607
• Fabrication of Ultrafine, Highly Ordered Nanostructures Using Carbohydrate-Inorganic Hybrid Block Copolymers
  Taiki Nishimura; Satoshi Katsuhara; Chaehun Lee; Brian Ree; Redouane Borsali; Takuya Yamamoto; Kenji Tajima; Toshifumi Satoh; Takuya Isono
  Nanomaterials, 12, 10, 1653, 1653, MDPI AG, May 2022, [Peer-reviewed]
  Scientific journal, Block copolymers (BCPs) have garnered considerable interest due to their ability to form microphase-separated structures suitable for nanofabrication. For these applications, it is critical to achieve both sufficient etch selectivity and a small domain size. To meet both requirements concurrently, we propose the use of oligosaccharide and oligodimethylsiloxane as hydrophilic and etch-resistant hydrophobic inorganic blocks, respectively, to build up a novel BCP system, i.e., carbohydrate-inorganic hybrid BCP. The carbohydrate-inorganic hybrid BCPs were synthesized via a click reaction between oligodimethylsiloxane with an azido group at each chain end and propargyl-functionalized maltooligosaccharide (consisting of one, two, and three glucose units). In the bulk state, small-angle X-ray scattering revealed that these BCPs microphase separated into gyroid, asymmetric lamellar, and symmetric lamellar structures with domain-spacing ranging from 5.0 to 5.9 nm depending on the volume fraction. Additionally, we investigated microphase-separated structures in the thin film state and discovered that the BCP with the most asymmetric composition formed an ultrafine and highly oriented gyroid structure as well as in the bulk state. After reactive ion etching, the gyroid thin film was transformed into a nanoporous-structured gyroid SiO2 material, demonstrating the material’s promising potential as nanotemplates.
• Topology and Sequence-Dependent Micellization and Phase Separation of Pluronic L35, L64, 10R5, and 17R4: Effects of Cyclization and the Chain Ends
  Tomohisa Watanabe; Yubo Wang; Tomoko Ono; Satoru Chimura; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Daichi Ida; Takuya Yamamoto
  Polymers, 14, 9, 1823, 1823, MDPI AG, 29 Apr. 2022, [Peer-reviewed]
  English, Scientific journal, The topology effects of cyclization on thermal phase transition behaviors were investigated for a series of amphiphilic Pluronic copolymers of both hydrophilic–hydrophobic–hydrophilic and hydrophobic–hydrophilic–hydrophobic block sequences. The dye solubilization measurements revealed the lowered critical micelle temperatures (TCMT) along with the decreased micellization enthalpy (ΔHmic) and entropy (ΔSmic) for the cyclized species. Furthermore, the transmittance and dynamic light scattering (DLS) measurements indicated a block sequence-dependent effect on the clouding phenomena, where a profound decrease in cloud point (Tc) was only found for the copolymers with a hydrophilic–hydrophobic–hydrophilic block sequence. Thus, the effect of cyclization on these critical temperatures was manifested differently depending on its block sequence. Finally, a comparison of the linear hydroxy-terminated, methoxy-terminated, and cyclized species indicated the effect of cyclization to be unique from a simple elimination of the terminal hydrophilic moieties.
• Physical characteristics and cell-adhesive properties of in vivo fabricated bacterial cellulose/hyaluronan nanocomposites
  Takahama, R.; Kato, H.; Takayama, G.; Tajima, K.; Kondo, T.
  Cellulose, 29, 6, 3239, 3251, Springer Science and Business Media {LLC}, 07 Mar. 2022, [Peer-reviewed]
  English, Scientific journal
• Reinforcing Poly (methyl methacrylate) with Bacterial Cellulose Nanofibers Chemically Modified with Methacryolyl Groups
  Kono, H.; Tsujisaki, H.; Tajima, K.
  Nanomaterials, 12, 3, 537, 537, MDPI AG, 04 Feb. 2022, [Peer-reviewed], [Last author]
  Scientific journal
• Structural snapshot of a glycoside hydrolase family 8 endo-β-1,4-glucanase capturing the state after cleavage of the scissile bond
  Takaaki Fujiwara; Ayumi Fujishima; Yui Nakamura; Kenji Tajima; Min Yao
  Acta Crystallographica Section D Structural Biology, 78, 2, 228, 237, International Union of Crystallography (IUCr), 01 Feb. 2022, [Peer-reviewed]
  English, Scientific journal, Bacterial cellulose (BC), which is produced by bacteria, is a biodegradable and biocompatible natural resource. Because of its remarkable physicochemical properties, BC has attracted attention for the development and manufacture of biomedical and industrial materials. In the BC production system, the enzyme endo-β-1,4-glucanase, which belongs to glycoside hydrolase family 8 (GH8), acts as a cleaner by trimming disordered cellulose fibers to produce high-quality BC. Understanding the molecular mechanism of the endo-β-1,4-glucanase would help in developing a reasonable biosynthesis of BC. Nevertheless, all of the steps in the reaction of this endo-β-1,4-glucanase are not clear. This study confirms the BC hydrolytic activity of the endo-β-1,4-glucanase from the BC-producing bacterium Enterobacter sp. CJF-002 (EbBcsZ) and reports crystal structures of EbBcsZ. Unlike in previously reported GH8 endo-β-1,4-glucanase structures, here the base catalyst was mutated (D242A) and the structure of this mutant bound to cellooligosaccharide [EbBcsZ(D242A)_CPT] was analyzed. The EbBcsZ(D242A)_CPT structure showed two cellooligosaccharides individually bound to the plus and minus subsites of EbBcsZ. The glucosyl unit in subsite −1 presented a distorted ⁵
  S
  ₁ conformation, a novel snapshot of a state immediately after scissile-bond cleavage. In combination with previous studies, the reaction process of endo-β-1,4-glucanase is described and the β-1,4-glucan-trimming mechanism of EbBcsZ is proposed. The EbBcsZ(D242A)_CPT structure also showed an additional β-1,4-glucan binding site on the EbBcsZ surface, which may help to accept the substrate.
• PEGylation of silver nanoparticles by physisorption of cyclic poly(ethylene glycol) for enhanced dispersion stability, antimicrobial activity, and cytotoxicity
  Onyinyechukwu Justina Oziri; Yubo Wang; Tomohisa Watanabe; Shuya Uno; Masatoshi Maeki; Manabu Tokeshi; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Yutaka Miura; Takuya Yamamoto
  Nanoscale Advances, 4, 2, 532, 545, Royal Society of Chemistry (RSC), 2022, [Peer-reviewed]
  English, Scientific journal, Cyclic PEG without any chemical inhomogeneity was physiosorbed onto AgNPs to PEGylate and drastically enhance the dispersion stability against a physiological condition, white light, and high temperature, while its linear counterparts could not.
• Cellulose-synthesizing machinery in bacteria
  Tajima, K.; Imai, T.; Yui, T.; Yao, M.; Saxena, I.
  Cellulose, 29, 5, 2755, 2777, Springer Science and Business Media {LLC}, 2022, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal, 34000719;34000718
• Topology-Dependent Interaction of Cyclic Poly(ethylene glycol) Complexed with Gold Nanoparticles against Bovine Serum Albumin for a Colorimetric Change
  Onyinyechukwu Justina Oziri; Masatoshi Maeki; Manabu Tokeshi; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Takuya Yamamoto
  Langmuir, 38, 17, 5286, 5295, American Chemical Society (ACS), 08 Dec. 2021, [Peer-reviewed], [International Magazine]
  Japanese, Scientific journal, Unique physical and chemical properties arising from a polymer topology recently draw significant attention. In this study, cyclic poly(ethylene glycol) (c-PEG) was found to significantly interact with bovine serum albumin (BSA), suggested by nuclear magnetic resonance, dynamic light scattering, and fluorescence spectroscopy. On the other hand, linear HO-PEG-OH and MeO-PEG-OMe showed no affinity. Furthermore, a complex of gold nanoparticles and c-PEG (AuNPs/c-PEG) attracted BSA to form aggregates, and the red color of the AuNPs dispersion evidently disappeared, whereas ones with linear PEG or without PEG did not demonstrate such a phenomenon. The interactions among BSA, AuNPs, and PEG were investigated by changing the incubation time and concentration of the components by using UV-Vis and fluorescence spectroscopy.
• Facile Post-Carboxymethylation of Cellulose Nanofiber Surfaces for Enhanced Water Dispersibility
  Hiroyuki Kono; Eiki Tsukamoto; Kenji Tajima
  ACS Omega, 6, 49, 34107, 34114, American Chemical Society (ACS), 01 Dec. 2021, [Peer-reviewed], [Last author]
  English, Scientific journal
• Suzuki–Miyaura Catalyst-Transfer Polycondensation of Triolborate-Type Carbazole Monomers
  Saburo Kobayashi; Mayoh Ashiya; Takuya Yamamoto; Kenji Tajima; Yasunori Yamamoto; Takuya Isono; Toshifumi Satoh
  Polymers, 13, 23, 4168, 4168, MDPI AG, 28 Nov. 2021, [Peer-reviewed]
  English, Scientific journal
• Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter
  Ryo Takahama; Honami Kato; Kenji Tajima; Satomi Tagawa; Tetsuo Kondo
  Biomacromolecules, 22, 11, 4709, 4719, American Chemical Society (ACS), 08 Nov. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms. Their hierarchically regulated production systems show potential for designing nanocomposite fabrication using engineered microorganisms. This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC). The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis. Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii. The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites. This genetic engineering approach facilitates the use of G. hansenii for designing integrated cellulose-based nanomaterials.
• Densely Arrayed Cage-Shaped Polymer Topologies Synthesized via Cyclopolymerization of Star-Shaped Macromonomers
  Yoshinobu Mato; Maho Sudo; Hironori Marubayashi; Brian J. Ree; Kenji Tajima; Takuya Yamamoto; Hiroshi Jinnai; Takuya Isono; Toshifumi Satoh
  Macromolecules, 54, 19, 9079, 9090, American Chemical Society ({ACS}), 12 Oct. 2021, [Peer-reviewed]
  English, Scientific journal
• Enhanced Self-Assembly and Mechanical Properties of Cellulose-Based Triblock Copolymers: Comparisons with Amylose-Based Triblock Copolymers
  Satoshi Katsuhara; Yasuko Takagi; Naoki Sunagawa; Kiyohiko Igarashi; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  ACS Sustainable Chemistry & Engineering, 9, 29, 9779, 9788, American Chemical Society (ACS), 08 Jul. 2021, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• One‐Shot Intrablock Cross‐Linking of Linear Diblock Copolymer to Realize Janus‐Shaped Single‐Chain Nanoparticles
  Kodai Watanabe; Noya Kaizawa; Brian J. Ree; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Angewandte Chemie International Edition, 60, 33, 18122, 18128, Wiley, 08 Jul. 2021, [Peer-reviewed]
  English, Scientific journal
• Doxorubicin Embedded into Nanofibrillated Bacterial Cellulose (NFBC) Produces a Promising Therapeutic Outcome for Peritoneally Metastatic Gastric Cancer in Mice Models via Intraperitoneal Direct Injection
  Hidenori Ando; Takashi Mochizuki; Amr S. Abu Lila; Shunsuke Akagi; Kenji Tajima; Kenji Fujita; Taro Shimizu; Yu Ishima; Tokuo Matsushima; Takatomo Kusano; Tatsuhiro Ishida
  Nanomaterials, 11, 7, 1697, 1697, MDPI AG, 28 Jun. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Natural materials such as bacterial cellulose are gaining interest for their use as drug-delivery vehicles. Herein, the utility of nanofibrillated bacterial cellulose (NFBC), which is produced by culturing a cellulose-producing bacterium (Gluconacetobacter intermedius NEDO-01) in a medium supplemented with carboxymethylcellulose (CMC) that is referred to as CM-NFBC, is described. Recently, we demonstrated that intraperitoneal administration of paclitaxel (PTX)-containing CM-NFBC efficiently suppressed tumor growth in a peritoneally disseminated cancer xenograft model. In this study, to confirm the applicability of NFBC in cancer therapy, a chemotherapeutic agent, doxorubicin (DXR), embedded into CM-NFBC, was examined for its efficiency to treat a peritoneally disseminated gastric cancer via intraperitoneal administration. DXR was efficiently embedded into CM-NFBC (DXR/CM-NFBC). In an in vitro release experiment, 79.5% of DXR was released linearly into the peritoneal wash fluid over a period of 24 h. In the peritoneally disseminated gastric cancer xenograft model, intraperitoneal administration of DXR/CM-NFBC induced superior tumor growth inhibition (TGI = 85.5%) by day 35 post-tumor inoculation, compared to free DXR (TGI = 62.4%). In addition, compared with free DXR, the severe side effects that cause body weight loss were lessened via treatment with DXR/CM-NFBC. These results support the feasibility of CM-NFBC as a drug-delivery vehicle for various anticancer agents. This approach may lead to improved therapeutic outcomes for the treatment of intraperitoneally disseminated cancers.
• Cyclization of PEG and Pluronic Surfactants and the Effects of the Topology on Their Interfacial Activity
  Tomohisa Watanabe; Satoru Chimura; Yubo Wang; Tomoko Ono; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Daichi Ida; Takuya Yamamoto
  Langmuir, 37, 23, 6974, 6984, American Chemical Society (ACS), 28 May 2021, [Peer-reviewed]
  English, Scientific journal
• Therapeutic efficacy of a paclitaxel-loaded nanofibrillated bacterial cellulose (PTX/NFBC) formulation in a peritoneally disseminated gastric cancer xenograft model
  Shunsuke Akagi; Hidenori Ando; Kenji Fujita; Taro Shimizu; Yu Ishima; Kenji Tajima; Tokuo Matsushima; Takatomo Kusano; Tatsuhiro Ishida
  International Journal of Biological Macromolecules, 174, 494, 501, Elsevier BV, Mar. 2021, [Peer-reviewed]
  English, Scientific journal
• Stretchable OFET Memories: Tuning the Morphology and the Charge-Trapping Ability of Conjugated Block Copolymers through Soft Segment Branching.
  Li-Che Hsu; Takuya Isono; Yan-Cheng Lin; Saburo Kobayashi; Yun-Chi Chiang; Dai-Hua Jiang; Chih-Chien Hung; Ender Ercan; Wei-Chen Yang; Hui-Ching Hsieh; Kenji Tajima; Toshifumi Satoh; Wen-Chang Chen
  ACS applied materials & interfaces, 13, 2, 2932, 2943, 20 Jan. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The mechanical properties and structural design flexibility of charge-trapping polymer electrets have led to their widespread use in organic field-effect transistor (OFET) memories. For example, in the electrets of polyfluorene-based conjugated/insulating block copolymers (BCPs), the confined fiberlike polyfluorene nanostructures in the insulating polymer matrix act as effective hole-trapping sites, leading to controllable memory performance through the design of BCPs. However, few studies have reported intrinsically stretchable charge-trapping materials and their memory device applications, and a practical method to correlate the thin-film morphology of BCP electrets with their charge-trapping ability has not yet been developed. In this study, a series of new conjugated/insulating BCPs, poly(9,9-di-n-hexyl-2,7-fluorene)-block-poly(δ-decanolactone)s (PF-b-PDL
  x
  , x = 1-3), as stretchable hole-trapping materials are reported. The linear and branched PDL blocks with comparable molecular weights were used to investigate the effect of polymer architecture on morphology and device performance. Moreover, the coverage area of the polyfluorene nanofibers on the BCP films was extracted from atomic force microscopy images, which can be correlated with the trapping density of the polymer electrets. The branched PDL segments not only improve stretchability but also tailor crystallinity and phase separation of the BCPs, thus increasing their charge-trapping ability. The OFET memory device with PF-b-PDL3 as the electret layer exhibited the largest memory window (102 V) and could retain its performance at up to 100% strain. This research highlights the importance of the BCP design for developing stretchable charge-trapping materials.
• Molecular Dynamics Simulation of Cellulose Synthase Subunit D Octamer with Cellulose Chains from Acetic Acid Bacteria: Insight into Dynamic Behaviors and Thermodynamics on Substrate Recognition
  Takuya Uto; Yuki Ikeda; Naoki Sunagawa; Kenji Tajima; Min Yao; Toshifumi Yui
  Journal of Chemical Theory and Computation, 17, 1, 488, 496, American Chemical Society (ACS), 31 Dec. 2020, [Peer-reviewed]
  English, Scientific journal
• Enhanced dispersion stability of gold nanoparticles by the physisorption of cyclic poly(ethylene glycol)
  Yubo Wang; Jose Enrico Q. Quinsaat; Tomoko Ono; Masatoshi Maeki; Manabu Tokeshi; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Shin-ichiro Sato; Yutaka Miura; Takuya Yamamoto
  Nature Communications, 11, 1, Springer Science and Business Media LLC, 30 Nov. 2020, [Peer-reviewed]
  English, Scientific journal, Abstract
  
  Nano-sized metal particles are attracting much interest in industrial and biomedical applications due to the recent progress and development of nanotechnology, and the surface-modifications by appropriate polymers are key techniques to stably express their characteristics. Herein, we applied cyclic poly(ethylene glycol) (c-PEG), having no chemical inhomogeneity, to provide a polymer topology-dependent stabilization for the surface-modification of gold nanoparticles (AuNPs) through physisorption. By simply mixing c-PEG, but not linear counterparts, enables AuNPs to maintain dispersibility through freezing, lyophilization, or heating. Surprisingly, c-PEG endowed AuNPs with even better dispersion stability than thiolated PEG (HS–PEG–OMe). The stronger affinity of c-PEG was confirmed by DLS, ζ-potential, and FT-IR. Furthermore, the c-PEG system exhibited prolonged blood circulation and enhanced tumor accumulation in mice. Our data suggests that c-PEG induces physisorption on AuNPs, supplying sufficient stability toward bio-medical applications, and would be an alternative approach to the gold–sulfur chemisorption.
• Nanofibrillated Bacterial Cellulose Modified with (3-Aminopropyl)trimethoxysilane under Aqueous Conditions: Applications to Poly(methyl methacrylate) Fiber-Reinforced Nanocomposites
  Kono, H.; Uno, T.; Tsujisaki, H.; Matsushima, T.; Tajima, K.
  ACS Omega, 5, 45, 29561, 29569, American Chemical Society ({ACS}), 17 Nov. 2020, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, 13483839
• Suzuki–Miyaura Catalyst-Transfer Polycondensation of Triolborate-Type Fluorene Monomer: Toward Rapid Access to Polyfluorene-Containing Block and Graft Copolymers from Various Macroinitiators
  Saburo Kobayashi; Kaiyu Fujiwara; Dai-Hua Jiang; Takuya Yamamoto; Kenji Tajima; Yasunori Yamamoto; Takuya Isono; Toshifumi Satoh
  Polymer Chemistry, 11, 42, 6832, 6839, Royal Society of Chemistry (RSC), 09 Oct. 2020, [Peer-reviewed]
  Scientific journal,

  In this study, we demonstrated that the Suzuki–Miyaura catalyst transfer polycondensation (SCTP) of the triolborate-type fluorene monomer, viz. potassium 2-(7-bromo-9,9-dihexyl-9H-fluorene-2-yl)triolborate, can be an efficient and versatile approach to the precise...

• Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures
  Takuya Isono; Ryoya Komaki; Chaehun Lee; Nao Kawakami; Brian J. Ree; Kodai Watanabe; Kohei Yoshida; Hiroaki Mamiya; Takuya Yamamoto; Redouane Borsali; Kenji Tajima; Toshifumi Satoh
  Communications Chemistry, 3, 1, Springer Science and Business Media LLC, 09 Oct. 2020, [Peer-reviewed]
  Scientific journal
• An organocatalytic ring-opening polymerization approach to highly alternating copolymers of lactic acid and glycolic acid
  Kaoru Takojima; Hiroshi Makino; Tatsuya Saito; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Polymer Chemistry, 11, 39, 6365, 6373, Royal Society of Chemistry (RSC), 07 Sep. 2020, [Peer-reviewed]
  English, Scientific journal,

  Highly regioselective ring-opening polymerization of optically active methylglycolides was achived using P₂-t-Bu/alcohol system to produce alternating copolymer of lactic acid and glycolic acid.

• Nanofibrillated Bacterial Cellulose Surface Modified with Methyltrimethoxysilane for Fiber-Reinforced Composites
  Kono, H.; Uno, T.; Tsujisaki, H.; Anai, H.; Kishimoto, R.; Matsushima, T.; Tajima, K.
  ACS Applied Nano Materials, 3, 8, 8232, 8241, American Chemical Society ({ACS}), 14 Aug. 2020, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Highly asymmetric lamellar nanostructures from nanoparticle-linear hybrid block copolymers.
  Kodai Watanabe; Satoshi Katsuhara; Hiroaki Mamiya; Yukihiko Kawamura; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Nanoscale, 12, 31, 16526, 16534, Royal Society of Chemistry (RSC), 13 Aug. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The highly asymmetric lamellar (A-LAM) nanostructure is one of the most important template geometries for block copolymer (BCP) lithography. However, A-LAM is unattainable from conventional BCPs, and there is no general molecular design strategy for A-LAM-forming BCP. Herein, a nanoparticle-linear hybrid BCP system is reported, which is designed based on the intramolecular crosslinking technique, as a remarkably effective platform to obtain the A-LAM morphology. The hybrid BCPs consisting of polystyrene single-chain nanoparticles and linear polylactide segments show a remarkable capability to form the A-LAM morphology in bulk, where a maximum width ratio of 4.1 between the two domains is obtained. This unusual phase behavior is attributed to the bulky and rigid characteristics of the nanoparticle block. Furthermore, the thin films of these hybrid BCPs show perpendicularly oriented A-LAM morphology on a chemically modified Si substrate, allowing promising application in the fabrication of asymmetric line-and-space nanopatterns.
• Programmed folding into spiro-multicyclic polymer topologies from linear and star-shaped chains
  Yoshinobu Mato; Kohei Honda; Brian J. Ree; Kenji Tajima; Takuya Yamamoto; Tetsuo Deguchi; Takuya Isono; Toshifumi Satoh
  Communications Chemistry, 3, 1, Springer Science and Business Media LLC, 04 Aug. 2020, [Peer-reviewed]
  Scientific journal
• Sweet Pluronic poly(propylene oxide)-b-oligosaccharide block copolymer systems: Toward sub-4 nm thin-film nanopattern resolution
  Mumtaz, M.; Takagi, Y.; Mamiya, H.; Tajima, K.; Bouilhac, C.; Isono, T.; Satoh, T.; Borsali, R.
  European Polymer Journal, 134, 109831, 109831, Elsevier BV, Jul. 2020, [Peer-reviewed]
  English, Scientific journal
• Carbohydrates as Hard Segments for Sustainable Elastomers: Carbohydrates Direct the Self-Assembly and Mechanical Properties of Fully Bio-Based Block Copolymers
  Takuya Isono; Saki Nakahira; Hui-Ching Hsieh; Satoshi Katsuhara; Hiroaki Mamiya; Takuya Yamamoto; Wen-Chang Chen; Redouane Borsali; Kenji Tajima; Toshifumi Satoh
  Macromolecules, 53, 13, 5408, 5417, American Chemical Society (ACS), 16 Jun. 2020, [Peer-reviewed]
  English, Scientific journal
• Detailed Structural Analyses of Nanofibrillated Bacterial Cellulose and Its Application as Binder Material for a Display Device.
  Kenji Tajima; Koutaro Tahara; Junya Ohba; Ryo Kusumoto; Ryota Kose; Hiroyuki Kono; Tokuo Matsushima; Koji Fushimi; Takuya Isono; Takuya Yamamoto; Toshifumi Satoh
  Biomacromolecules, 21, 2, 581, 588, 10 Feb. 2020, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, Nanofibrillated bacterial cellulose (NFBC) is produced by culturing a cellulose-producing bacterium under agitated aerobic conditions in a carboxymethylcellulose (CMC)-supplemented medium. Detailed structural analyses revealed that NFBC fiber widths varied with the degree of substitution of the CMC used, and zeta potential values decreased with the increment of CMC concentration in the medium. Transmission electron microscopy observation after immunostaining demonstrated that CMC molecules were present on the NFBC microfibril surfaces. We tested NFBC for utility as a binder for a display device that uses electrochromic (EC) material. Introduction of a quaternary ammonium group into the EC molecules enhanced their interactions with the negatively charged NFBC microfibrils. A casting process homogeneously adsorbed the EC molecules onto the surface of a transparent electrode with NFBC. A homogeneous color change was successfully observed upon applying an electric field, suggesting that NFBC could be used as a binder material for uniform surface adsorption.
• Facile synthesis of poly(trimethylene carbonate) by alkali metal carboxylate-catalyzed ring-opening polymerization
  Kaoru Takojima; Tatsuya Saito; Cedric Vevert; Viko Ladelta; Panayiotis Bilalis; Jun Watanabe; Shintaro Hatanaka; Takashi Konno; Takuya Yamamoto; Kenji Tajima; Nikos Hadjichristidis; Takuya Isono; Toshifumi Satoh
  Polymer Journal, 52, 1, 103, 110, Springer Science and Business Media LLC, Jan. 2020, [Peer-reviewed]
  Scientific journal
• Metallopolymer-block-oligosaccharide for sub-10 nm microphase separation
  Satoshi Katsuhara; Hiroaki Mamiya; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Polymer Chemistry, 11, 17, 2995, 3002, Royal Society of Chemistry (RSC), 2020, [Peer-reviewed]
  English, Scientific journal,

  The novel high-χ BCPs comprising poly(vinyl ferrocene) and oligosaccharides formed hexagonal cylinder morphology with d values of ∼8 nm. Lamellar morphology with d values of ∼9 nm was also realized by mixing these polymers and glucose.

• Structural and rheological characterization of bacterial cellulose gels obtained from Gluconacetobacter genus
  Numata, Y.; Kono, H.; Mori, A.; Kishimoto, R.; Tajima, K.
  Food Hydrocolloids, 92, 233, 239, Jul. 2019, [Peer-reviewed], [Last author]
  English, Scientific journal
• Installing a functional group into the inactive ω-chain end of PMMA and PS-: B -PMMA by terminal-selective transesterification
  Yoshida, K.; Yamamoto, T.; Tajima, K.; Isono, T.; Satoh, T.
  Polymer Chemistry, 10, 24, 3390, 3398, Jun. 2019, [Peer-reviewed]
  English, Scientific journal
• Trimethyl Glycine as an Environmentally Benign and Biocompatible Organocatalyst for Ring-Opening Polymerization of Cyclic Carbonate
  Tatsuya Saito; Kaoru Takojima; Takafumi Oyama; Shintaro Hatanaka; Takashi Konno; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  ACS Sustainable Chemistry & Engineering, 7, 9, 8868, 8875, 06 May 2019, [Peer-reviewed]
  English, Scientific journal
• Biodegradable Compatibilizers for Poly(hydroxyalkanoate)/Poly(ε-caprolactone) Blends through Click Reactions with End-Functionalized Microbial Poly(hydroxyalkanoate)s
  Takafumi Oyama; Shingo Kobayashi; Tetsuo Okura; Shunsuke Sato; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  ACS Sustainable Chemistry & Engineering, 7, 8, 7969, 7978, 15 Apr. 2019, [Peer-reviewed]
  English, Scientific journal
• Downsizing feature of microphase-separated structures via intramolecular crosslinking of block copolymers.
  Kodai Watanabe; Satoshi Katsuhara; Hiroaki Mamiya; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Chemical science, 10, 11, 3330, 3339, 21 Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Microphase separation of carbohydrate-based star-block copolymers with sub-10 nm periodicity
  Isono, T.; Kawakami, N.; Watanabe, K.; Yoshida, K.; Otsuka, I.; Mamiya, H.; Ito, H.; Yamamoto, T.; Tajima, K.; Borsali, R.; Satoh, T.
  Polymer Chemistry, 10, 9, 1119, 1129, Mar. 2019, [Peer-reviewed]
  English, Scientific journal
• A versatile synthetic strategy for macromolecular cages: intramolecular consecutive cyclization of star-shaped polymers.
  Yoshinobu Mato; Kohei Honda; Kenji Tajima; Takuya Yamamoto; Takuya Isono; Toshifumi Satoh
  Chemical science, 10, 2, 440, 446, 14 Jan. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Chain-End Functionalization with a Saccharide for 10 nm Microphase Separation: "classical" PS- b-PMMA versus PS- b-PMMA-Saccharide
  Yoshida, K.; Tanaka, S.; Yamamoto, T.; Tajima, K.; Borsali, R.; Isono, T.; Satoh, T.
  Macromolecules, 51, 21, 8870, 8877, 13 Nov. 2018, [Peer-reviewed]
  English, Scientific journal
• Synthesis, Isolation, and Properties of All Head-to-Tail Cyclic Poly(3-hexylthiophene): Fully Delocalized Exciton over the Defect-Free Ring Polymer
  Takuya Yamamoto; Masaaki Hosokawa; Minato Nakamura; Shin-ichiro Sato; Takuya Isono; Kenji Tajima; Toshifumi Satoh; Masamichi Sato; Yasuyuki Tezuka; Akinori Saeki; Yoshihiro Kikkawa
  Macromolecules, 51, 22, 9284, 9293, Nov. 2018, [Peer-reviewed]
  English, Scientific journal
• Facile and Efficient Modification of Polystyrene- block-poly(methyl methacrylate) for Achieving Sub-10 nm Feature Size
  Yoshida, K.; Tian, L.; Miyagi, K.; Yamazaki, A.; Mamiya, H.; Yamamoto, T.; Tajima, K.; Isono, T.; Satoh, T.
  Macromolecules, 51, 20, 8064, 8072, 23 Oct. 2018, [Peer-reviewed]
  English, Scientific journal
• Synthesis of μ-ABC Tricyclic Miktoarm Star Polymer via Intramolecular Click Cyclization.
  Tomoki Shingu; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Polymers, 10, 8, 877, 06 Aug. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Multicyclic Polymer Synthesis through Controlled/Living Cyclopolymerization of α,ω-Dinorbornenyl-Functionalized Macromonomers
  Takuya Isono; Tetsuya Sasamori; Kohei Honda; Yoshinobu Mato; Takuya Yamamoto; Kenji Tajima; Toshifumi Satoh
  Macromolecules, 51, 10, 3855, 3864, 22 May 2018, [Peer-reviewed]
  English, Scientific journal
• Alkali Metal Carboxylate as an Efficient and Simple Catalyst for Ring-Opening Polymerization of Cyclic Esters
  Tatsuya Saito; Yusuke Aizawa; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  Macromolecules, 51, 3, 689, 696, 13 Feb. 2018, [Peer-reviewed]
  English, Scientific journal
• Highly Ordered Cylinder Morphologies with 10 nm Scale Periodicity in Biomass-Based Block Copolymers
  Takuya Isono; Brian J. Ree; Kenji Tajima; Redouane Borsali; Toshifumi Satoh
  Macromolecules, 51, 2, 428, 437, 23 Jan. 2018, [Peer-reviewed]
  English, Scientific journal
• Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C.
  Shingo Nojima; Ayumi Fujishima; Koji Kato; Kayoko Ohuchi; Nobutaka Shimizu; Kento Yonezawa; Kenji Tajima; Min Yao
  Scientific reports, 7, 1, 13018, 13018, 12 Oct. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• One-Step Production of Amphiphilic Nanofibrillated Cellulose Using a Cellulose-Producing Bacterium.
  Kenji Tajima; Ryo Kusumoto; Ryota Kose; Hiroyuki Kono; Tokuo Matsushima; Takuya Isono; Takuya Yamamoto; Toshifumi Satoh
  Biomacromolecules, 18, 10, 3432, 3438, 09 Oct. 2017, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal
• Structural and mechanical characterization of bacterial cellulose-polyethylene glycol diacrylate composite gels
  Yukari Numata; Hiroyuki Kono; Minato Tsuji; Kenji Tajima
  CARBOHYDRATE POLYMERS, 173, 67, 76, Oct. 2017, [Peer-reviewed], [Last author]
  English, Scientific journal
• A facile strategy for manipulating micellar size and morphology through intramolecular cross-linking of amphiphilic block copolymers
  Ryoto Tanaka; Kodai Watanabe; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  POLYMER CHEMISTRY, 8, 23, 3647, 3656, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• NMR characterization of methylcellulose: Chemical shift assignment and mole fraction of monomers in the polymer chains
  Hiroyuki Kono; Sayaka Fujita; Kenji Tajima
  CARBOHYDRATE POLYMERS, 157, 728, 738, Feb. 2017, [Peer-reviewed], [Last author]
  English, Scientific journal
• Synthesis of Well-Defined Three- and Four-Armed Cage-Shaped Polymers via "Topological Conversion" from Trefoil- and Quatrefoil-Shaped Polymers
  Yusuke Satoh; Hirohiko Matsuno; Takuya Yamamoto; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  MACROMOLECULES, 50, 1, 97, 106, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Heterologous expression of cellulose synthases and trial of synthesis of cellulose in vitro to elucidate the mechanism of cellulose synthesis in nature
  Naoki Sunagawa; Kenji Tajima; Masahiro Samejima; Kiyohiko Igarashi
  Kobunshi, 66, 6, 288, 289, 2017, [Peer-reviewed]
  Japanese, Scientific journal
• In vitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria
  Kenji Tajima; Xuerong Han; Yoshiki Hashimoto; Yasuharu Satoh; Toshifumi Satoh; Seiichi Taguchi
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122, 6, 660, 665, Dec. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• NMR characterization of sodium carboxymethyl cellulose 2: Chemical shift assignment and conformation analysis of substituent groups
  Hiroyuki Kono; Kazuhiro Oshima; Hisaho Hashimoto; Yuuichi Shimizu; Kenji Tajima
  CARBOHYDRATE POLYMERS, 150, 241, 249, Oct. 2016, [Peer-reviewed], [Last author]
  English, Scientific journal
• NMR characterization of sodium carboxymethyl cellulose: Substituent distribution and mole fraction of monomers in the polymer chains
  Hiroyuki Kono; Kazuhiro Oshima; Hisaho Hashimoto; Yuuichi Shimizu; Kenji Tajima
  CARBOHYDRATE POLYMERS, 146, 1, 9, Aug. 2016, [Peer-reviewed], [Last author]
  English, Scientific journal
• Self-Assembly of Maltoheptaose-block-polycaprolactone Copolymers: Carbohydrate-Decorated Nanoparticles with Tunable Morphology and Size in Aqueous Media
  Takuya Isono; Kana Miyachi; Yusuke Satoh; Ryosuke Nakamura; Yao Zhang; Issei Otsuka; Kenji Tajima; Toyoji Kakuchi; Redouane Borsali; Toshifumi Satoh
  MACROMOLECULES, 49, 11, 4178, 4194, Jun. 2016, [Peer-reviewed]
  English, Scientific journal
• Advanced functionalization of polyhydroxyalkanoate via the UV-initiated thiol-ene click reaction
  Kenji Tajima; Kosuke Iwamoto; Yasuharu Satoh; Ryosuke Sakai; Toshifumi Satoh; Tohru Dairi
  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100, 10, 4375, 4383, May 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Mechanical properties of a bacterial cellulose/polyethylene glycol gel with a peripheral region crosslinked by polyethylene glycol diacrylate
  Yukari Numata; Shiro Masaki; Kenji Tajima
  POLYMER JOURNAL, 48, 3, 317, 321, Mar. 2016, [Peer-reviewed], [Last author]
  English, Scientific journal
• Synthesis of Well-Defined Amphiphilic Star-Block and Miktoarm Star Copolyethers via t-Bu-P-4-Catalyzed Ring-Opening Polymerization of Glycidyl Ethers
  Yusuke Satoh; Kana Miyachi; Hirohiko Matsuno; Takuya Isono; Kenji Tajima; Toyoji Kakuchi; Toshifumi Satoh
  MACROMOLECULES, 49, 2, 499, 509, Jan. 2016, [Peer-reviewed]
  English, Scientific journal
• Intramolecular olefin metathesis as a robust tool to synthesize single-chain nanoparticles in a size-controlled manner
  Kodai Watanabe; Ryoto Tanaka; Kenji Takada; Myung-Jin Kim; Jae-Suk Lee; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  POLYMER CHEMISTRY, 7, 29, 4782, 4792, 2016, [Peer-reviewed]
  English, Scientific journal
• In Vivo Curdlan/Cellulose Bionanocomposite Synthesis by Genetically Modified Gluconacetobacter xylinus
  Ju Fang; Shin Kawano; Kenji Tajima; Tetsuo Kondo
  BIOMACROMOLECULES, 16, 10, 3154, 3160, Oct. 2015, [Peer-reviewed]
  English, Scientific journal
• Controlled/Living Ring-Opening Polymerization of Glycidylamine Derivatives Using t-Bu-P-4/Alcohol Initiating System Leading to Polyethers with Pendant Primary, Secondary, and Tertiary Amino Groups
  Takuya Isono; Shunsuke Asai; Yusuke Satoh; Toshimitsu Takaoka; Kenji Tajima; Toyoji Kakuchi; Toshifumi Satoh
  MACROMOLECULES, 48, 10, 3217, 3229, May 2015, [Peer-reviewed]
  English, Scientific journal
• Bacterial cellulose gels with high mechanical strength
  Yukari Numata; Tadanori Sakata; Hidemitsu Furukawa; Kenji Tajima
  MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 47, 57, 62, Feb. 2015, [Peer-reviewed], [Last author]
  English, Scientific journal
• Organophosphate-catalyzed bulk ring-opening polymerization as an environmentally benign route leading to block copolyesters, end-functionalized polyesters, and polyester-based polyurethane
  Tatsuya Saito; Yusuke Aizawa; Kenji Tajima; Takuya Isono; Toshifumi Satoh
  POLYMER CHEMISTRY, 6, 24, 4374, 4384, 2015, [Peer-reviewed]
  English, Scientific journal
• Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene
  Xuerong Han; Yasuharu Satoh; Yumi Kuriki; Teruyuki Seino; Shinji Fujita; Takanori Suda; Takanori Kobayashi; Kenji Tajima
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 118, 5, 514, 519, Nov. 2014, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Stereoblock-like Brush Copolymers Consisting of Poly(L-lactide) and Poly(D-lactide) Side Chains along Poly(norbornene) Backbone: Synthesis, Stereocomplex Formation, and Structure-Property Relationship
  Takuya Isono; Yohei Kondo; Shun Ozawa; Yougen Chen; Ryosuke Sakai; Shin-ichiro Sato; Kenji Tajima; Toyoji Kakuchi; Toshifumi Satoh
  MACROMOLECULES, 47, 20, 7118, 7128, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• Characterization of an Alginate Lyase, FlAlyA, from Flavobacterium sp Strain UMI-01 and Its Expression in Escherichia coli
  Akira Inoue; Kohei Takadono; Ryuji Nishiyama; Kenji Tajima; Takanori Kobayashi; Takao Ojima
  MARINE DRUGS, 12, 8, 4693, 4712, Aug. 2014, [Peer-reviewed]
  English, Scientific journal
• Synthesis of Linear, Cyclic, Figure-Eight-Shaped, and Tadpole-Shaped Amphiphilic Block Copolyethers via t-Bu-P-4-Catalyzed Ring-Opening Polymerization of Hydrophilic and Hydrophobic Glycidyl Ethers
  Takuya Isono; Yusuke Satoh; Kana Miyachi; Yougen Chen; Shin-ichiro Sato; Kenji Tajima; Toshifumi Satoh; Toyoji Kakuchi
  MACROMOLECULES, 47, 9, 2853, 2863, May 2014, [Peer-reviewed]
  English, Scientific journal
• One-step production of nanofibrillated bacterial cellulose (NFBC) from waste glycerol using Gluconacetobacter intermedius NEDO-01
  Ryota Kose; Naoki Sunagawa; Makoto Yoshida; Kenji Tajima
  CELLULOSE, 20, 6, 2971, 2979, Dec. 2013, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Cellulose complementing factor (Ccp) is a new member of the cellulose synthase complex (terminal complex) in Acetobacter xylinum
  Naoki Sunagawa; Takaaki Fujiwara; Takanori Yoda; Shin Kawano; Yasuharu Satoh; Min Yao; Kenji Tajima; Tohru Dairi
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115, 6, 607, 612, Jun. 2013, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• The c-di-GMP recognition mechanism of the PilZ domain of bacterial cellulose synthase subunit A
  Takaaki Fujiwara; Keisuke Komoda; Naofumi Sakurai; Kenji Tajima; Isao Tanaka; Min Yao
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 431, 4, 802, 807, Feb. 2013, [Peer-reviewed]
  English, Scientific journal
• Cellulose production by Enterobacter sp CJF-002 and identification of genes for cellulose biosynthesis
  Naoki Sunagawa; Kenji Tajima; Mariko Hosoda; Shin Kawano; Ryota Kose; Yasuharu Satoh; Min Yao; Tohru Dairi
  CELLULOSE, 19, 6, 1989, 2001, Dec. 2012, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol
  Yasuharu Satoh; Kenji Tajima; Masanobu Munekata; Jay D. Keasling; Taek Soon Lee
  METABOLIC ENGINEERING, 14, 6, 603, 610, Nov. 2012, [Peer-reviewed]
  English, Scientific journal
• In vitro synthesis of polyhydroxyalkanoate (PHA) incorporating lactate (LA) with a block sequence by using a newly engineered thermostable PHA synthase from Pseudomonas sp SG4502 with acquired LA-polymerizing activity
  Kenji Tajima; Xuerong Han; Yasuharu Satoh; Ayako Ishii; Yuji Araki; Masanobu Munekata; Seiichi Taguchi
  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94, 2, 365, 376, Apr. 2012, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Engineering of a Tyrosol-Producing Pathway, Utilizing Simple Sugar and the Central Metabolic Tyrosine, in Escherichia coli
  Yasuharu Satoh; Kenji Tajima; Masanobu Munekata; Jay D. Keasling; Taek Soon Lee
  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60, 4, 979, 984, Feb. 2012, [Peer-reviewed]
  English, Scientific journal
• Chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA) incorporating 2-hydroxybutyrate by wild-type class I PHA synthase from Ralstonia eutropha
  Xuerong Han; Yasuharu Satoh; Toshifumi Satoh; Ken' ichiro Matsumoto; Toyoji Kakuchi; Seiichi Taguchi; Tohru Dairi; Masanobu Munekata; Kenji Tajima
  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92, 3, 509, 517, Nov. 2011, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate
  Y. Satoh; K. Tajima; S. Nakamoto; H. Xuerong; T. Matsushima; T. Ohshima; S. Kawano; T. Erata; T. Dairi; M. Munekata
  JOURNAL OF APPLIED MICROBIOLOGY, 111, 4, 811, 817, Oct. 2011, [Peer-reviewed]
  English, Scientific journal
• Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli
  Jumiarti Agus; Prihardi Kahar; Manami Hyakutake; Satoshi Tomizawa; Hideki Abe; Takeharu Tsuge; Yasuharu Satoh; Kenji Tajima
  POLYMER DEGRADATION AND STABILITY, 95, 12, 2250, 2254, Dec. 2010, [Peer-reviewed]
  English, Scientific journal
• Structure of bacterial cellulose synthase subunit D octamer with four inner passageways
  Song-Qing Hu; Yong-Gui Gao; Kenji Tajima; Naoki Sunagawa; Yong Zhou; Shin Kawano; Takaaki Fujiwara; Takanori Yoda; Daisuke Shimura; Yasuharu Satoh; Masanobu Munekata; Isao Tanaka; Min Yao
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107, 42, 17957, 17961, Oct. 2010, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Development of a New Conversion Process Consisting of Hydrothermal Treatment and Catalytic Reaction Using ZrO2-FeO (X) Catalyst to Convert Fermentation Residue into Useful Chemicals
  Satoshi Funai; Yumi Satoh; Yasuharu Satoh; Kenji Tajima; Teruoki Tago; Takao Masuda
  TOPICS IN CATALYSIS, 53, 7-10, 654, 658, Jun. 2010, [Peer-reviewed]
  English, Scientific journal
• Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS)
  Xuerong Han; Yasuharu Satoh; Kenji Tajima; Tokuo Matsushima; Masanobu Munekata
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, 6, 517, 523, Dec. 2009, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Chemo-Enzymatic Synthesis of Poly(lactate-co-(3-hydroxybutyrate)) by a Lactate-Polymerizing Enzyme
  Kenji Tajima; Yasuharu Satoh; Toshifumi Satoh; Rumi Itoh; Xuerong Han; Seiichi Taguchi; Toyoji Kakuchi; Masanobu Munekata
  MACROMOLECULES, 42, 6, 1985, 1989, Mar. 2009, [Peer-reviewed]
  English, Scientific journal
• 微生物を活用したバイオポリマーへの物質変換
  佐藤 康治; 田島 健次
  環境技術 38, 14, 18, 2009
  Scientific journal
• Kinetic Analysis of Engineered Polyhydroxyalkanoate Synthases with Broad Substrate Specificity
  Ken'ichiro Matsumoto; Fumi Shozui; Yasuharu Satoh; Kenji Tajima; Masanobu Munekata; Seiichi Taguchi
  POLYMER JOURNAL, 41, 3, 237, 240, 2009, [Peer-reviewed]
  English, Scientific journal
• Nonvolatile and Shape-Memorized Bacterial Cellulose Gels Swollen by Poly(ethylene glycol)
  Yukari Numata; Kazumi Muromoto; Hidemitsu Furukawa; Jian Ping Gong; Kenji Tajima; Masanobu Munekata
  POLYMER JOURNAL, 41, 7, 524, 525, 2009, [Peer-reviewed]
  English, Scientific journal
• A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme
  Seiichi Taguchi; Miwa Yamadaa; Ken'ichiro Matsumoto; Kenji Tajima; Yasuharu Satoh; Masanobu Munekata; Katsuhiro Ohno; Katsunori Kohda; Takashi Shimamura; Hiromi Kambe; Shusei Obata
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105, 45, 17323, 17327, Nov. 2008, [Peer-reviewed]
  English, Scientific journal
• Regulation of endoglucanase gene (cmcax) expression in Acetobacter xylinum
  Shin Kawano; Kenji Tajima; Hiroyuki Kono; Yukari Numata; Hitomi Yamashita; Yasuharu Satoh; Masanobu Munekata
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106, 1, 88, 94, Jul. 2008, [Peer-reviewed]
  English, Scientific journal
• Purification, Crystallization and Preliminary X-Ray Studies of AxCesD Required for Efficient Cellulose Biosynthesis in Acetobacter xylinum
  SongQing Hu; YongGui Gao; Kenji Tajima; Min Yao; Takanori Yoda; Daisuke Shimura; Yasuharu Satoh; Shin Kawano; Isao Tanaka; Masanobu Munekata
  PROTEIN AND PEPTIDE LETTERS, 15, 1, 115, 117, Jan. 2008, [Peer-reviewed]
  English, Scientific journal
• In vitro growth and differentiated activities of human periodontal ligament fibroblasts cultured on salmon collagen gel
  Nobuhiro Nagai; Kazuo Mori; Yasuharu Satoh; Noritaka Takahashi; Shunji Yunoki; Kenji Tajima; Masanobu Munekata
  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 82A, 2, 395, 402, Aug. 2007, [Peer-reviewed]
  English, Scientific journal
• Preparation of salmon atelocollagen fibrillar gel and its application to the cell culture
  Nobuhiro Nagai; Shunji Yunoki; Takeshi Suzuki; Yasuharu Satoh; Kenji Tajima; Masanobu Munekata
  CELL TECHNOLOGY FOR CELL PRODUCTS, 321, +, 2007, [Peer-reviewed]
  English, International conference proceedings
• Structural characterization of the Acetobacter xylinum endo-beta-1,4-glucanase CMCax required for cellulose biosynthesis
  Yoshiaki Yasutake; Shin Kawano; Kenji Tajima; Min Yao; Yasuharu Satoh; Masanobu Munekata; Isao Tanaka
  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 64, 4, 1069, 1077, Sep. 2006, [Peer-reviewed]
  English, Scientific journal
• Activities of MC3T3-E1 cells cultured on gamma-irradiated salmon atelocollagen scaffold
  Nobuhiro Nagai; Takafumi Anzawa; Yasuharu Satoh; Takeshi Suzuki; Kenji Tajima; Masanobu Munekata
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 101, 6, 511, 514, Jun. 2006, [Peer-reviewed]
  English, Scientific journal
• Enzymatic synthesis and direct observation of poly are chains of phosphate molecules and are often distributed in osteoblasts. The purpose of this study was to clarify the effects of Poly(P) on periodontal regeneration. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and its gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain reaction and von Kossa staining. Alkaline phosphatase activity was also determined. The potential role of Poly(P) in bone formation was assessed using a rat alveolar bone regeneration model. Poly(P) exhibited antibacterial activity against Prophyromonas gingivalis and Streptococcus mutans, induced expression of osteopontin and osteocalcine and increased alkaline phosphatase activity in MC 3T3-E1 cells. Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays roles of osteoblastic differentiation, activation and bone mineralization and possesses antibacterial activity. Thus, local delivery of poly(P) may have adjunctive therapeutic benefits in periodontal disease.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), 12558099
• 2段階バイオリアクターを用いた生分解性プラスチックの連続合成
  基盤的研究開発育成事業 研究開発シーズ育成補助金
  Jul. 2001 - Mar. 2002
  田島 健次
  ノーステック財団, Principal investigator, Competitive research funding
• セルロース合成に関与しているセルラーゼの立体構造解析とその機能解明
  北海道大学クラーク記念財団助成事業 独創的研究助成
  Apr. 2001 - Mar. 2002
  田島 健次
  北海道大学クラーク記念財団, Principal investigator, Competitive research funding
• 固定化菌体・酵素を用いた生分解性プラスチックの連続合成
  基盤的研究開発育成事業 研究開発シーズ育成補助金
  Jul. 1999 - Mar. 2000
  田島 健次
  ノーステック財団, Principal investigator, Competitive research funding
• 遺伝子組換えによる高機能性セルロース誘導体のin vivo合成
  研究助成
  Apr. 1999 - Mar. 2000
  田島 健次
  ゼネラル石油研究医奨励財団, Principal investigator, Competitive research funding
• 歯プレセメント質由来糖タンパク質の構造と機能の解明
  科学研究費補助金 萌芽研究
  Apr. 1997 - Mar. 1999
  棟方 正信
  日本学術振興会, Competitive research funding
• 固定化菌体を用いた生分解性プラスチックの連続合成
  産業化研究開発支援事業 研究開発産業化促進補助金
  Apr. 1997 - Mar. 1999
  田島 健次
  ノーステック財団, Principal investigator, Competitive research funding
• Development of High Performance Temporarily Artificial Skin made from Salmon Collagen and Squid Chitin
  Grants-in-Aid for Scientific Research
  1997 - 1999
  TAKAI Mitsuo; SHIBA Toshikazu; ERATA Tomoki; MUNEKATA Masanobu; TAJIMA Kenji; FUJIWARA Masashi
  The squid chitin sheet prepared from the squid chitin suspension by air-decompression was used as the base sheet for temporarily artificial skin. The squid chitin sheet was laminated with salmon collagen for the growth of human skin cells. The chitin sheet laminated with salmon collagen showed clearly the superior attachment of human skin cells to the chitin sheet only as well as the chitin sheet laminated with the calf collagen. The growth of human skin cells on the chitin sheet laminated with salmon collagen was activated 1.3 times than that on the sheet laminated with calf collagen and 1.5 times than that on the chitin sheet only. The growing cells on the chitin sheet laminated with salmon collagen were confirmed as the normal cells by the DNA labeling index method. The chitin sheet laminated with salmon collagen showed the high bio-compatibility on the several bio-safety tests with the mouse or the rabbit. The continuous paper machine was developed for the manufacturing of the temporarily artificial skin from the squid chitin and the salmon collagen.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, 09555294
• 歯プレセメント質由来糖タンパク質の構造と機能の解明
  科学研究費助成事業
  1997 - 1998
  棟方 正信; 田島 健次; 柴 肇一
  本年度は,昨年度報告したヒト歯肉線維芽細胞を走化させるウシ歯根表面プレセメント質由来66KDaタンパクを,HPLCゲル濾過,HPLC陰イオン交換,HPLCハイドロキシアパタイト,HPLC-逆相,SDS-PAGE等にて多量に単離精製し,その走化活性及び増殖活性について既知物質との相違を検討した。歯周組織に存在する歯周靭帯細胞及び歯肉線維芽細胞に対し細胞走化活性がすでに報告されているPDGF-BB,AB,AA等に比べ,66kDaタンパクは約1/100の濃度で歯肉線維芽細胞に対し細胞走化活性を示したが,細胞増殖活性はPDGF-BB,AB,AA等の約10倍濃度を必要とした。すなわち66kDaタンパクは細胞走化活性に特異性がある物質と考えられた。またPDGF-BBとの併用では走化活性の相加効果が認められ,すでに前臨床での効果が報告されているPDGF-BBとIGF-Iの組み合わせより高い走化活性を示し,臨床応用が期待できる結果を得た。
  γ線照射したウシ歯セメント質粒子をゼラチン多孔性膜に包埋し,歯肉剥離掻爬手術(in vivo:サル)に用いた場合,歯周靭帯形成がなされず歯槽骨再生のみが進行し,歯槽骨が歯根面に接着する,いわゆるアンキロージスが認められた。γ線照射したウシ歯セメント質粒子のPBS(-)抽出物のHPLCゲル濾過では,全体に抽出物の低分子化認められた。またSDS-PAGEでは,66kDaタンパクは認められなかったことから,66kDaタンパクは歯周靭帯形成(分化)にも関与している可能性が示唆された。
  日本学術振興会, 萌芽的研究, 北海道大学, 09875238
• Biosynthesis and Properties Control of Bacterial Cellulose
  Grants-in-Aid for Scientific Research
  1996 - 1997
  TAKAI Mitsuo; TAJIMA Kenji; FUJIWARA Masashi; SHIBA Toshikazu; ERATA Tomoki; MUNEKATA Masanobu
  Bacterial cellulose (BC) productivity has been enhanced by the presence of 3.5% concentration of yeast extract in the medium as high as twice of the standard culture(0.5% yeast extract). Cell growth increased markedly with an increase in yeast extract concentration. pH of the medium decreased from the initial 5.9 to the range of 3.5-4.5 during 2 days incubation, however, after the prolonged incubation gradual increasing in pH was observed. Glucose as a carbon source was nevertheless consumed thoroughly during 4 days incubation, cellulose production proceeded slowly as long as 15 days incubation. An excess of yeast extract in the medium appear to play an role in the activator for cellulose biosynthesis. Bacterial cellulose (NBC) produced by Acetobacter xylinum (A.xylinum) has both great mechanical strength and biodegradability.The properties were controlled by the incorporation of water-soluble polymers (WSP) such as carboxymethyl cellulose (CMC), methyl cellulose (MC) and polyethylene glycol (PEG). Small amounts of the WSPs were added to the standerd Hestrin-Schramm medium.The WSPs were incorporated into BC during incubation. The cellulose composite (BCC) will be a kind of interpenetrating polymer.The BCC yield by weight were 1.5 times for MC and 1.8 times for CMC as compared with the weight of NBC control. The contents of MC and CMC in the BCC were checked by elemental analysis of C%, 45% for CMC and 20% for MC,respectively. From the X-ray analysis, the diffraction of (110) appeared sharply as a resuli of the disorientation of (110). The WSPs influence the preferential orientation of BC.Young's modulus of the BCC was about 1-3 times that of NBC (30-40GPa). BCC (CMC) was degraded completely by cellulase for 6 hr, while only 20-40% of BCC (MC) was degraded for 48hr. For degadation test in native soil, all BCC samples were degaded considerably at 30ﾟC for 4 weeks as well as NBC.There is some possibility to control a biodegadability of BC.The method of BCC production will be used to synthesize a new type of BC having both excellent biodegradability and other functions. The most effective incubation methods for NBC production were examined in various mode of bioreactor, and then on the basis of the results, the bioreactor with rotating-disk has been designed.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, 08455445
• Reactor Design for Biocellulose Production to the Aims of Industrial Process
  Grants-in-Aid for Scientific Research
  1995 - 1996
  TAKAI Mitsuo; TAJIMA Kenji; FUJIWARA Masashi; SHIBA Toshikazu; ERATA Tomoki; MUNEKATA Masanobu
  In this study, the most effective incubation methods for biocellulose production were examined in various mode of bioreactor, and then on the basis of the results, the bioreactor with rotating-disk has been designed. This is composed of horizontal pyrexcylinder of 20cm long and 15cm diameter, and stainless-made side plates equiped with one side of motor-drive, openings for culture medium and aeration, and with another side of pH and oxygen electrodes, two valves. Warmed water is passed through a thermojacked under the cylinder maintained at 28(]SY+-[)1ﾟC.pH in culture medium was automatically controlled by 1% NaOH aqueous solution. Aeration was done only into gas-phase by the filtered-air. Initial volume of the medium is 1800ml, that is half volume of the cylinder. Stainless mesh-disk from 6 to 16mesh of 14.4cm diameter were used as a supporter for bacterial cells. 60ml of preculture was inoculated into the reactor horizontally rotating 2-5rpm at 28ﾟC,and then incubated for 6days with aeration of 2000ml/min. The incubation was repeated 9times. Then total yealds of biocellulose has been examined for rotating speed and number of the disk, or pH control and aeration conditions. The most effective incubation conditions for the reactor of biocellulose production were as follows. 14mesh stainless disk was the most effective supporter for bacterial cells. Although the bioreactor produced about the same amount of biocellulose with respect to rotating speed of the disk, 30 and 50rpm, in the case of 50rpm incubation, there was some scattering of biocellulose too fast to retain on the disk. So the optimum rotating speed is 30rpm. Aeration into gas-phase would contribute to produce more biocellulose and to enhance physical properties of the biocellulose, especially, to increase in Young's modulus of the sheets.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), HOKKAIDO UNIVERSITY, 07555674

• ナノセルロース組成物、繊維及びシート、並びに繊維の製造方法
  Patent right, 田島 健次; 辻崎 晴人, 国立大学法人北海道大学
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• セルロースとヒアルロン酸との複合材、これを生産する細菌およびこれを製造する方法
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• セルロースとヒアルロン酸との複合材、これを生産する細菌およびこれを製造する方法
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  202103008118279818
• Bacterial cellulose and bacterium producing it
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• セルロースとヒアルロン酸との複合材、これを生産する細菌およびこれを製造する方法
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  202103008118279818
• BACTERIAL CELLULOSE AND BACTERIUM PRODUCING SAME
  Patent right
  Korena patent 2077716
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• バクテリアセルロースと樹脂との複合糸およびその製造方法
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  202003021102382014
• ブロック共重合体及びこれを含む樹脂組成物、並びにブロック共重合体の製造方法
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  WO2018-038055, 01 Mar. 2018
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• Bacterial cellulose and bacteriu, producing same
  Patent right, Kenji Tajima; Ryota Kose
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• Bacterial cellulose and bacterium producing it
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• Bacterial cellulose and bacterium producing same
  Patent right, Kenji Tajima; Ryota Kose
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  Patent right, Kenji Tajima; Ryota Kose
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• 新規のアルギン酸資化菌、その細菌が産生するアルギン酸を分解する酵素を含む菌抽出液、それらを用いてオリゴ糖、不飽和単糖、ないしα-ケト酸を製造する方法
  Patent right, 尾島 孝男; 田島 健次; 小林 孝紀, 国立大学法人北海道大学, 公益財団法人函館地域産業振興財団
  特願2012-067226, 23 Mar. 2012
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  特許第6025018号
  21 Oct. 2016
  201603003667727782
• Bacterial cellulose and bacterium producing same
  Patent right, Kenji Tajima; Ryota Kose
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• バクテリアセルロースおよびこれを生産する細菌
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• 共重合樹脂組成物、成形品、及び共重合樹脂組成物の製造方法
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• ポリヒドロキシアルカノエートの製造方法
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  特許第5555975号
  13 Jun. 2014
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• 熱可塑性樹脂の成形方法及び成形品
  Patent right, 原田 忠克; 山中 康生; ▲柳▼沼 秀和; 棟方 正信; 田島 健次; 佐藤 康治; 松島 得雄, 株式会社リコー
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  特許第5556010号
  13 Jun. 2014
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  Patent right, ▲柳▼沼 秀和; 原田 忠克; 山中 康生; 棟方 正信; 田島 健次; 佐藤 康治; 松島 得雄, 株式会社リコー
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  特許第5556009号
  13 Jun. 2014
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• 樹脂組成物及びこれを用いた成形品
  Patent right, 山中 康生; 原田 忠克; ▲柳▼沼 秀和; 棟方 正信; 田島 健次; 佐藤 康治; 松島 得雄, 株式会社リコー
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  特許第5509588号
  04 Apr. 2014
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• 導電性材料用支持体
  Patent right, 田島 健次; 棟方 正信; 松島 得雄, 国立大学法人北海道大学
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  特許第5303338号
  28 Jun. 2013
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  Patent right, 平野 成伸; 近藤 均; 田島 健次; 棟方 正信; 松島 得雄, 株式会社リコー, 国立大学法人北海道大学
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  17 May 2013
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  17 May 2013
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  Patent right, 棟方 正信; 田島 健次; 土谷 穏史, サンメディカル株式会社
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  特開2010-017361, 28 Jan. 2010
  201003016887111120
• ポリヒドロキシアルカノエート生成能を有する新規微生物、ポリヒドロキシアルカノエート重合酵素、およびこれをコードする遺伝子
  Patent right, 佐藤 康治; 田島 健次; 棟方 正信; 松島 得雄, 株式会社アグリバイオインダストリ, 国立大学法人 北海道大学
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  特開2010-017124, 28 Jan. 2010
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