Researcher Profile and Settings

Alias Name

Gowda

Affiliation

• Faculty of Health Sciences　Health Sciences　Health Sciences

Job Title

• Associate Professor

Degree

• PhD in Life Sciences（2016/03 Hokkaido University）

Contact information

• siddabasavegowda.bommegowdahs.hokudai.ac.jp

URL

https://www.hs.hokudai.ac.jp/archives/staff/22040/
https://www.hs.hokudai.ac.jp/e/archives/staff/1934/

Research funding number

• 50800504

J-Global ID

• 201901008474025035

Profile

• I am a bioanalytical chemist with more than 10 years of experience utilizing liquid-chromatography -mass spectrometry (LC-MS) to study food and biological systems. I have worked on lipid chemistry focusing on its preparation, extraction, biochemistry, and analysis (lipidomics). My research involves the study of food-derived sphingolipids (SL), obesity associated with SL metabolism, and analysis technique development including lipid mediators such as sphingosine-1-phosphate.  I am also working on numerous applications of targeted and untargeted mass spectrometry to discover lipid biomarkers in cells, murine, and human samples of various pathological conditions such as obesity, myocardial infarction, and influenza infection.  Current interests include discovering novel functions and developing analytical strategies for lipids (FAHFA: fatty acid esters of hydroxy fatty acids, oxidized fatty acids) and gut-microbial metabolites (SCFA: short-chain fatty acids, short-chain fatty acid esters of hydroxy fatty acids (SFAHFA). I also work on characterizing unknown lipids and compositions in functional foods with the aid of LC-MS.

  
  

  I am looking forward to collaborating with clinicians, food chemists, and biologists interested in lipid analysis and functional studies. Feel free to contact me for any questions and discussions. ​

Research Interests

• Functional food and Obesity   Analytical chemistry   sphingolipids and FAHFAs   LC-MS   lipidomics   lipid chemistry   Food Science   

Research Areas

• Life sciences / Functional biochemistry
• Life sciences / Bioorganic chemistry / Lipid Chemistry
• Natural sciences / Basic analysis / Lipidomics and Functional lipids
• Nanotechnology/Materials / Chemical biology / Lipids synthesis, analysis and applied sciences

Educational Organization

•  Bachelor's degree program　Departments of Health Sciences, School of Medicine
•  Master's degree program　Graduate School of Global Food Resources
•  Doctoral (PhD) degree program　Graduate School of Global Food Resources

Academic & Professional Experience

• 2023/04 - Today Hokkaido University Faculty of Health Sciences /Graduate School of Global Food Resources Associate Professor
• 2020/10 - 2023/04 Hokkaido University Faculty of Health Sciences, Graduate School of Global Food Resources Lecturer
• 2022/11 - 2022/12 University of South Florida
• 2019/06 - 2020/09 Hokkaido University Faculty of Health Sciences Assistant professor
• 2017/03 - 2019/04 RIKEN Center for Integrative Medical Sciences, Japan Postdoctoral Fellow
• 2016/04 - 2017/02 Hokkaido University, Japan Research Fellow
• 2015/11 - 2016/03 Hokkaido University, Japan Research Assitant
• 2011/10 - 2012/09 Yukthi Learning Center, India Assistant professor
• 2011/06 - 2011/09 Akaash IIT-JEE, India Assistant professor
• 2010/08 - 2011/05 Nimbus Tutorials, India Lecture in Chemistry
• 2009/08 - 2010/07 University of Mysore, India Lecture in Chemistry

Education

• 2012/10 - 2016/03  Hokkaido University  Life Science  PhD
• 2007/06 - 2009/07  University of Mysore  Chemsitry  Master degree

Association Memberships

• Triglyceride deposit cardiomyovasculopathy society (TGCV), Japan   Japanese Society of Clinical Chemistry   American Society for Mass Spectrometry   Japanese Society for Biomedical Mass Spectrometry   American Chemical Society   International Lipidomics Society   THE SOCIETY OF POLYMER SCIENCE, JAPAN   

Research Activities

Published Papers

• Dissecting new lipids and their composition in herbal tea using untargeted LC/MS

  Lipsa Rani Nath, Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Shu Ping Hui

  Food Chemistry 447 138941 - 138941 0308-8146 2024/07
• Regio-specific lipid fingerprinting of edible sea cucumbers using LC/MS.

  Yusuke Minami, Siddabasave Gowda B Gowda, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui

  Food research international (Ottawa, Ont.) 184 114253 - 114253 2024/05 

  Sea cucumbers are a rich source of bioactive compounds and are gaining popularity as nutrient-rich seafood. They are consumed as a whole organism in Pacific regions. However, limited data are available on the comparison of their lipid composition and nutritional value. In this study, untargeted liquid chromatography/mass spectrometry was applied to comprehensively profile lipids in the skin, meat, and intestinal contents of three color-distinct edible sea cucumbers. Multivariate principal component analysis revealed that the lipid composition of the intestinal contents of red, black, and blue sea cucumbers differs from that of skin, and meats. Polyunsaturated fatty acids (PUFAs) are abundant in the intestinal contents, followed by meats of sea cucumber. Lipid nutritional quality assessments based on fatty acid composition revealed a high P:S ratio, low index of atherogenicity, and high health promotion indices for the intestinal contents of red sea cucumber, suggesting its potential health benefits. In addition, hierarchical cluster analysis revealed that the intestinal contents of sea cucumbers were relatively high in PUFA-enriched phospholipids and lysophospholipids. Ceramides are abundant in black skin, blue meat, and red intestinal content samples. Overall, this study provides the first insights into a comprehensive regio-specific profile of the lipid content of sea cucumbers and their potential use as a source of lipid nutrients in food and nutraceuticals.
• Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS

  Jayashankar Jayaprakash, Siddabasave Gowda B. Gowda, Pradeep K. Shukla, Divyavani Gowda, Lipsa Rani Nath, Hitoshi Chiba, Radhakrishna Rao, Shu-Ping Hui

  ACS Omega 2024/04/09
• Glucosylceramide flippases contribute to cellular glucosylceramide homeostasis

  Natsuki Kita, Asuka Hamamoto, Siddabasave Gowda B. Gowda, Hiroyuki Takatsu, Kazuhisa Nakayama, Makoto Arita, Shu-Ping Hui, Hye-Won Shin

  Journal of Lipid Research 2024/03
• Analysis of lipid composition and characterization of acyl steryl glycosides in rose petals by using nontargeted LC/MS

  Md Abdul Malek, Siddabasave Gowda B. Gowda, Divyavani Gowda, Shu-Ping Hui

  Journal of Food Measurement and Characterization 2024/03
• A facile method for monitoring sphingomyelin synthase activity in HeLa cells using liquid chromatography/mass spectrometry

  Punith M. Sundaraswamy, Yusuke Minami, Jayashankar Jayaprakash, Siddabasave Gowda B. Gowda, Hiroyuki Takatsu, Divyavani Gowda, Hye-Won Shin, Shu-Ping Hui

  The Analyst 2024
• Regiospecific analysis of lipidome in the brain from mammals of land and aquatic habitats-by liquid chromatography-mass spectrometry

  Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Chandra Shekhar, Hitoshi Chiba, Nina Patzke, Shu-Ping Hui

  Heliyon 9 (12) e22959 - e22959 2405-8440 2023/12
• Crosstalk between Lipids and Non-Alcoholic Fatty Liver Disease

  Divyavani Gowda, Chandra Shekhar, Siddabasave Gowda B. Gowda, Yifan Chen, Shu-Ping Hui

  Livers 3 (4) 687 - 708 2023/11/23 

  Non-alcoholic fatty liver disease (NAFLD), a complex liver disorder that can result in non-alcoholic steatohepatitis, cirrhosis, and liver cancer, is the accumulation of fat in the liver seen in people due to metabolic dysfunction. The pathophysiology of NAFLD is influenced by several variables, such as metabolic dysregulation, oxidative stress, inflammation, and genetic susceptibility. This illness seriously threatens global health because of its link to obesity, insulin resistance, type 2 diabetes, and other metabolic disorders. In recent years, lipid–NAFLD crosstalk has drawn a lot of interest. Through numerous methods, lipids have been connected to the onset and advancement of the illness. The connection between lipids and NAFLD is the main topic of the current review, along with the various therapeutic targets and currently available drugs. The importance of hepatic lipid metabolism in the progression of NAFLD is summarized with the latest results in the field.
• The elucidation of plasma lipidome profiles during severe influenza in a mouse model.

  Marumi Ohno, Siddabasave Gowda B Gowda, Toshiki Sekiya, Naoki Nomura, Masashi Shingai, Shu-Ping Hui, Hiroshi Kida

  Scientific reports 13 (1) 14210 - 14210 2023/08/30 

  Although influenza virus infection has been shown to affect lipid metabolism, details remain unknown. Therefore, we elucidated the kinetic lipid profiles of mice infected with different doses of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) by measuring multiple lipid molecular species using untargeted lipidomic analysis. C57BL/6 male mice were intranasally infected with PR8 virus at 50 or 500 plaque-forming units to cause sublethal or lethal influenza, respectively. Plasma and tissue samples were collected at 1, 3, and 6 days post-infection (dpi), and comprehensive lipidomic analysis was performed using high-performance liquid chromatography-linear trap quadrupole-Orbitrap mass spectrometry, as well as gene expression analyses. The most prominent feature of the lipid profile in lethally infected mice was the elevated plasma concentrations of phosphatidylethanolamines (PEs) containing polyunsaturated fatty acid (PUFA) at 3 dpi. Furthermore, the facilitation of PUFA-containing phospholipid production in the lungs, but not in the liver, was suggested by gene expression and lipidomic analysis of tissue samples. Given the increased plasma or serum levels of PUFA-containing PEs in patients with other viral infections, especially in severe cases, the elevation of these phospholipids in circulation could be a biomarker of infection and the severity of infectious diseases.
• Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS.

  Rachana M Gangadhara, Siddabasave Gowda B Gowda, Divyavani Gowda, Ken Inui, Shu-Ping Hui

  Foods (Basel, Switzerland) 12 (14) 2023/07/21 

  Beans, a globally significant economic and nutritional food crop, are rich in polyphenolic chemicals with potential health advantages, providing high protein, fiber, minerals, and vitamins. However, studies on the global profiling of lipids in beans are limited. We applied a non-targeted lipidomic approach based on high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (HPLC/LTQ-Orbitrap-MS) to comprehensively profile and compare the lipids in six distinct bean cultivars, namely, adzuki red beans-adzuki cultivar (ARB-AC), adzuki red beans-Benidainagon cultivar (ARB-BC), adzuki red beans-Erimoshouzu cultivar (ARB-EC), soybean-Fukuyutaka cultivar 2021 (SB-FC21), soybean-Fukuyutaka cultivar 2022 (SB-FC22), and soybean-Oosuzu cultivar (SB-OC). MS/MS analysis defined 144 molecular species from four main lipid groups. Multivariate principal component analysis indicated unique lipid compositions in the cultivars except for ARB-BC and ARB-EC. Evaluation of the concentrations of polyunsaturated fatty acid to saturated fatty acid ratio among all the cultivars showed that SB-FC21 and SB-FC22 had the highest value, suggesting they are the most beneficial for health. Furthermore, lipids such as acyl sterol glycosides were detected and characterized for the first time in these bean cultivars. Hierarchical cluster correlations revealed the predominance of ceramides in ARB-EC, lysophospholipids in SB-FC21, and glycerophospholipids in SB-OC. This study comprehensively investigated lipids and their compositions in beans, indicating their potential utility in the nutritional evaluation of beans as functional foods.
• Sleep deprivation in obesogenic setting alters lipidome and microbiome toward suboptimal inflammation in acute heart failure.

  Ganesh V Halade, Yusuf Mat, Siddabasave Gowda B Gowda, Shalini Jain, Shu-Ping Hui, Hariom Yadav, Vasundhara Kain

  FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37 (5) e22899  2023/05 

  Sleep is a fundamental medicine for cardiac homeostasis, and sleep-deprived individuals are prone to higher incidences of heart attack. The lipid-dense diet (obesogenic diet-OBD) is a cumulative risk factor for chronic inflammation in cardiovascular disease; thus, understanding how sleep fragmentation (SF) in an obesity setting impacts immune and cardiac health is an unmet medical need. We hypothesized whether the co-existence of SF with OBD dysregulates gut homeostasis and leukocyte-derived reparative/resolution mediators, thereby impairing cardiac repair. Two-month-old male C57BL/6J mice were randomized first into two groups, then four groups; Control, control + SF, OBD, and OBD + SF mice subjected to myocardial infarction (MI). OBD mice had higher levels of plasma linolenic acid with a decrease in eicosapentaenoic and docosahexaenoic acid. The OBD mice had lower Lactobacillus johnsonii indicating a loss of probiotic microbiota. SF in OBD mice increased Firmicutes/Bacteroidetes ratio indicative of a detrimental change in SF-directed microbiome. OBD + SF group increased in the neutrophil: lymphocyte ratio suggestive of suboptimal inflammation. As a result of SF, resolution mediators (RvD2, RvD3, RvD5, LXA4 , PD1, and MaR1) decreased and inflammatory mediators (PGD2 , PGE2 , PGF2a , 6k-PGF1a ) were increased in OBD mice post-MI. At the site of infarction, the proinflammatory cytokines Ccl2, IL1β, and IL-6 were amplified in OBD + SF indicating a robust proinflammatory milieu post-MI. Also, brain circadian genes (Bmal1, Clock) were downregulated in SF-subjected control mice, but remained elevated in OBD mice post-MI. SF superimposed on obesity dysregulated physiological inflammation and disrupted resolving response thereby impaired cardiac repair and signs of pathological inflammation.
• Mass spectrometric approaches in discovering lipid biomarkers for COVID-19 by lipidomics: Future challenges and perspectives.

  Siddabasave Gowda B Gowda, Chandra Shekhar, Divyavani Gowda, Yifan Chen, Hitoshi Chiba, Shu-Ping Hui

  Mass spectrometry reviews 2023/04/27 

  Coronavirus disease 2019 (COVID-19) has emerged as a global health threat and has rapidly spread worldwide. Significant changes in the lipid profile before and after COVID-19 confirmed the significance of lipid metabolism in regulating the response to viral infection. Therefore, understanding the role of lipid metabolism may facilitate the development of new therapeutics for COVID-19. Owing to their high sensitivity and accuracy, mass spectrometry (MS)-based methods are widely used for rapidly identifying and quantifying of thousands of lipid species present in a small amount of sample. To enhance the capabilities of MS for the qualitative and quantitative analysis of lipids, different platforms have been combined to cover a wide range of lipidomes with high sensitivity, specificity, and accuracy. Currently, MS-based technologies are being established as efficient methods for discovering potential diagnostic biomarkers for COVID-19 and related diseases. As the lipidome of the host cell is drastically affected by the viral replication process, investigating lipid profile alterations in patients with COVID-19 and targeting lipid metabolism pathways are considered to be crucial steps in host-directed drug targeting to develop better therapeutic strategies. This review summarizes various MS-based strategies that have been developed for lipidomic analyzes and biomarker discoveries to combat COVID-19 by integrating various other potential approaches using different human samples. Furthermore, this review discusses the challenges in using MS technologies and future perspectives in terms of drug discovery and diagnosis of COVID-19.
• Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease

  Asuka Hamamoto, Natsuki Kita, Siddabasave Gowda B Gowda, Hiroyuki Takatsu, Kazuhisa Nakayama, Makoto Arita, Shu-Ping Hui, Hye-Won Shin

  Cell Structure and Function 0386-7196 2023
• Temporal lipid profiling in the progression from acute to chronic heart failure in mice and ischemic human hearts

  Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Vibhu Parcha, Pankaj Arora, Ganesh V. Halade, Shu-Ping Hui

  Atherosclerosis 363 30 - 41 0021-9150 2022/12
• Detection and characterization of lipids in eleven species of fish by non-targeted liquid chromatography/mass spectrometry

  Siddabasave Gowda B. Gowda, Yusuke Minami, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui

  Food Chemistry 393 133402 - 133402 0308-8146 2022/11
• Analysis of Antioxidant Lipids in Five Species of Dietary Seaweeds by Liquid Chromatography/Mass Spectrometry

  Siddabasave Gowda B. Gowda, Chen Yifan, Divyavani Gowda, Yui Tsuboi, Hitoshi Chiba, Shu-Ping Hui

  Antioxidants 11 (8) 1538 - 1538 2022/08/08 

  Seaweeds are a good source of bioactive lipids and are known for their nutritional benefits, making them a valuable food source. Despite their dietary significance and nutritional importance, there are limited reports on comprehensive lipidome analysis of lipids with antioxidant properties. Therefore, this study aimed to compare the lipid profiles of five commonly consumed Japanese dietary seaweeds using non-targeted liquid chromatography/mass spectrometry (LC/MS). A total, of 304 molecular species from four major lipid classes were detected and characterized by MS/MS analysis. Multivariate statistical analysis revealed distinct lipid molecular compositions in kombu and sea mustard compared to hijiki, mozuku, and laver seaweeds. Kombu has been shown to contain large amounts of antioxidants, such as polyunsaturated fatty acids (PUFAs), and a high health promotion index compared to other seaweeds. Hierarchical cluster correlations indicated the predominance of glycerophospholipids (GPs) and glycerolipids (GLs) in sea mustard and kombu. As a result, dietary seaweeds have great potential as antioxidants and health-promoting foods for human consumption due to their high levels of PUFA-rich GPs and GLs. Unsaturated triacylglycerols are predominant in hijiki, whereas other health-beneficial lipids, such as monogalactosyldiacylglycerol and sulfoquinovosyl diacylglycerols, are predominant in sea mustard. This study provides a detailed characterization of lipids and their comparative fingerprints in seaweeds, demonstrating the potential use of dietary seaweeds in biotechnological and industrial applications involving the development of functional food products.
• Determination of short-chain fatty acids by N,N-dimethylethylenediamine derivatization combined with liquid chromatography/mass spectrometry and their implication in influenza virus infection.

  Divyavani Gowda, Yonghan Li, Siddabasave Gowda B Gowda, Marumi Ohno, Hitoshi Chiba, Shu-Ping Hui

  Analytical and bioanalytical chemistry 414 (22) 6419 - 6430 2022/07/16 

  Short-chain fatty acids (SCFAs) are the end products of the fermentation of complex carbohydrates by the gut microbiota. Although SCFAs are recognized as important markers to elucidate the link between gut health and disease, it has been difficult to analyze SCFAs with mass spectrometry technologies due to their poor ionization efficiency and high volatility. Here, we present a novel and sensitive method for the quantification of SCFAs, including C2-C6 SCFAs and their hydroxy derivatives, by liquid chromatography/tandem mass spectrometry (LC-MS/MS) upon N,N-dimethylethylenediamine (DMED) derivatization with a run time of 10 min. Moreover, the quantification method of DMED-derivatized SCFAs in intestinal contents using isotope-labeled internal standards was also established. The method validation was performed by analyzing spiked intestinal samples; the limits of detection and quantification of SCFAs with this method were found to be 0.5 and 5 fmol, respectively; the recovery was greater than 80% and good linearity (0.9932 to 0.9979) of calibration curves was obtained over the range from 0.005 to 5000 pmol/μL; the intraday and interday precisions were achieved in the range of 1-5%. Furthermore, the validated method was applied to analyze SCFAs in the cecum and colon contents of mice infected with the influenza virus. The results showed that the concentration of most of the SCFAs tested here decreased significantly in a time-dependent manner after the infection, suggesting a possibility that SCFAs in intestinal samples could be used as severe disease markers. Overall, we here successfully developed a simple, fast, and sensitive method for SCFA analysis by LC-MS/MS combined with DMED derivatization. The method for the quantification of SCFAs will be a useful tool for both basic research and clinical studies.
• Lipid fingerprinting of yellow mealwormTenebrio molitor by untargeted liquid chromatography-mass spectrometry

  S. G. B. Gowda, Y. Sasaki, E. Hasegawa, H. Chiba, S. P. Hui

  Journal of Insects as Food and Feed 8 (2) 157 - 168 2022/02/01 [Refereed]
   

  Insects such as Tenebrio molitor have been considered an alternative source of nutrition for animals and have also been adopted as human food throughout history, especially in Asia and Africa. Lipids are the second most abundant component followed by proteins. However, studies focusing on comprehensive lipid composition analsysis of these widely reared species are limited. The untargeted lipidomic analysis of yellow mealworm larvae (T. molitor) led to the identification of several lipid molecular species from lipid classes such as: free fatty acids, sphingolipids, phospholipids, and triacylglycerols. The results revealed that polyunsaturated fatty acids (PUFAs) (45%) are the most abundant fatty acids, followed by monounsaturated fatty acids (MUFAs) (42%) and saturated fatty acids (13%). Fatty acids such as FA 18:1 and FA 18:2 are the most abundant fatty acids and are substantially enriched in other complex lipids in the form of esters. Moreover, functional lipids such as sphingomyelins, ceramides, cardiolipins, phosphatidylinositols, and phosphatidylethanolamines were characterised for the first time, with a large number of MUFAs and PUFAs as their main acyl chains. Overall, our data showed the occurrence of multiple structurally diverse lipids in T. molitor, suggesting that mealworms are not only enriched with proteins but also have several functional lipids, which are highly beneficial to human and animal health. Thus, the larvae of T. molitor could serve as a promising candidate for the development of functional food and feed products.
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• Group IIA secreted phospholipase A2 controls skin carcinogenesis and psoriasis by shaping the gut microbiota

  Yoshimi Miki, Yoshitaka Taketomi, Yuh Kidoguchi, Kei Yamamoto, Kazuaki Muramatsu, Yasumasa Nishito, Jonguk Park, Koji Hosomi, Kenji Mizuguchi, Jun Kunisawa, Tomoyoshi Soga, Eric Boilard, Siddabasave Gowda B. Gowda, Kazutaka Ikeda, Makoto Arita, Makoto Murakami

  JCI Insight 7 (2) 2022/01/25 

  Besides promoting inflammation by mobilizing lipid mediators, group IIA secreted phospholipase A2 (sPLA2-IIA) prevents bacterial infection by degrading bacterial membranes. Here, we show that, despite the restricted intestinal expression of sPLA2-IIA in BALB/c mice, its genetic deletion leads to amelioration of cancer and exacerbation of psoriasis in distal skin. Intestinal expression of sPLA2-IIA is reduced after treatment with antibiotics or under germ-free conditions, suggesting its upregulation by gut microbiota. Metagenome, transcriptome, and metabolome analyses have revealed that sPLA2-IIA deficiency alters the gut microbiota, accompanied by notable changes in the intestinal expression of genes related to immunity and metabolism, as well as in the levels of various blood metabolites and fecal bacterial lipids, suggesting that sPLA2-IIA contributes to shaping of the gut microbiota. The skin phenotypes in Pla2g2a-/- mice are lost (a) when they are cohoused with littermate WT mice, resulting in the mixing of the microbiota between the genotypes, or (b) when they are housed in a more stringent pathogen-free facility, where Pla2g2a expression in WT mice is low and the gut microbial compositions in both genotypes are nearly identical. Thus, our results highlight a potentially new aspect of sPLA2-IIA as a modulator of gut microbiota, perturbation of which affects distal skin responses.
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• Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry

  Chongsheng Liang, Siddabasave Gowda B. Gowda, Divyavani Gowda, Toshihiro Sakurai, Iku Sazaki, Hitoshi Chiba, Shu-Ping Hui

  Antioxidants 11 (2) 229 - 229 2022/01/25 

  Lipid hydroperoxides (LOOH) are the initial products of the peroxidation of unsaturated lipids and play a crucial role in lipid oxidation due to their ability to decompose into free radicals and cause adverse effects on human health. Thus, LOOHs are commonly considered biomarkers of oxidative stress-associated pathological conditions. Despite their importance, the sensitive and selective analytical method for determination is limited, due to their low abundance, poor stability, and low ionizing efficiency. To overcome these limitations, in this study, we chemically synthesized eight fatty acid hydroperoxides (FAOOH), including FA 18:1-OOH, FA 18:2-OOH, FA 18:3-OOH, FA 20:4-OOH, FA 20:5-OOH, FA 22:1-OOH, FA 22:6-OOH as analytes, and FA 19:1-OOH as internal standard. Then, they were chemically labeled with 2-methoxypropene (2-MxP) to obtain FAOOMxP by one-step derivatization (for 10 min). A selected reaction monitoring assisted targeted analytical method was developed using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The MxP-labelling improved the stability and enhanced the ionization efficiency in positive mode. Application of reverse-phase chromatography allowed coelution of analytes and internal standards with a short analysis time of 6 min. The limit of detection and quantification for FAOOH ranged from 0.1–1 pmol/µL and 1–2.5 pmol/µL, respectively. The method was applied to profile total FAOOHs in chemically oxidized human serum samples (n = 5) and their fractions of low and high-density lipoproteins (n = 4). The linoleic acid hydroperoxide (FA 18:2-OOH) and oleic acid hydroperoxide (FA 18:1-OOH) were the most abundant FAOOHs in human serum and lipoproteins. Overall, our validated LC-MS/MS methodology features enhanced detection and rapid separation that enables facile quantitation of multiple FAOOHs, therefore providing a valuable tool for determining the level of lipid peroxidation with potential diagnostic applications.
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• Defining the kinetic effects of infection with influenza virus A/PR8/34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC/MS

  Divyavani Gowda, Marumi Ohno, Siddabasave Gowda B. Gowda, Hitoshi Chiba, Masashi Shingai, Hiroshi Kida, Shu-Ping Hui

  Scientific Reports 11 (1) 20161 - 20161 2021/12 

  Influenza remains a world-wide health concern, causing 290,000–600,000 deaths and up to 5 million cases of severe illnesses annually. Noticing the host factors that control biological responses, such as inflammatory cytokine secretion, to influenza virus infection is important for the development of novel drugs. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite and has essential biological functions in inflammation. However, the kinetic effects of influenza virus infection on physiological S1P levels and their signaling in multiple tissues remain unknown. In this study, we utilized a mouse model intranasally infected with 50 or 500 plaque forming units (PFU) of A/Puerto Rico/8/34 (H1N1; PR8) virus to investigate how S1P levels and expression of its regulating factors are affected by influenza virus infection by the liquid-chromatography/mass spectrometry and real-time PCR, respectively. The S1P level was significantly high in the plasma of mice infected with 500 PFU of the virus than that in control mice at 6 day-post-infection (dpi). Elevated gene expression of sphingosine kinase-1 (Sphk1), an S1P synthase, was observed in the liver, lung, white adipose tissue, heart, and aorta of infected mice. This could be responsible for the increased plasma S1P levels as well as the decrease in the hepatic S1P lyase (Sgpl1) gene in the infected mice. These results indicate modulation of S1P-signaling by influenza virus infection. Since S1P regulates inflammation and leukocyte migration, it must be worth trying to target this signaling to control influenza-associated symptoms.
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• Sphingosine-1-phosphate interactions in the spleen and heart reflect extent of cardiac repair in mice and failing human hearts

  SiddabasaveGowda B. Gowda, Divyavani Gowda, Vasundhara Kain, Hitoshi Chiba, Shu-Ping Hui, Charles E. Chalfant, Vibhu Parcha, Pankaj Arora, Ganesh V. Halade

  American Journal of Physiology-Heart and Circulatory Physiology 321 (3) H599 - H611 0363-6135 2021/09/01 [Refereed][Not invited]
   

  Previous studies indicate that sphingosine-1-phosphate (S1P) has some role in cardiovascular disease. This study adds quantitative and integrative systems-based approaches that are necessary for discovery and bedside translation. Here, we quantitated sphinganine, sphingosine, sphingosine-1-phosphate (S1P) in mice and human cardiac pathobiology. Interorgan S1P quantity and respective systems-based receptor activation suggest cardiac repair after myocardial infarction. Thus, S1P serves as a therapeutic target for cardiac protection in clinical translation.
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• Docosahexaenoic Acid Esters of Hydroxy Fatty Acid Is a Novel Activator of NRF2

  Siddabasave Gowda B. Gowda, Takayuki Tsukui, Hirotoshi Fuda, Yusuke Minami, Divyavani Gowda, Hitoshi Chiba, Shu-Ping Hui

  International Journal of Molecular Sciences 22 (14) 7598 - 7598 2021/07/15 [Refereed]
   

  Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with interesting physiological functions in mammals. Despite their structural diversity and links with nuclear factor erythroid 2-related factor 2 (NRF2) biosynthesis, FAHFAs are less explored as NRF2 activators. Herein, we examined for the first time the synthetic docosahexaenoic acid esters of 12-hydroxy stearic acid (12-DHAHSA) or oleic acid (12-DHAHOA) against NRF2 activation in cultured human hepatoma-derived cells (C3A). The effect of DHA-derived FAHFAs on lipid metabolism was explored by the nontargeted lipidomic analysis using liquid chromatography-mass spectrometry. Furthermore, their action on lipid droplet (LD) oxidation was investigated by the fluorescence imaging technique. The DHA-derived FAHFAs showed less cytotoxicity compared to their native fatty acids and activated the NRF2 in a dose-dependent pattern. Treatment of 12-DHAHOA with C3A cells upregulated the cellular triacylglycerol levels by 17-fold compared to the untreated group. Fluorescence imaging analysis also revealed the suppression of the degree of LDs oxidation upon treatment with 12-DHAHSA. Overall, these results suggest that DHA-derived FAHFAs as novel and potent activators of NRF2 with plausible antioxidant function.
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• Detection and Structural Characterization of SFAHFA Homologous Series in Mouse Colon Contents by LTQ-Orbitrap-MS and Their Implication in Influenza Virus Infection

  Siddabasave Gowda B. Gowda, Divyavani Gowda, Marumi Ohno, Chongsheng Liang, Hitoshi Chiba, Shu-Ping Hui

  Journal of the American Society for Mass Spectrometry 32 (8) 2196 - 2205 1044-0305 2021/06/25 [Refereed][Not invited]
   

  Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with promising physiological functions in mammals. We previously introduced a new type of lipids to this family called short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), branching specific to the C2 carbon of a long-chain fatty acid (≥C20). In this study, we discovered a homologous series of SFAHFAs comprising C16-C26 hydroxy fatty acids esterified with short-chain fatty acids (C2-C5) in mouse colon contents. The detected SFAHFAs were characterized by high-resolution mass spectrometry with MSn analysis. The double-bond position of monounsaturated SFAHFAs was determined by the epoxidation reaction of samples with m-chloroperoxybenzoic acid and their MSn analysis. Further, the measurement of SFAHFA concentration in the colon contents of mice infected with influenza A/Puerto Rico/8/34 (H1N1; PR8) virus revealed a significant increase in their levels compared to native control. A strong correlation was observed between hydroxy fatty acid and SFAHFAs. Detection, characterization, and profiling of these new SFAHFA levels in relation with pandemic H1N1; PR8 influenza virus will contribute to the in-depth study of their function and metabolism.
  ダウンロード
• Lipidomic analysis of non-esterified furan fatty acids and fatty acid compositions in dietary shellfish and salmon by UHPLC/LTQ-Orbitrap-MS

  Siddabasave Gowda B. Gowda, Yusuke Minami, Divyavani Gowda, Daisuke Furuko, Hitoshi Chiba, Shu-Ping Hui

  Food Research International 144 110325 - 110325 0963-9969 2021/03 [Refereed][Not invited]
   

  Lipids such as furan fatty acids (F-acids) are the valuable minor bioactive components of food such as fatty fish and plants. They are reported to have positive health benefits, including antioxidant and anti-inflammatory activities. Despite their importance, limited studies are focusing on F-acid determination in dietary seafood. This study aimed to identify and profile non-esterified F-acids and free fatty acids in total lipid extract of seafood such as shellfish and salmon. The lipidomic analysis using liquid chromatography-linear trap quadrupole-orbitrap mass spectrometry led to identifying seven types of free F-acids in shellfish (n = 5) and salmon (n = 4). The identified F-acids were confirmed by their high-resolution masses and acquired mass spectra. The relative concentrations of F-acids in shellfish range from 0.01 to 10.93 mg/100 g of the fillet, and in salmon, 0.01 to 14.21 mg/100 g of the fillet. The results revealed the highest abundance of F-acids in Sakhalin surf clam, Japanese scallop, and a fatty salmon trout. Besides, relative levels of saturated, monounsaturated, and polyunsaturated fatty acids (PUFAs) in these seafoods were compared with each other, suggesting basket clams and salmon trout to have significantly higher levels of PUFAs. The dietary seafoods enriched with F-acids and PUFAs may have possible health benefits. Hence, the applied technique could be a promising tool for rapid detection and analysis of non-esterified fatty acids in food.
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• Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry

  Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Takayuki Furukawa, Siddabasave Gowda B. Gowda, Yue Wu, Kazuhiro Nouso, Yuki Fujii, Yuki Yoshikawa, Hitoshi Chiba, Shu-Ping Hui

  Analytical and Bioanalytical Chemistry 1618-2642 2020/10/22 [Refereed][Not invited]
  
  ダウンロード
• Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents

  Siddabasave Gowda B. Gowda, Divyavani Gowda, Chongsheng Liang, Yonghan Li, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu-Ping Hui

  Metabolites 10 (10) 398 - 398 2020/10/08 [Refereed][Not invited]
   

  Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.
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• Identification of short‐chain fatty acid esters of hydroxy fatty acids (SFAHFAs) in a murine model by nontargeted analysis using ultra‐high‐performance liquid chromatography/linear ion trap quadrupole‐Orbitrap mass spectrometry

  Siddabasave Gowda B. Gowda, Chongsheng Liang, Divyavani Gowda, Fengjue Hou, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, Shu‐Ping Hui

  Rapid Communications in Mass Spectrometry 34 (17) 0951-4198 2020/09/15 [Refereed][Not invited]
  
  ダウンロード
• Untargeted Lipidomic Analysis of Plasma from High-fat Diet-induced Obese Rats Using UHPLC–Linear Trap Quadrupole–Orbitrap MS

  Siddabasave Gowda B. GOWDA, Zi-Jun GAO, Zhen CHEN, Takayuki ABE, Shota HORI, Satoru FUKIYA, Satoshi ISHIZUKA, Atsushi YOKOTA, Hitoshi CHIBA, Shu-Ping HUI

  Analytical Sciences 36 (7) 821 - 828 0910-6340 2020/07/10 [Refereed][Not invited]
  
  ダウンロード
• A Simple and Efficient Method for Synthesis of sn ‐Glycero ‐Phosphoethanolamine

  Siddabasave Gowda B. Gowda, Hirotoshi Fuda, Yusuke Yamamoto, Hitoshi Chiba, Shu‐Ping Hui

  Lipids 55 (4) 395 - 401 0024-4201 2020/07 [Refereed][Not invited]
  
  ダウンロード
• Discovery of Eicosapentaenoic Acid Esters of Hydroxy Fatty Acids as Potent Nrf2 Activators

  Siddabasave Gowda B. Gowda, Hirotoshi Fuda, Takayuki Tsukui, Hitoshi Chiba, Shu-Ping Hui

  Antioxidants 9 (5) 397 - 397 2020/05/08 [Refereed][Not invited]
   

  Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids with anti-inflammatory and anti-diabetic properties. Despite the possible link between endogenous FAHFA levels and nuclear factor erythroid 2-related factor 2 (Nrf2), their possible function as antioxidants and the mechanisms involved in this are unknown. Here, we investigate FAHFAs’ plausible antioxidant potential with reference to their effect on the Nrf2 levels, oxidative stress, and lipid droplet oxidation in human hepatocytes (C3A). Six authentic FAHFAs were chemically synthesized and performed activity-based screening by reporter gene assay. Among them, eicosapentaenoic acid (EPA) esterified 12-hydroxy stearic acid (12-HSA) and 12-hydroxy oleic acid (12-HOA) FAHFAs showed less cytotoxicity compared to their free fatty acids and potent activators of Nrf2. To define their mode of action, relative levels of nuclear Nrf2 were determined, which found a higher amount of Nrf2 in nucleus of cells treated with 12-EPAHSA compared to the control. Furthermore, 12-EPAHSA increased the expression of Nrf2-dependent antioxidant enzyme genes (NQO1, GCLM, GCLC, SOD-1, and HO-1). Fluorescence imaging analysis of linoleic-acid-induced lipid droplets (LDs) in C3A cells treated with 12-EPAHSA revealed the strong inhibition of small-size LD oxidation. These results suggest that EPA-derived FAHFAs as a new class of lipids with less cytotoxicity, and strong Nrf2 activators with plausible antioxidant effects via the induction of cytoprotective proteins against oxidative stress, induced cellular damage.
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• Facile Chemoselective Strategy toward Capturing Sphingoid Bases by a Unique Glutaraldehyde-Functionalized Resin.

  Siddabasave Gowda B Gowda, Atsufumi Nakahashi, Keiko Yamane, Saori Nakahashi, Yuta Murai, Ananda Kumar C Siddegowda, Mostafa A S Hammam, Kenji Monde

  ACS omega 3 (1) 753 - 759 2018/01/31 [Refereed][Not invited]
   

  Sphingoid bases, which have a 2-amino-1,3-diol common functional group, are the structural backbone units of all sphingolipids. Recently, much attention has been focused on sphingoid bases because of their potentially beneficial bioactivities toward various cancer cells as well as their dietary interest. However, low abundance and the handling complexity caused by their amphiphilic character led to very limited research on them. Glutaraldehyde has two aldehyde groups, and it reacts rapidly with the 2-amino-1,3-diol functional group of sphingosine to give a tricyclic product. Immobilization of glutaraldehyde on a resin was successfully performed by organic synthesis, starting from trans-p-coumaric acid via eight steps. This approach suppresses the self-polymerization of glutaraldehyde, and addition of water to the developed resin causes the formation of cyclic double hemiacetal function, which avoids oxidation like a reducing sugar in nature and makes it stable even for up to 1 year incubation. The resin was applied to the solid-phase extracting experiment of free sphingosine from human serum at a concentration of 280 nM. Another extraction study of edible golden oyster mushrooms showed that the sphingoid base was selectively captured from complex natural extracts. These results demonstrate that the developed glutaraldehyde resin method is a highly selective method, and hence, the combination of it with the o-phthaldialdehyde HPLC method was confirmed as an efficient and sensitive method for analysis of sphingoid bases in biological samples.
  ダウンロード
• “Facile determination of sphingolipids under alkali conditions using metal free column by LC-MS/M

  BOMMEGOWDA SIDDABASAVEGOWDA

  Analytical Bioanalytical chemistry 410 (20) 4793 - 4803 2018 [Refereed][Not invited]
   

  Extraction and analysis of sphingolipids from biological samples is a critical step in lipidomics, especially for minor species such as sphingoid bases and sphingosine-1-phosphate. Although several liquid chromatography-mass spectrometry methods enabling the determination of sphingolipid molecular species have been reported, they were limited in analytical sensitivity and reproducibility by causing significant peak tailing, especially by the presence of phosphate groups, and most of the extraction techniques are laborious and do not cover a broad range of sphingolipid metabolites. In this study, we developed a rapid single-phase extraction and highly sensitive analytical method for the detection and quantification of sphingolipids (including phosphates) comprehensively using liquid chromatography-triple quadruple mass spectrometry. After validating the reliability of the method, we analyzed the intestinal tissue sphingolipids of germ-free (GF) and specific pathogen-free (SPF) mice and found significantly higher levels of free sphingoid bases and sphingosine-1-phosphate in the GF condition as compared to the SPF condition. This method enables a rapid extraction and highly sensitive determination of sphingolipids comprehensively at low femtomolar ranges. Graphical abstract Diagrammatic comparision of sphingolipid (phosphates) analysis between conventional and this method.
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• Stereochemical Study of Sphingosine by Vibrational Circular Dichroism

  Atsufumi Nakahashi, Ananda Kumar C. Siddegowda, Mostafa A. S. Hammam, Siddabasave Gowda B. Gowda, Yuta Murai, Kenji Monde

  ORGANIC LETTERS 18 (10) 2327 - 2330 1523-7060 2016/05 [Refereed][Not invited]
   

  Vibrational circular dichroism (VCD) was first applied to the stereochemical analysis of sphingosine. VCD patterns derived from the C=C stretch as well as other mid-infrared (IR) regions were practical markers to discriminate all the stereoisomers of intact sphingosine. Glutaraldehyde was found as an excellent derivatizing reagent for sphingosine which improves its solubility in VCD-friendly nonpolar solvents such as chloroform and enhances the VCD intensities by forming a rigid cyclized structure.
  ダウンロード
• Highly efficient preparation of sphingoid bases from glucosylceramides by chemoenzymatic method

  Siddabasave B. Gowda Gowda, Seigo Usuki, Mostafa A. S. Hammam, Yuta Murai, Yasuyuki Igarashi, Kenji Monde

  JOURNAL OF LIPID RESEARCH 57 (2) 325 - 331 0022-2275 2016/02 [Refereed][Not invited]
   

  Sphingoid base derivatives have attracted increasing attention as promising chemotherapeutic candidates against lifestyle diseases such as diabetes and cancer. Natural sphingoid bases can be a potential resource instead of those derived by time-consuming total organic synthesis. In particular, glucosylceramides (GlcCers) in food plants are enriched sources of sphingoid bases, differing from those of animals. Several chemical methodologies to transform GlcCers to sphingoid bases have already investigated; however, these conventional methods using acid or alkaline hydrolysis are not efficient due to poor reaction yield, producing complex by-products and resulting in separation problems. In this study, an extremely efficient and practical chemoenzymatic transformation method has been developed using microwave-enhanced butanolysis of GlcCers and a large amount of readily available almond beta-glucosidase for its deglycosylation reaction of lysoGlcCers. The method is superior to conventional acid/base hydrolysis methods in its rapidity and its reaction cleanness (no isomerization, no rearrangement) with excellent overall yield.

Books etc

• Advances in Nanocomposite Materials for Environmental and Energy Harvesting Applications

  (Joint workCarbon Nanostructure Based Composites for Environmental and Energy Applications)
  Springer nature 2022 (ISBN: 9783030943196)

Conference Activities & Talks

• Lipid kinetics from Acute to Chronic Heart failure in mice and Ischemic Human hearts.

  Siddabasave Gowda, B. Gowda, Gowda D, Chiba H, Parcha V, Arora P, Halade G, Hui S-P

  17th Bioactive lipids in Cancer, Inflammation and Related Diseases, Oct-30- Nov. 02, 2022, New Orleans, USA.
• A facile analytical method for determination of Short-Chain Fatty Acid Esters of Hydroxy Fatty Acids

  Fengiue H, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P

  第17回がん・炎症・関連疾患における生理活性脂質、2022年10月30日-11月02日、米国ニューオリンズ。
• Investigating effect of Kasokero virus infection on lipid metabolism

  Siddabasave Gowda, B. Gowda, Kajihara M, Ohno M

  第8回北海道大学部局横断シンポジウム、2022年、10月28日
• Analysis of lipid biomarkers for heart failure by LC/MS

  Siddabasave gowda, B. Gowda

  5th Annual Meeting / 2nd Neutral Fat Month, Oct 15, 2022, Fukuoka, Japan
• Obesity a lifestyle disorder of global health concern“  [Invited]

  Siddabasave Gowda, B. Gowda

  2nd International Conference United Nations World Peace Association 2022, October 09-11, Sapporo, Japan.
• “Analysis of Lipids Characterization in five species of dietary seaweeds by liquid chromatography/mass spectrometry“

  Chen Y, Hui S-P, Chiba H, D, Gowda, Siddabasave Gowda, B. Gowda

  The 61st Annual Meeting of the Japanese Society of Clinical Chemistry
• Analysis of Short Chain Fatty Acid Esters of Hydroxy Fatty Acids by Liquid Chromatography/Mass Spectrometry“

  Li Y, Siddabasave Gowda, B. Gowda, D, Gowda, Hui S-P, Chiba H

  The 61st Annual Meeting of the Japanese Society of Clinical Chemistry
• “Influenza virus A / PR8 / 34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC / MS“

  D., Gowda, Chiba H, Hui S-P, Siddabasave Gowda, B. Gowda

  The 61st Annual Meeting of the Japanese Society of Clinical Chemistry
• “Lipid biomarker analysis for influenza virus infection by liquid chromatography-mass spectrometry“

  Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P

  The 61st Annual Meeting of the Japanese Society of Clinical Chemistry
• "Discovery of Food-Based Inhibitors of Obesity-Related Sphingolipid Metabolism"

  Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P

  The 61st Annual Meeting of the Japanese Society of Clinical Chemistry
• “Synthesis and Development of a Facile Analytical Method for Short Chain Fatty Acid Esters of Hydroxy Fatty Acids“  [Not invited]

  Hou F, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P

  The 47th Annual Meeting of the Japanese Society for Medical Mass Spectrometry
• Tackling Unknown Lipids in Eleven Dietary Fish and Their Composition Analysis by Untargeted LC/MS“

  Siddabasave Gowda, B. Gowda, Minami Y, D., Gowda, Chiba H, Hui S-P

  The 47th Annual Meeting of the Japanese Society for Medical Mass Spectrometry.
• “Discovery of Food Derived Inhibitor for Sphingolipid Metabolism Associated to Obesity"  [Not invited]

  Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P

  The 47th Annual Meeting of the Japanese Society for Medical Mass Spectrometry
• Lipid fingerprinting in eleven dietary fish by untargeted LC/MS  [Invited]

  Siddabasave Gowda, B. Gowda, Minami Y, Gowda D, Chiba H, Hui S-P

  6th International Symposium on Phytochemicals in Medicine and Food  2022/08
  Download
• Exploring Functional Lipids in Food, Health, and Disease by state-of-the-art of mass spectrometry

  Siddabasave gowda, Bomme Gowda

  Two days Virtual International Conference on Advanced Materials, Dept. of Organic Chemistry, University of Mysore, India.  2022/03
  Download
• Kinetic profile of lipids from acute to chronic myocardial infarction

  Siddabasave Gowda, B. Gowda, Gowda D, Chiba H, Hui S-P

  The 60th Annual Meeting of the Japanese Society of Clinical Chemistry  2021/11
• Chemistry of Functional Lipids in Health and Disease  [Invited]

  Siddabasave gowda, Bomme Gowda

  A Virtual Conference on Recent Advances in Chemical Science and Medicinal Chemistry,JSS Science & Technology University, India  2021/07
  Download
• Facile determination of lipid hydroperoxides by one-step derivatization coupled targeted LC-MS/MS

  Chongsheng Liang, Siddabasavegowda, B. Gowda, Hitoshi Chiba, Shu-ping Hui

  The 60th Annual Meeting of the Japanese Society of Clinical Chemistry
  Download
• Role of Sphingosine -1-phosphate signalling in myocardial infarction

  Siddabasave Gowda, B. Gowda, Divayvani Gowda, Hitoshi Chiba, Ganesh Halade, Shu-ping Hui

  The 60th Annual Meeting of the Japanese Society of Clinical Chemistry
  Download
• “Discovery of n-3 fatty acid derived lipids as potent Nrf2 mediated antioxidants”

  Hitoshi Chiba, Siddabasavegowda, B. Gowda, Hirotoshi Fuda, Takayuki Tsukui, Shu-Ping Hui

  The 60th Annual Meeting of the Japanese Society of Clinical Chemistry
  Download
• Global lipid fingerprinting for biomarker discovery and their functional studies

  Siddabasave gowda B. Gowda

  第6回 北大・部局横断シンポジウム
• Uncover the role of lipids in health and disease by lipidomics.

  Siddabasave Gowda, B. Gowda

  超異分野meetup- 2020 Sapporo, Japan (札幌，日本)
• Improved method for analysis of sphingolipids by ultra-high performance liquid chromatography- mass spectrometry”2nd Japan-Korea Lipid symposium  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  2nd Japan-Korea Lipid symposium  2018/09
• Application of novel polymer sustained glutaraldehyde resin towards rapid extraction of sphigolipids  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  Japan-Taiwan Bilateral polymer symposium  2015/09
• A novel approach towards efficient supply of natural sphingoid bases and their application as multi drug resistance inhibitors in cancer  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  14th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases  2015/07
• Development of novel extraction method of natural sphingoids by chemoselective capturing reaction  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  27th Banyu Sapporo Symposium  2015/07
• Application of novel polymer sustained glutaraldehyde resin towards rapid extraction of sphingoids  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  64th SPSJ Annual Meeting  2015/05
• Innovative tactic towards rapid isolation of unique bioactive sphingoids from dietary natural resources  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  249th ACS National Meeting & Exposition  2015/03
• Development of a highly efficient novel methodology for the rapid isolation of bioactive sphingoid base – toward construction of their chemical library  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  The 2nd International Life-Science Symposium for Young Scientists  2014/10
• Development of a highly efficient novel methodology for the isolation of sphingoid bases and its application towards construction of their chemical library  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  The 12th International Symposium for future Drug Discovery and Medical Care  2014/09
• Chemoselective capture of sphingolipids by efficient resin and stereochemical analysis method  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  Molecular Chirality 2014  2014/06
• A new tactic towards rapid extraction of unique sphingoid bases  [Not invited]

  BOMMEGOWDA SIDDABASAVEGOWDA

  Chemical Society Japan Meeting  2014/01
• Short-chain fatty acid esters of hydroxy fatty acids - a new gut lipid biomarkers for obesity and influenza infection

  SIDDABASAVEGOWDA B. GOWDA, CHONGSHENG LIANG, DIVYAVANI GOWDA, FENGJUE HOU, HITOSHI CHIBU, SHU-PING HUI
• Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents

  i Y, D, Gowda, Siddabasave Gowda, B. Gowda, Liang C, Chiba H, Hui S-P

  The 61stAnnual Meeting of the Japanese Society of Clinical Chemistry
• Global lipid fingerprinting for early disease biomarker discovery by LC/MS

  46th Japan Society for Biomedical Mass Spectrometry
• Lipid fingerprinting for lipid biomarker discovery of drug-resistance in leukemia

  Matsuhisa C, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S.P

  46th Japan Society for Biomedical Mass Spectrometry
• Comprehensive analysis of lipid composition in dietary Japanese fish using UHPLC/LTQ Orbitrap MS

  Minami Y, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P

  46th Japan Society for Biomedical Mass Spectrometry
• Synthesis and Development of a Facile Analytical Method for Short-Chain Fatty Acid Esters of Hydroxy Fatty Acids

  Fengiue H, Siddabasave Gowda, B. Gowda, D., Gowda, Chiba H, Hui S-P

  46th Japan Society for Biomedical Mass Spectrometry
• One-step derivatization assisted targeted analysis of fatty acid hydroperoxide by LC/MS  [Not invited]

  Liang C, Siddabasave Gowda B, Sakurai T, Chiba H, Hui S.P

  46th Japan Society for Biomedical Mass Spectrometry
• Saturated fatty acid esters of hydroxy fatty acids as novel biomarkers of influenza virus infection

  Siddabasave Gowda, B. Gowda, D., Gowda, Oono M, Liang C, Chiba H, Hui S-P

  Japan Society for Clinical chemistry Hokkaido branch Meeting 55th Sapporo, Japan
• Discovery of short chain fatty acid esters of hydroxy fatty acid in intestinal contents as novel biomarkers for obesity

  Japan Society for Clinical chemistry Hokkaido branch Meeting Sapporo, Japan
• Untargeted lipidomic analysis reveals new lipid biomarkers for myocardial infarction

  Siddabasave gowda, B. Gowda

  第54回日本臨床検査医 学会・第30回日本臨床 化学会北海道支部例会 (誌上開催)(札幌，日 本)

MISC

• Comprehensive analysis of lipid composition in the dietary Japanese fish using UHPLC/LTQ Orbitrap MS

  MINAMI Yusuke, BOMMEGOWDA Siddabasavegowda, GOWDA Divyavani, CHIBA Hitoshi, HUI Shu-Ping  JSBMS Letters  46-  (Supplement)  2021
• Lipid fingerprinting for lipid biomarker discovery of drug-resistance in leukemia

  MATSUHISA Chihiro, BOMMEGOWDA Siddabasavegowda, USUKI Seigo, SAKURAI Toshihiro, CHIBA Hitoshi, HUI Shu-Ping  JSBMS Letters  46-  (Supplement)  2021
• One-step derivatization assisted targeted analysis of fatty acid hydroperoxides by LC/MS.

  LIANG Chongsheng, BOMMEGOWDA Siddabasavegowda, SAKURAI Toshihiro, CHIBA Hitoshi, HUI Shu-Ping  JSBMS Letters  46-  (Supplement)  2021
• Global lipid fingerprinting for early disease biomarker discovery by LC/MS

  BOMMEGOWDA Siddabasavegowda, GOWDA Divyavani, LIANG Chongsheng, CHIBA Hitoshi, HUI Shu-Ping  JSBMS Letters  46-  (Supplement)  2021
• Synthesis and Development of a Facile Analytical Method for Short Chain Fatty Acid Esters of Hydroxy Fatty Acids

  HOU Fengjue Hou, BOMMEGOWDA Siddabasavegowda, GOWDA Divyavani, CHIBA Hitoshi, HUI Shu-Ping  JSBMS Letters  46-  (Supplement)  2021

Industrial Property Rights

• 特許P2016-79161A:糖スフィンゴシン及びスフィンゴ塩基の製造方法発明者    2016/05/16

  BOMMEGOWDA SIDDABASAVEGOWDA

Awards & Honors

• 2022/08 Hokkaido University FY2022 Support System for the Collaborative Research of Next-Generation Researchers:
   Investigating the role of Shishamo-a native fish of Hokkaido in Obesity control 

  受賞者: Siddabasave gowda;Bomme gowda
• 2022/07 Hokkaido University FY2022 SOUSEI Support Program for Young Researcher Grant
   Tackling Obesity Associated to Lipid metabolism with Food Bioactives 

  受賞者: Siddabasa gowda;Bomme gowda
• 2022/03 2020-2021 Top cited author from the Rapid Communications in Mass spectrometry
  
• 2021/11 Hokkaido University Hokkaido Unviversity-Cross-departmental Symposium / Grant-Bronze Award
   Investigation of role of new gut biomarkers of influenza infection 

  受賞者: Siddabasave gowda;Bomme Gowda
• 2021/06 Hokkaido University FY2021 Support System for the Collaborative Research of Next-Generation Researchers:
   “Unrevealing the links between Kombu and obesity associated to sphingolipid metabolism“ 

  受賞者: Siddabasave gowda;Bomme Gowda
• 2021/04 JSPS Early Carrier Scientist
   Exploring the functional role of Hijiki in obesity-associated to sphingolipid metabolism 

  受賞者: Siddabasavegowda;Bomme gowda
• 2020/06 Hokkaido University, Faculty of Health Sciences Start-up grant
  
• 2017/12 TBRF, Japan Postdoctoral fellowship
   TBRF Postdoctoral Fellow (declined) 

  受賞者: BOMMEGOWDA SIDDABASAVEGOWDA
• 2016/02 JASSO, Japan Scholarship
   PhD scholar 

  受賞者: BOMMEGOWDA SIDDABASAVEGOWDA
• 2015/07 ONO Pharmacetical Travel Award
   14th Bioactive lipid conference, Hungary 

  受賞者: BOMMEGOWDA SIDDABASAVEGOWDA
• 2015/03 Hokkaido University Travel Award
   249th ACS meeting, Colorado, USA 

  受賞者: BOMMEGOWDA SIDDABASAVEGOWDA
• 2012/10 Japanese governament IGP-MEXT Scholarship
   Phd Research fellow 

  受賞者: BOMMEGOWDA SIDDABASAVEGOWDA

Research Grants & Projects

• "Exploring the functional role of Hijiki in obesity-associated to sphingolipid metabolism"

  JSPS：Early Carrier Scientist Award

  Date (from‐to) : 2021/04 -2025/03
• Investigating the role of Shishamo-a native fish of Hokkaido in Obesity control

  Hokkaido University：FY2022 Support System for the Collaborative Research of Next-Generation Researchers:

  Date (from‐to) : 2022/08 -2023/03 

  Author : Siddabasave gowda, Bomme Gowda
• Tackling Obesity Associated to Lipid metabolism with Food Bioactives

  Hokkaido University：FY2022 SOUSEI Support Program for Young Researcher Grant

  Date (from‐to) : 2022/07 -2023/03 

  Author : Siddabasave gowda, Bomme Gowda
• Investigation of role of new gut biomarkers of influenza infection

  Hokkaido University：The 7th Hokkaido University Cross-Departmental Symposium Research Grant

  Date (from‐to) : 2021/11 -2022/03 

  Author : Siddabasave gowda, Bomme Gowda
• "Unrevealing the links between Kombu and obesity associated to sphingolipid metabolism"

  Hokkaido University：FY2021 Support System for the Collaborative Research of Next-Generation Researchers:

  Date (from‐to) : 2021/07 -2022/02
• Start-up grant of 300,000 yen

  Hokkaido University：

  Date (from‐to) : 2019/06 -2021/05 

  Author : BOMMEGOWDA SIDDABASAVEGOWDA

Educational Activities

Teaching Experience

Committee Membership

• 2023/04 - Today   Student Committee International Section Members
• 2022/04 -2023/03   Faculty of Health Science, Hokkaido University   Social gathering committee
• 2022/04 -2023/03   Graduate School of Global Food Resources Hokkaido University   Entrance Examination committee
• 2022/04 -2023/03   Faculty of Health Science, Hokkaido University   Faculty development (FD) committee
• 2021/04 -2022/03   Graduate School of Global Food Resources Hokkaido University   Examination evaluation committee
• 2021/04 -2022/03   Faculty of Health Science, Hokkaido University   Fellowship secretary
• 2021/04 -2022/03   Graduate School of Global Food Resources Hokkaido University   Public Lecture Committee
• 2020/04 -2021/03   Faculty of Health Science   Open Lecture Commite

Social Contribution

Social Contribution

Media Coverage

• https://www.global.hokudai.ac.jp/blog/lipids-with-potential-health-benefits-in-herbal-teas/
• Sphingosine-1-phosphate interactions in the spleen and heart reflect extent of cardiac repair in mice and failing human hearts

  Writer: Other than myself

  Publisher, broadcasting station: for details access this link: https://physiology.altmetric.com/details/112226626/news

  Paper

Academic Contribution

• National and International Collaboration activities

  Role: Others

  Type: Academic research

  Organizer, responsible person: with Prof. Ganesh Halade, University of South Florida, USA; Dr. Nina Patzke, Heath and Medical University, Germany; Prof. Hye-Won Shin, Kyoto University, Japan; Prof. Hiroshi Kida, Dr. Ohno, International Institute for Zoonosis control, Hokkaido University; Prof. Atsushi Yokota, Faculty of Agriculture, Hokkaido University. 8. Dr. Yaser Hosny Ali Elewa, Faculty of veterinary Medicine, Hokkaido University; Dr. Takayuki Tsukui, Sapporo University of Health Sciences.
• Reviewer for various journals

  Role: Peer review

  Type: Peer review etc

  Organizer, responsible person: • Food Control (Elsevier) • Phytochemical Analysis (Wiley) • NPJ Science of Food (Springer) • Molecules, Metabolites, Microorganisms, Energies, & IJMS (MDPI)