Researcher Database
Last Updated :2024/11/16
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Profile and Settings
Degree
Profile and Settings
Name (Japanese)
YamakiName (Kana)
ShogoName
GOE-3655-2022, 201801002643573670
Achievement
Research Interests
Research Areas
- Life sciences / Marine/Aquatic life sciences
- Life sciences / Applied microbiology
- Life sciences / Food sciences
Research Experience
- 2018/04 - Today 北海道大学大学院水産科学研究院 海洋応用生命科学部門 助教
- 2017/04 - 2018/03 北海道大学大学院水産科学研究院 日本学術振興会特別研究員 PD（DC2より切替）
- 2016/04 - 2017/03 北海道大学大学院水産科学院 日本学術振興会特別研究員 DC2
Education
- 2014/04 - 2017/03 Hokkaido University Graduate School of Fisheries Sciences
- 2012/04 - 2014/03 北海道大学
- 2008/04 - 2012/03 Hokkaido University School of Fisheries Sciences
- 2005/04 - 2008/03 北海道大麻高等学校
Awards
- 2024/01 公益社団法人 日本水産学会 日本水産学会 令和5年度北海道支部大会 最優秀講演賞（発表者）
腸炎ビブリオに感染するバクテリオファージVF16 の性状受賞者: 三浦太暉;⼭⽊将悟;⽥渕いつき;⼭﨑浩司;川合祐史 - 2023/11 Japan Canners Association Henmi Award
Inhibition of Morganella morganii growth and histamine production using a bacteriophage cocktail受賞者: Shogo Yamaki;Aoi Sakanoue;Kosuke Arai;Koji Yamazaki;Yuji Kawai - 2023/08 The Japanese Society for Food Science and Technology Food Science and Technology Research Award Vol. 28
Inhibition of Morganella morganii growth and histamine production using a bacteriophage cocktail受賞者: Shogo Yamaki;Aoi Sakanoue;Kosuke Arai;Koji Yamazaki;Yuji Kawai - 2022/05 The Society for Antibacterial and Antifungal Agents, Japan 令和3年度 日本防菌防黴学会 論文賞（共著者）
Spore heat resistance and growth ability at refrigeration temperatures of Bacillus spp. and Paenibacillus spp.受賞者: 小林哲也;東考憲;八十川大輔;山木将悟;山﨑浩司 - 2018/09 Japanese Society of Food Microbiology Japanese Journal of Food Microbiology 2017 Best Paper Award
Distribution of fishery products and histamine producing ability of Morganella psychrotolerans, a psychrotrophic histamine-producing bacterium.受賞者: Riko Kato;Di Wang;Shogo Yamaki;Yuji Kawai;Koji Yamazaki - 2017/03 北海道大学大学院水産科学院 伊藤一隆賞
- 2015/08 日本食品科学工学会 第11回若手の会 優秀ポスター企業賞
広宿主域を示すPhotobacterium damselae subsp. damselae溶菌バクテリオファージの探索とその性状受賞者: 山木将悟;黒沼宗弥;久保田光矢;川合祐史;山崎浩司
Published Papers
- Daiki Miura, Shogo Yamaki, Itsuki Tabuchi, Yuji Kawai, Koji YamazakiApplied Food Research 4 (2) 100558 2772-5022 2024/12 [Refereed][Not invited]
- Shuya Hatakeyama, Sayaka Mino, Mana Mizobata, Mako Takada, Jiro Tsuchiya, Shogo Yamaki, Yasuhiro Ando, Tomoo Sawabe, Ken TakaiInternational Journal of Systematic and Evolutionary Microbiology 74 (10) 006553 1466-5026 2024/10/22 [Refereed]
A novel mesophilic bacterium, strain SS33T, was isolated from a deep-sea hydrothermal vent chimney at Suiyo Seamount, Izu-Bonin Arc, Western Pacific Ocean. The cells of strain SS33T were motile short rods with a single polar flagellum. The growth of strain SS33T was observed at the temperature range between 33 and 55 °C (optimum growth at 45 °C), at the pH range between 5.0 and 7.1 (optimum growth at pH 6.0) and in the presence of between 2.0 and 4.5% (w/v) NaCl [optimum growth at 3.5% (w/v)]. Strain SS33T was a facultative anaerobic chemolithoautotroph using molecular hydrogen and elemental sulphur as the sole electron donor. Nitrate, nitrous oxide, sulphate, elemental sulphur and molecular oxygen were capable of serving as the sole electron acceptor. Phylogenetic analysis based on 16S rRNA gene sequences placed strain SS33T in the genus Hydrogenimonas belonging to the class Epsilonproteobacteria. The closely related species of strain SS33T were Hydrogenimonas urashimensis SSM-Sur55T (95.96%), Hydrogenimonas thermophila EP1-55-1%T (95.75%) and Hydrogenimonas cancrithermarum ISO32T (95.24%). According to the taxonomic and physiological characteristics, it is proposed that strain SS33T was classified into a novel species of genus Hydrogenimonas, Hydrogenimonas leucolamina sp. nov., with SS33T (=JCM 39184T =KCTC 25253T) as the type strain. Furthermore, the genome comparison of Epsilonproteobacteria revealed that their [NiFe] hydrogenase genes belonging to Group 1b could be divided into two phylogenetic lineages and suggested that the reverse gyrase gene has been lost after division to the genus Hydrogenimonas. - Shogo Yamaki, Koji YamazakiArchives of Virology 169 (9) 182 0304-8608 2024/08/17 [Refereed][Not invited]
- Rika Kudo, Ryota Yamano, Juanwen Yu, Shuya Hatakeyama, Chunqi Jiang, Sayaka Mino, Shogo Yamaki, Yasuhiro Ando, Yuichi Sakai, Tomoo SawabeCurrent Microbiology 81 (8) 246 0343-8651 2024/06/28 [Refereed]
- Sayaka Mino, So Fukazawa, Jiro Tsuchiya, Jesse C. McNichol, Stefan M. Sievert, Shogo Yamaki, Yasuhiro Ando, Tomoo SawabeInternational Journal of Systematic and Evolutionary Microbiology 73 (11) 006132 1466-5026 2023/11/03 [Refereed]
A novel mesophilic, hydrogen- and thiosulfate-oxidizing bacterium, strain ISO32T, was isolated from diffuse-flow hydrothermal fluids from the Crab Spa vent on the East Pacific Rise. Cells of ISO32T were rods, being motile by means of a single polar flagellum. The isolate grew at a temperature range between 30 and 55 °C (optimum, 43 °C), at a pH range between 5.3 and 7.6 (optimum, pH 5.8) and in the presence of 2.0–4.0 % NaCl (optimum, 2.5 %). The isolate was able to grow chemolithoautotrophically with molecular hydrogen, thiosulfate or elemental sulfur as the sole electron donor. Thiosulfate, elemental sulfur, nitrate and molecular oxygen were each used as a sole electron acceptor. Phylogenetic analysis of 16S rRNA gene sequences placed ISO32T in the genus Hydrogenimonas of the class Epsilonproteobacteria, with Hydrogenimonas thermophila EP1-55–1 %T as its closest relative (95.95 % similarity). On the basis of the phylogenetic, physiological and genomic characteristics, it is proposed that the organism represents a novel species within the genus Hydrogenimonas, Hydrogenimonas cancrithermarum sp. nov. The type strain is ISO32T (=JCM 39185T =KCTC 25252T). Furthermore, the genomic properties of members of the genus Hydrogenimonas are distinguished from those of members of other thermophilic genera in the orders Campylobacterales (Nitratiruptor and Nitrosophilus) and Nautiliales (Caminibacter, Nautilia and Lebetimonas), with larger genome sizes and lower 16S rRNA G+C content values. Comprehensive metabolic comparisons based on genomes revealed that genes responsible for the Pta–AckA pathway were observed exclusively in members of mesophilic genera in the order Campylobacterales and of the genus Hydrogenimonas. Our results indicate that the genus Hydrogenimonas contributes to elucidating the evolutionary history of Epsilonproteobacteria in terms of metabolism and transition from a thermophilic to a mesophilic lifestyle. - Di Wang, Chunsheng Li, Chuang Pan, Yueqi Wang, Huan Xiang, Yang Feng, Xianqing Yang, Shengjun Chen, Yongqiang Zhao, Yanyan Wu, Laihao Li, Yuji Kawai, Koji Yamazaki, Shogo YamakiLWT 165 113758 - 113758 0023-6438 2022/08 [Refereed][Not invited]
- Shogo Yamaki, Koji Yamazaki, Yuji KawaiInternational Journal of Food Microbiology 372 109680 - 109680 0168-1605 2022/07 [Refereed][Not invited]
- Shogo Yamaki, Aoi Sakanoue, Kosuke Arai, Koji Yamazaki, Yuji KawaiFood Science and Technology Research 28 (6) 489 - 499 1344-6606 2022 [Refereed][Not invited]
- Tetsuya Kobayashi, Takanori Azuma, Daisuke Yasokawa, Shogo Yamaki, Koji YamazakiBiocontrol Science 26 (3) 147 - 155 1342-4815 2021/09 [Refereed][Not invited]
In this study, spore heat resistance and growth ability at refrigeration temperatures of Bacillus spp. and Paenibacillus spp. were determined. The spore D90°C of 67.6% (23 of 34 strains) of Bacillus and 73.9% (17 of 23 strains) of Paenibacillus was less than 15 min. The growth abilities of both genera were equivalent at 10°C. However, 71.1% (32 of 45 strains) of Paenibacillus and only 6.3% (3 of 48 strains) of Bacillus cereus group could grow at 4°C. Eight B. cereus strains formed spores with higher heat resistance compared to the other Bacillus strains assessed; however, they did not grow at tempreratures below 10°C. Conversely, four Paenibacillus strains formed spores with heat resistance equivalent to that of the eight B. cereus strains and grew at 6°C or lower. In particular, Paenibacillus sp. JCM13343 formed the highest heat-resistant spores (D90°C = 136.1 min) and grew well at 4°C. These results indicate that Paenibacillus can grow in processed foods during refrigerated storage and has the potential to cause spoilage as well as Bacillus. Therefore, Paenibacillus should be considered as one of the targets for microbiological control in refrigerated processed foods. - Shogo Yamaki, Yuji Kawai, Koji YamazakiFood Control 124 107860 0956-7135 2021 [Refereed][Not invited]
- Shogo Yamaki, Koji YamazakiNippon Shokuhin Kagaku Kogaku Kaishi 日本食品科学工学会誌 67 (10) 352 - 359 1341-027X 2020/10/15 [Refereed][Invited]
- Di Wang, Shogo Yamaki, Yuji Kawai, Koji YamazakiLWT 126 109263 - 109263 0023-6438 2020/05 [Refereed][Not invited]
- Shogo Yamaki, Soya Kuronuma, Yuji Kawai, Koji YamazakiInternational Journal of Food Microbiology 317 108457 - 108457 0168-1605 2020/03 [Refereed][Not invited]
- Di Wang, Shogo Yamaki, Yuji Kawai, Koji YamazakiCurrent Microbiology 77 (3) 460 - 467 0343-8651 2020/03 [Refereed][Not invited]
- Shogo Yamaki, Koji YamazakiJapanese Journal of Food Microbiology 日本食品微生物学会 36 (2) 75 - 83 1340-8267 2019/06/30 [Refereed][Invited]
- Shogo Yamaki, Yuji Kawai, Koji YamazakiFood Science and Technology Research 24 (2) 329 - 337 1344-6606 2018 [Refereed][Not invited]
- Kato Riko, Wang Di, Yamaki Shogo, Kawai Yuji, Yamazaki KojiJapanese Journal of Food Microbiology 日本食品微生物学会 34 (3) 158 - 165 1340-8267 2017 [Refereed][Not invited]
Morganella psychrotolerans is a novel psychrotrophic histamine producing bacterium and seems to cause histamine fish poisoning (scombroid poisoning). However, there is little information about the occurrence and histamine producing ability of this bacterium for food contamination. To elucidate the contamination status of M. psychrotolerans, we used an MPN-PCR method that can determine bacterial number more sensitive than a traditional plating method. Many kinds of fish samples purchased in Hokkaido and water samples collected in Hakodate area were used in this study. Eighty-five MPN-PCR positive samples were found in total 192 samples (44.3%) and thirty-seven strains of M. psychrotolerans were isolated from the positive samples. It suggests M. psychrotolerans is widely distributed in retail seafood product in Japan. Then the amounts of histamine produced by M. psychrotolerans isolates were investigated. All isolates produced more than 4,000 mg/L (4,000 ppm) of histamine after 48 h incubation at 25℃. Next, the growth and histamine production of M. psychrotolerans (JCM 16473T, strain Mps. 3 from a horse mackerel sample) and Photobacterium phosphoreum NBRC 103031T was compared in the liquid media containing 0.1 or 2.0% NaCl at 4℃. At 0.1% NaCl, histamine concentration produced by M. psychrotolerans was higher than that by P. phosphoreum. It indicates M. psychrotolerans will also become one of the important bacteria associated with histamine-poisoning at refrigeration temperature on seafood, as well as P. phosphoreum.
- Shogo Yamaki, Naho Hamada, Yuji Kawail, Koji YamazakiJOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI 社団法人 日本食品科学工学会 64 (10) 493 - 501 1341-027X 2017 [Refereed][Not invited]
Bacterial biofilms of foodborne pathogens pose the risk of secondary contamination in a food processing environment. Listeria monocytogenes is a Gram-positive, foodborne pathogen that contaminates various foods and environments. This bacterium can form biofilms on food-related surfaces. In this study, to inhibit biofilm formation by L. monocytogenes, we focused on suppressing the initial adhesion of the pathogen onto polystyrene dishes, which is the first step of biofilm formation. Screening of adhesion inhibitory materials using polystyrene dishes surface treated with food-related materials demonstrated high inhibitory effects of nisin and whey powder against L. monocytogenes adhesion. Moreover, surface treatment with nisin was able to delay biofilm formation of L. monocytogenes and the dish surface showed bactericidal activity. To improve the adhesion inhibitory effects of nisin, we combined nisin and a sucrose fatty acid ester, sucrose palmitate, for surface treatment, and this combination markedly improved the adhesion inhibitory effects. Thus, combined surface treatment with nisin and sucrose palmitate may be an effective method to prevent biofilm formation by L. monocytogenes in food processing environments. - Shogo Yamaki, Shinya Shirahama, Tetsuya Kobayashi, Yuji Kawai, Koji YamazakiFOOD SCIENCE AND TECHNOLOGY RESEARCH 21 (5) 751 - 755 1344-6606 2015/09 [Refereed][Not invited]
Soy-seasoned salmon roe products are commonly eaten in Japan; long-term preservation of these items requires refrigeration. However, salmon roe products are often contaminated with Listeria monocytogenes, which can potentially cause outbreaks of listeriosis. This study focused on developing a method to inhibit the growth of L. monocytogenes in salmon roe products by treating these foodstuffs with a combination of nisin and commercial pectin-hydrolysate, Neupectin L. We determined that treatment with 0.5 mg g(-1) nisin completely inhibited the growth of L. monocytogenes in raw salmon roe. However, treatment with nisin alone did not inhibit L. monocytogenes in soy-seasoned salmon roes. Further work showed that combined treatment with 0.5 mg g(-1) nisin and 0.5% Neupectin L completely inhibited the growth of L. monocytogenes during incubation at 12 degrees C. Therefore, this combination is an alternative way to control L. monocytogenes growth in salmon roe products. - S. Yamaki, Y. Kawai, K. YamazakiJOURNAL OF APPLIED MICROBIOLOGY 118 (6) 1541 - 1550 1364-5072 2015/06 [Refereed][Not invited]
AimsPhotobacterium damselae subsp. damselae is a potent histamine-producing micro-organism. The aim of this study was to isolate and characterize a bacteriophage Phda1 that infected P.damselae subsp. damselae to inhibit its growth and histamine accumulation. Methods and ResultsPhda1 was isolated from a raw oyster, and the host range, morphology and the bacteriophage genome size were analysed. Phda1 formed a clear plaque only against P.damselae subsp. damselae JCM8969 among five Gram-positive and 32 Gram-negative bacterial strains tested. Phda1 belongs to the family Myoviridae, and its genome size was estimated as 352-395kb. According to the one-step growth curve analysis, the latent period, rise period and burst size of Phda1 were 60min, 50min and 19plaque-forming units per infected cell, respectively. Divalent cations, especially Ca2+ and Mg2+, strongly improved Phda1 adsorption to the host cells and its propagation. Phda1 treatment delayed the growth and histamine production of P.damselae subsp. damselae in an invitro challenge test. ConclusionsThe bacteriophage Phda1 might serve as a potential antimicrobial agent to inhibit the histamine poisoning caused by P.damselae subsp. damselae. Significance and Impact of the StudyThis is the first description of a bacteriophage specifically infecting P.damselae subsp. damselae and its potential applications. Bacteriophage therapy could prove useful in the prevention of histamine poisoning. - Shogo Yamaki, Takuo Omachi, Yuji Kawai, Koji YamazakiFEMS MICROBIOLOGY LETTERS 359 (2) 166 - 172 0378-1097 2014/10 [Refereed][Not invited]
Morganella morganii has been identified as a causative agent of opportunistic infections and histamine poisoning. Bacteriophage is a virus and has recently been considered an alternative agent to antibiotics for the control of bacteria that have developed antibiotic resistance. In this study, a novel M.morganii bacteriophage isolated from river water was characterized. The isolated phage, termed FSP1, was purified by polyethylene glycol precipitation followed by cesium chloride density-gradient centrifugation. FSP1 has infectivity against only M.morganii and was identified as a Myoviridae bacteriophage through morphological analysis with transmission electron microscopy. According to the one-step growth curve, the FSP1 latent period, eclipse period, and burst size were 30, 20min, and 42PFU infected cell(-1), respectively. The genome size of FSP1 was estimated to be c. 45.6-49.4kb by restriction endonuclease analyses. Moreover, challenge testing against M.morganii in vitro revealed that FSP1 had high lytic activity and that the viable cell count of M.morganii was reduced by 6.12 log CFUmL(-1) after inoculation with FSP1 at a multiplicity of infection (MOI)=10. These results suggested that FSP1 could be used as a biocontrol agent against M.morganii for treatment of infectious disease treatment or food decontamination.
MISC
- Shogo Yamaki NIPPON SUISAN GAKKAISHI 86- (1) 30 -30 2020/01/15 [Not refereed]
- 水産物における損傷菌-食品加工における損傷菌発生とその問題点山木 将悟, 山﨑 浩司 日本防菌防黴学会誌 47- (11) 467 -472 2019/11 [Not refereed][Invited]
- 山木 将悟, 山崎 浩司 New food industry = ニューフードインダストリー : 食品加工および資材の新知識 59- (7) 15 -23 2017/07 [Not refereed][Invited]
- 山木 将悟, 山﨑 浩司 日本醸造協会誌 109- (9) 636 -642 2014/09 [Not refereed][Invited]
近年，消費者は加熱・加工を極力控えた食品を好むようになっている。このような消費者のニーズに応えつつ食品の安全を担保することは至難の業である。そこで，著者らに食品保存料として世界で広く利用され，食品における食中毒リスクの低減と品質の向上に大きく寄与し，食品の味，香りに影響なく，ヒトの消化酵素により分解される安全な天然由来抗菌ペプチドのバクテリオシンの代表のNisin A，さらにはバクテリオシンと他の抗菌物質との組み合わせなどを解説いただいた。味噌には精製ナイシンとして，5mg/kgの使用が認可されているので，味噌業界の方はご一読いただきたい。
Books etc
- 山木将悟 (Contributorコラム「ヒスタミン食中毒」)
朝倉書店 2023/04 (ISBN: 9784254431308) ix, 147p 100–101 - 食品製造・検査における芽胞・損傷菌とその検出・制御技術山木将悟, 山﨑浩司 (Joint work第II編 損傷菌の検出と制御 第2章5-「リステリア損傷菌の発生と制御」)
シーエムシー出版 2020/05 - Seafood Safety and Quality - Chapter 5Koji Yamazaki, Shogo Yamaki, Dominic Kasujja Bagenda (Joint workChapter 5 - Control of pathogens and spoilage bacteria on seafood products)
CRC Press 2018/09 - Seafood Safety and Quality - Chapter 3Shogo Yamaki, Koji Yamazaki (Joint workChapter 3 - Foodborne pathogens related to seafood products)
CRC Press 2018/09
Association Memberships
- American Society for Microbiology International Association for Food Protection JAPANESE SOCIETY OF FOOD MICROBIOLOGY JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY THE JAPANESE SOCIETY OF FISHERIES SCIENCE
Works
Research Projects
- 日本学術振興会：科学研究費助成事業Date (from‐to) : 2022/04 -2026/03Author : 山木 将悟腸炎ビブリオは水産物を汚染する食中毒細菌である。本菌は海水や水産物に広く分布しており，低温管理から逸脱した水産物の生食などにより食中毒の原因となる。近年，食中毒細菌の制御を目的として細菌に感染するウイルスであるバクテリオファージ（ファージ）を利用した微生物制御手法が注目されている。本研究では，腸炎ビブリオに感染するファージを分離し，その宿主特異性に関して明らかにした上で，腸炎ビブリオファージを利用した腸炎ビブリオの制御手法を構築することを目的として研究を行った。 令和4年度は，腸炎ビブリオファージの分離に取り組み，複数の腸炎ビブリオファージの分離に成功した。分離ファージの中から，様々な腸炎ビブリオの菌株に対してプラークを形成するファージVF-16を選抜し，一段増殖実験や吸着動態の観察，透過型電子顕微鏡によるファージの形態観察などを行なった。腸炎ビブリオファージVF-16は非収縮性の長い尾部を持つ（siphovirus）ファージであり，迅速に宿主細胞へと吸着することが明らかとなった。さらに，VF-16の増殖には，培地へのカルシウムイオンやマグネシウムイオンの添加が必要であり，腸炎ビブリオの増殖を阻害するためにはVF-16と二価カチオンを併用する必要があると推察された。実際に，カルシウムイオンとマグネシウムイオンを添加した液体培地で腸炎ビブリオの増殖抑制能を調べた結果，VF-16添加区ではVF-16未添加区と比べて腸炎ビブリオの増殖が大きく抑制された。 以上のように，令和4年度は腸炎ビブリオに感染するファージを複数分離するとともに，そのうち一株のVF-16を用いて，腸炎ビブリオファージが腸炎ビブリオの増殖抑制剤となり得る可能性を示すことができた。
- Mayekawa Houonkai Foundation：2019年度学術研究助成Date (from‐to) : 2020/01 -2021/12Author : Shogo Yamaki
- The study on control of histamine poisoning of fishery products with peracetic acidThe South Hokkaido Science Promotion Foundation：平成31年度学術研究助成Date (from‐to) : 2019/04 -2020/03Author : Shogo Yamaki, Yuji Kawai, Koji Yamazaki
- Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-upDate (from‐to) : 2018/08 -2020/03Author : Shogo YamakiIn this study, broad-host-range bacteriophage EscoHU1 was characterized and investigated as an antimicrobial agent for the control of foodborne pathogens such as Escherichia coli and Salmonella enterica. EscoHU1 has head and tail structures, and this phage belongs to family Siphoviridae that has a long and flexible tail. In challenge tests using beef and milk, EscoHU1 reduced viable cell counts of E. coli, but did not reduce that of S. Typhimurium. The results from this study suggest that EscoHU1 has potential as the antimicrobial agent for the control of E. coli in contaminated food.
- Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS FellowsDate (from‐to) : 2016/04 -2018/03Author : Shogo Yamakiヒスタミン食中毒は, ヒスタミンを過剰に蓄積した食品を喫食することにより発生しアレルギー様症状を呈する。食品へのヒスタミンの蓄積はヒスタミン生成菌の働きによって起こるため, 本食中毒の防止にはヒスタミン生成菌の制御方法の確立が必要であると考えられる。そこで本研究では, ヒスタミン食中毒の原因となるヒスタミン生成菌の制御法としてバクテリオファージの利用に着目し, バクテリオファージを用いたヒスタミン生成菌制御の方法について研究した。 平成29年度は, 過去に分離したMorganella morganiiに感染するバクテリオファージの混合物を用い, 耐性菌の出現抑制と食品でのヒスタミン生成菌ならびにヒスタミン蓄積抑制効果について研究を行った。バクテリオファージ混合物の耐性菌出現抑制効果については液体培地の系で実験を行った。バクテリオファージ混合物で宿主細菌を殺菌した結果, 混合物を構成する各バクテリオファージを単独で処理した場合と比較して耐性菌の出現率が有意に減少した。 次に, 食品におけるバクテリオファージ混合物の殺菌効果について検証した。ツナフレークにMorganella morganiiを接種しバクテリオファージ混合物を処理した結果, ツナフレーク保存中の生菌数とヒスタミン蓄積量がバクテリオファージ混合物未処理区と比較して大きく減少した。また, 同様の実験を生のマグロ魚肉を用いて行ったところ, ツナフレークでの実験よりも抑制効果は減少したが, バクテリオファージ混合物は魚肉中のMorganella morganiiの発育とヒスタミン蓄積を大きく抑制した。 以上, 平成29年度はMorganella morganiiのバクテリオファージ耐性菌の出現抑制に対する知見を得ることに成功し, バクテリオファージ混合物を用いた処理による魚肉のヒスタミン蓄積抑制効果を示した。