Researcher basic information

• Ph.D. in Agricultural Chemistry, Hokkaido University

• https://researchmap.jp/read0065432?lang=en

• 200901025842820520

• enteroendocrine cells
• gastrointestinal hormone
• 栄養生理学
• 消化管生理学
• Nutritional physiology

• Life Science, Food sciences, 栄養生理学
• Life Science, Physiology

• Bachelor's degree program, School of Agriculture
• Master's degree program, Graduate School of Agriculture
• Doctoral (PhD) degree program, Graduate School of Agriculture

Research activity information

• Oct. 2026, 日本農芸化学会, 2026年度日本農芸化学会東北・北海道支部合同支部大会 学生優秀発表賞
  ケルセチンとその配糖体におけるin vitroにおけるGLP-1分泌とin vivoにおける血糖上昇抑制作用の検討
  近藤 杏南
• Sep. 2026, 日本アミノ酸学会, 日本アミノ酸学会第1９回学術大会優秀発表賞
  アルロースとアミノ酸の共投与による血糖上昇抑制作用の検討
  水間 志織
• Feb. 2026, 日本農芸化学会, 2025年 B.B.B.論文賞
  Oral administration of L-lysine potently promotes glucagon-like peptide-1 secretion and lowers glycemic response in rats
  入江 明歩、比良 徹
• Nov. 2025, 日本アミノ酸学会, 日本アミノ酸学会第1９回学術大会優秀発表賞
  ラットにおいてL-リジンとカルシウムの経口投与は血糖上昇を抑制する
  入江 明歩
• May 2024, 日本栄養・食糧学会, 第78回日本栄養・食糧学会大会 優秀発表賞
  ラットにおいて希少糖アルロースにより生じる回腸の伸展がGLP-1 分泌を促進する
  水間 志織
• May 2024, 日本栄養・食糧学会, 第78回日本栄養・食糧学会 トピックス賞
  「ラットにおいて希少糖アルロースにより生じる回腸の伸展がGLP-1 分泌を促進する」
  水間 志織、比良 徹
• Dec. 2023, 2023年度 日本農芸化学会北海道支部 第二回学術講演会, 学生優秀発表賞
  ラットにおけるL-リジンのGLP-1 分泌促進作⽤
  入江 明歩
• Nov. 2023, 日本食物繊維学会, 日本食物繊維学会第28回学術集会発表賞
  アルロースによる伸展刺激を介したGLP-1分泌機構の解明
  水間 志織
• Nov. 2022, 日本食物繊維学会, 令和4年度 日本食物繊維学会 学会賞
  ルミナコイドによる消化管ホルモン分泌促進作用
  比良 徹
• Jul. 2021, 日本農芸化学会北海道支部, 2021年度日本農芸化学会北海道支部第１回講演会 学生優秀発表賞
  ラットにおけるカルシウムによる食欲抑制に対する食事組成の影響
  原田 和馬, Japan society
• May 2021, 日本栄養·食糧学会, 令和3年度 日本栄養·食糧学会トピックス賞
  2-モノアシルグリセロールのCCK分泌活性評価およびその作用機構の解明
  落合 啓太、廣岡 里菜、境野 眞善、竹内 茂雄、比良 徹, Japan society
• Dec. 2020, 日本栄養・食糧学会北海道支部, 2020年度 日本農芸化学会北海道支部／第50回 日本栄養・食糧学会北海道支部 合同学術講演会 若手奨励賞
  ラットにおける伸展刺激負荷による GLP-1 分泌応答の解析
  木村 夏織, Japan society
• Mar. 2020, 日本農芸化学会, 日本農芸化学会 2020年度大会 優秀発表賞
  食事誘導性肥満ラットにおけるGLP-1分泌応答の増強には回腸部が寄与する
  佐野 杏莉, Japan society
• Nov. 2019, 日本食物繊維学会, 日本食物繊維学会第 24回学術集会 発表賞
  ラットにおける D- アルロースによる GLP-1 分泌促進機構の解析
  木村夏織, Japan society
• Nov. 2019, 日本農芸化学会北海道支部, 日本農芸化学会 北海道支部第２回講演会 学生優秀発表賞
  食事誘導性肥満ラットにおけるGLP-1の分泌応答と小腸上部および下部での違い
  佐野 杏莉, Japan society
• Sep. 2019, 日本栄養・食糧学会北海道支部, 第49回 日本栄養・食糧学会北海道支部会 若手奨励賞
  タンパク質投与で生じる高い GLP-1 応答への DPP-4 の関与
  清水 祐希, Japan society
• Jul. 2019, 日本農芸化学会北海道支部, 2019年度日本農芸化学会 北海道支部第１回講演会 学生優秀発表賞
  ラットへの食用油経口投与による胃排出への影響
  李 佳昕, Japan society
• May 2018, 日本栄養・食糧学会, 第 72 回日本栄養・食糧学会大会 トピックス演題
  Zein加水分解物による小腸部位別でのGLP-1分泌応答
  清水 祐希;比良 徹;原 博, Japan society
• Nov. 2017, 第24回日本未病システム学会学術総会 優秀演題賞
  ケルセチン配糖体の反復投与は糖負荷後のラットGLP-1分泌能を増強する
  長瀬 僚介, Japan society
• Jul. 2017, 日本農芸化学会北海道支部, 平成29年度日本農芸化学会北海道支部第１回講演会学生優秀発表賞
  The role of endogenous GLP-1 against the development of glucose intolerance in rats fed a high fat and high sucrose diet
  Jukkrapong Pinyo, Japan society
• May 2017, 日本栄養・食糧学会, 第 71 回日本栄養・食糧学会大会 学生優秀発表賞
  肥満誘導食摂取ラットにおける食事刺激に対するGLP-1分泌応答の解析
  柳原 くるみ, Japan society
• Nov. 2016, 日本食物繊維学会, 日本食物繊維学会 第21回学術集会 発表賞
  D-プシコースによる GLP-1 分泌促進作用における糖輸送担体の関与
  早川 真輝, Japan society
• May 2016, 日本栄養・食糧学会, 第70回日本栄養・食糧学会大会 学生優秀発表賞
  D-プシコースの経口投与はラットにおいてGLP-1分泌を促進する
  早川 真輝, Japan society
• Sep. 2014, The XXVIIth International Conference on Polyphenols (ICP 2014) and 8th Tannin Conference, The XXVIIth International Conference on Polyphenols (ICP 2014) and 8th Tannin Conference Poster Award
  A non-digestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid, α-glucosyl-isoquercetin, on glucagon-like peptide 1 (GLP-1) secretion in rat intestine and enteroendocrine cells.
  Phuwamongkolwiwat P, International society
• Nov. 2013, 日本アミノ酸学会, 2013年度日本アミノ酸学会 科学・技術賞
  比良 徹, Japan society
• Nov. 2012, 日本食物繊維学会 17回学術集会, 日本食物繊維学会 17回学術集会 発表賞
  難消化性デキストリンの単回及び長期摂取はラットのGLP-1分泌を促進する
  池江 明日香, Japan society
• Sep. 2012, The 25th Anniversary of the Discovery of GLP-1 symposium, Young Investigator Abstract Competition Honorable Mention
  HIRA Tohru, International society
• May 2012, 日本栄養・食糧学会, 平成24年度日本栄養・食糧学会奨励賞
  比良 徹, Japan society
• Nov. 2011, 2011 The International Conference on Food Factors, 2011 The International Conference on Food Factors, Travel Award
  Fructooligosaccharides enhances beneficial effects of a flavonoid, quercetin-3-β-glucoside, on insulin sensitivity in rats.
  Phuwamongkolwiwat P, International society
• Nov. 2011, 日本食物繊維学会 16 回学術集会, 日本食物繊維学会 16 回学術集会 発表賞
  トウモロコシたんぱく質 Zein の加水分解物による血糖上昇抑制への GLP-1 の関与
  樋口 謹行
• Sep. 2010, The 18th International Symposium on Regulatory Peptides, The 18th International Symposium on Regulatory Peptides, Poster Award
  Calcium-sensing receptor is a sensor for dietary peptides to stimulate cholecystokinin secretion in the enteroendocrine cell line STC-1
  S. Nakajima
• Sep. 2010, 日本アミノ酸学会, 日本アミノ酸学会第4回学術大会 ポスター賞
  ラットにおけるCalcium-sensing receptorアゴニストによる血糖上昇抑制作用とその作用機構
  村松 茉耶
• Nov. 2008, 日本食物繊維学会, 第13回日本食物繊維学会学術集会 奨励賞
  トウモロコシの難消化性たんぱく質Zeinの加水分解物によるラットにおける Glucagon-like peptide-1分泌機構
  持田 泰佑

• Improved measurement of GLP-1 concentrations in mouse plasma by ex vivo inhibition of neprilysin.
  Chikayuki Funabashi; Tohru Hira
  Biochemical and biophysical research communications, 839, 154721, 154721, 09 Oct. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Accurate measurement of plasma glucagon-like peptide-1 (GLP-1) in mice is hindered by its rapid degradation by dipeptidyl peptidase-4 (DPP-4) and neprilysin (NEP). In vivo administration of these inhibitors can stabilize plasma GLP-1. However, GLP-1-mediated physiological functions, such as enhancing insulin secretion and suppressing gastric emptying, can be artificially altered by these inhibitors. Here, we aimed to establish a simple and reliable ex vivo inhibition method by adding the NEP inhibitor, sacubitrilat, to collection tubes. The efficacy of sacubitrilat was validated through an in vitro GLP-1 degradation assay. The ex vivo method was then evaluated during meal and glucose tolerance tests for measurement of plasma GLP-1, and compared with an in vivo inhibition method, with plasma glucose, insulin, and gastric emptying measured in parallel. In the in vitro assay, sacubitrilat addition effectively prevented GLP-1 degradation. In meal and oral glucose tolerance tests, ex vivo sacubitrilat addition yielded markedly higher plasma GLP-1 concentrations compared to standard inhibitor cocktails containing DPP-4 inhibitor, enabling clear detection of nutrient-induced GLP-1 peak. This ex vivo approach did not influence plasma glucose, insulin, and gastric emptying, thus preserving the physiological integrity of the metabolic response. In contrast to the ex vivo inhibition method, the in vivo inhibition method yielded higher GLP-1 concentrations accompanied by lowered glucose, enhanced insulin, and reduced gastric emptying. Our findings demonstrate that the addition of the NEP inhibitor to tubes is an effective strategy for the precise measurement of GLP-1 secretory dynamics, particularly in the measurement of multiple physiological parameters.
• 抗肥満・抗糖尿病ホルモンGLP-1の新たな分泌メカニズム 腸の伸展を介したGLP-1分泌
  水間 志織; 比良 徹
  化学と生物, 64, 3, 127, 128, (公社)日本農芸化学会, May 2026, [Peer-reviewed], [Last author, Corresponding author]
  Japanese
• Oral administration of l-lysine potently promotes GLP-1 secretion and lowers glycemic response in rats.
  Akiho Irie; Tohru Hira
  Bioscience, biotechnology, and biochemistry, 03 Sep. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Glucagon-like peptide-1 (GLP-1) is a gastrointestinal hormone that suppresses postprandial glycemia and appetite. GLP-1 secretion is promoted by various nutrients; however, studies on the effects of orally administered amino acids on GLP-1 secretion are limited. This study investigated the acute effects of l-lysine (Lys) on GLP-1 secretion in vivo and explored the underlying mechanisms. In male Sprague-Dawley rats, oral administration Lys, but not l-Alanine (Ala), elevated plasma GLP-1 concentrations, with comparable to or higher potency compared to other amino acids known to stimulate GLP-1 secretion. Duodenal administration of CaSR antagonist attenuated Lys-induced GLP-1 secretion. Oral co-administration of Lys and calcium potently promoted GLP-1 secretion and lowered glycemia under an oral glucose tolerance test. These findings demonstrate that Lys potently induces GLP-1 secretion in vivo, and the combination of Lys and calcium has translational potential as potent GLP-1 releasing strategy for the management of blood glucose levels.
• Glucagon-like peptide-1 responses to carbohydrate, fat and protein in rats during the development of diet-induced obesity and the role of the distal small intestine.
  Anri Sano; Satomi Tanaka; Kurumi Yanagihara; Yasuko Sakihama; Tohru Hira
  The British journal of nutrition, 1, 9, 28 Jul. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, The secretion of glucagon-like peptide-1 (GLP-1) is stimulated by luminal nutrients after meal ingestion. Diet-induced obesity (DIO) may affect nutrient-induced GLP-1 secretion in humans and rodent models. We previously demonstrated that mixed meal-induced GLP-1 secretion is enhanced in rats with DIO compared with normal rats. However, it is unclear to which nutrient the GLP-1 secretion is adaptively enhanced or reduced during the development of DIO. The present study investigated the effect of obesity on the GLP-1 secretion to individual nutrients and further on GLP-1 secretory functions of the proximal and distal small intestine in rats. Male Sprague-Dawley rats were fed a control diet or a high-fat diet with sucrose solution (HFS) for 4–5 weeks. GLP-1 responses to a single oral administration of a liquid diet, dextrin, soyabean oil or whey protein were examined after 4 weeks of dietary intervention. In addition, a liquid diet was administered to the proximal or distal small intestine of anaesthetised rats (control or HFS), and GLP-1 levels in the portal vein plasma were measured. In HFS-fed rats, GLP-1 secretion to dextrin, soyabean oil and whey protein slightly increased compared with those in normal rats. Furthermore, the GLP-1 response to liquid diet administration into the lumen was greater in the distal, but not proximal, small intestine of HFS-fed rats than that in control rats. In rats with DIO, GLP-1 secretion increased, regardless of the type of nutrient. Furthermore, the distal small intestine is responsible for adaptive enhancement of the GLP-1 secretion.
• Abilities of Rare Sugar Members to Release Glucagon-like Peptide-1 and Suppress Food Intake in Mice.
  Yuta Masuda; Kento Ohbayashi; Kengo Iba; Rika Kitano; Tomonori Kimura; Takako Yamada; Tohru Hira; Toshihiko Yada; Yusaku Iwasaki
  Nutrients, 17, 7, 31 Mar. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Background/Objectives: Rare sugars, which naturally exist in small quantities, have gained attention as next-generation functional sugars due to their sweetness and low calorie content. Some of them have already been commercialized. Rare sugar-containing syrups, produced through alkaline isomerization of high-fructose corn syrup, are effective in preventing obesity and type 2 diabetes. However, the mechanisms underlying these effects remain incompletely understood. Recently, D-allulose has been found to improve hyperphagic obesity by stimulating the secretion of the intestinal hormone glucagon-like peptide-1 (GLP-1). The present study aimed to determine the comparative effects of aldohexoses (D-glucose, D-allose) and ketohexoses (D-fructose, D-allulose, D-tagatose, D-sorbose) on GLP-1 secretion and food intake in male mice. Method and Results: Single peroral administration of four ketohexoses at 1 and 3 g/kg, but not aldohexoses at 1 and 3 g/kg, significantly increased plasma GLP-1 concentrations with comparable efficacy. Moreover, these ketohexoses at 1 g/kg suppressed food intake in the short term, an effect blunted by GLP-1 receptor antagonism. In contrast, zero-calorie D-allose at 3 g/kg suppressed feeding without raising plasma GLP-1 levels. Conclusions: These results demonstrate that D-allulose, D-tagatose, and D-sorbose, which are low-calorie rare sugars classified as ketohexoses, suppress food intake through promoting GLP-1 secretion, showing their potential to prevent and/or ameliorate type 2 diabetes, obesity and related diseases.
• Intestinal Distension Induced by Luminal D-allulose Promotes GLP-1 Secretion in Male Rats.
  Shiori Mizuma; Masaki Hayakawa; Tohru Hira
  Endocrinology, 166, 2, 06 Jan. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, The secretion of glucagon-like peptide-1 (GLP-1) is promoted by various nutrients, and glucose and fructose stimulate GLP-1 secretion via intracellular metabolism. D-allulose (allulose), a nonmetabolizable epimer of D-fructose, is also effective in stimulating GLP-1 secretion, although its underlying mechanism remains unclear. We previously observed intestinal distension after the oral administration of allulose, accompanied by increased GLP-1 secretion in rats, possibly because of the low or slow absorbability of allulose. In this study, we sought to determine whether intestinal distension caused by allulose and other factors gives rise to GLP-1 secretion in rats. We found that the oral coadministration of carbonated water enhanced allulose-induced GLP-1 secretion. Polyethylene glycol 1000 and D-mannitol, which are water-soluble and poorly absorbable, stimulated GLP-1 secretion. However, cellulose (insoluble), and tetra ethylene glycol (water-soluble and absorbable) did not. The secretion of GLP-1 increased as the absolute amount of allulose increased, independent of the concentration. The extent of the GLP-1 secretory response was positively correlated with the intestinal content volume and diameter after allulose administration. Furthermore, the intraileal administration of air expanded the intestine-induced secretion of GLP-1. Our results demonstrate that allulose promotes GLP-1 secretion, at least in part, via intestinal distension as a novel GLP-1 secretory mechanism. Physical stimulation may also contribute to postprandial GLP-1 secretion.
• Differential Regulation of Cholecystokinin Production by Synthetic and Natural Glucocorticoids in Enteroendocrine Cells.
  Shingo Nakajima; Shoko Tsuchimine; Tohru Hira; Hiroshi Kunugi
  Journal of nutritional science and vitaminology, 71, 5, 411, 420, 2025, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Chronic stress often causes abnormal eating behavior and metabolism due at least in part to the activation of hypothalamic-pituitary-adrenal (HPA) axis resulting in increases glucocorticoids secretion. Cholecystokinin (CCK) is an anorexic gut hormone secreted from enteroendocrine I cells and controls digestion through the gastric emptying and digestive enzyme secretion. In the present study, we highlighted the function of glucocorticoids in CCK-producing cells with enteroendocrine model cell lines and male Wistar rats. We found that glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) are expressed in STC-1 cells known as an I cell model line. Synthetic (dexamethasone; DEX) and endogenous (corticosterone; CORT) glucocorticoids reduced CCK transcription in STC-1 cells. Depolarization-induced CCK secretion was attenuated by both glucocorticoids, whereas dietary peptide-induced CCK secretion was enhanced by acute CORT treatment. DEX and CORT treatments weakened phosphorylated signaling pathway for CCK transcription in an agonist-specific manner. Daily single injection of DEX but not CORT for 4 d decreased Cck mRNA in the upper small intestine with altered metabolism and gut glucocorticoid sensitivity in male Wistar rats. These findings provide a new insight to reveal stress-induced abnormal nutrient and metabolic status in relation to gastrointestinal dysfunctions.
• Amino acid transport dynamics in the jejunum and ileum in rats: a regional and time-course analysis.
  Kojiro Katsura; Tohru Hira
  The British journal of nutrition, 132, 10, 1278, 1288, 28 Nov. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Postprandially, amino acids and di/tripeptides are thought to be primarily absorbed in the proximal small intestine. However, there have been no in vivo demonstrations of regional differences in amino acid transport dynamics between the proximal and distal small intestines. We monitored plasma amino acid responses in the jejunal and ileal mesenteric veins of rats after oral administration of a diet or an amino acid mixture (Expt 1) and in the portal vein after direct administration of the amino acid mixture into the lumen of the jejunum or ileum (Expt 2). In Expt 1, the total and some amino acid concentrations in the jejunal mesenteric vein were slightly higher than those in the ileal mesenteric vein after oral administration of the amino acid mixture, suggesting that the ileum actively transports luminal amino acids to the basolateral side, comparable to the jejunum. In Expt 2, portal amino acid concentrations were elevated to a greater extent after direct administration of the amino acid mixture into the ileal lumen than into the jejunal lumen. These results demonstrate regional differences in amino acid transport dynamics in vivo and suggest that the ileum has a higher capacity for transporting amino acids than the jejunum. Our findings highlight the importance of the ileum in postprandial amino acid absorption and metabolism.
• Targeting intestinal neprilysin to ameliorate glucose homeostasis through insulinotropic effect.
  Shiori Mizuma; Tohru Hira
  Endocrinology, 27 Sep. 2024, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
  English
• Effect of Single Oral Coingestion of GABA and Malic Acid on Postprandial GLP-1, Glucose, and Insulin Responses in Healthy Volunteers: A Randomized, Double-Blind, Placebo-Controlled, Crossover Study.
  Hiroki Noguchi; Takeshi Ikenaga; Shota Ueno; Chieko Kohashi; Yasuhiro Matsumura; Yusuke Kakumoto; Noriyuki Kohda; Hiroshi Hara; Tohru Hira
  Molecular nutrition & food research, 68, 7, e2300610, Apr. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, SCOPE: This study examines whether coingestion of γ-aminobutyric acid (GABA) and malic acid (MA) before meals enhances glucagon-like peptide-1 (GLP-1) secretion, and which affects subsequent insulin and glycemic responses in humans. METHODS AND RESULTS: Initially, a murine enteroendocrine STC-1 cell line is used to verify coadministration of GABA and MA synergistically induces GLP-1 secretion. Next, 22 healthy adults are given water (50 mL) containing 400 mg GABA and 400 mg MA (Test), or only 400 mg citric acid (CA) (Placebo) 20 min before meal tolerance test (MTT). Interval blood samples are taken postprandially over 180 min to determine GLP-1, insulin, and glucose responses. By comparison to preload of Placebo, preload of Test significantly increases plasma GLP-1 (total/active) levels (incremental area under the curve by 1.2- and 1.6-fold), respectively. However, there are no significant differences in postprandial blood glucose and insulin. CONCLUSION: Coingestion of GABA and MA before meals enhances postprandial GLP-1 secretion. Future studies should explore optimal dosage regimens to find the efficacy of the mixture on insulin and glycemic response.
• 食品タンパク質・ペプチドによるGLP-1分泌促進とそれによる血糖上昇抑制、体温上昇作用
  比良 徹
  日本栄養・食糧学会誌, 77, 1, Feb. 2024, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
• Tunable structure of chimeric isomaltomegalosaccharides with double α-(1 → 4)-glucosyl chains enhances the solubility of water-insoluble bioactive compounds
  Weeranuch Lang; Takayoshi Tagami; Yuya Kumagai; Seiya Tanaka; Hye-Jin Kang; Masayuki Okuyama; Wataru Saburi; Haruhide Mori; Tohru Hira; Chaehun Lee; Takuya Isono; Toshifumi Satoh; Hiroshi Hara; Takayuki Kurokawa; Nobuo Sakairi; Yoshiaki Yuguchi; Atsuo Kimura
  Carbohydrate Polymers, 319, 121185, 121185, Elsevier BV, Nov. 2023, [Peer-reviewed]
  Scientific journal
• Synergistic enhancement of glucagon-like peptide-1 release by γ-aminobutyric acid and L-phenylalanine in enteroendocrine cells - searching active ingredients in a water extract of Corn Zein protein.
  Hiroki Noguchi; Noriyuki Kohda; Hiroshi Hara; Tohru Hira
  Bioscience, biotechnology, and biochemistry, 04 Sep. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, This study investigated the glucagon-like peptide-1 (GLP-1)-releasing activity of an aqueous extract (ZeinS) from corn zein protein, and aimed to identify the active compounds responsible for this activity. GLP-1-releasing activity was evaluated using a murine enteroendocrine cell line (GLUTag). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed on purified fractions of ZeinS to identify active molecules. ZeinS stimulated more GLP-1 secretion from GLUTag cells compared to zein hydrolysate. Fractions displaying biological activity were determined by solid phase extraction and high-performance liquid chromatography (HPLC) fractionation. Subsequent LC-MS/MS analysis identified several amino acids in the active fractions of ZeinS. In particular, γ-aminobutyric acid (GABA) exhibited significant GLP-1 releasing activity both alone and synergistically with L-phenylalanine (Phe). Moreover, ZeinS-induced GLP-1 secretion was attenuated by antagonists for the GABA receptor and calcium sensing receptor. These results demonstrate that GABA and Phe identified in ZeinS synergistically stimulate GLP-1 secretion in enteroendocrine cells.
• Trp-Tyr is a dipeptide structure that potently stimulates GLP-1 secretion in a murine enteroendocrine cell model, identified by comprehensive analysis.
  Hayate Taguchi; Kana Murai; Tohru Hira
  Biochemical and biophysical research communications, 661, 28, 33, 18 Jun. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Dietary peptides potently stimulate glucagon-like peptide-1 (GLP-1) secretion, however, the underlying molecular mechanisms, such as structure-activity relationships and sensing mechanisms are only partly elucidated. In this study, we used a dipeptide library to identify dipeptides that potently stimulate GLP-1 release and to clarify the underlying structure-activity relationship. Murine enteroendocrine GLUTag cells were exposed to 339 dipeptides for 60 min, and the concentration of GLP-1 released into the supernatant was measured. Subsequently, selected dipeptides were examined for their reproducibility and dose responsiveness. In addition, we investigated the role of constituent amino acids in the secretion of GLP-1, and whether tripeptides containing the active dipeptide structures maintained their activity. In a concentration range of 1-5 mg/mL, twelve dipeptides had reproducible and concentration-dependent GLP-1-releasing activity. Among them, nine dipeptides (FY, KF, NI, PM, QL, QY, WF, WN, WY) were novel, with WY exhibiting the most potent activity. The reverse sequences and most free amino acids did not induce GLP-1 secretion, indicating that GLP-1-producing cells recognize the structure of each peptide to induce GLP-1 secretion. However, no apparent similarities were found between the active peptides. A comparison between the six tripeptides composed of F, W, and Y revealed the further potent tripeptides FWY and WYF, than WY. In the present study, a comprehensive analysis revealed nine novel dipeptides with high potential to stimulate GLP-1 secretion. Furthermore, the results indicate that 'WY' is a specific dipeptide sequence that potently stimulates GLP-1 secretion.
• Glucagon-like peptide-1 is involved in the thermic effects of dietary proteins in male rodents.
  Keita Ochiai; Asuka Muto; Bong Soo Seok; Yuta Doi; Yusaku Iwasaki; Yuko Okamatsu-Ogura; Daniel J Drucker; Tohru Hira
  Endocrinology, 04 May 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Protein intake potently increases body temperature and energy expenditure, but the underlying mechanism thereof remains incompletely understood. Simultaneously, protein intake potently stimulates glucagon-like peptide-1 (GLP-1) secretion. Here, we examined the involvement of GLP-1 in the thermic effects of dietary proteins in rodents by measuring rectal temperature and energy expenditure and modulating GLP-1 signaling. Rectal temperature of rats or mice fasted for 4 or 5 h were measured using a thermocouple thermometer before and after an oral administration of nutrients. Oxygen consumption after oral protein administration was also measured in rats. Rectal temperature measurements in rats confirmed an increase in core body temperature after refeeding, and the thermic effect of the oral administration of protein was greater than that of a representative carbohydrate or lipid. Among the five dietary proteins examined (casein, whey, rice, egg, and soy), soy protein had the highest thermic effect. The thermic effect of soy protein was also demonstrated by increased oxygen consumption. Studies using a nonselective β-adrenergic receptor antagonist and thermal camera suggested that brown adipose tissue did not contribute to soy protein-induced increase in rectal temperature. Furthermore, the thermic effect of soy protein was completely abolished by antagonism and knockout of GLP-1 receptor, yet potentiated via augmentation of intact GLP-1 levels through inhibition of dipeptidyl peptidase-4 activity. These results indicate that GLP-1 signaling is essential for the thermic effects of dietary proteins in rats and mice, and extend the metabolic actions of GLP-1 ensuing from nutrient ingestion to encompass the thermic response to ingested protein.
• Formulation and evaluation of a novel megalomeric microemulsion from tamarind seed xyloglucan-megalosaccharides for improved high-dose quercetin delivery
  Weeranuch Lang; Debashish Mondol; Aphichat Trakooncharoenvit; Takayoshi Tagami; Masayuki Okuyama; Tohru Hira; Nobuo Sakairi; Atsuo Kimura
  Food Hydrocolloids, 137, Apr. 2023, [Peer-reviewed]
  Scientific journal
• Research on Luminacoids-Indeced Gastrointestinal Hormone Secretion
  比良徹
  ルミナコイド研究, 27, 1, 1, 7, 2023, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
• Direct visualization of glucagon-like peptide-1 secretion by fluorescent fusion proteins.
  Atsushi Tsuzuki; Yoichiro Fujioka; Aiko Yoshida; Sayaka Kashiwagi; Maho Amano; Tohru Hira; Akinobu Nakamura; Hideaki Miyoshi; Tatsuya Atsumi; Yusuke Ohba
  Journal of diabetes investigation, 13, 7, 1134, 1139, 04 Apr. 2022, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Live-cell imaging with fluorescent proteins (FPs) is a powerful tool for investigating the exocytosis processes of hormones. However, the secretion process of glucagon-like peptide-1 (GLP-1) has not been visualized by FPs, which might be because tagging FPs inhibits GLP-1 synthesis through the post-translational processing from proglucagon. Here, we have developed FP-tagged GLP-1 by inserting FPs into the middle of GLP-1 and adding the proglucagon signal peptide. Confocal imaging confirmed that GLP-1 fused to FPs with high folding efficiency showed granular structure, in which secretory vesicle markers colocalized. The fluorescence intensity of FP in the culture supernatant from cells treated with KCl or forskolin was significantly increased compared with those from untreated cells. Furthermore, FP-tagged GLP-1 enables direct visualization of stimulation-dependent exocytosis of GLP-1 at a single granule resolution with total internal reflection fluorescence microscopy. FP-tagged GLP-1 might facilitate the screening of GLP-1 secretagogues and the discovery of new antidiabetic drugs.
• 2-Arachidonoyl glycerol suppresses gastric emptying via the cannabinoid receptor 1-cholecystokinin signaling pathway in mice.
  Keita Ochiai; Rina Hirooka; Masayoshi Sakaino; Shigeo Takeuchi; Tohru Hira
  Lipids, 10 Mar. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, 2-Monoacylglycerol (2-MAG) is one of the digestion products of dietary lipids. We recently demonstrated that a 2-MAG, 2-arachidonoyl glycerol (2-AG) potently stimulated cholecystokinin (CCK) secretion via cannabinoid receptor 1 (CB1) in a murine CCK-producing cell line, STC-1. CCK plays a crucial role in suppressing postprandial gastric emptying. To examine the effect of 2-AG on gastric emptying, we performed acetaminophen and phenol red recovery tests under oral or intraperitoneal administration of 2-AG in mice. Orally administered 2-AG (25 mg/kg) suppressed the gastric emptying rate in mice, as determined by the acetaminophen absorption test and phenol red recovery test. Intraperitoneal administration of a cholecystokinin A receptor antagonist (0.5 mg/kg) attenuated the gastric inhibitory emptying effect. In addition, both oral (10 mg/kg) and intraperitoneal (0.5 mg/kg) administration of a CB1 antagonist counteracted the 2-AG-induced gastric inhibitory effect. Furthermore, intraperitoneal 2-AG (25 mg/kg) suppressed gastric emptying. These results indicate that 2-AG exhibits an inhibitory effect on gastric emptying in mice, possibly mediated by stimulating both CCK secretion via CB1 expressed in CCK-producing cells and acting on CB1 expressed in the peripheral nerves. Our findings provide novel insights into the 2-MAG-sensing mechanism in enteroendocrine cells and the physiological role of 2-MAG.
• Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin.
  Naoki Harada; Mai Okuyama; Yoshiaki Teraoka; Yumi Arahori; Yoh Shinmori; Hiroko Horiuchi; Paula B Luis; Akil I Joseph; Tomoya Kitakaze; Shigenobu Matsumura; Tohru Hira; Norio Yamamoto; Takashi Iuni; Naoki Goshima; Claus Schneider; Hiroshi Inui; Ryoichi Yamaji
  NPJ science of food, 6, 1, 4, 4, 14 Jan. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The identification of molecular targets of bioactive food components is important to understand the mechanistic aspect of their physiological functions. Here, we have developed a screening system that enables us to determine the activation of G protein-coupled receptors (GPCRs) by food components and have identified GPR55 as a target for curcumin. Curcumin activated GPR55 and induced serum-response element- and serum-response factor-mediated transcription, which were inhibited by Rho kinase and GPR55 antagonists. Both the methoxy group and the heptadienone moiety of curcumin were required for GPR55 activation. The F1905.47 residue of GPR55 was important for the interaction with curcumin. The curcumin-induced secretion of glucagon-like peptide-1 in GLUTag cells was inhibited by a GPR55 antagonist. These results indicate that expression screening is a useful system to identify GPCRs as targets of food components and strongly suggest that curcumin activates GPR55 as an agonist, which is involved in the physiological function of curcumin.
• Enteroendocrine Systems That Sense Nutrients in the Gut and Control the Body
  Tohru Hira
  Journal of Nutritional Science and Vitaminology, 68, S5, S7, 2022, [Invited], [Lead author, Last author, Corresponding author]
  Scientific journal
• GLP-1 Responses to Oral Administration of a Dietary Peptide in Rats Fed Resistant Maltodextrin or Fructooligosaccharides
  比良徹; 池江明日香; 岸本由香; 原博
  ルミナコイド研究, 26, 1, 1, 8, 2022, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• 2‐Arachidonoyl glycerol potently induces cholecystokinin secretion in murine enteroendocrine STC ‐1 cells via cannabinoid receptor CB1
  Keita Ochiai; Rina Hirooka; Masayoshi Sakaino; Shigeo Takeuchi; Tohru Hira
  Lipids, Wiley, 17 Sep. 2021, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• Diet-induced obesity enhances postprandial glucagon-like peptide-1 secretion in Wistar rats, but not in diabetic Goto-Kakizaki rats.
  Jukkrapong Pinyo; Hiroshi Hara; Tohru Hira
  The British journal of nutrition, 126, 4, 518, 530, 28 Aug. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Glucagon-like peptide-1 (GLP-1) is postprandially secreted from enteroendocrine L-cells and enhances insulin secretion. Currently, it is still controversial whether postprandial GLP-1 responses are altered in obesity and diabetes. To address the issue and to find out possible factors related, we compared postprandial GLP-1 responses in normal rats and in diabetic rats chronically fed an obesogenic diet. Male Wistar rats and diabetic Goto-Kakizaki (GK) rats were fed either a control diet or a high-fat/high-sucrose (HFS, 30 % fat and 40 % sucrose) diet for 26 weeks. Meal tolerance tests were performed for monitoring postprandial responses after a liquid diet administration (62·76 kJ/kg body weight) every 4 or 8 weeks. Postprandial glucose, GLP-1 and insulin responses in Wistar rats fed the HFS diet (WH) were higher than Wistar rats fed the control diet (WC). Although GK rats fed the HFS diet (GH) had higher glycaemic responses than GK rats fed the control diet (GC), these groups had similar postprandial GLP-1 and insulin responses throughout the study. Jejunal and ileal GLP-1 contents were increased by the HFS diet only in Wistar rats. Furthermore, mRNA expression levels of fatty acid receptors (Ffar1) in the jejunum were mildly (P = 0·053) increased by the HFS diet in Wistar rats, but not in GK rats. These results demonstrate that postprandial GLP-1 responses are enhanced under an obesogenic status in normal rats, but not in diabetic rats. Failure of adaptive enhancement of GLP-1 response in GK rats could be partly responsible for the development of glucose intolerance., 32209234;32209238
• Improvement of Glucose Tolerance by Food Factors Having Glucagon-Like Peptide-1 Releasing Activity.
  Tohru Hira; Aphichat Trakooncharoenvit; Hayate Taguchi; Hiroshi Hara
  International journal of molecular sciences, 22, 12, 21 Jun. 2021, [Peer-reviewed], [Invited], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, Glucagon-like peptide-1 (GLP-1) is a gastrointestinal hormone released from enteroendocrine L cells in response to meal ingestion. GLP-1 receptor agonists and GLP-1 enhancers have been clinically employed to treat diabetes owing to their glucose-dependent insulin-releasing activity. The release of GLP-1 is primarily stimulated by macronutrients such as glucose and fatty acids, which are nutritionally indispensable; however, excessive intake of sugar and fat is responsible for the development of obesity and diabetes. Therefore, GLP-1 releasing food factors, such as dietary peptides and non-nutrients, are deemed desirable for improving glucose tolerance. Human and animal studies have revealed that dietary proteins/peptides have a potent effect on stimulating GLP-1 secretion. Studies in enteroendocrine cell models have shown that dietary peptides, amino acids, and phytochemicals, such as quercetin, can directly stimulate GLP-1 secretion. In our animal experiments, these food factors improved glucose metabolism and increased GLP-1 secretion. Furthermore, some dietary peptides not only stimulated GLP-1 secretion but also reduced plasma peptidase activity, which is responsible for GLP-1 inactivation. Herein, we review the relationship between GLP-1 and food factors, especially dietary peptides and flavonoids. Accordingly, utilization of food factors with GLP-1-releasing/enhancing activity is a promising strategy for preventing and treating obesity and diabetes.
• Combination of α-Glycosyl-Isoquercitrin and Soybean Fiber Promotes Quercetin Bioavailability and Glucagon-like Peptide-1 Secretion and Improves Glucose Homeostasis in Rats Fed a High-Fat High-Sucrose Diet.
  Aphichat Trakooncharoenvit; Hiroshi Hara; Tohru Hira
  Journal of agricultural and food chemistry, 69, 21, 5907, 5916, 02 Jun. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, This study examined the effects of a combination of soybean fiber and α-glycosyl-isoquercitrin (AGIQ) on improving quercetin bioavailability and glucose metabolism in rats fed an obesogenic diet. For 9 weeks, rats were individually fed a control diet, a high-fat high-sucrose (H) diet, H with soybean fiber (HS), or with AGIQ (HQ), or with both (HSQ). Quercetin derivatives in plasma, feces, urine, and cecal content were quantified by high-performance liquid chromatography to assess the bioavailability of quercetin, and meal tolerance tests were performed to assess postprandial glycemia and glucagon-like peptide-1 (GLP-1) responses. The HSQ group had higher plasma quercetin levels than HQ. The postprandial glycemia was attenuated in the HSQ group when compared to the H group. The basal plasma GLP-1 concentrations positively correlated with plasma quercetin derivative concentrations. Hence, the combination of soybean fiber and AGIQ could be beneficial for reducing the risk of glucose intolerance, possibly involving enhanced quercetin bioavailability and GLP-1 secretion., 32209234;32209236
• Acute Oral Calcium Suppresses Food Intake Through Enhanced Peptide-YY Secretion Mediated by the Calcium-Sensing Receptor in Rats.
  Akiho Igarashi; Shono Ogasawara; Ryo Takagi; Kazufumi Okada; Yoichi M Ito; Hiroshi Hara; Tohru Hira
  The Journal of nutrition, 151, 5, 1320, 1328, 11 May 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Dietary calcium has been proposed to reduce appetite in human studies. Postprandial satiety is mainly controlled by gut hormones. However, the effect of calcium on appetite and the role of gut hormones remain unclear. OBJECTIVES: We examined whether oral administration of calcium reduces food intake in rats and investigated the underlying mechanism. METHODS: Male Sprague Dawley rats (8-12 wk old) were used after an overnight fastifffng. In a series of 2 trials with 1-wk interval between challenges, food intake was measured 0.5-24 h after oral gavage of a vehicle (saline containing 1.5% carboxymethyl cellulose) as the control treatment, or the vehicle containing various calcium compounds [calcium chloride (CaCl2), calcium carbonate, calcium lactate, in a random order] at 150 mg calcium/kg dose. A conditional taste aversion test was conducted. In separate experiments, plasma calcium and gut hormone concentrations were measured 15 or 30 min after oral administration of the calcium compounds. In anesthetized rats, portal peptide-YY (PYY) concentrations were measured after intraluminal administration of a liquid meal with or without additional calcium. RESULTS: Oral CaCl2 reduced food intake acutely (30 min, ∼20%, P < 0.05) compared with control rats, without taste aversion. Plasma PYY concentration was higher (100%, P < 0.05) in CaCl2-preloaded rats than in control rats, 15 min after administration. In anesthetized rats, luminal meal + CaCl2 induced a 4-fold higher increase in plasma PYY than the control treatment did. Oral administration of a calcium-sensing receptor (CaSR) agonist suppressed food intake (∼30%, P < 0.05), but CaCl2 and CaSR agonist did not suppress food intake under treatment with a PYY receptor antagonist. Furthermore, the CaSR antagonist attenuated the effect of CaCl2 on food intake. CONCLUSIONS: CaCl2 suppresses food intake partly by increasing CaSR-mediated PYY secretion in rats. Our findings could at least partially explain the satiating effect of calcium., 32209235
• Glucagon-like peptide-1 response to whey protein is less diminished by dipeptidyl peptidase-4 in comparison with responses to dextrin, a lipid and casein in rats.
  Yuki Shimizu; Hiroshi Hara; Tohru Hira
  The British journal of nutrition, 125, 4, 398, 407, 28 Feb. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Although glucose is the best-known nutrient to stimulate glucagon-like peptide-1 (GLP-1) secretion, dietary peptides also potently stimulate GLP-1 secretion. Certain peptide fragments derived from dietary proteins possess dipeptidyl peptidase-4 (DPP-4) inhibitory activity in vitro. Hence, we hypothesised that dietary peptides protect GLP-1 from degradation through attenuating DPP-4 activity in vivo. Here, we compared GLP-1 responses with dietary proteins, a carbohydrate and a lipid (Intralipos) in rats having or not having plasma DPP-4 activity. Plasma GLP-1 concentrations clearly increased by oral administration of whey protein (2-4 g/kg), but not by that of dextrin (2-4 g/kg), in control rats (untreated Sprague-Dawley rats and F344/Jcl rats), having DPP-4 activity. In contrast, dextrin administration increased the plasma GLP-1 concentrations as the whey protein administration did, in rats having reduced or no DPP-4 activity (a DPP-4 inhibitor, sitagliptin-treated Sprague-Dawley rats or DPP-4-deficient F344/DuCrl/Crlj rats). DPP-4 inhibition by sitagliptin treatment also enhanced GLP-1 response to Intralipos, and casein, but the treatment did not further enhance GLP-1 response to whey protein. Intestinal GLP-1 content and gastric emptying rate were not associated with differences in GLP-1 responses to test nutrients. The luminal contents from rats administered whey protein decreased DPP-4 activity in vitro. These results suggest that GLP-1 released by dextrin, Intralipos and casein was immediately degraded by DPP-4, while GLP-1 released by whey protein was less degraded. Our study provides novel in vivo evidence supporting the hypothesis that dietary peptides not only stimulate GLP-1 secretion but also inhibit DPP-4 activity to potentiate GLP-1 response., 32209235;32209234;11819850
• Blood Sampling From Rat Ileal Mesenteric Vein Revealed a Major Role of Dietary Protein in Meal-Induced GLP-1 Response.
  Tohru Hira; Madoka Sekishita; Hiroshi Hara
  Frontiers in endocrinology, 12, 689685, 689685, 2021, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, The present study was conducted to examine region-dependent glucagon-like peptide-1 (GLP-1) responses to "meal ingestion" under physiological (conscious and unrestrained) conditions using rats with a catheter inserted into either the portal vein (PV) or the ileal mesenteric vein (ILMV). After recovery from the cannulation surgery, blood samples were collected from either PV or ILMV catheter before and after the voluntary ingestion of test diets. After an AIN-93G standard diet ingestion, GLP-1 concentration was higher in ILMV than in PV, and postprandial responses of peptide-YY (PYY) had similar trend, while that of glucose dependent-insulinotropic polypeptide showed an opposite trend to GLP-1/PYY responses. In a separated experiment, a protein-enriched diet containing casein at 25% wt/wt transiently increased GLP-1 concentration only in ILMV; however, a protein-free diet did not increase GLP-1 concentrations in PV or ILMV. These results indicate that postprandial GLP-1 is immediately released from the distal intestine under physiological conditions, and that dietary protein has a critical role in the enhancement of postprandial GLP-1 response., 32209239;32209235
• Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine.
  Motoshi Okumura; Akihiro Hamada; Fumina Ohsaka; Takeshi Tsuruta; Tohru Hira; Kei Sonoyama
  Pflugers Archiv : European journal of physiology, 472, 10, 1521, 1532, Oct. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Impacts of Low-Digestible Carbohydrates and a Rare Sugar on Enteroendocrine Systems
  比良 徹; 原 博
  ルミナコイド研究 : 日本食物繊維学会会誌, 24, 1, 11, 18, 日本食物繊維学会, Jun. 2020, [Peer-reviewed], [Invited], [Lead author, Corresponding author], [Domestic magazines]
  Japanese
• Water-soluble dietary fibers enhance bioavailability of quercetin and a fiber derived from soybean is most effective after long-term feeding in rats.
  Aphichat Trakooncharoenvit; Seiya Tanaka; Erika Mizuta; Tohru Hira; Hiroshi Hara
  European journal of nutrition, 59, 4, 1389, 1398, Jun. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• What Is GLP-1 Really Doing in Obesity?
  Tohru Hira; Jukkrapong Pinyo; Hiroshi Hara
  Trends in endocrinology and metabolism: TEM, 31, 2, 71, 80, Feb. 2020, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, 32209234;11819850;10695713
• Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
  Reika Yoshitsugu; Keidai Kikuchi; Shota Hori; Hitoshi Iwaya; Masahito Hagio; Hidehisa Shimizu; Tohru Hira; Satoshi Ishizuka
  Lipids in health and disease, 19, 1, 9, 9, 15 Jan. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Oral preload of calcium reduces food intake via enhanced PYY secretion in rats
  Tohru Hira; Shono Ogasawara; Hiroshi Hara
  PROCEEDINGS OF THE NUTRITION SOCIETY, 79, OCE2, E239, E239, 2020
  English, International conference proceedings
• Reply to the Letter: Increased bioavailability of plasma polyphenols via the intestinal fermentation of soybean fibers: a role for gut microbiome?
  Aphichat Trakooncharoenvit; Seiya Tanaka; Erika Mizuta; Tohru Hira; Hiroshi Hara
  European journal of nutrition, 58, 6, 2563, 2563, Sep. 2019, [Peer-reviewed], [International Magazine]
  English
• Enhanced postprandial glucagon-like peptide-1 secretion during obesity development has a protective role against glucose intolerance induction in rats.
  Jukkrapong Pinyo; Tohru Hira; Hiroshi Hara
  The British journal of nutrition, 122, 4, 411, 422, 28 Aug. 2019, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, 11819850
• Continuous feeding of a combined high-fat and high-sucrose diet, rather than an individual high-fat or high-sucrose diet, rapidly enhances the glucagon-like peptide-1 secretory response to meal ingestion in diet-induced obese rats.
  Jukkrapong Pinyo; Tohru Hira; Hiroshi Hara
  Nutrition (Burbank, Los Angeles County, Calif.), 62, 122, 130, Jun. 2019, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, 32209238
• Casein materials show different digestion patterns using an in vitro gastrointestinal model and different release of glucagon-like peptide-1 by enteroendocrine GLUTag cells.
  Yosuke Komatsu; Yasuaki Wada; Hirohisa Izumi; Takashi Shimizu; Yasuhiro Takeda; Tohru Hira; Hiroshi Hara
  Food chemistry, 277, 423, 431, 30 Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Wheat gluten hydrolysate potently stimulates peptide-YY secretion and suppresses food intake in rats.
  Wenya Chen; Tohru Hira; Shingo Nakajima; Hiroshi Hara
  Bioscience, biotechnology, and biochemistry, 82, 11, 1992, 1999, Nov. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal
• Novel Mechanism of Fatty Acid Sensing in Enteroendocrine Cells: Specific Structures in Oxo-Fatty Acids Produced by Gut Bacteria Are Responsible for CCK Secretion in STC-1 Cells via GPR40.
  Tohru Hira; Shono Ogasawara; Asuka Yahagi; Minami Kamachi; Jiaxin Li; Saki Nishimura; Masayoshi Sakaino; Takatoshi Yamashita; Shigenobu Kishino; Jun Ogawa; Hiroshi Hara
  Molecular nutrition & food research, 62, 19, e1800146, Oct. 2018, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal
• Resistant maltodextrin or fructooligosaccharides promotes GLP-1 production in male rats fed a high-fat and high-sucrose diet, and partially reduces energy intake and adiposity
  Tohru Hira; Ryoya Suto; Yuka Kishimoto; Sumiko Kanahori; Hiroshi Hara
  EUROPEAN JOURNAL OF NUTRITION, 57, 3, 965, 979, Apr. 2018, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal, 32209239
• Secretion of GLP-1 but not GIP is potently stimulated by luminal d-Allulose (d-Psicose) in rats.
  Masaki Hayakawa; Tohru Hira; Masako Nakamura; Tetsuo Iida; Yuka Kishimoto; Hiroshi Hara
  Biochemical and biophysical research communications, 496, 3, 898, 903, 02 Feb. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, 32209237
• GLP-1 release and vagal afferent activation mediate the beneficial metabolic and chronotherapeutic effects of D-allulose.
  Yusaku Iwasaki; Mio Sendo; Katsuya Dezaki; Tohru Hira; Takehiro Sato; Masanori Nakata; Chayon Goswami; Ryohei Aoki; Takeshi Arai; Parmila Kumari; Masaki Hayakawa; Chiaki Masuda; Takashi Okada; Hiroshi Hara; Daniel J Drucker; Yuichiro Yamada; Masaaki Tokuda; Toshihiko Yada
  Nature communications, 9, 1, 113, 113, 09 Jan. 2018, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal
• 消化管ホルモンup to date 食品ペプチドを利用した消化管内分泌系への作用
  比良徹; 中島進吾; 原博
  月刊消化器・肝臓内科, 3, 2, 140, 146, (有)科学評論社, 2018, [Invited], [Lead author]
  Japanese
• Impact of difructose anhydride III, raffinose, and fructooligosaccharides on energy intake, gut hormones, and cecal fermentation in rats fed a high-fat and high-sucrose diet.
  Tohru Hira; Kurumi Yanagihara; Toshiki Koga; Keisuke Takahashi; Taizo Nagura; Hirokatsu Uchino; Hiroshi Hara
  Bioscience, biotechnology, and biochemistry, 81, 11, 2186, 2194, Nov. 2017, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, 32209238
• Canagliflozin potentiates GLP-1 secretion and lowers the peak of GIP secretion in rats fed a high-fat high-sucrose diet.
  Tohru Hira; Toshiki Koga; Kazuyo Sasaki; Hiroshi Hara
  Biochemical and biophysical research communications, 492, 2, 161, 165, 14 Oct. 2017, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal
• 食品ペプチドで血糖を制御する
  Tohru HIRA
  KAGAKU TO SEIBUTSU, 55, 5, 296, 298, Japan Society for Bioscience, Biotechnology, and Agrochemistry, May 2017, [Peer-reviewed], [Lead author, Last author, Corresponding author]
  Scientific journal
• Zinc directly stimulates cholecystokinin secretion from enteroendocrine cells and reduces gastric emptying in rats
  Shingo Nakajima; Tohru Hira; Hitoshi Iwaya; Hiroshi Hara
  MOLECULAR AND CELLULAR ENDOCRINOLOGY, 430, C, 108, 114, Jul. 2016, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• The calcium-sensing receptor in intestinal cells
  Tohru Hira; Shingo Nakajima; Hiroshi Hara
  Food and Nutritional Components in Focus, 2016-, 10, 396, 412, Royal Society of Chemistry, 2016, [Invited], [Lead author, Corresponding author]
  English, In book
• Diunsaturated Aldehyde, trans,trans-2,4-Decadienal in the Intestinal Lumen Suppresses Gastric Emptying through Serotonin Signaling in Rats
  Tohru Hira; Asuka Yahagi; Said Nishimura; Masayoshi Sakaino; Takatoshi Yamashita; Hiroshi Hara
  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 63, 37, 8177, 8181, Sep. 2015, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Resistant maltodextrin promotes fasting glucagon-like peptide-1 secretion and production together with glucose tolerance in rats
  Tohru Hira; Asuka Ikee; Yuka Kishimoto; Sumiko Kanahori; Hiroshi Hara
  BRITISH JOURNAL OF NUTRITION, 114, 1, 34, 42, Jul. 2015, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Postprandial glucagon-like peptide-1 secretion is increased during the progression of glucose intolerance and obesity in high-fat/high-sucrose diet-fed rats
  Shingo Nakajima; Tohru Hira; Hiroshi Hara
  BRITISH JOURNAL OF NUTRITION, 113, 9, 1477, 1488, May 2015, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Rice protein hydrolysates stimulate GLP-1 secretion, reduce GLP-1 degradation, and lower the glycemic response in rats
  Yuki Ishikawa; Tohru Hira; Daisuke Inoue; Yukikazu Harada; Hiroyuki Hashimoto; Mikio Fujii; Motoni Kadowaki; Hiroshi Hara
  FOOD & FUNCTION, 6, 8, 2525, 2534, 2015, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Dietary sweet potato (Ipomoea batatas L.) leaf extract attenuates hyperglycaemia by enhancing the secretion of glucagon-like peptide-1 (GLP-1)
  Rika Nagamine; Shiori Ueno; Masahito Tsubata; Kazuya Yamaguchi; Kinya Takagaki; Tohru Hira; Hiroshi Hara; Takanori Tsuda
  FOOD & FUNCTION, 5, 9, 2309, 2316, Sep. 2014, [Peer-reviewed]
  English, Scientific journal
• A nondigestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid, alpha-glucosyl-isoquercitrin, on glucagon-like peptide 1 (GLP-1) secretion in rat intestine and enteroendocrine cells
  Panchita Phuwamongkolwiwat; Tohru Hira; Hiroshi Hara
  MOLECULAR NUTRITION & FOOD RESEARCH, 58, 7, 1581, 1584, Jul. 2014, [Peer-reviewed]
  English, Scientific journal
• Activation of the gut calcium-sensing receptor by peptide agonists reduces rapid elevation of plasma glucose in response to oral glucose load in rats
  Maya Muramatsu; Tohru Hira; Arimi Mitsunaga; Eri Sato; Shingo Nakajima; Yoshiro Kitahara; Yuzuru Eto; Hiroshi Hara
  AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 306, 12, C1099, C1107, Jun. 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Unsaturated aldehydes induce CCK secretion via TRPA1 in STC-1 cells
  Shingo Nakajima; Tohru Hira; Asuka Yahagi; Chigusa Nishiyama; Takatoshi Yamashita; Jun Imagi; Hiroshi Hara
  MOLECULAR NUTRITION & FOOD RESEARCH, 58, 5, 1042, 1051, May 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Fructooligosaccharide augments benefits of quercetin-3-O-beta-glucoside on insulin sensitivity and plasma total cholesterol with promotion of flavonoid absorption in sucrose-fed rats
  Panchita Phuwamongkolwiwat; Takuya Suzuki; Tohru Hira; Hiroshi Hara
  EUROPEAN JOURNAL OF NUTRITION, 53, 2, 457, 468, Mar. 2014, [Peer-reviewed]
  English, Scientific journal
• 松田 朋子、神谷 智康、草場 宣延、高野 晃、池口 主弥、高垣 欣也、石橋 千和、比良 徹、原 博
  Effectiveness of Potato Extract in Suppressing Food Intake and Stimulating Satiety
  薬理と治療, 42, 3, 211, 216, Mar. 2014, [Peer-reviewed]
• Oral Administration of Corn Zein Hydrolysate Stimulates GLP-1 and GIP Secretion and Improves Glucose Tolerance in Male Normal Rats and Goto-Kakizaki Rats
  Noriyuki Higuchi; Tohru Hira; Nao Yamada; Hiroshi Hara
  ENDOCRINOLOGY, 154, 9, 3089, 3098, Sep. 2013, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Activation of gut calcium-sensing receptor by peptide agonists promotes glucose tolerance in rats
  Tohru Hira; Maya Muramatsu; Arimi Mitsunaga; Eri Sato; Shingo Nakajima; Yoshiro Kitahara; Yuzuru Eto; Hiroshi Hara
  FASEB JOURNAL, 27, Apr. 2013
  English, International conference proceedings
• Glycochenodeoxycholic acid promotes proliferation of intestinal epithelia via reduction of cyclic AMP and increase in H2AX phosphorylation after exposure to gamma-rays
  Satoshi Ishizuka; Maiko Shiwaku; Masahito Hagio; Takuya Suzuki; Tohru Hira; Hiroshi Hara
  BIOMEDICAL RESEARCH-TOKYO, 33, 3, 159, 165, Jun. 2012, [Peer-reviewed]
  English, Scientific journal
• Suppressive Effect on Food Intake of a Potato Extract (Potein (R)) Involving Cholecystokinin Release in Rats
  Wenya Chen; Tohru Hira; Shingo Nakajima; Hiroshi Tomozawa; Masahito Tsubata; Kazuya Yamaguchi; Hiroshi Hara
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 76, 6, 1104, 1109, Jun. 2012, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Calcium-sensing receptor mediates dietary peptide-induced CCK secretion in enteroendocrine STC-1 cells
  Shingo Nakajima; Tohru Hira; Hiroshi Hara
  MOLECULAR NUTRITION & FOOD RESEARCH, 56, 5, 753, 760, May 2012, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Acute effect of soybean beta-conglycinin hydrolysate ingestion on appetite sensations in healthy humans
  Tohru Hira; Naomi Mori; Toshihiro Nakamori; Hitoshi Furuta; Kozo Asano; Hitoshi Chiba; Hiroshi Hara
  APPETITE, 57, 3, 765, 768, Dec. 2011, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• The G-protein on cholesterol-rich membrane microdomains mediates mucosal sensing of short-chain fatty acid and secretory response in rat colon
  T. Yajima; R. Inoue; M. Yajima; T. Tsuruta; S. Karaki; T. Hira; A. Kuwahara
  ACTA PHYSIOLOGICA, 203, 3, 381, 389, Nov. 2011, [Peer-reviewed]
  English, Scientific journal
• Potato Extract (Potein) Suppresses Food Intake in Rats through Inhibition of Luminal Trypsin Activity and Direct Stimulation of Cholecystokinin Secretion from Enteroendocrine Cells
  Shingo Nakajima; Tohru Hira; Masahito Tsubata; Kinya Takagaki; Hiroshi Hara
  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59, 17, 9491, 9496, Sep. 2011, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Soybean beta-Conglycinin Bromelain Hydrolysate Stimulates Cholecystokinin Secretion by Enteroendocrine STC-1 Cells to Suppress the Appetite of Rats under Meal-Feeding Conditions
  Md. Kaosar Niaz Bin Sufian; Tohru Hira; Toshihiro Nakamori; Hitoshi Furuta; Kozo Asano; Hiroshi Hara
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75, 5, 848, 853, May 2011, [Peer-reviewed]
  English, Scientific journal
• GLP-1 Secretion in Response to Oral and Luminal Palatinose (Isomaltulose) in Rats
  Tohru Hira; Maya Muramatsu; Masahiro Okuno; Hiroshi Hara
  JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 57, 1, 30, 35, Feb. 2011, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• インクレチン分泌を刺激する食品ペプチド，アミノ酸 消化管内分泌細胞による栄養素認識，Ｎｕｔｒｉｅｎｔ‐ｓｅｎｓｉｎｇの解明と応用に期待
  比良 徹
  化学と生物, 49, 1, 11, 13, Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2011, [Peer-reviewed], [Lead author, Last author, Corresponding author]
  Scientific journal
• The Corn Protein, Zein Hydrolysate, Administered into the Ileum Attenuates Hyperglycemia via Its Dual Action on Glucagon-Like Peptide-1 Secretion and Dipeptidyl Peptidase-IV Activity in Rats
  Taisuke Mochida; Tohru Hira; Hiroshi Hara
  ENDOCRINOLOGY, 151, 7, 3095, 3104, Jul. 2010, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Soybean beta 51-63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cells
  Shingo Nakajima; Tohru Hira; Yuzuru Eto; Kozo Asano; Hiroshi Hara
  REGULATORY PEPTIDES, 159, 1-3, 148, 155, Jan. 2010, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• GLP-1 secretion is enhanced directly in the ileum but indirectly in the duodenum by a newly identified potent stimulator, zein hydrolysate, in rats
  Tohru Hira; Taisuke Mochida; Kyoko Miyashita; Hiroshi Hara
  AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 297, 4, G663, G671, Oct. 2009, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Administration of anti-glucagon-like peptide-2 serum suppresses epithelial cell proliferation of the distal small intestine in weanling rats
  Satoshi Ishizuka; Ayako Inafune; Tohru Hira; Hirohisa Izumi; Kazuhiro Ozawa; Mitsunori Takase; Hiroshi Hara
  BIOMEDICAL RESEARCH-TOKYO, 30, 4, 259, 261, Aug. 2009, [Peer-reviewed]
  English, Scientific journal
• alpha-Lactalbumin Hydrolysate Stimulates Glucagon-Like Peptide-2 Secretion and Small Intestinal Growth in Suckling Rats
  Hirohisa Izumi; Satoshi Ishizuka; Ayako Inafune; Tohru Hira; Kazuhiro Ozawa; Takashi Shimizu; Mitsunori Takase; Hiroshi Hara
  JOURNAL OF NUTRITION, 139, 7, 1322, 1327, Jul. 2009, [Peer-reviewed]
  English, Scientific journal
• Cholecystokinin secretion induced by beta-conglycinin peptone depends on G alpha q-mediated pathways in enteroendocrine cells
  Tohru Hira; Toshihiro Maekawa; Kozo Asano; Hiroshi Hara
  EUROPEAN JOURNAL OF NUTRITION, 48, 2, 124, 127, Mar. 2009, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Calcium-sensing receptor mediates phenylalanine-induced cholecystokinin secretion in enteroendocrine STC-1 cells
  Tohru Hira; Shingo Nakajima; Yuzuru Eto; Hiroshi Hara
  FEBS JOURNAL, 275, 18, 4620, 4626, Sep. 2008, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Peptides derived from dolicholin, a phaseolin-like protein in country beans (Dolichos lablab), potently stimulate cholecystokinin secretion from Enteroendocrine STC-1 cells
  Md. Kaosar Nlaz Bin Suman; Tohru Hira; Kozo Asano; Hiroshi Hara
  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 55, 22, 8980, 8986, Oct. 2007, [Peer-reviewed]
  English, Scientific journal
• Sucrose fatty acid esters suppress pancreatic secretion accompanied by peptide YY release in pancreatico-biliary diverted rats
  Tohru Hira; Katsuhiko Takahashi; Hiroshi Hara
  EXPERIMENTAL PHYSIOLOGY, 92, 4, 687, 694, Jul. 2007, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Pork peptone stimulates cholecystokinin secretion from enteroendocrine cells and suppresses appetite in rats
  M. K. N. B. Sufian; Tohru Hira; Kyoko Miyashita; Takashi Nishi; Kozo Asano; Hiroshi Hara
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70, 8, 1869, 1874, Aug. 2006, [Peer-reviewed]
  English, Scientific journal
• Mouse GPR40 heterologously expressed in Xenopus oocytes is activated by short-, medium-, and long-chain fatty acids
  G Stewart; T Hira; A Higgins; CP Smith; JT McLaughlin
  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 290, 3, C785, C792, Mar. 2006, [Peer-reviewed]
  English, Scientific journal
• Multiple fatty acid sensing mechanisms operate in enteroendocrine cells - Novel evidence for direct mobilization of stored calcium by cytosolic fatty acid
  T Hira; AC Elliott; DG Thompson; RM Case; JT McLaughlin
  JOURNAL OF BIOLOGICAL CHEMISTRY, 279, 25, 26082, 26089, Jun. 2004, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Casein binds to the cell membrane and induces intracellular calcium signals in the enteroendocrine cell: A brief communication
  T Hira; H Hara; F Tomita; Y Aoyama
  EXPERIMENTAL BIOLOGY AND MEDICINE, 228, 7, 850, 854, Jul. 2003, [Peer-reviewed], [Lead author]
  English, Scientific journal
• L-homoarginine suppresses exocrine pancreas in rats
  T Hira; S Ohyama; H Hara
  AMINO ACIDS, 24, 4, 389, 396, 2003, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Dietary protein peptic hydrolysates stimulate cholecystokinin release via direct sensing by rat intestinal mucosal cells
  T Nishi; H Hara; T Hira; F Tomita
  EXPERIMENTAL BIOLOGY AND MEDICINE, 226, 11, 1031, 1036, Dec. 2001, [Peer-reviewed]
  English, Scientific journal
• Dietary protein peptic hydrolysates stimulate cholecystokinin release via direct sensing by rat intestinal mucosal cells
  Takashi Nishi; Hirosh Hara; Tohru Hira; Fusao Tomita
  Experimental Biology and Medicine, 226, 11, 1031, 1036, Dec. 2001, [Peer-reviewed]
  English, Scientific journal
• Endogenous cholecystokinin plays a role in down-regulation of pancreatic amylase independent of dietary carbohydrate in rats
  H Hara; S Ohyama; T Hira
  REGULATORY PEPTIDES, 99, 2-3, 103, 110, Jun. 2001, [Peer-reviewed], [Last author]
  English, Scientific journal
• Characterization of binding between the rat small intestinal brush-border membrane and dietary proteins in the sensory mechanism of luminal dietary proteins
  T Hira; H Hara; F Tomita
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 65, 5, 1007, 1015, May 2001, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Luminal dietary protein, not amino acids, induces pancreatic protease via CCK in pancreaticobiliary-diverted rats
  H Hara; S Ohyama; T Hira
  AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 278, 6, G937, G945, Jun. 2000, [Peer-reviewed], [Last author]
  English, Scientific journal
• Stimulative effect of a casein hydrolysate on exocrine pancreatic secretion that is independent of luminal trypsin inhibitory activity in rats
  T Hira; H Hara; Y Aoyama
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 63, 7, 1192, 1196, Jul. 1999, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Effects of DFA IV in rats: Calcium absorption and metabolism of DFA IV by intestinal microorganisms
  K Saito; T Hira; T Suzuki; H Hara; A Yokota; F Tomita
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 63, 4, 655, 661, Apr. 1999, [Peer-reviewed]
  English, Scientific journal
• Guanidino group is involved in the stimulation of exocrine pancreatic secretion by protamine in normal and chronic bile pancreatic juice-diverted rats
  T Hira; H Hara; T Kasai
  PANCREAS, 18, 2, 165, 171, Mar. 1999, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Stimulation of exocrine pancreatic secretion by soybean trypsin inhibitor does not depend on the masking of luminal trypsin activity in rats that have bile-pancreatic juice diverted into the ileum
  T Hira; H Hara; T Kasai
  PANCREAS, 15, 3, 285, 290, Oct. 1997, [Peer-reviewed], [Lead author]
  English, Scientific journal

• Dietary peptide sensing in enteroendocrine cells
  比良徹, 浦上財団研究報告書, 32, 2025
• 動物実験の新しい展開 食事誘導性肥満モデル、血管カニュレーションモデルを用いた、消化管ホルモンGLP-1の分泌調節に関する研究
  比良 徹, 日本栄養・食糧学会大会講演要旨集, 78回, .153, .153, Apr. 2024
  (公社)日本栄養・食糧学会, Japanese
• Trp-TyrのGLP-1分泌促進作用における関与受容体,および構造活性相関の検討
  船橋周倖; 比良徹; 比良徹, 日本アミノ酸学会学術大会講演要旨集, 18th (CD-ROM), 2024
• ラットにおいて,水溶性の低吸収素材による浸透圧負荷がGLP-1分泌を促進する
  水間志織; 比良徹, 日本栄養・食糧学会東北支部大会およびシンポジウム講演要旨集(CD-ROM), 58th, 2024
• ラットにおいてL-リジンの経口投与はカルシウム感知受容体を介してGLP-1分泌を促進する
  入江明歩; 比良徹, 日本栄養・食糧学会大会講演要旨集, 78th, 2024
• ラットにおいて希少糖アルロースにより生じる回腸の伸展がGLP-1分泌を促進する
  水間志織; 比良徹, 日本栄養・食糧学会大会講演要旨集, 78th, 2024
• GABAとリンゴ酸の単回同時摂取が健康成人のGLP-1分泌誘導および食後血糖応答に及ぼす影響
  野口洋樹; 野口洋樹; 上野翔太; 池永武; 甲田哲之; 原博; 比良徹, アミノ酸研究, 16, 2, 2024
• Importance of Trp-Tyr sequence in peptide-induced GLP-1 secretion
  船橋周倖; 田口颯; 比良徹, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• Peptide structures sensed by GLP-1-producing enteroendocrine cells
  比良徹, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• 「食品タンパク質由来の健康機能性ペプチド研究の最先端」 食品タンパク質・ペプチドによるGLP-1分泌促進とそれによる血糖上昇抑制、体温上昇作用
  比良 徹, 日本栄養・食糧学会大会講演要旨集, 77回, 152, 152, Mar. 2023
  (公社)日本栄養・食糧学会, Japanese
• ラットの胃排出速度への各種香辛料の影響
  土居 悠太; 比良 徹, 日本栄養・食糧学会大会講演要旨集, 77回, 215, 215, Mar. 2023
  (公社)日本栄養・食糧学会, Japanese
• Dietary peptides stimulate GLP-1 secretion and reduce its degradation
  比良徹, アミノ酸研究, 16, 1, 2023
• 大豆タンパク質摂取によるGLP-1シグナルを介した直腸温度上昇作用
  落合 啓太; 岩崎 有作; 比良 徹, 肥満研究, 28, Suppl., 296, 296, Nov. 2022
  (一社)日本肥満学会, Japanese
• ラットにおけるえごま油長期摂食による食事刺激に対するGLP-1分泌への影響
  木村 梨乃; 佐野 杏莉; 比良 徹, 日本栄養・食糧学会大会講演要旨集, 76回, 283, 283, May 2022
  (公社)日本栄養・食糧学会, Japanese
• 腸内分泌細胞におけるGABAとL-Phenylalanineの相乗的なGLP-1分泌促進作用-トウモロコシタンパク質調製物の有効成分の探索
  野口洋樹; 野口洋樹; 甲田哲之; 原博; 比良徹, 日本農芸化学会東北支部大会プログラム・講演要旨集, 2022, 2022
• ラット門脈,腸間膜静脈における食後の血中アミノ酸濃度変動
  桂康次朗; 比良徹, アミノ酸研究, 15, 2, 2022
• GLP-1分泌を促進するジペプチド配列の探索
  田口颯; 村井佳奈; 比良徹, 日本栄養・食糧学会北海道支部大会講演要旨集, 52nd, 2022
• 2-モノアシルグリセロールのCCK分泌活性評価およびその作用機構の解明
  落合 啓太; 廣岡 里菜; 境野 眞善; 竹内 茂雄; 比良 徹, 日本栄養・食糧学会大会講演要旨集, 75回, 57, 57, Jul. 2021
  (公社)日本栄養・食糧学会, Japanese
• ラットにおけるえごま油長期摂取は,食事刺激に対するGLP-1分泌応答および持続性を高める
  木村梨乃; 佐野杏莉; 比良徹; 比良徹, 日本栄養・食糧学会東北支部大会およびシンポジウム講演要旨集, 55th, 2021
• ラットにおけるカルシウムによる食欲抑制に対する食事組成の影響
  原田和馬; 比良徹, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2021, 2021
• GLP-1 responses and intestinal tissue analysis in diet-induced obese rats
  佐野杏莉; 比良徹, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• Dietary peptides module GLP-1 and glucose metabolism
  比良徹, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• ラットへ大豆タンパク質を経口投与すると血中GLP-1濃度が高められ,血中DPP-4活性が低下する
  村井佳奈; 比良徹, アミノ酸研究, 14, 1, 63, 64, 2021
  日本アミノ酸学会, Japanese
• Role of dietary proteins in postprandial gut hormone secretion
  比良徹, 飯島藤十郎記念食品科学振興財団年報, 35, 149, 153, 2020
  飯島藤十郎記念食品科学振興財団, Japanese
• ラットにおける大豆タンパク質経口投与時のGLP-1分泌応答と血中DPP-4活性の低下
  村井佳奈; 比良徹, 日本栄養・食糧学会大会講演要旨集, 74th, 193, 193, 2020
  (公社)日本栄養・食糧学会, Japanese
• 希少糖D‐AlluloseのGLP‐1分泌促進作用と求心性迷走神経を介した摂食・糖代謝調節作用
  岩崎有作; 岩崎有作; 仙度光麻; 出崎克也; 比良徹; 中田正範; 中田正範; 原博; DRUCKER Daniel J; 山田祐一郎; 徳田雅明; 矢田俊彦; 矢田俊彦, 日本農芸化学会大会講演要旨集(Web), 2019, ROMBUNNO.3B4p09 (WEB ONLY), 05 Mar. 2019
  Japanese
• カルシウム単回経口投与による消化管ホルモン分泌を介した食欲抑制作用機構の検討
  小笠原奨之; 比良徹, 原博, 日本農芸化学会大会講演要旨集(Web), 2019, ROMBUNNO.1B4p12 (WEB ONLY), 05 Mar. 2019
  Japanese
• ラットにおいて経口投与したタンパク質は糖質よりも血中GLP-1濃度を高める
  清水 祐希; 比良 徹; 原 博, アミノ酸研究, 12, 2, 122, 122, Mar. 2019
  日本アミノ酸学会, Japanese
• 肥満誘導食摂取ラットにおけるたんぱく質投与に対するインクレチン分泌の変化
  田中里実; 原博; 比良徹, 日本アミノ酸学会学術大会講演要旨集, 13th, 2, 104, 104, 2019
  日本アミノ酸学会, Japanese
• ラットにおけるD‐アルロースによるGLP‐1分泌促進作用へのセロトニンの関与
  木村夏織; 早川真輝; 比良徹; 飯田哲郎; 岸本由香; 原博, 日本栄養・食糧学会大会講演要旨集, 73rd, 267, 267, 2019
  (公社)日本栄養・食糧学会, Japanese
• 消化管内分泌細胞における伸展刺激負荷によるGLP‐1分泌応答
  木村夏織; 比良徹, 原博, 日本農芸化学会東北支部大会プログラム・講演要旨集, 153rd, 59, 22 Sep. 2018
  Japanese
• 各種ケルセチン配糖体摂取による消化管ホルモンGLP‐1分泌能の適応的増強現象
  長瀬僚介; 比良徹, 原博, 日本食品科学工学会大会講演集, 65th, 79, 22 Aug. 2018
  Japanese
• 希少糖アルロースの〈GLP‐1内分泌→求心性迷走神経→脳〉軸を介した過食・肥満・糖尿病改善作用
  岩崎有作; 岩崎有作; 仙度光麻; 出崎克也; 比良徹; 佐藤雄大; 中田正範; 中田正範; GOSWAMI Chayon; GOSWAMI Chayon; KUMARI Parmila; 増田千明; 岡田尚巳; 原博; DRUCKER Daniel J; 山田祐一郎; 徳田雅明; 矢田俊彦; 矢田俊彦, 日本糖質学会年会要旨集, 37th, 110, 20 Aug. 2018
  Japanese
• 希少糖アルロースによるGLP-1放出を介した耐糖能向上 インスリン感受性と初期分泌の亢進
  岩崎 有作; 仙度 光麻; 出崎 克也; 比良 徹; 佐藤 雄大; 原 博; 山田 祐一郎; 徳田 雅明; 矢部 大介; 矢田 俊彦, 糖尿病, 61, Suppl.1, S, 322, Apr. 2018
  (一社)日本糖尿病学会, Japanese
• 難消化性糖質の消化管内分泌系への作用
  比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 72回, 154, 154, Apr. 2018
  (公社)日本栄養・食糧学会, Japanese
• ケルセチン配糖体の長期摂取によるGLP‐1分泌能亢進の解析
  長瀬僚介; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 72回, 210, 210, Apr. 2018
  (公社)日本栄養・食糧学会, Japanese
• 小腸腔内のセロトニンはL細胞においてセロトニン受容体HTR4を介してGLP‐1分泌を抑制する
  奥村元; 比良徹; 園山慶, 日本栄養・食糧学会大会講演要旨集, 72回, 187, 187, Apr. 2018
  (公社)日本栄養・食糧学会, Japanese
• 腸内細菌脂質代謝物(修飾脂肪酸)を用いて見出された,CCK分泌を強く促進する脂肪酸の特異的構造
  小笠原奨之; 矢萩明日香; 比良徹; 西村紗希; 山下貴稔; 原博, 日本農芸化学会大会講演要旨集(Web), 2018, ROMBUNNO.2B07p02 (WEB ONLY), 05 Mar. 2018
  Japanese
• 食事中のタンパク質が下部消化管からのGLP-1分泌を誘導する
  関下 まどか; 比良 徹; 原 博, アミノ酸研究, 11, 2, 118, 119, Mar. 2018
  日本アミノ酸学会, Japanese
• 希少糖D-alluloseのGLP-1分泌と求心性迷走神経を介した過食・肥満・糖尿尿病改善作用
  岩崎有作; 岩崎有作; 仙度光麻; 出崎克也; 比良徹; 中田正範; 中田正範; 原博; DRUCKER Daniel J.; 山田祐一郎; 徳田雅明; 矢田俊彦; 矢田俊彦, 日本フードファクター学会学術集会講演要旨集, 23rd, 2018
• 希少糖アルロースによるGLP-1放出を介した耐糖能向上:インスリン感受性と初期分泌の亢進
  岩崎有作; 仙度光麻; 出崎克也; 比良徹; 佐藤雄大; 原博; 山田祐一郎; 徳田雅明; 矢部大介; 矢田俊彦; 矢田俊彦, 糖尿病(Web), 61, Suppl, 2018
• Zein加水分解物による小腸部位別でのGLP‐1分泌応答
  清水祐希; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 72nd, 194, 194, 2018
  (公社)日本栄養・食糧学会, Japanese
• 肥満誘導食摂取ラットにおけるたんぱく質投与に対するGLP‐1分泌応答の変化
  田中里実; 比良徹, 原博, 日本栄養・食糧学会東北支部大会およびシンポジウム講演要旨集, 52nd, 27, 2018
  Japanese
• ラットにおいて経口投与したタンパク質は糖質よりも血中GLP‐1濃度を高める
  清水祐希; 比良徹, 原博, 日本アミノ酸学会学術大会講演要旨集, 12th, 2, 35, 122, 2018
  日本アミノ酸学会, Japanese
• ケルセチン配糖体の反復投与は糖負荷後のラットGLP‐1分泌能を増強する
  長瀬僚介; 比良徹, 原博, 日本未病システム学会学術総会抄録集, 24回, 85, 85, Oct. 2017
  (一社)日本未病学会, Japanese
• 希少糖AlluloseのGLP‐1放出と求心性迷走神経を介した過食・肥満・糖尿病改善
  矢田俊彦; 岩崎有作; 仙度光麻; 比良徹; 佐藤雄大; GOSWAMI Chayon; 原博; 山田祐一郎; 徳田雅明; 出崎克也, 肥満研究, 23, Suppl., 187, 187, Sep. 2017
  (一社)日本肥満学会, Japanese
• ラットへのカルシウム単回経口投与は食欲を抑制する
  小笠原奨之; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 71st, 332, 28 Apr. 2017
  Japanese
• 肥満誘導食摂取ラットにおける食事刺激に対するGLP‐1分泌応答の解析
  柳原くるみ; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 71st, 276, 28 Apr. 2017
  Japanese
• 希少糖D-Alluloseによる糖代謝・摂食調節におけるGLP-1と求心性迷走神経肝臓枝の役割
  矢田 俊彦; 岩崎 有作; 仙度 光麻; Goswami Ghayon; 比良 徹; 原 博; 徳田 雅明; 出崎 克也, 糖尿病, 60, Suppl.1, S, 274, Apr. 2017
  (一社)日本糖尿病学会, Japanese
• 希少糖D‐Allulose摂取によるGLP‐1分泌と求心性迷走神経を介した摂食,糖代謝調節
  岩崎有作; GOSWAMI Chayon; 仙度光麻; 出崎克也; 比良徹; 原博; 徳田雅明; 矢田俊彦, 日本内分泌学会雑誌, 93, 1, 331, 331, Apr. 2017
  (一社)日本内分泌学会, Japanese
• 希少糖D-Alluloseによる糖代謝・摂食調節におけるGLP-1と求心性迷走神経肝臓枝の役割
  矢田俊彦; 岩崎有作; 仙度光麻; GHAYON Goswami; 比良徹; 原博; 徳田雅明; 出崎克也, 糖尿病(Web), 60, Suppl, 2017
• 希少糖アルロースによるGLP‐1放出,求心性迷走神経を介した摂食・糖代謝制御と肥満症改善
  矢田俊彦; 岩崎有作; 比良徹, 原博; GOSWAMI Chayon; 仙度光麻; 徳田雅明; 出崎克也, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 122nd, 108, 2017
  Japanese
• 乳たんぱく質素材の消化性とGLP‐1分泌刺激活性に関する検討
  小松陽介; 小松陽介; 和田泰明; 和田泰明; 井上大輔; 和泉裕久; 清水隆司; 武田安弘; 比良徹, 原博, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.2A04a08 (WEB ONLY), 2017
  Japanese
• 腸管部位別の食後消化管ホルモン分泌変動解析
  関下まどか; 原博, 比良徹, 肥満研究, 23, Supplement, 200, 200, 2017
  (一社)日本肥満学会, Japanese
• 食事中のタンパク質が下部消化管からのGLP‐1分泌を誘導する
  関下まどか; 比良徹, 原博, 日本アミノ酸学会学術大会講演要旨集, 11th, 2, 78, 119, 2017
  日本アミノ酸学会, Japanese
• 肥満誘導食摂取ラットにおけるカナグリフロジン経口投与に対するGLP‐1分泌応答
  比良徹; 古賀俊希; 原博; 石井伸一; 佐々木一代, 肥満研究, 22, Supplement, 215, 20 Sep. 2016
  Japanese
• 希少糖Allulose摂取によるGLP‐1分泌は過食,摂食リズム障害,肥満を改善する
  岩崎有作; KUMARI Parmila; 比良徹; 荒井健; 出崎克也; 原博; 徳田雅明; 矢田俊彦, 肥満研究, 22, Supplement, 182, 20 Sep. 2016
  Japanese
• トウモロコシたんぱく質Zainを含む食事が与える消化管ホルモン分泌応答への影響
  関下まどか; 比良徹, 原博, 日本アミノ酸学会学術大会講演要旨集, 10th, 73, 11 Sep. 2016
  Japanese
• D‐プシコースの経口投与はラットにおいてGLP‐1分泌を促進する
  早川真輝; 比良徹; 飯田哲郎; 岸本由香; 原博, 日本栄養・食糧学会大会講演要旨集, 70th, 267, 25 Apr. 2016
  Japanese
• 糖代謝改善ペプチド
  比良徹, 原博, 日本農芸化学会大会講演要旨集(Web), 2016, 2SY03‐2 (WEB ONLY), 05 Mar. 2016
  Japanese
• ラットにおける肥満誘導食へのDFA III,ラフィノース添加の影響
  古賀俊希; 比良徹; 高橋敬典; 名倉泰三; 内野浩克; 原博, 日本農芸化学会大会講演要旨集(Web), 2016, 4E066 (WEB ONLY), 05 Mar. 2016
  Japanese
• 希少糖によるGLP-1放出,求心性迷走神経を介した摂食抑制と耐糖能向上
  岩崎有作; 比良徹; 出崎克也; 原博; 徳田雅明; 矢田俊彦, 日本病態栄養学会誌(Web), 20, Supplement, 2016
• ゼロカロリー甘味料である希少糖D‐AlluloseはGLP‐1分泌と迷走神経活性化を介して糖尿病・過食・肥満を改善する
  岩崎有作; 荒井健; KUMARI Parmila; 出崎克也; 比良徹; 原博; 徳田雅明; 矢田俊彦, 糖尿病(Web), 59, Suppl, S.450(J‐STAGE), 2016
  Japanese
• 米ペプチドによる消化管ホルモン分泌を介した血糖上昇抑制作用
  比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 70th, 149, 2016
  Japanese
• 亜鉛は消化管内分泌細胞からのCCK分泌を誘導する
  中島進吾; 比良徹; 岩谷一史; 原博, 日本農芸化学会大会講演要旨集(Web), 2016, 4E103 (WEB ONLY), 2016
  Japanese
• たんぱく源の異なる食事による食後ホルモン分泌への影響
  関下まどか; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 70th, 185, 2016
  Japanese
• ラット腸管へのtrans,trans‐2,4‐デカジエナール投与は5‐HT3受容体を介して胃排出を抑制する
  矢萩明日香; 比良徹; 西村紗希; 境野眞善; 山下貴稔; 原博, 肥満研究, 21, Supplement, 210, 08 Sep. 2015
  Japanese
• Luminal trans, trans-2,4-decadienal reduces gastric emptying in rats
  A. Yahagi; T. Hira; S. Nishimura; M. Sakaino; T. Yamashita; H. Hara, DIABETOLOGIA, 58, S305, S305, Sep. 2015
  English, Summary international conference
• Changes in postprandial glucagon-like peptide-1 secretion during the development of diet-induced obesity in rats
  T. Hira; A. Kanehira; H. Hara, DIABETOLOGIA, 58, S271, S271, Sep. 2015
  English, Summary international conference
• trans,trans‐2,4‐デカジエナールは,ラットにおいて腸管への作用を介して胃排出を抑制する
  矢萩明日香; 比良徹; 西村紗希; 境野眞善; 山下貴稔; 原博, 日本農芸化学会大会講演要旨集(Web), 2015, 2F11P18 (WEB ONLY), 05 Mar. 2015
  Japanese
• 米タンパク質の新規生体調節機能性の先導的開発と機構解析 第2章 機能性成分の生体調整機能の研究開発 6 消化管ホルモンGLP‐1分泌促進/分解抑制作用の解明
  原博, 比良徹, 農林水産省農林水産技術会議事務局研究成果, 529, 419, 425, 27 Feb. 2015
  Japanese
• トウモロコシ由来ペプチドにおけるGLP‐1分泌促進機構の解析
  井上大輔; 比良徹, 原博, 日本農芸化学会北海道支部講演会講演要旨, 2015, 19, 2015
  Japanese
• トウモロコシZein由来のGLP‐1分泌促進ペプチドの探索
  山田菜央; 比良徹; 谷口正之; 原博, 日本アミノ酸学会学術大会講演要旨集, 8th, 53, 08 Nov. 2014
  Japanese
• 水溶性難消化性糖質の肥満誘導食への添加はラットのGLP‐1産生を高め,過食,脂肪蓄積を軽減する
  須藤僚也; 比良徹; 岸本由香; 原博, 肥満研究, 20, Supplement, 213, 10 Oct. 2014
  Japanese
• 高脂肪・高ショ糖食摂取ラットにおける食後GLP‐1分泌の解析
  金平葵; 比良徹, 原博, 肥満研究, 20, Supplement, 208, 10 Oct. 2014
  Japanese
• ORAL ADMINISTRATION OF TRANS, TRANS-2,4-DECADIENAL REDUCES GASTRIC EMPTYING IN RATS
  Asuka Yahagi; Tohru Hira; Chigusa Nishiyama; Takatoshi Yamashita; Jun Imagi; Hiroshi Hara, JOURNAL OF MOLECULAR NEUROSCIENCE, 53, S170, S171, Aug. 2014
  English, Summary international conference
• 長期高脂肪・高ショ糖食摂取による絶食時および食後GLP‐1分泌への影響
  金平葵; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 68th, 283, 30 Apr. 2014
  Japanese
• ラットにおいて高脂肪高ショ糖食への難消化性糖質添加は,血中GLP‐1レベルを上昇させ,脂肪蓄積を軽減する
  須藤僚也; 比良徹; 岸本由香; 原博, 日本栄養・食糧学会大会講演要旨集, 68th, 257, 30 Apr. 2014
  Japanese
• 米糠・胚乳ペプチドはラットにおいてGLP‐1分泌を促進し,血中GLP‐1のActive/Total比を高める
  石川結貴; 比良徹; 原田幸和; 橋本博之; 藤井幹夫; 門脇基二; 原博, 日本農芸化学会大会講演要旨集(Web), 2014, 4B05A17 (WEB ONLY), 05 Mar. 2014
  Japanese
• trans,trans‐2,4‐デカジエナールの経口投与によるラット胃排出遅延作用
  矢萩明日香; 比良徹; 西山千草; 山下貴稔; 今義潤, 原博, 日本農芸化学会大会講演要旨集(Web), 2014, 4B05A16 (WEB ONLY), 05 Mar. 2014
  Japanese
• 食品ペプチドの消化管での作用を介した血糖制御に関する研究
  比良徹, アミノ酸研究, 7, 2, 79, 84, Feb. 2014
  Japanese
• 食品ペプチドの消化管での作用を介した血糖制御に関する研究
  比良徹, 日本アミノ酸学会学術大会講演要旨集, 7th, 29, 02 Nov. 2013
  Japanese
• トウモロコシペプチドによるin vivo,in vitroでのGLP‐1分泌作用
  山田菜央; 比良徹, 原博, 日本アミノ酸学会学術大会講演要旨集, 7th, 70, 02 Nov. 2013
  Japanese
• 難消化性デキストリン摂取は,ラット血中GLP‐1濃度を高め,耐糖能を改善する
  池江明日香; 比良徹; 岸本由香; 原博, 肥満研究, 19, Supplement, 189, 25 Sep. 2013
  Japanese
• 消化管内分泌系調節ペプチド
  原博, 比良徹, 日本栄養・食糧学会大会講演要旨集, 67th, 75, 30 Apr. 2013
  Japanese
• ラットにおいて難消化性デキストリンの長期摂取は血中GLP‐1濃度を高める
  池江明日香; 比良徹; 岸本由香; 原博, 日本栄養・食糧学会大会講演要旨集, 67th, 89, 30 Apr. 2013
  Japanese
• ポリリジンはラット腸管において胃排出を遅延し,血糖値上昇を抑制する
  佐藤恵理; 比良徹; 満永有美; 中島進吾; 江藤譲, 原博, 日本栄養・食糧学会大会講演要旨集, 67th, 91, 30 Apr. 2013
  Japanese
• ラットにおいてトウモロコシペプチドの経口投与が,インクレチン分泌促進を介して血糖上昇を抑制する
  比良徹; 樋口謹行; 原博, 糖尿病, 56, Supplement 1, S.197, 25 Apr. 2013
  Japanese
• Fructooligosaccharides augments the effect of a flavonoid, alpha- glucosyl-isoquercitrin, on glucagon-like peptide 1 (GLP-1) secretion
  Panchita Phuwamongkolwiwat; Tohru Hira; Hiroshi Hara, FASEB JOURNAL, 27, Apr. 2013
  English, Summary international conference
• 食欲促進ホルモン・グレリンとその活性化酵素GOATの遺伝子発現にはSiT1/FoxO1/Foxo3aが関与する
  岩崎泰正; 西山充; 田口崇文; 次田誠; 岡崎瑞穂; 中山修一; 江間宏樹; 寺田典生; 藤本新平; 置村康彦; 比良徹; 神林真知子; 川村和夫, 日本内分泌学会雑誌, 89, 1, 274, 01 Apr. 2013
  Japanese
• 消化管内分泌細胞株において不飽和アルデヒドはTRPA1を介してCCK分泌を誘導する
  中島進吾; 比良徹; 西山千草; 山下貴俊; 今義潤, 原博, 日本農芸化学会大会講演要旨集(Web), 2013, WEB ONLY 4A15A02, 05 Mar. 2013
  Japanese
• 米胚乳ペプチドはラットにおいてGLP‐1分泌を促進して血糖上昇を抑制する
  石川結貴; 比良徹; 藤井幹夫; 門脇基二; 原博, 日本農芸化学会大会講演要旨集(Web), 2013, WEB ONLY 4A15A04, 05 Mar. 2013
  Japanese
• Effect of Adzuki Bean Vigna Angularis Cotyledon on Intestinal Alpha-glucosidase Activity and Glucagon-like Peptide-1 Secretion in vitro
  HAMAOKA Naohiro; NAKAGAWA Ryoji; HIRA Tohru; YAMAKI Kohji, Nippon Shokuhin Kagaku Kogaku Kaishi, 60, 1, 43, 47, 15 Jan. 2013
  Japanese
• FRUCTOOLIGOSACCHARIDES ENHANCES THE EFFECT OF A FLAVONOID, alpha-GLUCOSYL-ISOQUERCITRIN, ON GLUCAGON-LIKE PEPTIDE-1 (GLP-1) SECRETION IN RAT INTESTINE AND ENTEROENDOCRINE CELLS
  P. Phuwamongkolwiwat; T. Hira; H. Hara, ANNALS OF NUTRITION AND METABOLISM, 63, 1576, 1576, 2013
  English, Summary international conference
• Diversity of physiological functions of amino acids and peptides PHYSIOLOGICAL FUNCTION OF SOYBEAN PEPTIDES
  H. Hara; S. Nakajima; T. Hira, ANNALS OF NUTRITION AND METABOLISM, 63, 98, 98, 2013
  English, Summary international conference
• Study on glycemic control by luminal dietary peptides
  比良 徹, アミノ酸研究, 7, 2, 79, 84, 2013
  日本アミノ酸学会, Japanese
• 食品ペプチドによるGLP‐1分泌/分解制御機構の解明
  比良徹, 三島海雲記念財団研究報告書, 49, 58, 61, 01 Nov. 2012
  Japanese
• 腸管での食品ペプチド認識と消化管ホルモン分泌に関する研究
  比良徹, 日本農芸化学会北海道支部講演会講演要旨, 2012, 9, 01 Nov. 2012
  Japanese
• 小豆子葉部の高血糖改善機能の評価
  濱岡直裕; 比良徹; 八巻幸二; 中川良二; 太田智樹; 長島浩二, 日本食品化学学会総会・学術大会講演要旨集, 18th, 108, 21 Jun. 2012
  Japanese
• 難消化性デキストリンのラット腸管におけるGLP‐1分泌作用の検討
  池江明日香; 比良徹; 岸本由香; 原博, 日本栄養・食糧学会大会講演要旨集, 66th, 100, 27 Apr. 2012
  Japanese
• トウモロコシZein加水分解物の経口投与はインクレチン分泌を介して血糖上昇を抑制する
  樋口謹行; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 66th, 100, 27 Apr. 2012
  Japanese
• 腸管での栄養素認識と消化管ホルモン分泌に関する研究
  比良徹, 日本栄養・食糧学会大会講演要旨集, 66th, 9, 27 Apr. 2012
  Japanese
• 甘藷若葉「すいおう」のGLP‐1分泌促進作用と血糖値上昇抑制作用
  永峰里花; 上野栞; 田頭英樹; 友澤寛; 鍔田仁人; 比良徹; 原博; 津田孝範, 日本栄養・食糧学会大会講演要旨集, 66th, 219, 27 Apr. 2012
  Japanese
• Fructooligosaccharides (FOS) enhance beneficial effects of a flavonoid, quercetin-3-glucoside (Q3G), on insulin sensitivity in rats fed high sucrose diet
  Panchita Phuwamongkolwiwat; Takuya Suzuki; Tohru Hira; Hiroshi Hara, FASEB JOURNAL, 26, Apr. 2012
  English, Summary international conference
• 甘藷若葉「すいおう」の血糖値上昇抑制作用とGLP‐1分泌への影響
  永峰里花; 上野栞; 友澤寛; 鍔田仁人; 比良徹; 原博; 津田孝範, 日本農芸化学会大会講演要旨集(Web), 2012, 2J12P10 (WEB ONLY), 05 Mar. 2012
  Japanese
• 消化管内分泌細胞におけるカゼインによるCCK分泌機構の解析
  後藤健仁; 比良徹; 中島進吾; 原博, 日本農芸化学会大会講演要旨集(Web), 2012, WEB ONLY 3C29P05, 05 Mar. 2012
  Japanese
• 異なる分子量の食品ペプチドがカルシウム感知受容体を介したCholecystokinin分泌を誘導する
  中島進吾; 比良徹, 原博, 日本農芸化学会大会講演要旨集(Web), 2012, WEB ONLY 3J13A01, 05 Mar. 2012
  Japanese
• Study on Nutrient Sensing and Gut Hormone Secretion:(Young Investigator Award of JSFNS (2012))
  Hira Tohru, Nippon Eiyo Shokuryo Gakkaishi, 65, 5, 215, 220, 2012
  Enteroendocrine cells sense luminal nutrients, but the molecular mechanisms by which dietary peptides induce gut hormone secretions are still unclear. Using a cholecystokinin (CCK)-producing enteroendocrine cell line, it was revealed that the calcium-sensing receptor functions as the receptor for phenylalanine and also for various dietary peptides. Oral administration of a dietary peptide that stimulates CCK secretion reduced both food intake in rats and appetite sensation in humans. Stimulation of glucagon-like peptide-1 (GLP-1) secretion is a promising way to attenuate postprandial hyperglycemia. We found that a dietary peptide prepared from corn zein strongly stimulated GLP-1 secretion in vitro and in vivo. The zein peptide administered into the ileum of rats effectively attenuated the elevation of glycemia through enhancement of GLP-1 secretion and subsequent insulin secretion. The peptide also reduced plasma dipeptidyl peptidase-IV (DPP-IV) activity after intraluminal administration. These findings are important for understanding nutrient-sensing mechanisms in the gut, and also suggest that enhancement of gut hormone secretion by luminal food factors such as dietary peptides could be a novel strategy for control of appetite and glucose metabolism., Japan Society of Nutrition and Food Science, Japanese
• 難消化性デキストリンは消化管内分泌細胞に直接作用してGLP‐1分泌を促進する
  池江明日香; 比良徹; 岸本由香; 原博, ルミナコイド研究, 15, 2, 103-104, 104, 31 Dec. 2011
  Japanese
• トウモロコシたんぱく質Zeinの加水分解物による血糖上昇抑制へのGLP‐1の関与
  樋口謹行; 比良徹, 原博, ルミナコイド研究, 15, 2, 105, 31 Dec. 2011
  Japanese
• ビール酵母抽出物中のCCK分泌促進ペプチドの探索
  須田和希; 比良徹, 原博, 日本アミノ酸学会学術大会講演要旨集, 5th, 71, 04 Nov. 2011
  Japanese
• Calcium‐sensing receptorアゴニストによる血糖上昇抑制とその作用機構の解明
  比良徹; 村松茉耶; 江藤譲, 原博, 肥満研究, 17, Supplement, 190, 01 Sep. 2011
  Japanese
• Soybean beta-Conglycinin Bromelain Hydrolysate Stimulates Cholecystokinin Secretion by Enteroendocrine STC-1 Cells to Suppress the Appetite of Rats under Meal-Feeding Conditions
  Md. Kaosar Niaz Bin Sufian; Tohru Hira; Toshihiro Nakamori; Hitoshi Furuta; Kozo Asano; Hiroshi Hara, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75, 5, 848, 853, May 2011
  English
• 生理活性ペプチド・機能性ペプチド—研究の最先端—消化管ホルモンを介して食欲をコントロールする食品由来ペプチド
  原博; 中島進吾; 比良徹, 臨床化学, 40, 2, 119-126, 126, 28 Apr. 2011
  Japanese
• トウモロコシZein加水分解物はラットへの経口投与によりインクレチン分泌を介して血糖上昇を抑制する
  樋口謹行; 比良徹; 比良徹; 原博, 原博, 日本栄養・食糧学会大会講演要旨集, 65th, 221, 25 Apr. 2011
  Japanese
• 甘藷若葉「すいおう」の糖尿病抑制作用の検討
  史文磊; 待井沙織; 中村早岐; 永峰里花; 鍔田仁人; 高垣欣也; 比良徹; 原博; 津田孝範, 日本栄養・食糧学会大会講演要旨集, 65th, 195, 25 Apr. 2011
  Japanese
• Calcium‐sensing receptorはCCK産生細胞における食品ペプチド受容体として多様なペプチドを認識する
  中島進吾; 比良徹; エムディーカオサルニァビン スフィアン; 江藤譲, 原博, 日本栄養・食糧学会大会講演要旨集, 65th, 193, 25 Apr. 2011
  Japanese
• ビール酵母抽出物中のCCK分泌促進ペプチドの探索とラットでの食欲抑制作用
  須田和希; 比良徹; KAOSAR Sufian; 原博, 日本栄養・食糧学会大会講演要旨集, 65th, 189, 25 Apr. 2011
  Japanese
• 甘藷若葉「すいおう」は耐糖能を改善する
  史文磊; 待井沙織; 中村早岐; 永峰里花; 鍔田仁人; 高垣欣也; 比良徹; 原博; 津田孝範, 日本農芸化学会大会講演要旨集, 2011, 289, 05 Mar. 2011
  Japanese
• ラットにおけるトウモロコシZein加水分解物によるGLP‐1分泌を介した血糖上昇抑制
  比良徹; 持田泰佑; 原博, アミノ酸研究, 4, 2, 163-166, 166, Feb. 2011
  Japanese
• ラットにおけるCalcium‐sensing receptorアゴニストによる血糖上昇抑制作用とその作用機構
  村松茉耶; 比良徹; 江藤譲, 原博, アミノ酸研究, 4, 2, 198-199, 199, Feb. 2011
  Japanese
• Effect of a hydrolysate prepared from soy protein high molecular weight fraction on GLP-1 secretion and glycemia in rats
  比良 徹; 村松 茉耶; 原 博, Soy protein research,Japan, 13, 31, 70, 74, Dec. 2010
  不二たん白質研究振興財団, Japanese
• Effect of a Hydrolysate Prepared from Soy Protein High Molecular Weight Fraction on GLP-1 Secretion and Glycemia in Rats
  HIRA TOORU; MURAMATSU MAYA; HARA HIROSHI, 大豆たん白質研究, 13, 70-74, 74, Dec. 2010
  Japanese
• Corn protein hydrolysate stimulates GLP-1 secretion and attenuates hyperglycaemia in rats
  T. Hira; T. Mochida; H. Hara, REGULATORY PEPTIDES, 164, 1, 43, 43, Sep. 2010
  English, Summary international conference
• Calcium-sensing receptor is a sensor for dietary peptides to stimulate cholecystokinin secretion in the enteroendocrine cell line STC-1
  S. Nakajima; T. Hira; M. K. N. B. Sufian; Y. Eto; H. Hara, REGULATORY PEPTIDES, 164, 1, 45, 45, Sep. 2010
  English, Summary international conference
• 食欲抑制作用を有するジャガイモ抽出物(ポテイン)は消化管ホルモン分泌を強く刺激する
  比良徹; 中島進吾; 鍔田仁人; 高垣欣也; 原博, 肥満研究, 16, Supplement, 170, 01 Sep. 2010
  Japanese
• パラチノースによるGLP‐1分泌促進機構の解析
  比良徹; 村松茉耶; 奥野雅浩; 原博, 肥満研究, 16, Supplement, 171, 01 Sep. 2010
  Japanese
• ポテト抽出物(ポテイン)の満腹感持続作用—朝食併用時の昼食量へ及ぼす影響—
  神谷智康; 松田朋子; 真島明日香; 草場宣廷; 池口主弥; 高垣欣也; 比良徹, 原博, 肥満研究, 16, Supplement, 170, 01 Sep. 2010
  Japanese
• プロタミンはラット腸管においてGLP‐1分泌を刺激し血糖上昇を抑制する
  村松茉耶; 比良徹; 江藤譲, 原博, 日本栄養・食糧学会大会講演要旨集, 64th, 188, 01 May 2010
  Japanese
• ポテインの食欲へ及ぼす効果(1)ヒト長期摂取による抗肥満作用の検証
  真島明日香; 松田朋子; 草場宣廷; 神谷智康; 池口主弥; 高垣欣也; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 64th, 141, 01 May 2010
  Japanese
• CCK産生細胞においてCalcium‐sensing receptorはペプトン受容体として機能する
  中島進吾; 比良徹; SUFAN Kaosar; 江藤譲, 原博, 日本栄養・食糧学会大会講演要旨集, 64th, 210, 01 May 2010
  Japanese
• ポテインの食欲へ及ぼす効果(2)健常成人での単回摂取による空腹感緩和作用の検証
  松田朋子; 真島明日香; 草場宣廷; 神谷智康; 池口主弥; 高垣欣也; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 64th, 141, 01 May 2010
  Japanese
• Identification of Peptides Derived from Azuki Bean (Vigna angularis) which Stimulate Cholecystokinin Secretion from the Enteroendocrine STC-1 Cells
  Md Kaosar Niaz Bin Sufian; Tohru Hira; Hiroshi Hara, FASEB JOURNAL, 24, Apr. 2010
  English, Summary international conference
• ポテインはCCK分泌作用を有し,経口投与によりラットの食欲を抑制する
  中島進吾; 比良徹; 永峰里花; 高垣欣也; 原博, 日本農芸化学会大会講演要旨集, 2010, 49, 05 Mar. 2010
  Japanese
• ポテインの単回および繰り返し摂取による,摂餌量と体重増加抑制作用
  永峰里花; 鮫島まゆ; 池口主弥; 高垣欣也; 比良徹, 原博, 日本農芸化学会大会講演要旨集, 2010, 49, 05 Mar. 2010
  Japanese
• Zein hydrolysate stimulates GLP-1 secretion and attenuates hyperglycemia in rats
  比良 徹; 持田 泰佑; 原 博, Amino acid research, 4, 2, 163, 166, 2010
  日本アミノ酸学会, Japanese
• ポテインの食欲へ及ぼす効果(2)満腹感持続作用
  真島明日香; 草場宣廷; 神谷智康; 池口主弥; 高垣欣也; 比良徹, 原博, 肥満研究, 15, Supplement, 236, 17 Sep. 2009
  Japanese
• ポテインの食欲へ及ぼす効果(1)食事量抑制作用
  鍔田仁人; 杉山大二朗; 鮫島まゆ; 永峰里花; 草場宣廷; 真島明日香; 神谷智康; 池口主弥; 高垣欣也; 比良徹, 原博, 肥満研究, 15, Supplement, 237, 17 Sep. 2009
  Japanese
• トウモロコシZein加水分解物による消化管ホルモンGlucagon‐like peptide‐1分泌機構の解明
  比良徹, 飯島記念食品科学振興財団年報, 2007, 140-144, 144, Aug. 2009
  Japanese
• トウモロコシZein加水分解物はラット腸管においてGLP‐1分泌を刺激して血糖上昇を抑制する
  持田泰佑; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 63rd, 135, 01 May 2009
  Japanese
• 「機能性食品の研究」(第21回)ペプチド栄養学の新展開
  原博, 比良徹, Monthlyフードリサーチ, 647, 62-66, 66, 01 May 2009
  Japanese
• 消化管内分泌細胞株におけるトウモロコシZein加水分解物による消化管ホルモンGlucagon‐like peptide‐1分泌機構
  川村侑輝; 比良徹, 原博, 日本農芸化学会大会講演要旨集, 2009, 208, 05 Mar. 2009
  Japanese
• SHORT CHAIN FATTY ACIDS INDUCE GLP-1 SECRETION IN ENTEROENDOCRINE GLUTAG CELLS
  Tohru Hira; Hiroshi Hara, JOURNAL OF PHYSIOLOGICAL SCIENCES, 59, 389, 389, 2009
  English, Summary international conference
• マウス甘味・うま味受容体T1R3遺伝子の転写調節領域の解析
  豊野孝; 瀬田祐司; 片岡真司; 小田昌史; 比良徹; 豊島邦昭, 日本分子生物学会年会講演要旨集, 32nd, Vol.4, 89, 2009
  Japanese
• 膵腺房細胞タンパク質翻訳開始の制御におけるロイシンとコレシストキニンの相互作用
  瀬戸菜実子; 鈴木卓弥; 比良徹, 原博, アミノ酸研究, 2, 2, 166, Jan. 2009
  Japanese
• 組換え大腸菌による大豆由来生理活性ペプチドの大量生産系構築
  加藤里佳; 白鳥優子; 曾根輝雄; 比良徹; 原博; 浅野行蔵, 日本生物工学会大会講演要旨集, 60th, 117, 11 Jul. 2008
  Japanese
• 大豆由来β51‐63ペプチドはCalcium‐sensing receptorを介してSTC‐1細胞のCholecystokinin分泌を刺激する
  中島進吾; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 62nd, 192, 01 Apr. 2008
  Japanese
• トウモロコシZein加水分解物は,ラット腸管で内分泌細胞への直接的,間接的作用によりGlucagon‐like peptide‐1分泌を刺激する
  持田泰佑; 比良徹, 原博, 日本農芸化学会大会講演要旨集, 2008, 288, 05 Mar. 2008
  Japanese
• フェニルアラニンは,Calcium‐sensing receptorを介してSTC‐1細胞からのCholecystokinin分泌を刺激する
  比良徹, 原博, アミノ酸研究, 1, 1, 51-52, 52, Mar. 2008
  Japanese
• 1Dp18 Production of soybean bioactive peptides by recombinant E. coli.
  KATO Rika; SHIRATORI Yuko; SONE Teruo; HIRA Tohru; HARA Hiroshi; ASANO Kozo, 日本生物工学会大会講演要旨集, 20, 0, 117, 117, 2008
  The Society for Biotechnology, Japan, Japanese
• 野生エゾヒグマの腸管における免疫グロブリンA(IgA)産生細胞の組織分布
  園山慶; 武村直紀; 比良徹; 釣賀一二三; 間野勉, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2008, 18, 2008
  Japanese
• 消化管内分泌細胞株STC‐1において大豆β51‐63ペプチドはCalcium‐sensing receptorを介してCholecystokinin分泌を刺激する
  中島進吾; 比良徹, 原博, 生化学, 4P-0352, 2008
  Japanese
• インスリン分泌を促進する消化管ホルモンGLP‐1の分泌を刺激する食品ペプチドの探索と,消化管における受容機構の解明
  比良徹, 上原記念生命科学財団研究報告集(CD−ROM), 22, ROMBUNNO.33, 2008
  Japanese
• 消化管内分泌細胞株GLUTagにおける,トウモロコシペプチドによる消化管ホルモンGlucagon‐like peptide‐1分泌機構
  川村侑輝; 比良徹, 原博, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2008, 17, 2008
  Japanese
• フェニルアラニンによる消化管内分泌細胞でのCholecystokinin分泌誘導におけるCalcium‐sensing receptorの関与
  比良徹; 中島進吾; 原博, 生化学, 4P-0351, 2008
  Japanese
• 新規評価方法を用いたビールの開発
  三浦裕; 真島紘子; 小泉英樹; 志村雅子; 高山知香子; 西澤祐子; 伏木亨; 比良徹; 原博; 太田雄人, 日本食品科学工学会大会講演集, 54th, 53, 06 Sep. 2007
  Japanese
• Cell signaling pathways for activation of enteroendocrine cells by food peptides: CCK producing cell line, STC-1
  比良 徹; 前川 敏宏; 原 博, Reports of the Research Committee of Essential Amino Acids(Japan), 0, 178, 20, 27, Jun. 2007
  必須アミノ酸研究委員会, Japanese
• 消化管内分泌細胞における食品ペプチドの認識とその情報伝達
  比良徹; 前川敏宏; 原博, 必須アミノ酸研究, 178, 20-27, 27, Jun. 2007
  Japanese
• α‐カゼインによるSTC‐1細胞からのコレシストキニン分泌にはGαsタンパクが関与する
  谷仲真太郎; 井浦和也; 比良徹, 原博, 日本栄養・食糧学会大会講演要旨集, 61st, 148, 20 Apr. 2007
  Japanese
• トウモロコシzein加水分解物は,ラット腸管において消化管ホルモンGlucagon‐like peptide‐1分泌を刺激する
  比良徹; 宮下京子; 原博, 日本農芸化学会大会講演要旨集, 2007, 237, 05 Mar. 2007
  Japanese
• Mechanism of corn zein hydrolysate-stimulated glucagon-like peptide-1 secretion in rat
  比良 徹, Annual report, 2007, 0, 140, 144, 2007
  飯島記念食品科学振興財団, Japanese
• ラット腸管においてトウモロコシZein加水分解物は,内分泌細胞への直接的,間接的作用により消化管ホルモンGlucagon‐like peptide‐1分泌を刺激する
  持田泰佑; 比良徹, 原博, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2007, 24, 2007
  Japanese
• 消化管内分泌細胞株STC‐1におけるα_s‐カゼイン応答性コレシストキニン分泌機構の解析
  谷仲真太郎; 井浦和也; 比良徹, 原博, 生化学, 3P-0267, 2007
  Japanese
• Food peptides directly stimulate glucagon-like peptide-1 secretion in enteroendocrine L-cell without intracellular Ca signaling
  K. Miyashita; T. Hira; H. Hara, REGULATORY PEPTIDES, 135, 3, 144, 144, Aug. 2006
  English, Summary international conference
• Cholecystokinin-releasing peptide derived from soybean beta-conglycinin suppress appetite in human
  T. Hira; K. Sufian; H. Chiba; K. Asano; H. Hara, REGULATORY PEPTIDES, 135, 3, 129, 129, Aug. 2006
  English, Summary international conference
• Casein sensing mechanisms in the cholecystokinin-producing enteroendocrine cells
  T. Hira; K. Iura; H. Hara, REGULATORY PEPTIDES, 135, 3, 129, 129, Aug. 2006
  English, Summary international conference
• Pork peptone stimulates cholecystokinin secretion from enteroendocrine cells and suppresses appetite in rats
  M. K. N. B. Sufian; Tohru Hira; Kyoko Miyashita; Takashi Nishi; Kozo Asano; Hiroshi Hara, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70, 8, 1869, 1874, Aug. 2006
  English
• 摂食調節ペプチド
  原博; 比良徹; 西隆司; 前川敏宏, 日本栄養・食糧学会総会講演要旨集, 60th, 19, 01 Apr. 2006
  Japanese
• βコングリシニンペプトンはGαqタンパクを介してSTC‐1細胞からのコレシストキニン分泌を刺激する
  比良徹; 前川敏宏; 原博, 日本栄養・食糧学会総会講演要旨集, 60th, 299, 01 Apr. 2006
  Japanese
• カゼインは,消化管内分泌細胞株STC‐1におけるカゼイン感受性を亢進させる
  比良徹, 原博, 日本農芸化学会大会講演要旨集, 2006, 278, 05 Mar. 2006
  Japanese
• 大豆たん白質由来の摂食抑制ペプチド
  原博; 西隆司; 比良徹; 浅野行蔵; 中森俊宏, 日本農芸化学会大会講演要旨集, 2006, SHI144, 05 Mar. 2006
  Japanese
• 消化管ホルモンGlucagon‐like peptide‐1(GLP‐1)の分泌を刺激する食品ペプチドの探索および細胞内情報伝達経路の解析
  宮下京子; 比良徹, 原博, 日本農芸化学会大会講演要旨集, 2006, 275, 05 Mar. 2006
  Japanese
• Mouse GPR40 heterologously expressed in Xenopus oocytes is activated by short-, medium-, and long-chain fatty acids
  G Stewart; T Hira; A Higgins; CP Smith; JT McLaughlin, AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 290, 3, C785, C792, Mar. 2006
  English
• 大豆βコングリシニンペプチドは,ヒトの食欲を抑制する
  比良徹; SUFIAN Kaosar; 千葉仁志; 浅野行蔵; 原博, 肥満研究, 11, Supplement, 147, 20 Sep. 2005
  Japanese
• CCK-secreting enteroendocrine cell lines express the novel fatty acid receptor, Gpr40
  G Stewart; T Hira; A Higgins; C Smith; J McLaughlin, GASTROENTEROLOGY, 128, 4, A98, A98, Apr. 2005
  English, Summary international conference
• グルタミン,アスパラギンはコレシストキニン産生細胞株STC‐1の細胞内カルシウムシグナルを活性化する
  比良徹, 原博, 日本栄養・食糧学会総会講演要旨集, 59th, 283, 01 Apr. 2005
  Japanese
• 大豆β‐コングリシニンペプチドによる消化管からの飽食ホルモン分泌機構の探索
  前川敏宏; 比良徹, 原博, 日本栄養・食糧学会総会講演要旨集, 59th, 83, 01 Apr. 2005
  Japanese
• カゼインはサブユニットにより異なったシグナル経路で小腸内分泌細胞からのコレシストキニン(CCK)放出を刺激する
  井浦和也; 比良徹, 原博, 日本農芸化学会大会講演要旨集, 2005, 303, 05 Mar. 2005
  Japanese
• 大豆β-コングリシニン内在ペプチドの惹起する飽食促進機構の探索
  原 博; 前川 敏宏; 比良 徹, 大豆たん白質研究, 7, 25, 108, 113, Oct. 2004
  不二たん白質研究振興財団, Japanese
• Mechanism of Inducing Satiety Effects by Peptides Derived from Soybean .BETA.-Conglycinin
  HARA HIROSHI; MAEKAWA TOSHIHIRO; HIRA TOORU, 大豆たん白質研究, 7, 108-113, 113, Oct. 2004
  Japanese
• 大豆β‐conglycinin内在ペプチドによるコレシストキニン産生細胞のカルシウムシグナル応答
  前川敏宏; 比良徹, 原博, 日本栄養・食糧学会総会講演要旨集, 58th, 278, 01 Apr. 2004
  Japanese
• 消化管内分泌細胞STC‐1におけるα‐カゼイン認識機構の解析
  比良徹, 原博, 日本農芸化学会大会講演要旨集, 2004, 191, 05 Mar. 2004
  Japanese
• Novel acid fatty acid sensing mechanisms operate in enteroendocrine cells: Calcium release from intracellular stores by cytosolic fatty acid
  Hira Tohru; Elliott Austin C.; Thompson David G.; Case R. Maynard; McLaughlin John T., Proceedings of Annual Meeting of the Physiological Society of Japan, 2004, 0, S116, S116, 2004
  Fatty acids (FA) with at least 12 carbon atoms increase intracellular Ca²⁺ ([Ca²⁺]_i) to stimulate cholecystokinin (CCK) release from enteroendocrine cells (EEC). Using the murine EEC line STC-1, we investigated whether candidate intracellular pathways transduce the FA signal, or whether FA themselves act within the cell to release Ca²⁺ directly from the intracellular store. In STC-1 cells loaded with fura-2, short (3 minutes) exposure of C12 fatty acid, but not C8 or C10, induced a dose-dependent increase in [Ca²⁺]_i, in the presence or absence of extracellular Ca²⁺. Various signaling inhibitors, including IP₃ receptor antagonists, all failed to block FA-induced Ca²⁺ responses. In permeabilized STC-1 cells loaded with the lower affinity Ca²⁺ dye magfura-2, and permeabilized by Streptolysin O, again C12 but not C8 or C10, induced release of stored Ca²⁺ dose dependently. However, 30 minutes exposure to C12 induced a sustained elevation of [Ca²⁺]_i in the presence of extracellular Ca²⁺, but only a transient response in the absence of extracellular Ca²⁺. These results suggest that at least two FA sensing mechanisms operate in enteroendocrine cells: intracellularly, FA (C12) transiently induces Ca²⁺ release from intracellular Ca²⁺ stores. However, they also induce sustained Ca²⁺ entry from the extracellular medium to maintain an elevated [Ca²⁺]_i. [Jpn J Physiol 54 Suppl:S116 (2004)], PHYSIOLOGICAL SOCIETY OF JAPAN
• Fatty acids act directly on intracellular calcium stores to release calcium in the enteroendocrine cell line, STC-1
  T Hira; RM Case; DG Thompson; JT McLaughlin, GUT, 52, A54, A54, Apr. 2003
  English, Summary international conference
• DIETARY PROTEIN INCREASES INTRACELLULAR CALCIUM CONCENTRATION IN CHOLECYSTOKININ PRODUCING ENTEROENDOCRINE CELL LINE STC-1-CORRELATION WITH BINDINGS BETWEEN DIETARY PROTEIN AND STC-1 CELL MEMBRANE
  HIRA Tohru; HARA Hiroshi; TOMITA Fusao; AOYAMA Yoritaka, 消化と吸収, 24, 1, 24, 28, 30 Dec. 2001
  Japanese
• Dietary protein increases intracellular calcium concentration in cholecystokinin producing enteroendocrine cell line STC-1. Correlation with bindings between dietary protein and STC-1 cell membrane.
  HIRA TOORU; HARA HIROSHI; TOMITA FUSAO; AOYAMA YORITAKA, 消化と吸収, 24, 1, 24-28, 28, 30 Dec. 2001
  Japanese
• Dietary protein peptic hydrolysates stimulate cholecystokinin release via direct sensing by rat intestinal mucosal cells
  T Nishi; H Hara; T Hira; F Tomita, EXPERIMENTAL BIOLOGY AND MEDICINE, 226, 11, 1031, 1036, Dec. 2001
  English
• 食品タンパク質は消化管内分泌細胞株STC‐1のカルシウムシグナルを惹起する 細胞膜成分と食品タンパク質結合との相関
  比良徹; 原博; 冨田房男; 青山頼孝, 日本消化吸収学会総会プログラム・講演抄録集, 32nd, 102, 17 Oct. 2001
  Japanese
• ラット小腸刷子縁膜中のカゼイン結合成分の解析 消化管における食品タンパク質受容機構について
  比良徹; 原博; 青山頼孝; 冨田房男, 日本農芸化学会誌, 75, 78, 05 Mar. 2001
  Japanese
• 栄養素に対する消化管ホルモンコレシストキニン産生細胞の適応
  比良徹; 麻田知恵子; 原博; 青山頼孝; 冨田房男, 日本栄養・食糧学会総会講演要旨集, 54th, 146, 01 Apr. 2000
  Japanese
• Protein stimulating excocrine pancreas promotes CCK emission in STC-1, gut endocrine cell stock.
  HIRA TOORU; HARA HIROSHI; AOYAMA YORITAKA, 日本栄養・食糧学会総会講演要旨集, 53rd, 87, 20 Apr. 1999
  Japanese
• Effects of DFA IV in rats: Calcium absorption and metabolism of DFA IV by intestinal microorganisms
  K Saito; T Hira; T Suzuki; H Hara; A Yokota; F Tomita, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 63, 4, 655, 661, Apr. 1999
  English
• Extramedullary succagogue action by casein does no depend on trypsin inhibitive capacity in intestinum tenue in a rat.
  HIRA TOORU; HARA HIROSHI; AOYAMA YORITAKA, 日本栄養・食糧学会総会講演要旨集, 52nd, 57, Mar. 1998
  Japanese
• 大豆タンパクトリプシンインヒビターはFOY305より強力な胆膵液非依存性膵外分泌亢進作用を持つ : 動物
  比良 徹; 原 博; 葛西 隆則, 日本農藝化學會誌, 70, 0, 30, 30, 05 Mar. 1996
  社団法人日本農芸化学会, Japanese
• 合成トリプシンインヒビターは長期胆膵液空腸バイパスラットの膵外分泌を亢進するか? : 動物
  比良 徹; 原 博; 葛西 隆則, 日本農藝化學會誌, 69, 0, 346, 346, 05 Jul. 1995
  社団法人日本農芸化学会, Japanese

• ペプチドの生体調節機能と応用
  比良 徹; 分担執筆; 松井利郎; 監修, 第14章 消化管ホルモン調節ペプチド
  シーエムシー出版, Jul. 2024, 9784781318097, viii, 253p, Japanese
• 食品免疫学事典
  比良 徹, ９章(9-13) 消化管内分泌系を介した作用
  朝倉書店, Nov. 2021, [Contributor]
• 食品免疫学事典
  比良 徹; 原 博, ２章(2-14) 消化管ホルモン
  朝倉書店, Nov. 2021, [Contributor]
• エッセンシャル栄養化学 (KS農学専門書)
  比良 徹, 第３章 体内での栄養素の動態：消化・吸収・分布・代謝・排泄
  講談社, 27 Aug. 2021, 4065238064, 320, Japanese, [Contributor]
• 腸内細菌-宿主のクロストークとこれを修飾する食事要因
  比良 徹; 原 博, 難消化性糖質の消化管内分泌系への作用
  建帛社, May 2019, [Contributor]
• 機能性食品開発のための初期評価試験プロトコール集
  比良 徹; 原 博, ラットを用いた食欲抑制試験、GLUTag 細胞を用いた GLP-1 分泌試験、STC-1 細胞を用いた CCK 分泌試験
  シーエムシー出版, Oct. 2017, [Contributor]
• The Calcium-sensing Receptor in Intestinal Cells / Calcium : Chemistry, Analysis, Function and Effects / Food and Nutritional Components in Focus
  Hira T.; Nakajima S.; Hara H., 396-412
  The Royal Society of Chemistry, Oct. 2015, [Contributor]
• 機能性タンパク質・ペプチドと生体利用
  原 博; 比良 徹, 消化管内分泌系を介して作用する機能性ペプチド
  建帛社, May 2010, [Contributor]
• 腸管細胞機能実験法
  原 博; 西 隆司; 比良 徹, 上皮細胞機能の実験法 コレシストキンン産生細胞株STC-1における細胞内カルシウム濃度変化の測定
  学会出版センター, Oct. 2005, 274, 88-96, [Contributor]

• 化学受容、機械受容を介した消化管ホルモンGLP-1分泌促進機構
  比良 徹
  2026年度 日本農芸化学会東北・北海道支部 合同支部会, 03 Oct. 2026
  [Invited]
• 農産物等由来食品が満腹感を与えるメカニズム ―食事成分による肥満・糖尿病の予防へ―
  比良 徹
  北海道大学創基150周年記念シンポジウム in 東京, 22 Aug. 2026
  [Invited]
• 食品成分の化学受容、機械受容を介した GLP-1 分泌促進機構
  比良 徹
  第80回日本栄養・食糧学会大会 シンポジウム, 17 May 2026, Nominated symposium
• 食事誘導性肥満における栄養素応答性GLP-1分泌の適応
  比良 徹
  第55回日本栄養・食糧学会北海道支部シンポジウム, 22 Sep. 2025
  [Invited]
• 食品タンパク質によるGLP-1分泌促進と体温上昇作用
  比良 徹
  インクレチン研究会2025, 05 Apr. 2025, Oral presentation
  [Invited]
• 動物実験の新しい展開 食事誘導性肥満モデル、血管カニュレーションモデルを用いた、消化管ホルモンGLP-1の分泌調節に関する研究
  比良 徹
  第78回日本栄養・食糧学会大会, 25 May 2024
  [Invited]
• GLP-1産生消化管内分泌細胞が認識するペプチド構造
  比良 徹
  日本農芸化学会2023年度大会, 27 Mar. 2024
  [Invited]
• 希少糖D-アルロースによるGLP-1分泌促進機構
  比良 徹
  第14回日本安定同位体・生体ガス医学応用学会大会, 09 Dec. 2023, Nominated symposium
  [Invited]
• 食品成分による消化管ホルモン分泌の調節
  比良 徹
  第111回日本栄養・食糧学会関東支部シンポジウム, 14 Oct. 2023, Nominated symposium
  [Invited]
• 食品タンパク質・ペプチドによる GLP-1 分泌促進とそれによる血糖上昇抑制、 体温上昇作用
  比良 徹
  第77回日本栄養・食糧学会大会, 13 May 2023, Nominated symposium
• 食品成分による消化管ホルモンの分泌
  比良 徹
  第3回 ファーマラボ EXPO 大阪, 09 Mar. 2023, Public discourse
• 食におけるタンパク質の重要性について
  比良 徹
  令和４年度地域バイオ育成講座講演会, 30 Jan. 2023, Public discourse
  [Invited]
• Adaptive changes in nutrient-induced GLP-1 secretion in diet-induced obese model rats
  Tohru Hira
  22nd IUNS-ICN International Congress of Nutritio, 08 Dec. 2022, Nominated symposium
• 食品ペプチドによるGLP-1分泌促進と分解抑制
  比良 徹
  日本アミノ酸学会第９回夏のシンポジウム, 27 Aug. 2022, Nominated symposium
  [Invited]
• 消化管内分泌系への作用を介する糖代謝改善ペプチド
  比良 徹
  日本農芸化学会2021年度大会, 20 Mar. 2021, Nominated symposium
  [Invited]
• trans,trans-2,4-Decadienal stimulates cholecystokinin secretion in enteroendocrine cells, and suppresses gastric emptying in rats
  Tohru Hira
  International symposium on lipids and food ingredients for health promotion, 12 Mar. 2021, Oral presentation
  [Invited]
• 難消化性糖質の消化管内分泌系への作用
  比良 徹
  日本食物繊維学会第24回学術集会, 02 Nov. 2019, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• " 食品成分による消化管内分泌系のコントロール 主にGLP-1に関して"
  比良 徹
  第98回北海道医学大会生理系分科会・日本生理学会北海道地方会, 06 Oct. 2018, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• 難消化性糖質の消化管内分泌系への作用
  比良 徹; 原 博
  第72回日本栄養・食糧学会大会 共催：Hindgut Club Japan, 12 May 2018, Public symposium
  [Invited]
• GLP-1を増やす食品成分、食事条件
  比良 徹
  平成２９年度 公益社団法人日本農芸化学会 北海道支部第２回講演会および公益社団法人日本栄養・食糧学会 第47回北海道支部大会合同大会, 02 Dec. 2017, Japanese, Nominated symposium
  [Domestic Conference]
• 消化管に働きかけて血糖上昇を抑制する食品ペプチド
  比良 徹
  第３回食科学プラットフォームセミナー, 14 Jul. 2017, Public discourse
  [Invited]
• 食品成分による消化管ホルモン分泌調節
  比良 徹
  第18回日本神経消化器病学会、第6回IBS研究会、第84回消化器心身医学研究会、第10回機能性ディスペプシア研究会 合同学術集会2016, 10 Sep. 2016, Nominated symposium
  [Invited]
• 米ペプチドによる消化管ホルモン分泌を介した血糖上昇抑制作用
  比良徹, 原博
  第７０回日本栄養・食糧学会大会, 15 May 2016, Japanese, Nominated symposium
  [Invited]
• 難消化性デキストリンのチカラ－消化管ホルモンを介した新たな機能－
  HIRA Tohru
  第70回日本栄養・食糧学会大会 ランチョンセミナー, 14 May 2016, Public discourse
  [Invited]
• trans,trans-2,4-デカジエナールの胃排出抑制機構
  比良 徹
  Hindgut Club Japanサテライトシンポジウム, 14 May 2016, Nominated symposium
  [Invited]
• 血糖調節ペプチド
  比良 徹
  日本農芸化学会2015年度大会, 28 Mar. 2016, Nominated symposium
  [Invited]
• 食品成分による消化管ホルモン分泌の制御と作用機構
  比良 徹
  第93回日本生理学会大会, 23 Mar. 2016, Nominated symposium
  [Invited]
• 食事誘導性肥満の発症過程におけるGLP-1分泌応答〜ラットでの食事負荷試験による解析
  比良 徹
  第21回Hindgut club Japanシンポジウム, 05 Dec. 2015, Nominated symposium
  [Invited]
• 食事誘導性肥満モデルラットにおける食後GLP-1分泌応答
  比良 徹
  第２７回夏期油脂・コレステロール研究会, 18 Jul. 2015, Nominated symposium
  [Invited]
• 食品ペプチドによる消化管内分泌系への作用を介した血糖調節
  比良 徹
  北大リサーチ＆ビジネスパークセミナー おいしくてヘルシーな北海道！ ～新たなフードサイエンスとビジネス環境へ～, 26 Nov. 2014, Public discourse
  [Invited]
• Effects of resistant maltodextrin and fructooligosaccharides on fasting GLP-1 levels and glucose tolerance in rats
  Tohru Hira
  10th Vahouny Dietary Fiber Symposium, 28 Mar. 2014, Nominated symposium
• 食品ペプチドの消化管での作用を介した血糖制御に関する研究
  比良徹
  2013年度日本アミノ酸学会 科学・技術賞受賞講演, 02 Nov. 2013, Japanese
  [Invited]
• 食品ペプチドによるGLP-1分泌を介した血糖上昇抑制
  比良 徹
  2013年度 生理学研究所研究会 上皮膜輸送の多層的コントロールによる生体の恒常性維持機構, 26 Aug. 2013, Nominated symposium
  [Invited]
• 消化管内分泌系調節ペプチド
  原博, 比良徹
  第67回日本栄養・食糧学会大会S10-03「ペプチドが拓く健康科学の新しい世界」, 26 May 2013, Japanese

• 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
• 食品科学特論, 2024年, 修士課程, 農学院
• 食品科学特論演習, 2024年, 修士課程, 農学院
• 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
• 栄養化学Ⅱ, 2024年, 学士課程, 農学部
• 生物機能化学実験Ⅳ, 2024年, 学士課程, 農学部
• 生物学Ⅰ, 2024年, 学士課程, 全学教育
• 化学概論, 2024年, 学士課程, 農学部

• 日本農芸化学会
• 日本栄養・食糧学会
• 日本アミノ酸学会
• 日本糖尿病学会
• 日本肥満学会
• 日本食物繊維学会

• 食事誘導性肥満におけるGLP-1分泌応答増大の理解と活用
  科学研究費助成事業
  Apr. 2026 - Mar. 2029
  比良 徹
  日本学術振興会, 基盤研究(B), 北海道大学, Principal investigator, 26K01693
• GLP-1分泌促進と分解抑制の両作用を併せ持つペプチドの創生
  科学研究費助成事業
  Jun. 2024 - Mar. 2026
  比良 徹
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, Principal investigator, 24K21844
• Protective mechanisms of enteroendocrine systems against diet-induced obesity and glucose intolerance
  Grants-in-Aid for Scientific Research
  01 Apr. 2022 - 31 Mar. 2025
  比良 徹
  2023年度は、GLP-1分泌を促進することが知られる脂肪酸であるα-リノレン酸を豊富に含有するえごま油を、【1】食事誘導性肥満モデルラット、および【1】糖尿病モデルラットに長期摂取させて、GLP-1分泌や耐糖能へ与える影響を検討した。
  【1】において、ラードを主とする高脂肪食に対し、一部および大部分をえごま油で置換した高脂肪食をラットに長期摂取させた。その結果、高脂肪食の油脂の大部分をえごま油に置換すると、栄養素応答性のGLP-1分泌が増大する傾向が見られた。食後血糖の改善は見られなかったが、空腹時血糖の改善傾向は観察された。さらに血中脂質濃度の低下作用が見られた。これらの結果から、高えごま油食の長期摂取はGLP-1分泌を増大すること、空腹時の糖代謝や脂質代謝を改善する可能性が示唆された。
  【2】において、糖尿病モデルラット（Goto-Kakizakiラット）において、普通食に含まれる大豆油をえごま油に置換して長期摂取させた。食事負荷試験において、GLP-1分泌応答への影響は見られなかったが、血糖上昇の抑制ならびにインスリン分泌の増大傾向が見られた。また、インスリン負荷試験においては、普通食摂取群に比べてえごま油摂取群の方がインスリン投与により血糖がより低下し、インスリン感受性が改善していることが示唆された。これらの結果より、えごま油摂取は、遺伝的糖尿病モデルにおいて、GLP-1分泌促進には依存せずに、耐糖能を改善することが示唆された。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23K23544
• 食事誘導性肥満・耐糖能異常を防ぐ消化管内分泌系の適応機構
  科学研究費助成事業 基盤研究(B)
  Apr. 2022 - Mar. 2025
  比良 徹
  日本学術振興会, 基盤研究(B), 北海道大学, 22H02277
• Mechanisms underlying enhanced GLP-1 response in diet-induced obesity
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  01 Apr. 2019 - 31 Mar. 2022
  比良 徹
  消化管ホルモンGLP-1は、食事刺激により消化管内分泌細胞から分泌され、インスリン分泌増強や食欲抑制などをもたらす。これまでに、食事誘導性の肥満や耐糖能異常の発症過程で、食後GLP-1分泌が増大することを見出した。しかし、消化管および消化管内分泌細胞にどのような変化が生じているのかは不明であり、本研究課題は、これを詳細に解析し、その分子メカニズムを明らかにすることを目的とする。
  はじめに、遺伝的糖尿病モデルラット（Goto-Kakizakiラット）と正常ラットそれぞれに、普通食または高脂肪高ショ糖食を長期間（６ヶ月）摂取させ、２または４週おきに食事負荷試験を実施した。食事負荷試験の際は、全ての群において経腸栄養剤を一定量経口投与した。糖尿病モデルラットと正常ラットともに、高脂肪高ショ糖食の長期摂取により耐糖能不全が生じたが、その程度は糖尿病モデルラットの方が顕著であった。食後GLP-1分泌は、正常ラットでは高脂肪高ショ糖食の持続摂取により増大し、その増大は実験終了時（６ヶ月後）まで維持された。一方で糖尿病モデルラットではそのような増大は見られなかった。このことから、正常なラットでは長期間の肥満誘導食の持続摂取でも食後GLP-1分泌応答は増大すること、そのような適応は遺伝的糖尿病モデルでは生じないことが明らかとなった。食事誘導性肥満におけるGLP-1分泌の増大は、耐糖能の悪化に対して防御的な適応と考えられ、このような適応機構の欠如が糖尿病へと進展する要因の一つであると考えられた。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 19K05860
• Study on postprandial nutrient and gut hormone responses in different intestinal regions
  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
  29 Jun. 2018 - 31 Mar. 2020
  Hara Hiroshi
  Changes in plasma nutrient (glucose) levels and gut hormone levels after meal, in different intestinal regions have not been well characterized.
  We established experimental rat model equipped with a catheter inserted into the ileal mesenteric vein, or the portal vein. After the oral administration of meal having different compositions, plasma glucose levels and gut hormone levels had different responses, dependent on the meal composition, and on the intestinal region of blood sampling.
  These results demonstrate that the rat model is available for investigating regional nutrient absorption and gut hormone secretion under a physiological condition.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), 18K19158
• Study on physiological effects of isomalto-megalo-saccharides
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
  01 Apr. 2017 - 31 Mar. 2020
  Hara Hiroshi
  Isomalto-megalo-saccharides are novel saccharides having unique structures. Various health promotive functions are expected due to their specific interactions with food components and with target cells in the gastrointestinal tract.
  In an intestinal epithelial cell model (Caco2), isomalto-megalo-saccharides prevented impairment of gut barrier function by lipid oxidation products.In animal studies using rats, isomalto-megalo-saccharides enhanced intestinal absorption of quercetin. Furthermore, the combination of isomalto-megalo-saccharides and quercetin potently stimulated secretion of a gastrointestinal hormone, GLP-1.
  These results demonstrate beneficial effects of isomalto-megalo-saccharides, as a functional food factor.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), 17H01473
• Regulation of gut nutrient sensing mechanisms in response to dietary intervention
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  01 Apr. 2016 - 31 Mar. 2019
  Hira Tohru
  We have previously demonstrated that continuous feeding of a high fat and high sucrose diet enhanced nutrient-induced GLP-1 secretion in rats. In this research project, we revealed that the high fat diet, rather than the high sucrose diet, has potent impact on enhancement of nutrient-induced GLP-1 secretion. In addition, the adaptive changes in nutrient-induced GLP-1 secretion was rapidly occurred in young rats than adult rats. Furthermore, by conducting continuous administration of GLP-1 receptor antagonist, we found that the increased GLP-1 response has a role to prevent from glucose intolerance during the development of diet-induced obesity.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 16K07725
• Study on sensing mechanism of low-digestible saccharides in nutrient-sensing enteroendocrine cells
  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
  01 Apr. 2016 - 31 Mar. 2018
  Hara Hiroshi
  We examined the effects of indigestible saccharides including rare sugar D-allulose on a gut hormone GLP-1 secretion. Single oral administration of resistant maltodextrin (RMD) or D-allulose potently stimulated GLP-1 secretion in rats. D-allulose had larger and continuous stimulatory effect on GLP-1 secretion than RMD. The GLP-1-stimulating effect was observed by oral and luminal but not by intraperitoneal administration of D-allulose, indicating D-allulose exerted the effect in the intestinal lumen. These results demonstrate important role of enteroendocrine cell to sense indigestible saccharides in gut hormone secretions under a physiological condition.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 16K14918
• 食事条件に対する消化管栄養素感受性の適応機構の解明と制御
  基盤研究(C)
  Apr. 2016 - Mar. 2018
  比良 徹
  文部科学省, Principal investigator, Competitive research funding
• Study on enteroendocrine, nervous and reproductive systems related to diet for preventing metabolic diseases.
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
  01 Apr. 2014 - 31 Mar. 2017
  Hara Hiroshi
  In this project, we investigated gut hormone secretory responses to ‘meal’in the conscious animal model equipped with portal cannula. We have found that gut hormone responses (such as GLP-1, GIP,PYY, Ghrelin) are modified by changing the source of dietary proteins. Furthermore, the effect of dietary protein was modified by the source of carbohydrates in the meal.
  Feeding the diet supplemented with resistant maltodextrin which has GLP-1-releasing activity prevented excess energy intake induced by high fat high sucrose diet.
  Our results suggest that postprandial gut hormone responses can be desirably manipulated by changing the composition of a meal.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 26252016
• Role of GLP-1 during the development of diet-induced obesity and glucose impairment
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  01 Apr. 2013 - 31 Mar. 2016
  HIRA Tohru
  It is unclear how postprandial GLP-1 secretory response is affected during the development of diet-induced obesity. Meal tolerance test (MTT) was conducted in rats fed normal or high-fat/high-sucrose (HF/HS) diet, and postprandial GLP-1, insulin and glycemic response were monitored.
  Postprandial GLP-1 secretion was larger in HF/HS rats than control rats at week 2 and 4, and this was reflected by increased insulin response in HF/HS rats. In the intestinal mucosa, among various nutrient sensing receptors and transporters evaluated, one of fatty acid receptors, FFAR1 had higher mRNA expression in the upper small intestine of HF/HS rats compared to that of control rats.
  These result demonstrates that postprandial GLP-1 secretion is increased in the early period of developing diet-induced obesity, and that increased postprandial GLP-1 response contributes to normalize postprandial glycemic response by enhancing insulin secretion.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 25450159
• Elucidation of mechanisms to avoid chronic inflammation via enteroendocrine system: relationships between immune cells and adipocytes
  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
  01 Apr. 2012 - 31 Mar. 2015
  ISHIZUKA Satoshi; HIRA Tohru
  Diet-induced obesity induces chronic inflammation. At postplandial period, various external antigens other than dietary lipids get into lymph ducts and evoke immune responses. In this study, we evaluated factors in lymph ducts by using lymph-cannulated rats especially at a postplandial period. At several hours after administration of dietary lipids, there was a significant increase of interleukin-6(IL-6) and glucagon-like peptide-1 in the lymph. Gene expression analysis in food-deprived or fed rats indicated that an accumulation of IL-6 producing cells in the intestinal mucosa accompanied by lipid absorption.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 24658114
• Enhancement of dietary peptide-induced GLP-1 secretion by indigestible saccharides
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  01 Apr. 2010 - 31 Mar. 2013
  HARA Hiroshi; HIRA Tohru
  GLP-1 is one of gastrointestinal hormones, and it has potent anti-obese and anti-diabetic effects. In the present project, we investigated whether indigestible peptides or indigestible saccharides are effective to increase GLP-1 secretion in a cell culture model and animal models. We also examined combined effects of indigestible saccharides and a dietary peptide.
  We demonstrated that oral administration of a dietary peptide (corn peptide) potently stimulated GLP-1 secretion and it resulted in reduced glycemic response in normal and diabetic rats.
  Luminal and oral administration of an indigestible saccharide (resistant maltodextrin, RMD) stimulated GLP-1 secretion in rats, and continuous ingestion of RMD also enhanced basal GLP-1 secretion. Furthermore, rats continuously having RMD showed increased GLP-1 secretory response to oral administration the corn peptide. These results revealed potential and combined effects of indigestible saccharides and dietary peptides in vivo.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 22380070
• Searching for dietary protein/peptide sensing receptors in enteroendocrine cells
  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
  2011 - 2012
  HIRA Tohru
  We searched sensor molecules involved in dietary protein/peptide-sensing in enteroendocrine cells. By using enteroendocrine cell models, we found that a TRP channel was highly expressed in CCK-producing cell line.
  It was demonstrated that the TRP channel is involved in alpha-casein-induced CCK secretion. Furthermore, an aldehyde was found to induce CCK secretion through the TRP channel. Oral administration of the aldehyde reduced gastric emptying rate in rats, suggesting the aldehyde directly acts enteroendocrine cell and reduces gastric emptying in vivo.
  In separated experiments, we found that calcium-sensing receptor functions as sensor for various protein hydrolysates, rather than for specific peptides.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 23780124
• Role of CaSR as dietary peptide sensor and search for dietary protein receptor in the gut
  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
  2009 - 2010
  HIRA Tohru
  In this research project, we have investigated the function of calcium-sensing receptor (CaSR) as the receptor for luminal dietary peptides in gastrointestinal endocrine cells. Secretions of a gut hormone CCK induced by various dietary peptides were partially inhibited by the presence of CaSR antagonists. CaSR-expressing kidney cells also responded to various dietary peptides. These results suggest that CaSR is one of the receptor for dietary peptides in the gastrointestinal tract.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 21780118
• Development of anti-obese dietary peptides which functions via gut hormone secretion
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  2007 - 2009
  HARA Hiroshi; HIRA Tohru
  From various dietary peptides, we found that country bean peptide and beer yeast peptide have potent activity to stimulate a satiating gut hormone CCK in enteroeondcrine cell line. Oral preload of beer yeast peptide reduced following food consumption in rats. We also found that a hydrolysate prepared from corn protein Zein stimulates another gut hormone GLP-1. Ileal administration of Zein hydrolysate strongly induced GLP-1 secretion and subsequent insulin secretion in awake rats, which results in the improvement of glucose tolerance. These findings have revealed that dietary peptides are promising factor for prevention of overeating and hyperglycemia via stimulating gut hormone secretions.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 19380071
• 食品タンパク質中の満腹感促進ペプチドの探索と消化管内分泌細胞におけるその受容機構
  科学研究費助成事業 若手研究(B)
  2005 - 2006
  比良 徹
  昨年度の研究において、フジ豆(ツル豆)より調製したペプトン(フジ豆ペプトン)に、食欲抑制作用を有する消化管ホルモンコレシストキニン(CCK)の分泌を強く刺激する活性があることを見いだした。このペプトンが由来するタンパクを明らかにするため、フジ豆たんぱくを等電点沈殿により分画し、各フラクションよりペプトンを調製して、CCK放出活性を調べたところ、等電点5にて沈殿する画分由来のペプトンに強い活性が見られた。この画分には分子量50kDa程のフジ豆の主要タンパクが多く含まれており、プロテインシークエンサーを使ったN末端アミノ酸配列の解析により、Phaseolin様のタンパクであった。Phaseolin由来のペプチドに強いCCK放出活性があることが示唆された。
  これまでの研究においてCCK産生細胞株STC-1からのCCK放出を刺激することが明らかとなったα-カゼインをリガンドとして、アフィニティー樹脂を用い、CCK産生細胞株STC-1の膜可溶化成分より、種々のα-カゼイン結合タンパクを分離した。プロテインシークエンサーにより解読できた一部のタンパクのアミノ酸配列は、インテグリン、オーファンGPCRと相同性を持っていたことから、これらの分子がα-カゼイン受容体である可能性が考えられた。
  また、STC-1細胞における細胞内情報伝達経路の解析により、この細胞のα-カゼイン認識において、Gαsタンパクが関与すること、ナトリウムチャネルを介した脱分極により細胞内カルシウムシグナルが惹起されることが明らかとなった。上記の受容体候補との関係を今後検討する。
  日本学術振興会, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 17780097
• 消化管における未知の食品タンパク質受容体の検索
  科学研究費助成事業 特別研究員奨励費
  2000 - 2002
  比良 徹
  昨年度までにマウス小腸由来の内分泌細胞株STC・1において、カゼインが細胞内カルシウム濃度上昇を引き起こすことを見出した。そのカルシウムの供給源を検討するため、L型カルシウムチャネルブロッカーDiltiazemの影響を調べた。Diltiazemで前処理されたSTC・1細胞において、α・カゼインによる細胞内カルシウム濃度上昇は有意に抑制され、このことから消化管内分泌細胞はα-カゼインを認識して細胞外からカルシウムを導入していることが示唆された。
  タンパク質上のグアニジル基が消化管で認識されることを示唆する結果をもとに、グアニジル基を有するアミノ酸であるホモアルギニンの投与に対する膵臓外分泌機能の応答を検討した。高コレシストキニン(CCK))血症のモデルとして使われる胆膵液除去ラットおよび正常ラットを外科手術により作製し、1週間の回復期間後にホモアルギニン溶液(10mg/rat)を投与し、投与前後の胆膵液を採取して膵酵素分泌を観察した。十二指腸へのホモアルギニン投与は両ラットに有意な変化を引き起こさなかったが、腹腔へのホモアルギニン投与により胆膵液除去ラットにおいてのみ、膵外分泌抑制作用が見られた。胆膵液除去ラットでのホモアルギニン長期投与の影響を調べるため、25%カゼイン、45%カゼイン、45%カゼイン+ホモアルギニン(19g/kg diet)を含む飼料を4日間与えたところ、45%カゼイン食により誘導された膵臓重量、膵酵素含量の増加は、ホモアルギニン添加により抑制された。胆膵液除去ラットにおける膵酵素の基礎分泌レベルの上昇および、高タンパク質食摂取による膵外分泌機能更新は血中CCK濃度上昇が関与すると考えられているので、ホモアルギニンは胆膵液除去および高タンパク質食により上昇したCCKの作用を血中で阻害する作用を持つこと、即ちCCKレセプターアンタゴニストとしての作用を持つことが示唆された。
  日本学術振興会, 特別研究員奨励費, 北海道大学, 00J06657
• 消化管における栄養素認識機構
  Competitive research funding
• Nutirent sensing mechanism in the gut
  Competitive research funding

• GLP-1分泌促進剤
  Patent right, 矢田 俊彦; 岩崎 有作; 原 博; 比良 徹; 岸本 由香; 南 真知子, 学校法人自治医科大学, 国立大学法人北海道大学, 松谷化学工業株式会社
  特願2017-530931, 29 Jul. 2016
  特開2010-138143, 24 Jun. 2010
  特許第6896247号
  202103004169059868
• GLP-1分泌促進剤及び組成物
  Patent right, 比良 徹; 原 博; 小松 陽介; 和泉 裕久, 森永乳業株式会社
  JP2018007005, 26 Feb. 2018
  WO2018-159546, 07 Sep. 2018
  202003005757708812
• 過剰エネルギー摂取抑制用組成物
  Patent right, 比良 徹; 原 博; 名倉 泰三; 高橋 敬典; 奥原 康英; 石川 純嗣, 国立大学法人北海道大学, 日本甜菜製糖株式会社, 株式会社ファンケル
  特願2017-019582, 06 Feb. 2017
  特開2018-039776, 15 Mar. 2018
  201803007704700121
• GLP-1分泌促進用組成物、及び該組成物の製造方法
  Patent right, 比良 徹; 原 博; 藤井 幹夫, 亀田製菓株式会社, 国立大学法人北海道大学
  特願2016-095639, 11 May 2016
  特開2017-203002, 16 Nov. 2017
  特許第6977945号
  201703015130502607
• GLP-1分泌促進剤
  Patent right, 矢田 俊彦; 岩崎 有作; 原 博; 比良 徹; 岸本 由香; 南 真知子, 学校法人自治医科大学, 国立大学法人北海道大学, 松谷化学工業株式会社
  JP2016072269, 29 Jul. 2016
  WO2017-018500, 02 Feb. 2017
  WO2017-018500, 02 Feb. 2017
  201803003241411782
• TRPA1活性化組成物
  Patent right, 比良 徹; 原 博; 西山 千草; 山下 貴稔; 今義 潤, 株式会社J-オイルミルズ
  特願2013-030850, 20 Feb. 2013
  特開2015-231953, 24 Dec. 2015
  特許第6077331号
  20 Jan. 2017
  201703020704050983
• コレシストキニン分泌促進組成物
  Patent right, 原 博; 比良 徹; 西山 千草; 土倉 則子; 山下 貴稔; 今義 潤, 株式会社J-オイルミルズ
  特願2012-202450, 14 Sep. 2012
  特開2015-178459, 08 Oct. 2015
  特許第5951426号
  17 Jun. 2016
  201603003574652790
• TRPA1活性化組成物
  Patent right, 比良 徹; 原 博; 西山 千草; 山下 貴稔; 今義 潤, 株式会社J-オイルミルズ, 国立大学法人北海道大学
  特願2013-030850, 20 Feb. 2013
  特開2015-231953, 24 Dec. 2015
  201603008520388666
• コレシストキニン分泌促進組成物
  Patent right, 原 博; 比良 徹; 西山 千草; 土倉 則子; 山下 貴稔; 今義 潤, 株式会社J-オイルミルズ, 国立大学法人北海道大学
  特願2012-202450, 14 Sep. 2012
  特開2015-178459, 08 Oct. 2015
  201503012181907950
• グルカゴン様ペプチド-1分泌促進剤
  Patent right, 鍔田 仁人; 原 博; 比良 徹, 株式会社東洋新薬, 国立大学法人北海道大学
  特願2011-133898, 16 Jun. 2011
  特開2012-020993, 02 Feb. 2012
  特許第5794678号
  21 Aug. 2015
  201503019720031422
• グルカゴン様ペプチド-1分泌促進剤
  Patent right, 鍔田 仁人; 原 博; 比良 徹, 株式会社東洋新薬, 国立大学法人北海道大学
  特願2011-544281, 02 Dec. 2010
  特許第5721232号
  03 Apr. 2015
  201503090086819696
• コレシストキニン分泌促進剤
  Patent right, 鍔田 仁人; 原 博; 比良 徹, 株式会社東洋新薬, 国立大学法人北海道大学
  特願2011-544280, 02 Dec. 2010
  特許第5674047号
  09 Jan. 2015
  201503084512039326
• コレシストキニン分泌促進組成物
  Patent right, 原 博; 比良 徹; 西山 千草; 土倉 則子; 山下 貴稔; 今義 潤, 株式会社J-オイルミルズ
  JP2013068827, 10 Jul. 2013
  WO2014-041885, 20 Mar. 2014
  WO2014-041885, 20 Mar. 2014
  201603018851889814
• 経口投与により食欲を抑制する素材
  Patent right, 原 博; 比良 徹; 池田 潔昭; 市原 敬司; 若村 香菜子, グリコハム株式会社, 国立大学法人北海道大学
  特願2011-226782, 14 Oct. 2011
  特開2013-082658, 09 May 2013
  201303078826848232
• 糖尿病又は肥満病の予防又は治療剤
  Patent right, 原 博; 比良 徹; 江藤 譲; 安田 礼子, 味の素株式会社, 国立大学法人北海道大学
  特願2009-193368, 24 Aug. 2009
  特開2012-224547, 15 Nov. 2012
  201203001944681210
• 摂食抑制作用を有する豚肉由来ペプチドを含有する組成物
  Patent right, 比良 徹; 原 博; 浅野 行蔵, 国立大学法人北海道大学
  特願2006-058172, 03 Mar. 2006
  特開2007-230978, 13 Sep. 2007
  特許第4929455号
  24 Feb. 2012
  201303023022744710
• グルカゴン様ペプチド−1分泌促進剤
  Patent right, 鍔田 仁人; 原 博; 比良 徹, 株式会社東洋新薬, 国立大学法人北海道大学
  特願2011-133898, 16 Jun. 2011
  特開2012-020993, 02 Feb. 2012
  201203018386818743
• 血中ジペプチジルペプチダーゼ−IV阻害活性およびグルカゴン様ペプチド−1の分泌促進活性を併せ持つ組成物
  Patent right, 原 博; 比良 徹; 浅野 行蔵, 浅野 行蔵
  特願2010-053383, 10 Mar. 2010
  特開2011-182742, 22 Sep. 2011
  201103016347682524
• コレシストキニン分泌促進剤
  Patent right, 鍔田 仁人; 原 博; 比良 徹, 株式会社東洋新薬, 国立大学法人北海道大学
  JP2010071554, 02 Dec. 2010
  WO2011-068149, 09 Jun. 2011
  201303013576442141
• グルカゴン様ペプチド−1分泌促進剤
  Patent right, 鍔田 仁人; 原 博; 比良 徹, 株式会社東洋新薬, 国立大学法人北海道大学
  JP2010071555, 02 Dec. 2010
  WO2011-068150, 09 Jun. 2011
  201303098218118869
• 糖尿病又は肥満病の予防又は治療剤
  Patent right, 原 博; 比良 徹; 江藤 譲, 味の素株式会社, 国立大学法人北海道大学
  JP2009053409, 25 Feb. 2009
  WO2009-107660, 03 Sep. 2009
  201103074012143866
• 摂食抑制作用を有する豚肉由来ペプチドを含有する組成物
  Patent right, 比良 徹; 原 博; 浅野 行蔵, 国立大学法人 北海道大学
  特願2006-058172, 03 Mar. 2006
  特開2007-230978, 13 Sep. 2007
  200903032408045880
• 摂食抑制作用を有するペプチドを含有する組成物
  Patent right, 比良 徹; 廣塚 元彦; 中森 俊宏; 原 博; 浅野 行蔵; 千葉 仁志; 冨田 房男, 有限会社A−HITBio, 国立大学法人 北海道大学, 不二製油株式会社
  JP2006311426, 07 Jun. 2006
  WO2006-132273, 14 Dec. 2006
  200903047179049769