MF研究者総覧

Researcher Database

Advance
Researchers' Page on researchmap
Last Updated :2024/07/25

Researcher Profile and Settings

Master

Affiliation (Master)

  • Faculty of Science　Chemistry　Physical Chemistry

Affiliation (Master)

  • Faculty of Science　Chemistry　Physical Chemistry

researchmap

Profile and Settings

Degree

  • Doctor of Multi-Disciplinary Sciences（2006/03 The University of Tokyo）
  • Master of Multi-Disciplinary Sciences（2003/03 The University of Tokyo）

Profile and Settings

  • Name (Japanese)

    Kageyama

  • Name (Kana)

    Yoshiyuki

  • Name

    200901065766096991

Alternate Names

Achievement

Research Areas

  • Natural sciences / Bio-, chemical, and soft-matter physics
  • Nanotechnology/Materials / Functional solid-state chemistry
  • Life sciences / Bioorganic chemistry
  • Nanotechnology/Materials / Structural/physical organic chemistry
  • Nanotechnology/Materials / Basic physical chemistry

Research Experience

  • 2013/10 - 2017/03 Japan Science and Technology Agency PRESTO Researcher
  • 2009/04 - 北海道大学大学院理学研究院 助教
  • 2007/04 - 2009/03 Tokyo University of Science Faculty of Pharmaceutical Sciences
  • 2007 - 2009 Researcher,Faculty of Pharmaceutical Sciences, Tokyo Univ. of Science
  • 2006/04 - 2007/03 The University of Tokyo Graduate School of Arts and Sciences
  • 2006 - 2007 Researcher,Graduate School of Arts and Sciences, The Univ. of Tokyo
  • 2005 - 2006 Graduate Student,Graduate School of Arts and Sciences, The Univ. of Tokyo

Education

  •        - 2006  The University of Tokyo
  •        - 2003  The University of Tokyo
  •        - 2001  The University of Tokyo  College of Arts and Sciences  Department of Basic Science

Awards

  • 2015/09 Presentation Award at the 9th symposium on biorelevant chemistry, CSJ
     
    受賞者: KAGEYAMA Yoshiyuki
  • 2013/04 Chemical Society of Japan CSJ Presentation Award 2013
     
    受賞者: KAGEYAMA Yoshiyuki

Published Papers

  • 景山 義之
    Colloid & Interface Communications 公益社団法人 日本化学会 コロイドおよび界面化学部会 48 (1) 34 - 37 2023/03/10 
    生命体のような高度な自律機能を誇る分子システムを構築することは、超分子化学の一つの目標である。この分野では、従来、一個の分子機械の設計と合成に関心が向けられてきた。これに対して筆者は、分子機械を集積させることによって、巨視的な自律機能、例えば自律遊泳を実現する分子システムを構築したので報告する。
  • Yoshiyuki Kageyama, Makiko Matsuura, Daisuke Yazaki
    2023/01/25 [Not invited]
     
    Living organisms show self-sustained motion, make behavioural decisions in response to their environment, and replicate themselves in a genetic manner. Recently, nanometre-sized molecular machines have been assembled to realise macroscopic systems that exhibit self-sustaining dynamics. However, it is unclear how such systems can acquire the ability to make decisions in response to their environment. We have previously reported that the behaviour of a light-driven self-oscillating crystal becomes complicated when the driving light is polarised. Here, we reveal by incorporating a theoretical analysis that the apparent complexity is due to the orientation of the crystal relative to the incident light. An additional reason for this complexity is that the components remember the polarity of the preceding light input. Our results provide a new concept, i.e., collaboration between a motor molecule to achieve self-sustaining motion and a responsive machine for storing information to realise self-governed dynamics in a multimolecular architecture.
  • Yoshiyuki Kageyama, Goro Maruta
    2022/11/14 [Not refereed][Not invited]
     
    Dissipative self-assembly is a recently proposed concept in the field of non-equilibrium systems. This concept catches the reader's attention owing to its relationship with life phenomena. Recently, Credi's group has reported the behavior of a directional supramolecular pump that works under continuous light irradiation with the regime of dissipative self-assembly. Despite their remarkable results, the thermodynamic aspects illustrated in their paper have the potential to mislead readers about the chemistry of non-equilibrium systems.
  • Gyeorye Lee, Yoshiyuki Kageyama, Sadamu Takeda
    Bulletin of the Chemical Society of Japan 95 (6) 909 - 921 0009-2673 2022/06/15 [Refereed][Not invited]
  • Kazuma Obara, Yoshiyuki Kageyama, Sadamu Takeda
    Small 2105302 - 2105302 1613-6810 2022/02 [Refereed][Not invited]
     
    https://www.global.hokudai.ac.jp/blog/mobile-molecular-robots-swim-in-water/
  • 景山 義之
    日本応用物理学会 有機分子・バイオエレクトロニクス分科会誌 32 (4) 194 - 204 2021/11/24 [Not refereed][Invited]
  • 景山 義之
    Isotope News 日本アイソトープ協会 (775) 31 - 35 2021/06/01 [Not refereed][Invited]
  • Yoshiyuki Kageyama
    Symmetry 12 (10) 1688 - 1688 2020/10/14 [Refereed][Invited]
     
    The construction of molecular robotic-like objects that imitate living things is an important challenge for current chemists. Such molecular devices are expected to perform their duties robustly to carry out mechanical motion, process information, and make independent decisions. Dissipative self-organization plays an essential role in meeting these purposes. To produce a micro-robot that can perform the above tasks autonomously as a single entity, a function generator is required. Although many elegant review articles featuring chemical devices that mimic biological mechanical functions have been published recently, the dissipative structure, which is the minimum requirement, has not been sufficiently discussed. This article aims to show clearly that dissipative self-organization is a phenomenon involving autonomy, robustness, mechanical functions, and energy transformation. Moreover, the author details the recent experimental results of an autonomous light-driven molecular device that achieves all of these features. In addition, a chemical model of cell-amplification is also discussed to focus on the generation of hierarchical movement by dissipative self-organization. By reviewing this research, it may be perceived that mainstream approaches to synthetic chemistry have not always been appropriate. In summary, the author proposes that the integration of catalytic functions is a key issue for the creation of autonomous microarchitecture.
  • Yoshiyuki Kageyama, Tomonori Ikegami, Shinnosuke Satonaga, Kazuma Obara, Hiroyasu Sato, Sadamu Takeda
    Chemistry (Weinheim an der Bergstrasse, Germany) 2020/03/19 [Refereed][Not invited]
     
    For creation of autonomous microrobots which are able to move under conditions of a constant environment and a constant energy supply, a mechanism for maintenance of mechanical motion with a capacity for self-control is required. This requirement, known as self-organisation, represents the ability of a system to evade equilibrium through formation of a spatio-temporal pattern. Following our previous finding of a self-oscillatory flipping motion of an azobenzene-containing co-crystal, we present here regulation of the flipping motion by a light-receiving sensor molecule in relation to the alignment and role of azobenzene molecules in crystals. In the anisotropic structure, a specific azobenzene molecule acts as a reaction centre for the conversion of light to a mechanical function process, while the other molecules act as modulators for spatio-pattern regulation. The present results demonstrate that autonomously drivable molecular materials can exhibit information-responsive, self-sustainable motion by incorporating stimulus-responsive sensors.
  • Yoshiyuki Kageyama
    ChemPhotoChem 3 327 - 336 2019/06 [Refereed][Invited]
     
    オープンアクセス。 自律運動発現の要件についてまとめたMinireview。 雑誌の趣旨を踏まえ、光照射下での挙動についてまとめている。自己組織化を専門としない化学者に向け、基礎的な内容に焦点を絞って記載。
  • OBARA Kazuma, KAGEYAMA Yoshiyuki
    Chemistry & Education 日本化学会 67 (2) 74 - 75 0386-2151 2019/02 [Not refereed][Invited]
  • 景山義之
    高分子 67 (6) 328‐329 - 329 0454-1138 2018/06 [Not refereed][Invited]
  • Creation of bioinspired molecular assembly which shows continuous motion with self-organization
    KAGEYAMA Yoshiyuki
    News Letter of the Division of Organic Crystals, CSJ (40) 67 - 70 2017/03 [Not refereed][Not invited]
  • 景山義之
    化学と工業 日本化学会 69 (9) 736‐737 - 737 0022-7684 2016/09 [Not refereed][Invited]
  • Tomonori Ikegami, Yoshiyuki Kageyama, Kazuma Obara, Sadamu Takeda
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 55 (29) 8239 - 8243 1433-7851 2016/07 [Refereed][Not invited]
     
    Building a bottom-up supramolecular system to perform continuously autonomous motions will pave the way for the next generation of biomimetic mechanical systems. In biological systems, hierarchical molecular synchronization underlies the generation of spatio-temporal patterns with dissipative structures. However, it remains difficult to build such self-organized working objects via artificial techniques. Herein, we show the first example of a square-wave limit-cycle self-oscillatory motion of a noncovalent assembly of oleic acid and an azobenzene derivative. The assembly steadily flips under continuous blue-light irradiation. Mechanical self-oscillation is established by successively alternating photoisomerization processes and multi-stable phase transitions. These results offer a fundamental strategy for creating a supramolecular motor that works progressively under the operation of molecule-based machines.
  • Yoshiyuki Kageyama, Tomonori Ikegami, Yuta Kurokome, Sadamu Takeda
    CHEMISTRY-A EUROPEAN JOURNAL 22 (25) 8669 - 8675 0947-6539 2016/06 [Refereed][Not invited]
     
    Macroscopic and spatially ordered motions of self-assemblies composed of oleic acid and a small amount of an azobenzene derivative, induced by azobenzene photoisomerization, was previously reported. However, the mechanism of the generation of submillimeter-scale motions by the nanosized structural transition of azobenzene was not clarified. Herein, an underlying mechanism of the motions is proposed in which deprotonation of carboxyl groups in co-operation with azobenzene photoisomerization causes a morphological transition of the self-assembly, which in turn results in macroscopic forceful dynamics. The photoinduced deprotonation was investigated by potentiometric pH titration and FTIR spectroscopy. The concept of hierarchical molecular interaction generating macroscale function is expected to promote the next stage of supramolecular chemistry.
  • 景山 義之
    C & I commun : colloid & interface communication : newsletter from DCSC 日本化学会コロイドおよび界面化学部会 41 (3) 13 - 15 2016 [Not refereed][Invited]
  • Masahiro Mizuno, Taro Toyota, Miki Konishi, Yoshiyuki Kageyama, Masumi Yamada, Minoru Seki
    LANGMUIR 31 (8) 2334 - 2341 0743-7463 2015/03 [Refereed][Not invited]
     
    A new microfluidic process was used to generate unique micrometer-sized hierarchical lipid particles having spherical lipid-core and multilamellar-shell structures. The process includes three steps: (1) formation of monodisperse droplets in a nonequilibrium state at a microchannel confluence, using a phospholipid-containing water-soluble organic solvent as the dispersed phase and water as the continuous phase; (2) dissolution of the organic solvent of the droplet into the continuous phase and concentration of the lipid molecules; and (3) reconstitution of multilamellar lipid membranes and simultaneous formation of a lipid core. We demonstrated control of the lipid particle size by the process conditions and characterized the obtained particles by transmission electron microscopy and microbeam small-angle X-ray scattering analysis. In addition, we prepared various types of core-shell and core-core-shell particles incorporating hydrophobic/hydrophilic compounds, showing the applicability of the presented process to the production of drug-encapsulating lipid particles.
  • Yoshiyuki Kageyama, Tomonori Ikegami, Natsuko Hiramatsu, Sadamu Takeda, Tadashi Sugawara
    SOFT MATTER 11 (18) 3550 - 3558 1744-683X 2015 [Refereed][Not invited]
     
    The nonequilibrium organization of self-assemblies from small building-block molecules offers an attractive and essential means to develop advanced functional materials and to understand the intrinsic nature of life systems. Fatty acids are well-known amphiphiles that form self-assemblies of several shapes. Here, we found that the lengths of helical structures of oleic acid formed in a buffered aqueous solution are dramatically different by the presence or absence of certain amphiphilic carboxylic acids. For example, under the coexistence of a small amount of N-decanoyl-L-alanine, we observed the formation of over 1 centimeter-long helical assemblies of oleate with a regular pitch and radius, whereas mainly less than 100 mm-long helices formed without this additive. Such long helical assemblies are unique in terms of their highly dimensional helical structure and growth dynamics. Results from the real-time observation of self-assembly formation, site-selective small-angle X-ray scattering, high-performance liquid chromatography analysis, and pH titration experiments suggested that the coexisting carboxylates assist in elongation by supplying oleate molecules to a scaffold for oleate helical assembly.
  • Yasar Akdogan, Wei Wei, Kuo-Ying Huang, Yoshiyuki Kageyama, Eric W. Danner, Dusty R. Miller, Nadine R. Martinez Rodriguez, J. Herbert Waite, Songi Han
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53 (42) 11253 - 11256 1433-7851 2014/10 [Refereed][Not invited]
     
    Sessile marine mussels must dry underwater surfaces before adhering to them. Synthetic adhesives have yet to overcome this fundamental challenge. Previous studies of bioinspired adhesion have largely been performed under applied compressive forces, but such studies are poor predictors of the ability of an adhesive to spontaneously penetrate surface hydration layers. In a force-free approach to measuring molecular-level interaction through surface-water diffusivity, different mussel foot proteins were found to have different abilities to evict hydration layers from surfacesa necessary step for adsorption and adhesion. It was anticipated that DOPA would mediate dehydration owing to its efficacy in bioinspired wet adhesion. Instead, hydrophobic side chains were found to be a critical component for protein-surface intimacy. This direct measurement of interfacial water dynamics during force-free adsorptive interactions at solid surfaces offers guidance for the engineering of wet adhesives and coatings.
  • Yasar Akdogan, Wei Wei, Kuo-Ying Huang, Yoshiyuki Kageyama, Eric W. Danner, Dusty R. Miller, Nadine R. Martinez Rodriguez, J. Herbert Waite, Songi Han
    Angew. Chem. 126 (42) 11435 - 11438 2014/08 [Refereed][Not invited]
  • Kageyama Yoshiyuki
    Seibutsu Butsuri 一般社団法人 日本生物物理学会 54 (1) S134  2014
  • Yoshiyuki Kageyama, Naruho Tanigake, Yuta Kurokome, Sachiko Iwaki, Sadamu Takeda, Kentaro Suzuki, Tadashi Sugawara
    CHEMICAL COMMUNICATIONS 49 (82) 9386 - 9388 1359-7345 2013 [Refereed][Not invited]
     
    Submillimetre size self-assemblies composed of oleate and azobenzene derivatives show forceful motions such as screw-type coiling-recoiling motion by photoirradiation.
  • Koh-ichiroh Shohda, Mieko Tamura, Yoshiyuki Kageyama, Kentaro Suzuki, Akira Suyama, Tadashi Sugawara
    SOFT MATTER 7 (8) 3750 - 3753 1744-683X 2011 [Refereed][Not invited]
     
    The amplification of a 1229 bp template DNA that encoded the green fluorescent protein (GFP) was conducted in cell-sized giant vesicles (GVs, phi > 1 mu m) using a real-time polymerase chain reaction (PCR) technique. The proportion of PCR-proceeded GV reached up to 16% of all GVs. The dependence of PCR on GV size was elucidated by flow cytometry analysis.
  • Hiroshi Takahashi, Yoshiyuki Kageyama, Kensuke Kurihara, Katsuto Takakura, Shigeru Murata, Tadashi Sugawara
    CHEMICAL COMMUNICATIONS 46 (46) 8791 - 8793 1359-7345 2010 [Refereed][Not invited]
     
    In water, phosphoric membrane molecule (V(-)) self-assembled to form an anionic giant vesicle, the surface of which served as a catalyst for the autocatalytic formation of V(-) from its membrane precursor (V(star)), and the amplified V(-) produced a new vesicle using the original vesicle as a scaffold.
  • Shin Aoki, Kengo Hanaya, Yoshiyuki Kageyama, Masanori Kitamura
    HETEROCYCLES 82 (2) 1601 - 1601 0385-5414 2010 [Not refereed][Invited]
     
    Previously, we reported the synthesis of a biotin dopamine conjugate (Btn-DA) with a photocleavable 8-quinolinyl benzenesulfonate (QB) linker for the isolation of an intact complex of DA and anti-DA antibody (IgG(1)) (DA-IgG(1) complex). In this work, we synthesized a photocleavable Btn-DA conjugate with a polyethyleneoxy linker to improve the complexation efficiency and the recovery yields of DA-IgG(1) complex. The results of QCM, ELISA, and Western blot analyses indicate that the introduction of polyethyleneoxy linkers improved the efficiency of DA-IgG(1) complexation.
  • Yoshiyuki Kageyama, Ryosuke Ohshima, Kazusa Sakurama, Yoshihisa Fujiwara, Yoshifumi Tanimoto, Yasuyuki Yamada, Shin Aoki
    CHEMICAL & PHARMACEUTICAL BULLETIN 57 (11) 1257 - 1266 0009-2363 2009/11 [Refereed][Not invited]
     
    Pholochemical cleavage reactions of 8-quinolinyl benzenesulfonate derivatives anti related sulfonates in aqueous solutions are reported. The 8-quinolinyl benzenesulfonates undergo photolysis upon photoirradiation at 300-330 nm to give the corresponding 8-quinolinols and benzenesulfonic acids with the product ion of only negligible amounts of by products. The effects of substituent groups of the 8-quinolinyl moiety and the benzene ring on the photolysis reactions were examined. Based on steady-state mechanistic studies using a triplet sensitizer, a triplet quencher, and electron donors, it was suggested that the photolysis proceeds mainly via the homolytic cleavage of S-O bonds in the excited triplet state.
  • Shin Aoki, Nanako Matsuo, Kengo Hanaya, Yasuyuki Yamada, Yoshiyuki Kageyama
    BIOORGANIC & MEDICINAL CHEMISTRY 17 (9) 3405 - 3413 0968-0896 2009/05 [Refereed][Not invited]
     
    The ability of avidin (Avn) to form strong complex with biotin (Btn) is frequently used in the detection and isolation of biomolecules in biochemical, analytical, and medicinal research. The fact that the binding is nealy irreversible, however, constitutes a drawback in term of the isolation and purification of intact biomolecules. We recently found that 8-quinolinyl esters of aromatic or aliphatic sulfonic acids undergo photolysis when irradiated at 300-330 nm in aqueous solution at neutral pH. In this work, a biotin-dopamine (BD) conjugate containing a photocleavable 8-quinolinyl benzenesulfonate (QB) linker, BDQB, was designed and synthesized for use in the efficient recovery of dopamine-protein (e.g., antibody) complexes from an Avn-Btn system. The complexation of BDQB with a primary anti-dopamine antibody (anti-dopamine IgG(1) from mouse) on an Avn-coated plate was confirmed by an enzyme-linked immunosorbent assay ( ELISA) utilizing a secondary antibody (anti-IgG(1) antibody) conjugated with horseradish peroxidase (HRP). Upon the photoirradiation (at 313 nm) of the BDQB-IgG(1) complex, the release of dopamine-IgG(1) complex was confirmed by ELISA. Characterization of the resulting photoreleased dopamine anti-dopamine IgG(1) complex was performed by SDS-PAGE and Western blot. (C) 2009 Elsevier Ltd. All rights reserved.
  • Shin Aoki, Yumiko Tomiyama, Yoshiyuki Kageyama, Yasuyuki Yamada, Motoo Shiro, Eiichi Kimura
    Chem. Asian J. 4 (4) 561 - 573 1861-4728 2009/04/06 [Refereed][Not invited]
     
    Sulfonamide constitutes a ubiquitous functional group that is frequently used in organic chemistry, analytical chemistry, and medicinal chemistry. We report herein on the photolysis of a dansylamide moiety of 1-dansyl-1,4,7,10-tetraazzacyclododecane (N-dansylcyclen, L-2) in the presence of a zinc(II) ion in aqueous solution. By potentiometric pH titrations, the complexation constant for the 1:1 complex of L-2 and Zn2+, logK(s)(ZnL2), in aqueous solution at 25 degrees C with I=0.1 (NaNO3) was determined to be 6.5 +/- 0.1. The structure of the ZnL2 complex was confirmed by single-crystal X-ray diffraction analysis. During fluorescence titrations of L-2 With Zn2+ (irradiation at 308 or 350 nm) in aqueous solution at pH 7.4 (1.0 mM HEPES with I=0.1 (NaNO3)) and 25 degrees C, considerable enhancement in fluorescence emission of the Zn2+ complex of L-2 (ZnL2) was observed, while metal-free 12 exhibited only a negligible emission change upon UV irradiation. It was revealed that this emission enhancement arose from the photoinduced cleavage of a sulfonylamide moiety in ZnL2, yielding the Zn2+-cyclen complex and 5-dimethylaminonaphthalene-1-sulfinic acid, which has a greater quantum yield (0) for fluorescence emission than that of L-2 and ZnL2. For comparison, the photolysis of N-(1-naphthalenesulfonyl)cyclen (L-3) and its Zn2+ complex (ZnL3) under the same conditions (irradiation at 313 nm) gave the corresponding sulfonate (1-naphthylsulfonate). We also describe the results of a photoreversion reaction of cis,syn-cyclobutane thymine photodimer (T[c,s]T) utilizing the photolysis of ZnL2 and ZnL3.
  • Taro Toyota, Katsuto Takakura, Yoshiyuki Kageyama, Kensuke Kurihara, Naoto Maru, Kiyoshi Ohnuma, Kunihiko Kaneko, Tadashi Sugawara
    LANGMUIR 24 (7) 3037 - 3044 0743-7463 2008/04 [Refereed][Not invited]
     
    Population analysis of a system of self-reproducing giant multilamellar vesicles (GMVs) was carried out by means of flow cytometry. The multidimensional distribution of forward light scattering (FS), side light scattering (SS), and fluorescence (FL) intensities originating from each GMV provided information about changes in a population composed of 10(4) vesicles. FS-FL dot plots indicated that, after the addition of the membrane precursor, the size distribution of the newly generated vesicles was nearly the same as that of the original, but the catalyst content was reduced. This result can be interpreted as evidence for the occurrence of the self-reproduction of GMVs. Moreover, the new GMVs recovered the amount of catalyst to the initial value, keeping their size distribution constant, when a solution of the catalyst was added to the new GMVs. These results are the first experimental evidence for a novel phenomenon on GMV size distribution during their self-reproducing cycle.
  • Tamura Mieko, Kageyama Yoshiyuki, Shohda Koh-ichiroh, Suzuki Kentaro, Sugawara Tadashi
    Seibutsu Butsuri 一般社団法人 日本生物物理学会 48 S56  2008
  • Hanae Ueyama, Yoshiyuki Kageyama, Masanori Kitamura, Eiiehi Kimura, Tomoko Okada, Shin Aoki
    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 128 27 - 27 0031-6903 2008 [Refereed][Not invited]
  • Yoshiyuki Kageyama, Taro Toyota, Shigeru Murata, Tadashi Sugawara
    SOFT MATTER 3 (6) 699 - 702 1744-683X 2007 [Refereed][Not invited]
     
    Flow cytometric analysis revealed that the redistribution of components of a hybrid vesicular system composed of amphiphilic nicotinamide and its reduced form occurred associated with the emergence of nonlamellar aggregates that emitted an enhanced blue emission together with a green emission due to exciplex formation.
  • Shohda Koh-ichiroh, Kiga Daisuke, Takinoue Masahiro, Kageyama Yoshiyuki, Sugawara Tadashi, Suyama Akira
    Seibutsu Butsuri 一般社団法人 日本生物物理学会 46 (2) S413  2006
  • Y Kageyama, S Murata
    JOURNAL OF ORGANIC CHEMISTRY 70 (8) 3140 - 3147 0022-3263 2005/04 [Refereed][Not invited]
     
    In the presence of a catalytic amount of 3-butyl-4-methylthiazolium bromide, the reaction of benzaldehydes with azobenzene in dichloromethane containing octanethiol and Et3N gave the corresponding S-octyl thiobenzoates in good yields. The thiolesters were produced by trapping of the 2-benzoylthiazolium salts with the thiol, which were generated through the azobenzene oxidation of the active aldehydes. This is the first example for the thiolester formation mimicking the function of the pyruvate dehydrogenase complex. An electron-withdrawing substituent at the 4-position of benzaldehyde enhanced the reaction rate. The effect of benzaldehyde substituents on the reaction rate was examined quantitatively on the basis of kinetic measurements, leading to a nonlinear correlation of log(k(obs)) with Hammett's substituent constants (a), The origin of the nonlinear Hammett plot was interpreted in terms of a shift in the rate-determining step of the multistep reaction with change of the electronic nature of substituent. Further support for this assumption was given by the observation that the reaction constant (p) of the Hammett plot for the azobenzene substituent effect on the oxidation rate of 4-bromobenzaldehyde was much smaller than that of 4-cyanobenzaldehyde.
  • 井上 正之, 清家 直樹, 中尾 守男, 中川 貴嗣, 景山 義之
    化学と教育 社団法人日本化学会 43 (10) 668 - 668 0386-2151 1995/10/20 [Not refereed][Not invited]

MISC

Books etc

  • MDB技術予測レポート
    景山義之 (Contributor自律的に物質を輸送する分子ロボットへの挑戦と課題)
    日本能率協会MDB 2023/10
  • KAGEYAMA Yoshiyuki (Contributor(Chapter 26) Interplay of photoisomerization and phase transition events provide a working supramolecular motor)
    Springer 2020/09
  • 自然科学実験2019
    景山 義之 (ContributorC4 ものさしで測る分子の大きさと表面圧)
    学術図書出版社 2019/03 (ISBN: 9784780606904) 11頁 
    授業用教科書
  • KAGEYAMA Yoshiyuki, TAKENAKA Yoshiko, HIGASHIGUCHI Kenji (Contributor(Chapter 8) Material Transfer and Spontaneous Motion in Mesoscopic Scale with Molecular Technology)
    Wiley 2018/07 (ISBN: 9783527341634) 187-208
  • 自然科学実験2018
    景山 義之 (ContributorC4 ものさしで測る分子の大きさと表面圧)
    学術図書出版社 2018/03 11頁 
    授業用教科書

Presentations

Association Memberships

  • THE SOCIETY OF POLYMER SCIENCE, JAPAN   日本化学会有機結晶部会   分子科学会   ソフトマター研究会   「細胞を創る」研究会   複合系の光機能研究会   日本化学会生体機能関連化学部会   THE CHEMICAL SOCIETY OF JAPAN   Japanese Society for Cell Synthesis Research   

Research Projects

  • 日本学術振興会：科学研究費助成事業 学術変革領域研究(A)
    Date (from‐to) : 2023/04 -2025/03 
    Author : 景山 義之
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    Date (from‐to) : 2018/06 -2023/03 
    Author : 角五 彰, 景山 義之
     
    生体分子モーターは優れた運動効率、高い比出力を備えた生体由来の発動分子（生体発動分子）である。この発動分子を動力源としたアクチュエーターの開発が各国で盛んに行われている。しかし、依然として実用化への道のりは遠いのが現状である。その理由として発動分子の決定論的な動作が保障されていない、耐久性や熱安定性が低い、ダイナミクスを制御・操作する技術が確立されていないなどの要因がある。本研究では生体分子モーターに人工修飾を加えたハイブリッド型生体発動分子を創出・集積し、集団で機能するエネルギー変換素子の創成を目指す。併せて人工の発動分子の集団エネルギー変換系の構築に取り組み、時空間的階層性を有した分子運動系の基礎学理構築を目指している。本年度（平成 30 年度）には、二次元界面上での生体発動分子（キネシン）の運動発現に取り組み、生体発動分子をエラストマーやリン脂質などから構成されるソフトな二次元界面上に固定化する技術を確立させた。さらに領域内連携によりATPをエネルギー源とした生体発動分子（微小管）の機械的な運動を二次元界面上で発現させ、ロバストな運動のイメージング解析を確立させた。また実験環境下での生体発動分子、特にキネシンの効率的な駆動を実現するために、班員が有する特許（熱安定化変異体のスクリーニング法をもとに熱耐性キネシンの開発に着手した。 同時に人工発動分子の集団エネルギー変換系の学理構築に向け、①既に構築している人工発動分子集合体の自律運動について数理科学者・物理学者との連携研究の開始、および②新たに構築する化学基質駆動型の人工発動分子システムの創出に向けた試料の合成と、その二次元界面への固定化に着手した。
  • 日本学術振興会：科学研究費助成事業 新学術領域研究(研究領域提案型)
    Date (from‐to) : 2020/04 -2022/03 
    Author : 景山 義之
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    Date (from‐to) : 2017/04 -2019/03 
    Author : 景山 義之
     
    物質自体の性質により光照射下で自発的に屈曲運動を繰り返す「アゾベンゼン誘導体含有薄膜状結晶」について、物理化学的あるいは化学物理的考察を推進することが、当該研究である。結晶であるにもかかわらず屈曲することに加え、光定常状態でその屈曲を繰り返す点で、研究大雨小の物質は他に類を見ない物質といえる。 前年度に、アゾベンゼン誘導体(1)を成分とする結晶の暗所での構造解析に成功した。原子密度が疎な層と原子密度が密な層とが交互に積層した結晶構造から、原子密度が疎な相での光異性化が結晶の構造相転移を誘起すること、および原子密度が密な相が結晶の集積状態を保っていること、が推測された。 今年度は、光照射下で形成するもう一つの結晶相の構造解析や過渡的変化の計測を目標に、独ポツダム大学との国際共同研究で、欧州放射光施設でのX線回折実験を試みた。しかし、一時的に形成する結晶を対象にしたX線回折実験は難しく、目標は達成できなかった。計測できなかった要因の一つは、光照射下で形成した屈曲した結晶が、単結晶ではなく、小さな結晶ドメインの集合体であるためではないかと考えている。 この仮説は、構造の異なるアゾベンゼン誘導体(2)が形成する大きめの結晶の光照射下での運動を観察することで、信憑性を帯びてきた。大きめの結晶は、屈曲運動ではなく、波状運動をみせる。これは、結晶が単結晶である場合には説明しにくい、「伝搬」が働く現象である。 すなわち、分子レベルでの可逆な光異性化が微視的な結晶の可逆な相転移を引き起こすこと、微視的結晶の可逆な相転移がマクロな結晶の可逆な構造変化を引き起こすこと、の二段階の自己組織化ダイナミクスが、この「光照射下で自律的に機械振動をみせる結晶」の中で起きていると推察された。これは、多くの化学者が漠然と興味を抱く「自己組織化」現象の基礎的かつ本質的理解を促す成果であり、継続的な研究展開が望まれる。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    Date (from‐to) : 2017/04 -2019/03 
    Author : 景山 義之
     
    分子機械の構造変化を、熱力学の規律が働くマクロスコピックな継続運動へと変換するためには、空間的な対称性の破れが必要である。これに、時間的な対称性の破れ・反応の継続性が加わり、相や場で組織化することにより、マクロなレベルで自律的に動き続ける散逸分子システムを創出可能である。 このコンセプトの確立を目的に、本研究課題では、化学反応で連続駆動する有機金属触媒を、自己配向性を有する液晶分子を用いて集積し、供給したエネルギーを消費しながら自律的に駆動する分子システムを創出する。この研究を通じて、上記仮説の証明と、ミクロとマクロをつなぐ物理的な関係を追究し、自律駆動デバイス・能動的デバイス創出にかかわる基礎学理を構築していく。 平成２９年度は、当初計画に従い、芳香族配位子を有したパラジウム触媒とシアノビフェニル系液晶分子を混合した液晶性触媒反応場を調製し、その反応場を利用した鈴木宮浦カップリング反応を行った。まずは、不斉炭素を持たない基質を用い、反応場の触媒活性を評価した。一般的な溶液系での反応と比較し、触媒反応効率の低下が起きたものの、目的のカップリング反応を進めることはできた。反応効率低下の理由は、反応が起こる表面が少ないこと、液晶分子自体の反応による目的反応の阻害、あるいは、触媒分子の構造変化自由度が液晶分子によって阻害された、などの原因が考えらえるが、三つ目の仮説の場合、触媒分子の構造変化が液晶分子の運動状態に影響を与えていることになる。このほか、基質の反応率に対して目的物の生成量が十分ではないなど、計画申請当初想定していた「研究上の困難点」が現れてきている。 これに伴い、困難が生じた場合の対処法として計画していた、自己集積膜(SAM)などの組織化膜を用いた実験系の準備にも取り掛かり始めた。なお、困難点の発生も含め、研究はほぼ計画通りに進行している。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2016/04 -2019/03 
    Author : KAGEYAMA Yoshiyuki, TAKEDA Sadamu, KONDO Ryota, LEE Gyeorye
     
    Polymers and molecular assemblies exhibited in water (e.g. peptides and bio-membranes), stabilize their structures with the interaction between water and are worked cooperatively with water molecules. The aim of this research project is in developing the method to reveal the dynamic behavior of water surrounding such soft assemblies. In this project, we developed our home-made measurement system for in-situ dynamic nuclear polarization nuclear magnetic resonance, with synthesizing a newly functional probe, which change its structure by light-irradiation. Next step, we will develop the understanding about movement of water molecule around soft materials via discussion about the obtained results with physicists.
  • JST：PRESTO
    Date (from‐to) : 2013/10 -2017/03 
    Author : KAGEYAMA Yoshiyuki
     
    「光異性化による分子集合体の散逸自己組織化」を世界で初めて実現した。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2014/04 -2016/03 
    Author : TAKEDA SADAMU, KAGEYAMA YOSHIYUKI
     
    In our laboratory, we have found reversible macroscopic motion of supramolecular aggregate was induced by photo-isomerization of doped azo-benzene derivative as a minor component. This phenomenon is similar to biological system, in which motion of molecules is hierarchically and cooperatively developed to induce macroscopic movement. In this research project, we made an attempt to develop “Scanning NMR Microscope” for investigating change at molecular level in supramolecular aggregate at spatial resolution of 100 micrometer by use of micro-coil NMR method. As a complementary approach of this “Scanning NMR Microscope”, enhancement of NMR signal was investigated by use of dynamic nuclear polarization.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2012/04 -2015/03 
    Author : GORO Maruta, KAGEYAMA Yoshiyuki
     
    Changes in temperature of a solid material containing metal ions may cause electron jump between (1) atomic orbitals in the metal ion or (2) the metal ion and carbon atoms or (3) metal ions arranged in the crystal, which in turn causes a drastic change in the magnetic property of the solid. This research has showed that the electron jump can be detected using the accompanying alterations in molecular shape and in ionic size with a commercially available apparatus called NMR spectrometer. With this method, we have succeeded in measuring the frequency of an electron jump, which is difficult to measure by other experimental methods.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2013 -2014 
    Author : KAGEYAMA YOSHIYUKI, TAKEDA Sadamu
     
    As the main component of living cells is water, life phenomena are presented in water. Water surrounds cellular membranes, peptides, and other biomolecules. The surrounding water has different characteristics than general water, and the characteristics are related to the brilliant functions of biomolecules. Therefore, a development of a characteristic water analysis is required to reveal the biological phenomena. Selective analysis of specific water in bulk water is difficult. One potent method is the Overhauser dynamic nuclear polarization nuclear magnetic resonance (DNP-NMR). For our project, we equipped an instrument to measure the water molecules using DNP-NMR. We also used organic synthetic techniques to prepare artificial molecular assemblies, and measured the water surrounding the assemblies. In perspective, it is required to improve the accuracy of quantitative discussions for rheology of water by merging the theoretical approach with further measurements.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2011 -2012 
    Author : KAGEYAMA Yoshiyuki
     
    The aim of the project is to control the motion of sub-millimeter size myelin self-assembly (myelin tube) composed of oleic acid and functional organic molecules. Amphiphilic azobenzene containing myelin tubes showed straighten and coiling motion by photoirradiation with ultraviolet light and visible light, respectively. In the photoinduced macroscopic dynamics, it became clear that both of structural change of azobenzene molecule by photoisomerization and acid dissociation behavior of oleic acids byazobenzene-isomerization take an important role.
  • オレイン酸を主成分とする螺旋状超分子の形成挙動に対する添加分子の役割の解明
    日本科学協会：笹川科学研究助成(学術)
    Date (from‐to) : 2010/04 -2011/03 
    Author : 景山 義之
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2007 -2009 
    Author : 青木 伸, 北村 正典, 景山 義之
     
    アポトーシスは、固体の生命を維持するために、遺伝子によって制御された細胞死であり、不要になった細胞や有害な細胞を除去する現象である。アポトーシスの誘起機構の一つとして、ミトコンドリアから遊離したカスパーゼ-9がカスパーゼ-3を活性化する経路がある。しかし、がん細胞は、カスパーゼ-9と複合体を生成するタンパクであるXIAPが、カスパーゼ-9と複合体を生成して不活性化する防御機構をもっている。 そこで本研究では、XIAPを認識するペプチドと金属イオンキレート部をもつ化合物(L-ペプチド)を設計、合成する。がん細胞内でbpy-ペプチドが金属イオンによって自己集積してM(L-ペプチド)を生成し、M(L-ペプチド)-XIAP複合体の生成によってカスパーゼ-9を遊離してアポトーシス誘導を行う。 平成21年度は、平成20年度に合成した2,2'-bipyridyl(bpy)基を有するジペプチド体とモノペプチド体の合成に加えて、リンカーとしてbis(picolyl)amino(BPA)基とN,N'-bis(picolyl)ethylenediamine(BISPICEN)基を有する化合物の合成に成功した。これらのXIAP結合能を蛍光偏光法によって測定したところ、いずれの化合物もnMオーダーのIC_<50>値を有することが明らかになった。亜鉛イオンの効果を検討したところ、BPAリンカーを有する化合物において、亜鉛イオンの効果が認められた。亜鉛イオン存在下、非存在下での差は決して大きくはないが(Zn^<2+>非存在下で44nM、Zn^<2+>存在下で27nM)、さらに強いZn^<2+>キレーターを加えると、IC_<50>値がZn^<2+>非存在下の値に近づいたことなどから、Zn^<2+>との錯体生成がXIAPへの結合を増大させたことが示唆された。また、弱いながらもがん細胞に対するアポトーシス誘導も観測された。現在、これらの成果を発表するべく、論文投稿の準備中である。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2006 -2009 
    Author : AOKI Shin, YAMADA Yasuyuki, KITAMURA Masanori
     
    (+)-ビオチン(Btn)は、アビジン(Avn)と非常に安定な複合体を生成する。その解離定数Kdは約1fMであり、通常の抗原-抗体複合体の100万倍も強く、実質上不可逆である。本研究は、申請者らが発見したCaged化合物(8-quinolinyl sulfonates, QS)の光分解反応を利用することにより、光分解性Btn標識試薬の開発を目的とした。中性pH、常温という温和な条件下、光照射によってリガンド-レセプター複合体をintactな状態で回収し、特異的レセプターの同定、構造解析を行うための全く新しいBtn-Avnシステムを提供する。 脳内神経伝達分子であるドーパミンを、光分解性QSリンカーを介してビオチンに結合した化合物(Biotin-Dopamine-HQ)の合成を行った。Biotin-Dopamine-HQと抗ドーパミン抗体(IgG)、および二次抗体(抗IgG抗体)を用いてELISAを行い、Avn、Biotin-Dopamine-HQ、IgG、抗IgG抗体の四者の複合体が生成することを確認した。さらにAvn、Biotin-Dopamine-HQ、IgGの三元複合体に光を照射し、リンカーが分解できることを確認し、分解後のDopamine-IgGの、Western blottingによる検出に成功した。以上の結果は、すでに論文発表された(Bioorg. Med. Chem. 2009, 17, 3405-3413)。 また、上記の化合物もよりも長いリンカーを有するビオチン標識リンカーの合成にも成功した。同様にELISA、Western blottingを行ったところ、リンカーの延長は抗体との複合体形成、光分解にDopamine-抗体複合体の回収率に大きな変化はなかった。本結果についても、論文投稿の準備中である。さらに、本技術の基盤となるQS基質の光分解反応の反応機構についても詳細に検討し、論文投稿済みである。 このように、本研究ではQS基質の新規光分解反応の発見、反応機構の解明を行い、それを光分解性Btn標識リンカーにまで応用した。現在、光分解性リポソームなどにも展開可能であり、今後が期待される。

Media Coverage


Copyright © MEDIA FUSION Co.,Ltd. All rights reserved.