研究者データベース
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研究者情報
マスター
アカウント（マスター）
氏名
宇治 利樹(ウジ トシキ), ウジ トシキ
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所属（マスター）
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syllabus
- 2021, 育種生物学特論Ⅱ, Advanced Aquaculture Genetics and Genomics Ⅱ, 修士課程, 水産科学院
- 2021, 水圏生物学実験, Laboratory Works on Aquatic Biology, 学士課程, 水産学部, 受精、細胞分裂、胚発生、形態形成、ストレス応答、性決定、分子生物学、組織学、海藻、魚類
- 2021, 教科教育法（理科II）, Teaching Method of School Subjects(Science Ⅱ), 学士課程, 教育学部
- 2021, 海藻学, Applied Biology of Seaweeds, 学士課程, 水産学部, 海藻、褐藻、紅藻、緑藻、生活史、生殖、形態形成、胞子体、配偶体、多様性、栽培、食文化
- 2021, 水産資源各論, Introduction of Fisheries Biology, 学士課程, 水産学部, 水産生物，分布，生活史，生態，漁獲方法，資源利用
- 2021, 水族遺伝育種学, Aquatic Breeding Genetics, 学士課程, 水産学部, 核酸、遺伝子、染色体、ゲノム、遺伝的多様性、遺伝的集団構造、量的形質、遺伝率、染色体地図、遺伝子組換え、ゲノム編集、遺伝資源、分子育種
- 2021, 基礎生命科学実験, Basic Laboratory Works on Life Science, 学士課程, 水産学部, 組織 器官 解剖 切片 基本操作 緩衝液 吸光度分析
- 2021, 発生・組織学, Embryology and Histology, 学士課程, 水産学部, 器官、組織、細胞、生殖、発生
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宇治名
利樹ID各種
201301094250782816
業績リスト
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経歴
- 2015年10月 - 現在 北海道大学 大学院水産科学院 助教
- 2015年04月 - 2015年09月 熊本大学 沿岸域環境科学教育研究センター 日本学術振興会特別研究員-PD
- 2013年08月 - 2015年03月 北海道大学 大学院水産科学研究院 特任助教
- 2013年06月 - 2013年07月 北海道大学 大学院水産科学研究院 学術研究員
- 2012年06月 - 2013年05月 フランス国立科学センター ロスコフ生物学研究所 研究員 （上原記念生命科学財団海外留学助成ポストドクトラルフェローシップ）
- 2012年04月 - 2012年05月 北海道大学 大学院水産科学研究院 研究員
学歴
受賞
論文
- Toshiki Uji, Takuya Kandori, Hiroyuki MizutaFrontiers in Plant Science 15 2024年08月06日Saccharina japonica, a significant brown macroalga in the Pacific Ocean, serves as a food source and industrial material. In aquaculture, collecting mature sporophytes for seedling production is essential but challenging due to environmental changes. In this study, transcriptomic analysis of vegetative and sorus tissues was done to identify differentially expressed genes (DEGs) and enhance our understanding of sorus formation regulation in S. japonica. KEGG pathway and Gene Otology (GO) analysis revealed that upregulated DEGs were involved in folate biosynthesis, riboflavin metabolism, and amino acid biosynthesis. In addition, the upregulation of genes associated with cell wall remodeling, such as mannuronan C-5-epimerases, vanadium-dependent haloperoxidases, and NADPH oxidase, was observed in sorus parts. Meanwhile, downregulated DEGs in sorus portions included genes related to chloroplast function. These findings will help us understand the regulatory mechanisms behind sorus formation in S. japonica and extracellular matrix remodeling in brown algae.
- Kenshiro ABE, Inori ARIGA, Toshiki UJI, Hiroyuki MIZUTACahiers de Biologie Marine 65 73 - 85 2024年01月 [査読有り]
- Shinnosuke Ueda, Hiroyuki Mizuta, Toshiki UjiMolecular biotechnology 2022年09月13日Epigenetic regulation by histone modification can activate or repress transcription through changes in chromatin dynamics and regulates development and the response to environmental signals in both animals and plants. Chromatin immunoprecipitation (ChIP) is an indispensable tool to identify histones with specific post-translational modifications. The lack of a ChIP technique for macroalgae has hindered understanding of the role of histone modification in the expression of genes in this organism. In this study, a ChIP method with several modifications, based on existing protocols for plant cells, has been developed for the red macroalga, Neopyropia yezoensis, that consists of a heterogeneous alternation of macroscopic leaf-like gametophytes and microscopic filamentous sporophytes. ChIP method coupled with qPCR enables the identification of a histone mark in generation-specific genes from N. yezoensis. The results indicate that acetylation of histone H3 at lysine 9 in the 5' flanking and coding regions from generation-specific genes was maintained at relatively high levels, even in generation-repressed gene expression. The use of this ChIP method will contribute significantly to identify the epigenetic regulatory mechanisms through histone modifications that control a variety of biological processes in red macroalgae.
- Toshiki Uji, Hiroyuki MizutaJOURNAL OF APPLIED PHYCOLOGY 34 3 1527 - 1536 2022年06月Heat stress disrupts algal growth, development, and physiological processes, such as photosynthesis, and eventually decreases seaweed productivity. Previous studies of crop plants have revealed that exogenous application of phytohormones prior or parallel to stress can alleviate the negative effects of abiotic stressors, including heat stress. However, there is limited information on phytohormone-induced tolerance to abiotic stressors in seaweed. In the present study, the application of the major plant hormones abscisic acid and salicylic acid failed to mitigate the negative effects of heat stress on the marine red alga Neopyropia yezoensis, whereas 1-aminocyclopropane-1-carboxylic acid (ACC), the direct precursor of the plant hormone ethylene, regulates thermotolerance. In addition, the ACC analogs 1-aminocyclobutane-1-carboxylic acid and alpha-aminoisobutyric acid enhanced tolerance to heat stress. ACC increased the expression of genes involved in antioxidant defense systems to protect photosynthesis and respiration. These results suggest ACC acts as a phytohormone to mitigate the impact on heat stress independent of ethylene in N. yezoensis.
- Toshiki Uji, Takuya Kandori, Shiho Konishi, Hiroyuki MizutaBMC plant biology 22 1 181 - 181 2022年04月08日BACKGROUND: 1-aminocyclopropane 1-carboxylic acid (ACC) is the immediate precursor of the plant hormone ethylene. However, recent studies have suggested that ACC also acts as a signaling molecule to regulate development and growth independently from ethylene biosynthesis. In red algae, ACC stimulates the switch from a vegetative to a sexual reproductive phase. However, despite evidence that ACC signaling in plants and algae is widespread, the mechanistic basis of the ACC signaling pathway remains unknown. RESULTS: We demonstrate that exogenous ACC increased the activity of phospholipase D (PLD) and induced the accumulation of PLD transcripts in the marine red alga Neopyropia yezoensis. The product of PLD, the lipid second messenger phosphatidic acid (PA), also increased in response to ACC. Furthermore, the pharmacological inhibition of PLD by 1-butanol blocked ACC-induced spermatangia and carpospore production, but the inactive isomer t-butanol did not. In addition, 1-butanol prevented ACC-induced growth inhibition and inhibited transcript accumulation of genes upregulated by ACC, including extracellular matrix (ECM)-related genes, and alleviated the transcriptional decrease of genes downregulated by ACC, including photosynthesis-related genes. CONCLUSIONS: These results indicate that PLD is a positive regulator of sexual cell differentiation and a negative regulator of growth. This study demonstrates that PLD and its product, PA, are components of ACC signaling during sexual reproduction in N. yezoensis.
- Sayaka Kominami, Hiroyuki Mizuta, Toshiki UjiMarine biotechnology (New York, N.Y.) 24 2 393 - 407 2022年04月Many organisms are subjected to a daily cycle of light and darkness, which significantly influences metabolic and physiological processes. In the present study, Neopyropia yezoensis, one of the major cultivated seaweeds used in "nori," was harvested in the morning and evening during light/dark treatments to investigate daily changes in gene expression using RNA-sequencing. A high abundance of transcripts in the morning includes the genes associated with carbon-nitrogen assimilations, polyunsaturated fatty acid, and starch synthesis. In contrast, the upregulation of a subset of the genes associated with the pentose phosphate pathway, cell cycle, and DNA replication at evening is necessary for the tight control of light-sensitive processes, such as DNA replication. Additionally, a high abundance of transcripts at dusk encoding asparaginase and glutamate dehydrogenase imply that regulation of asparagine catabolism and tricarboxylic acid cycle possibly contributes to supply nitrogen and carbon, respectively, for growth during the dark. In addition, genes encoding cryptochrome/photolyase family and histone modification proteins were identified as potential key players for regulating diurnal rhythmic genes.
- Toshiki Uji, Hiroyuki MizutaFrontiers in plant science 13 1019334 - 1019334 2022年Seaweeds or macroalgae are important primary producers that serve as a habitat for functioning ecosystems. A sustainable production of macroalgae has been maintained by a diverse range of life cycles. Reproduction is the most dynamic change to occur during its life cycle, and it is a key developmental event to ensure the species' survival. There is gradually accumulating evidence that plant hormones, such as abscisic acid and auxin, have a role on the sporogenesis of brown alga (Saccharina japonica). Recent studies reported that 1-aminocylopropane-1-carboxylic acid, an ethylene precursor, regulates sexual reproduction in red alga (Neopyropia yezoensis) independently from ethylene. In addition, these macroalgae have an enhanced tolerance against abiotic and biotic stresses during reproduction to protect their gametes and spores. Herein, we reviewed the current understanding on the regulatory mechanisms of red and brown algae on their transition from vegetative to reproductive phase.
- Hiroyuki Mizuta, Toshiki Uji, Hajime YasuiALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 58 2021年10月 [査読有り]
The process of biogenic silicon (Si) deposition in sporophytes of kelp (Saccharina japonica) was investigated by culturing sporophyte disks in the medium with and without hydrogen peroxide (H2O2). Culture with H2O2 remarkably promoted silicate-Si uptake by the disks. Silicate-Si uptake was completely inhibited by the treatment with potassium bromide (KBr), a competitive inhibitor of iodoperoxidase (IPO), even in the medium with H2O2. In addition, disk culture with phenol red in the medium with H2O2 resulted in the formation of bromophenol blue, indicating the presence of extracellular IPO. However, KBr treatment inhibited the formation of bromophenol blue in the medium with H2O2. These results showed that silicate-Si uptake is activated by extracellular IPO in the presence of radical oxygen species (ROS), such as H2O2. Interestingly, 1 mM salicylic acid also promoted silicate-Si uptake, even in the medium without H2O2. Furthermore, the allocation of silicate-Si to the soluble and cell wall-bound phlorotannin fractions using rhodamine 123 (R123), a tracer of biogenic Si, was estimated. Tracer fluorescence was detected in not only the soluble but also the cell wall-bound phlorotannin fraction. R123 fluorescence was histologically detected in the apoplasts of the epidermal and outer cortical layers of the disk, overlapping the histochemical distribution of IPO activity. These results suggested that silicate-Si uptake is activated by an oxidative burst induced by various stresses, and Si deposition is catalyzed by extracellular IPO in the presence of both ROS and phlorotannin in the extracellular matrix of sporophytes. Based on these findings, the hypothetical mechanisms of the extracellular silicate-Si uptake for defense responses in kelp were discussed. - Toshiki Uji, Hiroyuki MizutaAQUACULTURE RESEARCH 52 12 6814 - 6817 2021年08月 [査読有り]
- Harune Endo, Hiroyuki Mizuta, Toshiki UjiJournal of Applied Phycology 33 2 1081 - 1087 2021年04月01日1-aminocyclopropane-1-carboxylic acid (ACC), a precursor for ethylene, stimulates the switch from a vegetative to a sexual reproductive phase in the marine red alga Pyropia species. This study explored the effects of ethylene biosynthesis inhibitors on the sexual reproduction of gametophytes of the red alga Pyropia yezoensis to gain a functional understanding of the role of ACC as a plant hormone in red algae. Here we show that two inhibitors of ACC synthesis in higher plants, 2-aminoethoxyvinyl glycine (AVG) and aminooxyacetic acid (AOA), had no effect on the growth and gametogenesis of P. yezoensis. In contrast, exogenous application of α-aminoisobutyric acid (AIB), a structural analog of ACC that blocks the conversion of ACC to ethylene in higher plants, induced the formation of spermatangia and carpospores in a similar manner as ACC, without endogenous ACC accumulation. The treatment of AIB failed to inhibit ethylene production in the gametophytes. The present results suggest that AIB mimics the effect of ACC to induce the sexual reproduction and support our previous study that ACC has a role in the regulation of the sexual reproduction independent from ethylene.
- Yuya Kumagai, Keigo Toji, Satoshi Katsukura, Rie Morikawa, Toshiki Uji, Hajime Yasui, Takeshi Shimizu, Hideki KishimuraMarine drugs 19 4 2021年04月01日More than 7000 red algae species have been classified. Although most of them are underused, they are a protein-rich marine resource. The hydrolysates of red algal proteins are good candidates for the inhibition of the angiotensin-I-converting enzyme (ACE). The ACE is one of the key factors for cardiovascular disease, and the inhibition of ACE activity is related to the prevention of high blood pressure. To better understand the relationship between the hydrolysates of red algal proteins and the inhibition of ACE activity, we attempted to identify novel ACE inhibitory peptides from Pyropia pseudolinearis. We prepared water soluble proteins (WSP) containing phycoerythrin, phycocyanin, allophycocyanin, and ribulose 1,5-bisphosphate carboxylase/oxygenase. In vitro analysis showed that the thermolysin hydrolysate of the WSP had high ACE inhibitory activity compared to that of WSP. We then identified 42 peptides in the hydrolysate by high-performance liquid chromatography and mass spectrometry. Among 42 peptides, 23 peptides were found in chloroplast proteins. We then synthesized the uncharacterized peptides ARY, YLR, and LRM and measured the ACE inhibitory activity. LRM showed a low IC50 value (0.15 μmol) compared to ARY and YLR (1.3 and 5.8 μmol). In silico analysis revealed that the LRM sequence was conserved in cpcA from Bangiales and Florideophyceae, indicating that the novel ACE inhibitory peptide LRM was highly conserved in red algae.
- Toshiki Uji, Harune Endo, Hiroyuki MizutaFrontiers in plant science 11 60 - 60 2020年 [査読有り][通常論文]
The transition from the vegetative to sexually reproductive phase is the most dynamic change to occur during a plant's life cycle. In the present study, we showed that the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) induces sexual reproduction in the marine red alga Pyropia yezoensis independently from ethylene. Exogenous application of ACC, which contains a three membered carbocyclic ring, promoted the formation of spermatia and carporspores in gametophytes, whereas ethephon, an ethylene-releasing compound, did not stimulate sexual reproduction. In addition, an ACC analog, 1-aminocyclobutane-1-carboxylic acid (ACBC), which contains a four membered carbocyclic ring, promoted sexual reproduction and enhanced tolerance to oxidative stress in the same manner as ACC, but 1-aminocyclopentane-1-carboxylic acid (cycloleucine; which contains a cyclopentane ring) did not. The application of ACC increased the generation of reactive oxygen species (ROS) and induced the expression of PyRboh gene encoding NADPH oxidase. ACC also stimulated the synthesis of ascorbate (AsA) by inducing transcripts of PyGalLDH, which encodes galactono-1,4-lactone dehydrogenase, the catalyst for the final enzymatic step of the AsA biosynthetic pathway. Conversely, ACC caused a decrease in the synthesis of glutathione (GSH) by repressing transcripts of PyGCL, which encodes glutamate cysteine ligase, the catalyst for the rate-limiting step in the formation of GSH. These results suggest a possible role played by ACC as a signaling molecule independent from ethylene in the regulation of sexual reproduction through alterations to the redox state in P. yezoensis. - Ryo Yanagisawa, Naoki Sekine, Hiroyuki Mizuta, Toshiki UjiJOURNAL OF APPLIED PHYCOLOGY 31 5 3317 - 3329 2019年10月 [査読有り][通常論文]
The marine red algal genus Pyropia, class Bangiophyceae, includes dioecious and monoecious species; however, the molecular mechanisms underlying control of their sexual reproduction are still poorly understood. In the present study, we demonstrated that application of the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), promoted the formation of spermatangia and parthenosporangia in male and female gametophytes, respectively, of the dioecious species Pyropia pseudolinearis. In addition, we determined expression profiles of ACC-responsive genes in the gametophytes during sexual reproduction using RNA-Seq and quantitative real-time PCR (qRT-PCR). Genes involved in the regulation of cell division and cell wall organization, such as high-mobility group (PpHMG) and glycosyltransferase family (PpGT14), were found to be upregulated in male and female gametophytes treated with ACC. In addition, the relatively rapid ACC-response of the vesicular-trafficking-related genes, flotillin (PpFLOT), charged multivesicular body protein 5 (PpCHMP5), and peptidase family S8 (PpS8) was shown to occur during male and female sexual reproduction. Expression levels of these six genes in the monoecious species P. yezoensis, which are homologs to ACC-responsive genes in P. pseudolinearis, also increased in gametophytes treated with ACC. These findings could provide new insights into the ACC-regulation of the sexual life cycle in Pyropia species. - Watanabe Kei, Kishimoto Takahiro, Kumagai Yuya, Shimizu Takeshi, Uji Toshiki, Yasui Hajime, Kishimura HidekiMITOCHONDRIAL DNA PART B-RESOURCES 4 2 2543 - 2544 2019年07月03日 [査読有り][通常論文]
- Toshiki Uji, Yohei Gondaira, Satoru Fukuda, Hiroyuki Mizuta, Naotsune SagaCell stress & chaperones 24 1 223 - 233 2019年01月 [査読有り][通常論文]
Small heat shock proteins (sHSPs) are found in all three domains of life (Bacteria, Archaea, and Eukarya) and play a critical role in protecting organisms from a range of environmental stresses. However, little is known about their physiological functions in red algae. Therefore, we characterized the sHSPs (PysHSPs) in the red macroalga Pyropia yezoensis, which inhabits the upper intertidal zone where it experiences fluctuating stressful environmental conditions on a daily and seasonal basis, and examined their expression profiles at different developmental stages and under varying environmental conditions. We identified five PysHSPs (PysHSP18.8, 19.1, 19.2, 19.5, and 25.8). Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that expression of the genes PysHSP18.8, PysHSP19.5, and PysHSP25.8 was repressed at all the developmental stages under normal conditions, whereas PysHSP19.1 and PysHSP19.2 were overexpressed in mature gametophytes and sporophytes. Exposure of the gametophytes to high temperature, oxidative stress, or copper significantly increased the mRNA transcript levels of all the five genes, while exogenous application of the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) significantly increased the expression levels of PysHSP19.2, PysHSP19.5, and PysHSP25.8. These findings will help to further our understanding of the role of PysHSP genes and provide clues about how Pyropia species can adapt to the stressful conditions encountered in the upper intertidal zone during their life cycle. - Kazuki Shimizu, Toshiki Uji, Hajime Yasui, Hiroyuki MizutaJournal of Applied Phycology 30 2 1371 - 1379 2018年04月01日 [査読有り][通常論文]
In this study, the oligoguluronate elicitor-induced oxidative burst (OB) was monitored continuously in young and mature Saccharina japonica sporophytes based on luminol chemiluminescence using a photon counter. The iodoperoxidase (IPO) activity, abscisic acid (ABA), and polyphenol contents were also compared in the different growth stages. The elicitor-induced OB occurred within 1 min and reached its maximum in 15–20 min after treatment in all growth stages. The active elicitor-induced OB was stronger in the young sporophytes than the older sporophytes. The IPO activity in the different growth stages also exhibited a similar pattern to the elicitor-induced OB. These results suggest that the elicitor-induced OB and the subsequent high haloperoxidase activity comprise a major defence mechanism in young sporophytes. By contrast, ABA accumulated with the growth of the sporophytes. Interestingly, ABA treatment suppressed the elicitor-induced OB during growth and enhanced the elicitor-independent IPO activity even in the young sporophytes. In addition, the polyphenol content was higher in the older sporophytes than the younger sporophytes. These observations show that dramatic changes occur in the characteristic defences against biotic stresses as the sporophyte grows, as well as suggesting that ABA is closely linked with these changes. Moreover, the IPO activity recovered slightly in the sorus, which is the reproductive tissue, thereby suggesting that a higher ABA content increases the defence activity and the success of reproduction. - Olivier Godfroy, Toshiki Uji, Chikako Nagasato, Agnieszka P. Lipinska, Delphine Scornet, Akira F. Peters, Komlan Avia, Sebastien Colin, Laure Mignerot, Taizo Motomura, J. Mark Cock, Susana M. CoelhoPlant Cell 29 12 3102 - 3122 2017年12月01日 [査読有り][通常論文]
Brown algae are one of the most developmentally complex groups within the eukaryotes. As in many land plants and animals, their main body axis is established early in development, when the initial cell gives rise to two daughter cells that have apical and basal identities, equivalent to shoot and root identities in land plants, respectively. We show here that mutations in the Ectocarpus DISTAG (DIS) gene lead to loss of basal structures during both the gametophyte and the sporophyte generations. Several abnormalities were observed in the germinating initial cell in dis mutants, including increased cell size, disorganization of the Golgi apparatus, disruption of the microtubule network, and aberrant positioning of the nucleus. DIS encodes a TBCCd1 protein, which has a role in internal cell organization in animals, Chlamydomonas reinhardtii, and trypanosomes. Our study highlights the key role of subcellular events within the germinating initial cell in the determination of apical/basal cell identities in a brown alga and emphasizes the remarkable functional conservation of TBCCd1 in regulating internal cell organization across extremely distant eukaryotic groups. - Toshiki Uji, Ryuya Matsuda, Katsuaki Takechi, Hiroyoshi Takano, Hiroyuki Mizuta, Susumu TakioJOURNAL OF APPLIED PHYCOLOGY 28 6 3501 - 3509 2016年12月 [査読有り][通常論文]
Plant growth regulators (PGRs) play a pivotal role in vascular plants, regulating growth, development, and stress responses; however, the role of PGRs in algae remains largely unexplored. Here, the role of ethylene, a simple plant growth regulator, was demonstrated in sexual reproduction of the marine red alga Pyropia yezoensis. Application of the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) promoted the formation of spermatia and zygotospores in the gametophytes as well as ethylene production, whereas the growth rate was repressed in comparison to gametophytes not treated with ACC. In addition, gametophytes treated with ACC and mature gametophytes showed enhanced tolerance to oxidative stress. Gene expression profiles revealed upregulation of genes involved in cell division and stress response in gametophytes treated with ACC and in mature gametophytes. These results indicate that ethylene plays an important role in the regulation of gamete formation and protection against stress-induced damage during the sexual reproductive stage. Considered together, these findings demonstrate that ethylene is involved in regulating the switching from a vegetative to a sexual reproductive phase in P. yezoensis. - Toshiki Uji, Daisuke Nanaumi, Chikara Kawagoe, Naotsune Saga, Kazuo MiyashitaJOURNAL OF APPLIED PHYCOLOGY 28 4 2435 - 2443 2016年08月 [査読有り][通常論文]
A high frequency of callus induction and propagation from leaf explants of the brown alga Sargassum horneri was achieved within 2 months of culture when grown in medium supplemented with 5 mu M uniconazole, which is a triazole- type inhibitor of cytochrome P450 enzymes. Adventitious buds were efficiently formed from the pigmented callus after transfer to medium without uniconazole, indicating that treatment with uniconazole was more beneficial for regeneration of the alga. Favorable culture conditions for induction of adventitious buds and calli included temperatures of 15 to 25 degrees C and light levels of 20 to 200 mu mol photons m(-2) s(-1). Blue light promoted the production of adventitious buds and calli. The frequency of formation of adventitious buds and calli in explants from thalli with one leaf was more than 90 %, while it was 10 % only when explants were sourced from thalli with 9 to 11 leaves. These findings will be useful for clonal propagation and storage of seed materials for mariculture of Sargassum species. - Hirotoshi Endo, Megumi Yoshida, Toshiki Uji, Naotsune Saga, Koji Inoue, Hiromichi NagasawaSCIENTIFIC REPORTS 6 2016年03月 [査読有り][通常論文]
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum. - Akira Inoue, Chieco Mashino, Toshiki Uji, Naotsune Saga, Koji Mikami, Takao OjimaCurrent biotechnology 4 3 240 - 248 2015年08月 [査読有り][通常論文]
BACKGROUND: Alginate lyases belonging to polysaccharide lyase family-7 (PL-7) are the most well studied on their structures and functions among whole alginate lyases. However, all characterized PL-7 alginate lyases are from prokaryotic bacteria cells. Here we report the first identification of eukaryotic PL-7 alginate lyase from marine red alga Pyropia yezoensis. METHODS: The cDNA encoding an alginate lyase PyAly was cloned and was used for the construction of recombinant PyAly (rPyAly) expression system in Escherichia coli. Purified rPyAly was assayed to identify its enzymatic properties. Its expression pattern in P. yessoensis was also investigated. RESULTS: PyAly is likely a secreted protein consisting of an N-terminal signal peptide of 25 residues and a catalytic domain of 216 residues. The amino-acid sequence of the catalytic domain showed 19-29% identities to those of bacterial characterized alginate lyases classified into family PL-7. Recombinant PyAly protein, rPyAly, which was produced with E. coli BL21(DE3) by cold-inducible expression system, drastically decreased the viscosity of alginate solution in the early stage of reaction. The most preferable substrate for rPyAly was the poly(M) of alginate with an optimal temperature and pH at 35oC and 8.0, respectively. After reaction, unsaturated tri- and tetra-saccharides were produced from poly(M) as major end products. These enzymatic properties indicated that PyAly is an endolytic alginate lyase belonging to PL-7. Moreover, we found that the PyAly gene is split into 4 exons with 3 introns. PyAly was also specifically expressed in the gametophytic haplopid stage. CONCLUSION: This study demonstrates that PyAly in marine red alga P. yezoensis is a novel PL-7 alginate lyase with an endolytic manner. PyAly is a gametophyte-specifically expressed protein and its structural gene is composed of four exons and three introns. Thus, PyAly is the first enzymatically characterized eukaryotic PL-7 alginate lyase. - 有用海藻における育種研究の現状と将来展望.宇治 利樹水産育種, 44:pp.41-48 2015年 [査読無し]
- J. Mark Cock, Alok Arun, Delphine Scornet, Akira F. Peters, Olivier Godfroy, Alexandre Cormier, Nicolas Macaisne, Toshiki Uji, Sophia Ahmed, Susana M. CoelhoEUROPEAN JOURNAL OF PHYCOLOGY 50 94 - 94 2015年 [査読有り][通常論文]
- Ryo Hirata, Toshiki Uji, Satoru Fukuda, Hiroyuki Mizuta, Asao Fujiyama, Satoshi Tabata, Naotsune SagaJOURNAL OF APPLIED PHYCOLOGY 26 4 1863 - 1868 2014年08月 [査読有り][通常論文]
Genetic transformation systems using reporter genes in whole plants have a wide variety of applications for molecular biological study including the visualization of expression patterns of particular genes and intracellular biological phenomena as well as the identification of novel genes. In this study, we assessed co-expression of each three codon-optimized reporter genes and a selectable marker in the nuclear transformation system of whole Pyropia yezoensis, a red marine alga. With the use of an endogenous promoter, both the codon-optimized hygromycin resistance gene and -glucuronidase gene (PyGUS) were co-expressed in P. yezoensis cells. A high level of GUS activity was observed in 60 % of the individuals in hygromycin-resistant lines. A histochemical GUS assay revealed that the PyGUS reporter gene was stably introduced and expressed throughout the algae's life cycle. In addition, two live cell reporters, humanized cyan fluorescent protein from Anemonia majano and luciferase from Gaussia princeps, were successfully expressed in whole P. yezoensis. The development of this transformation system involving three types of reporter genes provides opportunities for monitoring temporal changes in gene expression and for genetic screening in red marine algae. - Toshiki Uji, Ryo Hirata, Satoru Fukuda, Hiroyuki Mizuta, Naotsune SagaMarine biotechnology (New York, N.Y.) 16 3 251 - 5 2014年06月 [査読有り][通常論文]
Marine macroalgae play an important role in marine coastal ecosystems and are widely used as sea vegetation foodstuffs and for industrial purposes. Therefore, there have been increased demands for useful species and varieties of these macroalgae. However, genetic transformation in macroalgae has not yet been established. We have developed a dominant selection marker for stable nuclear transformation in the red macroalga Pyropia yezoensis. We engineered the coding region of the aminoglycoside phosphotransferase gene aph7″ from Streptomyces hygroscopicus to adapt codon usage of the nuclear genes of P. yezoensis. We designated this codon-optimized aph7″ gene as PyAph7. After bombarding P. yezoensis cells with plasmids containing PyAph7 under the control of their endogenous promoter, 1.9 thalli (or individuals) of hygromycin-resistant strains were isolated from a 10-mm square piece of the bombarded thallus. These transformants were stably maintained throughout the asexual life cycle. Stable expression of PyAph7was verified using Southern blot analysis and genomic PCR and RT-PCR analyses. PyAph7 proved to be a new versatile tool for stable nuclear transformation in P. yezoensis. - Toshiki Uji, Rei Sato, Hiroyuki Mizuta, Naotsune SagaJOURNAL OF APPLIED PHYCOLOGY 25 6 1887 - 1893 2013年12月 [査読有り][通常論文]
Multiprotein bridging factor 1 (MBF1) is a highly conserved transcriptional co-activator involved in the regulation of diverse processes, such as environmental stress responses. We recently identified a novel MBF1 gene, PyMBF1, from the marine red alga Pyropia yezoensis. In this study, quantitative real-time PCR analysis revealed that PyMBF1 transcripts were upregulated in P. yezoensis cells during exposure to oxidative and heat stresses. We also examined heat signaling in P. yezoensis cells by monitoring the accumulation of PyMBF1 transcripts. Heat activation of PyMBF1 was inhibited by the membrane rigidifier dimethylsulfoxide, whereas it was induced without heat stress by the membrane fluidizer benzyl alcohol (BA). Induction of PyMBF1 transcripts by heat and BA was inhibited by 1-butanol, an inhibitor of phospholipase D (PLD). The results suggest that the heat activation of PyMBF1 requires membrane fluidization and activation of PLD. These findings provide an initial step toward understanding heat signaling in marine red algae. - Toshiki Uji, Hiroyuki Mizuta, Naotsune SagaMARINE BIOTECHNOLOGY 15 2 188 - 196 2013年04月 [査読有り][通常論文]
The life cycle of plants entails an alternation of generations, the diploid sporophyte and haploid gametophyte stages. There is little information about the characteristics of gene expression during each phase of marine macroalgae. Promoter analysis is a useful method for understanding transcriptional regulation; however, there is no report of promoter analyses in marine macroalgae. In this study, with the aim of elucidating the differences in the transcriptional regulatory mechanisms between the gametophyte and sporophyte stages in the marine red alga Porphyra yezoensis, we isolated the promoter from the sporophyte preferentially expressed gene PyKPA1, which encodes a sodium pump, and analyzed its promoter using a transient gene expression system with a synthetic beta-glucuronidase (PyGUS) reporter. The deletion of -1432 to -768 relative to the transcription start site resulted in decreased GUS activity in sporophytes. In contrast, deletion from -767 to -527 increased GUS activity in gametophytes. Gain-of-function analyses showed that the -1432 to -760 region enhanced the GUS activity of a heterologous promoter in sporophytes, whereas the -767 to -510 region repressed it in gametophytes. Further mutation and gain-of-function analyses of the -767 to -510 region revealed that a 20-bp GC-rich sequence (-633 to -614) is responsible for the gametophyte-specific repressed expression. These results showed that the sporophyte-specific positive regulatory region and gametophyte-specific negative regulatory sequence play a crucial role in the preferential expression of PyKPA1 in P. yezoensis sporophytes. - Toshiki Uji, Ryo Hirata, Koji Mikami, Hiroyuki Mizuta, Naotsune SagaMOLECULAR BIOLOGY REPORTS 39 8 7973 - 7980 2012年08月 [査読有り][通常論文]
Sodium pumps (EC 3.6.3.9, Na+-ATPase), which mediate excretion of Na+ from the cell, play a crucial role in Na+ homeostasis in eukaryotic cells. The objective of this study is to understand the Na+ efflux system in a marine red alga. We identified a novel sodium pump gene, PyKPA2, from the marine red alga Porphyra yezoensis. The amino acid sequence of PyKPA2 shares 65 % identity with PyKPA1, a previously identified P. yezoensis sodium pump. Similar to PyKPA1, PyKPA2 contains conserved sequences for functions such as phosphorylation, ATP binding, and cation binding. Phylogenetic analysis revealed that the two genes cluster with sodium pumps from algae. Reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that PyKPA1 is expressed preferentially in sporophytes, whereas PyKPA2 is expressed specifically in gametophytes. RT-PCR and quantitative real-time PCR analysis revealed that PyKPA1 and PyKPA2 transcripts were upregulated and downregulated, respectively, in gametophytes during exposure to alkali stress. In addition, transcription of both genes in gametophytes was also induced by cold stress. These results suggest that PyKPA1 and PyKPA2 play an important role in alkali and cold stress tolerance. - Koji Mikami, Toshiki Uji, Ryo Hirata, Megumu Takahashi, Naotsune SagaNIPPON SUISAN GAKKAISHI 78 2 271 - 271 2012年03月 [査読有り][通常論文]
- ノリ育種の現状とゲノム情報利用の将来展望三上浩司, 平田遼, 宇治利樹, 髙橋潤, 嵯峨直恆水産育種, 41: pp. 161-168 2012年
- Koji Mikami, Laura Saavedra, Yuji Hiwatashi, Toshiki Uji, Mitsuyasu Hasebe, Marianne SommarinPLANT PHYSIOLOGY 153 3 1004 - 1015 2010年07月 [査読有り][通常論文]
Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization was confirmed with Arabidopsis (Arabidopsis thaliana) AtPIP5K1. Our findings, in which substitution of a conserved dibasic amino acid pair in the activation loop of PpPIPK1 completely prevented plasma membrane targeting and abolished enzymatic activity, demonstrate its critical role in these processes. Placing our results in the context of studies of eukaryotic PIPKs led us to conclude that the function of the dibasic amino acid pair in the activation loop in type I/II PIPKs is plant specific. - Toshiki Uji, Megumu Takahashi, Naotsune Saga, Koji MikamiMARINE BIOTECHNOLOGY 12 2 150 - 159 2010年04月 [査読有り][通常論文]
Transcription factors play a central role in expression of genomic information in all organisms. The objective of our study is to analyze the function of transcription factors in red algae. One way to analyze transcription factors in eukaryotic cells is to study their nuclear localization, as reported for land plants and green algae using fluorescent proteins. There is, however, no report documenting subcellular localization of transcription factors from red algae. In the present study, using the marine red alga Porphyra yezoensis, we confirmed for the first time successful expression of humanized fluorescent proteins (ZsGFP and ZsYFP) from a reef coral Zoanthus sp. and land plant-adapted sGFP(S65T) in gametophytic cells comparable to expression of AmCFP. Following molecular cloning and characterization of transcription factors DP-E2F-like 1 (PyDEL1), transcription elongation factor 1 (PyElf1) and multiprotein bridging factor 1 (PyMBF1), we then demonstrated that ZsGFP and AmCFP can be used to visualize nuclear localization of PyElf1 and PyMBF1. This is the first report to perform visualization of subcellular localization of transcription factors as genome-encoded proteins in red algae. - Megumu Takahashi, Toshiki Uji, Naotsune Saga, Koji MikamiElectronic Journal of Biotechnology 13 2 1 - 4 2010年 [査読有り][通常論文]
Despite the recent progress of transient gene expression systems in a red alga Porphyra yezoensis by particle bombardment, a stable transformation system has yet to establish in any marine red macrophytes. One of the reasons of the difficulty in genetic transformation in red algae is the lack of systems to select and isolate transformed cells from gametophytic blades. Thus, toward the establishment of the stable transformation system in P. yezoensis, we have developed a procedure by which transiently transformed gametophytic cells were prepared from particle bombarded-gametophytic blade as regeneratable protoplasts. Using mixture of marine bacterial enzymes, yield of protoplasts was high as reported elsewhere however, these protoplasts did not develop. In contrast, protoplasts prepared from gametophytes treated with allantoin were normally developed, in which the overexpression of a β-glucuronidase reporter gene had no effect on the regeneration of protoplasts. Therefore, the use of allantoin in protoplast preparation sheds a new light on the realization of an efficient isolation and selection of study transformed cells from gametophytic blades. © 2010 by Pontificia Universidad Católica de Valparaíso - Chile. - Koji Mikami, Toshiki Uji, Lin Li, Megumu Takahashi, Hajime Yasui, Naotsune SagaMARINE BIOTECHNOLOGY 11 5 563 - 569 2009年10月 [査読有り][通常論文]
Phosphoinositides (PIs) play important roles in signal transduction pathways and the regulation of cytoskeleton and membrane functions in eukaryotes. Subcellular localization of individual PI derivative is successfully visualized in yeast, animal, and green plant cells using PI derivative-specific pleckstrin homology (PH) domains fused with a variety of fluorescent proteins; however, expression of fluorescent proteins has not yet been reported in any red algal cells. In the present study, we developed the system to visualize these PIs using human PH domains fused with a humanized cyan fluorescent protein (AmCFP) in the red alga Porphyra yezoensis. Plasma membrane localization of AmCFP fused with the PH domain from phospholipase C delta 1 and Akt1, but not Bruton's tyrosine kinase, was observed in cell wall-free monospores, demonstrating the presence of phosphatidylinositol-4,5-bisphosphate and phosphatidylinositol-3,4-bisphosphate in P. yezoensis cells. This is the first report of the successful expression of fluorescent protein and the monitoring of PI derivatives in red algal cells. Our system, based on transient expression of AmCFP, could be applicable for the analysis of subcellular localization of other proteins in P. yezoensis and other red algal cells. - Yukihiro Kitade, Michiko Nakamura, Toshiki Uji, Satoru Fukuda, Hirotoshi Endo, Naotsune SagaGENE 423 1 79 - 84 2008年10月 [査読有り][通常論文]
The marine red alga Porphyra yezoensis contains an actin gene family consisting of at least four isoforms (PyACT1, 2, 3 and 4). The amino acid identity between isoforms exceeds 83%, and each contains a putative nuclear export signal (NES). We scanned the sequences for amino acids in regions homologous to the intermonomeric interface of actin filaments. Few residues expected to engage in cross-linking were conserved between the four isoforms. The results of the sequence analyses suggest that PyACT2 probably functions in the nucleus as a monomer (G-actin) or in other unconventional forms. In addition, the distribution and position of the introns were different from those in florideophycean actin genes. The expression level of PyACT3 in matured gametophytes was significantly higher than in those in a vegetative state, although the mRNA was detected at similar levels in both apical and basal parts of thalli. The expression levels of PyACT2 and 4, on the other hand, did not change significantly between the matured and vegetative gametophytes. The PyACT3 may serve as a molecular marker for monitoring thallus maturation in this species. (C) 2008 Elsevier B.V. All rights reserved. - Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensisSatoru Fukuda, Koji Mikami, Toshiki Uji, Eun-Jeong Park, Toshiharu Ohba, Kiyozo Asada, Yukihiro Kitade, Hirotoshi Endo, Ikunoshin Kato, Naotsune SagaPLANT SCIENCE 174 3 329 - 339 2008年03月 [査読有り][通常論文]
The marine red alga Porphyra yezoensis has been proposed as a model plant for physiological and genetic studies in seaweeds because of its biological and economical importance. However, the progress of molecular biological studies using gene transfection and genetic transformation systems has been hindered by difficulties in the expression of foreign genes in P. yezoensis cells. To overcome this situation, we developed a transient gene expression system to monitor gene expression in P. yezoensis cells. An artificial beta-glucuronidase (GUS) coding region was synthesized to adapt it to the codon usage of P. yezoensis (PyGUS) and then evaluated for efficiency as a reporter of transient gene expression by particle bombardment. We also demonstrated the importance of using the promoter of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from P. yezoensis for efficient expression of PyGUS, because the cauliflower mosaic virus (CaMV) 35S promoter, which has been successfully used for monitoring gene expression in nuclei and chloroplasts of higher plants, was less active in P. yezoensis cells. Therefore, the lack of knowledge about differences in the regulatory machinery of gene expression between P. yezoensis and terrestrial plants seems to be why experimental systems for monitoring gene expression were previously not developed in P. yezoensis. Establishment of the transient gene expression system in P yezoensis could facilitate biotechnological developments in this organism. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
MISC
- 海苔の原料であるスサビノリにおけるエチレン前駆物質の役割について宇治 利樹 アグリバイオ 2022年10月
- Toshiki Uji, Shinnosuke Ueda, Hiroyuki Mizuta Phycology 2 (1) 45 -59 2022年01月06日 [査読無し]
- 木下康宣, 木村和世, 川越力, 高野智宏, 熊谷祐也, 岸村栄毅, 宇治利樹, 水田浩之 北海道立工業技術センター研究成果発表会要旨集(Web) 2021 2021年
- 樋口淳也, 河合俊郎, 宇治利樹, 今井圭理 北海道大学水産科学研究彙報(Web) 71 (1) 2021年
- 泉ひかり, 宇治利樹, 水田浩之, 嵯峨直恆, 福田覚 日本水産学会大会講演要旨集 2020 2020年
- 松田竜也, 宇治利樹, 武智克彰, 高野博嘉, 瀧尾進 マリンバイオテクノロジー学会大会講演要旨集 17th 114 2015年05月30日 [査読無し][通常論文]
- 宇治利樹, 平田遼, 福田覚, 水田浩之, 嵯峨直恆 日本水産学会大会講演要旨集 2014 2014年
- Toshiki Uji, Ryota Monma, Hiroyuki Mizuta, Naotsune Saga FISHERIES SCIENCE 78 (5) 985 -991 2012年09月 [査読無し][通常論文]
Na+/H+ antiporters are known to play a crucial role in pH and Na+ homeostasis. In the present study, we characterized the molecular structures and expression patterns of two Na+/H+ antiporters from the marine red alga Porphyra yezoensis (designated PySOS1 and PyNhaD). The full-length cDNAs of PySOS1 and PyNhaD were 5122 and 1804 bp, and contained open reading frames (ORFs) of 4773 and 1275 bp, respectively. The deduced amino acid sequences showed high similarity to SOS1 and NhaD from the higher plant Arabidopsis thaliana. PySOS1 and PyNhaD contained conserved sequences found in the cation-proton antiporter. Quantitative real-time PCR analysis revealed that both antiporter genes were expressed in both the gametophyte and sporophyte of P. yezoensis. In addition, mRNA expression of PySOS1 and PyNhaD was simultaneously upregulated by light irradiation, suggesting that coordinated activity between the two is important in pH and Na+ homeostasis under light conditions. Moreover, the expression levels of both genes were partially reduced by the photosynthetic inhibitors DCMU and DBMIB, suggesting that upregulation is linked to photosynthesis-related metabolism. These findings provide an initial step towards understanding Na+/H+ antiporters in marine red algae. - Su Hyun Son, Joon-Woo Ahn, Toshiki Uji, Dong-Woog Choi, Eun-Jeong Park, Mi Sook Hwang, Jang Ryol Liu, Dongsu Choi, Koji Mikami, Won-Joong Jeong JOURNAL OF APPLIED PHYCOLOGY 24 (1) 79 -87 2012年01月 [査読無し][通常論文]
Porphyra is a commercially valuable source of food and drugs and an important model organism for algal research. However, genetic research on Porphyra tenera has been limited by a lack of a heterologous gene expression system. In this study, we isolated native promoter PtHSP70 for the efficient expression of foreign genes in this organism. This promoter lies approximately 1 kb upstream of the heat shock protein 70 coding sequence and was isolated using adapter ligation-mediated genomic polymerase chain reaction. Promoter activity was evaluated using the synthetic GUS gene (PyGUS) with optimized codons for Porphyra yezoensis. Interestingly, the PtHSP70 promoter allowed the efficient expression of PyGUS in P. tenera and P. yezoensis, whereas the PyGAPDH promoter from P. yezoensis was not fully functional in P. tenera. The PtHSP70 promoter may have a more conserved regulatory mechanism than the PyGAPDH promoter between these species, suggesting that PtHSP70 could serve as a universal promoter for Porphyra species. We also established an efficient transient transformation system for P. tenera by evaluating transformation parameters including gold particle quantity, helium and vacuum pressure, developmental stages of leafy gametophytes, and target distance. Under optimal conditions of transient transformation, the frequency of GUS expression was determined by histochemical staining as 30-50 cells per bombardment. In addition, PyGUS expression was detected during the regeneration of monospores in P. tenera, indicating successful genetic transformation. Therefore, the new transient transformation system using the PtHSP70 promoter can be used for foreign gene expression in P. tenera, which may advance the development of P. tenera as a model organism. - 宇治利樹, 嵯峨直恆, 三上浩司 日本植物生理学会年会要旨集 52nd 206 2011年03月11日 [査読無し][通常論文]
- 橋潤, 宇治利樹, 嵯峨直恆, 三上浩司 日本植物生理学会年会要旨集 50th 372 2009年03月16日 [査読無し][通常論文]
- 宇治利樹, 三上浩司, 福田覚, 大場利治, 浅田起代蔵, 北出幸広, 遠藤博寿, 朴恩貞, 加藤郁之進, 嵯峨直恆 日本植物生理学会年会要旨集 49th 2008年
- 北出幸広, 中村理湖, 宇治利樹, 福田覚, 遠藤博寿, 嵯峨直恆 生化学 2007年
書籍等出版物
- Emergence of Ectocarpus as a model system to study the evolution of complex multicellularity in the brown algae. In: "Evolutionary transitions to multicellular life"Cock J.M, Godfroy O, Strittmatter M, Scornet D, Uji T, Farnham G, Peters A.F. Coelho S
Springer, pp 153-162, (2015) - Transient gene expression systems in Porphyra yezoensis: establishment, application and limitation.Porphyra yezoensis: Frontiers in Physiological and Molecular Biological Research.Mikami K, Uji T
New York: Nova Science Publishers, pp 93-117. (2012) - Transient transformation of red algal cells: Breakthrough toward genetic transformation of marine crop Porphyra species.Mikami K, Hirata R, Takahashi M, Uji T, Saga N
InTech-Open Access Publisher, pp 241-258. (2011)
所属学協会
Works（作品等）
共同研究・競争的資金等の研究課題
- エチレン前駆物質による紅藻スサビノリの有性生殖の制御機構に関する研究日本学術振興会：科学研究費助成事業 若手研究研究期間 : 2019年04月 -2022年03月代表者 : 宇治 利樹
- アミノ酸添加による有用海藻類の健全かつ効率的な種苗生産技術の開発栗林育英学術財団：令和2年度研究助成（個人研究の部）研究期間 : 2020年09月 -2021年09月
- 岩海苔の安定的な増養殖を目指した胞子形成および放出機構の解明ノーステック財団：若手研究人材・ネットワーク育成補助金研究期間 : 2020年09月 -2021年03月
- 北方系海藻の通年収穫を目的とした陸上栽培技術の開発ノーステック財団 令和元年度（2019年度）「研究開発助成事業」 スタートアップ研究補助金：研究期間 : 2019年08月 -2020年03月代表者 : 川越 力, 水田浩之, 宇治利樹, 木下康宣, 岸村栄毅, 熊谷祐也
- 日本学術振興会：科学研究費助成事業 若手研究(B)研究期間 : 2016年04月 -2019年03月代表者 : 宇治 利樹本研究では、海苔の原料であるスサビノリの有性生殖や酸化ストレス応答にエチレンではなく、その前駆物質である1-アミノシクロプロパン-1-カルボン酸(ACC)が関与していることを明らかにし、海藻において初めて、ACC依存的なシグナル伝達系の存在の可能性を示した。またその応答にはNADPHオキシダーゼ由来の活性酸素種（ROS）が関与していることも見出した。さらにACC応答性遺伝子として、小胞体輸送や細胞分裂、抗酸化に関連する遺伝子を同定し、これらの遺伝子が本種の有性生殖や抗酸化獲得に関与している可能性が明らかとなった。
- 「次世代型アカモク養殖を目指したビックデータ構築と種苗生産の確立COI拠点「食と健康の達人」拠点 」研究期間 : 2018年12月 -2019年03月代表者 : 宇治 利樹
- 海苔の有用品種作出に向けた紅藻スサビノリにおける高温応答機構の解明日本学術振興会：科学研究費助成事業 特別研究員奨励費研究期間 : 2015年04月 -2016年03月代表者 : 宇治 利樹本研究では当初、エチレン（ACC）と高温耐性の関連に焦点を当てて解析を行ったが、高温耐性以外の効果について以下のことが明らかとなった。スサビノリ由来のACC合成酵素遺伝子PyACSが銅およびH2O2ストレス条件下において発現が誘導されることから、エチレンがこれらのストレスに対して応答するために重要な機能をしている可能性が示唆された。そこで、ACC処理による銅ストレスやH2O2ストレス耐性の効果を調べたところ、未処理の藻体と比較して、ACC処理藻体では大量の果胞子様細胞を形成することにより、これらのストレスに適応することが明らかとなった。また長期間（２週間以上）のACC処理により、光合成色素タンパク質であるフィコエリスリン、フィコシアニンおよびクロロフィルの分解が促進されること、生殖細胞の形成が促進されることが明らかとなった。そのため、エチレンの応答機構を解明することにより、高温耐性等の環境ストレス応答機構に加えて、色落ちや有性成熟の機構の解明につながることが期待される。そしてこれらの知見は海苔の品種改良を進めていく上で重要な知見になることが予想される。
またエチレンのスサビノリに対する作用機序に関する知見を得るために、エチレンによって制御されている遺伝子群の網羅的な同定を試みた。次世代シーケンサー（イルミナ社Hiseq2000）を用いてRNA-seq解析を行った結果、ストレス耐性に関与する候補遺伝子として、カタラーゼ、分子シャペロン（DnaJ）およびプロテインキナーゼ遺伝子等の発現が、ACC処理藻体サンプルにおいて上昇することが明らかとなった。またエチレン応答性遺伝子として、RWP-RKドメインを持つ遺伝子を見出した。この遺伝子は他の生物において転写調節因子をコードすることから、スサビノリにおいても重要な機能を持つ可能性が考えられた。 - 褐藻シオミドロの性分化と成熟機構の解明上原記念生命科学財団：平成23年度海外留学助成ポストドクトラルフェローシップ研究期間 : 2011年 -2012年代表者 : 宇治 利樹
- 多細胞真核生物における世代交代の多様化を制御する分子機構の研究北海道大学クラーク記念財団：平成21年度博士後期課程在学生研究助成研究期間 : 2009年 -2010年代表者 : 宇治 利樹
- 雌雄異株アマノリ属を用いた海産紅藻類における性決定機構の解明秋山生命科学振興財団：2017年度研究助成奨励代表者 : 宇治 利樹